LOCUS       DAJTYM010000001        94894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTYM010000001 DAJTYM010000000
VERSION     DAJTYM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 94894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1066)
                     /gene="icd"
                     /locus_tag="OO575_000001"
     CDS             complement(<1..1066)
                     /gene="icd"
                     /locus_tag="OO575_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6663119.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGS"
     gene            1238..1891
                     /gene="rluE"
                     /locus_tag="OO575_000002"
     CDS             1238..1891
                     /gene="rluE"
                     /locus_tag="OO575_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6663120.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            1901..2362
                     /gene="nudJ"
                     /locus_tag="OO575_000003"
     CDS             1901..2362
                     /gene="nudJ"
                     /locus_tag="OO575_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6663121.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2416..3522
                     /gene="mnmA"
                     /locus_tag="OO575_000004"
     CDS             2416..3522
                     /gene="mnmA"
                     /locus_tag="OO575_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6663122.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3558..4199
                     /gene="hflD"
                     /locus_tag="OO575_000005"
     CDS             3558..4199
                     /gene="hflD"
                     /locus_tag="OO575_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6663123.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4203..5573
                     /gene="purB"
                     /locus_tag="OO575_000006"
     CDS             4203..5573
                     /gene="purB"
                     /locus_tag="OO575_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6663124.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5742..6413
                     /gene="phoP"
                     /locus_tag="OO575_000007"
     CDS             5742..6413
                     /gene="phoP"
                     /locus_tag="OO575_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6663125.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6413..7873
                     /gene="phoQ"
                     /locus_tag="OO575_000008"
     CDS             6413..7873
                     /gene="phoQ"
                     /locus_tag="OO575_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6663126.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8670..8951)
                     /locus_tag="OO575_000009"
     CDS             complement(8670..8951)
                     /locus_tag="OO575_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663127.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(8965..10626)
                     /locus_tag="OO575_000010"
     CDS             complement(8965..10626)
                     /locus_tag="OO575_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6663128.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVAREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10610..10936)
                     /locus_tag="OO575_000011"
     CDS             complement(10610..10936)
                     /locus_tag="OO575_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6663129.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(10992..11174)
                     /locus_tag="OO575_000012"
     CDS             complement(10992..11174)
                     /locus_tag="OO575_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663130.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11158..11598)
                     /locus_tag="OO575_000013"
     CDS             complement(11158..11598)
                     /locus_tag="OO575_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6663131.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11598..11894)
                     /locus_tag="OO575_000014"
     CDS             complement(11598..11894)
                     /locus_tag="OO575_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6663132.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(11891..12229)
                     /locus_tag="OO575_000015"
     CDS             complement(11891..12229)
                     /locus_tag="OO575_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6663133.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12226..13437)
                     /locus_tag="OO575_000016"
     CDS             complement(12226..13437)
                     /locus_tag="OO575_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6663134.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13439..14011)
                     /locus_tag="OO575_000017"
     CDS             complement(13439..14011)
                     /locus_tag="OO575_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6663135.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14051..15208)
                     /locus_tag="OO575_000018"
                     /pseudo
     CDS             complement(14051..15208)
                     /locus_tag="OO575_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15509..15733)
                     /locus_tag="OO575_000019"
     CDS             complement(15509..15733)
                     /locus_tag="OO575_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663136.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(15875..16036)
                     /locus_tag="OO575_000020"
     CDS             complement(15875..16036)
                     /locus_tag="OO575_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6663137.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16046..16351)
                     /locus_tag="OO575_000021"
     CDS             complement(16046..16351)
                     /locus_tag="OO575_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663138.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16348..16626)
                     /locus_tag="OO575_000022"
     CDS             complement(16348..16626)
                     /locus_tag="OO575_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663139.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16915..18663)
                     /locus_tag="OO575_000023"
     CDS             complement(16915..18663)
                     /locus_tag="OO575_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6663140.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18660..18959)
                     /locus_tag="OO575_000024"
     CDS             complement(18660..18959)
                     /locus_tag="OO575_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6663141.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18965..19198)
                     /locus_tag="OO575_000025"
     CDS             complement(18965..19198)
                     /locus_tag="OO575_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663142.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19200..19420)
                     /locus_tag="OO575_000026"
                     /pseudo
     CDS             complement(19200..19420)
                     /locus_tag="OO575_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19413..19634)
                     /locus_tag="OO575_000027"
     CDS             complement(19413..19634)
                     /locus_tag="OO575_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663143.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19624..19824)
                     /locus_tag="OO575_000028"
     CDS             complement(19624..19824)
                     /locus_tag="OO575_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6663144.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19954..20133
                     /locus_tag="OO575_000029"
     CDS             19954..20133
                     /locus_tag="OO575_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663145.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20258..20587
                     /locus_tag="OO575_000030"
     CDS             20258..20587
                     /locus_tag="OO575_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663146.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20637..20825)
                     /locus_tag="OO575_000031"
     CDS             complement(20637..20825)
                     /locus_tag="OO575_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6663147.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21200..22429)
                     /locus_tag="OO575_000032"
     CDS             complement(21200..22429)
                     /locus_tag="OO575_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6663148.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22678..23799
                     /gene="roxA"
                     /locus_tag="OO575_000033"
                     /gene_synonym="ycfD"
     CDS             22678..23799
                     /gene="roxA"
                     /locus_tag="OO575_000033"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6663149.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDMYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23848..25074)
                     /gene="pepT"
                     /locus_tag="OO575_000034"
     CDS             complement(23848..25074)
                     /gene="pepT"
                     /locus_tag="OO575_000034"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6663150.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25324..26460
                     /gene="potA"
                     /locus_tag="OO575_000035"
     CDS             25324..26460
                     /gene="potA"
                     /locus_tag="OO575_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6663151.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26444..27301
                     /gene="potB"
                     /locus_tag="OO575_000036"
     CDS             26444..27301
                     /gene="potB"
                     /locus_tag="OO575_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6663152.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27298..28092
                     /gene="potC"
                     /locus_tag="OO575_000037"
     CDS             27298..28092
                     /gene="potC"
                     /locus_tag="OO575_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6663153.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28089..29135
                     /gene="potD"
                     /locus_tag="OO575_000038"
     CDS             28089..29135
                     /gene="potD"
                     /locus_tag="OO575_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6663154.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29291..30112)
                     /gene="cobB"
                     /locus_tag="OO575_000039"
     CDS             complement(29291..30112)
                     /gene="cobB"
                     /locus_tag="OO575_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6663155.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30128..31039)
                     /gene="nagK"
                     /locus_tag="OO575_000040"
     CDS             complement(30128..31039)
                     /gene="nagK"
                     /locus_tag="OO575_000040"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6663156.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31068..32312)
                     /gene="lolE"
                     /locus_tag="OO575_000041"
     CDS             complement(31068..32312)
                     /gene="lolE"
                     /locus_tag="OO575_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6663157.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32312..33013)
                     /gene="lolD"
                     /locus_tag="OO575_000042"
     CDS             complement(32312..33013)
                     /gene="lolD"
                     /locus_tag="OO575_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6663158.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33006..34205)
                     /gene="lolC"
                     /locus_tag="OO575_000043"
     CDS             complement(33006..34205)
                     /gene="lolC"
                     /locus_tag="OO575_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6663159.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34467..35540
                     /locus_tag="OO575_000044"
     CDS             34467..35540
                     /locus_tag="OO575_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6663160.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35668..39114
                     /gene="mfd"
                     /locus_tag="OO575_000045"
     CDS             35668..39114
                     /gene="mfd"
                     /locus_tag="OO575_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6663161.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39257..40219
                     /gene="ldtC"
                     /locus_tag="OO575_000046"
     CDS             39257..40219
                     /gene="ldtC"
                     /locus_tag="OO575_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6663162.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40302..40514)
                     /locus_tag="OO575_000047"
     CDS             complement(40302..40514)
                     /locus_tag="OO575_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6663163.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40799..41431
                     /gene="comR"
                     /locus_tag="OO575_000048"
     CDS             40799..41431
                     /gene="comR"
                     /locus_tag="OO575_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6663164.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41493..42032)
                     /locus_tag="OO575_000049"
     CDS             complement(41493..42032)
                     /locus_tag="OO575_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6663165.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42259..43563)
                     /gene="ndh"
                     /locus_tag="OO575_000050"
     CDS             complement(42259..43563)
                     /gene="ndh"
                     /locus_tag="OO575_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6663166.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(43971..44513)
                     /locus_tag="OO575_000051"
     CDS             complement(43971..44513)
                     /locus_tag="OO575_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6663167.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44536..45561)
                     /gene="nagZ"
                     /locus_tag="OO575_000052"
     CDS             complement(44536..45561)
                     /gene="nagZ"
                     /locus_tag="OO575_000052"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6663168.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45572..46396)
                     /gene="thiK"
                     /locus_tag="OO575_000053"
     CDS             complement(45572..46396)
                     /gene="thiK"
                     /locus_tag="OO575_000053"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6663169.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46377..47018)
                     /gene="lpoB"
                     /locus_tag="OO575_000054"
     CDS             complement(46377..47018)
                     /gene="lpoB"
                     /locus_tag="OO575_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6663170.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47033..47410)
                     /locus_tag="OO575_000055"
     CDS             complement(47033..47410)
                     /locus_tag="OO575_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6663171.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47413..47772)
                     /gene="hinT"
                     /locus_tag="OO575_000056"
     CDS             complement(47413..47772)
                     /gene="hinT"
                     /locus_tag="OO575_000056"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6663172.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48105..50290
                     /gene="fhuE"
                     /locus_tag="OO575_000057"
                     /pseudo
     CDS             48105..50290
                     /gene="fhuE"
                     /locus_tag="OO575_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50350..51783)
                     /gene="ptsG"
                     /locus_tag="OO575_000058"
     CDS             complement(50350..51783)
                     /gene="ptsG"
                     /locus_tag="OO575_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6663173.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52078..52875)
                     /locus_tag="OO575_000059"
     CDS             complement(52078..52875)
                     /locus_tag="OO575_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6663174.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52886..53890)
                     /gene="holB"
                     /locus_tag="OO575_000060"
     CDS             complement(52886..53890)
                     /gene="holB"
                     /locus_tag="OO575_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6663175.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53887..54528)
                     /locus_tag="OO575_000061"
     CDS             complement(53887..54528)
                     /locus_tag="OO575_000061"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6663176.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54518..55540)
                     /gene="yceG"
                     /locus_tag="OO575_000062"
     CDS             complement(54518..55540)
                     /gene="yceG"
                     /locus_tag="OO575_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6663177.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55543..56352)
                     /gene="pabC"
                     /locus_tag="OO575_000063"
     CDS             complement(55543..56352)
                     /gene="pabC"
                     /locus_tag="OO575_000063"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6663178.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56472..57713)
                     /gene="fabF"
                     /locus_tag="OO575_000064"
     CDS             complement(56472..57713)
                     /gene="fabF"
                     /locus_tag="OO575_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6663179.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57800..58036)
                     /gene="acpP"
                     /locus_tag="OO575_000065"
     CDS             complement(57800..58036)
                     /gene="acpP"
                     /locus_tag="OO575_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6663180.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58247..58981)
                     /gene="fabG"
                     /locus_tag="OO575_000066"
     CDS             complement(58247..58981)
                     /gene="fabG"
                     /locus_tag="OO575_000066"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6663181.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(58994..59923)
                     /gene="fabD"
                     /locus_tag="OO575_000067"
     CDS             complement(58994..59923)
                     /gene="fabD"
                     /locus_tag="OO575_000067"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6663182.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(59939..60892)
                     /gene="fabH"
                     /locus_tag="OO575_000068"
     CDS             complement(59939..60892)
                     /gene="fabH"
                     /locus_tag="OO575_000068"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6663183.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(60960..62030)
                     /gene="plsX"
                     /locus_tag="OO575_000069"
     CDS             complement(60960..62030)
                     /gene="plsX"
                     /locus_tag="OO575_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6663184.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62111..62284)
                     /gene="rpmF"
                     /locus_tag="OO575_000070"
     CDS             complement(62111..62284)
                     /gene="rpmF"
                     /locus_tag="OO575_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6663185.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62336..62857)
                     /gene="yceD"
                     /locus_tag="OO575_000071"
     CDS             complement(62336..62857)
                     /gene="yceD"
                     /locus_tag="OO575_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6663186.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63056..63640
                     /gene="yceF"
                     /locus_tag="OO575_000072"
     CDS             63056..63640
                     /gene="yceF"
                     /locus_tag="OO575_000072"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6663187.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63752..64711)
                     /gene="rluC"
                     /locus_tag="OO575_000073"
     CDS             complement(63752..64711)
                     /gene="rluC"
                     /locus_tag="OO575_000073"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6663188.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65284..68475
                     /gene="rne"
                     /locus_tag="OO575_000074"
     CDS             65284..68475
                     /gene="rne"
                     /locus_tag="OO575_000074"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6663189.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68672..69614)
                     /gene="flgL"
                     /locus_tag="OO575_000075"
                     /pseudo
     CDS             complement(68672..69614)
                     /gene="flgL"
                     /locus_tag="OO575_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69626..71268)
                     /gene="flgK"
                     /locus_tag="OO575_000076"
                     /pseudo
     CDS             complement(69626..71268)
                     /gene="flgK"
                     /locus_tag="OO575_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71334..72275)
                     /gene="flgJ"
                     /locus_tag="OO575_000077"
     CDS             complement(71334..72275)
                     /gene="flgJ"
                     /locus_tag="OO575_000077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6663190.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72275..73372)
                     /gene="flgI"
                     /locus_tag="OO575_000078"
     CDS             complement(72275..73372)
                     /gene="flgI"
                     /locus_tag="OO575_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6663191.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73384..74082)
                     /gene="flgH"
                     /locus_tag="OO575_000079"
     CDS             complement(73384..74082)
                     /gene="flgH"
                     /locus_tag="OO575_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6663192.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74135..74917)
                     /gene="flgG"
                     /locus_tag="OO575_000080"
     CDS             complement(74135..74917)
                     /gene="flgG"
                     /locus_tag="OO575_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6663193.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75055..75806)
                     /gene="flgF"
                     /locus_tag="OO575_000081"
                     /pseudo
     CDS             complement(75055..75806)
                     /gene="flgF"
                     /locus_tag="OO575_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(75826..76965)
                     /locus_tag="OO575_000082"
     CDS             complement(75826..76965)
                     /locus_tag="OO575_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6663194.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(76990..77685)
                     /gene="flgD"
                     /locus_tag="OO575_000083"
     CDS             complement(76990..77685)
                     /gene="flgD"
                     /locus_tag="OO575_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6663195.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77697..78100)
                     /gene="flgC"
                     /locus_tag="OO575_000084"
                     /pseudo
     CDS             complement(77697..78100)
                     /gene="flgC"
                     /locus_tag="OO575_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78104..78520)
                     /gene="flgB"
                     /locus_tag="OO575_000085"
     CDS             complement(78104..78520)
                     /gene="flgB"
                     /locus_tag="OO575_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6663196.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78675..79334
                     /gene="flgA"
                     /locus_tag="OO575_000086"
     CDS             78675..79334
                     /gene="flgA"
                     /locus_tag="OO575_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6663197.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79410..79703
                     /gene="flgM"
                     /locus_tag="OO575_000087"
     CDS             79410..79703
                     /gene="flgM"
                     /locus_tag="OO575_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6663198.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79708..80124
                     /gene="flgN"
                     /locus_tag="OO575_000088"
     CDS             79708..80124
                     /gene="flgN"
                     /locus_tag="OO575_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6663199.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80164..81699)
                     /gene="murJ"
                     /locus_tag="OO575_000089"
     CDS             complement(80164..81699)
                     /gene="murJ"
                     /locus_tag="OO575_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6663200.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(81899..82732)
                     /locus_tag="OO575_000090"
     CDS             complement(81899..82732)
                     /locus_tag="OO575_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6663201.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(82734..83381)
                     /locus_tag="OO575_000091"
     CDS             complement(82734..83381)
                     /locus_tag="OO575_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6663202.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83392..83976)
                     /gene="rimJ"
                     /locus_tag="OO575_000092"
     CDS             complement(83392..83976)
                     /gene="rimJ"
                     /locus_tag="OO575_000092"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6663203.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84212..85420
                     /gene="mdtH"
                     /locus_tag="OO575_000093"
     CDS             84212..85420
                     /gene="mdtH"
                     /locus_tag="OO575_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6663204.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85484..86131
                     /gene="grxB"
                     /locus_tag="OO575_000094"
     CDS             85484..86131
                     /gene="grxB"
                     /locus_tag="OO575_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6663205.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86265..86825
                     /locus_tag="OO575_000095"
     CDS             86265..86825
                     /locus_tag="OO575_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6663206.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            86931..87977
                     /gene="pyrC"
                     /locus_tag="OO575_000096"
     CDS             86931..87977
                     /gene="pyrC"
                     /locus_tag="OO575_000096"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6663207.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88051..88296
                     /gene="dinI"
                     /locus_tag="OO575_000097"
     CDS             88051..88296
                     /gene="dinI"
                     /locus_tag="OO575_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6663208.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88586..88840
                     /gene="bssS"
                     /locus_tag="OO575_000098"
     CDS             88586..88840
                     /gene="bssS"
                     /locus_tag="OO575_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6663209.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            88955..90073
                     /gene="solA"
                     /locus_tag="OO575_000099"
     CDS             88955..90073
                     /gene="solA"
                     /locus_tag="OO575_000099"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6663210.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90121..90234
                     /locus_tag="OO575_000100"
     CDS             90121..90234
                     /locus_tag="OO575_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6663211.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90495..91061
                     /locus_tag="OO575_000101"
     CDS             90495..91061
                     /locus_tag="OO575_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6663212.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91065..91640
                     /locus_tag="OO575_000102"
     CDS             91065..91640
                     /locus_tag="OO575_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6663213.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91682..92734)
                     /locus_tag="OO575_000103"
     CDS             complement(91682..92734)
                     /locus_tag="OO575_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6663214.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            92959..93879
                     /gene="lpxL"
                     /locus_tag="OO575_000104"
     CDS             92959..93879
                     /gene="lpxL"
                     /locus_tag="OO575_000104"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6663215.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94051..94248
                     /locus_tag="OO575_000105"
     CDS             94051..94248
                     /locus_tag="OO575_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6663216.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94272..>94894
                     /locus_tag="OO575_000106"
     CDS             94272..>94894
                     /locus_tag="OO575_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6663217.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGL"
BASE COUNT        22804 a        25172 c        23435 g        23483 t
ORIGIN      
        1 tagagccagg gtttactttg tcctgaccgg cgtatttcgg cgcagtaccg tgggtggctt
       61 caaacagggc gcattcgtca ccgatgtttg ctccaggggc gataccgata ccgccaacct
      121 gcgctgccag agcgtcagaa atgtagtcac cgttcaggtt catacaggcg ataacatcat
      181 attcagccgg acgcagcagg atctgttgca ggaatgcatc agcaatcacg tctttaatga
      241 cgatctcttt gccggtattc gggtttttaa ctttcagcca cgggccgccg tcgatcagtt
      301 caccgccaaa ttcttcacgc gccagctggt agccccagtc tttaaacgcg ccttcggtga
      361 acttcatgat gttgcctttg tgcaccaggg tcacagagtc acgatcgtta gcaattgcgt
      421 attcgatcgc tgcacgaacc agacgtttgg tgccttcttc agaacacggc ttaataccga
      481 taccgcaatg ttccgggaag cgaattttct tcacgcccat ctcttcacgc aggaatttaa
      541 tcactttctc ggcgtcggca gagtcagctt tccattcgat acccgcataa atgtcttccg
      601 agttttcacg gaagataacc atatcggtca gttcagggtg tttaaccggg cttggagtgc
      661 cctgatagta acgtaccgga cgcaggcaga tgtagagatc cagttcctga cgcagggcaa
      721 cgttgagaga acgaataccg ccaccaaccg gagtggtcag cgggccttta atggcaacgc
      781 gatattcacg aatcagatca agggtttcag caggcagcca gacatcctga ccataaacct
      841 gtgtggattt ttcaccggtg taaatttcca tccaggagat tttacgctcg cctttatagg
      901 ctttctcgac tgcagcgtcg accactttca gcatggctgg ggttacatct acaccgattc
      961 catcaccttc aatgtaaggg ataatcggat tttcaggaac gttgagtttg ccgttttgca
     1021 gggtgatctt cttgccttgt gccggaacaa ctactttact ttccattcac ctctccttcg
     1081 agcgctactg gtttgctcgt ctgccaccac gttgcatatg cgtttgcgtc ctgcgatacg
     1141 gatgctttag agcaattttt tgttaatgat ttgtaattga cttgtcaata ctaccctatt
     1201 gtttgccgtc atgaaagatg cgcgttatta ggctataatg cggcaattca taatctctga
     1261 aaataccatg caaaaaactt cttttagaaa tcaccaggtt aagcgattca gctcgcaacg
     1321 ttctaccagg cgtaaacatg aaaaccagcc cacgcgtgtg atcctcttca ataaacccta
     1381 cgatgttctt ccgcagttca ccgatgaaac cggacgcaaa acattaaaag aattcatccc
     1441 ggttcagggc gtttatgcag caggtcgcct tgaccgcgat agcgaagggt tgctggtgct
     1501 gaccaataac ggcgcgttgc aggcgcgttt aacccagccg ggtaaacgca ccggaaaaat
     1561 ctattatgtg caggtggaag gtattcccac acaagacgca cttgaagcct tgcgcaatgg
     1621 cgtaacctta aatgatggcc ctaccctgcc cgcaggcgcg gagctggttg acgaacccgc
     1681 gtggttatgg ccgcggaatc caccaattcg tgaacgcaaa agtattccca ccagctggct
     1741 gaagatcacc ttatatgaag gacgtaatcg ccaggtgcgc cgcatgaccg cccatgttgg
     1801 tttccccacg ctgcgactga ttcgctatgc gatgggagat tactctttgg ataatcttgc
     1861 caatggcgaa tggcgagaag tgacagatta aggatgcaaa atgtttaaac cgcacgttac
     1921 cgttgcttgc gtggtgcacg cagaaggcaa atttttagtc gttgaagaga cgattaatgg
     1981 taaagcgtta tggaaccaac ctgccgggca tctggaagcc gatgaaacct tagtggaagc
     2041 cgccgcccgt gaactgtggg aagaaaccgg catcagcgcg cagccgcaac actttattcg
     2101 tatgcatcag tggattgcgc cagataaaac gccgtttttg cgtttcctct ttgccattga
     2161 gcttgagcaa atatgcccga cccagcctca tgacagcgat atcgactgct gccgttgggt
     2221 cagcgccgaa gaaattttac aggcgtcaaa tcttcgttcg ccgctggtgg cggaaagtat
     2281 tcgttgttat caaagcgggc aacgttatcc gctggagatg attggcgatt ttaactggcc
     2341 ttttacaaag ggtgtcatct aaaaggctgc gtgatagaat acgccgcctt gaagttcaat
     2401 gtcgtgagtg atccaatgtc tgaaaccgca aaaaaagtaa tcgtcggcat gtccggcggt
     2461 gtcgattcct ccgtttctgc ctggctgttg caacaacagg gatatcaggt cgaaggcctg
     2521 tttatgaaga actgggaaga agacgacggt gaggaatatt gcacagcagc agcggatctg
     2581 gctgatgccc aggcggtctg cgacaagctc ggcattgaac tgcacaccgt taactttgct
     2641 gccgagtact gggacaacgt cttcgaactg ttccttgccg aatataaagc cggtcgcacg
     2701 ccgaatccgg atattctgtg caacaaggag atcaaattta aagccttcct cgaatttgcc
     2761 gccgaagatt taggtgccga ttatatcgct accggtcatt acgtacgtcg ggccgatgtc
     2821 gatggcaaga gccgcctgct gcgtggtctg gacagcaata aagaccagag ctacttcctt
     2881 tatacgctca gccatgagca gattgcgcaa agcctgttcc cggtaggcga actggaaaaa
     2941 ccgcaggtgc gtaagattgc tgaagatctt ggtctggtca ccgcgaagaa aaaagactct
     3001 accggcattt gcttcattgg cgaacgtaaa ttccgcgagt tcctgggccg ttatctcccg
     3061 gcgcaaccgg gcaaaatcat taccgtcgat ggcgatgaaa ttggtgagca ccaggggctg
     3121 atgtatcaca ctctcggtca gcgtaaaggt ctgggtatcg gtggcaccaa agaaggtacc
     3181 gaagaaccgt ggtatgtggt ggacaaagac gtcgaaaaca acattctggt tgtcgctcag
     3241 ggccatgaac acccgcggct gatgtctgtc gggttgattg cccagcagct gcactgggtc
     3301 gatcgcgaac cattcaccgg cactatgcgt tgcacggtaa aaacccgcta tcgccagacc
     3361 gacattccgt gcaccgtcaa ggcgctggac gacgatcgca ttaaagtgat tttcgatgag
     3421 ccggttgccg ccgtgacgcc gggccagtct gccgtcttct ataacggtga agtgtgcctc
     3481 ggtggcggta ttattgagca gcgtctgccg ctgccggtct gattattatc tttacttaaa
     3541 acaaggaagc agtgaacgtg gcaaagaatt actatgacat caccctcgcc ctggccggta
     3601 tttgtcagtc ggcacgcctg gtgcaacaac tcgctcacca ggggcattgt gatgccgatg
     3661 cgctacacgt ctcactcaac agtattattg atatgaaccc cagctcgacg ctggcggttt
     3721 ttggcggtag cgaagccaac ctgcgcgtcg ggctggaaac cctgctcggc gtgctcaatg
     3781 ccagcagttg ccagggctta aacgccgaat taacccgcta cacactcagc ttgatggtgc
     3841 ttgagcgcaa actctcctca gcgaaaggcg cgctcgacac tctgggcaac cggatcaacg
     3901 gcctgcaacg ccagctcgaa cacttcgatt tacagtccga aacgctgatg agcgcgatgg
     3961 ctgctatcta tgttgatgtg atcagcccgc ttggcccgcg cattcaggtc accggttccc
     4021 ctgctgtact gcaaagccca caagtgcagg cgaaagttcg cgcaaccctg ctggcaggca
     4081 ttcgcgccgc cgtgctctgg caccaggtcg gcggcggacg tctacaactg atgttttctc
     4141 gtaatcgcct gaccactcag gcaaaacaaa ttcttgctca tttaaccccg gagttgtgat
     4201 ctatggaatt atcctcactg accgccgttt cccctgtcga tggacgctac ggcgataaag
     4261 tcagcgcgct gcgcgggatt ttcagcgaat atggtttgct gaaattccgt gtacaagttg
     4321 aagtacgttg gctgcaaaaa ctggccgcgc acgcagcgat caaggaagtt cctgcttttg
     4381 ctgccgacgc aatcggttac cttgatgcaa ttgtcgccaa tttcagcgaa gaagatgccg
     4441 cacgcatcaa aaccatcgag cgtaccacta accacgacgt taaagcggtt gagtatttcc
     4501 tgaaagaaaa agtggcggag atcccggaac tgcacgcggt ttctgaattc atccactttg
     4561 cctgtacttc ggaagatatc aataacctct cccacgcatt aatgctgaaa accgcgcgtg
     4621 atgaagtgat cctgccgtac tggcgtcaac tgattgatgg cattaaagat ctcgccgttc
     4681 agtaccgcga tatcccgctg ctgtctcgta cccacggtca gccagccacg ccgtcaacca
     4741 tcggtaaaga gatggcaaac gtcgcctacc gtatggagcg ccagcaccgc cagcttaacc
     4801 aggtggagat cctcggcaaa atcaacggcg cggtcggtaa ctataacgcc cacatcgccg
     4861 cttacccgga agttgactgg catcagttca gcgaagagtt cgtcacctcg ctgggtattc
     4921 aatggaaccc gtacactacc cagattgaac cgcacgacta cattgccgaa ctgtttgatt
     4981 gcgttgcgcg cttcaacacc attctgatcg actttgaccg tgacgtctgg ggttatatcg
     5041 cccttaacca cttcaaacag aaaaccattg ctggtgagat tggttcttcc accatgccgc
     5101 ataaagttaa cccgatcgac ttcgaaaact ccgaagggaa tctgggcctt tccaacgcgg
     5161 tattgcagca tctggcaagc aaactgccgg tttcccgctg gcagcgtgac ctgaccgact
     5221 ccaccgtgct gcgtaacctc ggcgtgggta tcggttatgc gctgattgcg tatcaatcca
     5281 ccctgaaagg cgtgagcaaa ctggaagtga accgtgacca tctgctggat gagctggatc
     5341 acaactggga agtgctggca gaaccaatcc agacagttat gcgtcgctat ggcatcgaaa
     5401 aaccgtacga gaagctgaaa gagcttactc gcggtaagcg cgttgacgcc gaaggcatga
     5461 agcagtttat cgatggtctg gcgttgccag aagaagagaa agcccgcctg aaagcgatga
     5521 cgccggctaa ctatattggt cgagctatca cgatggttga tgagctgaaa taagcctcaa
     5581 atcagtgccg gatggcgatg ctgtccggcc tgcttattaa gattatccac tttttctttt
     5641 ttcactttac ctcccctccc cgctggttta tttaatgttt acccccataa acacataatc
     5701 gcgttacact attttaataa ttaagacagg gagaaataaa aatgcgcgta ctggttgttg
     5761 aagacaatgc gttgttacgt caccacctta aagttcagat tcaggatgct ggtcatcagg
     5821 tcgatgacgc agaagatgcc aaagaagccg attattatct caatgaacat ttaccggata
     5881 ttgcgattgt cgatctcgga ttgcctgacg aggacggtct gtcactgatt cgccgctggc
     5941 gtagcaacga tgtttcactg ccgattctgg tattaaccgc ccgtgaaagc tggcaggaca
     6001 aagtcgaagt attaagtgcc ggtgctgatg attacgtgac taaaccgttt catattgaag
     6061 aggtgatggc gcgaatgcag gcattaatgc ggcgtaatag cggtctggct tcacaggtca
     6121 tttcgctccc cccgtttcag gttgatctct ctcgccgtga attatctatt aatgacgaag
     6181 tgatcaaact gaccgcgttc gaatacacca ttatggaaac gttgatacgc aataatggca
     6241 aagtggtcag caaagattcg ttaatgctac aactctatcc ggatgcggag ctgcgggaaa
     6301 gccataccat tgatgtactg atgggacgtc tgcgcaaaaa aattcaggca caatatccgc
     6361 aagaagtcat taccaccgtt cgcggccagg gctacctgtt cgaattgcgc tgatgaaaaa
     6421 attactgcgt ctttttttcc cgctctcgct gcgggtacgt tttctgttgg caacggcagc
     6481 ggtagtactg gtgctttcgc ttgcctacgg aatggtcgcg ctgatcggtt atagcgtcag
     6541 tttcgataaa actacgtttc ggctgttacg tggcgagagc aatctgttct atacccttgc
     6601 gcagtgggaa aacaataagt tgcatgtcga gttacccgaa aatatcgaca agcaaagccc
     6661 caccatgacg ctaatttatg atgagaacgg gcagctttta tgggcgcaac gtgacgtgcc
     6721 ctggctgatg aaaatgatcc agcctgactg gctgaaatcg aatggttttc atgaaattga
     6781 agcggatgtt aacgatacca gcctcttgct gagtggagat cattcgatac agcaacagtt
     6841 gcaggaagtg cgggaagatg atgacgacgc ggagatgacc cactcggtgg cggtaaacgt
     6901 ctacccggca acatcgcgga tgccaaaatt aaccattgtg gtggtggata ccattccggt
     6961 ggagctaaaa agttcctata tggtctggag ctggtttatc tatgtgctct cagccaatct
     7021 gctgttagtg atcccgctgc tgtgggtcgc cgcctggtgg agtttacgcc ccatcgaagc
     7081 cctggcaaaa gaagtccgcg aactggaaga acataaccgc gaattgctca atccagccac
     7141 aacgcgagaa ctgaccagtc tggtacgaaa cctgaaccga ttgttaaaaa gtgaacgcga
     7201 acgttacgac aaataccgta cgacgctcac cgacctgacc catagtctga aaacgccact
     7261 ggcggtgctg caaagtacgc tgcgttctct gcgtagtgaa aagatgagcg tcagtgatgc
     7321 tgagccggta atgctggagc aaatcagccg catttcacag caaattggct actacctgca
     7381 tcgtgccagt atgcgcggcg ggacattgct cagccgcgag ctgcatccgg tcgccccact
     7441 gctggacaat ctcacctcag cgctgaacaa agtgtatcaa cgcaaagggg tcaatatctc
     7501 tctcgatatt tcgccagaga tcagctttgt cggtgagcag aacgattttg tcgaggtgat
     7561 gggcaacgtg ctggataatg cctgtaaata ttgcctcgag tttgtcgaaa tttctgcaag
     7621 gcaaaccgac gagcatctct atattgtggt cgaggatgat ggccccggta ttccattaag
     7681 caagcgagag gtcattttcg accgtggtca acgggttgat actttacgcc ctgggcaagg
     7741 tgtagggctg gcggtagccc gcgaaatcac cgagcaatat gagggtaaaa tcgtcgccgg
     7801 agagagcatg ctgggcggtg cgcggatgga ggtgattttt ggtcgccagc attctgcgcc
     7861 gaaagatgaa taaatatgtc cttttaccac tacatcaagt cattgattcg atcaaacaat
     7921 tttcattgaa aaagtgccaa aaatcgcaaa ttgactacac cattaactac accgttcggt
     7981 gcactgtatg aaacaacgtg gaacaaatag acacaagaaa tatacaggcg ggtaatcttt
     8041 ccagggggaa gcgcaccaat tcatgagggg cgttaatgtc gatatgggga tccccataac
     8101 ggggctaccg gatttttttt ccggttaacc tttaatcagg ctggtgggtt ttacataccc
     8161 ttgatcaccg taatgatgat ccagatgtgc accttttcca ccaatggcgg aaaagctcca
     8221 accgtaacga tgatcgctat ggttgcctcg atggtctgcg tggtcattat gaccgttcag
     8281 ccagtgacct taatcagaat ttttcgctaa aaaatgacgt tggccacgtc atccggaaag
     8341 cacaaaatca cgtcgatttt taatgaacgg ttaaaccgtc tttttgtcac ggtgtgtcac
     8401 tggttgtcac tctccgtcac gattgaggtg gtggtcattc tgcccgaccg cgcatctttg
     8461 cgctatcggg aatatcaaac agttacctcg ccgacttcct caaattgagg tttccgaaaa
     8521 tatcagagcg ggttagcgat taagctgacg agtaattaaa ctgcgaaaat ttcgtagttt
     8581 ctgaatatat tccggcctgc tggttaacag tcctcagatg tgagggatgt agccagcgca
     8641 attttgcgct ttgctttaat ctcagtgagt taccgccgta accgctccgg cttcttccag
     8701 tggtacgtga ttttctcctt ctcccgatac agttcaacgc ggcgattgta ggccagcatt
     8761 tccagaacgc ggatccgtat gtcgcgcata tccgcatcat taagctggat accatcacgg
     8821 cgcatcacct cagcaacaac acgcgcataa ttttcggctg tcacgctgtc cggctgcgtg
     8881 gtctgttcgt cagcctgctg gctgattcca gagacgcggc ggattaatcg cagtatttcg
     8941 gcttctgtca tgctgctgac ctcattacac cccgctaaat tcttccagtt tctggcggtg
     9001 gctgtcgctt atatcaaaag caaaatcctc atgctctgcc tggaatgtac caaacgccat
     9061 cagcgccgct acgctcgggt ctatcttgtt cggtgatttt ttcttgttcg gcttgatatt
     9121 ggcgttcgcg tcactctgca tcacgacgtt actcatcgac caggacaaca ccggatcgcc
     9181 acgatgcaca atcaccctgc ggttaacaaa aacttcgaac gatttcgccg ccggactgaa
     9241 tctgaggtag gtttgcggga acggctccac ctcaaaacca gccccctgta attgcgtcct
     9301 gagatgcgtg gcgttccacg tatcaaagcc caccagcctg atattaaatt tctccgcatc
     9361 ctgcatgatg tcatctctga tccggtcgta atcaatgcag tcacctggcg ttgtgcgtat
     9421 ccagcccgct ttagcccact ggcgatagac agcgcggttt ttattggcgg ggttctgtag
     9481 ctggaactcc ggcagataat gacgggaaat cagcataatg gttttaccga ccggaaaggc
     9541 atagcatacg ctggaaatat cgctggttga tgataagtcc agccccgcgt aacactcctg
     9601 accgtgtaaa tcttcctccg tgaacgttcc ggcacactca gcccatgcac cattacccat
     9661 ccacggggta gtcccctgac accagatatt aaatcgcttt gtcatcatct ccacccactg
     9721 cgacggaata ccccgcgctt tctggatggt tgaggccagt ttttcacgag ccacggaaac
     9781 atcaatatta gggttcgcct ttatccacat cgccggatca tcaacctcgt tttcgtcgtc
     9841 cagttcgtaa atcagtacaa aaattgaatc gttaacctct tcgccgtcca ggatctggca
     9901 gcaataatca tagtgctgtt tacaggctga aacgacgtta ctgcccgatg tggtaatggc
     9961 aaataacagc ccttcgggac gtgcgcccat ccccagctca agcgcggaat aaacgccgtt
    10021 atcggggtgt aggtggtatt cgtccacgat ggaaagactg gggtttgtcc cctcgatggt
    10081 tgccgctttt gctgccagcg gctttaacag gctgttgctt ttcgggtgta tcaccttatg
    10141 cgcctgaata ttcacccgcc tgcgtaacgg tcgggataaa aggcacatct gacgcgcatt
    10201 atcaaacacg atccgcgcct ggtcacggct caccgctgcg gtgtagatat cctgctgacc
    10261 gttctccata atcagaaacc agttagccag aatcgcggcg gtcgtggatt tcgcattttt
    10321 gcgcggcact tcgataaagg cgctcgtgta tttgcgccgt ccggtggcct taaccttaaa
    10381 gccgaggatg cacgcaaagg cgaactgctg ccacggctcc agctcaatgg gtctgccacg
    10441 catcggtcct tttacgtgcg ggcacaccct ggaaaaggca ataaaccgct ccacaacctc
    10501 acgatcgaac gtgtaaaggg ggcttttaag gtccgaaaag taccgtttaa cagcctgttt
    10561 taaccgttta caggccggaa ttttgcccgt ttttacgtct tctgcgtact tattccaggc
    10621 ggtcaagctc gtcctcttct tctgtttccg gtggattttt acggcggctt atcgggtcaa
    10681 aaccgagcaa ggaggccatt tttatcatca ctctttcagc gtcggatttt gcgcttaatg
    10741 cggggtttct gctctcgccg ccctgactgt taacaatgct gaacccgcgc gccgcaaggt
    10801 ctgcaacggc tttccggtaa atggagtagt tgacgcaata cagttccaga ttgctccagt
    10861 cggcgggggt caggtctccc cgttccgcaa gctgccgcga tttttcccgc cactgcttca
    10921 cggcgatatc atccaggtag gcggggtcgg gtttgtcctg aaaacgcccc ccaccccgtc
    10981 atacagcctc atcagcgatt gcggaaacat tccataacct cgcggtcacg gtcggttaat
    11041 cgcttcgctg tgatgcgttc tgcgcgtcct gacgctttat ctttatgccc tgtttcctgt
    11101 gtcttccatg cgtcacgctg ccttataagt ccacggataa ggcggttttg ttcccgctca
    11161 ttcatcagcg tcatacatcc agttattgcg gttagcagcc cgttcttcct cttcacgaaa
    11221 tccacctgcg gcacgcttgc tttttgttgc cggatcaagc catttcgttt tctggttatg
    11281 acacgcctgg cacaatggtt gatggttcca ttcgggccag aagagaacat catcaccgcc
    11341 attaatcggg ataatgtgat ccaccaccac ggcgggcgta tatatcccct tctcaaggca
    11401 tcgcacgcat aacgggtttt tactcagata catggcgcgg tatttgtccc actgtctgga
    11461 atatccacgc gcgcggcggt gtcctcgtct ggcatcctct gcacgccatg ccgcccgcct
    11521 gtgctcctca cacttgccgg atttaacgcg cctgttacag cctggttctg tgcatcgtct
    11581 taatggttgc cacggcatca gtaccccccc acatcacggt aagccgtcca gagtgcgcct
    11641 atcgtcatgg gtacgcgtgt ttttgcgtta tccgcgacaa tctggcgatt ttcatacagg
    11701 tgagcgataa acatcatgca gccaatcttt atggctggcg tgaacgccag cccgtcatca
    11761 aagcgcctgc ctatgtgtat ctggcacgcc tcaagcgatg cggcaatgta tccgctgatt
    11821 aactggtctt catcgtcgcc atcgatgcgg caatggagtt tcacttcttc cagggtgata
    11881 agttcttctg tcatttttcc gcgccctcac gacaaagaat ttcaaggcgt gtcctggcgg
    11941 catccggcag cggctgcccg atgatattca gtacacgccc cgccagcggc cccgtactga
    12001 cctttatccg gctggtggcg ttgatgtcct tccggtaccg tatccagatc cttacggtac
    12061 caatcgccag ctctgcgccc gatgaaatgg cctccttgct gctgaccatg ttcacgcttg
    12121 cccagagtgt gtgaccgtcc tcccacgttt cgagtatttc gccggttata tccctggtct
    12181 gtttcagggt ctgaatcgtt atcctgtcac gcagtcgccc tatgttcatt ccggtttttc
    12241 tccctcgctg atttttactt cctgtttcca tgcctggctg aactcatcac caccatcacg
    12301 cggtgacagc ccttcacgtt cgcgggcttc gttcggacac ataacgtcgg attttatgcc
    12361 gcgctcatag gtggcaaagc gttcgccagg tgtggcccgt aacaggtccg cgctgtcaaa
    12421 ctccacctga taccggatac caggtacagg cgaggccacc agcaaggcgt tttttatctg
    12481 ctgctcaaag ttcgccagcc acgggcgcat cgtcatggtg agaaatgcgc ggcttgcctc
    12541 gctgaagttg ctgtaggtgc tgttgctgta ttcctgcaaa aaaatcggcg agacgttgaa
    12601 cattcgggcg atgtcttcaa tggtgaagcg acgggaggcc agccattcgg catcctgatt
    12661 actcatgccc agctgctgat agctcatccc cccttcaagg atgggcgttt ttccggcgtt
    12721 tctggcccct ttgtagcgtt ccagtgcggc taatgcctgt ttgcccttca cgccgtccag
    12781 ccattcgcct gacgtgataa cccctgccgc catcatgcca tcacgcatca cgctcgcgcc
    12841 gtggcgttgt tgggccagcc caagccccag cgattcgcgg cagatggtta caggtgagcg
    12901 ccccataaaa ccgtcatccg tggagtaacg aaggtggaga acctcccacg gtaaatagtt
    12961 gcggatgttt ccggtgtagg cgtcagtgat gcagtagcgc cagttatgat cacctgtctg
    13021 ctccacgttc accgactgcg gcgggtaagg atgtaaagcc gccggaaaac catcacgccc
    13081 ccactgaata accgcataag cattaccact taacaggcag tagcggatca tcattcgctt
    13141 aaactggtag ggtgtctgcc acgcgttcgg gcgctcgttg aggatgtgat cgaccggatg
    13201 agaatcaagc cattcgcggg cctccttccc cttctcattg cgtaccaggt acaggtaaca
    13261 cggcatggta gccaccgcgt cagagatgac cgtgacggcg ttcatgacgg cggggagtga
    13321 ttccgctgtc ccagatgata cgtactcacc cgcccccgtg ttcgatgtgc cggccagcgc
    13381 cataaattca tcaagcgtca tgctgcgctg ctcttttttt cgtctgaaag gccacatatc
    13441 acacccccgc taaatccgcc caccagcggc gattatccgc acgcggcatt ttttcggggt
    13501 gctgctcata cagggaacgg cgggccagct ccacgccgga atcggggtaa gccggtaccg
    13561 atgtaacggt aatttcgtac agttccgcca ccagcacggt gcgcacgcat ggatctgttg
    13621 tggtatccca tacatcctta cgggagcgaa agccaaagct catccccgag atgtccccac
    13681 gcttaaccag ttcgataacg tcgcgccctg tactggtatc cggtggggtc agttcaaagc
    13741 gtaaccctgt ttcgtcctct tccagtttca gcgtgccgga acgggtgcgc cccagtaaca
    13801 tgctgtggtc atgctcatac aggccgcgaa cgtcattacc cgccgcaagc cattcagtaa
    13861 acgccccccg ctggaatttt tcgtaaaact caccccataa cagttctgaa agcttatccc
    13921 agcgaacaac gtagcccgtc agcgtaccgg ctccgctggt ggtgatttcc gatgaccgga
    13981 tttccatact cttcataatt ttttcaccca taaaacacta aaggggcttt ttagcccctt
    14041 ctgtatgctg ttaatcgtcg tcttccggca gtgccagaat cttgatcgcg ttcgaatcca
    14101 ccacgccacc gcctaaatat ttttgcgtga aaattttgat gaatcctggt tctgtcaggt
    14161 tgtccggtct ggtgcgaaca cctgtttcgt gatcaacaat gtagtaaccg cgtttgaagt
    14221 cacccagggc aataacgtta tcaggcataa actccagata ttcgaccgga aggcccagca
    14281 acgtatcagg atcacccgcc tgtaaacggt cgcgccagat ataatcaccg ttcgcgttct
    14341 tcaccttctg aagttttgcc gccgtcgtgg agttaaccac tcagaccgcg tttttgcggt
    14401 attttttacg taatgcaaat ttcaggtcga tcagcgggtc cgcagatgtc cacgccagag
    14461 attcggaagg tttaattacc tgcaacgtac caaaatcacg ctctttgtcg ttcttctctg
    14521 cacggggtac ggataaaaaa ccttttgctt ttttgtcacc gtcgcccaca accagatcgc
    14581 tttcttcggt ttccgtgaag gtgtcgccaa tctcacccgt cagccatgaa aggatgtcca
    14641 catcggaaaa atccacgatt tcctgtgtgg tgcgcgggta cgcatagacc ggatacagct
    14701 taatgctcac ctcgttaatc tgcggggtgc tggtctgttc gcgtgcctta ccctcttcac
    14761 cgtggttaac ggtcgcaccg ccagcggaaa caagctgctt aaactcgttg ctgctgattt
    14821 tcttcacggt acagatgcga cgcatggtgg attcatccgc cagcattcgc atgatttcgg
    14881 tgttcagttc agggataacg gtataaccac catcagcggg aacgcctgta cttaatgcgc
    14941 gggtttcacc ggtcagaatg tagttacgta gttctgcggg gtcagtggtc tggctgcttt
    15001 tacctggctt gctgcgctct tcgtctgcaa tggcttcaag gcgggaaatg tctttatcga
    15061 gggattcagc tttagcgcgt aattcgtcaa attttgcgcc ctcagcatcg ttaagactgc
    15121 ggttttcttt ttcagcgttc tccagcatgt cgcgcatctg atttttaatg gcggttttct
    15181 gctggcgtaa ttcgattatt ctctgcataa aaaaagtcct ggggttaagt aaggaactcc
    15241 aggacgcggc aaacactcaa ccgtttttca taaggaaatc agtaatcgca ccgatcgttt
    15301 tcccgcctgg taatgaatat tggcgagcac attaacaggc gggaaagtgg ccccagcgtc
    15361 ctggcaccac gggcgagaat aatcatgatt cagttcgggt aaaatatgcc gatccgctca
    15421 gtgaacaacg tggaacaacc acgaacaaat aatttacaaa aaataacaaa aagccggatt
    15481 gctccggctg tatgtgctgt atgggtgctc agtctctgat ccctttcaga aattctatca
    15541 atgcatctgt ctgttcagga tttactgcca gcatttcacc ggatagcgcg caacgaacaa
    15601 aaccatgatg atccttttca atcagcgccc ccgtttccag aaatgcgcgg taatcagtga
    15661 tattcatcgt ctccatgttg tcagcatggt atttatcacg ctgtttaagt atctcatcaa
    15721 atttcatcgg cattgcgttt tcctctgttg tgtctgtttg tttcagatag taactatgcc
    15781 ggaccgtgac gaaaagccag tagtgctcca taccgtttca aatgacgcaa aaaaagccgg
    15841 attgctccgg ctgcgtggtg tggttctgat attcctactc tgctaatttg ctcagcccca
    15901 tgtctgcgta agtgcgattt actgcatttc tcaggtctgc gtagttctcc gttggcggct
    15961 ccggtgtgcc cttgctgctc tgttttacca gcgggtcgcc caccagctgc ccgtaagctg
    16021 caatttgtgc tgtcatattg ccacctcagt gaagcgatcc ggcattgttc tttttcatct
    16081 cttccatcat tttttcagtg agcattacgc actgaaaccc catgtttttg atgtctacgg
    16141 gtttcatgcc tttgtcagtg gatatcacag gcagaatatc ctgatacaca tcgcggtcag
    16201 ggtaactaag cacacaaagc tcccatgatg ccacgggtga ggatccttcg tctgtttcaa
    16261 cccaccagcc tggtgtagct gcgcttattt gcagaatcgt gatccgtccg ttgtgcttgt
    16321 aaaatttttc accaaatttt ggttcgctca tgattccacc tctccggttt taacgttgat
    16381 tgttgttacc tgttccgctt cggcaatctc ccgctctgtc agcgtggcaa aatttgccgc
    16441 cgtcgtggtc atgaatgcgc ttatcagttc gggatgtgct ttcgcgtatc cttcccccgc
    16501 gtggcggtct atcgttctga ttgccacctt taaagcgtgc tctgtcatgt ctaacgcgcg
    16561 atatttcggt tctgttctgt ctctgcgttt tttgaatgat ttattaaatt tccctgaagt
    16621 gtgcatattt atttttaccc cctcgtttaa aaagttttga gttgtgcctc cccttgtcta
    16681 ccttatctac cttagtggcc ctcatgccag taatggcgct gctttcagcg gggtagagtg
    16741 cttttatcca ctatctaccc cgtgtctacc tccctgtctg attcaggtaa aatcaggtag
    16801 agagggtaga tagtgggtag acagtaaaaa aaggctatct acctaactta atgcactgaa
    16861 ttaaatgtat ttttctttac tcaggtagac agggtagaca gcaattacaa aaaattataa
    16921 aaacgcgtcg cactcgtctg ttgttattgc gttagtctgc gttactccct taactttccg
    16981 cgtaatatat tcatgtccgt aaactttcgc ggctggcttc atagccttgc caaagtcatt
    17041 tacgtttagc ggtttgctcc tgcctgcgta cgccataaac gccagataga cgcggtaaag
    17101 gctgtttctg gtcgtgtact tcactgaatc gccaccgccg cccatcatca ggccgcgtgc
    17161 ttcctccaga aaattcagga actggcaaaa ctcaataacc ggatccgtct gttgctttat
    17221 tgccagagct tcatcaccgt cacgctgttc cagtagtaaa gcccgtgctt tctcagagtc
    17281 ggtaaagttc gccagcaatc ggcggataat gacagggatt tcagccgcaa tcttttccgg
    17341 tagctccctg tctttttcgg cctcactaac gatattgtcg aaacggaaaa tcacgcgacg
    17401 acgtgccaca cctccggccc gttcggtgaa tatcatcggg ttattgttgg tcgccagcac
    17461 caccgccctg attacagccg tgaaacgctt ttcgtatttc gggttaattt ccacggggtc
    17521 accgcccgtg attttcttga tgcccgttcc ttcgcctgta tatttcggct ggtcagccag
    17581 gacgataaga cgactcccga caacctgcgc acgtccacca gcatcatcaa gcgatgtcat
    17641 ttcagcgctt accgtgttct gtttccctgc cagaaggctg gctatgtgtg tgaatgtact
    17701 tttaccgctc ccgccgtctc cggtggcctc aataaacatc tgccagtcgt accggttcgc
    17761 cataatcatg tacagcgcgg cacatatacg catcatcttg cgctggtctt ttccggctgc
    17821 gtgctcaagc catttatgaa agtttagcgc gttatcgcgg atgttctccc ctggtgctgg
    17881 tggcgtgtac tcaatgccgt tgtgcgtggt gatccagttc tccggcgtgt gcggggaaaa
    17941 ttcccccgtt ttcaggtcaa gcgcaccatt ggcgaacggc agcaaatcgc cggacggctc
    18001 gcccattggt tcggcaataa cttttaacgc ttccacggcg ttattgatta cgcgcttgct
    18061 gaaagtggcc ctgtgctctg aatagatcac caccatttcg cggctaagtt ccattgtgct
    18121 gaccggacac cataccccgc cgcgccatac gtgaacgatt tcactttcct gatgcacaca
    18181 aacgccatca aagcgctcgg caagcagctg cgcgcgctca ctgtctgcca tttgtgccag
    18241 ttgcgtattt ttctgctccg gcatggtaag ccctgcggca atattttcac gctcagcatt
    18301 cagatagcgc cgccagtttt cacacttctg gacgtgcatt ccttcggggt aaaaatttgc
    18361 gtcctgaata tctgccgccg ccagcttctg accaatcttt ttggtctcca ccaaatccag
    18421 ttctccggcc tggtacagcc ttacgcgttt ttttccatcc ggaacaattt tcagtgcatc
    18481 aagttcggca agctgatttg gcccaagcca cacaggcggc acattatcgc cggatgcggg
    18541 gccatcctgc tcctgccatt gtttcgcgtg cgaccaggca tcactacccg caaagataat
    18601 tacctctgtg tctttgtgtt ttattccgcg tggctgtttt tttacgttcg gtgccagttt
    18661 cattttttac ccctgaatac gttaagcatc ttttttattt cctgaatatt ggcgcgtgct
    18721 ttctccctgc tggttggttt actgcggggc gctgcctgta ccagcgtgaa atcacgccgg
    18781 aattgataaa caggcatcac gcaatcatat tcgtaacctt cacggcggta ggtgatgcgc
    18841 cgttctgcca cgcctttaat cgttaccatg ccgccgtact ggtcgcggta aatatcaccg
    18901 cgcgtaaatt tagggtgagt gttgccactg gcagttaagc cagaatattt aagtttcatt
    18961 atttttattc tccggtgtgt tgttctttat atctgtcgtg caatagatct atttcttgca
    19021 gttccattat tacaggctca agaagcgtta ttaatgccgt ggcaattctt gatttttgtt
    19081 tgtcgcgttc attgtcgcca agtgtttcaa gccatatgcg caatatttcc agcatgtttt
    19141 cactgtgaga aagtgcaaga aatgcgcggt ctattgtttc gtggtaaata tcacgcatat
    19201 taatccccgt ccgtcgtttt tcttaaaata acctctgtca cgaaatcagc ataatcggcg
    19261 gcgatattca gtacatcaag cccgttgatt tatattctct cgtggaaagt aagaaaaaag
    19321 ccgctctaat aagttctggc attgacgaaa gcgcatcagc cgcatcatca ggaacgccgg
    19381 aaaattcctg tttcagggaa ttaaaacgat catcacgcat aacccccccc attttcacaa
    19441 tcggcaacaa gaatattttt agcgtcattc agtgaccgcg ttgcgatgta tcggatacat
    19501 tccagggcga actgtgtccg gtctttctgt tcttctccct gcgcaagctc tgccgtgcat
    19561 tcaatatcaa taagcgcgtg catcagcgta gtgagtgcgg cggctgctgc gttcggtgtg
    19621 gttttattgt gcataggttt ccccctgacg aatacgggcg gcgaatacag caacacaacc
    19681 ggacgggcaa cggctacgcg cttcgcgttc cgtccaagcg gttacgtgga tgatttggga
    19741 ttctccggca ctcagtgcca gaaaacgcca cacaaaggcc gtttgtgtgt gtacaaggtg
    19801 tggtatatga tttacggcaa ccataacggc tcctagttta cgttgttggt tagaagcccc
    19861 gttactgctc ctacagtgcg gggctttgct ttttgtgttg attgtgtgta atgtgtcact
    19921 acacataaat acattacctg aggtgtaatt aacgtgtcaa cacacaaaaa cgaaaggcgt
    19981 ggtaatcctc cgttccagtt ccgtttagat cctgacttac gggaaaagat ggaaaaggca
    20041 caacaacagg acggcgatga ttctcttgct gcctggataa agcgaataat ccgcaaagaa
    20101 ttacagcaac gcggcataga acccaaagga taaaaactat cagcgccgtg gtgtgaggta
    20161 ctacggcgca ttgctttaca ggcgcacaca atgaccaacg aagaatcaac cgaaaaaaca
    20221 tcacctgcac gaaaaagacg acgcagaaag aaagagcatg aatcagaacg attcgcgccg
    20281 tgttcttttg cccttgagaa gtaccttaaa gaatactcat caaaggagag aacttcgcag
    20341 ttatggcaac gcactgaacc agactgattg cattgcccac cagcctgata gcggctatca
    20401 tccccgtgct tatgtttggg atcacacaca caaacggcgc agcgggttat ctgttcagaa
    20461 aggcggctcc atttcggggc cgcttttttt atgcctgaaa aaccccaatt ttgtggtttt
    20521 ccagtttcac cagggcgaac gaatccccgc ccacgttctg gcgtatattc aatcttcatg
    20581 gttatagctc tgtgttcaga tgattgatgt gtggcggctg cgtgccgcca gcgtgattaa
    20641 tgaactgcct tgcagctatc cttccaggcc agaacctcgg ataaagacca gccaacggaa
    20701 cgaccgccaa gtttacgacg tgatgggaat tgtccggcct tttccaggcg gtagcggcat
    20761 gagcggctaa ggcctgttag cttttcgcat tctttttcac gtataaaccg atcagtgctt
    20821 aacactattg ccccctttcg tttcttaaag agttatttcg tgttctattg cgttgggatg
    20881 tgtctgattg tgtcaggatg attcagagtt ggcaaatgtt gaggccgtat ggtttacaga
    20941 aagaggaata atcaggataa aatcctttaa attcatgtta atacaaaggc ataaaatatt
    21001 gtttcatgcc ttttttctcg ctatttaaag agtgattcgc tagtgtataa aaaatcagta
    21061 acacattaaa aatcagctac ttataaatct gtacactttt tcacctcttg ttcgtagttg
    21121 ttccgcattg ttgctcattg ttgcacgttg tatctgttcg catatccagt atgcgcatac
    21181 tgaaaaaaca cgaaaaaaat tattttcttc tggctactgg tagcgtggtt acgttttcat
    21241 gtgttcccgc cagtatccct aaccgctccg tccacatatc cagcgcattg cgtttagcat
    21301 ccagataacg ggaatgatta taaactcgct gcattcctgg catctggtgg cctgtaagct
    21361 gctccacgac gtgaggatcc acgcctaaat cgttcagcat cgttgtaaag gtgcgccgga
    21421 tgtcatgtag tgaccagtga gggtgattta gcctcctgtg cgctaatctg ccgtactgcg
    21481 acacgcttgt ttcctgtttc acttccccca gcaataagcc cgtgtgcctg ttctgctcca
    21541 ccagctgcgt gacgaacggc agtattgctt ccggtatggg ccggaatatt gccaccttcg
    21601 ttttgctgtg ttctttcggc acggtccaga gcatttcggt aaaatcccac tcgctgatct
    21661 ccgataacct cagttctacc gtccggcatc cgaacacaat caggaggcgg attaacgcga
    21721 tgtagtaggg ggagaatatt tttttgtcca gtgcctgcaa taattcgccc agttctttgg
    21781 tgcttaagac acgctcgctt atatccggtt ttttcccaac gtccgccaca ctcatatcat
    21841 caagaacgtt gctgattgca tagcgccgcc tccggcagaa cttaagcgcc tgtttgcacg
    21901 tctgtagcaa gaatccggca gtaacaggcg ttcgctttgc cacctggtca aaacaggcca
    21961 gccagtgccg tagctcgcat ttatccagcg gcatagcacc aatgtgctgt attacgtgat
    22021 tattaaggcg ctttttcagg gcggcataat ccacgcggtt ttcctttgcg tacgactcaa
    22081 gccagtaggt gagcgcatcg ccaaccgtta ccggctttaa cgcttcctgt acggtgtaat
    22141 taagctcatg acgtggattt ttcccctctg ccagccatgc gcgacactgg gcggcttttt
    22201 ccctggctga tttcaggctc agatcaggat aatttcccag cttaatgcgt tccggtggtg
    22261 ccccccttcc cgttccggcc ctgtaagtga aataccaggt taaaaggcca ctggttgaat
    22321 gcctgacgct caggtttcca ccgtcattaa gaaaggctgt tttttgggcg ggtgtgccgt
    22381 tgattttcct cagctgtgta tcgctcagtt tgttaagtgc tctgctcata attttgattc
    22441 cggtcatcac aattaactac accattaact acaccgatcg ttgcacaaag ggctacaacg
    22501 tgaaacaagc tggaacaagg aaaatcacaa atctgtttat aatcaaaaac ataatgaaca
    22561 atctgaaaca ttatgaaacg gcaaaaaaca ctaattgata aaatatgtcc atacttcacg
    22621 cattacgtta agcatccgtt ataatcggtt gcagatacca gcctgtggat gcttaacatg
    22681 gaataccaac tcactcttaa ctggcccgat tttcttgaac gtcactggca gaaacgcccg
    22741 gtggtgttaa aacgcggctt taataatttt attgacccga tctctccaga cgagttggcg
    22801 ggtctggcga tggaaagcga agttgacagt cgactggtca gtcaccagga tggcaaatgg
    22861 caggtcagcc acggcccgtt cgaaagctac gatcatctcg gtgaaaccaa ctggtcatta
    22921 ctggtacagg cagtaaacca ctggcatgag ccgaccgccg cgctgatgcg accgttccgt
    22981 gaactaccgg actggcgtat tgatgatctg atgatttctt tttctgtacc tggcggcggc
    23041 gtcggcccgc atctcgatca gtacgacgtg tttatcattc agggtaccgg acgtcgtcgc
    23101 tggcgagtgg gcgaaaagct gcaaatgaaa cagcactgcc cacatccgga tctgttacag
    23161 gtcgatccgt tcgaagccat catcgatgaa gagctggagc ctggtgatat tctttatatt
    23221 ccgccaggat tcccgcatga aggctacgcg ctggaaaatg cgatgaacta ttccgtgggt
    23281 tttcgcgcgc caaatacgcg ggaattaatt agcggatttg ccgattatgt gctgcaacgt
    23341 gaactgggcg gcaactacta cagcgatccg gatgttccac ctcgtgctca tcctgcggat
    23401 gttctgccgc aagagatgga taaactgcgt gagatgatgc tcgaattgat caaccagccg
    23461 gaacacttta agcaatggtt tggcgagttt atatcccagt cacgtcatga actggatatc
    23521 gcgccgccgg aaccgcctta tcagccagat gaaatctacg atgcgctgaa acaaggtgat
    23581 gtgctggtgc gcctgggtgg tctgcgcgta ttgcgcattg gcgacgacat gtatgccaat
    23641 ggtgagaaga tcgattcccc gcaccgtccg gcactggatg cactcgccag caacattgcg
    23701 ctgactgcgg agaattttgg cgatgcgctg gaagatccgt cattcctcgc gatgctcgcg
    23761 gcgctggtca atagcgggta ttggttcttc gaagggtaag tttggattta agccggatgc
    23821 agcatcacat gccgcatccc ttttcgctta cttccgttgc gccgttaact cggcaatacg
    23881 gacgatcacc tgcaccgctt tttccatacc ttccagaatc acaaactcat gcttaccatg
    23941 atagttgtaa ccgccagtga acaggttcgg gcaaggtaat cccataaacg acaactgcgc
    24001 gccgtcggta ccgccgcgga tcggtttcag ttccggttca atatcgcagt cacgcatcgc
    24061 ctgctgggcg atatcgagaa tatgcggatg ctcaaccact ttctcgcgca tattgtagta
    24121 actgtcttca atcaccaatt caatgtagca atcaggatgt aaccctttgc ccactttttt
    24181 ggcgatctcc atcattttac gtttacgcgc ttcaaactgt ttacggtcga aatcacggat
    24241 gatgtagtgc atatcggccc gttcaacggt gcctttcatg ctcgccaggt gatagaaacc
    24301 ttcatagcct tctgtcattt ccgggctttc atccgccgga acttccgcat gaatacgtgc
    24361 cgccagcgac agcgcattta ccatcactcc tttcgccgtg cccggatgaa cattgttacc
    24421 gacaattttt atattgaccg atgcggcgtt gaagttttca aactccagtt cgcctacgcc
    24481 accaccgtca acagtgtaag cccagcgggc atcgaaggca tcaacatcaa aatgtttcgc
    24541 ccctttgccc acttcttcat ccggggtaaa ggcgacgcga atatcaccat gcggaatgtt
    24601 tttctgttgc aataccgcca gcgcggtcat gatttctgca atacctgctt tgtcatcagc
    24661 acctaacaag gttttaccat cggtggtaat caacgtctga cccagtagct gatgcagcac
    24721 cgggaacata accggtgata aaacttcatc gccgataccc agcgcaatat cgccaccgcg
    24781 atagttttca acaatttgcg gattaacatt tttgccgctg caatccggtg aggtatccac
    24841 atgagaaata aagccaatcg ccgggatatc gccagggacg ttagccggta acgtcgccat
    24901 caaagtgccc ttctcactta aggtcacatt gataagcccc atttcttcga gctgctcttt
    24961 cagcagatgc aataacttcc attggccttc cgtgctggga acctgtctca cccctgcttt
    25021 tgattgggta tccagagaca cgtagttcaa aaatcgctca agtagtttat ccatgtagtc
    25081 accctcactt tttgtgacaa cattattaag aagcaagaaa agacaaatat tgcgtcaggt
    25141 cacttttacc cctgcaagcg ggaatattta tcagcattac cttaatgaat ataaagctaa
    25201 gccagtaatt cacaacaaag attggcgatt caagcggttt gccgtagaat taccgccctc
    25261 attaggagtc accaaggtgg ttaaccacaa accccgcatc ggtaagccat ccgttgcgtt
    25321 tacatgggac agagtaaaaa attgaataaa caaccgagtt cgctttcacc gctggtgcaa
    25381 ttggcgggaa ttcgcaaatg ctttgatggt aaagaggtca ttccccagct ggatctgact
    25441 atcaacaatg gcgagttcct cacgctgctt ggcccttctg gctgcggtaa aacaaccgtt
    25501 cttcgcctga ttgcaggtct ggaaactgtt gattccggac gcatcatgct ggataacgag
    25561 gacatcaccc acgttccggc ggaaaaccgc tatgtgaaca ctgttttcca aagctatgca
    25621 cttttccccc acatgaccgt gttcgaaaat gtggcctttg ggttgcgcat gcaaaaaacc
    25681 cccgctgctg aaattacgcc ccgcgtgatg gaagccctgc ggatggtgca gctggaaacc
    25741 ttcgctcaac gtaaaccgca tcagctctct ggtggtcaac agcaacgcgt cgccattgct
    25801 cgagcggtgg ttaacaagcc tcgtctgttg ttgctggatg agtcgctctc agcgctggat
    25861 tacaaactgc gtaagcaaat gcagaacgag ctgaaagcgt tacagcgtaa gcttggcatt
    25921 acattcgttt tcgtgactca cgaccaggaa gaagcactca ccatgtcgga caggattgtg
    25981 gtgatgcgcg atggtcgcat tgagcaagac ggcacgccgc gtgaaatcta cgaagagccg
    26041 aaaaacctgt ttgttgccgg gttcattggc gaaattaata tgtttaacgc cacggtgatc
    26101 gagcgactcg acgagcagcg cgtacgcgcc aacgttgaag gccgcgaatg taatatctac
    26161 gttaacttcg ccgttgaacc ggggcaaaaa ctgcatgttc tgctgcgccc ggaagactta
    26221 cgtgttgaag agattaacga cgacaaccac gctgaagggc tgattggtta cgttcgtgag
    26281 cgtaactaca aaggcatgac gctggagtcg gttgttgaac tggaaaatgg caagatggtg
    26341 atggtcagcg aattcttcaa tgaagacgat cctgactttg accactctct cgaccaaaaa
    26401 atggccatta attgggtaga aagctgggag gtcgtactgg ctgatgaaga acacaagtaa
    26461 gttccagaat gtagtgattg tcactattgt cggttggctt gtgttgtttg tctttctgcc
    26521 caacctgatg atcattggca ccagcttttt gacccgcgac gacgccagtt tcgtcaaaat
    26581 ggtctttaca ctggataact acacgcgtct gctcgatccg ctctattttg aagtgctatt
    26641 gcactcgctg aatatggcgc tgatcgccac cctcgcctgc ctggtgctgg gctatccatt
    26701 tgcctggttt ctggcgaagc tgccacacaa ggtgcgtccg ctgctgctgt ttctgctgat
    26761 tgttccgttc tggaccaact cattgattcg tatctacggg ctgaaaattt tcctcagcac
    26821 caaaggctat ctcaacgagt ttttgctctg gctgggcgtt atcgacacac caatccgtat
    26881 catgttcacg cccagtgcgg tgattatcgg tctggtttac attctgctgc cgtttatggt
    26941 gatgccgctg tactccagta tcgaaaaact ggataagccg ttgcttgagg cggcgcgcga
    27001 tctcggtgcc agcaagttac agacttttat ccgcatcatt attccactga cgatgccggg
    27061 gattattgcc ggatgtctgc tggtgatgct gccagcgatg ggcctgttct atgtgtccga
    27121 cctgatgggc ggtgcgaaaa acctgctgat cggtaacgtc atcaaggtac agttcctcaa
    27181 tattcgtgac tggccgtttg gtgcagccac cagcattacg ctgactatcg taatgggcct
    27241 gatgttgctg gtttactggc gcgcttctcg tttgctgaat aagaaggtgg aactcgaatg
    27301 atcggtcgac tgcttcgcgg cggttttatg accgctatct acgcgtacct gtatatccca
    27361 atcattattt tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa ctggcagggt
    27421 tttaccacca aatggtatag cctgctgatg aacaacgaca gcctgttaca ggcagcgcag
    27481 cattcactaa caatggcggt gttttcggcg acgtttgcta cgcttatcgg ttcactgacg
    27541 gcagttgcgc tgtaccgtta tcgctttcgc ggtaagccgt tcgttagcgg aatgctgttt
    27601 gtggtaatga tgtcaccaga tatcgtgatg gcaatttcgc tgctggtgct gtttatgctg
    27661 ctgggtattc agcttggctt ctggtcgctg ctgttctcgc atatcacctt ctgcctgcca
    27721 tttgtggtgg tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat gctggaagcg
    27781 gcgaaagatc tcggtgccag cgaatttacc attctgcgaa aaatcattct gccactggca
    27841 atgccagcgg tggcggcggg ctgggtgtta agctttaccc tgtcgatgga cgatgtggta
    27901 gtttcttcat tcgtcaccgg accaagttat gaaattctgc cgttaaaaat ttattcgatg
    27961 gtcaaagtcg gcgtatcgcc ggaagttaac gcgctggcaa ccatattact ggtgctgtcg
    28021 ctggtgatgg ttattgccag ccagcttatt gctcgtgata aaacgaaagg taacacaggg
    28081 gacgttaaat gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg gcactgggca
    28141 tgagcgccgc tcacgccgat gacaacaaca cgctgtattt ctacaactgg accgagtacg
    28201 tgccgccagg actgcttgaa cagttcacca aagaaaccgg tattaaggtt atctattcga
    28261 cttacgagtc gaacgaaacc atgtacgcca agctgaaaac gtacaaagac ggtgcctatg
    28321 acctggtggt tccttcaacc tattacgtcg ataaaatgcg taaagagggg atgatccaga
    28381 agatcgacaa gtcgaagtta acgaacttca gcaatctcga tccagacatg ctcaacaagc
    28441 cttttgaccc gaataacgac tactccattc cgtatatctg gggtgcgacg gcgattggtg
    28501 ttaacggtga tgcggtggat ccgaaatctg tcaccagctg ggccgatctg tggaagccag
    28561 agtacaaagg cagcctgctg ttgaccgacg atgcccgtga agtgttccag atggcgctgc
    28621 gtaagctggg ctactccggt aacaccaccg atccgaaaga gattgaagct gcatataacg
    28681 agctgaaaaa actgatgcca aacgtcgcag cgtttaactc cgataacccg gctaacccgt
    28741 acatggaagg cgaagttaac ctcggcatga tctggaacgg ttctgctttc gttgcacgcc
    28801 aggcgggtac gcctattgac gtggtgtggc cgaaagaagg cggcatattc tggatggaca
    28861 gcctggcgat cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc
    28921 tgctgcgccc ggatgtggca aaacaggttg ctgaaactat cggttatcca acgccaaacc
    28981 ttgcggcgcg taagctgtta agtccagaag tggcaaacga taagacgctc tacccggatg
    29041 ctgaaaccat taaaaatggc gaatggcaga atgacgttgg cgcagccagc agcatttatg
    29101 aagagtatta tcagaagctg aaagcaggac gttaattcag caagcgggcg gtggtattac
    29161 cgcccgtttt ttagttagca aggatgtggc taatgagtca ggacagtaaa gttttatccc
    29221 gctctttcgt cggtattgtg gtccgcttgt aggcctgata agcgtagcgc atcaggcaat
    29281 gcttcctgct ttataatccc tccagcaact tttcaacaaa ttctggcacc acctggcttg
    29341 ccgggccgta atatttctcg gcaaattcat taccaacctg actcggttca agattcagtt
    29401 caacggtgtg agcaccatgt agtttggctt cgtgtacaaa cccagccgcc ggatagacat
    29461 gcccggaagt accaatggca ataaagacat ccgccatcga caacgccata taaatttcat
    29521 ccatgccgag tggcatttcg ccaaaccaaa cgacgtgcgg gcgcaagggt gccgggaact
    29581 ggcaacaatg gcatttatct tctggcgtaa cgtctccggt ccagtcgaga acctgaccac
    29641 tttgcgaaca acgaactttc agcaattccc catgcatatg aatgacattg gtattacctg
    29701 cgcgttcatg caggttgtct atattctgcg ttaccagcaa aaagcgatcg ccgagagcgt
    29761 cttgcagttt agccagcgca agatgcgcgg cgttaggctg aatttctggc tgctgcagct
    29821 gtcgacgacg ggcgttataa aacgcttgca ccagttcagg atcgcgatcg aaaccttccg
    29881 gcgttgccac atcttcaacc cgatgttctt cccacaggcc atctgcggcg cgaaaggtac
    29941 gaatacctga ttccgcagaa attcctgccc ctgtcagtac gagtactctt ggtttttcca
    30001 ttgcttccgg caccacttta tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc
    30061 gacgtttatt tttacgaaaa cgacttaacc gatgaccccg acgcgacagc atagcaacct
    30121 ctgttgttta atcggttaga tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt
    30181 ggcgcgcgcg ttcaatgcgt ggaacacgag ctacaggtaa gagatgacaa ggcaacctgt
    30241 ccgccagttg cgttgtgatt gccgggaaat tcgataagcc accaccaatg acgaccaggt
    30301 cagggtcaac aatggtcagg atatttccca gagaaaccgc taataaatcc agataacgct
    30361 caacgtgcgc ccttgcctgc tcatcgcctt gatcataaag cgcaataatt tcgggagcct
    30421 gcaacggttg atgataatag tgttgataca gccacgcaaa accgcgacca gacagataat
    30481 tttcaatgca gccatgctga ccacagccgc agcggcgtaa cgggaaatcc aaccccatca
    30541 tggttaacgc atcaaccggc agacgcatat ggccaaactc gccggtaatg tagcttttac
    30601 cagtaatcgg tttgccgttg aaaaccagcc cgccgccaac gccggtgccg agaattaacc
    30661 ccatcaccag tggatattgc gtaaattcat catcccaggc ttctgaaagg gcaaaacagt
    30721 tggcatcgtt atcaaggcgt acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg
    30781 gtttgccgct ggcagcgggg acattagcgg catacagctt accatcttct gtttccggca
    30841 ttcccggaat accgatgccg acagaacctt tacagccaaa acgttggtca gcttcagcta
    30901 ccagctcaca cactgcatct aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct
    30961 tttcccactg caactgccga ccgctatcaa acacgccaag cgcaattttt gttccaccaa
    31021 tatcaaaccc gtaatacatt gccgctcctt gttttaatgt actgccttta ctggccgcta
    31081 aggactcgcg cagggtcaat attactggcg cgccgcgccg gataccaact tgccaaaaga
    31141 ctcagcaaca atgctgtgac cagtacgtag aagacgtcca gccagtgcaa ttccgatggc
    31201 aggaagtcaa taaaataaat atcgctggag aggaactgat gaccgatcag cttttcaatc
    31261 cactcaataa tcggggtaag ttgcagtgaa acaactacgc cgataaccac cccacacagg
    31321 ctgccgaaca gccctgccag caatccatac cagacaaaga tggcgcgaat taaaccatct
    31381 ttcgccccca gcgttcttaa tactgcgata tcgccactct tgtctttcac cgccatcact
    31441 aaggtggaga cgatgttgaa acaggccacg ccaatcacca gtaccatcgc cagatacata
    31501 atggcgcgga tcatctggat atcgcgatac atatagccgt aagtaccaat ccaactttta
    31561 atataaacat agctgttggt cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa
    31621 acatccgtca ttttaagggc aatacctgac acgctggaac ccatatcaag atattgttgg
    31681 gcatccgcca gcgggatcat ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata
    31741 ccggcaatgt gcaaacgcac acgttttggc tgcatcagtt tatgctcggg attcgagttg
    31801 gggatcatga tcgacaccca atcgccctgc ttcactttca gcgcatccgc cacgcctttg
    31861 ccgatgataa tttgctgttc gcccgcttta aaattgcgcc acgcatcccc ctgaacaaac
    31921 gagggtaatg cgctcagacg ctgttcctgt tgtgggttaa cgcccttcac ctggattgcg
    31981 cgcagattcg ctccactttc caccagaccg gtgaaattga tatacggcgc agcggcggca
    32041 atacccggca ctttctgcac gttatccagt gcttcctgcc agttagtcca tggctgattt
    32101 accgcttcga tttcgccatg cggcaccacc gccagaatgc ggttattcag ttcgcgttca
    32161 aagccgttca tcgcgcttaa gccgacgatc aataccgcca cgccgagggc aatgccaatg
    32221 gtagaaatga cagagattag cgacaccatg ccgccgcgtc gccgtccgcg actaaaacgc
    32281 aggccaatca ataacgataa aggcatcgcc attactccgc ccccatcagg ctcagttccg
    32341 ccgtcagacg tccatcacgc atttccagtt ggcggctcat acgtttcgcc agttgcaggt
    32401 cgtgagtgac caccaggaaa gcggtgccct gcaagcgatt caactcccca agcaactgga
    32461 agatgctgtc ggcgttacgc gcatcgaggt taccggtagg ttcatccgcc agtaccagac
    32521 gcggattatt gaccagcgca cgggcaatcg ctacacgctg gcgttcgccg ccagaaagtt
    32581 cagatgggcg gtgattcgca cgatgctcca gccccaccgc ttttaacatc tccagtgcac
    32641 agctgttgat ttcagcgggc tttttcttgc caatcagcag tggcatagcc acgttttcca
    32701 gggcagtaaa atccggcagc aggtggtgaa actgataaat aaagcccagc ttctggttgc
    32761 gcagttctgc tttcgccgct gaagacagtt tgctcatcgg ctgaccgtta aagatcacat
    32821 cgccggaggt tggcgtatcc agcccgccta acaggtgcag caaggtactt ttaccggaac
    32881 cagagctacc gacaattgcc atcatttcac cttcgcccac gctgaaactg acattgagca
    32941 gcacatcggt ttgcacactg ccttcctgat agcgtttgca caggttgtcg cattgcaaca
    33001 ggatcttatt cataacgtaa agcctcagcg ggttgtgtgg cggcagcgcg ccatgaaggg
    33061 taaagcgtag acagcagcgc gatagccatc gccaccagcg caataacaat gacctgtaaa
    33121 ggttcgatag ccaccggcag cgccgcgcca tcaagcagga cgccgattat cggcatcaga
    33181 ttatttaact ggctggcaag cagtgcgcca agcgccgctc cgaggattgc accgataatc
    33241 ccggcgctag ccccttgcac cataaagacc atcatgattt gtcgcggagt taagccttgc
    33301 gtttgcagga tcgcgacttc gccctgcttc tccatcacca tcagccccag cgaggtaata
    33361 atgttaaacg ccgcaacagc gacaatcagg ctcagcagta agcccatcat atttttttcc
    33421 atgcgtacgg cctggaacag ctcgccttta cggtcacgcc agtcctgcca tttgctgcct
    33481 tcaggcagtt tttgctgact taaagagtca actctcagcg gctcatccag ccacaaacgc
    33541 cagccggtaa tattggctgc cggataacgc atcagacgcg aggcatcctc aatattcacc
    33601 agcatttcat agccatcgac ttcactgttg gcggcgaaag tgccaatcac attgaacagg
    33661 cgctgacttg gaatacgccc catcggcgtg aactggctgg cagatggcac catcacgcgg
    33721 atttgatcac cgcgattgac gcccaactgt gaggcaagtt gttcgccgag gatgacatta
    33781 tatttccccg gctcgaggtc agtttgtttc acattgacca gatacggcgt tagtggatct
    33841 ttttgcgccg ggtcgatacc gagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt
    33901 accacatcac cggtagtaat aggtgcgacg cgattaacgc cgtccagttt gactgccgtt
    33961 tccgggagtt gctgcgggtt aagagagcca tgctcagaag agagaattgc ctgtggcatc
    34021 aggccaagga tgttgttttg cagctcgcgc tcaaagccgt tcatcactga caatactgtg
    34081 accagcgcca tcaccccgag ggtaatgccg atggtagaaa gccaggagac gaaacgaccg
    34141 aagcgatcca ctgcacgccc acgcatgtaa cgcaggccaa taaatagagc gacaggttgg
    34201 tacatgaaat ccgtctggtt gctgtagcaa agacacgagt atataagcga aagcaagatg
    34261 ggtaaacgtc tgaactgtaa gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa
    34321 tcggctaaat tgccatattt tatcatcaat cttgccgcct gatcttaatc tgaaaaatca
    34381 tccaatacag acagataccc attacacctc gggtcataac cagcaggatc aaccgcatgt
    34441 aaattaaaaa agagcgtgtt gttctgatga aacaaaaaga gctatggatt aaccagatca
    34501 aagggttatg tatttgtctg gtggtgattt atcactcggt cattaccttt tatccgcata
    34561 tgaccacttt tcagcatccg ttatcggaag tcctgagcaa atgctggatc tatttcaatc
    34621 tttaccttgc cccctttcgt atgccggttt ttttctttat atccggctat ttgattcgcc
    34681 gctatatcga cagcgtaccg tggggaaatt gtctcgataa acggatctgg aacatcttct
    34741 gggtgctggc gctttggggc gtggtgcagt ggctggcgtt aagtgcgctg aaccagtggc
    34801 tggcacctga gcgcgattta agcaatgcct ccaatgccgc ttatgccgat tccaccggtg
    34861 agttcctgca cgggatgatc accgccagca ccagcttgtg gtatctgtat gcgttaattg
    34921 tctatttcgt ggtatgtaaa atttttagcc gtctggcgct gccactattc gccttgtttg
    34981 tactgctgag tgtggcggtt aatttcgttc ccacgccgtg gtggggaatg aacagtgtga
    35041 tacgcaattt gccttattac agccttggcg catggtttgg cgcaacaata atgacctgtg
    35101 ttaaagaggt gccgttgcgc cgccatctgc tgatggcttc tttgcttacc gttctggcgg
    35161 tcggtgcctg gttgtttact atctcgctgc tgttgtcgct ggtatcgatt gtggtgatca
    35221 tgaagctgtt ttatcagtac gagcaacgat tcggtatgcg ctccaccagc ctgctgaatg
    35281 tgattggctc caacaccatt gctatctaca ccacccatcg cattctggtt gaaatattca
    35341 gcttaactct gcttgcgcaa atgaacgcag cacgctggtc gccgcaagtc gaactgacac
    35401 tcctgctggt ttatcccttt gtcagtttgt tcatctgtac tgttgcgggc ttgctggtaa
    35461 gaaaactttc acagcgtgca ttcagcgatc tgttgttctc cccgccttca ctgcccgctg
    35521 ccgtcagtta ctctcgttaa accatcaccc cagccggggc gattcaattt gctaaaccat
    35581 gtcgattacg gataatgaac agcattgctt atcagagata tgcccccata tgttgaggca
    35641 tatcctaacg agaatctgac aaccgttatg cctgaacaat atcgttatac gctgcccgtc
    35701 aaagcgggtg agcagcgtct gctgggcgag ttaaccggcg cagcctgtgc aacgctggta
    35761 gcggaaattg ccgaacgtca cgccggtccg gtggtactca ttgcaccaga tatgcaaaat
    35821 gctctgcgtt tgcatgatga aatcagccag ttcaccgatc agatggtgat gaatctggcg
    35881 gactgggaaa ctcttcccta cgacagtttt tcgcctcatc aggacattat ctcctcgcgc
    35941 ctttccaccc tttaccagct accgacgatg cagcgtggca tactgattgt tccggtgaat
    36001 acgcttatgc agcgcgtttg cccacacagt tttctccacg gtcatgcgct ggtgatgaaa
    36061 aaaggtcagc gcctgtcacg agatgcatta cgaacccaac tggacagcgc cggttatcgc
    36121 catgttgacc aggtgatgga gcacggcgaa tacgccacgc gcggcgcgtt gctggatctc
    36181 ttcccgatgg ggagtgagct gccttatcgt cttgatttct ttgatgatga aatcgacagc
    36241 ctgcgggtgt ttgacgtcga cagccagcgc acgctggagg aagtagaagc gatcaatctg
    36301 ctgcccgcgc acgaatttcc gaccgataaa gcggcaattg aactgttccg cagccagtgg
    36361 cgcgatacct tcgaagtgaa gcgcgatccg gaacatattt accagcaagt gagtaaaggc
    36421 acattacctg ctgggatcga gtactggcag ccattgttct tcagcgaacc actgccaccg
    36481 ctgttcagtt atttccctgc caataccttg ctcgtgaata ctggcgatct ggaaaccagt
    36541 gccgaacgtt tccaggctga cacgctggcg cgttttgaga atcgcggcgt cgatccgatg
    36601 cgcccgctgt tgccaccaca atcgctctgg ctgcgggtgg acgagctctt ctcagagctg
    36661 aaaaactggc cccgagtgca gctaaaaact gaacatttac cgacaaaagc cgcgaatgcc
    36721 aatttaggtt tccagaaact gccagacctg gccgttcagg cgcaacaaaa agcgccgctg
    36781 gatgcgctgc gtaagttcct cgagactttc gacggtccgg tggtgttctc ggtagaaagt
    36841 gaaggtcgcc gtgaagcgct gggtgaactg ctcgcacgaa ttaaaattgc tccgcaacgc
    36901 attatgcgtc ttgatgaagc cagcgaccgt gggcgttatc tgatgattgg cgctgccgaa
    36961 catggttttg tcgataaggc gcgtaatctg gcgctgatct gcgaaagcga tctgctcggt
    37021 gaacgtgttg cccgccgtcg tcaggattct cgccgcacca tcaaccccga tacactgatc
    37081 cgtaaccttg cggagctgca tattggtcag ccggtggtcc atctggagca cggcgtcggt
    37141 cgctatgccg gaatgaccac gctggaagct ggcggcatta ctggcgagta cctgatgctc
    37201 acctatgcca acgacgccaa actgtatgtt ccggtgtcgt cactgcatct gattagccgt
    37261 tacgcaggtg gcgcggaaga aaacgccccg ctgcataaac ttggcggcga tgcgtggtca
    37321 cgcgcgcggc agaaagcggc ggaaaaagtg cgtgatgtgg cggcggaatt gctggatatc
    37381 tacgcgcaac gcgccgccaa agagggcttc gcgtttaaac acgatcgtga gcagtatcag
    37441 ttattctgcg acagcttccc gtttgaaacc acgccggatc aggcgcaggc cattaatgcg
    37501 gtacttagcg acatgtgtca gccgctggca atggatcgtc tggtgtgcgg cgatgttggc
    37561 tttggtaaaa cagaagtggc gatgcgcgcc gctttcctgg cagtagataa ccacaagcag
    37621 gtagcggtgt tggtgcctac cacccttctt gcgcagcagc attacgacaa cttccgcgac
    37681 cgtttcgcca actggccggt acgtatcgaa atgatctccc gtttccgcag cgccaaagag
    37741 cagacgcaaa tccttgcgga agtggcggaa gggaaaatcg atattctgat cggtacgcac
    37801 aaactgctgc aaagtgacgt caagtttaaa gatttaggcc tgctgattgt cgatgaagaa
    37861 caccgcttcg gggtgcgtca taaagagcgc attaaagcga tgcgcgcgaa cgtggatatt
    37921 ctgacgctta ctgcaacgcc gatcccacgt acgctgaata tggcaatgag cggaatgcgt
    37981 gacctgtcga ttatcgccac gccgcccgcc cgtcgtctgg cagttaaaac ctttgtccgt
    38041 gagtatgaca gcctggtggt ccgggaggcg atcctgcgtg aaattttgcg cggaggacag
    38101 gtttattatc tctacaatga tgtggaaaac attcagaaag ccgccgaacg gctggcagaa
    38161 ctggtgcctg aagcacggat tgccatcggt cacgggcaga tgcgcgagcg cgaactggaa
    38221 cgggtgatga atgatttcca tcatcaacgt ttcaacgtgc tggtttgtac caccattatc
    38281 gaaaccggga tcgacatccc gacagccaac accattatca ttgaacgtgc ggatcacttc
    38341 ggtctggcgc agctgcacca gttacgcggt cgcgtcggac gttcacatca tcaggcatat
    38401 gcatggctgc tgacgccgca tccaaaagcg atgactaccg atgcacaaaa acgtcttgaa
    38461 gcgattgcct cgctggaaga tctcggggca ggttttgcgc tggcaacgca cgatctggag
    38521 attcgcggcg cgggtgaact gcttggcgaa gaacaaagcg gctcaatgga aaccatcggt
    38581 ttctcgctgt atatggagtt gctggaaaac gccgtcgatg cactgaaagc cggacgcgag
    38641 ccgtcgctgg aagatctcac cagccagcaa acagaagtcg agctgcggat gccgtcgcta
    38701 ttgccagatg atttcatccc tgacgtgaac acgcgtctgt cgttctacaa acgtattgcc
    38761 agcgccaaaa cggaaaacga actggaagag atcaaagtcg agcttatcga tcgcttcggc
    38821 ctgctgccag atccggcgcg taccctgctg gatattgccc gtctgcgcca gcaagcgcag
    38881 aaactgggga tcaggaagct ggaaggtaat gagaaaggcg gggtgatcga atttgccgag
    38941 aagaatcacg ttaatccggc ctggttgatt ggtttgctgc aaaaacagcc gcagcattac
    39001 cgccttgatg gtccgacgcg cctgaaattt attcaggatt tgagtgagcg gaaaacgcgt
    39061 atcgaatggg tacgccagtt tatgcgtgaa ctggaagaga acgcgatcgc ttaatgtaaa
    39121 tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa ttctttgcac tttcctgcac
    39181 tatccgacac tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat
    39241 ttattatgta tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac
    39301 gttcgccgct gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc
    39361 agcgagcagt cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc
    39421 tctggaagcc atcgccaaaa aatataacgt cggctttctc gctctgttac aagctaaccc
    39481 cggcgttgat ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt tgcaaaccct
    39541 acttccagac gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc gtctctatta
    39601 ctacccgccg ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg
    39661 tgacacgctg acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa acccaacctg
    39721 gacgccaacg gcaaacatcc gcgcccgtta taaagcacag ggaattgagt tgcctgcggt
    39781 agtgccggct ggaccggata acccaatggg ccatcatgcg attcgtctgg cggcctatgg
    39841 cggcgtttat ttgcttcacg gtacgaacgc cgatttcggc attggcatgc gggtaagttc
    39901 tggctgtatt cgtctgcggg atgacgatat caaaacactc tttagccagg tcaccccagg
    39961 caccaaagtg aatatcatca acactccgat aaaagtctct gccgaaccaa acggtgcgcg
    40021 tctggttgaa gtacatcagc cgctgtcaga gaagattgat gacgatccgc agctgctgcc
    40081 aattacgctg aatagcgcaa tgcaatcatt taaagatgca gcacaaactg acgctgaagt
    40141 gatgcaacat gtgatggatg tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt
    40201 gagcccacaa actctgtaaa tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg
    40261 ggaaaaaatc agcagtagca ggcattaatg agggttaatg cttatttata aattactgct
    40321 gttccatgga gggtattcgg accggttaca gaagtaatac ggaaagattt tgcgcccatc
    40381 tcatccgctt tttgcgccag ctgctcttcc agcgatccca gatttgtccc cgcgttagca
    40441 ctgatagtac cgactttttg ttggccttct ggcgttgact gaacttcgac agccgcaaag
    40501 ttggcaaatg acatggagct taaaatcgcc gcagcgatga ggtttttacg tttttcataa
    40561 tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg ataactaaat
    40621 gatagatgtg atctggatca catacaagat attttttata acaatcatta attaatttaa
    40681 aaatctttaa atttcagtca aatagaaagc aattattttt atgggatctg gactggtgaa
    40741 cggaggtgat tatttttata ataacctttc attcacagtc accaggtaca acggcatcat
    40801 ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag
    40861 ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc
    40921 tcttgcggac ctcgtcgaag caaccggagc caaagcgccc acgctatacg cggaatttac
    40981 caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa
    41041 gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt
    41101 tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct tcatgatcaa
    41161 caactgcacc accctctccc cagattcagg agatatagcc aatacgttga aatcacgcca
    41221 tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat
    41281 cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat
    41341 gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg ccggaacgac
    41401 tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaggcccctc agcctgagcg
    41461 aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc
    41521 ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc
    41581 tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc
    41641 gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc
    41701 tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc tttaccgcga
    41761 ccaccaccaa actgatgccc tatcacgcca ccagcaacag cgccgagcac cgacccggta
    41821 atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga
    41881 cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg
    41941 ccccgttcaa acacgttcag actggccact gccgctacgc ccaacgcagc tgcgacacca
    42001 atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt gcagttatgc
    42061 caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg
    42121 actggaaata cttaaggatt cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc
    42181 gtcttatcag gcctaccgct cgtaccattt gtccagggcg gataaggcgt ttataccaca
    42241 tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc ggttaatact
    42301 ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct gatgcattcg
    42361 gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt
    42421 caggttaccc atcaggctac caacggtgga gaagttcgac agcgatacca gcgaaccatg
    42481 atctttatac tgataatttt tcagcggctt accgttcatc tgcgccagaa tgttgttcat
    42541 tgcgcaagtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg
    42601 acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcgag tggtttgcag
    42661 cgtcggttcc accaccagct ggttgatacg gttagtttca agaccaccga tatctttcag
    42721 gaagtctggc gctttgatcc cggctgccca taccatcaga tcagcctcaa tatattcgcc
    42781 atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagcacgcg
    42841 aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg
    42901 caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt
    42961 gtaaccgtag ctgtgcagtt gcttgatcgc gttgtgcaat tcagcggaga gttctacacc
    43021 cgtcgcgccg ccgccgacaa tcgcaatgtt cactttgcca tttgcgccca ggttggcaga
    43081 gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt gcgggttatc
    43141 gaggaaaatg cagttctctt tgacgcctgg cgtattgaaa tcgttagagg tgctacccag
    43201 cgccatgacc agggtgtcat aggcgatttt acgttccgga accaacagtt cacctttctc
    43261 gtcgcgtagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa tgacggaacc
    43321 caactggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc
    43381 ttcatcaagc gagccagtcg ccacttcgtg cagcagtggt ttccacaggt ggctgtggtt
    43441 acgatcgacc agcgtaattt tggctttttt cttacgtccc agcttatgcc ccagctgtgt
    43501 tgccatttcc agcccaccag caccgccgcc gacaatcaca atctttttca atggcgtagt
    43561 caacgtgccc cccttaaatt tattaaccaa ttgttaatta atggttatta acatagctct
    43621 taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata acatgaatgg
    43681 tgcattggtc ataccaaaat tgatgttaat caagttttgt tgcttaaaag ataggcaact
    43741 ttaccggggg agatcaaaaa atagagttta ttgtttatta tttgagcaaa ttcggaaggg
    43801 taatataggg tgaagcgtat atcgagggcg tttggtcgtc ctgataagat gcgcagcatc
    43861 gcatcaggca ttgatgctcg actgtcggat gcggcgtgga cgccttatcc gacttacaga
    43921 attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg
    43981 gttttgaacg ctttaatgcg ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc
    44041 tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg ctggctgtta
    44101 agtgcttcat cattacgcga caaaataacc agacaacgat cgcgattctt ctcgcggaag
    44161 ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc
    44221 atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa
    44281 ccaatccgtt ccgcccagta tccgcccagg ccaacgccgc aaattagcgg acgctcgtca
    44341 acgttcagtt gcaacatttt gtccacttct ttaagcagat gctgcatatc atgtttcgga
    44401 tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact
    44461 ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc
    44521 tcacataagc cagggttagt gacctacttt ctcttcctgc cagcgttcat gtaactgatt
    44581 cagacgggtg ctgatcgctt tccagcgagc cgagtccatc agttcctgtc gcgaaaatga
    44641 acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac
    44701 gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacccg catccagtga
    44761 tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc
    44821 agagaaaatc acgccgtcaa accccagttc ctgacgcaaa acggttttca gccagtaggg
    44881 agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc
    44941 gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc
    45001 cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg
    45061 tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg
    45121 gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc
    45181 gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat
    45241 ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcccaa
    45301 cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac
    45361 gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg cgcggatctg
    45421 gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc
    45481 tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt aaccttcgac
    45541 atccaacatt actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg
    45601 tgtcatcggc cagcctgata aattgttgat cgccggtttg tcgccagcgg tactcaaacc
    45661 accctccttt gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg
    45721 ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat
    45781 tttccaccca caccgccgcc agttccagcg cgatatcacc atctccagca tactcccagt
    45841 cgatgagttt taaccctgat gcgctatgca ctaaatttcc ggcgtggaca tccatatgca
    45901 atggacttaa gcgtaaaggt cgtggttccc gcgctttgcg cagacgtttt aacattcgca
    45961 gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaagggca
    46021 acagcgttat tcgccagcca aaacgtggtt gttgatgtag ataatacagc aagcctgcca
    46081 gttcgttggt atccggcaaa tacgttttta ccgcaccggg cagatagtcg actaccatcc
    46141 agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc
    46201 gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa
    46261 aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta ttaaacgtcg
    46321 tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat
    46381 tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc
    46441 tgcatttgta gggtcggagc gttaacgttg ccagaggcgc aggagtacag cacgtaatga
    46501 gcgccgacat tgcgggcaat gcctatggct ttactacggg tgcctaaact gtcctgcggc
    46561 gacaaaccta actgttgctt agccatcgac agttgctggg cggaaaccag ggtaaatttc
    46621 ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg
    46681 ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc
    46741 ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc
    46801 ggcgcaggcg gtgccgttcg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc
    46861 ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt
    46921 ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc
    46981 agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc ctttacaaat
    47041 atagataaag tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg
    47101 ccgaatgtgc gggaatggta acgctgcgtg gcctttccag aggatgcatc tccagccctc
    47161 tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa
    47221 tcgttgagga tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga
    47281 tgcccgcagc caataaagat gactccatca ccagcgattg ctcatcattc accggaattt
    47341 ccggatgtga acgacagccc accagcagta acaacaccag agacaccagc ccaaagcatc
    47401 cttttctcat cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa
    47461 caagtgcata tggatgtggt aaacctcttg tccgccatgg cggttggtat tcatgatcag
    47521 acgatagcca tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat
    47581 gcgtcccagc gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgagaatatt
    47641 cggaatgatc agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag
    47701 atcatcctgg tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt
    47761 ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc
    47821 gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaatc ttaactcacg
    47881 atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc
    47941 tgcgaatgcg ctgaaaagac attctctgtt tggtttacac gacaaatatg aatgcgtata
    48001 tttctcattt gcatttgcag caaaattatt cgcacactaa atataaccgc tcatcaccct
    48061 cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat
    48121 ttaacaggga taatcaatat caagtcatca ccaaaccgtc actacttgcc ggttgcatag
    48181 cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa acggtgattg
    48241 ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct
    48301 ctgcgggtac caaaatgcag atgactcaac atgatattcc tcagtcggtc actattgtta
    48361 gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc
    48421 tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc cgcggattcc
    48481 agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg
    48541 gcgacgcact ttctgatatg gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag
    48601 gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga
    48661 cctgtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt
    48721 atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg
    48781 gcggctacca gaataacgac tcatggctgg accgctacaa tagtgaaaag accttcttct
    48841 cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc
    48901 agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca
    48961 gcagcaacag ttacgatcgc gcacgcagta ccgcacctga ctgggcgtac aacgataaag
    49021 agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacac
    49081 tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg
    49141 taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatc
    49201 atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg
    49261 ctgacggtag ttatgaattg tttggtcgtc agcacaatct aatgtttggt ggcagttaca
    49321 gcaaacaaaa caatcgttca gttcatgggc caacatcttc ccggatgaaa ttggcagttt
    49381 ctacaacttt aatggcaatt tcccacaagc cgactggtca tcacagagcc tggcgcagga
    49441 cgataccaca catatgaaat cgttatatgc tgccactcgt gtcacccttg ccgatccgct
    49501 gcatctgatc ctcggcgcac gttataccaa ctggcgggtt gatacgctga cttacagcat
    49561 ggagaaaaac cacaccacgc cttacgctgg tctggtgttt gacatcaatg acaactggtc
    49621 gacctacgcc agctatacct ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa
    49681 atatctggct ccaatcaccg gtaacaacta cgagctgggt ctgaaatcgg actggatgaa
    49741 tagccgtctg accaccacgt tagccatctt ccgtattgag caggataatg tcgctcagtc
    49801 caccggtaca cctatccccg gcagcaacgg cgaaaccgcc tataaagcgg tggatgggac
    49861 agtcagtaaa ggggtggaat ttgaactcaa cggcgcaatt accgacaact ggcagctgac
    49921 atttggcgca acgcgctatc ttgcagagga taacgaagga aacgccgtta atcctaatct
    49981 gccacgcacc acggttaaaa tgttcaccag ctatcggttg cctgtcatgc cagagttgac
    50041 agtcggcggt ggtgttaact ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg
    50101 caccttccgc gccgagcaag gtagctacgc gctggtggat ctcttcaccc gctaccaggt
    50161 gacgaaaaac ttctcgttac aggggaacgt caataacctg ttcgacaaaa cctacgatac
    50221 caacatggaa ggttctatcg tctacggcgc accgcgtaat ttcagcatta ccggcacgta
    50281 tcaattctga ataacacctg taaaaaaggc agccatctgg ctgccttagt ctccccaacg
    50341 tcttacggat tagtggttac ggatgtactc atccatctcg gttttcaggt tatcggattt
    50401 agtaccgaaa atagcctgaa caccagaacc agcaaccact acgcccgctg cgcccagttt
    50461 cttcaggcca gcctgatcca ctttagacac atcagcaacg ctgacgcgca gacgggtaat
    50521 acatgcgtcg aggttagtaa tgttttcttt accaccaaat gcagcaacca gagccggtgc
    50581 catttcgctg gtacctgttg cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt
    50641 cagatccagt gctttaatca gcacgcggaa gatggtgtag taaacaatcg cataaccgat
    50701 accgacgatc ggtaacagcc acagtttgct gctgttacca gacagaacga tgaagtcgat
    50761 cagaccgtgc gagaacgacg taccgtcacg catccccaga agaatacaga ttgggaatgc
    50821 caggcctgcc agaatcgcgt ggatgatgta caggatcggc gcaacgaaca tgaagaagaa
    50881 ctcgatcggc tcggtgatac cggtcaggaa cgaggtcagc gccgcggaga tcataatacc
    50941 gcccactttc gcgcggtttt ctggtttagc agagtgccag atagcaattg cggcagctgg
    51001 cagaccgtac attttgaaca ggaagccacc agacagttta cccgcagtcg ggtcacccgc
    51061 catataacgc ggaatgtcgc cgtggaaaac ctgacctgct gcgttggtgt attcaccaat
    51121 ctgcatctgg aaaggtacgt tccagatgtg gtgcagacca aacggtacca ggcaacgttc
    51181 gatgaaaccg taaatgccaa acgcaactac cgggttctgg taagcagccc actgagagaa
    51241 ggtctggatt gcagaaccaa tcggcggcca aatgaaggac agcacaacgc cagtaaagat
    51301 ggcagccagg ccagaaatga tcggcacaaa gcgtttaccg gcaaagaagc caagatactc
    51361 aggcagctta atacggtaga aacggttaaa catgtacgct gcaatcgcac cggagataat
    51421 ccctccgagt acgccagtat ccgccaagtg tttagaggcg atttcttcag caggtaaatg
    51481 cagtaccagt ggcgcaacca cggccatggt tttaaccatg atgccatagg caacaactgc
    51541 ggccagcgcg gatacgccat cgttattggt aaagccgagg gcgacaccga tcgcaaaaat
    51601 cagtggcatg tttgcaaaga cggaaccgcc tgcttctgcc ataacatgcg atacaacggc
    51661 gggcagccag ctgaaattcg cggaaccgac gcccagcaga atacctgcga taggtagtac
    51721 ggataccggc agcatcagcg atttaccgac cttttgcagg ttagcaaatg cattcttaaa
    51781 cataattgag agtgctcctg agtatgggtg ctttttttac gttctcacgc gtggcaaggg
    51841 gggagagcct cgccgtgtac agggcatcta agcgcccttt atttattaca cagagtaaaa
    51901 taattcagtg ccaatttgtt tgacggctat cacgtttcaa cttcagatca ttggagaagt
    51961 tgcacatttt gacaaaacat atgtcacaat atttcaaacg cggttattca ccgcccaatt
    52021 ttcggcggtg aactttactc gttttagcca ttaattacga gctttaaaaa aactcattca
    52081 acggatggat tgaaggcggg aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt
    52141 tacctgcgcc agttcttcaa cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc
    52201 acgaaccatc gcaggttgat tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga
    52261 gtcagtttcc accagtaatc gatccagggg gacataacgc gcagcatcgc gcagttgctc
    52321 cgcattacgg aaggtcacaa tgccggaaaa ggagatgtaa aatccgagat ccagtaattt
    52381 acccgccgtt tctctgtcct ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt
    52441 ttcttcgcgc agaattgcca gcgtatcggc acgggcgtca cgggtatgga cgataaccgg
    52501 cttgttcagt tcacgaccaa tctggatatg atggatgaag gactcttgct gacgtacttt
    52561 agtttccggt gtgtaataat aatccagccc ggtttcaccc agcgctacaa caccctcttc
    52621 tgccgccaga cggcgtaaat cttctacatc gtagggatca ttctggttga gtggatgcac
    52681 gccacaagaa aatacgacgt tgtcacgttc gcctaccaga tcccgcatat gtaaataacc
    52741 cggtaatgtt gtggcgactg ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag
    52801 aacgtcatcc acgtccttat gcaaagattc ataatccaga ccatcgagat ggcagtgtga
    52861 gtcgactaaa aacatgatgt ctctcttaaa gatgaggaac cggtaacaca acgcccggtt
    52921 gcaggtaatg ctcaatacgc agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg
    52981 tgacagacat taactgttca cgaatgtggc aaacatcccc cagtatagcc tgcaggcacg
    53041 agggagaaag atggtttgcc agttcggcga ccaggcccgg cacatcaaca ttggtcacct
    53101 gcgcagcgcc atgatggcgt tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac
    53161 gcgccggagc ttgttcatga ttaagggccg ccagcagcga ataccagtcg cccgatggca
    53221 cgctatatgc caacgcctga cacaatgttt cacgagcctg ccagttatct ccctgaaaca
    53281 acgccagtgc cgcgccaggc gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct
    53341 gtgacattgt cacttcgcgt gaaagccaag tcacggcgta ctgttctgga ggcggcgcaa
    53401 ggtaatgtaa ccgacaacga ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag
    53461 ccaggaaaaa ccaggtttct gctggtggct cttcaagcgt tttcagcaat gcgttagccg
    53521 cggcgtcggt cagtaaggca gcatcggtta cccagacaac tttcgcacca cctaggcgtg
    53581 cgtgctcatt cagcttttcg gtgacctcac gtaccgcatc aatgcccagc gtatttttcc
    53641 ctttttcggg agccagggtg tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc
    53701 cacgacagtg accgcaactt ttgtggccct gcggttgttg gcagagtaaa taacggctca
    53761 gggcgtagat taaagcatca tcgcccatgc ccggtaacgc ctgaatgagt agcgcatggt
    53821 gacctcttcc ggcctgatag ctggctacca gtttttcgaa atcaggtcgt aaccatggat
    53881 accatctcat gcgtccaact ccttcaccca gtgggtcacg gtagtgcgga ttgcatccat
    53941 cacggcctcc agcggctggg tggcatcaat ggtatgaata cttttatctt gtgcagccag
    54001 ttccagatag cgggcgcggg tgcgattaaa gaaatcgaaa gactcttgct caatacgatc
    54061 cagctcgccg cgcgcacgcg cgcgttttaa gccaacttcc ggggtaacat cgagatagag
    54121 cgttaagtct gggcgaaaat ccccgagaac agcatcacgc agtgttgcca gcatatgttg
    54181 gtcaatacca cgtcctccgc cctgatacgc ctgagtggaa agatcgtggc gatcgccaat
    54241 cacccaggtg ccgttagcca gcgcaggttt gatgaccgtt tctaccagtt gaacgcgcgc
    54301 ggcataaaac atcagaactt cggctttatc ggtaatgact tcatcgccta ccgatttgat
    54361 atccagcacc aggcttctta acttttcggc aagttgcgta ccgccaggtt cccgagtgaa
    54421 aaccatgtcg cggataccca gttgctcgag cgtctcaacc accacattac gcgcggtagt
    54481 tttgcctgcg ccttccagcc cctcaatgac gatatactta ctgcgcattt ttttccttaa
    54541 gcactttcag ataatcctgc acagacttgt tatgactggc aagattggta ttaaacgtgt
    54601 gaccaccttt accatcggcc acaaaataga gatacggcgt ttttgccgga tgcgcagcag
    54661 ccttcagcga atccgccccc ggcgtagcta tcgcacccgg cggcaaaccg gtaatggtat
    54721 aggtgttata cgctgtcggc gtttccaggt ctgcacgaga aagtttgcca ttataacgct
    54781 ctcccatccc gtaaatcacg gttgggtcgg tctgcaggcg cataccaatg cgtaaacggt
    54841 tgataaatac cgaggcaacc tgatcgcgtt cactggcaac ggcggtttct ttttcgataa
    54901 ttgatgccat cgtcaccagc tgatttttat ctttataagg cagaccgtcc gcacgccctt
    54961 cccaggcgct atcgaccgct ttcaccattt tcttgtgaac tcgcttgagt aacgcgacat
    55021 cggtggtatt ggcggtatac atcaaggtgt ctggccagaa ccaaccttca atccactccg
    55081 ggttttccag ttcaagtgcc tgcgctacgg tggcgtactt atcgtcactc agcgtgtgct
    55141 tgatatacgg agcctcacgc aattgcttga ggtagtcgct cagacgcatc ccttctacca
    55201 gtcgcagcgg gaactgtgct tctttaccgc tttccaacaa tttcagcatc tcgcgcacgg
    55261 tcatctgcgg tgtaaagcgg taagtcccgg ctttaaagtg agaaagatcc ggttcgatac
    55321 gcagcagcca ttgaaaaacc cgtgggcgat tgatgatctt atcagcataa agctgttcac
    55381 cgagcgccag acgtccggtc cctggcttca gggtaaatat cgtctcttct ttgataagca
    55441 atttgctgtc ggcaagatgg cgaaccttcc agacgcccac accagcggcg atacccagta
    55501 ccaccagcaa taacaagata atcaataaca cttttttcat gactaattcg ggcgctcaca
    55561 aagtggggct aaatattcat ataacgttgc tgacgaaaag gtgacatcgc cacaggcacg
    55621 tacgggcatc actggcatta acgcattaca aataaccatc tcatctgcct gcaacgcctc
    55681 ctccagcgag gcttgcactt cgacaagctg ataagaggat tgtgccagta aacggataca
    55741 gaattgtcgc ataatgccgt taacacctgc ctgatccagt cgcggcgtat aaactacatt
    55801 gcccttccgc cagaacaaat tagccgcaca gcattccgta acccaccctt cgctgtcaag
    55861 gaccagcgcc tcatcagcgt ttgtctgctc aagatgagag cgaatcaata cttgctcaag
    55921 ccgattaaga tgtttaatac ctgcaagatg aggattgcgc cccagccgca ccgggcttag
    55981 cgccaacgtc atcccttcgt tacgcaaacg gtcgtaatgt gcaggataag ccgtaacgga
    56041 gagaatccgc gttgctggtc cgctgttcaa tgtgctgtac cctcgcccgc cactaccgcg
    56101 actgatcacg actttcagca caccattttg ctgttctgct gccagcgttt tcatctcttg
    56161 ttcaagctga ggccagaagt cacaggaaat catcaaccgc tgacaagcat cctgtagtcg
    56221 caggatatgc gccgataaca aactgacttt accgtcgata actctggcgg tggtaaaaca
    56281 accatcacca aactgcgttg cccgatcgct tactgccagc gattcctgct tataaccgtt
    56341 aattaagaac ataccggctc cttatcagag aattgttagt gtggcagcat gttcactacg
    56401 gaacaagtcg gaataaaagc taagaaaaaa ggcccgcaag cggacctttt atatgggtgg
    56461 aaaatgacaa cttagatctt tttaaagatc aaagaaccat tagtgccacc gaagccgaag
    56521 gagttacaca gcgtgtattc cattccgctg acctgacgtg cttcatgcgg tacgaaatcc
    56581 agatcgcaac cttcatccgg gttatccagg ttgatggttg gcggaacagc ctgatcgcgc
    56641 agcgccagga tggagtagat agattctact gcacccgccg cacctaacag gtgaccggtc
    56701 atagatttcg tggagcttac caacacacgg cttgcagctt caccgaagat ggttttcacc
    56761 gcctgcgctt cagctttatc gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca
    56821 atctgactcg cttcaatgcc tgcatcacgc agagcatttg ccattgccag agctgcgcct
    56881 gcgccatttt ctggcggtga cgtcatatga taagcatcgc tgctcatacc aaagccgacg
    56941 agttcagcgt aaattttcgc accgcgtttt ttcgcgtgtt cgtactcttc aagtaccagc
    57001 ataccggcac catcgcccag tacgaagcca tcacgctctt tatcccacgg gcggctcgcc
    57061 gcttgcgggt tatcattgcg ggtagataat gcacgtgccg cgccaaaacc accaacgccc
    57121 agcggcgtac tggctttctc tgcgccacct gcaaccatca cgtcagcatc gccatacgcg
    57181 ataatacgcg cagcatggcc aatgttgtgc acgccggaag tacaggcggt cgcgatagag
    57241 atgcttgggc cacgcaggcc atacatgata gtcagatgac ctgccaccat gttcacaatc
    57301 gttgacggaa cgaagaatgg gctgatctta cgtggaccac cgttcatcag agatgtgtgg
    57361 ttttcttcga tcagtccgag gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt
    57421 gcgttctctt ccgttatttc aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt
    57481 ccatattgaa tgaaggcatc catcttgcgc tgttctttgc gcgagataat gtcctcacag
    57541 ttaaaatcct ttactaagcc agcaaatttc gttgcatagg cgctagtatc gaaatggtcg
    57601 attaggctga tgccactctg accggcaagc agagctttcc aggtagactc tacggtattg
    57661 ccgacaggag acaacatgcc cagtccggtc acaactacac gacgcttaga cacgtttgtc
    57721 ctccaaggag ggaaaaatga ttctagtggg acaaaaagat aaaactcagg cggtcgaacg
    57781 accgcctgga gatgttcact tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa
    57841 cggtggtgat tttctcagct tcttcgtccg gaatctcagt atcaaactct tcttccagag
    57901 ccattaccag ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat
    57961 tgttggtaac ttcttcctgc ttaacgccca gctgttcgcc gataattttc ttaacgcgtt
    58021 cttcgatagt gctcatactc ttaaatttcc tatcaaaact cgctttcgcg atggttttcg
    58081 tagtgtataa aatgttgaaa aatttgcaac taaatcccgg caggtcttac cacgatttta
    58141 cgttattttg aggccttacc ccccaataac gcaaatcatt ttgcactaat tattactcat
    58201 aaccacgcag actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag accatgtaca
    58261 tcccgccgtt cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga
    58321 atgcaaccgc gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca
    58381 ggatacccgc acgctggtca tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg
    58441 gagcaacaac gtttacagta ataccgcgtg acgcaacttc gcgcgccagt gatttactga
    58501 agccgatcaa gcccgctttc gccgcagcgt agttggcctg accgccattt cccatggtac
    58561 caaccacaga accgatagtg ataatacgac catgacgctt tttcatcata gcgcgcatta
    58621 ccgcttttga cagacggaaa acagatgaaa ggttagtttc gataatatcg ttccactctt
    58681 catctttcat tcgcattaac aggttatcac gagtgatacc ggcattattg accaggatat
    58741 ccacttcacc aaattctgcg cgaatttttt ccagaacaga ttcgatagat gccgggtcgg
    58801 tcacattcaa cattagacct ttgccgttgg cacctaaata atcactgatc gcctgagcgc
    58861 cattttcact ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa
    58921 ttgcgcggcc aattccgcgg cttgcaccgg ttaccagtgc gatttttcct tcaaaattca
    58981 tgattttcct cttttaaagc tcgagcgccg ctgccatcgc tgaaggttcg ttcagcgccg
    59041 aggcggtcag ggtgtcgaca atgcgtttcg tcaggccagt aagcaccttg cccgggccga
    59101 cttcatagag atgttctacg ccttgcgctg ccatgtactc aacagacttc gtccactgaa
    59161 ccgggttata caactgacgt accagtgcgt cacggatggc atcaccattg gtttcgcatt
    59221 tcacatcaac gttattcaca acagaaactg ttggtgcgtt aaaggtgatt ttcgctaatt
    59281 ctactgccag tttgtcggct gctggtttca tcagcgcaca gtgagacggt acgctcactg
    59341 gtaacggcag cgcgcgtttt gcgccagcag ctttacaggc agcgccagca cgctcaaccg
    59401 cttctttatg accggcaata accacctgtc ccggagagtt aaagtttacc ggagaaacga
    59461 cctgaccttc tgcagcttct tcacacgctt tcgcaatagg cgcatcatcc agaccgatga
    59521 ttgccgccat agcgcccgtg ccttccggta cggcttcttg catgaactta ccgcgcatct
    59581 caaccagacg caccgcatca gcgaaatcaa tcacgccagc gcaaaccagc gcggagtatt
    59641 cccccaggct gtgaccggcc atcattgccg gtgctttacc gccctgctgc tgccatacgc
    59701 gatacagcgc aacagatgca gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt
    59761 cttcagctga cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt
    59821 cagcaaacgt ttcttcgaca attggatagc tcgccgccat atcagccagc attccaacgg
    59881 tttgagaacc ctgtccaggg aacacaaatg caaattgcgt catgttttaa tccttatcct
    59941 agaaacgaac cagcgcagag ccccaagtga atccaccgcc aaaggcttca agcagaacca
    60001 actgccccgg cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg
    60061 ccgcagaggt attaccgtgg cgatccagcg tcaccacgac attgtccatc gacataccga
    60121 gttttttcgc cgttgcactg ataatacgca ggttagcctg atgcggaacc agccagtcca
    60181 gttgagaacg gtcaagatta ttcgccgcca gcgtctcatc aacgatgtgc gccagttccg
    60241 ttaccgcaac cttgaagact tcgttgcccg ccatcgtcag atgaattgaa ttctctggat
    60301 tcacgcggtc ggcgtttggc agcgtcagca attcaccata actaccgtcg gcatgcagat
    60361 gggtggaaat gattcccggc tcttcagagg cagccagcac cgcagcgccc gcgccatcgc
    60421 caaaaataat aatagtccca cgatctgttg gatcgcaggt gcgcgccagt acatcggaac
    60481 cgacgaccag agcatacttc accgccccag atttcacgta ttgatcggct acgcttaatg
    60541 cataggtgaa acctgcacag gctgctgcaa cgtcaaatgc cgggcaacct ttaatgtcca
    60601 acatgctttg aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca
    60661 caacgatcag gccaatctgg tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg
    60721 cttcaaagcc catggttgaa acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac
    60781 cggtacgagt gacaatccac tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg
    60841 tccgcacttg ttcgggcaga tagctgccag taccaataat cttcgtatac atgtacgctc
    60901 agtcactttt cggttatata ccgtcacttg caaactgcga gttcgctggc agcgtcctgc
    60961 taccgcagag ttccgctttt gccaccgtcc agcagctcaa aaccagctgg gtatacagat
    61021 tccaggcgag cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca
    61081 atcgcgaccg caaaagctcg ctgattggct gcaccatgac tttttatcac cgtgccgcgc
    61141 aatcctaaca gacaggcgcc gttatactgg tcggggttga ggtgactgaa tcgcctcgtc
    61201 aggctctttt gtagccaacg ctttaataac agtagccacc acgaccgttt tttcccttca
    61261 ccctgagatt tcagcagaga aaggaacatc ctgacaacac cttccatcgt ctttaacgtg
    61321 acatttcctg taaagccgtc acaaaccagc acatctgtct tgccagttaa caactcattg
    61381 gcttcaagat agccgatata attgatagaa gggattgttt taagcaccgc tgaggcatcc
    61441 cgaatactgt caagaccctt tacttcttct tcaccaatat tgagcaacgc cacgcgagga
    61501 ttgggaattt ccaccacctc ttcagccaga actgagccca taatggcaaa ttgcaccaac
    61561 attgtgctgt cacaatcgac gttggcccct aaatcgagga ccaccgtttt gcccttttgc
    61621 tgatgtggta ataccgtcac cagcgccgga cgctcaatcc cctccagggg cttgagtaat
    61681 aattttgcca gccccatcag tgccccggta ttaccggcac tgacacaggc ttgcgctcga
    61741 ccttctttca ccagctccag ggccatacgc attgaactcc cacggctggc gcggatagct
    61801 tgcgaaggcc gggcatcact ggcgataact gactgcgcag gaataatctg cagacgcgaa
    61861 cgttgttcaa agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa
    61921 agaagagtga gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa
    61981 gggccaaaat cccctcccat gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg
    62041 ccgcccggtt tggtctttcc ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc
    62101 agacgcgtga ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt
    62161 ggtgacgcag gtgtttttca ccagaagttt tgtctacaga caggctggtg actgcggtca
    62221 gcgcgtcatg ggaacgacgc atgccacgtt tggaacgggt tggtttattc tgttgtacgg
    62281 ccatggacct tactcctcaa ttacggttta tccccgatcc aacggggagc accaattact
    62341 tacgctttaa gctggctaat acggcaaatg ggtttggctt ttgcacttct tcaggcagtt
    62401 caccaaagac catgtccgct tcggacactt cacagtgttc agaatcatgc accggaacta
    62461 ccggcaaggc gaggatgatt tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt
    62521 cgttaacctc aatcggttca tacgcttccg gcagtgcttc agcctgttcg tctgaacgca
    62581 caggactaaa acaatacgtt gtgtagacct gatgagtaaa cggcttcccg caacgctgac
    62641 actcgagcgt taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga
    62701 tagcgaacga catggagcat tccacatcac tgtccacact gactacggat tcggcgacgc
    62761 gctcaacctg atcaggggta tagataccct ggtaatcaag gcgtttttga gccgtacgaa
    62821 ccggatcgag agtcaggggt aattttacct tttgcatagg gcgcgcatat taactttgta
    62881 acgtcataga gtcaaagaaa aaggcagcct gtggttgcct tttgccaata attcgcacac
    62941 attgcgggtt actgctttat tttcgttcag tgctgataac gaacatcaac ctgcaaaacg
    63001 aagcgcatag tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc
    63061 taaacttatt ttagcctcca cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat
    63121 ctcttttgaa tgtgcagcac ctgaggtcga cgaaacccca cgcagcgacg aatcaccgcg
    63181 acagttggtg cttcgactgg cacaagaaaa atcgcaatct ctggcgtcac gttatccgga
    63241 tcatttaatt attggttcag accaggtatg tgttcttgat ggcgaaatca ccggtaaacc
    63301 gttaacggaa gaaaatgccc gtctgcaatt acgcaaagcc agcggcaata tcgtcacctt
    63361 ctatactgga ctggcgttgt ttaattcggc gaatgggcat ctacaaacag aagtggagcc
    63421 ttttgacgtc catttccgtc acctaagcga ggcggagatt gataattacg tgcgtaagga
    63481 gcatcccctg cactgcgcgg gtagctttaa gagtgaagga tttggcatta cgctgtttga
    63541 gcgtttagag ggacgtgacc ctaacacgct ggttggttta ccgctaatcg cgctgtgtca
    63601 gatgttacgt cgggaaggga aaaacccgct gatgggatag ttgtttgcgc cggatgcggt
    63661 tacgtcgcat ccggcaatcg gtgcaggcgg cctgatgcgg cgtaaacgcc ttatcaggcc
    63721 aaccgcccat tatcaggctt atatgcttgt attagcgcgc gttacgcagc ttttgcaaac
    63781 aacgcttcaa accttcatcc atcggcgctt cgatacgcat cacctcaccg gtccccggat
    63841 gggtaaactt caacgctgcg gcgtgcagga acagacgatt taatcccgtg cctgcttcag
    63901 tgagctgtct gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg
    63961 catactgtgt atgcacacgg atctgatgag tacgtccggt taccgggcta caacgcacca
    64021 gggtggcaaa tgcatagcgt tcttccactt taaagcgtgt ttccgacggt ttgccttcct
    64081 gactgacacg cacgatacgt tcgccgcttt gcagaatatt tttcaataac ggcgcttgaa
    64141 cgctcttcac atgcgactgc cactgaccgc gcaccagcgc caggtaatct ttttgcattc
    64201 ctttttcacg taattgctca tgcagagaac gcaacgccga gcgttttttc gctaccagca
    64261 acacacctga agtgtcccgg tcgagacgat gaaccagttc aaggaaccgc gcttccgggc
    64321 gcaacgcccg caaaccttcg ataacgccga agcttaaacc actgccgcca tgtaccgccg
    64381 taccggaagg tttattcagc accaggatat gatcatcttc atataagatg acgtccgcca
    64441 gcgccgccac cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa
    64501 ccggtgggat acgcacctca tcacccgctt cgagtttata ttcaggctta atacgttttt
    64561 tgttcacccg cacttcacct ttacgcaaaa tacggtaaat catacttttt ggtacgcctt
    64621 tcaattgggt acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa
    64681 caatttttac ggatggagtc tctgttttca tggtgggcga ttctaattag ccaacaggat
    64741 tcgcgccact catttttcta tgcttatttt tagttttgca ccttattact tcactacgtg
    64801 atcactttat tgatggttat taaaccaatc accagcaaga agtgaaaaaa ctgtgagtaa
    64861 gcgggtgata aatggtaaaa gtcatcttgc tataacaagg cttgcagtgg aataatgagg
    64921 ccgtttccgt gtccatcctt gttaaaacaa gaaattttac ggaataaccc attttgcccg
    64981 accgatcatc cacgcagcaa tggcgtaaga cgtattgatc tttcaggcag ttagcgggct
    65041 gcgggttgca gtccttaccg gtagatggaa atatttctgg agagtaatac ccagtctgtt
    65101 tctttgataa ttgcgctgtt tttccgcatg aaaaacgggc aaccgacact ctgcgcctct
    65161 ttgagctgac gataaccgtg aggttggcga cgcgactaga cacgaggcca tcggttcaca
    65221 cccggaaagg cgttactttg cccgcagctt agtcgtcaat gtaagaataa tgagtaagtt
    65281 acgatgaaaa gaatgttaat caacgcaact cagcaggaag agttgcgcgt tgcccttgta
    65341 gatgggcagc gtctgtatga cctggatatc gaaagtccag ggcacgagca gaaaaaggca
    65401 aacatctaca aaggtaaaat cacccgcatt gaaccgagtc tggaagctgc ttttgttgat
    65461 tacggcgctg aacgtcacgg tttcctccca ctaaaagaaa ttgcccgcga atatttccct
    65521 gctaactaca gtgctcatgg tcgtcccaac attaaagatg tgttgcgtga aggtcaggaa
    65581 gtcattgttc agatcgataa agaagagcgc ggcaacaaag gcgcggcatt aaccaccttt
    65641 atcagtctgg cgggtagcta tctggttctg atgccaaata acccgcgcgc aggtggcatt
    65701 tctcgccgta tcgaaggcga tgaccgtacc gaattaaaag aagcactggc aagccttgaa
    65761 ctgccggaag gcatggggct tatcgtgcgc accgctggcg taggcaaatc tgctgaggcg
    65821 ctgcaatggg atttaagctt ccgcctgaaa cactgggaag ccatcaaaaa agccgctgaa
    65881 agccgcccgg ctccgttcct gattcatcag gagagcaacg taatcgttcg cgccttccgc
    65941 gattatttac gtcaggatat cggcgaaatc cttatcgata acccgaaagt cctcgaactg
    66001 gcacgtcagc atatcgctgc attaggtcgc ccggatttca gcagcaaaat caaactgtac
    66061 accggcgaga tcccgctgtt cagccactac cagatcgagt cgcagatcga gtccgccttc
    66121 cagcgtgaag tccgtctgcc atccggtggt tccattgtta tcgacagcac cgaagcgtta
    66181 acagccatcg acatcaactc cgcacgcgcg acccgcggcg gcgatatcga agaaactgcg
    66241 tttaacacca acctcgaagc cgccgatgag attgctcgtc agctgcgcct gcgtgacctc
    66301 ggcggcctga ttgttatcga cttcatcgac atgacgccag tacgccacca gcgtgcggta
    66361 gaaaaccgtc tgcgtgaagc ggtgcgtcag gaccgtgcgc gtattcaaat cagccatatt
    66421 tctcgctttg gcctgctgga aatgtcccgc cagcgcctga gcccatcact gggtgaatcc
    66481 agccatcacg tctgcccgcg ctgctccggt accggtaccg tgcgtgacaa cgaatcgctg
    66541 tcgctctcta ttctgcgttt gatcgaagaa gaagcgctga aagagaacac ccaggaagtt
    66601 cacgccattg ttcctgtgcc aatcgcttct tatctgctga atgaaaaacg ttctgcggtg
    66661 aatgcgattg aaacgcgtca ggacggtgtt cgctgcgtga ttgtgccaaa cgatcagatg
    66721 gaaaccccgc actaccacgt gttgcgcgtg cgcaaagggg aagaaacgcc gaccttaagc
    66781 tacatgctgc cgaagctgca tgaagaagcg atggcgctgc cgtctgaaga agagttcgct
    66841 gaacgtaagc gtcctgaaca acctgcactg gcaacctttg ccatgccgga tgtaccgcca
    66901 gcgccaaccc cagctgaacc tgccgcgact gtcgtagccc cagcaccgaa agctgcaacg
    66961 gcaacaccag cagctcctgc acaacctggg ctgttaagcc gcttcttcgg cgcactgaaa
    67021 gcgctgttca gcggtggtga agaagccaaa ccgaccgagc aaccaacacc gaaagcagaa
    67081 gcgaaaccgg aacgtcaaca ggatcgtcgc aaacctcgtc agagcaaccg ccgtgaccgt
    67141 aatgagcgcc gcgacacccg tagtgaacgt actgaaggca gcgataatcg cgaagaaaac
    67201 cgtcgtaatc gtcgccaggc acagcagcaa actgccgaga cgcgtgagag ccgtcagcag
    67261 gctgaggtaa cggaaaaagc acgtaccacc gacgagcagc aagtgccgcg tcgtgaacgt
    67321 agccgccgcc gtaacgatga taaacgtcag gcgcaacaag aagcgaaggc gctgaatgtt
    67381 gaagagcaat ctgttcagga aaccgaacag gaagaacgtg tacgtccggt tcagccgcgt
    67441 cgtaaacagc gtcagctcaa tcagaaagtg cgttacgagc aaagcgtagc cgaagaagcg
    67501 gtagtcgcac cggtggttga agaaactgcc gctgccgaac caattgttca ggaagctcca
    67561 gctccacgca cagaactggt gaaagtcccg ctgccagtcg tagcgcaaac tgcaccagaa
    67621 cagcaagaag agaacaatgc tgataaccgt gacaacggtg gcatgccgcg tcgttctcgc
    67681 cgctcgcctc gtcacctgcg cgtgagtggt cagcgtcgtc gtcgctatcg tgacgagcgt
    67741 tatccaaccc agtcgccaat gccgttgacc gtagcgtgcg cgtctccgga actggcctct
    67801 ggcaaagtct ggatccgcta tccaattgta cgtccgcaag atgtacaagt tgaagagcag
    67861 cgcgaacagg aagaagtaca agtgcagccg atggtgactg aggtccctgt cgccgccgct
    67921 gtcgaacctg ttgttagcgc gccagttgtt gaagaaatgg ctgaagtcgt agaagccccc
    67981 gtaccggttg ccgaaccgca accggaagtg gttgaaacga cgcatcctga agtgattgcc
    68041 gccgcggtaa ctgaacagcc gcaggtgatt accgagtctg atgttgccgt agcccaggaa
    68101 gtcgcagaac acgcagaacc gatggttgaa ccgcaggaag agacggcaga tgcagatatt
    68161 gaagaagttg ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct
    68221 gccgcgccag tcgtcgctga agtcgcagca gaagttgaaa cggtggccgc ggttgaacca
    68281 gagatcaccg ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa
    68341 tatgttccgg aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt
    68401 aaaggtgccg caggtggtca tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg
    68461 caacctgttg agtaataatt agctcaaagt aatcaagccc tggtaactgc cagggctttt
    68521 ttatttcatg tttgaatcaa catattaaaa ttcatcaaat ggcatccttg ctaaccaaca
    68581 atgcaaaata ggcaaaaaaa atgcccgccc cggcgatccc agagcgggca gacaaaacat
    68641 acccagtttc atgatatgtt ttaaagcgag cttatttgtt gagctggaag agcgacaatc
    68701 cctgcatatc ggtaaatgct ttatacgatg cctgcaatgc cgtttgctgc atgatgtaag
    68761 atgaaatagt tgcattccag tccacatcaa ccagatcgct catctgctgc gtttgcccta
    68821 aagcgcgatc gctacctaag gaatccagcg actccagttt gttcagctgc gtgcctaatt
    68881 ccgcgcgaac ggtaaggaca ttattcagcg agtttttcag tccacggttg gttttatcca
    68941 atgccgcagc ggcgatttct ttatccgctt cgctatccgc gaccggcgtt ttcagggctg
    69001 cgatggcgct atccagcatg gcaaaaagat tggtttcaga agcgctaccg tctggttccg
    69061 ctaccgcgtt actggtaata ctgtcgaaaa ttttgtcgcc cgtatgtcct atcaccatcg
    69121 aacgcgaagc atcgacctgt tgcttaatac tttctgttcc gccaacataa tcaccgttag
    69181 cctcactaaa cggcgcagtc tctgttttat atgtagcgcc cattaccgtc agtggtgttc
    69241 gccagattca gcaactggtc acgaagcccc tgaatatccg tagccagcga ggcccggtca
    69301 tcgtcactca aggtgccatt gctggcgtag acaatttttt cctgagcatt ctggatagca
    69361 gtggtgacct ggctaagtac actctcttcc agtgacactt tttgagtggc gaaagtacgt
    69421 gccagcgtgt actggctgtt ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca
    69481 gcaatgggat cgtctgaagg gttaacgact cgcttacccg tcgacatctg ctcgccgtac
    69541 ttcatccatt ctgcctgaga attggtgatg ccgcgcatgt tttgctggta catcatctgg
    69601 gtactgaaac gcattttatc tccccttagc gaatgttaat cagcgcatca aaaatcgcgt
    69661 ttgccgtctg cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc
    69721 cgtactcctc atcgagattg acaccggaaa tcgactgctg ctgattggaa agctgcgtca
    69781 ccacattacc ttgcgtggtg ctactggttt tcaacgtcgc ggttttatta ccgatatcac
    69841 tcactaacga ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc
    69901 tttgcagatc cagcagggcc tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc
    69961 tcgccatcgc gattttcgct tcgtcggtga ttaatacatc catgttgacg atggcgtcac
    70021 ttactggttt cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca
    70081 gaccatcaaa tgccacttta ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg
    70141 caaggcgggt gacctgccac tgattattat cgaacgagat tttgtaatct gtcgccagta
    70201 ccacggaggc atcagttacc gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt
    70261 gcagaaccgc gggcttaccg atagtaaaga aatcttcacc agcatcgccg ttagcatcaa
    70321 atccggcttt gtgttggctg ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg
    70381 tattacgcgt ctggtccaga tcctgagaac ggaatgtcag aatgccgccc agcgacccgg
    70441 tattcagtaa tttctccggg atctcaatat tgcctgccgt cccatcaacg taagcgacag
    70501 tcgtacgaga agggtcggcg ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct
    70561 gaaccagtga gtaaccattg gcatcgtgat gttataagta ccgccatcct gaacgctgac
    70621 ttctacgcca acaatctggt ttaattcgct caccagttga tcgcgttgat ccagcagatt
    70681 gttaggtgac gcccctgccc ccacgcctgt caggcgcgag atttgatcgt tcaggctggc
    70741 aatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg
    70801 tttgtcctgg tcgcgcagat attgatcggt ggttttaaac tgattcacca acccttctga
    70861 tttcccaatc agcgcctggc gcgccgccgg gtcttccgcg ttactcacca gcgtttgcag
    70921 gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt
    70981 gtcgattttc gacatctgct catagcgggc ggtcaggccg ctactttgcg tctgcgcagc
    71041 acgtaactgg ttggtaataa acgtatcata ctcacgctgc acaccagaaa cgtagacgcc
    71101 attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa tagtggtttg
    71161 gcgggtatat ccggcaacgt tatagctgga gatattatta cttgccgtat ttaacgccgc
    71221 ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta
    71281 taagccttca attacagagt attatcggca gcgacccgcc ggacttgagt tattcagaac
    71341 agattatcaa tgttcatgct gtaggttttg ctcaccttgt cgcttatcga tttcatctgc
    71401 tggatcatgt tggtgagttt acgggcatag tgaggatcgg tagcatagcc cgcgtcctgt
    71461 aggacctgcg ccccctgttc cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta
    71521 cgtgttaaca gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaac
    71581 ttcgctttta ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat ttcagtgact
    71641 tgccctttcc agttgccaga ggctttgaca ccaaacaggt tatagcttgg ctcgccgttt
    71701 tcgcggcgga tttgccgttg cccccaacca gattcaagcg cagcctgagc gaggatcaaa
    71761 tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg
    71821 aatgctctac tgtcacccgg cagcgaatca tcgtagttac gtggtacggc cttttgcacc
    71881 agctgcgaaa gcgtctggtt ttgataacgc accacggttt cgagcgggaa tttcatcggt
    71941 gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgttt aaccatcatc
    72001 tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatttgctg gtcatacata
    72061 ctggtataca gacgagtgtg ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc
    72121 atgcttttca acatcatctg cacgaacatc ccttccacct gacgggccac cggacggata
    72181 tttgccgccg gatcttcgct cgctttcgcc tttagttcgt tgagtgattg cgcatcccag
    72241 gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca
    72301 gacatcccgc actttgcatt gattgcaaaa tagacatcag atccatcggc gtagcgccca
    72361 gcgcattgag cgcacgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg
    72421 aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aacaacagtc tgtccaccac
    72481 caaacggtgt atctggctgg ctgacattgg cctgacgatt aactgttact gagagatttc
    72541 cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt catcaccacc gaaccggtgc
    72601 gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat
    72661 cggcaaggaa acggacctgg gaactgttgc cacttggtac gcgcacctga atagtccgcg
    72721 catctaacgc ggtggcgctg ccatatccac gcacgcggtt gatggtgtca gcgatttgct
    72781 gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact
    72841 ggctgggcaa ttcacgttca ataaccgcac cattggtgat ccgtccaccg ttcagttggt
    72901 taacctgaac actgctaccg cctgcggagg ctcctgcgcc gccaaccaga atattgccct
    72961 gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg agtcatcaac aacgtacctc
    73021 cacgcaagct tttggcatta cccatggaag aaaccaccac atcgatggtt tgcccctgac
    73081 gtccaaacgg aggaagtgac gctgtcacca ttaccgcagc gacgtttttt agctgcatat
    73141 tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg
    73201 taaacggcgt ctgggttgtc tggtcaccag tgccatccag ccccactacc aagccatagc
    73261 caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct
    73321 gagccgccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc accacagcct
    73381 cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc
    73441 gttaatgtag ccattgccta cgtattcaat acgcgcatcc gccacctgag tagacggtac
    73501 ggtattgctg ccgctgatag tgcgtggatt aaccacgccc gagaagcgaa taaattcggt
    73561 accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt tgaccagtac
    73621 ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg ctggcattgg ccccgccctt
    73681 tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacagccc
    73741 ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt
    73801 cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc
    73861 aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc
    73921 agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt
    73981 cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa
    74041 caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa
    74101 tcagtaaacc ggtgaacctg ccaccggtta tgccttagag ttgcgtcagt ttttgcagca
    74161 tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca
    74221 tattgaccag ttcttccgcc acgttgacgt tagacgtttc aacataccct tgatacagca
    74281 gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg
    74341 tgtagaggtt ttcgccaatg ctctccagtc cggtatcatt cataaaggtg gtgagattga
    74401 gctgcccaac ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac
    74461 gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac
    74521 caccagccgt caccagctgc ccgttctgat ccacctggaa agagccgtca cggatatagg
    74581 ctgatgagcc atctggcaac attacctgga aaaagccctg ccctttaatc gcgacatctt
    74641 tgctgttatt ggtctgcgac agattgccct ggctgtgtaa gcgttcagtg gcgaccgggc
    74701 gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc
    74761 ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac
    74821 cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt
    74881 caaggcccgt tttggcgatc cataatgaac tgatcatata atgtcctgta gtggattgct
    74941 gacaagcaga gcagaagcac gcgacagtgt gaatttcccc tcaccctaac cctctcccca
    75001 aaggggcgag ggaatagtcc gcgcgctttt attgcaatgt catcaatcgt tcaattaact
    75061 catcgacagc agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat
    75121 ctgcatttca aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac
    75181 gttactgcct tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg
    75241 ccctcgcgtg gcctgggttt ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac
    75301 ttcgctgccc gtggctttca ccagtttcag acgccctact ggcgcaactg tatttgccgg
    75361 atcgcccgga ttgagcgccg agattgtgcc atcggcagca atcgtgattt ccgccccttc
    75421 cggcacagca attggccccg cctcgcctat caccggatgc ccctgaattg tcagttgccc
    75481 ggtgggatca acctgaatgc tgccattacg cgtataccct tcgctgccgt cagcggtctg
    75541 cacggccagc catcctgctg caacgcaacg tccagcgggc gagaggtgta atccatcttg
    75601 ccgggcgtca tgtctgcacc cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa
    75661 agcccttcca ccggcactgc gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag
    75721 gcattggcca gattactggc ggttaccgcc tgttgattca gcgtctggct ggctgctccc
    75781 atcgcagtat aaattgcgtg atccattgag ctatcccgtc agcgattagc gtaagttaac
    75841 ccgcgtgttg aggatctggt cctgggtttt gatggtctgg gcgttagact tatagttacg
    75901 ctgggcaacg atcatattga ccagttcttt actgagatcg acgttggacg cttccagcgc
    75961 accgttggtc agagtgccaa agtttcccgt cccggctgtc cccaacagcg ccacgccaga
    76021 agattgcgtc gcagaccaga cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa
    76081 gttcgccagt acaatctgcc ccagcagttg ggtttgttcg ttggaattgt tgccgacaac
    76141 cgtaccgtca tcattgattt gataactcac cagatcgccc ggtttgtagc cattctgggt
    76201 ggttgccaca atgttgttag cgccggttgc gatattattc gccatcgcac catccactaa
    76261 tgtgccatta gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact
    76321 gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaaat agacgctcat
    76381 gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc
    76441 cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat
    76501 ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt
    76561 ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttaccgggt aacccgtcag
    76621 ctgtaaacct tgcgtattca acaggttacg gttttcatcc agcttaaatt gtccgttacg
    76681 gctgtagaac accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc
    76741 aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc
    76801 ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt
    76861 gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt
    76921 ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa
    76981 tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag
    77041 atccagcgta ttaccgctgt tgccgcggat cacaccctgc accagagcaa actgcagcgg
    77101 ctgggcaacc agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt
    77161 cggcgcagtg ctgccatcag tcaacgaacc gtcccaggtg aaactgtgaa ctccggcggt
    77221 cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt
    77281 aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc
    77341 ttcttcactg ccggttcccg caagaacagt tgtgccgggg atcatcacgc cgtgaccgat
    77401 caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt
    77461 ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc
    77521 gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca gcaaagtcag
    77581 aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact
    77641 gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat
    77701 tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg
    77761 gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc
    77821 ggcattttta tgtagccctt tgcatctgcc agcggattac ctggttcata aaccagtttg
    77881 tccggggcct gactttctta acatcggcaa cctttacgcc gcctgtcgca gcacctggtg
    77941 cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca
    78001 cgctatcagc atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta
    78061 acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta
    78121 aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca
    78181 ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacggtatta ccgtcaagcg
    78241 aaggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc gcctgcgccg
    78301 ggatgtgctg cgttgaggtc atcgtcagtg caaccacact ggttgcatcc cgtccacgtt
    78361 gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat
    78421 cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct
    78481 cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg caggtatcaa
    78541 aattctgcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata aacgcaaaat
    78601 gggtcgctat ttatgccgtt gatggtcatt gcggacaggt acaattcacg ttgtagaaat
    78661 ggctgggggc gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg atactgttca
    78721 gtccgttaag tacggcgagc aatctcacat cgcaattgca caactttttt agcgcccaac
    78781 tcgcgggggt aagtgatgag gttcgtgttt ctattcgtac agcgcccaat ctactaccgc
    78841 catgcgagca gccattgctt tcgatgagca ataattcccg cctgtggggc aatgtgaatg
    78901 tgttggcacg ctgcggtaac gacaaacgat atttacaggt taatgtgcag gccacaggaa
    78961 attatgtggt tgccgcgatg cctattgtgc ggggaggaaa gctggaagct ggcaatgtca
    79021 aactgaaacg tggacgcctg gataccctgc caccgcgtac ggtgctggat atcaatcaac
    79081 ttgttgatgc cgttagcctg cgcgatctat cacccgatca acctatccag ttaacccagt
    79141 ttcgccaggc atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc agcggtgatg
    79201 ggtttagcgc caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca cagaatgcgc
    79261 gggtgcgcat ggtatcggaa caggtagtca gcggcgttgt tgatgcagat gggaatattc
    79321 ttataaacct gtaagctgtt aaagattacc cgtcccttgc cgataaataa gcaacacatg
    79381 ataaaagcgc cctcaatgag gaataaacca tgagtattga tcgcacttcg cctctgaagc
    79441 ctgtaagcac cgttcaaccg cgcgaaacca ccgacgcgcc ggtaacgaac agccgggcgg
    79501 caaaaacaac cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga
    79561 tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc
    79621 gtaacggtga actaaaaatg gacaccggca aaattgccga tgcgctgatc aacgaagcgc
    79681 agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct cgaccagatg
    79741 tccgctgtgc ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg
    79801 gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg
    79861 acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat
    79921 attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa
    79981 cataacggct ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg
    80041 aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac accccatcgc
    80101 ggcagtaaaa agatttcgat ctgaatatta tctccggcct gcactgcagg ccggaatgca
    80161 ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc
    80221 gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat
    80281 ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac
    80341 cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat
    80401 tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat
    80461 agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt
    80521 taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa
    80581 gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa
    80641 cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac
    80701 ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagtgcca acaggaaaca
    80761 aagacgcaac ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact
    80821 tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac cggagggaaa
    80881 ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc
    80941 aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc
    81001 catctgtttc accacgcgca ttgctccagc atcgtggaag ttaatgcgcg gcaggaccag
    81061 catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcagaatgc cgccgaccgt
    81121 aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa
    81181 accaatcatg ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca
    81241 cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt
    81301 aatctttagt agctggctgg tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc
    81361 ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc
    81421 aagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt
    81481 acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat
    81541 acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc
    81601 gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt
    81661 catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa tccggaccca
    81721 cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa tagtctacct agattatggt
    81781 gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt
    81841 caacgatacg ttgcgccagc actgcctatt cgccggcggt ttgcggaact gtctgatttt
    81901 acacgcattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgctca agagtgctct
    81961 gccaaccagg aatcggttta ttcactacac cctgcccgcg ctcctcacgc cattcgcgca
    82021 tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc
    82081 cggcacggcg atgcatacaa gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg
    82141 caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc
    82201 cgcccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat
    82261 aaagatcgtg tggcccgacg ctattgctac gatgtttatc cattcttagc gaggctgcgg
    82321 tggcgagttg cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca
    82381 tcaacgtcag ttttttccgc gccgccagtt ccaccagcct ttcagcatcg cgcagatttt
    82441 ctgccagcgg tttatcgaca cagacatgta cccccgcatt gagtaacgta ctgaccacgt
    82501 caaagtggct ggcggtgctg gagtgcacaa aaaccgcatc acaactggcg gcaaggctgg
    82561 ataacgaatc ggcataagga atgcgccagc tgtcacaaat tggcagggct ttggcgcgcg
    82621 taggcgacca ggctccttgt aacgtccagt cagacgctgc cgccagcacc ggtaaccacg
    82681 ctttttgcgc aatgccgcct aatcccacta cgccgatacg taattttttc actttaatct
    82741 cccagatggg ccagcaacga atcaagacgt tgtttcagtt ctgccacttc gatttccagg
    82801 gcttcgacgc gggtctgtaa atcaccgtca accatgttcg acatagccgt taccgccggc
    82861 tgatcttcaa cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg
    82921 cgggccagac gcaccacaaa aggaccatct tcgcgattcg ccagttgttc cagcgtcgac
    82981 tccacttccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcaccc
    83041 ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttctgc ggcgctcagt
    83101 ttcagatcgc caaattctga attacaaaaa cgttgctcat atttggtgac ccgattacca
    83161 aaaccgctca ctgtgcgtag ataatgacgt ttgaccagat tatccagctg ttcctgcact
    83221 tcggattcac tcagattcat gaccggttca cggttcgttt tctgattaca ggccgtgact
    83281 acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa
    83341 ccaattacgc gagcttccag ggcagtaagt tgatatttca tgagattctc cttagcggcc
    83401 gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat
    83461 caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc
    83521 gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg
    83581 catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg
    83641 cccaatcgaa taaccgagat agcaggcatg aaaagagccg cgaacaacat tggaaaaatt
    83701 ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc
    83761 ttgtttatga aattcgttaa tcatccctag cctggcctgc cagcctgatg gataacagtg
    83821 gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc
    83881 cgccagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa
    83941 gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac
    84001 gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa
    84061 aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt
    84121 gataaaaaaa tactgtcccc gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa
    84181 agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct
    84241 gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt
    84301 cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat
    84361 tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg gcgcaattgc
    84421 cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt
    84481 cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg
    84541 actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc
    84601 acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgcca gtgcggtcat
    84661 tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg
    84721 ggccgttttg tttgtgctat gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact
    84781 ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt
    84841 tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgat
    84901 gctgccaatt atggtcaacg acgtggctgg cgcgccctct gccgttaaat ggatgtatgc
    84961 cattgaagcg tgtctgtcgt taacgttgct ctaccctatc gcccgctgga gtgaaaagca
    85021 ttttcgtctg gaacaccggt tgatggctgg gctgttgata atgtcattaa gcatgatgcc
    85081 ggtgggcatg gtcagcggcc tacaacaact tttcaccctg atttgtctgt tttatatcgg
    85141 gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc
    85201 tcgcggcagc tatatggggt ttagccgtct gggtctggcg attggcggcg ctattggtta
    85261 tatcggtggt ggctggctgt ttgacctggg caaatcggcg caccagccag agcttccgtg
    85321 gatgatgctg ggaattattg gcatcttcac tttccttgcg ctgggttggc agtttagtca
    85381 gaaacgcgcc gcgcgtcgtt tgctggaacg cgacgcctga tttgatactc ccccctgact
    85441 cctccatact gaaagtgaac tgacagccaa atggaggagt cacgtgaagc tatacattta
    85501 cgatcactgc ccttactgcc tcaaagcccg catgattttc ggcctgaaaa atatccccgt
    85561 cgaattacat gttctgctca acgacgacgc agaaacaccc acccggatgg tcggtcaaaa
    85621 acaggttccc attctgcaaa aagatgacag ccgctatatg ccagaaagca tggatatcgt
    85681 tcactatgtc gataaactcg acggcaaacc gttactgacc ggcaaacgtt cccctgccat
    85741 tgaagagtgg ctgcgcaagg tcaatggcta cgtcaacaaa ctgctgttgc cgcgttttgc
    85801 caaatcggca tttgatgagt tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa
    85861 agaggccagc gcgggtaatt ttgccgacct gctggcccac tctgacggtc tgattaagaa
    85921 tatcagcgat gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg
    85981 cgaactttcg gaagatgata ttcagctatt tccgctactg cgtaatctga cgctggtagc
    86041 cggaatcaac tggccaagcc gcgttgctaa ttaccgcgat aatatggcga aacagacaca
    86101 aatcaatttg ttatcatcaa tggcgattta atcctgaccc gccgcggcag cctgtattcg
    86161 cgggttgccg ccgttttcag ttttttctgc tggcgagttt atcgctggtg acacatgctg
    86221 cgttgtgtca ggatattgac gcagatttca tgtcgaggac taccatgaac aagtttttat
    86281 ttgccgctgc attgatcgtc agtggcctgc tcgttggctg taatcaactc acccaataca
    86341 ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca taataatttc tcaaaagata
    86401 tcggtttacc cggcgtggct gacgcccata ttgttctgac aaacttgacc agccaaattg
    86461 gtcgcgaaga gccgaataag gttaccctaa ccggagacgc caatctggac atgaactccc
    86521 tgttcggtag tcagaaagcg accatgaaac tgaagctgaa agcgctgccc gtgtttgata
    86581 aagaaaaagg tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg
    86641 aaaaaatgca aacggtgatg caaacgttgc ttccctattt gaaccaggca ttacgcaatt
    86701 actttaacca gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc gaagcaatgg
    86761 cgaaaaaact ggcgaaaggc attgaagtga agccaggcga aattgtcatt ccgtttacgg
    86821 attaatgacg agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct
    86881 tatcctttgt gtccggcaaa aacatccctt cagccggagc atagagatta atgactgcac
    86941 catcccaggt attaaagatc cgccgcccag acgactggca ccttcacctc cgcgatggcg
    87001 acatgttaaa aactgtcgtg ccgtatacca gcgaaattta tggacgggct atcgtaatgc
    87061 ccaatctggc tccgcccgtg accacagttg aggctgccgt ggcgtatcgc caacgtattc
    87121 ttgacgccgt acctgccggg cacgatttca ccccattgat gacctgttat ttaacagatt
    87181 cgctggatcc taatgagctg gagcgcggat ttaatgaagc cgtgttcacc gctgcaaaac
    87241 tttacccggc aaacgcaacc actaactcca gccacggcgt gacgtcaatt gacgcaatca
    87301 tgccggtact tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat ggtgaagtga
    87361 cacatgcaga tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc gtgatggaac
    87421 ctctgcgcca gcgcctgact gcgctgaaag tcgtttttga gcacatcacc accaaagatg
    87481 ctgccgacta tgtccgtgac ggaaatgaac ggctggctgc caccatcact ccgcagcatc
    87541 tgatgtttaa ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg tattgtttac
    87601 ccatcctcaa acgcaatatt caccaacagg cattgcgtga actggtcgcc agcggtttta
    87661 atcgagtatt cctcggtacg gattctgcgc cacatgcacg tcatcgcaaa gagagcagtt
    87721 gcggctgcgc gggctgcttc aacgccccaa ccgcgctggg cagttacgct accgtctttg
    87781 aagagatgaa tgctctgcag cactttgaag cattctgttc tgtaaacggc ccgcagttct
    87841 atggcttgcc ggtcaacgac acattcatcg aactggtacg tgaagagcaa caggttgctg
    87901 aaagcatcgc actgactgat gacaccctgg tgccattcct cgccggggaa acggtacgct
    87961 ggtccgttaa acaataaaaa tcgtagcttc ctgttgtcat taggttattc tacctgtata
    88021 aataaccagt atattcaaca gggggctatt atgcgaattg aagtcaccat agcgaaaact
    88081 tctccattgc cagctggggc tattgacgcc ctggctggcg aactttcccg ccgtattcag
    88141 tatgcgtttc ctgataatga aggccacgta tcggtacgtt atgccgcagc gaataattta
    88201 tcggttattg gcgcaacaaa agaagataaa cagcgcatta gcgaaattct ccaggaaacg
    88261 tgggaaagcg ccgatgactg gtttgtcagc gaataatatg cagtgatttt tgtttttgcc
    88321 ggatcgcccc ggctttttta ttttacttac gaattttgag tacaaaattt ctacacttcc
    88381 tcaaaataat tgcacaatat gctaaaaaaa tatgaatgac atgctgtttt attgttcata
    88441 taaatcttaa aagcagaaat ttatctgttc ccccctttaa aaattcggtg aataccctta
    88501 cttattggta tactgaagtt gcttcagata aaaacgcatt gaacctcgaa taacgttgtc
    88561 tagtaacacg aattaggggg ccatgatgga aaaaaataat gaagtcattc agactcatcc
    88621 gctcgtaggg tgggacatca gcaccgttga tagctatgat gcgctgatgt tgcgtttgca
    88681 ctaccagacc ccaaataagt ccgagcagga agggactgaa gttggtcaga cgctctggtt
    88741 aaccactgat gttgccagac aatttatttc gatattagaa gcaggaatcg ccaaaattga
    88801 atccggtgat ttcccggtaa acgagtatcg gcgtcattaa catgacatga ccatccaccc
    88861 aaaaggcacc tcaccggtgc ctttaccatt tctgggttgt atatctgcat tcagttaatt
    88921 actctgctta aaccatgaga gcagagagaa aaaaatgaaa tacgatctca tcattattgg
    88981 cagcggttcc gtaggcgctg ccgccgggta ttatgcaacc cgcgccggtt taaacgtgct
    89041 aatgaccgac gcccatatgc caccgcatca acacggcagc caccacggcg atacgcgatt
    89101 aattcgccat gcttatggtg aaggcgaaaa gtatgtcccg ttggtcctcc gcgcgcaaac
    89161 gctgtgggat gaactttccc gccacaacga agatgatccc atttttgtac gctctggtgt
    89221 cattaacctc ggcccggctg actccgcatt tctcgccaac gtcgcccaca gcgccgaaca
    89281 gtggcaactc aacgttgaaa agctcgatgc gcaagggatt atggcccgct ggccagaaat
    89341 acgcgtcccg gacaactaca tcggcttatt tgagactgat tccggttttt tgcgcagcga
    89401 actggcgatt aaaacctgga tccaactggc gaaggaagcg ggctgtgcgc aactgttcaa
    89461 ctgcccggtc accgcaattc gtcatgacga tgatggcgta actattgaaa cggttgacgg
    89521 tgagtatcag gcgaaaaaag cgattgtctg cgcgggaaca tgggtaaaag acctgctccc
    89581 ggagctgcct gtccagcccg tacgtaaagt atttgcctgg tatcaggccg atggccgcta
    89641 tagcgtgaag aataaattcc cggcgtttac cggtgaactg cccaatggcg atcaatatta
    89701 tggttttccg gcagaaaacg acgcgttgaa gattggcaaa cataacggag gccaggttat
    89761 ccattcagcg gatgaacgtg ttccgtttgc ggaagtggcc agtgatgggt cagaagcctt
    89821 cccgttcttg cgcaatgtat tgccgggtat cggttgctgc ctgtacggcg ctgcctgcac
    89881 ctatgataat tcgcctgacg aagattttat tatcgatacc ctacccggcc acgataatac
    89941 actgctcatt accggcctga gtgggcacgg ttttaaattt gcgtcagttt taggggaaat
    90001 agctgccgat tttgcgcaag accaaaaaag cgattttgat ttgacgccat tcaggctttc
    90061 ccgcttccaa taatcatcat tacggcctcc agaatgaggc cgtttttaca ggagtattta
    90121 atgcgtcggc tgttgcacta tctcatcaat aatattcgcg agcatctgat gctttatctt
    90181 tttctctggg gattgttggc aattatggat ctgatctatg tgttttattt ttagttttcc
    90241 gcactgacat aaactgacaa atatttaatc tccctttcat ttagcgtgaa aaattgaatg
    90301 agattattcc tgcttctaca gcactcacca gacatctctg atgctgaacc cactaaactt
    90361 atgaagattc tttcattatt aattcaaaat tattgaaata aaaataaata tcattcaatt
    90421 tgttattttt gtgttgcaat tcattctccg acacgccatt ttctttctct cgcaaattcg
    90481 tattggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt atatctgcgg
    90541 ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt ctgtggatgg
    90601 tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat aaaagcatcg
    90661 gtattttgtt aatgatgggg ctggttattc gcgttctgtg gcgtgtcata tctccaccgc
    90721 cgggtccgct gccgagttat tcgcccatga ctcgtcttgc cgcaaaagct ggacatctcg
    90781 cattgtgcct attgctgttc gccatcggca tcagcggcta tctgatctca actgccgatg
    90841 gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gacccttgcc gacgctggcg
    90901 cacaggcaga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc gtagtactgt
    90961 ctgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac gacactctga
    91021 agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa aaaagcctgc
    91081 ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc gccgattaca
    91141 aaattgacaa agaaggtcag cacgcctttg ttaacttccg catacagcat cttggctata
    91201 gctggttata tggcaccttt aaagatttcg acggtacttt tacctttgat gaaaaaaatc
    91261 cggctgccga taaagtgaat gtgacaatta acaccaccag cgtcgatact aaccacgccg
    91321 aacgcgataa acatcttcgc agtgcggatt ttctcaatac cacaaaatat ccacaggcaa
    91381 cattcacctc caccagcgtg aagaaagacg gtgacgaact ggatattacc ggcgatctga
    91441 cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt cagggcgacg
    91501 acccatgggg cggtaaacgt gcaggctttg aggccgaagg caaaattaag ctcaaagact
    91561 tcaatatcaa gacagattta ggtccagctt ctcaggaagt ggatctgatt atttcagtgg
    91621 aaggcgtaca gcagaagtaa gagtgaaatg ttgccggatg gcacgcatcc ggcaatgata
    91681 tttattctgt tggatcagga atacccagtg ttgtattcag acgtccacga gacttattaa
    91741 agatcttatt gccattttca cgtcctgccc gacggcgtcg ctgttcttct ggcggtaacg
    91801 cgctttcttc gcagcaaatc tcactacaac aacctttgta tttttccgcg catactggac
    91861 actgaataaa cagcaggtgg cagccatcat ttttacagtt ggtatggctg tcgcacggcg
    91921 cgccgcactg gtggcaatgc gcgataatct catccgatat acgttcgccc atccgctcgt
    91981 caaaaacaaa atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc gccttacggg
    92041 cgtattcaat aattccgccc tcgatatgcc agactttgtt gaatccgtta tgtttcatcc
    92101 aggcactggc cttttcacaa cgaatgccgc cggtgcagta catgacgatt tttttatctt
    92161 tatgtgcctg catcatctcg actgcttttg gcagctgctc acggaaggta tctgccggaa
    92221 tttcgagcgc gttttcaaag tgccccactt catactcata gtggttacgc atgtcgataa
    92281 atagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaga tactcaccaa
    92341 cattgctggc atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga tcgcgtacct
    92401 tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc aggcgtaaac
    92461 cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca ttgctcgcag
    92521 gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca aaaacattca
    92581 gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg atgtggaaat
    92641 acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc caacattttg gcttttaacg
    92701 cgtcgttgga aatgcggttg tgtaacactg gcatggtgta cggttcctgc gagatgggaa
    92761 agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca cattttacgc
    92821 tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg cccagtcttc
    92881 agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg cccgtatagg
    92941 ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact gcttcatccg
    93001 cgttattggt taacctggtt gggtattggc gtactttggt tggtcgtgca attgccctac
    93061 ccgattatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt tatgaaacga
    93121 cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag cgaacaagaa
    93181 cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggcctgat ggaaaccggt
    93241 atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat cggcatggaa
    93301 cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat ccattttctg
    93361 acactggagc tgggcgcgcg gcagtttggt atgcaggaac cggggattgg cgtttaccgc
    93421 ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg ctcaaataaa
    93481 tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa aggcgaagtg
    93541 gtctggtacg caccagatca tgattacggc ccgcgctcaa gcgttttcgt cccgttattt
    93601 gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat gtccggcgca
    93661 tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca attgattatg
    93721 ctgccaccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc gtggatgaac
    93781 aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt acaccgtcgc
    93841 tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccatgc cggatgcttc
    93901 agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa agctctctgg
    93961 attgcgcccc ctggaagtcg ggcgcataat tagtgtgttt atcttttctt cttatgttca
    94021 ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtgaaaatga cacccctata
    94081 aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg ggccgccttc
    94141 agtctggtaa tgcccttctt acccctctac gttgagcagc ttggtgttac cggccactcc
    94201 gccctgaata tgtggtccgg tattgtcttc agtataatgg gttcctaaac acatcatttt
    94261 aggtattgaa tatgagacgg gcaatcagct acatcagatt ttcaagtgaa cgacaactga
    94321 aaggggactc tgtacgccga caatctaagc tggttactga ctggcttgat aagaaccctg
    94381 agttttactt ggattcatcc ttgagcttta aagatttggg gaaaagtgct tttagtggta
    94441 agcacctgaa aggtggtcta ggtgactttt taaccgctat cgagaaaggg cttgttaaag
    94501 ctggtgatac gctactaatc gagagtttag accgtctgtc acgtcaggat attgatattg
    94561 cttctgagct attacgccgt atcttaaggg ctggtgtaga tgtagtcaca ctgagcgatg
    94621 gagaacatta cacacgagaa agtttaaaag acccgttagc cctgattaaa agcattctga
    94681 taatgcagcg ggcgcatgag gaaagtttga gaaagtctga gcgtgtccaa gctgcatgga
    94741 accgtaaaaa agaactcatc tccgaaggta tcaaggtatc taggcgttgt cctgcatggt
    94801 taaggctgaa tgatgaccgt cgaacattca caatcattcc tgataaagtg gaagttgtta
    94861 aacgagcatt tgacctgaga ttacagggtt taag
//
LOCUS       DAJTYM010000002        93437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTYM010000002 DAJTYM010000000
VERSION     DAJTYM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 93437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            100..225
                     /locus_tag="OO575_000107"
     ncRNA           100..225
                     /ncRNA_class="other"
                     /locus_tag="OO575_000107"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            345..686
                     /locus_tag="OO575_000108"
     CDS             345..686
                     /locus_tag="OO575_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6663218.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(677..1603)
                     /locus_tag="OO575_000109"
     CDS             complement(677..1603)
                     /locus_tag="OO575_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6663219.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1693..2691
                     /locus_tag="OO575_000110"
     CDS             1693..2691
                     /locus_tag="OO575_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6663220.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2688..2906)
                     /locus_tag="OO575_000111"
     CDS             complement(2688..2906)
                     /locus_tag="OO575_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6663221.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2908..4923)
                     /gene="ligA"
                     /locus_tag="OO575_000112"
     CDS             complement(2908..4923)
                     /gene="ligA"
                     /locus_tag="OO575_000112"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6663222.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4994..5980)
                     /gene="zipA"
                     /locus_tag="OO575_000113"
     CDS             complement(4994..5980)
                     /gene="zipA"
                     /locus_tag="OO575_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6663223.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6210..6971
                     /gene="cysZ"
                     /locus_tag="OO575_000114"
     CDS             6210..6971
                     /gene="cysZ"
                     /locus_tag="OO575_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6663224.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7156..8127
                     /gene="cysK"
                     /locus_tag="OO575_000115"
     CDS             7156..8127
                     /gene="cysK"
                     /locus_tag="OO575_000115"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6663225.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8511..8768
                     /gene="ptsH"
                     /locus_tag="OO575_000116"
     CDS             8511..8768
                     /gene="ptsH"
                     /locus_tag="OO575_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6663226.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8813..10540
                     /gene="ptsI"
                     /locus_tag="OO575_000117"
     CDS             8813..10540
                     /gene="ptsI"
                     /locus_tag="OO575_000117"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6663227.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10581..11090
                     /gene="crr"
                     /locus_tag="OO575_000118"
     CDS             10581..11090
                     /gene="crr"
                     /locus_tag="OO575_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6663228.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11132..11983)
                     /gene="pdxK"
                     /locus_tag="OO575_000119"
                     /gene_synonym="thiJ"
     CDS             complement(11132..11983)
                     /gene="pdxK"
                     /locus_tag="OO575_000119"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6663229.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12088..12462
                     /locus_tag="OO575_000120"
     CDS             12088..12462
                     /locus_tag="OO575_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6663230.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12495..13218
                     /locus_tag="OO575_000121"
                     /pseudo
     CDS             12495..13218
                     /locus_tag="OO575_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13422..14333)
                     /gene="cysM"
                     /locus_tag="OO575_000122"
     CDS             complement(13422..14333)
                     /gene="cysM"
                     /locus_tag="OO575_000122"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6663231.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14467..15564)
                     /gene="cysA"
                     /locus_tag="OO575_000123"
     CDS             complement(14467..15564)
                     /gene="cysA"
                     /locus_tag="OO575_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6663232.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15554..16429)
                     /gene="cysW"
                     /locus_tag="OO575_000124"
     CDS             complement(15554..16429)
                     /gene="cysW"
                     /locus_tag="OO575_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6663233.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16429..17262)
                     /gene="cysT"
                     /locus_tag="OO575_000125"
     CDS             complement(16429..17262)
                     /gene="cysT"
                     /locus_tag="OO575_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6663234.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17262..18278)
                     /gene="cysP"
                     /locus_tag="OO575_000126"
     CDS             complement(17262..18278)
                     /gene="cysP"
                     /locus_tag="OO575_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6663235.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18582..19373)
                     /gene="ucpA"
                     /locus_tag="OO575_000127"
     CDS             complement(18582..19373)
                     /gene="ucpA"
                     /locus_tag="OO575_000127"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6663236.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19560..20360)
                     /gene="murR"
                     /locus_tag="OO575_000128"
     CDS             complement(19560..20360)
                     /gene="murR"
                     /locus_tag="OO575_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6663237.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20524..21420
                     /gene="murQ"
                     /locus_tag="OO575_000129"
     CDS             20524..21420
                     /gene="murQ"
                     /locus_tag="OO575_000129"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6663238.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21424..22848
                     /gene="murP"
                     /locus_tag="OO575_000130"
     CDS             21424..22848
                     /gene="murP"
                     /locus_tag="OO575_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6663239.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(23028..23927)
                     /gene="yfeX"
                     /locus_tag="OO575_000131"
     CDS             complement(23028..23927)
                     /gene="yfeX"
                     /locus_tag="OO575_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6663240.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(24023..24598)
                     /locus_tag="OO575_000132"
     CDS             complement(24023..24598)
                     /locus_tag="OO575_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6663241.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24659..25108)
                     /locus_tag="OO575_000133"
     CDS             complement(24659..25108)
                     /locus_tag="OO575_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6663242.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(25095..25520)
                     /locus_tag="OO575_000134"
     CDS             complement(25095..25520)
                     /locus_tag="OO575_000134"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6663243.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25734..26603
                     /gene="amiA"
                     /locus_tag="OO575_000135"
     CDS             25734..26603
                     /gene="amiA"
                     /locus_tag="OO575_000135"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6663244.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26607..27506
                     /gene="hemF"
                     /locus_tag="OO575_000136"
     CDS             26607..27506
                     /gene="hemF"
                     /locus_tag="OO575_000136"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6663245.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27512..28564)
                     /gene="eutR"
                     /locus_tag="OO575_000137"
                     /pseudo
     CDS             complement(27512..28564)
                     /gene="eutR"
                     /locus_tag="OO575_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28610..29110)
                     /gene="eutK"
                     /locus_tag="OO575_000138"
     CDS             complement(28610..29110)
                     /gene="eutK"
                     /locus_tag="OO575_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6663246.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29123..29782)
                     /gene="eutL"
                     /locus_tag="OO575_000139"
     CDS             complement(29123..29782)
                     /gene="eutL"
                     /locus_tag="OO575_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6663247.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            30964..31755
                     /locus_tag="OO575_000140"
     CDS             30964..31755
                     /locus_tag="OO575_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663248.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32475..32870
                     /locus_tag="OO575_000141"
     CDS             32475..32870
                     /locus_tag="OO575_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6663249.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32888..33151
                     /locus_tag="OO575_000142"
     CDS             32888..33151
                     /locus_tag="OO575_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663250.1"
                     /translation="MHSLDSYFQRTTAPKSAAQEHREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            33174..33716
                     /locus_tag="OO575_000143"
     CDS             33174..33716
                     /locus_tag="OO575_000143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663251.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33855..34142
                     /locus_tag="OO575_000144"
     CDS             33855..34142
                     /locus_tag="OO575_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663252.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            34181..34387
                     /locus_tag="OO575_000145"
     CDS             34181..34387
                     /locus_tag="OO575_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663253.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34699..35184
                     /locus_tag="OO575_000146"
     CDS             34699..35184
                     /locus_tag="OO575_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6663254.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            35165..35443
                     /locus_tag="OO575_000147"
     CDS             35165..35443
                     /locus_tag="OO575_000147"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663255.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            35436..36896
                     /locus_tag="OO575_000148"
     CDS             35436..36896
                     /locus_tag="OO575_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6663256.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36930..37739
                     /gene="murI"
                     /locus_tag="OO575_000149"
     CDS             36930..37739
                     /gene="murI"
                     /locus_tag="OO575_000149"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6663257.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37858..38552)
                     /locus_tag="OO575_000150"
     CDS             complement(join(37858..38306,38306..38552))
                     /locus_tag="OO575_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6663258.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(39071..40444)
                     /gene="eutA"
                     /locus_tag="OO575_000151"
     CDS             complement(39071..40444)
                     /gene="eutA"
                     /locus_tag="OO575_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6663259.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40419..40898)
                     /gene="eutP"
                     /locus_tag="OO575_000152"
     CDS             complement(40419..40898)
                     /gene="eutP"
                     /locus_tag="OO575_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6663260.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40911..41246)
                     /gene="eutS"
                     /locus_tag="OO575_000153"
     CDS             complement(40911..41246)
                     /gene="eutS"
                     /locus_tag="OO575_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6663261.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(41539..43818)
                     /gene="maeB"
                     /locus_tag="OO575_000154"
     CDS             complement(41539..43818)
                     /gene="maeB"
                     /locus_tag="OO575_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6663262.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            44107..45057
                     /gene="tal"
                     /locus_tag="OO575_000155"
     CDS             44107..45057
                     /gene="tal"
                     /locus_tag="OO575_000155"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6663263.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            45077..47080
                     /gene="tkt"
                     /locus_tag="OO575_000156"
     CDS             45077..47080
                     /gene="tkt"
                     /locus_tag="OO575_000156"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6663264.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(47183..48226)
                     /locus_tag="OO575_000157"
     CDS             complement(47183..48226)
                     /locus_tag="OO575_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6663265.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSYCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(48352..48927)
                     /gene="nudK"
                     /locus_tag="OO575_000158"
     CDS             complement(48352..48927)
                     /gene="nudK"
                     /locus_tag="OO575_000158"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6663266.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(48995..50974)
                     /gene="aegA"
                     /locus_tag="OO575_000159"
     CDS             complement(48995..50974)
                     /gene="aegA"
                     /locus_tag="OO575_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6663267.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            51180..52897
                     /gene="narQ"
                     /locus_tag="OO575_000160"
                     /pseudo
     CDS             51180..52897
                     /gene="narQ"
                     /locus_tag="OO575_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            53099..56212
                     /gene="acrD"
                     /locus_tag="OO575_000161"
     CDS             53099..56212
                     /gene="acrD"
                     /locus_tag="OO575_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6663268.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(56311..56370)
                     /gene="ypfM"
                     /locus_tag="OO575_000162"
     CDS             complement(56311..56370)
                     /gene="ypfM"
                     /locus_tag="OO575_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6663269.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            56751..57107
                     /locus_tag="OO575_000163"
     CDS             56751..57107
                     /locus_tag="OO575_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6663270.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            57111..58238
                     /gene="dapE"
                     /locus_tag="OO575_000164"
     CDS             57111..58238
                     /gene="dapE"
                     /locus_tag="OO575_000164"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6663271.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            58266..58466
                     /locus_tag="OO575_000165"
     CDS             58266..58466
                     /locus_tag="OO575_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6663272.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(58576..59274)
                     /gene="ypfH"
                     /locus_tag="OO575_000166"
     CDS             complement(58576..59274)
                     /gene="ypfH"
                     /locus_tag="OO575_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6663273.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(59348..61363)
                     /gene="tmcA"
                     /locus_tag="OO575_000167"
     CDS             complement(59348..61363)
                     /gene="tmcA"
                     /locus_tag="OO575_000167"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6663274.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(61378..62241)
                     /locus_tag="OO575_000168"
     CDS             complement(61378..62241)
                     /locus_tag="OO575_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6663275.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(62409..63122)
                     /gene="purC"
                     /locus_tag="OO575_000169"
     CDS             complement(62409..63122)
                     /gene="purC"
                     /locus_tag="OO575_000169"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6663276.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIDLNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(63335..64369)
                     /gene="bamC"
                     /locus_tag="OO575_000170"
     CDS             complement(63335..64369)
                     /gene="bamC"
                     /locus_tag="OO575_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6663277.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(64386..65264)
                     /gene="dapA"
                     /locus_tag="OO575_000171"
     CDS             complement(64386..65264)
                     /gene="dapA"
                     /locus_tag="OO575_000171"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6663278.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            65410..65982
                     /locus_tag="OO575_000172"
     CDS             65410..65982
                     /locus_tag="OO575_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6663279.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            65982..66452
                     /gene="bcp"
                     /locus_tag="OO575_000173"
     CDS             65982..66452
                     /gene="bcp"
                     /locus_tag="OO575_000173"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6663280.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            66705..67322
                     /gene="hyfA"
                     /locus_tag="OO575_000174"
     CDS             66705..67322
                     /gene="hyfA"
                     /locus_tag="OO575_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6663281.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            67322..69339
                     /gene="hyfB"
                     /locus_tag="OO575_000175"
                     /pseudo
     CDS             67322..69339
                     /gene="hyfB"
                     /locus_tag="OO575_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            69350..70297
                     /gene="hyfC"
                     /locus_tag="OO575_000176"
     CDS             69350..70297
                     /gene="hyfC"
                     /locus_tag="OO575_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6663282.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            70314..71753
                     /gene="hyfD"
                     /locus_tag="OO575_000177"
     CDS             70314..71753
                     /gene="hyfD"
                     /locus_tag="OO575_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6663283.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            71765..72415
                     /gene="hyfE"
                     /locus_tag="OO575_000178"
     CDS             71765..72415
                     /gene="hyfE"
                     /locus_tag="OO575_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6663284.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            72420..74012
                     /gene="hyfF"
                     /locus_tag="OO575_000179"
     CDS             72420..74012
                     /gene="hyfF"
                     /locus_tag="OO575_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6663285.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            74002..75716
                     /gene="hyfG"
                     /locus_tag="OO575_000180"
                     /pseudo
     CDS             74002..75716
                     /gene="hyfG"
                     /locus_tag="OO575_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            75726..76271
                     /gene="hyfH"
                     /locus_tag="OO575_000181"
     CDS             75726..76271
                     /gene="hyfH"
                     /locus_tag="OO575_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6663286.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            76268..77026
                     /gene="hyfI"
                     /locus_tag="OO575_000182"
     CDS             76268..77026
                     /gene="hyfI"
                     /locus_tag="OO575_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6663287.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            77019..77432
                     /gene="hyfJ"
                     /locus_tag="OO575_000183"
     CDS             77019..77432
                     /gene="hyfJ"
                     /locus_tag="OO575_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6663288.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            77462..79291
                     /gene="hyfR"
                     /locus_tag="OO575_000184"
     CDS             77462..79291
                     /gene="hyfR"
                     /locus_tag="OO575_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6663289.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            79292..79474
                     /locus_tag="OO575_000185"
     CDS             79292..79474
                     /locus_tag="OO575_000185"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR6663290.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            79496..80368
                     /locus_tag="OO575_000186"
                     /pseudo
     CDS             79496..80368
                     /locus_tag="OO575_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(80462..81523)
                     /locus_tag="OO575_000187"
     CDS             complement(80462..81523)
                     /locus_tag="OO575_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6663291.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            81736..83199
                     /gene="bepA"
                     /locus_tag="OO575_000188"
     CDS             81736..83199
                     /gene="bepA"
                     /locus_tag="OO575_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6663292.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            83220..83579
                     /gene="arsC"
                     /locus_tag="OO575_000189"
     CDS             83220..83579
                     /gene="arsC"
                     /locus_tag="OO575_000189"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6663293.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(83712..84458)
                     /gene="hda"
                     /locus_tag="OO575_000190"
     CDS             complement(83712..84458)
                     /gene="hda"
                     /locus_tag="OO575_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6663294.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(84508..85797)
                     /gene="uraA"
                     /locus_tag="OO575_000191"
     CDS             complement(84508..85797)
                     /gene="uraA"
                     /locus_tag="OO575_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6663295.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(85883..86509)
                     /gene="upp"
                     /locus_tag="OO575_000192"
     CDS             complement(85883..86509)
                     /gene="upp"
                     /locus_tag="OO575_000192"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6663296.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            86833..87870
                     /gene="purM"
                     /locus_tag="OO575_000193"
     CDS             86833..87870
                     /gene="purM"
                     /locus_tag="OO575_000193"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6663297.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            87870..88508
                     /gene="purN"
                     /locus_tag="OO575_000194"
     CDS             87870..88508
                     /gene="purN"
                     /locus_tag="OO575_000194"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6663298.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            88680..90746
                     /gene="ppk1"
                     /locus_tag="OO575_000195"
     CDS             88680..90746
                     /gene="ppk1"
                     /locus_tag="OO575_000195"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6663299.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            90751..92292
                     /gene="ppx"
                     /locus_tag="OO575_000196"
     CDS             90751..92292
                     /gene="ppx"
                     /locus_tag="OO575_000196"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6663300.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(92331..>93437)
                     /gene="pdeF"
                     /locus_tag="OO575_000197"
     CDS             complement(92331..>93437)
                     /gene="pdeF"
                     /locus_tag="OO575_000197"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6663301.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        22615 a        24391 c        23552 g        22879 t
ORIGIN      
        1 tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac
       61 gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg atactcaaag
      121 attcgaacct gcagcaggtt tgagttgagc gcagcgaaac aacggagccg ctcgcggcga
      181 cggcccgaag ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgca aaaaagcgcc
      241 ctaaaggcgc ttttttgcta tctgcgattt gtaagattga tttttcttct caagttcctt
      301 gttcttctac cgatattacc tcgtacagtg acgaggactg aacaatggcc actatctctt
      361 caacttcaat tccttccata caaacacaga gtagtaacag ggcatcacaa ggtagcgatg
      421 ttgcttcaca aatcgcgcgg ataagtcagc aaatcatcaa actcactcaa caaattaaag
      481 agattgtaga cactagcggc agtgctgaag acaagcaaaa acaggccgaa ttgattcaac
      541 agcaaatcac cctactggaa acacaactgg cgcagttgca gaaacagcag gcagaaaagg
      601 cgcaggagaa agagcagcgt ttgtctctga atgtatccct tctaaatcct gttgaaaaaa
      661 cgactcatat cgatatctat atctgataca acggatcccc ttcccgacca gccattagcg
      721 cattgccctg gtctcgtacc acgtcccata acgcttcagc ggcggtggaa agtgaacggt
      781 tcttcctgcg caccagcatc aactggcgtt caaccaccgg cgtaatgcgc ttaaccacca
      841 gtgggctacc ttcaggcaat ggtagcgcca gtgcaggaag aatactgatg ccgatgcctg
      901 cggctaccat cgggaacagc gtcgccggat gtccaatctc ctgcacaata ttcgcctgaa
      961 taccattgcg cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg taatcctgca
     1021 acaccaattt cgctccctgt aatgcctgcc agggtacata atcttccacg gccaaagcgc
     1081 tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat cgcttcacat tgcagatcgc
     1141 ccactgggcc tggatcgatg acgatgccaa agtcgacatc tccctggcga atactttcca
     1201 tcacccactg ctgcggacga tcatgcagga caaactgaat atctggatag cggcgatggc
     1261 tttcagcaat gcattgcgga ataagatgcg cggaaatggt ctggctggca gcgacccgaa
     1321 cttttccgct cagttgttgc cccatacgcc cggtatcgcg caacgtgctg ttaagttcat
     1381 ccagcagtcg ctcaagacgc aaagccaact gctgccctgc atctgtaagc accacttcac
     1441 gcgtggttct gtccagcagg cgaacaccag tatgattttc cagctccttc acactgtgac
     1501 ttactgccga ctggctcaag ccgatacgct ctcctgcacg actaaaactt ttctcctgcg
     1561 ctactgtgac gaaaaccttt aattgtttta aagaataatt catctgtttt cttcatgaat
     1621 ggatgcaata aatcaatttt atttctcaaa ctggaagaag cacaatagaa ccatcgatca
     1681 tctggagtct ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac cctgatcacg
     1741 gtggtgttgc tggcctcttt ctttccggcc agaggcgatt tcgtcccctt ctttgaaaat
     1801 ctgaccaccg cagctattgc cctgctgttc tttatgcacg gcgcgaagtt gtcgcgtgag
     1861 gcgattattg ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg cagcaccttc
     1921 gtgctgtttc cgattctggg tgtactgttt gcctggtgga aaccggtaaa tgtcgacccg
     1981 atgctctact ccggttttct ctacttgtgc attctcccgg ctaccgtgca gtctgcaatc
     2041 gccttcacgt caatggcggg cggtaacgtc gcggcggcgg tttgttctgc gtcagcatcc
     2101 agcctgctgg ggattttcct ttcaccattg ctggttggtc tggtgatgaa tgttcacggt
     2161 gcaaggggca gccttgagca ggtcggtaaa attatgctgc aactgctgct gccgtttgtg
     2221 ttggggcatc tttcccggcc gtggattggt gactgggtgt cgcgcaataa aaaatggatt
     2281 gcgaaaactg accagacgtc cattctgttg gtggtttata cagcgttcag cgaagccgtc
     2341 gttaatggta tctggcataa agttggctgg ggatcattgc tgtttatcgt ggtggtcagc
     2401 tgcgttcttc tggctatcgt gattgtagtt aacgtcttta tggcacgccg actgagcttc
     2461 aataaggcag atgaaattac tatcgtcttt tgtggttcga aaaagagtct ggcaaatggc
     2521 atcccgatgg caaacattct gttccccaca tcggtgatcg gtatgatggt gctgcccctg
     2581 atgattttcc atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg atacaaacgc
     2641 cagaccgaac agttacaggc gcagcaggaa agcagcgccg ataaagctta aagcggacgc
     2701 ttcaggggct ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt gatttgcatt
     2761 agctcaccga gcttccctgc cgggaactca tctttgcggg caaaccacag cagatactcc
     2821 tccggcaggt caattaagcg acgccctttg tatttgccaa acggcattat cgtattggct
     2881 atttcaatca gctgctcttt ttccatctca gctacccagc aaacgcagca tttccgcttc
     2941 gtcgatgact tcaatgccca gttcctgcgc cttcgccagt ttagatcctg cagcttcacc
     3001 cgctatcacc agatcggttt tcttcgacac gctgcccgcg actttcgcgc ccagttcgac
     3061 cagtcgagct ttagcgtcat cacgcgacat ctggcttaag ctgcccgtaa gcaccacggt
     3121 tttaccagca aacgggctgt caatctcttc cgcgttgata acgatcggcg caggccagtg
     3181 aacaccttcc gccaacagct cgctgatgac attgcggttg ctttcttcgg caaagaagtt
     3241 gtgaacgtgg gatgcaacga caatgccaac atcaggcacc ttttgcagct cttcaatcga
     3301 agcggcttcc agcgcttcca gcgtgccgaa atatgccgcc agacctgctg cggtggcctc
     3361 gccgacttca cggatgccaa gtgcatagag gaagcgagca aaggtggttt ctttcgcttt
     3421 ttccagcgcg ttaaccacgt tttgtgccaa ttttggcccc atacgctcca gtccggtcag
     3481 tttgcctgcg gtgagtttga acagatctgc cggagtatgg acatattctt tttcaaccag
     3541 ctgatcgatg attttgtcgc ccattccgtc aacatccatc gcacggcggg aaacaaagtg
     3601 tttcagcgac tctttacgct gcgcaccgca aatcaggcca ccggtacagc gggcaaccgc
     3661 ttcaccttcc acacgctcaa cgtcagaacc acataccgga caatgcgtcg ggaatacaac
     3721 ctcacgggta tcttccgggc gttcagaaag cacgacgtta accacctgcg ggatcacgtc
     3781 gccagcgcgg cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt cgatttcatc
     3841 cgcattgtgt aaggttgcgt tactcaccag cacgccagca acatggacag gttccagacg
     3901 cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac gcacaaaggt
     3961 catctgctcc tgcgccggga atttaaacgc aaccgcccag cgcggggcac gcgcgacaaa
     4021 gccaagctgc tcctgctgtt ccagtgagtt gaccttaatc accacgccgt cgatatcaaa
     4081 gcccagcgtc gggcggtctt cttccacttt gtgatagaac gccagcactt cttccgccga
     4141 ttcacaaagc gttacccgat cgctgaccgg caccccccac tttttaaatt gcagtaaacg
     4201 gccaagatga gtatccggca gctcgccacc ttccagaaca ccaacgccat agcagaaaaa
     4261 agtgagcggt cgcttcgctg taatacgcgg atcaagctga cgcagtgaac cagctgccgc
     4321 attacgtggg ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt taatcttttc
     4381 gaaccccgcc tgcggcagga acacttcacc acgcacttcc agacgcgccg ggatattctc
     4441 tccgtgcagc ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga tatcttcccc
     4501 ggtggtgcca tcgccacggg tcgcggcact gactaaaacg ccattttcat acagaatact
     4561 gacggcaagg ccatccagct tcagctcaca gcaccaggtg actttctcgt tgtttttcag
     4621 acggtcctgc acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa cgttatccag
     4681 tgacagcatt ggtacttcat ggcgtatctg gctgaaagct gccagcggcg cagcacctac
     4741 acgttgggta ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca gctcgcgcag
     4801 ttcgcgcatc agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca tcacatgata
     4861 aagatattca tgatggcaaa gcgtcgttcg cagttctgtc agttgttgtt cgattgattc
     4921 catatcgcac catcaatgct aaaaaccccc gacaagcggg ggttcgaaga ggagttaatt
     4981 tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc tggtactcgc
     5041 gcaatttctg cggagtcatc atacggcgct gatcgtcaag cacgacaccg cccacttcat
     5101 cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg tcaccgtaag
     5161 acggtacctg cataaagata gtgacacccg gagtagtgaa atccttcatt tcaggatcaa
     5221 aggttcccgg tttcaccata ttcgccaggc tgaataacgc cgggccgctg ccatccgggc
     5281 taagatgacg atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt tgaatgctgt
     5341 taagaagcag ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc atgataatca
     5401 ccgcttcttt gcgcttcggt ttatccataa ctggagccgg ttccgctaca ggctcaggct
     5461 gtggtgccgc tacgggttct gcaggctgga aagcctgttg tgccggttgc ggtgctgaat
     5521 gcacaggctg cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac ggctgttcag
     5581 gctgcggctg ataggcaggc tgctgcaccg gctgcgctgg acgcggagca tgttgcggcg
     5641 gtacctgcgc ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg
     5701 gtaggtactg gtgttgcggc gacggacgag cagcctcatg ctcctgagcg ttagccgggg
     5761 catgattcac gcggtgaaca cgaacctcac caacgccctc atcatcttcg acatcctcgt
     5821 cataagaatc gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg cggaacatag
     5881 aagatcgttc tttacggctg gtccagaaac catgtaccag taaagcgatt atggcgatcg
     5941 cgccaacaat gattaatatc agacgcaaat cctgcatcat tatattctct gttgttctaa
     6001 caccttgcca ccacggcaaa catttactca ctaagagtat ttgccgatta cctcaagtgc
     6061 aagtgcacta ttaactttca cagcacaaag atagatgaaa tcgtgctttt tgctgttttt
     6121 tcgaacatat cctaactgtc cattgcgcaa ttacccggta aaatacgcag aattttcctg
     6181 ggattggtca aaaggagctc atcctgatta tggtttcatc attcacatct gccccacgca
     6241 gcggttttta ctattttgcg caaggctgga agctcgtctc gcaacctggg attcggcgtt
     6301 tcgttatttt accgctgctg gtcaatattt tgttgatggg gggcgcattc tggtggctct
     6361 ttacacagct cgatgtctgg atcccgactt tcatgagtta cgttccggac tggctacaat
     6421 ggctgagtta tctgttgtgg cctctggctg tcatctctgt gctgttagtg tttggctatt
     6481 tcttctccac gattgctaac tggattgccg ctccgtttaa cggtttattg gctgaacaac
     6541 tggaagcacg attgactggc gctacaccgc cagataccgg gattttcggt atcatgaaag
     6601 atgtgccgcg aatcatgaaa cgcgaatggc aaaaatttgt ctggtatctg ccgcgcgcaa
     6661 ttgtattact aattctttac ttcatccctg gtattgggca aaccgtcgcg ccggtactgt
     6721 ggttcctgtt tagcgcctgg atgttagcca tccagtattg cgattacccc ttcgataacc
     6781 acaaagtgcc gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc
     6841 agtttggtgc tttaaccagc ctgtttacga tgatcccgct gcttaatctg ttcatcatgc
     6901 ccgttgccgt ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat aaacacgcga
     6961 tgtggcggta acaatctacc ggttattttg taaaccgttt gtgtgaaaca ggggtggctt
     7021 atgccgcccc ttattccatc ttgcatgtca ttatttccct tctgtatata gatatgctaa
     7081 atccttactt ccgcatattc tctgagcggg tatgctacct gttgtatccc aatttcatac
     7141 agttaaggac aggccatgag taagattttt gaagataact cgctgactat cggtcacacg
     7201 ccgctggttc gcctgaatcg catcggtaac ggacgcattc tggcgaaggt ggaatctcgt
     7261 aaccccagct tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag
     7321 cgcggcgtgc tgaaaccagg cgttgaactg gttgaaccga ccagcggtaa taccgggatt
     7381 gcactggcct atgtagctgc cgctcgcggt tacaaactca ccctgaccat gccagaaacc
     7441 atgagtattg aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt gctgacggaa
     7501 ggtgctaaag gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca
     7561 gagaaatacc tgctgctgca acaattcagc aatccggcaa accctgaaat tcacgaaaaa
     7621 accaccggtc cggagatctg ggaagatacc gacggtcagg ttgatgtatt tattgctggc
     7681 gttgggactg gcggtacgct gactggcgtc agccgctaca ttaaaggcac caaaggcaag
     7741 accgatctta tctctgtcgc cgttgagcca accgattctc cagttatcgc ccaggtgctg
     7801 gcaggtgaag agattaaacc tggcccgcat aaaattcagg gtattggcgc tggttttatc
     7861 ccggctaacc tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc
     7921 atttctaccg cccgtcgtct aatggaagaa gaaggtattc ttgcaggtat ctcttctgga
     7981 gcagctgttg ccgcagcgtt gaaactacaa gaagatgaaa gctttaccaa caagaatatt
     8041 gtggttattc taccatcatc gggtgagcgt tatttaagca ccgcattgtt tgccgatctc
     8101 ttcactgaga aagaattgca acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc
     8161 tttttaggtg cttttttgtg gcctgcttca aactttcgcc cctcctggca ttgattcagc
     8221 ctgtcggaac tggtatttaa ccagactaat tattttgatg cgcgaaatta atcgttacag
     8281 gaaaagccaa agctgaatcg attttatgat ttggttcaat tcttccttta gcggcataat
     8341 gtttaatgac gtacgaaacg tcagcggtca acacccgcca gcaatggact gtattgcgct
     8401 cttcgtgcgt cgcgtctgtt aaaaactggc gctaacaata caggctaaag tcgaaccgcc
     8461 aggctagact ttagttccac aacactaaac ctataagttg gggaaataca atgttccagc
     8521 aagaagttac cattaccgct ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa
     8581 aagaagctaa gggcttcact tctgaaatta ctgtgacttc caacggcaaa agcgccagcg
     8641 cgaaaagcct gtttaaactg cagactctgg gcctgactca aggtaccgtt gtgactatct
     8701 ccgcagaagg cgaagacgag cagaaagcgg ttgaacatct ggttaaactg atggcggaac
     8761 tcgagtaatt tcccgggttc ttttaaaaat cagtcacaag taaggtaggg ttatgatttc
     8821 aggcatttta gcatccccgg gtatcgcttt cggtaaagct ctgcttctga aagaagacga
     8881 aattgtcatt gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt
     8941 tctgagcggt cgtgccaagg catcagccca gctggaaacg atcaaaacga aagctggtga
     9001 aacgttcggt gaagaaaaag aagccatctt tgaagggcat attatgctgc tcgaagatga
     9061 ggagctggag caggaaatca tagccctgat taaagataaa cacatgacag ctgacgcagc
     9121 tgctcatgaa gttatcgaag gtcaggcttc tgccctggaa gagctggatg atgaatacct
     9181 gaaagaacgt gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca acatcctggg
     9241 cctgaagatt atcgacctga gcgccattca ggatgaagtc attctggttg ccgctgacct
     9301 gacgccgtcc gaaaccgcac agctgaacct gaagaaggtg ctgggtttca tcaccgacgc
     9361 gggtggccgt acttcccaca cctctatcat ggcgcgttct ctggaactgc ctgctatcgt
     9421 gggtaccggt agcgtcacct ctcaggtgaa aaatgacgac tatctgattc tggatgccgt
     9481 aaataatcag gtttacgtca atccaaccaa cgaagttatt gataaaatgc gcgctgttca
     9541 ggagcaagtg gcttctgaaa aagcagagct tgctaaactg aaagatctgc cagctattac
     9601 gctggacggt caccaggtag aagtatgcgc taacattggt acggttcgtg acgttgaagg
     9661 tgcagagcgt aacggcgctg aaggcgttgg tctgtatcgt actgagttcc tgttcatgga
     9721 ccgcgacgcg ctgcccactg aagaagaaca gtttgctgct tacaaagcag tggctgaagc
     9781 gtgtggctcg caggcggtta tcgttcgtac catggacatc ggcggcgaca aagagctgcc
     9841 atacatgaac ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc
     9901 gatggatcgt aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg cctcggcttt
     9961 cggtaaattg cgcattatgt tcccgatgat catctctgtt gaagaagtgc gtgcactgcg
    10021 caaagagatc gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc
    10081 aattgaaatc ggcgtaatgg tggaaacacc ggctgccgca acaattgcac gtcatttagc
    10141 caaagaagtt gatttcttta gtatcggcac caatgattta acgcagtaca ctctggcagt
    10201 tgaccgtggt aatgatatga tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa
    10261 cttgatcaag caagttattg atgcttctca tgctgaaggc aaatggactg gcatgtgtgg
    10321 tgagcttgct ggcgatgaac gtgctacact tctgttgctg gggatgggtc tggacgaatt
    10381 ctctatgagc gccatttcta tcccgcgcat taagaagatt atccgtaaca cgaacttcga
    10441 agatgcgaag gtgttagcag agcaggctct tgctcaaccg acaacggacg agttaatgac
    10501 gctggttaac aagttcattg aagaaaaaac aatctgctaa tccacgagat gcggcccaat
    10561 ttactgctta ggagaagatc atgggtttgt tcgataaact gaaatctctg gtttccgacg
    10621 acaaaaagga taccggaact attgagatca ttgctccgct ctctggcgag atcgtcaata
    10681 tcgaagacgt gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt attgctatca
    10741 aaccaacggg taacaaaatg gtcgcgcctg tagacggtac cattggtaaa atctttgaaa
    10801 ccaatcacgc gttctctatc gaatctgata gcggcgttga actgttcgtc cacttcggta
    10861 tcgacaccgt tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg
    10921 tgaaagttgg cgatactgtc attgaatttg atctgccgct gctggaagag aaagccaagt
    10981 ctaccctgac tccggttgtt atctccaaca tggacgaaat caaagaactg atcaaactgt
    11041 ccggtagcgt aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc
    11101 agtgaaaaat ggcgcccatc ggcgccattt tttatgcttc cgccagcggc ggcaaaatca
    11161 attcatcgct ctcatgctgc tgggtgtagc gcatcacttc cagtacgcgc aaccccgcac
    11221 ggtgcaccgc atcgtttaac gccttccctt tcagcaaacc actgataagc tgagcacaaa
    11281 acaggtcgcc agtccctttc aggtcggttt ttacccgtga atgggaaatg acattcacgc
    11341 tgtcggcact gaccaccacc acctgcatct cctgattttc ttcattaccg gaggcgctgg
    11401 taatcaccac ccattttaat gtgtctgaaa gcagactttt tgcggcagca atggcactgt
    11461 cgagatcgcg gcaattttta ccggtcagga tttccagctc aaagatattg ggggtaattc
    11521 cctgcgccag cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt ttgacataaa
    11581 ttccgctatc aatatcgcca atcaccggat cgaccatgat caataggtca ggatggtctt
    11641 tgcgtagcgc agtcagccac tcggcaagga ttttgatttg cgatgccgtt cccatatagc
    11701 ccgtggttac agcacgaagt tggcgcagcg catcacgctc ctgaagcgca cgcaaatagc
    11761 cgctaaacca ttcgtccgga atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc
    11821 tcagcaatac cgtcggcacg gcaaagacat tcaagccgtt ctgttttata gcaggcaccg
    11881 caatgctgtt gcctacgctt ccgtacacta cctgcgactg cacggcgacg atatccgcct
    11941 gcagcgccct actcttatcg ttaaacaaca acaaactact catttaattt tttctccttg
    12001 ccgatgatcc tcatcgtatt ccaaccgaaa ctttacctga ttctggcagt caaatcggct
    12061 attacaaaac aaagataagg taattcagtg aagaaaatca tttgtctggc cattacgctg
    12121 ctaatgacac tccccgctta cgcgaagtta actgttcatg aagaagcccg catcaacgcc
    12181 atgctggagg gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg tgatgaacat
    12241 acctgcgatg aagcggtttc ccacctgcgt ctgaagctcg gcaatacccg taaccgcatt
    12301 gacactgccg agcagtttat tgataaggta gcttcgtcgt catcgattac cgggaagccg
    12361 tatatcgtga agatccccgg taagagcgat gagaacgcac agcctttttt acatgcgtta
    12421 attgcgcaga cggataaaac ggtgcctgcg gaaggaaatt aatccgcttt ggtaaggcat
    12481 ttacaggagg taacatgaaa aaatgcttta tttatcacga tgaaaaatcg aataaatttt
    12541 ggtggataga ttacgaaggg gatagtttag ctgtcaacta tggcaaggta ggtagtattg
    12601 gtaaattcca tacaaaagaa ttcgataatg aagaacagtg tctgaaagaa gccagtaaat
    12661 tgattgccgc aaaaatgaaa aaaggatatc aagaaactcc agactttaac ttcatggatc
    12721 tctactattt tgatgatgaa gagattgggt tacatcttaa aacgtcacac ccaaacttcc
    12781 agtgccattt tactgatcca ctttatatgt gttgctggga tgaagaatct ccttttggta
    12841 gcgatgaagg tgctgatgct ctaaacgaac ttgaaaatag cctccgttct ggactgtgct
    12901 gatttccctc aaatgttaat tgaaactatg tggggtatga aatacatcgc tatggacagt
    12961 attcttgaag aggatgtgcg tgcgcaatta ctagccgatg aaatgagctc tatccagagc
    13021 aatatgatta cctacgcgac tgcattcggt cagatcaaag tcatgggtaa aatctcccat
    13081 aaacttaaaa agatgggact caatgcacta gcgcgtcatc agcttaccgc aaaaattctt
    13141 caatggggtg acggtcagga ctcaccaata cttcaaaaaa tgattgatga ccttactgct
    13201 tttccacacg aaaattaaaa actgcatttg tcggcagcaa caactgccga caaatcagat
    13261 tattgacaaa gtgcactttg tttatgccgg atgcggcgta aacgcctgat ccggcataaa
    13321 aatattgcta attcaatata ttgcagaaac attgtaggcc tgataagcgg agcgcatcag
    13381 gcagttttgc gtttgtcatc agtctccaat gctattaatc cttaaatccc tgccccctga
    13441 ctaaaatgct cttccccaaa cactccggtg gagaggtagc gatcgccacg atcgcagatg
    13501 atcgctacca ccaccgcacc agggttagct tttgccaccc gcagtgctcc agcaaccgcg
    13561 ccgcctgagc tgacgccaca gaatattcct tcccgcaccg ccagttcgcg catggtgttt
    13621 tccgcatcgc gctgatgaat atccagcacc tcatccacca gcgcagcgtt gaaaatcccc
    13681 ggcagatatt ccgcaggcca gcggcgaatg cccggaatac tgctgccttc ttccggttgc
    13741 aggccaacaa tggtcaccgg tttggattgt tcgcgcataa agcatgaaac gccggtgata
    13801 gtgccggtcg tccccatgct ggagacaaaa tgagtgatgc gcccgccggt ttgctgccag
    13861 atttccggcc cggtggtggt gtaatgcgca taagggttat cgggattatt gaactgatcg
    13921 agcagctttc cttcgccacg attcgccatc tccagcgcca gatcgcgcgc accttccatg
    13981 ccctgctctt tggtgacaag aatcagttcc gcaccataag cacgcatcgc cgcacggcgt
    14041 tcctggctca tgttgtcggg catcagcaat ttcatgcgat agcctttcag cgcggcaatc
    14101 attgccagcg caatgccggt gttaccactg gtggcttcga ttaagacatc acccggttta
    14161 atttccccgc gcttttccgc ctcgacgatc atcgaaagtg ccgcacgatc tttcaccgaa
    14221 cctgccgggt tattgccttc cagttttaac cacacttcac tgccgttatc cggccccatt
    14281 cgctgcaact tcaccagagg cgtattgcct attgtttgtt ctaatgtact cacaatctct
    14341 atccatacgt ggtgttgcct gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca
    14401 aaccttgcca ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta acattcactc
    14461 aacctatcag gcgctttgtg cgagagcaag ttcctcatcg cgggtttcaa tacgctcgtc
    14521 gccgttatac agccgcgcat gttgcagacc aacgaataaa cgctcgccat gctgcggggc
    14581 atcgtcgcca tgcatcacga ccgtcagcgg ttcgttgtac caccccagcg gctgcaccac
    14641 taattgggtg tagtgacctt tcgggctggc ttccagtacc tgtaccggca gcggcgaatc
    14701 gagactggta cggcggctga tatccacttc ccacgggcgc aggaagagat ccaccggccc
    14761 ctgatacgca ggtgtgtagc ccagcggcca gcgatgcgcg ccaacatgga actgcccgcc
    14821 gcgaatggtt ccctgcaggc ggttcacttc gcccataaat tcgagtacaa aacgggtcgc
    14881 tggttcgcgc catacctgat tcggcgcatc agcctgttca atattcccct gactcatcac
    14941 cactacacga tcggctactt cggtcgcctc ttcctggtcg tgggtcacaa acacgctggt
    15001 gaatttcagc tcttcatgca gttgacgcag ccagcgacgc agctctttac gcacctgcgc
    15061 atccagcgcg ccgaacggtt catcaagcag cagaatttgc ggttctacag ccagcgcgcg
    15121 cgccagcgcc acacgctgtt tctggccgcc ggaaagctgc gccggataac gatccgccag
    15181 atgcgcaagc tggaccattt ccagcaattt tgtcactttc gctttgatgg ctgcggcatt
    15241 cgggcgctcg cgacgcggca gcaccgtcag gccaaaagtg atattgtcga ataccgtcat
    15301 atggcggaac agcgcgtaat gctggaacac gaaaccgact ttacgatcgc gcgcgtgcag
    15361 gcgactcacg tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct ccagcccggc
    15421 gataatccgc agcagcgtgg ttttcccgga accggacggc cccagcaacg cgaccatctg
    15481 accagaagga atatccagtg agatatcgtt cagcacctgg gtgcgaccaa aagacttctt
    15541 gatattggca atctcaatgc tcatgatgct cctcctgctg cgcgcgtttt tcctgattct
    15601 ccaggcgcca ctgcaacata ctctttaaaa acagggtgat aatcgccatc aacgtcaaca
    15661 gcgcggcggc ggtaaaggag ccgacggtgt tgtagtcctg ctccaacaat tcaatctgta
    15721 acggcagcga aagtgtttcg ccgcgaatcg agccggaaac caccgacact gcaccaaact
    15781 cgccaatcgc gcgggcgttg gtcagcacca cgccgtaaag cagcgcccag cgaatattcg
    15841 gcaatgtgac gcgacggaac atctgccagc cggacgcgcc aagcaaaatc gccgcttcgt
    15901 cttcctggct gccctggctt aacatcaccg gcaccagctc gcgcaccaca aacggacacg
    15961 tcacaaagat ggtgaccagc accattccgg gccaggagaa cataatttgc agattatgtt
    16021 catccagcca gccgccgagc gggccgttgg agccgtagaa cagcagatac accagaccgg
    16081 caaccaccgg cgatacagca aacggaatgt ccagtagcgt cagcagtaac tggcgtcccg
    16141 ggaagttaaa gcgcgtcacc agccaggcca gcagaatgcc gaacaccagg tttaccggta
    16201 cggcaatcag cgcgatcatc accgtcagcc agatggcgtg cagcatgtcc ggatcggcca
    16261 gattctgtaa aaccggcatc agccccttgc tgaatgcctg cacgaagatg taaatcatcg
    16321 gcaccagcag gatgaacgcc gaaaccagca tcccgatgcc aatcagaaac catttgcccc
    16381 agttaatcgg gcgcgcgtca taacgcttca attgggtaac ttccgccatt aatgacctac
    16441 cacacgccga ccaaagcgac tttgcagagt gttaattgag aacagcagca gcagagatgc
    16501 cgcgaggatc accgaagcaa tcgcgctcgc tgccgggtaa tcaaactcct gtaagcgcac
    16561 aaaaatcatc agcgacgtca cttccgtctt ccacgcgata tttccggcga taaaaatcac
    16621 cgcgccaaat tcaccaagac tacgggtaaa cgacagcgcc acgcccgcca ccagcgccgg
    16681 agaaagctcc ggcagcacca ctttgcagaa actctgccag cgcgttgcac caagcgtttc
    16741 cgccgcttct tcatattccg ggcctaactc ttccagcacc ggctgcacgg tacgcaccac
    16801 aaacggaatg ctggtaaagg ccatcgccac cgcaatacca agccaggtat aggtgacttt
    16861 gatatcaaac ttcgccagcc attcaccgta aaaaccgttt acggaaaaga gcgaggccag
    16921 cgttaaaccg gcgactgccg ttggcagcgc aaagggtaaa tccatcagcg catcaagcag
    16981 cgtgcggccc ggaaagcgat aacgtgttaa aatccacgcc atcagcagac cgaaaacgcc
    17041 gttaaaaatc gatgccacaa acgccgacag cagcgtcact ttgtaggccg cgaccacctg
    17101 cgggttggtg atcacctccc agtactgcgc ccagctcatc tcagaaagct gcatcaccag
    17161 cgcagagagc ggcagcagca atatcaggca cacaaacagc agactggtgc caaggcttaa
    17221 agtaaagccc ggcagcacgc gtcttgaaga aactgcaaac attacttacg ccccgccgct
    17281 aacagcttgt ctaactcgcc gccgctggtg aagtgggttt tcatcacttc cggccatgag
    17341 ccaaatttgt cttccacgcg gaacagttcg gtctgcggga atttatcttt cagtttgtcc
    17401 atgacttccg ggttattcac acggtagtaa tagtcggtga tgatggtctg cgcctgtggg
    17461 ctgtagagcc agttcaggta ggcttttgcg gctttttccg taccgttggc ctgcacattt
    17521 ttatcgaccc acgccaccgg gaattccgcc agaatattgg ttttcgggat caccacttca
    17581 aagccctgtg cttcatactg tttacggatg ttgttcactt ccgattcaaa gctaatcagc
    17641 acatcgccca agccacgctc ggcaaaagtg gtggttgcgc cacgaccgcc ggtatcgaac
    17701 acttcaacgt ttttcaggaa ctgggtcata aactgttcgg ttttggcttt atcgccaccg
    17761 tcagctttat ccgctgcgcc ccatgccgcc agataggtat aacgcgcgtt acccgacgtt
    17821 ttcgggttcg ggaaaatcag cttcacgtcg gagcgcacca ggtcgttcca gtcgtggata
    17881 ttcttcgggt tgcccttacg caccaggaag cccatggtgg agtagaacgg cgagctatta
    17941 ttcggcaggc gcgactgcca gtcagccggg atcagcttgc ctttgtcgtg caggatttgt
    18001 acgtcggtca cctggttata agtgacaacg tctgctttta agccctgcaa aatcgccagc
    18061 gcctgttttg atgacccggc atgagattgt tttatcgtca gtttgtcgcc gccgttatct
    18121 tttgcccatt gctgttcaaa cggcggattc agggcggcaa acagctcgcg ggagacgtca
    18181 taagaactgt tcagcagttc cgttgcctgt acatggcccg ccagcagcag agaagcgacc
    18241 agcgcgagtg agttcttttt cagtaagtta acggccattg cgcaccctta taaatttaat
    18301 gactttctaa tagccatcat atttataacg gacgtgaaag gagtaacggt tttatatacc
    18361 gtttggtgat ttggaagtta aaaagggaat aagaacttgg ttcaacaggc gtaattgttg
    18421 caggcagttt ggacacggac agcgcggaga aaccggagcg tacacatagt acgtgaggat
    18481 ttcgagcact gcccggggcc aaaatgacaa atgaaatagc ctggtgaact tagttcaaga
    18541 cgaaatcctc cccacacatg cagggaggaa acagaggtga atcagatacc gacgctaacc
    18601 gtctccggca gtgtgctgcc gccatcaatc acattctgtg taccggttaa atagctggat
    18661 tcatccgatg cgaggaaggc cgccagttcg ccgacttcca gcggatcggc gaggcgacac
    18721 agcgggattg cttttgccat ttcagtcagc accgattctg gatcttccgg gttcgactgg
    18781 cgggcaatgc tttccgccat tggcgtgcgg acgtatcccg ggcagatggc gttaacgcga
    18841 ataccggatt gcgcgtactc caccgccagc gatttagtca ggccaacaat cgccgctttc
    18901 gttaaggcat acgccgtttc gccaggatcg gccaccatat caccagtgac tgaagacatc
    18961 atcacaatgc gaccatcttt gcgcgcaatc atctccggca gcaccgcctt cgtgacgttc
    19021 catacgcctt taatattgat atcaatatgg aaatcgcgat cttcatcgct catatcgagg
    19081 aaactgccca gacgacaaac gcctgcgtta ttcaccagga tatcaatgcg cccttctttt
    19141 tccttcgcgc gtttgatagc tgcggctacc gacgccgggt cacgcacatc ggcgacaacc
    19201 gccgtacagc gatgaccacg accacacagt tcgtccgcta gcttttcgat ctcaggggag
    19261 atatccagca agattaggtt cgcgccatga cgtgcaaaag ttctggcaat tccttcgcca
    19321 attccctgca atgcgcccgt aatcagtgct gtcttgcccg tgagtttacc cattttaatg
    19381 ctctccttgt taatacagag cgttacactc cattccctct gaaaatacag gataaacaaa
    19441 atattgctgc ggatcagctc actaattcag gaaaatctca atccagtcgc tgctttatgc
    19501 atcatttcag gcgttgtgtt agctcactgc tgcgctgaat cattttcaat gacgccacat
    19561 cattgatgtt gcacaagacc aacaaacagt aaatcggtaa cagaattttg cgccgtgcgg
    19621 gtagacatcg acgagctacg ccattcggtt tcaccggaaa cggtatcgag cgtgaaatgc
    19681 gccagccgcc gcagcggaga gtcagccaga gaagtaatgg caatcaccgt tgccccctgc
    19741 tttcgtgccg cttctgcgca caagacaatc tcttttttac tgccgctgta agagatagcg
    19801 atttgtacat cgcctttttt caacgcctgg gaaacggtgg cttgtacgtg cgtgtcggct
    19861 tcgcaggcca cgcgatagcc aattttcatc agtttgaacg acagatcgcg ccccaccagc
    19921 gccgatccgc ccaggcccgt tatctggata aacggtgctt tactgatgac ctcaatgatt
    19981 ttttgcagcc gcgcgtagtc gaacaacgca caggtttgct ccagcgccag ctctttttcg
    20041 cgattcagtt tgcgggcaat cacttccagc gaatcatcac tggtaatcga actgtgcagg
    20101 tgcagcgccg tggcattcgt tttctcccgg ctggcactgt attcgccaat taacgccata
    20161 cgcaattcag taaatccttg cgcgccgagc ttctgggcaa atttcacaat gctcgactgg
    20221 ctgataccga gctgtttcgc catctgccgt gaagagacgg atttcagctc gctcactctg
    20281 gcccgcagaa agtcagcaat tttttgctcg ttcccggtaa attcactttc cgcattgcgt
    20341 attttcgtca ggtacaacat cttccactct cctgggctgg cagcaaaaat tccgcttaag
    20401 ttcttatgac gctcttcaca ctctgcgagt gtagactcaa tgattccttt aagtctgtca
    20461 attcgggata tatgattcca tacatctgca attatttgat cgtaaatagt aaggtcacca
    20521 ccgatgcaac tagaaaagat gattactgaa ggctcgaaca ccgcctcggc tgaaattgac
    20581 cgcgtatcga cgctggaaat gtgccggatt atcaacgatg aagataaaac cgtaccgcct
    20641 gccgttgatc gcgtcctgcc ggatatcgcc gcggcgatcg atgttatcca cgcccaggtc
    20701 agcggcggcg gtcgtctgat ttatctcggt gcgggaacat ccggtcgtct ggggattctg
    20761 gatgccagcg aatgtccgcc cacctacggc gtgaaaccgg gtctggtggt tggtttgatt
    20821 gctggaggcg aatatgccat tcagcacgcg gtggaaggag ctgaagatag ccgggaaggt
    20881 ggtgttaacg atctgaaaaa tattaattta acggcacagg atgtggtggt tggcattgct
    20941 gccagcggtc gcacgccgta tgtgattgcc ggactggaat acgcacgcca gctcggctgc
    21001 cgcacagtgg gaatttcctg taatccgggg agcgccgttt caaccaccgc tgagtttgcc
    21061 attacaccga ttgtaggtgc cgaagttgtt accggttctt cgcggatgaa agcaggtaca
    21121 gcgcagaaac tggtgctcaa tatgctttcc accgggctga tgattaaatc cggcaaagtg
    21181 ttcggcaacc tgatggtcga tgtggtcgcc accaacgaaa aactgcatgt gcgccaggtc
    21241 aatatcgtta aaaacgccac cggatgtaat gccgaacaag cggaagcggc gttaattgcc
    21301 tgcgagcgca actgtaaaac ggccattgtg atggtgctga aaaatctcga tgccgcagaa
    21361 gctaaaaaac gcctggatca acacggcggc tttattcgtc aggttttaga caaggaataa
    21421 cccatggcca aagagatcag cagtgaactt ctgaacacca ttcttacccg tgtcggcgga
    21481 ccgggaaata tcgccagttg tggtaactgt atgacgcgcc tgcgtctggg tgtacatgac
    21541 agttcactgg ttgatcccaa tatcaaaacg ctggaaggcg tgaagggcgt cattttgacc
    21601 agcgaccagg tgcaggtcgt ttttgggcct ggtaaagcgc atcgtgccgc aaaagcgatg
    21661 agcgagctgc tgggcgatgc accggtacag gatgccgcag aaatcgccgc ccagaacaaa
    21721 cgtcagttaa aagccaaaca aacctccggc gtgcaacaat ttctcgccaa attcgccacc
    21781 atcttcacgc cgctgatccc cggttttatt gccgccggtc tgctgctggg gatagcgacg
    21841 ttaattgcca cggtgatgca cgttccggca gatgctcagg gcacgctacc cgacgcgctg
    21901 aattttatga aggtgttcag caaaggtttg ttcaccttcc tagtgattct ggtgggctat
    21961 aacgccgccc aggcatttgg cggcacgggc gtcaatggcg caattattgc cgcactcttt
    22021 ttgctcggtt ataaccccgc tgcaaccacc ggttactacg ccggttttca cgatttcttt
    22081 ggtctgccca tcgatccgcg cggcaatatt atcggcgtgc tgattgccgc gtgggcctgc
    22141 gcccgcattg aaggcatggt gcgccgcttt atgccggacg atctcgacat gctgctgacc
    22201 tcgttaatca ccctgctgat caccgctacg cttgcgtacc tgatcattat gccgctgggt
    22261 ggctggttat tcgaaggtat gtcatggctg tttatgcacc tgaacagtaa tccgttcggt
    22321 tgtgcggttt tagccgggct gttcctgatc gccgtggtat ttggcgtgca tcagggcttt
    22381 attcctgttt acctcgcgtt aatggacagc cagggattca acagcttatt tcccatcctt
    22441 tcaatggcag gcgcgggcca ggtgggcgcg gcgctggcac tctactggcg ggcgcaaccg
    22501 cacagtgcgc tacgcagtca ggtacgcggg gcgattattc ccggcctgct gggcgttggt
    22561 gaaccgctga tttacggtgt caccctgccc cgcatgaagc cgtttgttac cgcctgttta
    22621 ggcggcgcgg cgggcggttt gtttatcgga ttgatagcct ggtggggtct gccgatgggc
    22681 ttaaacagcg cctttgggcc gtctggtctg gtggcgctgc cgctgatgac ttccgcacaa
    22741 ggcatccttc cggcaatggc ggtttatgct ggcgggattc tggtggcatg ggtttgcggg
    22801 tttattttca ccacgctctt tggctgccgt aacgtcaatc tggactgata ttatgaaacg
    22861 gacaatgctc tatctttctc tgctggctgt tagttgtagc gtcagcgccg caaaataccc
    22921 tgttctgaca gaaggtagtt gatcaagtat atgcggcgtt aaagcagtaa taaaaaggcc
    22981 agtcagtaat gttactgcct ggcctttaat caatgaatca gaaacgctta cagcgccatc
    23041 aacttgtcca gcgacggtgc gaaataatag ccgccggtta ccggtttggt gaaacgcaac
    23101 atcgcatcac gtttgccatc ggtatcgcca aacatgctca gcagttgctg ctcaatgtta
    23161 tgcagacgcg cgcagtaggc gcagaagtac agaccgtgag tgccactggc ggtgccgtac
    23221 ggcaggctct ggcgaacaat cttcagccct ttgccatctt ctttcagatc aacgcgggtg
    23281 aggtgagagg tttccggacg ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc
    23341 ccgatcatca tctcctgatc gtgaacgctc atccggttga gctgcttcag gttgtgctcc
    23401 caacgctgca caaacacata gctgccgccg gaatccacgc cgtctttgat aaccgccact
    23461 tcgcggcgcg tctcttcacc cgccgggttt tctgtaccgt caacaaagcc gctcaggtca
    23521 cgctcttcca cccaacggaa gccgtggatc tcttctttca cttcaatgca gtcaccaaag
    23581 gcttccatcg ccgcctgagc gacagagaag tttacgtcgt gacgcagaga aagaatgtgg
    23641 atcaacacat cgaactgggt cgtcggcgca aggcctttac cgtagcccgg aaaatctttc
    23701 agctcttctg ccccaacgcc gccgctcaga gcgcgccagg tgttgttacc aaaggcaacc
    23761 accgcaccaa gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat
    23821 gttttactgg ccgcacgcag ggcgtcaact tcccctttca cgttggcttc gatccaaatc
    23881 gccgcgcggc aatgttctgg caaaatgcca ctctgaacct gagacattgt tcctcctgaa
    23941 aataagaatg ccacgcaatc cgtggcattg ttgagcgcta ttttacgtgt tttttcgcga
    24001 cggcggtttg ttcagacgca aattaacgcc gccagataat tttactgact ttccagtttt
    24061 tcagggtatc gtccgaaggc attaaccctt caggaccacg ccattccccg ctgaactggt
    24121 agctaatatg ttgactgcct tcggctttac attcgacggc acgattatca tcaccatcag
    24181 ctttttggca attgccaaat gctttgctgt aaaggtcgct aaacggtgta ccgattttaa
    24241 caccggtgtc agcaggaata tcgctatcca gcacgtcaat gcgactgatc gtgccttgat
    24301 cgccgttaat caccatcgcc acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt
    24361 tgccgttcgt ggttttcatt ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa
    24421 tggcttgttc ttgcagtggt gtggacgccg ttaattcacc cacgccctgc tcgctcactt
    24481 tggtggacga tccaaaccag ttccacgggt tagcggcaga ccagttaact gacgacatcg
    24541 tggaacagcc ggtcagcatc aacggcatag cgcataacat taaacgcagc gatttcatgt
    24601 cacttccttt ggttattcaa taacgttgct tggagtgcaa attcaccaaa aagtgccgtt
    24661 attctttttc ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc cagattaacg
    24721 ccacaatatc cgccactacc agcgccagac cgataccgct aacggattca ccgttcagcc
    24781 acagccacgg ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga aaatagagca
    24841 catgccaggt gcgaggaaac gaagcccgcc gaccgctcaa caaaaacgcc agcaccgccg
    24901 gaataccggg aatcagcccc agccagaaat tatcgtgatc gggataaaac agatttagca
    24961 gcgcagtacc ctgctcacgc gacgcaccgg caatgacaaa cagcacccag gttcgcgcct
    25021 gaagcaatag cacaagccag aagagcaagg gtaaacgcag gcgtccgtgc gcatcataat
    25081 ggacaggatg gaactcagta ctcttcatct tcaatcaaac gcttacccag actcagcacg
    25141 tcggcgtgtt catatcccag gcgttcatac attccgagca ccatgtcgtt atcttccggc
    25201 acgttgatct gaattttcgg gcagccgcga gcaatcagct ttttctccag ccggttaagc
    25261 aacgcattgg caattccacg cccacgaaac tctggatgca cgccaagata ataagcagac
    25321 ccgcgatgcc cgtcataacc gcccatcacc gttccgacca cttcaccgtt tacctcagcg
    25381 accaaaaaca aactgacgtc atggttcatc ttacgctcga tgtccatttc cggatcgttc
    25441 cacggacgca gcaagtcgca acgctcccaa agggtgatga cctcttcgaa atcttcctgg
    25501 cgaaatacgc gtatctccat ggtattcgtt acctttttgc gggttaaaag gctgattatg
    25561 gcgtgaacgg tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg
    25621 ggagttgtca actattgaaa tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg
    25681 gattacgcga tacgatataa catctggaac tttattatta caactcaggc cgtatgagca
    25741 cttttaaacc actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc ggtttagctg
    25801 ccctgacgtt gtcaggaatg tcgcaagcca ttgccaaaga cgaaccttta aaaaccagca
    25861 acggacacag caagccgaaa gccaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc
    25921 caggccacgg cggaattgat accggagcga tcggacgcaa cggttcgaaa gaaaaacatg
    25981 tggtgctggc gattgctaaa aacgtccgtt ccattttgcg taatcatggg attgatgcgc
    26041 gtttaacgcg ttctggcgat acgtttatcc cactttacga tcgcgttgaa atcgcccata
    26101 aacatggcgc agatctgttt atgtcaattc atgccgatgg ctttaccaac ccgaaagctg
    26161 ccggtgcttc ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc
    26221 tgtctgaacg cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact gacaaggatc
    26281 acctattgca gcaagtactg tttgatctgg tgcaaaccga tactattaaa aacagcctga
    26341 cgcttggctc gcatattctg aaaaagatta agccggtgca taaactgcac agccgcaaca
    26401 ccgaacaagc cgcgtttgtg gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa
    26461 cctcgtttat caccaacccg gaagaagagc ggctgttagg cacggcggcg tttcgtcaga
    26521 aaatcgccac agcgattgct gaaggcgtga tcagttattt ccactggttc gacaaccaga
    26581 aagcacattc gaaaaagcga taagttatga aacccgacgc acaccaggtt aaacagtttc
    26641 tgctcaacct tcaggatacg atttgtcagc agctgaccgc cgtcgatggc gcagaatttg
    26701 tcgaagatag ttggcagcgc gaagctggcg gcggcgggcg cagtcgggtg ttgcgtaatg
    26761 gtggtgtttt cgaacaggcg ggcgtcaact tttctcacgt ccacggtgag gcgatgcctg
    26821 cttccgccac cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt
    26881 cactggtagt gcatccgcat aacccgtatg ttcccaccag ccacgcgaat gtgcgttttt
    26941 ttattgccga aaaaccgggt gcagagcccg tttggtggtt tggcggcggc ttcgatttaa
    27001 cccctttcta tggttttgaa gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt
    27061 gccagccatt tggtgaagac gtttatcccc gttacaaaaa gtggtgcgac gattacttct
    27121 acctcaaaca tcgcaacgaa cagcgcggca ttggcgggct gttctttgat gatctgaaca
    27181 cgccagattt cgaccactgt tttgccttta tgcaggcggt aggcaaaggc tacaccgacg
    27241 cttatttacc aattgtagag cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt
    27301 ttcagctcta ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat cgcggcacgc
    27361 tgtttggcct gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg
    27421 tacgctggga gtatgattat cagccaaaag atggcagccc agaagcggcg ttaagtgagt
    27481 ttattaaggt cagggattgg gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca
    27541 acgtcaacga cggcttctcg gcaaacagct gctggtaatc cgtggcaaat tgccccagat
    27601 gccagaatcc ccactgcatg gcggcgtctt ttaccgttgt gctttgcgac cacgggctta
    27661 tcagttcgcg gcgtacgacg ttcaggcgaa tgcgtttcag ccacgcgttt gggccaatgc
    27721 ctaaaatagc gtgaaacgcg ttttgtagcg tgcggcgact gacatgcagt tgattacaca
    27781 aatccagcac cgtcaccggt tcggacatgt tttccagcac atattcacgg gcgcgggaaa
    27841 gcaaccgacg gtaactctga tgactgatgc tttccgccgt caccattggt tgcgcttctt
    27901 ccagcatcgc ccccatcgcc attagcaaat tatcccccag cacttttcgc actgcaggct
    27961 gatggagatt ttccgggttc tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca
    28021 gcgcggcttt atgctgctct ttcacttcca gcgccgactg gctacgcagc atatgtaata
    28081 cccgatccgg attgtgcaaa aagttagcct gacaggtgat gacatcttca gacagcacta
    28141 cgcccaggat cgtataatcg tccggcgtac ttaattcaaa ctcggttcca ccggggcgcg
    28201 tcgctatttc tgcgcttccc agacattgcg aaccgataaa tccctgctca ccgcgcgtcg
    28261 ccggaatgcc aaaccagaac gagttcggcc agaccaggca cgactgacgc agcgccagac
    28321 cggtgtattc acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa
    28381 acttgcccgg atgcagctga tcgtaaatct gctgctaggc ggtaatcgtc aaagcatgtt
    28441 catagacatc cgttgtctgt cgttgatgaa cattatccac ttcgaccttc ggcgtgagct
    28501 tcaggttttc gggtaagggt tcatgataaa gatggtgcaa attggctgta cgggtctttt
    28561 tcatgatgtt aatgccgggt gttgtaggac acccggcacc tccgacaggt taatggggct
    28621 tgagacgata acgactactg cgtttacgta acgtcccggc agaaaagagc tgttcgagcg
    28681 catttctggc tttttccagc ggccagccaa agtgggcggc cacttctcct gccgtcattc
    28741 cctgacatac tgatgttaac agcgccaaca actcgtcagc agattccgcg acgataactg
    28801 gcgcgtcggg ttcctttacg ggttgcttcg gctggcggtt aaagccagta accagccact
    28861 gggtgtcatc gtctggccga ccgatctcct tacggctgat gacacgcccg gtacgcatcg
    28921 cggcagcaca acctgcgtcc agcgccgcac gacacgccgc cagatcgcct tccaccacca
    28981 gcgttaagcg accagggtca agcacttcgt gactgagcag acgaacgtta gctgctttga
    29041 gcatggcatc cgctgcatct atcgcggcga ccatgccgtc cacttccagc aatcccagtg
    29101 cattgatcat cggcaacctc cgttacgcac gctggattgg attacgcgcg atttccagca
    29161 ctgcatcggt aaaggcgtta caggctgctt tacacgcggc ctggctaccg gttaaaaatg
    29221 ctgccgagta gttggtttca gacggtggcg ggacgtaggt caccagctgt acgtcggcag
    29281 atttcaacgc ggcatcaatg ccgtaggtcg cttccagcgg cggtgccacc agatacgcca
    29341 tcggatcgcc aagcgtgatc ccggcggttg atgaaagata agaaccggta cgcgaaacca
    29401 catgcgccag gaaggcggtg ttttctgcgt cattggccca ctggaaagct gcgccatttt
    29461 caatatgtgc aaccatcgca tccagaccgg cacgcacttc cgccgggttt ggcccaccaa
    29521 gcataatcag cacttcaccg gcagtcggtg acgggccgtg tgccgctccc gcatacagcg
    29581 agcgaccata taccacttcg accatcgctt gtttggtcgc ttcgtcagcc gcaatgtagg
    29641 ttacgtcgtc agaatcggca gaaatcagcc cgaggctacg aatatgcggc ggcaatttta
    29701 gttcacgcgc aaattcagcg ttaacggagg caatcacccg catggcggtt accgacggtc
    29761 gaatcaaatc taaagctggc atgatgcctc cttatcgggt catgttgatg ccggatgctt
    29821 tctgctccag catacgtttc gccaaatcca caatgacggc acagcagcac gaaagagaag
    29881 caaaagtcag ctacctggta cactaggcag cgggtcagca aaggggggca aatgcccctt
    29941 ttttcatgtc agataatcag atcattacct caataaacta ataatcacat cgcggttaca
    30001 accagtttga tagtgatagc tcaaacccag acgtggcgct ggctgcaagc aaaataagcc
    30061 gcaaaaatgc gaccccatgt ttactagccg caaaaatgcg gtcctatttg ccagaacctt
    30121 ttattattca attggttagt gcaagaaaaa gcgtgattac tgttcaagaa gtatccagaa
    30181 aggtcatgat tttgacgctg aaaaaaaata ggtgggcatt tttgcccact cactagccgc
    30241 aaatttgccc actcactagc cgcatttttg cggcccaaag taataaatac agagaaataa
    30301 taaatacaga tcagcgccaa gatttttcgt cttttcggcg gaatcatgag actagtgctt
    30361 acgtcctgat aacctccaga gcagacctta acttctgtgc agcaggacta cgctcattgc
    30421 cgctcggcta ggtcaaatcc tcacgataaa gctgttcgga ttgtgaccat aatgttaaag
    30481 gactctttaa atcattcctt gacgaattaa ctagctggct taagcggaat agcatgcagg
    30541 ataaaaacaa aaccagctta gcagtgggga gagcttcgct tcgctcgtct cctggggcca
    30601 atgctcaatg aaacgacgta aggcacggag cgctcaccat aggtagacac ataaacatct
    30661 gagagcgcac cttctatcgc gctacgcgct caataaaaac caacgacacc gccacactgc
    30721 aagaccaatg gtttttatcg cgtatacaga ccagacagga aacagcagca aagcagcgta
    30781 tagccaggtg cagacagcac actcacacga cgcacagcag gcttaagtga tcactcagga
    30841 aactttatcc ccgccgccga tcaccgcgaa ctatcatgac cgcgccaaat tcgaaaggaa
    30901 tggcccatag aacgcgatga acacacataa gcgctacact tacttaacgt cgccacagag
    30961 cgattgagag cgcttaaaat cgatgcgatt tcattaaatt tttctcactc gcctgaatgg
    31021 tggcgatcag gactgatcgc aacattgccc cgcctcgata ttgagcgcat aatagataca
    31081 tacattaagc ggaaaagaga taagtttatt gtgaaaatca gggggttaca tatcatgcgc
    31141 atatcagaac aacaacggca gatcctcgac tatctggcaa aaaacgaatc aatcaccgca
    31201 cgacaagccg cagatctcgt ttatggtggc aatgttaccc gcgtccaggt tgacgccgcc
    31261 cgccgctcac tcatgacgat ggttagtaat gggctaatca ggaaacaagg ccgcgctttc
    31321 gtagctgaaa gacatatcaa tcacgcttat agccaggacg ttgaagaatt gcgccgccag
    31381 atcctttggg tattcagcaa acagggtcag gagtatttcc tttctgaaca cccaaatttc
    31441 gccgccaaag agttaacagt aagcggattg tcgtgcgcac tggattatct tgtcggtgaa
    31501 ggttgcgtta cttacaagca gctatggcac gccctgaata cgcttgtaga agatggttta
    31561 ctcattgcca cgcgtgagaa catcgaatac tggataatgc cgcgttcagg tcgccgcaat
    31621 catgcacggt atatttcagg caacagatac agaacgccgg actttaatcc gacacaggaa
    31681 gatcgggacc gtgccgaaat gatgtgcagg gaacaggaga aaagaattat ggataagtta
    31741 ttttcgatgc agtgaacaca agcgggcgca atgcccgttt ttattgcctt attccacgcc
    31801 tgacgaatca atttgatcac acctgagaaa atgcatagcc agaagttaag gccgttatat
    31861 tgcgattggg agcgttttaa acggtatcaa tttaattaat tctgatgtgg tgatttgcag
    31921 gcaaaaaaaa cccgccggag aagcgggaaa tagagacata tcgttgtagt tattaacaga
    31981 tcactttgag gtcgagggcg gctaaccctc gctaatatta tcccgctatt accttgcggg
    32041 tgcaaataat cttttattgc tttgatgatt taataaatag cactttttgc taacaaaaac
    32101 cgctgtcaat atatggccaa aaatgtcaac agaaaaaata aggtttataa tattaagtat
    32161 cacatgggta atgggttcag ggaatcggaa tccaccgcct cacggaaatt caaaatttgc
    32221 ttcttctttc ctccgtgggc ggcttctttt tcgtatcgag gtatttatga aagcaataac
    32281 attagaacag ttcgcagcaa tggcgaatgt atcgaccaga acgctgaggc gctggctaaa
    32341 aaaaccatca accggagtcc ctttgccatt taccccacgc ggtactcgcc aattattctg
    32401 ggagcacgaa tgcaaagaat ggttgtacag aacagaacga taataatcac gcatcacatt
    32461 ttgaggtaaa catcatgacc aatatcgaaa gattagaacg ctgtgccgat attatgcgac
    32521 gcagatggat ttatgaccct gaagcgggtt tattgtttag ccgtgaaaca aaagacgtta
    32581 tcaaaggcag catgaccaat aaagggcatt tggttgtaac ggtacatgaa ggtaaattca
    32641 aagtgacact cacctatcag aaagcctgct atgtatacac atacggcgct tatgacgaga
    32701 cattatatga agtgcaccac gttaatttga acaaacagga taaccgcatc aagaatattc
    32761 gcctgatgcc gaaagaaaag caccgccgga tacataaaaa cgtccgcaaa cttattcggc
    32821 tgggccacac acaatggctt caaccgcaat taattgaaca atcactataa acacaggagt
    32881 aaataccatg cattcattag acagttattt tcagcgcacg acagccccta aatcagccgc
    32941 ccaggaacac cgtgaggaat tccatgagaa agtgatgcgt tctgctgatt atattgcgga
    33001 caagttcgtg gaaacggttc gtccacttgt tgatgaagta gctgataaac ttcagtctga
    33061 aatgccggaa gatatggaag gcactgcaaa gcggcgactc atttgcgaat taagtcgtcg
    33121 ttttggcgtg tcaataagcg cttttaaata atcgaaaaaa ggggggaaag ataatggacg
    33181 catttgaggt actggcaaat aaaggcgctg aactggtagc acagcgtgat aaaaccgcca
    33241 acgaagggga acgcgcactg ctaaataaac agattaaagc gatccgtatg gcccagttta
    33301 aattaatcag taatgaagtt atcgaaacaa ttaaaccgca gatcccacaa atcgttgctg
    33361 acgctattaa agccgccgga ctggagaaac gcattgccgg gctaaaaacg aacgcagaga
    33421 aaatggagat gtacaaacag agcggtgctc cgaatcccga tgagtacttc tcaccggatg
    33481 ttgagtttat ggcgcaggtt gaggaacgtc tgggtagctg tctcaccgag gaacagcgtc
    33541 gctattttga tggagtggat agttcggcag gtattgacct gaactcgtat ttcggtcgtg
    33601 aaattgagca ttttgatgcc gccccgtcta tgccggagcc agaaccagaa atggccaccg
    33661 atcctgaagc tgagcaacgg aaacgcgtat tcaatgcgat agggattcgg tactgatcgg
    33721 ataaaagaaa gccgggaatt accccggctg acttaacaat gtgaccaatg aacaaatcgg
    33781 acaagacaaa caggataagc agtctcgaaa caatgttcgg gaactatatt agcacaagtc
    33841 cggagaaaat catcatgttg gcactacgca tcatgcaggg catcgctaag accctcgcag
    33901 aacatgtctt agacctgaaa cattcgccac tgagcaaaca ggccatgaag cgccaaacgc
    33961 ttcgtctgtg ggctgaatat tcgcttggaa ctatcaataa aatcatcgac atgaaatcgg
    34021 ggccgagcaa ccagagcgcg gaggaaatgg agttcatcag gcgtttaatt ctcattcgca
    34081 gagacattca tagccagtta cacagcgtgg gaatcgatat taatgatggg acgggcgatt
    34141 aattttcgga caagtactat gcgcgcgcga ggagctgaac atgaagcaag atattgcaga
    34201 caggctggag atactcgaag gccagcgggc agaagcgaaa caactacgta agcaggccag
    34261 aagagcgcac aggaacaacg aagcagagtt actaaccaaa tacatcagtt tcaccaatta
    34321 ctgcatctac gagtgttaca aggaagatgc agaggactgg ctggattcat tgccagaaca
    34381 gtactgagca gaataacacc acgtcatgtt gttctgaggt gagcatatca cctctgttca
    34441 ggtggccaaa tttagtgtgc cacttcagca tcaccgttga aatcacaata acgatttttt
    34501 tgtaaatatg ctctcacata tgtaggaata gaccaggttg gcattgaaac acatctacca
    34561 tgaccataat aaagtaattt tttgatatgt ttatggtttt atattaatta aaatatatct
    34621 aaatttgata gttttaaaat tgcaaaatcc agattgtgtt tgtaatgttt aatctgattg
    34681 tattgagtgg cgtttattat gaaaaataaa tcaggtaatt cttgttatag agaagcagtc
    34741 gatattcata gctcacttga gctttcattt atattgcata aatatcattt gcgtgtagat
    34801 gagaagaaat ctcgaaatgt attcagcgcc atacgtgatg agctcatcct tgatggggat
    34861 tcacgacaaa acttagctac tttctgtcaa acatggcttg atgatgaaat ccgtgagttg
    34921 cttaatttag ctattgataa aaacatgatt tataaagatg aatatccaca aatggcggaa
    34981 cctgaaggtc gctgtgtgcg tatgctttcc gattcgtgga gtttccccga ttcgcgacac
    35041 accttaggtt gctcaaccat tggttcatct gaagctgcta tgctcggccg cttggcgtta
    35101 aaatggcagt ggtgtaaaaa acgcgaagta caaggtaaat caaccgaacc tgatttgcga
    35161 tcctgtgcaa atatgctggc ataaattctg ccgttactgt gatgtgaaat tgtgtgaaat
    35221 tccacttgat gatcatcacc tgataatgaa tgctaaagag gttttgaagc atgtcgatga
    35281 aaacactatt ggtgtcgtta cgactttagg tattacttat ttttgtaaag cagattgcgt
    35341 tagtgttttt aagataagtg ttaaagttaa tattagtttt tatatttaca tcgtaattat
    35401 atgtttctat tttaaaaatg ttcgagagta taaaaatgtc taatgctaat atcgcaccca
    35461 aaagtaagaa aatgacagtg gggacactcg ctatcatgaa tataactgct gtagtaagtc
    35521 tacgatttat accttctgaa gcagaatacg gcttaggtgc cattttttac tatacctttg
    35581 ctgcaattat gtttttagta cctgttgcct ttgttgcggc tgagctagct acaacatatc
    35641 cacaaaaagg gggggcattt cgatggatag gagaagcatt tggggctcgc tggggttttg
    35701 taggaatgct tatgacctgg ctacaagtca tcccctactt tccaacagta ctgacatttg
    35761 gtgcagtctc ggttgcattt attgatgttg atatgggggt ggcagaatca attgccagta
    35821 ataaatggta tattttattt ttcgtactat ttgtttactg gggagctatt tgtgttgcat
    35881 tacgtggcgt tggtattttc tcgcaggtat ctaagtggtg cggtattata gggacaatta
    35941 ttccagctat tatccttgtt attcttggtt tctcctatct gtggttcagc ggtaaaccag
    36001 cggtgatccc attaggttgg ggtgatcttt tccctaatat agctaacttc cagaatattg
    36061 ttcttgccgc cagcatattt cttgcttatg cagggatgga aatgaatgcg gtccatgtta
    36121 acgatttaga taatccaact aaaaaatacc caattgcaat taccatcgct tctataggta
    36181 ctattgcaat attcctgctc agtactttag gggtcgcctt tattattcct acagataaaa
    36241 ttagtctaac tcaaagtttg ttggttgcct acgatatgct gttcgaatgg gctggagtgc
    36301 catggcttgg ctctgtgatg gcatttatgc tagcaattgg cgtactgggt ggggtggtta
    36361 cttggattgc agggcctaat acaggtgtat tagcgatcgc gaaagctggt tatttaccta
    36421 agtttttcca aaaaaccaat cgtcatggaa tggggcacca tctaatgttt gtacaaggta
    36481 taattgtctc agttctttct gttacttttg tgataatgcc ttcagttcaa gcagcttttc
    36541 agattttatc acagttaaca gttatgctct atttagttat gtatatgctc atgttcgcta
    36601 gtgctatcta tctacgacat tctcaacctg atacaaaacg cccatatcga gcccctgctt
    36661 tatttttctg ggcaacactt ggattcttag ggtcttttct ggcttttgta ttaagtttta
    36721 ttccgcccga gcaaatccca actggtagtc caaccagcta tgttctttat ttgttagtat
    36781 tagttgccct atttttatta attccaatat taatttacgc cttccgaaaa tctgactgga
    36841 aagatccaaa ctctgatttc gaaccatttt cttgggaaaa aagaactagt aattaaattt
    36901 tccataagct acaaaagatg aggttaaata tgtttagtgc tgaatctcca attggtgtaa
    36961 tagactcagg tgtcggtgga ttgacatcgg taaaggaaat aattaatttg cttcctggtg
    37021 aagatattat ctattgtgga gataatatga atgcacctta tggcaaccgt tctgccgatg
    37081 atatcatcag tttgactaaa aaaatgctga cttttttaca gtcaaggaat gtcaaactag
    37141 ttgctgtcgc atgtaatact atatcctcaa ctttggaatc cgaagaatac agcggatatg
    37201 ctaagagctt tccgtttcct atcttatcca tcattgaacc tgcagtagaa gatgtgattc
    37261 gccagcaata taaaaatgtt ggtattatcg ctaccgagtt tactattaaa acggggtgtc
    37321 acaaagagct aataaaaaaa ttgaattcaa caataaatgt atttggagaa ccaagcaaaa
    37381 atcttgcaat gttaattgag gaaggtaatc ttaatgctcc tgcaatcttg aatgacatca
    37441 aaaaacatgt taaccattta atttccctcc atccagtaaa tgaaattatt ctcggctgta
    37501 ctcactatcc aattgttcag aatttttttg aagcagttgc accggatata aaatttatta
    37561 atcctgcaca tgatcaggct atctctatta aaaatcactt gggacagcta aatttattaa
    37621 actcctcaaa tataggaact ttgcaaataa atactagtgg aagtatggag atctacaaaa
    37681 cggtactagg ggaattgagc attacgaaac ctcatacatt cagtatcagg cagttttaaa
    37741 tttatccggt acaccgtaaa atcccgtcct tctgaactgc accccaaaag ttggacacca
    37801 actgagtaag gtgcatttta tggacaaacc cagttacata ctgggtagtg catccaatta
    37861 gtagaacatg tgtttttcga taaacgctcc gatcactttt tcgtggatct caactgagcg
    37921 tgagaagcag attgttttac gacccaaccg cttaatgcgg gtgcgtagcg tcagattatt
    37981 acgctcaatg cgttgggtga atattttgcc ggtcagatgc ttattcttcg gcacctcccg
    38041 gccatagctg ccccagtcat cgctggtcag catgccgatg ttgaagggtg taagcagtgc
    38101 cagtagctcc cggcacgttt gatcggttcg gggaccaaaa gtgtaggcca gtacaccgcc
    38161 tgttttggtg ttgtacgcgt acccgagcca atgttgccgg gctttactgc caacgtagct
    38221 ccattgctca tcaagctcgc agataaacgc cacatcagca tgggcaacag gcgaagaagt
    38281 tattcgcttt ggcgcgagtt ttttaaagtc cggatgacgg tgttactacc aattttcagt
    38341 gtcctggcgg tatcgcgaac cccggcacca ttaaaggcca tttcagttat cagctctttc
    38401 atacccggct tacgtgcttg ataagtataa gtgaactgaa acacacggtg gcagtcacgg
    38461 cagcgaagtc tgtcacggcc tttagggttc tgaccatggc ggtaaacctg agctgactga
    38521 caacggggac aatgaatatt aacgctggtc atgagataac ctcaaaagcc cgtattatac
    38581 atcagattca actaattaga ggcatcaccc atccagcatt ataaaacata ctaacaataa
    38641 ctaaattata ataatcacaa cttacaatca aataataaat gcataagatt aacaatgatt
    38701 ttttcgggtt atatttatct aaaaaagcag aacattatgt aacttattat tttctaagcg
    38761 aaaatttatt gtgcggtcag actaacaaac ttgcgtacca cccgaagaac atgcgaagat
    38821 ttaagtattc tttctttttt ggtttttatt tttcattttc tcttttcttc tctttccggt
    38881 tctgttctgg ttttggtttt ttccttttta tttttattct ttcttcaggg ttatgctaaa
    38941 acaccaaaac atttttcacc acaaaagcat ccaatcatct tgccagacca atcaccttaa
    39001 ccggatacac tgccggatat aatcccacta acacccagac accaagcacc atacctgata
    39061 actaccgtat tcaggaagga aatgcgagtg atttcaccgt caccggcaca accgatccgc
    39121 caaaaagagg cgtaccaatg tcgatatagt cccccgcgcg gacaatcact tcgtcaatga
    39181 ctgccaacgg gagttgttgt agctgtgggc gcaacaacat gcccagagct ttaccaaagt
    39241 cctgcccggc caccaccagc aggggatgcg gattcggaaa acgcgcgacg aaatcgacca
    39301 gcgcgttgat gaccgtcaga accgcggcgt aacgcacagg cagcgaggcg ggaagcgcca
    39361 gcacgtacac gtcagttttg ggatcaagat ccagctgaag cagcgcctgt tgccaggcac
    39421 tcaccagatc cgtttcatca atcgggatcg ccaccggcaa attgcgcagc ggcagttgta
    39481 cgccctccag ccagattgtg ctgccagaga gcgaaagggt atgtgcaccc gcgccaatca
    39541 ccgtggagcg tacggtttgc gccggaaact gcacattcat ctcacgcagg cgcggatggt
    39601 catgcagcgc cgttgccaga agtgggccaa tatcggcaaa acagaacggg ttggcgggct
    39661 ggtggcgata acattcgccc acgcctccag aaagcgtaat gatttcgggc gtaacacctg
    39721 cgggcagcaa accggtttgc atcaatgcct gcgcgagcgg cgaaagcgtt ccgtcaatca
    39781 cttcgacaat cagcgccgcc atccgccggg taacctgcac cagctgcgcg ccggtcagcg
    39841 aacgggcgtc agtgcctgca ccgaagcact catccacaat catctgcccc ggtttatgag
    39901 cgtaaaccac gcgcccctgg ctgtcggttt ccagcaggcg accaccgacg ttgagacagg
    39961 cagtgccgct gatttttccg gcatcgaaca gggcgtagtt cgcggtgcca ccgccgatat
    40021 cgatattcag tacccgacac agccgttgtt cagaaagggt ttgcgccccg gctccgtgac
    40081 cggcgatcac ggattcgagg tgcggcccgg cgctggcgac gacaaaatcg cccagcgact
    40141 gtttttcctt catcactttt tccatcagct gtgcgaccag gctttcggta aactggcctt
    40201 ccggcagctg ggcaatgatg gtttcgcgga tgcgctggct ttcggttttg tcggctggca
    40261 cgctggcggg cacggacgcc gttaccggga tcgattcatc acattcggtg atggtaaaac
    40321 ccagtagatc cgccacttca cgggcttccg gggtaataat gctggcgcgc agaaccacgg
    40381 acattgcctg ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg atgagttttt
    40441 tcacctgctt cctcctgttt ggtgagagat gccagataat ccaccagttg ctgcacgctt
    40501 tgcgggtcgt ggctgtttag ctcgaaaatc ggctcttcga aacccgtctc aagcaataac
    40561 tttcgcgttg cggcgacgtc agcatctggc atgtccgttt tactgatgac ggcgatttgc
    40621 cgtttgctga cgccaatatc cagcaacccg gcaggcaacc gactttccgg gtcatttgcg
    40681 ccatggacat agatcagcat gtctacatcc tgtagcgtgg taattaaggc gtgataccag
    40741 cggggatggc taaaatattc acccggcgta tcaatatcgc ccttatcatt aaattccacg
    40801 gccagtgttt ttctggcgag ggtataattc ccctgcaacg cattaaataa cgttgttttt
    40861 cctgccccga cgctacccac aaacgcaatt cgtttcatga atgcctcgta ttaacttttg
    40921 gtcatttcac aaagcgtata atttaataag cgacctaaac cgctgacggt ttgtgataac
    40981 gcttcttcta ctgcgcccac cgagccataa atcaccaatg caccgctgaa gcggtcgaga
    41041 aaaccgatat gcacatcagc ggctttcagc gctaaatcac cggcgatcat cgccgtttcg
    41101 ccgggagtta gcgtcataat gccaattgcg cccgcatccg gaacgccgat tttcttcgcc
    41161 agttcctcac cgggatgcgc gatgagatgc gccagtgtga cctgtttgcc gggcacaaat
    41221 tcctgaatga tgcgttcttt atccatgagt cgccgcctgt gaaaaacttt cgtgtatcgt
    41281 gacaattttt cgtcagagta aataacaaaa ttcggcaagc gcgtttaaaa ggtgaggtag
    41341 atcaataaga atggagagat atttactgtt atgaataaac tttcatatgc gacgcatgaa
    41401 tataaataaa ataatatcga gatattttat atactgattt ttttattatt cgcaaactca
    41461 gtaataagct ttgctaatat atttcaggaa aaccgcttac cctgaatatt cagggtaagc
    41521 gtgagagtta agaatgaatt acagcggttg ggtttgcgct tcaaccacgg ccagcgccac
    41581 catattgacg atacgacgca ccgatgcgat cggcgttaac acgtgaaccg gtttcgccac
    41641 gcccatcagc accggaccga cagtcacacc ttccgagctg gaaacgcgca gtaagttgta
    41701 actaatgcgg gcagcttcca tgttcggcat caccagaata ttggcagaac ctttcaaagg
    41761 gctgtccggc atacggtcgt tgcgaatcgc ttccaccagc gctgcatcgc cgtgcatttc
    41821 accatcaatc atcagatctg gcgcacgatc cctgaccagt tccagcgcct gacgcatttt
    41881 gctcgacgac gggcagtcag aagaaccaaa gttggagtgc gacaacagag caacgcgcgg
    41941 ctcaatacca aaacgacgga cagtttctgc cgccatcaag gtgatctccg ccagctcttc
    42001 tgcgtctggt tcgtcattga cgtaggtatc ggcaataaag gtgttaccac tcggcagcag
    42061 cagcgcattc attgcacctg cggtgtgaac gccatcgcga taaccaaaga catttttcac
    42121 cacactaaaa tgttcatgat aatcgcccac cgtaccgcag atcattgcat cggcttcccc
    42181 acgctgaacc atgatcgcgc cgatcactgt cgggttactg atcagcgcac gctgcgcctg
    42241 ttcctgagtg acaccgcgac gcttcatgat ctggaagtat tcggtccagt actctttaaa
    42301 gcgcggatcg gattcgttat tgacgatttc aaaatcaacg cccgctttga tctgcaagcc
    42361 cagtttctga atgcgcattt cgatcacgtt cggacgaccg ataaggatcg gtttcgccag
    42421 tcccagcgtt accagttcct gagtcgcatg cagaacgcgc gcctcttccc cttccggcag
    42481 aactacgcgc ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa acaggttggt
    42541 tttgtaaacg aactcagtca gcttgtcgat gtagacgtcg aaatcagcaa tcggacgcgt
    42601 cgccacgccc gactccatcg ccgcttttgc gaccgcagga gcgatcttaa cgatcaagcg
    42661 cggatcaaac ggttttggaa tgatgtattc cggaccaaag ctcagatcct gaacgccata
    42721 cgctgaagcc accacttcac tctgttctgc atgggccagt tctgcaatcg cgcgtaccgc
    42781 cgccagtttc atctcttcgt tgatggcggt tgcgccaacg tccagcgcgc cacggaagat
    42841 gaacgggaag caaagaacgt tgttcacctg gttcgggtag tcggaacggc cggtacagat
    42901 gatggcatcc ggacgcactt ctttcgccag cggcggcaga atttccggtt ccgggttcgc
    42961 cagcgccagg atcattggtg cccgagccat tttcttcacc atttcctggg tcagcacttt
    43021 cgggccggaa cagcccagga aaatatccgc accttcaatc acatcatcga gggtacgttt
    43081 gccgtcatcc accaccgcat atgcggcttt ggtttccgcc atatttggct cacggccctg
    43141 atagataacg ccttttgaat cgcaaaccac gatgttatgt ttttgcagcc ccagcgctac
    43201 cagcaggttc atacaggcga ttgctgcggc acccgcgccg gaaaccacca tccgcacgtc
    43261 ggagatgttt ttctccacca cgcgcaagcc gttgaggatg gcggcagtgc tgatgattgc
    43321 cgtgccgtgc tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca gtttctgttc
    43381 aatatagaaa cattctggcg ctttaatgtc ttcgaggttg atgccgccga aggtcggttc
    43441 gagcgcggcg acgacttcaa taaatttgtc cgggtcgagt tcatcaactt caatgtcaaa
    43501 tacatcaatc ccagcgaatt tcttaaatag aacgcccttg ccttccatca ccggtttgcc
    43561 tgccagcgcg ccaatgttgc ctaaccccag caccgccgta ccgttagaga tcaccgcaac
    43621 caggttaccg cgcgcggtat atttgtaggc ttttagcggg tctttttcga tttcaagaca
    43681 aggtgcggca acgcctggtg agtaggccag cgccagatcg cgctgcgttg ccagaggctt
    43741 ggttggagaa acctggattt tccctggaac tggaaattca tggaaatcaa gtgcactttg
    43801 ttttaactgg tcatccattt ggttgttcct ttcacgtaac gttcacaaat aaagtgtgtg
    43861 ggcaacagcc cctgcccaca acgtggcgca cattattacc ctgccggagt ctacagactt
    43921 tgagcaagtc caaactctca ccattaatat aatgttttgg taataatcct ataacactga
    43981 tgttacctgc ttaatccagc aataccatgc ctgtctgcta tgcttttttg atgcgtttag
    44041 cgaaatttct cagaagtgtg aattaacgca ctcatctaac actttacttt tcaaggagta
    44101 tttcctatga acgagttaga cggcatcaaa cagttcacca ctgtcgtggc agacagctgc
    44161 gatattgagt ccattcgcca ttatcatccc caggatgcca ccactaatcc ttcgctgtta
    44221 ctcaaggctg ccggattatc acaatatgag catttaatag acgatgctat cgcctggggt
    44281 aaaaaaaatg gcaagaccca ggaacaacag gtggtcgcag cgtgtgacaa actggcggtc
    44341 aatttcggtg ctgaaattct caaaatcgta cccggttgcg tgtcaacaga agttgatgca
    44401 cgcctctctt ttgataaaga aaagagtatt gagaaggcgc gccatctggt ggacttgtat
    44461 cagcaacaag gcgttgagaa atcacgcatt ttgatcaaac tggcttcgac ctgggaagga
    44521 attcgcgcgg ccgaagagct ggaaaaagaa ggtattaact gcaacctgac gctgctgttt
    44581 tcttttaccc aggcgcgggc ctgtgcggaa gcaggcgttt ttctgatttc gccgtttgta
    44641 gggcgtattt atgactggta tcaggcacgc aagccgatgg acccgtatgt ggtggaagaa
    44701 gatccgggcg ttaaatcggt gcgcaatatc tacgactact ataagcaaca ccactatgaa
    44761 accattgtga tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt aaccggctgc
    44821 gatcgactga ctatcgcacc gaatttgctg aaggagctgc aggaaaaagt ttctccagtg
    44881 gtacgtaaat taatcccgcc ttctcagacg ttcccacgcc cagcacccat gagcgaagcg
    44941 gagttccgtt gggagcacaa tcaggatgcg atggcggtag aaaaactgtc tgaaggcatt
    45001 cgtctgttcg ctgttgatca acgcaaactg gaagatcttc ttgccgccaa actataaacc
    45061 agccacggag tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg cactcagtat
    45121 ggatgctgta caaaaagcca attctggtca tcccggcgcg ccgatgggca tggctgatat
    45181 tgccgaagtg ctgtggaacg attttcttaa acataatcct accgacccaa cctggtatga
    45241 tcgcgaccgc tttattcttt ccaacggtca cgcgtcgatg ctgctctaca gtttgctaca
    45301 tctgaccggt tacgacctgc cgctggaaga actgaagaac ttccgtcagt tgcattcgaa
    45361 aaccccaggc cacccggaga ttggctatac gccaggcgtt gaaaccacca ccggcccgct
    45421 tggacaaggt ttggcgaacg ccgtcgggct ggcgatagca gagcgtacac tggcggcgca
    45481 gtttaaccag ccagaccatg agatcgtcga tcacttcacc tatgtgttta tgggcgacgg
    45541 ctgcctgatg gaaggtattt cccacgaagt ctgttcgctg gcaggcacgc tgggactagg
    45601 caagctgatt ggtttttacg atcacaacgg tatttccatc gacggtgaaa cagaaggctg
    45661 gtttaccgac gatacggcaa aacgttttga agcctatcac tggcatgtga tccatgaaat
    45721 cgacggtcac gatccgcagg cggtgaagga agcgatcctt gaagcgcaaa gcgtgaaaga
    45781 taagccgtcg ctgattatct gccgtacggt gattggcttt ggttcgccga ataaagcagg
    45841 taaggaagag gcgcacggcg caccactggg ggaagaagaa gtggcgctgg cacggcaaaa
    45901 actgggctgg caccatccgc catttgagat ccctaaagag atttatcacg cctgggatgc
    45961 ccgtgaaaaa ggcgaaaaag cgcagcagag ctggaatgag aagtttgccg cctataaaaa
    46021 ggctcatccg caactggcag aagagtttcc ccgacggatg agcggtggtt taccgaagga
    46081 ctgggagaaa acgactcaga aatatatcaa tgagttacag gcaaatccgg cgaaaatcgc
    46141 tacccgtaag gcttcgcaaa atacgcttaa cgcttacggg ccgatgctgc ctgagttgct
    46201 cggcggttcg gcggatctgg ctcccagcaa cctgaccatc tggaaaggtt ctgtttcgct
    46261 gaaggaagat ccggcgggca actacattca ctacggggtg cgtgaatttg gcatgaccgc
    46321 tatcgccaac ggcatcgcgc accacggcgg ctttgtgccg tataccgcga cgttcctgat
    46381 gtttgttgaa tacgcccgta acgccgcgcg gatggcggca ctgatgaaag cgcggcagat
    46441 tatggtttat acccacgact caattggcct gggcgaagat ggtccgacgc accaggctgt
    46501 tgagcaactg gccagcctgc gcttaacgcc aaatttcagc acctggcgac cgtgcgatca
    46561 gatggaagcg gcggtgggct ggaagctggc ggttgagcgc cacaacggac cgacggcact
    46621 gatcctctca aggcagaatc tggcccaggt ggaacgtacg ccggatcagg ttaaagagat
    46681 tgctcgtggc ggttatgtgc tgaaagacag cggcggtaag ccagatatta ttctgattgc
    46741 caccggttca gagatggaaa ttaccctgca agcggcagag aaattagcag gagaaggtcg
    46801 caatgtacgc gtagtttccc tgccctcgac cgatattttc gacgcccagg atgaggaata
    46861 tcgggagtcg gtgttgcctt ctaacgttgc ggctcgcgtg gcggtggaag caggtattgc
    46921 cgattactgg tacaagtatg ttggtctgaa aggggcaatt gtcgggatga cgggttacgg
    46981 ggaatctgct ccggcggata agctgttccc gttctttggc tttaccgccg agaatattgt
    47041 ggcaaaagcg cataaggtgc tgggagtgaa aggtgcctga tggtgatcgc cggatgctgt
    47101 atgttcccct caccctaacc ctctccccag aagggcgagg ggacggtccg tgcgcttttt
    47161 agggccgcat ctgcgatttt gtttaacggg tgatccacaa cgtgggcctg gcgtctggcc
    47221 catgccagtt atcgcaggta ggttctgcgg cgtaacgcac caggcgaaaa cgctgaccgt
    47281 caaagcgcca gcgcgcctga atgccacaat agcttaatcc gcgccctttc gctaaggtca
    47341 ccagttcacg cgatttctca tcaaatgttg cgttcatcag ttccagttca ttcgtctcct
    47401 ggccgttgtt gaacggcaaa cgcaaccgaa ccgggcgcga ggctagcggc tttttacgcg
    47461 acacaatcca tgccaaatca atggtgttat aggcccctgc ctcacagcta atcatcatca
    47521 gtgctttatc atcggtcagc gcagtgacat tcacctcacg acgtaacgga tcaagcgagc
    47581 agcgcagacc attcatccgc cagtttccat aatccagcaa atcgttacgt tcttcgagtg
    47641 agagtggcgt cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc gcgggcggta
    47701 cgctgagagg cggttcgtcc cctttcttga tccacgcggt ttcactgcca acgcgctttt
    47761 gctgagcatc aataaacaac aacgccgctt ttaagccgct cagagaaatg gtctgattgc
    47821 cgtcgcgtaa ggtgatcgcc ttcccttcct gaattatctg caaaaacgcg gtgatggttg
    47881 ccgtatcatc ggttactaat aaccatggtg aaatccgcca cttgtcacca cttaatgcca
    47941 acggctcacc atctaatagc agccgtggcg ctatctcccc ttcagacgca tccggcgact
    48001 tcaacccacc gcgctcaata cgtaaaacgg catcggtatg cgccccagcg ctgcggctca
    48061 gggtcatcac cagtccatta tgatcgcccg tattgcgcgc cacacaaaaa ttttgattat
    48121 tacaggtgac ctgccagtcg gaaaacgccc gttgcgctgg tgccgcccac accaaagacg
    48181 tcggcaacaa agcgaaaaaa aagagaagga aaatgcgata gcgcatggac ggtacgaccc
    48241 cagaagttca aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg cggctcaatc
    48301 ggatttgtcg gattgagcca ataataaaca acgaaatatt attgctattt ttcagtccat
    48361 taaatgtgac atttgcaaat agttaagcaa taacaccgtc ttaccgtcac gtatctcgcc
    48421 ggttttgatc atctccagcg cctggctgaa cggcagctcg agcacttcaa tatcttcatc
    48481 ttcaacaccg ccaccggcgt tagcgcgctg attgtcactg tattcggcga taaaaaagtg
    48541 gattagctca gtcacaccgc caggcgacat atacagttca aataatttgc gcacttcgcc
    48601 cacttcatag cccgtctctt caatcgcttc tttacgaatg cacacttccg gttcgtcgtt
    48661 atccagcagc ccggcgcagg tttcaatcag ctgcccgctt tcattgccat taacccaggt
    48721 agcgacacgg aactgacgaa tcagaaccac gctctttttc ttcgcgttgt acaggaggat
    48781 cgtcgcacca ttaccgcgat cgtaaacttc acgtttatgg cggataactt cgccatcttt
    48841 gcgcgtgaga tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga gaattttgtc
    48901 tttaatgagg gtgatttgtt gcgtcatacc gactccacag cgcgaaatga acaattatat
    48961 tacgctgtga agtcgggttt gtctgcgcag gctatcagtg agatttgact gattttaccc
    49021 ccagccagtc aataatcccc tgtgccgcat gacgtccttc tgccattgcg gtaaccacca
    49081 gatccgcacc acgcacggcg tcgccaccgg cgaagatttt cggattggtg gtctggtaac
    49141 ggtactggct ttccacatcc gcgatgatgc ggccccattt gtctaccgtt acaccgtgcg
    49201 actccagcca cggcatcccg tgtggattga agccaaacgc cataatcacc gcatcggctg
    49261 gcatgacaaa ttcactgccc tcaatcggta caggtcgccg acgcccctgg gcatccggct
    49321 cgccaagacg cgtgcgcagg aaacgaattc cgcaaacgtg accttgctca ttcagctcaa
    49381 gcgccaccgg ctggacgtta aattcgaagt tggccccctc ttcgcgggcg ttcttcacct
    49441 ctttcttcga gcctggcatg ttagcttcat cacgacgata agcgcaggtg acgttactcg
    49501 cgccgtggcg cagcgcagta cgcacacagt ccatcgcggt gtcgccgccg cccagtacca
    49561 cgacgttaag tccggcggta ttgataaacg gctcttccgg tagctcttcg agccccatca
    49621 cctgtttagt gttggcaatg aggaacggca gcgcgtcata aacgcccggc gcatcttcat
    49681 tgggtaaacc cgctttcatg gaacggtaag tgcctacgcc aacgaagacc gcgtcgtatt
    49741 gttccaaaag caaatccaaa gagacatctt tacccacttc acaattgagt tcgaagtgaa
    49801 tccccatcgc gctgaagatt tcccgacggc gtgccagcag ggatttatcc agtttgaaag
    49861 aaggaatgcc gaaagtgagc aagccaccga tttctggatg gcgatcgtac accgtcaccg
    49921 ccacgccatt gcgggtcaga acatccgcac aggccagccc tgccggacct gccccgataa
    49981 tcgccacccg cttgtccact ttggtgacat gacttaagtc aggacgccaa cctttcgcca
    50041 acgcctgatc tgaaatatag cgttcaatgt tgccgatagt taccgcgccg tgctcatcgc
    50101 gaatagtaca ggcaccttca cacaaacggt cttgcggaca aacgcgtccg gtaatttccg
    50161 gcagggtgtt ggtctggtga gaaagctcga cggcggcgtc gatgtttccg gctttcacca
    50221 gttcaatcca ctgcggaata tggttatgca gcgggcaggt ccattcacaa acgctgtgct
    50281 cgccgaactt aaggcagcgc gaggcttccc gttgtgcctg gtcggcgcga aatggcagat
    50341 aaatttcatc aaaaccggtt ttgcgcgctt caatcgccag tttatccggc tcgccacgcg
    50401 cgggcgttgc ctgcatttgt tcgactttac tcattaccgg catttcttgt gcagcggtac
    50461 tggcatgcca cggttgatgc tcctgacgtg cggtgcgcaa gcggcgggat ttcgccatgc
    50521 cggagagtgc gacgtcagtg accagttgca gcgcgtccgc cgggcaattc tcgacacagg
    50581 caggaccgtt ttcgcgccct gcacaaaggt cgcatttatg cgccgtggct tttacttttc
    50641 ctgccgcgac gggtgtcagg acgatttgca tcgtaccaaa cggacaggcc accacgcagg
    50701 atttacagcc aatacacttt tgctgattga cctgaatgct gtcatcaacg tggctgattg
    50761 cgccattagg gcagctacgg gcgcagggcg catcttcaca atggtgacag gtcactgcac
    50821 tacgttgctg ctgatgtttg ataaccgtaa ttcggggatg aaaatggtgt tggctcagga
    50881 catgttgctc atcattgtga gccatgacac aggcgatttc acaagcatga caacccagac
    50941 attgctgact gttggccata ataaaacgat tcataacgac cttctttttt ggttgtaaaa
    51001 accttattct ttatatgagt gttgttatta cccgacttac aggggaaccg gcaatgttca
    51061 tgtgcccaga ataagtaact atttcgctat aaactgtggc agatcaaata atcccatcac
    51121 tgactaaatt gcgtttcagc gaactggaac attaatgatt ttttgtggag aagacgcgtg
    51181 tgattgttaa acgacccgtc tcggccagtc tggcccgggc ctttttttac attgtgctgc
    51241 tgtcgattct ttccacgggt atcgctctgc taactctggc gagcagtttg cgcgacgctg
    51301 aggctatcaa tattgccgga tcgctgcgta tgcagagtta ccgcctgggc tacgacttgc
    51361 aaagtggcag tccacaactc aatgcacatc gccagttgtt tcagcaggca ctgcattcac
    51421 cggtattaac caacctcaac gtctggtatg tgccagaagc agtaaaaact cgctatgcgc
    51481 atctgaatgc caactggctg gagatgaata atcggctcag caagggcgat ttgccgtggt
    51541 atcaggccaa tattaataat tatgttaatc agatagacct gttcgtgctg gctttacagc
    51601 actacgctga acgcaaaatg ctgctggtgg tggcgatttc cctggctggc ggtatcggta
    51661 ttttcacgct ggtctttttt actttgcgcc gcatacgcca tcaggtggtt gccccgctga
    51721 atcagctggt taccgccagt cagcgtattg aacacgggca gttcgactcg ccgccgctgg
    51781 ataccagtct gccgaatgag cttggtctgc ttgcaaaaac ctttaaccaa atgtcgagcg
    51841 agctgcataa attgtacctt tcgctggagg cgtcagtaga agaaaagacc cgcgatctcc
    51901 acgagaccaa gcgtcgtctg gaggtgttgt atcagtgcag tgttcccagg cgctgaacac
    51961 cagccagatt gatgtgcatt gtttccgcca tattttgcag attgttcgcg acaatgaagc
    52021 agctgaatat ctggagctga acgttgggga taactggcgg attagcgaag ggaagccaaa
    52081 ccctgagctg ccaatgcaga ttttaccggt gacaatgcaa gagacggttt acggcgaact
    52141 gcactggcaa aatagtcacg tttcatcatc agaaccgctg cttaacagtg tttcatcgat
    52201 gctgggacgc ggtttgtact ttaatcaggc gcagaagcat tttcagcagt tattgttgat
    52261 ggaagaacgt gcgaccatcg cccgcgaatt gcacgactcg ctggctcagg tgctttctta
    52321 cttacgtatc cagttgacgt tactgaagcg ttcgataccg gaagataacg ccaccgcaca
    52381 aagtatcatg gccgattttt cccaggcgtt gaatgatgct tatcggcagt tacgcgagct
    52441 gttgactact ttccgcctga cgctgcagca ggcggatctc ccctccgcgt tgagggaaat
    52501 gctggatacg ttacaaaatc aaaccagcgc taaactgacc ctcgactgcc gtctgccaac
    52561 cctggcactg gatgcgcaaa tgcaggtgca tttgttgcaa attattcgcg aagcggtgct
    52621 gaatgcgatg aagcacgcca acgccagcga aatcgccgtc agttgcgtca ccgcgccgga
    52681 cggcaatcac acggtttata tccgtgataa cgggattggt atcggtgaac cgaaagaacc
    52741 cgaaggtcat tatggactga atatcatgcg cgaacgcgcg gaacggctgg gcgggacgct
    52801 gactttttca caaccttccg gcggcggcac gttagtgagt attagctttc gctccgcaga
    52861 gggtgagggg aaggggaaca cgccggctga ggtgtaaata attaccctca caccggccct
    52921 ctccctttaa aggcgagagg gaaaaacgtg cccatattga cctgtaagcg agtgtgtcag
    52981 caatctcatg ataatttaca ttaactcctt tttttctcca cgattggctc gtaccttgcc
    53041 gctacagtga agcaagtcaa gcctacaaca atacgcagaa acacgaggtc ctcttttaat
    53101 ggcgaatttc tttattgatc gccccatttt tgcctgggtg ctgacaatcc tgttgtgtct
    53161 gacaggtacc ctggcgattt tttcattgcc cgttgaacaa taccccgatc tcgcgccacc
    53221 gaatgtgcga gtgaccgcta actatcccgg cgcatcggcc cagacgctgg aaaacaccgt
    53281 gacccaggtt atcgagcaaa atatgaccgg cctcgataat ctgatgtata tgtcatctca
    53341 gagcagtggc accggtcagg catctgtcac tttaagtttt aaagcaggca ccgatccgga
    53401 cgaagccgtg cagcaagtac aaaaccagct gcaatcagcc atgcgaaagt taccgcaggc
    53461 ggtgcaaaat cagggcgtga cggtgcgtaa aaccggcgat accaacattc tgaccattgc
    53521 cttcgtctct accgatggct cgatggataa acaggatatt gctgattatg ttgccagtaa
    53581 tattcaggac ccgttaagcc gcgtgaatgg cgtcggggat atcgatgcct atggttcgca
    53641 atattccatg cgtatctggc tggacccggc gaaactcaac agtttccaga tgacggctaa
    53701 agatgtcact gatgccattg agtcacagaa cgcgcagatt gcggttgggc aacttggtgg
    53761 tacaccttcc gtcgataagc aggcgctcaa cgccaccatt aacgcccagt cactgctgca
    53821 aacaccagaa cagttccgcg atatcacctt gcgggtgaat caggacggct cagaggtaag
    53881 gctgggcgat gtcgccaccg tcgaaatggg ggcggagaaa tacgattatc ttagccgctt
    53941 caatggtaag ccagcctccg ggttgggggt aaaactggcc tccagcgcaa acgaaatggc
    54001 gacagcggag ctggtgctca atcgtctcga cgagctggcg cagtatttcc cgcacggact
    54061 ggaatacaag gtggcgtatg aaaccacctc gtttgttaaa gcctccattg aagacgtggt
    54121 gaaaacgctg ctggaagcta tcgctctggt tttcctcgtt atgtatctgt tcctgcaaaa
    54181 cttccgcgcc acgctgatac ccactatcgc cgtgccggtg gtgttgatgg gaaccttctc
    54241 cgtactttac gccttcggtt acagcgtcaa caccttaacc atgttcgcga tggtgctggc
    54301 gatcggtctg ctggtggatg acgccatcgt ggtggtggaa aacgtcgaac gtattatgag
    54361 tgaggaagga ctcactcctc gcgaagccac acgtaaatcg atggggcaga tccagggggc
    54421 actggtcggg attgcgatgg tgctttcggc ggtatttgta ccaatggcct tcttcggcgg
    54481 caccaccggt gccatctatc gccagttctc tattaccatt gttgcggcga tggtgctgtc
    54541 agtactggta gcgatgctcc tcactccggc tctgtgtgcc acgctactta agccactgaa
    54601 aaaaggtgag catcacgggc aaaaaggttt ttttgcctgg tttaaccaga tgtttaaccg
    54661 caacgccgaa cgctacgaaa aaggggtggc gaaaattctc caccgtagcc tgcgctggat
    54721 tgtgatttat gtcctgctgc ttggcggcat ggtgttcctg ttcctgcgtt tgccgacgtc
    54781 gttcttaccg ctggaagacc ggggcatgtt tactacctcg gtacaattgc ccagcggttc
    54841 aacccaacaa cagaccctga aagtcgttga gcaaatcgag aaatattact tcacccatga
    54901 aaaagacaac atcatgtcgg tgtttgccac cgttggttct ggccctgggg gtaacgggca
    54961 aaacgtggcg agaatgttta tccgcctgaa agactggagc gaacgcgaca gtaagaccgg
    55021 cacctcgttt gccattatcg agcgtgcaac gaaggcgttt aaccaaatta aagaagctcg
    55081 cgttatcgcc agcagcccac cagcaattag cggtcttggt agttctgcag gttttgatat
    55141 ggagttgcag gaccacgctg gagcgggtca cgatgcgctg atggcagcac gtaatcagtt
    55201 gctggcgctg gcggcggaaa acccggagct aacccgtgtg cgccataacg gcctcgatga
    55261 cagtccgcag ttgcagattg atatcgacca gcgtaaagct caggcgctgg gcgttgatat
    55321 cgacgatatt aacgacacac tgcaaaccgc ctggggttcg agctatgtga atgactttat
    55381 ggatcgcggt cgcgtgaaga aagtctatgt gcaggcagct gcgccgtatc gcatgctgcc
    55441 agatgacatc aatctctggt atgtccgaaa taaagatggc ggcatggtgc ccttctctgc
    55501 tttcgcgacc tcacgctggg aaacaggctc gccgcgtctg gaacgctata acggttattc
    55561 tgcggttgag attgttgggg aagccgcacc gggggtcagt accggtacgg cgatggatat
    55621 tatggaatcg ttagtgaagc agctgccaaa cggctttggt ctggagtgga cggcgatgtc
    55681 gtatcaggag cggctttccg gcgcgcaggc tccggcgctg tacgccattt ccttgctggt
    55741 ggtattcctg tgtctggcgg cattgtatga aagctggtcg gtaccgttct cggtaatgct
    55801 ggtcgtgccg ctgggggtaa tcggcgcgct gctggcaacc tggatgcgcg ggctggaaaa
    55861 cgacgtttac ttccaggtgg gcctgttaac ggtcattggt ttatcggcga aaaacgccat
    55921 cctgatcgtc gagtttgcta acgagatgaa ccaaaaaggc catgacctgt ttgaagcgac
    55981 gctccacgcc tgccgtcagc gtttacgccc gattctgatg acctcgctgg catttatctt
    56041 cggcgtattg ccaatggcaa ccagcacggg tgccggttcc ggtggtcagc atgcggtggg
    56101 tactggcgta atgggcggga tgatttcagc tactattctg gctatttact tcgtgccgct
    56161 gttctttgtg ctggtgcgcc gccgcttccc gctgaagccg cgcccggaat aagcaataaa
    56221 aaaggcgaca tgccaatgtg tcgccttttt caactttccg attaagaacc tgctcagcgg
    56281 gttcttgctg tttatacttt gtctcaggaa ttacttacga agcataactt cgataaagtc
    56341 tttccagttc cccagttcac gttcaatcat aacaacctct cttataatta tgggtattct
    56401 acggaaacaa tataccgtgg tgaagctaat ttactcgatt gctgcgatga ctacctccgg
    56461 gggacaaacc ttatgtaaat actatggtcc tgcggtgata atttgtatgt gatacacagc
    56521 aacatttcga gatattcata cggcatctaa tactgattta attctggtta aaatacagac
    56581 agataacaag atgaatattc ttaatgttta cgttaaaaat gtttaatatt atttaatagt
    56641 tgttaatttg aatacttcga taatgttata ttttctgata atcatttgca ggcaaaatgt
    56701 tttcaccctt aaatgagtat ttattctcat aaatcgaaaa aggattcatt atggttacac
    56761 tttacggcat caaaaattgt gacaccatta aaaaggctcg ccgttggctg gaagccaata
    56821 acatcgacta tcgttttcat gattaccgcg tcgatgggct ggacagcgaa ttattgaacg
    56881 gttttatcaa cgaattaggc tgggaagcgt tactcaacac ccgtggtaca acctggcgta
    56941 aactggacga aactacccgc aataaaatca ccgatgcggc ctctgcggcg gcattaatga
    57001 ctgaaatgcc tgcaattatc aaacgtccat tgctctgcgc gcctggtaag cctatgctgc
    57061 tgggtttcag tgattccagt tatcagcaat ttttccatga ggtgtagtct atgtcgtgcc
    57121 cggttattga gctgacacaa cagcttattc gccgcccttc cctgagtcct gatgacgcag
    57181 gatgtcaggc tttgttgatt gaacgtttgc aggcgatcgg ttttaccgtt gaacgcatgg
    57241 attttgccga tacgcagaat ttttgggcat ggcgtgggca aggtgaaacg ttagcctttg
    57301 ccgggcatac cgacgtggtg ccgcctggcg acgccgatcg ttggatcaat ccgccgtttg
    57361 aacccaccat tcgtgacggc atgttattcg ggcgcggtgc ggcagatatg aaaggctcgc
    57421 tggcggcgat ggtggtagca gcagaacgtt ttgtcgcaca acatcccaac catacggggc
    57481 gactggcatt tctgatcact tctgatgaag aagccagtgc ccacaacggt acggtaaaag
    57541 tcgtcgaggc gttaatggca cgtaatgagc gtctcgatta ctgcctggtc ggcgaaccgt
    57601 cgagtatcga agtggtaggt gatgtggtga aaaatggtcg tcgcggatcg ttaacctgca
    57661 acctcaccat tcatggcgtt caggggcatg ttgcctaccc acatctggct gacaatccgg
    57721 tacatcgcgc agcacctttc cttaatgaat tagtggctat tgagtgggat cagggcaatg
    57781 aattcttccc ggcgaccagt atgcagattg ccaatattca ggcgggaacg ggcagtaaca
    57841 atgttattcc gggtgaactg tttgtgcagt ttaacttccg cttcagcacc gaactgactg
    57901 atgagatgat caaagcgcag gtacttgccc tgcttgaaaa acatcaactg cgctatacgg
    57961 tggattggtg gctttccggg cagccatttt tgaccgcgcg cggtaaactg gtggatgcgg
    58021 tcgttaacgc ggttgagcac tataatgaaa ttaaaccgca gctactgacc acaggcggaa
    58081 cgtccgacgg gcgctttatt gctcgcatgg gggcgcaggt ggtggaactc gggccggtca
    58141 atgccactat tcataaaatt aatgaatgtg tgaacgctgc cgacctgcag ctacttgccc
    58201 gtatgtatca acgtatcatg gaacagctcg tcgcgtgatg agtggttctg caagaggaaa
    58261 taagcatgga ctggctggct aaatattggt ggattctggt gattgtcttt ttggtaggcg
    58321 tcctgctgaa cgtgattaaa gatctcaagc gcgtcgacca taagaaattt ctcgccaaca
    58381 agccggagct tcccccgcat cgtgatttca acgataagtg ggacgatgac gacgactggc
    58441 cgaaaaagga tcaaccgaag aagtaagctg agtgctcaac cgtaggccgg ataaggcgtt
    58501 aacgccgcat ccggcaatgg tgaacgatcc ctgatgcgac gctcacgcgt cttatcaggc
    58561 caggtaatct gtcttttaca tcatttcaat cacatcatca tcgccaggct taccgccact
    58621 tagcgcttgg tcgaaataat gtttcggaat ggtataacgc aagtgatcca gggcaaattg
    58681 catactgcga ttgtcgatcg catggcctaa atcctccacg atatccagcg tcacgtcacc
    58741 accggcactg attaacgctt cctgcgccgc aactgcatgc gccagatcga taaccggatc
    58801 ttcaccacca tgaatcaagt gaattgtcgt ggctgtagac gcggtctccg gcaggctcgc
    58861 atagcgtccg ttaaaggcaa tgacgcgcga agcaaggccc ggttcggctt taatgctctc
    58921 cagcgccata atcgcgcctt gtgagaaacc aattagcgcc gtggcgttag cccctactcc
    58981 gctctgtttc tgccagtagc gtaccgtttc aataaacgtc ggcatgatgg catccacacg
    59041 cgcctggcga ttatcttccg taataccctg cactgaaaac cactgacgcc ccgccggatt
    59101 accgctcggc tccgcgccac cgacactcac caccagcgca tcaggaaaca gcggtgcaaa
    59161 ccagctgcct atttccccca ttgccaccgg gttatccccg acaccgtgga aaagcagtaa
    59221 caactgttgt gcaggtttat ccgggctttg aacaacaaaa tggtcatgtt tcatggcgaa
    59281 ctccttaact gatgtcatta attttacgcc gctgactcac aatgaccatg ggagaaaatt
    59341 gaaggagtca gtgaaaaaat tgccattgcg ttatgcgatc gcgcagacgc tcagtgcgaa
    59401 cctcatttag tgcgaacagc gcctgcgcgg cctcttcccg ctggcgaacc agtaacatct
    59461 tgcggcctga aagtttaagt gtggtacata actgcgcgtc actggcgttt ttctgtaaac
    59521 gcccacgcag cgccggtaat gccagttcac tggtttgtag cagacgcaat aagcaaccta
    59581 acgatgttaa tagcggacga tgagcgaaag caaaaccggc cagttcaagc cagtcgtcgt
    59641 cagaaaggat ggcatcgttt agtggatcaa caggaagcat ttcgccattc cactgcgcga
    59701 gagcttgcgc atcgcgacgt aaacggtaat gctcacgttc agccagctgt ttacccgcat
    59761 cactcatcgg taacagcgcc atcgccgtat agcaaccgct gctggcttcc cgatgattac
    59821 ccatccgcac cagcacaaaa ccgcagcgtt gccagaaacg ccataactcc ccagtgtaac
    59881 caaaactcac cgaaagatag tcgaggtcat gcgtatattg caaagctcca gcaataagtt
    59941 gtcgccctgt gccttcccgc tgacgagccg gatgaactgc tatccggcta acccgccttc
    60001 cgcgcaatgt cgccgccagt ggattgctgc cgtgcgccgc cagcgactgg gccaccagat
    60061 taccccgcgg gcgacgaaaa cctgcccata ccgcctgact gagttcttga gataatccgc
    60121 cctcatccac cagccacaac gccccggcaa tctcgttttc gccagccgcc tgtaaaaaat
    60181 gttgccctgg tgcatccatc atccggcgta aatccagcgg cgaggtccgg tagtgcgcac
    60241 cagacaataa ctgataaacc tttaacggcg tttctggctc gcttcgccat aacgtttgtt
    60301 caaatgcgga aatgacgata ttgccttgtg gtgtatgggt gaagttttca tcgttaaaaa
    60361 ccagtgcctc actaaccatt ttttccagcg ggcatccctg tgcccagcgg atcggctgtt
    60421 gcagttcaaa acggtgtaaa tgcggaaagc gagcgcaaaa tttcagcaaa aaaccacgtc
    60481 cggtaccttc gtagccctgc accgtagtgg ttaacaacgt tcgaggaaaa cgcgatacca
    60541 gttgatgcaa caacggcgca ggtatggctg cggcttcgtc gaccaccagc cagtcggctt
    60601 gctcatcgct ggctaacaag gcatccggtg caataaagcg aaacttctcg cccgcaaatt
    60661 gtgccagtac atccgttgcc gcttttgcgg gcgcggtgac aatcgcactg cccgcaatac
    60721 gagaaatgag ttgccctgcc aacgccgact taccgcgccc acgcgcagcc gttaccgctg
    60781 ccacgcccgg cggcatggtc agtagctgct gtaagagttg ctgttgttct ggttgtggtg
    60841 cgccggtcgc ggggtgccag tcagtacggg gagtaaaatg cgccaacgag aacggctggt
    60901 tttgccgcca gaggatagcg tcgttatccg ccgtaagtac gcgtttgaga tgctggacaa
    60961 aatgcggcgt cgcaataggg tcagggcaat cactccagcg cagcgagtcg gcatcaggtt
    61021 ggttttccca ctcttcccat acagggagta acaaaaccag ccagcttccc gctttcaacg
    61081 ttccgctaag tgctgcaaag gcagcggcat caaagccgtg gcgggcgtcg aataccgcat
    61141 gccggaactc gcgcccaagt aaagtttgta gcgccgaggg tgaacagtgg ttttcagcat
    61201 ctagctgcgg cgaaatccac agccagtcgc caggtaaggc atcacgcaac ttaagagcat
    61261 gatcaaaaca ccaaccctct tccccgctca acaccagcaa gcggcagatc ccttcacgtt
    61321 tcatttgcgc tgttaatgtg taaagcgcag tcagttcagc catccctgcc ccggaaatta
    61381 aatgctttta ccaaaagtat tgcattgtgc cggatcgccg ctgtcgaaac cacgtttaaa
    61441 ccagctgtag cgttgctgag aagtgccatg tgtgaaactg tctggtacta ctcgcccctg
    61501 actttgctgt tgtaaacggt catcgccgat ggcctgcgcc gcattcagcg cctcttccag
    61561 atcgccagtt tccagaacgc cttgctgctg catactatgc ccccagacac cggcaaaaca
    61621 gtcggcctgg agttccatac gcacagataa gcggtttact tccgcctgcg tcgcgttttg
    61681 ttgcagttga cgaactttcg gctcgatgcc taacagtttc tgcacatgat gaccgacttc
    61741 atgggcgata acgtacccct gggcaaaatc gccatccgcg ccaagtttgt ctttcatgtc
    61801 atcatagaag gagagatcga tataaactgt gccatccgcc gggcaataga acggccccat
    61861 aatggactgg cccgccccgc agccggtgcg cgtcattcca cggtacatga ccagcttcgg
    61921 ttgctgatag gtcttaccca tcttttcgaa ctgttgtccc caggtgtctt ccgtggttgc
    61981 ctgaatcacc gaggtgaatt ttgcggcttc gtcttcatta gggctaattg accgcgttga
    62041 ttgttgttga gaaaccggct gcccggtcat caacccggtt aaatcaacgc catagtagcc
    62101 tgcaaccagc acgactatca gtaaaatcag cccgccttta ccgctcggca gacggaaacc
    62161 gggaccgccc attgacggac cgccagagct gttgcgcctg tcttcaacat tgtcactttc
    62221 acgtcgccct tgccaacgca tagatacctc aacaatatat tcattataga aatgatcgta
    62281 ggtggttcag aggaagatta ccacaggaaa gcgggatgaa atggcggagg aatgagcgtt
    62341 caatgcatat attccaagag cggttgtctt gccgtgccag ctgcacggca agatgatgaa
    62401 cagaaaaatc agtccagctg tacacccagg cggcgggcga cggcttcata ggcttcgatc
    62461 aggccaccga ggctctggcg gaaacggtct ttgtccattt tctccagtgt ttctttgtcc
    62521 cacaggcggc tgccgtccgg ggagaactca tcgcccagta ccacttcacc tttgtacaga
    62581 ccaaactcca gcttgaagtc gaccagaatc agaccagcat catcgaacag ttttttcagc
    62641 acgtcgttcg ctttgtaggt cagctctttc atacgcgcca ggttctcttt gctcacccag
    62701 ccaaaggttt cgcagtaaga ttcgttgacc atcggatcgt gcatggcatc gtttttcagg
    62761 aacagatcga acagcggcgg gttcagatca ataccttctt cgattccaag acgtttcact
    62821 agagagccag cagcacggtt acgcacgaca cactcaaccg gcaccatatc cagctttttc
    62881 accagacatt cggtatcgga gagcagacgc tccatttgag tcgggatacc cgcttcagcc
    62941 agtttgctca taatgaagta gttgaacttg ttgttcacca tacctttgcg atcaaactgc
    63001 tcaatgcgcg cgccatcccc tgctgacgta tcattgcgga attcgagcac caacaggtcc
    63061 gggttttccg tgctgtatac ggttttcgct ttaccacgat acaactcagc ttgcttttgc
    63121 atctttatca ctcctgggtg tgaattaacg ttttaaaatc ttttgctgtc tggtgtgccg
    63181 gatgttttgt cggatgcggc gtgaacgcct tatccgaact acgtccgaca aacaaatttc
    63241 gtgcgaatta ccgctaaaat cgcgttttcc tgccgacgca cacgtttgcg tatcatatca
    63301 gaaaaaaggg ccggatgatt ccagccctgt atttttactt gctaaacgca gcctggaaga
    63361 cagctaccag cgcgtcgttc tgactctgag tcagagtatg acctttcgga tcgatgaact
    63421 gtaggctgct gcggttatct aaatcgccaa cctgcagttt atagtcaccg gatgccaggc
    63481 ctggatcgct cgcgcccagt tcctgccagt cgctgtcaga cagcggctta taagttacgg
    63541 ccatgttgcc ctgcgaacgg gtgctgtcgg tcactttcat gcccactttt tccagcgccg
    63601 ctggcagacg ttgccaaacc acattgaacg gcccgcgtac gaccagcatt ggtaaaccgg
    63661 tgtcatcagc tgcactttgt acgtccatag tggtggaggc acgattttgc gcagcgttcg
    63721 cggcgtcagt ggcagattta tccagaccgg cggaaataac gttcatcatc tccgtgctgt
    63781 aacgctgcat ggaagccgcg tctgcaaccg gtttgcccgc ctgttccagg ttcagcagtt
    63841 taaccgtaac cgcctgctga taaccctgcg gcttaacaga gatttgataa cgaccacgat
    63901 actgctcgtc ttcgtccaga cggttccatt gtacccaatc ggtggtcagt gtctgaccag
    63961 catcatcacg ttgggtgatg gtgtagtttt tcgcctgcag cacgctaacc acctgcggcc
    64021 acagagtatt gccacgacca ttttccacca gcaatgaagc ggtatcgccc gtgaactggg
    64081 tacgcgcgcc agaaaccagt gccaacggct gggctggtgg acgaatgtcc agcgccttac
    64141 cgacagcacc actaccgttg gtcaccggga ttgcataatc accggaggtc accggcaaaa
    64201 tcattccagc cggggcatga agctccgcaa gcggtgccgc ttccaggtag gcttcatcac
    64261 cactgacctg acgcttatag cgtgagtcag aactacaggc agcgagtaat aaaacaagcg
    64321 aaacacccgc aacctttgcc aggcgcgact tttgaacaga gtaagccatc aaatctccct
    64381 aaactttaca gcaaaccggc atgcttaagc gccgctctga ccgtctcacg accactgtcg
    64441 gtgattggtg tcattggcag gcgcagcgta tcggtcgcca caagacccag ttccttacat
    64501 gcccatttca ccgggattgg attgggttcg acaaatagtt tgttgtgtaa tggcatcaga
    64561 cgctgattaa taacgcgtgc ctcggcaaaa tgcccttctg ctgccagttt gcacatctgg
    64621 gccatatcac gcgctgcgac gttagccgta acggaaataa ctccatgacc gcccaattgc
    64681 atgaagtcca gcgcgctcgc atcatcgccg ctcagcagaa caaaatcatc tgaaaccagc
    64741 tctttgatct ggtttacacg cgttaagttc cctgttgcct ctttgattcc gataatattt
    64801 tttactttcg ccagacggcc caccgtttcc gggagcagat cgcagccagt acgggacggc
    64861 acattataca gaatttgcgg caggtcagta tgctcagcga tggctttgaa atgctgatac
    64921 aaaccttctt gcgacggacg attgtagtaa ggggttaccg tcaggcagcc gacgatacca
    64981 ctgtcattga agcgctgcgt caggctaatg gcttccgcag tagcgttagc gccggtcccg
    65041 gcaattaccg gaatgcgccc atcagccaga tccagcgtca tcatcaccac atcagcatgt
    65101 tcgtcatgat ttaaggtagc ggactcgcca gtggtgccaa cagaaacgat cgccgaagta
    65161 ccgctggcga catgataatc aatcagtttt ttcaagctag cccgacagac attacctttt
    65221 tcatccatcg gagtaacaat cgcgacaata cttcccgtga acatgggcca tcctctgtgc
    65281 aaacaagtgt ctcaatggta cgtttggtat ggcattaaaa gcaagcagac agaaccgttc
    65341 tgattgttgt atgcatgatt tttttatgct ttccttaaga acaactcacc ccttaaagga
    65401 ataaccagtt tgacactgtc atcgcaacat tatctggtga tcactgcgtt gggtgccgat
    65461 cgccctggaa ttgtgaacac catcacccgt catgtcagta gttgcggctg taatattgaa
    65521 gacagtcgcc tggcgatgct gggagaagag ttcacgttta tcatgctgct ttccggttca
    65581 tggaatgcca tcactctgat tgaatcaacg ttaccgttga aaggtgccga actggatctt
    65641 ttaatcgtga tgaagcgcac gacggcgcgt ccgcgtccgc caatgccagc atctgtctgg
    65701 gttcaggtcg atgttgcaga ctccccgcat ttaattgaac gcttcacagc acttttcgac
    65761 gcgcatcata tgaacattgc ggagctggtg tcgcgcacac aacctgctga aaatgaacgg
    65821 gctgcgcagt tgcatattca gataaccgcc cacagccccg catctgcgga cgcagcaaat
    65881 attgagcaag cgttcaaagc cctatgtaca gaactcaatg cacaaggcag tattaacgtc
    65941 gtcaattatt cccaacatga tgaacaggat ggagttaagt aatgaatcca ctgaaagccg
    66001 gtgatatcgc accgaaattt agcttgccgg atcaagacgg agaacaagtt aatttgaccg
    66061 acttccaggg acagcgtgtt ctggtttatt tctacccgaa agccatgacc cccggctgta
    66121 ccgtacaggc ctgcggctta cgcgataaca tggatgagtt gaaaaaagcg ggcgttgatg
    66181 tgctgggtat cagcaccgat aaacccgaaa aactctcccg ttttgcggaa aaagagctgc
    66241 ttaactttac gctcctgtct gatgaggacc accaggtgtg cgaacaattc ggcgtctggg
    66301 gtgaaaagtc cttcatgggc aaaacctacg atggtattca tcgcatcagc ttcctgattg
    66361 acgctgatgg caaaatcgaa catgtctttg acgatttcaa aaccagcaat caccacgacg
    66421 ttgtgctgaa ctggctgaaa gaacacgcct gattactttg ctccattccg tgctggctgc
    66481 gcttgcggcc agcatacctc acttctcgtg atcaagatca cattctcgct ttcccctgcg
    66541 acacgggtgt cgaatccatt ttttgctgaa cgttaatgac catcattttt gtaccgttca
    66601 gaatccagtt aatacataac ttattgaata gattgagtta atcagaatgg catcctttat
    66661 acaatatgaa atgcaatgtt tcatatcatt ttcaaggagc cgacatgaac cgctttgtgg
    66721 tggccgaacc actgtggtgt acaggatgta atacctgtct cgctgcctgt tcggacgtgc
    66781 ataaaacgca aggtttacag caacacccgc gcctggccct ggcgaagacg tcaacaatca
    66841 ctgcccctgt cgtgtgtcat cactgtgagg aagccccttg cctgcaggtc tgcccggtca
    66901 atgccatctc tcagagggat gatgcgatcc aactcaacga aagcctctgt attggctgca
    66961 agctttgcgc cgtggtctgc ccatttggcg caatcagcgc ttcaggaagc cgtccggtga
    67021 atgcccatgc gcaatatgtt tttcaggctg aaggctcact caaagacggc gaagaaaacg
    67081 tgccgcccca acatgcgttg ctgcgctggg aaccgggtgt ccagaccgtc gcggtgaaat
    67141 gcgacctgtg tgatttcttg ccagaaggtc cggcctgcgt tcgcgcttgc ccgaatcagg
    67201 cgttacggct gatcaccgac gatagcctgc aacgtcagat gaaagaaaaa cagcgccttg
    67261 ccgcaagctg gtttgccaat ggcggggagt ctcccctttc cctcactcag gagcaacact
    67321 aatggatgcc ctgcaattat taacctggtc gctgatcctc tatctgtttg ccagtctggc
    67381 ttcgctgttt ttactcggtc tggacagact ggctattaag ctttccggca tcacatcgct
    67441 ggtgggcggc gtgattggca tcatcagcgg aattacgcaa ttacatgctg gtgtaacttt
    67501 agtcgcccat tttgccaccc cttttgactt tgccgattta accctgcgaa tggatagcct
    67561 ctcggcattt atggtgctag ttatctcctt gctggtggtg gtttgttcgc tctattcatt
    67621 gacttatatg cgcgaatacg agggcaaagg cgcggcggcg atgggcttct ttatgaatct
    67681 tttcatcgca tcgatggttg ccctgctggt gatggacaac gctttttggt tcatcgtgct
    67741 gtttgaaatg atgtcgctgt cttcctggtt tctggtcatt gccaggcagg ataaaacgtc
    67801 gatcaacgct ggcatgctct acttttttat cgcccacgcc ggatcggtgc tgattatgat
    67861 cgccttcttg ctgatggggc gcgaaagcgg cagcctcgat tttgccagtt tcgcacgctt
    67921 tcactttctc cggggctggc gtcggcggtg tttctgctgg cctttttcgg ttttggcgcg
    67981 aaagccggga tgatgccgtt gcacagctgg ttgccgcgcg ctcaccctgc cgcaccatcg
    68041 cacgcttcgg cgttgatgtc tggcgtaatg gtcaaaatcg gtattttcgg catcctgaaa
    68101 gtagcgatgg atctgctggc gcaaacgggt ttgcctctgt ggtggggcat tctggtgatg
    68161 gcgatcggcg caatctccgc gctcctgggc gtgctatatg cgctggcgga acaggatatc
    68221 aaacggctgc tggcctggag taccgtcgaa aacgtcggca ttattttgct ggcagtcggt
    68281 gtggcgatgg acggtctgtc actgcacgac ccgctgctca ccgtggttgg actgctcggc
    68341 gcactgtttc atctgctcaa ccatgcgctg ttcaaagggc tgctatttct cggcgcggac
    68401 gcgattattt cgcgtttgca tacccacgac atggaaaaaa tgggggcact ggcgaaacgg
    68461 atgccgtgga cagccgcagc atgcctgatt ggttgcctcg cgatatcagc cattcctccg
    68521 ctgaatggtt ttatcagcga atggtacacc tggcagtcgc tgttctcact aagtcgtgtg
    68581 gaagccgtag cgctacaact tgcgggtcct attgctatgg taatgctggc agtcactggt
    68641 gggctggcag taatgtgctt cgtcaaaatg tacggtatta ctttctgtgg tgcgccgcgc
    68701 agtacacacg ctgaagaggc acaggaagtg ccaaatacga tgatcgtcgc catgctactg
    68761 ctcgcggcac tctgcgtatt aattgcgctt agtgccagtt ggctggcacc gaagataatg
    68821 catattgccc atgcgtttac caatacccct cccgtcactg tcgccagcgg aatagcactt
    68881 gtacccggca cgtttcatac acgggtcact ccttcattac tgttgctgtt actactggcg
    68941 atgcctttgc tgccaggcct ttactggctg tggtgtcgtt cgcgccgcgc agcgtttcgt
    69001 cgcacaggag atgcctgggc atgcggctac ggctgggaaa acgcgatggc tccgtcaggc
    69061 aatggcgtga tgcagccgct gcgtgtggtc ttctctgcgt tatttcgtct acgacaacag
    69121 ctcgacccta cgctgaggct aaataaaggt cttgcgcacg tcaccgccag ggctcagagc
    69181 acagaaccct tctgggatga gcgggtgatc cgccccatcg tgagcgccac ccaacggctg
    69241 gtcaaagaaa tacagcatct gcaaagcggc gactttcgtc tctattgcct gtatgtggtc
    69301 gccgcactgg ttgtgctgct aatcgctatt gccgtctaag gaaatcacca tgagacaaac
    69361 tctttgcgac ggatatctgg tcatttttgc gttagcacag gccgtgattc tgctgatgct
    69421 aaccccactt tttacgggta tttcccggca gatacgcgcg cgtatgcact cccgccgcgg
    69481 gccggggatc tggcaggatt atcgcgatat ccacaaactg tttaaacgcc aggaagttgc
    69541 gccgacatct tcaggtctga tgttccgcct gatgccgtgg gtattaatca gcagcatgct
    69601 ggtgctggcg atggccttac cactatttat taccgtttcc ccttttgcgg gcggcggcga
    69661 tctgatcacc cttatctatc ttcttgccct gtttcgtttt ttctttgctc tttccgggct
    69721 ggataccgga agtccgtttg cgggagtcgg tgccagtcgc gagttgacgc tcggcattct
    69781 ggtcgaacca atgcttattc tctcactgct ggtattggcg ctgatagcag gttccacgca
    69841 tatcgagatg atcagcaata cgctggctat gggctggaac tcgccgctaa ccaccgtact
    69901 ggcgttactg gcctgtggtt ttgcctgctt cattgagatg ggaaaaattc cctttgatgt
    69961 tgctgaagca gaacaggaat tacaggaagg cccgctgacc gaatattccg gtgccgggct
    70021 ggcgctagcg aaatgggggc tggggctgaa acaggtcgtg atggcagcac tgtttgtggc
    70081 cctgtttctg ccctttgggc gcgcgcaaga actttctctc gcctgcctgc tgacttcact
    70141 tgtcgttacg ctgctcaagg ttttgctgat ttttgtactg gcctcaatcg cagaaaacac
    70201 gctggcacgc gggcgttttt tactcattca ccatgtgacc tggcttggct tcagccttgc
    70261 tgcgcttgca tgggtctttt ggttaaccgg tctgtaagga gcactgacgg aatatggaaa
    70321 atcttgctct gacgacgtta atgctgcctt ttatcggcgc actggtcgtt tcgttttcgc
    70381 cacaacgtcg ggccgccgaa tggggggttt tgttcgccgc gctgaccacg ctgtgcatgt
    70441 tgtcgctgat ctccgcgttt tatcaggccg ataaagttgc cgtcacgttg acgttggtca
    70501 acgtggggga tgtggcatta tttggcctgg tcattgatcg cgtgagtacg ctgattctgt
    70561 ttgtggtggt gtttctcggt ttgctggtca cgatctactc cacgggttat ctgacggata
    70621 aaaatcgcga acacccgcat aacggcacga atcgttatta cgcatttttg ctggtgttta
    70681 tcggcgcgat ggcgggactg gtactctcct cgacgctgct cggtcagttg ttgttttttg
    70741 aaattacggg cggctgctcc tgggcgttga tcagttatta ccagagcgat aaagcgcagc
    70801 gttcagcact aaaagcgttg cttatcaccc atatcggttc gttggggttg tatcttgccg
    70861 ccgccacgct gtttttgcag accggaacgt ttgtgcttag cgcgatgagc gagttacatg
    70921 gcgacgcacg ttatctggtt tatggcggca tcctgtttgc cgcgtggggg aaatcggccc
    70981 agctaccgat gcaagcgtgg ctaccggacg caatggaagc gccaacaccg atcagcgcct
    71041 atctccacgc cgcatcgatg gtgaaagtgg gcgtttacat ttttgcccgc gccattatcg
    71101 acggcggcaa tatcccgcat gtgattggcg gcgttggcat ggtcatgaca ctggtcacca
    71161 ttctttatgg ctttctgatg tatttgccac agcaggatat gaagcggttg ctggcctggt
    71221 cgaccatcac tcaacttggc tggatgttct tcggcttgtc gctctccatc ttcggctcgc
    71281 ggctggcgct ggagggtagc atcgcctaca tcgtcaacca cgcgttcgct aaaagcctgt
    71341 ttttccttgt agcaggtgcg ctgagttaca gctgcggcac gcgcttgttg ccgcgtctgc
    71401 gtggcgtatt gcacaccctg ccgttgccag gcgtgggttt ctgcgtggca gcgctggcaa
    71461 ttaccggtgt gccgccgttc aacggcttct tcagtaaatt cccgctgttt gccgccggtt
    71521 ttgcgttgtc agtggagtac tggatcctgc tgcccgccat gattctgctg atgattgaat
    71581 cggtcgccag tttcgcctgg tttattcgct ggtttggtcg cgtcgtgccg ggcaaaccga
    71641 gcgaggccgt cgccgatgcc gcaccgctgc cagggtcaat gcgcctggtg ttgattgtac
    71701 tgattgtgat gtcgctgatt tccagcgtaa tcgccgcgac ctggttgcag taaggagatg
    71761 atgaatgacc ggttctatga tcgtaaataa tctggcggga ctgatgatgc tgacatcgct
    71821 gtttgtgatt agcgtcaaaa gctatcgcct gtcatgcgga ttttacgcct gccagtcgct
    71881 ggtgctggtg tctattttcg ccactctctc gtgcctgttc gccacagagc aactgctgat
    71941 ctggtccgcc agcgccttta tcaccaaagt gctgctggta ccgttaatca tgacttacgc
    72001 tgcacgaaat attccccaga acatcccgga aaaagcgtta ttcggtccgg caatgatggc
    72061 actgctcgcg gcgttaattg tcctgctttg cgcatttgtc gttcagcccg tgaagctacc
    72121 gatggctacc gggctgaaac cggcgctggc ggtagcgtta ggtcattttc tgcttggcct
    72181 gctgtgcatt gtcagccaac gcaatatcct gcggcaaatt tttggttact gcctgatgga
    72241 aaacggctcc catctggtgc tggcgcttct tgcctggcga gcaccggaac tggtggaaat
    72301 aggtatcgct accgacgcca tcttcgccgt cattgtgatg gtgttactgg caagaaaaat
    72361 atggcgtacc cacggcacgc tggacgtgaa caacttgacc gcgctgaagg gataatgaga
    72421 tgagttattc tgtgatgttc gctttactcc tgctcacgcc gctgcttttt tcgctgctct
    72481 gttttgcctg ccggaaacgg gggctctctg cgacttgtac ggtgactgta ttacatagct
    72541 tagggatcac actgctgctg attctagcac tctgggtggt ccaaactgcc gctgatgcag
    72601 gagaaatatt cgctgcggga ctgtggcttc atattgatgg tctgggcggt ttgttcctcg
    72661 ccattcttgg tgtgattggc tttctcaccg gtgtttactc gattggctac atgcgtcatg
    72721 aagtggcaca cggcgagctt tcacccgtta cactgtgcga ttactacggt ttcttccatc
    72781 tgtttttgtt caccatgctg ctggttgtta ccagcaataa cctgattgtg atgtgggcgg
    72841 cgatcgaagc caccacctta agctcggcgt ttctggtagg catttacggt cagcgttcat
    72901 cgctggaatc agcatggaag tacatcatta tttgtactgt tggtgtcgct tttggtctgt
    72961 tcggtaccgt gctggtatac gccaacgccg ccagcgttat gccgcaggca gaaatggcga
    73021 tattctggag cgaggttctt aagcaatcgt ccttgcttga cccaacatta atgctgttgg
    73081 cctttgtgtt tgtgctaatt ggctttggta ccaaaaccgg gctatttccc atgcacgcct
    73141 ggctgccgga tgctcacagt gaagcgccga gtccggtcag cgccctgctc tccgccgtat
    73201 tgctgaactg cgcgctgttg gtgctgattc gctattacat cattatttgc caggccatcg
    73261 gcagcgattt ccccaaccgg ttgttgctca tcttcggcat gttgtcggtt gccgtggcgg
    73321 catttttcat tctggtacag cgggacatta agcgtctgct ggcgtactcc agcgtggaga
    73381 acatggggct ggtcgcggtg gcgctaggca ttggcgggcc gctgggaatt tttgccgcgc
    73441 tgctgcacac cttaaaccac agtctggcaa aaacgctgct gttctgcggt tccggcaatg
    73501 tactgctcaa gtacggcacg cgcgatctca acgtcgtctg tgggatgctc aaaatcatgc
    73561 catttaccgc cgtgctgttt ggcggcggtg cgctggcgct ggcgctggcg ctggcaggga
    73621 tgccgccctt caacattttt cttagcgaat ttatgaccgt taccgcagga ctggcacgta
    73681 atcacctgct gattatcgtc ctgctgttat tgctgttaac gctggtgctg gcgggcctgg
    73741 tacggatggc tgcgcgggtg ttaatggcga aaccgccgca ggccgttaac cggggtgatc
    73801 tcggctggtt gaccacctcg ccaatggtga ttctgctggt catgatgctg gcgatgggaa
    73861 cgcatattcc acaacctgtc atcaggatcc tggcgggcgc ttccactata gtcctctcag
    73921 ggacgcacga cctgcctgca caacgtagca cctggcatga ttttttgcct tcaggcaccg
    73981 catctgtttc ggagaaacac agtgaacgtt aattcatcgt caaatcgtgg cgaagcgatt
    74041 ctcgccgccc tgaaaacgca gttccccggc gcggtgctgg atgaagagcg acaaacgcct
    74101 gaacaggtca ccattacggt gaaaatcaat ctgctgcctg acgttgtaca ttatctttat
    74161 tatcaacatg atggctggct tccagtcctg tttggcaacg acgagcggac acttaacggt
    74221 cattacgcgg tttattatgc cctttcaatg gaaggggccg aaaaatgctg gatcgtggtg
    74281 aaggcactgg tcgatgccga cagtcgggag tttccgtcag tcacaccgcg cgtccctgcc
    74341 gcggtctggg gcgagcgaga aattcgcgat atgtacgggc tgattccggt tggcctgccg
    74401 gatcagcgtc gcctggtgtt gcccgatgac tggccggaag atatgcatcc gctgcgcaaa
    74461 gatgcgatgg attatcgact gcgccctgaa ccgacgacag attccgaaac gtatgcgttt
    74521 atcaatgagg gcaacagcga tgcgcgggtg atccctgtcg gcccgctgca tatcacctcc
    74581 tatgaaccgg gtcacttccg cttgtttgtg gatggcgagc aaattgtcga tgctgattac
    74641 cgcctgtttt atgtccatcg cggcatggag aaactggcag aaacgcggat gggctacaac
    74701 gaagtgacct tcttatcgga ccgcgtgtgt gggatttgcg gttttgccca cagtgtggcc
    74761 tataccaact cggttgaaaa tgcactgggg attgaggtgc cgcaacgagc acatactatt
    74821 cgctcgattc tgctggaagt cgagcggcta cacagtcatt tgctcaacct tggcctctcc
    74881 tgccattttg ttggttttga taccggcttt atgcaatttt tccgcgtgcg ggaaaagtcg
    74941 atgacgatgg cggaattgct gaccgggtcg cgtaaaacct acggtctgaa tctgattggt
    75001 ggtgttcgcc gcgatattct caaagagcaa cgtctgcaaa cgctgaaact ggtgcgcgag
    75061 atgcgcgccg acgtgtcgga gctggtagag atgctgcttg ctacgccgaa tatggaacaa
    75121 cgcactcagg gcattggcat tctcgaccga caaatcgccc gtgattatag ccctgtaggg
    75181 ccgctgatcc gcgcagtggt tttgcccgtg atttgcgctt tgatcacccc tacgccgact
    75241 acggcaatat tccaaaaaca ctgtttacct ttaccggcgg cgatgttttc tcccgcgtga
    75301 tggtccgtgt caaagagacg tttgattcgc tggcaatgct ggaatttgcc ctcgacaaca
    75361 tgccggatac cccactgctg accgaaggct ttagctataa acctcacgca ttcgcgctgg
    75421 gctttgttga agcgccacgc ggtgaagacg tgcactggag catgctcggt gataaccaaa
    75481 aattgttccg ctggcgctgt cgtgccgcca cctacgccaa ctggccggtg ttgcgttaca
    75541 tgctgcgcgg caataccgtt tctgacgcac cgctgattat cggtagcctt gatccctgct
    75601 actcctgtac cgaccgtgtg acgctggtag atgtgcgcaa gcgccagtca aaaaccgtgc
    75661 tgtataaaga gatcgaacgc tacggcattg atcgtaaccg ttcgccgctg aagtaaggac
    75721 agaagatgct gaagttactg aaaactatta tgcgcgccgg aaccgcgacg gtgaaatatc
    75781 ccttcgcgcc actggaggtc agcccaggct ttcgcgggaa accggacctg atgcccagcc
    75841 aatgtattgc ctgcggtgcc tgcgcctgtg cttgtccggc aaatgcgctg actatccaga
    75901 ccgacgacca gcaaaattcg cgcacctggc agctctatct ggggcgttgt atttactgcg
    75961 gacgttgtga agaagtgtgc ccgaccagag ccatccagct taccaataac tttgaactga
    76021 ccgtcaccca taaagccgat ctctataccc acgcgacgtt ccatctacaa cgttgcagcc
    76081 gttgcgaacg cccgtttgcc ccgcaaaaaa ccgtcgcact ggctgctgaa ttgttggcac
    76141 agcaacaaaa tgctccacaa aaccgcgaaa tgttatgggc gcaagcgagc gtctgcccgg
    76201 agtgcaaaca acgcgcaacg ctgatcaacg acgatacaga tgtaccgctg gtggctaagg
    76261 agcagctatg agtccagtgc ttacgcaata tgtcagccag cccatcacgc tggacgagca
    76321 aacgcaaaag atgaagcggc atttgctaca ggatatccgt cgctcggctt acgtttatcg
    76381 catcgattgc ggcggctgca acgcctgtga aatcgaaatt tttgctgcca tcacccccgt
    76441 attcgacgca gaacgttttg gcattaaggt tgtttcatca ccgcgtcacg ccgatatttt
    76501 gttatttact ggcgcagtca cccgggcgat gcgtatgcct gcacttcggg cgtatgagtc
    76561 tgcccccgat cataaaatct gtgtttccta cggcgcgtgc ggtgtcggcg gcggtatttt
    76621 ccacgatctc tacagcgtct ggggcggcag cgacaccatt gtccccattg atgtttggat
    76681 ccccggctgc ccgccaacac cggccgccac cattcacggt ttcgccgtgg cgctcggttt
    76741 gctgcaacag aagattcacg cggtggatta tcgcgatccc accggggtga ctatgcaacc
    76801 gttgtggccg cagatcccgc catcacagcg tatcgccatt gagcgagaag cgcggcggct
    76861 ggcgggctat cgtcaggggc gagaaatttg cgatcggctc ctgcgccatt taagcgacga
    76921 tcctacagga aatcgggtta acacctggtt gcgcgatgcc gacgatccac gtctcaatag
    76981 tatcgttcag caactctttc gcgtactccg ggggttacat gactgaagag tgcggggaaa
    77041 ttgttttctg gacgctgcga aaaaagtttg tcgccagtag cgacgagatg ccggaacaca
    77101 gctctcaggt aatgtattac tcgctggcta tcggtcatca cgttggcgtg attgattgtc
    77161 tgaatgtcgc cttccgctgc ccactgacgg aatacgaaga ttggcttgca ctggtcgaag
    77221 aggagcaagc ccgacgtaag atgctggggg tgatgacttt tggtgagatt gttattgacg
    77281 ccagccacac cgccctgttg acccgggcat tcgcgccact ggcagatgac gcgacgtctg
    77341 tgtggcaagc gcgtagcatt caattcattc atctgttgga tgaaattgtg ctggaaccgg
    77401 ctatctatct gatggccaga aaaattgcgt gagaaggatt tctcattaat aaggactgtt
    77461 gatggctatg tcagacgaag cgatgtttgc cccgccgcaa ggaataacaa ttgaagcggt
    77521 aaacggaatg ctcgcggagc ggttagcgca gaaacacggc aaggcgtctt tattacgcgc
    77581 cttcatcccg ctgccgccgc cgttcagccc ggtacaactt attgaactgc atgttctcaa
    77641 aagcaacttc tattaccgct accatgatga tggcagcgat gtgacggcaa caacagagta
    77701 tcagggcgag atggtcgatt attcgcgtta cgccgtcctt ctcggcagta gtggaatggc
    77761 ggagctacgc tttattcgta cccacggcag ccgttttact ccccaggatt gcacactgtt
    77821 taactggctg gcacgcatta tcaccccggt tctgcaatca tggctcaatg atgaagcaca
    77881 gcaggtggcg ctgcgtttgc tggagaaaga tcgcgatcat catcgggtac tggtggatat
    77941 cactaatgca gtgctgtcac atcttgatct cgacgatctg atcgctgacg tcgctcgtga
    78001 gatccatcat tttttcggtc tggcttcagt cagtatggta ctgggcgatc atcgaaagaa
    78061 cgagaagttc agcctgtggt gcagcgatct ttctgcctca cattgtgcgt gtctgccaca
    78121 caatatgcct ggcgacagtg tattgctgac acaaacgcta caaacccgac aaccgacctt
    78181 gacgcaccgt gcagatgatc tgtttctctg gcaacgcgac ccgttattac tcttacttgc
    78241 atctaacggc tgcgaatctg cgctccttat accgcttacc tttggcaacc atacaccggg
    78301 tgcattgttg ctggcgcata cctcttccac tctctttagt gaggaaaact gccagctact
    78361 acaacacata gccgatcgca tcgctattgc cgttggcaat gccgatgcct gccgtagaat
    78421 gaccgatttg caggaaagtt tgcagcaaga aaaccaccag cttagcgagc agctcctttc
    78481 gaatctgggc gtcggtgaca ttatctatca aagccaggca atggaagacc tgctccagca
    78541 ggtagatatt gtggcgaaga gcgacagtac ggtgttgatt tgcggtgaaa ccggaaccgg
    78601 caaagaggtg atcgccagag cgatccatca acttagcccg cgacacgaca agccgctggt
    78661 caaaatcaac tgcgctgcca tccccgccag tcttctggaa agtgagttat tcggtcatga
    78721 caaaggggcg tttactggtg cgattaatac ccatcgtggt cgttttgaaa ttgccgatgg
    78781 cggcacgttg tttctcgatg aaattggcga tctgccgtta gaacttcagc ctaaactgct
    78841 gcgcgtattg caggaacggg agattgagcg tctcggcggg agtagaacga tcccggtaaa
    78901 tgtcagagtc attgccgcca ccaaccgtga tttgtggcaa atggttgaag atcgccagtt
    78961 tcgcagcgat ctcttttatc gcctgaatgt cttcccactg gaattgcctc cgctgcgcga
    79021 ccgtccggaa gatatccctc ttttagcaaa gcatttcacg caaaaaatgg cgcgccatat
    79081 gaatcgttca attgacgcca tcccgaccga ggcgctacgc cagttgatgt cgtgggactg
    79141 gccgggcaac gtgcgcgagc tggaaaacgt gattgagcga gcggtactgc tgactcgcgg
    79201 taacagtctg aatttacatc ttaatgtccg acaaagccgt ttactgccga cgctaaatga
    79261 agattcagcg cttcgcagtt caatggcgta gttgctgcac ccgacgacgc cagagaatga
    79321 cgaagaagaa cgtcagcgca ttgttcaggt attgcgagaa accaatggca ttgttgccgg
    79381 gccccgtggc gcggcgacac gattagggat gaagcgcacc acgctgctgt cacgaatgca
    79441 gcgtctggga atctcggttc gcgaggtgtt gtaatctgct tttgcaggag tatgcatgag
    79501 aaacaaactc tctttcgact tgcagttgag cgccagaaaa gcggcaatcg ctgaacggat
    79561 tgccgcccat aaaattgccc gcagtaaagt gtcggtcttt ttaatggcga tgtccgctgg
    79621 cgtgtttatg gcgatgtccg ctggcgtgtt tatggcgatc ggatttactt tttacctttc
    79681 cgttatcgcc gatgccccgt cttcataggc attaacccat ctggtgggcg gcctttgctt
    79741 tacacttggc tttattttgc tggcggtttg cggcaccagc ctgttcacct cgtcggtaat
    79801 gacggtaatg gcaaaaagtc ggggcgttat tagttggcga acttggctga ttaacgcact
    79861 tctggtggcc tgcggtaatc tggcaggtat tgcctgtttc agtttgttaa tctggttttc
    79921 cgggctggtg atgagtgaaa acgcgatgtg gggagtcgcg attttacact gcgccgaggg
    79981 caaaatgcat catacattta ctgaatctgt cagcctcggc attatgtgca atctgatggt
    80041 ttgcctggcg ctgtggatga gttattgcgg acgttcgtta tgcgacaaaa tcgtcgccat
    80101 gattttgtcc atcaccctgt ttgtcgccag tggctttgag cactgtatcg ccaatttgtt
    80161 tgtgattccg ttcgccattg ccattcgcca tttcgcccct acctccttct ggcaactggc
    80221 gcacagtagc gcagacaatt ttccggcact gacggtcagc cattttatta ccgccaatct
    80281 gctcccggtg atgctgggta atattatcgg cggtgcagtg ctggtgagta tttgttatcg
    80341 ggctatttat ttacgtcagg aatcctgaaa aatcagcccg gcgaaacagt tcgtcgggct
    80401 atttatttac gtcaggaatc ctgaaaaatc agcccggcga aacagttcgt cgggctgaag
    80461 attattcttg cgcgatttgc ccatcgggcc aggcgtggat cacggctttg atcagcgtcg
    80521 ccaatggaat ggcgaagaat acgccccaga atccccacaa accaccgaag atcaccaccg
    80581 ataaaataat caccagcgga tgcaggttaa ccgcttcgga gaacaacacc ggtactagca
    80641 ggttgccgtc cagcgcctga ataatcagat acaccgcgaa gcagctccag aattcagtac
    80701 ctgcgccaaa ctggaatagc gccacgccaa ccaccggaat ggtcaccaca aatgcgccaa
    80761 tgtacggaat aagaaccgag aagccgacca gcactgccag cagcagcgaa tagttcagcc
    80821 cgaagagcaa gaaccccagc caggtggcga tccccaccac gatcatctcc agcactttgc
    80881 cgcggatata gttggtgatt tgttgattca tctccttcca cacctgtcct gccagcccac
    80941 ggttacgcgg cagcacccgg cgaacggcgt tcagcatctg ctctttgtct ttcagcagga
    81001 agaagaccat caatggcacc agcaccagat agacggctat ggtcagcaaa ccgaccagcg
    81061 aggcgaggga aattttcacc accgaatcgc ccatggtcag catccgactg cgcatatttt
    81121 cggccattgc atcaataatg cccgcgtcca ttaacaccgg atagcggcgc ggcaacgtgg
    81181 cggcaaagtc agaaagctta ttgagcatcc ccggcatatc gcggattaag tagatgccct
    81241 gttgccaggc gataggcagt accacgaacg ccatcagtag caatataccg acaaaaacca
    81301 ccaatacaat cgacgtcgcc cagcggcggg agcagccaat agattgcagg cgcacggttg
    81361 gccattccag caaataagcc agcacaatag ccaccagcaa cggagcaagc aggccactaa
    81421 agaaaaagat aatgccaaat ccggcaacta aaataaccag caaggcaatc gcttccgggt
    81481 cgctaaaacg gcggcgatac cattgcatca acatttcgag cataaagacc ttccctgaac
    81541 ctcaagagcg ggattgcgat ccgcaattgt atcgaaatgt cacaaaaaag acttcgcttt
    81601 ttatgacgga ttcaggaaac tgaaaagtca tttgagtggg ctaatcttcg ccgttacact
    81661 caaaggcggc gaggtgggaa cgatatttca cagtatcggt caaatgacta tcttcagaaa
    81721 tacaggatag aggttatgtt caggcagttg aaaaaaaacc tggttgcaac cctcattgct
    81781 gctatgacca ttggtcaggt agccccggcg tttgccgaca gcgcagacac cttgccggat
    81841 atgggaacct ccgcaggaag cacgctttcc attggtcagg aaatgcagat gggcgactat
    81901 tatgtccgcc agctacgcgg cagcgcgccg ttaattaatg acccgctgtt aacgcaatat
    81961 attaattcgc tggggatgcg tctggtttcg catgccaatt cggttaagac accgtttcat
    82021 ttttttctga tcaacaacga cgaaattaac gcctttgctt tctttggcgg caacgtggtg
    82081 ctgcactctg ccctgttccg ttattccgat aacgaaagtc aactggcttc agttatggcg
    82141 cacgaaatct cccacgtcac ccaacgtcac ctggcgcgag cgatggaaga tcaacaacgc
    82201 agcgcgccgc tgacctgggt cggcgcgtta ggttctattt tactggcgat ggccagtccg
    82261 caggcgggga tggcggcgct gaccggtaca ctggcgggaa cgcgccaggg gatgatcagt
    82321 ttcacccagc aaaatgaaca ggaagcggac cgcattggta ttcaggtgct gcaacgctcg
    82381 ggattcgatc cacaggcgat gccgaccttc ctggaaaaat tgctcgatca ggcgcgttac
    82441 tcctcgcgcc cgccggaaat tttattgact cacccgttgc cggaaagtcg tctggcagat
    82501 gcccgcaacc gtgctaatca gatgcgcccg atggtggtgc agtcgtcgga agatttctat
    82561 ctggcgaaag cgcgcacact ggggatgtat aattccggac gtaaccagct caccagtgat
    82621 ttgctggatg aatgggcgaa aggaaacgtt cgtcagcaac gagcggcgca atatggtcgt
    82681 gctttacagg cgatggaagc caataaatac gacgaggcgc gtaaaacgct gcaaccgtta
    82741 ctggcggcag aacctggcaa cgcatggtat ctcgatctgg ctaccgatat cgatcttggg
    82801 caaaacaaag ccaatgaggc aatcaatcgc ctgaaaaatg cccgcgattt acgcaccaat
    82861 ccggtgttgc agctcaacct ggcgaacgct tatctgcaag gcggtcaacc acaagaagcg
    82921 gccaatattc tgaatcgcta cacctttaat aataaagatg acagcaacgg ctgggatttg
    82981 ctggcacagg cggaagccgc gctaaataac cgcgatcagg agctggctgc gcgagcagaa
    83041 ggttatgcgc tcgccggacg actcgatcag gccatttcgc tgttgagtag cgccagttcg
    83101 caggtgaaat taggcagcct gcaacaagcg cgttacgatg cgcgcatcga ccagttgcgc
    83161 cagctgcaga aacgctttaa gccttatacc aagatgtaat tcgcattaaa ggaagaacaa
    83221 tgaccaaaca ggtaaaaatc taccataacc cacgctgttc aaagagccgg gaaacgctga
    83281 acctgctgaa agaaaacggc gtggagccag aagtggtgct ctaccttgag acacccgccg
    83341 atgcggcaac gctgcgcgat ttgctgaaaa tgctggggat gaatagcgcc cgtgaattga
    83401 tgcgccagaa agaggatctt tataaagaac taaacctggc tgacagttcg cttagcgaag
    83461 aggcgctgat tcaggcaatg gtggataatc cgaagctgat ggagcgcccg attatggtcg
    83521 cgaatggcaa agcgcggatt ggtcgtccac cggagcaggt actggaaatc gtgggttaag
    83581 atgtgctcca tcgctagttg aagcacattg ccggatgcga cgcttgccac gtcttatccg
    83641 gcctacgaat actatggatt aacttcgcgt aggtcaggcg ttcgcgccgc atccgacaat
    83701 aaacacctta tctacaactt cagaatttct ttcacaaacg gaatggtcag cttacgttgc
    83761 gcggtaatcg acgcacgatc caactgatcc aacgtcataa atagcgtgcg catttctctg
    83821 tcgagccgct tcagcaagaa acgccccaca tcttccggca gttcaaaacc acgcaaacgc
    83881 gcgcgtaact gtagcgcctg caacttatct tcatcagaaa gtggctgcaa tttgtagatc
    83941 tgcccccagt cgagacgcga cgcgagatcc ggtaatccca gattcaactg ccgcggtgga
    84001 cgatcgccgg tgatcaacaa ccgtgttttg cccgattcca gaattcgatt gtagagatcg
    84061 aaaatcgcca tctcccacaa ctcatcgcct gcaatacact caatgttgtc gatacagacc
    84121 agcgacaaat gctccatacc gtcgagcact tccggaacaa accaggtgcg tttatccagc
    84181 gggacatagc ccaccgcatc gccacgctgc gacaattccg cgcaagccgc gtgcagcaga
    84241 tggctgcgcc ccgcgccttc gcgtgcccag agatagatgt aaccgctatg ttcctgacgc
    84301 agcacgtttt gcagcgcggc cagtaaagag gagttatccc ccggccagaa acttgcaaag
    84361 gtttcgtcgt caggaagata aagtggcaaa gagagctgtg ccggtgtgtt cagagatacc
    84421 tcaaccagga tttcacaaaa tcgcgagaag tttaccacag aatcccatga tgtttgaacc
    84481 gggcagcaac actgcccggt cggtacatta tttgtctgtt atatccgcgt cttctgcgtc
    84541 cagcaccact tcttcagggc gcagcacact gatcagtttg aaaatcaaac ttaagccgat
    84601 accgacgatg gtcgccagcg ccataccttt taactccgcc gcgccgatgt ttaccttcgc
    84661 gccactgacg ccgatgatca aaatcacgga agtcaggatc aggttctgtg ctttgttgta
    84721 atccactttc gattcgatca aaacacgaat accggaagca ccaatgacac cataaagcag
    84781 cagcgaaacg ccgcccatca ccggcaatgg gatcatctgg atagcggcag ccagtttacc
    84841 gacgcaggaa agcaggatag cgaaaatcgc cgccccgccg ataacccagg tactgtaaac
    84901 acgggtgatc gccatcacgc caatgttttc tccgtaagtg gtatttggcg tagagccaaa
    84961 gaagccggag atcacggtcg acaggccgtt agcaaacatc gaacggtgca gacctggatc
    85021 gcgcagcaga tcttttttga cgatattagc cgttactacc aggtgcccta cgtgttcggc
    85081 aataaccact aacgccgctg gcagaatagt cagaatggca aaccactcga agcgcggcgt
    85141 atagagggtt ggcagtgcaa accagtgagc attaataatc ggcgtggtat cgacaattcc
    85201 cattgcgaaa gagagcgcgt accccaccag cacgccaatt aaaatcggga taattgccag
    85261 gaaaccacga aacagcacgg aacctaaaac cgtgaccgcc agggtggtaa tagagatgat
    85321 gatggttttg gagtttggcg tttgcccttc agccgggagt aaacccgcca taccggcagc
    85381 tacgcccgcc agctccagac cgatgacggc aacgattgcg cccattgccg caggtggaaa
    85441 cagcacgtcc aaccagccgg tccccgcttt cttcacgata aaagaaacca ggcagaacag
    85501 cacgccgcac ataataaagc cgcccagcgc gacttcatac cctaacggca acagtaacaa
    85561 taccggtgaa ataaaggcaa agctggaacc aagataagcc ggaattttcc ctttacagat
    85621 gaagagatac agcagcgttc caataccgtt aaataacagt acagtcgccg ggttaatatg
    85681 aaataagacg ggcaccagga cggttgcacc aaacatggcg aacaaatgtt gcaaactaag
    85741 cgggattgtc tgtaaaagtg gcggtctttc actcaccccg atagcacggc gcgtcatagt
    85801 attatcctct gtattatgtg ttataggcgc tttactcaaa aaaaagccga ctcttaaagt
    85861 cggctttaat tatttttatt ctttatttcg taccaaagat tttgtcaccg gcatcgccga
    85921 ggcccggaat aatgtatccg tgctcgttca gtccctggtc aatcgatgca gtatacagtt
    85981 cgacgtccgg gtgcgctttt tccagcgcag cgataccttc tggcgcagcg accagcacca
    86041 gaactttgat gctgctgcag cccgcttttt tcagcaggtc gatggtcgcg ataacggaac
    86101 caccggttgc cagcattggg tcaacgatca gcgccatacg ctcatcgatg ttagaaacca
    86161 gtttctggaa gtacggtacc ggctccagcg tttcttcatt acggtacata ccgacaacgc
    86221 tgatgcgcgc gctcggaacg ttttccagca caccgtccat cataccaaga cccgcacgca
    86281 gaattggcac aacggtaatt ttcttacctt tgatctggtc gatttctacc gggccgttcc
    86341 agccttcgat agttactttt tccgtttcga ggtcggcggt cgcttcgtaa gtcagcaggc
    86401 tacccacttc ggaagcgagt tcgcgaaagc gcttggtgct gatatcttgc tcacgcatca
    86461 gtcccagctt gtgtttgacg agtgggtgtt tgacttccac gatcttcata ctctttctcc
    86521 tttgaggggc agccacaaaa aaatcgacgg attatacctc ctttcttcag ggcggcaata
    86581 ttattttcgt tgactttagt caaaatgata acagtttgag ataaagttat tttatattca
    86641 aatggttatg aaagaagatt attccatccg aaaactaacc tttgccctgg cacaagtctt
    86701 ctttcgccgc gcgcccgggg aaaagacgtg caaaaaggtt gtgtaaaaca gtctcgcaaa
    86761 cgtttgcttt ccctgttaga attgcgccga attttatttt tctaccgcaa gtaacgcgtg
    86821 gggacccaag cagtgaccga taaaacctct cttagctaca aagatgccgg tgttgatatt
    86881 gacgcgggta atgctctggt tggaagaatc aaaggcgtag tgaagaaaac gcgtcgtccg
    86941 gaagtgatgg gcggtctggg cggcttcggt gcgctgtgtg cattgccgca aaaatatcgt
    87001 gaacccgtgc tggtttccgg cactgacggc gtaggtacca agctgcgtct ggcaatggac
    87061 ttaaaacgtc acgacaccat tggtattgat ctggtcgcca tgtgcgttaa tgacctggtg
    87121 gtgcaaggtg cggaaccgct gtttttcctc gactattacg caaccggaaa actggatgtt
    87181 gataccgctt cagcggtgat cagcggcatc gcggaaggtt gtctgcaatc gggctgttct
    87241 ctggtgggtg gcgaaacggc agaaatgccg gggatgtatc acggtgaaga ttacgatgtc
    87301 gcgggtttct gcgtgggcgt ggtagaaaaa tcagaaatca tcgacggctc taaagtcagc
    87361 gacggcgatg tgctgattgc actcggttcc agcggtccgc actcgaacgg ctattcgctg
    87421 gtgcgcaaaa ttcttgaagt cagcggttgt gatccgcaaa ccaccgaact tgatggtaag
    87481 ccattagccg atcatctgct ggcaccgacc cgcatttacg tgaagtcagt gctggagttg
    87541 attgaaaagg tcgatgtgca tgccattgcg cacctgaccg gcggcggctt ctgggaaaac
    87601 attccgcgcg tattgccaga taatacccag gcagtgattg atgaatcttc ctggcagtgg
    87661 ccggaagtgt tcaactggct gcaaacggca ggtaacgttg agcgccatga aatgtatcgc
    87721 accttcaact gcggcgtcgg gatgattatc gccctgcctg ctccggaagt ggacaaagcc
    87781 ctcgccctgc taaatgccaa cggtgaaaac gcgtggaaaa tcggtatcat caaagcctct
    87841 gattccgaac aacgcgtggt tatcgaataa tgaatattgt ggtgcttatt tccggcaacg
    87901 gaagtaattt acaggcaatt attgacgcct gtaaaaccaa caaaattaaa ggcaccgtac
    87961 gggcagtttt cagcaataag gccgacgcgt tcggccttga acgcgcccgc caggcgggta
    88021 ttgcaacgca tacgctcatc gccagcgcgt ttgacagtcg tgaagcctat gaccgggagt
    88081 tgattcatga aatcgacatg tacgcacccg atgtggtcgt gctggctggt tttatgcgca
    88141 ttctcagccc ggcgtttgtc tcccactatg ccgggcgttt gctgaacatt cacccttctc
    88201 tgctgccgaa atatcccgga ttacacaccc atcgtcaggc gctggaaaat ggcgatgaag
    88261 agcacggtac atcggtgcat ttcgtcaccg atgaactgga cggtggcccg gttattttac
    88321 aggcgaaagt cccggtattt gctggtgata cggaagatga catcaccgcc cgcgtgcaaa
    88381 cccaggaaca cgctatttat ccactggtga ttagctggtt tgccgatggt cgtctgaaaa
    88441 tgcacgaaaa cgccgcgtgg ctggatggtc aacgtctgcc gccgcagggc tacgctgccg
    88501 acgagtaatg cccccgtagt taaagcgcca gctctgccgc tggcgttttt caattcacct
    88561 gtaaatcgca agctccagca gtttttttcc cccttttctg gcatagttgg acatctgcca
    88621 atattgctcg ccataatatc caggcagtgt cccgtgaata aaacggagta aaagtggtaa
    88681 tgggtcagga aaagctatac atcgaaaaag agctcagttg gttatcgttc aatgaacgcg
    88741 tgcttcagga agcggcggac aaatctaacc cgctgattga aaggatgcgt ttcctgggga
    88801 tctattccaa taaccttgat gagttctata aagtccgctt cgctgaactg aagcgacgca
    88861 tcattattag cgaagaacaa ggctccaacg ctcattcccg ccatttactg ggcaaaattc
    88921 agtcccgggt gctgaaagcc gatcaggaat tcgacggcct ctacaacgag ctattgctgg
    88981 agatggcgcg caaccagatc ttcctgatta atgaacgcca gctctccgtc aatcaacaaa
    89041 actggctgcg tcattatttt aagcagtatc tgcgtcagca cattacgccg attttaatca
    89101 atcctgacac tgacttagtg cagttcctga aagatgatta cacctatctg gcggtggaaa
    89161 ttatccgtag cgatagcatc cgttacgcgc tgctggagat cccatcagat aaagtgccgc
    89221 gctttgtgaa tttaccgcca gaagcgccgc gtcgacgcaa gccgatgatt cttctggata
    89281 acattctgcg ttactgcctt gatgatattt tcaaaggctt ctttgattat gacgcgctga
    89341 atgcctattc aatgaagatg acccgcgatg ccgaatacga tttagtgcat gagatggaag
    89401 ccagcctgat ggagttgatg tcttccagtc tcaagcagcg tttaactgct gagccggtgc
    89461 gttttgttta tcagcgcgat atgcccaatg cgctggttga agtgttacgc gaaaaactga
    89521 ctatttcccg ctacgactcc atcgtccccg gcggtcgtta tcataatttt aaagacttta
    89581 ttaatttccc caatgtcggc aaagccaatc tggtgaacaa accactgccg cgtttacgcc
    89641 atatttggtt tgataaagcc cagttccgca atggttttga tgccattcgc gaacgcgatg
    89701 tgttgctcta ttatccttat cacacctttg agcatgtgct ggaactgctg cgtcaggctt
    89761 cgttcgaccc gagcgtactg gcgattaaaa ttaacattta ccgcgtggcg aaagattcac
    89821 gcatcatcga ctcgatgatc cacgccgcac ataacggtaa gaaagtcact gtggtggttg
    89881 agttacaggc gcgtttcgac gaagaagcca acattcactg ggcgaagcgc ctgaccgaag
    89941 caggcgtgca cgttatcttc tctgcgccgg ggctgaaaat tcacgccaaa ctgttcctga
    90001 tttcacgtaa agaaaacggt gaagtggtcc gttacgcaca catcgggacc gggaacttta
    90061 acgaaaaaac cgcgcgtctt tatactgact attcgttgct gaccgcagat gcgcgtatca
    90121 ccaacgaagt acggcgggta tttaacttta ttgaaaaccc ataccgcccg gtgacatttg
    90181 attatttaat ggtgtcaccg caaaactctc gccgtctgtt atatgaaatg gtagaccgcg
    90241 aaatcgccaa cgcgcagcaa gggctgccca gtggtatcac cctgaagcta aataaccttg
    90301 tcgataaagg cctggttgat cgtctgtatg cggcctccag ctccggcgta ccggttaatc
    90361 tgctggttcg cggaatgtgt tcgctgatcc ccaatctgga aggcattagc gacaacattc
    90421 gtgccatcag tattgttgac cgttaccttg aacatgaccg ggtttatatt tttgaaaatg
    90481 gcggcgataa aaaggtctac ctttcttccg ccgactggat gacgcgcaat attgattatc
    90541 gtattgaagt ggcgacaccg ctgctcgatc cgcgcctgaa gcagcgggtg ctggacatca
    90601 tcgacatatt gttcagcgat acggtcaaag cacgttatat cgataaagaa ctcagtaatc
    90661 gctacgttcc ccgcggcaat cgccgcaaag tacgggcgca gttggcgatt tacgactaca
    90721 tcaaatcact cgaacaacct gaataaccct atgccaatac acgataaatc ccctcgtccg
    90781 caggagtttg ctgcggtcga tcttggttca aacagttttc acatggtcat agcccgtgtg
    90841 gtagatggtg ccatgcagat tattggccgc ctgaaacagc gggtgcatct ggcggacggc
    90901 ctggggccag ataatatgtt gagtgaagag gcaatgacgc gcggtttgaa ctgtctgtcg
    90961 ctgtttgccg aacggctaca agggttttct cctgccagcg tctgtatagt cggcacccat
    91021 acgctgcgtc aggcgctgaa cgccactgac tttctgaaac gcgcggaaaa ggtcattccc
    91081 tacccgattg aaattatttc cggtaatgaa gaagcccgtc tgatttttat gggcgtggaa
    91141 catacccaac cggaaaaagg tcgcaaactg gttattgata ttggcggcgg atctacggaa
    91201 ctggtgattg gtgaaaattt cgaacctatt ctcgttgaaa gccgccggat gggttgtgtc
    91261 agctttgccc agctttattt tcctggcggg gtcatcaata aagagaattt tcagcgcgct
    91321 cgcatggcgg cagcacaaaa actggaaact ttaacctggc aattccgtat tcagggctgg
    91381 aacgttgcaa tgggcgcttc cggtaccata aaagccgccc atgaagtgtt aatggaaatg
    91441 ggcgagaaag acgggataat taccccggaa cgtctggaaa aactggtaaa agaaatttta
    91501 cgtcaccgta atttcgcatc gctgagttta ccgggtcttt ccgaagagcg gaaaacagtc
    91561 ttcgttcctg gactggcgat tttatgtggt gtgtttgatg ctttagccat ccgtgaactg
    91621 cgcctttctg acggggcgtt acgcgaaggc gtactgtatg aaatggaagg acgtttccgt
    91681 catcaggatg tgcgtagtcg caccgccagc agcctcgcca accagtatca catagacagc
    91741 gaacaggccc gacgagtgct ggataccact atgcaaatgt acgaacagtg gcgggaacag
    91801 caaccgaagc tggcgcatcc gcaactggag gcgctactgc gatgggccgc catgctgcat
    91861 gaggtcgggt tgaatatcaa ccacagcggt ttgcatcgcc actccgctta tattctgcaa
    91921 aacagtgact tgccgggttt taatcaggaa cagcagctga tgatggcgac actggtgcgc
    91981 tatcaccgta aagcgattaa gctcgacgat ctgccgcgct ttaccttgtt taagaagaaa
    92041 cagttcctgc cactgataca gctattgcgc cttggcgtat tactaaacaa tcaacgtcag
    92101 gcaaccacca caccgccaac attgacactg attactgatg acagtcactg gacactgcgt
    92161 ttcccgcatg actggtttag tcagaatgcg ctggtactgc ttgatctgga aaaggagcaa
    92221 gaatactggg aaggcgtggc tggctggcgg ttgaaaattg aagaagaaag taccccagaa
    92281 atcgccgctt aatgctttgc gggccgacga gaaatgtcgg cccgcattat tcaggcactt
    92341 tcgcgaatgg gttcgatttc attcagcgta tcaattaacg gttgcggctt accaataaga
    92401 taaccctgca tataatcgat ccccaaagag agcaccgcct cgcggatctc ttcgttttca
    92461 acgtactctg ccactacccg cattttcttc attcgcgcca gatggcaaat cgacgccaca
    92521 atctgataat ccagactatt tgacacaata ttgcggataa agctgccgtc aattttaagc
    92581 agatcggcat tcacattttt aagccgtgca tagctggcgt agccggtgcc gaaatcatca
    92641 atcgcaatct ggcagcctaa ttcctgaaga tgctgcaagg tgatttgcgc ctgcttaaca
    92701 ttggtcagag cattactttc ggtgacttca aaaataagtt gccacgcttc aatctgatat
    92761 ttagccagca actgactgac ttcaacagga aaacgagcct gacataccga ggttggagac
    92821 agattaatag caaaacggtg agcgggcatc ttcgctctgt tttcagccat aaattgcagc
    92881 gtatgctcaa tgacccacat gtcgatactc gacgataaac caaattcgtg cgcgaccggc
    92941 aagaagctac cggggccgat cagttcatca ttctcacctt tcatgcgcag aagaatttca
    93001 tggtaaacat caccacgcat accggtaatc ggctgggcca tcaggaaaaa atggttgtgt
    93061 tccagcgcct gctgtagtcg attcatcatc gcgactttat ctttcaattc gcgttgcaaa
    93121 tacattgccc cgcaacgctg catattttcc ggggcgttgg tcacgatgga aagttcggcg
    93181 accgtattta gctctcccag cagcaggtag atatgattca ctggcgagcg cacatagcag
    93241 taactgacgc caatctgcgg ttgcatcggc atcccatccc aaaagaaacg aaattgcttg
    93301 agatgactat ccagtgcggt aatgcgctcc tggtgcgatt ctgtattcag tcgcagcgcg
    93361 agatcgttac ccgaaagctg ataaacatct tcacccggtt ccagcaaggg tgacagccag
    93421 tgagaaagtt tttgctt
//
LOCUS       DAJTYM010000003        73229 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTYM010000003 DAJTYM010000000
VERSION     DAJTYM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 73229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..575)
                     /locus_tag="OO575_000198"
     CDS             complement(<1..575)
                     /locus_tag="OO575_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR6663302.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILEL"
     gene            complement(646..2760)
                     /gene="fusA"
                     /locus_tag="OO575_000199"
     CDS             complement(646..2760)
                     /gene="fusA"
                     /locus_tag="OO575_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6663303.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2857..3327)
                     /gene="rpsG"
                     /locus_tag="OO575_000200"
     CDS             complement(2857..3327)
                     /gene="rpsG"
                     /locus_tag="OO575_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6663304.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3424..3798)
                     /gene="rpsL"
                     /locus_tag="OO575_000201"
     CDS             complement(3424..3798)
                     /gene="rpsL"
                     /locus_tag="OO575_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6663305.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3924..4211)
                     /gene="tusB"
                     /locus_tag="OO575_000202"
     CDS             complement(3924..4211)
                     /gene="tusB"
                     /locus_tag="OO575_000202"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6663306.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4219..4578)
                     /gene="tusC"
                     /locus_tag="OO575_000203"
     CDS             complement(4219..4578)
                     /gene="tusC"
                     /locus_tag="OO575_000203"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6663307.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4578..4964)
                     /gene="tusD"
                     /locus_tag="OO575_000204"
     CDS             complement(4578..4964)
                     /gene="tusD"
                     /locus_tag="OO575_000204"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6663308.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4964..5686)
                     /locus_tag="OO575_000205"
     CDS             complement(4964..5686)
                     /locus_tag="OO575_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6663309.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5853..6665)
                     /gene="fkpA"
                     /locus_tag="OO575_000206"
     CDS             complement(5853..6665)
                     /gene="fkpA"
                     /locus_tag="OO575_000206"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6663310.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6886..7104
                     /locus_tag="OO575_000207"
     CDS             6886..7104
                     /locus_tag="OO575_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6663311.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7153..7743)
                     /gene="slyD"
                     /locus_tag="OO575_000208"
     CDS             complement(7153..7743)
                     /gene="slyD"
                     /locus_tag="OO575_000208"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6663312.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7838..8038)
                     /locus_tag="OO575_000209"
     CDS             complement(7838..8038)
                     /locus_tag="OO575_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6663313.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(8048..9785)
                     /gene="kefB"
                     /locus_tag="OO575_000210"
                     /pseudo
     CDS             complement(8048..9785)
                     /gene="kefB"
                     /locus_tag="OO575_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9785..10336)
                     /gene="kefG"
                     /locus_tag="OO575_000211"
     CDS             complement(9785..10336)
                     /gene="kefG"
                     /locus_tag="OO575_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6663314.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10467..12380
                     /locus_tag="OO575_000212"
     CDS             10467..12380
                     /locus_tag="OO575_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6663315.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12380..13402
                     /locus_tag="OO575_000213"
     CDS             12380..13402
                     /locus_tag="OO575_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6663316.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13396..13614
                     /locus_tag="OO575_000214"
     CDS             13396..13614
                     /locus_tag="OO575_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6663317.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13668..14537
                     /locus_tag="OO575_000215"
     CDS             13668..14537
                     /locus_tag="OO575_000215"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6663318.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14592..14996)
                     /locus_tag="OO575_000216"
     CDS             complement(14592..14996)
                     /locus_tag="OO575_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6663319.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15298..15930
                     /gene="crp"
                     /locus_tag="OO575_000217"
     CDS             15298..15930
                     /gene="crp"
                     /locus_tag="OO575_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6663320.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15981..18071
                     /locus_tag="OO575_000218"
     CDS             15981..18071
                     /locus_tag="OO575_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6663321.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18138..19358)
                     /gene="argD/dapC"
                     /locus_tag="OO575_000219"
     CDS             complement(18138..19358)
                     /gene="argD/dapC"
                     /locus_tag="OO575_000219"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6663322.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19444..20007)
                     /gene="pabA"
                     /locus_tag="OO575_000220"
     CDS             complement(19444..20007)
                     /gene="pabA"
                     /locus_tag="OO575_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6663323.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(20039..20641)
                     /locus_tag="OO575_000221"
     CDS             complement(20039..20641)
                     /locus_tag="OO575_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6663324.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20631..20798)
                     /locus_tag="OO575_000222"
     CDS             complement(20631..20798)
                     /locus_tag="OO575_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6663325.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20903..21484)
                     /gene="ppiA"
                     /locus_tag="OO575_000223"
                     /gene_synonym="rotA"
     CDS             complement(20903..21484)
                     /gene="ppiA"
                     /locus_tag="OO575_000223"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6663326.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21755..22936
                     /gene="tsgA"
                     /locus_tag="OO575_000224"
     CDS             21755..22936
                     /gene="tsgA"
                     /locus_tag="OO575_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6663327.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23198..25741
                     /gene="nirB"
                     /locus_tag="OO575_000225"
     CDS             23198..25741
                     /gene="nirB"
                     /locus_tag="OO575_000225"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6663328.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25738..26064
                     /gene="nirD"
                     /locus_tag="OO575_000226"
     CDS             25738..26064
                     /gene="nirD"
                     /locus_tag="OO575_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6663329.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26190..26996
                     /gene="nirC"
                     /locus_tag="OO575_000227"
     CDS             26190..26996
                     /gene="nirC"
                     /locus_tag="OO575_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6663330.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27015..28388
                     /gene="cobA"
                     /locus_tag="OO575_000228"
     CDS             27015..28388
                     /gene="cobA"
                     /locus_tag="OO575_000228"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6663331.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28635..28802
                     /locus_tag="OO575_000229"
     CDS             28635..28802
                     /locus_tag="OO575_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6663332.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29097..30434
                     /gene="frlA"
                     /locus_tag="OO575_000230"
                     /pseudo
     CDS             29097..30434
                     /gene="frlA"
                     /locus_tag="OO575_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30455..31477
                     /gene="frlB"
                     /locus_tag="OO575_000231"
     CDS             30455..31477
                     /gene="frlB"
                     /locus_tag="OO575_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6663333.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31527..32357
                     /gene="frlC"
                     /locus_tag="OO575_000232"
     CDS             31527..32357
                     /gene="frlC"
                     /locus_tag="OO575_000232"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6663334.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32354..33139
                     /gene="frlD"
                     /locus_tag="OO575_000233"
     CDS             32354..33139
                     /gene="frlD"
                     /locus_tag="OO575_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6663335.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33239..33970
                     /gene="phoB"
                     /locus_tag="OO575_000234"
     CDS             33239..33970
                     /gene="phoB"
                     /locus_tag="OO575_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6663336.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34130..35215)
                     /locus_tag="OO575_000235"
     CDS             complement(34130..35215)
                     /locus_tag="OO575_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6663337.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35227..36531)
                     /locus_tag="OO575_000236"
     CDS             complement(35227..36531)
                     /locus_tag="OO575_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6663338.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36543..36896)
                     /locus_tag="OO575_000237"
     CDS             complement(36543..36896)
                     /locus_tag="OO575_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6663339.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36907..37785)
                     /locus_tag="OO575_000238"
     CDS             complement(36907..37785)
                     /locus_tag="OO575_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6663340.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRITVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37782..39008)
                     /locus_tag="OO575_000239"
     CDS             complement(37782..39008)
                     /locus_tag="OO575_000239"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6663341.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(39009..40172)
                     /locus_tag="OO575_000240"
     CDS             complement(39009..40172)
                     /locus_tag="OO575_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6663342.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(40256..40618)
                     /locus_tag="OO575_000241"
     CDS             complement(40256..40618)
                     /locus_tag="OO575_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6663343.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40635..41540)
                     /locus_tag="OO575_000242"
     CDS             complement(40635..41540)
                     /locus_tag="OO575_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663344.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41810..42814)
                     /gene="trpS"
                     /locus_tag="OO575_000243"
     CDS             complement(41810..42814)
                     /gene="trpS"
                     /locus_tag="OO575_000243"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6663345.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42807..43565)
                     /gene="gph"
                     /locus_tag="OO575_000244"
     CDS             complement(42807..43565)
                     /gene="gph"
                     /locus_tag="OO575_000244"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6663346.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43558..44235)
                     /gene="rpe"
                     /locus_tag="OO575_000245"
     CDS             complement(43558..44235)
                     /gene="rpe"
                     /locus_tag="OO575_000245"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6663347.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44253..45089)
                     /gene="dam"
                     /locus_tag="OO575_000246"
     CDS             complement(44253..45089)
                     /gene="dam"
                     /locus_tag="OO575_000246"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6663348.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45196..46488)
                     /gene="damX"
                     /locus_tag="OO575_000247"
     CDS             complement(45196..46488)
                     /gene="damX"
                     /locus_tag="OO575_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6663349.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46580..47668)
                     /gene="aroB"
                     /locus_tag="OO575_000248"
     CDS             complement(46580..47668)
                     /gene="aroB"
                     /locus_tag="OO575_000248"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6663350.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47725..48246)
                     /gene="aroK"
                     /locus_tag="OO575_000249"
     CDS             complement(47725..48246)
                     /gene="aroK"
                     /locus_tag="OO575_000249"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6663351.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48647..49885)
                     /gene="hofQ"
                     /locus_tag="OO575_000250"
     CDS             complement(48647..49885)
                     /gene="hofQ"
                     /locus_tag="OO575_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6663352.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49797..50203)
                     /gene="hofP"
                     /locus_tag="OO575_000251"
                     /pseudo
     CDS             complement(49797..50203)
                     /gene="hofP"
                     /locus_tag="OO575_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(50193..50633)
                     /gene="hofO"
                     /locus_tag="OO575_000252"
     CDS             complement(50193..50633)
                     /gene="hofO"
                     /locus_tag="OO575_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6663353.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50617..51156)
                     /gene="hofN"
                     /locus_tag="OO575_000253"
     CDS             complement(50617..51156)
                     /gene="hofN"
                     /locus_tag="OO575_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6663354.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51156..51935)
                     /gene="hofM"
                     /locus_tag="OO575_000254"
     CDS             complement(51156..51935)
                     /gene="hofM"
                     /locus_tag="OO575_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6663355.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            52055..54607
                     /gene="mrcA"
                     /locus_tag="OO575_000255"
     CDS             52055..54607
                     /gene="mrcA"
                     /locus_tag="OO575_000255"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6663356.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54772..55332)
                     /gene="nudE"
                     /locus_tag="OO575_000256"
     CDS             complement(54772..55332)
                     /gene="nudE"
                     /locus_tag="OO575_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6663357.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55652..57787
                     /gene="igaA"
                     /locus_tag="OO575_000257"
     CDS             55652..57787
                     /gene="igaA"
                     /locus_tag="OO575_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6663358.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57852..58520
                     /gene="yrfG"
                     /locus_tag="OO575_000258"
     CDS             57852..58520
                     /gene="yrfG"
                     /locus_tag="OO575_000258"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6663359.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58531..58932
                     /gene="hslR"
                     /locus_tag="OO575_000259"
     CDS             58531..58932
                     /gene="hslR"
                     /locus_tag="OO575_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6663360.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58957..59826
                     /gene="hslO"
                     /locus_tag="OO575_000260"
     CDS             58957..59826
                     /gene="hslO"
                     /locus_tag="OO575_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6663361.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59970..61721)
                     /locus_tag="OO575_000261"
     CDS             complement(59970..61721)
                     /locus_tag="OO575_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6663362.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            62100..63722
                     /gene="pckA"
                     /locus_tag="OO575_000262"
     CDS             62100..63722
                     /gene="pckA"
                     /locus_tag="OO575_000262"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6663363.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63798..65150)
                     /gene="envZ"
                     /locus_tag="OO575_000263"
     CDS             complement(63798..65150)
                     /gene="envZ"
                     /locus_tag="OO575_000263"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6663364.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(65147..65866)
                     /gene="ompR"
                     /locus_tag="OO575_000264"
     CDS             complement(65147..65866)
                     /gene="ompR"
                     /locus_tag="OO575_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6663365.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            66094..66570
                     /gene="greB"
                     /locus_tag="OO575_000265"
     CDS             66094..66570
                     /gene="greB"
                     /locus_tag="OO575_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6663366.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66667..68988
                     /locus_tag="OO575_000266"
     CDS             66667..68988
                     /locus_tag="OO575_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6663367.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69426..69653
                     /gene="feoA"
                     /locus_tag="OO575_000267"
     CDS             69426..69653
                     /gene="feoA"
                     /locus_tag="OO575_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6663368.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69670..71991
                     /gene="feoB"
                     /locus_tag="OO575_000268"
     CDS             69670..71991
                     /gene="feoB"
                     /locus_tag="OO575_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6663369.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71991..72227
                     /gene="feoC"
                     /locus_tag="OO575_000269"
     CDS             71991..72227
                     /gene="feoC"
                     /locus_tag="OO575_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6663370.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72430..>73229
                     /gene="rpnA"
                     /locus_tag="OO575_000270"
     CDS             72430..>73229
                     /gene="rpnA"
                     /locus_tag="OO575_000270"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6663371.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIML"
BASE COUNT        17639 a        19852 c        18524 g        17214 t
ORIGIN      
        1 agttccagga ttttcgcttc ccactctgcg tcgccttcca gcgctttcag agcagaacca
       61 cgaacgatcg gagtgtcgtc gcccgggaag tcgtactgag acagaagttc acgaacttcc
      121 atttcaacca gttccagcag ctcttcgtca tcaaccatgt cgcatttgtt caggaacacg
      181 atgatgtacg gaacgcctac ctgacgaccc agcaggatgt gctcacgagt ctgcggcatc
      241 gggccgtcag tcgcagcaac taccaggatc gcgccgtcca tctgagcagc accggtgatc
      301 atgtttttaa catagtcggc gtgccccggg cagtctacgt gtgcgtagtg acgggtcggg
      361 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
      421 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      481 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      541 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      601 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      661 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      721 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      781 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      841 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      901 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      961 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
     1021 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
     1081 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
     1141 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
     1201 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
     1261 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
     1321 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1381 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1441 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1501 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1561 cgggtcacac agggtgtcac cagtggttac gtctttcagg ccgatagcag cagcgatgtc
     1621 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1681 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1741 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1801 cagtgcagag aacggctcgt catcacttgc gtgacgttca gccggagtgt ctttaccgtc
     1861 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1921 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1981 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     2041 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     2101 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     2161 tacgccctgg tcagcgtcat tccagttgat agctttcatt ttcaccaggt caacaacacc
     2221 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     2281 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc acgcggtcca ttttgttaac
     2341 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2401 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2461 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2521 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2581 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2641 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2701 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2761 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2821 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2881 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2941 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     3001 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     3061 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     3121 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     3181 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     3241 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     3301 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3361 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3421 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3481 gccggagcag tcaagagcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3541 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3601 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3661 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3721 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3781 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3841 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaaggtgtca
     3901 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3961 ctgacgaaat cagtatagtc aatgggaatg atgtcgttca aaatttgacc agtcaaaccg
     4021 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     4081 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtct
     4141 ccttcactga gcagacgcag cagcgcagca aaatccgtca gccagggtga acgatgtaac
     4201 gtgtgcagca taaacgcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     4261 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggtgtctgcg gatctaaccc
     4321 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gactcaacaa
     4381 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctgacagcag
     4441 ctgaaaaacg ccatcagcaa taaagaagac ggccagatcg tcagttaatg cggaagttgc
     4501 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggtgcag tagaaaaaac
     4561 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4621 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaagc cagccccagc
     4681 cttcctgctt cagtttcatc gacgataccg cggcgtaacg ctgccgctac gcagatattc
     4741 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4801 cttgccggtg aggtcagttg gttagcgtta tagacccctt cccgatagaa aaagacgctg
     4861 cttaacttat ggccttctgc tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4921 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4981 gaaaatcacc gctcttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     5041 ggcggtcagc aacctggttg atcgcatctt taatatcgaa gatccctttc tcgtagagat
     5101 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca ttcacttctt
     5161 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     5221 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     5281 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     5341 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5401 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5461 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5521 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5581 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5641 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5701 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5761 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5821 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5881 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5941 tcaaacacta gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     6001 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     6061 caacccggga taacaccgtc cagacggaaa gaaagcggtt caccacgggt gtaagagttg
     6121 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     6181 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     6241 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     6301 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6361 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6421 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6481 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6541 ttgaacgatg ctttgctgtc tgcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6601 attggtgcat gcagggcaac ggccattgtg gtcgccagta gcgttacttt aaacagtgat
     6661 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6721 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6781 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6841 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6901 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6961 gaactgaacg tcacggtggc cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     7021 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     7081 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     7141 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     7201 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     7261 atcgtggtga tgatcgtgcg cgccgtgaac gtggccatga gccagttctt cttcggtcgc
     7321 ttcgcgaatc gccacaactt caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7381 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7441 gtcagtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7501 tttaggaaca cgttgcacta ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7561 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7621 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7681 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7741 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7801 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7861 tcacttgctc atctttgtga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7921 gtccgcactt aacacattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7981 cctgcgcctg acaggccggg cattttgcgc ccgctataaa acgttttcgg attgccatcc
     8041 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     8101 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacggtat
     8161 cagcatgcat cgggattaac tcccgcagca ttcgcatatc caggcggcga aaatgcagtt
     8221 gcgcccgctg tgcctgatgc ggatgcatgc caagcgtgac cagcgtcttg cgccccagct
     8281 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     8341 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8401 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8461 cggcctctgc acccgcagaa cgtaaaagat cgacctgcgt ggcgtcgccg taataaactt
     8521 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8581 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8641 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8701 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgcc atctggtcgc
     8761 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8821 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8881 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     8941 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9001 cgagcagcaa gcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9061 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9121 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9181 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9241 cgacaaactc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgacc
     9301 ccgccaacaa cggcactaac gccagtgctg ggatcactgc cagatcctga aacagcagaa
     9361 ccgaaaaccc gagctggccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9421 acgccattgc agttgaagac atcgcaaagc caatgccacc gaccaccgcc gcctgccagg
     9481 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9541 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagttt ggagggattc aactcaaggc
     9601 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9661 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc cgccagcaaa tatcccaaca
     9721 cagcgccaat ccccagccgc gaagagaaac agcactcctg cgagtaaaaa atcggaacct
     9781 tccatcagcg gcctcctgga gacagcggat ttgccaacca gtcaccgtag gctctggcgt
     9841 ggctcgccag ctcctttgcg ctttgccgtc tcgcccagta aataatgatg ggacttaacc
     9901 aatgcatccg gcacatgccc gccgccagtt caaaggggcg cagcacatcg ctcatcgggt
     9961 agcgattcag cgcgtcataa cggtaagcac tttccggctc gccggtggta atcacgttac
    10021 gccagtactt tcccgccagt tggtttcctc ccggcccgct ggcaaaacca cgacttaata
    10081 cccggtccag ccactctttc agtagcgccg ggcagctata ggtataaaga ggatgctgaa
    10141 agacaatcac ctcgtgctcg cgcagtaatg cctgctcacg ggggatatca ataaaaaaat
    10201 cgggatagtg cgcgtaaagg tcgtgcacgg taacattgct gagctgcgtg gccggtttaa
    10261 gcagtacccg gtttgccacc gagtcctgag attccggatg ggcatacagc agcaaaactt
    10321 tcgctggctg agacatcatc cccctcccgg tatggttttt gtgtatagtc gctgttttgg
    10381 gctaccattg cgcccggtgc ggcagctcgc ccatacatta cattatcata atgataagtt
    10441 aacatagtct gaacatacgg cgccttatga ttgttttctc ctcgttacaa attcgtcgcg
    10501 gcgtgcgcgt cctgctggat aatgccaccg ccaccatcaa ccccgggcag aaagtcggcc
    10561 tggtgggtaa aaacggctgt ggtaaatcta ccctgctggc attgctgaaa aatgaaatca
    10621 gcgccgacgg cggcagctac acctttccgg gaagctggca actggcgtgg gtgaatcagg
    10681 aaacgccagc gttaccgcaa gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc
    10741 gtcaactgga agcgcagctg cacgacgcca acgaacgtaa cgacgggcac gccattgcga
    10801 ccattcatag caagctggat gctattgacg catggagtat tcgctcccgt gccgccagcc
    10861 tgctgcacgg cctcggtttc agcaatgaac aactggagcg cccggtaagt gatttctccg
    10921 gtggctggcg tatgcgtctt aaccttgccc aggcgctgat ttgccgttca gacttgctgc
    10981 tgctcgacga accgactaac cacctcgatc tcgatgccgt tatctggctg gaaaaatggt
    11041 tgaagagcta tcagggcacg ctgatcctga tctctcacga ccgcgacttc ctcgatccga
    11101 tcgttgataa aattattcat atcgaacaac aaagcatgtt cgagtacacc ggcaactaca
    11161 gttcgtttga agtacagcgc gccacccgtc tggcgcagca acaagcgatg tacgaaagcc
    11221 agcaggaacg cgtggcacat ctgcaaagtt atatcgatcg tttccgtgcc aaagccacca
    11281 aagcgaagca ggcccagagc cgcattaaga tgctggaacg tatggagctg attgcccccg
    11341 cacacgtcga caacccgttc cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt
    11401 tactgaagat ggaaaaggtc agcgcaggct atggcgatcg cattattctc gactcgatta
    11461 aattgaatct ggtccccggc tcgcgcattg gtctgttagg ccgcaacggc gcaggtaaat
    11521 cgacattaat caaactgtta gccggtgaac ttgcgccagt cagcggtgaa attggtctgg
    11581 cgaaagggat caagctcggc tacttcgccc agcatcaact tgaatacctg cgcgccgacg
    11641 aatcacctat tcaacatctg gcacgtttag cgccgcagga gctggaacaa aaactgcgtg
    11701 actacctcgg cggctttggt ttcctgggcg ataaagtaac cgaagaaacg cgccgcttct
    11761 ccggtgggga aaaagcccgc ctggtgctgg cattaattgt ctggcagcgt ccgaatctgc
    11821 tgctgctcga cgaaccgact aaccaccttg acctcgacat gcgtcaggca ctcaccgaag
    11881 cattaatcga gtttgaaggc gcgctggttg tcgtttcgca cgaccgtcat ttgctgcgtt
    11941 ccaccactga cgatctctac ctggttcacg atcgtaaagt cgaaccgttc gacggcgatc
    12001 tggaagatta tcaacagtgg ttgagcgacg tacaaaagca ggaaaaccag accgacgaag
    12061 cgccaaaaga gaacgcgaac agcgcccagg cacgtaaaga tcagaagcgt cgggaagcgg
    12121 agctgcgtgc gcaaacccag ccgctgcgta aagagattgc ccgtctggaa aaagagatgg
    12181 agaagctgaa cgcgcaactg gcgcaggcgg aagagaaact cggcgacagc gaactgtatg
    12241 accagagccg taaagcggag ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg
    12301 gcctggaaga gtgcgaaatg gcgtggctgg aagcccagga gcagcttgag cagatgctgc
    12361 tggaaggcca aagcaactga tggcgcagat aacgacgacc gatgccaatg aatttagcag
    12421 cagtgctgaa ttcaccccta tgcgcggctt tagcaattgt catctacaaa ctatgctgcc
    12481 gcgtttgttt cgtcgtcagg tgaaattcac cccgtactgg cagcggctgg agttgcccga
    12541 cggcgatttt gtcgatctcg cgtggagtga agaccccgca caggcgaaac ataaaccgcg
    12601 tttagtggtg tttcacgggc tggaaggcag tctcaatagc ccttacgccc acggtctggt
    12661 tgaggcggcg caaaagcgcg gctggctggg cgtggtgatg cattttcgcg gatgcagcgg
    12721 tgaaccaaac cgtatgcacc gcatttacca ttcgggcgaa accgaagacg ccagttggtt
    12781 tttacgctgg ctgcaacgcg aatttggtca tgcgccaacg gctgccgtcg gctattcgct
    12841 cggcggtaat atgctggcct gtttgctggc aaaggaaggc aatgatctcc cggttgatgc
    12901 ggcggtgatt gtctctgcgc cgtttatgct ggaagcctgt agctatcata tggaaaaggg
    12961 cttttcccgc gtttatcagc gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa
    13021 gctggcagcc taccccggaa cgctgccgat taatctcgcg cagttaaaat cggtacgtcg
    13081 catccgtgaa tttgacgatc taatcaccgc cagaattcac ggctacgccg acgctatcga
    13141 ctattatcgt cagtgtagcg ccatgccaat gctgaaccgg atcgccaaac cgacgctgat
    13201 tattcacgcc aaagacgatc cgtttatgga tcatcaggtg atcccaaaac cggaaagtct
    13261 cccctcgcag gtggagtatc aactgactga acatggcggt catgttggct ttattggcgg
    13321 tacattactt catccgcaaa tgtggctgga gtcacgcatt cctgactggt taacaacgta
    13381 tctggaggcg aaatcatgtt gattccgtgg caagacctct cccccgaaac gctggaaaat
    13441 ttaattgaaa gctttgtgtt acgtgaaggc accgattatg gtgaacatga gcgtacactt
    13501 gaacagaaag tcgccgacgt caaacgccag ctacagtgcg gagaagcggt gctggtatgg
    13561 tcggagctgc acgaaacggt caatattatg ccgcgcagtc agtttcgcga ataacctgca
    13621 accgtcggcg gacgagataa tagtgatagc taagtcatgg agttactatg tctgccaaac
    13681 atccggtcat tgcggtaaca ggatccagcg gcgcggggac caccaccacc agcctcgcgt
    13741 ttcgtaaaat attcgcgcag ttaaatctgc atgcagctga ggtggaaggc gacagttttc
    13801 accgttacac ccgcccggaa atggacatgg cgatccgcaa agcgcgcgac gccgggcggc
    13861 atatcagcta cttcggcccc gaagcgaacg acttcggcct gctggaacaa accttcattg
    13921 aatacggtca gagcggcaaa gggaaatctc gcaaatatct gcatacctac gacgaagccg
    13981 taccctggaa tcaggtaccg ggaacattca ccccctggca acctttacca gaacccactg
    14041 atgtactgtt ttatgaaggt ttacacggcg gcgtagtcac gccacagcat aacgttgcgc
    14101 agcatgtgga cttactggtc ggcgtagtgc ctatcgttaa ccttgagtgg attcaaaaac
    14161 tgatccgcga caccagcgag cgcgggcact cacgagaagc agtgatggac tcggtagtgc
    14221 gttcaatgga agactatatc aactacatca caccgcagtt ttcccgcacc catcttaact
    14281 tccagcgcgt tcccaccgtc gacacttcaa acccgttcgc ggcaaaaggt atcccgtcgc
    14341 tggatgaaag ctttgtggtg atccattttc gtaatctgga agggatcgat ttcccttggc
    14401 tgctggcgat gttgcaaggc tcattcattt cccacatcaa tacgttagtg gtacctggcg
    14461 gcaaaatggg gctggcaatg gaattaatta tgctgccgct ggtgcaacga ttgatggaag
    14521 gaaagaaaat cgagtaactt tgctattatg ccggataaaa tactatccgg cttcacaacg
    14581 ggatagttaa gtcacgcggc aaccacttca tacgagtgag taatattcac cgctttttcc
    14641 agcatcaacg ccactgaaca atatttctcg gcagagagat caaccgcacg cgcaaccgct
    14701 gcgtctttca ggtcgcgacc ggtgacgata aaatgcagat taatgtgcgt aaacaggcgt
    14761 ggtgcctctt cgcggcgttc agaggtcaat tttacttcac aatcgaccac atcctgacgc
    14821 cctttttgca ggatcgaaac cacatcgatg gcactgcaac cacccgccgc catcaacacc
    14881 atttccatcg gacttggtgc tttatcgcct gagttgccgt ccattaaaat ctggtgacca
    14941 gaggcggatt cgcccagaaa agttaaccct tcgacccact tcactcgcgc ttgcattttt
    15001 gctactccac tgcgtcaatt ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg
    15061 aaggcgacct gggtcatgct gaagcgagac accaggagac acaaagcgaa agctatgcta
    15121 aaacagtcag gatgctacag taatacattg atgtactgca tgtatgcaaa ggacgtcaca
    15181 ttaccgtgca gtacagttga tagccccttc ccaggtagcg ggaagcatat ttcggcaatc
    15241 cagagacagc ggcgttatct ggctctggag aaagcttata acagaggata accgcgcatg
    15301 gtgcttggca aaccgcaaac agacccgact ctcgaatggt tcttgtctca ttgccacatt
    15361 cataagtacc catccaagag cacgcttatt caccagggtg aaaaagcgga aacgctgtac
    15421 tacatcgtta aaggctctgt ggcagtgctg atcaaagacg aagagggtaa agaaatgatc
    15481 ctctcctatc tgaatcaggg tgattttatt ggcgaactgg gcctgtttga agagggccag
    15541 gaacgtagcg catgggtacg tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa
    15601 aaatttcgcc aattgattca ggtaaacccg gacattctga tgcgtctgtc tgcacagatg
    15661 gcgcgtcgtc tgcaagtcac ttcagagaaa gtgggcaacc tggcgttcct cgacgtgacg
    15721 ggccgcattg cacagactct gctgaacctg gcaaaacaac cagatgctat gactcacccg
    15781 gacggtatgc aaatcaaaat tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt
    15841 gaaaccgtgg gacgcattct gaagatgctg gaagatcaga acctgatctc cgcacacggt
    15901 aaaaccatcg tcgtttacgg cactcgttaa tcccgtcgga gtggcgcgtt acctggtagc
    15961 gcgccatttt gtttcccccg atgtggcgca gactgattta tcaccccgat atcaactatg
    16021 cacttcgaca aacgctggtg ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat
    16081 tacgattcgg tctgctcttc tccctcgttc ctgcctgttg caatattgcg ggccttgata
    16141 cgcctcataa acgttttttc aaacacttaa tcattggtgc gtcgctgttt gccacctgta
    16201 gcttactgac acagctgcta ctggtaaaag atgttcccct gccctttttg ctgaccggat
    16261 taacgctggt acttggcgtc actgctgagc tggggccatt acacgcaaaa ttgcttcctg
    16321 catcgctgct cgccgccatt tttaccctca gtctggcggg atacatgccg gtctgggaac
    16381 cattgctcat ctatgcgttg ggcactctct ggtacggatt gtttaactgg ttttggttct
    16441 ggatctggcg cgaacaaccg ctgcgcgagt cactaagtct gctgtaccgt gaactggcag
    16501 attattgtga agccaaatac agcctgctta cccagcacac cgatcctgaa aaagcgctgc
    16561 caccgctgct ggtgcgccag caaaaagcgg tcgatttaat tactcagtgc tatcagcaaa
    16621 tgcatatgct ttccgcgcaa aacaataccg attacaagcg gatgctgcgt attttccagg
    16681 aggcgctgga tttgcaggaa catatttcgg tcagtttgca tcagccggaa gaggtgcaaa
    16741 aactggtcga gcgtagccat gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg
    16801 ctcgcctgcg cgtgctggct gatgacattc tttaccatcg cctgccaacg cgttttacga
    16861 tggaaaagca aattggcgca ctggaaaaaa tcgcccgcca gcatccggat aatccggttg
    16921 ggcaattctg ctactggcat ttcagccgca tcgcccgcgt gctgcgcacc caaaaaccgc
    16981 tctatgcccg tgacttactg gccgataaac agcggcgaat gccgttgctt ccggcactga
    17041 aaagttatct gtcactaaag tctccggcgc tacgcaatgc cggacgactc agtgtgatgt
    17101 taagcgttgc cagcctgatg ggcaccgtgc tgcatctgcc gaagtcgtac tggatcctga
    17161 tgacggtatt gctggtgaca caaaatggct atggcgcaac ccgtctgagg attgtgaatc
    17221 gctccgtggg aaccgtggtc gggttaatca ttgcgggcgt agcgctgcac tttaaaattc
    17281 ccgaaagtta caccctgacg ttgatgctga ttaccaccct cgccagctac ctgatattgc
    17341 gcaaaaacta cggctgggca acggtcggtt ttactattac cgcagtgtat accctgcaac
    17401 tgttgtggct gaacggtgag cagtacatcc ttccacgtct tatcgatacc attattggtt
    17461 gtttgattgc tttcggtggt accgtctggc tgtggccgca gtggcagagc gggttattgc
    17521 gtaaaaacgc ccatgatgct ttagaagcct atcaggaagc gattcgcttg attctgagcg
    17581 aggacccgca accaacgcca ctggcctggc agagaatgcg ggtaaatcag gcgcataaca
    17641 ctctgtataa ctcattgaat caggcgatgc aggaaccggc attcaacagc cattatctgg
    17701 cagatatgaa actgtgggta acgcacagcc agtttattgt tgagcatatt aatgccatga
    17761 ccacgctggc gcgggaacac cgggcattgc cacctgaact ggcacaagag tatttacagt
    17821 cttgtgaaat cgccattcag cgttgtcagc agcgactgga gtatgacgaa ccgggtagtt
    17881 ctggcgatgc caatatcatg gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta
    17941 cgctggagca gcatttacag cgggttattg gccatctgaa caccatgcac accatttcgt
    18001 cgatggcatg gcgtcagcga ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt
    18061 cgaaggcgta atacaggcta aaccatcgga taaggcattc acgccgcatc cgacatattt
    18121 tgcctgatac gtcttattta cgccccaacc accttcgcca ccgcgtgggc gaaacgttgc
    18181 atcccttcat cgatatccgc atcttccacc accagcgacg gtgctaaacg catcacatcc
    18241 ggtccggcat tcagcaccat tacgccaacc tctgcacccg catacaggaa atcacgcgcc
    18301 cgacctttgt agtgtggttt cagctccgcg ccaattaaca gccccatacc gcgaatatcg
    18361 ctaaacacat cgtactgctg atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc
    18421 gcctgaatgc cttccagcac ttcaggggta ttgatgatat caaacgccgc gcccgctact
    18481 gcacaggcca gaggattacc gccgtaggtg gaaccgtgag aaccaggatg aaacgcagaa
    18541 gcaatttccg ccgtggtcag catggcgcta atcgggaagc cgccgcctaa cgctttcgca
    18601 gaagtcagaa tatccggcgt cacaccgtag tacctgtaag caaacaaatc gccagtccgc
    18661 cccatcccgc actgcacttc atcaaacacc aataatgcct gatgttgatc gcacagctcg
    18721 cgcaagccct gcaaaaactc tggcgtcgct gccgtcacac cgccctcgcc ctggatcggc
    18781 tcaaccacca ccgcacaggt gtgatcatcc atcaccgctt tcactgcatg gagatcgtta
    18841 aagggaacgt ggatgatgtc tgccggtttc ggcccaaagc cgtcggaata ttttggctgc
    18901 ccacccaccg aaacggtaaa cagcgagcga ccatgaaaag cgttatggaa ggcaataatt
    18961 ttggttttga acgggctatg acgcacacag gcgtaatggc gtgccagttt aaaggcggtt
    19021 tcgttagctt ccgtgccgga gttcataaac accacgcgtt cggcaaacgt cgcctcaatc
    19081 agtttgcgcc caagacgcag cgccggttca ttggtgaaaa cgttactgat atgccacaga
    19141 gtttcgccct gggtttttaa cgcgttcacc agcgcaggat ggcaatggcc caacgccgta
    19201 actgcaatgc cacccgcgaa atcgacatac tccttgcctt gctgatccca gattcggctg
    19261 ccctgacctt ttaccggaat aaactctgcc ggagcataaa tcggcaggat cacttcatcg
    19321 aaagtcgcgc gtgtaattgc tgtttgttca attgccatct catgatcacc ctgttacgca
    19381 taaataaatg tgaaattata atcacaaaat atgcataaaa aatcactgaa tggcaatcgg
    19441 aaatcagaga tgcaggaaat tagccagcag ttgatgtcct tgttcgctaa gaatactttc
    19501 tggatggaac tgcacacctt ccagatccca ctggcgatgg cgaatcccca taatctcgcg
    19561 ggtttcgctc caggccgtca cgtcaaagca cgctggtaat gagtcaggtt ccaccaccag
    19621 cgaatggtag cgtgtcacgg taagtggatt tgccagcccc cgaaatacgc cctcaccgct
    19681 atgtgtaatc ggcgaggttt tgccgtgcat gacctttgcg gcgcgcacaa ctttgccgcc
    19741 aaatgcctgc gccattgcct gatgaccgag gcagacgcca agaatcggca agcgcccggc
    19801 atagtggcga ataacgtcaa gggagatccc ggcttcatct ggcgtacagg ggccaggtga
    19861 gatgacaatt ttttgtggtt taagggcgtc gatatccgcc agcgtcaacg catcgttgcg
    19921 cttaaccagc acatccgccc ccagttcaca aaagtactgg tagaggttcc aggtaaaaga
    19981 atcgtagtta tctataagca ggatcatggc ggctccggta cgaaagaacc gcgctatttt
    20041 actcagattc cccggcttcg cttaccactt tgctaaatat catctgcagt gcggtgagat
    20101 cccccattgc cccactctga tttgcctgat tccaggcctc tttttcgata ccctgccagc
    20161 tcagttgata tccggcatga atcgccagtt gctcgaagaa gatccgctgt gccagaccac
    20221 ttcccacccg gaagggatgc agcacgttga tttcacaata gtaatgcgcc agccgctcga
    20281 cgaacttcgc tttctccagg ccaaccagat aaccttcttc ctccagatcc tgcatcaggg
    20341 cattgccctc tttttcgata taagcaaagt ggcagaacgg cgtatcgccc tgataaatat
    20401 caacttcacg cagttgccct gcccagtcga aaatatcctg gtacagctgg cgatggatag
    20461 tgcgcaaatg cggtaaaccg cgcaccagcg gaccaagctc aatggtcgca gcacgcagcg
    20521 ccgtcatttc gtaagcggcc tgttccagcc gctgctgctg gcggatgttc agccggttac
    20581 gcatgatatc aaggccagga taaagataca gatcgcgccc ttcgccgaat ttatcgctca
    20641 tagtgcctcc tcagctcttc aaggcgcgct aaagcctctg cggcagtaag agtgactaaa
    20701 ggcatctcga cgccttcaag acggcgactg gcctggaaat tacgattacg ctgaagctcc
    20761 cagagacggg acttttgctt atcggtgagt ttcttcactt gatgcctccc tgaacgtgtc
    20821 catttgccac aagtataagc ggcaaatccg ggttacgccg ggagagcaga agcgcgggca
    20881 agattgcccg cgcgagaaat cattacggca ggactttagc ggaaaggata actaccggtt
    20941 ttgacggcac attctggtac ggaccaacat catgagtcgg cacctgggaa atcttatcgg
    21001 caacgtccat gcctttcacc actttaccaa ataccgcgta accgaaatca cgctgaccat
    21061 ggtcaaggaa ggcgttatcg gcaacgttga taaagaactg gctggtggcg ctgtctttgt
    21121 cagcggtacg cgccatcgcg atggtgccac gcgtgttgcg caggccgtta tcggcttcat
    21181 ttttgattgg cgggtttggt tttttctgct gcatctgctc ggtgaaaccg ccgccctgaa
    21241 tcataaagcc aggaatgacg cggtgaaagg tagtgttgtt ataaaaaccg ctgttcacat
    21301 aatcgacaaa gttttgcaca gacactggcg ctttttgttt atccagctcc agttcgatgt
    21361 taccagctga ggttgtcaac aatacgtgcg ggtccccttt cgctgccatt gctgcgggag
    21421 aaagagcaga aagagcagaa agagcgaaaa cagcagccat cgccgccagg gtcgatttga
    21481 acatgagatt tccttaacaa acagagcaga aaaaaagcaa gtgaagagat tctaaatagc
    21541 cttcaagaac aaggccatcc ctttacctaa ttttacgtat ctgaaatata tgtaacctga
    21601 cgatcacttt ttattccgtg atcaaaatca cctcttaaaa tgcaatttag caaccgattg
    21661 caataaaaca tttaaacaga tcataaaatc acctaaaatc gcccgctcgc ggcatatccc
    21721 gctatgccat tttatttttg aacgcaggcc aatcatgact aacagcaatc gcatcaagct
    21781 cacatggatt agctttctct cctacgcact gactggtgcg ttggttattg tcaccgggat
    21841 ggtgatggga aatatcgccg attatttcaa tctgcctgtt tccagtatga gtaacacctt
    21901 caccttcctc aacgccggca ttttaatctc tatcttcctc aacgcctggc tgatggaaat
    21961 cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg atggtgctgg cggttgccgg
    22021 tttgatgttc agccacagcc tggcgctgtt ctcgacggcg atgttcattc tcggggtggt
    22081 cagcggcatt actatgtcga ttggtacatt cctgataaca caaatgtatg aagggcgtca
    22141 gcgcggttcc cgcctgttat ttactgactc cttcttcagt atggctggga tgattttccc
    22201 aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg tactgggttt atgcctgcat
    22261 cgggctggtg tatgtcgcta tttttattct gaccttcggc tgtgagttcc cggcactggg
    22321 caaacgtgca ccaaaaacgg atgctccggt agagaaagaa aagtggggga tcggcgtact
    22381 gtttctctct gttgcggcgc tgtgctacat cctcggtcag ttaggtttta tctcctgggt
    22441 gcctgagtat gccaaaggcc tgggcatgag cctgaacgac gcgggcacgc tggtgagtaa
    22501 cttctggatg tcatacatgg tcggcatgtg ggcgttcagc tttattcttc gcttctttga
    22561 tttgcaacgc attctgaccg tactggctgg tctggctgcg attctgatgt acgtctttaa
    22621 caccggaaca ccagtacata tggcgtggtc aattctcgct ctgggcttct tctccagcgc
    22681 gatctatacc accatcatca ccctgggttc acagcagacc aaagtaccgt cgccaaaact
    22741 ggttaacttt gtcctgacct gcggaaccat cggtactatg ttgacctttg tggttaccgg
    22801 cacgattgtt gaacatagcg gtccgcaggc ggcactgctt acagcaaacg gtctgtacgc
    22861 tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc cgtcaccgtc agcataacac
    22921 cctgacctct cattaattgc taatgccgga cggcactatc gtcgtccggc cttttcctct
    22981 cttcccccgc tacgtgcatc tatttctata aacccgttca ttttgtccat tttttgcaca
    23041 aacatgaaat gtcagacaat tccgtgactt aagaaaattt atacaaatca gcaatatacc
    23101 ccttaaggag tatataaagg tgaatttgat ttacatcaat aagcggggtt gctgaatcgt
    23161 taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg agcaaagtca gactcgcaat
    23221 tatcggtaac ggtatggtcg gccatcgctt tatcgaagat cttcttgata aatctgatgc
    23281 ggccaacttt gatattaccg ttttctgtga agaaccgcgc atcgcttatg accgcgtaca
    23341 cctctcatct tacttctctc accacaccgc cgaagagctg tcgctggtgc gcgaaggctt
    23401 ctacgagaaa cacggcatca aagttctggt cggcgaacgc gctatcacca tcaaccgtca
    23461 ggagaaggtg attcactcca gcgccggacg taccgttttt tatgacaagc tgatcatggc
    23521 aaccggttcc tacccgtgga tcccaccaat caaaggttct gatactcagg actgctttgt
    23581 ctatcgcacc attgaagacc tcaacgccat tgaatcctgc gcccgtcgca gcaaacgcgg
    23641 tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct ggcgcgctga aaaacttagg
    23701 tattgaaacc cacgttatcg aatttgcccc tatgctgatg gcggaacagc ttgatcagat
    23761 gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc gtgcgcgttc acaccagcaa
    23821 aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt aaaaccatgc gttttgccga
    23881 cggcagcgaa ctggaagtcg actttatcgt cttctctacc ggtatccgtc cgcgcgataa
    23941 gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg gggattgtca ttaatgattc
    24001 ctgccagact tccgatccgg atatctacgc catcggtgaa tgcgcaagct ggaacaaccg
    24061 tgtatttggt ctggtagcac caggctacaa aatggcgcag gtcgccgttg accatattct
    24121 gggtagcgaa aacgcctttg aaggtgctga ccttagcgcc aagctgaaac tgctgggcgt
    24181 agacgtaggt ggtattggtg atgcgcacgg tcgcacgcct ggcgcacgta gctatgttta
    24241 cctcgacgaa agtaaagaga tctacaaacg cctgattgtc agcgaagaca acaaaaccct
    24301 gctcggtgcg gtactggtgg gcgataccag cgactacggc aacctgctgc aactggtgct
    24361 gaacgctatc gagctgccgg aaaacccgga ttctctgatt ctgccggcgc actcgggtag
    24421 cggtaagccg tctatcggtg ttgataaact gccggacagc gcgcaaatct gctcctgctt
    24481 cgacgtcact aaaggtgatc tgattgctgc catcaacaaa ggctgccaca cagttgcggc
    24541 gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc tgtatcccgc tggtcactca
    24601 ggtactgaac gcggaactgg cgaaacaggg catcgaagtt aacaacaacc tgtgcgaaca
    24661 ctttgcttat tcgcgtcagg aactgttcca tttgatccgc gttgaaggca ttaaaacctt
    24721 cgaagaactg ctggcgaaac acggcaaagg ctacggttgt gaagtttgta aaccaaccgt
    24781 cggttcgctg ctggcctcct gctggaacga atacattctg aagccggaac atactccgct
    24841 gcaggattct aacgacaact tcctcgctaa catccagaaa gacggcacct actcggtgat
    24901 cccgcgttct ccgggcggtg aaatcacccc ggaagggctg atggcagtgg gtcgtatcgc
    24961 gcgtgaattt aatctctaca ccaaaatcac cggctcccag cgcctggcga tgtttggcgc
    25021 acagaaagac gatctgccgg aaatctggcg tcagctgatt gaagctggct tcgaaaccgg
    25081 tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc gtgggtagca cctggtgccg
    25141 ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg gaaaaccgct acaaaggcat
    25201 ccgtacgccg cacaaaatga agttcggtgt ctccggctgt acccgtgaat gttcagaagc
    25261 tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc tggaacctgt atgtttgcgg
    25321 taacggcggc atgaaaccgc gtcatgcgga tctgctggca gcggatatcg atcgcgaaac
    25381 gctgatcaaa tatctcgacc gcttcatgat gttctacatc cgtaccgccg acaaactgac
    25441 gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc gattacctga aagcggtgat
    25501 cattgacgac aaactggggc tgaacgctca tctggaagaa gagatggcgc gcctgcgtga
    25561 agcggtagtg tgtgagtgga ctgaaacggt caatacaccg tccgcgcaga ctcgcttcaa
    25621 acacttcatc aacagcgaca agcgtgaccc gaacgtgcag atggtgccag agcgcgaaca
    25681 gcaccgtccg gcaacgccgt atgaacgtat cccggtaact ctggtggagg acaacgcatg
    25741 agccagtgga aagacatctg caaaatcgat gacatcctgc ctgaaaccgg cgtctgcgca
    25801 ctgttaggtg acaagcaggt agcgattttc cgcccgtatc acagcgacca ggtgtttgcg
    25861 atcagcaaca ttgatccgtt cttcgagtcc agcgtgctat cacgcgggct gattgcggaa
    25921 caccagggcg agctgtgggt cgccagcccg ctgaaaaaac agcgttttcg cttaagtgat
    25981 ggcttgtgca tggaagacga acagttttcc gtcaaacatt acgaagcacg agtgaaagac
    26041 ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc gcaatgcctc ccctttttgc
    26101 atggtcctgt aatgaccttc ggtatattgc aggacatttt ttaaactttt tgttttattt
    26161 tttgttttta ttttttaaag gataatcaaa tgtttacaga cactattaat aagtgtgcgg
    26221 ctaatgctgc gcgcattgca cgcctgtcgg caaataaccc gctcggcttt tgggtcagct
    26281 ccgccatggc gggcgcatat gtgggtcttg ggatcatcct gattttcacg ctcggtaatt
    26341 tgctcgaccc gtccgtacgt ccgctggtga tgggcgcgac ctttggtatc gccttaacgc
    26401 tggtgattat cgccggttct gaactgttca ccggacacac catgttcctc acctttgggg
    26461 taaaagcggg cagcatcagc cacgggcaaa tgtgggcaat cctgccgcaa acctggctgg
    26521 gtaacctggt cggttccgtc ttcgttgcca tgctctatag ctggggcggc ggtagcctgc
    26581 tgccggtaga taccagcatc gttcactccg tcgcgctggc taaaaccact gcaccggcaa
    26641 tggtactgtt cttcaaaggt gcattgtgta actggctggt ttgcctggca atctggatgg
    26701 cgctgcgcac tgaaggggcg gcgaaattta tcgctatctg gtggtgtctg ctggcattta
    26761 tcgcgtccgg ctacgagcac tctatcgcta acatgacgct gttcgcgctc tcctggttcg
    26821 gcaaccacag cgaagcctac acgctggcgg gtattggtca taacctgctg tgggtgacgc
    26881 tgggtaatac tttatcaggt gccgtattca tgggattggg ttattggtat gctacgccga
    26941 aagcgaatcg tccggttgcg gacaaattta atcaaactga aacagctgcc ggttaattac
    27001 taaggggttt ttacgtggat catttgccta tattttgcca attacgcgat cgcgactgtc
    27061 tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag gttgctgtta gacgcaggcg
    27121 ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt caccgcatgg gcagatgcag
    27181 gcatgttaac cctcgtcgaa gggccatttg atgaaagcct tctcgacacc tgctggctgg
    27241 cgattgcagc gacggatgat gacacgctta accagcgcgt cagcgaagcc gctgaagctc
    27301 gtcgcatctt ctgtaacgta gttgatgcgc cgaaagccgc cagctttatt atgccgtcga
    27361 ttattgaccg ctcaccgcta atggtagcgg tctcctctgg cggcacctct ccggttctgg
    27421 cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgtt gcatctgggc caggtggcga
    27481 aatatgccgg gcaattacgc gggcgagtga aacaacagtt cgccacgatg agtgagcgtc
    27541 gccgtttctg ggagaaattg ttcgttaacg accgcctggc gcagtcgctg gcaaacaacg
    27601 atcagaaagc cattactgaa acgaccgaac agttaatcaa cgaaccgctc gaccatcgcg
    27661 gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg gctgctgacg ctgaaaggac
    27721 tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg tctggtttct gacgatatta
    27781 tgaatctggt acgccgcgat gctgatcgcg ttttcgtcgg caaacgcgcg ggataccact
    27841 gtgtaccgca ggaagagatt aaccagatcc tgctgcggga agcgcaaaaa ggcaaacgcg
    27901 tggtgcggct gaaaggtggc gatccgttta tttttggccg tggtggcgaa gagctggaaa
    27961 cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg tattaccgca gcttctggtt
    28021 gctctgccta ttcgggtatt ccactcacgc atcgcgatta tgcccagagc gtacgcttaa
    28081 ttaccggaca cttaaaaacc ggtggcgagc tggactggga aaacctggcg gcagaaaaac
    28141 agacgctggt gttctatatg gggttgaatc aggccgcgac tattcagcaa aagctgattg
    28201 aacacggtat gcctggcgaa atgccggtgg caattgtcga aaacggaacg gcagtcacgc
    28261 agcgcgtgat tgacggtacg ctcacgcaac tcggtgaact tgctcagcaa atgaacagtc
    28321 catcgctaat tattattggt cgggttgttg gcctgcgcga taaactgaac tggttctcca
    28381 atcattaatt taacccgggc cagagaattc tggccttctt aatgactctt tttatatatt
    28441 cagcaaataa aacataaccc cttaataaat attatctgct aatgggttat attcaccgtt
    28501 acagcgattt acaaaattaa gtcattcgct cactgacgcc aattaaatat tcctgtatcg
    28561 ttcttaacga gcagtctgct tatattcata taatcaatga atattaatta atatataata
    28621 catagggaat gtaaatgaac aaatttatta aagttgcact ggtaggtgca gtactggcta
    28681 cgttaactgc atgtactggt catattgaaa accgtgataa gaactgctct tacgactacc
    28741 tgctgcaccc ggcaatttct atttctaaaa tcattggcgg ttgcggtcct actgcacagt
    28801 aagtctctga cagaaatcgg ctaacaccgg tttaattgcc cgacgccctc tggattttcc
    28861 gagggcgtat ttgtttctat gtccttcttg cccccgcccg taaaaatatt ttcactttaa
    28921 attcaatttt ataactacat ccattagcca cagcgcgatc ctgtgcgaaa ttttgtgatc
    28981 ttcctccaca tcacataaca taatatgttg ttatattcat catgcattgt catgttacct
    29041 tttaaatggc tgcaaactct cccctacaac gtattggaca agaaaaaggt atcgctatgg
    29101 gaagccagga actccaacgc aagctcggat tttgggccgt tcttgcaatc gccgtcggga
    29161 caaccgtcgg ctccggtatt tttgtatctg tgggtgaagt ggcaaaagca gcgggcacgc
    29221 cgtggcttac ggtgctcgcg tttgtcattg gcgggttaat tgtgatcccg caaatgtgcg
    29281 tctatgcgga actatccacc gcttatccgg aaaatggcgc agattatgtt tatctgaaaa
    29341 atgccggaag ccgaccgctg gctttcctct ccggctgggc cagcttctgg gccaacgatg
    29401 cgccgtcatt gtcgattatg gcgctggcga ttgtcagcaa tcttggcttt ttaacgccta
    29461 tcgatccgtt gctcggtaaa tttatcgccg ccggattaat tatcgccttt atgttgctac
    29521 acctgcgctc cgttgaaggc ggcgcagcgt ttcagacgct aattaccatc gccaaaatta
    29581 tcccgttcac tatcgtcatt ggccttggga tcttctggtt taaagcggag aattttgccg
    29641 cccttaccac cactgcgatt ggcgcaacgg gcagctttat ggcgctgctg gcggggatct
    29701 ctgccaccag ttggtcgtat accggcatgg cctctatctg ttatatgacc ggcgaaatta
    29761 aaaaccccgg aaaaaccatg ccacgagcgc tgattggttc ctgtctgctg gttctggtgc
    29821 tctacaccct gctggcgctg gtgatttccg gcctgatgcc cttcgacaaa ctcgccaatt
    29881 ctgaaacgcc gatttccgac gccctgacct agatccccgc actcggcagc accgctgggc
    29941 tctttgttgc catcacggcg atgatcgtca ttcttggttc gctttccagc tgcgtgatgt
    30001 accagccgcg gctggaatac gcgatggcga aagacaacct gttctttaaa tgcttcggcc
    30061 atgcgcatcc gaaatacaac acgccggatg tctccatcat cctgcaaggg gcgctgggga
    30121 tcttcttcat cttcgtttcc gatctcacca gcctgctggg ttatttcacc ctggtgatgt
    30181 gtttcaaaaa taccctcacc ttcggctcca tcatctggtg tcgtaaacgc gacgattaca
    30241 aaccgctgtg gcgtactccg gctttcgggc tgatgaccac cctcgccatt gcgtcaagcc
    30301 tcattctggt cgcctcaacc tttgtctggg caccgattcc cggccttatc tgcgccgcca
    30361 tcgttattgc tactggtctg cctgcttacg ccttctgggc gaagcgtagc cgccagctca
    30421 acgctttgtc gtaatgttat ctgaagaaaa taaaatgttg gatattgata aaagcaccgt
    30481 ggactttctg gtcaccgaaa atatggttca ggaagtggaa aaagttctca gccatgacgt
    30541 tccgctggtg cacgccatcg tggaagagat ggtgaagcgc gacattgatc gtatttattt
    30601 cgttgcctgc ggctcgccac tcaacgcggc gcaaacggcg aaacatctgg cggatcgctt
    30661 ttccgatctt caggtctacg ccatttccgg ctgggagttc tgcgataaca ccccgtatcg
    30721 cctcgacgat cgttgcgcgg tgattggcgt ttctgactac ggtaaaacgg aagaggtaat
    30781 caaagcgctg gagctgggcc gggcctgcgg agcactcact gcggcgttca ccaaacgcgc
    30841 ggacagcccg attacctcgg cggcggaatt tagcattgat tatcaggccg actgtatctg
    30901 ggaaattcac ctgctgctct gctacagcgt ggtgctggag atgatcaccc gcctcgcgcc
    30961 gaacgcggaa atcggcaaga tcaaaaacga tctcaagcag ttgccgaatg cgctcggtca
    31021 tctggtacgc acctgggaag aaaaaggccg ccagcttggt gaactggcca gccagtggcc
    31081 gatgatttat accgttgctg cgggtccgct gcgtccgctg ggttacaaag aaggcattgt
    31141 aacgctgatg gaatttacct ggacgcacgg ctgcgtgatt gagagcggag agttccgcca
    31201 tggcccgctg gagattgtcg aaccgggcgt tccgttcctg ttcctgctcg gcaatgatga
    31261 aagtcgccac accaccgaac gcgccattaa ctttgttaaa cagcgtactg acaacgtgat
    31321 cgtcatcgat tacgccgaaa tttcgcaagg gctgcacccg tggctggcac cgttcctgat
    31381 gttcgtgcca atggagtggc tctgctacta cctgtctatt tacaaagatc acaacccgga
    31441 tgaacgccgc tattacggtg gtctggtgga atattaatcc ctctcccggc ccggcaacgg
    31501 gccggattta cgcaaggagt tacccgatga aaacaggtat gtttacctgc ggccaccagc
    31561 ggctgcctat tgaacacgca tttcgtgatg caagcgagct gggttacgac ggcatcgaaa
    31621 tttggggcgg tcgcccgcac gcgttcgcgc cggacttaaa agcgggcggc atcaaacaaa
    31681 tcaaggcgct ggcgcagacg tatcagatgc cgattatcgg ttacacgcca gaaaccaacg
    31741 gctatccgta taacatgatg ctgggcgatg aacatatgcg tcgtgaaagc ctggacatga
    31801 tcaagctggc gatagatatg gcaaaagaga tgaacgcggg ttatacgctg atttctgcgg
    31861 cccacgcggg ctatctcacg ccgcctaacg ttatctgggg gcggctggca gagaacttaa
    31921 gcgaactgtg tgagtacgcg gaaaacatcg gcatggatct gatcctcgaa ccgttaacgc
    31981 cgtatgaatc gaacgtcgtg tgtaatgcta atgatgtgct tcatgcgctg gcgctggtgc
    32041 cttcgccgcg cctgttcagc atggtcgaca tttgcgcgcc gtatgtccag gcggaaccgg
    32101 tgatgagtta tttcgacaaa ctgggcgata aattacgtca tctgcatatt gtcgacagcg
    32161 acggggccag cgacacgcat tacattcctg gagaaggcaa aatgccgctg cgggaactga
    32221 tgcgcgatat tattgatcgg ggctatgagg gttactctac ggtggagctg gtgacgatgt
    32281 atatgaacga gcccagactc tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc
    32341 cggaggatga gagatgaaaa ccctggcgac aatcggcgat aactgcgtcg atatctaccc
    32401 gcaactgaat aaagcgtttt ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg
    32461 ctacggcata cagccgggat gcattacctg ggtgggtgac gatgactacg gcacaaagct
    32521 gaagcaggat ctcgcccgca tgggcgtcga tatcagccat gtccatacga aacacggcgt
    32581 taccgcacaa actcaggtgg aactgcacga caatgatcgc gtttttggcg actacaccga
    32641 aggcgtgatg gccgactttg ccctgagtga agaggattac gcctggctgg cgcagtatga
    32701 cattgtgcac gcggcaatct ggggacatgc ggaagacgca ttcccacagc tgcacgctgc
    32761 gggcaaactt accgctttcg acttctccga caagtgggac agcccgctct ggcagacact
    32821 ggtgccgcat ctcgattttg cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct
    32881 gaagatgaaa gcgattgttg cccgtggcgc aggaacagtg attgtcacgc tgggtgaaaa
    32941 cggcagcatt gcctgggatg gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt
    33001 tatcgacacc atgggtgccg gagattcgtt cattgccgga ttcctttgcg gctggtctgc
    33061 ggggatgaca ttaccgcagg cgatggcgca gggaacggcg tgcgcggcga aaaccattca
    33121 gtaccacggt gcctggtagg tataacgttg gcgtgagcat cttcacgcca acgtgctgtt
    33181 acttgccgga aaacgaccct ataatccgag taattcattc tttatttcag ggtcgattat
    33241 gtcagctacg gaccgctact ctcatcaact cctctacgct accgtccgcc agcggctgct
    33301 ggatgatatc gcgcaggggg tttaccaggc cgggcaacag atccctaccg aaaacgagct
    33361 ttgtacacaa tataacgtca gccgcattac cattcgcaaa gccatcagcg acttagtggc
    33421 agacggcgta ctgatccgct ggcagggaaa aggcaccttt gtacaaagcc agaaagttga
    33481 aaacgccctg cttactgtca gtggttttac cgattttggc gtctcacaag gcaagtcgac
    33541 gaaagagaaa gtgatcgaac aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa
    33601 catccccgga aacagcgaag tgttccatct ctgccgggtg atgtatctcg ataaagagcc
    33661 gctgtttatt gatagttcat ggatcccgct gtcgcgttat cctgactttg atgagattta
    33721 cgtcgaagga agctccacct atcagttatt tcaggagcgt tttgacacgc gagtggtcag
    33781 cgacaaaaag accatcgata tctttgccgc cacccgcccg caggcaaaat ggctgaaatg
    33841 cgaactgggc gaaccgttgt ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc
    33901 ggtgcacgtc tccgaactct tctgccgcgc caatcgcatc accttaacga ttgataataa
    33961 aagacattaa ccgtaggccg gataagatgc gccagcatcg catccggcga tgctggcgca
    34021 ctgaatttta catcccgcac gttcccctca ccctaaccct ctccccaaag gggcgagggg
    34081 accgttcagt gcggaattta gcttgattgc cgaacggtgc tatccagcat taaatactcg
    34141 caatactctc acgcaaaatc cgcagcaccg tctcttcacc gctgcgattc gggttaatgc
    34201 gaatcgcaca atgttcgagt tgtggattcg cctggcgaaa cgttcctgaa agtcgataga
    34261 agagcggcgg gatttcatat ttcgactctg caccgaccgg gtaaggcaac gcgccacgct
    34321 tttgcgcttc ttccagcacc ctggcggcaa tcagctgatg aaactcgaca atcaacacct
    34381 tcgactgcgc attggcaatg acggcgcttt tcacttccgc caccgcgccg ccgttaagca
    34441 aagccagcaa ccgttcggat actcccgcct gcaccgcatg catcactggc gcgaacacca
    34501 gaccacgcaa gacttccagc gcctgtgagc cctggatctg gctaccgccg gagtaaagcg
    34561 tggcgcgaat ccgactaata acatcggcat cgccaaccac tgcaccaacg ccctccggcc
    34621 caaacagctt gaagcaggag aatgtcgaga cattcgcgcc gcattcacag ccgattcgcg
    34681 ctaccttcat caccgcatag ttatcatcgg ttaacgctgg aacacctgcc gcgcgcaacg
    34741 ttgccagcac atctgccagc acatagctgt cctgcggctg ctggcgcgta tgctgcacca
    34801 gcgcggcatc aggttgttgc tcgtcgacga cctgcttcag tgccgacagg tcattgaaat
    34861 cagcagtaat aagcgtcagc cccatctgct caataataac ccgtgtcgtc gggtaaacag
    34921 gcgcgtcatg caccagaaga cgctgccccg gtttgagcaa agccgccagc ccggcgcgaa
    34981 tcgcgccagt ccccgcgccc tgcaccagcg ccgcggcctg tgcgtgaaat gcatcagcca
    35041 gcacctgctc cacccgttgg gtaatacgcg gttgattcag acttggagtt aaccctaaat
    35101 caccgccagc aagaaactcg ctgcctggaa aatggcggca aatagtgtcc accagcgcaa
    35161 actgcttttg ctgcgcttca gcaagagtca ggctttgcaa aggaaacgtc ttcataggtg
    35221 ccttccttaa gccggaacga acaggccaag ccagtaaaga acattcagca gaataccggt
    35281 gatcattact gccacgaccg gtgccgccat tttctgtacc ggacggccca gggattcgtt
    35341 gaggaagtaa atggcaaccg cgatagagaa tccggtataa cccgccatct taattgctgc
    35401 gaaaatcgaa ccgaccagca gcgccacttc catcagcata ttcatggcgt tacggatgtt
    35461 atccgacgca ttacgcaccg acgggtagcg tcccagccat ttgccgatcg aacgaagcag
    35521 caagacttcc gccgaaataa ccactgcgcc taataccgct gcaaccatcg gattcggcga
    35581 gagatagccc accgcataaa caaaggtaaa gcccgcaact gcatacacac ccgttgctaa
    35641 ggcggtggtg gcaatcaacg gcacaaaccc cagtccgcgc ataaattctg ccagagccgc
    35701 ctgattaatc agcgtttgcg attgttccgg cgtcacgcct gcggaatacg ctttctccag
    35761 tgtgaagatc gaaacttcgc taccagcaaa aatcttcata ctggcaacgg cggcaatcaa
    35821 tgctcccacg atggcgatat aaggtaagtt tttgataatc cgcgacgtgc gctcttcaaa
    35881 caccgaaagc ccgctggcat cgatgtcatt ttcatcacga tgacgcaggt cgtgagttat
    35941 cgcgatcccc agcagcatca ccatgccaat aaagatttcg atggattcag ggttaagatg
    36001 tgggaaatag cgcacgacta ccacacgggt catcagaaca accacggcgg cgaccagact
    36061 ttgcttccag ccaaactggt agaaaatcgc caccagcggg aacagtgcaa aagcagaaac
    36121 caccggcgag cttaattccc cgaggctacc taatacatcc accggcagcg cggtcagcag
    36181 ctggttaact ggcaacaggc aagtaaggat caacacgccc cagatagcgc caagaccaaa
    36241 cgccatcagg ctgtttatcg ccagtacgcc gaggatatcg gtaggaagaa agagtaacca
    36301 tgcgttgagc agcccggttt tcagggtaaa agagatcccc accgaggcca caaaaccaat
    36361 gctcagacca aaagcgatac tccccgcctc gcgacggttc atatagcctt caatcagttg
    36421 cggcaggatc gggtggatgc cgtcatgaaa aacagccgcc gagcgatgct ccagaagcga
    36481 tgtcataccc gtcaggcacg ccaccacgat aatctgaata tacagatcca tagtcgtgcc
    36541 ttttatttca gatggttaat caacatcgga atcgcgtgtt cgacgtgctc aacggaaagg
    36601 ccaaacgcca ctttcccttc agcgatcatt ttggcgatat gctcgtcttt cgctttaatg
    36661 cctggtttgg caatggtgca acttttgtta tagccaatca ccgcgatggc aatcgacaat
    36721 gccgcgcccg caccggtatt acacgcgcca atgtaataat ctagttgccc ggatttcacc
    36781 ttcattgccg cttccatgtc gttgtgaatg aaaacttcaa aacagccagg agccgtcgct
    36841 tcaatggttt ttttaatctg ctcacgctgt aagcctgcaa cgccaatctt tttcatgagt
    36901 ctgtccttat tggaaaaatt gagagggatt ttcacgtaac atcacatcca catcggcctg
    36961 actgaatcct gactggcgca attgcggaat aaaggtggtc aataaatagt catagccata
    37021 accaccattg gcttttaaat gggagcggcg cgtaatatcc atcgacagca tgacgcggtt
    37081 cagcaaccca cggtcacgta gcgcgtggag catcgcaatg cgcttttcgt ccgggtagta
    37141 actgttctta ccaatggtgt cgaactgcac gtacgcgccg agatcgatca tcttcaaaat
    37201 gttgtcgagg ttgtctttca gatcgcagtg accaacggtg atgcgcgaaa gatcaactcc
    37261 gtgggcttgc aacagagcca gttgttccag ccccatggtg ctgaacgacg tatgcgtgga
    37321 gatcgggcgt ccggtctggt tatgcgccag cgcagcggca ataaatacct tctcttccag
    37381 cggcgtaatc tttccttcgc tggagccgat ctccgcgatg atcccggctt tcagctccgt
    37441 gccatcgata ccctgttcaa tttcatcgac catctcctgc gccagttcct gcacgctgcg
    37501 ggtcaccaca tgttccggga aaaacgcatc ctggtaataa ccagtacagg ccaccacgtt
    37561 gatcccggtt tcgcgcatta catcgagcat aaattgcgca ttgcgcccca tgtaacggtt
    37621 ggtcatctca atcacattac gtacaccccg ggtcatcagg tcgttcatct cctggcaaat
    37681 gaacgcatac tgatcaaggc ggcagtccac gttgttttta aagccggaga gatcaatatg
    37741 cagatgctca tgggccaggg tgtaacccgt cggatcaaaa ctcatagggt gtctcctgca
    37801 aaccggaggg aggaaagaaa agagcgacca ttttgtggcg gtggcgcacg gaaaaattca
    37861 cacacggtag ctcccacatc agaaagcgtg gtgcgcacac cgagctgcgt ggcgaccagc
    37921 ccttgctgat aaaccagcac tggcaccact tcacgggtat ggtggctgtg accaatggtc
    37981 ggatcgttac cgtgatccgc catcacgacc aggcaatcat ctggctgcat cgcctcaaca
    38041 agccgggcaa ggttacggtc aacgacctgc aaacgttcgg cataacgtgc gacgtcttct
    38101 gcatgaccag cgaggtcggt ttcctggata ttgatgcaaa taaacgccgt cggatgggtg
    38161 ttaaattcgt cgagggtgat atccataatc cgctggctat ccaccaggtt ttgccagctc
    38221 acaccataag gattgctgac gatatctgcc accttaccca ccagcacggt tggcacgccc
    38281 gcttcataca gtttttgcgg cacctgcact ttttcatcaa caccgtagcc catatgcacg
    38341 acctggaagc cgttgtcata agcgccagaa cgcggcgcat taataccaat aaagcgccct
    38401 tctttgcttt ctgcggcatc gagaatacgc tgactgttgg ttaacaggcc gccaaatgta
    38461 atgacccgac cgacctgtac ctggtcacgc acgatacgac caattttgat tgcgtcgtca
    38521 aaagagatca cagagagatt ggcggtaatg ttatagactt ggcctaaatc cgcctcgaga
    38581 ttatcgccaa tcgcaaccgc ctgattgacc gacagaaatt gcagatcatc gccacggcgc
    38641 tccacctgcc agccagcgga aactaatgcc tgctcaacac ggtcaatcac atcgcgaaaa
    38701 ggcatccgca gcggcggtaa cgggcgcgtg cctaaaattt cctgatgccc cataaaggta
    38761 tcgccacctt catgttgcag ctctgccacg ccccaggttg cggaatctga cggctgcata
    38821 tcgcctggcg cataacccaa tgtgttgatt agccccagct tctccagcgt tggtagctgc
    38881 aaatgcggca actggctcag gatgtgacca caggtattcg ccccggcatc ttgcgggcgc
    38941 accagcgtga catctttcat tgcccctacg ccaaagctat caatcactaa caccacaaat
    39001 cgcgccattt atgcctccag agggttgccg aggctgtcat aacgaccaac tatttctggt
    39061 tcgccgcgct gaataccgga caccagcacc acatcgctac gggtgacaaa aatctgcgtg
    39121 cgaaaacaga gcaccactgc actgctcacc ggatactcgc ctgccagcgg cagggtgtag
    39181 tcgatactgc tgtcatccac agttttgaga ttggtttcag taatcttttg attttctggc
    39241 gtaaacacca gcgcatgttg cgcatgacca cgacggtagt aaccgccgcc gtagcagtag
    39301 ctgtcgccac ggaaatgatg ggagatttca cttaaccaga gcatcgcgat acgttcaggc
    39361 tgatcgccct gctggtttgc cggaatagta ccggtcagcg catgaccggg ttcggcatga
    39421 gtcgcaccgt attccgccag caatggcagc gaagtgcagc tggtcgctga aggtacgttc
    39481 agttgctcaa tcgcaatacc agatttcgcc agttgatccc gtgcttgtac cagcgtgtga
    39541 agattcggtg ttggcaaaac tttcccagca gcctcatccc aaagcaggca agggaaatgg
    39601 gttagtccgg ctaaatgcag ccccggcagt ttcttaattt cagcaatcac ctcgtgcagc
    39661 gaatgctgta caaaaccgct ctcctgcccc ggataaagaa aatcatcgtc gctataaact
    39721 ttgagcagca caggctggac ccgtccggcc ttaaccgctg ccgcagaaac ttcccgcgct
    39781 ttgtcgagag taaacacggt gatgatgtcg gtgccctgtt caacggcgtc agcaacctga
    39841 tgacaaggga tttgtaccag atgcccctga tgcgccactg gcaaaccagc gcggcgcatg
    39901 actcgcgcct ctttgtaatc caccgccaca atgccgctgt agcctaatgc cagcaatttt
    39961 tccgccagcc acggattacg accaaattgc ttggtcatca gatacagttc aatgccgtaa
    40021 agccgcgccg tctcaatgag ccgcttaccg ttttccagta tctgatccac gtcgatcacc
    40081 cagctgtccg gggcgatttt gccctgctgc cacaaactta gtgcggcgga aatcagcgcc
    40141 gggttctggc gtttcaatgc ttctacaaac atcttgttgc ctcgtttctg ttccttgaat
    40201 taaatattca tttttttgaa tatttaggtt gtgcgacgtg gatttgtcgg gagcttcaaa
    40261 cctcttcgtt agcagccatc cataatccat aaagattggc cagaaaatag ccttcttcac
    40321 acgggttaac ttccagagcg aattccttca acaactcctg atgcaattgc acaacggctt
    40381 gccagtggct ggattgcgcc agttccgcca gcaactcgtt atccagcggc tctatttctt
    40441 caccacggcg actgcgcatc agtgcactcg ccatatgtgt catcgccatc gttccttgct
    40501 cactgcgcac cggcagatgg cactcttttt ccagtacgtt gacgacctgc atcatgcctt
    40561 tgcagacgtc cttatcaata acgcctgcct cgcaaagcag gttgagtctg gtttccatat
    40621 cagatgcctt tcgtttaata actgggttcc tgttccccac tcaccacccg ttgttgatgg
    40681 gcaagcaggg cgtgtttatc tacaacagca cgcagcaatt gttgcattgg gtaatcatcg
    40741 actcgcgtca ggaccacggc ttcggtggcc tgtaaaaatc gcggatcgtc tgttagcggc
    40801 gtcgcctcta atcccagcat ggtcagttcg ttttccgcca ccacgttcca gatcaccgca
    40861 tcgacatcgc ctttaacaat gcgttgtaaa ctctcgtgat aagagagatc gactcgttcc
    40921 acatcactat cgccaaaaaa gacatcggtc atgattttct gatccgccga acgactatcg
    40981 agacctactc gcttaacatt tccagactca cctttgcggc atatcagttg atgttcacca
    41041 acatacgtat gcgggccaag ctcgagggcg atgcacaaat tgttttggct gagataactt
    41101 tccgccgcca ggcgggaaac tactgccatg tcatagacac cgttaagtaa acactccacg
    41161 cgaatatccg cgccgcgcat atgcgcatag taaaaaggaa tgccatcaaa ctgggctttc
    41221 aatccgctcg ctaaaccttc gtacaagcgg gtatagggca aaggcatcgc acataccacg
    41281 ttgttgatat ccacatgact cagcaatgct ttgttatcca tctcgacaag ataactgcca
    41341 ttgcgcccac ggcgttcaat ccgtatcgct ccgcttgatt ccagcgtttt caacgcggcc
    41401 tgcgtcaggc caaccgatga acggcattca gttgccagct catctatggt tttcagtcga
    41461 ttaccgcatt tttcacccaa caaataacgg gccagtgtcg tgatgacgac gccttctttt
    41521 ttgataaacg ttcgacgcat aatatatttt caataaaatg aatatttata tcttcattaa
    41581 aatgaagata agtggcgacg acgagatgtg gaaacggcga ggcgcttcac attttttcgg
    41641 gttgttgcga aaagaggata aaaaaaccgg gtttccccgg tttcagagtg atgacgaagt
    41701 gatttattcg acaatgcgag caaagggtaa ttcccactgg cgcttgcctg ctcccctcac
    41761 cctaaccctc tccccaaagg ggcgagggga ccgtccgagc gttttgatat tacggcttcg
    41821 ccacaaaacc aatcgcttcg tacaccgctt tcagcgtacg ggaagcgtgt acactggctt
    41881 tttctgcgcc gtctttcatc acctgttgca ggaaggcttc atcgttgcgg aaacggtgat
    41941 agcgttcctg caattcagtc agcataccgg aaacggcatc agccacttca cctttcagat
    42001 gaccatacat cttgccttcg aactgttttt ccagttctgg gatgctctgg cccgttaccg
    42061 cagaaaggat atccagcagg ttggaaacgc ccgctttgtt ctgcacatcg tagcgaacta
    42121 ccggcggctc gtcggagtca gtgaccgcgc gtttgatttt cttcactacc gatttcggat
    42181 cttccagcag gccgataacg ttattgcggt tatcgtcaga cttggacatc ttcttggtcg
    42241 gctccagcag cgacattacg cgcgcgccag atttcggaat aaacggctcc ggcaccttaa
    42301 agatctcgcc atacagcgcg ttgaaacgct gggcgatatc gcgactcagt tccaggtgct
    42361 gtttctggtc ttcacccacc ggtaccagat tagtttgata cagcaggatg tccgcagcca
    42421 tcagcaccgg atagccaaac agaccagcgt tgatgttctc ggcataacgc gcagatttat
    42481 ctttaaactg cgtcatgcga ctcagttcgc cgaagtaggt atagcagttc agtgcccagc
    42541 ctaactgcgc atgttctggt acgtgggact gaacaaaaat ggtgcttttc tcaggatcga
    42601 taccacaagc cagatacaag gccagcgtat ccaacgtcgc tttacgcagc ttctgtgcat
    42661 cctggcgcac ggtgatcgcg tgttggtcaa cgatacagta aatgcaatgg tagtcatcct
    42721 gcatgtttac ccactgacgc agcgcaccca tgtagttacc aatggtcaat tcacctgagg
    42781 gctgtgcgcc actaaaaacg atgggcttag tcatttttcg attcctgatt ttcgctatgc
    42841 ggaagcccta atgcgggcag aaggtcattt atagactgat aaattacatc aggctggctg
    42901 agatcgatag cctcgccgta gttgtatccg taggttaagc caactgatgg gcaacctgcc
    42961 gcttttgccg cctgaatatc attccgtgag tcgccgacaa acagcatctg ttgtggggca
    43021 attcccatcc gctcagccac cagtaacagc gggtccggat gcggtttttt gttttgcaca
    43081 tcatcgccgc cgataaccac gctaaagtat ttggcaatat ctaaggcttc gagcagcggc
    43141 gcgacgaacg gcgtcggttt gttggtgacc aagcctagcg gcaggccttt agcctgcaac
    43201 gcgcccaacg tatcggcaac gtgcgggaac aaaaacgtcc cctcttcggc aacctcgcca
    43261 tagtagcgat cgaacagttt acgcagaata cgtacctgtt cttctgccgg aatgtcgtca
    43321 tcaacgggcg gtttccccat tgttttacgc agagtcgcac gttcctgacg cgcccaggtc
    43381 aatgcgcgct ccatcagaac atctgcgccg ttaccaatcc aggtaataac gcgttcttca
    43441 cctgcgacgg gcaactccag cgcatacagc gccatatcta ccgcagcagc aagaccagga
    43501 gcactgtcga ccagcgtacc atcaagatca aaagcgacgc cgcgaatatc ttcaaactta
    43561 ttcatgactt acctttgcca gttcactgcg catttcatca atgacttttt tatagtctgg
    43621 ctggtcgaag attgccgaac cggcgacgaa catatccgcg cccgccgcag cgatttcgcc
    43681 aatgttgttc accttcacgc caccgtccac ttccagacga atgtcaaagc cagactcgtc
    43741 gatacggcga cgtacttcgc gcagtttatc cagtgtttga ggaatgaaag actgaccgcc
    43801 gaaaccaggg ttgacggaca tcagcaggat cacatccagc ttatccatca cgtaatccag
    43861 atagctcaga ggtgtcgccg ggttaaatac cagacccgct ttacagccat tttctttaat
    43921 cagttgcagc gtgcggtcaa catgctcgga ggcttctgga tgaaaggtaa tgatgctggc
    43981 accagcggca gcgaaatcag gcacaatgcg atcgacgggt ttcaccatca gatgtacgtc
    44041 gataggggcg gtaatgccgt agttacgcaa ggatttcagc accattggcc caatcgtcag
    44101 attgggaaca tagtggttat ccatgacgtc aaaatgcacg acatcagcgc cagctgccag
    44161 ggcttttgcg gtatcttcac ccaggcgggc aaaatcagcc gacagaattg agggggcaat
    44221 caaatactgt ttcatccgct tctccttgag aattattttt tcgcgggtga aacgactcct
    44281 ggtttgtaca aagccagcag ttcgtccacc tttttacgtg tgccgccgtt gctgcttata
    44341 ctgcgtcgaa ctttgacgac atgcaatttt gcgcgctgat accactcacg cgttaacatc
    44401 gtatcgtgat tggagatcag cactggaata tggcgctcaa ccagaccttc ggcgatctcc
    44461 gccagatgcg cttgttgttc aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg
    44521 gtcgcagaca gcggtgcata aggcggatcg caatagacga cggatgcatc atctgcgcgc
    44581 tccatgctat cggcgtaaga ctcacaatag aaaaaggcat tctgcgcttt ttcagcgaag
    44641 tgatacaact ctgcttccgg gaaatagggt tttttgtagc ggccaaacgg cacgttaaac
    44701 tcaccgcgca gattgtaacg acacaggccg ttgtaaccgt agcggttcaa atataaaaac
    44761 agtaccgccc gacggaacgg atcctggctt ttgttgaact cttcgcggaa ctgatagtaa
    44821 acctcggcgc aatttgtttc gggaacaaac agctcgcgtg cggcctgtac gtactcatca
    44881 gtacgcatct tcacaatgtt atagagactg atcaggtcgc tattgatatc ggcaaggata
    44941 taacgagaaa agtcggtgtt gagaaacacc gacccggcac ctacaaaagg ctcaaccaga
    45001 cattcgccct tgggcaaatg ccgtttaata tcatcaagca ggggatactt gccccctgcc
    45061 cacttcaaaa aagcgcgatt tttcttcatg ccgattaact aattacacct tctccggctg
    45121 tggagaaagc tccgacagca tccagcgctt caggcagctc ccgcaacgat aaaccattgc
    45181 gggagataac cttgattact tcagatcggc ctgtacctga cgcagcggtt tcgcccacgg
    45241 gtttttggcc tgaacatctg ctggcaatgt agataccgct tttttcgcct cttctttcga
    45301 agcgtacacg ccagaaacca ggacatacca cggctgacca ttacgcgtcg tttcatagac
    45361 aacgtagttt ttcagattct ctttcttcgc ccaaccgttc aggttgtcgt agttagagga
    45421 actgctcagc tgcagagtgt aatggctgga cggtgccgat ttcaacgaac caacattacc
    45481 tgcggtcttc cctccagcgg ctggtgttgc cgtggtttgc gccggggatg ccgtctgtac
    45541 tggagccgta gtcgccgtct cttttggtgc tggcgtagaa gtcgcagccg gtgccttcgt
    45601 gctcgccaca ggggcagctg gttcaggttc agtacgcttc ggcgtctgtg ctaccggctt
    45661 cggctccgtt ttcacggttg cttgcggttt tttcggttca atcaccgcct gctgacgaac
    45721 tgggcgcgta gtggacggac gttcagcggt ttgcgttttc gccgtgtcac gcgatgcatt
    45781 gccattgcga acaggcgcaa ccgttgcggg ttcagtcggc aacgtggaat tgaccgccac
    45841 attgttcagc tgttgctgat tttgtggctg ggtcagcgca ttgttcaggt caccctgcac
    45901 ttcaacacgt tgttgaccat ccgttgccac cggggtttgc ccttgagtcg gcgtagaaga
    45961 gatcggtggc agagaaacat cctgctgagt attttccgca gacgtggttc ccggcgctgg
    46021 ctgcacgcca ttcgcctgat cggtcgcatt gccagcaaga tcaatactct tctcgccaga
    46081 cgcggtttga tcgctggaag tggtcgaggg ggcttttagc gcagaaccga taccgatgat
    46141 caacagcagt agaaccagaa tgccgacgcc catcatcata tactgacgag aagcgggttt
    46201 actggctgct tttttgcgct tacgcggacg acgctctacg cgctcttcat ccacggtttc
    46261 atcttcggat tcgtcaattt cttcttcgat ttccggttcc tcattgcgct ctttttgcgc
    46321 acgagtcgga cggcgatcgt cagtgtcatc aagttcaata tcatcaaaat tgatctgcgg
    46381 ttcgccacgt tcagtacgct cagaacgttc agaagattga cgagaacgac cagtacgacg
    46441 atcgctggga tcgggtttca gctcgtcttc tggtttgaat tcatccattt aacaccccac
    46501 taaaaggtta atgcttacca cgttgcaatt aacctgaagc taatagaccg cttgataagc
    46561 ggcctgacct ttcttgttgt tacgctgatt gacaatcggc aatggcgtta agaacaagct
    46621 cgtgcgaaac gccgctgcga acttcactct taccaattgc caacggaaga attaagcgca
    46681 tctctcccgc aaggactttc ttgtcacgca gcatatgcgg taaatacgcc tgcgcggaca
    46741 tttcgcgcgg cccattgacc ggtaacccag cccgcgtgag cagggttata atacgctgcg
    46801 tttcggcaga actaaactgc ccgagacgtt ccgacgtccg cgccgccatc accatacccg
    46861 cagcgaccgc ttcaccatgt aaccaattgc cataccccat ttcagcttca atggcatgac
    46921 caaaggtgtg tcccagattc agtaaagcac gtaacccggt ttcgcgctcg tcggcggcga
    46981 caacttctgc cttcagttca caacaacggc gaatacagta cgccattgcc ggaccgtcca
    47041 gacgcaacaa cgcatccaga ttctcttcca gccagttaaa aaacgcaccg tcaagaataa
    47101 tgccgtattt gatgacttct gccagccccg acgctaactc acgcggggga agcgttttca
    47161 gacagtcgag atccaccacc actgaagcag gctggtagaa cgcgccaatc atgtttttac
    47221 cgaggggatg gttgaccgca gttttgccgc caacggagga atcgacctgc gacagtaacg
    47281 tcgtcgggac ttgaatgaaa cgaacaccgc gctgataact cgccgccgcg aagccggtca
    47341 gatcgcccac tacgccgccg ccaagcgcca ccagcgtagt atcgcgacca tgcggctttt
    47401 gtaacaacgc cgtaaagacg gtatcgagta cagctaggct tttatactgc tcgccgtcag
    47461 ggaggataac gctatcgacg ttaacacccg cctgttcaag tacgccgcgg accttatcga
    47521 gatacagagg agccagggtt tcgctggtga ccaacatgac ctgctcgccc gatttcagcg
    47581 gtaagaatga agctggttca ttaaacaaac cagatgcgat ggtaattggg taactacgtt
    47641 ccccgagagt aacgacaatc ctctccataa cgcgacatcc accttaatta ctgtacccgc
    47701 agacgagtgt atataaagcc agaattagtt gctttccagc atgtgaataa tctggtttgc
    47761 aaccacttta gcgctttgat catcagtacg aatggtcacg tcggcaatct cttcatacag
    47821 cggattgcgt tcattggcca acgcttccag aacttcacgc ggcggtgttt caacgtgcag
    47881 caacgggcgt tttttatcac gctgcgtgcg tgcaagttgc ttttcgatgg tcgtttcaag
    47941 ataaacgaca acgccacgag cggaaagacg gttacgcgtt tcacgggatt tcacagagcc
    48001 gccgccagta gccagcacaa taccctgttt ctcggtcaac tcattgatga ccttttcttc
    48061 gcgatcgcgg aagccttctt cgccttctaa atcgaaaacc cagcccacat cagctccggt
    48121 tcgtttctca atctcttgat cggaatcgta aaattccata ttgagttgtt gagctaactg
    48181 gcgcccaata gtgctttttc cggcacccat aggcccaacc agaaagatat tgcgtttctc
    48241 tgccattttt tcggtactac taagactatt cgttaatgat aaacccgctt cgctcagaga
    48301 gcgccgcagg acatgaactg aaacctcata agatattgcg agagtcagac tgaaaattat
    48361 ctcaatactc cagcgggttt ggcaactgaa taaatcaccc atccctctgc atatctggtc
    48421 gctgcaagcg cgctgccttg ctaccaccgc tctggcgata aatcaccggg taagattagc
    48481 gtaaaaaaga cagcaaaatg ccgcctgaat gataaatcat catcatggaa cgcaggcagc
    48541 gaagagcacc aactctcaaa tcggtactcc ttgtatgcta aatacctgcg cgcgtcaaat
    48601 agatgaaaca cgttcagcat aaaaacatta ccgcttttac ggctgtttac tcactggaaa
    48661 ccagtcgtgg cgtgataaac accactaact cgcgtcgttc atcttctttt ccgtcatgac
    48721 gaaataattg cccgaaccag ggaatgtcgc caagcaacgg tacgctatcc tgacccgatt
    48781 tatttttacg ggtaaaaatg ccgcccagcg ccaacgtttc tccgcttttt acctcgacct
    48841 gcgtttcgat ctcctgctta tcaatcgcca gcacttcgcc atcggcctgc tgtagcacct
    48901 gccccggaac gttctggctg atgtgtaatt tcagccggat gcgacctttt tgtaacaccg
    48961 tgggcgtgac ctccatcccc aggacggcct ctttaaattc caccgacgtc gcgccacttt
    49021 ccccgctgga aacctgatat ggaatttcgc tctcctgttt aatgctggca ggctgaagat
    49081 gtgaggccag cagacgcgga ctggcgataa tatccagctg ctgtttttgt tcgagcgcgg
    49141 aaagctcaag atccagcaag cgtccgttga tgcgcccaat gttaaaaccg acatgcgttg
    49201 tcgccgtcgc tacggagagg tcgctaccaa gcgtggtgac ttgcccaacg ccaccagcgt
    49261 gttgcgcatc ggccagcgtc catttcacgc ctaactcacg caaacttttt tcattaatgg
    49321 tgacaatatg cgccgacagc tcaacctgcc cgaccggcag atccatttgc gctacccact
    49381 gttcaagcgc gcttaacgcc gttttgttat ctcgtagcaa aaggcgattg gtgcgtttat
    49441 cgacggtcat actccctttg gcactcagta gcttctcccc cgctttcgcc agttctcccg
    49501 cgtcggcgta ttgcagggtt atactgcgat tttccagcgg cagatttgcc tgcgcccgcg
    49561 cctgctccgc ctcctggcgg gcgatattgt cattctgcca ggcaatggaa tgcactgaga
    49621 gaatgttgcc ttcctgccgc gttatcagtc cggcgctttt cactacagtt tgtagtgcct
    49681 gcttccaggg aacatctgtt agatgtaacg acaccgtacc gctgacgtct ggcgacacga
    49741 ccaggttcaa cttctcctgt tcagccagcg cctgcaacac ctgagctacc ggaacgtcat
    49801 ccaccatcag cgtcactttt tgcggctttg ccgcctgtac gccgggtatc agcatcaaca
    49861 gtagtgcggc tatccattgc ttcatttgta tctccttgcc gttgccacaa ccattgtggc
    49921 ggttcgcagt ttgtcccggt acccagcgtt agtacgtctg gcgtcagctg taaaattgtc
    49981 cagccgtttt ccagcacatc gttttgctgc acccgtcgcc atttcttttg cccgtctttt
    50041 attacaccga tgatgcgctc gcctcgccct accatcccct gatagcgcca ctggctaagt
    50101 tcgctaatcc ggcatataga tcttccggcg atttaaaagg gtcacgcata ccggttaaaa
    50161 ggcacaatgc aatacctgcc aacaaccagc gtttaaccct catgcggcat ctccagttgt
    50221 agcgtgaaca aaagatcatc accttccacg cttaacgaaa aacggctcac gctgacgttg
    50281 cgctctgcca gccgtgtaaa tgccgacggc actgcttccc acagcgtttt caacgccaac
    50341 tcacctccct gcgcggatgg atgccaggaa accagttgcg cgccggataa ctgaaaatcc
    50401 agtggcgaaa agggcagcgt tttttcctcg ctgaagggag tggtgtctac caggcgatac
    50461 agtgcggccc actgctggtg atgacttgcc cgtagtcgaa ttaatgcgtc gcgctcttca
    50521 tggtgtgtcg atgatagaaa aatgagcgta actaacatca gcaaccagaa tgcccagcaa
    50581 aactggcgga ggcggggtga tgtggcgaac caccagtcaa agaacatgtt catcgctaac
    50641 cttccttgtt aactgatact caaattgcca gcgtccctgc gcatcctgct gcgtggctcc
    50701 ccgctgattg agatgaaaag acgcatcctg gcggagtgac gtttctagtg cgtttaacgc
    50761 ggtaatgctt gttgtcagcc ccttgatctc cagcgttccc tgctgccagc ttatcgttgt
    50821 cagccaggcg tgctcgggta aaagcgccgc cagtgcttcc agcgcagatt gccagtcgcg
    50881 ggtaaattgt cgctggcgct gccgttgcga acgctgttcg cgtaactgct gttgctccag
    50941 caaacgtggc ttcgttatct gtaagctgcg ggcgagttgt tgttccgcct gaagcaaaac
    51001 tgcgtctatg cgcgcttcgg cgctgcctgt cagacgcagt attagcgtta tcccgacggc
    51061 cagcagcaga ggcgcaacga acatcagcaa ccagaaacgc agaaaagcgg tccggcgttg
    51121 ctgtcgccag ggcaaaaaat taattggcgg attcatcagt acgccttccc aagcgccagc
    51181 cccagcgcga tggcaaagtc tccaccaggc ggcggtagcg gcggctgacg aacagaaacg
    51241 gcctcccagg ggtcaaatcc gccttcgcca catatcgcga cgctgtcggg atcaacggat
    51301 aacgctgccg ccagctcctt cgcgctagtc atccccaccg ccagtttgcg cccccagcta
    51361 tagcgcgtcg cccacagcca ctgttcgtta tcacgccagg ccagacattg ctgatgagaa
    51421 ggtaaaaaag gcaggaatcg ctgtaatgca ctggcatccg gggtgatcgc actaacatga
    51481 acacgcaacc tttctgccag cgtaagcagc gttgccagct ctttgctttg cgcggcagtc
    51541 acgttataag cggggctgag tgagtcttcg ctgtaatcga agcgcaggga gtccggatcc
    51601 atatccagct cgcgggccat cgtccctgac agccaggccg tttgctcccg ctcaccaagg
    51661 gacatcgacg ggcgcggaaa tgaccgctgt aatgtgcgac tggcgggaaa cgccaacata
    51721 atgtgatgac gctgcggcag ttcgcgactc cacggtaaca acgttttagc cagctgctgc
    51781 gcatcaacaa tccgcccatc tttgataatg tcgtgcgcca gcggcaaccg ccaccagcgt
    51841 tgcaaaaagc attcttttgc gccccgcacg atcgcaaccg ctgccgcttc ttgctgttgt
    51901 aaatgcaaac caatttgcca gatcttaaat gtcattgtga tgatctcctt atcacccgtc
    51961 actctgacgg gtatatcaat gcgtctggct tgcctttata ctaccgcgcg tttgtttata
    52021 aactgcccaa atgaaactaa atgggaaatt tccagtgaag ttcgtaaagt attttttgat
    52081 ccttgcagtc tgttgcattc tgctgggagc aggctcgatt tatggcctat accgctacat
    52141 cgagccacaa ctgccggatg tggtgacatt aaaagatgtt cgcctgcaaa ttccgatgca
    52201 gatttacagc gccgatggcg agctgattgc tcaatacggt gagaaacgtc gtattccggt
    52261 tacgttggat caaatcccac cggagatggt gaaagccttt atcgcgacgg aagacagccg
    52321 cttctacgag catcacggcg ttgacccggt ggggatcttc cgtgcggcaa gtgtggcgct
    52381 gttctccggc cacgcgtcac aaggggcgag taccattacc cagcagctgg cgagaaattt
    52441 cttcctcagt ccagaacgca cgatgatgcg taagattaag gaagtcttcc tcgcgattcg
    52501 cattgaacag ttgctgacga aagacgaaat cctcgagctt tatctgaaca agatttacct
    52561 tggttaccgc gcctatggtg tcggtgctgc ggcacaagtc tatttcggta aaacggtcga
    52621 ccaactgacg ctgaacgaaa tggcggtgat agccgggttg ccgaaagcgc cttccacctt
    52681 taatccgctc tactcaatgg atcgtgccgt cgcgcggcgt aacgtcgtac tgtcgcggat
    52741 gctggatgaa gggtatatca cccagcaaca gttcgatcag acacgcactg aggcgattaa
    52801 cgctaattat cacgcgccgg agattgcttt ctctgcgccg tacctgagcg aaatggtgcg
    52861 ccaggagatg tataaccgtt atggcgaaag tgcctatgaa gacggttatc gcatttacac
    52921 caccatcacc cgcaaagtgc agcaggccgc gcagcaggcg gtacgtaata acgtgctgga
    52981 ctacgacatg cgccacggct atcgcggccc ggcaaatgtg ctgtggaaag tgggcgagtc
    53041 ggcgtgggat aacaacaaga ttaccgatac gctgaaggcg ctgccgacct atggtccgct
    53101 gctgcctgcc gcagtcacca gcgccaatcc tcagggagcg acggcgatgc tggcggacgg
    53161 gtcgaccgtc gcattgagta tggaaggcgt tcgctgggcg cgtccttacc gttcggatac
    53221 tcagcaggga ccgacgccgc gtaaagtgac cgatgttctg caaacgggtc agcaaatctg
    53281 ggttcgtcag gttggcgatg catggtggct ggcacaagtg ccggaagtga actcggcgct
    53341 ggtgtcgatc aatccgcaaa atggtgccgt tatggcgctg gtcggtggct ttgatttcaa
    53401 tcagagcaag tttaaccgcg ccacccaggc actgcgtcag gtgggttcca acatcaaacc
    53461 gttcctctac accgcggcga tggataaagg tctgacgctg gcaagtatgt tgaacgatgt
    53521 gccaatttct cgctgggatg caggtgccgg ttctgactgg cagccgaaga actcaccacc
    53581 gcagtatgct ggtccaattc gcttacgtca ggggctgggt cagtcgaaaa acgtggtgat
    53641 ggtacgtgca atgcgggcga tgggcgtcga ctacgctgca gaatatctgc aacgcttcgg
    53701 cttcccggcg caaaacattg tccacaccga atcgctggcg ctgggttcag cgtccttcac
    53761 cccaatgcag gtggcgcgcg gctacgcggt catggcgaac ggcggcttcc tagtggaccc
    53821 gtggtttatc agcaaaattg aaaacgatca gggcggcgtg attttcgaag cgaaaccgaa
    53881 agtagcctgc ccggaatgcg atattccagt gatttacggt gatacgcaga aatcgaacgt
    53941 gctggaaaat aacgatgttg aagatgtcgc tatctcccgc gagcagcaga atgtttctgt
    54001 accaatgccg cagctggagc aggcaaatca ggcgttagtg gcgaagactg gcgcgcagga
    54061 gtacgcaccg cacgtcatca acactccgct ggcattcctg attaagagtg ctttgaacac
    54121 caatatcttt ggtgaaccag gctggcaggg taccggctgg cgtgcaggtc gtgatttgca
    54181 gcgtcgcgat atcggcggga aaaccggtac cactaacagt tcgaaagatg cgtggttctc
    54241 gggttacggt ccgggcgttg tgacctcggt ctggattggc tttgatgatc accgtcgtaa
    54301 tctcggtcat acaacggctt ccggagcgat taaagatcag atctcaggtt acgaaggcgg
    54361 tgccaagagt gcccagcctg catgggacgc ttatatgaaa gccgttcttg aaggtgtgcc
    54421 ggagcagccg ctgacgccgc ctccgggtat tgtgacggtg aatatcgatc gcagcaccgg
    54481 gcagttagct aatggtggca acagccgcga agagtatttc atcgaaggta cgcagccgac
    54541 acaacaggcg gtgcacgagg tgggaacgac cattatcgat aatggtgagg cacaggaatt
    54601 gttctgatta aaaaggcgct tcggcgcctt ttcagttggc tgacaaagtg catttgttta
    54661 tgccggatgc gggtgaacgc gttatccggc taacaaaatc gtgaaaaatc aatatattgc
    54721 aggaacccca taagcctgat aagcgttgtg catcaggcaa acttcacgca tttacactcg
    54781 cccctgccct ttcaaccatt cgcgcacgag gaacagcgcg ctgacattac gcgcttcgtt
    54841 gaagtcaggg tcttccagca aatccatcat atgcgccagc ggccagcgca cctgtggtag
    54901 cggctctggc tcatcgcctt ccagcgattc cgggtagaga tcttgcgcga ccacgatatt
    54961 cattttgctg gaaaagtaag acggtgccat gctgagcttc ttcaaaaaag tcagatcgtt
    55021 cgcaccaaat ccaacctctt cttttagctc gcggttagcg gcttcgtaga cgctttcacc
    55081 cggatcaatt aatcctttcg aaaaacctaa ttcgtaggat tcagttccca ctgcgtattc
    55141 gcggatcagg atcaggtgat cgtccacaat cggcacaatc atcactgctt cccggttggt
    55201 tggacgcatt cgttcataaa cacgccgcac gccattgctg aactccagat ccacgctctc
    55261 gacggtaaac agtcgggaac gggctacagt ttcaacattc agaatggtgg gtttttgtaa
    55321 tgatttgttc atcgtgggat ctatgctgtg aaatcagcgg ttattgtgcg atatcggaca
    55381 cgctttcggc aatgtgaatt gcatgttatt tacatttatg taacttaata aataatcgtc
    55441 ctcaaatcaa attaaaagtc aataggttga aataactcca ggaatttgct gatattccgc
    55501 cttcagaggg tttgctatga tcagcggtta ctgtgatgtg cttaatgatg ctcaagttaa
    55561 gctccacgct tgccgatagc caaccgcaga atcatgtatt gtgtccggtg cgactgacca
    55621 cgcctgacag actaagtaag atggggaaag catgagcacc attgtgattt ttttagctgc
    55681 tttgctggcc tgctcactac ttgcgggatg gctgataaaa gtgcgatcca gacggcgtca
    55741 gctgccctgg accaacgcct tcgcggatgc gcaaacgcgt aaactcacac ctgaagaacg
    55801 tagcgccgtt gaaaattatc ttgagagcct gacgcaggta ttacaggtgc ctggcccaac
    55861 ggaagctagc gcggcaccga tctctctggc gctgaatgcc gaaagcaaca acgtcatgat
    55921 gctgacacac gctatcacac gttacggcat ctctaccgac gatccgaata aatggcgtta
    55981 ctacctcgat tcggtagaag tccacctgcc ccccttctgg gaacagtaca tcaacgatga
    56041 gaataccgtt gaactgattc ataccgattc gctgccgttg gttatttcac tcaacggtca
    56101 tacgctgcag gagtacatgc aggaaacgcg cggctatgcc ttgcaacctg ttccgtccac
    56161 gcaggcgtcg attcgcgggg aagagagtga gcaaatcgag ctactcaata ttcgcaaaga
    56221 aacgcatgaa gaatatgcgc tgagtcgtcc gcgcgggctg cgtgaagcgt tgctgatcgt
    56281 cgcctccttc ctgatgttct ttttctgcct gattaccccg gatgtatttg ttccgtggct
    56341 ggcaggcggc gcattactgc tgctgggcgc aggtctgtgg gggctattcg cgcccccggc
    56401 aaaatcctcc ctgcgagaaa ttcattgtct gcgcggtaca ccccgtcgtt ggggattgtt
    56461 tggcgaaaac gatcaggaac agatcaacaa tatttcactc ggtattatcg acctggtcta
    56521 tcccgcacac tggcagccat acattgctca ggatctcggt caacaaaccg atattgatat
    56581 ctatctcgac cgccacgtag tgcgtcaggg gcgatatctt tcgctgcatg atgaagtaaa
    56641 aaactttccg ttacagcact ggctgcgcag tacgattatc gctgcgggct cgctgctagt
    56701 gctgtttatg ctgttattct ggatcccgct ggatatgccg ctgaaattca ctctctcatg
    56761 gatgaaaggc gcgcagacca ttgaagccac cagcgtaaaa caactggctg acgctggcgt
    56821 gcgggtaggc gatacattgc gtattagcgg tacgggaatg tgtaatattc gcacttccgg
    56881 aacctggagc gcgaaaacca attcaccttt tttaccgttt gactgctcgc agatcatctg
    56941 gaatgacgcc cgctcattac cattaccaga atctgaactg gtcaacaaag cgacggcatt
    57001 gactgaagca gttaatcgcc agctgcaccc taaaccggaa gatgaatctc gcgtcagtgc
    57061 ctcattacgt tcagcgattc aaaaatccgg catggtattg cttgatgatt ttggcgacat
    57121 tgtactgaag acagcggatt tatgttctgc caaagatgac tgtgtgcgac tgaaaaatgc
    57181 gctggtcaat ctcggcaaca gtaaagactg ggacgcgctg gtaaaacgcg ccaacgccgg
    57241 gaagctcgat ggcgtgaatg tgttattacg cccggtgagt gctgaatcgc tggataacct
    57301 ggtggcaacc tccaccgcgc cgtttatcac gcatgaaacg gcgcgagcgg cacaatcact
    57361 aaacagtccg gcccccggcg gattcctgat tgtcagcgac gaaggcagcg attttgttga
    57421 tcagccctgg ccttcggcat cactttacga ctacccgccg caagaacagt ggaacgcttt
    57481 ccagaaactg gcacaaatgc tgatgcatac gccgtttaac gccgaaggta tcgtcacaaa
    57541 aatcttcact gacgccaatg gtacgcagca tattggcctt catccgatcc cggatcgttc
    57601 cggcctgtgg cgctatctca gcaccacatt gctgctactg acgatgctgg gtagcgccat
    57661 ttacaatggc gtacaggcct ggcgtcgtta ccagcgtcat cgcactcgca tgatggagat
    57721 tcaggcctat tatgaaagct gcctgaaccc gcaactgatc accccttcag aaagccttat
    57781 cgaataacac gtttgcgcgg caggttatgc taccctgtcg cgcaaattgc ttcactctgg
    57841 agatttccct catgcatatc aacattgcct ggcaggacgt agataccgtt ctgctggata
    57901 tggacggcac gttgctcgac ctcgccttcg ataactattt ctggcaaaag ctggtgcctg
    57961 aaacatgggg cgcgaaaaac ggggttacgc cacaggaagc gatggaatat atgcgccagc
    58021 aatatcacga cgtacagcat acgctaaact ggtactgtct tgattactgg agtgagcaac
    58081 tgggtctgga tatctgtgcg atgaccaccg agatgggacc gcgcgccgta ctgcgtgaag
    58141 ataccattcc gtttcttgag gcactgaaag ccagcggtaa gcagcgaatt ttgctcacca
    58201 atgctcatcc gcacaacctg gcggtaaaac ttgagcatac gggtctggac gcacaccttg
    58261 atttattact ttccacccac acatttggtt atccgaaaga ggatcagcgg ttatggcatg
    58321 cggtggccga agctacgggt ctgaaagctg aaagaacgct gtttattgat gacagcgaag
    58381 cgattctcga tgctgccgcg caatttggta ttcgttactg cctcggcgtg actaatcctg
    58441 attccgggat tgccgagaaa cagtatcaac gccatccgtc actgaatgac taccgccgcc
    58501 tgatcccctc gctaatgtga aggagacgcc atgaaagaga aacctgctgt tgaggttcga
    58561 ctggataaat ggctatgggc tgcccgtttt tataaaaccc gcgcgctggc ccgtgaaatg
    58621 attgaaggcg gtaaggtgca ttacaacggg cagcgcagca agccgagcaa aatcgtcgag
    58681 ctgaatgcca cgctcactct gcgccaggga aatgacgaac gcacggtgat tgtaaaggcg
    58741 attactgaac agcgtcgccc cgccagcgag gcagccttgc tgtatgaaga gactgcggaa
    58801 agtgtagaga aacgcgaaaa aatggcgatg gcacgtaaac ttaatgcctt aaccatgccg
    58861 cacccggacc gacgcccgga caaaaaagag cgccgcgacc tgttacgatt taaacacggc
    58921 gacagtgaat aactgtcacc tgcaagagag atgattatgc cgcaacatga ccaattacat
    58981 cgctatctgt ttgaaaattt tgccgttcgc ggcgaactgg taaccgtttc ggaaaccctg
    59041 caacagatcc ttgagaacca cgattatccg cagcccgtta aaaacgtgct ggcagaactg
    59101 ctggttgcga ccagcctgtt aaccgctacg ctgaagtttg atggtgatat caccgtacag
    59161 ctgcagggcg acggtccgat gaatctggcg gttattaacg gtaacaataa ccagcaaatg
    59221 cgcggtgtgg cgcgcgtgca gggcgaaatt ccagaaaatg ccgacctgaa aacgctggtc
    59281 ggcaatggtt acgtggtgat caccattacc ccgagcgaag gcgaacgcta tcagggcgta
    59341 gttggtctgg aaggtgatac cctggcggcc tgcctagaag attactttat gcgttctgaa
    59401 cagctgccga cgcgcctgtt tattcgcacc ggcgacgtag acggcaaacc ggctgcaggc
    59461 ggtatgttgt tgcaggtaat gcctgcgcaa aatacccaac aggacgactt tgaccacctg
    59521 gcgacgctaa ccgaaaccat caaaaccgaa gaactgctga ccttaccggc aaacgaagtg
    59581 ttgtggcgtt tgtatcacga agaagaggtg acggtttacg atccgcagga tgtggagttc
    59641 aaatgcacct gctcgcgtga acgttgcgcc gatgcgctga aaacgctgcc tgatgaagaa
    59701 gttgatagca tcctggcgga agatggcgaa attgacatgc attgtgatta ctgcggtaac
    59761 cactatctgt tcaatgcgat ggatattgct gaaatccgca acaacgcgtc tccggcagat
    59821 ccgtaagttc attaatgagt atgtccggca gagagggctc tgccggattt tacgacttaa
    59881 tggcctgatg tgctgcgctt accagcgcct atgaaataaa tcgcaaatta ttttggtcag
    59941 atacagcgtc gtatccgata aaagcgttgt cacttactta agtttatttc cagcgtttca
    60001 ccatctaccg tcagatcgcg ccacgctttc ggcctcgtcc ccagttttcc atccttcgca
    60061 gctgtgagta atttttcttt cgttatttcg ttcggaagta aactcatatc aagcgcgtcc
    60121 cattcttttc tgtctgagtc aaagccatag acaatgactc tgtcatcatt aaaagcaaac
    60181 aggatccgct ccgcctgacc atcactattc agatcttgct cgaccagtac gcaggcgtct
    60241 ttttcaatac aggtcatcac gttatagcga tcctggatta aggccgacca gaatgtcgca
    60301 tccggtttaa cagaaccagg ggcaattaac acattttcgg cgaatacttt ttccgatacc
    60361 tgttcctgaa gatgttgctc tccatctaac gccatcagca gatcccgcgc gcgtttcggg
    60421 tctttcataa acccggcatc gcttttcagc gactcaagcg ccgcacgtcc atagcgaccg
    60481 ctctgttcga gcatgtagat agttacctgg tctggcgtgt ttttgccgct ctgataacgc
    60541 gccatatgac tgttcacgct aatacgcata ctgtccagca ccggcgaatt aagaagcacc
    60601 agtatcacca acaccaataa cgaaaccgca aggttcactt tgccctgaag aacaagggga
    60661 ttttgcccat tacgccagac gatactgaca aaatacccca gcgaccacac cagtaacacc
    60721 agctccgcca gcgcgccctg caagcggtcg acagtccagc cgtactgagc gacccgcagc
    60781 cataaccccc aggtggcgac gaacacatac agcggagcaa ccagcaaagc ggttttaatc
    60841 aggcaacgca acggccccgt ccagggaagt gacgcttttt gcggatcgcg gacaatagcc
    60901 attaagatca attgcaaaaa ggccagcgtt aacagcaacc cggcggcgga gatgtggcga
    60961 gaaatcgcgc tcaggcccgt aaacggcagg gtgatgataa acatcagggt tagcaatgat
    61021 actaacggca gcaaccccgt ggcgattagc gtgaacaact tttgaataga gtcgattaaa
    61081 cgtgactgtg tacgcgcgag gatcaccgcc agtgcggtaa ccagaccaaa tgtaagatac
    61141 ataaaccagt cggttgcaaa aaagagcgta ttgaaaaacg tgatgccaac aagtttaaac
    61201 aactcacccc acagtaaaag caccagccac gtcaggccat tggagagaaa aatcaccagt
    61261 aatataagta cgttatgcca tactgactgg taaaagtagc tatagcggct actgccattg
    61321 cggatacgaa ggctttgttg tatccacggt agcaacaaca ttgccatcaa cagcagatag
    61381 cagccaaaat cccaaatcgc cttttcagct ctccaggggt tcatgccatc agtttgccac
    61441 ttcagccaac cgctcatccc tgtcgtggca ataaacacca gcgccagcca tccccaaagg
    61501 cgcttctgtt taaaagagat cacggagaag agtaaaacgg atgaaaacgc gacggtcgca
    61561 ggcacgccat aaacaatcca actgtgattt tttccggcaa gccaggcgat aagcaggtag
    61621 caaaccagcc cctgaagtaa tccggtagca atcatcccct gaagtaatcc ggtagcaatc
    61681 atcccccagc gtgttgccgg tgaaagttca acgttatcca tgttggttat ccagaatcaa
    61741 aaggtgggtt aattatcgca tccgggcagt agtattttgc ttttttcaga aaataatcaa
    61801 aaaaagttag cgtggtgaat cgatacttta ccggttgaat ttgcatcaat ttcattcagg
    61861 aatgcgattc cactcacaat attcccgcca tataaaccaa gatttaacct tttgagaaca
    61921 ttttccacac ctaaaatgct atttctgcga taatagcaac cgtttcgtga caggaatcac
    61981 ggagtttttt gtcaaatatg aatttctcca gatacgtaaa tctatgagcc ttgtcgcggt
    62041 taacaccccc aaaaagactt tactattcag gcaatacata ttggctaagg agcagtgaaa
    62101 tgcgcgttaa caatggtttg cccccgcaag aactcgaggc ttatggtatc agtgacgtac
    62161 atgatatcgt ttacaaccca agctacgacc tgctgtatca ggaagagctc gatccgagcc
    62221 tgacaggtta tgagcgcggg gtgttaacta atctgggtgc cgttgccgtc gataccggga
    62281 tcttcaccgg tcgttcacca aaagataagt atatcgtccg tgacgatacc actcgcgata
    62341 ctttctggtg ggcagacaaa ggcaaaggta agaacgacaa caaacctctc tctccggaaa
    62401 cctggcagca tctgaaaggc ctggtgacca ggcagctttc cggcaaacgt ctgttcgttg
    62461 tcgacgcttt ctgtggtgcg aacccggata ctcgtctttc cgtccgtttc atcaccgaag
    62521 tggcctggca ggcgcatttt gtcaaaaaca tgtttattcg cccgagcgat gaagagctgg
    62581 caggtttcaa accagacttt atcgttatga acggcgcgaa gtgcactaac ccgcagtgga
    62641 aagaacaggg tctgaactcc gaaaacttcg tggcgtttaa cctgaccgag cgcatgcagc
    62701 tgattggcgg cacctggtac ggcggcgaaa tgaagaaagg gatgttctcg atgatgaact
    62761 acctgctgcc gctgaaaggt atcgcttcta tgcactgctc cgccaacgtt ggcgagaaag
    62821 gcgatgttgc ggtgttcttc ggcctttccg gcaccggtaa aaccaccctt tccaccgacc
    62881 cgaaacgccg cctgattggc gatgacgaac acggctggga cgatgacggc gtgtttaatt
    62941 tcgaaggcgg ctgctacgca aaaactatca agctgtcgaa agaagcggaa cctgaaatct
    63001 acaacgctat ccgtcgtgat gcgctgctgg aaaacgtcac cgtgcgtgaa gatggcacta
    63061 tcgactttga tgatggttca aaaaccgaga acacccgcgt ttcttatcca atctatcaca
    63121 tcgataacat cgttaagccg gtttccaaag cgggccacgc gactaaggtt atcttcctga
    63181 ctgctgatgc tttcggcgtg ttgccgccgg tttctcgcct gactgccgat caaacccagt
    63241 atcacttcct ctctggcttc accgccaaac tggccggtac tgagcgtggc atcaccgaac
    63301 cgacgccaac cttctccgct tgcttcggcg cggcgttcct gtcgctgcac ccgactcagt
    63361 acgcagaagt gctggtgaaa cgtatgcagg cggcgggcgc gcaggcttat ctggttaaca
    63421 ctggctggaa cggcactggc aaacgtatct cgattaaaga tacccgcgcc attatcgacg
    63481 ccatcctcaa cggttcgctg gataatgcag aaaccttcac tctgccgatg tttaacctgg
    63541 cgatcccaac cgaactgccg ggcgtagaca cgaagattct cgatccgcgt aacacctacg
    63601 cttctccgga acagtggcag gaaaaagccg aaaccctggc gaaactgttt atcgacaact
    63661 tcgataaata caccgacacc cctgcgggtg ccgcgctggt agcggctggt ccgaaactgt
    63721 aatgatttga agctggagaa tatctatcca gtatcttata gaaagcaaaa cgggaggcac
    63781 cttcgcctcc cgtttattta cccttctttt gtcgtgccct gcgcccgcgt taccggcact
    63841 ggcagccagg cgcgaatgga aagcccgccc cgctcgctgg tgccaagctc cagcatcccg
    63901 ttatggttat ccacgatacg ctgcacaatt gccagcccta atcccgtgcc gctaatggtg
    63961 cgcgcactgt cgccgcggac aaacggctgg aacaggtgct tacgttgttc cggcgcaatt
    64021 cccggaccgt catcttccac ctggaaccag gcgcgattcg gctccgttcc gctgctgact
    64081 ttgatccagc cattgccata acgggcggcg ttgaccacca tattcgccac cgcgcgtttg
    64141 atcgacagcg ggtgcatttt cacttcaatg ctgccggggt aaagcgcggt ttcaatttcc
    64201 cgctcatagc cactttcggc agcaatcacc tcaccgagca ctgcattaag atccgccatt
    64261 tccatcggca tctcctgccc ggtgcgcagg tagtcgataa actgctcaat gatggcgttg
    64321 cactcttcga tatctttatt gatcgattct gccagatagc catcctgctc gctcatcatc
    64381 tcagtcgcca ggcgaatacg cgtcagcggc gtgcgcaagt cgtgacttac ccccgccatc
    64441 agcagcgtgc ggtcatccgc cagttgctta acaccagccg ccatatggtt aaaggcacgg
    64501 gtaacggaac gcacctccga agcgccatat tcacgcagcg gcggcggaat aatcccttta
    64561 ccaacctgca aggctgcgtg ttcgagatcg accaacggtc ggttctggat acgaataaac
    64621 agccacgccc cgcctatcgc caatagcata atcgccagcg tatagcggaa cagcggagag
    64681 aaatcgccct gatgaatttc ggtcagcggc acgcgtaccc agatattggg cgacagccag
    64741 gttttcagcc agacgacagg cgaacttttg ttgacctcaa cgcgcacttc cgtcgggccg
    64801 cccagttgct gtgccatctg atggcttaag aattcatagt gttgcgccca acgcagacct
    64861 gcctcttcgg cagcctcgtt ggagtagaga gagatcccca gctcacggta gatctcccga
    64921 cggaaagcgg gaggcacaac caactgcgtg ccgtcctcca gttgcagttt gtcggtcatc
    64981 aacatacgca cttcgtacgc gaggacttta ttaaactgct ggaggctcgg caaaatcgcg
    65041 aagttcagca ccaccagata agtcgtcacc aggctggcga acagcaaggt gacgatgagc
    65101 aataacgtac gggcaaatga acttcgtggc gagaagcgca atcgcctcat gctttagagc
    65161 cgtccggtac aaagacgtag cccaggcccc aaacggtctg aatgtaacgc ggatgcgctg
    65221 gatcttcttc taccatgcgg cgcagacgcg aaatctgcac gtcgatggag cgttccattg
    65281 cggaatattc acgaccacgg gcaaggttca tcagcttatc gcgggagagc ggctcacgcg
    65341 gatggctgac cagtgccttc agtaccgcaa actcaccgct ggtgagcggc atcggctcat
    65401 cttcgcggaa catttcgcgc gtaccgaggt taagtttgaa cttaccgaaa gcaattaccg
    65461 cctcttcctg tgacggtgcg cctggcagtt cgttcgcctg acgacgcagc accgcacgaa
    65521 tacgggccag cagttcacgc gggttaaacg gttttggaat gtagtcgtca gcgccaatct
    65581 ccaggcctac gatacggtcc acttcttccc ctttcgccgt caccataatg atcggcatcg
    65641 ggttgctctg actacgaaga cgtcggcaaa tcgacaagcc atcttcacca ggtaacatta
    65701 aatccagtac cataagatgg aaagattcac gagtcagcag gcgatccatc tgttctgcat
    65761 tagcgacgct tcgaacctgg aagccttgtt cggtgagata acgttccagc agcgcacgca
    65821 ggcgcatgtc gtcatcgacc accagaatct tgtagttctc ttgcattgtt tgtactccca
    65881 aaggttcgca acaatttgta agcgtgtatt cttaaaaaag ctcacgttcg tcaccagcta
    65941 aatctggtat gaatttcagc ctaaattgtt acaaagcata ttaaacagca gcttaagtat
    66001 acaatttatt cggcgaaaca ttattgattc tgttgatatg atcacgttat acccaatgtg
    66061 cgcattatca aacagacaaa gggaatcaac gagatgaaaa cgcccctggt tacccgggaa
    66121 gggtatgaaa aactcaaaca agagcttaat tatctctggc gtgaagaacg cccggaggtc
    66181 acaaaaaagg tgacctgggc cgcaagtctg ggcgaccgca gcgaaaatgc tgactatcag
    66241 tataataaaa agcgtctgcg tgaaatcgac cgtcgcgtgc gctatctcac taaatgcctg
    66301 gaaaatctca aaatcgtcga ttactcccct cagcaggaag gcaaagtctt ttttggcgcg
    66361 tgggtggaga ttgaaaacga cgatggcgtg actcaccgtt tccgtattgt cggctacgat
    66421 gaaatttttg gccgtaaaga ttacatctct atcgattccc cgatggcccg cgcattgctg
    66481 aaaaaagaag tcggcgatct ggcggtggtg aatacccctg ccggggaagc gaactggtat
    66541 gttaatgcta tcgagtacgt gaaaccgtaa ggaagagtct taacctcctg ccgatggctg
    66601 gcatttttgc cagccagtcc gtataactat cccctgattt ttgatccgaa aagatgaact
    66661 caaaccatga tgaatgattc gttctgccgc attattgcgg gtgaaattca ggcgcgcccg
    66721 gaacaggttg acgctgccgt tcgcctgctt gacgaaggga ataccgtgcc gtttatcgca
    66781 cgttatcgta aggaaatcac cggcggtctg gatgacacgc agctgcgtaa tctggaaacg
    66841 cgtctgagct atctgcgcga gctggaagag agacgtcagg cgatcctcaa atccatttcc
    66901 gagcaaggca aactcaccga tgatctggcg aaggccatca acgccaccct aagcaaaacc
    66961 gaactcgaag acctctacct gccctacaaa cctaaacgcc gcacccgcgg gcaaatcgcc
    67021 attgaagcag ggcttgagcc gttggctgac ctgctgtgga gcgatccgtc acacacgcca
    67081 gaagtcgccg ctgcacaata tgttgatgcc gataaaggcg tggcagatac caaagccgcg
    67141 ctggatggcg cgcgctatat cctgatggaa cggtttgccg aagatgccac gctgctggcg
    67201 aaagtgcgtg attatctgtg gaagaacgcg catttggttt ctacggtggt gagcggtaaa
    67261 gaagaggaag gggcgaaatt ccgcgactat ttcgatcatc acgaaccgtt gtccacggtg
    67321 ccttctcacc gcgcgctggc gatgttccgt gggcgtaacg aaggcgtact ccagctttcg
    67381 ctgaatgccg atccgcagtt cgatgagccg cccaaagaga gctattgcga gcaaatcatc
    67441 atggatcacc ttggcctgcg cctgaacaat gccccggcgg atagctggcg caaaagcgta
    67501 gtgagctgga cctggcgcat caaggtgctg atgcatctgg aaaccgaact gatgggtacc
    67561 gtgcgcgaac gcgcggaaga tgaagcaatc aacgtctttg cccgtaacct gcacgatctg
    67621 ctgatggcgg cccctgccgg actgcgtgca acgatgggcc tcgatccggg tctgcgtacc
    67681 ggggtaaaag tggcggtggt cgatgccact ggcaaactgg tggcgaccga taccatttac
    67741 ccgcacaccg gacaagccgc aaaagcagcg atgaccgttg ctgccttgtg tgaaaaacat
    67801 aacgttgaac tggtggcgat cggcaacggt acagcttccc gcgaaactga acgtttctat
    67861 ctcgacgtgc agaagcagtt cccgaaagtg accgcacaga aagtgatcgt cagcgaagct
    67921 ggcgcgtcgg tttactcagc ttccgagctg gcagcgcagg agttcccgga tctcgacgtt
    67981 tcgctgcgtg gtgcggtatc tatcgcccgc cgtttgcagg atccgctggc ggagctggtg
    68041 aaaatcgatc cgaaatctat cggcgtaggt cagtatcagc atgacgtcag ccagacgcaa
    68101 ctggcccgca aactggatgc agtagtagaa gactgcgtaa acgccgttgg cgtcgatctc
    68161 aacaccgctt ctgttccgct attaacccgc gtggcgggcc tgacgcgcat gatggcgcaa
    68221 aacatcgtgg cctggcgcga tgagaacggc cagttccaga accgtcagca actgctgaaa
    68281 gtcagccgtc tggggccgaa agccttcgag cagtgcgcgg gcttcctgcg cattaaccac
    68341 ggtgataacc cgctggacgc ctctaccgtt catccggaag cctatccggt ggtggaacgc
    68401 attctggcag caacacagca ggcactgaaa gatctgatgg gtaacagcag cgaactgcgt
    68461 aacctgaaag cgtctgactt tactgatgaa aaattcggtg tgccgacagt aactgacatc
    68521 atcaaagagc tggaaaaacc gggtcgcgat ccgcgtccgg aatttaaaac cgctcagttt
    68581 gccgatggcg tcgagacaat gaacgacctg caaccgggta tgatcctcga aggcgcagtg
    68641 accaacgtca ccaactttgg cgcgtttgtc gatattggcg tgcatcagga cggcctggtt
    68701 cacatctctt cattgtcgaa caagtttgtg gaagatccgc ataccgtggt gaaagcgggc
    68761 gacattgtga aggtgaaagt gctggaagtg gatcttcagc gtaaacgtat cgccctgact
    68821 atgcgtctgg acgagcagcc tggcgaaacc aactctcgtc gcggcggcgg taatgaacgc
    68881 ccgcaaaaca accgcccggc agccaaacca cgcggtcgtg aagcgcagcc tgccggtaat
    68941 agcgcgatga tggatgcgct ggcggcagcg atgggtaaaa aacgttaaac gcccggactg
    69001 gcccctaaac ctgcgcgata aggcgttcac gccgcatctg gcaacgaacg ctttgcctga
    69061 cataaaagtg ccggagaata tctccggcac ttttattcca cagccaaact cacaatatac
    69121 tccgttaata tttatcactt cattaacaac tgaaacctta attaaacatt agccagtccg
    69181 ggtaactcac tattcgaatt atattttcgc tgcgatataa ccttgagcca catcaacatt
    69241 gagtcagatt attattcaaa ccaacattcg cacacatttt aagtattgct gatagaaacc
    69301 attctcatta tcattgtgtt gttgattatt taatctctcc tttgttggca aatcatctgg
    69361 tctcatgtcg cggtcaaacg ccccatgagg tagttatcca gttaatgaga aacaagtagg
    69421 cacctatgca atacactcca gatactgcgt ggaaaatcac tggcttttcc cgtgaaatca
    69481 gcccggcata tcgccaaaaa ctgctttctc taggcatgtt acctggctcc tcttttgatg
    69541 tggtgcgcgt cgctccactc ggcgacccca ttcatatcga aacccgtcgt gtgagcctgg
    69601 tattacgcaa aaaagatctg gccttattag aagtggaagc ggtttcctgt taatacggtg
    69661 ataacaacaa tgaaaaaatt aaccattggc ttaattggta atccaaattc tggcaagaca
    69721 accttattta accagctcac tggcgcacgt cagcgtgtag gtaactgggc tggcgttacc
    69781 gtcgaacgta aagaagggca attctccacc accgatcatc aggtcacgct ggtggacctg
    69841 cccggcacct attctctgac caccatctca tcgcagacct cgctcgatga gcaaatcgcc
    69901 tgtcactaca ttttgagtgg cgacgccgac ctgctgatta acgtggtgga tgcgtctaac
    69961 cttgagcgta acctgtacct gacgctgcaa ctgctggaac tcggcattcc ctgcatcgtg
    70021 gcgctgaaca tgctcgacat tgccgagaag caaaatattc gtattgaaat tgatgctcta
    70081 tcggcacgtc ttggctgccc ggtgatcccg ctggtttcaa cccgtggtcg cggtattgaa
    70141 gcgctcaagc tggcgattga tcgctataaa gctaacgaga atgtggaact ggtgcattac
    70201 gcacagccgc tactcaacga agcagattca ctggcaaaag tgatgccttc cgacatcccg
    70261 ctgaaacaac gtcgctggct gggcctgcaa atgctggaag gcgatatcta cagccgcgcc
    70321 tacgccggtg aagcgtcgca gcatctggat gccaccctcg cccgtctgcg taatgaaatg
    70381 gatgatccgg cgctgcacat tgccgatgcg cgttaccagt gcattgctgc catctgtgat
    70441 gtggtaagca acaccctgac ggcagaaccc agccgtttca ccactgcggt agataaaatc
    70501 gtgctcaacc gtttcctcgg tctgccgatt ttcctctttg tgatgtacct gatgttcctg
    70561 ctggctatca acatcggcgg ggcgttacag ccgctgttta acgtcggctc cgtggcgcta
    70621 tttgtgcatg gtattcaatg gattggctac acgctccact tcccggactg gctgactatc
    70681 ttcctcgccc agggcctggg tggcggtatt aacaccgtgc tgccactggt gccgcagatc
    70741 ggcatgatgt acctgttcct ctccttcctt gaggactccg gctatatggc gcgtgcagcg
    70801 tttgtgatgg accgcctgat gcaggcgctg ggcttgccgg ggaaatcctt tgtgccgctg
    70861 atcgtcggtt tcggttgtaa cgtaccgtcg gtaatgggcg cacgtacgct tgatgcaccg
    70921 cgtgaacgtc tgatgaccat catgatggca ccgtttatgt cctgcggcgc gcgtctggct
    70981 atcttcgcag tattcgcggc tgccttcttc gggcagaacg gtgccctggc ggttttctcg
    71041 ctgtatatgc tcggtatcgt gatggcggtg ctgactggcc tgatgctcaa gtacaccatc
    71101 atgcgcggtg aagcgacgcc gtttgtcatg gagctgccgg tctaccatgt accacacgtt
    71161 aaaagcctga ttatccagac ctggcagcgt ctgaagggct tcgttctgcg tgctggtaaa
    71221 gtgattatca tcgtcagcat tttcctgagc gctttcaaca gcttctcgtt gagcgggaaa
    71281 atcgtcgata acatcaacga ctcggcgctg gcttccgtca gccgggtgat aacccctgtc
    71341 ttcaagccga ttggcgtaca tgaagataac tggcaggcaa cggttggcct gtttacgggc
    71401 gcaatggcaa aagaagtggt ggtgggtacg ctcaacaccc tctacaccgc agaaaatatt
    71461 caggacgaag agttcaatcc ggcggaattc aacctcggtg aagagctgtt cagtgcggta
    71521 gatgaaacct ggcagagcct gaaagacacc ttcagcctta gcgtactgat gaaccccatt
    71581 gaagctagca aaggcaacgg tgaaatgggt accggggcga tgggcgtgat ggatcagaaa
    71641 ttcggtagcg cagctgccgc atacagctac ctgattttcg tcctgctgta tgtaccatgt
    71701 atctcggtga tgggagccat cgcccgtgaa tcaagccgtg gctggatggg cttctccatc
    71761 ctgtgggggc tgaatatcgc ttactcactg gcaacattgt tctatcaggt cgccagctac
    71821 agtcagcacc caacttacag cctggtgtgc attctggcgg taatcctgtt taacatcgta
    71881 gctatcggtc tgctgcgccg cgcgcgtagc cgggtggata tcgaactgct ggcaacccgc
    71941 aagtcggtaa gcagttgctg cgcagccagc accaccggtg attgccatta atggcttcac
    72001 ttattcaggt acgtgatttg ctggcgttac ggggccgtat ggaagcgacc cagataagcc
    72061 agacattgaa cactccgcag ccaatgatta acgccatgct gcaacagctg gaaagcatgg
    72121 gcaaagccgt gcggattcag gaagaacctg acggctgcct ctctggcagt tgtaaaagct
    72181 gcccggaagg caaagcctgt ctgcgcgagt ggtgggcgct gcgttaacca tactccatcg
    72241 cctgttttga aaagcccggt atgcgtttgc atccgggctt ttttgcgtgc ggctttccat
    72301 aaaaatgcaa ttcttgcagc gcaacgtaag ttcctctgaa agcgtctcgc agggatgaaa
    72361 actcgctaat gcacagatgt ggagtggcgc gtagagtcgc ggcattcaaa caacaggtga
    72421 aggaacgcca tgagtaaaaa gcagagttcc accccacatg atgcgctgtt caaactcttt
    72481 ttacgccaac cggagacggc acgcgatttt cttgcgtttc atttaccggc acccattcac
    72541 gcgctctgtg atatgaaaac cctcaagctg gagtcgagga gctttattga tgacgatctg
    72601 cgtgaaagct attccgatgt gctgtggtcg gtgaaaacgg aacaaggacc aggatacatc
    72661 tattgtctga ttgaacatca aagcacctca aacaaactga tcgcatttcg catgatgcgt
    72721 tacgctattg ccgcaatgca aaatcacctt gatgctggat acaaaacgtt gccgatggtg
    72781 gtgccattgt tgttttacca cggtattgaa agcccctatc cctattcgct gtgttggctg
    72841 gattgtttcg ccgatcccaa actggcaagg cagctttatg cctccgcatt tccgctgatt
    72901 gatgtcaccg tcatgcctga tgatgaaatc atgcagcacc gacgtatggc gctgctggag
    72961 ttaattcaaa aacatattcg tcaacgcgat ctgatggggc tggtagagca aatggcctgc
    73021 ttattaagta gtggatacgc taatgacaga caaatcaaag ggctgtttaa ttacatactg
    73081 caaactggcg acgctgtacg ttttaacgat tttatcgacg gcgttgccga acgttcaccg
    73141 aaacacaagg agagtttaat gactattgcg gaaagattgc ggcaggaggg ggaacaatcc
    73201 aaagccctgc atatagccaa aataatgct
//
LOCUS       DAJTYM010000004        70256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTYM010000004 DAJTYM010000000
VERSION     DAJTYM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..785
                     /locus_tag="OO575_000271"
     CDS             <1..785
                     /locus_tag="OO575_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6663372.1"
                     /translation="SLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNN
                     HNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALID
                     ANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVP
                     NRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGI
                     SSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            796..1887
                     /locus_tag="OO575_000272"
     CDS             796..1887
                     /locus_tag="OO575_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6663373.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1930..2790)
                     /gene="rsmI"
                     /locus_tag="OO575_000273"
     CDS             complement(1930..2790)
                     /gene="rsmI"
                     /locus_tag="OO575_000273"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6663374.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2855..4891
                     /gene="lpoA"
                     /locus_tag="OO575_000274"
     CDS             2855..4891
                     /gene="lpoA"
                     /locus_tag="OO575_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6663375.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4849..5244
                     /locus_tag="OO575_000275"
     CDS             4849..5244
                     /locus_tag="OO575_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6663376.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5264..5854
                     /gene="diaA"
                     /locus_tag="OO575_000276"
     CDS             5264..5854
                     /gene="diaA"
                     /locus_tag="OO575_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6663377.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5864..6439
                     /gene="yraP"
                     /locus_tag="OO575_000277"
     CDS             5864..6439
                     /gene="yraP"
                     /locus_tag="OO575_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6663378.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6553..7593)
                     /locus_tag="OO575_000278"
     CDS             complement(6553..7593)
                     /locus_tag="OO575_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6663379.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7666..8301)
                     /locus_tag="OO575_000279"
     CDS             complement(7666..8301)
                     /locus_tag="OO575_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6663380.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8429..8947
                     /gene="yhbO"
                     /locus_tag="OO575_000280"
     CDS             8429..8947
                     /gene="yhbO"
                     /locus_tag="OO575_000280"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6663381.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8927..9370)
                     /locus_tag="OO575_000281"
     CDS             complement(8927..9370)
                     /locus_tag="OO575_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6663382.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9421..9723
                     /gene="yhbQ"
                     /locus_tag="OO575_000282"
     CDS             9421..9723
                     /gene="yhbQ"
                     /locus_tag="OO575_000282"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6663383.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9710..10213)
                     /locus_tag="OO575_000283"
     CDS             complement(9710..10213)
                     /locus_tag="OO575_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6663384.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10207..10731)
                     /locus_tag="OO575_000284"
     CDS             complement(10207..10731)
                     /locus_tag="OO575_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6663385.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10940..11935
                     /locus_tag="OO575_000285"
     CDS             10940..11935
                     /locus_tag="OO575_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6663386.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11944..12822
                     /locus_tag="OO575_000286"
     CDS             11944..12822
                     /locus_tag="OO575_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6663387.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGTVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12903..13910
                     /locus_tag="OO575_000287"
     CDS             12903..13910
                     /locus_tag="OO575_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6663388.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14028..15272)
                     /gene="mtr"
                     /locus_tag="OO575_000288"
     CDS             complement(14028..15272)
                     /gene="mtr"
                     /locus_tag="OO575_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6663389.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15426..17315)
                     /gene="deaD"
                     /locus_tag="OO575_000289"
     CDS             complement(15426..17315)
                     /gene="deaD"
                     /locus_tag="OO575_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6663390.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17308..17388)
                     /gene="yrbN"
                     /locus_tag="OO575_000290"
     CDS             complement(17308..17388)
                     /gene="yrbN"
                     /locus_tag="OO575_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6663391.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17495..18379)
                     /gene="nlpI"
                     /locus_tag="OO575_000291"
     CDS             complement(17495..18379)
                     /gene="nlpI"
                     /locus_tag="OO575_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6663392.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18488..20623)
                     /gene="pnp"
                     /locus_tag="OO575_000292"
     CDS             complement(18488..20623)
                     /gene="pnp"
                     /locus_tag="OO575_000292"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6663393.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20870..21139)
                     /gene="rpsO"
                     /locus_tag="OO575_000293"
     CDS             complement(20870..21139)
                     /gene="rpsO"
                     /locus_tag="OO575_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6663394.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21288..22232)
                     /gene="truB"
                     /locus_tag="OO575_000294"
     CDS             complement(21288..22232)
                     /gene="truB"
                     /locus_tag="OO575_000294"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6663395.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22232..22633)
                     /gene="rbfA"
                     /locus_tag="OO575_000295"
     CDS             complement(22232..22633)
                     /gene="rbfA"
                     /locus_tag="OO575_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6663396.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22797..25445)
                     /gene="infB"
                     /locus_tag="OO575_000296"
     CDS             complement(22797..25445)
                     /gene="infB"
                     /locus_tag="OO575_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6663397.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25470..26957)
                     /gene="nusA"
                     /locus_tag="OO575_000297"
     CDS             complement(25470..26957)
                     /gene="nusA"
                     /locus_tag="OO575_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6663398.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26985..27437)
                     /gene="rimP"
                     /locus_tag="OO575_000298"
     CDS             complement(26985..27437)
                     /gene="rimP"
                     /locus_tag="OO575_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6663399.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27644..27720)
                     /locus_tag="OO575_000299"
     tRNA            complement(27644..27720)
                     /locus_tag="OO575_000299"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27684..27686),aa:Met,seq:cat)
     gene            28068..29411
                     /gene="argG"
                     /locus_tag="OO575_000300"
     CDS             28068..29411
                     /gene="argG"
                     /locus_tag="OO575_000300"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6663400.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29419..31026)
                     /locus_tag="OO575_000301"
                     /pseudo
     CDS             complement(29419..31026)
                     /locus_tag="OO575_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31504..31590)
                     /locus_tag="OO575_000302"
     tRNA            complement(31504..31590)
                     /locus_tag="OO575_000302"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31554..31556),aa:Leu,seq:gag)
     gene            complement(31605..31937)
                     /gene="secG"
                     /locus_tag="OO575_000303"
     CDS             complement(31605..31937)
                     /gene="secG"
                     /locus_tag="OO575_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6663401.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32165..33502)
                     /gene="glmM"
                     /locus_tag="OO575_000304"
     CDS             complement(32165..33502)
                     /gene="glmM"
                     /locus_tag="OO575_000304"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6663402.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33495..34343)
                     /gene="folP"
                     /locus_tag="OO575_000305"
     CDS             complement(33495..34343)
                     /gene="folP"
                     /locus_tag="OO575_000305"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6663403.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34433..36367)
                     /gene="ftsH"
                     /locus_tag="OO575_000306"
     CDS             complement(34433..36367)
                     /gene="ftsH"
                     /locus_tag="OO575_000306"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6663404.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36467..37096)
                     /gene="rlmE"
                     /locus_tag="OO575_000307"
     CDS             complement(36467..37096)
                     /gene="rlmE"
                     /locus_tag="OO575_000307"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6663405.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37222..37515
                     /gene="yhbY"
                     /locus_tag="OO575_000308"
     CDS             37222..37515
                     /gene="yhbY"
                     /locus_tag="OO575_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6663406.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37671..38147)
                     /gene="greA"
                     /locus_tag="OO575_000309"
     CDS             complement(37671..38147)
                     /gene="greA"
                     /locus_tag="OO575_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6663407.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38395..39828
                     /gene="dacB"
                     /locus_tag="OO575_000310"
     CDS             38395..39828
                     /gene="dacB"
                     /locus_tag="OO575_000310"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6663408.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40014..41186)
                     /gene="cgtA"
                     /locus_tag="OO575_000311"
     CDS             complement(40014..41186)
                     /gene="cgtA"
                     /locus_tag="OO575_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6663409.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41202..42167)
                     /locus_tag="OO575_000312"
     CDS             complement(41202..42167)
                     /locus_tag="OO575_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6663410.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42294..42551)
                     /gene="rpmA"
                     /locus_tag="OO575_000313"
     CDS             complement(42294..42551)
                     /gene="rpmA"
                     /locus_tag="OO575_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6663411.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42572..42883)
                     /gene="rplU"
                     /locus_tag="OO575_000314"
     CDS             complement(42572..42883)
                     /gene="rplU"
                     /locus_tag="OO575_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6663412.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43141..44112
                     /gene="ispB"
                     /locus_tag="OO575_000315"
     CDS             43141..44112
                     /gene="ispB"
                     /locus_tag="OO575_000315"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6663413.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44339..44617
                     /gene="sfsB"
                     /locus_tag="OO575_000316"
     CDS             44339..44617
                     /gene="sfsB"
                     /locus_tag="OO575_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6663414.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44665..45924)
                     /gene="murA"
                     /locus_tag="OO575_000317"
     CDS             complement(44665..45924)
                     /gene="murA"
                     /locus_tag="OO575_000317"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6663415.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45979..46233)
                     /gene="ibaG"
                     /locus_tag="OO575_000318"
     CDS             complement(45979..46233)
                     /gene="ibaG"
                     /locus_tag="OO575_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6663416.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46393..46686)
                     /gene="mlaB"
                     /locus_tag="OO575_000319"
     CDS             complement(46393..46686)
                     /gene="mlaB"
                     /locus_tag="OO575_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6663417.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46686..47321)
                     /gene="mlaC"
                     /locus_tag="OO575_000320"
     CDS             complement(46686..47321)
                     /gene="mlaC"
                     /locus_tag="OO575_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6663418.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47340..47891)
                     /gene="mlaD"
                     /locus_tag="OO575_000321"
     CDS             complement(47340..47891)
                     /gene="mlaD"
                     /locus_tag="OO575_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6663419.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47896..48678)
                     /gene="mlaE"
                     /locus_tag="OO575_000322"
     CDS             complement(47896..48678)
                     /gene="mlaE"
                     /locus_tag="OO575_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6663420.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48686..49495)
                     /gene="mlaF"
                     /locus_tag="OO575_000323"
     CDS             complement(48686..49495)
                     /gene="mlaF"
                     /locus_tag="OO575_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6663421.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRRIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49705..50670
                     /locus_tag="OO575_000324"
     CDS             49705..50670
                     /locus_tag="OO575_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6663422.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50684..51670
                     /gene="kdsD"
                     /locus_tag="OO575_000325"
     CDS             50684..51670
                     /gene="kdsD"
                     /locus_tag="OO575_000325"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6663423.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51691..52257
                     /gene="kdsC"
                     /locus_tag="OO575_000326"
     CDS             51691..52257
                     /gene="kdsC"
                     /locus_tag="OO575_000326"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6663424.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52254..52829
                     /gene="lptC"
                     /locus_tag="OO575_000327"
     CDS             52254..52829
                     /gene="lptC"
                     /locus_tag="OO575_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6663425.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52798..53355
                     /gene="lptA"
                     /locus_tag="OO575_000328"
     CDS             52798..53355
                     /gene="lptA"
                     /locus_tag="OO575_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6663426.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53362..54087
                     /gene="lptB"
                     /locus_tag="OO575_000329"
     CDS             53362..54087
                     /gene="lptB"
                     /locus_tag="OO575_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6663427.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54135..55568
                     /gene="rpoN"
                     /locus_tag="OO575_000330"
     CDS             54135..55568
                     /gene="rpoN"
                     /locus_tag="OO575_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6663428.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55591..55878
                     /gene="hpf"
                     /locus_tag="OO575_000331"
     CDS             55591..55878
                     /gene="hpf"
                     /locus_tag="OO575_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6663429.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55996..56487
                     /gene="ptsN"
                     /locus_tag="OO575_000332"
     CDS             55996..56487
                     /gene="ptsN"
                     /locus_tag="OO575_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6663430.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56533..57387
                     /gene="rapZ"
                     /locus_tag="OO575_000333"
     CDS             56533..57387
                     /gene="rapZ"
                     /locus_tag="OO575_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6663431.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57384..57656
                     /gene="npr"
                     /locus_tag="OO575_000334"
     CDS             57384..57656
                     /gene="npr"
                     /locus_tag="OO575_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6663432.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57869..58501
                     /gene="yrbL"
                     /locus_tag="OO575_000335"
     CDS             57869..58501
                     /gene="yrbL"
                     /locus_tag="OO575_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6663433.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58498..59225)
                     /gene="mtgA"
                     /locus_tag="OO575_000336"
                     /pseudo
     CDS             complement(58498..59225)
                     /gene="mtgA"
                     /locus_tag="OO575_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59222..59875)
                     /gene="elbB"
                     /locus_tag="OO575_000337"
     CDS             complement(59222..59875)
                     /gene="elbB"
                     /locus_tag="OO575_000337"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6663434.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60105..62441)
                     /gene="arcB"
                     /locus_tag="OO575_000338"
     CDS             complement(60105..62441)
                     /gene="arcB"
                     /locus_tag="OO575_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6663435.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62537..63466)
                     /locus_tag="OO575_000339"
     CDS             complement(62537..63466)
                     /locus_tag="OO575_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6663436.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64141..68601
                     /gene="gltB"
                     /locus_tag="OO575_000340"
     CDS             64141..68601
                     /gene="gltB"
                     /locus_tag="OO575_000340"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6663437.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68614..70032
                     /gene="gltD"
                     /locus_tag="OO575_000341"
     CDS             68614..70032
                     /gene="gltD"
                     /locus_tag="OO575_000341"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6663438.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
BASE COUNT        17193 a        19078 c        17448 g        16537 t
ORIGIN      
        1 gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa catgaaatgc
       61 gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa caaagtctgg
      121 atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct tatggaacct
      181 tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc gttaccggcg
      241 gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac gcttttgcgc
      301 ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg attgctactg
      361 atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac cgtctctcgg
      421 tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag tttgtggtgc
      481 ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat cgcgtacttg
      541 ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc agcaacgatg
      601 atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct gggatatcga
      661 gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa tgtcagtttg
      721 cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca gcgcagtgcc
      781 attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt gatatccaca
      841 tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg tgcgacaaat
      901 tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac aggacgggag
      961 tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg ctctgcgggt
     1021 aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg acatcagaca
     1081 ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc aaacgacatt
     1141 ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca gataaaaagc
     1201 agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac gtctcgttat
     1261 ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa gccatttctc
     1321 ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg ggtcgatgga
     1381 atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat tcagggaaat
     1441 ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt taattttgga
     1501 ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt tactccagta
     1561 gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc gttgcaaatg
     1621 cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag gcgaagaagt
     1681 tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg agttgcaaat
     1741 attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac cgtcaatatg
     1801 cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt tcagggcaca
     1861 gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta acgcaacgct
     1921 tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt tcttcacgcc
     1981 atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct gtagcagcgc
     2041 cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac cttcgacaat
     2101 cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca gcagctcgcc
     2161 aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca gaaccacgta
     2221 gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac ggtgggtaga
     2281 ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat cacggcggcc
     2341 ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta aacccgcggc
     2401 gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa tccccgcttc
     2461 acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc cggcatcgga
     2521 aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg ctttttgttg
     2581 ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt gcagcaataa
     2641 accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata cctctaacgt
     2701 acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa gctggccctg
     2761 agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga tttaatattg
     2821 agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat tttctcgttt
     2881 gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg gttgtggcac
     2941 ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg attctgcctt
     3001 ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc aattactcgc
     3061 cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt ttaaccaact
     3121 accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg cagagattaa
     3181 actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca cacctgccga
     3241 tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca gccaggggcg
     3301 tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg gcgcgaaaga
     3361 aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc aggaacaggc
     3421 gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg atctgcagcg
     3481 cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg ccgactggca
     3541 gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg ttaacgtaaa
     3601 agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga atggccaggc
     3661 agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata tcggcactca
     3721 gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac aacctcagcc
     3781 gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc tgaccggtga
     3841 tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg cagcggtaag
     3901 cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac aaccacttag
     3961 ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc cgttgctgaa
     4021 aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg cactgaacca
     4081 gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac cggaagacga
     4141 agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc tggtgctgat
     4201 cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt ggcagaaact
     4261 gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac gcgcgggtgt
     4321 taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag cgacaaccga
     4381 ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg accagttcac
     4441 caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg aaatcgcttt
     4501 tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc tgtacgccag
     4561 ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg aaggcttgca
     4621 gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc aggcgctcag
     4681 cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg atgcctggtc
     4741 gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca acggtaatac
     4801 cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat ggctacagta
     4861 ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga tgcgtgggaa
     4921 gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc taacgtgaac
     4981 gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt ttttgtcgag
     5041 gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac ccgcagcaaa
     5101 caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg gagttttgat
     5161 actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga gtggattaag
     5221 gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag aaagaattaa
     5281 agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc ttccggatgc
     5341 catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca aaatcctctg
     5401 ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca tgatcaaccg
     5461 tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata atgttgtctt
     5521 aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg tgcgggcgct
     5581 gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc gcgatattgt
     5641 taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga ccggctatga
     5701 cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca ttccttcgca
     5761 tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt gcgatctgat
     5821 cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg cattatcgcc
     5881 aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg ccgtagtggg
     5941 taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca cccaggtgga
     6001 cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac agattaagaa
     6061 agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg ggcagtcacc
     6121 aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg gtgccaacga
     6181 agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat ctaacgatac
     6241 gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga aatcgtccaa
     6301 cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga ctgaacgtga
     6361 agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg ttaccacagc
     6421 gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg ccgcgtctta
     6481 tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc atccgacatg
     6541 ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag acactgccac
     6601 catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca taatcagtga
     6661 cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg ttcccatacc
     6721 tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga caaacaagca
     6781 tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac catcggcatg
     6841 ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt aaaccgggat
     6901 cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac cgctctgtgc
     6961 ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca cccatttttg
     7021 caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa tcgccgcaaa
     7081 accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca ccggattgcc
     7141 catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc ccgccgcgac
     7201 cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc ccagcagcgt
     7261 gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga tcagcacctg
     7321 aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga ggaaatagat
     7381 catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa ggatagattt
     7441 accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt gccatttcac
     7501 ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc actgaatggg
     7561 cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata agttatgata
     7621 attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt cagctctttt
     7681 gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca tcgcctctgc
     7741 cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg gtaacaaacg
     7801 gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc ccagtaacat
     7861 cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt cttccatctc
     7921 ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag cactgaccac
     7981 cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta ccagcgtgta
     8041 atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc cgagacaaca
     8101 aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt gcggatcatg
     8161 gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag caatggcgtt
     8221 aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc ccgttgcgcc
     8281 tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt gcgctccccg
     8341 gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg aaaattcgcc
     8401 tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt taatcactga
     8461 tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag ccggacacga
     8521 agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag gagaagccag
     8581 cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg ccctgttgct
     8641 accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca cctttacccg
     8701 tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc agctgctgat
     8761 cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca ttattgatgt
     8821 taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag atcagctggt
     8881 taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac gcctgctcgg
     8941 tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg tcggtgaatt
     9001 tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca accggaaagc
     9061 gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg cggatctcac
     9121 ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt accgttccgg
     9181 caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc gtcaaaatgt
     9241 agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag agttcccctt
     9301 cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg atggcgatga
     9361 gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc gatgcagacg
     9421 atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta taccgggatc
     9481 accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc gaaagcactg
     9541 cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg ttcgctggca
     9601 ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg tctggtagcg
     9661 gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat taaaagcgat
     9721 tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca tccgctaaac
     9781 gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg tgatgcgccg
     9841 ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc actgcggcat
     9901 agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc agcccctgtc
     9961 cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg tcttcgccct
    10021 gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca tctgtcgcca
    10081 ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc gcttccgcat
    10141 cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg ggaatttcta
    10201 ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg ttttcattcc
    10261 cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg gcatttgctc
    10321 cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat cgccttcaat
    10381 caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg caatcatcag
    10441 cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc tgaccaccag
    10501 tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat gaatacttaa
    10561 ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga attgccagct
    10621 taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta ccggtacact
    10681 caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca cgagttttac
    10741 tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag cggtataaat
    10801 caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc ttaaatcaaa
    10861 aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg ttttgatagc
    10921 gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc cggcgcttaa
    10981 ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg ataccaatgc
    11041 ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg tgagttttgt
    11101 ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc cggacggtta
    11161 cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg cgctgatcct
    11221 cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg aacgtcatgt
    11281 gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc gccattttga
    11341 cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac agctggcacg
    11401 ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta tgtcggaagg
    11461 ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg gtgcgtgttc
    11521 tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc gcctgaacga
    11581 agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc tgtgtaaagg
    11641 gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca gtctcaatac
    11701 cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa ttgaaggccg
    11761 ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg ctatcgaccg
    11821 ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga cgctcgggtc
    11881 gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc agtgagaaga
    11941 gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac gctggaagat
    12001 ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga agcggtatgc
    12061 agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc gctcgccgga
    12121 agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc tgaactgggc
    12181 gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga tctcggcgtg
    12241 gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct gaactgctac
    12301 aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg tatgccggtg
    12361 gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct gggcattcgc
    12421 aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta ctccgcccgc
    12481 tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg ctgcattaag
    12541 tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt actcaatggc
    12601 attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc catgcaggga
    12661 ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat gctcgacgcc
    12721 ttccgcgcta atgaaaatgg cactgtgcca ctgccgttga cggcaaacag cgactgtaac
    12781 ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag ctcactttgt
    12841 taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca aagtgagctg
    12901 ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata cccgaaggct
    12961 cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct gaaaagcgcg
    13021 gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc agtgctgcaa
    13081 catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg gggtctggcg
    13141 gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc acactcaata
    13201 cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt gaccaacgga
    13261 caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc cgcgatgtcg
    13321 cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc ccggtaccag
    13381 gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt gcacaactgg
    13441 cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat atgttgttcc
    13501 aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa aaaccatacg
    13561 cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa tttctgttta
    13621 cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa ctgccgccgc
    13681 cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg cagcaggcgc
    13741 tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag tcgatcctgc
    13801 gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac caggcgcgcc
    13861 tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag tgtgtcttaa
    13921 cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata agcgaagcgc
    13981 atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta ctgatacacc
    14041 ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc aaacaccaga
    14101 atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc aaagcgttta
    14161 cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc cgctaaacca
    14221 gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac aacaggcggg
    14281 gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa accaaacaga
    14341 tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc taccgcaaag
    14401 tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc gcttaatgct
    14461 tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc cggacgaggg
    14521 atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat cagcgtaccg
    14581 tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat caggcttggc
    14641 acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag caaatacggc
    14701 gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac atgccccagc
    14761 aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat cgccgtcatg
    14821 cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc aaaaccaaaa
    14881 cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg cagaatcgaa
    14941 ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat ggaaatgccg
    15001 ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa actcgaaccg
    15061 attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat acagaaccag
    15121 gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac cactggcaga
    15181 gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc aagcagcgac
    15241 ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg aaaaatagtg
    15301 cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac agaaaaaaag
    15361 ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc gctgttcagc
    15421 gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg acgcggtgcg
    15481 cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc ttcacggcgt
    15541 tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg accaccgcca
    15601 cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt catcggcttg
    15661 ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc tttcggcagt
    15721 tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct gctgatgtcg
    15781 ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc gcggcccact
    15841 tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg acgatcttca
    15901 cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc acggtcacgg
    15961 aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg ttcaccctgt
    16021 gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc ttctgcagtc
    16081 ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc cagctgctgc
    16141 tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc gttcggcagt
    16201 tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag acgacgctcg
    16261 cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt acgaccgata
    16321 cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag gctgatacgc
    16381 tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag acgaccgtct
    16441 ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt cagcgcggcg
    16501 ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt tttggtacga
    16561 acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag tgcttcgttt
    16621 ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt agtcacgctg
    16681 gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg acgaatcgct
    16741 tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg cgccataatg
    16801 gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc cagaaccaga
    16861 ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag acggcccgga
    16921 gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc ataacgctgg
    16981 ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc cgtcattgct
    17041 tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg tggtgctttc
    17101 agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt tccgctcccc
    17161 gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca ctctgcctga
    17221 attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg agccttcagg
    17281 cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct caaaattaat
    17341 ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg aaaagtgtga
    17401 accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa ggtgatggca
    17461 atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat tctgccaggt
    17521 catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc tcaacaaagt
    17581 tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc aaatccccca
    17641 gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca gcgagcgagg
    17701 tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta atgttgccca
    17761 ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg aagtgctgtt
    17821 tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa agccacagac
    17881 tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct tgcgctaact
    17941 tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag ttgtaagttg
    18001 gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg cctgcctgcg
    18061 ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt gccagcgctt
    18121 gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac actccgcgct
    18181 catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg atttgttcca
    18241 tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg aggacttcac
    18301 ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca acgaaacacc
    18361 agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa cgtcctgttc
    18421 ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg gagggcaaat
    18481 ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca ggttgagact
    18541 gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact tccagaactt
    18601 tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg cgtttgtcag
    18661 cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca aatgcgccaa
    18721 agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt tctgcagtga
    18781 tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct gcaatcttca
    18841 cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg gcacggataa
    18901 cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg atggtatgga
    18961 tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt tccattacgc
    19021 ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg atctctttgg
    19081 tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg gaacccgcaa
    19141 ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac agtacaacgt
    19201 agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct ttgatcggca
    19261 cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa gaggaaccgt
    19321 tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc atatccggca
    19381 tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga cgcttcggag
    19441 aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg aacaggaagg
    19501 tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca gtacccagcg
    19561 ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg tgagtacgcg
    19621 gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga ccgtcgatac
    19681 gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg tgcagaattt
    19741 cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg atggtttcag
    19801 atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg taagcatcgc
    19861 tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt accggctccg
    19921 gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg ttctgaataa
    19981 caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct tcgctcagca
    20041 gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca accagatcca
    20101 gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca ttgatgtaac
    20161 ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac agtgctgcgg
    20221 aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa accacggtgg
    20281 cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc gggcggtcaa
    20341 tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg aagaagctac
    20401 ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt gggaagaagt
    20461 cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg gtgtcatcca
    20521 tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc agagtcacgg
    20581 tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata tcctttctct
    20641 ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc atcctcgcga
    20701 ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc gcgcgaacct
    20761 ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt gctgccgtca
    20821 gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat tagcgacgca
    20881 gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc aggtagtcga
    20941 gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg tgatcttttt
    21001 tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt gctacctgaa
    21061 cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt ttagctgttg
    21121 cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg acgccgatct
    21181 ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg cgacgttaag
    21241 cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta cgccgggtat
    21301 tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc cataccgata
    21361 aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc accggatgta
    21421 cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt caccaccggg
    21481 tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc cgcagccgga
    21541 atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt cacaatccgt
    21601 tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc gccacaaccg
    21661 agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga gcagtgaatt
    21721 tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac ggtaatcggg
    21781 cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt tttaccctga
    21841 tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa agtatccagc
    21901 gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat ttgtccgtcg
    21961 gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg tttgtcggag
    22021 tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa catgccagtt
    22081 gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat ccgtttcact
    22141 ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa aacgccgtta
    22201 atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg tccgggttaa
    22261 cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg cgcatccctt
    22321 caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg cgcatcgctt
    22381 tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg cccgctttaa
    22441 ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac gccaggtcgc
    22501 gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct ttaatttcac
    22561 gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc gggcgaccaa
    22621 attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc tttttaaaat
    22681 taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc gtcacatcag
    22741 gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta aaaaccttaa
    22801 gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc agtgcggacg
    22861 tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc gttaacgtca
    22921 tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt gtcacgcaga
    22981 acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc tgcgatggca
    23041 ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat ctgctgtttc
    23101 agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag gttatagatg
    23161 acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc agaggcatca
    23221 gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt ggcgtcggtt
    23281 tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc agtagacagt
    23341 ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt caggacgatg
    23401 ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag tttagatttc
    23461 tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc aacttcacgc
    23521 gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac gccggacagg
    23581 ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc cagttcgtta
    23641 cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc cttgtgcaga
    23701 gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag gaaggattcg
    23761 ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac ttccgcctgc
    23821 agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga cacatgtacg
    23881 aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag ttcgttctta
    23941 acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac caccggaacc
    24001 tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc gtcgtcggca
    24061 gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat tgaagtaaac
    24121 gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc aacgtggtat
    24181 gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt tgaacgaatg
    24241 tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac aaccggcgcg
    24301 cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc cagctcgttt
    24361 tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc ggtttcctga
    24421 tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt gatgacctga
    24481 gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc gccgatcaca
    24541 acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga agaaccttta
    24601 cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc agcttttgat
    24661 tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc acggccacgg
    24721 ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg agaagtagtg
    24781 acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt gttttcttcc
    24841 gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt acgacgcgct
    24901 tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc tttttcagcc
    24961 tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc cgcagcttca
    25021 cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg ttcagctttt
    25081 tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc ttccgctgca
    25141 aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt
    25201 tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg tgttttacgt
    25261 tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat caaagtctgt
    25321 ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc agcaaattgc
    25381 tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat cgttacatct
    25441 gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca gcaaatatta
    25501 cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat atcagccaga
    25561 tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc ggccagtttg
    25621 aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg tttgttatca
    25681 ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc acgctcgcgc
    25741 agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt catcggcaca
    25801 taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc gaagtcttcg
    25861 tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta
    25921 gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg cgaagccaga
    25981 cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc aacggcgata
    26041 tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg tgccattgcg
    26101 ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg ctcgccgccc
    26161 agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc acctaccgga
    26221 tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg gtcgcgagcc
    26281 gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg gaacagttcg
    26341 atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc ttccgggcga
    26401 acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg cggcagcata
    26461 tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat gttgtcgcgg
    26521 tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc
    26581 accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc cgtctgggtg
    26641 gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc caagttcagg
    26701 ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg ggtgacttca
    26761 tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc gatctgtaca
    26821 cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc gctttccaat
    26881 gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc aactacagcc
    26941 aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa gtggggaacc
    27001 aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc gacggtaact
    27061 gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg gttttgtacc
    27121 gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg ttcagccgtg
    27181 aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac ggtgatggga
    27241 tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc atcaacattg
    27301 atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg aataaattcg
    27361 atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc tgttaatttt
    27421 tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa gcccagttat
    27481 tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact gaaccctata
    27541 accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca ctacgaaggc
    27601 cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc gggggccgga
    27661 tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct ccaccccgcg
    27721 cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg ctgggatttg
    27781 gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc acattttgtc
    27841 aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt tgaatacttt
    27901 tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta aactcttcct
    27961 gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga
    28021 gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg acgacgattc
    28081 tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt ctggacacca
    28141 gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat actgcaaacc
    28201 tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg gaatacggcg
    28261 cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt attgccgcta
    28321 ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac acgacgccgc
    28381 tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat ggcgtgaata
    28441 tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat cgttatggtc
    28501 tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac tttattgatg
    28561 aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc gactacaaaa
    28621 tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg catgaagcga
    28681 aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg ggcgtgaaat
    28741 tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt gagcaaggtc
    28801 atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg ctggaagcta
    28861 accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac cgtatcatcg
    28921 aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac attgcgtatg
    28981 aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac gcgcatggtc
    29041 gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg ctgatgctgc
    29101 gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc ctggagctgc
    29161 gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc tacaagccag
    29221 agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt attggtcaat
    29281 tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt tatgccaaaa
    29341 ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat atggaaaaca
    29401 aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt gcttatcctc
    29461 tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat ccacaacccg
    29521 tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt ccggctttgt
    29581 tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga tattgttttc
    29641 cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca tcgggacatg
    29701 atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct tcgtagggtc
    29761 acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt ttaataattc
    29821 aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc aggcatcctg
    29881 atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag ccggttcatg
    29941 gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct gtaatgcttg
    30001 cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt ccatggcgcg
    30061 catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca
    30121 gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt aattatgaat
    30181 gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg cagtgtaagg
    30241 tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt caacttgcgg
    30301 tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag actcccccac
    30361 aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat acggcaccgt
    30421 gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat agttgagatt
    30481 gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga acgctttttt
    30541 attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg aaatgacaat
    30601 cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt ttattattac
    30661 tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat acatccccag
    30721 cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc catcattgaa
    30781 agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg cagtcagtaa
    30841 cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt taacaaccag
    30901 gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag aggcgactaa
    30961 gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt taaaatcttc
    31021 tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat ataaacaatt
    31081 atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat atcagaccga
    31141 aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt aattaaataa
    31201 cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt atactgacag
    31261 gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac tggagttgac
    31321 acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg cgtaccggca
    31381 ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag atacctgaca
    31441 accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct cttttctgga
    31501 atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac cacctcaagg
    31561 tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt cgggatatcg
    31621 ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg tgcactcaga
    31681 ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag caccagactg
    31741 atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt accagaacca
    31801 cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat atcagcgcct
    31861 ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag gaaaactact
    31921 aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac caatgcttca
    31981 accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata ctaaccaaaa
    32041 cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa aaaacagcaa
    32101 aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc agttatctaa
    32161 cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt
    32221 cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta cgcagcaaca
    32281 cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta actgactcat
    32341 gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca
    32401 ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc gccgccagca
    32461 cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg atcacatgac
    32521 cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt acgtagcggt
    32581 cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt tcaagcccca
    32641 tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga agaccttcac
    32701 gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg tcaaccataa
    32761 tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca gccagcacac
    32821 gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt acaccgtttg
    32881 gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt
    32941 gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg ctaagttcgt
    33001 tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg atacggctgg
    33061 ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg
    33121 cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta atcccattat
    33181 cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg aaggtacgcg
    33241 tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg
    33301 ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc gtgtctttac
    33361 caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc caacccagct
    33421 taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc ccatcggtac
    33481 cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt ggcttccacc
    33541 acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc
    33601 attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg ccccacgttc
    33661 agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag gttgaaatgg
    33721 tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt accgaaaccg
    33781 aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca acgtgctatt
    33841 tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg agcttcctgc
    33901 atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt ttctgcagcc
    33961 gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg agcgccaact
    34021 ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca gacttcgaag
    34081 cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc aacgctaact
    34141 tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg
    34201 atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc accatccgaa
    34261 aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag gtccagtgaa
    34321 gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa gaaaaaaccc
    34381 cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga acttacttgt
    34441 cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca tcaaccggac
    34501 gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt tcttcccagc
    34561 ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag
    34621 tctcatattt catgagagca tctttcatcg catgcagaat atccatattg tcggtcagaa
    34681 gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac
    34741 gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg aatacttcac
    34801 cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac tgagtcacca
    34861 tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta gaaacatgtt
    34921 ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa atctggcttt
    34981 ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa gtcacaccca
    35041 gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc ggcaccaggc
    35101 gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc gcttccgtca
    35161 tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc tcgaactcaa
    35221 ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt
    35281 tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc gcgtcgatat
    35341 cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat
    35401 ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg gccgggtcga
    35461 gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct tcgaagccat
    35521 ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca ccgcccagac
    35581 cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg cacggtgccg
    35641 ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg acgaacattt
    35701 ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct gcaatcgctt
    35761 tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct ttcgggatct
    35821 taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt tcagcaactt
    35881 cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg
    35941 tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg ccgccctgca
    36001 tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac caggagatga
    36061 agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg acgttcttgg
    36121 tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta taacggttac
    36181 tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga acctggtcgt
    36241 tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac tcgctgggcc
    36301 caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt attaggtttt
    36361 tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac agcgtcagga
    36421 tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag ggtttacgcc
    36481 cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc ttacgaactt
    36541 tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct tcgccctgga
    36601 acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt tctagcgcca
    36661 gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc atgtttggtg
    36721 ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc agtgctttca
    36781 tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata ggatccatag
    36841 gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc acatattgtg
    36901 accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta aagagtttgt
    36961 cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc tttttctgtg
    37021 cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag ctggcagaac
    37081 gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca attgcgtaaa
    37141 tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc gttttcaatc
    37201 ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca cctgaaaggt
    37261 ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac cgaaggggtg
    37321 ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa aatcgccacc
    37381 gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt
    37441 aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga acgtaaaatc
    37501 tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa acgaggggtt
    37561 ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc cgcagagcgg
    37621 cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc ttacaggtat
    37681 tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac cacaacatca
    37741 tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat caggttttgt
    37801 ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc agaatccaga
    37861 ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat tttggtgaca
    37921 tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc ttcgcagaag
    37981 ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc
    38041 gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa atccagctct
    38101 tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg ttatacctct
    38161 tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg cccggcataa
    38221 ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc aggtcagcta
    38281 caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc gtttacttta
    38341 tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga gattatgcga
    38401 ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca ggccgcgaat
    38461 gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat ggtgcaaaaa
    38521 gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct gcccgccagt
    38581 acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga ttttcgtttc
    38641 accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg
    38701 gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact
    38761 ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac ctccattttc
    38821 gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg ctttagcgct
    38881 ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta cagtgcccca
    38941 aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac gatgttcagc
    39001 caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact ggatgtggtg
    39061 ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc tgagccgctc
    39121 ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct gaaagatgag
    39181 ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca ggttaacgaa
    39241 cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct taagattatg
    39301 ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg ccatgcgcgc
    39361 ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat cctgcgccag
    39421 caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct ttcgcggcat
    39481 aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca acacgacaat
    39541 gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt gcagtaccgt
    39601 gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg ttcgttgcag
    39661 ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat ggcatttgta
    39721 caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg tattccgtta
    39781 gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc aaaaagaaac
    39841 cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta tgccggatgc
    39901 ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg caatcatctt
    39961 gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag tgattaacgc
    40021 ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc ccagtcttca
    40081 tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca catgaattcg
    40141 actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat gataaaggtc
    40201 atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat cagataatat
    40261 ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc ttctgcctta
    40321 tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag atcctggctg
    40381 tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt gccgtcaatc
    40441 ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt caggaagcga
    40501 atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc ggcaacaacg
    40561 aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt ggtaaacgga
    40621 taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga tttacccgcg
    40681 tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag cagctcacgc
    40741 ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga cgatttgaaa
    40801 cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg accgtgttta
    40861 gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc taccggcact
    40921 ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc gttctgaccg
    40981 cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag gttctcgtcg
    41041 gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc gcctttcgga
    41101 atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc aacgaccaga
    41161 atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc tgcgcggggt
    41221 tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc aatggcggaa
    41281 tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt taacatcggt
    41341 ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag cgtaaacagt
    41401 ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat cgcttccgcc
    41461 aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc gagctgccag
    41521 tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat tgtacataaa
    41581 gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac cttttgcgcc
    41641 acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc ccaggtgtaa
    41701 tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac cagaccgctc
    41761 aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag gataaatacg
    41821 ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc ggtcggactc
    41881 aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc ggtcgccact
    41941 gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa cctcttcttc
    42001 accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa cacgattgtc
    42061 ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc ccagcaaatt
    42121 gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc gtattccctg
    42181 caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta tcgaatgtaa
    42241 aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa aacttattca
    42301 gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt cactttaccg
    42361 tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc agcgtggaat
    42421 ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc accgaaacgc
    42481 ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc gccagccttt
    42541 ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta attttcacat
    42601 cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga cgaaacttaa
    42661 cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt acgccgccat
    42721 caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc acttcagcga
    42781 actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc tgaccttcgc
    42841 ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac tccgcttccg
    42901 cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg cgcgaatatt
    42961 acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa aaacacaact
    43021 tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat tcctaaccat
    43081 aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg
    43141 atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt taatgcggca
    43201 atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc
    43261 agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg agctgttggc
    43321 tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca cacggcgact
    43381 ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc taccgccaac
    43441 gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc
    43501 cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga agctgtaaac
    43561 gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga catcactgaa
    43621 gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag
    43681 tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca ggattatggg
    43741 cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa tgccgatggc
    43801 gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg
    43861 ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac cgccatcgaa
    43921 caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc ttgtggatct
    43981 cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc agcgttacag
    44041 gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa
    44101 cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc cagtaaattc
    44161 cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat ctttatatat
    44221 tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca ggagtatagt
    44281 gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt gaggaaagat
    44341 ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc gcaagaaagg
    44401 aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc
    44461 attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg gaactgaccc
    44521 ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta tcgacagaac
    44581 gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga
    44641 acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat ttgcacctaa
    44701 agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa tacgatcaac
    44761 caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg cacgcagatc
    44821 ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa cggtattgct
    44881 ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa agcggttttc
    44941 aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca gcgtgaactg
    45001 ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa cagccttcgg
    45061 acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt ccgctccagc
    45121 atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac ggcagataat
    45181 tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac gatccggcag
    45241 cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac gatcggtgcc
    45301 ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga cgatttccgg
    45361 ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag cacacatgat
    45421 ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt tcagacgacc
    45481 atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta attgttcgag
    45541 gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag gtagtgaaac
    45601 ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag cacgcatggt
    45661 tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa tatgcacaga
    45721 accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg tatcgacgtc
    45781 tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg cggcaaaaag
    45841 aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga gcttcgttgg
    45901 cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca tatccgctac
    45961 cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg
    46021 ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt ccatcagcgg
    46081 gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact cacccacggc
    46141 aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg agagagcgtt
    46201 catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca gttaataaac
    46261 cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc ctcgctgata
    46321 aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac gccatatccg
    46381 gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt
    46441 atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt tcgccagatc
    46501 gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac ggctaagatc
    46561 gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta gcagaacgtc
    46621 ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc tcagtgactc
    46681 gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt cagttgcgca
    46741 gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg tttggtggtg
    46801 atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt ctgggagttt
    46861 ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat aatggtaacg
    46921 cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg ataggtttga
    46981 ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca
    47041 aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag caccagcgca
    47101 ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt acgcagataa
    47161 tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc
    47221 gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc actcagaggt
    47281 gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat
    47341 tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc agctggcgca
    47401 tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc aatgagatct
    47461 tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag gatagcagtc
    47521 cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc cagcaggccg
    47581 gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg ttcaatttcc
    47641 agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac ccggcccaca
    47701 acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc
    47761 gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa
    47821 acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat ttcatttttt
    47881 ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat ccagccccag
    47941 aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc cggcagacgt
    48001 cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg cgaacaccac
    48061 gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt gcattgccga
    48121 ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc cgccccagat
    48181 ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag cccagaaacg
    48241 gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg agagttgctc
    48301 tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc cggcaaacaa
    48361 cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca gcatacccag
    48421 actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca acaccattcc
    48481 gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat tatagagctg
    48541 gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa
    48601 cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc cgagcgacgc
    48661 cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg tgataatcgc
    48721 cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac tgacgtacgc
    48781 gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt tttttgtccg
    48841 ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga gaaaccacca
    48901 cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt acgcccatgg
    48961 tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc tccagcgcaa
    49021 tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa ggcattagtt
    49081 tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta tgcaacaatg
    49141 gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat acgttcatat
    49201 cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact gtatacaggc
    49261 gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca tgatctggtg
    49321 cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat ggccccatga
    49381 tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg cggcgattgc
    49441 cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc tccataattc
    49501 acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg aacccaacat
    49561 atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta ctttttagcc
    49621 gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc ggaccggcga
    49681 gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat tgttggttta
    49741 cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct ttgccgaacc
    49801 tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac atcgttacca
    49861 gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc cgttggtaca
    49921 gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc gctggttcgt
    49981 ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt gttggtcagc
    50041 gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg tatctttctc
    50101 ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca ggggactgac
    50161 agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt
    50221 ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt ggttgataac
    50281 gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct gacggcaatt
    50341 gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa
    50401 aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt catcgtgttg
    50461 ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag tcgtgactac
    50521 agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccgcaa
    50581 ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg
    50641 tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt
    50701 acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg
    50761 cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt
    50821 ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa
    50881 aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc ctagtgaagc
    50941 cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta tctctaactc
    51001 aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt
    51061 aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg
    51121 tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcaccaccgc
    51181 cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc
    51241 tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt
    51301 aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg
    51361 tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa
    51421 tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt
    51481 ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac gtgtgcgccc
    51541 tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat
    51601 ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac tgcgtgcagg
    51661 cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc
    51721 tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg tctgctgatc
    51781 ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa cggcgaagag
    51841 ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt
    51901 gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg
    51961 gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag cgatttgctg
    52021 gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct catcgactgg
    52081 ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc
    52141 ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc
    52201 gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta
    52261 aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcatta
    52321 atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata
    52381 aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga
    52441 ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac
    52501 ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc
    52561 tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca cttgtgccag
    52621 actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg
    52681 ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa
    52741 tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg
    52801 aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc
    52861 agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac
    52921 cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt catcgtcacc
    52981 cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa
    53041 ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat gcaggacaac
    53101 ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa agattttgtc
    53161 gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg cgataagatc
    53221 acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaacc
    53281 accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac cccggcacag
    53341 aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat
    53401 aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt
    53461 ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt aggcattgtg
    53521 ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat
    53581 gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt ccgtcgcctc
    53641 agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa
    53701 cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc
    53761 atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct
    53821 gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt
    53881 atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt gctgatcact
    53941 gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg
    54001 catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt taagcgtgta
    54061 taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga
    54121 gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga
    54181 cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg
    54241 agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact catgaagaaa
    54301 tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa
    54361 aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta
    54421 caccatccgg caccagcggt gactacattg acgacgagct gccggtctac cagggcgaaa
    54481 cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg ttttccgaca
    54541 ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt tatctgactg
    54601 tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc gacgaggttg
    54661 aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca aaagatctgc
    54721 gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg ctggaagagg
    54781 ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc cgcactttaa
    54841 tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc
    54901 tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg
    54961 tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac agcattccgc
    55021 gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac gatggtgaca
    55081 gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc
    55141 gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg
    55201 agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag gctgtcgaaa
    55261 tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca
    55321 tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc ggcgaagctt
    55381 cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac
    55441 cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggcacgcc
    55501 gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacagc
    55561 tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc
    55621 gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact tgagcaatat
    55681 tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca
    55741 gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg
    55801 tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat
    55861 aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg
    55921 cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg
    55981 ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac
    56041 agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc
    56101 agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga agctatcctg
    56161 acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc gcatggcaaa
    56221 cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac gcctatagct
    56281 ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt gccggcagac
    56341 caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc ggacaaaacc
    56401 atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat
    56461 accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag
    56521 gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc
    56581 gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg
    56641 ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt cagcattgat
    56701 gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac
    56761 gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat tcgtcgttac
    56821 agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc
    56881 gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc
    56941 tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct gggtaaacgt
    57001 gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat
    57061 gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt
    57121 ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac agaagtacac
    57181 aattttatct accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac
    57241 aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat
    57301 attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat
    57361 cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc
    57421 tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg
    57481 aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt attgcgctgc
    57541 tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt ccacaggaag
    57601 aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa gattaatctt
    57661 catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat cacgtactga
    57721 aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc agaagaacct
    57781 taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc atactggaga
    57841 tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa gtcccctggg
    57901 caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct gtatcaagat
    57961 tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa agtactacgc
    58021 gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg gtaccgttga
    58081 gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg gcaagccttc
    58141 cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg cacaactgcg
    58201 ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga cgatgtcgct
    58261 gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc cggtcgtctg
    58321 cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt ggtgttgtct
    58381 gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg ctattgccct
    58441 gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc gcgaagctta
    58501 atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct gccgtaaaat
    58561 ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac gaagtggatt
    58621 aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg cgggtttgtg
    58681 gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt cggcgatatt
    58741 caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca gcgttaatcc
    58801 agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat tttggccgtc
    58861 tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt gtgcgccagg
    58921 gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg atcttctgcg
    58981 gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca gtcagaatgt
    59041 gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac cattaccgct
    59101 gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa aacggcgagg
    59161 acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa gcggctttta
    59221 ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc gccgcttctg
    59281 caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct tcatcaacca
    59341 cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc acttctgcgg
    59401 tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc ggcagcatcg
    59461 ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct tgtgccagcg
    59521 cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg cttaaattct
    59581 tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca tcggcctggg
    59641 ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca ttgcgcgttt
    59701 ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta tccggtgcaa
    59761 agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc gcttcatgaa
    59821 tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc ttcattgtga
    59881 tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg tacgcatcac
    59941 acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact gcgtgcggcc
    60001 tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca gccaagattt
    60061 ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt tttagtggct
    60121 tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt catctcttca
    60181 atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat ttgctgaccc
    60241 agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt atgtccttcc
    60301 tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct gacatagccc
    60361 ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc tacaagttcg
    60421 agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt ctcttctgtc
    60481 gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat cgcggttagc
    60541 gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag gtactcttgt
    60601 ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc gcgcggataa
    60661 cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg aatatccagc
    60721 aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc ggtcatggcg
    60781 acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac gttcagttca
    60841 atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc atcaaacgca
    60901 tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt tgaacctttg
    60961 ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg acgagaaacg
    61021 gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt cacctggtaa
    61081 tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga atcttccact
    61141 tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg gccttgctgg
    61201 gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg cgtcccgtcg
    61261 gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa tcctttttgt
    61321 tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa atcaaccggc
    61381 tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat atcgttaaag
    61441 atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc ctgctcggcg
    61501 gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag cggtgtacgc
    61561 aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg ttcaagcgca
    61621 tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc gtgacgttta
    61681 cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg cccgtccggg
    61741 taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt ttcatcggtt
    61801 tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag gtgaaccagt
    61861 tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga aaactcttta
    61921 tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg taagaatgag
    61981 gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat tttcagttgg
    62041 ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc ctgatttagc
    62101 tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg catctcctcc
    62161 agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc gacgaccacc
    62221 gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga acgaataaca
    62281 tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac cacaatggca
    62341 agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa cttcatcatc
    62401 aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt ccttcacgac
    62461 aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg agaaatgcgg
    62521 aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc gccgatccct
    62581 gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat cgattttcgc
    62641 accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg tggtagatca
    62701 cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc tccagttcaa
    62761 taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc acaatatgca
    62821 gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc gtttgcaaac
    62881 attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc ttcagcccgc
    62941 gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg cggttgatgc
    63001 gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact tcgtaaccct
    63061 ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc gggcgggtac
    63121 caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata gaacgcagca
    63181 cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag cgtttagcgc
    63241 ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc tgctgcgctt
    63301 catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg gtaccgtcac
    63361 ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc ccataacgac
    63421 gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc tgatagaccg
    63481 cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg acctggatca
    63541 atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa atttcatgca
    63601 caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc ttatggattt
    63661 taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa atgctggttt
    63721 tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca ggaattgact
    63781 gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat ttcagctatt
    63841 taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca tttgacctgt
    63901 atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc agcctgctca
    63961 tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc gaaaaggaca
    64021 acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca cgctctttct
    64081 gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga ggttcacgat
    64141 atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat cgcccacata
    64201 gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc ccgcatgcag
    64261 caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt gctgttacaa
    64321 aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt agcaaaaaac
    64381 tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc cgcacgccgc
    64441 atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg tgatgtcccc
    64501 actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat tgagcaaatt
    64561 tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct gtttatcgcc
    64621 cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg tagcctgtcg
    64681 aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg cttttatctg
    64741 gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg cttctccact
    64801 aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca taacggtgaa
    64861 atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa attccagacg
    64921 ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg ttctgactcc
    64981 agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat catccgcgcc
    65041 atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc ggaactgcgt
    65101 gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc gggcatcgtg
    65161 atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg tccggcacgc
    65221 tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat ctgggattac
    65281 cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat ggtgatcgac
    65341 actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa aagccgccac
    65401 ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga agatctgccc
    65461 gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta ccagaaacag
    65521 tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga aaacggtcag
    65581 gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag tcagccgcgc
    65641 attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc aatcgacccg
    65701 ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat gaacgtcttt
    65761 tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct gctctactcc
    65821 gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac gctggatatc
    65881 acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg cgacaaagcc
    65941 gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa tatcgctaaa
    66001 gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg tctggtcgat
    66061 caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg cgatccgcac
    66121 cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct tgcctatgaa
    66181 acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac cgtgatgctc
    66241 aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat gggcatctcc
    66301 accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca cgatgatgta
    66361 gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt tgaagacttc
    66421 cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc gattagccag
    66481 ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc ggacgtggtg
    66541 cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga atacgcgaag
    66601 ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac gccgggtgaa
    66661 aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg ctttgatacc
    66721 gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc ggaagcgatg
    66781 aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc gcgctatggc
    66841 actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt tactccggcg
    66901 tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa gccgggcgaa
    66961 ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg ctattcggtg
    67021 cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat cgaagactta
    67081 gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc cgttaagctg
    67141 gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta tgcggacttg
    67201 atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc agtgaaatat
    67261 gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt tgccaacggc
    67321 ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt cgatatcatc
    67381 aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat ggtggcgctc
    67441 ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt agcaactcag
    67501 gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac taattacttt
    67561 gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac gcgtctggtg
    67621 gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc caaacagcag
    67681 aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa gtcactctac
    67741 tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt gctgcaacag
    67801 gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat ccgcaacacc
    67861 gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg cgatcagggg
    67921 ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag tttcggtgtg
    67981 tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta tgtcggtaaa
    68041 ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt ccgcagccat
    68101 gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg tctgtatgcc
    68161 gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac cgtggtagaa
    68221 ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat tctgggtaaa
    68281 accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct cgatgaaagc
    68341 ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt cgacgatctg
    68401 gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca taccggttct
    68461 cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt cgcgctggtt
    68521 aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag cgcagcagag
    68581 ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta tcaatttatc
    68641 gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat tgagtttgtt
    68701 gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg ctgtctgtcg
    68761 tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc aaactggctg
    68821 aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca gaccaacaca
    68881 ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg ttcctgcacc
    68941 ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat caatgataaa
    69001 gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg taaaaaagtg
    69061 gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac gcgtaacggc
    69121 gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac cttcggtatt
    69181 ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt caccggcatg
    69241 ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga cgatctgctg
    69301 agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg cggcgggctg
    69361 gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc caacaccaaa
    69421 cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga aggcaaacgc
    69481 gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc tgtacgccag
    69541 ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc gggttcccgc
    69601 cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt ccagccactg
    69661 gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg taccgaaatg
    69721 ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc cgaacatatc
    69781 gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat ggaatggctg
    69841 gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga aggcaacgac
    69901 aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt ccgtggttcc
    69961 gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat tatgaactgg
    70021 ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc gctgaaattc
    70081 tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa tacgctcatc
    70141 tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac gcagcgccat
    70201 ataaagggac agataatgga atcgctctca gagagaacct caacaggcta ccagca
//
LOCUS       DAJTYM010000005        67017 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTYM010000005 DAJTYM010000000
VERSION     DAJTYM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            2..617
                     /locus_tag="OO575_000342"
                     /pseudo
     CDS             2..617
                     /locus_tag="OO575_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            720..1043
                     /locus_tag="OO575_000343"
     CDS             720..1043
                     /locus_tag="OO575_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR6663439.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1110..2567)
                     /gene="nuoN"
                     /locus_tag="OO575_000344"
     CDS             complement(1110..2567)
                     /gene="nuoN"
                     /locus_tag="OO575_000344"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6663440.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2574..4103)
                     /gene="nuoM"
                     /locus_tag="OO575_000345"
     CDS             complement(2574..4103)
                     /gene="nuoM"
                     /locus_tag="OO575_000345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6663441.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4267..6108)
                     /gene="nuoL"
                     /locus_tag="OO575_000346"
     CDS             complement(4267..6108)
                     /gene="nuoL"
                     /locus_tag="OO575_000346"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6663442.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6105..6407)
                     /gene="nuoK"
                     /locus_tag="OO575_000347"
     CDS             complement(6105..6407)
                     /gene="nuoK"
                     /locus_tag="OO575_000347"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6663443.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6404..6958)
                     /gene="nuoJ"
                     /locus_tag="OO575_000348"
     CDS             complement(6404..6958)
                     /gene="nuoJ"
                     /locus_tag="OO575_000348"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6663444.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6970..7512)
                     /gene="nuoI"
                     /locus_tag="OO575_000349"
     CDS             complement(6970..7512)
                     /gene="nuoI"
                     /locus_tag="OO575_000349"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6663445.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7527..8504)
                     /gene="nuoH"
                     /locus_tag="OO575_000350"
     CDS             complement(7527..8504)
                     /gene="nuoH"
                     /locus_tag="OO575_000350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6663446.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8501..11227)
                     /gene="nuoG"
                     /locus_tag="OO575_000351"
     CDS             complement(8501..11227)
                     /gene="nuoG"
                     /locus_tag="OO575_000351"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6663447.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11280..12617)
                     /gene="nuoF"
                     /locus_tag="OO575_000352"
     CDS             complement(11280..12617)
                     /gene="nuoF"
                     /locus_tag="OO575_000352"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6663448.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12614..13114)
                     /gene="nuoE"
                     /locus_tag="OO575_000353"
     CDS             complement(12614..13114)
                     /gene="nuoE"
                     /locus_tag="OO575_000353"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6663449.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13117..14919)
                     /gene="nuoC"
                     /locus_tag="OO575_000354"
     CDS             complement(13117..14919)
                     /gene="nuoC"
                     /locus_tag="OO575_000354"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6663450.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15013..15675)
                     /gene="nuoB"
                     /locus_tag="OO575_000355"
     CDS             complement(15013..15675)
                     /gene="nuoB"
                     /locus_tag="OO575_000355"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6663451.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15691..16134)
                     /gene="nuoA"
                     /locus_tag="OO575_000356"
     CDS             complement(15691..16134)
                     /gene="nuoA"
                     /locus_tag="OO575_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6663452.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16765..17703)
                     /gene="lrhA"
                     /locus_tag="OO575_000357"
     CDS             complement(16765..17703)
                     /gene="lrhA"
                     /locus_tag="OO575_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6663453.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18622..19839
                     /gene="alaA"
                     /locus_tag="OO575_000358"
     CDS             18622..19839
                     /gene="alaA"
                     /locus_tag="OO575_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6663454.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19923..20522
                     /gene="yfbR"
                     /locus_tag="OO575_000359"
     CDS             19923..20522
                     /gene="yfbR"
                     /locus_tag="OO575_000359"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6663455.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20581..22403)
                     /locus_tag="OO575_000360"
                     /pseudo
     CDS             complement(20581..22403)
                     /locus_tag="OO575_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
     gene            complement(22490..23140)
                     /gene="hxpA"
                     /locus_tag="OO575_000361"
     CDS             complement(22490..23140)
                     /gene="hxpA"
                     /locus_tag="OO575_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6663456.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23151..23645)
                     /locus_tag="OO575_000362"
     CDS             complement(23151..23645)
                     /locus_tag="OO575_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6663457.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23728..24183)
                     /locus_tag="OO575_000363"
     CDS             complement(23728..24183)
                     /locus_tag="OO575_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6663458.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24521..25723
                     /gene="ackA"
                     /locus_tag="OO575_000364"
     CDS             24521..25723
                     /gene="ackA"
                     /locus_tag="OO575_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6663459.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25798..27942
                     /gene="pta"
                     /locus_tag="OO575_000365"
     CDS             25798..27942
                     /gene="pta"
                     /locus_tag="OO575_000365"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6663460.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28132..29652
                     /gene="yfcC"
                     /locus_tag="OO575_000366"
     CDS             28132..29652
                     /gene="yfcC"
                     /locus_tag="OO575_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6663461.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29685..30227)
                     /gene="yfcD"
                     /locus_tag="OO575_000367"
     CDS             complement(29685..30227)
                     /gene="yfcD"
                     /locus_tag="OO575_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6663462.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30285..30836)
                     /gene="yfcE"
                     /locus_tag="OO575_000368"
     CDS             complement(30285..30836)
                     /gene="yfcE"
                     /locus_tag="OO575_000368"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6663463.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30892..31536)
                     /gene="yfcF"
                     /locus_tag="OO575_000369"
     CDS             complement(30892..31536)
                     /gene="yfcF"
                     /locus_tag="OO575_000369"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6663464.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31672..32319
                     /gene="yfcG"
                     /locus_tag="OO575_000370"
     CDS             31672..32319
                     /gene="yfcG"
                     /locus_tag="OO575_000370"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6663465.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32376..32738
                     /gene="folX"
                     /locus_tag="OO575_000371"
     CDS             32376..32738
                     /gene="folX"
                     /locus_tag="OO575_000371"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6663466.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32759..33652
                     /locus_tag="OO575_000372"
     CDS             32759..33652
                     /locus_tag="OO575_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6663467.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33700..34590)
                     /gene="rpnB"
                     /locus_tag="OO575_000373"
     CDS             complement(33700..34590)
                     /gene="rpnB"
                     /locus_tag="OO575_000373"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6663468.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34787..35560)
                     /gene="hisP"
                     /locus_tag="OO575_000374"
     CDS             complement(34787..35560)
                     /gene="hisP"
                     /locus_tag="OO575_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6663469.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35568..36284)
                     /locus_tag="OO575_000375"
     CDS             complement(35568..36284)
                     /locus_tag="OO575_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6663470.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36281..36967)
                     /gene="hisQ"
                     /locus_tag="OO575_000376"
     CDS             complement(36281..36967)
                     /gene="hisQ"
                     /locus_tag="OO575_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6663471.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37057..37839)
                     /gene="hisJ"
                     /locus_tag="OO575_000377"
     CDS             complement(37057..37839)
                     /gene="hisJ"
                     /locus_tag="OO575_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6663472.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38060..38842)
                     /gene="argT"
                     /locus_tag="OO575_000378"
     CDS             complement(38060..38842)
                     /gene="argT"
                     /locus_tag="OO575_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6663473.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39108..39677)
                     /gene="ubiX"
                     /locus_tag="OO575_000379"
     CDS             complement(39108..39677)
                     /gene="ubiX"
                     /locus_tag="OO575_000379"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6663474.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39772..41289)
                     /gene="purF"
                     /locus_tag="OO575_000380"
     CDS             complement(39772..41289)
                     /gene="purF"
                     /locus_tag="OO575_000380"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6663475.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41326..41814)
                     /gene="cvpA"
                     /locus_tag="OO575_000381"
     CDS             complement(41326..41814)
                     /gene="cvpA"
                     /locus_tag="OO575_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6663476.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42073..42735)
                     /gene="dedD"
                     /locus_tag="OO575_000382"
     CDS             complement(42073..42735)
                     /gene="dedD"
                     /locus_tag="OO575_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6663477.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42725..43993)
                     /gene="folC"
                     /locus_tag="OO575_000383"
     CDS             complement(42725..43993)
                     /gene="folC"
                     /locus_tag="OO575_000383"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6663478.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44063..44977)
                     /gene="accD"
                     /locus_tag="OO575_000384"
     CDS             complement(44063..44977)
                     /gene="accD"
                     /locus_tag="OO575_000384"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6663479.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45133..45792)
                     /locus_tag="OO575_000385"
     CDS             complement(45133..45792)
                     /locus_tag="OO575_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6663480.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45875..46687)
                     /gene="truA"
                     /locus_tag="OO575_000386"
     CDS             complement(45875..46687)
                     /gene="truA"
                     /locus_tag="OO575_000386"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6663481.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46687..47700)
                     /locus_tag="OO575_000387"
     CDS             complement(46687..47700)
                     /locus_tag="OO575_000387"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6663482.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47766..48902)
                     /gene="pdxB"
                     /locus_tag="OO575_000388"
     CDS             complement(47766..48902)
                     /gene="pdxB"
                     /locus_tag="OO575_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6663483.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49001..49996
                     /gene="flk"
                     /locus_tag="OO575_000389"
     CDS             49001..49996
                     /gene="flk"
                     /locus_tag="OO575_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6663484.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49993..51171)
                     /locus_tag="OO575_000390"
     CDS             complement(49993..51171)
                     /locus_tag="OO575_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6663485.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51426..52667)
                     /gene="fabB"
                     /locus_tag="OO575_000391"
     CDS             complement(51426..52667)
                     /gene="fabB"
                     /locus_tag="OO575_000391"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6663486.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            52826..54832
                     /gene="mnmC"
                     /locus_tag="OO575_000392"
     CDS             52826..54832
                     /gene="mnmC"
                     /locus_tag="OO575_000392"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6663487.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54953..55231)
                     /locus_tag="OO575_000393"
     CDS             complement(54953..55231)
                     /locus_tag="OO575_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6663488.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55265..55813)
                     /gene="epmC"
                     /locus_tag="OO575_000394"
     CDS             complement(55265..55813)
                     /gene="epmC"
                     /locus_tag="OO575_000394"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6663489.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55813..56622)
                     /locus_tag="OO575_000395"
     CDS             complement(55813..56622)
                     /locus_tag="OO575_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6663490.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56622..57446)
                     /gene="mepA"
                     /locus_tag="OO575_000396"
     CDS             complement(56622..57446)
                     /gene="mepA"
                     /locus_tag="OO575_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6663491.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57450..58535)
                     /gene="aroC"
                     /locus_tag="OO575_000397"
     CDS             complement(57450..58535)
                     /gene="aroC"
                     /locus_tag="OO575_000397"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6663492.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58570..59502)
                     /gene="prmB"
                     /locus_tag="OO575_000398"
     CDS             complement(58570..59502)
                     /gene="prmB"
                     /locus_tag="OO575_000398"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6663493.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59668..60219
                     /gene="smrB"
                     /locus_tag="OO575_000399"
     CDS             59668..60219
                     /gene="smrB"
                     /locus_tag="OO575_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6663494.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60323..61213)
                     /locus_tag="OO575_000400"
     CDS             complement(60323..61213)
                     /locus_tag="OO575_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6663495.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61200..61724)
                     /locus_tag="OO575_000401"
     CDS             complement(61200..61724)
                     /locus_tag="OO575_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6663496.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61721..62191)
                     /locus_tag="OO575_000402"
     CDS             complement(61721..62191)
                     /locus_tag="OO575_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6663497.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62188..62736)
                     /locus_tag="OO575_000403"
     CDS             complement(62188..62736)
                     /locus_tag="OO575_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6663498.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62741..63463)
                     /locus_tag="OO575_000404"
                     /pseudo
     CDS             complement(62741..63463)
                     /locus_tag="OO575_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63483..64205)
                     /locus_tag="OO575_000405"
     CDS             complement(63483..64205)
                     /locus_tag="OO575_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6663499.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64265..65419)
                     /gene="yfcU"
                     /locus_tag="OO575_000406"
                     /pseudo
     CDS             complement(64265..65419)
                     /gene="yfcU"
                     /locus_tag="OO575_000406"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(65368..66123)
                     /locus_tag="OO575_000407"
     CDS             complement(65368..66123)
                     /locus_tag="OO575_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6663500.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66205..66768)
                     /locus_tag="OO575_000408"
     CDS             complement(66205..66768)
                     /locus_tag="OO575_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6663501.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16692 a        18154 c        16690 g        15481 t
ORIGIN      
        1 tattatttct gttttcactc cctctgatta tttttaccaa ccctgggtca ctgcattatc
       61 gccaacgccc cgacacgtaa gtcctgagca attagaaaaa acagttcgtt atcttacaca
      121 aactgtgcat ccacgtaatg ccggcaatat tgataatctg aataggtccg cagagtatat
      181 aaaagagttc tttgtcagta gcggtgccag agttaccgcg cagggcgtcc ccgattggct
      241 attacgactc tgcgcctgga agccagaatt acccttaccc ggcaatgtcc tggctttatc
      301 ccgatcgggg agactttatt gctgtggtcg gcagaatgca ggatatcaac gccgttcgtc
      361 aggtaaaagc ggcattgttg tcatcccggg atttatctgt ttattctatg aatgccccag
      421 ggtttattcc cggtattgat ttttctgatc acctcaatta ttggcaacac gatattcccg
      481 ccgtaatgat tactgacacc gctttttatc gtaataaaca ataccacttg cccggtgata
      541 ccgcagacag attgaattat cagaaaatgg ctcaggtggt agatggtgtt ataaccttgt
      601 tatacaacag taaataataa aaatttctcg aggcgggtcc agaaacagcg acatgtacat
      661 tttattttcc ctcgtctgtt ttaagatgcg aaacataaaa ttcaatggag gaaaaataaa
      721 tgggaatgat tggctatttt gcagaaattg actcagaaaa gattaaccaa ctgcttgagt
      781 ctactgaaaa acctttgatg gataatatac atgacacgct ttcaggatta cgccgactgg
      841 aatcactgga tcgaagtgag ctgcgtaaac agttttccat taagcgactc aatgaaatgg
      901 agatttatcc gggagtgacc ttttcagaag agttagaggg gcaactcttt gcctcgatca
      961 tgcttgatat ggaaaagctg attagcgcgt atcgacgtat gctgcgacaa gggaatcatg
     1021 cgctgacggt tattgttggg tgatggtgtc agagcgccaa aaaaaaccgc cgaacatgtc
     1081 ggcggctttc tgacttacaa actaacagtt tacatcagcg gcattgctaa ccgcacaata
     1141 ctaatcagcg gttgtggcca tacacccagc accagtacca acagtgcaga aatcagcacc
     1201 acgataccgc ccgcgctgta ctgccagttt gacggtgcat cgcgacccgg ttgttccggt
     1261 gcgtgcaggt acaggctcac cgccacgcgc aggtagtagt agaggccgat tgccgaaccg
     1321 acaaccacgg cacccaccag ccaccacaag tgtgcctgga caccgaccgc cagcacgtag
     1381 aacttaccga taaagcccag cgtcatcggg ataccggcca gagacagcat catcaccgtc
     1441 atcactgccg cgaggatcgg acgatgccag aacagaccgc ggtaagagaa cagggaatca
     1501 gcatccgggc cacgatacgg gctggacatc aggctgacca cgccgaacgc gccgaggctg
     1561 ctgaacagat aaccggccag gtaaacccct accgcttcca tcgacatctc gccggtttgc
     1621 agcgcaatca gcgccaccag cagatagccg aggtgagaga tagatgagta accgagcaga
     1681 cgtttgatgt tggtctggct caacgccatc aggttaccga agatgatgga ggcaaaggcg
     1741 ataatcgcca gtactacgcg aatcgcttcg ctgtcaccca ccggtgcgta gaggaacaga
     1801 cgcatcacca caccgaagat agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc
     1861 gcaggcgcgc cctggtatac gtctggcgtc cacaggtgga acggcaccag agagagtttg
     1921 aagccgaggc caacaatcat catgccgaaa cctgccagca acagcggctc gttgagcata
     1981 ccgtcgccga ggtttttacc cagcgcgaca aacgacaggt cgccagactg cgcatacacc
     2041 agcgccatac cgaacagcag gaaagaagac gctgcggcag aaaggatggt gtatttgata
     2101 ctggcttcca gcgaacgttt ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa
     2161 gagatcagtt caataccgag gaacagagac gccagatggt ttgcattcgc cagcaggatc
     2221 ccgcccagcg cggcaattaa caccaacagg tagaactcat ccttgttgtc gttatagcct
     2281 tcaagccacg ggtaggcgaa agtacaggtg gcgaggctcg ccaacaatac cagcccggtg
     2341 taaagcatgg cgaaaccatc aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg
     2401 ccaacaaacc agagcgaaac cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg
     2461 aggaaatgat tgcgtcgcca cgcaatggag agcatcacaa ccaccaccgt caagccgacg
     2521 atcagcaacg gtagcagtgc gatcaggttt tgtggagtta ttgtcatggc gatttacggc
     2581 cttgtagtag taacggaatt aacaaaccac tgctggatat tgccaatcgc ggagtgcgag
     2641 gtatccagaa tcggctgcgg atagaagccc agcagtacca gcagcaccac cagcaacagg
     2701 atcataaaca gctcacgcag cgacatccct ggcagttcct ggctggcaat ctggcttttc
     2761 gctttaccga agtaagcgcg atgcaacatc gccagcgaat aaacagatgc aaagaccagc
     2821 ccaaaggtag agataacggt aatcaccggg acaacctgga agctgccgaa cagaatcata
     2881 aattcgccga cgaagttacc ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc
     2941 gacagtgctg gcagccattt catcttgctc cacagaccgc ccatcatgcg catgtcgcgg
     3001 gtatggatac gttcataaag ctgaccgcag agaataaaca gacccgccgc cgacaagccg
     3061 tgcgcaatca tctggattac cgcgccctgg taggccaact ggctgccggt gtagattgca
     3121 atcagcacga agcccatgtg ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc
     3181 tgagcgaagg tcatccacgc accgtagaag atgccgataa cacccagcca catagcgatt
     3241 ggcgcgaact ctgccgacgc gttcgggaac agcggcaggg agaaacgcaa caaaccgtaa
     3301 gcggcggttt tcagcaagat ccccgcgagg tccacggaac cggcggtcgg agcctgggag
     3361 tgcgcatccg gcagccagcc atgcagcgga accaccggca ttttgactgc gaaggcgatg
     3421 aagaagccca gcatcaacag atattccaca ccactggaca ttggcgtatt cagcagctct
     3481 tcatagttga aggtccagac gccggtcgca ttgtagtgaa caaaaaccag cgccaggatg
     3541 gcgatcaaca tcaccagacc actcgcctgg gtgtaaatga agaacttggt tgccgccgtg
     3601 atacgcgttt taccgtcaga ggctttatgc ccccacagtg cgatcaggaa gtacatcggc
     3661 accagcatca tttcccagaa gaagaagaac aggaacatgt cgatggcaag gaacacgccg
     3721 ataacgccgc ccaggatcca catcaggttg aggtggaaga agccctgata tttttcgatc
     3781 tctttccacg aacatagtac cgccagcaca ccgagcagac cggtcagcac gaccatcagc
     3841 agcgacagac cgtcaatggc gagatgaata gagataccaa aacgcgggat ccacggcatg
     3901 tcgaattcag actgccactg cggaattccg gcggattgcg tcagtgaata accgccctgc
     3961 aaccacagtt gcagcgacag cgccagcgtc aatcccatgg tgatcagcgc gatccagcgt
     4021 ggcaccttga cgccaaagcg ttcggtctgc cagcacagga agccgccaat aaagggaatt
     4081 aatattagcc agggtagtaa catggcgatc tttattcctt gtaaaagtcc cgtcaggacc
     4141 ggattttcaa cgaattctca cgacagaaaa ttctcttcgg actgggggta gtgtcggatg
     4201 cgccgcgaag gcagcttatc cgacctacgt tcttatatgc ccaggggcaa tcccacaatc
     4261 cttaactcaa cgcagtacca tcaacagtgc cagcaccacg accgcaccga tgctcatgga
     4321 tgccacatac cagcgcagat aaccgttctc gcttaacagc agacctttac ctgcaaagcg
     4381 ggaaagaacc gccgggatgt tcatcattga gttcagcgga tcgcgtttca gcaaccaggc
     4441 aatacccagg aacggcttga cgaacacttt gtcgtacagc cagtcaaagc cccaagcgtt
     4501 gtaccaccag gtactcagca ggcggcccgg cgcactgttg gcgatggagg tcaccagaga
     4561 acgtttaccc agccacagcc aggctgccag cagaatgccg accaccgcga ccacgccaga
     4621 ggtaatttcc agggtcaaca tgctgccgtg cgccagttcc gtcgtttgcg gaagcacgcc
     4681 ctgcagcggc ggtacaatca gtgcgccaac gaaggtggaa aggatcagca gcacaatcag
     4741 cggcaggctg tgagttaccc ctttcacggc gtgagcgtga atttgttctt ttccgtggaa
     4801 gacgatgaaa atcatacgga aggtgtagag cgaggtcata aacgcaccga ccagacctgc
     4861 caccatcaga ttgatatgac cattcgccat cgcacccgcg aggatctcat ccttactgaa
     4921 gaagcccgca gtgaccagcg gcagtgccga cagtgctgcg ccgcccacca ggaagcagag
     4981 ataaaccagc ggaatagatt tacgcagacc gcccatcttg aagatgttct gttcgtgatg
     5041 gcaggccaga atgacggaac cggatgccag gaacagcagc gctttaaaga acgcgtgagt
     5101 catcaagtgg aaaatcgccg catcccatgc ctgcacgcca agcgcgagga acatgtagcc
     5161 aatctggctc atggtagagt aagcgagaac acgtttgatg tcggtctgta ccagcgcggc
     5221 aaaaccggcc agcagcagcg taaccgcccc gacaataccc accagatgca gaacttccgg
     5281 cgtcatcagg aacaggccgt gggtacgggc gatcaggtag acacccgcgg ttaccatcgt
     5341 tgcggcgtgg atcagcgcgg agacaggcgt cgggcccgcc atcgcgtcag caagccatgt
     5401 ctgcaacggc aactgcgcag atttaccgac cgcaccgccc agcagcatca gcgtcgccca
     5461 catcagcatg ttattgccgt cagcaaagtg cgctggtgcc agttccacca tatcgcggaa
     5521 gttcagggtg cccagttcgt tgtaaagaat gaacagtgcg aaagcgagga aaacgtcacc
     5581 cacacgggtc acgacgaacg ctttcattgc tgctgcgcca ttcttcggat cggtgtaata
     5641 gaaaccgatc agcagatagg agcacaggcc cacgccttcc cagccgaggt acatcagcag
     5701 caggttatcg gcgagaacca gaactaccat gctggcgatg aacaggttgg tgtaggcgaa
     5761 gaagcgagag tagccctctt caccgcgcat gtaccaggag gcgtacatgt gaataaggaa
     5821 acccacacca gtgaccaccg agagcatggt cagcgacagg ccgtccagca ccaggttaaa
     5881 accgatgtta aagtcgccta ccgacatcca cgtccacagc ggctggctgt atgcctgctc
     5941 gccgttagcg aagaaatcaa cgccgataaa ggcggttacc agcgccgcca ggcccacaga
     6001 gccaacgccg acgatcgccg aaacgttttc agaccagcgc ccacgggaga acgccagcag
     6061 gacgaagcca atcaatggca aaataatggt taaggcaagc atgttcatcc gcgcatctca
     6121 cttactgaat cgatgttcag gttctggcga cgacggtgaa gttgcagcag cagcgcaagg
     6181 ccgatactcg cttctgccgc cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg
     6241 gtctgacccc agtagcttcc ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt
     6301 tccagaccaa tcagcataaa cagcagattg cgacggataa ccagaccggt taagccaaga
     6361 acgaataaga ttgccgcgag gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg
     6421 tttttctttt cgcgctgtcg tctttacgat tgctcagcac ttcacccgca cgctcttcac
     6481 gaccgacgtg gaaggccaca accagacccg cgagcagcag catagaagcc agttccaccg
     6541 ccagtacgta aggcccgaac agcgtaatac ccactgcttt agcactgatt ggcgtaccgt
     6601 cgataccctg atcgttaaca ccgaggatgg cgtaaacaat caccaccagc atgatggccg
     6661 acaaaattgc cgggccaatc cacacctgcg gtttcagcca ctggcgttcc tgttcgattt
     6721 ctgaaccgcc caggttgagc atcatcacca cgaacacgaa cagcaccata atggcacccg
     6781 cgtagacgat aatttccagc gcaccggcga agtaagcgcc tagtgagaag aacaccccgg
     6841 aaatcgccag cagcgaaata atcaggtaca gcagtgcgtg taccggattg gtatgggtga
     6901 tcactcgcaa ggtcgcaagt atggctatca ggccacagat ataaaaagcg aactccattg
     6961 cccctctcct tacggtaaca ggctcttgac gtcgataggc ttggcttcgt tctctgcttc
     7021 gcccttatct ttgccgtcga ttgccatacc tgccatccgg tagaagttat attccgggta
     7081 tttgcccgga ccggagatca gcagatcctc tttctcgtaa accagatcct ggcgcttgta
     7141 ttcccccatt tcgaaatccg gggttaactg aatcgccgtg gtcggacagg cttcttcgca
     7201 cagaccacag aaaatgcagc gtgagaagtt aatgcggaaa aattccggat accagcgacc
     7261 gtctttggtt tctgcttttt gcagcgagat acagccgacc gggcaggcta ccgcacagag
     7321 gttacaggct acgcaacgct cttcgccgtc cgggtcgcgg gtcagaacga tacgaccacg
     7381 ataacggggc ggcagataga ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa
     7441 cgcgtgcagg ccgatcatcc agatactacg aacctgggtg ccgaaaccta ctaacaattc
     7501 ttttaaggtc atggtcttat tgccccttat tgcgcctgcc agagaatgac agccgccgtt
     7561 accagcaagt tgatcagcgt cagcggcagg cagattttcc agccgaagga cattacccgg
     7621 tcataacgcg gacgcggtaa cgacgcacga atcaaaatga acatcatcat aaagaacgcg
     7681 gttttcagcg cgaaccagat gaatggcggt aacaacgggc cttgccagcc accgaagaac
     7741 agcgtcacca tcaatgcaga gatggtcaca atcccgatgt actcacccac gaagaacaga
     7801 ccgaacttca taccggaata ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc
     7861 ggctggtcaa acgggtgacg gtgacacacc gccacgcccg cgatggcaaa ggtaataaaa
     7921 ccaaagaatt gcgggatgac gttccacaca tgcgcctggc tgttgacgat gtcggtcatg
     7981 ttgaatgaac cggcctgcgc caccacgccc atcagggaaa gcccgaggaa cacttcgtag
     8041 ctcagggtct gcgcagaagc acgcatcgca cccagcagcg agtatttgtt gttacttgac
     8101 cagcccgcaa acagcaccgc gtaaaccgcc agacctgcca tcatcaggaa gaacaaaatc
     8161 ccgatgttca ggtcggcaac cacccaaccc ggactgactg gcacaattgc aaaggccagc
     8221 agcagcgagg taaaggcaat catcggtgcc agggtaaaga tgacgcgatc cgagaatttc
     8281 gggatccagt cttctttaaa gaacattttg atcatgtccg caaccagctg gagcgaaccg
     8341 ccccagccaa cacggttagg tccataacgg ttctggaaca gacccagcag gcgacgttcg
     8401 ccaaagctca tgaatgcccc acaggtgaca accaccagca ggatcaccac cgctttgagg
     8461 atggtcagca ggatctcaat cagttccggt gatatccaac tcattgttgt gcctccttga
     8521 gatcctcaag atgcgcgcca gccagcaccg gagcaatgcc ggacataccc atcggcaagc
     8581 ccacctgccc tgccgtcagt ccttcggcga tttcaaccgg cagcgtgacc gtgttgccat
     8641 cgtaactaaa ggagacgcgt gtacctgcgt tcacacccaa cttcgcggca tccgctgggt
     8701 tgagtttgat gtacggctgc ggcatacggc tctggaagac cggagcacgc tgtgacaatt
     8761 catcgctgcc aaacaggtgg tagtacggcg cgatacgcca tttcccgtcc tgcggctgga
     8821 agcgtgccgg tacgctggtg aagtaatcca gaccgttttc gctggtttca aacagacgca
     8881 cgcccggatc gccaaagcgc agtttgccgc ccacttcgtc ctggaatttg ttccacgcct
     8941 gcggggagtt ccagcccggc gcccaggcaa acggcacttg cgaacggtgc gcggtcggct
     9001 ggttgttacc ttccatcgag aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct
     9061 catgaacgct gatattggcg cgcatggcgg tacgaccgct gtaacggtgc ggttcacggg
     9121 ccagtttctg cccacgaata cggaatgtcg catccggcgc agcatctttg atacccgcca
     9181 gttctgggat tttcgccaca acagcgtcaa ttacatggtc gagctgcgtc cagtccactt
     9241 cacggctcag caaggtgctg tgcagcgagt gcagccagcg ccagctttcc agcatgacag
     9301 ttttgctgtc gtaataagcc ggatcgtaaa cctggaagaa acgttgggcg cgaccttcat
     9361 tattaattac cgtaccgtcg ctttcagcaa agctggcaga ggaaagcacc agatgggcgt
     9421 tttccatgat cgctgtgcgt tgatgatcaa ccaccatcac cagcggcgct ttagccagcg
     9481 cagcattcac gcgggtagca gaagcgtaac gatgcagatc gttttccaac accaccaccg
     9541 cgtcggcgcg tccggtttcc agttcggtta acgcttcttc aagcgaaccg ccacccataa
     9601 tgcccagccc catgctgttg acggaacggg caatcatggt gataccaacg tcagcgccgc
     9661 gacctttcag ggctttcgcg acgttagccg cagcctgaat cacctctatg ctaccggcgt
     9721 tcgtcccgga gataatcaac ggtttcttcg cacctgccag tgcctgcacg atgacgtcga
     9781 ttttgttttg cagctcaggt tcgataccgt caaccgctgg tgcggagtta tccagcgcat
     9841 gggcgatggc aaaacctaaa cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg
     9901 cgatatcatc cagacgagtg tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt
     9961 gaccgatgtt gaggattgcc gcaatctgcc agtcagccac tttctgtgct gcagccattt
    10021 cgcgcgcttt acctttcacc gcctgacgca ctgccagtgc gacgcgcgcg ccggtctggg
    10081 taacgtcttc gcccagcacc agtaccgcat cgtaagattc gatttcgcgc agagccggag
    10141 tataaatgcc gccttcacgc agcactttca gcgccagttg cagacgttcc tgctcaccgt
    10201 gagcgatacc ggtgtagaag ttttcttcgc ccaccagttc acgcagcgca aagttgcttt
    10261 ccacgctggc acgcggagaa ccaataccga tcactttctt cgactgacgc agaatatctg
    10321 ccgcgccctg cattgcctgt tcggcgttga gggtaatgaa atcatcgcca cgacgctgta
    10381 ctggctgacg cggacgatcc ttcaggttga cgtaaccgta accgaaacga ccacggtcgc
    10441 agaggaagta gtggtttacc gtaccgttgt aacggttttc gatacgacgc agttcgccgt
    10501 aacgttcacc ggggctgatg ttacagccga tggaacattg ctggcagatg ctcggcgcaa
    10561 actgcatatc ccatttacgg ttgtaacgct cggagtgcgt tttgtcggta aatacgccgg
    10621 tcgggcaaat ttcgaccagg ttaccggaaa attcgctttc cagcgtgccg tcttccgggc
    10681 gaccgaagta gacgttgtcg tgcgcgccat aaacgcccag atctgtaccg tcagcgtaat
    10741 ctttgtagta acgcacacag cggtagcagg cgatgcagcg gttcatttcg tgagagatga
    10801 atggccccaa atcctgatta cggtgggtac gtttggtgaa acggtagcga cggaagctgt
    10861 gtccggtcat cacggtcata tcctgaagat ggcagttacc gccttcttca cataccggac
    10921 agtcgtgcgg gtggttggtc attaaccact cgaccacact ttcacggaac tgtttcgctt
    10981 cttcgtcgtc aatggaaata aaggtgccat cggaagccgg tgtcatacag gacatcacca
    11041 ggcgaccacg cgtgtcttcc gcgttttggt attgcttcac cgcacactgg cggcaagcac
    11101 cgacgcttcc cagcgccgga tgccagcaaa agtaaggaat atcaaggccc agagacagac
    11161 aagcttccag caggttgtcc gctccgttga cctcgtattc tttgccgtct acatgaattg
    11221 tagccattag catgcttcca gttttctcgg tcagggaccg agcgttaatc gaaattcggt
    11281 taccagcgct ctttcagcag gttcggctga atcccattaa tcaaatgggt attgctgaac
    11341 ggctgtttga ttcccgcctc aaattcttcg cggaaatatt tgatggcgct ctgtaacggc
    11401 tccactgcac caggtgcgtg ggcacagaaa gttttacccg ggcctaagaa tcgacacagt
    11461 tgctcaagtg tttcgatatc gcccggctgg ccttcgccac gctccagcgc acgcagaatt
    11521 ttcacgctcc acggcagacc gtcgcggcac ggcgtacacc agccgcagga ctcacgggcg
    11581 aaaaactctt ccaggttacg caccagcgac accatgttga tctcatggtc aaccgccatc
    11641 gccagcgccg tacccagacg gctgcccgct ttaccgatac tttcgaattc catcggcaga
    11701 tcaaggtgcg cttcggtcag gaagtcagtc cccgcgccgc ctggctgcca ggctttaaat
    11761 ttcagaccat cacgcatacc accggcgtaa tcttcgagga tctcgcgtgc ggtggtgccg
    11821 aacggcagtt cccacagtcc cggatttttc acccgaccgg agaagcccat cagcttggtg
    11881 ccagcatctt tacttttcga gatgttctga taccactcca cgccgttagc gaggatcgcc
    11941 ggaacgttac acagggtttc gacgttgttg acacaggtcg gtttgcccca tacgccggag
    12001 gttgccggga aggggggttt cgagcgtggg ttagcacgac gcccttccag ggagttgatt
    12061 aacgctgttt cttccccgca gatgtagcgc cctgccccgg tatggacgaa cagttcgaag
    12121 tcgaaacctg ttcccataat gtttttgcca agcaagcccg cttcagtggc ttcggcaatg
    12181 gcgcggcgca gatttactgc cgcttcgata tattcgccac gcaggaagat gtagccacgg
    12241 taagctttca gcgcaaacgc ggagatgagc ataccttcca ccagcaggtg cggcagttgc
    12301 tccatcaaca ggcggtcttt ataggtgccc ggctccattt catcggcgtt acacagcagg
    12361 taacggatgt tcatggattc gtctttcggc atcaggctcc acttcaggcc agtcgaaaag
    12421 cccgcgccgc cgcgcccttt cagaccagcg tcttttacct gattaacgat ttcgtccgga
    12481 gacagcccgg tcagcgcctt acgcgcccct tcgtaaccgt ttttgctgcg gtattcgtcc
    12541 agccacactg gctgtttgtc atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta
    12601 cggataatgt ttttcattta taccgctcca gcagttcagg gatcgcttcc ggggtcagat
    12661 gcgcgtgagt gtcctcatcg atcatcatgt ttggcccttt atcacagttc cccaggcagc
    12721 aagttggcag cagcgtaaag cggccatcaa atgtcgtttg ccctggtttg atgttcagct
    12781 ttttctcgag cgccgcctga ataccctgat acccgttgat atgacagacc acgctgtcgc
    12841 aataacggat cacatggcga ccaaccggct ggcggaagat ctgactgtag aacgttgcca
    12901 caccttcgac gtcgcttgcc ggaataccca gcacatcggc gatcgcgtgg atcgcaccat
    12961 ccggcaccca gccacgctgc ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg
    13021 ggtcttcgta gtggtgcatc tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa
    13081 aagcctcggt ttgtggttgt tgattctcgt gcataattag cggtccacat ctgacataac
    13141 aaaatcgata ctgcccagat aaacaatcag gtcagacacc aggctgccgc ggatcgccgc
    13201 cggaatttgc tgcaaatgcg cataactcgg cgtgcggata cgagtgcgat aactcatggt
    13261 gctaccgtcg ctggtcaggt agtaactgtt gatccccttg gtcgcctcaa tcatctggaa
    13321 agattcattg gcaggcatca ccggccccca cgacacttgc aggaagtggg tgatcagggt
    13381 ttcgatatgt tgcagcgtgc gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt
    13441 gaacgggcct tccggcatgt tgttgaggca ctgctcaaga atgcgcagac tctggcgcag
    13501 ctcttccact ttcagcatta cgcgggtgta gcagtcagaa acaccaccac caaccgggat
    13561 ttcaaagtcg aagttttcat agccagaata aggacgcgcc ttacgcacgt cgaagtcgat
    13621 cccggtagca cgcaggcccg cgccagtggt gccccactcc agcgcctctt tcgcgccata
    13681 ggcggcaacg ccctgggaac gacctttcag aatggtattt tgcagcgccg ctttctcgta
    13741 agacgccaga cgtttcggca tccagtcgag gaactcacgc agcagacgat cccagccgcg
    13801 cggcaggtcg tgcgctacgc cgccaatacg gaaccacgcc gggtgcatac ggaaacccgt
    13861 gatcgcttcc accagatcgt aaattttctg acgatcggta aaggcgaaga acacgggcgt
    13921 cattgcgccg acgtcctgaa taaaggtcga gatgtacagc aggtggctgt tgatacggaa
    13981 cagttcggag agcataacgc gaatgacgtt aacgcgatcc ggcacggtga tcccggccag
    14041 tttctctacc gccagcacgt aaggcatttc gttaacgcag ccgccgaggt attcaatacg
    14101 gtcggtatac ggaatgtagc tgtgccagga ctggcgctcg cccattttct ccgcaccacg
    14161 gtggtggtag ccgatgtctg gtacgcagtc gacaatctct tcgccgtcga gttgtaaaac
    14221 gatacggaaa gccccgtgcg ccgacgggtg gttcggaccg aggttgagga acatgaagtc
    14281 ctcattttcg gtgccgcgtt tcatccccca ctcttccggt ttgaaggtca gggcttccat
    14341 ctccagatcc tgtttggctt tggtcagctc aaacggcgag aattcggtag cgcgtgccgg
    14401 gtaatcttta cgcagcgggt gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt
    14461 cgggtgaccg tcgaaagtaa tgccaaacag atcccaggtt tcacgctcat accagttagc
    14521 gttcgggaac agtttggtga aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt
    14581 cagcatgatg tcgcggttac gatcgataga aatcagatgg tagaaaacgg aaaaatccgc
    14641 ggcaggtaac ccttcgcggt gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag
    14701 catgacgtaa ggtttcggca gtttctttaa gaaatcgcca acttccagta attgttcacg
    14761 cttgatccac acaacgggaa ccccggtgcg agtcgcctga acagtaaagg catccggccc
    14821 aaaacggttg cgcagttcgc caattaccgg atcatcaaga tgatcgcggg tctgccaggc
    14881 gggttcttgc gcggttaagt cggtcatatt gttcaccatt gcaaatggtc cgtggtgact
    14941 gtcggcgcag taagcttcgc gaaatagatt taggaataag cgaagaaaaa tccctttgcc
    15001 gacaggcgca aattaaatct cgtcaggtgt acgcaggttg gttacggcaa tgcgttcacc
    15061 gcgcttgcgt tcgcgctctg attgcatatt ggcgcgataa acgccctgat cgccaaccac
    15121 ccaggagagc ggacgacgtt ctttgccgat agattcctgc aacagcatca gtgcctgcat
    15181 gtacgcttca ggacgcggcg ggcagcccgg gatatacaca tcaaccggga tgaatttatc
    15241 gacgccctgc acaacggaat aaatatcgta cataccacca gagttggcac aggcacccat
    15301 tgagataacc cattttggtt ccagcatctg gtcatacaga cgctgaataa ccggtgccat
    15361 tttggtaaag caggttcctg caaccaccat caggtcagcc tgacgcggcg aagcacgcaa
    15421 tacttctgcg ccaaaacgcg ccacgtcatg caccgcggta aacgaagtca ccatctcaac
    15481 gtaacagcag gaaagaccga agttatacgg ccaaattgag tttttacgac cccagttaac
    15541 catgtcattg agcttgccca taaacacgtt tttgttaact tcttgctcca gagggtcggt
    15601 tacgatctcc tgcttttgca gggggtaacg gtcgttctca ccgttgggat ctatgcgggt
    15661 gagcgtataa tccatcttaa tgcctcgcgg ttagcgttga cgattagcga tactgttcgt
    15721 ttccgggttc atacgctcgc ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg
    15781 caccagataa accagacctg ccagtaacac aaaaataaaa attgcagctt ccacaaagcc
    15841 tacccagccg ctttcgcgga tagaggttga ccatgcgaac agatacagcg cttcaacgtc
    15901 gaagataacg aagaacatgg ccaccagata aaacttggcg gacaggcgta agcgggcgga
    15961 gccgaccgag tcgataccgg attcaaacgg cacgtttttc gacctcgcgc gtgcgcgacc
    16021 gcctaaaaac caaccgccta ccagcatcag gcaacacagg ccaatggcaa cgataagaaa
    16081 gatagcgaat gcccagtgat gagcgatgac ttcagtggat gttgacatac tcattgctta
    16141 ctcatcaaaa gtagcgccag attcactgct cttcacggca gatggacgcc acatcgattc
    16201 atggggagga ataaaaaaaa ccttacaata actgtagaaa ttcttttata cagctaattg
    16261 atgtggtctt ttactccttt ctataacctt ttgtcaactt taacaaaagc ttcttcacat
    16321 tagtttacat aatatcaaca ccattagcat ttaatgccct ttcaccccag atccttgacg
    16381 actccaggat aattagatgt tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg
    16441 ggtctatttt gacaggaatt tgtgtcgatt cctcccccca aaagagagta ttttcttgat
    16501 ctgtgacacg cttttgttat tccataacaa aaacgcagca acaaatttac gtatttttta
    16561 acatcattgt agcaggtgat ttttttcagg cgattatttg tgcattcggg acgtgaatct
    16621 ctggtgattg aaaaataaac agttttgtac gttctgcact atgcgaggaa ggatttttac
    16681 taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgttttttac actattgtct
    16741 caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca atgaatcatc
    16801 cccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct ccatcgcctg
    16861 atagatcacc tgggccagct cattattact ggacgggtca tagcacagca aatattcggt
    16921 atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtccg ggctcatcat
    16981 ctcaaccggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa cggccggaag
    17041 cgtcgaggcg acataagcca gtcgccatgg aatatctact ttattaagcg tcgccagcac
    17101 catatcgcga aacggacttg gatcatccag caatacaaga gggatcggct cgcctttttg
    17161 caatacgtat tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt
    17221 aaacgctgaa ggacgatggg tggttaccat caaatccact tcctgggatt ccagcatttc
    17281 tgccatatag gcattacgtt taacgcggac atccagcgct aatttcggat aaaccgaact
    17341 cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag caccgatagt
    17401 taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat taaaacgcag
    17461 gattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt tgttgcgacc
    17521 gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt gctgacttac
    17581 ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca gatcggcaac
    17641 agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat ttgcacttat
    17701 catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc acacacaaac
    17761 aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg gacgaaagta
    17821 aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata atgcctgaag
    17881 tagatcacag aatatatctt cagggatcgc atatctatta agttactcac tcttttctat
    17941 ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac tctcgtgcag
    18001 catcgctggt catacgcgaa cacgtacaaa cagcaatggt gaagcggcat caagagcagg
    18061 gatccgtcat ttatgcgagc attttacccc aaaaaccttt tatttataag ggtcattgcg
    18121 aattatctga tgcaaagtta tgttatgtta ggcaaagtaa tcttctatta ttaataagca
    18181 catcaaaacc tttttgaata ttaaataata attaattagc atcatcctca ttcattaatt
    18241 ccgcttaaca atagtttcac accttacccc cattgcgaca cctcggaaat catcacgctg
    18301 ttaaactctc cgtaatgcgg aaatatcatc atcgcgtttc attagtgaat tcttaaatga
    18361 ggcattttca cacaattatc ttacagataa aaaaaccaga cttgcaatta agaattagaa
    18421 caattcacta tataacattg catgtaaagc atataagcct cattatttgt cattattaag
    18481 ttattaacag cacaatcgag ccttcccctc tggcaaaatc ttattctgca gaccttcaaa
    18541 acaccgtcct gggggagtac attgttctaa gctgacttcc acggcaggga gtggcgataa
    18601 cagcaaaaaa ggtcaagatt catgtccccc attgaaaaat ccagcaaatt agagaatgtc
    18661 tgttatgaca tccgtggtcc ggtgctgaaa gaagcaaaac gcctggaaga agaaggtaac
    18721 aaggtactga aactgaacat cggcaaccca gccccattcg gttttgacgc gccagatgaa
    18781 atcctcgttg acgtgatacg caacctgcct accgctcaag ggtattgcga ttccaaaggg
    18841 ctttactccg cgcgtaaagc catcatgcag cactaccagg ctcgtggcat gcgtgatgtt
    18901 accgtggaag atatttacat cggcaatggt gtatcggagc ttatcgttca ggcaatgcag
    18961 gcattgctga acagcgggga cgaaatgttg gttcctgcac cagattaccc actctggacc
    19021 gcggcggttt cgctttccag cggtaaagcg gtgcattatc tttgcgatga atcctctgac
    19081 tggttcccgg acctcgatga tattcgcgct aaaattacgc ctcgtacgcg tgggattgtt
    19141 attatcaacc caaataaccc aaccggcgcg gtatattcca aagagctttt aatggagatt
    19201 gtggagattg cacgtcagca taatctcatt atcttcgccg atgaaattta tgacaaaatt
    19261 ctctacgacg atgctgagca tcactcaatt gcgccgctgg cacctgacct gctgaccatt
    19321 acctttaacg gactgtcgaa aacgtaccgc gttgcaggct tccgtcaggg gtggatggtg
    19381 ttgaacgggc cgaaaaaaca cgccaaaggc tacatcgaag gtctggaaat gctggcttca
    19441 atgcgcctgt gtgctaacgt tcctgcgcaa cacgccattc agaccgcgct gggtggttat
    19501 cagagcatca gtgaatttat tacccctggc ggtcgtcttt atgagcagcg taaccgcgcg
    19561 tgggaactga tcaacgatat tccgggcgtt tcctgcgtga aacctcgtgg tgcgctgtat
    19621 atgttcccga aaatcgacgc caaacgcttt aacattcatg acgatcagaa aatggtgctg
    19681 gatttcctgt tgcaggaaaa agttctgttg gtgcaaggga cggcattcaa ctggccgtgg
    19741 ccggatcact tccgcattgt cacgctaccg cgtgtcgatg atatcgagct gtctttgagc
    19801 aagttcgcgc gtttcctttc tggttatcat cagctgtaat cttaatttca ctgccggaga
    19861 ttgcatccgg cagtgttatc ccgccacaat gacctgatga tgtcatcata cgtaaggtca
    19921 ctatgaaaca gagccatttt tttgcccatc tctcccgcct gaaactcatt aaccgctggc
    19981 cgctaatgcg caacgtgcgg acggaaaatg tgtccgaaca cagtttgcag gtagcgatgg
    20041 tcgcccatgc gctggcagct atcaaaaatc ggaaatttgg cggtaatgtc aacgccgaac
    20101 gtatcgcttt actggcgatg taccacgatg cctcagaagt gctcaccggc gatctcccta
    20161 ctccggtgaa atacttcaat gcgcaaatcg ctcaggagta caaggctatt gaaaaaatcg
    20221 ctcagcagaa actggtcgat atggttccgg aagaactgcg ggatatcttt gcgccgttaa
    20281 ttgacgagca tgcatatagc gatgaagaaa aatcgctggt gaaacaggca gatgcactgt
    20341 gtgcatatct gaaatgtctg gaagaactcg cggccggaaa taatgaattc ttgctggcaa
    20401 aaacgcgact ggaagcgacg cttgaagcgc gtcgcagcca ggagatggac tacttcatgg
    20461 aagtatttgt tcccagcttc catctttcgc tcgatgagat tagccaggat tcaccgctgt
    20521 aagcagccgg agtctgcgtc gcatcagtca ataagcgccg gatgcgacat caggctcttg
    20581 tcaaaacgga aacagcatcg ggatcatcac cacacaaacc gccatcacga taatggtgaa
    20641 cggtaccccc aacttcacaa agtcactgaa gctgtaattt cccggaccta aaaccagggt
    20701 gttaacaggt gaagacaccg gtgtcataaa ggcggcggat gctgccatcg ccacgaccat
    20761 cgcaaatgga taaggcgaca ctcccatcgt tttggcagca gccagcgcaa tcggagccat
    20821 caacaccgcc gtcgcggtat tagagataaa cagcccaata accgccgaca agacaaacaa
    20881 acaccccagc atcatatgtg gcccataacc gccgccaata tccatcagcc ctttcaccgc
    20941 cagcgcgaca cctcccgttt tctgtaatgc cacagcaaac ggcatcatcc caacgatcaa
    21001 ggccagtgaa tggatttata ggcgctttca gcatctatac agcggaattt ccccatcagc
    21061 aggcaggcga tgatagcggc aacaggatta ggaatttcat ttgtcagcat taacgccacc
    21121 attagcacca gacagaaaat ggcatggggt gcctggctgt gcgcgggcga tgcttcactc
    21181 acctcttccg gtaagttcag cgctacgaag tcgcggccct gtttggccag cataccgatc
    21241 agtttccagt tacccacaac caggatgata tcgcccagca gcagaggctc atccgccagc
    21301 gaaccttcca gcgccacgcc attgcgcttg agccccacca cattcagtcc gtagcgggta
    21361 cgaaaaccaa tttcgcgcac cgatttacca atcagttctg actcaggaat taatgaaatc
    21421 tctgccatgc ccacatcaag ggcctggtca gaaaaatact cgccgcgcag taccatcggc
    21481 tccagcaatt gctcactaca aaattgccgg agatcgacat cagccgcaga catatcaata
    21541 agcaaaacgt cacgcgcgcg aaattcagaa accccattaa cgttcacgat aacgcgacga
    21601 aaacgccgcc agcgttcaac accgatgacg ttagcgccat aacgctcacg taatttgaga
    21661 tcatccagcc gttgacctat cattggcgat ccggggcgaa tagccagacg tcgcgcacgc
    21721 ccggtcagtc gatattcacg gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc
    21781 tgcggggtct gggtatcccc tttcagcatg aaacgcatca ctaacatata caaaataccc
    21841 agcaccagca cgaccaggcc aataggtgtt acgctaaaga aactgaagcc gtgatagcct
    21901 tcacgcagca attcactgtt gactaccagg ttcggcggcg tcgccaccag cgtcatcatg
    21961 ccgctaatca gcccggcaaa acttaacggc atcatcagac gtgacggcga agtttgcata
    22021 cgcatggcaa cgcttaacac cacgggaata aagatagcga caacgctggt tgaactcata
    22081 aacgcgccaa gccccgcgac ggtcagcatc aacaaaacca acattttgat ttcactgttg
    22141 cccgccactt tgaccagcca tgttcccatt acggtggcaa caccggtacg gaccaaacca
    22201 tcgccaataa taaacaaggc ggcaatcagg acaacgttag gatcagaaaa gccggaaaat
    22261 acttcgggga ccgtcagcgt tccgcttaat gcaaacgcga caataacaaa caaagcgacc
    22321 gcatccatac gcactctgcc cgtcgcaaac aagacgatgg caacggccag taatgaaaga
    22381 acccaaatca attcaccgtt cacaacttat ccttgttaat tgagggggat gacttgattc
    22441 tgccataaaa aagccccgac gagacggggc taaatcatga tcaggtgttt cactgaataa
    22501 taacatcgcc atttggctgt ttggtcacag taatttgctc cagactgtgg aggaccaaat
    22561 cgacctcatt caggcgcggg gtatctgccg gagcgttaac cgcaatgaca tggcaacccg
    22621 ccgccaggcc agaaagcacg ccagcgggag catcttccac caccacacac tcctgcggcg
    22681 caagccccag cagctgcgca cctaacagat acgcatcagg ttctggtttt ccgcgcttca
    22741 ctcgctcagc ggttacaaac acctctggtg cgggaagccc cgctatttta tggcgcgctc
    22801 gcgctaccgg catggagcca gaagtcacaa tggcccacgg aatacctgct ttattcaaat
    22861 gattgagtaa ggcgattgcc ccaggaagcg cggtaatacc ttcggtttcc gtggcctcga
    22921 tttgctccag acgcgtaaac tcggcggcaa tatcagcctc ggatttgccc gccataaaat
    22981 ggcgcaaaga ggtgatcgcc tgtttaccgt gaatgaaagc cagcacctct tccggcgcta
    23041 acccatgacg tctggcccag ttgctccacg cccgttctac cgcaggcagg gaatccacca
    23101 gcgttccatc aagatcaaac agaaaacctt tgcaccgcac gcgggcctcc tcaggcgttg
    23161 ataatttgat taatttcgtt ggcgctcaaa tggtactgac gcgggcaggc atgccacaca
    23221 ttaagcatgc gctgatattt ttcccacatt ggcgtctggg cgttaaaacc atgagtccca
    23281 gcgtcaaaat gggtatagcg cccttccaca ttaaccataa agcggacata accgaggtaa
    23341 cgtgcttcag tggcggcgtc aaagccgagg aaggtgacac gacgttcatc gatggattgc
    23401 tggtcctgca aattagacca agaaacatac aacgcatgat acatatccat aatgtcgatg
    23461 atagtgcggc aggtttcttc tttcagctcg ccaaactcgc gatccaattc acgcatctgt
    23521 aatccgtaac cacgctcaat aattgtttgc aggcgacggt aacgttcagc atttgccgga
    23581 tcgagcatag tcatcatctt gtactgatta gacaaaataa gacgttgcgc gttggtcatt
    23641 tccattgttg actcctgtat cactctacta cggtgaaaaa aaagaaggct gagtatgcct
    23701 tcttttatat gcgtaatcag gggtcaatta caaatcatca aggaaagttt tatccagttg
    23761 tttgaaggcg cgcttaagcg tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg
    23821 tgccaacacc tgtccagact cctgcaattt accgcgaact tcataaaacc agttaaggat
    23881 tgcagggggt aatggcgtta cagaacgctt gcccagccac cacaatccct gcatgggtaa
    23941 acttaaggcg aacagtgcag tggcaactgc cggcccaagc agaccgccca gggcaatctg
    24001 ccagcagaga gtaaatacgg cgatcggcgg cataaaacgg atcgcataac gcgtcatctt
    24061 gataacgcga ttttcgacaa agaccggagc aaggcgtttt tccagtggcc acgtctttga
    24121 gtaatgctgt ccgcggcgaa acaagctaaa aaaattaaca gaacgattat ccggcgttga
    24181 catgcttcac ctcaacttca catataaaga ttcaaaaatt tgtgcaaatt cacaactcag
    24241 cgggacaacg ttcaaaacat tttgtcttcc atacccacta tcaggtatcc tttagcagcc
    24301 tgaaggccta agtagtacat attcattgag tcgtcaaatt catatacatt atgccattgg
    24361 ctgaaaatta cgcaaaatgg catagactca agatatttct tccatcatgc aaaaaaaatt
    24421 tgcagtgcat gatgttaatc ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc
    24481 ctgacgtttt tttagccacg tatcaattat aggtacttcc atgtcgagta agttagtact
    24541 ggttctgaac tgcggtagtt cttcactgaa atttgccatc atcgatgcag taaatggtga
    24601 agagtacctt tctggtttag ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa
    24661 aatggacggc aataaacagg aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct
    24721 caactttatc gttaatacta ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc
    24781 tatcggtcac cgtatcgtac acggcggcga aaagtatacc agctccgtag tgatcgatga
    24841 gtctgttatt cagggtatca aagatgcagc ttcttttgca ccgctgcaca acccggctca
    24901 cctgatcggt atcgaagaag ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc
    24961 tgtatttgac accgcgttcc accagactat gccggaagag tcttacctct acgccctgcc
    25021 gtacaacctg tacaaagagc acggcatccg tcgttacggc gcgcacggca ccagccactt
    25081 ctatgtaacc caggaagcgg caaaaatgct gaacaaaccg gtagaagaac tgaacatcat
    25141 cacctgccac ctgggcaacg gtggttccgt ttctgctatc cgcaacggta aatgcgttga
    25201 cacctctatg ggtctgaccc cgctggaagg tctggtcatg ggtacccgtt ctggtgatat
    25261 cgatccggcg atcatcttcc acctgcacga caccctgggc atgagcgttg acgcaatcaa
    25321 caaactgctg accaaagagt ctggcctgct gggtctgacc gaagtgacca gcgactgccg
    25381 ctatgttgaa gacaactacg cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg
    25441 ccaccgcctg gcgaaataca tcggtgccta cactgcgctg atggatggtc gtctggacgc
    25501 tgttgcattc actggtggta tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg
    25561 caaactgggc gtgctgggct ttgaagttga tcatgaacgc aacctggctg cacgtttcgg
    25621 caaatctggt ttcatcaaca aagaaggtac ccgtcctgcg gtggttatcc caaccaacga
    25681 agaactggtt atcgcgcaag acgcgagccg cctgactgcc tgatttcaca ccgccagctc
    25741 agctggcggt gctgttttgt aacccgccaa atcggcggta acgaaagagg ataaaccgtg
    25801 tcccgtatta ttatgctgat ccctaccgga accagcgtcg gtctgaccag cgtcagcctt
    25861 ggcgtgatcc gtgcaatgga acgcaaaggc gttcgtctga gcgttttcaa acctatcgct
    25921 cagccgcgta ccggtggcga tgcgcccgat cagactacga ctatcgtgcg tgcgaactct
    25981 tccaccacga cggccgctga accgctgaaa atgagctacg ttgaaggtct gctttccagc
    26041 aatcagaaag atgtgctgat ggaagagatc gtcgcaaact accacgctaa caccaaagac
    26101 gctgaagtcg ttctggttga aggtctggtc ccgacacgta agcaccagtt tgcccagtct
    26161 ctgaactacg aaatcgctaa aacgctgaat gcggaaatcg tcttcgttat gtctcagggc
    26221 actgacaccc cggaacagct gaaagagcgt atcgaactga cccgcaacag cttcggtggt
    26281 gccaaaaaca ccaacatcac cggcgttatc gttaacaaac tgaacgcacc ggttgatgaa
    26341 cagggtcgta ctcgcccgga tctgtccgag attttcgacg actcttccaa agctaaagta
    26401 aacaatgttg atccggcgaa gctgcaagaa tctagcccgc tgccggttct cggcgctgtg
    26461 ccgtggagct ttgacctgat cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg
    26521 accatcatca acgaaggcga catcaatact cgccgcgtta aatccgtcac tttctgcgca
    26581 cgcagcattc cgcacatgct ggagcacttc cgtgccggtt ctctgctggt gacttccgca
    26641 gaccgtcctg acgtgctggt ggccgcttgc ctggcagcca tgaacggcgt agaaatcggt
    26701 gccctgctgc tgactggcgg ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt
    26761 gctttcgcta ccggcctgcc ggtatttatg gtgaacacca acacctggca gacctctctg
    26821 agcctgcaga gcttcaacct ggaagttccg gttgacgatc acgaacgtat cgagaaagtt
    26881 caggaatacg ttgctaacta catcaacgct gactggatcg aatctctgac tgccacttct
    26941 gagcgcagcc gtcgtctgtc tccgccagcg ttccgttatc agctgactga actggcgcgc
    27001 aaagcgggca aacgtatcgt tctgccggaa ggtgacgaac cgcgtaccgt taaagcagcc
    27061 gctatctgtg ctgaacgtgg tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc
    27121 aaccgtgttg cagcgtctca gggtgtagaa ctaggtgcag gcattgaaat cgttgatcca
    27181 gaagtggttc gcgaaagcta tgttggtcgt ctggtcgagc tgcgtaagaa caaaggcatg
    27241 accgaaaccg ttgcccgcga acagctggaa gacaacgtgg tgctcggtac gctgatgctg
    27301 gaacaggatg aagttgatgg tctggtttcc ggtgctgttc acactaccgc aaacaccatc
    27361 cgtccgccgc tgcagctgat caaaactgca ccgggcagct ccctggtatc ttccgtgttc
    27421 ttcatgctgc tgccggaaca ggtttacgtt tacggtgact gtgcgatcaa cccggatccg
    27481 accgctgaac agctggcaga aatcgcgatt cagtccgctg attccgctgc ggccttcggt
    27541 atcgaaccgc gcgttgctat gctctcctac tccaccggta cttctggtgc aggtagcgac
    27601 gtagaaaaag ttcgcgaagc aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc
    27661 gacggtccgc tgcagtacga cgctgcggta atggctgacg ttgcgaaatc caaagcgccg
    27721 aactctccgg ttgcaggtcg cgctaccgtg ttcatcttcc cggatctgaa caccggtaac
    27781 accacctaca aagcggtaca gcgttctgcc gacctgatct ccatcgggcc gatgctgcag
    27841 ggtatgcgca aaccggttaa cgacctgtcc cgtggcgcac tggttgacga tatcgtctac
    27901 accatcgcgc tgactgcgat tcagtctgca cagcagcagt aatctcgtca tcatccacag
    27961 ctttgcgctg cggatatctg aaccggaaat aatcactatt tccggttttt tattctctta
    28021 atttgcatta atcctttctg attatcttgc ttaactgcgc tgcatcaatg aattgcgcca
    28081 tctcactttg catacttacc actttgtttt gtgcaaggga atatttgcgc tatgtccgca
    28141 atcactgaat ccaaaccaac aagaagatgg gcaatgcccg atacgttggt gattatcttt
    28201 tttgttgcta ttttaaccag tcttgccacc tgggtagttc cggtggggat gtttgacagt
    28261 caggaagtgc agtatcaggt tgatggtcaa acaaaaacac gcaaagtcgt agatccacac
    28321 tcatttcgca ttctgactaa cgaagcaggc gaacctgagt atcaccgcgt acagctgttc
    28381 acgacgggcg atgaacgccc gggcctgatg aacttcccgt ttgaagggtt aacctcagga
    28441 tcgaaatacg ggacagccgt tggcatcatc atgtttatgc tggtgattgg cggcgcgttt
    28501 ggcattgtga tgcgtacagg aaccattgat aacggtatcc tggcgcttat tcgccatacc
    28561 cgcgggaatg aaattctctt tattcctgcg ctgtttattc tgttttcact tggcggcgcg
    28621 gtatttggta tgggagaaga ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc
    28681 cggctgggct atgacagtat taccaccgtc ctggtgactt atattgccac gcaaatcggt
    28741 tttgccagtt cgtggatgaa cccgttttgt gtggttgttg ctcaggggat tgccggcgtt
    28801 ccggtgcttt ctggctccgg gttgcgcatc gtggtgtggg ttatcgccac tctgattggc
    28861 ctgatcttta ccatggtgta cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg
    28921 catgagtccg accgcttctt tcgtgaaaag caggcggatg ttgaacaacg tccgtttacc
    28981 tttggtgact ggctggtatt gattgtcctg acagccgtaa tggtctgggt gatttggggc
    29041 gtgatcgtta atgcctggtt tattccagaa attgccagcc agttcttcac catgggtctg
    29101 gtgattggca tcatcggcgt tgttttccgc cttaacggca tgacggttaa taccatggct
    29161 tcatccttta ccgaaggggc gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc
    29221 aaagggattt tgctgctggt tggtaatggt gaagcaggta atgccagcgt gttaaatact
    29281 atcctcaaca gcattgccaa tgccattagc ggtctggaca acgcggtcgc ggcctggttt
    29341 atgttgctct tccaggctgt atttaatttc ttcgtgacgt ccggttctgg tcaggcggca
    29401 ttaactatgc cgttactggc accgcttggc gatctggtcg gtgttaaccg tcaggttacc
    29461 gtgctggcgt tccagtttgg tgatggcttc agccacatca tttacccaac ctcagcttcg
    29521 ttaatggcaa cgctcggtgt gtgccgggtg gacttccgta actggctgaa ggtgggcgcg
    29581 accctgcttg gactgctgtt tattatgtcc agcgtcgtag tgatcggcgc tcagttgatg
    29641 ggctaccact aaaaatgtta agagccgcaa ttgcggctct tttttcattc tgccgtttca
    29701 gtctctacgg cttcattttt ggcattgcgc ttcatccaca acgccagcgc tttcagcgag
    29761 tctggagtga actcatcgca gcgtgcggtg atttcttccg gcgtcagcca gcaaactcca
    29821 ctgacttcat cttcctgtag ggcgaaggga ccgtgagaga cgcaactgaa caacgcgccc
    29881 cagacacggc aatttttatc ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg
    29941 gcaatgccca actcttcttc cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc
    30001 tgaactacac cgcctgcggt cgcatctaac atgccgggta aaaagtcttt tgtctcggta
    30061 cgacgctgga ccagaatttt gcccatgcca tcatgcacga cgatgtaagt tgcacgatga
    30121 cgcagacact gtgcccgcat ttgttcccgg ctggcttgtg caatgacttc gttctcttca
    30181 ttgacaatat ccacccattc agtacttgcc aaacgacgct gttccattat caggaaacct
    30241 tctttttctg gcgctcttac ggcgcatttt gagttgtggg taaattacgg attaatcgcg
    30301 acctgcgcaa tgatactttg atcattgagt gcgattacac taagtacgtc attatccagc
    30361 atgccataac ccgccggatt accgcctttc ggaatactca ccgagccggg gttgaagtgg
    30421 aaaatctccc cccgctgttc tgccactggc agatgggtat gaccgtacac cagcacatcg
    30481 ttctggttta aagcaggtag attttccggg ccaaaaagat gaccatgcgt caaaaacaga
    30541 cgttgttttt ccagtaatac ctgttgccac ggcgcggtta tcgggaaatg cagcatcatt
    30601 tgatccactt cgctgtcgca gttgccacgc acagcgataa ccttatgtgc cacttcatta
    30661 agccgttcag cgactttggc tggcgcgtaa ccctccggta aagcattacg cgggccgtga
    30721 ttcaacacgt cgccaaggat caccagccac tgggcaccgc tttggacaaa caactccaca
    30781 acacgttccg tcgccggtaa cgacccatga atgtccgatg caaacatcag tttcatcact
    30841 cactcctcgt cgaaaaaacg taagctatga tactggattc tgtcgttgct atcagccaga
    30901 ttgcttcgcc gaaagtgcaa taaaacgctg gacagacgct cgctgccact ggaatgtcgc
    30961 ataatccacc aggcgttctg gcacctcatc gccatgtagt accaggcggt taatcattag
    31021 cgccagatca gtatcagcaa tgcaccattc accaaataaa ttcggctgac ccagtgctaa
    31081 caaatgttct gccatcgcga acagtttctc tgcactggct tttccctcgg ccgttagtgg
    31141 cgcttttttc gcccccgcaa agacaacatc cgtcggacgc tcttcgcgga tgggcatcag
    31201 atcgctgcgc agccaggcct gaatctgtcg tgcacgcgca cgattttcta aatcaagcgg
    31261 ataaatacgt tcccaggtcg gtggcgcaaa tcgatcttcc agatactccg caatggcaga
    31321 agattcactc aactcaaaat catcgatttg taataacggc acacggcgtg tctgaccgta
    31381 accttgccac gtcggctgca aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa
    31441 cgacaggcct ttttcctgca acgccaccca ggcggataac acataagggg agaaaaagtg
    31501 ggcatctgac caaagcgtga tagcgggttt actcattatg tcctcggcaa taaagggggc
    31561 tggctatcaa actatagagc ctttttgtca ctgtcacgtg acgaatactc acgtaagtcg
    31621 ccgcgctttc tatacttgtg gtgttcagat gattaaacaa ccggagctgc aatgatcgat
    31681 ctctatttcg ccccgacacc caatggtcac aaaattacgc tgtttctcga agaagcagag
    31741 cttgattatc gcttgattaa ggtagacttg gggaaaggcg gtcagtttcg tccggaattt
    31801 ttgcgcattt cgcccaacaa caaaattccg gcaattgttg atcattctcc tgccgatggc
    31861 ggcgaaccgt taagcctctt tgaggctgct gccattttgt tgtatctggc tgagaaaaca
    31921 ggactctttt tgagtcatga aacgcgtgag cgcgccgcca cattacagtg gttattctgg
    31981 caggtaggcg gactggggcc gatgcttggg caaaatcatc attttaatca cgcagccccc
    32041 caaaccattc cttacgctat tgaacgttat caggttgaaa ctcagcgtct ttaccatgta
    32101 ctaaacaagc ggctggaaaa ctcgccctgg ctgggaggcg agaactacag cattgcggat
    32161 attgcctgct ggccgtgggt taatgcctgg actcgcctgc gaattgacct cgcaatgtat
    32221 ccggcagtca agaactggca tgagcggatc cgttcgcgcc ctgccaccgg gcaggcactg
    32281 ctaaaagcac aactcggtga tgagcgttcg gatagttaac agaaacaggt tctcgtgtat
    32341 tatttcatcc taagtaaaac aacggagaac ctgcaatggc acaacctgcc gctattattc
    32401 gtataaagaa ccttcgtttg cgtacgttta tcggaattaa ggaagaagaa attaacaacc
    32461 gtcaggatat tgttatcaat gtgacgatcc actaccccgc cgataaagcg cgcactagcg
    32521 aagatatcaa cgatgcgctg aattatcgca ccgtaacgaa aaacattatt cagcatgtag
    32581 agaataaccg tttctctttg ctggaaaaat taactcagga tgtgctcgat atcgcacgtg
    32641 aacatcactg ggtgacgtat gctgaagtgg agatcgataa actgcacgcg ctgcgctacg
    32701 ccgattcggt atccatgacc ttaagctggc agcgttaatc gccatatcgg gaggctgcat
    32761 gaacatagtg atcaccggag ggacgggatt aattggtcgc catttgattc cgcgtttgct
    32821 ggagctgggc catcaaatta cggtagtgac gcgtaacccg cagaaagcca gttccgttct
    32881 cggccctcgg gtgacactat ggcaagggct tgccgatcaa agcaacctca acggcgttga
    32941 tgcggtaatc aacctggccg gagaaccgat tgctgataaa cgctggactc acaagcaaaa
    33001 agagcgtctc tgccaaagcc gctggaatat cacgcaaaaa ctggtcgatt tgattaatgc
    33061 tagcgacacg ccgccgtcgg tactcatttc cggctcggca acgggctatt atggcgactt
    33121 aggtgaagtg gtggttaccg aagaggaacc gccgcataac gaatttactc ataaactctg
    33181 cgcccgctgg gaagagattg cctgccgggc gcaaagtgac aaaacgcgag tgtgcctgct
    33241 gcgaaccggc gtggtactgg caccggacgg cggtattctc ggtaaaatgc tgccaccgtt
    33301 tcgtcttggc ctgggcgggc cgattggttc cggtcggcag tatctggcct ggattcatat
    33361 tgatgatatg gtcaacggca ttctctggct gctggataac gagctgcgcg ggccatttaa
    33421 tatggtttcg ccctacccgg tacgcaatga acaatttgcc catgcgctcg gtcatgcgct
    33481 gcatcgcccg gctattttgc gcgtccccac aaccgccatt cggctgttaa tgggcgaatc
    33541 ttcagtactg gtattaggtg gacaacgcgc gctgcctaaa cggctggaag aagcgggttt
    33601 tgcgtttcgc tggtacgatt tagaagaggc gctggcggat gtcgttcgct gatgtggttt
    33661 acagcaaaca tccgccagtt aactccctgt gttacaggat tagtggcttt gcgcgataag
    33721 atcgtctggt gaaagtcggg tcaccatcat aactaactct ctgtctaaac ctctatccag
    33781 catctcctga gcaatacgca gggcttcttc gtgtttgccc tgcattgcgc cttcttcacg
    33841 taatctgtca gcaatggtca tcagtttctc cttttcttgt ggtgcgcgtt ctgttatctc
    33901 accaataaat gcacggaaac gctgggcatc ccctgtttgt aatacgtaat taaacagggc
    33961 ttttagctgt ctgtcattag tgttccctgt aactagcagc gaaacaattt ggtcgactaa
    34021 tcccaacaga tcgcgctgac gaatatgttt ctgaattaac tccaacagcg ccattttgcg
    34081 gtgttgcata atctcgtcat ccggcaccac ggtaatatcc accaacggaa acgctgatga
    34141 atatattttg cgggctatag caggctcggc aaattcatca agccagcaga gtgaataagg
    34201 ataaggactt ctgcaaccat gataaaacag catcgggatc accaatggaa gctctttata
    34261 gcccgcatca agatggtttt gcattgccgc aatggaataa cgcatcatgc gaaaagccat
    34321 taattcttcc ggcttacttt ggtgctctat cactacataa atataaccca ctccctcctg
    34381 cgttttcaca gaccacaaga ggtcggaata atattgccgc aggtcttcat caataaaact
    34441 gtttggttct agtttaagcg tcgttaaatc acacagtttg cgcagcggcg cgggaagatg
    34501 aatattaata aaattccgcg cggtgtctgg atggcgtaaa aaagatttaa ataccgcatc
    34561 atgcggcgtg gaagttgtcg atattgtcat ggcgttccgt cacccctcaa atttaagatg
    34621 acgcgacaat aaccacgatt aacacccaat gcagcaggca gtttttatct ttggacagcg
    34681 aattcagaag tatttttagc ggactgcaac ttttacattt cagcggaagc tgactcgcaa
    34741 attccccttg aaaatactcc ggcagcgtta gccaccggag aggaaattat ttcagcgatc
    34801 ccttaaggaa ctgttgcaga cgagggcttt gcgggttgcc aaataactgc tccggcgcgc
    34861 cctcttcttc tattttcccc tgatggagga aaatgacgtg agtagaaaca tggcgagcaa
    34921 agcccatttc gtgagtcact accaccatgg ttttcccctc ttctgccagt tgctgcataa
    34981 tacgtaacac ttcgcctacc agttcaggat cgagcgccga ggtaggttca tcaaacagta
    35041 aaacttctgg ttccatcgcc agcgcccgcg cgatagaaac acgctgttgc tgaccgccgg
    35101 aaagatgcac cggatatttc ccctgcgcac gttcgtctat cccgactttc gccagatact
    35161 tcaccgcccg ctcgcgcgct tcctgcttgc tcaggcccaa cacctgaatc ggcgcttcca
    35221 tgacgttttc caacaccgtc atatggctcc agagattgaa gtgctggaat accatcgtca
    35281 ggcgtgtgcg cagtaagcgc agttgatttt tatcggcgac tttgagttga ccgtctttgt
    35341 cgcgcaccag attgatcgtc tggccattga ccacgatcga tccttcactc ggtttttcga
    35401 ggaagttaat gcagcgcaga aaggtacttt tccccgatcc cgacgatccg atgatgctta
    35461 ttacatctcc ggcattcgct tgcagtgaga cccctttcag cacttcgtgt tcgccatagc
    35521 gtttgtgcaa atcgataacg tttaatttat tctcggacat cgtattctca gtgcgttgaa
    35581 gaaggtttca catgctgcaa ccagcgtttt tccgctctgc gaaagaggct gatcaggaca
    35641 taagagatga ttaaatagag caccgcggca atgccgaagg cggtaaaagg ttgatacgtg
    35701 gcggcgttaa tatcgcgggc tattttcagc agatccggca cagtggccgt aaacgccagc
    35761 gcagtagagt gcagcatcag gatcacttcg ttgctgtatg ccggtaacgc aatacgcagc
    35821 gcagaaggca aaataatgca gcgatacatt ttaaaggtcg agaagccata ggctctggcg
    35881 gcttcaattt ccccatgcgg aaccgaacgg attgccccag caaaaatctc ggtagtgtaa
    35941 gcgcaggtgt taagcgtcag cgccagcacg gtacagttca ggccactgcg gaagaaagcg
    36001 ttaaggaatt cggttccctt aacaatctca agcgtgtaca tgccggaata gaacaccagc
    36061 aactgaacat acagcggcgt accgcgaaaa atataggtaa ataaccagat tggaaactgg
    36121 atgtatttat tactggagac acgaccaatc gccagaaaca gcgccaggac tccgcctatc
    36181 actaccgaca aaataagcag ccacagagtg atcgccacac cagtaaagcg ataaccgtcg
    36241 gtccacagca gcggtttcca gtattcatgt aagatttcga tcacaggtca gccctcttca
    36301 cacccacgga gtagcggcgc tcaaggaaca gcagcacacc attggaaacg gtggtgaaaa
    36361 ccaggtaaat cacgccacag acgatggcga aatagaacgg ttcccaggta cttttgcctg
    36421 ccagctgcgt ggctttgacc acatcttcca ggccgagtaa cgaaaccagt gcggtagatt
    36481 tgaggatcac ctgccagttg ttgccaatgc ctggcagggc gtaacgcatc atcgccggaa
    36541 acatgatccg ccgaaacact tgcccgcgag taaaaccgaa cgccgtcgcc gcctctatat
    36601 gccctttcgg cactgccata aacgcgccac ggaacgtttc ggtaaaataa gcaccgtaga
    36661 taaaaccgag agtgataata ccagcgacca tcggatcgat atcaatctgc ccgacgccca
    36721 tcgcctccgt caccatgttt agcgcaatct gcaaaccata gaaaatcagc agcatcaaca
    36781 ccaagtccgg cacgcctcga atcagcgtgg tgtacccttc gaaaatcagc cccgaaagcc
    36841 gattttgcga gagcttaccg ccagcgccaa ttaaaccgat gattacagcg agcactacag
    36901 agctgatagc cagctccagc gtgacgagcg caccctgtaa aataacacct gaaaacccat
    36961 acaacatgct gcctgtcctg tcgtgtgtgg tgaattaccg cctcttgtct ccctccggtt
    37021 acccgaaggg agcgggcttt ctttcaccga tggcgattaa ccaccataaa catcaaaatc
    37081 gaagtacttt ttcgctaatt tctcgtaagt accgtcagcg cgcatttcgg caaaggcttt
    37141 gttcagtgcc tcgcgcagtt cgttatcttc tttacgcagg cccatgccgg tccctacgcc
    37201 aaacagtttt tcatctttaa cagacggacc accgaatttg taatctttac cgacgggttg
    37261 tttgaggaaa ccttcgctgg cagcgacctc atcctggaac gcggcatcaa tacgtccggc
    37321 agtcaggtca gaataaatgt tgtcctgccc ctgatacgag acgatttcaa tgccttttgg
    37381 tgcccagtgt tcattaccga acgtctcctg ggtggtgccc tgcaatacgc ctacccgttt
    37441 gcctttcagc gactcgactg tcggctgaat gtcagaattt ttcgccacca ccagacgaga
    37501 atcggcagcg tacagtttgt cggtgaaggc aatttcttgc tgacgttttt ccgtaatgga
    37561 aagcgatgac atgatggcgt caatcttctt cgcttttaag gacgggatta acgcatccag
    37621 cgggttttcg acaaacgtac attgcgtatt gatgcgcttg cacaactcct tcgccagctc
    37681 gatatcgaaa ccaaccagtt cgccttgtga attttttgat tcaaatggcg cataggtcgg
    37741 gtcggtaccg atgcggatgt tttgcggaat tgcagcaagt gccgcagttg cgctggagaa
    37801 ggccagaacc agagagagcg atagcaccag ttttttcata atgtcctcaa ctgacagtct
    37861 ttttataggg aatatttaca ggtttgttac acttatcgtg ccataaaatt gaccatcaag
    37921 gcaaaatatc ccgccccgtt agacgtatta tttttcatta aaaatgctta agtatgcatt
    37981 tcaatgcacc acgatagtgc aacaaacctg gcgtgcacca tgatgatcat tccatcaggt
    38041 acagcttccc agcgacgtat cagtcaccgt agacattaaa gtcgaaatac tttttcgcca
    38101 tcttgtcgta ggtgccgtcc tgacgcagct cgccaagcgc cttattgaag gcagccgtca
    38161 gttcagcata atctttacgt agccctacac cggtgccatc accgaagtat tttttgtctt
    38221 ttactgatga gccagcaaaa gcgaaatctt taccagcagg ttgcttgagg aatccttcgc
    38281 tggcagcaac ttcatcttgt aacgcagcat ccagacgtcc tgcagccaga tcggaataga
    38341 ccaaatcctg gttggcatag gccaccacat ccacgccttt actacgccag gtctcgttag
    38401 cgtaagcttc ctgggttgat ccctgcagca caccaacatg tttacctttc agtgaatcca
    38461 gcgttggctg aatcggtgaa cctttggcag caatcaaacg agaatctgcg gcgtacagct
    38521 tgtcggagaa ggcaatctcc tgctgacgtt tatcggtaat ggaaagcgac gaaataatag
    38581 cgtcgatttt tttcgctttc agtgagggga tcagcgcgtc aaagtcactg gcaacccagg
    38641 tacatttcac ctgcatccgt ttgcacatct cgttaccgag atcgatatca aagccaacaa
    38701 aatcaccttt agcatctttc gacgagaaca gcgcataggt ggtatcggtt ccgatacgta
    38761 ccgtctccgg tagcgccgca tagctggaag ccgctgcgga gagaccgact aataaagaca
    38821 gagcgagaat cgacttcttc atacataacc ctcaagtgaa aatggctttt ttatgttgtg
    38881 tattgtgttg tgtgtttgca ggctctttca tgcaggtctt atgccatctt gacgacaaca
    38941 gtaacattca acgttaaata tgttaataag acattgcatt attgtcctga agttgaagat
    39001 agcaggtata gcggttgaat cgcagcgttt cgattgaatg gcagaaacaa aatgtcgagg
    39061 atttgatcgc ggttgcaaaa ttgccctgaa acagggcaac agcggagtta tgcgccctgc
    39121 cagcgggcaa agagatcttc aggaagggtt attgcaaact ggtcaagaac acgattaacc
    39181 gtctgattta tcacatcatc aagggattgc gggcgatgat aaaacgccgg aacgggaggc
    39241 ataatcaccg caccgatttc tgccgcctga gtcattaaac gcagatggcc taagtgcaat
    39301 ggtgtttcac gcacgcagag caccaacggg cgacgctctt tcagcaccac atctgccgca
    39361 cgggtcagta agccatcagt atagctatgg acaatgccgg acagggtttt gattgaacag
    39421 ggtaaaatca ccatccccag cgtctggaaa gaaccggaag agatgctggc ggaaatatcg
    39481 cgcgcatcga gcgtgacatc agctaatgcc tgcacttcgc gcagggaaaa atccgtttcg
    39541 agggataagg tctggcgcgc cgcctggctc atcaccagat gcgtttcgat atctgtgaca
    39601 tcgcgcagaa cctgtaataa gcgcacgcca taaatcgcgc cgctggcacc gctgatgcct
    39661 acaatgagtc gtttcataaa tagttgccct gtcagacttg caggcagact ttgcagtatt
    39721 tcggcgggag ttgcaagtca gggtgccaga ccggcaccct cagcgaaggc atcatccttc
    39781 gttatgcatt tcgagatttt ccacttcgtt ctgacgttgc actgctttgg cgtcatcatt
    39841 acgtaacgta tcgaggaaat cgaggtagcc ctgatcaaca tctttggtga cgtagacgcc
    39901 gttgaacacc gagcattcaa actgctggat atccggattt tcagcgcgaa cggcgtcgat
    39961 cagatcgttc agatcctgga aaatcaaccc gtcagcaccg atgatctggc gaatttcatc
    40021 aacttcgcga ccgtgagcga tcagttccgt ggcgctcggc atatcaatac cataaacgtt
    40081 cgggaagcga atttccggtg ccgcagaagc gaggtacact ttcttcgctc cggcttcgcg
    40141 tgccatctcg ataatctgct cagaagtggt gccacggacg atggagtcgt cgaccagcag
    40201 gacgttttta tcgcggaact cggcgcggtt ggcattcagt ttacggcgca cggacttacg
    40261 acgcagctgc tggcccggca tgataaaggt gcggccaaca tagcggtttt taacgaagcc
    40321 ctggcggtac ggtttgccca gaatacgagc aatttccagc gcgatatcac acgaggtttc
    40381 tgggatcggg atcaccacgt cgatatccag atcttcccat tcgcgggcaa ttttctcgcc
    40441 cagtttcgtg cccatattca cacgcgcgct gtaaacggaa attttgtcga taaacgagtc
    40501 cgggcgggca aagtatacat actcaaacag gcacggattg ctgaccggat tgtcagcaca
    40561 ttgacgggta aacaactgcc cttcttcagt gatgtaaatc gcttcgcccg gcgcgacgtc
    40621 acgcaggaaa tcaaagccca gcgtatcgag cgctacgctt tcggaagcga ccatatattc
    40681 tgtacggttc tcgtcaatat cacgttttcc cagtaccagc ggacgaatcc cgtttggatc
    40741 gcggaaagca accataccgt ggccgataat catcgccaca caggcatacg cgccgcggat
    40801 taagcggttt gtggcagcaa tggcagcgaa aatattgtcg gcttccagcg ggtagtggcg
    40861 gaagttgtcc agctcgctgg cgaagatatt aagcagaatt tccgagtcgg aagtggtgtt
    40921 gatgtggcgg cgtttttctt caaacagttt tttacgcaac tcgtgagcgt tggtcagatt
    40981 gccgttgtgg gcaagcgtaa tgccatacgg ggagttaacg taaaacggct gcgcttcaga
    41041 ggcgctggag ctgccagccg tagggtaacg cacatgacca atgcccatat tgccctgcaa
    41101 acgctgcata tggcgagctt caaatacatc gctcaccagc ccgttcgctt tacgcaaacg
    41161 gaagcagtta ttggcatcta tggtgatgat gccggcggca tcctgaccgc gatgctgaag
    41221 caccgttaag gcatcataaa tcgactggtt aaccggcata acaccggcga taccgacaat
    41281 accgcacata cgtctttttc ctcgttaagc cacatctcag agcacttacg ctctgggcaa
    41341 gaaacttgac gagctttgca gataatcaaa aaaccatctg atgataaaac tgaattgcgg
    41401 gatcagctgt gatttgctcc agtcttcact tttcgacacc ccggtaaagg agtcgagaaa
    41461 gaagagaatg gcggcaacaa tcaacacacc gcgcaacgca ccgaaacaga cgcccagcac
    41521 ccgatcggtg cctgacaacc ccgttttctc caccaactgg cctatcacga agttcacgat
    41581 agcaccaacg atcagggtag cgataaacag taccgcgatg gcaatcccat ttcgaaccag
    41641 ttcgtcttca aagcccgtaa accagactga caggtaagtg tagtaatgac tggcaacaaa
    41701 gaaagcacaa ccccatgtca ccagcgataa cgcttcacga acaaagccgt ggatcaggct
    41761 aaccagagag gaaaaagcaa tcaccgcgat tatggcgtaa tcaatccaga ccatatgtgt
    41821 cccacgattt tacgccctgt catcctgttc ggggcgcatt ctaacagaaa aagaaaacgt
    41881 ttgcgtaggg atttccttcc cgcgcatcaa taaaaatggc gctgaaaaaa tattcaacgc
    41941 catcgacttt ttatgccttt gcggcatcgt gcaatgcgtg tcggatgcgg cgtaaacgcc
    42001 ttatccgacc tacggttcta acactgccca agcctgataa gacgcgccag cgtcgcatca
    42061 ggcaagactg tattaattcg gcgtatagcc cattaccacg ccacttaagc cagaaagttg
    42121 cttcaactca cccagcgaac ctttcagctt atctttcgag gcatccggcc caaccagaat
    42181 acgggtaatt ttaccctgca ctggcgtgga tggcgacgta taaacccgat aaccggcacc
    42241 gcgcagctta ccgacaatct cattcacttt atcggcattt ttcagcgcac ccagttgcac
    42301 aacataggct ttacccgttg gcgctgcttt ttcttccacg accggcttcg gttctggctt
    42361 cggcgcaggt ggtacttcaa cctttggttt aggcggctcc accggtttcg gcttcggtgg
    42421 ggcgaccggt gcaggttccg gttcaaactc ggtgttattg gctgcaatag tggccggatc
    42481 gagcgacggt gctgcggcat cacctgcccg cacctcttcc gctgcgcctt ccggcggctg
    42541 cgtcggcaac gcctgggtgg cggctggcat catatcaggc tcatcacgat cgcccgcttt
    42601 cggcaccagc gggatagccg cgaactcatc ctgataatgt tttttctgcc cgtccagcag
    42661 ccctggaagt acaatcaccc ccagcgccac cagcacgatc gtgcccacta accgattctg
    42721 aaacttactt gccaccgctt ctcctcgcgt caatcacttc catgacatgt gcgaccgtgt
    42781 ggaaagaacc acacaccagc acggtgtctt ccgctttagc gtccgccatt gcggcatccc
    42841 atgcctgcgc aacgctatca aatgatttgc cgttacccaa atgctcaagc agttgttctg
    42901 ccgtggcacc gcgcggccct tccagtggcg cacaatacca gtcatcaacc acgcttttca
    42961 accaggccag agttccggca atatctttat catgtagcat accgataacc gccagcacgc
    43021 gcccgttttt cggtagcgct ttcatacgcc cggtgagata ttccgccgca tgtggattat
    43081 gcgcgacatc aaaaataacg cgtggcgact cgctcacaat ctggaaacgt cccggcaaaa
    43141 ttgcgctggc aatcccgtcg cgaatggcat tttcactgac ttccagcccg ctggcacgca
    43201 gtgccgccag cgccgttgcg gcattcggtt gcgggacaag cggcaacggc agattttcca
    43261 gcgtgccgtg agcatcgcta aacgcccagt catgatcggt gacggaatag ttccactcaa
    43321 cgccccgacg ttgtaacagt gcaccttttt cctgcgccac atcagcaatg gtagaaggca
    43381 tttccggctc accgacaatt gccggttttt cgctgcggaa gatgcctgct ttctcgagac
    43441 caatactttc gcgatctgga cccagccagt cggtatgatc cagcgcaata ctggttacta
    43501 ccgcgacatc ggcatcgaca atattggttg cgtccagacg accgcccagc cctacttcca
    43561 gaatcaccac gtcaagttgt gcctgcttga acagccacaa cgccgacagc gtaccgtact
    43621 cgaaataggt cagggaaata tcaccgcgtg ccgattcaat ctccgcaaaa gaggcggtgt
    43681 gggccgattc cggcaattcc tggccctgca cacgtacgcg ctcggtataa cgcaccagat
    43741 gaggcgaact gtagacgccc actttgtacc ctgccgccat cagaatcgac tccagcgtac
    43801 ggcaggtggt gcctttgcca ttcgtacccg caacggtaaa cacaaatggc gctggtttca
    43861 ggacgccaag acgcgccgcg acctggctca cgcgctcaag gccgagatcg atagttttac
    43921 tgtgcaggtt ttccagataa gaaagccacg aagccagagg cgacgcggct tgaggagtgc
    43981 gtttgataat catggtatcc gctgattcgt tacggtgaga atagcaaaag ggcagagcca
    44041 gtggccctgc ccttatcagt tatcaggcct caggttcctg atccggtacc gggggtacca
    44101 ctacgccttc acgcggcgct tcaggattcg gcgctggcag attcatcaac ttcgccagaa
    44161 tgctcgccag tttcaggcgc atttccggac gacggacgat catgtcgatc gcgcctttct
    44221 cgatcaggaa ttcactgcgc tggaatctag gcggcagttt ttcgcgaacg gtctgttcga
    44281 taacacgcgg accggcaaag ccgattaacg ctttaggttc agcgatgttg agatcgccca
    44341 gcatggcgaa acttgcagaa acaccgccca tcgtcgggtc ggtcagcacg gagatgtacg
    44401 gcaagccgcg ctcctgcatt tttgccagtg ccgcagaggt tttcgccatc tgcatcagcg
    44461 acatcagtgc ttcctgcata cgtgcgccac cagaggcgga gaagcagatc agcgggcagt
    44521 tatcttcctg cgcctgctca acggcacgca cgaaacgtgc acccacaaca gaccccattg
    44581 aaccgcccat aaaggcgaac tcgaatgccg cagcgacaac cggcattcca tacagagtgc
    44641 ctttcatcac caccagcgca tctttttcgc cggtttcttt ctgcgcagat gccagacggt
    44701 ctttatactt cttggagtca cgaaacttca gctcatcttt cggctcaagc tcgctaccca
    44761 gctccacaag gcttccttca tctaacaggc tatgcaggcg attacgcgct gtcatacgca
    44821 tgtgatggtc acacttcgga cagacctcaa gattacgttc cagctcagcg cggtataaaa
    44881 cctgaccgca gctatcacac ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg
    44941 gagtaatgtt gcttttaatt cgttcaatcc agctcattag ggacctttct gtctgaacct
    45001 ggttcgatgc cagttttatc tttggggacg cataatgcca tttttgcccc caacagacca
    45061 tgaatgttgc acattaaaac ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg
    45121 cggagttact ttttattttg cggcgcgtgc cgcagcgcgt ttgtgacgga ttatttcgat
    45181 gacgcccggc aaaatagaaa ccacaataat cccgacgatc agcagcttaa ggttatcctg
    45241 aaccatcggg attgtaccga agaaatagcc tgcgtaggta aaaagcagta cccacaacag
    45301 tgcgccgatc acgttataag cagcgaaatg acggtacgac atgtggccca ttcccgcaac
    45361 aaacggggcg aacgttctga cgatcggcac aaaacgggcg agaataatcg ttttgccgcc
    45421 atgtttctca taaaactgat gggttttgtc gagataactg cgacggaaaa ttttcgaatt
    45481 gggattactg aataactttt caccgaacaa ccgcccgata gtgtaattga ccgcgtcacc
    45541 cacaatcgcg gcgatcagca tcagcactac catcatatgg acgttgagat cgttggtttc
    45601 cagtgatgcc aacgctccgg caacaaacag caatgaatca cccggtaaaa acggcattac
    45661 caccagaccg gtttcacaga acaaaattaa gaacaaaatg gcataaaccc agacgccgta
    45721 ctccgcgact agctctgcca gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata
    45781 aatcaggtcc atatttgcct atgccttgta ctcgtcatct ttcaggctgt aactacgttg
    45841 gctacagcct gaaatgttcc gagcattatt ctcgttagtc cgccagaaat agcgggccca
    45901 ttggcggttt tggaagatca taccggtcag ggtaatccac cgcgaccaga tacagccctt
    45961 ccgcttttgc cgttgctgcc gccagcgttc tgtcctttgc cgccagcaac tctgctatcc
    46021 agctctccgg ctggttgtgg gcaccgactt ccatcaggct gccgacaata ttcctgacca
    46081 tatgatgtac aaaggcattc gctttgatat caaccaccac ataaggaccg tgacgcgtga
    46141 cattaatatg cataacgttg cgccacgggg ttcgggactg gcactgcacc gcacggaacg
    46201 aggtgaaatc attctcgccc agcaagcatt gcgcagcccg atgcatccgt tcagcgtcca
    46261 gcggttcgta aaaatgggtt acccctttac tcagtaccgc cgggcgcagc cgatgattgt
    46321 agatgatgta gcgataacgg cgagccgtgg cgctaaatcg ggcatgaaaa tcatcaggta
    46381 cagctttaac ccaacgcacg gcgatgtcac caggtaaatt cgcatttacg cccaaggtcc
    46441 acgccgcgtc tttgcgctgc gcggttgttt cgaaatgcac cacttgcccg gtaccgtgta
    46501 cccctgcgtc agtacgcccg gcgcagaaga cggtgatggg ttcgttcgcc acctgggaga
    46561 gcgccttttc cagcttctcc tgcacactgc ggacttcatt ctgccgttgc cagccgtaat
    46621 acttactgcc gtcgtactca atgcccagcg caattttata aactggcagt tgttgctggt
    46681 cggacattag tacagatact cctgcaccag tttctcggcg atttttactg ccatcagcgc
    46741 gccgccaaag cgaacgttat cggccaccga ccagaactgg acttgctccg gcataccgta
    46801 gtcattacgc acgcagccaa cagaaagatg cggcgtaccc gaagcatccc ctacctgagt
    46861 cgggaattcg ttctcttcag agagcacaat atcttcgccc tgagcaaacg catcacgcgc
    46921 ttcttctgcc gccagcggac gcagtgcttc aaagttgacc atctgggcat gaccgtagaa
    46981 taccggtgcc tggacgacgc ttgccgaaat catcagccct tcgtcctgca ggattttgcg
    47041 tacttcgtca acgatacgac gttcttcacg cacgctacct tcgctatccg gcaacaacgg
    47101 cagcatattg aacgccagtt gacgaccgaa gaaatcttct tcgtcaatcg gaatgccgtt
    47161 gagcaatttc gcactctgcc ccgctaacgc atcgaccgct tttttgccct gggcggaggc
    47221 tgaaatcagg ctggtaacac tgatacgcga caaaccgccc tgatcgatta acggtttcag
    47281 tgccgccagc agctggctgg tcagactgtc tggtacggcg atgacattcc ggttgcggta
    47341 atcggtcagt acaaacgggt ttacttccgg caccaccagc ggtacgtcgg gttcgagagc
    47401 aaacagtccg ctgctgtcga tcaccaggca acctgagttg gtcgcttctt caacccaggc
    47461 agcggtagct tctttgcctg cgacaaaaaa tgccagctgc gcctgcgtcc agtcgaattc
    47521 agcggcatcc tgcacggtga ttgttttacc accaaagcgc agttgttcgc ctgcgctttc
    47581 gttacgtgcc agtgcataaa tttccccaac cgggaactga cgttcagcca gcgtttcaag
    47641 cagggcttcg cccatagcgc cagttgcgcc caggacggca atgttccagc cttcagacat
    47701 ggtggtttac tccagaaata gcaaagctcc ctgccaatgt tacagcagag agcatgaaga
    47761 agagattaac gtgccggatg atgaacggcg ttaaaaccca gtttacacag caatgatgcc
    47821 gcactggcgt catcacaaat tacatacaga gatgaccatt cacggcgctc aagatagttt
    47881 ttgcgcagtt tatcgaactc acccggtatc ccggcgactt tacgcagcgg tgcgtcatcg
    47941 cggcgcacat catacaccaa atgcaccagc cttttcagcg tcggttgatc gagcggacca
    48001 tgcagcgtaa tgcggccaaa ctcaggcgcg ggcagcaacg tatccagcgc aacgtgttgt
    48061 tcatgcccaa taaacttgct ataggcttca aacacttgcg tcgtgccacg cgctttaccc
    48121 tccagggtat agcctgcgat atgcggcgtg ccgatatcca cttttgtcag caattccaca
    48181 ttaagttccg gctcgccttc ccagacatcc agcaccacgc ttaacttctg gccttcattc
    48241 aggcaggtca gcagcgcggt attatcgacg actgcgccac ggcaggcgtt aatcagaatc
    48301 gctccgggct tcaggctacg gatcagtttt tcatccgcca gatgtagcgt tttgtacgga
    48361 ccatctttaa agagtggcgt atggaaagtc agaatatccg cgcgctggac taactcatcc
    48421 agcgagcgga aatcaccctc atccccacgg tcggcgcgag gcggatcgca aagtaaggtt
    48481 ttaatcccta acgcttccag tcgcgcctgc aatcgacgtc caacgttacc aacgcccacg
    48541 atccccaccg tacggtcgtg cagtgaaaat ccatcgcgtt cggcaagcat cagcagggag
    48601 gagaaaacat attccaccac cgcaatcgca ttacagccag gtgcagcgga aaaaccaatt
    48661 cccgcctgct tcaaccatgc ttcatcgaca tggtcggtcc ccgcagtggc agttccaaca
    48721 aatttaatgg gttttcctgc cagcaaagat tcattcactt tcgtgaccga acgcaccatc
    48781 agcgcatccg cgtctgccag ttgagcgacg gggattggac gcccgggaac cgcgatcacc
    48841 tcacccaaac ggctaaataa gtcgcgggca taaggcatat tttcatcaac aaggattttc
    48901 acgtttgtgt tacctgtatg agacgagagt taaccggaca agtatgccat aatctcgcgg
    48961 ccaggcatac ttgcgaagat ttcaggtata aggatacgta atgatacaac ctatttccgg
    49021 ccctcctcct gggcagccac caggtcaggg agataacctg ccgtctggcg cgggcaatca
    49081 gcctttatcc agtcagcaac gtacttcgct ggaaagctta atgacgaaag tgacctcact
    49141 gacgcaacaa caaagagcag aactgtgggc gggtatcagg cacgatattg gtctgtcggg
    49201 agattcaccg ctgctttcgc gtcacttccc tgccgctgag cataatctgg cgcaacgtct
    49261 gctggccgcg caaaaaagcc attctgcccg ccagctttta gcgcaattag gggagtattt
    49321 acgtctgggg aatcatcgtc aggcggtcac ggattatatc cgtcataact ttggtcagac
    49381 gccgctgaat cagctctcac cggagcaatt aaaaaccatt ctcaccctgt tgcaggaagg
    49441 gaagatggtt attccgcaac cacagcagcg cgaggcgacc gaccgtcctt tattaccggc
    49501 ggagcacaat gcgctaaaac agctggtgac caaacttgcg gcggcaacgg gggaacccag
    49561 caaacagatc tggcaatcga tgctggaact ttccggggtg aaagatggcg agttaattcc
    49621 agcgaaactg tttaaccatc tggtgacctg gctacaggcg cgtcagacgc taagccagca
    49681 aaatacgccg acgctggaat cactacagat ggcgctaaaa caacctttag atgccagtga
    49741 actggcggcg ttatcggcat atatccagca aaaatatggc ctttctgcgc aatcatcgct
    49801 ttcttctgcc caggccgagg atattcttaa tcagctttat caacggcggg ttaaagggat
    49861 tgatccgcgt gatatgcaac cgctgcttaa tccttttcca ccgatgatgg acacgttgca
    49921 aaatatggca acgcgtcccg cgctgtggat actgttagtc gcgattatcc tgatgctggt
    49981 ctggctggtt cgttaacccc gacgaaatga cagtatcgtg acaataatac ccagcaccgc
    50041 agagatcgcc ccggcaagaa ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc
    50101 cagcggcccg gagacgccga gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt
    50161 gccgcgaact tgtgagggga cgcgtttaac cacctccacg cccagcgcag gaaagataag
    50221 cgaacatccg gctccggtta acgccgcgcc cgctaatgcg acccatgcac ctggggcttg
    50281 ccagagcagc aacaagccca ccgtttctac aagcagagag acaatcgcca ctttcacgcc
    50341 gccaaaacgg tccggcatcc agccaaacat gacgcgcatc acgacaaatg cgccgccaaa
    50401 cgcggtaaga gtaaagcccg ccatcgccca tcctttgctg gcaaagtaga gcgaaacgaa
    50461 agtcccgata accgcaaaac caacgccttg tagtgccagc cctaaccctg gtttccagat
    50521 aagcccgaca acgctccaca gcgatggacg ttctcccgcc agggccggta ctttgcgcac
    50581 tgtgccgtta caggcccacg ccagtaaggg taataccatt gtggtgatcg ccagtgcggc
    50641 aaaaccgtaa tggctatgaa tcaacaggcc aagcggcgca ccaacagcga gggcaccgta
    50701 aatcgccatt ccgttccatg acatcacttt gccagagtgt tttggcccta cgatgcctaa
    50761 cccccaggtc agagcgcctg tcagtaactg gctttcacca aacccaagaa tcaaacgccc
    50821 gatgaccaac agggcaaatt tgaacggtgc ggagacaggc aaaatcgccg ccagcagcaa
    50881 cgcgccgcca gccagaccac aagctaacat cccctgaagc gccgaacgtt ttgcgccata
    50941 ttgatcggcc agtcgcccgg cgtaaccacg cgtcagcacc gtagccagaa actgaatccc
    51001 gacggcaatg ccgaccatgg tattgccata gcccagatca tgatgaacaa acagcgggat
    51061 aaccggcaac ggcaacccta cggtcatgta ggtgagaaaa accgcaaaag agatgcggaa
    51121 gagcgaaaaa ctggcagaag atcgtgtttc ggtttggctt acagcagtca tgcattactc
    51181 cagaatgcag cgcaaggcga ggagtatccc cgtctcatct ctctggtttc agggttacgg
    51241 tgcgttggca ggatttaacg cgtacgtctt ttcagaagga aatcgacaaa gcgggaagtt
    51301 tgcctagaac tggtggcgat tgtcaatgat gtgaaaaagg gaaccatcag gttccctttt
    51361 gcgttagtgc cggaggccgc attggcgcgt aacgtcggat gcgacgctgg cgcgtcttat
    51421 ccgacctact ccggcctact gcgaattaat cttttcagct tgcgcattac cagcgtggcg
    51481 ttggtgccgc cgaagccgaa gctgttagac ataacagtgg tcagttcgcg atcggtcgtt
    51541 tcggtcacga tgttcagacc cgcagcctgc tcgtccagct cttcaatgtt gatgctcggg
    51601 gcgataaagc cgtgttccag catcagcaga gagtagatag cttcctgcac acccgcagcg
    51661 cccagtgagt gaccggtcat ggctttggtt gcagaaatcg ccgggctctt atcgccgaac
    51721 acttcacgga tagctgccag ctctttcacg tcgccaaccg gagtcgaagt accgtgagag
    51781 ttcaggtaat cgattggggt atcaacgcca tgcatcgcca tctgcataca acgtactgcg
    51841 ccttcgccag acggagcaac catgtctgca ccatcagagg ttgcgccgta gccaacgatt
    51901 tcagcataga tgtaagcacc acgcgccagc gcgtgttcca gctcttcaac cactaccata
    51961 ccgccgccgc cagcgataac gaaaccgtca cggtgagcgt cgtaagtacg ggaggctttt
    52021 tccggggtgt cgttgtattt agtagatagc gcacccatcg cgtcgaattc gcaggccatt
    52081 tcccagcaca gctcttcgcc gccgccagca aacacgatgt cctgtttgcc cagttggatc
    52141 tgctctactg cgttaccgat acagtgtgcg gaagtcgcac acgcggagct gatggagtag
    52201 ttaacgccat gaattttaaa cggcgtggcg agacaggcag aaacgccgga tgccatcgct
    52261 ttagtgacca catacgggcc aaccgctttc aggccgcgcg ggccgcgcat tgcgtcagcg
    52321 ccgaacacct ggaaacgcgg ggaaccgccg ccggaacctg caatcaggcc aacgcgcggg
    52381 ttattctggt aagcttccgg agagaggccc gcatcagcga ttgcctgctc catagaaagg
    52441 aatgcataaa tggatgcgtc gctcataaag cgcacaactt tgcggtcaat gaggccagtg
    52501 gtatccagtt ttatgttgcc ccagacgtgg ctacgcatgc cggaatcctt cagctcctga
    52561 gagaaagtga tccctgaacg tccttcacgc agagatgcca ggacttcctg ctggttatta
    52621 ccgatgctgg aaacaatgcc caggccagta atcactgcac gtttcattca atacctctgt
    52681 aagtcgcaca tagagtaagt ttcgaatgca caatagcgta cacttgtacg ccgaacaagt
    52741 ccgatcagcc atttaataga gatatttgcg cagccttaca cacatcgcta agatcgagcc
    52801 accgcctgta agacgagtaa cttacgtgaa acactactcc atacaacctg ccaacctcga
    52861 atttaatgct gagggtacac ctgtttcccg agattttgac gatgtctatt tttccaacga
    52921 taacgggctg gaagagacgc gttatgtttt tctgggaggt aaccaattag aggtacgctt
    52981 tcctgagcat ccacatcctc tgtttgtgat agcagagagc ggcttcggca ccggattaaa
    53041 cttcctgacg ctatggaagg catttgatca gtttcgcgaa gcgcatccgc aagcgcaatt
    53101 acaacgctta catttcatta gttttgagaa atttcccctc acccgtgcgg atttagcctt
    53161 agcgcatcaa cactggccgg aactggctcc gtgggcagaa caacttcagg cgcagtggcc
    53221 aatgcccttg cccggttgcc atcgtttatt gctcgatgaa agccgcgtga cgctggattt
    53281 atggtttggc gatattaacg aactgaccag ccaactggac gattcgctaa atcaaaaagt
    53341 agatgcctgg tttctggacg gctttgcgcc agctaaaaac ccggatatgt ggacgcaaaa
    53401 tctgtttaac gccatggcaa ggttggcgcg tccaggcggc acgctggcga catttacgtc
    53461 tgccggtttt gtccgccgcg gtttgcagga agccggattc acgatgcaaa aacgtaaggg
    53521 ctttgggcgc aaacgggaaa tgctttgtgg ggtgatggaa cagacattac cactcccctg
    53581 ctccacgccg tggtttaatc gtacgggcag cagcaaacgg gaagtagcga ttatcggcgg
    53641 tggtattgcc agcgcgttgt tgtcgctggc gttattacgg cgcggctggc aggtagcgct
    53701 ttattgcgcg gatgaagccc ccgcgctggg tgcttccggc aatcgccagg gggcgctgta
    53761 tccgttatta agcaaacacg atgaggcgct aaaccgcttt ttctctaatg cgtttacttt
    53821 tgctcgtcgg ttttacgacc aattacccgt taaatttgat catgactggt gcggcgtcac
    53881 gcagttaggc tgggatgaga aaagccagca taaaatcgca cagatgttgt caatggattt
    53941 acccgcagaa ctggctgtag ccgttgaggc aaatgcggtt gaacaaatta cgggcgttgc
    54001 gacaaattgc agcggcatta cttatccgca aggtggttgg ctgtgcccag cagaactgac
    54061 ccgtaatgtg ctggaactgg cgcaacagca gggtttgcag atttattatc aatatcagtt
    54121 acagaattta tcccgtaagg atgactgttg gttgttgaat tttgcaggag atcagcaagc
    54181 aacacacagc gtagtggtac tggcgaacgg gcatcaaatc agccgtttca gccaaacgtc
    54241 gactctcccg gtgtattcgg ttgccgggca ggtcagccat attccgacaa cgccggaact
    54301 ggcagagctg aagcaggtgc tgtgctatga cggttatctc acgccacaaa atccggcgaa
    54361 tcaacatcat tgtattggtg ccagttatca tcgcggcagc gaagatacgg cgtacagtga
    54421 ggacgatcag cagcagaatc gccagcggtt gattgattgt ttcccgcagg cacagtgggc
    54481 aaaagaggtt gatgtcagtg ataaagaggc gcgctgcggt gtgcgttgtg ccacccgcga
    54541 tcatctgcca atggtaggca atgttcccga ttatgaggca acactcgtgg aatatgcgtc
    54601 gttggcggag caaaaagatg aggcggtaag cgcgccggtt tttgacgatc tctttatgtt
    54661 tgcggcttta ggttctcgcg gtttgtgttc tgccccgctg tgtgccgaga ttctggcggc
    54721 gcagatgagc gacgaaccga ttccgatgga tgccagtacg ctggcggcgt taaacccgaa
    54781 tcggttatgg gtgcggaaat tgttgaaggg taaagcggtt aaggcggggt aatctgctct
    54841 cgcagtgttt gccggatgcg gcgtgaacgc cttatccaac ctacgtgtga cgtgtaggcc
    54901 tgataagacg cgataagcgt cgcatcaggc atggtgctcc agacgccgca acttacagct
    54961 gtgacgcctg ctgaaacagg ttttcccaca tatcggtcac cagcgcctgg tcacgcggcg
    55021 acaattcacc ggcaccaatg gctttttcca gactctggct aacggtcgta tgcaccgcct
    55081 gagcggagtg gtcatcacca ctttccagtt ctgcgatggc taatgtcagg tggccacgca
    55141 aatacccact ggcaaacaac tcatcatcac tggcatggtc aaccatgccg tcgattaatg
    55201 ccagaatgcg tgattcaaac tccgcgatca tcttctttcc tcattgtaaa acgtggcgct
    55261 ggcttcagtt gagcgcttcc ggccacggga actgttccgc cgtaagttcc ggcgtgtgat
    55321 aataattttg tagtgcttta atgaagcgtg ccgggcgttc cggaatgcca tttgtcagat
    55381 aatccatcac ctgcgcatgt acccgccgtt gaaacaccac acgatccggt tcgaaatccc
    55441 cttccagatt gtcgcaactg acgttaaacg gatatccagc cgccacgcag aataaccagt
    55501 cgaacgcctg cggcttcact tcaacatctt caaactggct ttgcgtctgg gcatcgcgtc
    55561 cgtccgggca ataccagtag ccgaaatcaa ccagctcacg gcgcgctttt ccggcaatac
    55621 accagtgcga aatctcatga attgcgctgg cataaaagcc atgagcaaag acgattcggt
    55681 tatacggtac ttccgcatca gcaggaagat agatcggttc gtcgtcgcct ttaatcagac
    55741 gggtattaaa ttcatcggca aagcagctat taaaaatttc aatcaactgc tcgtagtggt
    55801 gtgtactgtt cattagttca tccccaacca gtggaggatc tcctgtccgt ggctatcgta
    55861 aagtaatttg gcactcatca ccgccgagac gataacaatc atcgggcgga tcagcttttg
    55921 tcctttgctc aacaccaacc gtgaacccat gcgcgcgccg aggaactgcc ccaccagcat
    55981 gacaaagccc gtcgcccaaa tcactttgcc gccgagaata aacagcagca gaccgccaat
    56041 gtttgacgtt gcgttgagta atttggcgtg agccgtggct ttggcgaggt tgaatccgca
    56101 cagcataacg aaggccagcg cgtaaaacga tccggcagcc gggccaaaga atccgtcata
    56161 aaaaccgacg cagccaccgg caattaacgc gaagggtaaa ccgtacatcc ggcgctggcg
    56221 atcttcttca cccagctttg gcatcagcaa gaaatagagg ccgatacaaa tcaccagaat
    56281 gggcaaaatc tgccgtaaga catcagcctg aacgtactgt accaacagtg cgccgctcat
    56341 tgagccgaca aaggtcatgg caatattgag tttctgatcg cttaaactaa ccactttgcg
    56401 gcgaataaag tagatagtgg cggaaataga gccgccgcag gcttgcagtt tattggttgc
    56461 cagcgcgtta gcgggagaca ttcccgcagc catcaatgcc ggaatggtga gtaacccacc
    56521 gccaccggca atcgagtcga taaatcccgc cagcatggcg acaaaaaaga gaactcccag
    56581 caacagcgga gaaaccataa acaggctatt aaacgtttcc attagatcac gtgctcatcc
    56641 agtagcgcct ggcaggaagg cggcaacgga ggcggtgtct tcttctcagg ctttgttgtt
    56701 cccggttttg gaggttcaaa ccagctttgc agttctgccc cgcaaccatc gcctgatggc
    56761 ggtaaaggtt gatcttcaca ctccagacta tcggcagggc aacgtaatcg tacatgcata
    56821 tgcgcgcgat gctggaacca gggtcgcact ttgcgcaacc agtcgcgatc ggtgcccgca
    56881 tcaaggcaaa gttgttgttt aatcgccgga ttaacaaaaa tgcgcgtgac gtctttgtcc
    56941 tgggcggcga gtttgatcaa gctgaaaatt tctggcttcc acagcgtgga gacaacgtgt
    57001 ttaccgtcgc gggaaactaa gtccagtgct tgcgggcgca agagctgcgc ggaggtccag
    57061 cgagttttcg gcagttgcag aaagatatcg acatccagtc cggtctggtg gctggcatga
    57121 ccgccgttga aacgcccacc agcgggcatc cccatatcgc caatcagcac cgtacccatg
    57181 cccagattgc tcacctggct actcagacgc tggataaaca tcaccagatc cgggtgaccg
    57241 aaatagcgac gctgatcggt acgcatgacc tgataatgtt cggactgtat cggcaacgtg
    57301 tcagcgccga caatacagcc attagaaaaa ctgcctatcg attgtgcgct acccggcaca
    57361 ggttgggtta ttttttgcca cggcgtcgct gccaggctag cactactggc aagcagagcc
    57421 agcagcgcaa tcgcggtttt gttcattttt taccagcgtg gaatatcagt cttcacatcg
    57481 gcattttgcg cccgctgccg taacaggtga tccattaaaa cgatcgccag catcgcttca
    57541 gcgatcggca ctgcacggat cccgacacag ggatcgtgac ggcctttggt gatcatctca
    57601 acttcttcgc caaagcggtt aatagtgcgc cccggcacgg taatgctgga ggtcggtttc
    57661 agcgccatat gagcaatgat ttgctgcccg ctgctgatac cgccgaaaat gccgcccgca
    57721 tggttgctct ggaaaccgtc tttggtgatt tcgtcgcggt tctggctgcc gcgcagcgcc
    57781 actacgtcaa aaccgtcgcc aatttccacg cctttcaccg cgttgatgct catcagcgca
    57841 tgggcgatgt cggcatccag gcggtcaaag accggctcgc caagtccggc ggggacacca
    57901 ctggcaacaa cggtgacttt cgcgccgatg gagtcgccct cttttttcag cgcgcgcatc
    57961 agttcatcta acgcgtcgat tttgtcgggg tccgggcaga agaacggatt ttgttcaacc
    58021 agcgaccagt ctttgatttc cagcggaatg tcgcccatct gggtcaggca gccgcggatt
    58081 tcaataccaa atttctcggc gagatatttt ttggcaatcg cccctgccgc cacgcgcatg
    58141 gcggtttcgc gggcggaaga acgtccaccg ccgcgataat cgcgcagacc gtatttttgt
    58201 tcgtaggtgt aatcagcatg gcctggacgg aagacgtctt taattgcgct gtaatcctga
    58261 gaacgctggt cggtattttc gatcaacaag ccaatgctgg tgccagtagt aacgccttca
    58321 aaaacaccgg agagtatttt gacctgatcc ggctcgcggc gctgggtggt atagcgcgat
    58381 gtcccagggc gacgacgatc aaggtcatgt tgcaggtccg cttccgtcag cggaatgcct
    58441 ggcggaacac catcgacgat gcagccgagc gccagcccgt gcgattcgcc gaaggtggtt
    58501 acgcgaaaga gttgtccaat tgtgtttcca gccatcacgg ctccgttatt gttgtgtttg
    58561 cgtgtttact taatctttat aaatcgcgaa atgttctcgt gcggcaataa gctgctcttt
    58621 agtgagcata aacacaccat cgccgccgtt atcaaactcc agccaggtga acggaacatc
    58681 gggatattgt tccataagat gtaccatgct gttgcccact tcacaaatca acacgccatc
    58741 atcagcaagg taatctgccg cgttaccgag aatgcgacgc gtcagtttca ggccgtcggt
    58801 gccagatgcc aggcccagtt ccggctcgtg acggtattcg tttggcaggt cggacatatc
    58861 ttccgcgtcg acatacggcg ggttagtgac aatcagatcg tattgcactt tcggcaaatc
    58921 acggaacaga tcggaacgaa tcggaatgac gttgtggatc agaccgtgtt cttcgatgtt
    58981 ctgttcggca accgccagcg cgtcgggaga gatatctacc gcgtcgactt ccgcttccgg
    59041 gaaggcataa gcacaggcaa tggcgatgca gccgctaccg gtacacatat ctaaaatatg
    59101 ctgcggttgc ttgctgatga gtccggcaaa tttattgttg atcagttcac caatcggcga
    59161 gcgcggcacc agcacgcgtt catcgacgta aaattcatga ccgcagaacc aggctttgtt
    59221 ggtcaggtaa gccaccggaa tgcgctcgtt gacgcggcgg atcacgcgtt caacaatacg
    59281 gtgtttttcg ctggaggtca gacgcgcggt gcgcatatct tccgggatat ccagcggcag
    59341 gtagagcgat ggtaacacca gctgtacggc ttcatcccac gggttatcgg tgccgtgacc
    59401 gtaccagata tttgccgcgc tgaagcggct taccgaccag cgcaatatgt cctgaatggt
    59461 ttgcagctca tttactgctt catcaacgaa aattttatcc acgtattcct ccagggcatg
    59521 atcgcaataa tttcggcggc tagtttgcca tgaagatgac gataaatcag cattcacgcg
    59581 cggtgagtga ggaaaaatac gtttaaaacg atcgattgtg ctacgagtcg ggtaaactgt
    59641 aggaaaatta gaaatagaga cagataaatg aaaaagaaaa caacacttag cgaggaggac
    59701 caggctctgt ttcgccagtt gatggcgggg actcgcaaga ttaagcagga cacgattgtc
    59761 caccgaccgc agcgtaaaaa aatcagcgaa gtgccggtga aacgcttgat ccaggagcag
    59821 gctgatgcca gccattattt ctccgatgag tttcagccgt tattaaatac cgaaggtccg
    59881 gtgaaatatg ttcgcccgga tgtcagccat tttgaggcga agaaactgcg ccgcggcgat
    59941 tattcaccgg aattgttttt ggatttacac ggtctgacgc agctgcaggc caagcaggaa
    60001 ctgggcgcgt tgattgccgc ctgtcgccgc gaacatgtgt tttgtgcctg cgtgatgcat
    60061 gggcacggga agcatatttt gaagcaacaa acaccgctgt ggctggcaca acatccgcat
    60121 gtgatggcat ttcaccaggc accgaaagag tacggcggtg atgcggcttt gttggtgttg
    60181 attgaagtgg aagagtggtt gccgccggag ttgccctgaa tgctggcagc aaatgccgga
    60241 gcagcgtaaa cgccttatcc ggcctacaaa tatgtgataa ttcaataaat tgcaattcac
    60301 ttcgtaggcc tgataagacg catcagagca atactacgaa cttcagttca ttcaatacat
    60361 actgaaaacg acacgtcgaa tcaatcggtt gcccactact ggtattcaca ttcaccacaa
    60421 tatcgtgtag tactttatcc gttaccggat gacgagttgg cgtaaccgtt accgttgatt
    60481 tatcacaacc gtaagaggta aaggttaaca attctgatac cgcgcggcag tatgctcctc
    60541 tgccatcatt atcagaaaaa ttactgccat cacagagtct gccgccttcc atcaggtagg
    60601 tcgaaagcgt ggtggtcact gttgaccccg tggactgaat tcgtaacgcc ttattatggc
    60661 tgacataatt ttcactccag gagatatcgt atgaagcgac cggtatcgtt gggcatattg
    60721 tgttatgcac tgtaggggcc gtatagcttc caaatccggg taaatctgta taggaataaa
    60781 aggatgcgat ccaagggcaa ccataatcat cagatgcaac ggtaatttta aacgtcatat
    60841 atgcacaacg aaaaattttc gtatctatgc cagggcaatt tgagttatta attgtatcaa
    60901 agtcagtcag taccaaagtt ggagctgaac ccgacccaga ccaggaaata acccgcaacg
    60961 tatcgccttt atacgcaaaa gaatcatatt tcccatatgc aacctccttt ggcgttatca
    61021 ccgtaacatt gaaaacactg ctcgctacag caccggttac cttgtccatg gcataataaa
    61081 ctctcacact gtcaaaactc gcactcataa gtttatctcc agcataactc ttagatgcgc
    61141 cgacaacact cagaacgagc agcattaggc tcgcgaaaat tatggtccat tttttcattt
    61201 cagaaatact caaaggttgc cgtcactgtg gcactaaagt ctccggacgt tacatctcgc
    61261 ccgctctttg cctgtaacca ggtattaaaa tttaggctgt tatttccctg attcagcggg
    61321 agcgtgacgc ctgttgtatt gttgattggc accgatgtgc ctgccgcagt ttcaatacca
    61381 atcccagcgc cggatgctga tgacgaggag tcaaaggcca gaaagccggg tagagcacta
    61441 tcttctgttc ctgtcaacgt tactttgaca ttaaacaatg tagaactatg acaatctttc
    61501 aattgaaaca ccaatggcac tcgctctgac tgcccaaggt tcattaaatc actggcggca
    61561 atggcaggaa aatgtacttc tgccaactgg ctaccattaa tcactggcgt acacgattta
    61621 ctgattaaat tgccggtaaa gtgcaaatta tctcctgcta ttgtggttcc actgcccagc
    61681 aacatcaggc tactaataaa aaacaacgta cgctttttct tcattggtaa tctacctgta
    61741 acgtggccca ggcctcaaag tccgcctctg gcagaatgac accactcttt tttactggca
    61801 cagccgttaa caccggtaaa tcggtttcat tcaccacaag tggcgtattg atcgtaaaac
    61861 tctgccctgc ctgtttgagc tgaatgccta aaccagtgat attgctgctt actgcagcat
    61921 tatcaaaatc gcttggtgtg ccactccagc tcaaggtcat ttccattaat gggtcgcgaa
    61981 ccgccgtatc gcaggtaatg gaatacggca cggtttgctc ataattgctg ccattaatgt
    62041 catcaataag cacatcctga aaagttattt caatggtttg agcgttgcta atcgtgcagt
    62101 taggcggctg cactaaataa ccgtgaaaag agacgtttga gagtgccatc gagcaaaagc
    62161 cccacagcaa aataagcgag atccgtttca ttggtaatcc accacaattg tggcactggc
    62221 gttaaattca gctgccgcta acgttgtacc actctgtttc accggcacag cttccagaac
    62281 gggaactccc gaggaaaaat taaatggcaa ccatgagcca gaggttaagt cgagtattgt
    62341 gtgatcacta cttttctgaa cccgaatacc aaacccagga atacccgtgc tgataactgt
    62401 ttcaccgtta atcacgaccg ttgtcgcctg catctgtaga cgcaggtcat ccattgccag
    62461 attgttacaa acaagcgagt agtcaatggg acgtttataa tcaaccctat tgatcttatt
    62521 aatgaataca ttaccaaact caactgccgc accatttacc gtacaaggag gaggggcatc
    62581 gacaagcacc cgtaaattca tacttactga cttactcgct gcaactgcaa agccgctacc
    62641 aacacaacat atcagtaacc cagttaccgc ccagacagaa caagatcctt tcatactggc
    62701 tctcccggcc tcagcctttg cggctctgct cttcatcgac ctagcaactg ttaccgttgc
    62761 aagtgaaaaa gagcggcatc cgagcgccat agtcgttgac ataggtcagt accggcacgc
    62821 tacccatctt cacattcagc ggtgcagagg ttttcggcga catcactatt ggcgagaaac
    62881 cggctgccgg attgccattt ttctgtgtgc tggcattact gaaaatgacg taatacgacg
    62941 tcgggttatt gacggtaaat tcctgaccgt tgcgggttaa agtaatctta tattgctagg
    63001 ggtttttcat gctgacgttc tccagcgcct tcggtcgcca gaacaactta atacgcgttt
    63061 gtagtgctat ttgcagcgtg ttggctttgt ttgattttgg cgggatctca cgcacgttga
    63121 agtagaacag gctttcacga tcggcaggca atttgttgat atccggcatt ccctgcactt
    63181 ttacctgccc gttcatcatt gaatcgatgc gttgcaccgg tggcagaaca gttaacggtg
    63241 aactgatctt gttacccttt tcatcttcga tccagctttg ggccagataa ggcagtttcg
    63301 gatcgttatt gcgcaacgtc acgctgattg atttgtcgct ttcattaaat atcaaacggg
    63361 tacgatctgg cgttacagaa gcctgggcag acaaactggc agcgctaagc agtaacgcta
    63421 gcgtcgtagc acccaatttg gcagagcaaa gtaaatcaga catggatcat tccttaatca
    63481 cgttattggt tagctgaaac agaaacaggg ggattggtgg gtgtcacctg ttgcgtctgc
    63541 tcctgaatga caggctttat gtcatcagga acaaccggcg cgacgttgcc tttatgctgg
    63601 catggcagta acaagccgtt aaacagatcg gcaggcagcg ggtccggcag gttgatatcg
    63661 caatgcgcaa caccactcca gaacacactc atgtgttcgc caggtttcac tccagccaga
    63721 taaacattgc cgtcatcatc gacaaggccc actgtctgct cgttatcatt ttttacttct
    63781 gcgccaaacg gaggatggct gccgtcttgt aaacgcagca ccgccatcgc tttttgacca
    63841 ctgatgacgg caaatttgcg gtagccaatc gccccttcag ttagcgtggc ttgtaccacc
    63901 gactgggttg cttcggcgtt ttccggcagt ttgttgaggt cgatatacgc ctgattgcgg
    63961 taatagttat tgacgtcaga aacgacggct ttaccaaaca tattggtata aacagcagca
    64021 ccgttacctt ctaccggaac atcggcaacg ccatcggcat caatcaacaa gcgtgtcccg
    64081 cccatattct gggtacggtg aagtgcgcca ccgtgggcag taagcgttgc gccgccctgt
    64141 aacgacaagc ccgcagaggt gtattgccct tcatggtagt tcgcactgag gtcaacctgc
    64201 gccagcgaac catcatggct gtaatagccg tcaacgctgg tgtgtttgtc actggtgccg
    64261 acgttcaact gatagtgagt cgcgtcatcg acacggctga aataaccgac ctgactgctg
    64321 tccgtcccac tgccatagta ccgttatagc taacggtact gttgtcgccc cacggcatac
    64381 tgagcgaaat gtacatgcct ttgtcggcct ggttgtcata ctcgtaacgg tagccagtca
    64441 tcgagatgct gacattacga atactgccca tattgaaata gtgcgagagc atgatgttgt
    64501 agtttgtctg ctcctcgcga tcccagtagg tatggcgggt atagttgaga taaaccgaaa
    64561 cacccgcatc gcggaagttc tggttataag tggcggtgta catctctttg tcgttgcccg
    64621 tgcgtaccat tccgctgtca ctggcatcca gatactcgct catggtcata aagttctctt
    64681 ccgagaagcg atatccagca aaagtaacgc ggctgttgag ctggtcaaag tctttggaat
    64741 aactcacacg gaaggagtta ccgtccagcg aacctttgcc ataagcggtg tctttatcca
    64801 gtttggtgtg cgagtgagta acatcaaacg cgaccgcgcc gaatgtagac aaatcgcgac
    64861 cgacaccaag cgccgcagac tgatagtttt catctcccag tgcgccgcca tataacgacc
    64921 agccgttagc gatcccccag gaagcttcag caccagaaaa aaattcaccc tcgacatggt
    64981 gtccccactc ttgcggacgg cccatcatga tcttatagcg aacctgacct ggacgagtga
    65041 ggtatggcat cgaggcggtg ctgatgtcat attcctgcac ctggccgttc tgttcttcaa
    65101 tgcgaatatg caacgtaccg gagacagaat caccaagatc ctgaatacga aacggtccag
    65161 ccggaacctg cgtttcgtaa atcacacgcc ccatctggct gacggtcact tttgctgtgg
    65221 tgtgtgccac gccggaaatg tctggcgcgt agccgcgcag attgggaggc aacatttgat
    65281 cgtcagtact gacactgcca ccaacatagt taaaaccatc aaaaatatcg gatctgaggt
    65341 aatcctcgcc cagcgccagt ttggctttta atgatggtaa cgcccgcctg gcatagtagc
    65401 gactccactc ccattttttt gagtttcatc gccgctgaat tcatcgtcat cattactgcg
    65461 agtatgttga tagttagtct gccagtcagc acgcatacgc cacggcccta ggttaacccc
    65521 aaccgtcccg ttgccgctga tctcgttact atcatcaccg ccattttctt cgtgccgggt
    65581 ttgtgcgttg atgctgtagt ccgcgacgat cccggagatg ccgtcatccc aacgtgaagg
    65641 cggatccctg tcgggataag tatattcgag gtaagcctgt ggcagtgaaa tgactaatgc
    65701 ggactggctt aaatcagcct taatttccat gccttccagt tgaccggatt tcaggcattt
    65761 agcatcgtgg ctccattgca gatttttcgc cacgtcttct tttaaaccaa actgcgctac
    65821 cagttccggt gtcagacaag catagctttt gctcgcgtca tcttcaccag cataccagta
    65881 aatatcgtac tcttccgcca atggctgttt atttagttga acctgtaaat tgtattttcc
    65941 gggctcaaca tatccctggc tggaaaaacg cttcatatca atttttgtgt cgccttttaa
    66001 ttccagaaaa cgggaatcaa actgaatgtc gtcttcagcc cagacactgc tataactccc
    66061 tgacattgcc agcgcaatgc accacggaag aatccgtaat cgaaaaagag aatggtcagg
    66121 catacacgtt tctatccgtt tttattcttg ttcggtcaaa atacatttaa atcccccata
    66181 gcagtgtcgc tatgggggta tgccttacag gtaagtaatc tggaaggtgg tatttgcttc
    66241 aaaattaccc agatctggcg catcagctgc gccgaccagc caggcgttaa agttcagtgt
    66301 ctgcttcgct ttgcctttgt tggtagaagt gtctttcact attttctggt caatacccat
    66361 gccgcttttg taagaggtac ccagagagtc accgatcgcc aggctaacat tgttaaatgc
    66421 cgcaccggta tcggtattga aaatggtgta ataattgccg ctatttgcag aagaaacggt
    66481 accagtaaag gtagtggtca ttgtttcctg agtatcaaat acgcaatctt gcagacgaat
    66541 ctggaaactt ttcggcgtgg tggtgccgtt atttttcagt ttttcggcac cgatatcacc
    66601 catatcaact tccagtttgt gatcgtccgg aacgatagaa cacactgagt cttcaatggt
    66661 gccgtaaaaa cgtgcagtac cgttgttacc cgcagcgatt gttgccgtag aggtgaacac
    66721 gcccatcacc attgccgcag caatagctgt tttaacaaac ttactcatta tttgtcctta
    66781 aactataaat aattaaaaat ataaacattg caatacattg attcagtcaa tagccaatgt
    66841 ttatacccag ccattgaata cggcatgcga cccaaaaaga acaggtcgta gaaaattgta
    66901 ttcattggtg tggggccgca tagtgatgtt tccacacccc tgatccaatt taattttact
    66961 aaagagtaaa tgtagattta attaatatat tgacagaaga aaggttatct taaggac
//
LOCUS       DAJTYM010000006        65668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTYM010000006 DAJTYM010000000
VERSION     DAJTYM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..243)
                     /gene="folK"
                     /locus_tag="OO575_000409"
     CDS             complement(<1..243)
                     /gene="folK"
                     /locus_tag="OO575_000409"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6663502.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQ"
     gene            complement(240..1658)
                     /gene="pcnB"
                     /locus_tag="OO575_000410"
     CDS             complement(240..1658)
                     /gene="pcnB"
                     /locus_tag="OO575_000410"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6663503.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(1697..2623)
                     /gene="gluQRS"
                     /locus_tag="OO575_000411"
     CDS             complement(1697..2623)
                     /gene="gluQRS"
                     /locus_tag="OO575_000411"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6663504.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2660..3115)
                     /gene="dksA"
                     /locus_tag="OO575_000412"
     CDS             complement(2660..3115)
                     /gene="dksA"
                     /locus_tag="OO575_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6663505.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3293..3997)
                     /gene="sfsA"
                     /locus_tag="OO575_000413"
     CDS             complement(3293..3997)
                     /gene="sfsA"
                     /locus_tag="OO575_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6663506.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4012..4542)
                     /gene="thpR"
                     /locus_tag="OO575_000414"
     CDS             complement(4012..4542)
                     /gene="thpR"
                     /locus_tag="OO575_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6663507.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4616..7045
                     /gene="hrpB"
                     /locus_tag="OO575_000415"
     CDS             4616..7045
                     /gene="hrpB"
                     /locus_tag="OO575_000415"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6663508.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7241..9775
                     /gene="mrcB"
                     /locus_tag="OO575_000416"
     CDS             7241..9775
                     /gene="mrcB"
                     /locus_tag="OO575_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6663509.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            9995..12238
                     /gene="fhuA"
                     /locus_tag="OO575_000417"
     CDS             9995..12238
                     /gene="fhuA"
                     /locus_tag="OO575_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6663510.1"
                     /translation="MARSKTAQPKQSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12289..13086
                     /gene="fhuC"
                     /locus_tag="OO575_000418"
     CDS             12289..13086
                     /gene="fhuC"
                     /locus_tag="OO575_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6663511.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13086..13976
                     /gene="fhuD"
                     /locus_tag="OO575_000419"
     CDS             13086..13976
                     /gene="fhuD"
                     /locus_tag="OO575_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6663512.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            13973..15955
                     /gene="fhuB"
                     /locus_tag="OO575_000420"
     CDS             13973..15955
                     /gene="fhuB"
                     /locus_tag="OO575_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6663513.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(15990..17270)
                     /gene="hemL"
                     /locus_tag="OO575_000421"
     CDS             complement(15990..17270)
                     /gene="hemL"
                     /locus_tag="OO575_000421"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6663514.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17495..18916
                     /gene="clcA"
                     /locus_tag="OO575_000422"
     CDS             17495..18916
                     /gene="clcA"
                     /locus_tag="OO575_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6663515.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            18940..19005
                     /locus_tag="OO575_000423"
     CDS             18940..19005
                     /locus_tag="OO575_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663516.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            18998..19342
                     /gene="erpA"
                     /locus_tag="OO575_000424"
     CDS             18998..19342
                     /gene="erpA"
                     /locus_tag="OO575_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6663517.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19389..20012)
                     /locus_tag="OO575_000425"
     CDS             complement(19389..20012)
                     /locus_tag="OO575_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6663518.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20050..20850)
                     /gene="btuF"
                     /locus_tag="OO575_000426"
     CDS             complement(20050..20850)
                     /gene="btuF"
                     /locus_tag="OO575_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6663519.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20843..21541)
                     /gene="mtnN"
                     /locus_tag="OO575_000427"
     CDS             complement(20843..21541)
                     /gene="mtnN"
                     /locus_tag="OO575_000427"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6663520.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21625..23142
                     /gene="dgt"
                     /locus_tag="OO575_000428"
     CDS             21625..23142
                     /gene="dgt"
                     /locus_tag="OO575_000428"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6663521.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23272..24696
                     /gene="degP"
                     /locus_tag="OO575_000429"
     CDS             23272..24696
                     /gene="degP"
                     /locus_tag="OO575_000429"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6663522.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24851..26007
                     /gene="cdaR"
                     /locus_tag="OO575_000430"
                     /pseudo
     CDS             24851..26007
                     /gene="cdaR"
                     /locus_tag="OO575_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26096..26482)
                     /locus_tag="OO575_000431"
     CDS             complement(26096..26482)
                     /locus_tag="OO575_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6663523.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26796..27620)
                     /gene="dapD"
                     /locus_tag="OO575_000432"
     CDS             complement(26796..27620)
                     /gene="dapD"
                     /locus_tag="OO575_000432"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6663524.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27651..30323)
                     /gene="glnD"
                     /locus_tag="OO575_000433"
     CDS             complement(27651..30323)
                     /gene="glnD"
                     /locus_tag="OO575_000433"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6663525.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30385..31179)
                     /gene="map"
                     /locus_tag="OO575_000434"
     CDS             complement(30385..31179)
                     /gene="map"
                     /locus_tag="OO575_000434"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6663526.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31547..32272
                     /gene="rpsB"
                     /locus_tag="OO575_000435"
     CDS             31547..32272
                     /gene="rpsB"
                     /locus_tag="OO575_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6663527.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32530..33381
                     /gene="tsf"
                     /locus_tag="OO575_000436"
     CDS             32530..33381
                     /gene="tsf"
                     /locus_tag="OO575_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6663528.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33528..34253
                     /gene="pyrH"
                     /locus_tag="OO575_000437"
     CDS             33528..34253
                     /gene="pyrH"
                     /locus_tag="OO575_000437"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6663529.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34545..35102
                     /gene="frr"
                     /locus_tag="OO575_000438"
     CDS             34545..35102
                     /gene="frr"
                     /locus_tag="OO575_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6663530.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35194..36390
                     /gene="ispC"
                     /locus_tag="OO575_000439"
     CDS             35194..36390
                     /gene="ispC"
                     /locus_tag="OO575_000439"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6663531.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36579..37337
                     /gene="ispU"
                     /locus_tag="OO575_000440"
                     /gene_synonym="uppS"
     CDS             36579..37337
                     /gene="ispU"
                     /locus_tag="OO575_000440"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6663532.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37350..38207
                     /gene="cdsA"
                     /locus_tag="OO575_000441"
     CDS             37350..38207
                     /gene="cdsA"
                     /locus_tag="OO575_000441"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6663533.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38219..39571
                     /gene="rseP"
                     /locus_tag="OO575_000442"
     CDS             38219..39571
                     /gene="rseP"
                     /locus_tag="OO575_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6663534.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39601..42033
                     /gene="bamA"
                     /locus_tag="OO575_000443"
     CDS             39601..42033
                     /gene="bamA"
                     /locus_tag="OO575_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6663535.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42155..42640
                     /gene="skp"
                     /locus_tag="OO575_000444"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42155..42640
                     /gene="skp"
                     /locus_tag="OO575_000444"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6663536.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42644..43669
                     /gene="lpxD"
                     /locus_tag="OO575_000445"
     CDS             42644..43669
                     /gene="lpxD"
                     /locus_tag="OO575_000445"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6663537.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43774..44229
                     /gene="fabZ"
                     /locus_tag="OO575_000446"
     CDS             43774..44229
                     /gene="fabZ"
                     /locus_tag="OO575_000446"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6663538.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44233..45021
                     /gene="lpxA"
                     /locus_tag="OO575_000447"
     CDS             44233..45021
                     /gene="lpxA"
                     /locus_tag="OO575_000447"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6663539.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45021..46169
                     /gene="lpxB"
                     /locus_tag="OO575_000448"
     CDS             45021..46169
                     /gene="lpxB"
                     /locus_tag="OO575_000448"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6663540.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46166..46762
                     /gene="rnhB"
                     /locus_tag="OO575_000449"
     CDS             46166..46762
                     /gene="rnhB"
                     /locus_tag="OO575_000449"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6663541.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46799..50281
                     /gene="dnaE"
                     /locus_tag="OO575_000450"
     CDS             46799..50281
                     /gene="dnaE"
                     /locus_tag="OO575_000450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6663542.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50294..51253
                     /gene="accA"
                     /locus_tag="OO575_000451"
     CDS             50294..51253
                     /gene="accA"
                     /locus_tag="OO575_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6663543.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51352..53493
                     /gene="ldcC"
                     /locus_tag="OO575_000452"
     CDS             51352..53493
                     /gene="ldcC"
                     /locus_tag="OO575_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6663544.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53550..53939
                     /locus_tag="OO575_000453"
     CDS             53550..53939
                     /locus_tag="OO575_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6663545.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54004..55302
                     /gene="tilS"
                     /locus_tag="OO575_000454"
     CDS             54004..55302
                     /gene="tilS"
                     /locus_tag="OO575_000454"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6663546.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55351..55611)
                     /gene="rof"
                     /locus_tag="OO575_000455"
     CDS             complement(55351..55611)
                     /gene="rof"
                     /locus_tag="OO575_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6663547.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55598..55798)
                     /locus_tag="OO575_000456"
     CDS             complement(55598..55798)
                     /locus_tag="OO575_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6663548.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55964..56509
                     /locus_tag="OO575_000457"
     CDS             55964..56509
                     /locus_tag="OO575_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6663549.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56506..56928
                     /gene="arfB"
                     /locus_tag="OO575_000458"
     CDS             56506..56928
                     /gene="arfB"
                     /locus_tag="OO575_000458"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6663550.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56942..57652
                     /gene="nlpE"
                     /locus_tag="OO575_000459"
                     /gene_synonym="cutF"
     CDS             56942..57652
                     /gene="nlpE"
                     /locus_tag="OO575_000459"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6663551.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(57807..58631)
                     /locus_tag="OO575_000460"
     CDS             complement(57807..58631)
                     /locus_tag="OO575_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6663552.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58684..60402)
                     /gene="proS"
                     /locus_tag="OO575_000461"
     CDS             complement(58684..60402)
                     /gene="proS"
                     /locus_tag="OO575_000461"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6663553.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60513..61220)
                     /gene="tsaA"
                     /locus_tag="OO575_000462"
     CDS             complement(60513..61220)
                     /gene="tsaA"
                     /locus_tag="OO575_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6663554.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61217..61621)
                     /gene="rcsF"
                     /locus_tag="OO575_000463"
     CDS             complement(61217..61621)
                     /gene="rcsF"
                     /locus_tag="OO575_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6663555.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61739..62554)
                     /gene="metQ"
                     /locus_tag="OO575_000464"
     CDS             complement(61739..62554)
                     /gene="metQ"
                     /locus_tag="OO575_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6663556.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62594..63247)
                     /gene="metI"
                     /locus_tag="OO575_000465"
     CDS             complement(62594..63247)
                     /gene="metI"
                     /locus_tag="OO575_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6663557.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63240..64271)
                     /gene="metN"
                     /locus_tag="OO575_000466"
     CDS             complement(63240..64271)
                     /gene="metN"
                     /locus_tag="OO575_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6663558.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64459..65034
                     /gene="gmhB"
                     /locus_tag="OO575_000467"
     CDS             64459..65034
                     /gene="gmhB"
                     /locus_tag="OO575_000467"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6663559.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65398..>65668
                     /locus_tag="OO575_000468"
     rRNA            65398..>65668
                     /locus_tag="OO575_000468"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15516 a        16271 c        18129 g        15752 t
ORIGIN      
        1 ctgttgcagt tctatgcgct gtgtatggtt gagtaactct tccggggcgg gcgaagtttc
       61 aagcgccacg gcggcgttga ggtaatcggg ttgatcttgc ggccccagcg gtggggtgcg
      121 gtaaaacgaa gaaacggcaa gaatgcggct ttcagggata tcgcctaatg ctttcagggc
      181 agcattgacc tgctccagcg gagaggccag attgctgcct atggcaatat acgccactgt
      241 catgtggtac cctcacgacg cggtgcgcgt ttgcgtggac gacgagtacg acgacgcggt
      301 gacggttctt catccagctc gttaagcatc cctttttgat ctggtggtgc ggaaacctgg
      361 aactcacccc accatttcac cagacgctgc agttcagcat tacgttcaac ttcagctcgc
      421 aaggccaaca ggtcataagc cgcacggaac ttaggatgct ccagcagttt ccatgcgcgt
      481 ttaccctgac gacgggacat acgcaactgc aactgccaga tatcgcgggt taacgtcgtc
      541 agacgtttcg ggattgccag tgaacggcag gcttcgtcca gcacgtcgtt catcgccagc
      601 gcgaaagcgt cgtgataggt caggccgctt tcctgggcga tcttctgtgc cgtctccagc
      661 agtgggtacc agaacatggc ggcaaacaag aacgccgggt tcacgcgcat atcgttatgg
      721 atacgcgtat cggtattctt cagcacctgt tcaatgatcc gctccatcgg gctgtcgcca
      781 ttttccgtga agtagcgggt aatggtcggg aacagcggct ggaacagatg atattcacac
      841 aacagcttat aggtttcgta accgtagccc gcttgtagca gtttaagcga ttcttcaaac
      901 aggcgtgccg gtgggatatc gttcagcagg gtagcgaggc gagggatcgg ttctgcggtt
      961 tccgggctga tgcgcatacc caatttggcg gcaaaacgta ccgcgcgcag catacgtacc
     1021 ggatcttcac ggtagcgcgt ttccgggtta ccaatcagac ggataacgcc gtccttcaga
     1081 tccttcatac caccaacgta atcacggacg gtaaaatccg ctacgctgta atacaggctg
     1141 ttgatagtga aatcgcggcg ctgggcgtct tcttcgatgg agccgaaaat gttgtcgcgc
     1201 agcaacatgc cgttttgccc gcgttgggag gtcgtgcggt cgctgacgtt accttcgtgg
     1261 tgtccacgga aggtcgcaac ttcgataatc tccgggccaa acattacatg agccagacgg
     1321 aaacggcgac ccaccaggcg gcagttacgg aacagtttgc gcacctgctc aggcgtggcg
     1381 ttagtggtta cgtcaaaatc tttcggcttt ttgccaagta acaggtcgcg cacgccgccg
     1441 ccaaccagcc aggcttcgta tcccgctttg ttgagcctgt acattacctt cagggcattt
     1501 tcactgatat ctttgcggga aatagcatgc tgctcacgcg ggatcaccgt cacctgtgga
     1561 cgggcgactg cctgttcagc ctcgctttcc tcgcggctta gcaccttgcg gcaaaaatta
     1621 gcgactcggg taaaaatagt acacctcggt agtgtcaaac atcattcagg acaaaaaaat
     1681 agcggctaat catagctcag catgacgcat ttgagaatgt tgaatttaca attgccgact
     1741 cgggcacggc ggtcagcatc cagtttttga cggctgactg aaggatttgc tcgacgctga
     1801 aatcctgcca gtgtgcttct gcctgctgcc ccagaaattg aagtgccgcg attagtaccg
     1861 ggcgtggatc gcctttcggc aacgcaggcg catgattctg cttggaaagt ttagcgcctt
     1921 gtggattaag cgccagcggc agatgaatgt aatctggcac tttccagcca aaaagctggt
     1981 acagcgggat ttgccttact gttggttcaa tcagatcagc cccacgcact atttctgtaa
     2041 cgccctggaa atgatcatca accacaacag ccaggttgta ggcgaacaac ccatcacggc
     2101 gatgaatgat aaaatcttcc cgtgccagtt tttcgtcggc gtgaataatg cctcgcagca
     2161 ggtcagtaaa ttgcgtgacc ggatgctgct ggcggatacg cactgcggcg ttgtctggtc
     2221 catgatgcaa cacccggcaa tgaccgtcgt aaataccgcc aatgctttga atacgcgcac
     2281 gcgtacaggt gcagtaataa cttagtcctt gttcatgtaa ccaggcgagt gcttcacgat
     2341 aggcgtcgtg acgttgcgat tgccagagaa catcgccgtc ccagtgcaga ccgtaatgtt
     2401 ccagctggcg caggatagtt tctgcggcac cgggaacttc acgaggcggg tcgatatctt
     2461 ctatgcgtac cagccagcga ccttgccggg cgcgagcctg caaatagctg ccgagcgcgg
     2521 cgatcagaga gccaaaatga agctcgccgg aaggagaggg ggcgaagcgg ccaatatact
     2581 gtgtgtctgt catctctttg aacaaaaaat aaggcgggag catttcccgc ctgtggtaaa
     2641 cgtgatggaa cggctgtaat tagccagcca tctgtttttc gcgaatttca gccagcgttt
     2701 tgcagtcgat gcacagatcg gctgtcgggc gcgcttccag acggcgaata ccaatttcaa
     2761 caccgcagga ttcgcagtag ccgaaatctt cgtcttccac ttttttcagc gtcttctcga
     2821 tctttttgat cagcttacgc tcgcgatcgc ggttacgcag ttcgaggctg aactcttctt
     2881 cctgggctgc acggtctacc gggtccggga agttggctgc ttcatcctgc atatgtgtaa
     2941 cggtgcgatc gacttcatcc ctgagttgat tacgccatgc ttccagaata cgacggaagt
     3001 gcgccagctg ggcttcattc atatactctt cgcccggctt ctcctgatat ggttccaccc
     3061 cagcgatggc gagaatactc agggacgatg ttttacggtt ttgcccttct tgcatgttgc
     3121 ttctccttaa cacgcactat cgatccccat gttcggggga aaaatgaggc cgctataaat
     3181 agcagatgct tttccggata gcaattatct aaacgtaaca cttgacaact gtgtgaggaa
     3241 aagcgtattt gcgcacgcga ccagaatgta aattaaccag ttacttactt tactacaatg
     3301 taaccggcag tgatttttta agagccatgc cttcagcaga aatttccgct ttgtaagcca
     3361 gaatttctac ccccctctgt tgagcttctg acaatagttg cgcgtatttc tcatcgatgt
     3421 ggcgcgcggg tgaaaaccgt gtaatggctg aatgcagcac ggcgaaaaaa ataaccgcac
     3481 gctggccttc agccgctacg ctcatcaact cccgaaggtg tttctgacct cgttcagtga
     3541 ccgcatcggg aaaatatccc tgttcgttct ccgctaacgt aaccgatttc acttcaatat
     3601 agcagtctgg acgcgaatcc gcctgcaaca taaagtcaat acggctgcgt tcggagccgt
     3661 attttacttc gcttttcagc gagctatagc ctgacagttc tgaaattgat tcattaagga
     3721 tagcctcttt cgtcaacctg ttagcccaaa gcgtattgac gcaaataaat gcgccgctct
     3781 ggctttgagt taattcccag gtgtgtgggt atttccgttt ggtgttgtct gaagtcgaat
     3841 accagacggt atcgccaggc gttgcacaac cggtcatcgc acccgtattc gggcagtgta
     3901 gcgttaattc gcgaccatcg ggtgtgatca catcggctaa aaaacgtttg taacgctgaa
     3961 ttagcgtcgc gcgctgtaga gggggagaaa attccattgc gacaatcctt gttattgcgt
     4021 tagcgcccag cgttttagcg gcgtgtagcg tgtgcgtcca cgggcaaacg aggaggcgta
     4081 aagggtgaac tccgtcaccg cataagacca gttaaaaccc ggcggcggga atgtcaccgc
     4141 ctcgctggcg tcgcgcaata aggtaatatg tggatgaaac ggacgattgc tttgaaaaca
     4201 accgctgcgg gcagcctgtg aacggagcat attcgccagc tggattaagc cgcgtggagg
     4261 ctgacgcatc cctaaccaca ccacacgcga acgcagccat tgtccggcgt catcaagcgt
     4321 gagtgtgaaa ccaggttgac gaatccgtcc ggctaaaaga gaaagcgcct tctctttctc
     4381 tgcgctcact tcgcctaaaa atgccagagt caggtgcaaa ttatcggcgg cgaccggacg
     4441 tcccgcctca ggtgggaagt gtgtggcgcg ccaatggata atctgttcgc ggatttctgc
     4501 aggtaagtcg atagcaaaga acagacgttg cggttcagac atgtgaggca ctcggttatg
     4561 aattaccgcg atgctacaat gtggcgcgaa gaatgttaac cctctggagc gttttgtgtc
     4621 gtcgttgccc gttgctgccg tcttacctga attacttacc gctctcgatt gtgcgccgca
     4681 ggtattatta agtgcgccga ccggggccgg gaaatcaacc tggctgccgc tgcaactgct
     4741 ggcgcatccc ggcattaacg ggaaaattat cctgctggag ccgcgtcgtc tggcggcgcg
     4801 taacgtcgcg caacggctgg cggagctgct taacgaaaag ccaggcgata ccgttggcta
     4861 ccggatgcgt gcgcaaaact gcgtcgggct gaatacccgc ctggaagtgg ttaccgaagg
     4921 cgtgctgacg cggatgatcc agcgtgaccc ggaactgagc ggtgttggat tggtgatcct
     4981 tgatgagttt catgagcgca gtttgcaggc ggatttggcg ttggcgctgt tactcgatgt
     5041 gcaacaaggt ctgcgtgatg accttaaact gctgattatg tcggctacgc tggacaatga
     5101 ccgcttgcag caaatgctgc cggaagcgcc cgtcgtcatc tcagaagggc gctcgtttcc
     5161 ggttgaacgc cgttatttac cgctgcccgc gcatcagcgt tttgacgaag ccgttgcggt
     5221 agccaccgcc gaaatggtgc gtcaggaaag tggatcatta ctgttatttt tacctggcgt
     5281 cggagaaatt cagcgcgtgc aggaacaact ggcttcgcgc atcggcagtg atgtggtgct
     5341 ctgcccgctg tatggcgtgt tgtcactgaa cgatcagcga aaagcgatcc tcccggcacc
     5401 gcaagggatg cgcaaagtgg tgctggcgac caatattgct gaaaccagtt taaccatcga
     5461 aggtattcgt ctggtggtgg attgtgccca ggagcgtgtg gcgcgctttg atccgcgcac
     5521 ggggcttacg cggttgatta ctcaacgcgt tagtcaggca tccatgacgc agcgtgccgg
     5581 gcgcgccggg cgtctggagc cgggtatcag cctgcattta atcgccaaag aacaagcaga
     5641 acgcgccgcg gcgcaaagtg agccgaagat cttacaaagc gatctttccg gtttgctgat
     5701 ggaattactg caatggggat gcagcgatcc ggcgcagatg agctggctgg atcaaccgcc
     5761 aacggtgaat ctactggccg cgaaacgcct gttacaaatg ctgggggcgc tggagggtga
     5821 acggcttagt gcgcaagggc aaaaaatggc agcgctgggt aacgatccgc gtttagcggc
     5881 aatgctggtt aacgcaaaga gcgatgacga agctgctacc gcggcaaaaa ttgccgccat
     5941 tctcgaagag ccgccacgga tgggcaatag tgacctgggc gtggcgtttt cgcgcaatca
     6001 accagcctgg cagcaacgta gtcagcaact gttaaaacgc ttaaacgtac gcggcggtga
     6061 ggcagacagt tcgcttatcg cgccgctact tgccggggcg tttgccgatc gcattgctca
     6121 tcgccgtggg caagatggac gctatcaact ggcaaacagc atgggagcga tgctcgatgc
     6181 cgacgacgcg ctaagccgcc acgaatggtt gatcgcaccg ttattattgc agggcagcgc
     6241 ctcgccggat gcgcggattt tactggcgct accggtcgat attgatgaat tagtacaacg
     6301 ctgtccgcag ctggtgcagc aatctgacac cgtggaatgg gatgacgcgc aaggtacgct
     6361 gaaagcctgg cgtcggctac aaatcggtca gttgacggtg aaagtgcagc cgctggcgaa
     6421 accgtcagaa gacgagttgc atcaggcgat gcttaatggc atccgtgata aaggtttaag
     6481 cgtgctcaac tggacggcgg aagcggaaca gctacgcttg cgtttgttat gcgccgcaaa
     6541 gtggttgccg gaatatgact ggccagcggt tgatgatgaa agtttgttgg cgacgctgga
     6601 aacgtggctg ctgccacata tgaccggcgt acattcacta cgcggcctga aatcactcga
     6661 catttatcag gcactacgcg gattacttga ttggggaatg cagcaacgtc tggatagtga
     6721 attgcctgcg cattacactg tgccgacggg aagccggatc gccattcgtt atcatgaaga
     6781 taacccgccc gcgctggcgg tgagaatgca ggagatgttt ggtgaggcca ctaatccgac
     6841 gatcgcccag gggcgcgtgc cgctggtgct gaagttgctt tcccctgccc aaaggccgct
     6901 gcaaatcacg cgtgatttgg gggccttctg gaaaggggcg tatcgtgagg tgcaaaagga
     6961 gatgaaaggg cgttatccca aacacgtctg gccggacgac ccggcaaaca cggcaccgac
     7021 gcgacggacg aaaaagtatt cgtaaggttg gttttctccc tctccctgtg ggagagggcc
     7081 ggggtgaggg catcagcgcg cacgttcacc ctcaccctaa ccctctccct caagggagag
     7141 gggaccgatc gagcgcaaat tttgagagat atcttcttct gtcttgtaac agaagaacag
     7201 aaaatcgggc ttttgcgcct gaatattgcg gagaaaaagc atggccggga atgaccgcga
     7261 gccaattgga cgcaaaggga aaccgacgcg tccggtcaaa caaaaggtaa gccgtcgtcg
     7321 ttacgaagat gacgatgatt acgacgatta tgatgactat gaggatgaag aaccgatgcc
     7381 gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt ggcaaacgcg gctggctatg
     7441 gctactgcta aaactggcta tcgtttttgc cgtgctgatc gccatttacg gcgtttatct
     7501 cgatcaaaaa attcgtagcc gtattgatgg caaggtctgg caactgcccg cggcagttta
     7561 tggccgaatg gtcaaccttg aaccagatat gaccatcagc aagaacgaaa tggtgaagct
     7621 gctggaggca acacagtatc gtcaggtgtc gaaaatgacc cgtcctggcg aatttaccgt
     7681 gcaggccaac agcattgaga tgatccgccg tccgtttgat ttcccggaca gtaaagaagg
     7741 gcaggtgcgt gcgcgtctga cctttgatgg tgatcatctg gcgaccatcg tcaatatgga
     7801 gaacaaccgt cagttcggtt tcttccgtct tgatccgcgt ctgatcacca tgatctcctc
     7861 gccaaacggt gagcagcgtt tgtttgtgcc gcgcagtggt ttccctgatt tgctggtgga
     7921 tactttgctg gcgacagaag accgtcattt ttacgagcat gatggaatca gtctctactc
     7981 cattgggcgt gcggtgctgg caaacctgac cgcaggtcgc acggtgcagg gtgcgagtac
     8041 gctgacgcaa cagctggtga aaaacctgtt cctctccagc gagcgttctt actggcgtaa
     8101 agcgaacgaa gcttacatgg cgctgatcat ggacgcgcgt tacagcaaag accgtattct
     8161 tgagctgtat atgaacgagg tgtatctcgg tcagagcggc gacaacgaaa tccgcggctt
     8221 cccgctggca agcttgtatt actttggtcg cccggtagaa gagctaagcc tcgaccagca
     8281 ggcgctgtta gtcggtatgg tgaaaggggc gtccatctac aacccgtggc gtaacccgaa
     8341 actggcgctg gagcgacgta atctggtgct gcgtctgctg caacagcaac agattattga
     8401 tcaagaactc tatgacatgt tgagtgcccg tccgctgggg gttcagccgc gtggtggggt
     8461 gatctctcct cagccagcct ttatgcagct ggtgcgtcag gagttgcagg caaaactggg
     8521 cgataaggta aaagatctct ccggcgtgaa gatcttcact acctttgact cggtggccca
     8581 ggacgcggca gaaaaagccg ccgtggaagg cattccggca ctgaagaaac agcgtaagtt
     8641 gagcgatctt gaaactgcga ttgtggtcgt cgaccgcttt agtggtgaag ttcgtgcgat
     8701 ggtcggaggt tctgagccgc agtttgcggg ctacaaccgt gcgatgcagg cgcgtcgttc
     8761 gattggttcc cttgcaaaac cagcgactta tctgacggct ttaagccagc cgaaaatcta
     8821 tcgcctgaac acgtggattg ccgatgcgcc aatcgcgctg cgtcagccga atggccaggt
     8881 ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc ggcagagtga tgctggtgga
     8941 tgcgttgaca cgttcgatga acgtgccgac ggtaaatctg gggatggcgc tggggctgcc
     9001 tgcggttacg gagacctgga tcaaactggg cgtaccgaaa gatcagttgc atccggttcc
     9061 ggcaatgctg ctgggggcgt tgaacttaac gccaatcgaa gtggcgcagg cattccagac
     9121 catcgccagc ggtggtaacc gtgcaccgct ttctgcactg cgttcggtaa tcgcggaaga
     9181 tggtaaggtg ctgtatcaga gcttcccgca ggcagaacgc gctgttccgg cgcaggcggc
     9241 gtatctgaca ctatggacca tgcagcaggt ggtacaacgt ggtacgggtc gtcagcttgg
     9301 ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg actaccaaca ataacgtaga
     9361 tacctggttt gcaggcattg acggcagcac ggtgaccatc acctgggtag gccgtgataa
     9421 caaccagccg accaaactgt atggagccag cggggcaatg tcgatttatc agcgttatct
     9481 ggctaaccag acgccaacgc cgctgaatct tgttccgcca gaagatattg cagatatggg
     9541 cgtggactac gacggcaact ttgtttgcag cggtggcatg cgtgtcttgc cggtctggac
     9601 cagcgatccg caatcgctgt gccagcagag cgagatgcag cagcagccgt caggcaatcc
     9661 gtttgatcag tcttctcagc cgcagcaaca gccgcaacag caacctgctc agcaagagca
     9721 gaaagacagc gacggtgtag ccggttggat caaggatatg tttggtagta attaacatct
     9781 aagcgtgaaa taccggatgg cgagttgcca tccggtaaaa taacatccca tctaagatat
     9841 taaccctttc ttttcatctg gttgtttatt aacccttcag gaacgctcag attgcgtacc
     9901 gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt tataataatc attctcgttt
     9961 acgttattat tcactttaca tcagagatat accaatggcg cgttccaaaa ctgctcagcc
    10021 aaaacaatca ctgcgtaaaa tcgcagttgt agtagccaca gcggttagcg gcatgtctgt
    10081 ttatgcacag gcagcggttg aaccgaaaga agacactatc accgttaccg ctgcacctgc
    10141 gccgcaagaa agcgcatggg ggcctgctgc aactattgcg gcgcggcagt cagctaccgg
    10201 cactaaaacc gatacgccga ttcaaaaagt gccacagtct atttctgttg tgaccgccga
    10261 agagatggcg ctgcatcagc cgaagtcggt aaaagaagcg cttagctaca cgccgggtgt
    10321 ctctgttggt acgcgtggcg catccaacac ctatgaccac ctgatcattc gcggttttgc
    10381 ggcagaaggc caaagccaaa ataactatct gaatggcctg aagttgcagg gcaacttcta
    10441 taacgatgcg gtcattgacc cgtatatgct ggaacgcgct gaaattatgc gtggcccggt
    10501 ttccgtgctt tacggtaaaa gcagtcctgg cggtctgttg aatatggtca gcaagcgtcc
    10561 gaccaccgaa ccgctgaaag aagttcagtt taaagccggt actgacagcc tgttccagac
    10621 tggttttgac tttagcgatg cgttggatga tgacggtgtt tactcttatc gcctgaccgg
    10681 tcttgcgcgt tctgccaatg cccagcagaa agggtcagaa gagcagcgtt atgctattgc
    10741 accggcgttc acctggcgtc cggatgataa aaccaatttc accttccttt cttacttcca
    10801 gaacgagccg gaaaccggtt attacggctg gttgccgaaa gagggaaccg ttgagccgct
    10861 gccgaacggt aagcgtctgc cgacagactt taatgaaggg gcgaagaaca acacctattc
    10921 tcgtaatgag aagatggtcg gctacagctt cgatcacgaa tttaacgaca cctttactgt
    10981 gcgtcagaac ctgcgctttg ctgaaaacaa aacctcgcaa aacagcgttt atggttacgg
    11041 cgtctgctcc gatccggcga atgcttacag caaacagtgt gcggcattag cgccagcgga
    11101 taaaggccat tatctggcac gtaaatacgt cgttgatgat gagaagctgc aaaacttctc
    11161 cgttgatacc cagttgcaga gcaagtttgc cactggcgat atcgaccaca ccctgctgac
    11221 cggtgtcgac tttatgcgta tgcgtaatga catcaacgcc tggtttggtt acgacgactc
    11281 tgtgccactg ttcaatctgt acaatccggt gaatcccgat ttcgacttca atgccaaaga
    11341 tccggcaaac tccggccctt accgcattct gaataaacag aaacaaacgg gcgtttatgt
    11401 tcaggatcag gcgcagtggg ataaagtgct ggtcacccta ggcggtcgtt atgactgggc
    11461 agatcaagaa tctcttaacc gcgttgctgg gacgaccgat aaacgtgatg acaaacagtt
    11521 tacctggcgt ggtggtgtta actacctgtt tgataatggt gtaacacctt acttcagcta
    11581 tagcgaatcg tttgaacctt cttcgcaagt tgggaaggat ggtaatattt tcgcaccgtc
    11641 taaaggtaag cagtatgaag tcggcgtgaa atatgtaccg gaagatcgtc cgattgtagt
    11701 tactggtgcc gtgtataatc tcactaaaac caacaacctg atggcggacc ctgagggttc
    11761 cttcttctcg gttgaaggtg gcgagatccg cgcacgtggc gtagaaatcg aagcgaaagc
    11821 ggcgctgtcg gcgagtgtta acgtagtcgg ttcttatact tacaccgatg cggaatacac
    11881 caccgatact acctataaag gcaatacgcc tgcacaggtg ccaaaacaca tggcttcgct
    11941 gtgggctgac tataccttct ttgacggtcc gctttcaggt ctgacgctgg gcaccggtgg
    12001 tcgttatact ggctccagct atggtgatcc ggctaactcc tttaaagtgg gaagttatac
    12061 ggtcgtggat gcgttagtgc gttatgatct ggcgcgagtc ggcatggcgg gctccaacgt
    12121 ggcgctgcat gttaacaacc tgttcgatcg tgaatacgtc gccagctgct ttaacactta
    12181 tggctgcttc tggggcgcag aacgtcaggt cgttgcaacc gcaacctttc gtttctaatt
    12241 tctcttttgg ggcacggatt tccgtgccca tttcacaagt tggctgttat gcaggaatac
    12301 acgaatcatt ccgataccac ttttgcactg cgtaatatct cctttcgtgt gcccgggcgc
    12361 acgcttttgc atccgctgtc gttaaccttt cctgccggga aagtgaccgg tctgattggt
    12421 cacaacggtt ctggtaaatc cactctgctc aaaatgcttg gtcgtcatca gcctccgtca
    12481 gaaggggaga ttcttcttga tgcccagccg ctggaaagct ggagcagcaa agcgtttgcc
    12541 cgcaaagtgg cttatttgcc gcagcagctt cctccggcag aagggatgac cgtgcgtgaa
    12601 ctggtggcga ttggtcgtta cccgtggcat ggcgcgctgg ggcgctttgg tgcggcagat
    12661 cgcgaaaagg tcgaggaagc tatctcgctg gttggcttaa aaccgctggc gcatcggctg
    12721 gtcgatagtc tctctggcgg cgaacgtcag cgggcgtgga tcgccatgct ggtggcgcag
    12781 gatagccgtt gtctgttgct cgacgaaccg acttcagcgc tggatatcgc ccaccaggtt
    12841 gatgtgctgg cgctggtgca ccgtttaagt caggaacgtg gcctgacggt cattgccgtg
    12901 ttgcacgata tcaatatggc ggcacgctac tgtgattatc tggtcgccct gcgcagtggt
    12961 gaaatgattg ctcagggaac gcctgcggaa attatgcgcg gcgaaaccct cgaaatgatt
    13021 tatggcatcc cgatgggtat tttgccgcat ccggcgggtg ctgcacctgt gagttttgtt
    13081 tattgatgag cggcttacct cttatttcgc gccgtcgact gttaacggcg atggcgcttt
    13141 ctccgttgtt atggcagatg aataccgccc acgcggcggc tattgacccc aatcgtattg
    13201 tggcgctgga gtggttgccg gtggaattac tgctggcgct cggcatcgtg ccttacggcg
    13261 tggcggatac catcaactat cgcctgtggg tcagcgaacc accattgccg gactcagtga
    13321 ttgacgtcgg tttgcgtaca gaacctaacc ttgaactgct gaccgaaatg aaaccgtcgt
    13381 ttatggtctg gtcggcagga tatggccctt catcagaaat gctggcacgt attgcgccgg
    13441 gtcgcggatt taacttcagt gacggcaaac atccgttggc gatggcgcgt aaatcgctga
    13501 cggaaatggc agatttactt aacctgcaaa gcgcagcgga aacgcattta gcgcaatacg
    13561 aagactttat ccgcagcatg aaaccccgct ttgtgaagcg tggtgcacgc ccgttactgc
    13621 tgacgacgct tatcgatccg cgccatatgc tggtcttcgg tccaaacagc ttgttccagg
    13681 aaattctcga tgagtacggc atcccaaatg cctggcaagg ggaaaccaac ttctggggca
    13741 gtaccgccgt cagtatcgat cgtctggcag cgtataaaga cgttgatgtg ctctgttttg
    13801 atcacgacaa cagcaaagac atggatgcgc taatggcaac cccgctgtgg caggccatgc
    13861 cgtttgtccg tacagggcgc tttcagcgcg tacccgcagt ctggttttat ggtgcaacgc
    13921 tttcggcaat gcactttgtg cgtgttctgg ataacgccat cggaggtaaa gcgtgagtaa
    13981 acgaattgcg cttttcccgg cgttattgct ggcgctgtta gtgattgtcg ctacggcgct
    14041 cacctggatg aacttctcac aggcgctgcc gcgcagtcag tgggcgcagg ccgcctggtc
    14101 gccggatatt gacgtcatcg agcagatgat ttttcactac agcttgttgc cgcgtctggc
    14161 gatttcgctg ctggtgggcg cgggcctggg gctggtgggc gtgctgtttc agcaagtact
    14221 gcgtaacccg ctggcggaac cgacgacgtt gggcgttgca acaggcgcgc aactggggat
    14281 taccgtcacc acgctctggg cgatccctag cgcgatggcg agccagtttg ctgcgctggc
    14341 aggggcttgc gttgttggct taattgtctt tggcgtcgcg tgggggaaac ggctgtcgcc
    14401 ggtaacgctg attttcgcgg ggttggtagt gagcctttat tgcggcgcaa tcaatcagtt
    14461 actggttatc ttccatcatg accagttgca aagcatgttt ctgtggagca ctggaacgct
    14521 gacgcaaacc gactggggcg gtgttgagcg tttatggccg cagttgctgg gcggtgtgat
    14581 gctgacgttg ctgctacttc gtccgttaac cctgatgggg cttgatgatg gcgtggcgcg
    14641 caatctcggg ctggccttgt cgcttgcgcg tctggcggcg ctgtcgctgg cgattgtcat
    14701 cagtgcgctg ctggtgaacg ctgtggggat tatcggcttt atcgggttgt tcgcgccgct
    14761 gctggcaaaa atgctggggg cgcggcgtct gctgccacga ctgatgctgg cgtcgttgat
    14821 tggtgcgctg atcctctggc tttccgatca aatcatcctc tggctgactc gcgtgtggat
    14881 ggaagtgtcc accggttcgg tcactgcgtt gatcggtgcg ccgctgctac tgtggctgct
    14941 gccgcgttta cgcagcatta gcgcgccgga tatgaaggtt aacgatcgtg tcgcgactga
    15001 acgccaacat gtgctggcgt ttgccctcgc gggcggcgtg ctgttgttga tggctgtggt
    15061 ggtggcgctg tcgtttggtc gtgatgcgca cggctggacg tgggcgagcg gggcgttgct
    15121 cgaggattta atgccctggc gctggccgcg aattatggcg gcgctgtttg cgggcgtcat
    15181 gctggcggta gcgggctgta ttattcagcg actgaccggc aacccgatgg caagcccgga
    15241 agtgctgggg attagctccg gcgcggcgtt tggtgtggtg ttaatgctgt ttctggtacc
    15301 gggtaatgcc tttggctggt tgttacccgc aggcagtctc ggggcggcgg tgacgctgtt
    15361 gatcattatg atcgccgccg gacgcggtgg attttcccca caccgcatgt tactggcggg
    15421 gatggcgtta agtaccgcgt tcaccatgct tttgatgatg ttgcaggcaa gtggtgaccc
    15481 gcgaatggcg caagtgctga cctggatttc cggttcgacc tacaacgcga ccgatgctca
    15541 ggtctggcgc accggaattg tgatggtgat tttgctggcg attactccgc tgtgccgccg
    15601 ctggctgacc attttaccgc tgggtggtga taccgcgcgg gcggtaggga tggcgctgac
    15661 gccgacgcga attgcgctgc tgctgttagc ggcttgcctg acggcgaccg cgacgatgac
    15721 tattggaccg ttgagttttg ttggtttaat ggcaccgcat attgcgcgga tgatgggctt
    15781 tcgacggacg atgccacaca tcgtaatttc ggcgctggtg ggtggtttac tgctggtgtt
    15841 cgctgactgg tgtgggcgga tggtgctgtt tccattccag atcccggcgg ggctgctgtc
    15901 gacctttatc ggtgcgccat attttatcta tttgctgaga aagcagagcc gttaatagaa
    15961 tgcctcgccc ggtgggcgag gcaaaattat cacaacttcg cgaacacccg acgtgcagca
    16021 tcgatggtgt tattgatatc ttccatgctg tgcgccacag acataaagcc tgcttcaaac
    16081 gctgacggtg ccaggtaaac accttcatcc agcatcatat ggaagaaacg cttaaaacgc
    16141 tcgacgtcac aggccatcac atcctgatag cacgtcacgg actcggcgtc ggtaaagaag
    16201 ataccgaaca taccaccgac gtggttaacg accagcggaa ttcccgcttc ttctgccgct
    16261 tccagcagac cttctgccag acgtgttgtc agctcatcca gcgtttcgtg aatgcccggc
    16321 tgcgcgactt cattcagaca ggcgaaaccc gctgccatcg caatcgggtt accggaaagc
    16381 gtacccgcct gatagaccgg acctgtcggg gccagcgcat ccattacatc acgacgacca
    16441 ccgaatgcgc ctaccggcat tccaccgccg atgatttttc cgaggcaggt taaatctggc
    16501 actacggcga aataatcctg tgcgccagct agcgctacgc ggaaaccggt catcacttca
    16561 tcgatgatca gcaacgcgcc aaattcgtcg cacagcgcac gcagacctgg caggaactct
    16621 ggcagcggcg gaacacagtt catattgcct gccaccggct cgacgataat acaggcaatc
    16681 tcttgcgggt attgctcaaa cgcggcgcgt acagaggcca gatcgttata agtacaggtt
    16741 aaggtatgtt tggcgaaatc tgccggaacg cccggcgagt ttggctggcc taacgtgagt
    16801 gcgccagaac cggctttcac cagcaggcag tcagcgtgac cgtggtaaca accttcaaat
    16861 ttaatgattt tgtcgcgacc ggtaaaacca cgggccaggc ggatggcgct catcgtcgcc
    16921 tcggtgccgg agttcaccat gcgcaccata tccatggtcg ggaccagttc ggtcaccagt
    16981 tgcgccattt tcacttccat ttcggtcggt gcaccaaagc ttaaaccacg ctcggcggct
    17041 tcaatcacgg cattgcggat cgccggatgg ttatggccca gcaccatcgg cccccaggaa
    17101 ccgacataat cgatataggc tttgccatca acatcgtaca gataagcgcc gtccgctttt
    17161 tcgataaaca gtggagtgcc gcccacgcca gtaaaggcgc gaacagggga gttcacaccg
    17221 ccagggatca gctcgcgcgc tgcgctgtaa agattttcag acttactcat ggagggttcc
    17281 tgattcgtag aaaaagtgaa tggctgctat tccatgttat tcataacaag ttaaatactc
    17341 gtcaaacatc aggctgcttg tactggtgca atcctgattt cttagagtat aaaagttttg
    17401 tgcatttgaa acattacgct ttgcaaagga ttttcatgga acgtgcgagt aaaatgccgt
    17461 catcttattt gtatgaccaa taagtgatca ttggatgaaa actgatactc cctctttaga
    17521 aacaccgcag gccgcgcgcc tgcgacgcag acaactgatt cgccaacttc ttgagcgcga
    17581 taaaaccccg ttagccattt tgtttatggc ggcagtcgtc ggcacgcttg tcgggctggc
    17641 tgcggttgct tttgacaaag gtgtcgcctg gttgcagaac caacgtatgg gggcgctggt
    17701 acatactgct gataattatc cgctactgtt aaccgtcgct tttctctgtt cggcggtgct
    17761 ggcgatgttc ggctactttt tggtgcgcaa atacgcgccc gaagcagggg gatcggggat
    17821 cccggaaatt gaaggggcgc tggaagatca acgtcctgtt cgctggtggc gtgtattgcc
    17881 ggtgaaattc tttggcgggc tggggacact cggcggaggc atggggctag ggcgcgaagg
    17941 accaaccgtg cagatcggcg gcaatatcag tcgcatggtg cttgatattt tccgcctgaa
    18001 aggtgatgaa gctcgccata cgctgctggc aaccggtgct gctgcggggc tggctgcggc
    18061 ctttaacgcg ccgctggcgg gtattttgtt tattatcgaa gagatgcgtc cgcagtttcg
    18121 ctatacgtta atttcgatta aagcggtatt tattggcgtc attatgtcga ccattatgta
    18181 ccggattttt aatcatgaag ttgcgttgat tgacgtcggt aaactttctg atgcgccgct
    18241 taatacgctg tggctttatc tgatcctcgg tattattttt ggcattttcg gccctatttt
    18301 taataaatgg gtgctgggga tgcaggattt gctgcaccgt gtgcacggcg gcaatattac
    18361 caaatgggta ctgatggggg gggcgattgg cggtctgtgt ggattgctgg ggtttgtggc
    18421 accagcaacg tcgggcggcg gttttaacct gattcctatc gctaccgcgg ggaatttcag
    18481 catgggaatg ctggtgttta tcttcgtcgc tcgggtcatt accaccttac tctgcttctc
    18541 ttccggcgcg ccgggcggta tttttgcccc gatgctggcg ctgggtactg tgctgggaac
    18601 cgctttcgga atggttgccg ttgagctgtt tccgcaatat cacctggagg cggggacgtt
    18661 tgctattgcc ggaatggggg cattactggc ggcatctatt cgcgcgccgt taacggggat
    18721 cattctggtt ctggagatga ccgataacta ccagctcatt ttgccaatga ttattaccgg
    18781 tcttggcgca acactattag cgcaatttac cggcgggaaa cctctatact cggcgattct
    18841 tgcgcgcacg ctggcaaaac aggaagctga gcaactggcg cgaagcaagg ccgcatcagc
    18901 cagcgagaat acttgaacga aataccaggg tattagataa tggcgattat tattgggtta
    18961 gaatttgccc gattgccgat gtcgtttgga gcaaaatatg agtgatgacg tagcactgcc
    19021 gctggagttt accgacgcag cagccaacaa agttaaaagc ctgatcgctg acgaagataa
    19081 cccgaatctg aaattacgcg tgtatatcac cggtggcggt tgcagcggct tccagtatgg
    19141 tttcaccttt gatgatcagg tgaacgaagg cgatatgacc atcgaaaaac agggcgttgg
    19201 cctggtggtt gatccgatga gcctgcaata tctggtcggc ggttccgttg actacaccga
    19261 aggactggaa ggttctcgtt tcatcgtgac caacccgaac gcgaaaagca cctgcggttg
    19321 cggttcttcc tttagcatct aatctgttgt tcatgtgccg gataaactat ccggcacatc
    19381 ttctctcttc aacgcccatt ctcatccagc gcaaacgtcg gtagcttaag atgccaacga
    19441 atagccgcca gccgaatcaa tagcgtcacg accatgccca tcatactggc tgtttccagt
    19501 ggtacggaaa atgtgtaata agccgtagcg tggacaatac cgccgataat acaggcagtt
    19561 gcgtagattt ctgtacgtaa aatcatgggg atttcgcgga ccagtacatc acgaatgatc
    19621 ccgccgccaa cgccagtaat gacgcccata caaaccgcga ttaacggacc ggcttccgca
    19681 ttaaaggctt tattcacgct aatgccgaca aacaccgcca gaccaacggc gtccagcacc
    19741 ggcaacatcc attttggtaa gcgtcttggc tggcgcacca gcacgatggt cagcatgctg
    19801 gtgaccatgg caacgaccag atcggtggga tctttcaccc aaaataccgg gccgtgatcc
    19861 agcgccatgt cgcgaattgt tccgtcgcct actgcggtaa ccacgcccag taccagaaca
    19921 ccaaaagggt ccatacgcaa ttttccggct aacaaaacgc cgtagatggc aaataccgct
    19981 gtgccgacta tatccagcca atagacgagc attgttcaat ccccactgag cacctgaaaa
    20041 ggtcaggcac taatctacct gtgaaagtgc attacagagc tgttgtgctg cgaggataat
    20101 acgtgggctt gcacgttcaa accagtcact cgtgagagga ataacgggaa ttttgagctg
    20161 ttcaccccag tattgtttga ttttaggaat ttggtccggt ccgcctgtaa tgacaatagc
    20221 ctgtggcgag cgtgctaaca cctgttcgcg gctaacttgc ggccagggaa cccggctgtc
    20281 tttaaagatg ttttctccgc cacaaacttc gagtacctgg ttctgaatcg actcttttcc
    20341 actggtaaat ggcggattaa tgccgaattg cagaaaaaca cgttttttag gtttatcagc
    20401 atattgcgct ttcaattgca cgtactgatc cagcagggat tgcgcggctt gttcggcctt
    20461 gtctggttgc ggactccagg gggccagttg acgtaacgca ttggcaattt gttcaatgct
    20521 tgtcgcatcg acccacatca cttttattcc cagcgaagcc agctggtcaa cctgccgctc
    20581 ggcattacct ccacgccagg caatcaccag atcgggtttc agcgcgacaa tgcgttccag
    20641 attcatcccc tgccaggtgg aaacctgctc aatcttttgc gcttgtggag gatagtcgga
    20701 atagctgctg accccaaccg gcgtgatccc ggcggcaaag gcaagttcag tgttggcggg
    20761 agaaagcgtg atgacgcgcg gcgcggcgtt gagccacagt ggcgcgagaa aagacagggc
    20821 gaccagcgcc ctgaacagtg atttagccat gtgccagttt ctgcaccagt gactcaacca
    20881 tcaggctgga ctgtttagcg gcaacagcca ggaactcatc gaagctaaga tgagactgtt
    20941 gatcggccac gtcggagatg gcgcgtacga caacaaacgg gacgttgaaa ttgtggcaga
    21001 catgggcgat tgccgtcgct tccatctcta cagcaatggc ctgtgggaag ttgtggcgga
    21061 ttttcgccag accaacagaa ccgttgatga aagcgtcgcc gctaacaatc aggccacgta
    21121 cagcgttaag attcagttcg gcaatgcagg cctcagcggc agcgatcagt ttatcgtcag
    21181 ctttaaagcc tgccggacag cctggtaact gaccgtattc ataaccaaat gccgtgacat
    21241 ccgcgtcgtg ataacgcgct tcgtccgaga caacgatatc gcccactttc agcgttggtg
    21301 ccaggccacc ggcagaaccg gtgttaataa tcacatctgg cttgcagtgt tccaacagca
    21361 gagtggcacc cagcgccgca gcgactttac cgatgcccga tttcagaagc gcaacctcgg
    21421 ttccattcag ttggccggta tagatttcgc aaccgccgag actgatagtt tgacggtttt
    21481 cgattttgtc acgcagcagc gtaacttctt cttccattgc accaatgatg ccgattttca
    21541 tagatttact cgcgataagc ccgatttgaa ggcatagttt accatgcgct tacggggaag
    21601 cgtatttctc acgcgggaga ggacatggca cagattgatt tccgaaaaaa aataaactgg
    21661 catcgtcgtt accgttcacc gcagggcgtt aaaaccgaac atgagatcct gcggatcttc
    21721 gagagcgatc gcgggcgtat catcaactct ccggcaattc gtcgtctgca acaaaagacc
    21781 caggtttttc cactggagcg caatgccgcc gtgcgcacgc gtcttaccca ctcgatggaa
    21841 gtccagcagg tgggacgcta catcgccaaa gaaattttaa gccgtctgaa agagcttaaa
    21901 ttgctggaag catacggcct ggatgaactg accggcccct ttgaaagcat tgttgagatg
    21961 tcatgcctga tgcacgatat cggcaatccg ccgtttggtc attttggcga agcagcgata
    22021 aatgactggt ttcgccaacg tttgcacccg gaagatgccg aaagccagcc tctgactgac
    22081 gatcgctgca gcgtggcggc actacgttta cgggacgggg aagaaccgct taacgagctg
    22141 cggcgcaaga ttcgtcagga cttatgtcat tttgagggga atgcacaagg cattcgtctg
    22201 gtgcatacat tgatgcggat gaatctcact tgggcacagg ttggcggtat tttaaaatat
    22261 acccgtccgg cgtggtggcg tggcgaaacg cctgagacac atcactattt aatgaaaaag
    22321 ccgggttatt atctttctga agaagcctat attgcccgct tgcgtaaaga acttaatttg
    22381 gcgctttaca gtcgttttcc attaacgtgg attatggaag ctgccgacga catctcctat
    22441 tgtgtggcag accttgaaga tgcggtagag aaaagaatat ttaccgttga gcagctttat
    22501 catcatttgc acgaagcgtg gggccagcat gagaaaggtt cgctcttttc gctggtggtt
    22561 gaaaatgcct gggaaaaatc acgctcaaat agtttaagcc gcagtacgga agatcagttt
    22621 tttatgtatt tacgggtaaa caccctaaat aaactggtac cctacgcggc acaacgattt
    22681 attgataatc tgcctgcgat tttcgccgga acgtttaatc atgcattatt ggaagatgcc
    22741 agcgaatgca gcgatcttct taagctatat aaaaatgtcg ctgtaaaaca tgtgtttagc
    22801 catccggatg tcgagcagct tgaattgcag ggctatcggg tcattagcgg attattagag
    22861 atttatcgtc ctttattaag cctgtcgtta tcagacttta ctgaactggt agaaaaagaa
    22921 cgggtgaaac gtttccctat tgagtcgcgc ttattccaca aactctcgac gcgccatcgg
    22981 ctggcctatg tcgaggctgt cagtaaatta ccgtcagatt ctcctgagtt tccgctatgg
    23041 gaatattatt accgttgccg cctgctgcag gattatatca gcggtatgac cgacctctat
    23101 gcgtgggatg aataccgacg tctgatggcc gtagaacaat aaccagtctt ttgtaaagac
    23161 gaacaataaa tttttacctt ttgcagaaac tttagttcgg aacttcgggc tataaaacga
    23221 atctgaagaa cacagcaatt ttgcgttatc tgttaatcga gactgaaata catgaaaaaa
    23281 aacacattag cactgagtgc actggctctg agtttaggtt tggcgttatc tccgctctct
    23341 gcaacggcgg ctgagacttc ttcagcaacg acagcccagc agatgccaag ccttgcaccg
    23401 atgctcgaaa aggtgatgcc ttcagtggtc agcattaacg tagaaggtag cacaaccgtt
    23461 aatacgccgc gtatgccgcg taatttccag cagttcttcg gtgatgattc tccgttctgc
    23521 caggaaggtt ctccgttcca gagctctccg ttctgccagg ggggccaggg cggtaatggc
    23581 ggcggccagc aacagaaatt catggcgctg ggttccggcg tcatcattga tgccgataaa
    23641 ggctatgtcg tcaccaacaa ccacgttgtt gataacgcga cggtgattaa agtccagctg
    23701 agcgatggtc gtaagttcga cgcgaagatg gttggcaaag atccgcgctc tgatatcgcg
    23761 ctgatccaaa tccagaaccc gaaaaaactg accgcaatta agatggcgga ttctgatgca
    23821 ctgcgcgtgg gtgattacac cgtagcgatt ggtaacccgt ttggtctggg cgagacggta
    23881 acttccggga ttgtctctgc gctggggcgt agcggcctga atgctgaaaa ctacgaaaac
    23941 ttcatccaga ccgatgcagc gatcaaccgt ggtaactccg gtggtgcgct ggttaacctg
    24001 aacggcgaac tgatcggtat caacaccgcg atcctcgcac cggacggcgg caacatcggt
    24061 atcggttttg ctatcccgag taacatggtg aaaaacctga cctcgcagat ggtggaatac
    24121 ggccaggtga aacgcggtga gctgggtatt atggggactg agctgaattc cgaactggcg
    24181 aaagcgatga aagttgacgc ccagcgcggt gctttcgtaa gccaggttct gcctaattcc
    24241 tccgctgcaa aagcgggcat taaagcgggt gatgtgatca cctcactgaa cggtaagccg
    24301 atcagcagct ttgccgcact gcgtgctcag gtgggtacta tgccggtggg cagcaaactg
    24361 accctgggct tactgcgcga cggtaagcag gttaacgtga acctggaact gcagcagagc
    24421 agccagaatc aggttgattc cagctccatc ttcaacggca ttgaaggcgc tgagatgagc
    24481 aacaaaggca aagatcaggg cgtggtagtg aacaacgtga agacgggcac tccggctgcg
    24541 cagatcggcc tgaagaaagg tgatgtgatt attggtgcga accagcaggc agtgaaaaac
    24601 atcgctgaac tgcgtaaagt tctcgacagc aaaccgtctg tgctggcact gaacattcag
    24661 cgcggcgaca gcaccatcta cctgttaatg cagtaatctc cctcaacccc ttcctgaata
    24721 cgggaagggg ttctccttac aatctgtgaa cttcgccaca actccataca tcttcatcat
    24781 cctttgggca tttgcacaat gccgtacgtt acgtacttcc ttatgctaag ccgtgcataa
    24841 cggaggactt atggctggct ggcatcttga taccaaaatg gcgcaggata tcgtggcacg
    24901 taccatgcgc atcatcgata ccaatatcaa cgtaatggat gcccgcgggc gaattatcgg
    24961 cagcggcgat cgtgagcgta ttggtgaatt gcacgaaggt gcattgctgg tactttcaca
    25021 gggacgagtc gtcgatatcg atgacgcggt agcacgtcat ctgcacggtg tgcggcaggg
    25081 gattaatcta ccgttacggc tggaaggtga aattgtcggc gtaattggcc tgacaggtga
    25141 accagagaat ctgcgtaaat atggcgaact ggtctgcatg acggctgaaa tgatgctgga
    25201 acagtcgcgg ttgatgcact tgttggcgca ggatagccgt ttgcgggaag aactggtgat
    25261 gaacctgatt caggcagagg agaatactcc cgcacttctg aatgggcgca acggctgggg
    25321 atcgatctca atcaaccgcg agtggtggct attgttgagg tcgacagcgg tcagcttggc
    25381 gtggacagcg caatggcgga gttacaacaa ctgcaaaacg cgctgactac gcccgagcgt
    25441 aataatctgg tggcgattgt ctcgctaacc gaaatggtgg tgttgaaacc ggcgttgaac
    25501 tcttttgggc gctgggatgc agaagatcat cgtaagcgag ttgaacaact gattacccgc
    25561 atgaaagagt acggccagct gcgttttcgc gtttcactgg gcaactattt taccggtcct
    25621 ggcagtattg cccgatccta tcgtacggcg aaaacgacga tggtggtggg taaacagcgg
    25681 atgccagaaa gtcgctgcta tttttatcag gatctgatgt tacctgtgtt actcgacagt
    25741 ttgcgtggcg actggcaggc caacgaactg gcgcgaccgc tggcgcggct gaaagcgatg
    25801 gacaataacg gcttgctgcg acgaacgctg gcggcgtggt ttcgccacaa tgtgcaaccg
    25861 ctggcaacgt caaaggcgtt gtttattcat cgtaataccc tggagtatcg gcttaatcgt
    25921 atatcggaac tgaccgggct tgatttgggc aattttgatg acaggttgct gctgtatgtg
    25981 gcgttacaac tggatgaaga gcggtagatt atgcgttaag gttgaggcgg cgctggctca
    26041 ttcccctcag cccgaagggg agaggggaat gcccgagtta accgtcaatt cgggattatt
    26101 tacgcgttag tttttccaga tcggcttcaa tctcgctgat cttattggtt acgactgact
    26161 ccaggtgacg taagtcgtcg aggatcttac gcttaagatc aacttcgctg cggtcacgct
    26221 gacagatttg atcaagctca tcaatgatat accgtagatt cgggctgatt tcctggactt
    26281 ctttataacc ctgacccaca ccatcagcga cgaccgtttt acgctgacgc ggatatttaa
    26341 acttaacgct cttggcgaaa aactcgcctt tgtctttctg gaaatagatt ttcagaatat
    26401 cgttgttggc ttcctgccgg aggctgtaac gatcaatttc ttcaggattg gtaataccca
    26461 gacttttcag attgtcgtac atagcgttac ctcaaaatga gtcagtaaat tgtgcttatt
    26521 ttagcatttg gcttggcccg cccggctcga ctgtgatcgc agaaagcctt tttcttccag
    26581 acttctatca tggcgtaaaa atcaaaaaat tacctgcttt attgtggcga tattccatgg
    26641 gatggattag cacatttatc ttcgttttgc gaattacatg acagtccgaa acaagatgaa
    26701 aaagtattta aaaaaggggg aggaagaatg agtaggacga attattctga gccagaaagc
    26761 atacgggcag catcatgctg cccgtatatg tactcttaat cgatggtacg cagcagttcg
    26821 ttaatgccga ctttgccgcg agttttcgcg tcaactttct taacgataac cgcacagtag
    26881 aggctgtatt tgccatcttt tgacggcaga ttacctgaaa caaccacaga ccccgccgga
    26941 acgcgaccgt agtggatttc gccggtttca cggtcgtaaa tacgggtgct ctgaccaatg
    27001 tatacgccca tagagatgac ggaaccttct tcgacaatca ccccttcaac cacttcagag
    27061 cgtgcgccaa tgaagcagtt atcttcaata atggtcgggt tagcctgcag cggctccagc
    27121 acgccgccga tgcccacgcc accggaaagg tggacgtttt taccaatctg cgcacaagaa
    27181 ccgacggtcg cccaggtatc aaccatggtg ccttcatcaa catatgcgcc gatgttaacg
    27241 taagacggca tcagcacggt gttacgggca ataaacgcac cctgacgtac cgccgctggt
    27301 ggcacaacgc ggaagccttc tttctggaaa cgtgcttcgt cgtagtcggc gaatttcatc
    27361 ggcactttgt cgaagtagcg gctttctgcc ccttcgatca cctgattatc attaatacgg
    27421 aaagagagca gcaccgcttt tttcaaccac tgatgcgtca cccactgacc gtcaattttt
    27481 tccgctacac gcagtgcgcc ggaatccagc agggcgatca cctgatttac cgcttcgcgg
    27541 gtaacggtgt ctgcattggc tggcgtgatc tcggcacggc gttcaaaagc ggtttcaata
    27601 atgttctgta actgctgcat tgttaaactc ttttcatatc agtaaacaca tcacccttta
    27661 tcgtttggat tgagggcctc tgtcaaccgc tgatgcactt cctgctgcaa ctcgttatta
    27721 agcgcacgcc ggtcagcggt ggcaattatg aataaatctt ctactcgctc gccaatggtt
    27781 gtaattcggg caccatgaag cgaaattccc agatcggcaa aaattttccc gactcgcgcc
    27841 agcagtccag gttggtcgag ggcgatcagt tcgaggaacg atttgcggtc ggtatgggtc
    27901 ggcaaaaacg ttacttcggt ttcaacagta aaatggcgta atttggcggg ttggcgacgg
    27961 ggctgcggtg gctgccagct actttgcgtc agcacctgct ccagaccaaa ccgaataacc
    28021 tcatggcgat ctgcggacag cgggctgcca tcgggttcca gcacgataaa ggtatccatc
    28081 gccataccgt cgcgagtggt gaaaatttgt gcgtcgtgaa cacttaaatt gcggcggtct
    28141 aattcggcac agacggcggc aaacagatag gggcggtccg ggctccagat aaaaatctcg
    28201 gtgcctccac gcgtagcctg cgggctaagc aataccagcg gtttgcttaa atcatgctgt
    28261 aataaatggc gggcatgcca ggccagttga tttgggctat ggcggacaaa atagttagca
    28321 cgacagcgtg accaaatttg gtgcagcgcc tcttcgtcga tgttatccat gcgcagtagt
    28381 gccagtgcct ggagttggtg atggcgaacc cgttcgcgca tatccggcgt gttttgcatt
    28441 ccgcgtcgta gctgcttttc ggtggcaaaa tagagttcgc gcaacagact ttgcttccag
    28501 ctattccaca gtgtttcgtt ggtggcgcaa atgtcagcca ctgtcaggca taccagatag
    28561 cgcagacgat tttccgtttg aacttcttcg gcaaactgct tgatgacttc agggtcctga
    28621 atatcgcggc gttgtgctgt caccgacatc agcaagtgct ggcgaaccag ccaggcaacc
    28681 agctgtgttt cgcgtgagtt cagcccgtgg agttctgcaa aatgcactac atcctgagca
    28741 ccgagaatgg agtggtcgcc gccgcgtcct ttggcgatat cgtgaaacag cgcggcgatg
    28801 aaaatcagct cagttgacgg caggcgcggc cagacgtcca cacacaacgg atggcgctgg
    28861 cgcgtttctt cactggcaaa actctccagt ttcagcatca cgcggatagt atgttcatcc
    28921 accgtgtagg cgtggaacag atcaaactgc atctgcccga cgatatgcga ccattgcggc
    28981 atatacgcgc cgagcacgct atggcgatgc attggcaata gtccgcgccg caccgctccg
    29041 gggtgacgca gaatgctcaa aaacagtttt cgtgcttgcg gaatattaca cagcggttgt
    29101 tgcagatggc gacgggcatg gcgtaactgg cgcagcgtgg tggagtaaat gccggtgatc
    29161 gcactgttgc gcaccatggt gtagaacata cgcaagatgg cttccggctg gcgcataaat
    29221 agtgtttcat cacgcaggtc gattagcgta ccgcgtagct gaaactcatc gtcgagtgga
    29281 cgtggttttt cgtcggcggg aagggcgagg atggcttcat cgaacagttg cagcagcatc
    29341 tggttgagtt cactgacgcg gcgtgtaacg cggaagtaat ccttcatcat ccgctcgacc
    29401 ggttcgttgc cttcaccact gtaattcaga cgctgagcga cgctaagttg gcgatcgaat
    29461 aacagacgat tatcgtaacg gctgacgacc agatgcaggg caaagcgaat acgccacagt
    29521 atatgcagac attcgtttaa ttccgcccgc tccgctgagg ttaagaagcc aaacccgacc
    29581 atttcatcca gcgatgttgc gccaaaatga cggcgggcca cccattgcag agtgtgaata
    29641 tcgcgcaagc cgccagggct acttttgatg tctggttcaa ggttgtagct ggtgccatgg
    29701 taacgctgat ggcgctggtt ctgttcttca actttcgctg cgtagaactt gtcggaaggc
    29761 cagaatcctt cgctaaaaat atgtttttgc agttcgagga agagcgcaac atcgccaatt
    29821 aataagcggg attcgattaa attggtggcg acggttaaat ccgataaccc ttccaacatg
    29881 cactcttcaa gcgtgcgcac actatgaccg acttccagct ttacatccca gagcagcgtt
    29941 aacagctcgc ccactttttg cgcctgatcg tccgggagct ttttacggct taaaatcagt
    30001 aaatcgatgt cagaaagtgg atgcagctcg ccacgaccgt agccaccgac ggcaaccaat
    30061 gccaggtcgg caatctggct gaatcccgct tcaatccata atcgttgcag gagctggtct
    30121 atgaactcgg tacgtgcctc aatcaactgt tctgcagaga tcccattgtc aaaggcatca
    30181 cccagccaac gctggaaagt atcgatatgg gcttttatcc cacagacggt taattcatca
    30241 cggggccagg cgcatggatt ttgcggttga ccgggcaggg tggagagagc ggtgtttgcg
    30301 tactgttctg gaagggtatt cattgtgcgc cacccataag attaaattat cgcattaaaa
    30361 aagccggcat tcgccggctt catcttattc gtcgtgcgag attatcgccg ggatggtgtc
    30421 atccttgcgt agcgtcaaaa tttcgcagcc gttatcagtc accacaatag tatgctcata
    30481 ttgtgcagac aagctgcgat ctttggtttt taccgtccag ccatctttca tggtgcggat
    30541 ctctttttta cccgcgttga ccattggctc aatggtgaac gtcatccccg gtttcagcac
    30601 gacgttggtt tcacgggagt catagtgcag cacctgcggt tcttcatgga agccgcgacc
    30661 aataccgtgt ccgcaatatt cacgaacgac ggagaagcct tctgcttcga caaatttctg
    30721 aatcgccgca ccgatttcgc gcagattaat gcctggtttt accatgcgta gcgccaggta
    30781 caggctttct tgcgtgatgc ggcacaggcg ttcgcccatg atggtcggct taccgacgat
    30841 aaacattttc gaggtatcgc cgtggaaacc atctttgatt acggtgacat caatgttaac
    30901 gatatcgcca tctttcagca gcttagcatc gtccgggata ccgtggcaca ccacttcatt
    30961 aatagagatg caaacggatt tcggatagcc gtgatagccg aggcaggcag aaaccgcgtg
    31021 ttgttcatta acaatgtaat cattacagat gcgatccagc tcgccggtgc tgacgcccgg
    31081 tttaacatac ggttcgatca tctccagcac ttcggcagcc agtcggccag cgacgcgcat
    31141 tttttcgata tcttctgggg tcttgattga gatagccatt aattctgtcc atcagcgtcg
    31201 gtgataccga caatatatat gtaagtgccg tcaatggtat cacacccggg caaattgaga
    31261 atcattctga atttcgccaa acgtgccact gaaggttttc tataatagaa aattcgacgt
    31321 ctgatgctgt acacagcgcc aacaattatt ggtgtccacg acgtatttgt ggtataaagc
    31381 gcgccggact tccgatccat ttcgtataca cagactggac ggaagcgaca atctcacttt
    31441 gtgtaacaac acacacgtat cggcacatat tccggggtgc cctttggggt cggtaatatg
    31501 ggatacgtgg aggcataacc ccaactttta tatagaggtt ttaatcatgg caactgtttc
    31561 catgcgcgac atgctcaagg ctggtgttca cttcggtcac cagacccgtt actggaaccc
    31621 gaaaatgaag ccgttcatct tcggtgcgcg taacaaagtt cacatcatca accttgagaa
    31681 aactgtaccg atgttcaacg aagctctggc tgaactgaac aagattgttt ctcgcaaagg
    31741 taaaatcctt ttcgttggta ctaaacgcgc tgcaagcgaa gcggtgaaag acgctgctct
    31801 gagctgcgac cagttcttcg tgaaccatcg ctggctgggc ggtatgctga ctaactggaa
    31861 aaccgttcgt cagtccatca aacgtctgaa agacctggaa actcagtctc aggacggtac
    31921 tttcgacaag ctgaccaaga aagaagcgct gatgcgcact cgtgagctgg agaaactgga
    31981 aaacagcctg ggcggtatca aagacatggg cggtctgccg gacgctctgt ttgtaatcga
    32041 tgctgaccac gaacacattg ctatcaaaga agcaaacaac ttgggtattc cggtatttgc
    32101 tatcgttgat accaactctg atccggacgg tgttgacttc gttatcccgg gtaacgacga
    32161 cgcaatccgt gctgtgaccc tgtacctggg cgctgttgct gcaaccgtac gtgaaggccg
    32221 ttctcaggat ctggcttccc aggcggaaga aagcttcgta gaagctgagt aataaggctt
    32281 gataactccc ccaaaatagt tcgagttgca gaaaggcggc aagctcgaga attcccggga
    32341 gcttacatca gtaagtgacc gggatgagcg agcgaagaca acgcatctgc ggcgcgaaat
    32401 atgaaggggg agagccctta tagaccaggt agtacacgtt tggttagggg gcctgcatat
    32461 ggcccccttt ttcactttta tatctgtgcg gtttaatgcc gggcagatca catctccgag
    32521 gattttagaa tggctgaaat taccgcatcc ctggtaaaag agctgcgtga gcgtactggc
    32581 gcaggcatga tggattgcaa aaaagcactg actgaagcta acggcgacat cgagctggca
    32641 atcgaaaaca tgcgtaagtc cggtgctatt aaagcagcga aaaaagcagg caacgttgct
    32701 gctgacggcg tgatcaaaac caaaatcgac ggcaactacg gcatcattct ggaagttaac
    32761 tgccagactg acttcgttgc aaaagacgct ggtttccagg cgttcgcaga caaagttctg
    32821 gacgcagctg ttgctggcaa aatcactgac gttgaagttc tgaaagcaca gttcgaagaa
    32881 gaacgtgttg cgctggtagc gaaaattggt gaaaacatca acattcgccg cgttgctgcg
    32941 ctggaaggcg acgttctggg ttcttatcag cacggtgcgc gtatcggcgt tctggttgct
    33001 gctaaaggcg ctgacgaaga gctggttaaa cacatcgcta tgcacgttgc tgcaagcaag
    33061 ccagaattca tcaaaccaga agacgtatcc gctgaagtgg tagaaaaaga ataccaggta
    33121 cagctggata tcgcgatgca gtctggtaag ccgaaagaaa tcgcagagaa aatggttgaa
    33181 ggccgcatga agaaattcac cggcgaagtt tctctgaccg gtcagccgtt cgttatggaa
    33241 ccaagcaaaa ctgttggtca gctgctgaaa gagcataacg ctgaagtgac tggcttcatc
    33301 cgtttcgaag tgggtgaagg catcgagaaa gttgagactg actttgcagc agaagttgct
    33361 gcgatgtcca agcagtctta attatcaaaa aggagccgcc tgagggcggc ttctttttgt
    33421 gcccatcttg taaattcagc taacccttgt ggggctgcgc tgaaaagcga cgtacaatgt
    33481 cgctagtatt aattcatttc aatcgttgac agtctcagga aagaaacatg gctaccaatg
    33541 caaaacccgt ctataaacgc attctgctta agttgagtgg cgaagctctg cagggcactg
    33601 aaggcttcgg tattgatgca agcatactgg atcgtatggc tcaggaaatc aaagaactgg
    33661 ttgaactggg tattcaggtt ggtgtggtga ttggtggggg taacctgttc cgtggcgctg
    33721 gtctggcgaa agcgggtatg aaccgcgttg tgggcgacca catggggatg ctggcgaccg
    33781 taatgaacgg cttggcaatg cgtgatgcac tgcaccgcgc ctatgtgaac gctcgtctga
    33841 tgtccgctat tccattgaat ggcgtgtgcg acagctacag ctgggcagaa gctatcagcc
    33901 tgttgcgcaa caaccgtgtg gtgatcctct ccgccggtac aggtaacccg ttctttacca
    33961 ccgactcagc agcttgcctg cgtggtatcg aaattgaagc cgatgtggtg ctgaaagcaa
    34021 ccaaagttga cggcgtgttt accgctgatc cggcgaaaga tccaaccgca accatgtacg
    34081 agcaactgac ttacagcgaa gtgctggaaa aagagctgaa agtcatggac ctggcggcct
    34141 tcacgctggc tcgtgaccat aaattaccga ttcgtgtttt caatatgaac aaaccgggtg
    34201 cgctgcgccg tgtggtaatg ggtgaaaaag aagggacttt aatcacggaa taattcccgt
    34261 gatggataaa taagggtaag attccgcgta agtatcgcgg gggcgtaagt ctggttataa
    34321 ggcgttattg ttgcaggcag tttggtcacg gccagcgcgc agcaaccgga gcgtacaaaa
    34381 gtacgtgagg atggcgagca ctgctcgggg ccaaaatggc aaataaaata gcctaataat
    34441 ccagacgatt acccgtaata tgtttaatca gggctatact tagcacactt ccactgtgtg
    34501 tgactgtctg gtctgactga gacaagtttt caaggattcg taacgtgatt agcgatatca
    34561 gaaaagatgc tgaagtacgc atggacaaat gcgtagaagc gttcaaaacc caaatcagca
    34621 aaatacgcac gggtcgtgct tctcccagcc tgctggatgg cattgtcgtg gaatattacg
    34681 gcacgccgac gccgctgcgt cagctggcaa gcgtaacggt agaagattcc cgtactctga
    34741 aaatcaacgt gtttgatcgt tcaatgtctc cggccgttga aaaagcgatt atggcctccg
    34801 atctcggcct gaacccgaac tctgcgggta gcgacatccg tgttccgctg ccgccgctga
    34861 cggaagaacg tcgtaaagat ctgaccaaaa tcgttcgtgg tgaagcagaa caagcgcgtg
    34921 ttgcagtacg taatgtgcgt cgtgacgcga acgacaaagt gaaagcactg ttgaaagata
    34981 aagagatcag cgaagacgac gatcgccgtt ctcaggacga tgtacagaaa ctgactgatg
    35041 ctgcaatcaa gaaaattgaa gcggcgctgg cagacaaaga agcagaactg atgcagttct
    35101 gatttcttga acgacaaaaa cgccgctcag tagatccttg cggatcggct ggcggcgttt
    35161 tgctttttat tctgtctcaa ctctggatgt ttcatgaagc aactcaccat tctgggctcg
    35221 accggctcga ttggttgcag cacgctggac gtggtgcgcc ataatcccga acacttccgc
    35281 gtagttgcgc tggtggcagg caaaaatgtc actcgcatgg tagaacagtg cctggaattc
    35341 tctccccgct atgccgtaat ggacgatgaa gcgagtgcga aacttcttaa aacgatgcta
    35401 cagcaacagg ggagccgcac cgaagtctta agtgggcaac aagccgcttg cgatatggca
    35461 gcgcttgagg aggttgatca ggtgatggca gccattgttg gtgctgctgg gctgttacct
    35521 acgcttgctg cgatccgcgc gggtaaaacc attttgctgg ccaataaaga atcactggtt
    35581 acctgcggac gtctgtttat ggacgccgta aagcagagca aagcgcaatt gttaccggtc
    35641 gatagcgaac ataacgctat ttttcagagt ttaccgcaac ctatccagca taatctggga
    35701 tacgctgacc ttgagcaaaa tggcgtggtg tccattttac ttaccgggtc tggtggccct
    35761 ttccgtgaga cgccattgcg cgatttggca acaatgacgc ccgatcaagc ttgccgtcat
    35821 ccgaactggt cgatgggacg taaaatttct gtcgattcgg ctaccatgat gaacaaaggt
    35881 ctggaataca ttgaagcgcg ttggctgttt aacgccagcg ccagccagat ggaagtgctg
    35941 attcacccgc agtcagtgat tcactcaatg gtgcgctatc aggacggcag tgttctggcg
    36001 cagctggggg aaccggatat gcgtacgcca attgcccaca ccatggcatg gccgaatcgc
    36061 gtgaactctg gcgtgaagcc gctcgatttt tgcaaactaa gtgcgttgac atttgccgca
    36121 ccggattatg agcgttatcc atgcctgaaa ctggcgatgg aggcgttcga acaaggccag
    36181 gcagcgacga cagcattgaa tgccgcaaac gaaatcaccg ttgctgcttt tcttgcgcaa
    36241 caaatccgct ttacggatat cgctgcgttg aatttatccg tactggaaaa aatggatatg
    36301 cgcgaaccac aatgtgtgga cgatgtgtta tctgttgatg cgaacgcgag tgaagtcgcc
    36361 agaaaagagg tgatgcgtct cgcaagctga ggataatccg gctacagaga gtcgcgctat
    36421 ttgttagcgt agggcttcag tgatatagtc tgcgccatct gatcgtaagt agttggcttt
    36481 ataaggtcag gtatgccgtg gttttacacg gctttttttt gtataggctt cagtattcct
    36541 gagtaccgta aaccctgtca gggaataaaa aacgcgtgat gttgtctgct actcaaccac
    36601 ttagcgaaaa attgccagcg catggctgcc gtcatgttgc gatcattatg gacggcaatg
    36661 gtcgctgggc aaaaaagcaa gggaagattc gtgcctttgg gcataaagcc ggggcaaaat
    36721 ccgtccgccg ggctgtctct tttgcggcca acaacggtat tgaggcgtta acgctgtatg
    36781 cctttagtag tgaaaactgg aaccgaccag cgcaggaagt cagtgcgtta atggaactgt
    36841 ttgtgtgggc gctcgatagc gaagtaaaaa gtctgcaccg acataacgtg cgtctgcgta
    36901 ttattggcga taccagtcgt tttaactcgc gtttgcaaga acgtattcgt aaatctgaag
    36961 cgctaacagc cggaaatacc ggtctgacgc tgaatattgc ggcgaactac ggtggacgtt
    37021 gggatatagt ccagggagtc aggcaactgg ctgaaaaggt gcagcaagga aacctgcaac
    37081 cagatcagat agatgaagag atgttaaacc agcatgtctg tatgcatgaa ctggcccctg
    37141 tagatttagt aattaggact gggggagagc atcgcattag taactttttg ctttggcaaa
    37201 ttgcctatgc cgaactttac tttacagatg ttctctggcc cgatttcgat gaacaagact
    37261 ttgaaggggc gttaaatgcc tttgctaatc gagagcgtcg tttcggcggc accgagcccg
    37321 gtgatgaaac agcctgatgg gggtcgcttt tgctgaagta tcgcctgata tctgcttttg
    37381 tgttaatacc cgtcgtcatc gcggcgttgt ttctgttgcc gccggtgggg ttcgccattg
    37441 taacgctggt ggtctgcatg ctggcagcgt gggaatgggg acagcttagc ggttttacca
    37501 ctcgttcgca gcgagtatgg ttggcggtgt tatgcgggtt attgttggcg ctgatgcttt
    37561 ttctgttgcc ggaatatcac cgaaatattc atcaaccgct ggttgaaatc tcactttggg
    37621 cttcgttggg ttggtggatt gtcgcgctat tgctggtgct gttttaccca ggttccgcag
    37681 caatctggcg taactctaaa acattgcgcc ttatttttgg cgtgctaacc attgttccct
    37741 tcttctgggg catgctggcg ttacgggcct ggcactatga cgagaatcat tacagtggcg
    37801 caatatggct gctctatgtc atgatcctgg tatggggcgc tgactccggc gcatatatgt
    37861 ttggcaaatt gtttggtaaa cataagctgg caccgaaggt ttctccgggt aaaacctggc
    37921 aaggctttat aggtggactc gctactgcag cggtaatctc atggggttat ggcatgtggg
    37981 cgaatctcga cgtcgctccc gtcaccttac tcatttgctc tattgtcgca gcgttagcct
    38041 cagtgctcgg cgatctgacc gagagtatgt ttaagcgtga agcaggaatt aaggacagcg
    38101 gtcatttaat tccaggacac ggtggtattt tagatcgtat tgatagcctg acggctgcgg
    38161 taccggtctt tgcttgcttg ttgttactgg tattcaggac gctttaacgg aaggtaatat
    38221 gctgagtttt ctctgggatt tggcttcgtt catcgttgca ctgggtgtac ttatcaccgt
    38281 gcatgaattt ggtcatttct gggttgcccg gcgttgtggt gttcgcgttg agcgtttctc
    38341 aatagggttt ggtaaggcgc tctggcggcg aactgataag ctcggcaccg aatatgttat
    38401 cgccctgatc ccgttgggcg gttatgtcaa aatgctggat gagcgcgcag aaccggtcgt
    38461 tccggaactc cgccaccatg ccttcaataa taaatctgtc ggccaacgag cggcgattat
    38521 tgccgcaggt ccggttgcaa acttcatttt tgctatcttt gcctactggc tggtttttat
    38581 tattggtgtg cctggcgtac gtccggtggt tggcgaaata gcagccaatt cgatagctgc
    38641 ggaagcacaa attgcaccag gtacggaact aaaagccgta gatggtatcg aaacgcctga
    38701 ttgggatgcc gtgcgtttgc agttggtcga taaaattggc gatgaaagca ccaccattac
    38761 agtagcgcca tttgacagcg accaacggcg ggatgtaaag ctcgatttac gtcactgggc
    38821 gtttgagcct gataaagaag atccggtatc ttcgctgggg attcgtcctc gtgggccgca
    38881 aattgaacct gtactggaaa atgtgcagcc aaactcggcg gcaagcaagg caggtttgca
    38941 agcaggcgac aggatcgtta aagtcgatgg tcagccctta acgcagtggg tgacctttgt
    39001 gatgcttgtc cgggataacc cgggtaaatc cttagcgtta gaaatcgaaa ggcaggggag
    39061 tcccttgtct ttgacattaa tcccggagag taaaccgggt aatggtaaag cgattggttt
    39121 tgtcggtatt gagccgaaag tcattccttt gccagatgag tataaagttg tacgccagta
    39181 tgggccgttc aacgccatcg tcgaagccac ggacaaaacg tggcagctga tgaagctgac
    39241 ggtcagtatg ctgggaaaat tgatcaccgg tgatgtgaaa ctgaacaacc tcagtgggcc
    39301 gatctctatc gccaaggggg ctgggatgac agcggaactc ggggtagttt attacctgcc
    39361 gtttcttgcg cttattagcg tgaacttagg gataattaac ctgtttccgt tgcccgtact
    39421 tgacgggggg catctgctgt tccttgcgat cgaaaagatc aagggcggac cggtatccga
    39481 gcgggttcaa gacttttgtt atcgcattgg ctcgattctg ctggtgctgt taatggggct
    39541 tgcacttttc aatgatttct ctcggttatg agagttagtt aggaagaacg cataataacg
    39601 atggcgatga aaaagttgct catagcgtcg ctgctgttta gcagcgccac cgtatacggt
    39661 gctgaagggt tcgtagtgaa agatattcat ttcgaaggcc ttcagcgtgt cgccgttggt
    39721 gcggccctcc tcagtatgcc ggtgcgtaca ggcgacacgg ttaatgatga agatatcagt
    39781 aataccattc gcgctctgtt tgctaccggc aactttgagg atgttcgcgt ccttcgtgat
    39841 ggtgataccc ttctggttca ggtaaaagaa cgtccgacca ttgccagcat tactttctcc
    39901 ggtaacaaat cggtgaaaga tgacatgctg aagcaaaacc tcgaggcttc tggtgtgcgt
    39961 gtgggcgaat ccctcgatcg caccaccatt gccgatatcg agaaaggtct ggaagacttc
    40021 tactacagcg tcggtaaata tagcgccagc gttaaagctg tcgtgacccc gctgccgcgc
    40081 aaccgtgttg acctaaaact ggtgttccag gaaggtgtgt cagctgaaat ccagcaaatt
    40141 aacattgttg gtaaccatgc tttcaccacc gacgaactga tctctcattt ccaactgcgt
    40201 gacgaagtgc cgtggtggaa tgtggtaggc gatcgtaaat accagaaaca gaaactggcg
    40261 ggcgatcttg aaaccctgcg cagctactat ctggaccgcg gttatgcccg tttcaacatc
    40321 gactctaccc aggtcagtct gacgccagat aaaaaaggta tttacgtcac ggtgaacatc
    40381 accgaaggcg atcagtacaa gctttctggc gttgaagtga gcggcaacct tgccgggcac
    40441 tccgctgaaa ttgagcaact gactaagatc gagccgggcg agctgtataa cggcactaaa
    40501 gtgaccaaaa tggaagatga cattaaaaag cttctcggtc gctatggtta tgcctatccg
    40561 cgcgtacagt cgatgcctga aattaacgat gccgacaaaa ccgttaaatt acgcgtgaac
    40621 gttgatgcgg gtaaccgttt ctacgtgcgt aagatccgtt ttgaaggtaa cgatacctcg
    40681 aaagatgccg tcctgcgtcg cgaaatgcgt cagatggaag gtgcatggtt ggggagcgat
    40741 ctggtcgatc agggtaagga gcgtctgaat cgtctgggct tctttgaaac tgtcgatacc
    40801 gatacccaac gtgttccggg tagcccggac caggttgatg tagtctacaa ggtgaaagaa
    40861 cgtaacactg gtagcttcaa cttcgggatt ggttacggta ctgaaagtgg cgtgagcttc
    40921 caggctggcg tgcaacagga taactggtta ggtacaggtt atgctgttgg tatcaacggg
    40981 accaaaaacg attaccagac ctatgctgaa ctgtcggtaa ccaacccgta cttcaccgta
    41041 gatggcgtaa gcctcggtgg tcgtctcttc tataatgact tccaggcaga tgacgccgac
    41101 ctgtccgact ataccaacaa gagttatggt acagacgtga cgttgggctt cccgattaac
    41161 gaatataact cgctgcgtgc aggtctgggt tatgtacata actccctgtc caacatgcag
    41221 cctcaggttg cgatgtggcg ttatctgtac tctatgggtg aacatccgag cacctctgat
    41281 caggataaca gcttcaaaac ggacgacttc acgttcaact atggttggac ctataacaag
    41341 cttgaccgtg gttacttccc gacagatggt tcacgtgtca acctgaccgg taaagtgacc
    41401 attcctggat cggataacga atactacaaa gtgacgttag acacggcgac ttatgtgccg
    41461 atcgatgacg atcacaaatg ggttgttctg ggtcgtaccc gctggggtta tggtgatggt
    41521 ttaggcggca aagagatgcc gttctacgag aacttctatg ccggtggttc cagcaccgtg
    41581 cgtggcttcc agtccaatac cattggtccg aaagcagttt acttcccgca tcaggccagt
    41641 aattatgatc cggactatga ttacgaatgt gcgactcagg acggcgcgaa agacctgtgt
    41701 aaatcggatg atgctgtagg cggtaacgcc atggcggttg ccagcctcga gttcatcacc
    41761 ccgacgccgt ttattagcga taagtatgct aactcggttc gtacttcatt cttctgggat
    41821 atgggtaccg tttgggatac aaactgggat tccagccaat attctggata tccggactat
    41881 agtgatccaa gcaatatccg tatgtctgcg ggtatcgcat tacaatggat gtccccattg
    41941 gggccgttgg tgttctccta cgcccagccg ttcaaaaagt acgatggaga caaggcagaa
    42001 cagttccagt ttaacatcgg taaaacctgg taagtgttct ccacaaagga atgtagtggt
    42061 agtgtagcga tgactttagg cgatcaatat aagatcgccg ggccacgcaa agaactgcac
    42121 cctccggtgc aaatgggatg gtaaggagtt tattgtgaaa aagtggttat tagctgcagg
    42181 tcttggttta gcactggcaa cttctgctca ggcggctgac aaaattgcaa tcgtcaatat
    42241 gggcagcctg ttccagcagg tagcgcagaa aaccggtgtt tctaacacgc tggaaaatga
    42301 gttcaaaggc cgtgccagcg aactgcagcg tatggaaacc gatctgcagg ctaaaatgaa
    42361 aaaactgcag tccatgaaag cgggcagcga tcgcaccaaa ctggaaaaag acgtgatggc
    42421 tcagcgccag acttttgctc agaaagcgca ggcttttgag caggatcgcg cacgtcgttc
    42481 caacgaagaa cgcggcaaac tggttactcg tatccagact gctgtgaaat ccgttgccaa
    42541 cagccaggat atcgatctgg ttgttgatgc aaacgccgtt gcttacaaca gcagcgatgt
    42601 aaaagacatc actgccgacg tactgaaaca ggttaaataa gtaatgcctt caattcgact
    42661 ggctgattta gcgcagcagt tggatgcaga actacacggt gatggcgata tcgtcatcac
    42721 cggcgttgcg tccatgcaat ctgcacaaac aggtcacatt acgttcatgg ttaacccaaa
    42781 ataccgtgag catttaggct tgtgccaggc gtccgcggtt gtcatgaccc aggacgatct
    42841 tcctttcgcg aaaagtgccg cgctggtagt gaagaatccc tacctgactt acgcgcgcat
    42901 ggcgcaaatt ttagatacca cgccgcagcc cgcgcagaac attgcaccca gtgcggtgat
    42961 cgacgcgacg gcgaagctgg gtaacaacgt atcgattggc gctaacgcgg tgattgagtc
    43021 cggcgttgaa ctgggcgata acgtgattat cggtgccggt tgcttcgtag gtaaaaacag
    43081 caaaatcggt gcaggttcgc gtctctgggc gaacgtaacc atttaccatg agatccagat
    43141 cggtcagaat tgcctgatcc agtccggaac agtggtaggc gcagacggct ttggttatgc
    43201 caacgatcgt ggtaactggg tgaagatccc acagattggt cgcgtaatta ttggcgatcg
    43261 cgtggagatc ggtgcctgca caaccatcga tcgcggcgcg ctggatgaca ctgttattgg
    43321 caatggcgtg atcattgata accagtgcca gattgcacat aacgtcgtga ttggcgacaa
    43381 tacggcggtt gccggtggcg tcattatggc gggcagcctg aaaattggtc gttactgcat
    43441 gatcggcgga gccagcgtaa tcaacgggca tatggaaata tgcgacaaag tgacggttac
    43501 gggcatgggt atggtgatgc gtcccatcac tgaaccaggc gtctattcct caggcattcc
    43561 gctgcaaccc aacaaagtct ggcgcaaaac cgctgcactg gtgatgaaca ttgatgacat
    43621 gagcaagcgt ctgaaatcgc ttgagcgcaa ggttaatcaa caagactaac gttccatctt
    43681 ttgttcgcca aactttacgg cctgtctcat tcttacgatt gcggcaggcc gtgttattat
    43741 tgtcgtttct tatattttga caggaagagt atcttgacta ctaacactca tactctgcag
    43801 attgaagaga ttttagaact tctgccgcac cgtttcccgt tcttactggt ggatcgcgtg
    43861 ctggattttg aagaaggtcg ttttctgcgc gcagtaaaaa atgtctctgt caatgagcca
    43921 ttcttccagg gccatttccc tggaaaaccg attttcccgg gtgtgctgat tctggaagca
    43981 atggcacagg caacaggtat tctggcgttt aaaagcgtag gaaaactgga accgggtgag
    44041 ctgtactact tcgctggtat tgacgaagcg cgcttcaagc gcccggtcgt gcctggcgat
    44101 caaatgatca tggaagtcac tttcgaaaaa acgcgccgcg gcctgacccg ttttaaaggg
    44161 gttgctctgg tcgatggtaa agtagtttgc gaagcaacga tgatgtgtgc tcgtagccgg
    44221 gaggcctgat acgtgattga taaatccgcc tttgtgcatc caaccgccat tgtggaagag
    44281 ggcgcgtcaa ttggcgcgaa cgcacacatt ggtccttttt gtatcgttgg accccatgtc
    44341 gaaattggtg agggtaccgt actgaaatct cacgttgtcg tgaatggtca tactaaaatt
    44401 ggccgcgata atgagattta tcagtttgcc tccatcggcg aagttaacca ggatttgaaa
    44461 tatgctggcg aaccgacccg tgtggaaatc ggcgatcgta accgcattcg cgaaagcgtc
    44521 accattcatc gtggcacagt ccagggcggt ggattgacga aggtgggcag cgacaactta
    44581 ctgatgatca acgcgcacat tgcgcacgat tgtacggtag gtaaccgctg tattctcgcc
    44641 aacaacgcaa cgctggcggg tcacgtatcg gttgacgact tcgcgatcat cggcggcatg
    44701 accgcagtcc atcagttctg catcattggt gcgcatgtga tggttggcgg ctgctccggt
    44761 gtggcgcagg acgtccctcc ttatgttatt gcgcagggta accacgcaac gccgttcggt
    44821 gtcaatatcg aagggctgaa gcgccgcgga ttcagccgtg aggcgattac cgctatccgc
    44881 aatgcgtata agctaattta tcgtagcggt aaaacgctcg atgaagtgaa accggaaatt
    44941 gctgaactgg cggaaacata tccggaagtg aaagccttta ccgatttctt tgcacgctca
    45001 acgcgcggtc tgattcgtta atgactgacc agcgtccatt aacgattgcc ctggtcgccg
    45061 gagaaacctc cggcgatatc ctgggggccg gtttaatccg cgctctgaaa gaacgtgtgc
    45121 ccaacgcccg ctttgttggt gttgccgggc cacgaatgca ggctgaaggc tgcgaagcct
    45181 ggtacgaaat ggaagaactg gcggtgatgg gcattgttga agtgctcggt cgtctgcgtc
    45241 gcttactgca tattcgtgcc gatctgacaa agcgttttgg cgaactgaag ccagatgttt
    45301 ttgttggtat tgatgcgccc gacttcaata ttactcttga aggtaacctc aaaaagcagg
    45361 gtatcaaaac cattcattac gtcagtccat ccgtctgggc gtggcgacag aaacgcgttt
    45421 tcaaaatagg cagagccacc gatctggtgc tcgcatttct gcctttcgaa aaagcgtttt
    45481 atgacaaata caatgtaccg tgccgcttta tcggtcatac catggctgat gccatgccgt
    45541 tagatccaga taaaaatggt gcccgtgatg tgctggggat cccttacgat gcccactgtc
    45601 tggcattgtt gccgggcagc cgtggtgcag aagtcgaaat gcttagtgcc gatttcttga
    45661 aaactgccca gcttttgcgc cagacatatc cggatctcga aatcgtggtg ccgctggtga
    45721 atgccaaacg ccgcgagcag tttgaacgca tcaaagctga agtcgcgcca gacctgtcgg
    45781 ttcatttgct ggatggaatg ggccgtgagg cgatggtcgc cagcgatgcg gcactactgg
    45841 cgtcggggac ggcagccctg gagtgtatgc tggcgaaatg cccgatggtg gtgggatatc
    45901 gcatgaagcc ttttaccttc tggttggcga agcggctggt gaaaactgat tatgtctcgc
    45961 tgccaaatct gctggcgggc agagagttag tcaaagagtt attgcaggaa gagtgtgagc
    46021 cgcaaaaact ggctgcggcg ctgttaccgc tgttggcgaa cgggaaaacc agccacgcga
    46081 tgcacgatac cttccgtgaa ctgcatcagc agatccgctg caatgccgat gagcaggcgg
    46141 cacaagccgt tctggagtta gcacaatgat cgaatttgtt tatccgcaca cgcagctggt
    46201 tgcgggtgtg gatgaagtcg gacgcgggcc gttagttggc gcggtcgtca ccgctgcggt
    46261 gatccttgac ccggcgcgcc cgattgccgg gctgaatgat tccaaaaagc tgagcgaaaa
    46321 acgccgtctg gcgctctatg aagagatcaa agagaaagcg ttgagctgga gtctgggccg
    46381 cgcggaaccc cacgaaatcg acgagctgaa cattcttcat gcgaccatgc tggcgatgca
    46441 gcgtgccgtc gctgggctgc atattgcgcc ggaatatgtg ttgattgatg gtaaccgctg
    46501 cccgaaatta ccgatgcctg cgatggctgt ggtgaaaggg gatagccgcg taccagaaat
    46561 cagtgccgcg tctatcctgg cgaaagtgac gcgtgacgcc gaaatggcgg cgctggatat
    46621 tgttttcccg caatatggtt ttgcccaaca taaagggtat ccaaccgctt ttcatctgga
    46681 aaaactggct gaatatggcg cgactgaaca tcatcgtcgc agctttgggc ctgtcaaacg
    46741 cgcactggga cttgcgtcct gattcttgtg tcgagattaa gtaaaccgga atctgaagat
    46801 gtctgaacca cgtttcgtac acctgcgggt gcacagcgac tactcgatga tcgatggctt
    46861 ggccaaaacc gcaccgctgg taaaaaaggc ggcggcgttg ggtatgccag cactggcgat
    46921 caccgatttc accaaccttt gcggtctggt gaagttctac ggagcgggac atggcgcagg
    46981 gattaagcct atcgtcgggg cagattttaa tgtccagtgc gacctgctgg gtgatgagtt
    47041 aacccacctg acggtactgg cggcgaacaa taccggctat cagaatctgg cgttgctgat
    47101 ctcaaaagcg tatcagcgcg ggtacggtgc cgccgggccg atcatcgatc gcgactggct
    47161 tatcgaatta aacgaagggt tgatccttct ttccggcggg cgcatgggcg acgtcggacg
    47221 cagtcttttg cgtggtaaca gcgcgctggt agatgagtgt gtcgcgtttt atgaagaaca
    47281 cttcccggat cgctattttc tcgagctgat ccgcaccggc aggccggatg aagaaagcta
    47341 tctgcacgcg gcggtggaac tggcggaagc gcgcggtttg cccgtcgtgg cgactaacga
    47401 tgtgcgcttt atcgacagca gcgactttga cgcacacgaa atccgcgtcg cgatccacga
    47461 cggctttacc ctcgacgatc ctaaacgccc gcgtaactat tcgccgcagc aatatatgcg
    47521 tagcgaagag gagatgtgtg agctgtttgc cgacatccca gaagcccttg ccaacaccgt
    47581 tgagatcgcc aaacgctgta acgtaactgt gcgtcttggt gaatacttcc tgccgcagtt
    47641 cccgaccggg gacatgagca ccgaagatta tctggtcaag cgtgcaaaag aaggcctgga
    47701 agagcgtctg gcatttttat tccctgacga ggaagaacgt cttaaacgcc gcccggaata
    47761 tgacgaacgt ctggagactg aacttcaggt tatcaaccag atgggcttcc cgggctactt
    47821 cctcatcgtt atggaattta tccagtggtc gaaagataac ggcgtaccgg tagggccagg
    47881 ccgtggctcc ggtgcgggtt cactggtggc ctacgcgctg aaaatcaccg acctcgatcc
    47941 gctggaattt gacctgctgt tcgaacgttt ccttaacccg gaacgtgtct ccatgcctga
    48001 cttcgacgtt gacttctgta tggagaaacg cgatcaggtt atcgaacacg tggcggacat
    48061 gtacggtcgt gatgcggtat cgcagattat caccttcggt acgatggcgg cgaaagcggt
    48121 gatccgcgac gtaggccgcg tgctggggca tccgtacggc tttgtcgacc gtatctcgaa
    48181 actgatcccg cccgatccgg ggatgacgct ggcgaaagcg tttgaagccg agccgcagct
    48241 gccggaaatc tacgaagcgg atgaagaagt taaggcgctg atcgacatgg cgcgcaaact
    48301 ggaaggggtc acccgtaacg ccggtaagca cgccggtggg gtggttatcg cgccgaccaa
    48361 aattaccgat tttgcgccgc tttattgcga tgaagagggc aaacatccgg tcacccagtt
    48421 tgataaaagc gacgttgaat acgccggact ggtgaagttc gacttccttg gtttgcgtac
    48481 gctcaccatc atcaactggg cgctggagat gatcaacaag cggcgggcga agaatggcga
    48541 gccaccgctg gatatcgccg cgatcccgct ggatgacaag aaaagcttcg acatgctgca
    48601 acgctcggaa accacggcgg tattccagct tgaatcgcgc ggcatgaagg acctgatcaa
    48661 gcgtctgcaa cctgactgct tcgaagatat gatcgcactg gtggcactgt tccgccctgg
    48721 tccgttgcag tcagggatgg tggataactt tatcgaccgt aaacatggtc gtgaagagat
    48781 ctcctatccg gacgtgcagt ggcagcatga aagcctgaaa tcggtactgg agccaaccta
    48841 cggcattatc ttgtatcagg aacaggtcat gcagattgcc caggtgcttt ctggttatac
    48901 cctcggtggc gcggatatgc tgcgccgtgc gatgggtaag aaaaagccgg aagagatggc
    48961 taagcagcgt tctgtatttg ctgaaggtgc agaaaagaac ggaatcaacg ccgaactggc
    49021 gatgaaaatc ttcgacctgg tggagaaatt cgcgggttac ggatttaaca aatcgcactc
    49081 tgcggcctat gctttggtgt catatcaaac gttatggctg aaagcgcact atccggcgga
    49141 gtttatggcg gcggtaatga ccgccgatat ggacaacacc gagaaggtgg tgggcctggt
    49201 ggatgagtgc tggcggatgg ggctgaaaat cctgccacca gatataaact ccggtcttta
    49261 ccatttccac gtcaacgacg atggcgaaat cgtgtatggt attggcgcca tcaaaggggt
    49321 aggtgaaggt ccgattgagg ccatcatcga agcccgtaat aaaggcggct acttccgcga
    49381 actgtttgat ctctgcgccc gtaccgacac caaaaagtta aaccggcggg tgctggaaaa
    49441 actgatcatg tccggggcgt ttgaccgtct tgggccacac cgcgcggcgc tgatgaactc
    49501 gctgggtgat gcgttaaaag cggcagatca acacgcgaaa gcggaagcta tcggtcaggc
    49561 cgatatgttc ggcgtgctgg ccgaagagcc ggaacaaatt gaacaatcct acgccagctg
    49621 ccaaccgtgg ccggagcagg tggtattaga tggggaacgt gaaacgttag gcctgtacct
    49681 gaccggacac cctatcaacc agtatttaaa agagattgag cgttatgtcg gaggcgtaag
    49741 gctgaaagac atgcacccga cagaacgtgg taaagtcatc acggctgcgg ggctcgttgt
    49801 tgccgcgcgg gttatggtca ccaagcgcgg caatcgtatc ggtatctgca cgctggatga
    49861 ccgttccggg cggctggaag tgatgttgtt tactgacgcc ctggataaat accagcaatt
    49921 gctggaaaaa gaccgcatac ttatcgtcag cggacaggtc agctttgatg acttcagcgg
    49981 tgggcttaaa atgaccgctc gcgaagtgat ggatattgac gaagcccggg aaaaatatgc
    50041 tcgcgggctt gctatctcgc tgacggacag gcaaattgat gaccagcttt taaaccgact
    50101 ccgtcagtct ctggaacccc accgctctgg gacaattcca gtacatctct actatcagag
    50161 ggcggatgca cgcgcgcggt tgcgttttgg cgcgacgtgg cgtgtctctc cgagcgatcg
    50221 tttattaaac gatctccgtg gcctcattgg ttcggagcag gtggaactgg agtttgacta
    50281 atacaggaat actatgagtc tgaatttcct tgattttgaa cagccgattg cagagctgga
    50341 agcgaaaatc gattctctga ctgcggttag ccgtcaggat gagaaactgg atattaacat
    50401 cgatgaagaa gtgcatcgtc tgcgtgaaaa aagcgtagaa ctgacgcgta aaatcttcgc
    50461 cgatctcggt gcatggcaga ttgcgcaact ggcacgccat ccgcagcgtc cttataccct
    50521 ggattacgtt cgcctggcat ttgatgaatt tgacgaactg gctggcgacc gtgcgtatgc
    50581 agacgataaa gctatcgtcg gtggtatcgc ccgtctcgat ggtcgtccgg tgatgatcat
    50641 tggtcatcaa aaaggtcgtg aaaccaaaga aaaaattcgc cgtaactttg gtatgccagc
    50701 gccagaaggt taccgcaaag cactgcgtct gatgcaaatg gctgaacgct ttaagatgcc
    50761 tatcatcacc tttatcgaca ccccgggggc ttatcctggc gtgggcgcag aagagcgtgg
    50821 tcagtctgaa gccattgcac gcaacctgcg tgaaatgtct cgccttggcg taccggtagt
    50881 ttgtacggtt atcggtgaag gtggttctgg cggtgcgctg gcgattggcg tgggcgataa
    50941 agtgaatatg ctgcaataca gcacctattc cgttatctcg ccggaaggtt gtgcgtccat
    51001 tctgtggaag agcgccgaca aagcgccgct ggcggctgaa gcgatgggta tcatcgctcc
    51061 gcgtctgaaa gagctgaaac tgatcgactc catcatcccg gaaccgctgg gcggcgctca
    51121 ccgtaacccg gaagcgatgg cggcatcgtt gaaagcgcaa ctgctggcgg atctggccga
    51181 tctcgatgtg ttaagcactg aagatttaaa aaatcgtcgt tatcagcgcc tgatgagcta
    51241 cggttacgcg taattcgcaa aagttctgaa aaagggtcac ttcggtggcc cttttttatc
    51301 gccacggttt gagcaggcta tgattaagga aggattttcc aggaggaaca catgaacatc
    51361 attgccatta tgggaccgca tggcgtcttt tataaagatg agcccatcaa agaactggag
    51421 tcggcgctgg tggcgcaagg ctttcagatt atctggccac aaaacagcgt tgatttgctg
    51481 aaatttatcg aacataaccc tcgaatttgc ggcgtgattt ttgactggga tgagtacagt
    51541 ctcgatttat gcagcgatat caatcaactt aatgaatatc tcccgcttta tgccttcatc
    51601 aacacccact cgacgatgga tgtcagcgtg caggatatgc ggatggcgct ctggtttttt
    51661 gaatatgcgc tggggcaggc ggaagatatc gccattcgta tgcgtcagta caccaacgaa
    51721 tatcttgata acattacgcc gccgttcacg aaagccttgt ttacctacgt caaagagcgg
    51781 aagtacacct tttgtacgcc ggggcatatg ggcggcaccg catatcaaaa aagcccggtt
    51841 ggctgtctgt tttatgattt tttcggcggg aataccctta aggctgatgt ctctatttcg
    51901 gtcaccgagc ttggttcgtt gcttgaccac accgggccac acctggaagc ggaagagtac
    51961 atcgcgcgga cttttggcgc ggaacagagt tatatcgtta ccaacggaat atcgacgtcg
    52021 aacaaaattg tgggtatgta cgccgcgcca tccggcagta cgctgttgat cgaccgcaac
    52081 tgccataaat cactggcgca tctgttgatg atgaacgatg tagtgccagt ctggctgaaa
    52141 ccgacgcgta atgcgttggg gattctgggg gggatcccgc gccgtgaatt tactcgcgac
    52201 agcatcgaag agaaagtcgc tgccactacg caagcacaat ggccggttca tgcggtgatc
    52261 accaactcca cctatgatgg tttgctctac aacaccgact ggatcaaaca gacgctggat
    52321 gtcccgtcga tccacttcga ttccgcctgg gtgccgtata ctcattttca tccaatctac
    52381 cagggtaaaa gtggtatgag cggcgagcgt gttgcgggaa aagttttctt cgaaacgcaa
    52441 tcgacccaca aaatgctggc ggcgttatcg caggcttcac tgatccacat taaaggcgag
    52501 tatgacgaag aggcgtttaa cgaagccttt atgatgcata ccaccacctc gcccagttat
    52561 cccattgttg cttcggttaa gacggcggcg gcgatgctgc gtggtaatcc gggcaaacgg
    52621 ctgattaacc gttcggtgga gcgggcgctg cattttcgca aagaggtcca gcggctgcgg
    52681 gaagagtctg acggttggtt tttcgatatc tggcaaccgc cgcaggtgga tgaagccgaa
    52741 tgcaggcccg ttgcgcctgg cgaacaatgg cacggcttta acgatgcgga tgccgatcat
    52801 atgtttctcg atccggttaa agtcactatt ttgacaccgg ggatggacga acagggcaat
    52861 atgagtgagg aggggatccc ggcggcgctg gtagcaaaat tcctcgacga acgtgggatc
    52921 gtagtagaga aaaccggccc ttataacctg ctgtttctct ttagtattgg catcgataaa
    52981 accaaagcaa tgggattatt gcgtgggttg acggaattta agcgctctta cgatctcaac
    53041 ctgcggatca aaaatatgct gcccgatctc tatgctgaag atcccgattt ctaccgcaat
    53101 atgcgtattc aggatctggc gcaagggatc cataagctga ttcgtaaaca cgatctttcc
    53161 ggtttgatgt tgcgggcatt cgatactttg ccggaaatga tcatgacgcc acatcaggca
    53221 tggcagagac agattaaagg tgaagtagaa accattgcgc tggaacaact ggtcggtaga
    53281 gtatcggcaa atatgatcct gccttatcca ccgggcgtac cgctgttgat gccaggagaa
    53341 atgctgacca aagagagccg cacggtactc gattttctac tgatgctttg ttccgtaggg
    53401 caacattacc ccggttttga aacggatatt cacggcgcga aacaggacga agacggcgtt
    53461 taccgcgtac gagtcctaaa aatggcggga taacttgcca gagcggcttc cgggcgagta
    53521 acgtgctgtt aacaaataaa ggagacgtta tgctgggttt aaaacaggtt caccatattg
    53581 cgattattgc gacggattat gcggtgagca aagctttcta ctgcgatatt cttggtttca
    53641 cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg gaaaggggat ttggcgctta
    53701 atgggcaata tgtgattgag cttttctctt ttccgttccc gccggagcgc cccagccgac
    53761 cggaagcttg cggcctgcgt catctggctt ttagcgttga tgacatcgat gcggcagtgg
    53821 cgcaccttga aagccataac gtgaagtgtg aagccatccg tgtcgatcca tacacgcaaa
    53881 aacgcttcac cttctttaac gatccggacg ggctgccgtt ggaactgtat gagcagtaag
    53941 gcttgtcatc gccgcatttg cccggtaacg tgccgggcat tgctactgta aaatcgcacc
    54001 atcatgacac tcacgctcaa tagacaactt ctcacctcac gccagattct ggtggccttt
    54061 agcggcgggc ttgactccac cgttctgctg catcagttgg tgcagtggcg gacggaaaat
    54121 ccgggcgtca ctctgcgcgc tattcatgtg catcacggtt taagtgccaa tgccgatgcc
    54181 tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg tgccgctggt ggtcgaacgc
    54241 gtacaacttg cgcaagaggg gctggggatt gaggcccagg cgcggcaggc acgttatcag
    54301 gcatttgccc gtaccctatt gcccggcgaa gtgctggtca ccgcgcaaca tctcgacgat
    54361 caatgtgaaa cctttctgct ggcgctaaaa cgcggtagcg gccctgccgg gctttcggct
    54421 atggcggaag tttcggagtt tgctggaaca cggcttatcc gcccgttgct tgcccgcacg
    54481 cggggggaac tggagcagtg ggcattggcg catggtttac gctggattga agacgaaagt
    54541 aatcaggacg actcatacga tcgtaacttt ctgcgcctgc gcgtagtgcc gttattgcag
    54601 cagcgttggc cgcattttgc ggaagcaacg gcccgcagcg ccacactttg tgctgaacaa
    54661 gagagtctgt tggatgaact gctggcggat gatttagcac actgccaaac gtcgcagggg
    54721 acgctgcaga ttgcgccaat gctggcgatg agtgatgccc gtcgcgcggc gattattcgc
    54781 cgctggctgg cagggcagaa tgcaccgatg ccttcccgcg acgcgctggt gcgcatctgg
    54841 caggaagtgg cgctggcgcg ggaagatgct tccccctgtt tacgtttggg cgcctttgaa
    54901 atccgccgct atcagtcgca actgtggtgg attaaatccg tcaccgggca aagcgaaacc
    54961 attgtgctgt ggcagacgtg gctacaaccg ctggaattac cggcggggct gggaaccgta
    55021 cagcttactg cgggaggcga tattcgccct ccgcgtgcag acgaagcggt cagcgtgcgt
    55081 ttcaaagcgc caggattgct gcatattgtc gggcgcaacg gcggacgtaa gctgaagaaa
    55141 atctggcaag agttaggcgt gccgccgtgg ctacgtgaca ccacgccact gctgttttat
    55201 ggagaaacgc tgattgcggc ggcaggggta tttgtgacgc aagaaggtgt ggctgaaggt
    55261 gagaatggcg taagttttgt ctggcagaaa acgcttagtt aagtgaaagc cggataagac
    55321 gcatcaaacg tcgcatccgg cgaaagtcaa tcaggattcg cttaccacca ccgtaccgat
    55381 ttcagggtgg ctaaagctgg taattttatc cagacgcagc tcgcgggttt cgccagcggc
    55441 ctcgacgacc agatactcca catttttgcg ggagactaaa tcactggctt ttgcctgcaa
    55501 tttttcgcca tctttcagct caagtgtcag cattaaatga tgctggcagg cgagctcaag
    55561 attatcgtaa tcatcacaat taattggttg atacgtatca ttcattgaca taatcgctca
    55621 ccagtaagtt tgccgcagcg tatgctgctt tttccctgac agcctcagaa agggcgtcgt
    55681 cggcagccat ttcattcagc actttcaaaa cgcagcccag cgcgtccgga acgtatccta
    55741 agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa ctcacaatat ttttccacat
    55801 gccctcctgt cagcactctg acttaaccgt ggatgcaagt ctaagcctac gaagataaac
    55861 tctgtttcgc aaggtgacta taccacactc atttctgcaa tatcagcgcc gcaactgcac
    55921 gtattccgtt acaatggcct cctgattcga aaggagtttt cttatggcgc ttaaagcgac
    55981 aatttataaa gcgacggtta atgtggccga tctcgaccgc aaccagtttc tcgatgcctc
    56041 tctgacgctg gcgcgccacc cttcagaaac ccaggagcgt atgatgctgc gcttgctggc
    56101 gtggctgaaa tatgctgatg aacgtctgca atttacccgt ggtttgtgtg ccgatgatga
    56161 gccggaagcg tggctgcgta acgatcacct gggcattgat ttgtggattg agctggggct
    56221 gccggatgag cgacgaatta agaaagcctg cacccaggcc gcagaagtgg cgctgtttac
    56281 ctataatagt cgggcggcgc aaatctggtg gcagcaaaat cagagcaaat gtgtgcagtt
    56341 tgccaatctt tccgtctggt atctggacga tgaacaactg gcgaaagtaa gcgcctttgc
    56401 cgatcgtacc atgacgctgc aggcaacgat tcaggatggt gttatctggt tatcggatga
    56461 taagaataat ctggaagtta acttaaccgc ctggcaacaa ccttcatgat tgtgatttcc
    56521 cgacatgttg ctattcccga tggtgagctt gagatcaccg ccattcgtgc gcagggcgct
    56581 ggcgggcagc atgttaataa gacctcaacg gctattcatc tgcgttttga cattcgggcg
    56641 tccagcctgc cagagtatta caaagagcgt ctgctcgccg ccagccatca tttgatcagc
    56701 agtgatggcg tgattgtcat taaggcacag gaataccgca gtcaggaact gaaccgcgaa
    56761 gccgctctgg cccggctggt ggcagtgatt aaagatttaa caaaagaaca aaaagcccga
    56821 cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc tggcatcgaa agcacaaaaa
    56881 tcaagcgtga aggcgatgcg cggcaaagta cgcagcggtc gggaataaaa agaaggaatg
    56941 gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc agcctcttta ctctgatggg
    57001 atgtaataat cgggccgaag tcgatacgct ttctccggcg caggctgccg aactgaaacc
    57061 gatgccgcaa agttggcgcg gcgtgctacc gtgtgccgat tgcgaaggaa tcgaaatctc
    57121 tctgttcctc gaaaaagacg gaacatgggt gatgaatgag cgttatctcg gtgctcgtga
    57181 agaaccttcc tccttcgctt cctacggtac atgggcgcga accgctgaca agctggtatt
    57241 aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa ggcgatgcgc tggagatgct
    57301 cgatcgtgaa ggcaatccaa ttgaatcgca gttcaactat acgctggaac cggcacaatc
    57361 cagtttacct atgacgccga tgaccctgcg aggcatgtat ttttatatgg ctgatgcggc
    57421 gaccttcact gattgcgcga ccggaaaacg tttcatggta gcgaataacg cagagctgga
    57481 gcgtagctac ctggctgcgc gcggtcacag tgaaaaaccg gtgttactgt cagtagaagg
    57541 tcactttacg cttgaggcta atccggatac tggtgcgcca accaaagtat tagcgcccga
    57601 tacggcgggt aaattttacc cgaataagga ttgcagtagt ttggggcagt aaacctgctt
    57661 aaggcagaaa caaacgcaaa attgcctgat gcgctgcgct tatcaggcct acagcatata
    57721 atgcaacatt ttgaattagc acgattttgt aggccggata aggcgtttac gccgcatccg
    57781 gcattttgtg cacgaggctg gctttatcac tgagtcagcc caacaaagcg acccgccttg
    57841 atataaatcc caggcttcag gtgcccaaca tactgtaact gcgtttctgg cttaaacgca
    57901 gagacatcac caatacgcat atgcatcaga tcggcagggc taatccagcc cgattgcgcc
    57961 agtgtaagtg gatgcccgga tttggcaaat gcatcagtga caaattccga acaaaaccac
    58021 gactttttgt ctccttcgca cacactgctt aactgcgctt tcgccaggtc gctgacgcac
    58081 tgttggcgaa aatcctcgga gaacggattc agtgagcaca tctggcgagt caccataaag
    58141 ggaataaatt cgacaatgcc gcgatagtta taaccgctat ctttgatttt attggcaaac
    58201 gcggtgatat ctgtggcttg ttgcggggta agatccggga ctcgtaagac gaaaagctta
    58261 tcactatgct tcatcgcttt tttaagggaa acaatctgga cgccagcgcc tgtcgcttct
    58321 gcaacgttat tatcacccag aaagattgca acgtgactca cagaggaagt gctgaagacg
    58381 cggattccaa atgaggttac cccaaggctt gaggagaaca gcaaatcgcc gggtttgaga
    58441 tcaggtgccg ttatttcttt tattgattgt tcggtgaaag agctttgatg ctggaatttg
    58501 acagcccatg tttttgcctc ggcatctact gctgtggctg atgggtctgg ctggctgata
    58561 tcaacggtac aagccgaaag taaaagaaag ctggggagaa gcaggcggca gtacgccttt
    58621 ggtttatcca ttttatacaa tccatgtaaa aaagggccct gaaattcagg accctttctg
    58681 gcatcagcct ttaatctgtt tcaccagata ttcgacgatg tcaccagtct taattaactg
    58741 tttctcgccg ttgcgacgat atttatattc gatatcgtcg ttgtcgaggt tacggtcgcc
    58801 cagcacaata gtgtgcggaa taccgatcag ttccatatca gcaaacatca cgcccggacg
    58861 ctctttgcgg tcatccagca gcacttcgat accttgtgca cgcagttcgc tgtacagttt
    58921 ctcagcaagc tcttgtacgc ggaaggattt gtgcatgttc atcggcagaa tcgccacctg
    58981 gaacggcgcg atagcgtcag gccatacgat gccgcgttcg tcgtagttct gctcaatcgc
    59041 cgcagctacc acacgcgtta ccccgatacc gtagcaaccc atcgtcagga tttggttacg
    59101 gccatcttca ccctgtacgg aggctttcag tgcttcggag tacttggtac ccagctggaa
    59161 gatgtgacca acttcgatac cacgtttgat cagcagcgta ccctggccat ccgggcttgg
    59221 atcgccagcc accacgttac ggatatctgc aacttccggg gtagcgacat cgcgatccca
    59281 gttgataccg aagtagtgtt taccatcgat gttagcacca gcagcgaaat cactcatcgc
    59341 cgcaacggta cggtcaatca ccaccggaat cggcatgttt accggaccca gtgaacccgg
    59401 accggcttta accacagcac gaatttcttc ttcggtcgcg aaagtcagcg ggcttgcaac
    59461 ctgcggcagt ttttctgctt taacttcgtt cagctcgtga tcaccgcgca ccagcagcgc
    59521 aaccagcggg aagctactgc cttcaaccgc tttaaccagc agagtcttaa ccgttttctc
    59581 aatcggcaga ttgaactgtt caaccagttc cgcgatggtt ttcgcgttcg gcgtatcaac
    59641 cagcgtcatt tcctgggtag cagcagcgcg cggttctttc ggcgcgatag cttctgccag
    59701 ttcaatgttc gctgcatagt cagaggtgtc ggagaagacc acatcgtctt caccgctctg
    59761 cgccagcacc tggaattcgt gagaggcgct gccgccgata gaaccggtgt cggcctgtac
    59821 ggcgcggaaa tccagcccca tgcggctgaa gattttgctg taggccgcat acattgcatc
    59881 gtaggtttcc tgcagggatt cctgagaagt atggaaagag taagcatctt tcatcaggaa
    59941 ttcgcgggaa cgcatgacgc cgaaacgcgg acgcacttcg tcgcggaact tggtctggat
    60001 ctgatagaag ttcagcggca gctgtttgta agagctaagc tcgttacgaa tcaggtcagt
    60061 gataacttct tcatgagttg ggccgagtac gaacggacgc tcgccacggt caacaaaacg
    60121 cagcagttcc ggaccgtact gttcccaacg accactctct tgccacaaat cggctggctg
    60181 aaccaccggc atcgacacct cgatcgcacc ggcgttgttc atctcttcac gcacgatgtt
    60241 ttcgactttt ttcagaacgc gcacgccggt cggcagccag gtatataacc cggaggccag
    60301 cttgcggatc atcccggcgc gcagcatcag ctgatggctg atcacctcgg cgtcggcagg
    60361 tgtctccttg agagtggaga gcaggtattg gctagtacgc atgttgttac ggttccagtt
    60421 ggaaggtaga acaggctcaa ggcgagcctg ggacaaaaaa agtgatttag tttaccagtg
    60481 caaaagaaat gtcaaaagag aagggcgtga atttaacgcg gttccagcgc aaagacttca
    60541 aaacctgcgt cggtgacgcg ccagcgaacg ttaaaatcat gtagccagac ggcataggtt
    60601 ttgcccgttt cctcaccttt acgataggcc gggcgcgggt cctgcgccag tacttcgcgg
    60661 ataaacagcg ttaactgcgg ataacgcttc tccagcgtca aaagctgctt ttcgacctct
    60721 gcggtaaaac tcaccgccat ctctgcagct ggcgcacttt gcgcatagct ggcactggca
    60781 tcgggaagcg attcggcaaa ggggagatac ggtttgatat ccactaccgg cgtaccatcg
    60841 accagatcca gactgccgag cttcagaatc acgctgcctt tatggcaaac aacctctttc
    60901 agctctacca gcgacatgcc aattgggtta gggcggaaag tagagcgtgt tgcgaaaacc
    60961 cccattctgg cgttaccgcc gaggcgcggt ggacgcacag tcggacgcca gccgccttcc
    61021 atcgtttgat gaaagacgaa aaggatccat aaatggctga atgcttccag gccgcgaacg
    61081 gcgtcggcct ggttgtagga agctatgaga tgcagttcgc cgttggcgct ttttaccaga
    61141 cctggctggc gcggaacggc gaatttttct ttatagggcg agcgaataac gcctatttgc
    61201 tcgaactgga aactgctcat ttcgccgtaa tgttaagcgc agagccgata catacagcct
    61261 gacgatagca gcccggcgta ccgctggtga cttcgcagct atgcagtaat acagcattgg
    61321 ctttcatttt agaggcattg atttgcatct gcttacgtgc ggttggaatg ctcggcggag
    61381 agtcctgatt agaggcctgg caagagtcgc cactgacttc accgagatcg cggaacggtt
    61441 tgccgactaa ttcttctgca ttggtataaa ttcggaccgg cgtggcgcgc ggcgctttcg
    61501 gttttgcagg ctccgctttc ggctggggtg cagtgctttg aacgggttcg acaggggatc
    61561 tgcttaacat ggaacagccg cttagcatga gtgctactaa acagatcggt aaagcacgca
    61621 tagtatttcc tcaatgtatg atcaaaacgt caatattgaa tcaggagctt gtaaaaatga
    61681 caagacgggc aagcgcccgt cctgaatgat attacaaatt gtggaaacag cctaaaaatt
    61741 accagccttt aacagcgccg ccgttaaaca ctttgtttgc tgcttcgtaa acttcatcag
    61801 actgataagc ctggacgaat ttcttcacgt tctcggcgtc tttgttatct tcacgcgtta
    61861 cgatcaggtt tacgtatggg gagtctttat cttcgacaaa gataccgtct ttcgccggag
    61921 tcaggccaat ctggctggca taggtggtat tgataactgc cagagcgatt tgcgcgtcgt
    61981 ccagagaacg cggcagttgc ggtgcttcca gttcaacaat tttcagattt tttgggttct
    62041 caacaacatc aagaacggtc ggcagcaggc caacgccatc tttcagtttg atcaagccca
    62101 ctttttgcag cagcagcagt gaacgaccaa gattagttgg atcgtttggc acggcaacct
    62161 gcgaaccatc ctgcagttca tccagtgatt tgattttctt ggagtaacca gcaatcggat
    62221 aaacaaaagt gttgcctact gcgaccagtt tgtagccacg atctttcagt tgctgatcaa
    62281 ggtacggttt atgctggaag gcgttggcgt cgatatcgcc tttgctcaat gcttcgtttg
    62341 gcagaacata gtcgttgaag gttaccagct caacgtccag gccatatttg tctttcgcaa
    62401 ctttctgcgc gacttctgct acctgctgtt cggcaccaac aatcacgccg actttaatgt
    62461 ggtttggatc tttttcatcc tgaccgcagc ctaccagtgc cagtgatcca atcagggctc
    62521 ccactgccgc aaaggttttg aatttgaacg ccatacctta ttccttcttc aattatttat
    62581 gttgtgttga acgttacttg cgagtgacag cccggacgat gcggtcgcct gcaaactgaa
    62641 ttaaataaac cagaatgacc agcaatacca gtaccgtatt catcaccgtc gcgttgtagc
    62701 cgatgtagcc atactgatag ccaatctgac ctaaaccacc ggcaccgact gcaccaccca
    62761 tcgcggaata accgaccagg gtaatcaggg tgatagttgc cgcattcacc agacccggca
    62821 acgcttccgg taacagcact ttacagacga tctgcattgg cgtggcaccc attgcgcggg
    62881 aagcttcaat taacccggtt gggatctcca gcagagcgtt ctcgaccata cgggcaataa
    62941 acggtgctgc accaacggtt aacggaacaa ttgctgcctg caaaccaatc gatgtaccga
    63001 caataacgcg ggtaaacgga atcatccata ccagcaagat aatgaacggg atggaacgga
    63061 aaatgttcac aatcgcagaa acggtacgat acagcttcgc gttagcaata atttgccccg
    63121 gacgcgtgac ataaagcaga acgccaaccg gcagaccaat cacaaaccca aaaaatccgg
    63181 atacgaaggt cattgccagc gtttcccata cgccacgaac cagcagccac atcatcggct
    63241 cagacataac ccagtacctc tacttttaca tggtgttcct gcagccaggc aatggcggct
    63301 tgcgtatctt gttgtgtgcc gtgcatttca gtcagcatga tgccgaattt aacgccaccg
    63361 gcgtagtcca tctgcgcgct aataatgttg ttgttgacgt tgaaacgacg cgcggtttca
    63421 gaaagcagtg gggcatcgac cgattgaccg gtaaactcca gacgcagcat cggcacgcag
    63481 tcagtaaatg gctccgtttg cagacgttcc tggtaatctt ccgggatatc cagatgcagg
    63541 gtcgactgaa taaacttctg cgccagcggc gttttcggat gcgagaacac ttcacttacc
    63601 gtgtcctgct cgatcagttc gccattgctg atgaccgcca cgcaatcaca aatacgcttc
    63661 acaacgtcca tttcgtgggt gatcaacaga atcgtcaaac ccagacggcg gttaatgtct
    63721 ttcagcagtt cgagaataga acgtgtcgtt gccgggtcca gcgcgctggt ggcttcatca
    63781 cacagcaata ctttgggatt gctggctaat gcacgggcaa tcgccacacg ttgtttctgc
    63841 ccaccggaaa gattcgacgg gtagctatca tgcttatcgc caagaccaac caatgacagc
    63901 aattccgtca cgcgacgttt gatctcgtct ttcggtgtgt tgtccagctc cagcggcaga
    63961 gccacgttgc caaaaacagt acgcgaagag agcaggttaa aatgctggaa aatcatacca
    64021 atctggcggc gagctttggt caactcggat tctgacagcg tggtcaattc ctggccatcg
    64081 accagcacgc taccctcggt tgggcgctcc agcaagttta cacaacgtat aagcgtactc
    64141 ttacccgcgc ctgaggcacc gataacgcca taaatttgtc cagctggcac atgcaggctg
    64201 acgttgttca acgcctggat ggtgcgggtg ccctggtgga acactttggt gatattcgaa
    64261 agttttatca ttgattattt attatcgtca ttaagttagt cgtagcatct cgaatgcctg
    64321 aaacgggcaa cgccgtcaat gaaatggatg ttaaggcatc cagacgtcta aatcaatcag
    64381 gtttatgcga agagcacttt cttgcaggtc gaaacatgcg atactagcgt cccatgcctt
    64441 attaaggagc tataaaaggt ggcgaagagc gtacccgcaa tttttcttga ccgtgatggc
    64501 accattaatg tcgatcacgg ctatgtccat gagatcgaca actttgaatt tatcgacggt
    64561 gttattgacg ccatgcgcga gctaaaaaaa atgggctttg cgctggtggt agtaaccaac
    64621 cagtctggca ttgctcgcgg taaatttacc gaagcacagt ttgaaacgct gaccgagtgg
    64681 atggactggt cgctggcgga ccgagatgtc gatctggatg gtatctatta ttgcccgcat
    64741 catccgcagg ggagtgttga agagtttcgc caggtctgcg attgccgcaa accacatccg
    64801 gggatgcttt tgtcagcacg cgattatttg catattgata tggccgcttc ttatatggtg
    64861 ggcgataaat tagaagatat gcaggcagcg gttgcggcga acgtgggaac aaaagtgctg
    64921 gtgcgtacgg gtaaacctat tacacctgaa gcagaaaacg cggcagattg ggtgttaaat
    64981 agcctggcag acctgccgca agcgataaaa aagcagcaaa aaccggcgca atgattaaaa
    65041 gatgaacggt tgaaataaaa atgcattttt ccgcttgtct tcctgagccg actccctata
    65101 atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca caaacagccg gttcggttga
    65161 agagaaaaaa tcctgaaatt cagggttgac tctgaaatag gaaagtgtga tatacgccac
    65221 ctcgcgacag tgagctgaaa gctgcgtcgc aactgctctt taacaattta tcagacaatc
    65281 tgtgtgggca ctcgaagata cggattctta acgtcgcaag acgataaatg aataccaagt
    65341 ctcaagagtg aacacgtaat tcattacgaa gtttaattct ttgagcatca aacttttaaa
    65401 ttgaagagtt tgatcatggc tcagattgaa cgctggcggc aggcctaaca catgcaagtc
    65461 gaacggtaac aggaagcgct tgctgcttcg ctgacgagtg gcggacgggt gagtaatgtc
    65521 tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata ccgcatgacg
    65581 tcgcaagacc aaagaggggg accttcgggc ctcttgccat cggatgtgcc cagatgggat
    65641 tagcttgttg gtggggtaac ggctcacc
//
LOCUS       DAJTYM010000007        65175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTYM010000007 DAJTYM010000000
VERSION     DAJTYM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..708)
                     /gene="cra"
                     /locus_tag="OO575_000469"
     CDS             complement(<1..708)
                     /gene="cra"
                     /locus_tag="OO575_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6663560.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETH"
     gene            complement(790..876)
                     /locus_tag="OO575_000470"
     CDS             complement(790..876)
                     /locus_tag="OO575_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6663561.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(888..1379)
                     /gene="ilvN"
                     /locus_tag="OO575_000471"
     CDS             complement(888..1379)
                     /gene="ilvN"
                     /locus_tag="OO575_000471"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6663562.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1382..3106)
                     /gene="ilvI"
                     /locus_tag="OO575_000472"
     CDS             complement(1382..3106)
                     /gene="ilvI"
                     /locus_tag="OO575_000472"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6663563.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3225..3269
                     /locus_tag="OO575_000473"
     CDS             3225..3269
                     /locus_tag="OO575_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663564.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3424..4368)
                     /gene="leuO"
                     /locus_tag="OO575_000474"
     CDS             complement(3424..4368)
                     /gene="leuO"
                     /locus_tag="OO575_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6663565.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5027..5113
                     /gene="leuL"
                     /locus_tag="OO575_000475"
     CDS             5027..5113
                     /gene="leuL"
                     /locus_tag="OO575_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6663566.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5206..6777
                     /gene="leuA"
                     /locus_tag="OO575_000476"
     CDS             5206..6777
                     /gene="leuA"
                     /locus_tag="OO575_000476"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6663567.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            6777..7868
                     /gene="leuB"
                     /locus_tag="OO575_000477"
     CDS             6777..7868
                     /gene="leuB"
                     /locus_tag="OO575_000477"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6663568.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            7871..9271
                     /gene="leuC"
                     /locus_tag="OO575_000478"
     CDS             7871..9271
                     /gene="leuC"
                     /locus_tag="OO575_000478"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6663569.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9282..9887
                     /gene="leuD"
                     /locus_tag="OO575_000479"
     CDS             9282..9887
                     /gene="leuD"
                     /locus_tag="OO575_000479"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6663570.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(9936..11114)
                     /gene="setA"
                     /locus_tag="OO575_000480"
     CDS             complement(9936..11114)
                     /gene="setA"
                     /locus_tag="OO575_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6663571.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11216..11347)
                     /gene="sgrT"
                     /locus_tag="OO575_000481"
     CDS             complement(11216..11347)
                     /gene="sgrT"
                     /locus_tag="OO575_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6663572.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11436..13091
                     /gene="sgrR"
                     /locus_tag="OO575_000482"
     CDS             11436..13091
                     /gene="sgrR"
                     /locus_tag="OO575_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6663573.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13125..13224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13255..14238
                     /gene="thiB"
                     /locus_tag="OO575_000483"
     CDS             13255..14238
                     /gene="thiB"
                     /locus_tag="OO575_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6663574.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14214..15824
                     /gene="thiP"
                     /locus_tag="OO575_000484"
     CDS             14214..15824
                     /gene="thiP"
                     /locus_tag="OO575_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6663575.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            15808..16506
                     /gene="thiQ"
                     /locus_tag="OO575_000485"
     CDS             15808..16506
                     /gene="thiQ"
                     /locus_tag="OO575_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6663576.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16620..17384)
                     /locus_tag="OO575_000486"
     CDS             complement(16620..17384)
                     /locus_tag="OO575_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6663577.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17470..18348)
                     /gene="araC"
                     /locus_tag="OO575_000487"
     CDS             complement(17470..18348)
                     /gene="araC"
                     /locus_tag="OO575_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6663578.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            18687..20387
                     /gene="araB"
                     /locus_tag="OO575_000488"
     CDS             18687..20387
                     /gene="araB"
                     /locus_tag="OO575_000488"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6663579.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20398..21900
                     /gene="araA"
                     /locus_tag="OO575_000489"
     CDS             20398..21900
                     /gene="araA"
                     /locus_tag="OO575_000489"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6663580.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22100..22795
                     /gene="araD"
                     /locus_tag="OO575_000490"
     CDS             22100..22795
                     /gene="araD"
                     /locus_tag="OO575_000490"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6663581.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            22870..25221
                     /gene="polB"
                     /locus_tag="OO575_000491"
     CDS             22870..25221
                     /gene="polB"
                     /locus_tag="OO575_000491"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6663582.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25386..28292
                     /gene="rapA"
                     /locus_tag="OO575_000492"
     CDS             25386..28292
                     /gene="rapA"
                     /locus_tag="OO575_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6663583.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28304..28963
                     /gene="rluA"
                     /locus_tag="OO575_000493"
     CDS             28304..28963
                     /gene="rluA"
                     /locus_tag="OO575_000493"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6663584.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29080..29895)
                     /gene="djlA"
                     /locus_tag="OO575_000494"
     CDS             complement(29080..29895)
                     /gene="djlA"
                     /locus_tag="OO575_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6663585.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30150..32504
                     /gene="lptD"
                     /locus_tag="OO575_000495"
     CDS             30150..32504
                     /gene="lptD"
                     /locus_tag="OO575_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6663586.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32557..33843
                     /gene="surA"
                     /locus_tag="OO575_000496"
     CDS             32557..33843
                     /gene="surA"
                     /locus_tag="OO575_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6663587.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            33843..34832
                     /gene="pdxA"
                     /locus_tag="OO575_000497"
     CDS             33843..34832
                     /gene="pdxA"
                     /locus_tag="OO575_000497"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6663588.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            34829..35650
                     /gene="rsmA"
                     /locus_tag="OO575_000498"
     CDS             34829..35650
                     /gene="rsmA"
                     /locus_tag="OO575_000498"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6663589.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            35653..36030
                     /gene="apaG"
                     /locus_tag="OO575_000499"
     CDS             35653..36030
                     /gene="apaG"
                     /locus_tag="OO575_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6663590.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36037..36879
                     /gene="apaH"
                     /locus_tag="OO575_000500"
     CDS             36037..36879
                     /gene="apaH"
                     /locus_tag="OO575_000500"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6663591.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(36957..37436)
                     /gene="folA"
                     /locus_tag="OO575_000501"
     CDS             complement(36957..37436)
                     /gene="folA"
                     /locus_tag="OO575_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6663592.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37627..39489)
                     /gene="kefC"
                     /locus_tag="OO575_000502"
     CDS             complement(37627..39489)
                     /gene="kefC"
                     /locus_tag="OO575_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6663593.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39482..40012)
                     /gene="kefF"
                     /locus_tag="OO575_000503"
     CDS             complement(39482..40012)
                     /gene="kefF"
                     /locus_tag="OO575_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6663594.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40060..41454)
                     /locus_tag="OO575_000504"
     CDS             complement(40060..41454)
                     /locus_tag="OO575_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6663595.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41513..41800)
                     /gene="fixX"
                     /locus_tag="OO575_000505"
     CDS             complement(41513..41800)
                     /gene="fixX"
                     /locus_tag="OO575_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6663596.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(41797..43083)
                     /locus_tag="OO575_000506"
     CDS             complement(41797..43083)
                     /locus_tag="OO575_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6663597.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43134..44075)
                     /locus_tag="OO575_000507"
                     /pseudo
     CDS             complement(43134..44075)
                     /locus_tag="OO575_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44090..44860)
                     /locus_tag="OO575_000508"
     CDS             complement(44090..44860)
                     /locus_tag="OO575_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6663598.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45216..46730
                     /gene="caiT"
                     /locus_tag="OO575_000509"
     CDS             45216..46730
                     /gene="caiT"
                     /locus_tag="OO575_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6663599.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            46761..47903
                     /gene="caiA"
                     /locus_tag="OO575_000510"
     CDS             46761..47903
                     /gene="caiA"
                     /locus_tag="OO575_000510"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6663600.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48032..49249
                     /gene="caiB"
                     /locus_tag="OO575_000511"
     CDS             48032..49249
                     /gene="caiB"
                     /locus_tag="OO575_000511"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6663601.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49323..50876
                     /gene="caiC"
                     /locus_tag="OO575_000512"
     CDS             49323..50876
                     /gene="caiC"
                     /locus_tag="OO575_000512"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6663602.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            50985..51770
                     /gene="caiD"
                     /locus_tag="OO575_000513"
     CDS             50985..51770
                     /gene="caiD"
                     /locus_tag="OO575_000513"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6663603.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            51776..52366
                     /gene="caiE"
                     /locus_tag="OO575_000514"
     CDS             51776..52366
                     /gene="caiE"
                     /locus_tag="OO575_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6663604.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52452..52847)
                     /gene="caiF"
                     /locus_tag="OO575_000515"
     CDS             complement(52452..52847)
                     /gene="caiF"
                     /locus_tag="OO575_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6663605.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53109..56330)
                     /gene="carB"
                     /locus_tag="OO575_000516"
     CDS             complement(53109..56330)
                     /gene="carB"
                     /locus_tag="OO575_000516"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6663606.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56348..57496)
                     /gene="carA"
                     /locus_tag="OO575_000517"
     CDS             complement(56348..57496)
                     /gene="carA"
                     /locus_tag="OO575_000517"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6663607.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(57952..58773)
                     /gene="dapB"
                     /locus_tag="OO575_000518"
     CDS             complement(57952..58773)
                     /gene="dapB"
                     /locus_tag="OO575_000518"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6663608.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(58940..59854)
                     /gene="rihC"
                     /locus_tag="OO575_000519"
     CDS             complement(58940..59854)
                     /gene="rihC"
                     /locus_tag="OO575_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6663609.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(59920..60870)
                     /gene="ispH"
                     /locus_tag="OO575_000520"
                     /gene_synonym="lytB"
     CDS             complement(59920..60870)
                     /gene="ispH"
                     /locus_tag="OO575_000520"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6663610.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(60872..61321)
                     /gene="fkpB"
                     /locus_tag="OO575_000521"
     CDS             complement(60872..61321)
                     /gene="fkpB"
                     /locus_tag="OO575_000521"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6663611.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61408..61902)
                     /gene="lspA"
                     /locus_tag="OO575_000522"
     CDS             complement(61408..61902)
                     /gene="lspA"
                     /locus_tag="OO575_000522"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6663612.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(61902..64718)
                     /gene="ileS"
                     /locus_tag="OO575_000523"
     CDS             complement(61902..64718)
                     /gene="ileS"
                     /locus_tag="OO575_000523"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6663613.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(64761..>65175)
                     /gene="ribF"
                     /locus_tag="OO575_000524"
     CDS             complement(64761..>65175)
                     /gene="ribF"
                     /locus_tag="OO575_000524"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR6663614.1"
                     /translation="NLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQVSP
                     VKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVLRK
                     KIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
BASE COUNT        15745 a        17763 c        16831 g        14836 t
ORIGIN      
        1 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
       61 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      121 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      181 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      241 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      301 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      361 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      421 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      481 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      541 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      601 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
      661 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
      721 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
      781 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
      841 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
      901 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
      961 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1021 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1081 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1141 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1201 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1261 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1321 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1381 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1441 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1501 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1561 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1621 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     1681 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     1741 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     1801 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     1861 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     1921 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     1981 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2041 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2101 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2161 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2221 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2281 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2341 ccagtgcctg acgatgcgtt gccggaaacg cccccattcc catcaatgag gaaacaacgg
     2401 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2461 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2521 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2581 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     2641 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     2701 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     2761 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     2821 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccttggac
     2881 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     2941 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3001 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3061 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3121 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3181 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3241 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3301 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3361 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3421 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3481 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3541 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3601 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     3661 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     3721 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     3781 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     3841 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     3901 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     3961 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4021 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4081 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4141 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4201 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatgttttg
     4261 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4321 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4381 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4441 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4501 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4561 aatccataat gttttgttgc gaaaacaatc taatcataac taccgcgaat actcaatcat
     4621 ctacaaaatg gattaaatgt gcttatttaa taattaattt atatatttaa tgcattaatt
     4681 cttaacatta attgttcaat aatattcacc aaatcaatat caaaaaaaat cgcaaaacat
     4741 ataattcaat acaaatcatc aggataggtt ttgcaacgcg tgcattttgt cccctttttc
     4801 ctcgttgatt agatgcaaaa atttatgctg aaatatgtca accgatgaaa agcgtcggta
     4861 gttaagcaga aattaatatc gcttacttta accaccgtag cacaattagc taattttacg
     4921 gatgcagaac tcacgctggc gggacgtttt tattgcgtca gggttgacat ccgtttttgt
     4981 atccagtaac tctaaaagca tatcgcattc atctggagct gatttaatga ctcacatcgt
     5041 tcgctttatc ggtctactac tactaaacgc atcttctttg cgcggtagac gagtgagcgg
     5101 catccagcat taagccagca cgcagtcaaa caaaaaaccc gcgccattgc gcgggttttt
     5161 ttatgcccga agcgaggcgc tctaaaagag acaaggaccc aaaccatgag ccagcaagtc
     5221 attattttcg ataccacatt gcgcgacggt gaacaggcgt tacaggcaag cttgagtgtg
     5281 aaagaaaaac tgcaaattgc gctggccctt gagcgtatgg gtgttgacgt gatggaagtc
     5341 ggtttccccg tctcttcgcc gggcgatttt gaatcggtgc aaaccatcgc ccgccaggtt
     5401 aaaaacagcc gcgtatgtgc gttagctcgc tgcgtggaaa aagatatcga cgtggcggcc
     5461 gaatccctga aagtcgccga agccttccgt attcatacct ttattgctac ctccccaatg
     5521 cacatcgcca ccaagctgcg cagcacccta gacgaggtga tcgaacgcgc tatctatatg
     5581 gtgaaacgcg cccgtaatta caccgatgat gttgaatttt cttgcgaaga tgccgggcgt
     5641 acacccattg ccgatctggc gcgagtggtc gaagcggcga ttaatgccgg tgccaccacc
     5701 atcaacattc cggacaccgt gggctacacc atgccgtttg agttcgccgg aatcatcagc
     5761 ggcctgtatg aacgcgtacc taacatcgac aaagccatta tttccgtaca tacccacgac
     5821 gatttaggcc tggcggtcgg caactcactg gcggcggtac atgccggtgc acgccaggtg
     5881 gaaggtgcaa tgaacgggat cggcgagcgt gccggtaact gttcgctgga agaagtcatc
     5941 atggcgatta aagttcgtaa ggatattctc aacgtccaca ccgccattaa tcaccaagag
     6001 atatggcgca ccagccagtt agttagccag atttgtaata tgccgatccc ggcaaacaaa
     6061 gccattgttg gcagcggcgc attcgctcac tcctccggta tccaccagga tggcgtgctg
     6121 aaaaaccgcg aaaactacga aatcatgaca ccagaatcta ttggtctgaa ccaaatccag
     6181 ctgaatctga cctctcgttc ggggcgtgcg gcggtgaaac atcgcatgga tgagatgggg
     6241 tataaagaaa gtgaatataa tttagacaat ttgtacgatg ctttcctgaa gctggcggac
     6301 aaaaaaggtc aggtgtttga ttacgatctg gaggcgctgg ccttcatcgg taagcagcaa
     6361 gaagagccgg agcatttccg tctggattac ttcagcgtgc agtctggctc taacgatatc
     6421 gccaccgccg ccgtcaaact ggcctgtggc gaagaagtca aagcagaagc cgccaacggt
     6481 aacggtccgg tcgatgccgt ctatcaggca attaaccgca tcactgacta taacgtcgaa
     6541 ctggtgaaat acagcctgac cgccaaaggt cacggtaaag atgcgctggg tcaggtggat
     6601 attgtcacca actacaacgg tcgccgcttc cacggcgtcg gcctggccac cgatattgtc
     6661 gagtcctccg ccaaagccat ggtgcacgta ctgaacaata tctggcgtgc cgcagaagtc
     6721 gaaaaagagt tgcaacgcaa aactcaacac aacgaaaaca acaaggaaac cgtgtgatgt
     6781 cgaagaatta ccatattgcc gtattgccgg gagacggtat tggtccggaa gtgatgaccc
     6841 aggcgctgaa agtgctgaat gccgtgcgca accgctttgc gatgcgcatc accaccagcc
     6901 attacgatgt aggcggcgca gccattgata accacgggca accactgccg cctgcgacgg
     6961 ttgaaggttg tgagcaagcc gatgccgtgc tgtttggctc ggtaggcggc ccgaagtggg
     7021 aacatttacc accagaccag caaccagaac gcggcgcgct gctgccgctg cgtaagcact
     7081 tcaaattatt cagcaacctg cgcccggcaa aactgtatca ggggctggaa gcattctgtc
     7141 cgctgcgtgc agacattgcc gctaacggct tcgacatcca gtgcgtgcgc gaactgaccg
     7201 gcggcatcta tttcggtcag ccaaaaggcc gcgaaggtag cggacaatat gaaaaagcgt
     7261 ttgataccga ggtgtatcac cgttttgaga tcgagcgtat tgcccgcatc gcgtttgaat
     7321 ctgctcgtaa acgtcgccac aaagtcacct caatcgacaa agctaacgtg ctgcaatcct
     7381 ctattttatg gcgtgaaatc gtcaacgaga tcgccacgga atacccggat gtcgaactgg
     7441 cgcatatgta catcgacaac gccaccatgc agctgattaa agatccatca cagtttgacg
     7501 ttctgctgtg ctccaacctg tttggcgaca ttctgtctga cgagtgcgca atgatcactg
     7561 gctcgatggg catgttgcct tccgccagcc tgaacgagca aggttttgga ctgtatgaac
     7621 cggcgggcgg ctcggcacca gatatcgcag gcaaaaacat cgccaacccg attgcacaaa
     7681 tcctgtcgct ggcactgctg ctgcgttaca gcctggatgc cgatgatgcg gcttccgcca
     7741 ttgaacgcgc cattaaccgt gcattagaag aaggcattcg caccggggat ttagcccgtg
     7801 gcgctgccgc cgttagtacc gatgaaatgg gcgatatcat tgcccgctat gtggcagaag
     7861 gggtgtaatc atggctaaaa cgttatacga aaaattattc gacgctcacg tagtgtacga
     7921 agccgaaaac gaaaccccgc tgttatatat cgaccgccac ctggtgcatg aagtgacctc
     7981 accgcaggcg ttcgatggtc tgcgcgccca cggtcgcccg gtacgtcagc cgggcaaaac
     8041 cttcgccact atggatcaca acgtctctac ccagaccaaa gacattaatg cctgtggtga
     8101 aatggcgcgc atccagatgc aggagctgat taaaaactgc aaagaatttg gcgtcgagct
     8161 gtatgacctg aatcacccgt atcaggggat cgtccacgta atggggccgg aacagggcgt
     8221 caccttgccg gggatgacca ttgtctgcgg cgactcgcat accgccaccc acggcgcgtt
     8281 tggcgcactg gcctttggta tcggcacttc cgaagttgaa cacgtactgg caacacaaac
     8341 cctgaaacag ggccgtgcga agaccatgaa aattgaagtc cagggcaaag ccgcgccggg
     8401 cattaccgca aaagatatcg tgctggcaat tatcggtaaa actggcagcg caggcggcac
     8461 cgggcatgtg gtggagtttt gcggcgaagc aattcgtgat ttaagcatgg aaggtcgtat
     8521 gaccctgtgc aatatggcga tcgaaatggg cgcaaaagcc gctctggttg ctcctgacga
     8581 aaccaccttt aactatgtca aaggccgtct gcatgcgccg aaaggcaaag atttcgacga
     8641 cgccgttgcc tactggaaaa ccctgcaaac cgacgaaggc gcaactttcg ataccgttgt
     8701 cactctgcaa gcagaagaaa tttcaccgca ggtcacctgg ggcaccaatc ccggccaggt
     8761 gatttctgtg aacgacaata ttcccgatcc ggcttcgttt gccgatccgg ttgaacgtgc
     8821 gtcggcagaa aaagcgctgg cctatatggg gctgaaaccg ggtattccgc tgaccgaagt
     8881 ggctatcgac aaagtgttta tcggttcctg taccaactct cgcattgaag atttacgcgc
     8941 ggcagcggag atcgccaaag ggcgaaaagt cgcgccaggc gtgcaggcac tggtggttcc
     9001 cggctctggc ccggtaaaag cccaggcgga agcggaaggt ctggataaaa tatttattga
     9061 agccggtttt gaatggcgct tgcctggctg ctcaatgtgt ctggcgatga acaacgaccg
     9121 tctgaatccg ggcgaacgtt gtgcatccac cagcaaccgt aactttgaag gccgccaggg
     9181 gcgcggcggg cgcacgcatc tggtcagccc ggcaatggct gccgctgctg ctgtgaccgg
     9241 acatttcgcc gacattcgca acattaaata aggagcacac catggcagag aaatttatca
     9301 aacacacagg cctggtggtt ccgctggatg ccgccaatgt cgataccgat gcaatcatcc
     9361 cgaaacagtt tttgcagaaa gtgacccgta cgggttttgg cgcgcatctg tttaacgact
     9421 ggcgttttct cgatgaaaaa ggccaacagc caaacccgga cttcgtgctg aacttcccgc
     9481 agtatcaggg cgcttccatt ttgctggcac gagaaaactt cggctgtggc tcctcgcgtg
     9541 agcacgcgcc ctgggcattg accgactacg gttttaaagt ggtgattgcg ccgagttttg
     9601 ctgacatctt ctacggcaat agctttaaca accagctgct gccggtgaaa ttaagcgatg
     9661 cagaagtgga cgaactgttt gcgctggtga aagccaatcc ggggatccat ttcgacgtgg
     9721 atctggaagc gcaagaggtg aaagcgggag agaaaaccta tcgctttacc atcgatgcct
     9781 tccgccgcca ctgcatgatg aatggtctgg acagcatcgg cctgaccctg cagcacgacg
     9841 acgctattgc gtcttatgaa gcaaaacaac ctgcgtttat gaattagacc ccttgcccgg
     9901 tcaaatgacc gggctttccg ctatcgtcca cgtcatcaaa cgtctttaac ctttgcggtt
     9961 aaaaataatg cgacaacaga aataatcgca attacccagt agacagcaaa gtgcccccaa
    10021 ctttgtgcaa ttgctccctg aataacacca gccagaatta ccccggtaga gatagtgttg
    10081 gtaaataagg tggtagctgc ccccgctctt ccaggcatta aatcctgaaa ccatagcatc
    10141 ccaatacccg caacaatgcc gataaataca gcgttaaaaa gttgcagcgt catcaacgcc
    10201 atacggctat gaaagaaaat caatccagtg taaaacagca ctcctgccac cactgcaatg
    10261 accatcattc gccgcttacc ataacgtttg acatagtagc cagccagaat cattgctggt
    10321 atttccagtc cagctgccgt ccccatcagg aaacccgcca gtttgtctgg caatcctaaa
    10381 tcgctactga tccacaatgg catatcaata atgtacatag tgttgcaggt ccacattaac
    10441 gtcgaggcga caaataacat ccgtacgtta ctatcctgcc agtcaccttg cattgataaa
    10501 gcattttccg acggcagttc tacccgcgcc acagacagaa gcataaatgc aatcaatacc
    10561 agactgagtg tgaatatccc ggcggcaatc gaaaacatca ctgtaaagcc gtaattcaac
    10621 gccagcataa aggccagcgg tggaccgatt acccatgcca gagaaagctg cgcacgcatc
    10681 accgagctaa acatcaccac ttctcgcgcc gagttatccg cgtattcccg cgccagagca
    10741 aataactgtg gcattgccgt attggccaga gatgccagaa gcacaccaca ggtgataagc
    10801 gtcagataat gacgattaaa tgcaaacagt agcgcattgc caatagccat caaacagcaa
    10861 aatataatca gttttcgccg atcgccctga ctgtcagaac gttttgccaa ccagaggctt
    10921 acgccgatcc cagcaatagc attcaccgta taaaagagtc cgatccagaa aggttgcgcg
    10981 ccaacctcac gactcagaaa taagctcaat gtaggagcct gtagcgcccc ggccaccccc
    11041 atcataaaag cgaccagcat aaatgccgcg taaacaccgt tcatacggcg agccatcgtc
    11101 attatccaga tcatacgttc cctttttagc gcggcgagaa taaaaaaaac cagcaggtat
    11161 aatctgctgg cgggtgattt tacaccaata ctcagtcaca catgatgcag gcaagtcaac
    11221 tttcagaatt gcggtcatcc cactgcatca gtccttcaag catggttaat cgttgtggga
    11281 cgcttaacca acgcaaccag cacaacttcg ctgtcgcggt aaaatagtgc tgataaaact
    11341 gacgcatggg gcacctcctt gcttcatcgt taggaattat tgacttaata tagggaaaat
    11401 aaaattgctg tcttttgcgc aggagttccc cttttatgcc atctgctcgt ctgcaacaac
    11461 agttcatccg cctgtggcaa tgctgcgagg gtaaatcgca ggacacaacg ctcaacgaac
    11521 tggcagcgtt attgagctgc tcgcgtcgtc atatgcgtac cctgctcaac accatgcagg
    11581 atcgcggctg gctgacgtgg gaagcggaag tcgggcgcgg taaacgctcg cgtctgacat
    11641 tcctctatac cgggctggcg cttcagcaac agcgggcgga agacctgctg gagcaggatc
    11701 gtatcgacca actagtgcag ttggttggcg acaaagcgac tgtgcggcaa atgctggttt
    11761 ctcatctggg ccgcagcttc cgccaggggc ggcacatcct gcgcgtgctc tactatcgtc
    11821 cgttgcgtaa tctgctacct ggcagcgcat tgcgccgttc cgaaacccat attgcccggc
    11881 aaatcttcag ttcgctaacg cgcataaatg aggaaaatgg ggaactggaa gcagacatcg
    11941 cccaccactg gcagcaaata tcaccgcttc actggcgttt ctttttgcgt ccaggagtcc
    12001 attttcatca tggtcgtgaa ctggaaatgg acgacgtgat cgcctcttta aaacgaatca
    12061 atacgctgcc gctctattcg catattacgg acattgtgtc gccgacgccc tggacgctgg
    12121 atatccatct cacgcagccg gaccgctggt tgccgttact gctggggcaa gttccggcga
    12181 tgatcctgcc gtgcgaatgg gaaaccctca gtaactttgc cagccatcct attggcaccg
    12241 gtccgtatgc ggtgattcgc aacagcacca atcaactgaa aattcaggca ttcgatgact
    12301 tcttcggtta ccgggcatta atcgacgaag ttaacgtctg ggttctgccg gaaattgccg
    12361 acgagccagc cggtggtctg atgctaaaag gtccacaggg cgagaaaaaa gagattgaaa
    12421 gccgcctgga ggaaggttgc tactatttac tgttcgacag ccgcacccat cgcggggcga
    12481 atcagcaagt cagggactgg gtaagctatg tgctttctcc aactaatctg gtctatttcg
    12541 ctgaggaaca gtaccagcaa ctgtggttcc cggcttatgg actgctcccc cgttggcacc
    12601 atgcccgcac cataaagagc gaaaaaccgg ctggcctgga aagcctcacc ctgacctttt
    12661 atcaggatca cagtgagcat cgggtgattg ccgggatcat gcagcagatt ctggcaagtc
    12721 accaggtcac gctggaaatc aaagagatca gctacgatca gtggcatgaa ggagagatcg
    12781 agagcgatat ctggcttaat agcgccaact ttacgctgcc gctggatttt tcgctgttcg
    12841 cgcacttgtg cgaggtgccg ctgctccaac actgtattcc gatcgactgg caagccgacg
    12901 ccgcccgctg gcgcaatggc gaaatgaacc tggcgaactg gtgccagcaa ctggtcgcca
    12961 gtaaagcaat ggtgccgctt atccaccact ggctgatcat tcagggacaa cgcagtatgc
    13021 gcggcctgcg tatgaatacc ctcggctggt tcgattttaa atcagcgtgg tttgcgccac
    13081 cggatccatg attgctggta gataacaaaa tcactacact aacgccgttc tcaacggggt
    13141 gccacgcaaa cgcgtgcgct gagaaaatac ccgtcgaacc tgatccggat aacgccggcg
    13201 aagggatttg aggctccttc tcaagtcctt tgccactctt ttttgaggtg caaagtgtta
    13261 aaaaaatgtc ttcccctgct gttgctgtgc acagcgcccg ttttcgctaa acccgttctg
    13321 actgtttata cctacgattc cttcgccgcc gactgggggc ctggtccaaa gatcaaaaaa
    13381 gcttttgaag ccaactgtaa ctgcgaactg aaactggtgg cgctggaaga tggcgtttcg
    13441 cttctcaacc gtctacggat ggaaggcaaa aatagtaaag ccgatgtggt gctggggctg
    13501 gataacaatc tgttagacgc cgccagtaaa accggactat ttgccaaaag cggtgtggca
    13561 gcggatgccg ttaacgttcc cggcggctgg aataatgaca ctttcgtccc gtttgattac
    13621 ggctatttcg ccttcgtcta tgacaagaac aaactgaaaa acccgccgca aagcctgaaa
    13681 gaattggttg agagcgatca gaactggcgg gtgatttatc aggatccgcg taccagcacg
    13741 ccggggctgg gtctgctgtt gtggatgcaa aaagtctatg gcgatgacgc cccacaagcc
    13801 tggcaaaaac tggcgaagaa aaccgtcacg gtcaccaaag gctggagcga agcctacggc
    13861 ctgtttttaa aaggtgaaag cgatctggta ctgagttaca ccacctctcc ggcttatcac
    13921 attctcgaag agaaaaaaga taactacgcc gccgcgaact ttagcgaagg tcactatctg
    13981 caggtggaag tcgccgcccg caccgctgcc agcaagcagc cggagctggc gcaaaaattc
    14041 ctccagttta tggtttctcc ggcttttcag aatgcgatcc caaccggtaa ctggatgtat
    14101 ccggtggcaa atgtcacgct gcctgccggg tttgaacaat tgaccaaacc ggccaccacg
    14161 ctggagttca caccagctga agtggcggca caacgtcagg catggattag cgaatggcaa
    14221 cgcgccgtca gccgttaatt cccggctggt taattccagg tgtaagcgcc gccacgctgg
    14281 tggtagcggt tgcgctcgcg gcgtttctcg ccctgtggtg gaacgcgccg cagggtaact
    14341 gggtggcagt ctggcaggac agctacctgt ggcatgtggt gcgcttctcc ttctggcagg
    14401 cgtttctctc ggcgctactc tctgtcgtac ccgcgatatt cctcgcccgc gccctctatc
    14461 gcaggcgctt tccgggtcgg ctggcgctgt tgcgtctgtg cgcaatgacc ttgatcctcc
    14521 cggtgctagt tgctgttttc ggcattctta gcgtctatgg tcgtcagggc tggctggcat
    14581 cgctctgcca atcgctcggt ctggagtgga ccttttcgcc ctacggcctg caaggcattt
    14641 tgctggcgca cgtatttttt aatctgccga tggcgagccg cttattactc caggcactgg
    14701 aaaacattcc cggcgaacaa cgtcagcttg ccgcccagct tgggatgcgc ggctggcatt
    14761 ttttccgctt cgtcgaatgg ccgtggctac ggcgacaaat cccgccggtt gccgcgctta
    14821 tctttatgct ctgtttcgcc agcttcgcca ccgtgctatc gctgggcggc ggtccgcagg
    14881 caaccactat cgagctggct atttatcagg cgctgagtta cgactacgat cctgcccgcg
    14941 cggcgatgct ggcgctgctc cagatggtgt gttgccttgg gctggtgctg ttgagtcagc
    15001 gactaagtaa ggccattgcg cccggcacca cgctgctgca aggctggcgc gatccggacg
    15061 atcgtctgca tagccgcatt tgcgacacgg tgttaatagt gctggcgctg ctgctgttgc
    15121 tgccaccgtt gctggcggtg atcgtcgatg gggtaaatcg ccagttgccg gaagtgctgg
    15181 cacaaccggt gctgtggcag gcgctgtgga cctcgttgcg tattgcgctg gcggcaggtg
    15241 tattgtgcgt agtgctgacc atgatgctgc tatggagcag tcgcgaactg cgggcgcggc
    15301 agaaaatgct ggcgggtcag gcgctggaga tgagcggcat gttgatcctc gccatgccgg
    15361 ggattgtgct ggctaccggc ttctttttac tgctcaacaa cactatcggc ctgccacaat
    15421 ctgctgacgg cattgtgatt ttcaccaatg cgttaatggc gatcccttat gcgctgaaag
    15481 tactggaaaa cccgatgcgc gatatcaccg cccgctacag tatgttgtgt cagtcgctgg
    15541 gcattgaagg ctggtcgcgc ttaaaagtgg tcgagctgcg cgccctgaaa cgtccactgg
    15601 cgcaggcgct ggcttttgcc tgcgtgctgt cgattggtga ttttggcgta gtggcgttgt
    15661 tcggtaacga tgatttccgt accctgccgt tttatctcta ccagcaaatt ggctcctatc
    15721 gcagccagga cggcgcggtc accgcgttaa ttctgctact actctgtttt ctgctgttta
    15781 ccgtgattga aaaactaccg gggcgaaatg ttaaaactga ctgatatcac ctggctttat
    15841 caccatttgc cgatgcgttt tagcttaacg gtagaacgcg gcgagcaggt ggcaatcctc
    15901 gggccaagcg gcgcgggtaa aagtaccctg ctgaatttga ttgccggttt tctgacgcca
    15961 gccagcggtt cgctgactat cgatggtgtt gatcacacca cgacgccgcc gtcacgccgt
    16021 ccggtgtcga tgctgtttca ggagaacaac ctgttcagcc atctgacggt cgcgcagaac
    16081 atcgggctgg ggctaaatcc ggggttgaaa ctgaacgcgg cacagcagga gaaaatgcac
    16141 gcgattgccc gtcagatggg gattgataat ttaatggcgc ggttaccggg cgagctttcc
    16201 ggcggtcagc gacagcgcgt ggcgttagcg cgttgtctgg tgcgcgaaca gccgatttta
    16261 ttgctcgatg aaccgttctc tgcgctcgat ccggcgttac gtcaggagat gctgacgctg
    16321 gtgagcacga gctgccagca gcaaaaaatg acgctattga tggtgtcgca cagtgtggaa
    16381 gatgcggcgc ggatcgccac acgctcggtg gtggtcgccg acgggcgcat cgcctggcag
    16441 ggtaagactg aagaattgtt gagcggtaag gcgagtgctt cggcaatatt ggggattacg
    16501 ggttagtatg cactgtcaga tgcggcgtga acaccttatc cgacctacgg acagtgcgtt
    16561 ttgtaggcct gataagacgc ggtcagcgtc gcatgaggca acccgcacaa gacaatcccc
    16621 taaaccccaa ccactttacg caaaatatcg atatacaccg gcatcaacgg gtggcgaatt
    16681 aacaccacca gcgccaccac gccgatggca gaaatcaacg gcgtcagcca caacaaacgg
    16741 ccgcgcgaca aataatgact taaacgatcg gttgctttac cgctgcgcca taaccgccag
    16801 cacagccagc caccaaccca caaaaatacc gccgtcgcca gcagcaacca tttaaactcg
    16861 ccgctctgca ttccggctgg aatatcgatc gccgcgcccg ccagaatccc aggcaggaag
    16921 taaaacggtg gccacagcag gcagccgata atattcggcg taatgaattt tgccaccggc
    16981 agatccagca ttcccgccac cattggcacc agcggacgcg tcgggccaac aaagcgaccg
    17041 accagaatgg tgaacatgct gtgttgatgc aacgcatgtt cagttttatc gagtaatgct
    17101 ttgtttttct tcagaaatga ccagcgatgc aacggctttt taaaacgcca gcccagccag
    17161 aaggagatcc agtcgcccag cagacaaccg acaatccctg ccagccaagc gtgccagaaa
    17221 cttaactcgc cgctgccaat cagcgctccc agccccgcca tcagcaccgt accgggtaga
    17281 atcaaaccga ccagcgccag cgactccaga aaggccacca acaccaccgc catcaatgaa
    17341 tacacggtag attgggtaat aaagtgttcc agcaatgctt gcataatgtg cctgtcaaat
    17401 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt
    17461 cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
    17521 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
    17581 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
    17641 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
    17701 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
    17761 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacacgatt
    17821 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
    17881 aagcagattc atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
    17941 ttgcccaaac aggtcgctga aatacggctg gtgcgcttca tccgggcgaa agaaacccgt
    18001 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
    18061 aacccactgg tgataccatt cgcgagcctc cggatgacaa ccgtagtgat gaatctctcc
    18121 tggcgggaac agcaaaatat cacccggtcg gcagacaaat tctcgtccct gatttttcac
    18181 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
    18241 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
    18301 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
    18361 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcgctgcgt
    18421 cttttactgg ctcttctcgc taacccaacc ggtaaccccg cttattaaaa gcattctgta
    18481 acaaagcagg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
    18541 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
    18601 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
    18661 cccgtttttt tggatggagt gaaacgatgg cgattgcaat tggcctcgat tttggcagtg
    18721 attctgtgcg agctttggcg gtggactgcg ccagcggtga agagatcgcc accagcgtag
    18781 agtggtatcc ccgttggcaa aaagggcaat tttgtgatgc cccgaataac cagttccgtc
    18841 atcatccgcg tgactacatt gagtcaatgg aagcggcact gaaaaccgtg cttgcagagc
    18901 ttagcgtcga acagcgcgca gctgtggtcg ggattggcgt tgacagtacc ggctcgacgc
    18961 ccgcaccgat tgatgccgac ggtaacgtgc tggcgctgcg cccggagttt gccgaaaacc
    19021 cgaacgcgat gttcgtattg tggaaagacc acactgcggt tgaagaagcg gaagagatta
    19081 cccgtttgtg ccacgcgccg ggcaacgttg actactcccg ctatattggc ggtatttatt
    19141 ccagcgaatg gttctgggca aaaatcctgc atgtgactcg ccaggacagc gccgtggcgc
    19201 aatctgccgc atcgtggatt gagctgtgcg actgggtgcc agctctgctt tccggtacca
    19261 cccgcccgca ggatattcgt cgcggacgtt gcagcgccgg gcataaatct ctgtggcacg
    19321 aaagctgggg cggcttgccg ccagccagtt tctttgatga gctggacccg atcctcaatc
    19381 gccatttgcc ttccccgctg ttcactgaca cctggactgc cgatattccg gtgggcacct
    19441 tatgcccgga atgggcgcag cgtctcggcc tgcctgaaag cgtggtgatt tccggcggcg
    19501 cgtttgactg ccatatgggc gcagttggcg caggcgcaca gcctaacgca ctggtaaaag
    19561 ttatcggtac ttccacctgc gacattctga ttgccgacaa acagagcgtt ggcgagcggg
    19621 cagttaaagg tatttgcggt caggttgatg gcagcgtggt gcctggattt atcggtctgg
    19681 aagcaggcca atcggcgttt ggtgatatct acgcctggtt tggtcgcgta ctcggctggc
    19741 cgctggaaca gcttgccgcc cagcatccgg aactgaaagc gcaaatcaac gccagccaga
    19801 aacaactgct tccggcgctg accgaagcat gggccaaaaa tccgtctctg gatcacctgc
    19861 cggtggtgct cgactggttt aacggtcgtc gcacgccaaa cgctaaccaa cgcctgaaag
    19921 gggtgattac cgatcttaac ctcgctaccg acactccgct gctgttcggc ggtttgattg
    19981 ctgccaccgc ctttggcgca cgcgcaatca tggagtgctt taccgatcag gggatcgccg
    20041 tcaataacgt gatggcgctg ggcggcatcg cgcggaaaaa ccaggtcatt atgcaggcct
    20101 gctgcgacgt gctgaatcgc ccgctgcaaa ttgttgcctc tgaccagtgc tgtgcgctcg
    20161 gtgcggcgat ttttgctgcc gtcgccgcga aagtgcacgc agacatccca tcagcccagc
    20221 aaaaaatggc cagtgcggta gagaaaaccc tgcaaccgcg cagcgaacag gcacaacgct
    20281 ttgaacagct ttatcgccgc tatcagcaat gggcgatgag cgccgaacaa cactatcttc
    20341 caacttccgc cccggcacag gctgcccagg ccgttgcgac tctataagga cacgataatg
    20401 gcgatttttg ataattatga agtgtggttt gtcattggca gccagcatct gtatggcccg
    20461 gaaaccctgc gtcaggtcac ccaacatgcc gagcacgttg ttaatgcgct gaatacggaa
    20521 gcgaaactgc cctacaaact ggtgttgaaa ccgctgggca ccacgccgga tgaaatcacc
    20581 gctatttgcc gcgacgcgaa ttacgacgat cgttgcgctg gtctggtggt gtggctgcac
    20641 accttctccc cggccaaaat gtggatcaac ggcctgacca tgctcaacaa accgttgctg
    20701 caattccaca cccagttcaa cgcggcgctg ccgtgggaca gtatcgatat ggactttatg
    20761 aacctgaacc agactgcaca tggcggtcgc gagttcggct tcattggcgc gcgtatgcgt
    20821 cagcaacatg ccgtcgttac cggtcactgg caggataaac aagcccatga gcgtatcggc
    20881 tcctggatgc gtcaggcggt ctctaaacag gatacccgtc atctgaaagt ctgccgattt
    20941 ggcgataaca tgcgtgaagt ggcggtcacc gatggcgata aagttgccgc acagatcaag
    21001 ttcggtttct ccgtcaatac ctgggcggtt ggctatctgg tgcaggtggt gaactccatc
    21061 agcgacggcg atgttaacgc gctggtcgat gagtacgaaa gctgctacac catgacgcct
    21121 gccacacaaa tccacggcga aaaacgacag aacgtgctgg aagcggcgcg tattgagctg
    21181 gggatgaagc gtttcctgga acaaggtggc ttccacgcgt tcaccaccac ctttgaagat
    21241 ttgcacggtc tgaaacagct tccaggtctg cccgtacagc gtctgatgca gcagggttac
    21301 ggctttgcgg gcgaaggcga ctggaaaacc gccgccctgc ttcgcatcat gaaggtgatg
    21361 tcaaccggtc tgcagggcgg cacctccttt atggaggact acacctatca cttcgagaaa
    21421 ggtaatgacc tggtgctcgg ctcccatatg ctggaagtct gcccgtcgat tgccgtagaa
    21481 gagaaaccga tcctcgacgt tcagcatctc ggtattggtg gtaaggacga tcctgcccga
    21541 ctgatcttca atacccaaac cggtccagcg attgtcgcca gcctgattga tctcggcgat
    21601 cgttaccgtc tgctggttaa ctgtatcgac acggtgaaaa caccgcactc cctgccgaaa
    21661 ctgccggtgg cgaatgcact gtggaaagcg caaccggatc tgccaactgc ttccgaagcg
    21721 tggatcctcg ctggtggcgc gcaccatacc gtcttcagcc atgcgctgaa cctcaacgat
    21781 atgcgccagt tcgccgagat gcacgacatt gaaatcacgg tgattgataa cgacacccgc
    21841 ctgccagcgt ttaaagacgc actgcgctgg aacgaagtgt attacggatt tcgtcgctaa
    21901 gtagccacat caggtatgta acgcctgatg cgacgctgac gcgtcttatc tggcctacac
    21961 gctgcgattt tgtaggccgg ataagcaaag cgcatccggc attcaacgcc tgatgcgacg
    22021 ctggcgcgtc ttatcaggcc tacgcgctgc gattttgtag accggataag cgaagcgcat
    22081 ccggcacgaa ggagtcaaca tgttaaaaga tctcaaacgc caggtattag aagccaacct
    22141 ggcgctgcca aaacacaacc tggtcacgct cacatggggc aacgtcagcg ccgttgatcg
    22201 cgagcgcggc gtctttgtga tcaaaccttc cggcgtcgat tacagcgtca tgaccgctga
    22261 cgatatggtc gtggttagca tcgaaaccgg tgaagtggtt gaaggtacga aaaagccctc
    22321 ctccgacacg ccaactcacc ggctgctcta tcaggcattc ccctccattg gcggcattgt
    22381 gcatacgcac tcgcgccacg ccaccatctg ggcgcaggcg ggtcagtcga ttccagcaac
    22441 cggcaccacc cacgccgact atttctacgg caccattccc tgcacccgca aaatgaccga
    22501 cgcagaaatc aacggcgaat atgagtggga aaccggtaac gtcatcgtag aaacctttga
    22561 aaaacagggt atcgatgcag cgcaaatgcc cggcgttctg gtccattccc acggcccgtt
    22621 tgcatggggc aaaaatgccg aagatgcggt gcataacgcc atcgtgctgg aagaggtcgc
    22681 ttatatgggg atattctgcc gtcagttagc gccgcagtta ccggatatgc agcaaacgct
    22741 gctggataaa cactatctgc gtaagcatgg cgcgaaggca tattacgggc agtaatgact
    22801 gtataaaacc acagccaatc aaacgaaacc aggctatact caagcctggt tttttgatgg
    22861 attttcagcg tggcgcaggc aggttttatc ttaacccgac actggcggga caccccgcaa
    22921 gggacagaag tctccttctg gctggcgacg gacaacgggc cgttgcaggt tacgcttgca
    22981 ccgcaagagt ccgtggcgtt tattcacgcc gatcaggttc cccgcgctca gcatattttg
    23041 cagggtgaac aaggctttcg tctgacaccg ctggcgttaa aggattttca ccgccagccg
    23101 gtgtatggcc tttactgtcg cgcccatcgc caattgatga attacgaaaa gcgtctgcgt
    23161 gaaggtggcg ttaccgtcta cgaggccgat gtgcgcccgc cagaacgcta tctgatggag
    23221 cggtttatca cctcaccggt gtgggtcgag ggtgatatgc acaatggcac tatcgttaat
    23281 gcccgtctga aaccgcatcc cgactatcgt ccgccgctca agtgggtttc tatagatatt
    23341 gaaaccaccc gccacggtga gctgtactgc atcggcctgg aaggctgcgg gcagcgcatc
    23401 gtttatatgc tggggccgga gaatggcgac gcctcctcgc ttgatttcga actggaatac
    23461 gtcgccagcc gcccgctgct gctggaaaaa ctcaacgcct ggtttgcaaa ccacgatccc
    23521 gatgtgatca tcggctggaa cgtggtgcag ttcgatctgc gaatgctgca aaaacatgcc
    23581 gagcgttacc gtcttccgct gcgtcttggg cgcgataata gcgagctgga gtggcgcgag
    23641 cacggcttta aaaacggcgt cttttttgcc caggctaaag gtcggctaat tatcgacggt
    23701 atcgaggcgc tgaaatccgc gttctggaat ttctcttcat tctcgctgga aactgtcgct
    23761 caggagctat taggcgaagg aaaatctatc gataacccgt gggatcgaat ggacgaaatt
    23821 gaccgccgtt tcgccgaaga taaacctgcg ctggcaactt ataacctgaa agattgcgag
    23881 ctggtgacgc agatcttcca caaaactgaa atcatgccat ttttactcga acgagcaacg
    23941 gtgaacggcc tgccggtgga tcggcacggc ggttcggtgg cagcgtttgg tcatctctat
    24001 tttccgcgaa tgcatcgcgc tggttatgtc gcgcctaatc tcggcgaagt gccgccgcac
    24061 gccagccctg gcggctacgt gatggattca cggccagggc tttatgattc agtgctggtg
    24121 ctggactata aaagcctgta cccgtcgatc atccgcacct ttctgattga tcccgtcggg
    24181 ctggttgaag gcatggcgca acctgatcca gagcacagta ccgaaggttt tctcgatgcc
    24241 tggttctcac gggagaaaca ttgcctgccg gagattgtga ctaacatctg gcacgggcgc
    24301 gataaagcca aacgtcaggg taataagccg ctttcgcagg cgctgaagat cattatgaat
    24361 gccttttatg gcgtgctcgg caccaccgcc tgccgcttct tcgatccgcg cctggcatcg
    24421 tcgatcacca tgcgtggtca tcagatcatg cggcagacca aagcattgat tgaagcgcag
    24481 ggctacgaca ttatctacgg agataccgac tcgacttttg tctggctaaa aggtgcacat
    24541 tcggaagaag aggcggcgaa aatcggtcgt gcactggtgc agcacgttaa cgcctggtgg
    24601 gcggaaacgc tgcaaaaaca acggctgacc agcgcattag aactggagta tgaaactcat
    24661 ttctgccgtt ttcttatgcc aaccattcgc ggagctgata ccggcagtaa aaagcgctac
    24721 gccgggctga ttcaggaggg cgacaagcag cggatggtgt ttaaagggct ggaaaccgtg
    24781 cgcaccgact ggacgccgct ggcccagcag tttcagcagg agctgtacct gcgtatcttc
    24841 cgcaacgagc catatcagga gtacgtccgc caaaccatcg acaaactgat ggcgggtgaa
    24901 ctggatgcgc ggctggttta ccgtaaacgc cttcgccgtc cgctgagcga atatcagcgt
    24961 aatgttccgc ctcatgtacg cgccgctcgc cttgccgatg aagaaaatca aaagcgcggt
    25021 cgccccttgc aatatcagaa tcgcggcacc attaagtacg tatggaccac caacggcccg
    25081 gagccgctgg actaccaacg ttcaccactg gattacgaac actatctgac ccgccagcta
    25141 caacccgtgg cggagggaat actccctttt attgaggata attttgctac acttatgacc
    25201 gggcaacttg ggctattttg agcaaaaaaa agagttcgcc agataccatt ttgatgcgtg
    25261 acgaatgctt tgccatccag taccatagcg ccctttccat tcctggacct gaataacacc
    25321 actacctcat aagcacggta gcgggtggtt attgcctgca attaaagata tagagccgaa
    25381 cacatatgcc ttttacactt ggtcaacgct ggatcagcga tacagaaagc gaattgggac
    25441 ttggaaccgt tgtcgcggtg gatgcgcgaa ctgtcacttt acttttccca tctactggtg
    25501 aaaaccgtct gtacgcacgc agtgattccc ccgtgacccg cgtgatgttc aaccctggtg
    25561 ataccattac cagccatgac ggctggcaga tgcaagtcga agaagtaaaa gaagaaaatg
    25621 gcttgctgac ctatatcggt actcgcctgg atactgaaga gtccggcgta gccctgcgtg
    25681 aagttttcct tgatagcaaa ctggtgttca gcaaaccgca ggaccgtctg tttgccgggc
    25741 agattgaccg tatggaccgc tttgcgctgc gttatcgcgc gcgtaagtat tccagcgaac
    25801 agttccgtat gccgtacagc ggcctgcgcg gtcagcgtac cagcctgatc ccgcatcagc
    25861 tcaacatcgc tcatgatgtt ggtcgccgcc acgcgccgcg cgtcctgctg gctgacgaag
    25921 tgggtttagg gaaaaccatt gaagccggga tgatcctgca tcagcaactg ctctctggcg
    25981 ctgctgaacg tgtgctgatt atcgtcccgg aaaccttaca gcatcagtgg ctggtagaaa
    26041 tgctgcgccg tttcaacctg cgctttgctc tgtttgatga tgagcgttat gccgaagctc
    26101 agcacgatgc ttacaacccg tttgacaccg aacagctggt gatttgctcg ctggattttg
    26161 cccgtcgtag caaacagcgc ttggaacatc tctgtgaagc cgaatgggac ctgctggtgg
    26221 tcgatgaagc gcatcacctg gtgtggagcg aagatgcgcc aagccgtgaa tatcaggcca
    26281 ttgaacaact ggcagagcac gtgccgggcg ttctgctgct gaccgcgacc ccggaacagc
    26341 tggggatgga aagccacttc gcccgtctgc gtctgctgga cccgaaccgt ttccacgatt
    26401 ttgcgcagtt cgttgaagag cagaaaaatt atcgtccggt tgcggacgcc gttgccatgc
    26461 tgctggcagg taacaaactg agcaatgacg aactgaacat gctcggcgag atgatcggcg
    26521 agcaggatat cgagccgctg ttgcaggcag caaacagcga cagcgaagat gcccagagcg
    26581 cccgtcagga gctggtttcg atgctgatgg atcgccacgg caccagccgc gtgctgttcc
    26641 gtaacacgcg taacggtgtg aaaggattcc cgaaacgcga gctgcacacc attaagctgc
    26701 cgctaccgac gcagtatcag acggctatta aagtctccgg cattatgggc gcacgtaaaa
    26761 gtgcggaaga tcgtgctcgc gatatgctct acccggagcg tatttatcag gaatttgaag
    26821 gtgataacgc cacctggtgg aacttcgatc cgcgcgttga gtggctgatg ggctacctga
    26881 ccagccatcg ctctcagaaa gtgctggtga tctgcgccaa agctgccact gcgctgcaac
    26941 tggagcaggt actgcgcgaa cgtgaaggta ttcgcgctgc ggtgttccac gaaggtatgt
    27001 cgattatcga acgtgaccgc gctgccgcct ggtttgccga agaagacacc ggcgcacagg
    27061 tactgctgtg ctcagaaatc ggttctgaag gacgtaactt tcagttcgcc agtcacatgg
    27121 tgatgtttga cctgccattc aacccggatc tactggagca gcgtattggt cgtctggatc
    27181 gtatcggcca ggcgcacgat attcagatcc atgtgcctta tctggagaaa accgctcagt
    27241 cggtgctggt gcgctggtat cacgaaggtc tggatgcatt tgagcacacc tgcccgaccg
    27301 gacgcactat ttacgatagc gtatacaacg atctgattaa ctatctggct tcaccggatc
    27361 aaaccgaagg ctttgacgat ctgatcaaaa gctgccgcga gcaacatgaa gcgctgaaag
    27421 cacagctgga acagggtcgt gaccgcctgc tggaaatcca ctccaacggt ggcgaaaaag
    27481 cccaggcact ggcagaaagc attgaagagc aggatgacga taccaacctg atcgccttcg
    27541 ccatgaacct gttcgatatt atcggtatca atcaggacga tcgcggcgac aacatgatcg
    27601 tgctgacgcc gtccgatcat atgctggtgc cggacttccc tggcctgtcg gaagatggca
    27661 tcaccatcac ctttgatcgt gaagtggcgc tggcgcgtga agatgcacag tttattacct
    27721 gggagcatcc gctgatccgc aacggtctgg atctgatcct ttctggcgat accggtagca
    27781 gcacgatttc actgttaaaa aacaaagcgt tgccggtagg tacgctgttg gtggaactga
    27841 tttatgtggt tgaagcccag gctccgaagc agttgcagct caaccgcttc ctgccaccga
    27901 cgccggtacg tatgctgctg gataaaaacg gcaacaacct ggcggcgcag gtagagtttg
    27961 aaacctttaa ccgccagctt aacgcggtta accgtcacac cggcagcaaa ctggttaacg
    28021 ccgtgcagca ggatgttcac gctatccttc aactgggtga agcgcagatc gagaaatctg
    28081 cccgtgcatt gattgatgca gcgcgtaacg aagccgacga aaaactgtct gccgagctgt
    28141 ctcgtctgga agctctgcgt gcagtgaacc cgaacattcg tgacgacgaa ctgaccgcca
    28201 ttgagagcaa ccgtcagcag gtaatggaaa gcctggatca ggcaggttgg cgtctggatg
    28261 ccctgcgttt gatcgttgta acgcatcagt aacggagccg aaaatgggga tggaaaacta
    28321 caatccaccg caggaaccct ggttggttat cctgtatcag gatgaccata ttatggtggt
    28381 caacaagccg agcggtttgt tgtcagtgcc gggtcgtctg gaagagcaca aagacagcgt
    28441 gatgacgcgc attcagcgtg attatccgca ggcagaatcg gtgcatcgtc tggatatggc
    28501 taccagcggc gtgattgtag tggcgctgac caaagccgcg gagcgggagt taaaacgcca
    28561 gttccgcgag cgcgagccga aaaagcagta tgtggcccgc gtctgggggc atccatcccc
    28621 cgcagaaggt ctggtggatc tgccgctgat ttgcgactgg ccaaaccgcc cgaaacagaa
    28681 agtctgttac gaaacgggta aacctgcgca gacggaatat gaagtggtgg agtatgcggc
    28741 ggataacacg gcaagagtgg tgttaaaacc gattaccggg cgttcgcatc aattgcgtgt
    28801 gcatatgctg gcgctggggc atccgattct cggcgatcgt ttttatgcat caccagaagc
    28861 gagagcgatg gcaccgcgtt tgttgctaca tgcagagatg ttgacgatta cccatccggc
    28921 gtatggcaat agtatgacgt ttaaagcgcc agcggatttt taatcgtcac gctggcgcag
    28981 ttcggtaggc ggataaggcg ttcacgccgc atccggcaat tgtgcgccga ttgcctgatg
    29041 cgacgcgggc cgcgtcttat caggcctaca gtttaaaact tatttaaacc ctttctgctg
    29101 ctttatcagc tcatatgcct gctgaatttc ctgcgctttc tgcttcgcca tctccatcat
    29161 ctcaggcggc aaacctttcg ccaccagctt atcgggatgg tgttcactca tcagcttacg
    29221 gtaggcacgt ttgatggtgg tcgcatcatc cgtcggcttc acgcccagca cattacaggc
    29281 atcttccagc gtcgggccac gctgcgcttg ctgccagtta ccaccgccag attgctgctg
    29341 ataaccgccg ccaaactgtg caccgccctg catcatgcgc aaaaactggt caaactgggc
    29401 gcgggagatc cctaattctt cagcaatgac atacagcacc gcccgttcat tcgggtgcag
    29461 tgaaccatca gcaaacgccg cctgaatctg gatctccaga aacatacgaa ttaagtcaaa
    29521 acgaccaaag cagacactgc gaaactggcg catcttttcg cgcagcgggt aattatctga
    29581 ttttcccacc cggaacgcgt tttgcgccgc agtacgggaa gcgccatgaa ggttcattcg
    29641 gtccatcaac tggctggcga tatgaatatc agcctccgtg acgcgacctt tggatttggt
    29701 taaatgcccc atcacttcaa aagtggtggc aaaaaacagc gcctgacgct cacgctggtt
    29761 ggcgaaccac gccattttac ggctacgggc tttatcaaac atatggccaa ttaacaggcc
    29821 taacactacg ccccaaaagc cgccgcccat cagtaaggcc acggccacgc caatgatttt
    29881 tccccaatac tgcatatatt ccccaaatcg acacacggat atcagggcta tctcccacaa
    29941 tataaaggtg cttttaccgt tttccggctg cggtcaattg tgggacatcg cctataattt
    30001 gcattatcat acctgtcatt caatgccgtg cctaacacca ctgacgctat tcggacagga
    30061 ttaacactag cgtagagatg acgagtacgt tagtctctga ccgtttgtca cgcgcaacgt
    30121 taccgatgat ggaacaataa aatcaacgta tgaaaaaacg tatccccact ctcctggcca
    30181 ccatgattgc caccgccctt tatagtcaac agggactggc agccgacctc gcctcacagt
    30241 gcatgttggg cgtgccaagc tatgaccgtc ctctggtaca gggcgatacc aatgacttac
    30301 ccgtgactat caatgctgac cacgcgaaag gggactaccc ggatgacgcc gtgtttactg
    30361 gcagcgtgga tatcatgcag ggtaacagcc gtctgcaggc cgacgaagtg cagctccatc
    30421 aaaaagaggc accaggacaa ccggagccgg tacgtaccgt tgatgcgctc ggtaatgtcc
    30481 attacgacga taaccaggtg atcctcaaag ggccgaaagg ctgggcgaat ctgaacacca
    30541 aagataccaa cgtctgggaa ggtgattacc agatggtggg tcgccagggt cgcggtaaag
    30601 cggacctgat gaaacaacgt ggcgaaaacc gctataccat tctggataac ggtagcttta
    30661 cctcctgtct gccgggttct gacacctgga gcgtggtagg tagcgaaatt attcatgacc
    30721 gcgaagaaca agttgctgag atctggaacg cccgctttaa ggtgggtccg gtaccgatct
    30781 tttatagccc ctatttgcag ttgccggtgg gtgacaaacg tcgctctggt ttcttgatcc
    30841 cgaacgccaa gtacaccacc accaactact ttgagttcta cctgccatat tactggaaca
    30901 tcgcgccaaa tatggatgcc accatcacgc cgcattatat gcatcgtcgt ggcaacatca
    30961 tgtgggagaa cgaattccgc tacctctccc aggcgggcgc tggcttgatg gaactggact
    31021 atctgccttc agataaagtc tatgaagatg aacacccgaa cgatgacagt tcacgtcgtt
    31081 ggttattcta ctggaaccac tccggggtca tggatcaggt gtggcgtttc aacgtcgact
    31141 acaccaaggt cagcgatcct agctacttca atgatttcga taacaagtac ggttccagta
    31201 ctgacggcta cgcaacgcaa aaattcagcg ttggctatgc ggtgcaaaac ttcaatgcca
    31261 ccgtttcaac caagcagttc caggttttca gcgaacagaa caccagtagc tactcggcag
    31321 agccgcagtt agacgttaat tactaccaga atgatgttgg tccgtttgat acgcgtattt
    31381 acggccaggc agtgcacttt gttaacacca gagacgacat gcctgaagca acccgtgttc
    31441 acctggaacc gaccatcaat ttgccgctct ctaataactg gggcagcatc aataccgaag
    31501 cgaagtttct ggcaacccat tatcagcaaa ccaatcttga ctggtataac tccagaaaca
    31561 cgaccaagct ggacgaatcc gttaaccgcg taatgccgca attcaaagtt gacggcaaaa
    31621 tggtctttga acgcgatatg gaaatgctgg ctccgggtta tacccaaacg ctggaaccgc
    31681 gcgcgcagta tttgtacgtg ccgtatcgcg atcagagcga catctataac tacgactcgt
    31741 ctctgctgca atctgactac tctggcctgt tccgggaccg gacttacggc ggtcttgacc
    31801 gtattgcctc cgctaaccaa gtgacgaccg gtgtcacatc tcgcatatat gatgatgctg
    31861 ccgttgaacg ttttaatatt tccgttggtc aaatctacta tttcacggag tctcgcactg
    31921 gcgatgacaa cataacatgg gagaatgacg acaaaacggg ctcactggtg tgggcaggcg
    31981 acacctactg gcgtatctcc gagcgttggg gattgcgtgg cgggattcag tacgatacac
    32041 gtctggataa cgtagcgacc agtaactcca gcattgaata ccgtcgggat gaagaccgtc
    32101 tggtacagct gaattaccat tacgccagcc cggaatatat tcaggctacg ctgcctaagt
    32161 actattccac tgctgagcaa tataagaatg gtatttcgca ggtaggtgct gtcgccagca
    32221 ggccaattgc cgatcgttgg tccattgttg gggcctacta ctacgacacc aatgcgaaca
    32281 agcaagccga ctctatgtta ggtgtgcaat acagctcctg ctgctatgca attcgcgtcg
    32341 gttacgagcg gaagctgaac ggttgggata acgataaaca acatgcggta tatgacaacg
    32401 caatcggctt taacatcgaa cttcgcggcc tgagctccaa ctacggtctg ggtacgcaag
    32461 agatgctgcg ttcgaacatt ctgccgtatc aaaacacttt gtgatttgtt gatttaccac
    32521 gtaatccgca gtgcggttaa ttgaaatgga aaaagtatga agaactggaa aacgctgctt
    32581 ctcggtatcg ccatgatcgc gaataccagt ttcgctgccc cccaggtagt cgataaagtc
    32641 gcagccgtcg tcaataacgg cgtcgtgctg gaaagcgacg ttgatggatt aatgcagtcg
    32701 gtaaaactga acgctgcgca ggcaaggcag cagcttcctg atgacgcgac gctgcgccac
    32761 caaatcatgg aacgtttgat catggatcaa atcatcctgc agatggggca gaaaatggga
    32821 gtgaaaatct ccgatgagca gctggatcag gcgattgcta acatcgcgaa acagaacaac
    32881 atgacgctgg atcagatgcg cagccgtctg gcttacgatg gtctgaacta caacacttat
    32941 cgtaaccaga tccgcaaaga gatgattatc tctgaagtgc gtaacaacga ggtgcgtcgt
    33001 cgcatcacca tcctgccaca ggaagtcgaa tccctggcgc agcaggtggg taaccaaaac
    33061 gacgccagca ctgagctgaa cctgagccac atcctgatcc cgctgccgga aaacccgacc
    33121 tctgatcagg tgaacgaagc ggaaagccag gcgcgcgcca ttgtcgatca ggcgcgtaac
    33181 ggcgctgatt tcggtaagct ggcgattgct cattctgccg accagcaggc gctgaacggc
    33241 ggccagatgg ggtggggccg tattcaggag ttgccgggga tcttcgccca ggcattaagc
    33301 accgcgaaga aaggcgacat tgttggcccg attcgttccg gcgttggctt ccatattctg
    33361 aaagttaacg acctgcgcgg cgaaagcaaa aatatctcgg tgaccgaagt tcatgctcgc
    33421 catattctgc tgaaaccgtc gccgatcatg actgacgaac aggcccgcgt gaaactggag
    33481 caaattgctg ctgatatcaa gagtggtaaa acgacttttg ctgccgcagc gaaagagttc
    33541 tcgcaggatc caggttctgc taaccagggc ggtgatctcg gctgggctac accagatatt
    33601 ttcgatccag ccttccgtga cgccctgacc cgcctgaaca aaggtcaaat gagtgcaccg
    33661 gttcactctt cattcggctg gcatttaatc gaactgctgg atacccgtaa tgtcgataaa
    33721 accgacgctg cgcagaaaga tcgtgcatac cgcatgctga tgaaccgtaa gttctcggaa
    33781 gaagcagcaa gctggatgca ggaacaacgt gccagcgcct acgttaaaat cctgagcaac
    33841 taatggttaa aacccaacgt gttgtgatca ctcccggcga gcccgccggg attggcccgg
    33901 acttagttgt ccagcttgca cagcgtgagt ggccggtcga actggttgtt tgtgccgatg
    33961 ccactctcct taccgaccgg gcagcgatgc tcggtttgcc gctcaccctc cgcccttatt
    34021 cccccaactc ccctgcacaa ccgcaaacta cgggcacatt aacgctactt cctgtcgcgc
    34081 tacgtgaatc tgtcactgcg gggcagttag cgattgaaaa tgggcattac gtggtggaga
    34141 cgctggcgcg agcgtgtgat ggctgtctga acggtgaatt tgctgcgctg atcacaggcc
    34201 ccgtgcataa aggcgtcatt aacgacgcaa gcattccgtt taccggtcat accgagtttt
    34261 tcgaagagcg ttcgcaggcg aaaaaagtgg tgatgatgct ggcgaccgaa gaacttcgcg
    34321 tggcgctggc aacgacgcat ttaccgctgc gcgatatcgc agatgctatc acccctgcac
    34381 ttttgcacga agtgattgct attttgcatc acgatttgcg tacaaaattt ggtattgccg
    34441 aaccgcgcat tctggtctgc gggctgaatc cgcacgcggg cgaaggcggt catatgggta
    34501 cggaagagat agacaccatt attccggtgc tcgacgagct gcgggcgcag gggatgaaac
    34561 tcaacgggcc gctgcctgcc gataccctgt ttcagccgaa atatctcgat aacgccgacg
    34621 ccgtgctggc gatgtaccac gatcagggtc ttcccgtgct aaaataccag ggcttcgggc
    34681 gcggtgtgaa cattacgctg ggcctgccct ttattcgcac atcagtggac cacggcaccg
    34741 cgcttgaact ggcgggacgt ggcgaagccg atgtcggcag ttttattacg gcgcttaatc
    34801 tcgccatcaa aatgattgtt aacacccaat gaataatcga gtccaccagg gccacttagc
    34861 ccgtaaacgc ttcgggcaaa actttctcaa cgatcagttc gtgatcgaca gcattgtttc
    34921 tgccattaac ccgcaaaagg gccaggcaat ggtcgaaatc ggccccggtc tggcggcatt
    34981 gaccgagccg gtcggcgaac gtctggacca gctgacggtc atcgaacttg accgcgatct
    35041 ggcggcacgt ctgcaaacgc atccattctt aggcccgaaa ctgacgattt atcagcagga
    35101 tgcgatgacc tttaactttg gcgaactggc cgagaaaatg ggtcagccgc tgcgtgtttt
    35161 cggcaacctg ccttataaca tctccacgcc gttgatgttc catctgttta gctatactga
    35221 tgccattgcc gacatgcact ttatgttgca aaaagaggtg gtgaatcgtc tggttgcagg
    35281 accgaacagc aaagcgtatg gtcgattaag cgtcatggcg caatactatt gcaacgtgat
    35341 cccggtactg gaagttccgc cgtcagcctt tacaccacca cccaaagtgg attccgccgt
    35401 cgtgcgcctg gtccctcatg caacgatgcc tcacccggtt aaagatattc gcgtgttgag
    35461 ccgcatcacc accgaagcct ttaaccagcg tcgtaaaacc attcgtaaca gcctcggcaa
    35521 cctgtttagc gtcgaggtgt taaccggaat ggggatcgac ccggcgatgc gagcggaaaa
    35581 tatctctgtc gcgcaatatt gccagatggc gaactatctg gcggagaacg cgcctttgca
    35641 ggagagttaa cgatgatcaa ttcgccccga gtgtgtattc aggttcaaag cgtctacatt
    35701 gaggcacaat cttcacctga taatgaacgt tacgtttttg cttataccgt aaccatacgc
    35761 aatctggggc gagcgccagt gcagttgttg gggcgttact ggctgatcac caatggcaat
    35821 ggccgtgaaa ccgaagtcca gggcgaagga gtggttggcg tccagccact tatcgcgcct
    35881 ggcgaagagt accagtacac cagcggtgca atcattgaaa ccccgctggg caccatgcag
    35941 ggtcactacg aaatgatcga tgaaaatggc gtccctttca gcatcgacat tcccgtattc
    36001 cgactcgccg ttcccacact cattcattaa aagaatatgg cgacatacct tattggcgac
    36061 gttcatggtt gttacgatga actgatcgca ttgctgcata aagtagaatt tacccccggg
    36121 aaagataccc tctggctgac gggcgatctg gtcgcgcgcg gcccgggttc gctggatgtt
    36181 ctgcgctatg tgaaatcctt aggcgacagc gtacgtctgg tgctgggcaa tcacgatctg
    36241 catctgctgg cggtatttgc cgggatcagc cgcaataaac cgaaagatcg cctgacaccg
    36301 ctgctggaag cgccggatgc cgacgagctg cttaactggc tgcgtcgcca gcctctgctg
    36361 caaatcgacg aagagaaaaa gctggtgatg gcccacgcag ggatcacgcc gcagtgggat
    36421 ctgcagaccg ccaaagagtg cgcgcgcgat gtagaagcgg tgctgtcgag tgactcctat
    36481 cccttctttc ttgacgctat gtacggcgat atgccaaata actggtcacc ggaattgcgg
    36541 gggctgggaa gactgcgttt tatcaccaac gcctttaccc gtatgcgttt ttgcttcccg
    36601 aacggtcaac tggatatgta cagcaaagaa tcgccggaag aagcccctgc cccactgaaa
    36661 ccgtggtttg cgattcctgg ccctgtcgct gaagaataca gcatcgcctt tggtcactgg
    36721 gcatcgctgg aaggcaaagg tacgccggaa ggtatttacg cgctggatac cggctgctgc
    36781 tggggtgggt cattaacctg cctgcgctgg gaagataaac agtattttgt ccagccgtcg
    36841 aaccggcata aggatttggg cgaagcggcg gcgtcttaaa cacagcctga tacaggaagg
    36901 ccggataaga cgcgaccggc gtcgcatccg gcactagccg taaattctat acaaacttac
    36961 cgccgctcca gaatctcaaa gcaatagctg tgagagttct gcgcatcggc gtcgtggaat
    37021 tcgctgaata ccgattccca gtcatccggc tcgtaatccg ggaaatgggt gtcgccttcc
    37081 acttctgcgt cgatatgcgt cagatacagt ttttgcgctt ttggcaggaa ctgctcataa
    37141 acgcgaccgc cgccaatcac catgatttct ggtacgtcac cacacgccgc aatggcttca
    37201 tccaccgatt tcacccacgt tacgcgatcg tccgtacccg gttgactgct gaggataata
    37261 tttttgcgtc ctggcaacgg acgaccgatt gattcccaag tatggcggcc cataatcacg
    37321 ggtttattta aggtgttgcg tttaaaccag gcgagatcgg caggcaggtt ccacggcatg
    37381 gcgttttcca tgccgataac gcgatctacc gctaacgccg caatcagact gatcattgag
    37441 atttcccgat aaaaaaattg tcgccactat acgtaaagcg taaaccgtcg tcgactggtg
    37501 cgaggatgat gttgaggaaa attttatatt ctgctggcga gtccacgctc tccccctgga
    37561 ctcgccgcat tacaatgaaa caaaaacaaa cagttagctg taaagtgtga tttacgtcac
    37621 tctttattaa gatgagggtt tcgtttccgg ttcatccgcc atgttgccgg tatgtttacc
    37681 ttcttctgtt ccctgccagc catgtcgttg aattaatgac agatgttcgc ggtcctcggt
    37741 gatgatctca cttaacatcg cgctggtgcg tttatagacc gccgcgcggg ctttggtgtc
    37801 attctcaacc attgccatct cttccaccat ctgaatatta aagcggcgga acacatcggc
    37861 acgttctcgc gcttcatacg gccccagacc taaactttcc agcgccagac gcccggtttt
    37921 cagcgcacct tcaaaggttt cacgctccgg cttttcaacg cctgcctgac gcaaacgaat
    37981 gtagtgatcg acatcgcggg cgcgggcaat aatctgcaaa tgcgggaaat gttctttcac
    38041 catctccgtc agttgcaggt tggtttgcgg atcgtcaata gcgttaatca gcacttccgc
    38101 tttcgccgct ccggcagatt ccagtaaatc catccgcgtg gcatcgccat aaaacacttt
    38161 cataccaaat ttacgcagcg tttcgatatg gtccggatcg tgatcgagca ccaccatttt
    38221 caccccgctg gagagcagta aacgtccggt aatctgtcca aaacgaccga atccggcgat
    38281 aatcacgcgc ggctgttctt catcgatctc atcggcttca cgcgcctcct ccgtagaaga
    38341 ttgctcaagg cgattgagga tcaccagcag aatcggcgtt gctgccatcg acagcgccac
    38401 cgccagggtc agcgatttcg cccactccgg ctccagcaca ttcgccatct gcgccgcacc
    38461 aaataccaca aaggcaaact cactgccctg ccctaacaac accgcaaacc agcgacgctg
    38521 tttatttggc acctgcaacg gtcgggcaat cagccacagc atggcgattt tgatgatgag
    38581 gaaaccgagc agcaaaatga caatgcgcaa tgggttttca atcagcgtgc caaagtctat
    38641 cgacatgcca acaccgatga aaaacagccc caacagcaaa cctttaaacg gttcgatatc
    38701 gctttccaac gcatgacggt attccgagct tgccagcagt acgcccgcca gaaacgcgcc
    38761 catcgccatc gacaagccga cctcttccag cagcaaacca aagccaaaca caaggaataa
    38821 cgccacggca ctaaacactt cccgcaagcc agagcgggca acaaaacgca gcgccggacg
    38881 cgtaacatag cgccccagca ataccaccag taccagcgca cccgccactt ttaacgccga
    38941 gagagcaaat gcgcccatcg tcgtcgaggc actgctcgcc gccagtagcg ggatcatcgc
    39001 caccagcggg attgccgcga tatcctggaa cagcagcact gcaaaggcac tgcggcccat
    39061 ttgcgtcacc atcagattgc gttcattcat cgcctgcatg gcaatcgccg ttgatgagag
    39121 cgccagcgtc ataccgatca attccgcgac ctgccagcgc agcccaagta acatacagaa
    39181 cagccccagc aggccaccgc aaatcaccat ctgcaatgcg ccaccgccga acactgccgc
    39241 acgcagcttc cacagccttt gtggatcgag ttcgaggccg ataataaaca gcatcagcac
    39301 caccccaatc tcggcaaagt gcagaataga ttcggcatcg gtcaccagtc gcagccccca
    39361 cgggccaata atgcagccgg cgatcaggta gccaagtacc gatcccagac caagacgtac
    39421 cgcaatgggt acaatcagcg ccgccgatcc gaggtagatc agcgcctgaa ccagcgtatg
    39481 actatccatg atgggcctcc tgccattcca gcagacgttg cttatagtgg cgcgcctgcc
    39541 cttcgagggt ttcgtcgtca caaataaagg tgcagtgcat ggcaaacggt ggcagccagt
    39601 tcagcccgca gtagattgcc gtcgcctgta gcggctgcga cagcacatca aagcccggat
    39661 gcgcaccaat ttcaaaatgg ctttccccgc cgccggtcgt caccgcccac agcaaatgtt
    39721 tgccatgcag cgccgtgccg ccgtgaccgt aagcccagcc gtgcgacaaa actttatcga
    39781 tccaaagttt aagaagcgga ggaatgctgt accactgcat cgggtgctgc cagacgatca
    39841 gatcggcgcg agagagcgcc tcctgctcgg cggcaatatc gatattgaag tcaggataga
    39901 gttgataaag agagcgaatt tcgacgcctt ccagcgtcct tgcctgttca agcatccgtt
    39961 tattcgcatg ggaatgatgc ggatacggat gcgcataaat gatgaggatc atagttatta
    40021 gcccgcgtgt ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    40081 agtcaataat attgattcag aaaagagaga aatctgaaca aataatccca ttctctgccc
    40141 gcggatcgtc atattgctgg tctgcgccag tgacattcct cgagtctccg gggcaaacgc
    40201 tacggaaatc aacaagccaa acagcgagat acccgccccc attagcatcg tgttactgat
    40261 accgtaatta ttgataaata tcggcagtgc ccaggtcgaa acaatggtgc caatacgact
    40321 taaggacata atcacgccca cggcagaggc gcggatatcc gtcgggaaga gttcattagg
    40381 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    40441 cactaccagc cagatcccca tatccgggat tagccccaaa accgccaacg ccagcgtcat
    40501 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttagcc acagcatcgg
    40561 cggaatacag ccgagcataa agaacaggct aatcaccaca ttccctagtg ccgcgttttt
    40621 gccaaccccc aatcccaacg aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    40681 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    40741 attaaacaga tcgcgaaaac gggtttgctg cggctgctct tcatcgaaag ccaccggttc
    40801 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    40861 gcggggagat tctggcagtt cgaatcgacc aatcaaaatc aacaaacaag ggatcgccgc
    40921 gctacccagc atccagcgcc aaccaccttc cacatcataa agccagtagc cgaccagatc
    40981 ggcgcaggtc gcgccgacat accacatcgc ggcaataaag ctgatggaaa acgcccgctg
    41041 acgggtactg gagaactcgg tgatcatcga agtggcgatg ggataatctg caccgatgac
    41101 aatgccgatg agtacccgca tcaccaacag ttcgacgggg gatgaaacac acatcgtcgc
    41161 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtccgacttt
    41221 atcggagata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    41281 cagcaagcca atccagtcag cgtccagttt cagcgccgga gtcagttgct ccagcgccac
    41341 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    41401 gcggcggtga atagaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    41461 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    41521 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    41581 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    41641 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagcacct
    41701 gtttatcagc atcggccttc acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    41761 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    41821 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    41881 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    41941 ttccgggttg ccgctaaaca tgcgtgggtt atcaaggaac gccggtagtt tctggtacat
    42001 gcgcatatcg cgcagcgggc cactctccag atgctgacga tattccgcga gtttttgctt
    42061 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcagctt ccccggcggc
    42121 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    42181 accggcaatc aatacgccgt caccgaccag ctccggcagc atgttgatgc ctgcttccgg
    42241 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg caacggccgg
    42301 atgctgtttg aaatcttcca gcatttgcgg caccgatttt ttcgcgtcat gcagatgatg
    42361 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    42421 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    42481 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    42541 cacatccgtc ggtttaacgc gttttgccat ccccaacttt tcggcaagga tggagttcac
    42601 cccatcagca aggagcaccg ttttcgcttc aatcacatca ccatcggctt ctacaccgac
    42661 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    42721 cgcttcttcg gcctgctcca tcaaccaggc atcaaattta ctgcgcaaaa cggagtaaga
    42781 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    42841 cgtcataaac gcgagttttt catgggtgat caggcgttct acgggggcgg aatcggcgaa
    42901 acctggaata atgtgttcca gactgtgggc atagagacgc ccgccagtaa cgttcttggc
    42961 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    43021 tgcggcaacc gaaccggcaa gccctgcacc gacgatgatg gcgtcaaaga tatcttcgga
    43081 cataagagct ccctgtcagc ggcagagccg taatgcagcc ctgccagagt ggatcaacgc
    43141 gctaaagctg cggtcagtgc cggaaggatc ttcacggcat cgccaacaat gccgtaatcc
    43201 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgacgcg
    43261 ttagcgccaa ccatgtgctg gatctacccg gagatcccca ccgccaggta cagttcaggt
    43321 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttct
    43381 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    43441 gcaatgttct ctttgctgcc aatgccgcga ccgacgctga ccaccagacg ggctttgtcg
    43501 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    43561 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    43621 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    43681 gccagaccgc cgtaaaccat gtgtttcact gttgctttac cgtcctgtac gctgacggtg
    43741 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    43801 ccgcgacggg tgtttggcaa cagcaccaga ccgtcagcgc cgtgctggcg aatagtgtca
    43861 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    43921 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggca
    43981 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agagggggta
    44041 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    44101 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    44161 gctgacgctc gcgctgtttc ggcgcggcaa cctgttgttc agaccaggcg tccacggcgt
    44221 taaaaccaat atccgccgcc gaccatacct ggactggctt tttcgccgcg ccgagaatgg
    44281 ctttcatcga aggaatttgt ggggagttga tatcagtgga aacagcaaca accgcaggca
    44341 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    44401 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    44461 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcagat
    44521 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    44581 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agcacatctt
    44641 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggctgtc acctgcgcct
    44701 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttgagatcg tattggctta
    44761 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    44821 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    44881 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    44941 taaaacttta atcaccaata ttgaaagtca gatgctattt ttttacccac aaaagaatat
    45001 aacaaactga ttaataacca aaattaacga aaaacgccct ctgttcactc gaaacaaaaa
    45061 tgtgatacca atcacagaat acagcttatt gaatacccat tatgagttag ccattaacgc
    45121 gtccatgatg ttaataataa ttatattaaa tgttaacaaa aataaaacaa acgggaacgc
    45181 aaaataaata ttcgttttca cagtggaatt aactcatgaa gaatgaaaag agaaaaacgg
    45241 gaatagaacc gaaggttttc tttccgccat taataatcgt cggcatactt tgttggctta
    45301 cagtcagaga tctggatgcg gcgaatgtcg ttattaatgc tgtattcagt tacgtcacca
    45361 atgtatgggg atgggcattt gaatggtata tggtggtgat gcttttcggt tggttctggc
    45421 tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga accgccagaa tttagcaccg
    45481 ccagttggat ctttatgatg ttcgcctcct gtacgtctgc tgccgtactg ttctggggat
    45541 cgattgagat ctactactac atctccaccc cgccgtttgg cttagaaccg aactcgacag
    45601 gagcgaaaga gttggggctg gcttacagct tgttccactg gggacctctg ccgtgggcca
    45661 cttacagctt cctttcagtc gccttcgctt acttcttctt tgtccgcaaa atggaagtga
    45721 ttcgccccag ctcgacactg gtgccgctgg caggtgaaaa acacgccaaa gggttgttcg
    45781 gtaccatcgt cgacaacttc tatctcgtcg ccttgatctt cgcgatgggt accagtctgg
    45841 gccttgccac gccgctggtg accgagtgta tgcaatggtt gtttggcatt ccgcataccc
    45901 tgcaactgga cgctatcatc attacctgct ggattatcct caacgccatt tgcgtcgctt
    45961 gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg tagttacctg agcttcctga
    46021 tgctgggttg ggtgttcatc gtcagcggtg ccagcttcat catgaactac ttcaccgatt
    46081 cggtggggat gttgctgatg tatctgccgc gcatgttgtt ctataccgat cccatcgcta
    46141 aaggcggctt cccgcagggc tggaccgtgt tctactgggc atggtgggtg atttacgcca
    46201 tccagatgag tatcttcctc gcccgcatct cccgtggtcg tactgtgcgt gaactgtgct
    46261 tcggcatggt gatggggctg acagcatcca cctggatcct gtggaccgtg ctcggtagta
    46321 acactctgct gttgatggat aaaaacatca tcaacattcc aaatctgatc gaacagtacg
    46381 gtgtggcgcg cgccatcatc gaaacctggg ccgctctgcc actcagcacc gccaccatgt
    46441 ggggcttctt catcctctgc tttattgcca ccgttacgct ggttaacgcc tgctcttata
    46501 ccctggcgat gtctacttgc cgcgaagtac gcgatggtga agaaccaccg ctgctggtgc
    46561 gtatcggttg gtcaattctg gttggcatta tcggtattgt tctgctggcg ctcggcggcc
    46621 tgaaaccgat tcaaaccgcc attatcgccg gaggatgccc gctgttcttc gtcaacatta
    46681 tggtgacgct ctcctttatt aaagacgcga aacagaactg gaaagattaa ttaaccccca
    46741 aaatatcaag aggttgaaag atggatttta atttaaatga tgagcaggaa ctgtttgtcg
    46801 ccggtatccg cgaactgatg gccagcgaaa actgggaggc ctattttgcc gagtgcgacc
    46861 gtgacagcgt ctacccggaa cgttttgtca aagcactggc ggatatgggt atcgacagtc
    46921 tgctgatccc tgaagagcac ggtggtctgg acgcggggtt tgttactctc gccgccgtgt
    46981 ggatggagct gggacgtctg ggggcaccaa cctatgtact gtaccagctg ccgggcgggt
    47041 tcaatacctt cctgcgcgaa ggcacacaag agcagatcga caagattatg gctttccgcg
    47101 gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc tccgacgtgg
    47161 gtagcctgaa aacgacttat acccgtagaa atggtaagat ttatcttaat ggtagtaagt
    47221 gttttattac cagtagcgcc tacaccccgt acatcgtggt gatggcgcgc gacggggctt
    47281 ctccggacaa acctgtctac accgaatggt ttgttgatat gagcaaaccg ggcatcaaag
    47341 tgaccaaact tgagaagctc ggtctgcgta tggatagctg ctgtgaaatc acctttgacg
    47401 atgtggaact ggacgagaaa gacatgttcg gtcgggaagg taacggcttt aaccgcgtca
    47461 aagaagagtt cgaccatgaa cgtttcctgg tagccctgac caactacggt acggcgatgt
    47521 gcgcctttga agatgctgcg cgctacgcca accagcgcgt gcagtttggc gaggctattg
    47581 gtcgtttcca gttgattcag gaaaaattcg cccacatggc gatcaaatta aactccatga
    47641 aaaacatgct gtatgaagca gcgtggaaag cagacaacgg caccatcacc tctggcgatg
    47701 cagcgatgtg caaatacttc tgcgccaatg cggcatttga agtggtggat agcgcaatgc
    47761 aggtgctggg cggtgtcggg attgcgggca accaccgcat cagccgcttc tggcgtgacc
    47821 tgcgtgtaga ccgcgtttcc ggaggatctg acgaaatgca gatcctgacg ctgggtcgtg
    47881 cggtgctgaa gcaataccgc taagtccgtg cgctgcctga tgcgacgctg acgcgtctta
    47941 tcaggcctac gggagcacga atgtaagccg gataaggcgt ttacgccgca tccggcagtc
    48001 atgcgctaca taaatttttc aggagctaat aatggatcat ctacccatgc cgaaattcgg
    48061 accgctggcc ggattgcgcg ttgtcttctc cggtatcgaa atcgccggac cgtttgccgg
    48121 acaaatgttc gcagaatggg gcgcggaagt tatttggatc gagaacgtcg cctgggccga
    48181 caccattcgc gttcaaccga actacccgca gctctcccgc cgcaatttgc acgcgctgtc
    48241 gttaaatatt ttcaaagatg aaggccgcga agcgtttctg aaattaatgg aaaccaccga
    48301 tatcttcatc gaagccagta aaggtccggc ctttgcccgt cgtggcatta ccgatgaagt
    48361 actgtggcag cacaacccga aactggttat cgctcacctg tccggttttg gtcagtacgg
    48421 caccgaggag tacaccaatc ttccggccta taacaccatc gcccaggcct tcagtggtta
    48481 cctgattcag aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata ccgccgatta
    48541 cttttctggc ctgaccgcca ccacggcggc gctggcagca ctgcataaag tgcgtgaaac
    48601 cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg atgctgcgta tgggccagta
    48661 cttcatgatg gattacttca acggcggcga aatgtgcccg cgcatgagca aaggtaaaga
    48721 tccctactac gccggttgcg gtctgtataa atgtgccgac ggctacatcg tgatggaact
    48781 ggtgggcatt acccaaattg aagagtgctt taaagatatt ggcctcgcac atctactcgg
    48841 tacgccggaa atcccggaag gcactcagct tatccaccgt atcgaatgcc cttacggccc
    48901 actggtagaa gaaaaactcg atgcctggct ggcggcacat accatcgcag aagttaaaga
    48961 acgctttgcc gaactgaata tcgcctgtgc caaagtactg accgtaccgg aactggaaag
    49021 caatccacag tatgttgccc gtgaatcaat cactcagtgg caaacgatgg atggtcgcac
    49081 ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac cccggacaaa tctggcgcgg
    49141 aatgccctca catggcatgg acacggctgc cattttgaaa aatatcggct acagcgaaaa
    49201 cgacattcag gagttggtca gcaaaggtct ggccaaagtt gaggactaaa cgcgtggcgt
    49261 cagtcggctt gcctgactag cgcccgccca ataacaaacg aacaaaaatg gatagaggtg
    49321 caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctg gcggacgttt
    49381 acggtcataa aacggcgctg atttgtgaat ccagcggcgg agtcgttaac cggtatagtt
    49441 atcttgagtt aaatcaggag attaaccgca cggcaaacct gttttatacg ctggggattc
    49501 gcaaaggcaa caaggttgca ctacatctcg acaactgccc ggaatttatc ttttgctggt
    49561 tcgggctggc aaaaattggc gcgattatgg tgccgattaa cgcccgcctg ttacgcgagg
    49621 aaagcgagtg gatcttgcaa aatagccagg cgtgcctgct ggtgaccagt gcgcaatttt
    49681 atcctatgta tcaacagatt cagcaggaag atgccactca attgcggcac atttgcctga
    49741 cagatgtggc acttcccgct gatgatggag tgagttcatt tactcaactg aaaaatcaac
    49801 aacctgccac cttgtgctat gcaccgccgc tatcgactga cgatacggcg gaaattctct
    49861 tcacctccgg caccacctcc cgaccgaaag gtgtggtgat tacccattac aacctgcgct
    49921 tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc tacatgacgg
    49981 taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg ttttctgccg
    50041 gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga caggcgcaga
    50101 agtaccgcgc caccattacc gaatgtattc cgatgatgat ccgtacgttg atggtgcagc
    50161 cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt gatgttttat ctcaacttgt
    50221 cggagcagga aaaagacgca ttttgtgaac gcttcggcgt tcgcttgctg acgtcttatg
    50281 ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tagcgataaa cgacgctggc
    50341 cgtcgattgg tcgggcgggg ttttgctacg aagcggagat ccgcgacgat cacaatcgcc
    50401 cgctcccggc aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct
    50461 tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc gacggctggc
    50521 tacataccgg cgataccgga taccgcgacg aagaaggctt tttttatttc gtcgatcgcc
    50581 gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg gaaaatatca
    50641 tcgccaccca tccgaaaatt caggacatcg tggttgtggg tattaaagat tcgattcgcg
    50701 atgaagccat caaagcattt gtggtgctga atgaaggtga aacattgagc gaagaggaat
    50761 tttttcgctt ctgcgaacaa aatatggcga aatttaaagt gccctcttat ctggagatca
    50821 gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg aaataaatga
    50881 aacgtcaggg aactaccctg ccagctaata atcacgccct aaaacaatat gcattttttg
    50941 cagggatgtt gtcgtcccta aaaaagcaaa aatggagaaa aggaatgagt gaatcattac
    51001 atctgacccg caatggatca attctggaaa ttacccttga tcgcccaaaa gcgaatgcta
    51061 ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt gacgatccgc
    51121 aattacgtgt cgccattatt accggtgccg gagagaaatt cttttccgcg ggctgggatt
    51181 taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccgggt ggttttgcgg
    51241 gattaaccga aattttcaat ctcgacaaac cagttatcgc agctgtgaac ggctacgcct
    51301 ttggcggcgg tttcgaactg gcgctggcgg cagattttat tgtttgtgcc gataacgcca
    51361 gcttcgccct gccggaagcc aaactgggca tcgttcctga cagcggcggt gtgctgcgtc
    51421 tgccgaagat cctgccgcct accatcgtca atgaaatggt gatgaccggc agacgaatgg
    51481 gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag gcggaactga
    51541 tggataacgc ccgcgaactg gctcagcagc tggttaacag cgccccgctg gcgattgcgg
    51601 cgctgaaaga gatctaccgc accaccagcg aaatgccggt agaagaagcg tatcgctata
    51661 ttcgcagcgg cgtgttgaaa cactatccat cggttctgca ttcggaagat gccattgaag
    51721 ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa caaccgtgag
    51781 ctattacgcc tttgaggggt taattccggt ggttcacccg acggcgtttg tccatcccag
    51841 tgccgtcttg attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc
    51901 actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg
    51961 cattatgcat ggctactgcg acactgacac catcgttggg gaaaacggcc atatcgggca
    52021 tggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt
    52081 aattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgcaatga gctttgtcaa
    52141 agcaggcttt agtggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtacg
    52201 cagtgttagt gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct
    52261 tgtcgggcgc tgtcatgcat cgttacatga aacgcagcca ctgaggcaaa tggaggaaaa
    52321 tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa cggtgatctt gccggatgcg
    52381 acctggttgc tggataagat gctacaacat cgtatctggc aagcaggacg cccggtatca
    52441 acgagataaa attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg
    52501 ttaagttccg tgagcggaac ggcaggaata cgcggtgtct ttcttacgcc gactaatttt
    52561 tcccgactgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cactttaacc
    52621 agtcgctgac actggcatcc ccgcccttcc agcttattag ggatcatctt aacttcgcag
    52681 cttatttctg tgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca
    52741 atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat
    52801 tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt
    52861 tcacaaatta aaagtctagc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa
    52921 attgtggata tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct
    52981 tatgtcatca aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaaacggtc
    53041 ctcatcagag aaccgtatta gttattcgat caaattagcg gatgaaaaat atccgccacg
    53101 acacgctatt atttgatctg tgcgtgcatt tcctgcaccg aaattacttt ttcagtcgca
    53161 tcggcattca gcgccatcgc ggtagcgaaa ccaccgttca gggtggtgtc ataatgcact
    53221 ttatattgca gcgcactgcg acggatcacg cgggagtctt caatcgcacg acggcctgag
    53281 gtggtgttga tgatgtaggt atattcgcca ttcttgatac ggtcctgaat gtgcggacgg
    53341 ccttcatgca ccttgtttac cagacgcgga ttgatacccg cttcgcccag cacaatcgcc
    53401 gtgccgtggg tcgcatccag ctcgaagccc tgtttcagca gttttgccgc caggtccacc
    53461 acgcgttctt tatcgccttc gcgcacggaa agcagcgcac gaccgtgttt cttcatggtg
    53521 gagttgctgc ccagctgcgc tttggcgaac gcttcagcga aggtgcggcc cacgcccatg
    53581 acttccccgg tagagcgcat ttctggccct aacagcgggt caacgcctgg gaatttattg
    53641 aacggcagca ccacttcttt caccgagtag tacggcggaa taacttcttt ggttacgccc
    53701 tgctcagcca gcgatttgcc agccatcaca cgcgccgcca cttttgccag cggtacgccg
    53761 gtggctttgg agacgaacgg aacagtacgc gccgcacgcg ggtttacttc aatcaggtaa
    53821 acttcgttgt ttttcaccgc aaactgcacg ttcatcagac cgcgcacctg caattcgaag
    53881 gccagtttct gcacctgctg gcgcatcaca tcctgaattt cctgacttaa ggtgtacgct
    53941 ggcagcgaac atgcggagtc accggagtgc acacccgcct gctcgatgtg ctccatgatg
    54001 ccgccaatca gcaccatttc gccgtcgcag atggcatcca cgtcaacttc tactgcgtca
    54061 tcaaggaaat gatccagcaa cactggcgca tcgttagaca cgctaaccgc cgtctggaag
    54121 tagcgacgca ggtcagcttc gtcatagacg atttccatcg cccgaccgcc gagaacgtaa
    54181 gacggacgca ccaccagcgg gtagccaatc tcttttgcct tctcaactgc catttcaata
    54241 gcagtaacgg tggcgttcgc cggttgtttc agtttcagac ggtcaaccgc atgctggaag
    54301 cgttcacggt cttccgcacg gtcgatagca tccgggctgg tgccgataac cggtacgcca
    54361 gcggcttcca gtgcacgcgc cagtttcagc ggggtctgac cgccgtactg aacgataacg
    54421 cctttcggct tctcaatacg cacgatttcc agcacatctt ccagagttac cggctcgaag
    54481 tagaggcgat cggaagtgtc gtagtcggta gagacggttt ccgggttaca gttaaccata
    54541 atggtttcgt aaccgtcttc acgcagcgcc agcgaggcgt gtacgcagca gtagtcgaat
    54601 tcgataccct gaccgatacg gtttggaccg ccgccaagca ccatgatttt ttcacggtcg
    54661 gtagacggat tcgcttcgca ctcttcttca taagtggagt acatgtaagc ggtgtcggtg
    54721 gcaaactctg ccgcacaggt atccacgcgc ttgtagaccg ggtgcaggtc atattggtca
    54781 cgcagcttac ggatttccgc ttcgcgtacg cccgccagtt ttgccaagcg cgcatcggca
    54841 aagcctttgc gtttcagctg gcgcaggaat tcagcgttca ggccagtgat gcccacttcc
    54901 gccactttct cttccagacg caccagttct tcaatctgta ccaggaacca gcggtcaatg
    54961 ttagtcaggt tgaagacgcc gtccacagac aggcccgcgc ggaaagcatc ggcgatgtac
    55021 cagatacgct ctgcgcctgc gtctttcagt tcgcgacgga ttttggttaa cgcttccggg
    55081 tcatccaggc tcactttcgg gtcgaagcca gttgcgccca cttccaggcc gcgcagcgct
    55141 ttttgcaggg attcctgctg cgtgcgacca atcgccatca cttcgccaac cgatttcatc
    55201 tgagtggtca gacggtcgtt agcaccggcg aatttttcga agttgaagcg aggaatttta
    55261 gtaaccacat agtcgatgga cggctcgaag gaggccggag tacgtccgcc agtgatgtcg
    55321 ttcatcagtt cgtctagggt gtaacccacc gccagtttcg ccgccacttt agcaatcggg
    55381 aaaccggtcg ctttcgacgc cagcgccgaa gaacgggaca cgcgtgggtt catttcgata
    55441 acaatcaggc gaccgttttt cgggttcacc gcaaactgga cattggaacc accggtttca
    55501 acgccgattt cacgcagcac cgccatcgag gcgttacgca tgatttgata ttctttgtcg
    55561 gtcagcgttt gggctggcgc gacagtgatg gagtcaccgg tgtggatgcc catcgcatca
    55621 aagttttcga tagagcagac gatgatgcag ttgtcgtttt tatcacgcac cacttccatc
    55681 tcgtactctt tccagccgat cagcgactca tcaatcagca actctttggt cggagagaga
    55741 tccagaccgc gggcgcaaat ttcttcaaac tcttcacggt tataagcgat accgccgccg
    55801 ctaccgccca tggtaaagga tgggcgaata atgcacggga agcccacgtc agcggcaaca
    55861 gccagcgctt cttccatcgt gtgtgcgata ccggaacgcg cggtttccag accaattttc
    55921 ttcatcgcta cgtcgaaacg acggcggtct tctgctttat caatcgcatc ggcagtggca
    55981 ccaatcatgg tgacaccgaa ctcttccaac acgccctgac gttccagctc cagcgcgcag
    56041 ttcagcgccg tctgaccgcc catggttggc agcaccgcgt ccgggcgctc tttttcaata
    56101 atcttgcgta ccacttccca gtgaatcggc tcgatgtagg tcgcatcggc catttccggg
    56161 tcggtcatga tggtcgccgg gttggagttc accagaatga cgcggtaacc ctcttcgcgc
    56221 agggctttac atgcttgcgc gccagagtag tcaaactcac acgcctgacc gataacaatc
    56281 gggcccgcac ccagaatcag gatacttttt atatctgtac gttttggcat ggctctttta
    56341 ctcctgatta cttagcggtt ttacggtact gctcaattaa cttgataaag tggtcgaaca
    56401 acggcgcggc gtcgtgtgga ccagggctgg cttcagggtg accctggaag ctgaacgccg
    56461 gcttatcgat gcgatgaatg ccctgtaacg taccgtcgaa cagggattta tgcgtgacac
    56521 gcaggtttgc aggtaatgtt gcttcatcca ccgcaaaacc gtggttctgg gcggtgatca
    56581 tcaccacgtt tttctccaca tctttaaccg gatggttgcc gccgtggtga ccaaatttca
    56641 ttttgacagt cttcgcaccg ctcgccagcg ccagcagctg atgaccgaga cagatgccga
    56701 ataccggaat atcggtttcg aggaacttct ggatggcggt aatggcgtaa tcgcacgggg
    56761 ccgggtcgcc aggaccgttg gagaggaaga tgccgtctgg attcattttc agcacatctt
    56821 ccgcagaagt ttgcgccgga acgatggtca ggcgacagcc tctatccacc agcatccgca
    56881 ggatgttgcg cttggcacca aaatcataag ccacgacgtg gaacggcagc tcgtcttctt
    56941 ttttcgcttc tggcaggccg ccggtcaacg tccagctccc ttgtgtccag ctataggctt
    57001 ctgcggtggt cacttctttt gccagatcca tgccgttcag acctgggaac gcgcgggctt
    57061 tttctaacgc cagcgccgca tccgggttat cgcctgcgat aatgcagcca ttctgtgcgc
    57121 ctttctcgcg cagtaaacgc gtcagcttac gggtatcgat atcggcaatc gccacgatgt
    57181 tatggcgctt caggtaagaa gagaggtctt cggtattacg gaagttgctg gcaatcagcg
    57241 gcaggtcgcg aatcaccaga ccttgtgcat gtacctgaga agattcttca tcggcgtcat
    57301 tagtgccgac attgccaata tggggataag taagagtaac gatttgacga gaataggaag
    57361 gatcagtgag gatttcttga taaccggtca ttgaagtatt gaaaacgact tccccaaccg
    57421 ccgaacctgt tgcccctatg gcccgaccgt gaaactgggt tccgtcttcc agaaccaata
    57481 gcgctgactt aatcaaaaca ccctccagag aatactcact cactttattt gcatattaat
    57541 tcacagtgat gacgtaaatc aatgcaaatc tgcttaccga cgaatttctg gcaaacggcg
    57601 gcattctgga gatatgggcg ctaaaagtca acttaatggc aggatttttt atattatttt
    57661 gtggctcatg catgttttac aggcattaag cgacaaaaac atcatctaac ctgttctgga
    57721 aaacgcttgc gcagcaagaa gattgcatga aataaaaacg agcaggaaaa agtggaccaa
    57781 atggtcaaaa tttacattaa aacaacaaaa atagaagacg aaacatgtaa tttttggcag
    57841 ttatagaaaa tttaaaagca taacaccaaa aataaaaggg ctaaaaatta aagcccttta
    57901 taattaataa attaaatgtg ttacttttgc atcataataa acattttgtg gttacaaatt
    57961 attgagatca agcacatctc gcatatcaaa aagaccactt tccttaccac tcaaccacaa
    58021 agccgatctt accgcaccgt tagcaaatgt catgcggctg gacgccttat gggtgatctc
    58081 cagacgctcg ccaatatcgg caaacatcgc ggtatgttcg ccaacgatgt cacctgcacg
    58141 cacggtagca aaaccaatgg tacctggcac acgttcaccg gtatggcctt cacgactgta
    58201 gaccgcgcaa tctttcagat ctttatcaag ggcgtgggcg atcgcctctc ccattgccag
    58261 tgcggtgcct gacggcgcat caactttatg tctatgatgt gcttcaataa tttcgatatc
    58321 ggtgtagtca cccatcactt tggctgcttt ctccagcagc ttaagcatga cgttaacgcc
    58381 aacgctaaaa ttggcggcaa agacaatcgc aatatcggcg gcggcgtcac gaattgcctg
    58441 tttaccggct tcgtcaaacc ccgtagtgcc gatcaccatc cctttgccat gctgccgaca
    58501 aaaagcgaga tggttcagcg taccttccgg acgggtaaaa tcgataaaca catcaaaatc
    58561 atcttttatc gcatcaagac tgctttgcac ggtaacgcct gttttccctg caccagccag
    58621 ctcaccggcg tcgctgccca gtaaagaaga tccttcgcgc tccagcgccg cgcccagctg
    58681 cacgccttct aatgccagtg ccgcctgaat caactggcgg cccatacgcc ccccggctcc
    58741 cgcgatggca acgcggatgt ttgcatcatg catagctatt ctcttttgtt aatttgcata
    58801 gaccgttttc agagtaacca gcgcatcgct acgccgccag ccaaaaacgc tgataattag
    58861 aaattaatga taaacaggga gggatatcag tgaaaggcat gagtcgatga atgactgcat
    58921 gccgataaca tgtgacaggt tacggcacca gagccagcac ctcagccacc cactgttgga
    58981 agccttttac attcagatcc aatgccacct gtacattggc tggtttgccc aggcaaccgt
    59041 cgatatcaac caccgtcgtg cctgaggtaa attcgccttg agtttctacc gcgacaaaac
    59101 acggcttgag ggtgaataag tccgggcgca ccagccaggc gatggcgcag agatcgtgca
    59161 ttcgcagacc gctttgcata ctgccgctac ggtagtggct aaacagggcg tggagcattt
    59221 ttccggtgcg attcaactcc ggtaaagtag cgagatagtc aggggttaat attgcctgat
    59281 tggtgacatc cagaccgcac atgacaattt cgatgccgct acggaagaca caggcagccg
    59341 cttctggatc ggcagcgata ttaaactcgg catttggtgt acagttgccg cgaccagcag
    59401 aaccgcccat gatcaccaga cgacgaatat acggtttaca ttccgggcat tgtaaaagta
    59461 acagcgcaat attggttaac gggccgatgg ccaccagggt aacgggctct ggtgcacgca
    59521 tcagggcatc ccgaatcgcc agaaacgccg gtatcccgag tggctttcgg ttgtgctcaa
    59581 caaagtcgta gccagccatt cccgattcgc cgtgcacaga tgctgcatca cgcggtgcgc
    59641 gtaccagcgg cacagcggct ccctgggcga gcggaatctc cgcattccag aaatgcagca
    59701 gttgcagggc attgcgggta gttttctcaa ccgagacatt acccgcgacg gtggtcatca
    59761 gttgcaggtc gagttcgggg gcaaaaatcg cggcggcaat ggcgacggca tcgtcaatgc
    59821 cggggtcagt atcgaggaag ataggtaaac gcatgttttc ttcataaaaa atgccggtaa
    59881 caagaccggc attttcgcat aacttaggct gctaatgact taatcgactt cacgaatatc
    59941 gacacgcagc tctttcggca cttcgaaaac aatattttct tcacggcctt ccagcggaat
    60001 ggcttcacca ccacccaact gctgcaaacg tgccaccaca ttctgcacca gaatatccgg
    60061 agccgatgcg cccgcagtca cgccgacgca tttaacctct ttcacccact cttcctggat
    60121 atctttcgca tcgtcaatca aaaacgcgtg tttgcccata cgctgggcca gctccgccag
    60181 acggttggag ttggaggagt ttttcgaacc gaccaccaac acaacttccg cctgttctgc
    60241 cagggcgcgt accgcttcct gacggttagt cgtggcgtag cagatgtcat ctttgcgcgg
    60301 accgacaatt ttcgggaagc gtttacgcag cgcgtcgatc acatcagacg tgtcatccac
    60361 cgacagcgtg gtttgggtca taaaggagag cttctcttcg tttttgaccg tcagtttcca
    60421 cacatcgtct ggcgattcga ctagatacat tcccccttcg gggttgctgt actgccccat
    60481 cgtcccttcc acttccgggt gcccggcgtg gccgatgaga atagattctt cgccacggcg
    60541 actggcgcgg gcaacttcca tatgcacttt ggtcaccagc ggacaggtgg catcaaacac
    60601 cgtcaaatcg cggcttttcg cttcgttacg aaccgcctga gaaacaccgt gtgcggagaa
    60661 aatcaggatc gcgccgtccg gcacttcgct aatctgctca ataaagatag ccccacgctc
    60721 gcgcaagcta tcgaccacgt agcggttatg caccacttcg tgacggacat atatcggtgc
    60781 gccgtaaatg gccagcgcgt tttcaacaat gctgatagcg cggtctaccc cggcacaaaa
    60841 accacgcggg ttggccaaca ggatctgcat gttacgcctc cagtgccgga tcgatttcca
    60901 gcacttcaat atcaaaatga acggtctgcc cggccagcgg atggttgaaa tcaacggtaa
    60961 tggagtcgcc gttaatttcg cggatcacac caggcatctc actgccatcc attgcggtaa
    61021 aaagcatgat tgcgccaatt tctggctcgc ctgcatccat aaattcacgg cgggagaagt
    61081 actgaatcag gtccggtgac ggcacgccaa acgccgcatc tggctccagc gagaaggtgg
    61141 ttttgtcgcc cactttcagc cccagcaggt gttgctccag cccttcagaa agagaagcat
    61201 cgcccaggcg gaacagcgcc ggtttaccgt tgttgcgagt agactcggcg gtggtgccat
    61261 cgtcgagttt tagcgtgaag tgcaccagga cggcgctatt gctctgtaca gattcagaca
    61321 tgcaggtttg ctcttatttt tgaggatttt tgccggatgc ggcctacgat ttcgtagcaa
    61381 tctgtaggcc gcatccggca ggatttatta ttgttttttc gctttggaag gtaaaaaccc
    61441 ttccagcacg atcagtgccg caccgacaca aatggcagta tcggcaaggt tgaaggtggc
    61501 gaagtgccag tcgccgacgt agaagtcgat catatcgaca acgaagccgt gccacaggcg
    61561 gtcgaacagg ttgcccagcg cgccgccaat aatcagcgcg taagcgatat tgtttagctt
    61621 ctgcgtggcc ttcgagcgat acatcatcac tgccaggagc acgctaatac caatcgcaat
    61681 accggcaaag aaccaacgct gccagccgcc gctatcggca aggaaactaa acgccgcgcc
    61741 atagttacgc gcataatgca gattaagcga cgggaacagc gggaccgtat cccccagagc
    61801 aaagttctgg aggatcaggt atttgctgcc cagatcgata atcagcacga ctaccaccag
    61861 ccacagccag cgtagccctg ttgaacagat cgattgactc atcaggcaaa cttacgtttt
    61921 tcaccgtcac cggcgacgtt gctgacacag cggccgcaga tttctgcgtg ttccgccacc
    61981 ttgccgacat cctgggtgta gtgccagcag cgtgggcact tctcaccttc ggctttactc
    62041 aacgcgactt tcagcccttt gagtacttcg ctctgctgag catcagcagg tgcgtcgtta
    62101 tagtctgcaa cggtagcgcc ggaggtcaac aggacaaatc gtaattcatc gcccagcgcg
    62161 gtcagtttcg ccgccagttc cggttctgca tacaaggtta ctgccgcttc cagcgagcca
    62221 cccactttct tgtcggcacg cgcttgctca atgactttgt tcacttcgcc acgcactttc
    62281 aacagctcgt cccagaacgc atcgttcatt gcttcactgt ctgccagacc aaacaggcct
    62341 tcgtaccact caccggtgaa gacgtatttt tcacgttcgc ccggcagata gccccacact
    62401 tcatcagcgg tgaaggagag gattggtgcc atccagcgca ccagcgcttc agcgatgtga
    62461 tacagcgcag tctggcagct acgacgcgcc acgctatccg ctttggcggt gtactgacgg
    62521 tctttgatga tgtcgaggta gaaggaaccc atctcaacgg agcagaagcg catcagacgc
    62581 tgtaccactt cgtggaaatc gtatgcttcg tacgccttga ggatgtcttc ctgtgccgct
    62641 ttcgcacaac ctacggccca gcgatccagt accaccatct cttccggttt caccatatct
    62701 tttgctggat caaaaccatt caggtttgcc agcaggaagc gcgcggtgtt acggatacga
    62761 cgatagctat cggcagcacg tttcaggatc tcgtcagaaa cggccatttc accggtgtag
    62821 tcggttgatg ccacccacag acgcagaata tccgcgccca gtttgttcat cacatcctgc
    62881 ggcgaaacgg tgttgccgat ggatttagac atcttgcgac cctgaccatc cacggtaaag
    62941 ccgtgggtca gtacctgacg atacggcgct ttgcctttca tcgcggtgga gatcattagg
    63001 gaagacatga accaaccacg gtgctggtca gaaccttcca gatacatgtc cgctgcgtga
    63061 ccggcaaatt ccggacgcac gtcaacaaca gaagagtggg tagatccgga gtcaaaccat
    63121 acgtcgagag tatccggcac tttcacatac tggtcagctt cgtcgccgag gatctctttc
    63181 gcatcgagat cccaccacgc ctggatgccg tcaacttcaa cgcgtttagc cacttcttcc
    63241 atcagttcga gggtacgcgg atgcagctct tccgtgtctt tgtgcacgaa cagtgacatc
    63301 ggtacgcccc aggtgcgctg acgggagata caccagtcag gacggttagc aaccatcgac
    63361 tcgatacgcg cctggcccca gtccgggagc cactgcacgc ctttgatctc tttcagtgac
    63421 tgcgcacgca gacctttctg atccatgctg acgaaccact gcggtgtcgc gcggaagatg
    63481 atcggcgttt tgtgacgcca gcagcacgga tagctgtgct gcattttctc aacgtgcagc
    63541 agcgcgcctt tttcctgcag cagcgcaacg acgatgtcgt tcgctttgaa gacgttcacg
    63601 ccatccagcg tcggataagt gcccggcaga taagtgccgt ccgggccaac cgggttagcg
    63661 gtttccaggc cgtatttctg accgatcaca tagtcgtccg ggccgtggcc aggcgcggtg
    63721 tgaacggcac cggtaccggc atccagggta acgtgatcgc cgaggattgc cggaacgtcg
    63781 aagcccataa acggatgggt aaagcgcagc agctcaagct ctgcaccttt taccgtgccg
    63841 agaatggtgt aatcggtcac gccgatacgc tgcattacgc tttcaaccag atctttcgcc
    63901 agaatcacgg cctgaccgtc gatctgcacc agcgcatagt cgaaatcagg tgcaatagag
    63961 attgcgcggt tcgcaggcag agtccacggc gtagtggtcc agatcaccag cgagattggg
    64021 ccgttaacgt tgcttacgcc aaatttcgct ttcagcgcat cctgatcgac cgcctggaaa
    64081 gcgacgtcaa tggacggaga agttttgtcg taatactcaa cttccgcttc tgccagtgca
    64141 gaacggcagt ctacgcacca gtgcaccggc ttcgcgcctt tgtgcaggtg accattgccg
    64201 atgattttgc ccagcgcgcg gatgatgttg gcttcagttt tgaagtccat agtcagatac
    64261 gggtgcgacc agtcgcccag cacgcccaga cggataaagt ctttgcgttg accgtcaacc
    64321 tgggtcgccg cgtattcgcg gcacttggcg cggaactcgg cggcggtgaa tttctcaccc
    64381 ggcttaccgt attcttgctc tactttcagc tcgatcggca gaccgtggca gtcccagcca
    64441 ggcacatacg gcgagtcata accggaaagc cctttggact tcacgataat gtctttcaga
    64501 atcttgttaa ccgagtgacc aatatgaatg ctgccattcg cataaggagg gccatcatgc
    64561 agaatgaagg tttttttgcc ttttttagcc gcacggatga tgccgtacag atcatcatca
    64621 gtccaacgcg ccagcattcc cggttcgcgc ttggcgagat cgccacgcat cgggaaccct
    64681 gtttccggca aattcagggt tgatttatag tcactcatca gattctcggt tccgtgtttc
    64741 ggtttgatta cataacaggc ttaagccggt tttgttagcc caaaaaattc gcgggcggtt
    64801 aattcatcac gcgcaatctg cgctttcagt tcgtccagcg acgcaaatcg ctgctcattg
    64861 cgtatttttt tacgcagcac tacttgtata tggcgaccgt aaaggtccat tgcaacatct
    64921 aacaaatgca cttccagctg ctggcgaata ccggcaaccg ttgggcgtgt tccgatgttt
    64981 gccacgccgg gtaacggctt ttcaccgagg cccagcactt ctaccgcata aacccctttc
    65041 accggggaaa cctgacggcg tagcggtaca ttcgccgtcg ggaaacctat agtgcgccct
    65101 aattcatcac cgtggactac acgcccggag atggcaaacg ggtgccccag taaactttct
    65161 gccagagcca gattg
//
LOCUS       DAJTYM010000008        64144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTYM010000008 DAJTYM010000000
VERSION     DAJTYM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..2402)
                     /gene="glgP"
                     /locus_tag="OO575_000525"
     CDS             complement(<1..2402)
                     /gene="glgP"
                     /locus_tag="OO575_000525"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6663615.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TI"
     gene            complement(2421..3854)
                     /gene="glgA"
                     /locus_tag="OO575_000526"
     CDS             complement(2421..3854)
                     /gene="glgA"
                     /locus_tag="OO575_000526"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6663616.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3854..5149)
                     /gene="glgC"
                     /locus_tag="OO575_000527"
     CDS             complement(3854..5149)
                     /gene="glgC"
                     /locus_tag="OO575_000527"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6663617.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5167..7140)
                     /gene="glgX"
                     /locus_tag="OO575_000528"
     CDS             complement(5167..7140)
                     /gene="glgX"
                     /locus_tag="OO575_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6663618.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7137..9323)
                     /gene="glgB"
                     /locus_tag="OO575_000529"
     CDS             complement(7137..9323)
                     /gene="glgB"
                     /locus_tag="OO575_000529"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6663619.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9596..10699)
                     /gene="asd"
                     /locus_tag="OO575_000530"
     CDS             complement(9596..10699)
                     /gene="asd"
                     /locus_tag="OO575_000530"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6663620.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10891..11484
                     /gene="yhgN"
                     /locus_tag="OO575_000531"
     CDS             10891..11484
                     /gene="yhgN"
                     /locus_tag="OO575_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6663621.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11541..12881)
                     /gene="gntU"
                     /locus_tag="OO575_000532"
     CDS             complement(11541..12881)
                     /gene="gntU"
                     /locus_tag="OO575_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6663622.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12885..13412)
                     /gene="gntK"
                     /locus_tag="OO575_000533"
     CDS             complement(12885..13412)
                     /gene="gntK"
                     /locus_tag="OO575_000533"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6663623.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13551..14546)
                     /gene="gntR"
                     /locus_tag="OO575_000534"
     CDS             complement(13551..14546)
                     /gene="gntR"
                     /locus_tag="OO575_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6663624.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14770..15465)
                     /gene="yhhW"
                     /locus_tag="OO575_000535"
     CDS             complement(14770..15465)
                     /gene="yhhW"
                     /locus_tag="OO575_000535"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6663625.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15588..16625)
                     /locus_tag="OO575_000536"
     CDS             complement(15588..16625)
                     /locus_tag="OO575_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6663626.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            16958..17446
                     /gene="yhhY"
                     /locus_tag="OO575_000537"
     CDS             16958..17446
                     /gene="yhhY"
                     /locus_tag="OO575_000537"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6663627.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17505..18887)
                     /gene="ggt"
                     /locus_tag="OO575_000538"
                     /pseudo
     CDS             complement(17505..18887)
                     /gene="ggt"
                     /locus_tag="OO575_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(18885..19238)
                     /gene="ugpC"
                     /locus_tag="OO575_000539"
                     /pseudo
     CDS             complement(18885..19238)
                     /gene="ugpC"
                     /locus_tag="OO575_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
     gene            complement(19240..20085)
                     /gene="ugpE"
                     /locus_tag="OO575_000540"
     CDS             complement(19240..20085)
                     /gene="ugpE"
                     /locus_tag="OO575_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6663628.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(20082..20969)
                     /gene="ugpA"
                     /locus_tag="OO575_000541"
     CDS             complement(20082..20969)
                     /gene="ugpA"
                     /locus_tag="OO575_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6663629.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(21067..22383)
                     /gene="ugpB"
                     /locus_tag="OO575_000542"
     CDS             complement(21067..22383)
                     /gene="ugpB"
                     /locus_tag="OO575_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6663630.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(22780..23493)
                     /gene="livF"
                     /locus_tag="OO575_000543"
     CDS             complement(22780..23493)
                     /gene="livF"
                     /locus_tag="OO575_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6663631.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(23495..24262)
                     /gene="livG"
                     /locus_tag="OO575_000544"
     CDS             complement(23495..24262)
                     /gene="livG"
                     /locus_tag="OO575_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6663632.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(24259..25536)
                     /gene="livM"
                     /locus_tag="OO575_000545"
     CDS             complement(24259..25536)
                     /gene="livM"
                     /locus_tag="OO575_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6663633.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(25533..26459)
                     /gene="livH"
                     /locus_tag="OO575_000546"
     CDS             complement(25533..26459)
                     /gene="livH"
                     /locus_tag="OO575_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6663634.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(26507..27616)
                     /gene="livK"
                     /locus_tag="OO575_000547"
     CDS             complement(26507..27616)
                     /gene="livK"
                     /locus_tag="OO575_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6663635.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            28040..28423
                     /gene="panM"
                     /locus_tag="OO575_000548"
     CDS             28040..28423
                     /gene="panM"
                     /locus_tag="OO575_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6663636.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(28622..29725)
                     /gene="livJ"
                     /locus_tag="OO575_000549"
     CDS             complement(28622..29725)
                     /gene="livJ"
                     /locus_tag="OO575_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6663637.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(29996..30850)
                     /gene="rpoH"
                     /locus_tag="OO575_000550"
     CDS             complement(29996..30850)
                     /gene="rpoH"
                     /locus_tag="OO575_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6663638.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(31095..32153)
                     /gene="ftsX"
                     /locus_tag="OO575_000551"
     CDS             complement(31095..32153)
                     /gene="ftsX"
                     /locus_tag="OO575_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6663639.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(32146..32814)
                     /gene="ftsE"
                     /locus_tag="OO575_000552"
     CDS             complement(32146..32814)
                     /gene="ftsE"
                     /locus_tag="OO575_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6663640.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(32817..34313)
                     /gene="ftsY"
                     /locus_tag="OO575_000553"
     CDS             complement(32817..34313)
                     /gene="ftsY"
                     /locus_tag="OO575_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6663641.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            34463..35059
                     /gene="rsmD"
                     /locus_tag="OO575_000554"
     CDS             34463..35059
                     /gene="rsmD"
                     /locus_tag="OO575_000554"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6663642.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            35049..35318
                     /locus_tag="OO575_000555"
     CDS             35049..35318
                     /locus_tag="OO575_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6663643.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(35321..35680)
                     /locus_tag="OO575_000556"
     CDS             complement(35321..35680)
                     /locus_tag="OO575_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6663644.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            35821..36447
                     /locus_tag="OO575_000557"
     CDS             35821..36447
                     /locus_tag="OO575_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6663645.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            36521..38719
                     /gene="zntA"
                     /locus_tag="OO575_000558"
     CDS             36521..38719
                     /gene="zntA"
                     /locus_tag="OO575_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6663646.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(38821..39066)
                     /gene="tusA"
                     /locus_tag="OO575_000559"
     CDS             complement(38821..39066)
                     /gene="tusA"
                     /locus_tag="OO575_000559"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6663647.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            39287..39952
                     /locus_tag="OO575_000560"
     CDS             39287..39952
                     /locus_tag="OO575_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6663648.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            40025..40582
                     /gene="dcrB"
                     /locus_tag="OO575_000561"
     CDS             40025..40582
                     /gene="dcrB"
                     /locus_tag="OO575_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6663649.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(40586..41803)
                     /locus_tag="OO575_000562"
     CDS             complement(40586..41803)
                     /locus_tag="OO575_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6663650.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            41937..42986
                     /locus_tag="OO575_000563"
     CDS             41937..42986
                     /locus_tag="OO575_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6663651.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            43041..43628
                     /gene="acpT"
                     /locus_tag="OO575_000564"
     CDS             43041..43628
                     /gene="acpT"
                     /locus_tag="OO575_000564"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6663652.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            43739..45313
                     /gene="nikA"
                     /locus_tag="OO575_000565"
     CDS             43739..45313
                     /gene="nikA"
                     /locus_tag="OO575_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6663653.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            45313..46257
                     /gene="nikB"
                     /locus_tag="OO575_000566"
     CDS             45313..46257
                     /gene="nikB"
                     /locus_tag="OO575_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6663654.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            46254..47087
                     /gene="nikC"
                     /locus_tag="OO575_000567"
     CDS             46254..47087
                     /gene="nikC"
                     /locus_tag="OO575_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6663655.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            47087..47851
                     /gene="nikD"
                     /locus_tag="OO575_000568"
     CDS             47087..47851
                     /gene="nikD"
                     /locus_tag="OO575_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6663656.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            47848..48654
                     /gene="nikE"
                     /locus_tag="OO575_000569"
     CDS             47848..48654
                     /gene="nikE"
                     /locus_tag="OO575_000569"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6663657.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            48660..49061
                     /gene="nikR"
                     /locus_tag="OO575_000570"
     CDS             48660..49061
                     /gene="nikR"
                     /locus_tag="OO575_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6663658.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(49181..49540)
                     /locus_tag="OO575_000571"
     CDS             complement(49181..49540)
                     /locus_tag="OO575_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6663659.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(49537..49812)
                     /locus_tag="OO575_000572"
     CDS             complement(49537..49812)
                     /locus_tag="OO575_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6663660.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(49871..50995)
                     /locus_tag="OO575_000573"
     CDS             complement(49871..50995)
                     /locus_tag="OO575_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6663661.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(50995..53730)
                     /gene="rbbA"
                     /locus_tag="OO575_000574"
     CDS             complement(50995..53730)
                     /gene="rbbA"
                     /locus_tag="OO575_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6663662.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(53727..54794)
                     /locus_tag="OO575_000575"
     CDS             complement(53727..54794)
                     /locus_tag="OO575_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6663663.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(55160..56782)
                     /locus_tag="OO575_000576"
     CDS             complement(55160..56782)
                     /locus_tag="OO575_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6663664.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(57044..58633)
                     /locus_tag="OO575_000577"
     CDS             complement(57044..58633)
                     /locus_tag="OO575_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6663665.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            59018..60049
                     /locus_tag="OO575_000578"
                     /pseudo
     CDS             59018..60049
                     /locus_tag="OO575_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(60136..60612)
                     /locus_tag="OO575_000579"
     CDS             complement(60136..60612)
                     /locus_tag="OO575_000579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663666.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(60605..61369)
                     /locus_tag="OO575_000580"
     CDS             complement(60605..61369)
                     /locus_tag="OO575_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6663667.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            61615..62376
                     /locus_tag="OO575_000581"
                     /pseudo
     CDS             61615..62376
                     /locus_tag="OO575_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(62483..62983)
                     /locus_tag="OO575_000582"
     CDS             complement(62483..62983)
                     /locus_tag="OO575_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663668.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(63134..>64144)
                     /locus_tag="OO575_000583"
     CDS             complement(63134..>64144)
                     /locus_tag="OO575_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6663669.1"
                     /translation="NIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKN
                     DLLTSHKWKVDRKLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGV
                     WWDVATNITNAHQSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILIT
                     RDGLTFGQNVDGTLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEK
                     TIGKNYDLPQTSQLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKD
                     TENMAGIVGEDGIAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAAT
                     CL"
BASE COUNT        15612 a        17151 c        15709 g        15672 t
ORIGIN      
        1 ttgatagtac ggtcagaaga gaagtagccc atattggcaa tgttcagcat cgcttttgcg
       61 gtccactctt cctgaagctc gtagagttcg tcgactttat cctgacaatc gacatagctg
      121 cgataatccg ccagtacctg gtagtgatcg ccgaagttga tcagcgaatc aaccagatcg
      181 cgatagcgac ccggatcttc cggactgaat acaccgctgc cgatttgcgt cagcacctga
      241 tgcagctcct catctttctc gtagtattca cgcggtttgt agccctgacg acgcagttct
      301 tccacttctt ccgctgtgtt accaaaaata aagatattgt cagcaccgac atgatccagc
      361 atctcgacat tcgcaccgtc caacgtaccg atagtcagcg caccgttaag cgcaaacttc
      421 atgttactgg tgccggaagc ttccgtccct gccagcgaaa tctgttcaga cagatctgcc
      481 gccggaatga tcaactgcgc caggctaacg ctgtagttcg ggatgaacac gactttcagc
      541 ttatcgccaa tctgcggatc gttgttgatc actttcgcta cgtcattgat caaatgaata
      601 atgtgcttcg ccatgtaata ggccgaagcc gccttaccgc caaaaatatt cacgcgcggt
      661 acccacttcg catccgggtc ggccttgatg cggttatagc gggtaatcac atgcaacaca
      721 ttcatcaatt gacgtttgta ttcgtgaata cgtttgattt gtacatcgaa caacgccttt
      781 ggattcacca ccacattcag ctgctgggcg atatactctg ccagacgctt tttgttctcc
      841 agcttcgcct gatgcacagc gtgattaacc attgggaaat cacagtgttg ttgcagctca
      901 ttaagcaggc taaggtcggt gcgccagtta cggcccaggt gttcgtccag cacggctgaa
      961 agcgatgggt tcgctaccgc cagccagcga cgcggcgtca caccgttggt gacgttggtg
     1021 aaacgacccg ggaagatttt cgcaaagtcg gcaaacaacg attgcaccat cagattagag
     1081 tgcagttccg atacaccgtt aactttgtgg ctcacaacaa ccgccagcca ggccatacgc
     1141 acacgacgac cgttggattc atcaatgatc gacgcccgtc ccagcagatc ggtatcgttc
     1201 ggatactgtt cctgcaaggt tttcaggaaa tagtcgttga tttcaaagat gatctgcagg
     1261 tgacgcggca gaattttacc cagcatatca accggccagg tttccagcgc ctcgctcatc
     1321 agcgtgtggt tagtgtagga gaagacctga caacacacct caaacgcgtc gtcccagcta
     1381 aattggtgct catcgatcag cagacgcatc atctcaggaa tcgacagtac cggatgggta
     1441 tcattgagat gaatcgcgat tttatccgcc aggttatcgt aggttttatg caactgataa
     1501 tgacggctta aaatgtcctg aatggtcgag gaaaccaaga agtattcctg acgcaggcgc
     1561 agctcacgcc cggagtaggt ggagtcatcc ggatacagta cgcgagatac gttctcggag
     1621 tggtttttat cttccactgc cgcgaagtag tcaccctggt tgaatttacc gaggttaatt
     1681 tcgctactgg cttgcgcact ccacaaacgc agcgtgttgg tcgcgtcggt gtcgtaacca
     1741 gggattatct gatcgtaagc gactcccaga atctcttcgg tttcaatcca gcgcgttttt
     1801 ttaccttcct gctgaatgcg accaccaaaa cggactttat agcgcgtgtt gtggcgtttg
     1861 aattcccacg ggttaccgta ttccagccag tagtctggcg actctttctg gctaccgtta
     1921 acgatgttct gcttgaacat accgtagtca tagcggatgc cgtaaccgcg ccccggcaac
     1981 cctaacgtcg ccagagaatc aaggaagcaa gccgccagac gtcccaggcc accgttaccg
     2041 aggcctgggt cattttcttc atcaatcagc tcttcgagat ttaaccccat cgcttccagt
     2101 gcgccctgta catcttcgta aattcctagc gacaacatgg cgttggagag cgtacggcca
     2161 atcaaaaact ccatcgacag gtagtaaacc tgacgagttt cttgcgacaa ctgggcacgg
     2221 tttgaacgta accagcgctc cacgagacga tcgcgcacag caaataacgt tgcgttcagc
     2281 cattcatgtt tattggcgac gaccgggtcc tttccaatcg taaacatcag cttgtaagcg
     2341 atagagtgct taagagcttc tacgctaagc gtgggcgatg aatatgtaaa cggagcattc
     2401 atataggcgt ttcctgaaaa ctatttcaag cgatagtaaa gctcacggta cgacttcgcc
     2461 gcgacctgcc agctaaaatc cattgccata gcctgacgtt gcacaaaccg ccacagtgaa
     2521 ggacgggacc acagtacaaa agcacgccga atagcccgta acagcgacca ggcattacta
     2581 tcttcaaaga caaacccact ggcgacgcca tctgcaaggt tctcaagaga acagtcagaa
     2641 accgtatcag caagcccacc ggtgcgccgc actaacggca gcgtaccgta cttcaatcca
     2701 taaagttgcg ttaagccgca cggttcaaaa cggctgggca ccagaatgac gtccgcgccg
     2761 cccataatgc gatgcgaaaa tgcttcgtga tagccaatct gaacgcccac ctgaccgggg
     2821 tattccgctg ccgccgcaag gaaaccttcc tgcagcaccg gatcgcccgc gccgagtagc
     2881 gccagctgcc cgccctgctc cagaagcccc ggtaaggctt ccagcaccag gtcgagacct
     2941 ttctggctgg tcagacggct caccactgca aaaagcggca ctttatcgtc aaccttaagc
     3001 cccattgcga tttgtaactg gctcttattt tccgctttat cttccaacgt atcgcgggtg
     3061 taacgcgagg ccaacagtaa gtccgtctct ggactccaga ttttctcgtc cacgccgttc
     3121 agtacgcctg aaagacgccc ttcacggtga cgctgttgca acagaccttc cataccgtag
     3181 gcaaactgcg gttcggtgat ctcgcgagcg taggttggac tgaccgccgt aatgtgatcg
     3241 gcatagtaca gaccggcctt caggaaagag atttgtccgt tgaattccag cccatgaata
     3301 ttaaagaatg accatggcaa ttggatgtca ttcatgtgat gtgcataaaa catgccttga
     3361 taggccaggt tgtgcacagt aaacaccgac ttcgcaggac gcccgcgcgc cgccagatac
     3421 gcaggcgcaa gacctgcatg ccagtcgtgc acatgcacca catcaggacg ccagaatggg
     3481 tcaagcccgc tggccatttc tgccccaacc caccccagca gcgcaaaacg caatacgttg
     3541 tcggtatagg caaataagtt ggtatcgtga tacgggcttc ccggacgatc atagagatgc
     3601 ggcgcgtcaa tcaggtatat gccaaccccg ttgtaatgac cgaacaacag cgtgatatgc
     3661 ccggcgaaag tatcacgacg ggatactacc tgcgcatcgg tcacgccacg gcgaatatcg
     3721 ggaaatgcag gcaacagtac gcgagcgtca acgccgtctg cgatttgtgc tgcgggtaat
     3781 gccccaataa catcagccag accgccggtt ttaagcagcg ggaacatctc tgaacataca
     3841 tgtaaaacct gcattatcgc tcctgtttat gccctaactt ccgtagcatt tcgcgcgtta
     3901 ccagcacgat gccttcttct gaacgataga aacgacgtgc atcttcctct gcgttttcac
     3961 caatcaccat gccttccggg ataacacaag cacgatcgat gacgcagcgg cgcagacggc
     4021 acgagcgacc tacccatact tccggtaaca atacggcgga atcaatgttg cagaatgaat
     4081 tcacgcgaac gcgcgagaac agaacggact gcaccaccac cgaaccggag atcacacaac
     4141 cgccggatac cagtgagtta agggtcatcc cgtggctacc ggagcgatcc tgcacgaatt
     4201 tcgctggcgg taatgattca ttgtaggtgc gaattggcca attgcgatcg tacatatcca
     4261 gttccggcac cacagaggcc agatcgagat tcgctttcca gtaagcttcc agcgtaccca
     4321 catcgcgcca gtacggctcg gcatccgggt cggattgtac gcaagagagc gggaacgggt
     4381 gcgcataggc cagaccggct tcggtgatct tgggaatcaa atctttgcca aagtcgtggc
     4441 tggagttctc atcacgatcg tcttcttcca gcagttcata cagatagtcg gcgtcaaaga
     4501 cgtagatacc catactcgcc agagatttgc tcggatcgtt cggcattgac ggtgggttag
     4561 caggtttttc aacgaattcg ataattttat cgttctcatc aaccgccata acgccaaatg
     4621 cggaggcttc ttcaatcggt actggcatac aagcaacggt gcaacgtgcg cctttttcga
     4681 cgtgatcgat aagcatacgc gagtagtctt gcttgtagat atggtcgccc gccaggatca
     4741 ccacgtattc cgctttataa cggcggataa tgtcgaggtt ttgggtgacc gcatctgcgg
     4801 tgccgcgata ccagttttcc cctttcattc tctgctgtgc tggcagcaga tcgacaaact
     4861 cgttcatttc ttcattgaag aatgaccagc cgcgctgaat gtgctgcacc agagtgtggg
     4921 actggtactg ggtgatcacg cccatacgac ggatcccaga gttgatgcag ttagacagcg
     4981 caaagtcaat aatgcggaac ttaccgccga agtgtacggc cggttttgct cgcttattgg
     5041 ttaaatcctt caggcgggta ccacgtcctc ccgccagtat cagggcaaca gatttcaatg
     5101 gcagctggcg cgccaacatt aagtgatcgt tcttctctaa actaaccatg actaactcct
     5161 tttttatcat ctctggaaca cacacaatcc gtgtgcaggt ccctgccaga caaccgtaat
     5221 cactgggtta tcctctccag cgaatggggg aatggcgtgc cactccccag caggtaaaac
     5281 aatctctgtt acctcaagcg tggcgttaat tgcgatcaaa aagcgatccg agagcagaat
     5341 ttgcagctgt ttcggcccgt tttgccactc atccgtgctt aaaggttgag catatcgatt
     5401 tagccaacgg acattgccgt caccttcttc ccaccagcga ttctccacca aagcgggaat
     5461 gcgcttgcgc agatggatta acgcggcggt aaatgcggtt aaaccactgc ttgcctgcga
     5521 ccagtccaac cagtttaatt ggttatcctg acagtaggca ttgttattgc catgctggct
     5581 gtgaccatgt tcgtcaccgg ccagtaacat cggcgtgccc tgggagagca acaacgttgt
     5641 taacagggca tgaatgctgt cgcgccgccg ttcaaccagg tcaagagtac cgcctaaccc
     5701 ttctttacca tgattgttac tgtaattgtt gttggtcccg tcgcgatttt cttctccgtt
     5761 tgcttcattg tgtttatggt tgaagcaaac gcagtcgcga agcgtaaaac cgtcatgcgc
     5821 ggtgacgaga ttaatcgcgg cactcggcag acgaccatta cgtttaaaaa catcgctgga
     5881 ggcagcaaaa cgcccggcaa acgcccccag aggcaaatca taatgtagcc agaaacgacg
     5941 ggcagcatcg cggaaatgat cgttccactc ggcaaacagc ggcgggaaat ttcccacctg
     6001 ataaccacta ggagcgatat cccacggttc agcaattaac ttcacctgcg agagcaccgg
     6061 gcagttctgg atagcggtaa acaacggcgc atcctgacgg aactctggcg tacggcccat
     6121 gactgccgcc agatcaaagc ggaaaccatc gacgtggcag gtttctaccc aataacgcag
     6181 gcaggcgctg gcataatcca ccaccgccgg atgactcaaa ttgagcgtgt tgccgcaacc
     6241 ggtccagttg tgataatcgc cgtcttctct tatccaataa tagctacggt tatcgatccc
     6301 acgcagcgag aataacgggc cgtcgaggtc cagttccgca ctatggttga gcacgatatc
     6361 aagaatgact tcgatacccg ctttatgcag tgctttgatt gcatcgcgaa actcatccag
     6421 cgccgtttct ggcgagcagg catacgccgg atgcagcgca aacatcgcca ccgggttgta
     6481 accccagtag ttacttagcc ccatgcgttg cagacgtggt tcactggcaa actgcgccac
     6541 tggcagcagt tccagcgcgg taatgcccaa ttgtttcaaa tagttgatca tcaccggatg
     6601 cccgagggct ttataagtgc cacggatctc gaccgggatc tccgggtgca agtacgttaa
     6661 tcctttgaca tgggcttcat aaatgatggt gctgccccac ggcgtgcgcg gcggggcgtc
     6721 atcttcccag tcatagtgat caaccaccac tacgcatttc ggcgcaatgg cggcgttgtc
     6781 gcgatagtca ggttcattat gaccggcgtg cagcagcggg ttatctttaa actccccgtc
     6841 aatttgccgc gcgcaaggat caatcaacaa cttcgccggg ttaaagcgat gcccctcggc
     6901 gggttgccag gggccatgaa cgcgataacc ataacgcaaa cccgggcgcg catccggcag
     6961 ataaccgtgc caaatgtcgc cactgttccc tggcaagtca tagcgatgtt cctggccatt
     7021 ggcgtcaaag acacacagtt ctacccgctc ggcatgagcg gagaaaagtg tgaagttgac
     7081 gccctgaccg tcgtaatgcg cgccgagggg agcgggtttg ccaatggcga gttgtgtcat
     7141 tctgcctccc gaaccagcca gatagtggcc agcggtggta gcgtcaggct tagtgaatgc
     7201 tgacgaccgt ggctggcaat ctcatcgctg tgtaccgtgc cgccattgcc tgcattactg
     7261 ccgtgatagt gtatggaatc ggtattgagg atttcacgcc atttacctgg ctgattaatg
     7321 ccgaagcgat aatcatgacg cggcaccggc gtaaagttac tggcaacgat aatttcgtta
     7381 ccctctttat cgcgacgcac aaagatcagc accgagcgtt ctttgtcatc caccaccagc
     7441 cattcaaagc cgtacgggtc aaaatccagt tcatgcattg ctttatggtg gcggtaggtg
     7501 aggttcagat cgcgcaccag acgctggaca ccgtggtgcc agttatcgcc gccttccaac
     7561 agatgccagt cgaggctggc gtcatggttc cactcgcggc cctgggcaaa ttcgttcccc
     7621 atgaacagca gtttcttgcc cggaaatgcc cacatccagc catagtaagc gcgcaggtta
     7681 gcgaatttct gccatgcgtc gcccggcatg cggtcgagaa tcgatttttt accgtggacc
     7741 acttcatcat gcgacaacgg caggacgaag ttttcagtgt agttgtagag catcccgaag
     7801 gtcagtttat cgtgatgata ctgacgataa atcgggtcga gcttcatgta gtccagggtg
     7861 tcatgcatcc agccgaggtt ccacttgtac cagaagccca gaccgcccat atcctgcgga
     7921 cgagaaacgc cagggaaatc ggtagactcc tcagccattg tcaccgcacc ggaaacctgc
     7981 tcaccaagaa tacggttggt attacgcaag aattcaatcg cttcaagatt ctcgcgcccg
     8041 ccaaattcgt tcgggatcca ctccccctct ttacggctgt agtcacgata aatcatcgac
     8101 gccaccgcat cgacgcgcag cgcatcaata ccaaaacgtt caatccagta aagcgcgtta
     8161 ccgacgagga agttactgac ttcacggcga ccatagttgt agatcagcgt gttccagtcc
     8221 tgatgatagc cttcacgcgg atcgctgtgt tcatacaagt tcgtgccatc aaattcggca
     8281 agcgcaaagt catcagtcgg gaagtggcct ggcacccagt cgagaatcac gttcagacca
     8341 gctgcgtgtg cggcatcaat gaaataacgg aagtcgtcgc gagtaccaaa acggcgggtt
     8401 ggtgcataca ggccggttgg ctgataaccc caactgccat cgaagggatg ctcgttaatg
     8461 ggcagtagtt cgaggtgggt aaagcccatc catttagcat aaggcaccag ttgatcggcc
     8521 agctcgcggt agctcaacca gaaattgttg tcggtgtggc gacgccagga acccaggtga
     8581 acttcataaa tagagattgg cgcatcaaac tgattcgctt ttttgcgctc ttcagtctgt
     8641 acaacctttt ccggcagccc gcaaataaga gacgcggttt ccgggcgcat ttgcgcttcg
     8701 aaggcataag ggtcggactt cagacgcaag ttgccattgg catcaatcat ctcgtattta
     8761 tagagctgac cgttatgcgc cccagggata aacagttccc agatgccgct ctctttacgc
     8821 aggcgcatcg ggtgacggcg accgtcccag tagttgaatt gcccaaccac cgagacccga
     8881 cgggcgtttg gagcccagac agagaaacgc gtacctgtga cgccatccat agtatctgca
     8941 tgcgcgccta aggtttcata cgggcgcagg tgagtacctt cagataatag ccaggcatcc
     9001 atttcctgga ttagcggacc aaaacggtaa ggatcatcaa tcaggttttg ctgaccatgc
     9061 cagacaacag ccaactgata gcggaaaaaa ttcttacgtc gcggaatgac gccgctaaag
     9121 aatccccgtg agtcgagaca ctccagtttt gcgagtttgc gcccggtttt cggttcaatc
     9181 acccacacat cggtagcgtc gggtaaaagg gcacggactt ccagtcccgc ggtggtttta
     9241 tgcattccca gtacggaaaa aggatccgca aaatggcctg caattagcgc gttaatcacg
     9301 tctctatcga tacgatcgga catgcttgtc ttcctgtttt attgtgtcac cccatccaat
     9361 tggatttttg acttctggtt gtgacatttt tttgacctga acggcgcagc actctgtgca
     9421 tcctctctgc gtcgtcctca cttcaggtaa ggctgtgaat actcatgtat tcagccaccc
     9481 ttaaagaata gccaatgctc tatttaactc ccggtaaatc atgaaacatc tgcgcttact
     9541 cctgtattac gcactaacag gggcggcatc gcgccccaga tttaatgaat aaagattacg
     9601 ccagttgacg aagcatccga cgcagcggct ccgcggcccc ccacagcagc tggtcgccca
     9661 cggtaaaggc tgacaggaac tctggtccca tattcagctt acgcaggcgg cctaccggcg
     9721 tggtcagcgt gccggtaacg gcagctgggg ttagctcacg catagtgatt tcccgatcgt
     9781 tcggaacgac tttcgcccac ggattgtgcg cagccagcag ttcttcgacg gtcggaatag
     9841 acacatcttt tttcaattta atagtgaatg cctggctgtg gcagcgcaat gccccgacac
     9901 gcacacataa accatctacc ggaattacgg aagatgtgtt gaggatcttg ttggtttccg
     9961 cctgcccttt ccactcttcg cggctctgac cgttatcgag ctgtttgtcg atccacggaa
    10021 tcaggctacc cgccagcggc acgccaaagt tatccaccgg cagctcaccg ctacgggtta
    10081 aggttgtgac tttgcgttcg atatcgagaa tagcagagga cggggtcgcg agttcatctg
    10141 ccacatggcc atacagatgg cccatctggg ttaataactc acgcatatgt cgcgcaccac
    10201 cgccggaagc ggcctggtag gttgcaacgg acacccaatc aacaagatca ttggcgaata
    10261 aaccacccaa cgacatcaac atcaggctta cggtacagtt accgccaaca aaagtcctga
    10321 tgccattatt taatccgtcg gtaatgacgt cctgattgac ggggtcaaga atgatgatgg
    10381 cgtcatcttt catgcgcaga gacgatgctg cgtcaatcca gtaaccttgc catccgcttt
    10441 cacgaagctt tggatagatt tcgttggtat aatcgccgcc ctgacaggtc acaatgatat
    10501 cgagggcctt tagcgcctct agatcaaagg catcctgaag tgtgccagtg gttccgccaa
    10561 aagacggcgc agcctggcca agctgagaag tagaaaagaa gacagggcga atggcgtcga
    10621 agtcgcgctc ttcaaccatg cgttgcatga gaacggagcc gaccataccg cgccagccga
    10681 taaaaccaac atttttcata agcgtttttt cctgcaaaga tgtgtgctgt ataaatgtgc
    10741 cggtctcctc ttggcacatc tttcaccata caaaaagctg ccaaagtcgc aagtgaaatt
    10801 aatcaatgat agcgaagcca tcagtaatgc gacttatcct gctttgttag cacgcagaaa
    10861 gtccgcggca attatcaggg aatttgagtt atgaatgaaa tcatttctgc agcagtttta
    10921 ttgatcctga ttatggatcc gctcggaaac ctgcctattt tcatgtccgt actgaaacat
    10981 actgaaccga aaagacggcg ggcaatcatg gtgcgagagt tgcttattgc tctcctggtg
    11041 atgctggtgt tcctgtttgc gggtgagaaa attctggcat ttctcagcct gcgagcagaa
    11101 accgtctcca tttctggcgg catcattctg tttctaatcg ccattaagat gattttcccc
    11161 agcgcttcag gaaatagcag cgggcttccg gcaggtgaag agccatttat cgtgccgttg
    11221 gcaattccgt tagtcgccgg gccgactatt ctcgccacgc tgatgttgtt gtctcatcag
    11281 tacccgaatc agatgggaca tctggtgatt gctctgctgc tggcctgggg cggcaccttt
    11341 gtcatcctgc tacagtcttc gctattttta cgtctgctgg gcgagaaagg ggtgaacgca
    11401 cttgaacgcc tgatgggatt gattctggtg atgatggcaa ctcagatgtt cctcgacggc
    11461 attcgaatgt ggatgaaggg gtaacgagga gagtttttcg aaaagagccc ggtagcgtca
    11521 caactaccgg gcgaacaaac ttaactcaac agctggaacg caatcatccc aacgatggca
    11581 ccaacagtgc cgaggatggt ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc
    11641 gcgccggtaa atttaccgaa caaccagaaa ccagcgtcgt taacgtggct gacaacaatc
    11701 gaaccaccag cgatacaaat cgacagtgcc gccatttgcg caccagagta gttcagttgt
    11761 tcaatgactg gcatcaccag tcccaccgcc gttaaacagg ctacggtggc agccccctga
    11821 atgatgcgca ctgcagcagc cagcacgaag caggtgatag caatcggcag gcccatgccg
    11881 gttaacgctt cgcccagtgc cggacctacg ccagagtcaa ccagcacctg tttgaacacg
    11941 ccgcctgcac caatcaccag cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc
    12001 tccatcactt tgtcttttgg catgccctga cgcattgcca ggccataaat cgctaccaga
    12061 caagcaacca gaatcgcggt aaacggatga ccaataaact cgaaccattc gtaagcggta
    12121 gagccttctg gcacaaaacg cgcggcaatg gttttcagcc ccaccagcac cagcggcaac
    12181 aggatcagcg acaggctgaa tccgaaagat ggcattttac cttcgccgag atgcggttcg
    12241 ctgatgtcgt caggaatatg cagctcaacg taacggctga tgaagttacc ccacagcggc
    12301 ccggcaataa tcattcccgg aattgccgca cacaggccaa tcaggatcat ccagccaaag
    12361 tcggcattca tctgcgatgc cagcagcatt ggcgctggcc caggcaccag gaacgctgcc
    12421 gcggccgcca cgcctgcgaa taatgggatt accagcttca ccaggttcgt accggtgtgg
    12481 cgcgccattg agaaagcaac gctaatcagc agaacaatcg ccacttcaaa gaacagcggt
    12541 agcgcacaga ccagccccgc aaggccgatg gcataatgcg cgcggctgtg accgaaggat
    12601 ttgagcattt tgacggcaat ctgatcgact gcgccggttt catataagat cttgccaaac
    12661 atagctccca gggcgacaac caccgccagg aagccgaggg tgcctcccat ccctttttcc
    12721 atcgtcgctg cgattttatc gagcggcata ccggaaaaaa ggccagcccc catggacacc
    12781 accatcaaag ccaggaaagc gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa
    12841 acagacccta ctgctgttaa aacaagcgtt aatgtagtca ctacttattt gcctttttta
    12901 ataacctcaa tggtgcttgc cacaacacct tccagcggtt gatcgatatc caccaccagt
    12961 acatcggttt cgtccgcacc cggctcctgc agcgtttcaa actgcgtcac caacatttgg
    13021 gttttaaaga aatggccttt gcgcgctttc aggcgacttt caatcacatc aaaatcgcct
    13081 ttcaggtaga tgaaagagag attcggatta ccttcacgca gcaagtcgcg atagtgcttt
    13141 ttcagtgcag aacaggcgat cagcgacact ttattggtgc gctgcatagc aaacgcggag
    13201 tcgttcagcg cctgcaacca gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc
    13261 attttttcga tattgcgccg tggatggagg aaatcgccat caagaaacgc ggcatgaagt
    13321 tgatgcgcca cttcactggc gaccgcagat ttgccgctgc ccgatacgcc catcaagacg
    13381 taaatgtggt gatcatggtt agtcgtgctc aaagtggtgc ccccacaata caagaattaa
    13441 aaatgttacg ggtaactgtt atcggcaaca ttgtccagcc ggacaatatc agaagcaata
    13501 tccatccgtg ccttaagtgt ataagtgtga gctacttcaa atttgtgggc ttaaatagat
    13561 ccgcccggtg acaaggtgaa acctaaatct aacattttcg gtgtcacaga ttcgccacga
    13621 atacgcgcca gcaggcgttc agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg
    13681 ctcgcaagtc gtggctccat cacctgacca atgtcatgac cgtggaaacc ggcaatcgcc
    13741 atatcgtcag gaacttttaa ccccagacgc tgacattcaa acgccgcgcc gaccgccaga
    13801 tcatcattag tacagaacac gccatccagc tgcggatatt cccgccgcgc ctggcgaatc
    13861 agttcaatac cggaagagta agaagaagat tgctcaacca tcacgctata tggcaccagg
    13921 cctgcatcca gcatcgcctg ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga
    13981 cgtgcgccga gataggcaat gtggcgatgc ccacgagcaa taatggcggt agtcatctgg
    14041 cgtgccgctt caaagttatc aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc
    14101 atcaactcca ccaccgggat ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg
    14161 gtacgttcgg tgaggatcag gccgtcgata ttccaggaga gcatggattc gagacgttct
    14221 tgctccattt ccggtttata accgtagtgc gccagcatgg tctgataacc gtgcgcgtcg
    14281 gtgacgcttt cgattccgcg taatacttcc gcgaaaacct ggttggtgag agaaggtaac
    14341 aggacgccaa tcgcccggct ggtagcgtta gagaggatat cgggcgcacg attgggaata
    14401 tagcccagtt catcaagagc ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc
    14461 gggttgcgta aaaaacggct gaccgtcatt ttggtcacgc ctacacggtc agccacatcc
    14521 tgaagtacgg gtcttttctt tttcatcgtc ctgaaggtac aaaagagata gattctctta
    14581 gtttaacacg gacgcatcag aaccttcccg gggaaaacag tgcttgttta caattattag
    14641 atatacacta aatcaattgc atcgcattgt gctaacgacg cggcaaaacc ccggaagcgt
    14701 gcatcagcac gcgaccggag cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc
    14761 cgttgagttt taaaccggcg gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg
    14821 gatggagatt gcctgctcat cccagattgc cagaccatcg ctggtcgagg ctttcacgcc
    14881 gttaatggta acattgcctt ttaccacctg aatccagacg cggcgttcag cggcaatctg
    14941 atgcaccgac tgctcatctt tcagcaacgc ccaacgatac agttccatat cctgatgcac
    15001 tttcagcgaa ccatctcgcg catccggcga gagcaccagc tgtttgccct gcacggcatc
    15061 gaagcgacgc tgttcataac gcggcgtaat gccattttct tcaggcatga tccagatctg
    15121 atacagatgc agacgctcgg tgctgcttgg gttgtactct gagtgacgaa tacccgtacc
    15181 cgcactcata atctggaact cacccgccgg aacctgctct ttattgccca tgctgtcctg
    15241 atgctcaaca gtaccttcca gcacgtaggt caaaatttcc atatctttat gcgggtgagt
    15301 gccgaagccc tgccctactt caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc
    15361 cataaagttc ggatcgtaat agttggcaca agagaaagta tgccaggagt ccagccagcc
    15421 atgatttgca tgaccacgtt catttgcttt gcgtaagtag atcatttgta ttcgccccct
    15481 gaatgatttc gatgggctta gtgtggaccc gatctgcctg ggatgataga gggtgaaaat
    15541 tgccccctct gttcaaaaaa attgaactat tcgaggggcc atcaaactta attcgcgaga
    15601 gtcactgtgg agggagaggc ttgctcaaat ccgcgttcaa ggatttccag attggtaaga
    15661 acttcagatt ccttgacgta atttggcgca ccgtgggtga tggtttgata caacgcatca
    15721 taaacacgcc cgtaatcgcc catctccggc ttcatctctt ctctgaccgt cacaccctcg
    15781 tcattgacat actccagcac accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc
    15841 ataatattag ccttcaggct ggtttcctgc tggtcgatac cgtatttaat aaacgaacct
    15901 ttcttaccgt gaacgataaa tttcggataa tcgattttca ccagatggct ggttttgacg
    15961 atggctttca ggtcgccata aaacagttgc gcttcaaagg tgtcgtcagg attggcttta
    16021 ttacgcaggc tgcggatgtc ataagcgacg tgatccgggc gaccgaacag agaaataatc
    16081 tggtccatcg tatgcacacc aaggccatag aacgcgccat cctgcggcag tccaggtttg
    16141 gtttctgcca ccgggcggta atagtcaaaa tggctttcca cttcgacaat ctctcccagc
    16201 ttgccgcttt caatcgcttt tttcgccgtc aggaagcagg aatcaaagcg acgattctga
    16261 tatggcgtga cggtcagccc tttgcttttc gccagtgcaa acagctcttt cgcctgcgca
    16321 agtgtcggag tgaacgtttt ttcgaccagc acatttttcc cggcttccag cgcgcgcttc
    16381 gcgtactcga agtggctgtc cgcatgggtg cagacaacaa ccaacttaac atcgggatcg
    16441 tttagcactt cgtcgagatc gctggtgaaa tggatatgag agtaaatggg agcctgttct
    16501 tccggcttcg catggcgacg aaaaatatgc gcgacatgcc agctatcctt gcggttaagt
    16561 acatacggca gatggtaacg ggtggtgctt ttgccgaagc caataaaggc gcagttgatg
    16621 accatgattt cgtccttttt aaggtggtta tttacacctt agcgcaaagc ggacgtggtt
    16681 cctactggag tgcgcataac gaacacaagc actcccgtgg ataaattgag aacgaaagat
    16741 caaaaaaaaa gccagcaccc ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg
    16801 tgctttcagg ttctccgcga gggtcttcct gatcgcgata caataataat cattctcatt
    16861 cgcacttgtc caacactttt tgcaaaaaaa tgcatttgac tcgcatttga aagtcaatga
    16921 tgttgaaagg gacatttacc ccaaagagga caaaggaatg agtgagatag taatacgcca
    16981 cgcagaaaca cgggattacg aggccatcag gcagattcac gcccagccgg aggtgtattg
    17041 caacacacta caggtgcctc atccttccga tcatatgtgg caggagcgac tcgccgatcg
    17101 tcccggcatc aagcaactcg tcgcctgtat tgatggagac gtcgtgggcc atctcaccat
    17161 tgacgtgcaa cagcgcccac gccgcagtca tgttgccgat tttggtatct gtgtcgactc
    17221 tcgttggaag aaccgcggcg tcgccagcgc cctgatgcga gagatgattg aaatgtgcga
    17281 caactggtta cgggtagatc gcattgaact aaccgtgttt gtcgataacg cgccagcaat
    17341 taaggtctat aaaaaattcg gctttgagat tgaagggacc ggtaagaagt acgcattgcg
    17401 taatggtgaa tatgtcgatg cgtattatat ggcgcgggtg aaatagagaa tttgcaatct
    17461 aagtttgtca tttaagccct cacctaccgg gagggcttaa gatattaata ccccgccgtt
    17521 aaatcatcca ccgagcgcgg gtcggatgcg ccgtacaact caccgtccgg cccaaccata
    17581 atgctttgtg tgctgcccat cgcctctttc agcgccactt tctgaccttt tgcttccagc
    17641 agcttgagcg tatccgggct aaaccctttt tcgacacgca gctcgtccgg caaccactga
    17701 tggtggaaac gcggcgcatt ggtcgcttcg gcgacgttca tgccataatc gatgctattc
    17761 accaccattt gcagcaccgt ggtgataatc cggctaccgc ctgggctacc ggtaaccagc
    17821 caggttttac cgtctttcac cacaatggtc ggcgacatcg acgacagcgg gcgtttgttc
    17881 ggcccgacgg cgttggcatc accgcccacc agcccgtaaa cgttcggtac gcccggtttg
    17941 gcggagaaat catccatctg gttattaagc agaataccgc tctcacccgc gacaataccc
    18001 gtaccgaagg tggtgttcag cgtataggtc accgccaccg cgttaccgtc tttatccact
    18061 acagagtaat gggtagtttg attactctca taaggcgcaa gcttgccagg gcgaatctca
    18121 ctggacggct tcgctttttt gatatcaatt tgatcggcaa tggatttggc ataggcttta
    18181 ttggtcagtg cctgccacgg gactttgaca aaatccgggt cgccaagata ttccgagcgg
    18241 tcggcgtagg cgtatttctc cgcttctgcc atgatttgca tcgcatcggc gctgccaaag
    18301 ccgtatttct tcatatcgaa gttttccaga atattgagga tttgtacgat atggatcccg
    18361 ccggaggatg gcggtggcat ggagtaaacc tgatacccgc gataatcgcc gcttatcgga
    18421 gtgcgttcga ccgctttata ggccgctaaa tcttctttag tgatcaagcc accgtttttc
    18481 tgcatctcct gggcgatctg ttccgcaatc gtgcctttat agaattcgtc cgggccgttt
    18541 tcagcaatca tctccaggct ctttgccagg ttcgcctgca ccagcgtgtc gccctttttc
    18601 agcggctcgc cctctttcca gaagatagct ttactgtttt cgtgattcgg caacacttcg
    18661 ctaccgtagg ttttgagatc gtcagccagc gcgtcgttaa cgataaaacc atcgcgtgcc
    18721 agtttaaacg cgggctgcac gactttgttc agcggcatgg tgccgtattt atccagcgcc
    18781 agcgagaaac ctgctaccgt acccggtgtg ccggaagcca gatgcgaagt gagtgatttt
    18841 ttgctgtccg ggttgccctg atcatcgagg aacatatcgc gggtcgcttc tttaacgcgc
    18901 tcggctattt gctgcttgcc catgccgcga attttcagcc cccacgccat gttttcttcg
    18961 acgctcatat gcggataaag cgcgtaattc tggaacacca tcgcaatccc gcgatctttc
    19021 ggctccattt cggtcacgcg ctggtcgttg atccagatat cgccttcggt caccctctcc
    19081 agcccggcaa ccatacgcag cagcgtcgat ttcccgcagc cagacggccc gaccatcacg
    19141 ataaattcgc catccgccac atcaagggtc agcggtttaa tgacctaggt tttgccatcc
    19201 cagcttttgg ttactgcctg taatttcagt cctgccatct tatttctcac tatcgaccag
    19261 gccgcgcacg aaggcacgct gcatcactaa aacaatcact actggtggga tcagggttaa
    19321 caacatcgcc gccatcactg agttccattc cgtggtgcct tcgccagtgg cgatcatccc
    19381 tttgatccct gccacggtgg tgccgagatc cacgtcggta ataatcaaca acggccacaa
    19441 atactggttc cagccgtaaa taaaggtgat cacaaacagc gccgccagat tagttttgga
    19501 gagcggaaaa acgatgtcgc aaaagaagcg cattggtgac gcaccgtcga tccgcgccgc
    19561 ttccaccagt tcatccggca gcgtcataaa gaactggcga aacagaaacg tggcggtcgc
    19621 cgaggccatc agcggcagcg ttaaaccggc gtagctgtcg agcatcttca ggttggcgat
    19681 gacttccacc gtcgggaaga tacgtacttc aaccggcagc atcagggtga taaaaatcat
    19741 ccagaagaag aggttacgta gcggaaaacg aaaccagaca atggcaaatg ccgagagcat
    19801 cgagacagta attttgccga gcgtaatgct gaacgccatc acaaagctgt taagcaacat
    19861 ccgccagaac ggcgcgctat ttgtgcctac cccgttcacc cagatgttgt ggatgttttc
    19921 cagcagatgc gtgccgggga tgagcgtcat cggcgcggcg tagacggcct gtttatccag
    19981 cgtcgccgcg acaaacgcca cgtacagcgg gaagaggatc accgcgatcc cgaggatcag
    20041 catggtatgg ctgaatatcg tcagccacgg acggttctca atcattggta acgcaccttg
    20101 ctttcaacat agcggaactg caccaccgtc agcacgatga cgaggaacat cagcaccatc
    20161 gactgtgcgg cagacgaagc cagatccagt ccggtaaaac cttcgcggta gatcttataa
    20221 atcagcgtcg tggtggcctg aaccggcccg ccggacgtgg cggcgtcgat caccgggaag
    20281 gtgtcgaaga aggcatacac caggttcact accagcagga agaaactcac cggggcgata
    20341 agcggcagcg caatcttaaa gaagcggcga atcggccctg caccgtcgat ggctgcggct
    20401 tcgatcaacg aacggggaat ggattgcagc gcggcataga agaacagaaa gttgtagctg
    20461 atttgcttcc acaccgaggc aaacaccacc agaaacatcg cctgaccgct attttgcgcg
    20521 tggttccagt cgtagccgaa ctcagcgaga aaatgggtga tcagcccgcg accggggtta
    20581 aacaggaaga tccacaatac ggcggcaacg gcgggagcca cggcatacgg cagcaacatt
    20641 aaggtttggt agaaacgact gccgcgcacg atgtactcca ccagcgccgc aaagaacagc
    20701 gataccagca aaccgctgac ggtgacaaag gtgctgaatt ttatcgtcgt ccagaaggag
    20761 tcgagataat agctgtcatg aaacagcgtg acgaagttat ccagaccgac aaactggctg
    20821 gagaaaccaa acggatcgac gctttgtagc gagtaccaca acgcttcgcc cgcaggccag
    20881 ataaaaaaga taacggtgat gatgagctgc ggcgcgacca gcagataagg cagccagcgc
    20941 gagcggaaca ccggacggga tgatgacatt acggttaatt cctgaactgt gccggatgcg
    21001 cttcgcttat ccggcctaca cgcgatgcca ggtcagataa gcgaaacgca tccggcatta
    21061 cactgattaa gacttcgtcg atttctcaaa gcggcgcagt aactggttcc cacgctcaac
    21121 tgcggtatcc agcgcctgct gtggcgtctt cttaccggtc cacacgctct caagctcttc
    21181 atccacaatc acgcggatct gcggcatgtt gcccagacga agccctttgg tgaacggcaa
    21241 cggcggcttg ttcagcatct gacgcgtcgc ggtatccgca cctgggtttt tctcgtaaaa
    21301 gccctgctca cgggtcaggt catacgccgc tttggtaatt ggcagataac cggttttctg
    21361 atgccactca gcagcgtttt ctggcttcgc gaggaagtcg agaaacttcg ccacgccggt
    21421 atacgtttct ttatctttgc cctgcatcac ccacaggctg gctccgccga taatggcgtt
    21481 ttgtggcgca tctttcgcat cggcgtcgta aggcatcata cctacgccgt agttaaattt
    21541 ggcgtactcg cgaatgttgg caagagaacc ggaagaggcg gtggtcatcg cgcaatcacc
    21601 gttatagaac ttctcggtgg attcatcctt acgaccgacg tagctgaagt cgcccttctt
    21661 gttcatctcc tcgagcatgg cgatgtgttt cacctgctcc ggcttgttga actccagcac
    21721 cgcgtccgtg ccgtcaaagc cgttgttttt gctggcaaac ggcagaccgt tccaggcgct
    21781 aaagttttcc agttggatcc agccctgcca gccgctggcg tagccgcact taataccgga
    21841 ggctttcagt tttgcggaat agtccgccag atcctgccag gttttcggcg gctgttccgg
    21901 gtctaacccc gctttcttga aggcgtcttt gttgtaatag agaacggaag tcgagctgtt
    21961 gaacggctgg gagagtaagt ggccggtttt gctgtcggag tagtaaccgg aaaccgtcgg
    22021 cacaaactgc gactcatcga actgaatccc tgcctcttta aacacgtcat acaccggttt
    22081 aatggctttc gacgccatca tggtggcggt gccaacttca taaacctgca aaatagccgg
    22141 agcgttgccg gtacgaaata cggcaatccc cgcgcttaaa ttctgttcgt agttgccttt
    22201 ataggtcggt acaattttgt aatccggatt ttcggcgtta aaacgttggg ccagagaatc
    22261 cacctcttta cccagttccc cttccataga atgccagaac ggaatggtcg tcactgcctg
    22321 tgcattcccc attaacgcca gtccgagcgc cagtgctgaa gctgtataat gtaacggttt
    22381 catcgtttat ctctcttgtt gtaccgaatg cgcgaattca cgcgttttat gctcgcgggg
    22441 taacatgaca tgctcgaatt acagaaaaat aacttttttg ttacatttgt aagatagtaa
    22501 ggtgtcagaa agatgacaag gcggtgacgg cgtgggtgag ggaaaatggg agatggggca
    22561 cggataagcg gaaaaataga aggtctgaat caaactctac agattactca tcgtttcatg
    22621 ccggatgcgg cgtaaacgcc ttatccggcc tacaagatcg tgcaaattca atatattgca
    22681 actcacccag taggcctgat aagcgcagcg catcaggcaa ttttgcattt gtcacctgtc
    22741 tcaaaggagt cttttgactc cttatcaatc aacgtgttat tacccgccta aatacgcact
    22801 tctcactgct tcgttcgcca gcagcgcgtc gccggtatcg gagagcacca catggccgtt
    22861 ttccagcacg tagccgcgat ccgccagctt tagcgcctgg ttggcgttct gctcgacgag
    22921 gaagatagtc atcccctgct cgcgcagctg ctcgatggtg tcgaaaattt gctggatgat
    22981 aatcggcgca agaccgagcg atggctcatc aagtaacaac aaacgcggat tgctcatcag
    23041 cgcgcgacca atcgccagca tctgctgttc accgccggac atggtgcccg cccgctgaac
    23101 acggcgctca tgcagacgtg gaaacagttc atacacccac tttatgcgct cctgaaactg
    23161 gtcacgttcg gcaaagaagc cgcccatcgc caggttctct tccaccgtca tccgcgagaa
    23221 gacgcgacgc ccttccggga caatcgctac cgcttcgcgc atgattttcg ccgtctgcca
    23281 gtcggtaatg tctttatcat caaacacaat tcgcccgctg gtggcgcgcg gatcgccgca
    23341 taacgtgccg agcaaggtgg ttttccccgc cccgttcgcg ccaatgagag tgacaatctc
    23401 gccctgattg atatgcaggc tcacctcatg cagcgcctgg attttgccgt agtgggcgct
    23461 gactttgtca aaggacaaca tgactttttc catcttatgc ctcacctaaa taggcacgga
    23521 tcacgtccgg gttattacgg atttgctccg gcgtaccgtt tgccagtggc gtcccctgat
    23581 tgaccacata aattcggtct gaaatcccca tcaccagctt catatcgtgt tcaatcaaca
    23641 aaatagtggt gttgtgatga ttgcgcagtt cggcaatcag ctcatccagc tctttggttt
    23701 cttttgggtt aagacctgcc gccggttcgt cgagcattaa aatctccggc tgcgtcacca
    23761 tgcagcgggc aatctccaga cggcgttggt caccataggc caggttactc gcctgacggt
    23821 tggcgtgttc cagcaaacca atgcgctcaa gccaggtggc ggcgcggtcg agcgcttcgc
    23881 tctgggcgcg acggaaagaa ggcgttttca acaggccaga gaacagcccg gttttcagtt
    23941 gctgatgctg cgccaccagc aggttttcaa ttaccgtcat ttcacggaac agacgcacat
    24001 gctggaaggt gcgcaccacg cccatgcgag caatttgctg ccccggtaaa ccttcaagat
    24061 gctgatcgcg cagtaaaatg gtgccgccgg tgggtttgta gaaaccggtc aggcagttaa
    24121 aaaccgtggt ttttccggca ccgttagggc cgattaacga gacgatttcc tgcgggtaca
    24181 gttcaagatt gacgttgttc accgccagca ggccgccgaa gcgcatcatc agaccgttaa
    24241 cagataataa tggctgactc atgcctgctc tcctttcgct gcgccgtttt tcagcttcag
    24301 ttgcgggcgc gtcatgggca gcaaaccctg cggacgccag atcatcatca gcaccatcaa
    24361 accaccgagc attaacatgc tgtattcgtt gaaatcacgc atcaattcgc gcgacaccac
    24421 cagcagaaca gccgccagaa tcactgcaaa ttgcgagccc ataccgccga gcaccactat
    24481 cgccagcaca aaggccgatt cagcaaaggt gaaggattcc gggctgacaa agccctgacg
    24541 cgccgcaaac agcgttccgg caaaaccggc aaatgcggcg ctgatggtaa aagcagtcag
    24601 cttgatacgg cgcgggctta agcccagcga acgacaggcg atctcgtctt cacgcaacgc
    24661 ttcccacgcg cggcccagcg gcatccgcag caggcggtta atgacaaaca ggcttaacac
    24721 caccagcagc aacgccacca ggtagaggaa gataacgcga tcggacggat cgtatttcag
    24781 gccaaagaaa ttactgaacg tgtcccagcc gccttcacga gcggtacggc tgaactcgag
    24841 tccgaagagt gtcggtttcg ggatctgact gattccgttc gggccgccgg tgatttcagt
    24901 gttattgagc agcaatatgc gcacaatttc gccgaaacca agggtaacga tcgccagata
    24961 gtcaccgcgc agacgcagca ccggaaaacc gagcaggaaa cccgccgccg ctgccatcag
    25021 tccggcaatc ggcaaacagg tccagaaccc cagaccgtaa tagtgattga gcagcgcaaa
    25081 agtgtaagcg ccgatggcgt aaaaaccgcc gtaccccagc accagcagac cagaaagacc
    25141 aacaaccaca ttcagcccga gaccgaggat aatgtagatc atggtcagag tggcgatatc
    25201 caccgtccca cgtgacacca taaacggcca cgccaccaca agcaccagca gcgccacgag
    25261 gaacagcttt tgcttcaccg tggacccatc aatcgcgggc agaataaact tcggcccgga
    25321 aacgcttttc aaccctttct ggaaagccgg tcgcaaaagc tggaagaaaa agaccaccgc
    25381 cgtgccgata aacatccact gccaacggat gtctgaagcc gtgtcgacca ccagtttggt
    25441 gccatccagc tccagttgta cgcccataaa gacgcccgcc aggacaaaga acatcgcggc
    25501 agagagcagt gccattgcaa tatgcatcgg tttcatactt tctctacctc cgggcgaccc
    25561 agaataccgg tcggcatcac cagcagcacc agaatcagca gggcgaatga gaccacatct
    25621 ttatattccg tactcagata ggcagaagag agcgcctccg caatccccag gatcagcccg
    25681 ccaatcatcg cccctggaat gctgccaatc ccaccgagca ccgcagcggt aaaggctttc
    25741 atcccggcca taaagccgat ataggggtta atgacgcagt agaactgacc gagcagtacg
    25801 ccagccactg ccgccatcgc cgcgccaata acaaaggtca aagcaatcac ccggtcggtg
    25861 ttgatgccaa gcaggctcgc cattttcaga tcttccgcgc aggcacgaca ggcgcgcccc
    25921 atacgggaat agcgaatgaa aatcgtcagc gccagcatgg cgaggaaggt gacaatccag
    25981 atcaccgcct gcatggtggt aatagaggca gagaagtttt cgctatggcc caccacccac
    26041 tgaccgttaa acaggctcgg cagcgccacg tcgcgcgaac cttcggtcag gctgacgtag
    26101 ttttgcagga agatggacat accgattgcg gaaataagcg caatcaggcg cttagagtta
    26161 cgcaccgggc ggtaagctac ccgttcgata ctccagccgt aggcgctggc gatgacgatt
    26221 gcgccgacga atcccgccgc caccagcagc cagccggtat caatgcccat catcatcagc
    26281 gcggcgatga tcataaatga gacgtagctg ccaatcatat aaacctcgcc gtgggcgaag
    26341 ttgatcatgc cgataatgcc gtaaaccatg gtgtagccga tggctatcag cgcgtaggta
    26401 ctgcccagcg tgacgccgtt aaacatctgc tgcaagaaat acaaaaactg ctcagacata
    26461 aggtaacctt tctaaacccg cccgcatttt acgggcggtg ggatgatcac ttggctgccg
    26521 tggatgaacc gtcggcgtgc cactggaaga caccaaaatc aaatccctta agatcgcctt
    26581 tttcatccca gttcagcggc ccaatcacgg tgtttgcacc gttagctttt aaatctttca
    26641 ccagcgccag cggctcatcg ctgccggtac gctcaagggc agttgccaga gattgcactg
    26701 ccgcgtaagt gatccagaca taaggcccgg acggatcttt cttgtctgct ttcagcgcat
    26761 caacgatgcc ctggtttgcc ggatcctggt catagcgttt tggcatagtg accaacatgc
    26821 cttcggcggc atcaccggca atgttcgaca gcgatgcgtt accgacacct tccggcccca
    26881 taaactgggt tttcaggcca acggaacggg cttggcgcag catctgcccc atttccgggt
    26941 agtaaccgcc gtagtaaacg aagtcgatat tttctttttt caggcgggcg atcagcgcgg
    27001 agaaatcttt ctccccggcg gtaataccgt cgaagaagac gacgttggcg ttagccgctt
    27061 tcagcccgtc ctgcaccgaa cgcgccagcc cttcgccata ctgttgtttg tcgtgaatga
    27121 tggcgatgcg ctggggcttc accgtctcaa gaatgtattt tgccgccgta ggcccctggg
    27181 aagagtccag cccggcagta cgcataatgt gttgataacc gcgttgggtc agctccgggt
    27241 tggtcgctcc cggcgagatc atcagaatac cttcgtcttc gtagatatcc gacgctggct
    27301 gggtagagga agaacacaga tgaccaataa cgtatttaat gccgtcgtta acgattttgt
    27361 tggcgaccgc aacggcttgt ttcgggtcgc aggcgtcgtc atattccacg ccaaccagct
    27421 tgtcgccctt aattccccct ttgacattaa tgtctttaat cgcctgacgc gcgccgttaa
    27481 attccatatc gccccactgg gcaatcgggc cggacatcgc gccgacaacg gcgactttaa
    27541 tatcgtcagc catagcggtg tgtgaaattg ccagtgcaat catccctgcg atgatagttt
    27601 tcgcattccg tttcatagtc aaaaatcccc attcgtgatg ttgtgttgct ttgtttttat
    27661 gtgttaacaa atcagactgt tcttttttta tactgcactg tttttgcctg tctgattaag
    27721 gggttagcgc agtattttgt gatgatagcg attaaaatcc ctatttttca gtcgattaag
    27781 aacagataat attctgaatt tattgataga taaacagaaa aaagtgcctt tgtcagcata
    27841 aaataacggc acaaagggcg gaataattca ctatcattca ggggattatg ctggacattt
    27901 ttcattctct aatgttttaa ctttgtaatt attgctgtta aaaaattaat cacctgccaa
    27961 aagaaataaa aaagagaaag cctccgatta aattatttcg ctacactggt tccacttttg
    28021 tgatttacac gggttaccca tgaagctgac catcattcga ttagaaaact ttagcgacca
    28081 ggaccggatt gacctgcaaa agatctggcc ggagtattcc ccttcctctt tacaggttga
    28141 cgataaccac cgtatctacg ccgcgcgttt taacgagcgt ctgcttgcgg ccgtgcgggt
    28201 aaccttaagc ggcaccgagg gagcactgga ttctctacgc gtgcgggaag tcacccgccg
    28261 tcgcggtgtg gggcaatatc tgctggaaga ggttttgcgt aacaatcctg gcgtttcatg
    28321 ctggtggatg gcggatgcag gcgtggaaga tcgcggtgtg atgacggcgt ttatgcaggc
    28381 actgggattt acggcacagc agggcggctg ggagaagcgt taatcgtcaa gttgatttca
    28441 aaagtgatga ccaacgagaa attgcctgat gcgctacgct tatcaggcct ataatgtgtg
    28501 ctgcaattta ctgatttctt tggttcttgt aggccggata aggcgtttac gccgcatccg
    28561 gcatgaagca acgtattcga tattagcaat ttggcggcaa cccaaagttg ccgattaaca
    28621 attacttcgc atcggtcgcc gtgccgttgg cgtgccagtc aaatacgccg aactcaaagc
    28681 cctttagatc gcctttctca tcccaagtca gtggccccat cacggtatcc acggagttcg
    28741 cttttaggta tttggcgatt tcagccggat cgtcagactg attcaggccc gcctgcaaag
    28801 attgcagcgc ggcgtaggtg gtccagacga atgcgccgct tgggtcctgt tttttcgctt
    28861 tgatcgcatc aacaatgggt ttgttcgccg gaacctgatc gtagttcttc ggcttggtca
    28921 ccagcagccc ttccgctgat tcgcccgcaa tgttagacag cgaaacgtta gccacacctt
    28981 ccggccccat aaactgagtt ttcagccctg ccgcgcgtgc ctgacgcagg atttgcccca
    29041 tttccgggtg ataaccgccg tagtaaacga agtcgatatt ctcttttttc agacgcgcca
    29101 ccagcgttga gaaatctttt tccccggcgg tgataccatc aaagaacacc acgttagcat
    29161 tgcctttctt caggccgtcc tgcaccgctc gtgccagacc ttcgccgtat tgctgtttgt
    29221 cgtgaacgat agcaatacgc tgcggtttca ctttctcaag aatatatttc gccgccgtcg
    29281 gcccctggtc ggagtccagg ccggtggtgc gcaggatcag ctgatagcca cgggcggtca
    29341 gctccggcgc ggttgccgct ggggtgatca ttaaaatgcc ttcgtcttcg tagatgtcag
    29401 acgcaggctg cgttgatgaa gaacagaggt gaccaatcac atatttaatg ccgtcgttaa
    29461 cgactttgtt cgccaccgca accgcctgtt tcgggtcaca ggcatcgtca tattttacga
    29521 tttgcagttt gttgccttta atgccgcctt tagcgttgat atccgcaacc gcctgctctg
    29581 cgccggtaaa ctcctggtca ccgtactgcg caaccggacc ggacattgca cccacgaccg
    29641 cgactttaat atcttctgcc agagccatat tgctgaatgc cagcgcgata catcctgcca
    29701 gtaacgcttt accctttatg ttcatcctga gaatccccat tcttctggtt attacgtgtg
    29761 ttgtgatgtt gttgttcagc actttatttc gttttatgca tgactacccg tgctttagca
    29821 gcatactctg ctaaaacata cccgattttt atgatattgg aatagctatt ttgacagttt
    29881 attaacaatc tgcgtgggga ttggcgtttt gccggagggg gaattgatta ttgtagaggc
    29941 ccaaaaaaca aaaaccccgg actctcatcc agggttctct gcttaatagc ggaaattacg
    30001 cttcaatagc ggcgcgaagt tttttcatcg cgttcttttc cagttggcgc acacgttcag
    30061 cggaaacacc gtaacggtca gccagttcct gcaacgtgga cttgttgtct tcgtccagcc
    30121 agcgcgcacg gatgatgtcc tggctgcgtt cgtccagacc atgcatcgcg tcggtcagac
    30181 ggtttgccgc ctgctcttcc cagttatcat cttcgatgcc gtcggcaaag ttagatgatt
    30241 tatcctgcag atagagcacc ggtgccatcg gctggctgtc ggaatcgtcg tcggaagaca
    30301 ggtcaaaggt catgtcctgt gccgccatac gtgattccat ctcacgtacg tctttgctgg
    30361 ttacgcccag ttcacgggcc accatttcga cttcatcctg gttaaaccag cccagacgct
    30421 gcttggtttt acgcaggttg aagaacagtt tgcgctgcgc tttggtggtc gcaactttga
    30481 cgatacgcca gttacgcaga acgtattcgt ggatctctgc tttgatccag tggacggcga
    30541 aggagaccag gcgcacaccc acttccgggt taaaacggcg cactgctttc atcaggccga
    30601 tgttaccttc ctgaatcaaa tccgcctgtg gcaggccata gcccgcataa ttacgagcaa
    30661 tatgaacaac aaaccgcagg tgagacagga tcagcgtttt agctgcttcc agatcgccat
    30721 ggtaatgcag cttttcagcc agcgcccgct cctcgtcagc cgacaacatc ggccacgcgt
    30781 tagctgcccg gatgtaggaa tccaggttgc caactggggc taaagctaaa ctttgcattt
    30841 tgtcagtcat tcaaatcctc tcaatcgata tcttctggcg cttcagtggt agcaacaact
    30901 gtgccaaagc ttaagagcaa cgaggttatc attcactgtt ttatcagacc gtgattttat
    30961 ccacaagttc aatgcaagct tgtgaataaa ttacgcacaa aatgtgacat agagatgaaa
    31021 taccgggaag agacaacggg gtctctttcc ctgctacgga acccattgca gggaaagagt
    31081 ataacacgct tttattattc aggcgtaaag tggcgtaaat gttgtaccgt ggcaagccac
    31141 gctgccaccc agccaatcat cgagcatacc agcagcaata gcaggcattc atcgaacgat
    31201 aaaccattga tatcaaactt cgttccgaaa acctgtgcca cttccgccac cgctgacgac
    31261 aaacgcagta ccagaatttc tgacaaaatt aatgacaaca atgcgccaga aaatcccagc
    31321 agcgcaccgc cgtacaggaa cgggcgaagg atgaaaccat ccgtcgcacc aatcagtttc
    31381 tgcacgttga tggagtcacg gcgagcaaag atgctcagac gtacgctgtt gccgatgacg
    31441 aggaacactg ccgccaccat caacacgccg atcatcgccg aaacgcgccc aaccagcccg
    31501 gtcaacgccg ccagacgggc aaaccagctg tcatccatcc gcacttcgtc aataccgtta
    31561 atctgcgtga tacgatcacg cagcgtattg agtgattccg tcccctgaaa atcgagtttc
    31621 gggatcacca ccgccactgc cggaagcggg ttttcttcca gcatatccag cgcaccacca
    31681 aaaccagacc agttacggaa ctcgcccagt gcgtcttcac gagaaagata gttcactttc
    31741 tccacgcctt gctcggcctg caactgtgcc accacgcccg cagcggcgtc atcgtccagc
    31801 gttttttgca agtaaacagt gatttgcggt gacggataat actgcgtcgc cgcctgatta
    31861 acgtttttgt acaccatata acagacgctg ggcagcgtca gggagatggc gataaccatc
    31921 accgttaaaa acgtggcgaa cggtttgctt ttcagatcct gcaatgcgcc gtggaaggca
    31981 tagcgcacct gttcgttgaa aacgttggtt ttgcgattta ccggttttgg cgaggatttc
    32041 gcgcgtttcg gtgcgttacg accgccgtcg cctgagccgc cgaccgattt acggaagcga
    32101 tccagacgcc caccaaactg ccgaatatga ttgattgcat cgcgcttatt catggcccac
    32161 gcctccatgc aagtgaccat cgctcagggt gagcatgcga taggaacgcc gtgaaatcag
    32221 attaatgtcg tgcgttgcca tcaatacggt taccccaacg cggttaaact cttcaaacag
    32281 acgtagaatc ccttccgaca acgcgtcgtc caggttaccg gtcggttcgt ccgccagcag
    32341 taccgcgggc ttattcacca ccgcgcgggc aatgccaaca cgctgttgtt caccgccgga
    32401 aagctgaata gggaagttct tcgctttgtc cagtagcccg actttatcca gtgccgccga
    32461 cacccggcga cgaatatcgt caccgctggc accagcgata atcagcggga tcgccacgtt
    32521 atcgtagaca gtacggtcca tcagtagatg gtgatcctgg aaaatcatgc caatctggcg
    32581 gcgcagaaac ggaacttcac ggtttttcag acgcgtgatg tcatggccgc taaaccagat
    32641 tttcccggcg ctgggccgct caatcccaca gatcagcttc aggagggtac ttttccctgc
    32701 gccggaatga ccggtcagaa acgccatctc acccggctgc atatggaacg taacgccctg
    32761 cagcgcctgt ctcccaccga gataagcctt gctgacatgt tcaaagcgaa tcattgttaa
    32821 tcctctcggg caaaaagtgc ctctataaag tcgtccgcct taaacggacg caaatcctca
    32881 atacgttcgc cgacaccaat gtagcggata gggataccaa actggtcagc caccgagaaa
    32941 attaccccgc ctttcgccgt gccgtccagt ttcgttagcg tgatgccggt taagccaaca
    33001 gcttcgtgga acagtttggc ctggcttacc gcgttctgcc cggtgctggc atcaatagtc
    33061 agcataactt catgcggcgc ttcaacgtcg agtttcttca tcacgcggac gattttcttc
    33121 aactcttcca tcagatgcga tttgttctgc agacgtccgg cggtatcggc aatcaggacg
    33181 tcgatattac gcgctttagc tgcctgaatg gcgtcgaaga taacagaggc ggaatccgca
    33241 ccggtatgct gggcaatcac cggaatattg ttgcgctgac cccagacctg aagctgttca
    33301 accgcagctg cacggaaagt atcgcccgcc gccagcatca ccgatttacc ctgctgctca
    33361 aactgacgcg ccagcttacc aatcgtcgtg gttttaccca caccgttgac gcccaccatc
    33421 aggatcacga acggcgtttt atcttcaaca ttcagcggct catcgacttt cgccagaatc
    33481 tcgcccatct cttctttcag caggccatag agcgcctcgg catcacgaag ctgcttgcgg
    33541 gatgcgcctt ccgtcagatt agtgataatt ttacgtgtgg tttccacgcc cacatcggcg
    33601 atcaacagct gttcttccag ctcttcaaac agatcatcgt cgattttttt accgcggaac
    33661 aggctgataa atccggaacc gagattttct ttggttttta acaggctgcg tttcaggcgc
    33721 gcgaagaagc cttctttggt cggtttttct tgctcctggg caatttctgc aaccggctgc
    33781 tcttcttctg ccggaggaac taccatcacc gcctcttctg ccgcttcggc agccagcgcc
    33841 tgtgcttcca gctcttcgtc ggtgatttct tctttagccg cttcttcttc tgccgcttcg
    33901 acaatctcta cggtttccgc ttcagcctgc cactcttctg acgaaacctc ttcggcgttg
    33961 acgtcttccg gcaacggcag ctcttcacgt tcgatagcca ctggctccgg cgtttcttct
    34021 acgaccggtt ccggctgtgc aacaacttcc gcttcaggct gcgctttttc actttcaaca
    34081 acctgttcag tgacttccac aacgtcggca gcaaaagttt ccgcctcggc ttcagtatgc
    34141 gcctgcggct gctcttcaac ggcttgttca gaagccttcg caggctcttg cgcctgaacg
    34201 atttcttcta caaccggttg ttcattctga acttctgtct ctttttccgg ggtctgctct
    34261 ttttgaccaa agcccagcca ggaaaaaaag ccacgtcttt tttctttcgc catttgcgac
    34321 tacactcctc gctgttgctt catggcacag cgttaacgct atgtacatag cagctaaaaa
    34381 aatgatgaaa tagtctatca cttaacttaa ttcacatcac cgcctgcaag tatgtgttat
    34441 ctggcggatt gagcaattta tcatgaaaaa accgaatcat tccggcagcg gccaaatccg
    34501 cattattggc gggcagtggc gaggccgtaa actcccggtt cctgatagcc caggtctgcg
    34561 ccccaccacc gaccgcgtac gcgaaacgtt gtttaactgg ctggctccgg tcattgttga
    34621 cgcccaatgt ctggattgct tcgccgggag cggcgcgctg gggctggaag cgttatcgcg
    34681 ctacgctgcg ggggcaacgt tgattgagat ggatcgcgtg gtttctcagc agttaattaa
    34741 gaatctggcg acactaaaag caggcaatgc acgcgtggtg aacagcaacg caatgtcatt
    34801 cctggcgcaa aaaggcacac cgcataatat cgtgtttgtc gatccaccgt tccgccgtgg
    34861 cttgttagaa gagacgataa atttactgga agataacggc tggctggctg acgaagccct
    34921 gatttatgtc gaaagcgaag tcgaaaacgg tctgcccact gttccggcaa actggtcatt
    34981 acatcgggaa aaagtggcgg gtcaggtggc ttatcggctg tatcaacacg aagcacaagg
    35041 agaaagtgat gctgattaat attggtcgtt tgttaatgct ctgcgtttgg ggatttttaa
    35101 tcctcaatct ggtgcatccc ttcccgcgcc cgctaaatat tttcgttaac gtggcgctga
    35161 tttttaccgt gctgatgcat ggtatgcagc tggcgctatt gaaatccact ttaccgaaag
    35221 atggcccgca gatgaccacc gccgaaaagg tgcggatttt ccttttcggc gtgtttgaac
    35281 tgctggtctg gcagaagaaa tttaaagtta aaaaataacc ttattgttcg ccaacaaagc
    35341 taacaaagcg cgttccttta tagctcagcg tacctttatc gcccactgtc agggcgtggt
    35401 actgctgggc gtcgaggcga aacgtctgct ccattcctcc gctttgcggt ttgaagcttg
    35461 cctcatagcg catactggtg cctgccggag tcacttcctg ctggcgcgaa cggcgatcgt
    35521 taatcggttt ttcccgcttg ttgctcacca ccaccagctt ttgctggagc ggagccatat
    35581 cattatcagc tttttcccgt cgctgttgca taaaacgaaa cgatgcggcg acgacaatta
    35641 agccaatgat aacaataaag aaaagaggtg gtttactcat ctttatccct catcggaaaa
    35701 tgcggaatta agcataccct gccagttatg gtgttgtcat ccgtccaccc tcgccactaa
    35761 actggaagca agaccgtagg cattctgctt acgaaaaaat aacgaattca aggaactaag
    35821 atgctttggt cgtttatcgc tgtctgtctt tccgcatggc tatctgtgga tgcatcgtat
    35881 cgtgggccaa cctggcaacg ctgggtgttt aaaccgttaa cccttcttct cctgctgtta
    35941 ctggcctggc aagcaccgat gttcgacgcc attagctatc tggtgctggc gggactgtgc
    36001 gcctcactgc tgggcgatgc gctaaccctg ttgccacgtc aacgtctgat gtacgccatc
    36061 ggcgcgtttt tcctctcgca cctgctgtac accatctatt tcgccagtca gatgacgctc
    36121 tctttcttct ggcctctgcc actggtgttg ctggttctgg gtgcgctgct actggcgatt
    36181 atctggacgc ggctggaaga gtaccgttgg cctatctgca cgtttatcgg catgacgctg
    36241 gtgatggtgt ggctggcagg tgaactgtgg ttcttccgtc cgaccgctcc ggcgctctct
    36301 gcgtttgttg gcgcttcgtt gctgtttatc agtaactttg tctggctggg gagccactat
    36361 cgccgacgct tccgtgcgga taacgcgatt gctgcggcct gctactttgc cggtcacttc
    36421 ctcatcgtcc gctcactgta tctctgataa aacttgactc tggagtcgac tccagagtgt
    36481 atccttcggt taatgagaaa aaacttaacc ggaggatgcc atgtcgacac ctgacaatca
    36541 cggcaagaaa gcccctcaat ttgctgcgtt caaaccgcta accacggtac agaacgccaa
    36601 cgactgttgc tgcgacggcg catgctccag ctcgccaacg ctctctgaaa acgtctccgg
    36661 cacccgctat agctggaaag tcagcggcat ggactgcgcc gcctgtgcgc gcaaggtaga
    36721 aaatgccgtg cgccagcttg caggcgtgaa tcaggtgcag gtgttgttcg ccaccgaaaa
    36781 actggtggtt gatgccgaca atgacatccg tgcccaggtt gaatctgcgg tgcaaaaagc
    36841 gggctattcc ctgcgcgatg agcaggccgc cgacgagcca caagcatcgc gcctgaaaga
    36901 gaatctgccg ctgattacgc ttatcgtgat gatggcaatc agctggggtc tggagcagtt
    36961 caatcatccg ttcgggcaac tggcgtttat cgcgaccacg ctagttgggc tgtacccgat
    37021 tgctcgtcag gcattacggc tgatcaaatc cggcagctac ttcgccattg aaaccttaat
    37081 gagcgtagcc gctattggtg cactgtttat tggcgcaacg gctgaagctg cgatggtgtt
    37141 gctgctgttt ttgattggtg aacgactgga aggctgggcc gccagccgcg cgcgtcaggg
    37201 cgttagcgcg ttaatggcgc tgaaaccaga aaccgccacg cgcctgcgta acggtgagcg
    37261 ggaagaggtg gcgattaaca gcctgcggcc tggcgatgtg attgaagtcg ccgcaggtgg
    37321 gcgtttgcct gccgacggta aactgctctc accgtttgcc agttttgatg aaagcgccct
    37381 aaccggagaa tccattccgg tggagcgcgc gacgggcgat aaagttcctg caggagccac
    37441 cagcgtagac cgtctggtaa cgctggaagt gctgtcagaa ccgggtgcca gcgccattga
    37501 ccggattctg aaactgatcg aagaagccga agagcgccgc gcccccattg agcggtttat
    37561 cgaccgtttc agccgtattt atacgccagc gattatggcc gtcgccctgc tggtaacgct
    37621 agtgccgccg ctgctgtttg ccgccagctg gcaggagtgg atttataaag gtctgacgct
    37681 gctgctgatt ggttgcccgt gtgcgttagt tatctcaacg cctgcggcga taacctccgg
    37741 gctggcagca gcagcgcgtc gtggggcgtt aattaaaggc ggtgcggcgc tggaacaact
    37801 aggtcgtgtt acccaagtgg cgtttgataa aaccggtacg ctgaccgtcg gtaaaccgcg
    37861 cgttaccgcg attcatccgg caacgggtat tagtgaatct gaactgctga cactggcggc
    37921 ggcggtcgag caaggcgcga cgcatccact ggcgcaagcc atcgtacgcg aagcacaggt
    37981 tgctgaactc gccattccca ccgccgaatc acagcgggca ctggtcgggt ctggcattga
    38041 agcgcaggtt aatggtgagc gcgtgttgat atgcgctgcc gggaaacatc ccgctgatgc
    38101 atttgctggt ttgattaatg aactggaaag cgccgggcaa acggttgtgc tggtagtacg
    38161 taatgatgac gtgctgggta tcattgcatt gcaggatacc ctgcgcgccg acgctacaac
    38221 tgccatcagt gaactgaacg cgctgggggt taaaggagtg atcctcaccg gcgataatcc
    38281 acgcgcagcg gcggcaattg ccggggagct ggggctggag tttaaagcgg gcctattgcc
    38341 ggaagataaa gtcaaagcgg tgaccaagct gaatcaacat gcgcccctgg cgatggtcgg
    38401 tgatggtatt aacgacgcgc cagcgatgaa agctgccgcc atcgggattg ctatgggtag
    38461 cggcacagac gtggcgctgg aaaccgccga cgcagcatta acccataacc acctgcgcgg
    38521 cctggtgcaa atgattgaac tggcacgcgc cactcacgcc aatatccgcc agaacatcac
    38581 cattgcgctg ggactgaaag ggatcttcct cgtcaccacg cttttaggga tgaccgggtt
    38641 gtggctggca gtgctggcag atacgggtgc gacggtgctg gtgacggcga atgcgttaag
    38701 gttgttgcgc aggagataag gcaaaccgat cgcaacattg agcgcgatcg gtcccctcgc
    38761 ccctctgggg agagggttag ggggtgagga aaaggcggca tcgaagccaa tcagccccta
    38821 tcaaccgcct ttacgaatca aataacgata aggcagtcca tccgtctctt tcgcaaccag
    38881 ttcgtgttcc ataaaggtac aaaacccagg aatatcgcgg gtagtggccg gatcgtcggc
    38941 gataatcagc aacgtttcgc caggctgcat attgcgcacg gttttgcgca ccatcatcac
    39001 cggttccggg cagcgcaggc caagcgcgtc gagtgtgtgg tcagggctgg aaaagagatc
    39061 ggtcattttc ttctcatcac ttaaaaaaac ggcgctagtt tacgccctgt gagtccgtaa
    39121 gcaaaccagg ttaacgattg cgtgaaaatt aaccattgca ttgtcaacgt aaagcagtat
    39181 catgcggcgg ctcgaaaaaa gggtaagcac gttattatgt taaggtaaca gacgtgtcgt
    39241 acgtattggg ttccctcacc ccaatggtta atcaaaaagg tacaatatga acgttttctc
    39301 gcaaactcaa cgctataagg cgttgttctg gttatcgtta tttcatcttc tggtgatcac
    39361 ctccagtaac tatctggttc agcttcccgt ctccattttt ggtttccata ccacctgggg
    39421 cgcgtttagc tttccgttta tttttcttgc taccgacctg accgtgcgta tttttggcgc
    39481 accgctggcc cgacgcatta tcttcgcggt aatgatccct gcgttattaa tctcctacgt
    39541 catctcgtcg ctattctata tgggttcctg gcagggattc ggcgcactcg cccacttcaa
    39601 cctgtttgtc gcccgtatcg ccaccgccag tttcatggcc tacgcgctgg ggcaaatcct
    39661 cgacgtgcac gtttttaacc gcctgcgtca gagtcgccgc tggtggctgg caccgacagc
    39721 gtccacactg ttcggtaacg tcagcgacac gctggccttt ttcttcattg ccttctggcg
    39781 tagcccggat gcctttatgg ctgaacactg gatggaaatc gcgctggtcg attactgttt
    39841 caaagtgtta atcagtatcg ttttcttcct gccaatgtat ggcgtattac tcaatatgct
    39901 gttgaaaaga ctggcagata aatccgaaat caacgctttg caggcgagtt aaaggttcgt
    39961 tatcagagtt gtgataagat ggatgaatga gccgttatgg ccgtttatcg aaaggaagaa
    40021 gtcaatgcgc aatctggtta aatatgtcgg aattggcctg ctggttatgg ggcttgcggc
    40081 ctgtgatgat aaagacacta acgctacggc gcagggttcg gttgcggaaa gtaacgctac
    40141 cgggaatccc gtcaacctgc ttgatggcaa gttaagtttc tcgctgccag cggatatgac
    40201 cgaccagagc ggtaagctgg gaacgcaggc caataacatg catgtctggt ccgacgccac
    40261 cgggcagaaa gcagtcatcg tcatcatggg cgatgatccg aaagaagatc tggcggtgtt
    40321 ggcgaagcgt ctggaagatc agcaacgtag ccgcgatccg cagctgcaag tggtaaccaa
    40381 taaagccatt gagctgaaag gtcacaaaat gcagcagtta gacagtatta tctccgcgaa
    40441 aggccagacg gcgtactctt ccgttattct gggtaacgtg ggtaatcaac tgctgaccat
    40501 gcaaattacg ctgccagctg acgatcagca aaaagcgcaa accaccgcag aaaacatcat
    40561 taatacgctg gttattcagt aagttttaag atgatgaggc ggcctcaggg acgtgttccg
    40621 gaggccgttt ttttaatcgc cacgtcagta ataacgcgat tgcgaccagt cccgccgccg
    40681 ccagataaat caccggtacg cccgcccagc tcatcaccag cccagccagt ggtccagtca
    40741 cgccaagcga taaatccata aatacggtgt aagttgccag cgccgccccc tgattttgct
    40801 gcggaaccgc ttttaccgcc actacaccca atgccgggaa caccagcgaa aacccggccc
    40861 ccgccagtaa gacgccgatt ttcgccatcc acggcatagt cgccacgcca accagtagca
    40921 ggccgattat ctcaacgcta aagcaaatca tcgctacgtt taagccaccg atacggttaa
    40981 tgccgttagg gaataacaaa cgcgtaccga caaacgcaca gctaaacagc gtcagcgcga
    41041 aagccgcacc gtcccaacct ttagcgtcat aaaacagcgt gataaaggtg gcgatgacgc
    41101 caaatccggc ggaagccagt gccagcgcca taccgtacag ccagacgcgc ccaagcaccg
    41161 cgcgaaacgg cagcggtttg cctttactgg cttttaccgt cggacgcggg atcgccaaca
    41221 aaatggccac cagcgccacg cccataatga ttaacgctaa cgcctgcaag ccgccccagt
    41281 gataaaacac gacgcctaac ggcgcaccca tcgccatcgc cccgtaagtg acaatgccgt
    41341 tccacgaaat cacccgcccg atatgcagcg agccaaccac tccaacgccc catagggtcg
    41401 atcccgttcc ggcaaaactt tgcccaatcc caaggatgac gcgccccagg caaagtaata
    41461 acaggctgat gacaggcaga ctggcggtta atcctgccgt cagatacccc agaccgctca
    41521 aaaagcagcc gcataaaccg aagacgacaa tctttttggg tcccagcaaa tcggcgtaac
    41581 gtccggcatg agggcggctc agcaaggtgg cgaaatattg caggctgata accaatcctg
    41641 cccagaaggc gctaaagccc atcacatcat ggacatagcc cggtaataca gcgagcggca
    41701 acccgatggt gaggtagctg gcgaagttaa acatgacaat ggagacaatg cgcaaattca
    41761 ggcgcaatcc gtttagcgcg ggttcggcta cgggttcggg catgaggatc accacatttt
    41821 tacaacagtg tttcattttt accatgtgct gacgtgaaaa tcagcagtaa gaatcagaat
    41881 attgctggcg tgactcccgc gctacactta acacaaaaag tcacaaggaa gccccaatgg
    41941 aaacccctca acccgataaa acgggcatgc acattctgct caagttggcc tcgctggtag
    42001 tgatcctcgc gggcattcac gcagcggcag atatcattgt gcagctgtta ctggcgctgt
    42061 tttttgccat cgtcctcaac ccgctcgtta cctggtttat tcgtcgggga gtacaacgcc
    42121 ccgttgccat tacgattgta gtggtggtga tgctgatcgc actaaccgcg ctggtcggcg
    42181 tactggcggc atcgtttaac gaatttatct ctatgctgcc gaagtttaat aaggagctga
    42241 cgcgcaaact ttttaaattg caggagatgt tgccttttct taatttgcat atgtcgccgg
    42301 agcgaatgct gcagcggatg gactcggaaa aagtggttac cttcaccaca gcgctaatga
    42361 ccgggctttc cggggcaatg gcgagcgtgc ttttgctggt gatgaccgta gtttttatgc
    42421 tgtttgaagt gcgccacgtc ccttacaaaa tgcgttttgc gctgaataac ccacagattc
    42481 acatcgcagg attacaccgc gcacttaaag gcgtttcgca ttatctggcg ttaaagacgc
    42541 tactcagttt atggacaggt gtcatcgtct ggctggggct ggcgctaatg ggcgtacagt
    42601 ttgcgctgat gtgggcagta ctggcgtttt tgctcaacta cgtgcccaat atcggcgcgg
    42661 taatttccgc cgtaccgcca atgattcagg tgctgctgtt taatggtgtt tacgaatgta
    42721 ttctggtcgg cgcattgttt ttagtggtcc atatggtcat cggcaatatt ttagaaccac
    42781 ggatgatggg ccatcgcctg gggatgtcca ccatggtggt atttctttca ttgttaatct
    42841 ggggatggct gctcggcccg gtagggatgc tactttcggt gccattaacc agcgtgtgta
    42901 aaatctggat ggaaacgacg aaaggtggca gcaaactggc gattttactg gggccgggca
    42961 gaccgaaaag tcggttaccg ggatgaggcg acaagtgata cgatacgcac tttcattttc
    43021 cattaaacgt tggccctgat atgtatcgga tagttctggg gaaagtttcg accttaagcg
    43081 cagctccact gccaccgggt ttacgcgagc aagcaccgca aggtccacga cgcgaacgct
    43141 ggctggcggg gcgtacattg ctttcgcaca cgctttcccc gctaccggag atcatctatg
    43201 gcgaacaagg caaacctgca ttcgcgccgg aaacgccgct atggttcaac ttaagccata
    43261 gcggtgacga tatcgctctg ctgttgagcg atgaaggcga agtcggctgc gatatcgaag
    43321 tgattcgccc gcgcgtcaac tggcgctggc tggcgaacgc cgtgttcagc ctcggggaac
    43381 acgctgaaat ggacgccgtg catcctgatc agcaactgga aatgttctgg cgcatctgga
    43441 cgcgcaaaga agccatcgtt aaacagcgtg gcggcagcgc ctggcaaatc gtcagcgtag
    43501 atagcaccta tcactcctcg ctgtcagtca gccattgtca gctagaaaat ttaagccttg
    43561 cgatctgcac ccccaccccc tttacgctca ccgccgacag tgtgcaatgg atcgattcag
    43621 ttaactgatc cgcccacccg actgcccatc tattgatcca gaacaggtaa tcagtatgac
    43681 gaatacttaa aatcgtcata cttatttccg ccatctattt taatccattg gggttaccat
    43741 gctctccaca ctccgccgca ctctatttgc gctgctggct tgtgcgtctt ttatcgtcca
    43801 tgccgctgca ccagatgaaa tcaccaccgc ctggccggtg aatgtcgggc cactaaaccc
    43861 gcacctttac acgcctaacc agatgttcgc ccagagcatg gtttatgaac cattggtgaa
    43921 atatcaggca gacggttcgg tgatcccgtg gctggcaaaa agctggactc attcagaaga
    43981 tggtaaaacc tggaccttca ccctgcgtga tgacgtgaaa ttctccaacg gtgaaccgtt
    44041 cgatgccgag gcggcggcag aaaacttccg cgcagtgctc gataaccgtc aacgtcacgc
    44101 ctggctggag ctggcaaacc agattgttga tgttaaagca ctcaataaaa cagagctgca
    44161 aattaccctg aaaagcgcct actatccttt cctgcaagaa ctggccctgc cccgtccttt
    44221 ccgttttatc gctccctcgc agtttaaaaa ccatgaaacc atgaacggaa ttaaagcgcc
    44281 gattggcacc ggaccgtgga ttttgcagga atcgaaacta aatcagtacg atgtcttcgt
    44341 ccgtaacgaa aactactggg gcgaaaagcc agcgattaaa aagatcacct ttaacgtcat
    44401 cccggacccg actacccgcg cggtggcgtt tgaaactggc gatatcgacc tgctgtacgg
    44461 aaacgaaggg ttattaccgc tcgatacctt cgcccgcttt agccagaatc cggcttacca
    44521 cacccaactg tcacagccga tcgaaaccgt gatgctggcg ctcaataccg ccaaagcccc
    44581 caccaacgag ctggcagtac gtgaagctct taattacgcg gtaaacaaaa aatcgctgat
    44641 tgataacgcg ttatatggca cccagcaggt cgccgacacc ctgtttgccc cttctgtgcc
    44701 ttacgctaat ctcggcctga aaccgcgcca gtacgatccg cagaaagcga aagagttgct
    44761 ggaaaaagcc ggttggacgc tgcctgcggg caaagacatc cgcgagaaaa atggtcagcc
    44821 gctgcgcatt gaactgtcgt tcatcggcac cgatgcgtta agcaaatcga tggcggaaat
    44881 cattcaggct gatatgcgcc agattggcgc agatgtctcg ctgattggcg aagaagagag
    44941 cagtatctat gctcgtcagc gcgacggtcg ttttggcatg attttccacc gcacctgggg
    45001 cgcgccatac gatccacacg ccttcctgag ttcaatgcgc gtaccgtcgc acgctgactt
    45061 ccaggcacag caaggattag ccgacaagcc gctgattgat aaagagatcg gcgaagtgct
    45121 ggcgacccat gacgaaacgc aacgtcaggc gctgtatcgc gacattctga cccgtctgca
    45181 tgacgaggcg gtttatctgc ctatcagcta catctcgatg atggtggtat caaaaccgga
    45241 gctgggtaat atcccctacg cgccgatcgc caccgaaatt ccgttcgaac agattaaacc
    45301 ggtgaaacct taatgttgcg ttacgtatta cgccgctttc tgctgctgat cccgatggtg
    45361 ctcgccgcct cggtgatcat ttttctgatg ctgcgcctcg gtaccggcga cccggcgctc
    45421 gattatctgc gtttatctaa cctgccgccg acgccggaga tgctggcctc tacccgcacc
    45481 atgctcgggc tggatcagcc gctgtacgtc cagtacggta cctggttgtg gaaggcgctg
    45541 catcttgact ttggtatctc attcgccagc caacgcccgg tactggacga tatgctgaac
    45601 ttcctgcccg ccacgctgga acttgcaggt gcggcgctgg tattaattct gctcacttcc
    45661 gtaccgctcg gtatctgggc ggcgcgccac cgcgatcgtt tgccggattt cgccgtgcgc
    45721 tttatcgcgt ttcttggcgt gtcgatgccc aacttctggc tggcattttt gctagtgatg
    45781 gcgttttcgg tgtatctgca atggctaccc gcgatgggct acggcagctg gcagcacatc
    45841 attttacctg cggtttccat cgcctttatg tcgctggcga ttaacgcgcg actgctgcgc
    45901 gccagtatgc tggacgtcgc cggtcagcgt cacgtcacct gggcgcggct acgcggcctg
    45961 aatgacaaac agaccgaacg ccgccatatt ctgcgcaatg cctcgctgcc gatgatcacc
    46021 gccgtgggga tgcacattgg cgaattgatt ggcgggacga tgattatcga aaacatcttt
    46081 gcctggcctg gcgtcgggcg ctatgcggtg tcggcgattt ttaaccgtga ctatccggtg
    46141 attcagtgct ttacgctgat gatggtggtg gtttttgtgg tctgtaattt gattgtcgat
    46201 ttgctcaacg ccgcgctgga cccgcgcatt cgtcgtcatg aaggagcgca cgcgtgaact
    46261 ttttcctctc ttcccgctgg tcggtacgcc tggcgctgat cattatcgcc ctgctggcgc
    46321 tgattgcgct caccagccag tggtggcagc cgtacgatcc acaggcaatt gatttgccgt
    46381 cgtgcctgct ttcgccggat gcgcagcact ggctgggcac cgatcactta ggtcgcgata
    46441 ttttctcgcg gctgatggca gcgacccgcg tgtcgctcgg ttcggtaatg gcctgcctgc
    46501 tgctggtgct gacattaggg ctggttattg gcggcagcgc cgggttgatt ggcgggcacg
    46561 ttgatcaggc caccatgcgc gtcgccgata tgtttatgac cttcccgacc tcgattctgt
    46621 cgttctttat ggttggcgtg ctcggcaccg ggctgaccaa cgtaattatc gccatcgccc
    46681 tgtcgcactg ggcgtggtat gcacgcatgg tgcgcagctt ggtgatttca ctacgccaac
    46741 gcgagtttgt gctggcgtca cggctttccg gtgcgggcca tgtgcgggtg tttgtcgatc
    46801 atctggcagg cgcggtgatc ccttcgctgc tggtgctggc aacgctggat atcggccata
    46861 tgatgctgca cgtcgcgggg atgtctttcc ttggcctcgg tgtgaccgcg ccgaccgccg
    46921 aatggggcgt gatgattaac gacgcgcgcc agtatatctg gacccagccg ctgcaaatgt
    46981 tctggccggg gctggcgctg tttatcagcg tgatggcctt taacctggtg ggtgacgcac
    47041 tgcgcgatca tctggatcct catctggtga cggagcacgc acactaatgc cgcaacagat
    47101 tgaactacgt aatatcacgc tacaggccgc gcagccgctg gtacacggtg tatcgttaac
    47161 cctgcaacgc gggcgcgtgc tggcgttagt cggcggtagc ggcagcggga aatcattaac
    47221 ctgcgccgcg acgctgggca ttttgcccgc tggcgttcgc cagacggcgg gggaaatttt
    47281 agccgatggc aaaccggttt cgccttacgc cctgcgcggc atcaaaattg ccaccatcat
    47341 gcagaacccg cgcagcgcct ttaatccact gcacaccatg cacacccacg cgcgggaaac
    47401 ctgcctggcg ttagggaaac ccgccgatga cgctacgctt accgctgcca tagaagcggt
    47461 ggggctggaa aacgccgcgc gcgtgctgaa gctgtacccg ttcgagatga gcggcggcat
    47521 gttgcagcgc atgatgattg cgatggcggt gctgtgtgaa tcaccgttta tcatcgccga
    47581 tgaaccgacc accgacctcg acgtggtagc acaggcgcgc atcctcgatc tgctggaaag
    47641 cattatgcaa aaacaagcgc cgggaatgct gctggtcacc catgatatgg gcgttgtggc
    47701 gcgtctggcg gatgacgtgg cggtgatgtc acaaggtaaa attgtcgaac agggcgatgt
    47761 agaaacgctg tttaacgccc ccaaacatac ggtgacgcgc agcctggttt ccgctcatct
    47821 cgccctctac ggtatggagc tggcatcatg accttactta acgtctgcgg cctttcccat
    47881 cactatgccc acggtggatt tagcggaaaa catcaacatc aggcggtgct gaataacgtt
    47941 tccctgaccc tgaaaagcgg cgaaaccgtc gccctgctgg gacgcagcgg ctgcggaaaa
    48001 agcaccctcg cgcggctgct ggtaggctta gagtctccct cacaagggaa tattagctgg
    48061 cacggcgaat cgctggcgaa acttaatcgc gcccaacgta aagccttccg ccgtgatatt
    48121 cagatggtgt ttcaggactc catcagtgcc gtgaatccgc gcaaaaccgt gcgcgagatc
    48181 ctgcgtgaac cgatgcgcca cctgctatca ctgaaaaaat ccgaacaact ggcgcgcgcc
    48241 agcgaaatgc tgaaggcggt cgatctcgat gacagcgttc tcgacaaacg cccaccgcag
    48301 ttaagcggcg gccagctcca gctcgtctgc ctggcacgcg cgctggcggt cgaaccgaaa
    48361 ttgctgattc tggatgaagc cgtttctaac cttgatctcg tgttacaggc gggcgtcatt
    48421 cgcctgctga aaaagctaca acaacagttt ggcactgcct gcctgttcat cactcatgac
    48481 ttacgcctgg tggaacgctt ttgccagcga gtgatggtga tggacaacgg acaaatcgcc
    48541 gaaacccagg cggtgggaga taaattaacc ttttcctctg acgccggacg tgtgctacaa
    48601 aacgcggtat tacccgcatt ccccgtgcgc cgtcgcacct cagaaaaggt ttaacgccaa
    48661 tgcaacgagt caccatcacg cttgatgacg atttactgga gacgctggac agcctgagcc
    48721 agcgtcgtgg ttataacaac cgttccgaag ctatccgcga tattctgcgt agtgccctgg
    48781 cgcaggaagc tacccagcag cacggcacac aaggtttcgc ggtgctgtcg tatgtgtatg
    48841 aacacgaaaa acgcgactta gccagccgta ttgtttccac ccagcatcat caccacgacc
    48901 tctccgtcgc cacgctacat gtgcacatca accacgacga ctgtctggaa atcgccgtgt
    48961 tgaaaggtga catgggtgac gtgcagcatt ttgccgatga cgttatcgcc cagcgcggcg
    49021 tgcggcacgg gcatttgcag tgcctgccga aggaagattg agttatttct aaaacaagga
    49081 aagaaaagat attaactgaa cttaatttat aaaattaagt tcagttgcat agatagatcc
    49141 tgtaaaggca gatattctgc gtaatgtacg tcgctaaaat ttataaatga ttcaaacgct
    49201 cattcagtgt ttcaataata taggtattaa cagagacttg ttgctgcgca gccgcattat
    49261 taaggcgttc actcagcgat tctggatagc gtaacgtaaa ggtcttaatc ttttctgtac
    49321 gcgcatacgg ctcaataccc gcagctttac aatcctcaag atactctcgc agggaaagct
    49381 ctccttcttt ttgcagcccc tgaatactgt cagagacaaa atcacaataa cctgacaagc
    49441 ccaggaattt gccacgaaat gcattcagtt ctggcacgta ggtaataaca gctggttgcc
    49501 cggcaatctc catagagttt ggcgtcttca gtttgatcat ggttttaccc ctatacttag
    49561 taaccagtca cacacgcttt ctacagcgcc tttatcggta tctggcgacg gatgaggccg
    49621 atggaaacac gcaacgctca tatttagtat gaacttgcaa cgcgaacctc ttccttcctt
    49681 aatttctccg cctaatgctt taaccagtga ctctatatca gaccacttga tcccttgagg
    49741 aacaggagtt ttaaatattt gatccaatgt atttttttgt ttcttccgta aagcctgtac
    49801 cttggttctc atggcatgtc cttatgactt cactttatga catcacagta tgaagtcaag
    49861 tttcccttcc ttacgccatt gtcccaatcg tcttcctgaa tcgcagcagt gcgatggtga
    49921 aaaacgcgcc gccaattgcc atcagcgtca ggaactgcgg ccagacgatt tcgaatccgg
    49981 caccccggta gaggatggcc tgcgcgaggc taacaaagtg tgtcgtcggc atggtcagca
    50041 taatgtcctg caccatctgc ggcatacttt cgcgcggcgt ggatccaccg gaaagcattt
    50101 gcagcggcag cagcaccaga atcaccagca gccccagttg cggcattgaa cgcgctatcg
    50161 tccccataaa aatgccgatt gacgtggtgg caaacagact gagcgccacg cccagcataa
    50221 acagcgggat cgagccttca atcggtacgc ccagtacacc tttcaccatc agcaccagcg
    50281 ataatcccga taccaccagc accaccagcc ccatcgacca gatcttcgcc atcatgatct
    50341 caaacggcgt tatcggcatc accagtaaat gttccaccgt gccgtgttca cgctcgcgga
    50401 tcagcgccga tccggtcaat acaatcgcca gcatggtaat gttgttgatg atcgccatca
    50461 ccccgccaaa ccacgcggga tcaaggttcg ggttaaagcg catccgggtt tccagcgata
    50521 ccaacggttc gctgttatca cggtagcgcg cgacaaagct gttcacttca ccgttgataa
    50581 tattctggat atacccattg ccggtaaatg cctggctcat gcgcgtggca tcgacgttca
    50641 cctgaatatc cggctggcgt ccggcgagga catcacgctg aaaattaggc ggaatattta
    50701 tcgcgaaggt atagcgtccg gcgtccagtc cggcatccat ctcatcggcg gtgatcatct
    50761 ccggtggcaa aaaccacgga cgatagaagc tgttaacgat ccggttcgat aactgcgatt
    50821 gatccatatc ggcaatggcg atcggcgcga ggttcaacga tcctggcgta acggtcgctg
    50881 acgaatacac cgacaccgta aacgagaaga caatcagcgt cagcatcgct ttatcaccga
    50941 gcagactgcg caactctttg atacccagat taaaaatatt gcgtaaatgg cgcatcatcc
    51001 ctcctgtttt ttcagcagca ggatgcttaa gcccatcacc agcgggatgg ctatcagcaa
    51061 cgggataaaa agttgccaca aatcagtcag atccagcgct ttcgagaacg ttccgcgggc
    51121 gatagtcaga aaatgactgg tcgggtaaac ctcgccgatc caacgtccag gcccttccag
    51181 cgaagctacc ggatcgatca tcccggaaaa ctgtgtcgcc gggatcaatg tgataatcgc
    51241 cgttccgaaa atggcggcaa tctggctttt cataaaggtg gagatcagca gccccattcc
    51301 agtggcaatg atgatataca gcagcgccgc caaggtgagc gtcaggaaac tgcctttatg
    51361 cggtacgcca aacacaaaca ccgacaggcc gcagagcagg aaaaagttca gcatccccag
    51421 cgcgatgtac ggcagctgtt tgccaagcaa aaattcgcta cgggtggtgg gggtcacgta
    51481 aaggttgata atcgacccta gctctttttc ccgcaccacg ctaagggcgc ttagcattga
    51541 cgggatcatc atcagcagaa gcgggatcac cgccggaaca atcgctggca ggctttttac
    51601 gtccgggtta tagcgataac gcgtctcaat gttcatcagc ccgctttggc tggcgggtgt
    51661 cgattgtcgg ctcgccacat cctgaagcca actctggtgc attgcctgca cgtaaccttt
    51721 taccgtttcg gcacgactgg gcatcgctcc gtcgatccag acgccgagtt ccacaggcgt
    51781 accacgcgcg atatcgcgcc cgaaattggg cgggatctca atggccaccg tgatatcgcc
    51841 cgcacgcatc cgacgatcaa gctcgtcata actggtgagc ggcggctgtt cgataaagta
    51901 acgggaaccg gagaggttga gtgtccacgc ctggctactg acggtctggt cgcggtcgag
    51961 caccgcaaag cgcaggtttt ccacatccat actgatgccg taacccatta tcagcatcag
    52021 gatcaccgtt cccatcagcg ccagcgtcga acgtactgga tcgcgtcgca gttccagcgc
    52081 ttcgcggcgg ctgtagctaa acagacggcg caggctaaat ccctgacgcg gcgcgtgggt
    52141 ggtgtcgtgt accacgggcg gcgcttcggc ttcgttgctc tgccctgccg cttcctgcaa
    52201 ataggcgata aatgcctctt ccagactggc ggctccgcgt ttctcaacca gttcctgcgg
    52261 tgtaccgctg gcaagcactt ttccggcgtg catcagtgag atgcggtcgc aacgttccgc
    52321 ttcgttcata aagtgggtgg agatgaagat agtcactttg tcctggcgcg agagatcgac
    52381 catcaactgc cagaacatat ccctcgccac cggatcgaca ccagaagtag gctcatcgag
    52441 gattaacatc tccgggcgat gaatcaccgc caccgccagc gaaagccgct ggcgaatgcc
    52501 gagcggcaat gactccggca gaacatcttc aacgtcgttg agcttaaaac gctcgctcat
    52561 ttcagccact cgcgcgggaa tttccgcttc cgggatgtga aacaaacggg catgtaactc
    52621 caggttttgc cgcacggtga gttcgttata gagcgaaaac gcctgcgaca tatagcccac
    52681 acgacggcgg gtatcgatat cttttggatc aaccggttgc ccaaatagcc acgcctcacc
    52741 ttcgctggcg ggcagcagtc cggtaagcat tttcatggtg gtggatttac cgcagccgtt
    52801 cgaaccaagg aatccaaaaa tctccccgcg tggaatgcgg aaattgacgt gatcaacggc
    52861 aacgaaggaa ccaaaacgca tggtcagatc gcgcgcttcg atggcaatct ctgcgttttc
    52921 aggttgatac ggtgggatca ctaccgcctg atgcgcctgg cgttgcgctt gcggtaacag
    52981 attaataaat gcttcttcca gcgtggcgct ttgcgtttgc tgccgcagct cttcggcgct
    53041 gccggttgcc agcacttctc cggcattcat cgctaccagc cagtcgaagc gttcggcctc
    53101 ttccatatag gcggtggcga ccagcacgct catattgctc tgccgctggc gaatactgtc
    53161 gatcagatcc cagaactggg cgcgggagag cgggtcaacc cccgttgttg gctcatcaag
    53221 gatcaacagt tccgggtcgt ggattaacgc gcagcacagc ccaagttttt gcttcatccc
    53281 gccggagagt ttacctgccg gacgatcgcg aaacggtgct aacccggtgc tggtcagcag
    53341 ctcattaatt cgcgcttccc gctccgcttt atcgtgaccg aacaggcgag cgaaaaaatc
    53401 gacattttca tacaccgaca aggtgtggta gaggtttttg cccagcccct gcggcatcca
    53461 ggcgatgcgc gggcagacgt ctcggcgatg cttcgggtcg tgcatatcgc cacccagcac
    53521 caccacattg ccctgctcaa tgacgcgggc accggaaatc agtgacaata aactcgactt
    53581 cccgacgcca tccggaccaa tcagcccgac catgcaacgg gcctgaatat cgagagtgat
    53641 attgttcagc gcaacggttt ttccataatg ctggctcacg cccgccagtt gcgcgacagg
    53701 cgggacggga accagttcca gatgcgtcat tgcggcaacc tcaccacgag gtcgtcaggc
    53761 cacggaagtt cttcattcac ccgcacccac gctacgcccg gtaaaccggt tttgacatat
    53821 tccagatgct gctggagtaa ttccggtggg atacgcgctt tgacgcggaa catcagtttc
    53881 agccgttcat cgctggtttc gacggttttt ggcgtgaact gggcgacact ggcgacaaaa
    53941 ctgatggttg caggaatacg cagatctggc gcggcatcga ggatcagccg ggcttcaccg
    54001 cccagtttca gcgtgcccgc ctgttcggtt ggcaggaaga aggtcatata gacgtcgctg
    54061 agatcgacca tattcagcac ccgaccgcct gccgccagca cttcccctgg ctcggcaacc
    54121 cgatactgca cgcgtccgtc acgcggggct ttcagttcac tgtcatcgat atctgcggca
    54181 atgcgccgtt cagtggcttg cgctgcttcg acccgagttt gcgcctgaat gatattggtg
    54241 cgtgctgctt ctatagccgc tttagaagcc gaaacctggg ctttcgctga ttccagcgca
    54301 gctcgggcgc tctcagcggc ggcgcggtcg tcatccagct gttgcgcaga aatagcccct
    54361 cgttgggcga gcgagcggga acgcgtatga cgtttggcaa cagagtccag ttcggcctgg
    54421 cgttgattga ccagcgactg tgcggcacga gtttcacttt gtcgttgctc cagcaaagcc
    54481 tgcgcggcgg caacggcgct ttgtgcctct ttgatttgcg cgatggcttc cagtcgctgt
    54541 tcctgcaaca cgcgagtatc catcttcgcc agcacttcgc cttcgcgaac aaactggcct
    54601 tctttcacca gaatggtgtc gatacgcccg gcaattttgc tggcgatatc cacttccgtc
    54661 gcttcaatgc gcccattgct gacagcaaag ccttccggca cacccgccgg gcgcaacagc
    54721 caccacgcca cgacggcagc caccgccagt gccccgacaa cccaccacgc cagatggcgc
    54781 ttactcttat ccataatcga cccgccataa tccctgtaag caaacgacgc gtgttacaac
    54841 tgccgccgaa cgacgttcag cggattcttc catccgggat aagaaggaaa cgacaaatac
    54901 gaccagcgcg cagagcattg agcgccaacg gggaaggctt agtgaaaacg ggtagtaatg
    54961 ttgtcatcat cagcattcct ggccgtaaat gaaacacgca tactcataag tgtgatctat
    55021 aaaactggct gacaaaacgg tatccatcct gttggatcgc atttcacgcc tgttcagttg
    55081 caggaacaga gcattccttt ttacgtttta tcaacagtct caaccagata gtaacattaa
    55141 tattcatgtt gtctatggtt cagagctgta acattgtgcg ccttctaatc aaaggcataa
    55201 gtccatttct gtttttttca tgctggtaag acttccaggg agtggttatc tctggccctg
    55261 caccagggtt aatttggcaa ttatgcatgc tgaacattct tccagcatgt ttactgtcac
    55321 gggcgttaag acctaaaact gtccccgttt cacctatgcg ttctctccca ccatttccgt
    55381 tatgtgcaac aacttgattt atacctaaca tttgtgaaca tgctaatagt gcttcaagtt
    55441 taggtctgta atctgtcgaa acagaaaaat aacgattaaa ggatcgacaa gagatagccc
    55501 ggctagtttt aaactttttg ccattaatta tagcatgatg ctttttatta ataatttccg
    55561 ctaattcgac cggatcgaga gcattggtca tttcatcaat agagacagtt atcggtccta
    55621 taatgtaacg ttttcgctga tcatcaaaag ttatcccatg atgatttgcc attattttgt
    55681 atttggggtc atagaaacat acaggataat cttttatccc gtcgtaggta tcctcatcaa
    55741 gtggtttcat gcgcgtgaag ttttgcatat agttaccatt gcaattcgtt tcatgatttc
    55801 cagctaatac atgaacattt tcattgatac tcattatgcg ttttaacaaa tcgatgataa
    55861 attgatcgcc acagatatta ctaaatcgat cgccggtttg gtcgccaaga aacgtacatg
    55921 gcgtgactgc gtctttatta ataaccaaat aaggctgaag actttctaaa acattatctc
    55981 ttctgttcag tatactgatt attgtattaa cattagtgtc aagttgctca ttaaagttct
    56041 cccttagcaa tacgttgaga acttcatctt ctatattaag caactggcta aggaaatttt
    56101 ctccttcagc catgattccc aaatgtcctg ttgcaaaaag cgcgtacaaa actgcaccga
    56161 ctgagccatc ggtatcgcca aaataggtcg gcctggtact tgtgggatca acaggtgaac
    56221 aattggagaa ataggttctg tcagcgaact ggggttttat aattgtttcg aatttaatat
    56281 caaatacatt gtattgcgat atttcccagt tccagatggg tttgtacgac tcagtaactg
    56341 caacatcatt agcattatca tttactgtca tattattaag taaataaaca atgctgcacc
    56401 aattaatatc ggtattatca agagggcaac cgtaattttt actattaatt gtcacaataa
    56461 actgataaca gttattccta cgtagaatat gagcgcgcat gcccgatagc atactatatt
    56521 tttctgaatg attcgtttgt atggtaaatg aaattttgtc atcttgaata gattgttgga
    56581 aacgttctat ttgccaatca tcaatttttt ccacaacttc accgagcatt gtcctggcaa
    56641 tacttgatat atcatcacct ataccatttg caatataccc aaacagttct ttagtgaagt
    56701 tggtaacatg ctcttgcacc atatcatttg ttgcgattgt cgttgtactg ccattagtgc
    56761 ctgccacagt tccaattttc atcttttacc tcatattatt caccgcgagg aatacatagt
    56821 taacatttta tcttatgaca tacacctcat attcgatggg gaaataatta tattttgcac
    56881 aaatcattca gccttaaagc ataatatgaa atttcttatc tcatagcaga gaaagtccct
    56941 aaatattgta gaggcgtcat cagtacatac ctctacaatc gattaagaaa acctatgaaa
    57001 tactggcaaa attaaaaaga gataccacag cactcatgaa gttttattga ggttgaaaac
    57061 aactaagccc tgggacacct tttttatcac cacgttcgtt aggtgctatc ttccaagaat
    57121 ttagccccat ggtataatta tcaatttttt gatgctggtc tacccctaat ccataatggc
    57181 caaatttctg tttagttttt ttgagcgtct gtccacattt gaagatatct tcaggttttg
    57241 gtctgaaagc agtagtgtta ttaaaataat tttcatatac aggtgtgggt gctatagcat
    57301 tgcttctaat tagatggaga agatgatcat ccatgtgttg tttatgtttt ttattacata
    57361 tatttgctaa ttcctcagga tcaattgggt ctttcatctg attaaatgga acctgcaaag
    57421 caccgagaca ataacattga ttttcttcat ttcttattat tccgtgatga ctggttaaaa
    57481 ctttagtttc tgagttataa ttacaaacat cgagcgtttt aattaagtta tacgtatcac
    57541 cggcagagag tttataattt tctagtcgtg cagtatagtt accattaaaa ttgatttcat
    57601 gatttccagc cagaacgatg acatttttat taatacggct atctttatta ccctccatat
    57661 ttcgcataga atttagcaaa gtaaggatat atttatcacc aaaaatagta ctaaatctat
    57721 cgcctgtatg atccccaagg aagatacaag gggttatcgc atcgtccttt aaaaaaacat
    57781 gttgatgcaa ctcattcaat atcatgtctc tcgtatttaa taaattgtag agcaatttat
    57841 tatttttatg tatgtatgca gatgaagcca tgtcttcgtg ttttaataat tcacataata
    57901 aactccaacc cttttccctg atgcctatat gccctgaaac gagcaatgaa taaagtacag
    57961 cgcctactct tccatctgta tcgccaaaat aagccgtgtt ggcattttgt gggttaatca
    58021 caccacattg tttataaaag tctattttag aaaacacaga tttaacatag ctatcaaact
    58081 tatccttagg aaacgtctct ttagagacat cccaacgcca gtcagcttta aaattaccgc
    58141 taagaagagt aaagaaattt tcaggtatga tagaaagccc attaacttta taaattatgt
    58201 ctgatttatc tccggagaaa ttaataaaat aataaccatc cttacactca atcttaactg
    58261 taatgggaga cgataatttt atcacatggt tattgtctgg tataattgta aagataaacg
    58321 tgttagtagt attatcggca aaactattat attcttgaat atctatagat ggctcactat
    58381 tgtaaaacca tattttctcc atctgaagac tatgttcatt taccctccag tgaccatgtt
    58441 gcaggaaata gatgatatgc atgccaaaat cgttatctat ttgcgaatca ttatccagct
    58501 tatcaatttc aactatcttt gaacgatgtg ttttatcaac cttgctatgg tgttgatgat
    58561 tattaacttt atctgaatgc ttatctttat ggtttgaagg gatgtactta aatatattgt
    58621 caattgcttt cataaataac ccgcgatatt tttgacctgt tagtaaaaaa taaatgatac
    58681 atgtctgaaa gacatattta cgacgatata aaacaataat acttatatgt tcgctgattt
    58741 taagcaccga gactatccgg tcttttacta aataatggaa aacaatccat ccctggaaga
    58801 caattgtaaa aaaaaacaac agaataagaa ttaatcttag gataaatatt tatttatcat
    58861 ggccttttga ccgctaaaaa atggcactcg gcacagtaaa ccccgctata gttatgctat
    58921 gcatttgtct ttaatgatat ctcgtcgtta tattaacagg ctgaaattat cattaatgca
    58981 catttcaata ttagcaggga tacctgagga gtttaaagtg aacatatata tcgggtagct
    59041 tttcaaatta atccctttga ttataggctt aatttgcatc gcgttaggtg gctttgtgct
    59101 ggaaagttca gggcaacgtc acgggtcatg tgctgatttc tctggcggcc atatgcctgg
    59161 cattattcac taccgcattt attatcattt cgcaactcac gcgcggcgtt aatacgtttt
    59221 acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg atatggggtt
    59281 gggcactgtt agcaggtaat gatgtgatgg cagacgagtt tgttgccggc catgttattt
    59341 ttggcgttgg tatgattgcc gcatgtgtat cgacggtggc agcgtcgtcc ggtcactttc
    59401 tgctcattcc caaaaatgca gcggggagca agagcgacgg aacaccggta caggcttatt
    59461 cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca
    59521 tttggtctat tacgctgtta catagtgccg acataactcc gcattatgtc gccttgggtt
    59581 aaccgcaatc tgtgcctgtc taattggcct agtggccaca atcgtccatc aaacacgtaa
    59641 tacgttctca tccaaagagc actggctgtg gtgttattgg gttatttttc tcggctcagt
    59701 cacggtgctg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    59761 cggcattatt ctcatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    59821 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgataccggt
    59881 atttacctgt ctgttttgct tgtttttgtc atcattcctc gcggaaatcg cacaaactga
    59941 gctggactac tttattccat ctcgggttct ggttggatta ggcgcagtat gttttacatt
    60001 gttctctatt gtttcaatat tagaggccgg ttctgcgaaa aaaaattaaa atttacataa
    60061 tcccagcgtt attcatatct taacgctggt tttttacgaa cttagtgaaa taatgtagaa
    60121 tataaaatta taattttata ttggagcaca tgtgttaatg atcaaccccg ggatactaaa
    60181 aatatagaca ttattaaact tgcttagcct cgcattgcct ttacctgtta aagatgcatc
    60241 gagaaaattg agtctgaagt tctcatcctt tatctgattt tccagtcccg aaactttggc
    60301 cttagcatta cctgagatcc gtccgttact atctattttg taggtaaaaa aatacgaatg
    60361 ggaaaatttc ctcggtgtat cttcatcagt taagccaaca atcgtaaaac taccgctacc
    60421 atcttttttt aaaatgactg aaaggttagc agagtagtat aaagaatgat ctgttttagc
    60481 atttcttgtt aacgtcgacg tacattcaag agacgttgtg ctaccatcac gagccttcca
    60541 gagagcaaaa aaacacacct gtagcaatgt gaaaacaagt attaatataa aatacatttt
    60601 cttattagtc atataatagc acctctgaac aaattgcata agtttcgtca ttagaggatt
    60661 ttatacatct tgataaatac cgctgccctt ccccaagcaa ataatgcata ctatttacat
    60721 catatataaa atttgcacct ggcacacagg gtaaatgctt tttcgaaatt tcagcggcct
    60781 ctatttgaga tttagcgatg ccaatctcgt taagattctc tgaaagatat aaatcacagg
    60841 tgtcaatctt acctgattta accaatggat aatccgttat actgtaggta tctttaaaag
    60901 ccacggcaaa catttcaata ataacaatta ctaccacaag cgaaatcaaa atgcgaaaaa
    60961 gaggaatact ccttttgcgc tctacttttt caacaacatc ttcttgtaaa tgagaatcat
    61021 cataagattg tttacttatt gttatgtttt cactgatgta aaagcctact ttgggtatcg
    61081 taagaatcac attttcttca acaccaatat ttttaaaact tttgcgtaac agggatatat
    61141 attggttcaa tgtgttgttt gacagatcaa atgaaaatct ctccaaaacg ctatcaatga
    61201 ttgagttcct gtcaaccgct ccaccattac tttccagtaa cactgccaaa atacaccccg
    61261 ccgtagctgg aaaatttatc tcatcctggg tatttacatt cgagagcgag catttgttcg
    61321 gatcaaaaat aatcgcgtca ttgataatga aaaaacatct ccgctccatg caggcaacaa
    61381 cacaaaataa gattttgtca ttttagccta ttcctggtgg aactgacaca agaaactaaa
    61441 atacaaatat aaccctgaaa aagtcatata aaacttatat ccacagaata aaaaatcaaa
    61501 catgtcagtt ttatcaatat aaagtatcgc aaggcgaaaa gtgatgcaag aatactccta
    61561 agtaaatgaa agcgcaccac atgaaattat caacacatgt aatttcatca ttaggtgcta
    61621 tcgaaaataa ctcaggagct acttaagatg aataatgtct tactcatgag acccgatgac
    61681 tcagaattta tgatacctga tgactggtca gggctaaacg caaaaggcat tacatacgaa
    61741 caagataata gagtgacaga gtcttttctg ccttttaaat ctttttattg ttcaccggtg
    61801 tgcacgacag ttgaactcat ctcataaaat aattgttttg ctatgggtat ccgctacctt
    61861 ctggaaaaga aaagagactt aaggctaaat cactttaaag ccttctctca taaatgcgag
    61921 acaaaacctg acgtgttaat tatgaccggg tgcaccgtcg ctgatactta tagtatattc
    61981 tcacgcattg ttgaagaaaa tataatgaaa acgatgacac ctatcgtcat cattactggg
    62041 catcactcag aagatctttg ttctctggca tcgaaactgg gaggagttaa aataatatca
    62101 ccgacagaaa agattgcaaa aatagaaaac gctatcaatg atgcttttaa cagcaagcct
    62161 aaaaaaataa gatttttaac agatatactc tctcctcaac aaattgagac catgaaaatg
    62221 gttgcgcagg gtagatccgt taatgagatt tcagccgcaa tgaatattag catcaagacc
    62281 gtgtatgcac ataaagttcg tgctatcaat aagctgggaa ttaagaaaaa agtttatgaa
    62341 gcgctttttt ataaagcgct aattcagttc aactgatgta aaaatcaccc tcgcttaata
    62401 aataatatat tctatgtctt tttttataag ttaaaaaagg cgggatatca aatcccgcca
    62461 tgaaattaac taatcatctg cattattctg cgtagatata agcctgggta cggatggtgt
    62521 aagagtctgg ctcaacaacc tgttctttca tatgggaaac gacgcgatag gcaccacctg
    62581 ttccagtatt tttcagcatc atataacgct taccgccaat ctcttcggag gcagctgaag
    62641 gaggatcctg ctcaagcgtt gtcagggaaa ctttcagttt atggtccggg ttattgtcac
    62701 cagaaattac tgcatccgct gaagtactgc tctccgcata aatatcatcc gggaaagtta
    62761 cagcatagtc gtggctctgc ccatcagatg cgccaacaat acctgttgcc aaaggcgtcc
    62821 atgcagttaa cgtaccgcta tatacccctt tcactggttg aatggtatta ttaacaacga
    62881 taggcttggt caaagtaaga ttagctgacg cttcagtacc ctggcttact aaagttcccg
    62941 cctgagaagc aaaagctgtc cccgccagca tgattgctaa caatgttttc ttcattatat
    63001 tttctccatg attttcattt acactgcaaa atgcagtttc tttctttgca tttacgtgct
    63061 atcgcgaaaa tgcttccacc aaataatgga aacatcttca caaaaataaa ttatgagttt
    63121 cttaatttag aaactataaa catgtcgccg ctatctggta taaccctgat gcacttttct
    63181 tcgcaggaag ttggtatttt gcgatacagg attgatcttt agaataaccc catttcacat
    63241 taaactcgcc ttccgcactc aagccagaaa cataagcaat accatcttca cctacaatgc
    63301 cggccatatt ttcagtgtct ttgctataac taatcacagc gccgaagggg atgggtttac
    63361 cctctttctg ttttaaggtt actaatcctt tatccccgct cttcactgcc aatgtcgcag
    63421 gcacaacggc tccagctgtc ggtacaacca attgactagt ttgtggtaag tcgtaattct
    63481 tacctatcgt tttttcatcc agaataacat cgtctcttcg gtatggctgc actggcagta
    63541 ttgcataccc acgaaaatca gtactcagtc ccggccagcc attaacgtta acccccgttg
    63601 ctccgggtgc ctctataagg gctaatgtcc catccacatt ttgaccaaat gtaagtccat
    63661 cccttgtaat aagtatccca ccgcttaaat ttgcacctag ctgtttcatg ttttcgctgg
    63721 agctgtaatg gctactaaac tgaccatatg atccattttt gccaacttgc acaatgttca
    63781 tcgtgctctt cggttgtgac tgatgagcgt tagttatatt tgtcgctaca tcccaccata
    63841 caccaccact attctgacct gataatgaaa cctgatagtt attaccctgc tctcccgaac
    63901 gactcatctg tgaagacgca taaatttgat tttcgtcacc cagcgataat ggcactgaaa
    63961 tacccacgct taatttccga tctactttcc atttatgcga tgtcagcaag tcgttttttg
    64021 cccagttaat ggagaatgtt atgcctctcc ataccgtact ataactcagg ctggtagacc
    64081 gcgtgtttcc tgtgccccgg gaagaattat acctcgtggt attgaagttc aaattcccga
    64141 tatt
//
LOCUS       DAJTYM010000009        63168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTYM010000009 DAJTYM010000000
VERSION     DAJTYM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            20..415
                     /locus_tag="OO575_000584"
     CDS             20..415
                     /locus_tag="OO575_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6663670.1"
                     /translation="MRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDNL
                     KKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKGS
                     DFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            651..1469
                     /gene="ybhA"
                     /locus_tag="OO575_000585"
     CDS             651..1469
                     /gene="ybhA"
                     /locus_tag="OO575_000585"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6663671.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1470..2528)
                     /gene="modC"
                     /locus_tag="OO575_000586"
     CDS             complement(1470..2528)
                     /gene="modC"
                     /locus_tag="OO575_000586"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6663672.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2531..3220)
                     /gene="modB"
                     /locus_tag="OO575_000587"
     CDS             complement(2531..3220)
                     /gene="modB"
                     /locus_tag="OO575_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6663673.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3220..3993)
                     /gene="modA"
                     /locus_tag="OO575_000588"
     CDS             complement(3220..3993)
                     /gene="modA"
                     /locus_tag="OO575_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6663674.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4160..4309)
                     /gene="acrZ"
                     /locus_tag="OO575_000589"
     CDS             complement(4160..4309)
                     /gene="acrZ"
                     /locus_tag="OO575_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6663675.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4438..5226
                     /gene="modE"
                     /locus_tag="OO575_000590"
     CDS             4438..5226
                     /gene="modE"
                     /locus_tag="OO575_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6663676.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5294..6766
                     /gene="modF"
                     /locus_tag="OO575_000591"
     CDS             5294..6766
                     /gene="modF"
                     /locus_tag="OO575_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6663677.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7027..8043
                     /gene="galE"
                     /locus_tag="OO575_000592"
     CDS             7027..8043
                     /gene="galE"
                     /locus_tag="OO575_000592"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6663678.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8053..9099
                     /gene="galT"
                     /locus_tag="OO575_000593"
     CDS             8053..9099
                     /gene="galT"
                     /locus_tag="OO575_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6663679.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9103..10251
                     /gene="galK"
                     /locus_tag="OO575_000594"
     CDS             9103..10251
                     /gene="galK"
                     /locus_tag="OO575_000594"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6663680.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10245..11285
                     /gene="galM"
                     /locus_tag="OO575_000595"
     CDS             10245..11285
                     /gene="galM"
                     /locus_tag="OO575_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6663681.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11488..12240
                     /gene="gpmA"
                     /locus_tag="OO575_000596"
     CDS             11488..12240
                     /gene="gpmA"
                     /locus_tag="OO575_000596"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6663682.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12407..13459)
                     /gene="aroG"
                     /locus_tag="OO575_000597"
     CDS             complement(12407..13459)
                     /gene="aroG"
                     /locus_tag="OO575_000597"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6663683.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13775..14155
                     /locus_tag="OO575_000598"
     CDS             13775..14155
                     /locus_tag="OO575_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6663684.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14269..15210
                     /gene="zitB"
                     /locus_tag="OO575_000599"
     CDS             14269..15210
                     /gene="zitB"
                     /locus_tag="OO575_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6663685.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15207..15926)
                     /gene="pnuC"
                     /locus_tag="OO575_000600"
     CDS             complement(15207..15926)
                     /gene="pnuC"
                     /locus_tag="OO575_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6663686.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15964..17007)
                     /gene="nadA"
                     /locus_tag="OO575_000601"
     CDS             complement(15964..17007)
                     /gene="nadA"
                     /locus_tag="OO575_000601"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6663687.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17256..17331)
                     /locus_tag="OO575_000602"
     tRNA            complement(17256..17331)
                     /locus_tag="OO575_000602"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17296..17298),aa:Lys,seq:ttt)
     gene            complement(17335..17410)
                     /locus_tag="OO575_000603"
     tRNA            complement(17335..17410)
                     /locus_tag="OO575_000603"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17375..17377),aa:Val,seq:tac)
     gene            complement(17462..17537)
                     /locus_tag="OO575_000604"
     tRNA            complement(17462..17537)
                     /locus_tag="OO575_000604"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17502..17504),aa:Lys,seq:ttt)
     gene            complement(17540..17615)
                     /locus_tag="OO575_000605"
     tRNA            complement(17540..17615)
                     /locus_tag="OO575_000605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17580..17582),aa:Val,seq:tac)
     gene            complement(17751..17826)
                     /locus_tag="OO575_000606"
     tRNA            complement(17751..17826)
                     /locus_tag="OO575_000606"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17791..17793),aa:Lys,seq:ttt)
     gene            complement(17991..18782)
                     /gene="cpoB"
                     /locus_tag="OO575_000607"
     CDS             complement(17991..18782)
                     /gene="cpoB"
                     /locus_tag="OO575_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6663688.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18792..19313)
                     /gene="pal"
                     /locus_tag="OO575_000608"
     CDS             complement(18792..19313)
                     /gene="pal"
                     /locus_tag="OO575_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6663689.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19348..20640)
                     /gene="tolB"
                     /locus_tag="OO575_000609"
     CDS             complement(19348..20640)
                     /gene="tolB"
                     /locus_tag="OO575_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6663690.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20773..22014)
                     /gene="tolA"
                     /locus_tag="OO575_000610"
     CDS             complement(20773..22014)
                     /gene="tolA"
                     /locus_tag="OO575_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6663691.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22079..22507)
                     /gene="tolR"
                     /locus_tag="OO575_000611"
     CDS             complement(22079..22507)
                     /gene="tolR"
                     /locus_tag="OO575_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6663692.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22511..23203)
                     /gene="tolQ"
                     /locus_tag="OO575_000612"
     CDS             complement(22511..23203)
                     /gene="tolQ"
                     /locus_tag="OO575_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6663693.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23200..23604)
                     /gene="ybgC"
                     /locus_tag="OO575_000613"
     CDS             complement(23200..23604)
                     /gene="ybgC"
                     /locus_tag="OO575_000613"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6663694.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23754..24047)
                     /gene="ybgE"
                     /locus_tag="OO575_000614"
     CDS             complement(23754..24047)
                     /gene="ybgE"
                     /locus_tag="OO575_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6663695.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24047..24160)
                     /gene="cydX"
                     /locus_tag="OO575_000615"
     CDS             complement(24047..24160)
                     /gene="cydX"
                     /locus_tag="OO575_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6663696.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24175..25314)
                     /gene="cydB"
                     /locus_tag="OO575_000616"
     CDS             complement(24175..25314)
                     /gene="cydB"
                     /locus_tag="OO575_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6663697.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25330..26898)
                     /gene="cydA"
                     /locus_tag="OO575_000617"
     CDS             complement(25330..26898)
                     /gene="cydA"
                     /locus_tag="OO575_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6663698.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27747..30380)
                     /gene="mngB"
                     /locus_tag="OO575_000618"
     CDS             complement(27747..30380)
                     /gene="mngB"
                     /locus_tag="OO575_000618"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6663699.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30398..32314)
                     /gene="mngA"
                     /locus_tag="OO575_000619"
     CDS             complement(30398..32314)
                     /gene="mngA"
                     /locus_tag="OO575_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6663700.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32484..33206
                     /gene="mngR"
                     /locus_tag="OO575_000620"
     CDS             32484..33206
                     /gene="mngR"
                     /locus_tag="OO575_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6663701.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33309..34178)
                     /gene="sucD"
                     /locus_tag="OO575_000621"
     CDS             complement(33309..34178)
                     /gene="sucD"
                     /locus_tag="OO575_000621"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6663702.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34178..35344)
                     /gene="sucC"
                     /locus_tag="OO575_000622"
     CDS             complement(34178..35344)
                     /gene="sucC"
                     /locus_tag="OO575_000622"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6663703.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35438..36655)
                     /gene="odhB"
                     /locus_tag="OO575_000623"
     CDS             complement(35438..36655)
                     /gene="odhB"
                     /locus_tag="OO575_000623"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6663704.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36670..39471)
                     /gene="sucA"
                     /locus_tag="OO575_000624"
     CDS             complement(36670..39471)
                     /gene="sucA"
                     /locus_tag="OO575_000624"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6663705.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39772..40488)
                     /gene="sdhB"
                     /locus_tag="OO575_000625"
     CDS             complement(39772..40488)
                     /gene="sdhB"
                     /locus_tag="OO575_000625"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6663706.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40504..42270)
                     /gene="sdhA"
                     /locus_tag="OO575_000626"
     CDS             complement(40504..42270)
                     /gene="sdhA"
                     /locus_tag="OO575_000626"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6663707.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42270..42617)
                     /gene="sdhD"
                     /locus_tag="OO575_000627"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42270..42617)
                     /gene="sdhD"
                     /locus_tag="OO575_000627"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6663708.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42611..43015)
                     /gene="sdhC"
                     /locus_tag="OO575_000628"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42611..43015)
                     /gene="sdhC"
                     /locus_tag="OO575_000628"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6663709.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43396..43503
                     /locus_tag="OO575_000629"
     CDS             43396..43503
                     /locus_tag="OO575_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663710.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43709..44992
                     /gene="gltA"
                     /locus_tag="OO575_000630"
     CDS             43709..44992
                     /gene="gltA"
                     /locus_tag="OO575_000630"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6663711.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45383..45949
                     /locus_tag="OO575_000631"
     CDS             45383..45949
                     /locus_tag="OO575_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6663712.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46000..48459
                     /locus_tag="OO575_000632"
     CDS             46000..48459
                     /locus_tag="OO575_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6663713.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48474..49205
                     /locus_tag="OO575_000633"
     CDS             48474..49205
                     /locus_tag="OO575_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6663714.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49202..50278
                     /locus_tag="OO575_000634"
                     /pseudo
     CDS             49202..50278
                     /locus_tag="OO575_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50430..51476
                     /locus_tag="OO575_000635"
     CDS             50430..51476
                     /locus_tag="OO575_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6663715.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51473..52264)
                     /gene="nei"
                     /locus_tag="OO575_000636"
     CDS             complement(51473..52264)
                     /gene="nei"
                     /locus_tag="OO575_000636"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6663716.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52300..53034)
                     /gene="pxpA"
                     /locus_tag="OO575_000637"
     CDS             complement(52300..53034)
                     /gene="pxpA"
                     /locus_tag="OO575_000637"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6663717.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53024..53956)
                     /gene="pxpC"
                     /locus_tag="OO575_000638"
                     /pseudo
     CDS             complement(53024..53956)
                     /gene="pxpC"
                     /locus_tag="OO575_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(53950..54606)
                     /gene="pxpB"
                     /locus_tag="OO575_000639"
     CDS             complement(53950..54606)
                     /gene="pxpB"
                     /locus_tag="OO575_000639"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6663718.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54629..55372)
                     /gene="ybgI"
                     /locus_tag="OO575_000640"
     CDS             complement(54629..55372)
                     /gene="ybgI"
                     /locus_tag="OO575_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6663719.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55643..57124
                     /gene="dtpD"
                     /locus_tag="OO575_000641"
     CDS             55643..57124
                     /gene="dtpD"
                     /locus_tag="OO575_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6663720.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57274..58692)
                     /gene="phrB"
                     /locus_tag="OO575_000642"
     CDS             complement(57274..58692)
                     /gene="phrB"
                     /locus_tag="OO575_000642"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6663721.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58689..59198)
                     /locus_tag="OO575_000643"
     CDS             complement(58689..59198)
                     /locus_tag="OO575_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6663722.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59327..59614
                     /locus_tag="OO575_000644"
     CDS             59327..59614
                     /locus_tag="OO575_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6663723.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59669..60049)
                     /locus_tag="OO575_000645"
     CDS             complement(59669..60049)
                     /locus_tag="OO575_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663724.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60052..61584)
                     /locus_tag="OO575_000646"
     CDS             complement(60052..61584)
                     /locus_tag="OO575_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCR6663725.1"
                     /translation="MTLHRQPGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAW
                     DLAQRRLCVTDGLGRTRYHQWDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGG
                     KWRYVYDLQGHITETHDPLGRVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TENKTKAGSYHWDDIKDPSVTKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPW
                     ET"
BASE COUNT        15404 a        17006 c        15422 g        15336 t
ORIGIN      
        1 caaaggagat ctggcggcta tgagatggga ggatataaga gatagcaggc tatacgtgga
       61 acaacaaaaa acaggggcaa aaattcgcat ttctctcccc accacgattt ccagattgaa
      121 tttaacactt gccgatgtac tggataactt gaaaaaaata aacggtaaaa acgaaaagtt
      181 actcggaggg aagacagcca ggacaattgc agcacagttc aggattgccc gagacacatc
      241 cggattgaaa tgggaaggtg acccaccgcc atttcatgaa atccgaagtt tatccggtcg
      301 cctgcactca gcagaaaaag gcagtgattt cactcaggcg ctccttggac atcgttcatc
      361 cagcatgacg gataaatacc gtgacggacg agggagggaa tggaaagaca tttagaatta
      421 atttcgaatg atttcgaaat gccacttgta attaattgat tataaagaat taattcctaa
      481 agagcatttg cttcatgaat gctcctttgc atttagcaac tgactgtaac agctaaaatt
      541 agtcgctttt ggcggtgaat gctgaattaa aacgcagaat tttgtccgat agtggtatcg
      601 gtgtaccatt cgcgcagacg aaaattctta accttaatct ggaatacgcc atgaccacac
      661 gcgtgattgc tctcgactta gacggcacct tattgacccc gaaaaagacc ttgcttccat
      721 catcgataga agccctggcc cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag
      781 gtcgccatca cgtcgctatt catccttttt atcaggcgct ggcgctggat acacctgcta
      841 tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa aaccgtgctg gaagcggacc
      901 caatgcccgt taataaagcc ctgcaactca ttgagatgct gaatgaacac cacattcacg
      961 gtctgatgta tgtcgatgat gcaatggtct atgagcaccc gaccgggcat gtcattcgca
     1021 cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc gactttcaca caagtcgctt
     1081 ctctggctga aacgacgcaa caagttaacg ccgtatggaa gttcgccctg acgcacgatg
     1141 acctgccgca attgcagcat tttggtaagc atgtcgaaca tgaactggga ctggagtgtg
     1201 aatggtcctg gcacgatcag gttgatattg cacacggcgg caacagcaaa ggtaaacgtt
     1261 tgacgaaatg ggttgaggcg caaggttggt cgatggaaaa cgtcgtggca ttcggcgata
     1321 actttaatga tatcagtatg ctggaagccg ctggtacagg cgtggcgatg ggcaacgccg
     1381 atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc gacagcattg
     1441 cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact tttaatttgc
     1501 gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg gctgatacgc
     1561 gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt gtcgtcataa
     1621 ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg cggttgtaaa
     1681 accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag cggttcgccc
     1741 agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc gtaatgcgga
     1801 tgatgctcaa gcaccgtcac tttcagaatg ctactttgtt gctctttcgg cagccacgga
     1861 ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac ctgaccgttt
     1921 tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg gctgacatac
     1981 aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag ttcgcgttta
     2041 cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg tgctgtcagc
     2101 aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc tggtaaacgg
     2161 tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc gaccatactt
     2221 ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacaa ccgcgcatcc
     2281 tgaaaaacat agccaacacg acgcttttcc ggcgtcaggc agataccttt ttcggcatca
     2341 tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag tccactgatg
     2401 gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt gatgccattg
     2461 gcgggcagcg tttcattaat ggtcaggcaa tggttgccca acgtctggga aaaattcagc
     2521 tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat tctgaaatca
     2581 acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg ccactttccc
     2641 cgccgggggt ctggatcagg gtatacatgg cagaaggaat ggttcgcgtt tcgctaggaa
     2701 tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca aaagccagta
     2761 ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag aaaacgcgcc
     2821 agcgcccggc acctagtgtt cttgcggcct gttccagttt gacatcaacc ccttccagcg
     2881 ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg agaaccgcgc
     2941 cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca ccgataaatc
     3001 cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt aacaccagcg
     3061 gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc accagtaacc
     3121 aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa actttcaggc
     3181 ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg taaatccgta
     3241 acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg ctttcactgt
     3301 cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat gtgaatcttc
     3361 cgggaaggtg gcaaccactt ttaccccttt gctggcaact gcgtcagaac cgtagacaat
     3421 gcccagaggc gcttcgttac gttcgaccag cgccagcgcc ccacgaacat cttccgctgg
     3481 ggccagtttc ggagagagcg tatcccatgc gcccagtttt tgcagtgctt cttttgcata
     3541 aatgccagcg ggaacatgtt ccggatcgcc aaccgccagg cgaccgccat tcagcagcga
     3601 agtccagtta gttttgctgt cgatggtgaa atctttctgc tcgctggctt tcggtgctac
     3661 aacgaccagg ctattgccga gcagtgtctg acgcgtagca gtatcgatcg cttttttatc
     3721 aaccgcataa tccatccatt tctgatcggc agaaataaac agatccgcag gggcacccgc
     3781 ttcaatctga cgggcgagag tagatgacga agcgaaagaa gaaaccacat ccacgccttt
     3841 ctctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg cggcggcgaa
     3901 caccgtgatt ttcccttcat ctgccagtgc attgccagca actgcgaaag agagtgccgc
     3961 cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt aacgttatgt
     4021 aggcgacaat ataacgacta actcggaatt ttcccagcag ttattgctaa cctttgtgag
     4081 gtagataaga aaaaatatcg gcaggaaaag caggaagttg agaaaaagaa aatgcccgac
     4141 taagcgggca ctcagggaat caatgatttt gtccgggctg gtctttttta ccaacaccag
     4201 aaaagatgtt gaatacttca ccaagaccgt aaatcagacc caggatgatg gccatcacga
     4261 caggtaccat gattacggcg aataccagac tttttaataa ctctaacatg gtcaactcca
     4321 gatatagtca tgagactatt ctaaccgcta agcacagaaa agcactcccc ttttgtgcgg
     4381 tcagctttgc gtggcgttcg ttttccgtca caataagact tttgccagga cattgttatg
     4441 caggccgaaa tccttctcac ccttaagctc caacaaaaat tattcgccga cccgcgccgc
     4501 atttcgctac taaaacacat tgcgctttcc ggttccatta gccagggagc gaaagatgcc
     4561 ggtatcagct ataaaagcgc ctgggatgcc attaacgaga tgaatcagtt aagtgagcat
     4621 attctggtcg agcgcgcaac aggcggtaaa ggtggcggag gcgcagtact gacccgctat
     4681 ggtcagcgac tgattcagct ctatgactta ctggcgcaaa tccagcaaaa agcctttgat
     4741 gtgttaagtg acgatgacgc cctgccgctg aacagcctgc tggccgcgat ctcacgtttt
     4801 tcactgcaaa ccagcgcccg taaccagtgg ttcggtacca tcaccgcccg cgatcatgat
     4861 gacgttcaac agcatgttga tgtcttactg gctgacggaa aaacacgcct gaaagtcgca
     4921 attaccgcac aaagcggcgc acgtctgggg ctggatgaag gcaaagaagt gttgatattg
     4981 ctaaaagcgc cgtgggtagg cattactcag gacgaagcgg tcgcacaaaa cgccgacaac
     5041 caattaccgg gtattattag tcatattgag cgcggcgcag agcagtgcga agtattaatg
     5101 gcgctacccg acgggcaaac actgtgcgcc acagtgccgg taaatgaagc gacttctctt
     5161 cagcaagggc agaatgtcac ggcctacttt aatgccgaca gcgtgattat cgccacgctg
     5221 tgctaagcgt gttgacaatt tgttatgaaa cacgtatccc tgtcagtaat cgctgcacaa
     5281 aatgggatat aaaatgtcat cgttgcaaat tttgcaaggc acgtttcgtc ttagcgacac
     5341 aaaaacgctg caattgcctc agctaacgtt aaacgcgggt gatagttggg cgtttgtcgg
     5401 ttcgaatgga agtggaaagt cggcgctggc ccgcgcgctg gcgggggaac ttccgctttt
     5461 gaaaggtgaa cggcaaagcc agttttccca catcactcgt ctctccttcg agcaattgca
     5521 aaagctcgtc agcgacgaat ggcaacggaa taacaccgat atgctcggcc ctagcgaaga
     5581 ttacaccgga cgcactacgg ctgagattat tcaggatgaa gtaaaggatg caccgcgttg
     5641 catgcaactg gcgcagcagt tcggtattac cgccctcctc gaccgacgct ttaaatacct
     5701 ttccactggc gagacgcgaa aaaccctgct gtgtcaggcg ctgatgtcgg agcctgactt
     5761 gttgattctt gatgagccgt tcgatggcct ggatgttgcc tcacgtcagc agctggctga
     5821 gcgactcgcc tcgttacatc agtccggtat tactttggta ctggtgctca atcgcttcga
     5881 taagatcccg gaatttgtcc agtttgctgg cgtgctggcg gattgtacgt tagcggaaac
     5941 tggcgctaaa gaggaactgc tccagcaagc actcgtcgcg caacttgcgc atagtgaaca
     6001 gcttgaaggt gtgcaactgc cggagccgga tgaaccttca gcacgtcacg ccttacccgc
     6061 caacgaatcg cgcattgtgc tgaacaatgg cgtggtttct tataatgatc gccccattct
     6121 taataacctt agctggcagg tgaatccagg cgaacactgg caaattgtcg ggccaaatgg
     6181 cgcgggaaaa tcgacgttat taagcctgat tactggcgat catccgcaag gttacagcaa
     6241 cgatttgacg cttttcggcc gacgtcgcgg cagcagcgaa accatctggg acatcaaaaa
     6301 gcatatcggt tacgtcagca gtagtttgca tctggattac cgggtcagca ctaccgtgcg
     6361 taatgtgatt ctttctggct attttgattc gattggcatt tatcaggtcg tttcggatcg
     6421 ccagcaaaaa ctggtgcagc agtggctgga tattctcggc attgataaac acacggctga
     6481 cgctccgttc catagtcttt cctggggaca gcagcgtttg gcgctgatcg tccgcgcact
     6541 ggtgaaacat ccgacgttgc ttattctcga tgaaccacta caggggcttg atccgctcaa
     6601 tcgccaactt atccgccgtt ttgttgatgt gctgattagc gaaggtgaaa cgcaattgtt
     6661 gtttgtttcg caccacgctg aagatgcgcc tgcctgtatt acccatcgcc ttgagttcgt
     6721 gccggacggt ggactctatc gctatgtgct gacaaaaata tattgagtcg gtagtgctga
     6781 ccttgccgga ggcggcctaa gcgcactctc cggccaacgg ttcgacgcat gcaggcatga
     6841 aaccgcttct tttttcagat aaaaagcgca atcattcata aaccctctgt tttataatca
     6901 cttaatcgcg cataaaaaac ggctaaattc ttgtgtaaac gattccacta atttattcca
     6961 tgtcacactt ttcgcatctt tgttatgcta tggttatttc ataccataag cctaatggag
     7021 cgaattatga gagttctggt taccggtggt agcggttaca ttggaagtca tacctgcgtg
     7081 caattactgc aaaacggtca tgatgtcatc attcttgata acctctgtaa cagtaagcgc
     7141 agcgtactgc ctgttatcga gcgtttaggc ggcaaacatc caacgtttgt tgaaggcgat
     7201 attcgtaacg aagcgttgat gaccgagatc ctgcacgatc acgctatcga caccgtgatc
     7261 cacttcgccg ggctgaaagc cgtcgccgaa tcggtacaaa aaccgctgga atattacgac
     7321 aacaatgtca acggcactct gcgcctgatt agcgccatgc gcgccgctaa cgtcaaaaac
     7381 tttattttta gctcctccgc caccgtttat ggcgatcagc cccaaattcc atacgttgaa
     7441 agcttcccga ccggcacacc gcaaagccct tatggcaaaa gcaaattgat ggtggaacag
     7501 atcctcaccg acctgcaaaa agcccagccg gactggagca ttgccctgct gcgctacttc
     7561 aacccggttg gcgcgcatcc gtcgggcgat atgggcgaag atccgcaagg cattccgaat
     7621 aacctgatgc catacatcgc ccaagttgct gtaggccgtc gcgactcgct ggcgattttt
     7681 ggtaacgatt atccgaccga agatggtact ggcgtacgcg attacatcca cgtaatggat
     7741 ctggcggacg gtcacgtcgt ggcgatggaa aaactggcga acaagccagg cgtacacatc
     7801 tacaacctcg gtgctggcgt aggcagcagc gtgctggacg tggttaatgc cttcagcaaa
     7861 gcctgcggca aaccggttaa ctatcatttt gcaccgcgtc gcgaaggcga ccttccggcc
     7921 tactgggcgg acgccagcaa agccgaccgt gaactgaact ggcgcgtaac gcgcacactc
     7981 gatgaaatgg cgcaggacac ctggcactgg cagtcacgcc atccacaggg atatcccgat
     8041 taaggaacga ccatgacgca atttaatccc gttgatcatc cacatcgccg ctacaacccg
     8101 ctcaccgggc aatggattct ggtttcaccg caccgcgcta agcgcccctg gcagggggcg
     8161 caggaaacgc cagccaaaca ggtgttacct gcgcacgatc cagattgctt cctctgcgca
     8221 ggtaatgtgc gggtgacagg cgataaaaac cccgattaca ccgggactta cgttttcact
     8281 aatgactttg cggccttgat gtctgacacg ccagatgcgc cagaaagcaa cgatcctttg
     8341 atgcgttgcc agagcgcgcg cggtaccagc cgggtgatct gcttttcacc ggatcacagt
     8401 aaaacgttgc cagaactgag cgttgcggca ttgacggaaa tcgtcaaaac ctggcaggag
     8461 caaaccgcag agctgggaaa aacgtacccg tgggtgcagg tctttgaaaa caaaggtgcg
     8521 gcgatgggct gctctaaccc gcatccgcac ggacagattt gggcaaatag cttcctgcct
     8581 aacgaagctg agtgcgaaga ccgcctgcaa aaagaatatt ttgccgaaca gaaatcacca
     8641 atgctggtgg attatgttca gcgcgagctg gcagacggta gccgtaccgt tgtcgaaacc
     8701 gaacactggt tagccgttgt gccttactgg gctgcctggc cgttcgaaac gctactgctg
     8761 cccaaagccc acgttttgcg gatcaccgat ttgaccgacg cccagcgcag cgatttggcg
     8821 ctggcgttga aaaagctgac cagtcgttat gacaacctct tccagtgctc cttcccctac
     8881 tctatgggct ggcacggcgc accatttaat ggcgaagaga atcaacactg gcagctgcac
     8941 gcgcactttt atccgcctct gctgcgctcc gccaccgtac gtaaatttat ggttggttat
     9001 gaaatgctgg cagagaccca gcgagacctg accgcagaac aggcagcaga gcgtttgcgc
     9061 gcagtcagcg atatccattt tcgcgaatcc ggagtgtaag aaatgagtct gaaagaaaaa
     9121 acacaatctc tgtttgccaa cgcatttggc taccctgcca ctcacaccat tcaggcgcct
     9181 ggccgcgtga atttgattgg tgaacacacc gactacaacg acggtttcgt tctgccctgc
     9241 gcgattgatt atcaaaccgt gatcagctgt gcaccacgcg atgaccgtaa agttcgcgtg
     9301 atggcagccg attatgaaaa tcagctcgac gagttttccc tcaatgcgcc cattgtcgcg
     9361 catgaaaact atcaatgggc gaactacgtt cgtggcgtgg tgaaacatct gcaactgcgt
     9421 aacaacagct tcggcggtgt ggacatggtg atcagcggca atgtgccgca gggtgccggg
     9481 ttaagttctt ccgcttcact ggaagtcgcg gtcggaaccg tattgcagca gctttatcat
     9541 ctgccgctgg acggcgcaca aatcgcgctt aacggtcagg aagcagaaaa ccagtttgtt
     9601 ggctgtaact gcgggatcat ggatcagcta atttccgcac tcggcaagaa agatcatgcc
     9661 ttgctgattg actgccgctc actggggact aaagcagttt ccatgccgaa aggtgtggct
     9721 gtcgtcatca tcaacagtaa cttcaaacgt accctggttg gcagcgaata caacacccgt
     9781 cgtgaacagt gcgaaaccgg tgcgcgtttc ttccagcagc cagctctgcg cgatgtcacc
     9841 attgaagagt tcaacgctgt tgcacatgag ctggacccaa tcgtggcgaa acgcgtgcgt
     9901 catatcctga ctgaaaacgc ccgcaccgtt gaagctgcca gcgcgctgga gcaaggcgac
     9961 ctgaaacgta tgggcgagtt gatggccgag tctcatgcct ctatgcgcga tgatttcgaa
    10021 atcaccgtgc cgcaaattga cactctggta gaaatcgtca aagctgtgat tggcgacaaa
    10081 ggtggcgtac gcatgaccgg cggcggattt ggcggctgta tcgtcgcgct gatcccggaa
    10141 gagctggtgc ctgccgtaca gcaagctgtc gctgaacaat atgaagcaaa aacaggtatt
    10201 aaagagactt tttacgtttg taaaccatca caaggagcag gacagtgctg aacgaaactc
    10261 ccgcactggc acccgatggt cagccgtacc gactgttaac tttgcgtaac aacgcaggga
    10321 tggtagtcac gctgatggac tggggtgcga ctttactttc cgcccgtatt ccgctttccg
    10381 atggcagcgt ccgcgaggcg ctgctcggct gtgccagtcc ggaatgctat caggatcagg
    10441 ctgcgtttct gggggcctct attggtcgtt atgccaaccg catcgccaat agtcgctata
    10501 cctttgacgg tgaaaccgtg acgctttcgc caagccaggg cgttaaccag ctgcacggcg
    10561 gcccggaagg gttcgacaaa cgtcgctggc agattgtgaa ccagaacgat cgtcaggtgc
    10621 tgtttgccct gagttcagat gatggcgatc agggcttccc gggtaatctc ggcgcgacgg
    10681 tgcaatatcg tctgaccgac gataaccgta tctccattac ttatcgcgcc acagttgata
    10741 aaccttgccc ggtgaatatg actaatcacg tctatttcaa tcttgacggc gagcagtctg
    10801 acgtgcgcaa tcacaagttg cagattctgg cggacgaata tcttccggtt gatgaaggcg
    10861 gcattccgca cgacggcttg aaatctgtcg ccggaacgtc ttttgatttc cgcagcgcca
    10921 aaatcatcgc cagtgagttt cttgccgacg acgatcagcg caaagtgaaa ggttacgatc
    10981 acgcattctt gttacaggcc aaaggcgatg gcaagaaagt ggcggcgcat gtctggtcag
    11041 cagatgaaaa attgcagctg aaggtctaca ccaccgctcc ggctcttcaa ttctactccg
    11101 gcaacttcct cggcggcaca ccgtcgcggg gaaccgaacc ttacgccgac tggcaagggc
    11161 tggcgttgga aagcgagttt ctgccggaca gcccgaacca ccctgaatgg ccgcaaccgg
    11221 actgcttcct gcgtcctggc gaagagtatt ccagcctgac ggaatatcag tttattgctg
    11281 agtaatgtta agccctcgtc atgagggctt tatcttcata ttgttcaaat caccagcaaa
    11341 caccgacata tttgcaactc aatattcaca acaaccttac actgcgccac tattttcgct
    11401 atggttatgc gtaagcattg ctgttgcttc gtcacggcaa tataatgaga attattatca
    11461 ttaaaagatg atttgaggag taagcatatg gctgtaacta agctggttct ggttcgtcat
    11521 ggcgaaagtc agtggaacaa agaaaaccgt ttcaccggtt ggtacgacgt ggatctgtct
    11581 gagaaaggcg taagcgaagc aaaagcagca ggtaagctgc tgaaagagga aggttacagc
    11641 tttgactttg cttacacttc tgtgctgaaa cgcgctatcc ataccctgtg gaatgtgctg
    11701 gacgaactgg atcaggcatg gctgcccgtt gagaaatcct ggaaactgaa cgaacgtcac
    11761 tacggtgcgt tgcagggtct gaacaaagcg gaaactgctg aaaagtatgg cgacgagcag
    11821 gtgaaacagt ggcgtcgtgg ttttgcagtg actccgccgg aactgactaa agatgatgag
    11881 cgttatccgg gtcacgatcc gcgttacgcg aaactgagcg agaaagaatt gccgctgacg
    11941 gaaagcctgg cgctgaccat tgaccgcgtg atcccttact ggaatgaaac cattctgccg
    12001 cgtatgaaga gcggtgagcg cgtaatcatc gccgcacacg gtaactccct gcgtgcgctg
    12061 gtgaaatatc ttgataacat gagcgaagaa gagattcttg agctgaatat cccgactggc
    12121 gtgccgctgg tgtatgagtt cgacgagaat ttcaaaccgc tgaaacgcta ttatctgggt
    12181 aatgctgacg agatcgcggc gaaagcagca gccgttgcga accagggtaa agcgaagtaa
    12241 acgtcattcg tttaaaaaat gagaaagccg actgcaagtg agtcggcttt tttgtttgtc
    12301 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    12361 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    12421 cttttactgc attcaccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    12481 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    12541 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    12601 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgaa ttagcatggc
    12661 tgaaatcgat catcacctga gctggcagac ctgctttgtt cagcccttct ttcacttcag
    12721 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcgc
    12781 cgttaccact ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12841 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12901 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12961 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    13021 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    13081 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    13141 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    13201 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    13261 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    13321 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttgcgg
    13381 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    13441 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    13501 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    13561 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    13621 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    13681 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13741 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13801 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13861 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13921 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13981 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga
    14041 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaac
    14101 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    14161 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    14221 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    14281 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    14341 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    14401 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    14461 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    14521 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    14581 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg aggcatgatg
    14641 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    14701 ggcagcgaag agaaaaacct caacgtacga gcagcggcac tgcatgtgct cggggacctg
    14761 ctgggttcgg ttggggctat tattgccgcg ttgattatta tctggactgg ctggacacct
    14821 gctgacccca ttctctcgat actggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14881 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14941 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    15001 tggatggtag gcgagaagcc ggtgatgacg ttgcatgtgc aggtgatccc gccacacgat
    15061 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    15121 accaccattc agatggaata tcaaccttgt cataggccag actgccacct gaatgaaggg
    15181 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    15241 gcgctgttga tccacatccg gctgccgttg agcgcaataa aggtcaggat gatgtactcc
    15301 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    15361 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    15421 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    15481 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    15541 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagt
    15601 gccttcggca atggcaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    15661 gaccacgcat accagccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15721 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg taacgttgat caggccaaag
    15781 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15841 cctaccgctt cgatccatga gagatcataa ccgcccgccc ctattggtat atgtaccagg
    15901 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15961 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    16021 ctctcgcagc ctctcatcga catgaacctc gtgattactt ccttcctgtt ctaatgcctc
    16081 tgcgatagcc tgaaggtcat tcatggccat ccacggacaa tgcgcgcagc tgcggcaggt
    16141 tgcgccctca cccgcggttg gtgcttccag taactcttta tctggcaccg cctgctgcat
    16201 tttgtagaaa ataccccgat cggttgccac aataagcctc tgatgaggca atgcttttgc
    16261 agcagcgatc agttgactgg tggaaccgac cgcatccgcc atatcgacaa tagcttgtgg
    16321 tgattctgga tgcaccagta tggcagcatc cgggtattct tcttgcaagc gggttaacgc
    16381 ctgagtctta aattcatcat gcacaatgca ggcaccctgc cagcataaaa tgtctccacc
    16441 cgtctgtttt tgcacgtaac gccccagatg tttgtcgggt gcccagatga ttttttcacc
    16501 caaactatca agatgatcaa taagttcgac ggcaatgctt gaagttacca cccaatctgc
    16561 gcgcgctttt accgcagcag aagtgttggc gtagacgacg acagtacgat cgggatgggc
    16621 atcgtaaaat gcgttaaatt cttcaacagg gcagccgaga tccagtgaac actcaacctg
    16681 aagtgtcggc atcagaattg ttttttccgg actgagaatt ttggcggttt ctcccataaa
    16741 tctcacccca gcgactaaca aagtagaagc gggatgcttt gcaccgaagc gcgccatttc
    16801 cagagaatca gaaatacagc caccggtttc ttctgccagt tgttgaattt cgggatcggt
    16861 atagtagtgg gcaaccatca ccgcattacg ttcttttagc agacgtttta tcttctcgcg
    16921 gtaatacgct ttttcatcaa tgcttaacgg cgtcggcttc ggggggaaag gataaatcgc
    16981 cgtgtctgga tcaaacatta cgctcatctt accatctcgt tttacaggct taacgttaaa
    17041 accgacatta gcgtaacatt cgctgttatc ggtgtaattt gttttatatg ctaaacaaga
    17101 tagtcgaatg gtggggaaaa gtcactcgaa ttacgcctaa ttccaccgac gtagagttgc
    17161 ttgcacagta agctctgacg atgtcatcag gtttacaaag tgaaattgag gatgcctgaa
    17221 tagcaaaaaa gcgcctttag ggcgcttttt tgcattggtg ggtcgtgcag gattcgaacc
    17281 tgcgaccaat tgattaaaag tcaactgctc taccaactga gctaacgacc cgagtggtgg
    17341 gtgatgacgg gatcgaaccg ccgaccccct ccttgtaagg gaggtgctct cccagctgag
    17401 ctaatcaccc gatactgcgc tggatactac aaactacttc caccgaaccc accgtaatta
    17461 gtggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc
    17521 aactgagcta acgacccggt ggtgggtgat gacgggatcg aaccgccgac cccctccttg
    17581 taagggaggt gctctcccag ctgagctaat cacccgacac tacgctggat actacaacta
    17641 actatgttag tgggggtcgt gcaggatgac tcgacttcgt ctcgcccttc ggcgccgttg
    17701 ctgatgcaac gttatccttc acgttttact accgcttcca ccttagcgat tggtgggtcg
    17761 tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa
    17821 cgacccactt tttcgttgct ttcggtttgt ttgatatccc gtggcaacgg cggcatatat
    17881 tactgatttc agacttgagc gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga
    17941 ttcgcacgac acgaccagaa ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg
    18001 ttcagacgtt tttgtgcctg tttagcgcca tcagtaccag ggtatttact gataacctgc
    18061 tggtatacgg ctttcgcttt tgcggtgtca cctttgtcct gcatgatgac gccgacttta
    18121 aacatcgcat cagcagcctt tggtgacttc ggatagtttt tcactaccga agcaaaatag
    18181 tacgccgcat catctttttt acccttgttg tagtttaact gacccagcca ataattggcg
    18241 tttggcaggt aagttgaatc agggtaattt ttgatgaaat tctgaaatgc caccattgcg
    18301 tcatcctggc gggatttatc ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca
    18361 tcgccgcttt tcaccggcgc gccagcattc gcagtaccag catcagctgt cggcgtcgtt
    18421 gatgccgtcg caccgctttg atcgctgctg gttgattgcg ccgctgcacc accgctgctg
    18481 aggctgtcga tctgcaacag gatctgcttt tgccgctcca cgacctgatt cagttgatac
    18541 tgattttcct gaatctgacc acgcagggaa tcaatatcgg attgattatc agaaagttgt
    18601 tgctggagtt gggttaaaag ctggctgtga gcattagaaa tacgctcaag ttgagtgacg
    18661 cggtcttcga ccgagcctga gctgacacta ctgattggtg cctgagcaaa agcggcccag
    18721 ggggccgcta taccaaccag taacgacaga ctcaatagtt gatgtctgaa gttactgctc
    18781 atgcaattct cttagtaaac cagtaccgca cgacggtttt tggagtatgc cgcttcgtca
    18841 tgacccagta ctgcaggttt ttctttaccg taagaaacga tggagatctg gtctgcagaa
    18901 acgcctttac cctgcaggta catcttaacg gcgttcgcac gacgttcacc cagggagatg
    18961 ttgtattccg gagtaccacg ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg
    19021 ttgctacgca ggaagtttgc atgtgcatcc agcatttgag cgaagtcaga acggatatcg
    19081 tacttgtcca gatcgaagta aacgatgttg ttctgctgca gctgttgcat ttgcagacga
    19141 gcctgctctt cggaagacat gttgccgttg ccgccgttcg catccatacc agtgccggca
    19201 cccagcatgc cttcgctgcc gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt
    19261 gccataacag gcagagcaat catcagccct ttcagcactt tgttcagttg catttcaatg
    19321 attcctttac tattcaatta attattatca cagatacggc gaccaggcag ggaatttgac
    19381 ctgtccatca gttgccggaa gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag
    19441 cacggatccc atcccctgag aagagctgta gattaccata gtgccgttag gtgccagact
    19501 tggcgtttca tccaggaacg tggacgacag aacttgtacg cctcccgttg ccagatcttg
    19561 tttggcaatg tgctgctgcc caccattgga gctgaccatt accataaatt taccgtcgct
    19621 gctgacatcc gcatcctggt tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc
    19681 gttgatattc actttataaa cctgcggacg accggcctgg tcagaagtaa atgccaggtt
    19741 ctggctatcc gggaaccagg tcggttcggt attgttactg cgaccatcag tcacctggcg
    19801 gatctgacca gaagccaaat ccattacgta caggttcaga ctaccggttt tcgacaaggc
    19861 gaatgccagt ttgctgccgt ctggcgagaa tgcaggtgca ccgttgtgac gcgggaatga
    19921 agccacctga cgtacagcgc catttgccag cgtctgaata accagcgcgg aacgaccgct
    19981 ttcgaaggtc acataagcca gtttagaacc gtctggtgac cacgccggag acatcagcgg
    20041 ctgcggtgaa cggtgaacga caaactggtt gtaaccgtca tagtcagata cgcgcagttc
    20101 atacgggaac tgaccgccgt tggtctgaac aacgtaggca atacgggtac ggaacgcacc
    20161 tttaatgccg gtcagttttt caaacacttc atcactggcg gtatgaccag cataacgcag
    20221 ccactgcttg ttcactttgt acgagttctg agcaagtaca gtacccggtg cgccgccagt
    20281 gtcaacaagt tgataagcaa cattgtaaga accatccgga ttcggagtga cttgaccgac
    20341 aactacagcg tcaatgccca gtgcggacca tgcagctggt tgtacttcct gcgcactacc
    20401 cggctgctgt ggcagacgag cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc
    20461 agcagcaacg atgccgccaa tatcttcagg tgccgcacca ggccccgccc actggaaagg
    20521 aacaacacca ataggacgac cggaatctac accgctgtcg atcacaatgc ggacttcagc
    20581 atgcagaact gatgcccaca gtatgagaaa accaaatgct actcgtaatg cctgcttcat
    20641 catatctccc ttatctggac cgatggccca cgataattta gcagaatgtt aacaaactaa
    20701 aatacacaaa actaccagaa ccccgtggca accggtgcct gatgttgacc gtccgaacag
    20761 tcaacatcgc gattacggtt tgaagtccaa tggcgcgttt ttgaacactt catatactgc
    20821 ctggcttggt ggtttcggga tcttcgcaag tttagctgct gccaacgcag cctgacaaag
    20881 tgcgggatcg ccaccttcag gtttgatatc cagtaacata ccatcgggtg ccagttttat
    20941 gcgcagcgta caggttttgc ctgcatagga cgatgcgtca tagaacttac tttcgatagc
    21001 agatttaatc tgcccggcat agttattgat atcggcccct gatgcgccat tgtttttagt
    21061 attaccactc ccggcaggcg aagcattgtt ccctttcgcc cctccccccg ttttcggtgc
    21121 attcttacca gagcttagct caccgaaaat atcatctgcc tctgcggcag cttttgctgc
    21181 agcggctttt tcagctgctg cttttgccgc tgccgctttt ttgtctgcgg cggctttttc
    21241 tgccgctgct ttcttatcag ctgctgcctt atcagcagcc gctttttctg ctgctttttt
    21301 gtcggctgca gctttctctg ctgccgcttt cttatcagct gcagcctttt cagcagccgc
    21361 tttcttctca gcttctgcct tggctttttc agcagcttca gttgccgctt tctttcttgc
    21421 ttcagctgca gctgcttctg ccgcttccgc tttcttcttc agtgccgcag cggctacctc
    21481 ggcttttttc tgcgcttcgg ctgcggcttt ggcggcttct gcttctgctt ttttcttcgc
    21541 gtctgcagcc gctttcttcg ctgcttcttc cgcagcttta gcatctgccg ccgctttcgc
    21601 tgccgccact tcagcttgct tctgctttaa ctcggcctgt tttgcggctt cttcagcctg
    21661 ctttttctgc tcctgagccg ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc
    21721 agccgcttgt ttctcacgca gttcttcagc agcctgctgt tccttcatct tgcgctgttc
    21781 atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt ttgtactgct caactaccgc
    21841 acctgaatca accatgacag cgtcgatgga cgaaccaccg ccgcctccgg ctgaagcttc
    21901 tatattctca tcgaacgaac tccagatcag cgccgcaaat aagatgacat gcagcactgc
    21961 tgaaataatt atcgcccgct tgagcttgtc gttttgttcg gttgcctttg acactctcgg
    22021 tttccaaaaa ctgttcgcct gttacccgct ctctttcaag caagggaaac gcagatgttt
    22081 agataggctg cgtcattaaa ccaaccgatt tcacacccgc actatgtaac aagttcagtg
    22141 ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc gatcagaaag accgttttcg
    22201 ggttggcctt gaaacggctg gacacttccg ccaccacctg ctctggtggt aaacgctcca
    22261 ggcgatcttt ctcaaccacc acggtgtact gaccaatacc agacacttca acaatcactg
    22321 gcggattatc gttactgctc accgcctgtg attcagtagc gtctggcaga tcgacctcca
    22381 cgctctgggt gatgatgggc gctgtcgcca taaagatcag caacagcacc agcagtacgt
    22441 ccagcaacgg aacaatgttg atttcggact tgagatcgcg acgacctcgt ccacgcgctc
    22501 tggccatggc ttaccccttg ttgctctcgc taacggtaaa cgcctggcgg tgcagaatcg
    22561 cggtaaactc ttccataaag ttgtcgtaat tcagttccag tttgtttacg cgctggttga
    22621 gacggttata agccattacc gccggaattg cggcgaacag accaattgcc gtcgcaatca
    22681 acgcttctgc gataccgggc gcaaccattt gcagtgttgc ttgttttacc gccccgaggg
    22741 cgataaaggc gtgcatgatc ccccagaccg taccaaacag accaatatac gggctgatgg
    22801 agccaaccgt accgaggaac ggaatgtgcg tttccagatt ttcaagttca cggttcatgg
    22861 agatacgcat agcacgcgac gccccttcca ctacggcttc cggcgcatgg ctattggcac
    22921 gatgcaggcg cacaaactct ttgaacccgc tgtaaaagat ttgttccgaa ccagtcagat
    22981 tatcccgttt cccctgactc tcttgataga ggcgagagag ttcgattcca gaccagaatt
    23041 tatcttcaaa cgcttcggct tcacgcgccg ctgcgttaag aatacgggtt cgctggataa
    23101 taatggccca ggatgcgatt gaaaaaccaa tcaaaatcaa catgataagt ttaaccagaa
    23161 ggctagcctt caggaacaaa tcaaggatat tcatgtcagt cactgcttaa actccgcgac
    23221 aatagacttg ggaagcgcac gaggcttcat tttgagtggg tcaacgcaaa caaccagaac
    23281 ctctgcttca ttcagcaaag tattctcggc gttgacaata cgttgcgtga aaaccaaaga
    23341 ggtgccacgc attgatgtta tttcagtctg tatttcgagc atatcgtcga gccgcgcagg
    23401 tgcgtaatat tccaccgtca ttttacgtac cacaaaggca acgcgttcag ccatcagcgc
    23461 ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt gctctttcat aaaaagcgac
    23521 gtaactggcg tggtacacca caccaccggc atcggtatct tcatagtaga cgcgaaccgg
    23581 ccatcgaaac agcgttgtat tcactttaca tcccggtaat gcaacaaaag ttagagcttt
    23641 taaacgctgc tactatacgc gcgcgattga ggtttgggaa tgagggcaag ttaagggcgc
    23701 gtaaaaattt atgggcctag agcggcccat aattttttaa agagaattct gatttaaaag
    23761 aagaagaaaa tcagcccgac aatgagaaca atatcggcaa gcaatgggca aaaaatccct
    23821 tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa tcacaccagc acagacggcc
    23881 cacatcagca ataaaccatg ccagatttcc agttcactgg tcttcgcggc aaaacgagat
    23941 gggtcccaaa acatacatcc tgctaacaga agcgccatca cgaaggaaag cgcccgcagg
    24001 gggcgcttgt ccattaccgc atacaaggtc gcgataatct tactcatcag atgtcttctt
    24061 gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc ggtgattacc ccaaacgaac
    24121 aggcaagaag cgttcccaga atccatgcga aataccacat ttttagctcc ttacttagta
    24181 cagagagtgg gtgttacgtt caatatcttc tttggtgata cgaccgaaca ttttccagta
    24241 acaccaggcg gtgtagagca gaatgatcgg taccagaacc accgcaaccc aggtcatgac
    24301 gttaagcgtc agctggctgg aagttgcatc ccacattgtc agacttgcgt tcatcatggt
    24361 gctggacggc atcacaaacg ggaacattgc gataccggct gtcaggatga tgcaggccag
    24421 cgtcagggag gagaacacaa acgcccacgc ggctttatcc atacgtgcag tcaggatggt
    24481 cagcagcagc agaaccacac ccagtgccgg aatagcccac agaattggcg tgttgttgaa
    24541 gttaaccagc catgcgccag cttcacgaac cacttcttta ttcagtgggt tagaggctgc
    24601 gtaatggtcc attgtcgatt tcacgacata accatcgata ccgtacatca cccatacgcc
    24661 agccagtgcg aaacagacca gtgtcaccag cgcagccacc tgagccgttg cacgggtacg
    24721 caggtgcagt tcgcccacgg tacgcatttg cagataggtt gcgccctgag taatgatcat
    24781 ccctacgctc accacgcctg ccagcaggcc gaacgggtta agcaactgga agaagttacc
    24841 ggtgtagtac agacgcagat attcatcaac gttgaacggt acgccctgca acaggttacc
    24901 gaacgctaca ccaattacca gcggcggaac gaagctacca atgaagatgc cccagtccca
    24961 catgttacgc cagcgggttt cttcaatctt ggagcggtag tcaaaaccga ccggacggaa
    25021 gaacaaagac gccagcacga ggatcatcgc cacatagaag ccggagaacg cagcggcata
    25081 gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg atcagccaaa cctggttacc
    25141 gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc tcggtgtcgt tacgaccgag
    25201 gaaacgggtg agcatgccca cccccatgtc gaaaccgtca gtgactgcaa aaccaatcag
    25261 cagaacgcca accagcagcc accagataaa acgcaatact tcataatcga tcatttgacg
    25321 actcctgtct tagcgtgccg gctgagtagt cgtggaagac tgctcaaagt gatagcgacc
    25381 ggttttcagg ctgcttgggc cgaggcgtgc aaacttgaac attaagaaca attctgccac
    25441 caggaacagg gtatacaggc cgcaaatcag caccattgag aagatgagat cgcctgcggt
    25501 cagtgacgaa ttcgccacag ctgtcggcag cacttcaccg atagcccacg gttggcggcc
    25561 atattcagcc acgaaccagc ccgcttctac agcaatccac ggcagcggaa taccgtacag
    25621 cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg cggatgacac tccagaaaga
    25681 gagcgcgatg attgccagca gcaggaagcc acacgccacc atgatacgga acgcaaagta
    25741 cagcggcgct acacgcggga tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc
    25801 atcagccacg tttggcgtat agcgtttcag cagcagaccg taaccgaggt ctttcttcat
    25861 gctattgaac tggtcacgaa ccgtctggtc ggtagaacca gaacgcagtt gttcgagcag
    25921 agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc tgcaccatca gctctttcag
    25981 gccgataacc ggggtatcca cggaacgcgt tgcaatgatg cccagtgcgt aagggatctg
    26041 aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg ccgaacaaag taaaggcagc
    26101 aggcgcaggt tgcgtttccc actcggcttc aatagcagcc agtttggttt tctgcacgtc
    26161 gcccatttcg tagccggatt catcacccag aacaataaca gacagaacag cagccatacc
    26221 gaagctggca gcgatagcaa aggagcgttt agcgaaggcg aagtcacgac ctttcagcat
    26281 ataccatgcg ctgataccga ggatgaacat cgcgccagtc acataaccag acgctacagt
    26341 gtgaacgaat ttcacctgag caaccgggtt aagcaccagc tcggagaagc tcaccatctc
    26401 catacgcata gtttcaaagt tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc
    26461 aaccagaatc cacagtgcgg acaggtttga accgagcgcc accagccagg tgacacacat
    26521 atgctgaact ttacccagac gatcccaacc gaagaagaac agacctacaa aggtggattc
    26581 gaggaagaag gccatcagac cttcgattgc cagcggcgca ccgaagatat cccctacata
    26641 gtgggaatag taagaccagt tagtcccgaa ctggaactcc atggtcagac cggtagccac
    26701 acccagagcg aagttgatac caaacaactt gccccagaac ttggtcatat ctttataaat
    26761 ctgtttgccg gagaggacgt agaccgtttc cataatggcc aggaggaacg ccataccgag
    26821 cgtcagtggc acaaaaagga agtggtacat cgcggtcaag gcaaactgta agcgcgacag
    26881 ttcgactata tctaacatca tgactccttg ctcatcgcat gaagactccg agagtgaacc
    26941 ccgttagaaa gggtcacacg catgccccaa tagaaatttg tttgaacact ttcatcatac
    27001 catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca aacatgttaa taaaaactca
    27061 aattgatccc acgtatatat tacgccgcaa aatccttaca ataaacaggt ttttgttgaa
    27121 gcaaatttgc atttttcgac ggtgatcaat ttatagctaa attgccgcct ttcagccaat
    27181 ttgatcgaga acaatttatc tctttttgat gcccatttcc aagacttata cattgataaa
    27241 tatcaatttc tacaaattat gttaatcatg tttcaacaag gtaactttag tgatatttat
    27301 gtttaaaatg tgtgaattat aagactgaaa ttagtttcgg ttggcaatta aagataaaaa
    27361 gagacgaagc aaaaacagca cggttgacct taattctttt tttggtcact ggctaatacg
    27421 acgcagagtt tcaggattat taataaaggc ataaatctcc ctcccgccga tattaacatt
    27481 taattaactt ataattatat tataattaac tcacccgtgg ttattatttt gttaaaaata
    27541 aagccacata tacgtagctt gagagtgaag ataaacacaa aattacctga tgcgctacgc
    27601 ttgccagtgt ctacatcatc tctgcaatat attgaatttg cctgcttttg tagaccgaat
    27661 aaagcgttca tgccgtatcc ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc
    27721 atatgtggct tcggtttgat tgctattcag gcaagccggt aactgaacgt ccgtgactgg
    27781 ccgggtaaaa aaggccccga tagatttgaa ccttgatttt cctcggtggt aatgtgttca
    27841 tccatcatcg tttctgagca agaaatcacc tcgcgactga aagcaacagt cgcatcacag
    27901 gttgctgatt cagccggatt aaacagccgt aaaatcactt cttgtcggtc ttcagctttc
    27961 ttaagtgcgc ttatcaggca tcccactggg ggcattttca acaaactata actttccggc
    28021 acgttgaatc cggctttgtt gagcttcatc gcatcccatg ggattttgtt gtaacactgt
    28081 actggagtca gccatgctcg cgcctgctga gctacaccag cggcggttgg cgtaccggta
    28141 taactcaata aacttaggcg acaagaaagc agaccacgta gttgtgagtc cgggactggc
    28201 attttaattc ccgaaggccg cctaggcctt aaaagcagat cttctttccc cagtaagccc
    28261 acgccgcgca gcaacgtaat ggcaaaggtt ttcttctctt caccgatgac ttcaaattca
    28321 cgtagccctt cgctaaagac agccatgccg ttacgccctt cctgtaaggc tgcatagttg
    28381 agcatattcc ataccggaac cggcgcttct ttccagcttt cttgctgcca gttgttcatt
    28441 gcactgtcgt tcacggggcg cgttagcgaa ccaaactggg tatctgccag aacactatcg
    28501 gtgttaaaag atgtagggat caggacgcga aggcgatgat cgtcagcctg gttatcaagg
    28561 ttgatatcca catcaatgcg cctgttgtta tgactaagag tgacaaccaa ctcgacatct
    28621 actctgccag tggattgtct tgcgctgcgt tgttgcagat tacgcggcac cgccatttca
    28681 tagcggataa cagccctgct ctgccaggcc tcatgaataa tctcgcattg cggtttcgca
    28741 ttcgccgaag taattaccca ctcttctttt gcgggtgaat agtcatattc atcaccatca
    28801 tcagagcttt cttcaatttg caacacccgg tcatagcgca caccgctatc tttatctacc
    28861 agttgtagcg ttccatcctc attgagcgca atttgccaga aagcattttc cagtatccct
    28921 tcagcgtcac tttttgccgc aatcacgttg ccaggctgat tcgcttcgat ataaagcgtg
    28981 cgatagccca tagaagggac aatctggttg atctgtatat caaactccat aaagggatcg
    29041 taattaccgt aatgaactat ttgccgatcg attaggcctg gatcgatctc acgggcatgg
    29101 cgaataaaat acggtacagg ctgaccgcga tcgtctcgta gattaaactg gctggcgcgc
    29161 agccgcacag tggtgttgat cacttcttcc cgcggccagg gcatcaggtt aaacaggacg
    29221 agtttgtcgg cgtcgctctg cggcatgttg tcggcgattt tacgcatata gaaacgcagc
    29281 agattatccg ccatgtcttc agccagttcg aagcgggcga cgatttcgcg atgaactttg
    29341 tcacgacagc agcagccgat gctgtcgtgg gcatgatttt ttaagatctc tttccacatt
    29401 ttctccagca agccgtggtg gtatccaaaa cccaacgtcc tggccagcgt tgccagcggt
    29461 tccagcagat taacaatctt attttcaata cgtgcgtggg caactttgat atccatgcgc
    29521 gtggaaccga tggtgcgatg cacgcgcata tatttgccat caataaattc ccctttcagg
    29581 gttgccagat tatctcgctg cgcttcgatc ttctcaaata cctcttcaaa gcggctcatc
    29641 acaaactttc gttgaggata aatttcgcgc agtttctcca tcacttcgaa gatattttgc
    29701 tgcaatggca tctgatcatg cccattcggc agcaaaatct ctttggttac agacgctttt
    29761 tccagcacgt caaaataact gtcgaggcgt ttgcgtaatc cgttttcgtc cgcaggtaag
    29821 tacttaccga tggcataacc caacggcatc acctgcgccg tcacttcgct accgtcactg
    29881 ctttgccaca aaaactcggt tttatccgta ccgtggcgct cagaacatcc acgccagaac
    29941 atggtgcagg taatgccaaa tccattgtag atatgcggca gttgcccgga catgccaaag
    30001 gaatcgggta aataacctat tttcatcggc tcgccaaacg cgaggcagtc acgcattccg
    30061 tacatcagat tacggacgat ggattccgca gaaacaatcg tggtatcggt ctgggtatac
    30121 caggggccga taatcaactt gccggcttct accagtttct tcaagcggtc tttgttttcc
    30181 ggtttcaccg cgaaataatc ttcgaggatc gccgtttgcc cgtcgagtac gtaatatttg
    30241 tattcgttgt cctgttccag tcggcacagg atctcttcca tattattgac cagcagaatg
    30301 cgtgactctt cggtggtgaa ataccactcc cgatcccagt gcatatgcgg ggtgatgtga
    30361 acgcgagata ccgctttcat cgtcgtttcc tttttgttta tggcattacg ccatcagtca
    30421 gatagttgcc atgcttaacc gcgtgacgcc gccagatcag gagaattgca gtcgagattg
    30481 cagcccccac caatgccgca ccaaaccagc caattgccgc cataacaccg cccgcgccat
    30541 tatcatgaag taaaaagagc gagaaaatgc cagcaccggg tgtcgaaagg ccgatattca
    30601 tcgcaccgac aatagcgccc gttaccatag agcccagcac aaacgaacca ataacccgca
    30661 gcggatcttc aatcgccatc gggatcgccc cttcggtaat acctgccagc cctaacagcc
    30721 aggtggattt cccggtctca atttcaaact ctttaaacag gcgcggtgcg agcatcgtgg
    30781 aagcggttac ggtaaatgcc gaaaccattt tgacggaggc gaaaatggca tacgggccgt
    30841 aaacgccgtt cgccattgcg cccaggcaga atgcataagc ggctttattc actggccctc
    30901 caaggtcaaa ggaacacata aaaccgagaa tggcacccag caacagcgcg ttacttcctg
    30961 acagaccgtt cagccaggcg gtaagcgagt tattgatcca ggcgacaggt tcccccacca
    31021 caaacagcat cagactgccc gctcccaatg taccgagcac cgggtagaga taaaaagtca
    31081 ggaagccatt gaatttactg ctaagacgca agtgattttt catccagcgt atcaagtaac
    31141 cggctatcaa tccgccaacg accgcgccga gaaacccgga gccgatcatg ttggcggcaa
    31201 gtccagccgc aaagcctggc gctaacgccg gtttatctgc cagcgaatag gcggtatagg
    31261 ctgccagcac cggtaccatc aaaattccga gcagcccgcc gcccagtttg cggtacatcc
    31321 acagccagga gttttcttca ttaaacagat cttgtagccc gaagatttgc gacagtaata
    31381 ccgcgaccgc cagcaccgtc ccccccgcga caatcaacgg tacggcaaaa gagatcccgc
    31441 tcaacagtgc ctgtttcagc tccgttttga cgcttttcac cggttgcgta tcttgctgta
    31501 aggtacgcgt ctcatcgcca cgctcgaggg taagcgattg ttggatcaac gcttctgcat
    31561 ggcgaatcgg ctcggcaaca ggcacagaaa gcgcaggaat gccgttaaaa cgctcactct
    31621 ccttgatggc gacttcagcc gcaaaaatac aggcagttgc actattgagt tgatccgccg
    31681 ttaaacgccc ttcaataccg ttagcacctt gcttttcaac gtagacattc acgccgagtt
    31741 tgcgtccggc tttttccaga tactccgcag ccatgtaggt gtgagcaata cccgccgggc
    31801 aggccgttac gcaaacgata gttggcgcgt tggaaaaaga ggcagaaggt tgcgtgcctc
    31861 ccttgtcatc cagcgccgag agcaattcat caggcgtcgt cgccgactgt atacgcgccc
    31921 gaatctcatc atccgccagg cgcgtggtca gcgctgtcag cagttgcatg tgcgtagtac
    31981 ccgcttcatt gaggggaatc gccagcaaca ccactaaatc aaccgcttcc gggccatcca
    32041 cgccttccca ctgaagcggc tcgctgagtg tggcgaccgc aaacgccgct tcttttaccg
    32101 cagcagtttt gccatgcggc acagccaacc cttcacttaa ggcggtcggg ccaaggcttt
    32161 cacggcgata cacttctttc agaaattgct cagtactgga aattttcccc agagcagcaa
    32221 gacgttgagt caacgcgtga atggcctctt cacggctggt aaagcgcgca ttcagacaca
    32281 acgcatcgcg gtgggtcaga gtcgtcaggt tcatcatgat cctcacttga tcgtttttat
    32341 agtcgcgcca gtgtgcccga caaaacaata ccattgcgaa atcaatttca cttgattgaa
    32401 attatttgca ttttatttgt attgtttaca ggatgagttt atcgggcata atggctaaat
    32461 tcgtattaat gagatgctaa ataatgggac acaaaccctt ataccgacag attgctgatc
    32521 gcattcgtga gcaaattgcg cgtggcgagc ttaaacctgg cgatgcgtta cctaccgaat
    32581 cggcattaca aacagagttt ggcgtcagcc gggttacggt gcgtcaggct ttgcgccagt
    32641 tagtcgagca acagatcctc gaaagcattc agggcagcgg gacttacgtc aaagaagagc
    32701 gggtcaatta cgatattttt cagttaacca gttttgatga aaaactgtcg gaccgtcacg
    32761 tcgatacaca cagtgaagtt ctgatatttg aagtgattcc ggctgacgat tttcttcagc
    32821 aacagctaca aatcaccgtg caggatcgtg tttggcatgt gaagcgcgtg cgctatcgca
    32881 agcaaaagcc aatggcgctg gaagaaacct ggatgccgct tgcgttgttc ccggatctca
    32941 cctggcaggt catggaaaat tcgaaatatc actttattga agaagtgaag aagatggtta
    33001 tcgatcgtag cgaacaggaa atcattcctc tgatgccaac cgaagagatg agccgtctgc
    33061 ttaatatcag ccagacaaag ccgattcttg aaaaagtatc gcgtggatat ttagttgatg
    33121 gtcgggtgtt tgaatatagc cgcaacgctt ttaataccga tgactataaa tttaccctga
    33181 tagcgcagcg aaaatcatcg cgataagcac aaaaaggcca tcatacgatg gccaaccatg
    33241 tcgaaaccga cagcccttcg ccattcctgt agagggaagc ggcgagggct atttcttatt
    33301 acagatattt atttcagaac agttttcagt gcttcaccga tatccgccag gctgcgaacg
    33361 gttttcacgc ctgcggcttc cagagcagcg aatttctcat ccgcagtccc tttcccaccg
    33421 gcaatgatcg cacccgcgtg gcccatacgt ttgcctttcg gcgcagtcac accagcgatg
    33481 taacccacaa ctggcttggt aacatgctct ttgatgtacg ctgccgcttc ttcttcagcg
    33541 ctaccgccga tctcaccgat catcacgatc gcttcggtct gcggatcttt ttcgaacatt
    33601 tcgagaatgt cgataaagtt agagccaggg atcgggtcac cgccgatacc gacacaggtc
    33661 gactgaccga aaccgtaatc cgtggtctgt ttaaccgctt cataggtcag tgtaccggaa
    33721 cgggaaacga tacccacttt acccggtttg tgaatgtgac ccggctggat accaattttg
    33781 cattcacccg gggtgataac gcctgggcag ttcgggccga tcatacgaac gcctgcttcg
    33841 tccagtttca ctttcacggt cagcatatcc agcgtcggga tgccttcagt gatggtgata
    33901 atcagtttga tgcctgcatc gatagcttcc agaatggagt ctttgcagaa cggtgctggt
    33961 acgtaaataa cagacgcgga agcgccagta gcagcaacag cttcacgcac ggtgttgaac
    34021 accggcaggc cgaggtgggt agtgccgcct ttacctggag ttacgccacc aaccatttta
    34081 gtgccgtatg caatggcctg ttctgagtgg aaagtcccct ggctaccggt aaagccctgg
    34141 cagataacct tggtgttttt atcaattaaa atggacatta tttcccctcc actgcggcaa
    34201 caacctgctg agctgcatcc gtcagacctt ttgctgcaat aatattcagg ccgctgtcag
    34261 ccagtttctt cgcgccgagt tcggcgttgt taccttccag acgtaccacg accggtacgt
    34321 taacacccac ttccgctacc gcgccgatga taccgtcagc gatcaggtcg caacgaacga
    34381 taccgccgaa gatgttaacc agaacggctt tcactttgtc gtcagagagg atgattttga
    34441 acgcttcggt tacacgttct ttggttgcgc cgccgccaac gtccaggaag ttagccggtt
    34501 cgccgccgtg cagtttaact atgtccatcg tacccatcgc cagacctgcg ccgttaacca
    34561 tacaaccgat gttaccgtcc agcgcaacgt agttcagttc ccactgtgca gcctgtgctt
    34621 cacgcggatc ttcctgcgac tggtcacgca tttcgcgcag atcaggctgg cggaacaggg
    34681 cgttgccatc agcgcccagt ttgccgtcga ggcaaatcag atcgccctgt ttggtgatga
    34741 ccagcgggtt gatttcgatc agcgccaggt cgcgctccag gaaaatggtc gccaggccca
    34801 tgaagatttt ggtgaactgc tgaaccagtt taccttccag acccagtttg aacgccagct
    34861 cgcgtccctg atacggcatc gggccagtca gcggatcaag cgcaacttta tggatcaggt
    34921 gcggagtttc ttccgccact ttttcgattt ccacgccgcc ttcggtggag gccataaaga
    34981 ccacgcgacg ggaactacgg tctacaacgg caccgagata cagctcttta gcgatatcgg
    35041 tcgccgcttc aaccagaatc tggttaaccg gttggccatt ggcatctgtt tgatacgtta
    35101 ccagacgctt gcccagccag ttttctgcaa aagcacggat gtcttctttg ctgtttacaa
    35161 ctttcacacc gcccgcttta ccgcggccac cagcgtgaac ctgacatttc actacccacg
    35221 gaccggcacc gatttttgaa gcggcttctt ctgcttcgcg cggagtagta caggcataac
    35281 ccaccggtgc tggtaagcca tagcgggcaa acagttgttt tgcctgatat tcatgtaagt
    35341 tcatgtgttc tgtccatcct tcagtaatcg ttatctttta aaccgtagac cgacacccta
    35401 tccacgtcgg tctacagtgc aggtgaagct taaactacta cacgtccagc agcagacgcg
    35461 tcggctcttc cagtaactct ttgatcgtta ccaggaagcc cacggattcg cgaccatcga
    35521 tcagacggtg atcgtaggac agcgccaggt acatcatcgg caggatctca acctgaccat
    35581 tcaccgccat cggacgatct ttgatagcgt gcatacccag aattgcgctc tgcggcgggt
    35641 tgatgatcgg cgtagacatc agggaaccga acacaccacc gttggtgatg gtgaagttac
    35701 caccggtcag atcttcaacc gtcagcttgc catcacggcc tttgactgcc agctctttga
    35761 ttttcttctc gatgtctgcc atgccgaggg tatcgacatc acgcagaacc ggcgtcacca
    35821 ggccgcgcgg cgtagaaacc gccatgctga cgtcgaaata gttgtggtaa accacgtcat
    35881 cgccgtcgat agacgcgttc acttccgggt aacgtttcag ggcttcaacc accgctttca
    35941 cgtagaagga cataaagccc agacggatgc cgtggcgttt ttcaaacgct tcaccgtact
    36001 gcttacgcag atccataatc ggcttcatgt tgacttcgtt gaacgtggtc agcatggcgg
    36061 tggagttttt cgcttccagc agacgctctg ccacacgctt acgcaggcga gtcatcggga
    36121 cacgtttttc actacgtgca gccagagccg gttgcgccgc cggagcagcc gctgccggag
    36181 cagactcttt cgccggggct ttcgccagat gtttttccac atcttcacga gtcagacgac
    36241 cacccacacc ggtgccttta atggcgctgg cgtcgagatt gtgttcagcc agcaggcgac
    36301 ggatcgccgg gcttaacgca tcgttgtttt gctcttccag agacgcctgc tggcgttgcg
    36361 ccggagtgga cgctttctct tcagatttgg cgctggtttc tttaccggcg ctgttgcctt
    36421 cacgcaggcg accaaggatc tgacgagacg ttaccgttgt accttcatct tccagaaccg
    36481 catccagaat gccgtctgct gatgccggta cttccagtac cactttgtca gtttcgattt
    36541 ctaccagcac ttcatcacgt acgactgcgt cgccgggttt tttatgccag gttgcgacgg
    36601 tggcatcggc tacggattca ggcaggtcag ggaccagaat atctacgcta ctcattgtgt
    36661 atcctttatt tattcgacgt tcagcgcgtc attaaccaga tcttgttgct gtttctggtg
    36721 aacggacata taccctaccg ccggagaggc ggaggccggg cggcctgcat aacgcagaga
    36781 agccccaaac ggaatcactt cacggaaatg atgctggctg cagtaccatg cgccctggtt
    36841 gagcggctct tcctggcacc agacaaaatc cttgacgtga gcaaactgct gcaacacttc
    36901 ctgcatcgct ttatgcggga acgggtagag ttgctcgata cgcacaatgg cgacatcgtg
    36961 ttgattgttc ttacgacgct gttccagcag gtcgtaataa accttaccag aacacatcac
    37021 tacgcgcttc acgcccttcg gatcaagctc gtcgatttca ccgatggctg gcaggaaggt
    37081 gccgttcgcc agttcttcga ggctggaaac cgccagcgga tgacgcagca gggatttcgg
    37141 cgacatcacg accagcggac gacgcatccc gcgcagcgcc tgacgacgca gcatgtggta
    37201 aacctgtgcc ggggtagacg gtacgcaaac ctgcatgttt tgctcagcac aaagttgcag
    37261 ataacgttcc agacgcgcgg aggagtgctc cggcccctgc ccttcgtaac cgtgcggcag
    37321 caacatcacc agaccacaca tccggcccca tttctgctcg ccagaggaga tgaactggtc
    37381 gataaccacc tgcgcaccgt tggcgaagtc accgaactgc gcttcccaga tggtcagggt
    37441 gcgtggttct gcggtggcat aaccatattc aaacgccagc acggcttctt ctgacagtac
    37501 ggagtcccag acacggaacg cgccctgacc gttatggatg tgttgcagcg gcgtgtaagt
    37561 ggaaccgtta gactggttgt ggatcaccgc gtggcggtgg aagaaggtac cgcgaccgga
    37621 gtcttcaccc gacaggcgaa ccggaatgcc ttcatcaacc agcgtggcgt aagcgaggtt
    37681 ttccgcaccg ccccagtcga acagtttctc accggctgcc atcgcctggc gatcgccata
    37741 aatcttggca acgcgagact gcatttcaac tgcttctggt accgtactga tacgtttagc
    37801 cagttcctgc aggcgcttca tctcaacttt gttcgggtac tcttcgtccc attcgtggtt
    37861 gaggtacggc gaccaggtga aagagtgcat gttcatcgga cgccactctg ccactacgca
    37921 atcgccagca tccagcgcat cgcggtacag gttaaccatc tcggtggcat cttccagcgt
    37981 cgccactttt tcctgctcca gcttgtcagc gtagattttg cgcggtgtcg gatgtttttt
    38041 gattttctga tacatcagtg gctgggttgc gctcggctcg tcggcttcgt tgtggccgtg
    38101 acggcggtag cataccaggt cgatgaagac atcacgttta aaggtgttac ggaaatcgag
    38161 cgccagacgg gtcacaaagg caacggcttc cggatcatcc gcgttaacgt ggaaaatcgg
    38221 tgcctgaacc atcttaccga tatcagtaca gtacggtgta gaacgggcat ccagcgggtt
    38281 agaggtggtg aaaccaacct ggttgttgat aacgatacgt accgtaccgc caacttcata
    38341 accacgcgct ttcgacatgt tcagagtttc ctgaaccacg ccctgcccgg tcactgcggc
    38401 gtcaccgtgg atggtgattg gcagcacttt gttgctgttc ggctcatcaa gtctgtccag
    38461 acgggcacga acagagccga taacgaccgg gcttacaatc tcaaggtgag acgggttaaa
    38521 cgccagcgcc aggtgcacca ggccgccatc ggtctggaag tcagacgaga agcccatgtg
    38581 gtatttcacg tcgcctgtgc cgaggtgttc tttatgttta ccggcgaact cgtcgaacaa
    38641 gtcttgcggt tttttaccca gcacgttcac cagcacgttc agacgaccac ggtgcgccat
    38701 cccgagaacc acttcgcggg tgccgctgtt gccagcgtgg cggatcatct ctttaagcat
    38761 cgggattaac gcgtcaccgc cttccagcga gaagcgtttt gcgccaggga attttgcgcc
    38821 gaggtaacgc tcaaggcctt cagcggcggt cagttcgctt aagaagcgtt ttttctcttc
    38881 gctattgaaa gtcgcgcgac cagactcgat acgctgttgg atccagcgtt tttcttcggt
    38941 gctggtgatg tgcatatact cggcaccaat cgggccgcag taggtttgct tgagggcgga
    39001 aataagctcg ccgagtttca tggtttcttt gccgctggca aatgaaccga cgttgaaggt
    39061 ttcctggaag tctgcttcgg tcagatcgtg gaaagacgga tccagatcgg ccactttatc
    39121 ttgctgccac agtcccagcg gatctagatt cgcatgctgg tgaccacgga agcggtatgc
    39181 gttaatgagc tgcaggactt taacctgctt cacattggtg tcagggtcgg agatcgttga
    39241 agagtaacgt gaagcgtctt tcgccaggcg gcggaaatat tcacgcgttt gagagtggaa
    39301 ttgatccggt ttgactcccg taccaggtaa ctgctggaac gtcgaacgcc agttagcgtc
    39361 aaccgagtca ggatcggtta agaagtcttc atagagctgt tctatccagc tctggtttgc
    39421 gccagagagg taagaagagt ccaaccaggc tttcaaagcg ctgttctgca tcgtgatccc
    39481 ttaagcatct tttttatgct tacttcgccg tggatactac cacgcacagt gatgtgcgtg
    39541 ccggggttca cttgcgagct cttgtctacg tagtggctcg cgaaggaacc tttaaaaact
    39601 gtcttagtgt caggggcagt ttttaaagat ttcctgcatt tctttgttac gcctgatgcg
    39661 ctgcgcttat caggcctgcg ggtaatgact tgtaggccgg ataaggcgtt cacgccgcat
    39721 ccggcactgg ttgcctgatg caacgcttgc gcgtcttatc aggcctacag tttacgcatt
    39781 acgttgcaac aacatcgact tgatatggcc gatggcgcgc gtcgggttca gccccttcgg
    39841 acatacactg acgcagttca tgatgctgtg acagcggaat acgctgaatg catcgctcaa
    39901 accgtcgagg cggctgtcag tctcggtatc acggctatcg atcaggaaac gatatgccgc
    39961 taacaagcct gccgggccga taaacttatc gggattccac cagaaagacg gacaagaggt
    40021 tgaacaacat gcgcagagaa tacattcata cagcccgtcg agtttttcgc gctgctctgg
    40081 catctgtaaa tgctcgcgag ctggcggatt ttgtccatta ttcaacaggt aaggcttaat
    40141 tttctcatat tgcgcataga attgtcccat gtctaccacc aaatcgcgga tcaccggtaa
    40201 acctggcagc gggcgaatca caatcttctt gcccggctgg ttgagtgccg aaatcggggt
    40261 aatacaggcc agaccattct tgccgttcat gttcagaccg tcggaaccgc acacaccttc
    40321 acggcaggag cggcggaacg acaggctggg atctttctct ttcagctgga taagcgcatc
    40381 cagcagcatc atgtcgcgac cttcttccgc ttccagggtg taatcctgca tacgcggagc
    40441 atcatcaaca tccgggttat agcgataaat tgaaaactcg agtctcattt tcctgtctcc
    40501 gcattagtaa gtacgaatct tcggcgggaa tgccgggcgc agtttcggtt ccatgttgac
    40561 gcttcggcgc gtcatggatt ccgactcagg cagatacagg gagtggcaca gccagttttc
    40621 atcatcacga tccgggaagt cgaagcggct atgtgcgcca cggctttcgg tacggaagtt
    40681 ggcagaaaca gccgttgcat acgccgtttc catcaggtta tccagttcca ggcactcaac
    40741 gcgctgggtg ttgaactcgc tggaagtgtc atccagacgg gcatttttca gacgctcgcg
    40801 gatcactttc aactgctcaa gccctttcgc catcgcatca ccttcacgga agaccgagaa
    40861 gttatgctgc atacattctt gcagcgcttt acggatcgcc accggatctt caccgttacg
    40921 attattgttc caccggttca ggcgatccag agacgcttca acatcagact cgctggcatc
    40981 gtgcagtgcg ccctgctcgg cgatagactc ttgcagatgc agacctgccg cgcgaccaaa
    41041 gaccaccagg tccagcagcg agttgccgcc cagacggtta gcgccgtgta ccgatacaca
    41101 agcgatttca ccaacggcaa acagtcccgg aacaaccaca tcttcgcctt tctcattcac
    41161 agtcagcgcc tgaccggtaa ctttggtcgg aataccgccc atcatgtagt gacaggttgg
    41221 gataaccgga atcggctctt tcaccggatc gacgtgagcg aaggtacggg aaagctccag
    41281 gatacccggc agacgggatt cgagaacttc tttacccagg tgatcgagtt tcagtttcgc
    41341 gtgtggcccc cacggaccat cacagccgcg accttcacgg atttcaatca tgatggaacg
    41401 cgccaccaca tcacggcccg ccaggtcttt ggcgttcggc gcataacgct ccataaagcg
    41461 ttcgccatgt ttgttcagca gataaccgcc ttcaccacgg caaccttcgg tgaccagtac
    41521 gcccgcaccg gcaatgccgg tcgggtggaa ttgccacatt tccatatcct gcaccggtac
    41581 gcctgcgcgg atagccatgc cgacaccgtc gccagtgtta atgtgggcgt tagtggtgga
    41641 ctgataaata cgccctgctc cgccagtcgc cagcacggta gcgcgggctt tgaaataaac
    41701 cacttcaccg gtttcgatgc acagtgcggt acaacccacc accgcgccat cctggttttt
    41761 caccagatcc agcgcatacc actcggagaa aatggtggtg tggtttttca ggttctgctg
    41821 ataaagcgtg tgcaacagtg cgtgaccggt acggtcagca gccgccgcag tgcgtgccgc
    41881 ctgctcgccg ccgaagtttt tcgactgacc gccaaacgga cgttgataga tacgaccatc
    41941 atcgagacgc gagaacggca ggcccatgtg ttcgagttcc agaatcgctt ccggcccggt
    42001 tttacacata tattcaatcg cgtcctggtc accgatatag tccgaccctt ttacggtgtc
    42061 gtacatatgc cattcccagt tatcttcatg ggtattaccc agcgcaacgg taatgccgcc
    42121 ttgcgcagaa acggtatggg aacgggtcgg gaagacttta gagagcaggg cacaggtctg
    42181 gccgctctgg gaaatttgca gcgccgcgcg catacctgcg ccaccggcac caatcacaac
    42241 tgcatcaaat tctctgactg gcaatttcat cacacacccc acaccacaac gaatccataa
    42301 atcacgtaaa ccaccagtgc aacgacaatc accagttgca gcatcaggcg caaggccagc
    42361 ggtttaacgt agtcggtcaa cacctgccac atgccgatcc aggcatgaat caagatagaa
    42421 aacagcgcca gcagggtgaa cactttggtg aacgcagagg cgaagaaacc gatccagact
    42481 tcatatgtca gttcgccact ggtggcgaaa aaaccgacca tataaatgat gtagagcgtc
    42541 aggacgatag cggtagcgcg aacgaggatg aaatcatgta cgccattgcg tcctaatgcg
    42601 gaggcgttgc ttaccatacg aggactcctg cgagaagtga aagcacgaca gtaataacaa
    42661 aggagatttt ggcggagcgt ttacccgctt cgaatgtttc ttccagatag ccaaaatcca
    42721 tcatcatgtg gcgaatacct acgacgacgt gatacgccag agcggtaagg atgccccaca
    42781 tgataaattt gacgaagaag ctgcccataa tcgcggaagc ttgctcgaaa ccttcagggg
    42841 aagagaggct ggtacccaga agccactgca ggatgcccac tgcaacaaag gtgatcacac
    42901 cggaaacgcg atggagaatg gacgctatcg ccgtgatggg gaaccggatg gtctgtaggt
    42961 ccagattaac aggtctttgt tttttcacat ttcttatcat gaataacgcc cacatgctgt
    43021 tcttattatt ccctggggac tacgggcaca gaggttaact ttctgttacc tggagacgtc
    43081 gggatttcct tcctccggtc tgcttgcggg tcagacagcg tcctttccat aactgcgcgt
    43141 catgcaaaac actgcttcca gatgcgaaaa cgacacgtta caacgctggg tggctcggga
    43201 ttgcagggtg ttccggagac ctggcggcag tataggctgt tcacaaaatc attacaatta
    43261 acctacatat agtttgtcgg gttttatcct gaacagtgat ccaggtcacg ataacaacat
    43321 ttatttaatt tttaatcatc taatttgaca atcattcaac aaagttgtta caaacattac
    43381 caggaaaagc atataatgcg taaaagttat gaagtcggta tttcacctaa gattaactta
    43441 tgtaacagtg tggaagtatt gaccaattca ttcgggacag ttattagtgg tagacaagtt
    43501 taataattcg gattgctaag tacttgattc gccatttatt cgtcatcaat ggattcttta
    43561 cctgcaagcg cccagagctc tgtacccagg ttttcccctc tttcacagag cggcgagcca
    43621 aataacaaac gggtaaagcc aggttgatgt gcgaaggcaa atttaagttc cggcagtctt
    43681 acgcaataag gcgctaagga gaccttaaat ggctgataca aaagcaaaac tcaacctcaa
    43741 cggggacaca gctgttgaac tggatgtgct gaaaggcacg ctgggtcaag atgttattga
    43801 tatccgtact ctcggttcaa aaggtgtgtt cacctttgac ccaggcttca cttcaaccgc
    43861 atcctgcgaa tctaaaatta cttttattga tggtgatgaa ggtattttgc tgcaccgcgg
    43921 tttcccgatc gatcagctgg cgaccgattc taactaccta gaagtttgtt acatcctgct
    43981 gaatggtgaa aaaccgactc aggaacagta tgacgaattt aaaactacgg tgacccgtca
    44041 taccatgatc cacgagcaga ttacccgtct gttccacgct ttccgtcgcg actcacatcc
    44101 aatggcagtc atgtgtggta ttaccggcgc gctggcggcg ttctatcacg actcgctgga
    44161 tgttaacaat cctcgtcatc gtgaaattgc cgcgttccgc ctgctgtcga aaatgccgac
    44221 catggccgcg atgtgttaca agtattccat tggtcagcca tttgtttatc cgcgcaacga
    44281 tctctcctat gccggtaact tcctgaatat gatgttctcc acgccgtgcg aaccgtatga
    44341 agttaatccg attctggaac gtgctatgga ccgtattctg atcctgcacg ctgaccatga
    44401 acagaacgcc tctacctcca ccgtgcgtac cgctggctct tcgggggcga acccgtttgc
    44461 ctgtatcgca gcaggtattg cttcactgtg gggacctgcg cacggtggtg ctaacgaagc
    44521 ggcgctgaaa atgctggaag aaattagctc cgttaaacac attccggaat ttgttcgtcg
    44581 tgcgaaagat aaaaatgatt ctttccgcct gatgggcttc ggtcaccgcg tgtacaaaaa
    44641 ttacgacccg cgcgccaccg taatgcgtga aacctgccat gaagttctga aagagctggg
    44701 caccaaagat gacctgctgg aagtggctat ggagctggaa aacatcgcgc tgaacgaccc
    44761 gtactttatc gagaagaaac tgtacccgaa cgtcgatttc tactctggta tcatcctgaa
    44821 agcgatgggt attccgtctt ccatgttcac cgtcattttc gcaatggcac gtaccgttgg
    44881 ctggatcgcc cactggagcg aaatgcacag ttacggtatg aagattgccc gtccgcgtca
    44941 gctgtataca ggatatgaaa aacgcgactt taaaagcgat atcaagcgtt aatggttgat
    45001 tgctaagttg taaatatttt aacccgccgt tcaaatggcg ggttaatttt tatatgccta
    45061 aacacaaaaa attgtaaaaa taaaatccac tatcggacat tcatatattc taaaaaaaaa
    45121 tacatcggta taaattataa acaaatagat gatttttaat catctatctt cctctcagtt
    45181 acatgaataa accaccaaat atagcgcacc cctataattc ccaatacttt attcatcaaa
    45241 catttaattc ataccatcaa ttaattactt ttacatttaa taaaattaat aatattttta
    45301 agaaaaacta aaaacattcg acaatcaaat aagatattct tgcaccgata tacttttttc
    45361 ttttctgtta aaggactcag ttatgtttaa aggacaaaaa acattggccg cactggccgt
    45421 atctctgctg ttcacagcac ctgtttatgc ggctgatgaa ggatccggtg aaattcactt
    45481 taaaggtgaa gttattgaag caccgtgtga aatacatcag gatgatattg ataaagaggt
    45541 tgaactcggt caggtgacca ccagccacat taatcagtca catcacagcg atgccgttgc
    45601 tgtcgacctg ctcttagtca actgtgatct ggaaaactcc agcaacggtt ccggtggcaa
    45661 gatttctaaa gttgcagtca cctttgatag ctcagcgaaa accaccggcg cagatccgat
    45721 tctcaacaac accagcacgg gtgaagccac cggcgtcggc gtacgtttaa tgaataaaga
    45781 tcaaagcaat atcgttttag gcaccgctac tccagatatc gacctggctc cgacctccag
    45841 cgaacaaacg ctgaatttct ttgcctggat ggaacaaatt gatcaggcta cacctgtaac
    45901 gccaggcgca gttacggcga atgcaaccta cgtgctggat tataaataag tattactttg
    45961 gcggcgctat cgccgccttt cataaaatat ttatcaggta tggacaccgt gaatatttat
    46021 cgactctctt ttgtatcctg cctggtcgtg gcgatgcctt gcgcattggc ggtcgaattc
    46081 aatctgaatg ttctcgataa atcaatgcgc gaccgcattg atatttcatt attaaaggaa
    46141 aaaggagtca ttgctcccgg tgagtatttt gttagcgttg cggtaaataa taaccaaatc
    46201 agtaacgagc aaaagattaa ctggcacaaa aatgacgata aaaccattcc gtgcatcaat
    46261 gatttactgg tcgataaatt tggcttaaaa cctgaagtcc gtcagtcgtt accattgata
    46321 aatcagtgcg tcgattttag ctcccgacct gaaatgctct tcaatttcga tcaagccaat
    46381 cagcaactga atattagtat tccgcaagcc tggctggcgt ggcactcaga aaactggacc
    46441 ccaccctcca catggaaaga aggtgtcgcc ggtgtcctga tggattacaa cttgtttgcc
    46501 agcagctacc gcccacagga tggcagcagc agcactaatc ttaacgccta tggaacagcc
    46561 ggaatcaaca cgggggcatg gcgcttacgc agtgattatc agttgaatca tactgatagc
    46621 gatgataacc atgaacagtc aggcgaaata tcacgtacct atctttttcg cccgttaccg
    46681 caattaggtt caaaattaac cctcggcgaa acggatttta gtcccaatat tttcgacggt
    46741 ttttcttata ccggcgcggc actggcaagt gacgatcgaa tgttgccatg ggaactacgc
    46801 ggctacgccc cacaaattag cggtattgca cagaccaatg ccacggtgac gatcagtcaa
    46861 tcaggccgcg tcatttacca gaaaaaagtc ccaccaggcc catttatcat tgacgacctt
    46921 aatcagtctg ttcagggcac actggatgtc aaagtgacgg aagaagatgg tcgggtgaac
    46981 aatttccagg tttcagcagc atcgacgccc ttcctgactc gtcagggaca ggttcgctat
    47041 aaactggccg cgggtcagcc acggccctcc atgtcacatc aaactgaaaa tgaaaccttt
    47101 tttagcaatg aagtttcctg ggggatgctg tcaaacacct cgctgtacag cggcctgctg
    47161 ctttctggtg atgactacca ttctgccgca atgggtattg ggcaaaatat gctgtggctt
    47221 ggtgcgctgt cgtttgatgt cacgtgggcc agtagccatt ttgatactca gcaggacgag
    47281 cggggcttaa gctaccgttt taattacagc aaacaagtgg atgccactaa cagcacgatt
    47341 tccctcgccg cttatcgttt ctccgatcgt cattttcaca gctacgccaa ctatctggat
    47401 cacaaataca acgacagcga tgcgcaggac gaaaaacaga cgatcagctt atctgtgggc
    47461 caaccgatta ccccactaaa cctcaatctt tacgccaacc tgctacatca aacctggtgg
    47521 aatgcagacg cctccacgac cgccaacatc acggcaggtt ttaatgttga tattggtgac
    47581 tggagagata tctctatttc tacgtcattc aatacaaccc attacgaaga taaagatcgc
    47641 gacaaccaga tttacctgtc gatttcgctc cccttcggta acggtggtcg ggttggctat
    47701 gacatgcaaa acagtagcca cagcaccaca caccgcatgt cgtggaacga tacgctggat
    47761 gaacgtaata gctggggcat gtctgccgga ctgcaatccg accgtcctga caatggagcc
    47821 caggtgagcg gtaactatca gcacctgagt tcagcgggtg agtgggatat ttctggtacc
    47881 tatgccgcca atgattacag ttccgtcagc agcagctgga gcggttcttt caccgcaacc
    47941 caatatggtg cagcgtttca tcgccgcagc tccaccaatg aaccacgcct gatggtcagc
    48001 accgatggcg tggcagatat tccggttcag ggcaatctcg actacaccaa ccattttggc
    48061 attgcggtgg tgccgttgat ttccagttac cagccttcca ccgtggcggt gaacatgaat
    48121 gacttacccg acggcgtaac agttgcagaa aacgttatca aagaaacgtg gattgaaggc
    48181 gcgataggtt acaaatcact ggcttcccgt tccggtaaag acgttaacgt catcattcgc
    48241 aacgccagcg gtcagtttcc tcccctcgga gcggatatcc gccaggatga cagcggcatt
    48301 agcgtgggga tggttggcga ggaaggacat gcctggttaa gcggagtcgc tgaaaatcaa
    48361 aagtttaccg tggtctgggg tgatagccag cattgctcgc tccatcttcc tgaacatatg
    48421 gaagacaccg caaatcgcct gattttacct tgtcattaat taaggaaatg aaaatgacat
    48481 ttatgaaagg actacccctt ttgttgttgg ttgccagtct gtgcagccac gctgcactac
    48541 aacccgatcg cactcgtatt gtgtttaacg ctaatgataa agctaccagt ctgcgggtgg
    48601 ataatcgtag cgataaactc ccctacctcg cttattcctg gctcgaaaat gaaaaagggg
    48661 agaaaagcga tgatcttctg gtcgccctgc cgccgataca acgtctggaa ccgaaagcaa
    48721 caacacaggt acggatagta aagcaagcct cgaccacaaa attaccgggc gatcgcgaaa
    48781 cgctattttt ctacaatatg cgtgaaattc ctcccgcccc ggaaaaaaac agcgaccatg
    48841 ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt ctggcgaccg gctgcattac
    48901 gcaagaaagc aggagaaaag gtcgaactgc agttacaggt cagccagcag ggcaaccaac
    48961 tgacactgaa aaatcctacc gcgtattatc tgaccattgc ctacctggga cgaaatgaaa
    49021 aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc ttttagtacg gtcaacacaa
    49081 acaccggaaa ttatagcggc agccagtttt atctcggtta tatggatgat tacggcgcgt
    49141 tacgcatgac gacgctcaac tgcagcggac aatgccattt acaagccgtg gaggcgaaga
    49201 aatgagtgct ggcaagggag tgttgctcgt catctgtctg ttatttctgt cgttgaagtc
    49261 tgcgcttgcc ctgaactgct actttggttc atccggtggc tcggtagaaa aatcagaagc
    49321 gattcaacct tttgctgtac caggcaatgc caaactcggc gataagatct gggaatctga
    49381 cgatattaaa ataagatctg ggaatctgac gatattaaaa ttcccgtcta ttgtgacaac
    49441 aataccaacg gcaactttga aagcgaacat gtctacgtat gggtaaaccc ctacccgggg
    49501 gtgcaggatc gttattatca actcggagta acttacaatg gcgttgatta tgacgccaac
    49561 cagggaaaaa gccgcatcga cactaaccag tgtatcgaca gtaagaacat tgacatttat
    49621 acccctgatg ggatggcaga acaaaatttg ctcgggtgat cccgctaata ttcacatgtc
    49681 gcgaaccttt ctcgctcgca tgcggttata cgtcaaaata cgagaaatgc cgccgcatga
    49741 ttatcaaagt acgcttagcg actatatcgt cgtgcaattt gacggtgccg gtagcgttaa
    49801 cgaagaccct actgcccaaa acctgaaata tcatattact ggtctggaaa acattcgcgt
    49861 gctggattgc agcgtcaatt tttccatttc accggaaaca cagatgattg attttggtaa
    49921 atttaatttg ctggatattc gtcgccacac aatgtcgaaa acattcagta ttaagacgac
    49981 aaaatcacaa aatgaccaat gcaccgacgg atttaaagtc agttcctcct tttataccga
    50041 agaaacgttg gttgaagaag ataaagcatt gttgattggt aatggtctga agctacgttt
    50101 gctggatgaa aacgcctcgc cttatacctt caataaatat gctgaatacg ccgatttcac
    50161 cagcgacatg ttggtctacg aaaaaaccta tacagctgaa ctttcgtcca ttgcaggtac
    50221 ccccatcgag gctgggccct tcgatacagt ggtgcttttt aaaattaact ataactgatt
    50281 tgccatgatg ccctttgcag ccggaaaggg catctctttc agaaaattgc tgattttccc
    50341 cttttccttg attgctgtta ttttttccta gcccggcttt ctttatgctg tatcgataca
    50401 ccaaagagaa aataatgagg gagcgaagga tgccagtttt gcagtgggga atgttatgtg
    50461 tgctatcact tctcctttct attggtttcc tcgcggtaca tctcccggcg gcgctattac
    50521 ttgggccgat gatcgccggg atcatcttca gtatgcgcgg aattactctg caacttcccc
    50581 gctccgcttt tctcgccgcg caggccattc ttggctgcat gattgcgcaa aacctcactg
    50641 gttcgattct caccacgctg gccgccaact ggccgatcgt gcttgctatt ttactggtga
    50701 cgctgctttc cagcgccatc gtgggttggt tattggtgcg ctatagctca ctgcctggaa
    50761 ataccggtgc ctggggttct tctccaggcg gcgcagcggc aatggtcgct atggcgcaag
    50821 attacggcgc agatattcgc ctggtggcgt ttatgcaata tctgcgagtg ctgttcgtcg
    50881 cgggggccgc tgttctggtc acccgcatga tgctgggtga taacgctgaa gcggttaacc
    50941 agcaaatcgt ctggttcccg ccagtgagca ttaatctcct gcttacgatt ttgctcgcag
    51001 tcgttgccgg tacggcggga tgcatgctgc gtcttccttc cggcacgatg ctcatcccga
    51061 tgctggcggg cgcagtgctc cagtctggtc agctcatcac catcgaacta ccggaatggc
    51121 tgctggcgat ggcgtatatg gcaattggct ggcggattgg tcttggtttc gataaacaaa
    51181 tcttactgcg ggcactacgc ccgctaccgc aaatcctgct gtcgattttt gctctgctgg
    51241 ctatttgtgc aggtatggcg tggggactga cccggtttat gcatattgat tttatgactg
    51301 cctacctcgc caccagtccc ggcgggcttg ataccgtggc ggtcatcgcc gcagggagca
    51361 atgccgatat ggcgcttatt atggcgatgc aaaccctgcg cctgttcagt attttgctga
    51421 cggggccagc cattgcgcgg tttatttcaa cctatgcgcc gaagcggtcg gcctagtgct
    51481 ggcagcctgg acaccagtaa aacgggcgcg aggacaacgt ggttttctca atgatgccgc
    51541 cacagcgttc gcacagttcg ccatcacgat gaaaaacctt aaagcgaaac agcgccccat
    51601 gatgcttatt ctcatccacc tgcccccgcg tagcgtaaga aaaccgagga atctccagta
    51661 acgcgtgtgc gagtgcatcc agttgcgccg cattgagatc tttcgcttta tgatttccag
    51721 tcaacccaac ctgccagagg atctccaccc gcaaataatt gccaagccca gccagaaacg
    51781 cctgatcgag cagtaatcct gcaaactgac ggttacgaaa gcgcggcgac aataaccgtt
    51841 cctttaccac ctccggcgtc agattcggat ccagcacatc gggaccaacg cgttgtaaaa
    51901 acggatgcgt ggtcagttgt tccggcctca acatctcaat atccgaggcg ctataaagca
    51961 gaatggtttt gtcagccgtt tgcagtttta cccgcaatac tcgcgtggtc tgcggctctt
    52021 cgccggtatc aaccacgcgc cagacgccgt aaagctgatt atggctgtag agcgttaaac
    52081 cgttgggaaa atgagttaac aacgccttac cacgcgtttc cacatgggta acgtgttgac
    52141 cgataagttg tgattgataa gtttttaact gcgggaaggc aaaccagaca tcagttagtg
    52201 gtttgccttt gatcgccgcc tccaggttat ccgctgcacg gcggatctcc gggccttcag
    52261 gcatggtgat atcctttttt cattctttat taagcggggt tatgctgcga caacaatccc
    52321 ctttttggca aatgtagctc gtaatcggcg ggcgaacgcc agagcgtgct cgccgtcgcc
    52381 atgcaggcag accgtttgcg ccgcgaccgt tgcccattcg ccggtgatac ttttgactct
    52441 gccgtgttgc accatctcca gcgtttgcgc caacgcctgt tcttcgtttt caatcaacgc
    52501 gcctggctga cttcgcggca ccagcgagcc gtcagcctga taaccgcgat cggcaaacac
    52561 ttcctcgcgc gttgtcaggc catattgctt gcctgcacga atcagctcgc ttcctgccag
    52621 cccaacgaga accaatgccg gatcgcaagc gtataccgct ctggcgatgg cgtctgccag
    52681 ttgtgcttct ttcgccgcct ggttgtacaa catgccgtgc ggtttgacat gacgcattac
    52741 gccgccttgc gcacgggcaa tcgttgccag cgcgccaatt tgatacagcg tctgggcgta
    52801 aacggtttcc ggcggcagct gcatggcgct gcgaccaaaa ttttccctgt cgggaaaact
    52861 cgggtgagcg ccaatcgcga caccattttt tattgcttca cgcacgcaag cctgcatggt
    52921 ttgcgcatcg cctgcatgaa atccacaagc aatattggca gaggaaacca gcgttaatag
    52981 ctctgcgtcg ctggcgcagc cttcgcccag atcggcgttc aggtcaattt tcattgtgca
    53041 gccgccacgc taattgttca aaataacgtt gctgatcttg ccgcgctttc agtgcctctt
    53101 ccagtgaaca ctggacaaaa tgaatcggct gaccgagcgg aatttgcgcc agatggtaca
    53161 tatcagcctc aatgatacag gcaatacgtg gataaccacc ggtggtctgt gcgtcgttca
    53221 tcaacacaat cggctgcccg ttatgcggca cctgcaccac gcccggtaac aaaccgtgag
    53281 ataacagttc gcgatcggtg atgcgtttta aaatttgccc ctgtaaacga tagcccattc
    53341 ggttactttg cgagctaagc tgccagggcg aacgccagaa tgcatcctgc gaggcgcgat
    53401 cgaactcatg atattccggc cccggcaagg cgcgaatgcg gttgccccac agcagctgtt
    53461 taacgccctg cgcttccata aaatcacgct tcgatttgcc aatcgggagt cagtcaccat
    53521 ccttcagtaa acggccttcc agcccgccaa tccccacttt gagatcggtg ctgcatgacc
    53581 ccattaccgg cggaacatca ataccacccg cgaccgccag ataactgcgc atcccgtgct
    53641 gcgggcgttt aagcgttaaa cgctggcctg ctttcatcgg caatcgccag ccggtccaga
    53701 cggcgttatc atccaaccgc gcttcgcaac ctgcacccgt cagggcaaac cacccatcag
    53761 tttcgaactc aactgttaac tgaccgagcg tgatctccag cgcgggggca ttggcgtcat
    53821 tacccaccag caggttagca atgcgcagcg caggcatatc cagtgcgcca cagtggctga
    53881 tacccgactg gcaaaaaccg tgacgaccgc catcctgcac agtggtgtac atgcccgcac
    53941 gaataatctt cagcatattc cctccttctg cggtacaaat cgcacgctgt ctcccggacg
    54001 taataagatg ggttcgtcac gcgccggatc aaacaggctg agtgaggtat gaccaatcaa
    54061 ctgccagcca cccggcgttg ccagcggata aacaccagtc tgcggcccgc cgatcccgac
    54121 agaacctgcc ggaacgagca agcgcggttc agcgcgccgt ggcgtgtgta gttgttccgg
    54181 caaactcccg agatacggga agcccggttg aaaacctaaa aaccagacca cgtattccac
    54241 ggaggagtgc aattcaacaa cctgtttttc gctcaacccg caatacgccg cgaccaccgc
    54301 caaatccggt ccgcctgcac caccgtaaac caccggaatt tcaataaagc gagactcagg
    54361 ttccagggcc tcgctctcct cccaccagcg ttgcaaacgc tctatggcat ccagcgccag
    54421 cgactcagga ttacgcaaaa tcaccgtgat attgttcatg ccgggaatgg cttcaaccac
    54481 attcggcata tccaccagac gctgcgccag tcgccagatc cgtttctggc tagccagcgt
    54541 caccggcggt tccagttcca gcactaccgc cgtttcacct atcagataac aacgcgctcg
    54601 ttgcactttc gatccctctg attattcgtt atgcaggatt aggaatatca ataaaggtca
    54661 catcaagatc ggtattttca ttcagccact cgctcaatgc gcgaatacca ccgcgttcag
    54721 tggcatggtg acctgcagca taaaaatgca atccctgctc gcgggctgaa tgaatggtct
    54781 gttcagaaac ttcgccagta ataaaagcat ccacgccaaa acgcgcggcg ctatcgataa
    54841 aactttgccc gccgcccgtg caccaggcga cgcgctggac cacctcaggt ccggtatcgc
    54901 cacaccataa cggcttacgt cccagacgcg cttcaatcca ggaggctagt tccattcccg
    54961 gcacaggcat ggtcagctct ccccacggca ccaacggctc aatttcgccc atgaccgtga
    55021 tccccagtaa cgccgccagc tgtgcgttat tgcccagctc aggatgtgcg tcaagcggca
    55081 aatgccagcc atacaggttg atatcattcg ccagcaacgt ttttaaacgg ttacgcttca
    55141 ttccgcgaat gaccggagac tctcctttcc agaagtagcc atgatgcaca atgaccgcat
    55201 ccgcgcccag acgcactgcc tcatcgagca aagcctggct ggcggtgaca ccggtaacaa
    55261 ttttttgcac cgtctctttg ccttccacct gcaaaccatt cggcgcgtaa tcactaatcg
    55321 ccgcgctgtt cagtttttcg ttaatcagtt gttccagttc ggtgtttttc atcttcgcgc
    55381 cttccgtatt ctgatagcta caccgttaac atagcgttac atcgcctgtt cgtcgatagc
    55441 caatcttttg acatattaag aaacaaaccc gacgccaaaa gacgcaaatt gtttatatca
    55501 tcacgcttca gctaatgtat atttatgcaa tcagacagca tattgcataa ataatcagat
    55561 ctgtatcaaa gttttaactt taaggaaaca caagcacgcc gcgcaactat tacgtgacgt
    55621 tgccgtcaat tgtggaatta ttatgaataa acacgcatca caaccgcgcg ctatttacta
    55681 tgtcgttgcg ctgcaaatct gggaatattt tagcttttac ggcatgcgtg ccctgctgat
    55741 tctctatctc accaatcaac taaaatacaa cgatactcac gcctacgagt tatttagcgc
    55801 ctactgttcg ctggtgtatg tcacgccaat cctcggtggc tttttggcgg ataaagttct
    55861 cggcaatcgc atggcggtga tgctgggggc gttgttgatg gcgatcggtc atgtggtgct
    55921 gggtgccagt gagatccatc cgtcattcct ctatctgtcc ctggcgatta tcgtctgcgg
    55981 ctatggcctg tttaaatcta acgtaagctg tctgctcggc gagctgtatg agccaaccga
    56041 tccgcgtcgt gatggcggtt tctcgctgat gtatgcggcg ggtaacgtgg ggtctattat
    56101 cgcacctatc gcctgtggtt acgcccagga agagtacagc tgggcgatgg gctttggcct
    56161 ggcggcggtt ggaatgatcg cgggtctggt cattttctta tgtggcaatc gtcatttcac
    56221 tcatacccgc ggcgttaaca aaaaagtact gcgtgcgaca aactttctcc tgccgaactg
    56281 gggatggctg ctggttctgc tggtggcaac gcctgcactg attaccgtgc tgttctggaa
    56341 agagtggtcg gtatacgcct taattgtcgc gactattatt ggcctgggtg tactggcaaa
    56401 aatttatcgc aaagcagaaa accagaaaca gcggaaggag ctggggctga ttgtgacgct
    56461 caccttcttc agtatgttgt tctgggcctt cgcacaacag ggcggtagct cgattagcct
    56521 ttatatcgac cgcttcgtta accgcgatat gtttggttat accgttccga ccgcgatgtt
    56581 ccagtcgatt aatgccttcg cagttatgct gtgcggtgtg ttcctggcgt gggtggttaa
    56641 agaaagcgtc gcaggtaatc gtaccgtgcg catctgggga aaatttgctc ttggccttgg
    56701 cctgatgagc gccggattct gcattctgac cttaagcgcc cgctggtccg caatgtatgg
    56761 tcactcttct ctgccactga tggtgttagg cctcgcggtg atgggctttg cggaactgtt
    56821 tatcgacccg gttgccatgt cgcaaattac gcgcattgaa attccgggcg tgaccggcgt
    56881 attaaccggc atctacatgc tgctttctgg cgcgatcgcg aactatctgg cgggcgtgat
    56941 tgccgatcag acatcgcagg cttcgtttga tgcttccggg gcgatcaact actccatcaa
    57001 tgcatatatt gaagtatttg atcaaattac ctggggcgca ctggcgtgtg taggactggt
    57061 actgatgatt tggctgtatc aggcgctgaa attcagaaac cgcgcgctgg cgctggagtc
    57121 ataataggcc agcaccgatg cctgatgcga cgcttgacgc gtcttatcag gcctacgtat
    57181 ttcctgcaat ttattgaatt tgcacaaatt tgtaggctgg attaggcgtt cacgccgcat
    57241 ccggcatctg ggctcgattg cctgacgcgt ctgttatttc cccttccgcg ccgcctcata
    57301 cgctgccaac gtttgtactc tcgcctcttt gtgctcgact atcggttgcg gataatccag
    57361 cttcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc
    57421 gcgcagctcc ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt tctcgccctg
    57481 agttatcgga ttgaaaatac gaaaatacgg cgcagcatca gtacccgtag aagccgccca
    57541 ctgccagcca ccgttattag ctgccaaatc accatcaatc agctgcgaca tgaaatatcg
    57601 ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg
    57661 tagccggtta tgcatccagc cagtgctgtt aagctgacgc atagcggcat caataatcgg
    57721 gtatcccgtt ttgcctttct gccaggcctg taaatgtgcg ggattgcttt gccactgcac
    57781 acgatccgtc caggcaataa atggacgatg tttacacaac gaggggtaat acgtcatcag
    57841 atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc
    57901 cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc
    57961 cgttgccaag ctggcggaca aacggctggt gccttccact gccggaaaat ctcgttgttg
    58021 ctcatactct ccagcaccgt tctcgcaaaa ctggcgtaat tgcgcaatcg ccgctttttc
    58081 ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg
    58141 cgcgggcttt atcgatccgc tactacgaac ttttggtgca gcgacgcact ccggcatccc
    58201 ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg
    58261 attgccggtc atcaccgccc ccggcggcag gatcacgctg tcatcaaatc cttcacacac
    58321 cacgttacgc agcgctcttt caacgtccac atcccgcgct cgctcattca cctcatactg
    58381 atagttataa aaaagatgag taacgctgtt ttccgcgcac acctgtttaa ctatttcgac
    58441 actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt ccgccagcgc
    58501 gatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg
    58561 cgtctcccac ttgcgtggtg tagcgatata cagcgccagc acgcgtgcag acgaattgcg
    58621 gcaggcggca gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accaggccag
    58681 atgggtagtc ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa
    58741 taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc
    58801 agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc agccagctgc
    58861 tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacgttg
    58921 gtgtgatcgc gcgggttggc gggatgagaa agcaaaacaa ttacgcgttg gcggtactgg
    58981 ttgtaaaaat catccagatt ttgccactga tgaatatcag ccacaaacgg gccaagtttg
    59041 cggtactcgg gctgagaatg ggccagaaag acgagtttat agcggctgtg ataatcgaga
    59101 agcgccccgc gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcgaagata
    59161 cggattaggg tgctgtcatc aaatcgttgt agattcatta ctgctcctga acggtataca
    59221 ggagtaagtg taggcgttgg taaatgatgt ttgtgaagtt aatccgctag cagggcaaga
    59281 ttacgaaagc gcgctccccg caagcaacta atcaaggtag ttaatcatgg ctgaacacga
    59341 tattacgcca gaagtcacta tttccaaaac acagcgccgc tataaagtcg gctatgtcag
    59401 agcccgccac caggaccgca aaaccggtat agcccggtat tattcacaac atcccagtct
    59461 gcaccttaag ggcgactggc tgaaagaggc cggatttgat accggacggg gtgtcacggt
    59521 gaagatttcg gaggggggcc tgaccatcat cgctgacagt gacgaggtgc aggaactgcg
    59581 ggatgggatg tttagtgtgc tgaacgaaag ttaaatatta aaaaagctgg gaagatttaa
    59641 aaccttccta gctttttatt ctgtcttatt ataatggata ctcatcatct tccgatgaat
    59701 ttataaattg ctccaagttt acatgaagag tacttaacct agtttttaac tcattaagtg
    59761 aagcgataat ttcatatttt ccaacataat catatgatgg atcgtattca ttatctatgt
    59821 cagaagctaa aaatgatgat attcgtagtt taaatttagc ttcatcttta ttacattcat
    59881 tggcataagg tgaatgccct atcatagcat attcatctaa tctaaaagga taagatggtt
    59941 ttaattcatc gatcaatgaa atttcaatcc atgttctata tccaggcatt aatggactaa
    60001 tttctttgag caaaaccttt ccacttaata atgtattgag attgttcatt attatgtctc
    60061 ccacgggaaa aatatatgtc taacacttcc accatgttga tgaacttgta aatgtggtaa
    60121 atcaccatca ggatttcctg gcccatgacc tgacactcta tatgaaggtt ttgttacaga
    60181 aggatctttt atatcatccc aatgatatga acctgctttt gttttatttt cagtaagata
    60241 ttccattaag ttcttagtgc tggggcctga ctcacctgtc atatttttat aattatgacc
    60301 taaatatcta tccataaaca attgttcagc gtcacttcta gacctaacag taactttggt
    60361 ttcactacta tctaataaac gattcgcttt ttggttacta acagcacatg gagttaatcc
    60421 aagtggatca gcatatttta gaggattagg tgcataactg tagagattta aaccgcctct
    60481 caaccctatc gggtcagtta ccatgaaccg cccgacatcc ggatcatagt acctgtgcag
    60541 attgtagtgc agccccgttt ccctgtcgag gtactgtccc tggtagcgca ggttctgctc
    60601 caccccgccg tggtccttct cctgtatcgt gttaccccag acctgatacc ccgtctccca
    60661 cacgattttc ccgtcgctgt ccgtcatctc ttccggcgcg ccgttcacat ccgtgtgaaa
    60721 atacagtatc cggttcgggc cctctttgcc cgccttatcc acccgcgcca gcggttcata
    60781 actcccctgg tccctgtaga tgtacaggct gcctgttccc tccggcctgc tctcctgcgc
    60841 cagccgcatt ccgtcccata aaaattttgt ccggttgtac ggcttaccgt cagggctgat
    60901 gtgctgtttt tccgtccgcc tccccagtgc atcgtacaca taccggtatg tcccccgcgc
    60961 tgtcgtcact tccaccatcc ggtgttccgc atcataacgg taactctgcg ttccgctccg
    61021 gccctccttt tccaccgcac ggccaaattc gtcataacgg tagtgcacac cacggtaaga
    61081 catcagccgg ttgaacggca ccacgctgtt ttctgccgtg gccctttccc ccgcgcgcct
    61141 ccccagcagg tttcccgccg cgtccacctc cgtttccggc tggtgccaca ccgggtgcca
    61201 ctgcgcctgc tccacccgcc ccagcgggtc gtgcgtctcc gtgatatgcc cctgcaggtc
    61261 atacacatag cgccatttgc cgccctgagg gtcggtcatc cccagcaaca gtcgttcttc
    61321 gtcgctccag cggaatgtcg tcacctgtcc ggcttcgtcc tggtacgccg tcacctgatt
    61381 ctgcgcatcc cactggtgat aacgtgtgcg ccccagtccg tccgtcacgc acagcctgcg
    61441 ctgcgccagg tcccacgcca gccggtactg ttcaccgctg tccgtgtggt gccccgtcac
    61501 ccgccatgcc gcgtcatccg gaccttcata ccagccccac tggtaataat acccttcgcc
    61561 gcccggctgg cggtgcagcg tcatgcagtg gttttcgtcg taagcaaact cacgcagcag
    61621 gtgccccgtg ctgtcgtaca cgccgttcag gtggccggcc tcgtcatagc tgtaacgacg
    61681 cagcagtaaa ggtgcagtct cagggaaatg ctgataaatc tgtgtcaccc gccggggatg
    61741 cgccgccagt tcgtaataca gacggatgca cggacgctgc tgttcgccgc tgatttccac
    61801 aatgcggccc cggtcgtcgt agtacagatt cagacagttg ctgttacggt cgcccagcat
    61861 tttcagccgc tggcgctgcg ggtggtgcgg gtcagcctca aacagaaaac actgcccgtc
    61921 atcgctgctg ataagccagt gcccctgctc accacgcctt accgccagcc cttcaccggg
    61981 gctgtaaaac ccgccccccg gcagtacggc ttccatatcc aagcgtcgcc ccatgcccga
    62041 cacaaaggtc atgcagtttt cgtctgcgct acccggtgtg cacacgaggc tgacttcata
    62101 cattacactc cagccctgac caaacattcc ttccgtacgt tcgtcacgac tgctgtacac
    62161 gcgctgccag tccagcggaa agtggcccgg cagactgaaa tcgaagtcat cgtcatcggc
    62221 caggtatttg gccccggtgg cggcattgac cggatgcgga agggcactgg tggccttctg
    62281 cgtcagcatg ctcatcccga cactggcagc aaaacaggcg attttagaaa gcattttgcc
    62341 cgggcgaaga aacatcagcg ccacggttgc caccagggtt atctgcgacc tgccgcttct
    62401 gatatccctg accaccagta acggaccgcc aatgcgcaca tccggtgaca caaacgcccc
    62461 gttccccggc tcattcacca ccctggcctc gcaggtacag cgtgccccgc tgcgtgtcgc
    62521 cggctggctg ttgatacgca catcccgcgc gccttccgcg atatattccg ctgcgggcgg
    62581 ctcgtcatcg cctttgaaca gattactgat gctgccaata accgcattca ctccctgaaa
    62641 ggcggtttcc agcataccgc ccggattcag caggttaagg gccgttccca gggggtcatc
    62701 cagaaaggcg gctttttttt gtgcgagaaa ctgcgtcagt gaggaaggat gtttatggca
    62761 gtcgatttta tcgtcagggg tcggggtgga tcccggggcc gcactggcca cagtgggctg
    62821 ccatagttcc gacacaaaat gtgtcacgcc atccaccatt tcagaacgcc cttcttccgt
    62881 actgagctgg tcgtgcagag cccgggacat cagcacaggc cctgtcaccg ggttaaccgc
    62941 cagggctatg gcccccgcca tttccagata ttccccggcc acatcccgga acgttctggc
    63001 atcctcctga cgctgcgctt cttcctgttc tgccttcgcc tgcgcttcct gcgcctctat
    63061 gtcctgccgc gatgccgcag tcgccgctgc ccgcgcggca ggtttgctgt tgatccatgt
    63121 gtcaccagag ccggtgagga tgtaaccttc aatctgaggc gggaaaag
//
LOCUS       DAJTYM010000010        60288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTYM010000010 DAJTYM010000000
VERSION     DAJTYM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..190
                     /locus_tag="OO575_000647"
     CDS             <1..190
                     /locus_tag="OO575_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCR6663726.1"
                     /translation="SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTI
                     PIEAGEFKATSTFQVTYP"
     gene            303..1097
                     /gene="panB"
                     /locus_tag="OO575_000648"
     CDS             303..1097
                     /gene="panB"
                     /locus_tag="OO575_000648"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6663727.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1109..1960
                     /gene="panC"
                     /locus_tag="OO575_000649"
     CDS             1109..1960
                     /gene="panC"
                     /locus_tag="OO575_000649"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6663728.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2034..2936)
                     /gene="rpnC"
                     /locus_tag="OO575_000650"
     CDS             complement(2034..2936)
                     /gene="rpnC"
                     /locus_tag="OO575_000650"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6663729.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3210..3590
                     /gene="panD"
                     /locus_tag="OO575_000651"
     CDS             3210..3590
                     /gene="panD"
                     /locus_tag="OO575_000651"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6663730.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3594..4823)
                     /locus_tag="OO575_000652"
     CDS             complement(3594..4823)
                     /locus_tag="OO575_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6663731.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(4887..5327)
                     /locus_tag="OO575_000653"
     CDS             complement(4887..5327)
                     /locus_tag="OO575_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6663732.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5432..6202)
                     /locus_tag="OO575_000654"
     CDS             complement(5432..6202)
                     /locus_tag="OO575_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6663733.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6199..7125)
                     /locus_tag="OO575_000655"
     CDS             complement(6199..7125)
                     /locus_tag="OO575_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6663734.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7234..7896
                     /gene="can"
                     /locus_tag="OO575_000656"
     CDS             7234..7896
                     /gene="can"
                     /locus_tag="OO575_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6663735.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7937..8473)
                     /gene="hpt"
                     /locus_tag="OO575_000657"
     CDS             complement(7937..8473)
                     /gene="hpt"
                     /locus_tag="OO575_000657"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6663736.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8679..11069
                     /gene="gcd"
                     /locus_tag="OO575_000658"
     CDS             8679..11069
                     /gene="gcd"
                     /locus_tag="OO575_000658"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6663737.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11116..12666)
                     /gene="cueO"
                     /locus_tag="OO575_000659"
     CDS             complement(11116..12666)
                     /gene="cueO"
                     /locus_tag="OO575_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6663738.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12832..13179
                     /locus_tag="OO575_000660"
     CDS             12832..13179
                     /locus_tag="OO575_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6663739.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13285..14151
                     /gene="speE"
                     /locus_tag="OO575_000661"
     CDS             13285..14151
                     /gene="speE"
                     /locus_tag="OO575_000661"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6663740.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14167..14961
                     /gene="speD"
                     /locus_tag="OO575_000662"
     CDS             14167..14961
                     /gene="speD"
                     /locus_tag="OO575_000662"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6663741.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(14999..15361)
                     /locus_tag="OO575_000663"
     CDS             complement(14999..15361)
                     /locus_tag="OO575_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6663742.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15536..18133)
                     /gene="acnB"
                     /locus_tag="OO575_000664"
     CDS             complement(15536..18133)
                     /gene="acnB"
                     /locus_tag="OO575_000664"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6663743.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18488..20341
                     /locus_tag="OO575_000665"
                     /pseudo
     CDS             18488..20341
                     /locus_tag="OO575_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20412..21836)
                     /gene="lpdA"
                     /locus_tag="OO575_000666"
     CDS             complement(20412..21836)
                     /gene="lpdA"
                     /locus_tag="OO575_000666"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6663744.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22161..24041)
                     /gene="aceF"
                     /locus_tag="OO575_000667"
     CDS             complement(22161..24041)
                     /gene="aceF"
                     /locus_tag="OO575_000667"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6663745.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24056..26719)
                     /gene="aceE"
                     /locus_tag="OO575_000668"
     CDS             complement(24056..26719)
                     /gene="aceE"
                     /locus_tag="OO575_000668"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6663746.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26880..27644)
                     /gene="pdhR"
                     /locus_tag="OO575_000669"
     CDS             complement(26880..27644)
                     /gene="pdhR"
                     /locus_tag="OO575_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6663747.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28185..29558
                     /gene="aroP"
                     /locus_tag="OO575_000670"
     CDS             28185..29558
                     /gene="aroP"
                     /locus_tag="OO575_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6663748.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29601..30455)
                     /gene="ampE"
                     /locus_tag="OO575_000671"
     CDS             complement(29601..30455)
                     /gene="ampE"
                     /locus_tag="OO575_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6663749.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30452..31003)
                     /gene="ampD"
                     /locus_tag="OO575_000672"
     CDS             complement(30452..31003)
                     /gene="ampD"
                     /locus_tag="OO575_000672"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6663750.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31091..31984
                     /gene="nadC"
                     /locus_tag="OO575_000673"
     CDS             31091..31984
                     /gene="nadC"
                     /locus_tag="OO575_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6663751.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32187..32627
                     /gene="ppdD"
                     /locus_tag="OO575_000674"
     CDS             32187..32627
                     /gene="ppdD"
                     /locus_tag="OO575_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6663752.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32637..33784
                     /gene="tadA"
                     /locus_tag="OO575_000675"
                     /pseudo
     CDS             32637..33784
                     /gene="tadA"
                     /locus_tag="OO575_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33774..34976
                     /gene="hofC"
                     /locus_tag="OO575_000676"
     CDS             33774..34976
                     /gene="hofC"
                     /locus_tag="OO575_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6663753.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35011..36054)
                     /gene="guaC"
                     /locus_tag="OO575_000677"
     CDS             complement(35011..36054)
                     /gene="guaC"
                     /locus_tag="OO575_000677"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6663754.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36216..36254)
                     /locus_tag="OO575_000678"
     CDS             complement(36216..36254)
                     /locus_tag="OO575_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663755.1"
                     /translation="MIIKTLKMSARK"
     gene            36279..36899
                     /gene="coaE"
                     /locus_tag="OO575_000679"
     CDS             36279..36899
                     /gene="coaE"
                     /locus_tag="OO575_000679"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6663756.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            36899..37642
                     /gene="zapD"
                     /locus_tag="OO575_000680"
     CDS             36899..37642
                     /gene="zapD"
                     /locus_tag="OO575_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6663757.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37652..37849
                     /gene="yacG"
                     /locus_tag="OO575_000681"
     CDS             37652..37849
                     /gene="yacG"
                     /locus_tag="OO575_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6663758.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(37949..38338)
                     /gene="mutT"
                     /locus_tag="OO575_000682"
     CDS             complement(37949..38338)
                     /gene="mutT"
                     /locus_tag="OO575_000682"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6663759.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38398..41103)
                     /gene="secA"
                     /locus_tag="OO575_000683"
     CDS             complement(38398..41103)
                     /gene="secA"
                     /locus_tag="OO575_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6663760.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41165..41677)
                     /gene="secM"
                     /locus_tag="OO575_000684"
     CDS             complement(41165..41677)
                     /gene="secM"
                     /locus_tag="OO575_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6663761.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(41908..42825)
                     /gene="lpxC"
                     /locus_tag="OO575_000685"
     CDS             complement(41908..42825)
                     /gene="lpxC"
                     /locus_tag="OO575_000685"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6663762.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(42926..44077)
                     /gene="ftsZ"
                     /locus_tag="OO575_000686"
     CDS             complement(42926..44077)
                     /gene="ftsZ"
                     /locus_tag="OO575_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6663763.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44138..45400)
                     /gene="ftsA"
                     /locus_tag="OO575_000687"
     CDS             complement(44138..45400)
                     /gene="ftsA"
                     /locus_tag="OO575_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6663764.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45397..46227)
                     /gene="ftsQ"
                     /locus_tag="OO575_000688"
     CDS             complement(45397..46227)
                     /gene="ftsQ"
                     /locus_tag="OO575_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6663765.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46229..47149)
                     /gene="ddlB"
                     /locus_tag="OO575_000689"
     CDS             complement(46229..47149)
                     /gene="ddlB"
                     /locus_tag="OO575_000689"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6663766.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47142..48617)
                     /gene="murC"
                     /locus_tag="OO575_000690"
     CDS             complement(47142..48617)
                     /gene="murC"
                     /locus_tag="OO575_000690"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6663767.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48671..49738)
                     /gene="murG"
                     /locus_tag="OO575_000691"
     CDS             complement(48671..49738)
                     /gene="murG"
                     /locus_tag="OO575_000691"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6663768.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49735..50979)
                     /gene="ftsW"
                     /locus_tag="OO575_000692"
     CDS             complement(49735..50979)
                     /gene="ftsW"
                     /locus_tag="OO575_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6663769.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(50979..52295)
                     /gene="murD"
                     /locus_tag="OO575_000693"
     CDS             complement(50979..52295)
                     /gene="murD"
                     /locus_tag="OO575_000693"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6663770.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52298..53380)
                     /gene="mraY"
                     /locus_tag="OO575_000694"
     CDS             complement(52298..53380)
                     /gene="mraY"
                     /locus_tag="OO575_000694"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6663771.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53374..54732)
                     /gene="murF"
                     /locus_tag="OO575_000695"
     CDS             complement(53374..54732)
                     /gene="murF"
                     /locus_tag="OO575_000695"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6663772.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54729..56216)
                     /gene="murE"
                     /locus_tag="OO575_000696"
     CDS             complement(54729..56216)
                     /gene="murE"
                     /locus_tag="OO575_000696"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6663773.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56203..57969)
                     /gene="ftsI"
                     /locus_tag="OO575_000697"
     CDS             complement(56203..57969)
                     /gene="ftsI"
                     /locus_tag="OO575_000697"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6663774.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(57985..58350)
                     /gene="ftsL"
                     /locus_tag="OO575_000698"
     CDS             complement(57985..58350)
                     /gene="ftsL"
                     /locus_tag="OO575_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6663775.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58347..59288)
                     /gene="rsmH"
                     /locus_tag="OO575_000699"
     CDS             complement(58347..59288)
                     /gene="rsmH"
                     /locus_tag="OO575_000699"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6663776.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59290..59748)
                     /gene="mraZ"
                     /locus_tag="OO575_000700"
     CDS             complement(59290..59748)
                     /gene="mraZ"
                     /locus_tag="OO575_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6663777.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14426 a        16708 c        14819 g        14335 t
ORIGIN      
        1 ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa caggaggagt
       61 ttacccggat caggatacag gtataacata ccctctccat ttccaggcca cgctacaaca
      121 ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt tccaggtaac
      181 ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt gataaatcat
      241 tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta tcaggatacg
      301 ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa aagcgtttcg
      361 cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa gggcttaacg
      421 tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc actctgccag
      481 taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca ccaaactgcc
      541 tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc tttgaaaacg
      601 ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt gagtggctgg
      661 tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac ttaggtttaa
      721 ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc aatgaagcga
      781 gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag ctgctggtgc
      841 tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg atcccggtta
      901 ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac gacgcctttg
      961 gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa acgggcgaca
     1021 tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat ccgggcgaag
     1081 aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc cgctgctgcg
     1141 tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc ctactatggg
     1201 taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg ccgatgtggt
     1261 cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc tggcacgtta
     1321 tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg atttagtttt
     1381 cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt acgttgacgt
     1441 tcctggcctt tcgaccatgc tggaaggtgc cagccgtccg ggacattttc gcggcgtttc
     1501 gactattgtc agcaagctgt tcaacctggt ccagccggac atcgcctgct tcggtgaaaa
     1561 agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct tcgatattga
     1621 gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt cccgtaacgg
     1681 ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt taagttcgat
     1741 tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca ttgcggggca
     1801 agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg ccgacacatt
     1861 gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct ggcttggcga
     1921 tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa tacttaactg gcgctacggc
     1981 tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt aacttaaata
     2041 aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc aacgtcttcc
     2101 cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc ctgtcttccc
     2161 tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt catcatagac
     2221 ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg ttcagtatga
     2281 ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt gtacccttcg
     2341 tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg aatatgtttt
     2401 tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc cggtgtgatg
     2461 gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc cggcgagtaa
     2521 aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg ataaaacagt
     2581 atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg catggcagca
     2641 atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg gtgttcaatc
     2701 acaacatgca gataaccggg attgccctgc atttgcatgg aatagagcac gtccgtgctg
     2761 tgacctttca ggcactcttc aatgaaactc cccgactcta aatgaagcgt gttgagatca
     2821 caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc cgtctccgca
     2881 tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc atccatcttc
     2941 cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc tatcgtcagc
     3001 cacctcttta ttgtttaccg agcagcgttc ataaaaatat caattcgtta caggcgttac
     3061 atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga tgagtagaca
     3121 ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac cgcctgcttc
     3181 gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag ggcaaactcc
     3241 accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc attgaccagg
     3301 attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg aatgtcacca
     3361 acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga attatttctg
     3421 ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc gccagcttcg
     3481 ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat tttgaaggcg
     3541 acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga ttgttatccc
     3601 tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct taagatataa
     3661 agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat ggttgtcacc
     3721 gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa gccgccaaac
     3781 ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg tgaacgtgca
     3841 aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg ataaccatct
     3901 acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat ttcgttaagc
     3961 tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg acgtttgatg
     4021 cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac aggatacgca
     4081 tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg cgcagggaga
     4141 ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc gtatcccctg
     4201 tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt cgaagtatgg
     4261 cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac cggcaggcca
     4321 ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat acggatctga
     4381 taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg caaattgtcc
     4441 gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg cgtatctttc
     4501 caggtaatta agttctgatt actcagcggc ttgttgaggt cacccaaacc gtcttcaact
     4561 ttttgtcgtc cctgaactgg ctcgagatgc cctttatcga taaacccttt accaaagcca
     4621 aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg tccggcccga
     4681 atattcccca cggtcaccat cttgtcacta atttgcgccc agaccgcagc attttcgatg
     4741 gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag catcagcagg
     4801 agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg cttttattgt
     4861 agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt gaggacgaaa
     4921 ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc cagttcgaca
     4981 atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat ctgttcaatt
     5041 aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct taataatgaa
     5101 gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc ctcaccagag
     5161 tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct taatccgcgc
     5221 cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag cgcatccagt
     5281 atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc cttcctcctt
     5341 agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt caaaactcct
     5401 ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt ccacgttgga
     5461 tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg ccaaaggtag
     5521 tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg atcatataaa
     5581 cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc agtgagtaaa
     5641 agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg ctgatgtcat
     5701 cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc accgccgtga
     5761 gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca aaaaacagtg
     5821 aaattgccgt cacaagaatg ccaacaaaca gaccacgcgc cacgccaccg ccgacatatc
     5881 cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata ttacgctgga
     5941 acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc accgacatca
     6001 tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg ccaatacgcg
     6061 aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact ggcggcacca
     6121 gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg ctttttagcg
     6181 ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca gtgaaacaaa
     6241 cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc cctgctcact
     6301 taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt ccagcgtcgc
     6361 ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt tcggtgcgag
     6421 atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg aggtattttc
     6481 caccagctca ccatgttgaa taatgccgat attgcggcac agcatttctg cttcttccag
     6541 gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca aaaagcccca
     6601 cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa tcagtagttt
     6661 aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc cagataacat
     6721 acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt tttcgctgcg
     6781 gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca caatttgctg
     6841 cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact gacgtttagc
     6901 gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg aggttttatt
     6961 taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg gcccgagaag
     7021 cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct gaacgccgcc
     7081 tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt tttacttacc
     7141 ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta tattagccaa
     7201 acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac actcatcagc
     7261 aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt tgagaaactg
     7321 gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg cgttcctgca
     7381 gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt tgctaaccta
     7441 gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga tgtactcgaa
     7501 gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc cgccgttgaa
     7561 aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa
     7621 catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg
     7681 aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa
     7741 cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg cttgctgcgt
     7801 gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt
     7861 tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca
     7921 tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac ggcagatgac
     7981 ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac
     8041 cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa
     8101 tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc
     8161 ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct tcatccagat
     8221 ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca
     8281 taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg
     8341 agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa
     8401 tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta
     8461 cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt
     8521 tttggcaccg caacaaagcg caagacgcgc agtataccag taaaactatt cattcgcgga
     8581 atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct ataattaatt
     8641 gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacagcct
     8701 cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac
     8761 tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc
     8821 ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctat
     8881 acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct
     8941 gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt
     9001 ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac
     9061 tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag gagatcaacg
     9121 gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc gatcaggact
     9181 ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag caaattcacg
     9241 ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc
     9301 cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc gatacccttt
     9361 acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc aaagagaaat
     9421 ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc tgccgtggcg
     9481 tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc
     9541 gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct gaaaacggca
     9601 aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca
     9661 ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa accatcgtga
     9721 tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt
     9781 ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg
     9841 caatcccgtc agacgaacac acctttacct ttaactcacc aaactcgtgg gcaccagcgg
     9901 cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg ccggatatct
     9961 ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg
    10021 ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg tgggacatgg
    10081 atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccgatta
    10141 tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat ggcgaactgg
    10201 tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc
    10261 ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata
    10321 tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct
    10381 atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg
    10441 ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc
    10501 caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac ccgatggagc
    10561 agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac
    10621 cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag
    10681 catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta
    10741 ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg ttcaatatgg
    10801 gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta
    10861 cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc
    10921 gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc aagcagtacg
    10981 tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg
    11041 cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc gggcatattt
    11101 ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag
    11161 cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa acttcaccag
    11221 cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc gatgagccgc
    11281 tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg
    11341 cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc
    11401 aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc
    11461 gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg
    11521 gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca tctgcatccc
    11581 catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag
    11641 cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg cactaatagc
    11701 aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatca ccatccccat
    11761 ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac
    11821 ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag
    11881 accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga
    11941 gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg
    12001 tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat
    12061 cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac
    12121 cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc
    12181 aatcaccacc agaccagcca gccccatcgc cacctgtcgc ccggtcttgc catgttgatg
    12241 tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg agcgcttgcc
    12301 acctggcgga ataattccct gtgggccgcc gtcgacgtca cccggtactt ccagcccgtg
    12361 ccagtgcaac gtcgtctctt ccgccagttg gttgtagata tcaaccgtta ccgctttgcc
    12421 gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg tagttgcagt
    12481 tttctcgcca aaggtggact gacctgcgcc aatagttaac tgaatgcgat tacgggcatc
    12541 ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga ctgcacggct
    12601 ccacagtggc aaggctgaag ccacacccag tgcgacggaa tatttcaaga aatcacgacg
    12661 ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc
    12721 cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc
    12781 ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc aatgaagacg
    12841 tttttcagaa cagtgttatt cggcagcctg atcgccgttt gcgcaaacag ttacgcgctc
    12901 agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt tctgaagaac
    12961 gattgtggtt accagaactt atctaacggg caaattcgtc gcgcactggt ctttttcgcc
    13021 cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc tctcggtgaa
    13081 gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaaatg caaagccctg
    13141 gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc tttttgtaga
    13201 aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt tttttacggg
    13261 tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga aacgctacac
    13321 gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa gaccgatcac
    13381 caggatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct ggatggcgta
    13441 gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca tgttccgcta
    13501 ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg tgccatgctg
    13561 cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat cgatgcgggt
    13621 gtcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta cgacgatccg
    13681 cgctttcaac tagtgatcga cgatggcgtc aatttcgtta atcaaaccag ccagaccttt
    13741 gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagcct gttcacctca
    13801 gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt cgcacaaaac
    13861 ggcgtctgct ttttacagca ggaagaagcc atcgacagcc atcgcaaact cagccattac
    13921 ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg tatcatgact
    13981 tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat tattcaggcg
    14041 cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca tacggcagct
    14101 tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta aggagaagat
    14161 aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag tctgagtttt
    14221 tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta tattgcttat
    14281 atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac ctgttccatt
    14341 atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg tgccagcgtc
    14401 actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac agaacacccc
    14461 ggcccactgc cagaaacggt ggttgctcat ctcgataaaa gtcatatttg cgtacatacc
    14521 tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat tgaagtctct
    14581 acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca gcttgagtcc
    14641 gatatcgtaa ccattgatta tcgcgtgcgc ggttttactc gcgacattaa cggtatgaag
    14701 cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga tatgaaggcg
    14761 ctgtatgaca tggtggatgt aaacgtctat caggaaaata tcttccatac caagatgttg
    14821 cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga tttaaccgac
    14881 agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg
    14941 cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc agatttattt
    15001 acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc tcaacgccgc
    15061 atagcgacag gctttcttcg tcgtagtagt tcatcccctc ttccatttca tcgccagcga
    15121 attccagttg attggcgcga accatcacct cttcagcgtc catccacagg gtgtattcat
    15181 gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct tgttccactt
    15241 catcaagcaa ggccaggttt tctttcacct cttcattaaa ccagtgcccg accacttcat
    15301 gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat tcgtaatcca
    15361 tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca tcgcgccatt gatgtaatta
    15421 tcgcagcctt accggagaaa aacagcggaa cggcgatagt ttagaaataa aaaatgcccg
    15481 ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt acttattaca
    15541 ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt
    15601 tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact
    15661 cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca
    15721 ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag gtggaaacca
    15781 ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag cccgggatct
    15841 cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc agttgcgcgg
    15901 cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct ttgtgcgcat
    15961 ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa ccgataaata
    16021 cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc ggatcgttcg
    16081 gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact gccgcgtatt
    16141 ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa
    16201 tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac ttcagcagga
    16261 cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag
    16321 cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact ttcagatccg
    16381 gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa
    16441 cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga tcgcgcaggg
    16501 tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg
    16561 gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg aaagagatac
    16621 cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac
    16681 ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg ccacggttca
    16741 taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg
    16801 tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca
    16861 gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt
    16921 cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg
    16981 ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt
    17041 gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata atcagcggaa
    17101 tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca
    17161 gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt
    17221 cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg tcttccatcg
    17281 tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg
    17341 gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg
    17401 atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag
    17461 cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata ccttcacggg
    17521 cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg
    17581 ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta acggtcagtt
    17641 tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct
    17701 gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca
    17761 tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg
    17821 cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatagctt
    17881 tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc aggaagcctg
    17941 cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca
    18001 ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg gccatttggt
    18061 ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt
    18121 attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct
    18181 tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat
    18241 agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga
    18301 atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt
    18361 gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac cgcctcttta
    18421 tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat
    18481 ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc
    18541 cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg aagcgcctgt
    18601 agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc ttcctgcaac
    18661 ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca ctgcacaaat
    18721 agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc aggtgctgat
    18781 ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg ataatccact
    18841 taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa
    18901 atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca
    18961 aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt
    19021 gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac
    19081 cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca taccatccaa
    19141 taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca tttccattga
    19201 gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa
    19261 ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttgt
    19321 tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt acgcactatt
    19381 cagcagcatc gactgagatg acgacgatca tgaccatcat catcatgacg atgatgatta
    19441 tcattaccac gatggcggtc atcgtgacag taatggctgg caacacaacg gcgacaacat
    19501 caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat
    19561 ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc
    19621 aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc
    19681 aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa cacacgccgc
    19741 ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga
    19801 acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca
    19861 gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc gccaggcagt
    19921 ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcgg cgcgccagcg
    19981 tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc
    20041 gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca
    20101 gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa
    20161 cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc gggagaaagt
    20221 tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga
    20281 gcaacggtca gcagtacgcg aacgtctctc tgaacgtgga gcaagaagac tggaaaggta
    20341 aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg
    20401 caaacgaaaa attacttctt cttcgctttc gggttcggca ggtcggtaat gctaccttcg
    20461 aacacttctg ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt
    20521 gcgatgtctt cagcatcaca acccatttcg attgccaggc cgatttcacc cagcagctcg
    20581 ccgccgttgg taccaacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc
    20641 agcttggtca taccgtctgc gcagtcagaa gcgatagcac gaccagaagc agcccacggg
    20701 aaggtggcgg tttcatagct gatgcctttc tctttcgctt ctttctcagt cagacctacc
    20761 catgcaactt ctggttcggt ataggcgatg gacgggataa ctttcggatc gaagtagtgt
    20821 ttcttaccgg cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc
    20881 atcggctgac cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt
    20941 ttgtcaacgc ggatgaaacc acggtcgtca acttccacgc cagctttgcc tgcgtcgagg
    21001 tttttaccgt tcggcacacg accaatcgct accagcacgg cgtcgtaacg ctgcggttca
    21061 gctggtgctt ttttgccttc catcgtcaca taaataccgt cttctttcgc ttcaacggcg
    21121 gtaactttgg tttccagcat caggttgaat ttcttgctga tacgcttggt gaagacttta
    21181 acgatgtctt tgtcagctgc cgggataacc tggtcgaaca tttcaaccac gtcaatctgt
    21241 gaacccagcg cgtggtaaac ggtgcccatt tccagaccga tgataccgcc acccattacc
    21301 agcaggcgtt ctggtacttc tttcagttcc agcgcgtcag tggagtccca gatacgcgga
    21361 tcttcatgcg gaataaacgg cagttggatc gggcgagaac ccgctgcaat gatcgcattg
    21421 tcgaagttga tcacggtttt gccgttctca ccttcaactt ccagggtgtt agccccggtg
    21481 aatttaccca gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca
    21541 ccggtcagct gattgatcac tttctctttc caggtacgaa tcttgtcaat atcggttttc
    21601 ggttcgccga agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct
    21661 acgtgcagca gtgctttaga agggatacag ccgacgttca gacaaacacc gccaagggtg
    21721 ttgtaacgtt ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag
    21781 taacctgcgg ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg
    21841 acctctatat atttatctcc ggcggtcata cccgtcgtct ttcaggccgc agcggcgtta
    21901 gcttcacgcc ctcaccccag tcacttactt atgtaagctc ctggggattc tggcgcttgc
    21961 taccttgctg cacccagaaa tccatagggt atgtgaagta ataccctaac caccaccggg
    22021 tcgttctatc cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac aaacggcaac
    22081 cgatttgtct attcgctaat aacctcaata cattcatgag attaccagaa aaaagccggc
    22141 cgttgggccg gctcttttac ttacatcacc agacggcgaa tgtcagacag cgtgttgtta
    22201 atgatggtaa tgaaacgggc accatcagca ccgtcgatca cgcggtggtc gaaggagaga
    22261 gaaatcggca gcatcagacg cggcacgaac tctttaccat tccacaccgg ctccatcgcg
    22321 gacttggaaa cgccgaggat agccacttcc ggcgcgttca caatcggcgc gaagtgggta
    22381 gtacccaggc cgccgatgct ggagatggtg aagcaaccgc cctgcatttc gcccgcagtc
    22441 agcttaccgt cacgcgcttt cttagaaata gtcatcagct cgcgagacag ctcgatgatg
    22501 cctttcttgt tgacgtcttt gaataccgga acaaccagac cgttcggggt atccaccgcc
    22561 acaccgatgt tgatgtattt cttcagggtc agacgctgac cgtcttccga cagcgaacta
    22621 ttgaagcgag gcatctgctc aagagctgca gcaacggctt tcatgatgaa gacaaccggg
    22681 gtgatcttca catccagctt acgtttcgcc gcttcttcgt tctgctgttt acggaacgct
    22741 tccagctcgg tgatatcggt tttgtcgaag tgggtaacat gcgggatcat cacccagtta
    22801 cggctcaggt tagcaccaga aattttctgg atacggccca gttccacttc ttcgatttcg
    22861 ccaaacttgc tgaagtccac cttcggccac ggcagcatgc cagggatacc accgccagtc
    22921 gccgccggag ctgcttctgc acgtttgata gcttctttca cgtaagcctg aacgtcttcg
    22981 cgcaggatac gacctttacg gccagtgccc ttcactttcg ccaggttaac gccaaactcg
    23041 cgtgccagac ggcggatcag cggggtcgcg tgaacgtaag cgtcgttttc agcaaattca
    23101 gatttgcctt ccgctttcgc agccggagct tcagcttttg ctgccggagc cggcgctgcc
    23161 gcttcctgtt tcgcaggagc tgccgcaggc gctgcgcctt caacttcgaa gatcataatc
    23221 agcgaaccag ttttcacttt atcgccaacg ttgactttca gttccttcac gacgcctgca
    23281 aacggagccg gaacttccat agaagcttta tcgccttcta cggtaatcag tgactgttca
    23341 gcggcaactt tgtcgcccac tttcaccatc acttcggtca cttcaacttc gtcaccgccg
    23401 atatccggaa cgttaacttc tttcacgcca gctgctggtg caggggtcgc tgccggagtc
    23461 gcttcctgtt tagccgccgg agctgccgcg cctgcttcac ccgcgacttc gaagaccata
    23521 atcagcgagc cggtagacac tttgtcaccc acgttcactt tgatctcttt cacggtacct
    23581 gcaaacggag ccggaacttc catagaagcc ttgtcgcctt ctacggtgat cagcgactgt
    23641 tcagcttcaa ctttatcgcc aactttcacc aggatttcgg tcacttcaac ttcgtcgctg
    23701 ccgatatccg gaacgttaac gtcttttgcc gccgcagccg cgggtgctgc tgccggagct
    23761 gcttctttct tctcttctgc ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg
    23821 aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt gatctcttta
    23881 acgatacccg cctgcggaga tggaacttcc atagaggctt tgtcgccttc tacggtgatc
    23941 agcgactgtt cggcttcaac tttgtcgccc actttgacca ggatctcggt gatttcaact
    24001 tcatcagccc cgatgtccgg tactttgatt tcgatagcca ttattctttt acctcttacg
    24061 ccagacgcgg gttaacttta tctgcatcga tgttgaattt ggcgattgcg tcagcaacca
    24121 ctttcttatc gatttcgcca cgtttagcca gttcgcccag cgccgcaacc acgacataag
    24181 aagcatcaac ttcgaagtgg tgacgcaggt tctcacggct gtcggaacga ccgaagccat
    24241 cagtacccag tacgcgatag tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca
    24301 gtttcatata gtcggtagat gccactgccg gcgcgtcgtt catcacctga gcgatatacg
    24361 gaacgcgcgg agtttccagc ggatgcagca tgttccagcg ttcacaatcc tgaccatcac
    24421 gcgccagctc ggtgaaggag gtcacgctat aaacgtcaga acctacgccg taatctttcg
    24481 ccaggatctc tgccgcttca cggacgtgac gcaggatgga accggagccc agcagctgaa
    24541 ctttaccttt gctaccttca atggtttcga gtttgtagat acctttacgg ataccttcct
    24601 cagcaccttc cggcattgcc ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt
    24661 aaacgttctc ttgtttttca ccgtacatac gctccagacc gtcatgcata atgacagcaa
    24721 cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg gatagtcagc gactgaatgt
    24781 ggctgtgacc atcttcgtgc tgcagacctt caccgttcag ggtggtacga ccggaagtac
    24841 cgccgatcag gaagccacgc gcttgctggt ctccagccgc ccagcacaga tcgccgatac
    24901 gctggaagcc gaacatcgag taatagatgt agaatgggat catcggcaga ttgttcgtgc
    24961 tgtaagaggt cgccgctgcc agccaggaac accctgcgcc cagctcgttg atcccttcct
    25021 gcagaatctg acctttctcg tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg
    25081 tgtactgctg accgttcggg ctgtaaatac caatctgacg gaacagacct tccataccga
    25141 aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc tttgatcgac ttgttcttca
    25201 gcatcacgtt cagagcacga acgaaagcga tagtggtaga gagctctttg ctctgctctt
    25261 ccagcagcgc gccgaagtct tgcaggctcg gcagctcaag cttctcggtg aagttcggct
    25321 gacggcttgg cagataaccg tgcagtttct gacgctgagc gtgcagatag gtatgctctt
    25381 cagaaccttc cgggaaggtg atgtacggca gtttttcgat atctgcatca gacaccggca
    25441 cattgaaacg gtcgcggatg tgacgcacgc cgtccatgtt cattttctta acctggtgcg
    25501 cgatgttttt accttcagcc gcgtcgccca tgccgtaacc tttaatggta tgagcaagga
    25561 ttactgtcgc tttgcctttg gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg
    25621 gatcgtgacc accacggttc agtgcccaga tctgctcgtc agtccagtct gcaaccagtg
    25681 ctgcggtttc aggatattta ccgaagaagt gttcacgaac gtacgcacca tctttcgatt
    25741 tgaaggtctg gtagtcgccg tcaacggttt cgttcatcag ctggatcagt ttaccgctgg
    25801 tatctttacg cagcagttca tcccaacggc taccccacat cactttgatc acgttccagc
    25861 cagcaccttc gaagatgcct tccagttcgt tgatgatctt gccgttaccg gtgaccgggc
    25921 cgtcaagacg ctgcaggtta cagttgataa cgaaaaccag gttatccagt ttttcacggg
    25981 tagcgatggt gatcgcacct ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg
    26041 cgtaaacggt ctgtttagag gtatctttca ggccacggtg ttccagatat ttcaggaatt
    26101 tagcctggta aatagcacca atcggaccca gacccataga tacggtcggg aactgccaga
    26161 attccggcat cagtttcggg tgcggatagg aagagaggcc attgccgtga acttcctgac
    26221 ggaagttatc cagctgctcc tgagtcagac gaccttccag gaaagcacgg gcgtaaacgc
    26281 ccggggagat gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac
    26341 ggaagaagtg gttaaagcac acatcataaa tggttgcgga agactggaag gacgccatgt
    26401 ggccgcccag ttcgaggtct tttttcgacg cacgcagcac cgtcatgatg gcgttccagc
    26461 ggatagctga acgaatacgg cgttccagtt ccagattacc cggatactcc ggttgttctt
    26521 caacggggat ggtgttgatg tagttgctga tacctgtgcc tgcggctacg tttacaccgc
    26581 ctttgcgggc ttcagcaagc agttggtcga tcagatactg agcacgctca acaccttctt
    26641 cacggatgac cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat
    26701 ttgggaaacg ttctgacatg ggttattcct tatctatcta ataacgttga gttttctgga
    26761 acctgtccca ttgaactctc gccagaaagc tcaataagac aggttctacg tttagttacc
    26821 gcgcttttat atgcgcttga tttacaacat cttctggata atttttacca gaaaaatcac
    26881 taattctttc gttgctccag acgacgcaga gaacgctcac ggcggctctc ttcacggctt
    26941 ctgtcgagca aaatttcttc gataaaggcc agatggcgat gcgatgcttc gcgcgcttct
    27001 tccggcttac cgaccataat cgcttcaaat atgcgggtgc ggtgactact caccagcggc
    27061 agcatctcgc gacgcgaata gagcaattcg aagttctggc ggacattctg ggccaacatc
    27121 ggctccatac accttagcag atgaagcaga accacattgt gggccgcttc ggtgacggca
    27181 atctgatact ggagtacggc gtttgattcc gcgtccagat cgccagactg ctgcgccagc
    27241 tctatggcgt ggtggagttc acggatgcgt tccttgtctt catcggtact acgcagcgcg
    27301 gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc atactgtgat
    27361 tcaggatggt cggagagcag ctccaccagc ggatcgctga agctttgcca taggctgctc
    27421 tggacaaaag tgccgccacc ctggcgacga agcaacaagc ccttcgcttc gagacgttga
    27481 atcgcctcac gcaaggaggg acgggagacg tcgaactgtt ttgccagttc gcgttccggt
    27541 gggagttttt cgcccgggcg gagagtgcct tcgaggatca aaaactccag ttgctgctca
    27601 atcacatcgg agagttttgg ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag
    27661 ccacttgccg aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag
    27721 tattcacctt atgtccatac aggttttgat tgaaatcatg aaactgtgca cattttaaca
    27781 acttgacata tataacgttt caaagttgta actatgcaca aatgtagact ttacgtagga
    27841 aagggatttt ttaacgatta ttaaactata aaaatccgaa ttgaaccgat tcacttacca
    27901 attttttgat ttttaagtca ataaaaacga atttaaattc actctgcata ttgagagggg
    27961 ttgaggctga gctttacaaa cggtttcttt ttaagcaact catcttcaac catgcataaa
    28021 gcgggtgcat tcgctgccgc ataccattat tcttgatctg acggaagtct ttttgtaaca
    28081 attcaaacct ccttgatgta aacaaatcaa tacaacaaac ggaattgcaa acttacacac
    28141 gcatcactgc gtagatcaaa aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac
    28201 agcacggcga gcagctaaag cgcggcctta aaaaccgcca tattcagctt atcgcgctgg
    28261 gtggcgcgat agggacaggg ctattcctag gtagcgcctc cgtaatacag tccgcagggc
    28321 cagggattat cctgggttac gccattgctg gttttatcgc ctttctgatc atgcgtcagc
    28381 tgggtgaaat ggtggtcgaa gaacctgtcg caggctcctt tagccacttt gcttataaat
    28441 actggggcag ttttgccggt ttcgcctctg gctggaacta ctgggtactg tacgttttag
    28501 ttgccatggc agagctgact gccgtgggta aatacattca gttctggtat ccggaaatcc
    28561 caacctgggt ttctgccgcc gtattctttg tggtgattaa cgccatcaac ctgaccaacg
    28621 ttaaagtgtt tggcgagatg gagttctggt ttgccattat caaagttatt gcggtagtag
    28681 cgatgatcat cttcggcggc tggctgctgt tcagtggcaa cggcggcccg caggcgagcg
    28741 ttagcaacct gtgggatcaa ggtggtttcc tgccgcacgg cttcaccgga ctggtgatga
    28801 tgatggcgat tatcatgttc tcgttcggtg gtctggaact ggtggggatc accgcagcag
    28861 aagctgataa cccggagcaa agtatcccga aagcaactaa ccaggttatc taccgcatcc
    28921 tgattttcta tattggttcg ttagccgttc tgctctcact gatgccgtgg acccgcgtta
    28981 ccgccgatac cagtccgttt gtgctgatct tccacgagtt aggcgatacc tttgtggcga
    29041 atgcgctgaa catcgtggta ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt
    29101 gcaacagccg tatgctgttt ggtctggcac aacaggggaa cgcgccaaaa gcgctggcgt
    29161 ctgtcgataa acgcggcgta ccggttaaca ccattctggt gtctgcgctg gttacagcat
    29221 tgtgcgtatt gattaactat cttgctccgg aatccgcatt tggcctgtta atggcactgg
    29281 tggtatccgc actggtgatc aactgggcga tgatcagtct ggcgcatatg aagttccgtc
    29341 gcgccaagca ggaacaaggc gtggtaactc gcttccctgc tctgctttat ccgctgggta
    29401 actggatctg cctgctgttt atggcggcgg tactggtgat tatgctgatg accccaggaa
    29461 tggcgatttc ggtatacctg atcccggtat ggctgatcgt gttaggtatc ggctatctgt
    29521 ttaaagagaa aaccgccaaa gccgtaaaag cgcattaatc tctctacgcc ctcacccgta
    29581 cagggtgagg gcaataatct ctacaccaac gccccgtaaa tcgtcagtag cgcaatcacc
    29641 accacgacca cgaacgaggt tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc
    29701 ttatcgacat gcggttcacg cgccagagag aactgcgcca gacgcgttaa tacctgatat
    29761 tgcgaagtat ggaaatcacc cagcgaagca aaccaggccg gtaacgcttt ctcaccatga
    29821 ccgatcaagg catataccac acccgcaaga cgaaccggca cccaatccag tacatgaagc
    29881 actgcatcaa tgccggactg taaacgatga tgcggcgtct ggtatcgcgc cagccagtat
    29941 tgccatgcac gcaagaacgc ataccccatc agcgtaacgg gtccccaggt tccccccaca
    30001 atcagccaga acagcggtgc aagataaaaa cgaaagttaa tccacagcaa tgcattttgc
    30061 agctcacgca aatactcacg ttcgtcgcag cctgccggga cgccgtgaat catggtgagt
    30121 tcgccagcca tcgtggcacg ggcatggcta tcattacgtg aagcagctgt cagataagca
    30181 tgataatgaa gacgaacttt acctgcgcca atacacagca aaccaatcag cagccacacc
    30241 agcagcgtgg gaacgttgaa caatactccc tgcaatgcgc gtaacagtaa aaaagtcaca
    30301 cccatcgcaa taatggtcat gcctaacgtg cgcccgagag aaaaatgttt cacccgccga
    30361 aagaacgctt caagacgatg atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg
    30421 aaaattaaca ccagtaaggt tgtaaatagc gtcatgttgt ctccttgctg accagcgcac
    30481 gaaaccgtgc ccaatcaaat gcaggaccgg gatcggtttt ccgatccggc gcaatatcac
    30541 aatggcccgt catgttatta gcgatatcgg gatagcgatc tatcagtgcc cgcgtaaccg
    30601 ccgcaagctg ttgatactgc gcatcggtat acgccagagt atcggtgcct tcaagctcaa
    30661 tcccaataga aaaatcattg cagcgttcac gcccctgata ctgagagact cccgcatgcc
    30721 aggcacgttt atcgaaagga acatactgga ctatttcacc atcacggcga atcaaacagt
    30781 gagcggagac gcgcaaatgg gcgatctcag caaagaaagg atgtgcctgc ggatcaatag
    30841 ttccagtgaa taatgcgtcg atccacggac cgccaaactc gcctggcggc aggctaatat
    30901 tgtgcaccac cagcagggtg ggtgtttcgt catccaggcg gcaatcgtaa tgtggtgagg
    30961 gaacgcggcg cgcgccaacc agccacccct gttctaacaa catgcaggat ctccttatat
    31021 gtggtgctaa tacccggttc agagtagcat gtttctacct tatgattcgt tagctatctg
    31081 gagttttaac atgccgcctc gccgctacaa ccctgacacc cgacgtgacg agctgctgga
    31141 acgcattaat ctcgatatcc ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg
    31201 aacagtcgat gccaacaatg acattacggc aaaactttta ccggaaaatt ctcgctctca
    31261 tgccacggtg atcacccgcg agaatggcgt attttgtggc aaacgctggg ttgaagaggt
    31321 gtttattcaa ctggcaggcg acgatgtcac cataatctgg catgtggatg acggcgatgt
    31381 catcaacgcc aatcaaccct tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg
    31441 cgaacgcact gcgcttaatt ttgtgcaaac cctttcagga gttgccagta aggtacgcca
    31501 ctatgtcgaa ttactggaag gcaccaacac gcagttgttg gatacgcgca aaaccttacc
    31561 tggcctgcgt tcagctctga aatacgcggt actttgcggc ggcggagcga atcaccgtct
    31621 ggggctttct gatgccttcc tgatcaaaga aaaccatatt attgtctccg gctcagtgcg
    31681 ccaggcggtc gaaaaagcgt cctggctgca tccggatgcg ccagtagaag tcgaagtaga
    31741 gaatctggaa gaacttgatg aggccctgaa agccggagcc gatatcatca tgctggataa
    31801 cttcgaaaca gaacagatgc gcgaagccgt caaacgcacc aacggcaagg cgctactgga
    31861 agtgtctggc aacgtcactg acaaaacact gcgcgaattt gccgaaacgg gcgtggactt
    31921 tatctccgtc ggtgcgctaa ctaaacacgt acaagcactc gacctttcaa tgcgttttcg
    31981 ctaattgctg acagatttat ccgctccatc gcgggcggat aatctcccct tccccaactt
    32041 tcttcgtaac gcctcgcaaa tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa
    32101 tctgctttcc cgtttcgttt tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc
    32161 accaaccaaa tcaaggagcg aaacagatgg acaagcaacg cggttttaca cttatcgaac
    32221 tgatggtggt tattggcatc attgccattt taagctccat tggtattccc gcttatcaaa
    32281 actacctgcg caaagccgca ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg
    32341 ccgtagagtt gtgcgcgctg gaacatggtg gattagatac ctgcgacggt ggcagcaatg
    32401 gcattccctc gcctaccacc acccgctatg tttcagccat gagtgtggca aagggcgtgg
    32461 tgtcgctgac cgggcaagaa agtctcaatg ggctaagcgt cgtcatgacg ccaggttggg
    32521 ataacgcaaa cggcgtcacc ggctgggcgc gcaactgcaa tattcaaagt gacagcgcat
    32581 tgcagcaagc ctgcgaagat gtcttccgct ttgatgacgc caactaagga gcggcaatga
    32641 atattccaca gctcacggcc ctgtgcctgc gttatcaggg agtcttgctg gatgccagcg
    32701 aagaagtggt tcatgttgcg gtggtcgatg ccccctcaca tgagttgctg gacgcattgc
    32761 atttcgctac caccaaacgt attgagatca cctgctggac gcgccaacaa atggaaggtc
    32821 acgccagtcg cacacaacag acattacccg tagctgttca ggagaagcat cagcccaaag
    32881 cagagttgct gactcgaacg ttacaatctg cgctggaaca acgcgcgtct gatattcata
    32941 tcgaaccagc ggacaatgcc taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt
    33001 taccggatgt ttcaccggat gccggagtcg cattaacagc cagattaaaa gtgctgggaa
    33061 acctggatat tgcggaacat cgcctgccgc aggacgggca attcactgtc gaactggcag
    33121 gaaacaccgt ctcatttcgt attgcgacct taccatgtcg gggtggtgaa aaggtggtat
    33181 taaggttgtt acagcaggtg agccaggcac tggatgttaa cacgctggga atgcagccgt
    33241 tacaactggc ggactttgct catgccttgc aacaaccaca gggactggtg ctggtaactg
    33301 gccctacagg cagtggcaaa acggtcacct ggtgttgtcc acgctacaca ccaattccac
    33361 cactgaaacg ctggtacgtt tacagcaaat gggggtcgcc cgctggatgc tatcatcggc
    33421 gcttacgctg gtaatagccc agcgtctggt acgtaaactt tgcccacatt gtcgccgaca
    33481 gcaaggggag cccatccata ttccagacac tgtatggccg tcgccgctgc cccactggca
    33541 ggcacccggt tgtgtacatt gctaccacgg tttttatggt cgtaccgcct tgtttgaagt
    33601 cctgcccatt acaccagcca tacgccagct tatttccgct aatactgaag ttgaatcgct
    33661 ggaaacgcac gcccgacagg cgggtatgcg tacgcttttt gaaaacggct gcctggccgt
    33721 ggagcaaggc ttaaccacct ttgaagagtt aatccgcgta ctggggatgc cgcatggcaa
    33781 gtaagcaact ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc
    33841 tatgggcaga gagccgtact ttactgctta tggcactaca gcaacagatg gttaccccac
    33901 taagcctgaa gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag
    33961 tcattcatca actggcgacg ctacttaaag ccgggttaac gctttctgaa gggctggctc
    34021 tgctggcgga acagcatccc agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg
    34081 atctcgaaca gggcattgct ttttccaatg ccttattacc ctggtcagag gcatttccgc
    34141 cgctctatca ggcgatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct
    34201 ttgaactggc gcgtcagcaa aaatcccagc gtcagttgac cgacaaagtg aaatcagcgt
    34261 tgcgttatcc catcatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt
    34321 ttgttctgcc ggagtttgcc gctatctata agaccttcaa caccccacta ccggcactaa
    34381 cgcaggggat catgacgctg gcagacttta gtggcgaatg gggctggctg ctggtgttgt
    34441 tcggctttct gctggcgata gccaataagt tgctgatgcg ccgcccgacc tggcttatcg
    34501 cgcggcagaa attgctgtta cgcatcccga ttatgggttc actgatgagg ggacaaaaac
    34561 tcacgcagat ctttacgatt ctggcgctga cacaaagtgc aggcattact tttttacagg
    34621 gcgtagagag cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa
    34681 tccagcacga tatcagtaac ggtcatccca tctggctggc gctaaaaaat gccggtgagt
    34741 ttagcccgct ctgtttgcaa ttagtgagaa caggagaggc atccggctcg ctggacctca
    34801 tgttagacaa cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg
    34861 cagccttact ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg
    34921 tggcaatgta tctgccaatt ttccatttag gcgatgcgat gagtgggatg ggataacgct
    34981 ggcgtgttgc tccacgccag cgttgggaga ttacaggttg ttgaagatgc ggttttcttg
    35041 ttccagcaca cgaataaacg tggtgcgctt ggtcagctct ttcaggcgtg aagccccaac
    35101 gtatgtacaa gctgaacgca ggccgcccaa aatatcacgc gcggtatttt caacctggcc
    35161 tcgcagcggc agcttaacgg ttttaccttc tgctgcgcgg tattccgcaa cgccgccaac
    35221 gtgacgtttc atcgcagact cggagctcat gccgtagaac agcataaatt tctcgccgtt
    35281 ctcctcaacg atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcatgac
    35341 gaaatcggca ccgccgccaa aggctttcgc cacatcgccc ggcgtggtac agccaccgtc
    35401 gctgacgatc attccgccca gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag
    35461 ctgcggataa ccgacgcctg ttttgacgcg agttgtacaa acagaacctg ggccaatgcc
    35521 aactttaacg atatcagcac cggagaggat aagctcctca cacatttcac cagtcactac
    35581 gttgccagca caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac
    35641 gaagtgttcg gaataaccat tcgccacatc aatacaaacg aagtttaatg ccgggttcag
    35701 gtcgagaatc tgtttagttt tttcgaaatc cgcatcagac gtgccggtag aaaccatcac
    35761 atgtttcagc acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaaaa
    35821 gtgtttatgc acagcagtca gaatatcaaa agaagccagc gcagaggcca tagaaaatgt
    35881 gcctacggtg tccatatttg cggcgataat cggcacgccg gaccagctct gacctgaatg
    35941 tttgaaggtg aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt
    36001 agggcggatg agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg
    36061 attcctgggg ttaatggcta taagggtaag agcgcaactc cagtgacgct atcatacgca
    36121 ctaatcaatg cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt
    36181 tacctggtaa atagtgagcg aacgtttcat caatttcact ttcgcgcgct catttttaac
    36241 gttttgataa tcatgatgaa ttcccgggaa tataaattat ggggtatata gttgccttaa
    36301 cgggaggcat tggcagtggt aagagtaccg ttgccaatgc gtttgctgat ctcggaatta
    36361 acgtcattga tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac
    36421 atgccattgc tgatcacttt ggcgaaaaca tgattgctgc tgatggaaca ttgcagcgcc
    36481 gggccttgcg cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgacctgc
    36541 tgcatccgct gattcaacaa gagacgcaac accagatcca gcaagcaact tccccctatg
    36601 tactgtgggt tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc
    36661 tggtggtgga tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa
    36721 ctcgcgaaca tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg
    36781 tggcagatga cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc
    36841 gcctgcacgc acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat
    36901 gcagacccag gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat
    36961 tgagtttttg attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct
    37021 gcatttcttc cgtaatgtca gtgaattact ggatgttttc gagcgcggcg aagtccgcac
    37081 tgagctgttg aaagaactta accggcagca acgtaaactc cagacctgga ttggcgtgcc
    37141 tggcgtggac cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggtgagcgt
    37201 attaatttcc gcgccgcgta tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt
    37261 gcgtcagcga ctgagcatcc caggcggctg ttgcagcttt gatttaccta cattgcacat
    37321 ttggctgcat ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct
    37381 gaacccgctc acccaggcac ttaccatggt gctagattta attcgccagt cggccccctt
    37441 ccgtaaacaa accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct
    37501 gcgcctgaat ctgtcgctcg attcacagct ttatccgcaa atttccggac ataagagccg
    37561 ttttgccatt cgttttatgc cgctggacac tgaaaacgga caggtaccgg aacgtctgga
    37621 tttcgaactg gcctgttgct aaggagtaaa aatgtcagaa actattacgg tgaattgccc
    37681 aacctgcggg aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa
    37741 acgttgtcag ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag
    37801 tggcgatctt tccgaaagcg atgactggag cgaagagcca aagcagtgac atttgtctga
    37861 tgcctcacgt aggcctgata agacgcggac agcgtcgcat caggcatctt gtgcaaatgt
    37921 cggatgcggc gtgaacgcct tatccgacct acagacgctt aagcttcgca attaccggtt
    37981 cattggctgg cggaaaatca tcggcattaa gaccgaccag cgacatccac tcaccgggtt
    38041 gcccttcttt accccacggc cccccttccc aactttcgac cagccagaac cacagagtta
    38101 tatgcctgtc cgggaattca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa
    38161 tcccgacctc ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg cccatttcaa
    38221 ttttaccgcc gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgtgtta
    38281 taaagatttc attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg
    38341 tcttaaacct ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta
    38401 ttgcaggcga ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt
    38461 acgtcctact ttgcgttcac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc
    38521 atcctgatgg ctaagctgct gcatttgtgc taaacgctcg gcttccatac gacgctgttg
    38581 ttccagctcc tcaacctctt caggcatacg tacctgaact ttgctcagcg tactgataac
    38641 ttcatatttc aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc
    38701 ctgcttcgga tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat
    38761 cgctgccagg tgctctttcc acagggagtc aagcgtttgc agcatgacgc ctttctcgaa
    38821 gtgacgcatc atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg
    38881 cgccagaatg cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc
    38941 ggtaattggt aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc
    39001 ccacatttct tccagcgact gtggcggaat gtaggcatca atggtggctt tgaacacatc
    39061 ttcacgaatg ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg
    39121 ggagtaaatg gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg
    39181 aatgtcgaag ttacgacttt caactttacg ctgggcgttg gcaatcgctt tagtcaccca
    39241 cgggtgttca atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg
    39301 gtcggaagca aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc
    39361 agcatccccc tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg
    39421 acgctcggta ccgatgatat gcaggccacc tgcttccagt accgcatcgt gacgtacctg
    39481 ccagtcggct ttaatttttt caatttgctc tgcggtcgga ttttccagcg cggcaacttc
    39541 tgcctgccag ctacctccga gcacaatatc tgtaccacga cccgccatgt tggtcgcgat
    39601 agtcaccgca gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt
    39661 ggcgttcagg acattgtgct taataccggc tttggtcagt tcgtttgaca ccagctccga
    39721 tttttcgatg gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat
    39781 atcttcaatg atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt ccggcagatc
    39841 tttacgaatc attggacggt tggtcggaac aacgacggta tccagtttgt agatggagct
    39901 aaattcgaaa gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg
    39961 gaagtagttc tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc
    40021 ttctttcgct tccacagcct ggtgcagacc atcggaccag cgtcggccct gcatggtacg
    40081 accggtgtgt tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcacg
    40141 ggtaaacagc gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc
    40201 cggagagtac agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat
    40261 cagcaccaga ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg
    40321 gccttcgccc tggaaggttt cggagtcttc tttttcctga cggatcaggt gcggaataat
    40381 tttattcacg cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg
    40441 tgtacgcgct tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt
    40501 acgctgtaca cgttcttcag ggctgaacgc catgttgtcg cgcaggtagt caaagccgta
    40561 ttcgttgttc gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg ccggtgctgg
    40621 catgcccggc aggttgatac caacagtcag gccaaggaat tcaaacagcg gacggttgtt
    40681 ttcggcgtca cgttgcgcca ggtagtcgtt gacggtaact acgtgcacgc ctttaccggt
    40741 tagtgcgttc aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat
    40801 ttcggcgatg cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg
    40861 cataccaaag acgcgcttac ttgcctcacg caccacggcg aaagcttccg ggatcagatt
    40921 ttccagcact tcgccttttt ccagacgcgc acgaaactct gcggttttcc ctttcagttc
    40981 ttcatcggag agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg
    41041 catccggcgc agggtgcgat cgttacgact accgaaaact ttagttaaca atttgattag
    41101 cataataaaa tctcaaacgc cccgcgttac ggagttaata aaatgaagta aaggtttatt
    41161 gttgttaggt gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg
    41221 gcgtgctgaa ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag
    41281 acggcgtgcc ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg
    41341 cctgaagagg caaagattct tcagcaacgg gcagtgtttg cggtgccatt gcgaaagaaa
    41401 gatgacggat taccgtacga atagcatgtt gatgccagta atcaacggaa tagttcgaat
    41461 tcgggcggcg cgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag
    41521 gctcgtggtt gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg
    41581 caggcaaacc taaactcgcc gcgaccatcc ctaataagag atgcggccag aagtagcgtt
    41641 taccaaactg tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgaatt
    41701 caaacgtatt tagcgcacaa atcttatcat taaagcctga agtccacagc acgaatgaag
    41761 ataagttgtg cgtaaaagtg cttaagaaaa cagcgttcgc accgtttttt atagctctta
    41821 taaaattcaa aaaaaaacga ctggttcacc tggccggaga gagtgccaga tttgccagtc
    41881 gaattttata cgacagtata aatgtcgtta tgccagtaca gctgaaggcg ctttgaaggc
    41941 caacggcagt tctgcgtcgt cctggaaggt cacatattcc caggcttcct gtttcgccag
    42001 gacagcctgc agcagtttgt tattcagtgc atgaccggat ttataagcgg taaatgcacc
    42061 aataatattg tgaccacaca tgaacaagtc accgatcgca tcgagcattt tgtgacgcac
    42121 aaattcgtct tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt caacaacgat
    42181 ggcacaatcg aagctgccgc ccaggcacaa accacgggac tgcagatatt cgatatcacg
    42241 catgaaaccg aacgtacgcg cacggctgat ctggcgcata aacgcatcag cggagaagtt
    42301 catcgcatag cgctggttgc tggaatcaat agccggatgg ttaaaatcga tggtgaaatc
    42361 cagcgaaaaa ccattgtacg gcttaaattc agcccactta tcgccatctt cgacacgaac
    42421 agtctctttg atgcgaacaa attttttggc gcagttcaac tcgtcgatac cggcgtcaag
    42481 cagcaggtat acaaacggag cggcgctgcc gtccatgatc gggatttccg gcgcgttaac
    42541 ttcgataaca atgttatcga tgcccaggcc cgcgagagca gcattgaggt gctctacggt
    42601 tgaaatccgt acatcatgct cgttgaccag acacgtacag agcatggtat cacgcacaga
    42661 tttggcatcg gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat agatgacccc
    42721 ggtgttggcc ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat gtaaaccgac
    42781 acccgtcgcc tgaacgatac gtttaagtgt cctttgtttg atcatcgtat tatctcgcca
    42841 aattacctat ccaaccgaag tgtactatac attcggcggg ccagtttagc acaaagagcc
    42901 tcgaaaccca aattccagtc aattcttaat cagcttgctt acgtaggaat gctgggatat
    42961 ccagataatc cggctctttc gcagtttgcg gcgcattgtc attcactact ttagcaaccg
    43021 gcttctgctc ctgggtcagc ggagccatcc catgctgctg gtagcgatcc atcactggct
    43081 gctgaacctg cttattggtc accagagtga tttcaggacg tttgtccatg ccgatacctg
    43141 tcgcaacaac ggttacgcgc agctcgtcat tcatatccgg gtcaagagaa gtaccgataa
    43201 ccacagtcgc gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt tcgaactcat
    43261 ccagacgcag gtcgaagccc gccgtgatgt taaccagcac gccgcgcgcg ccagacaggt
    43321 cgatatcttc cagcagcgga gaagagatag ccatttcagc agcttcttcc gcacggtctt
    43381 caccgctcgc cacgccagaa cccatcattg cgtagcccat ttcagacatt acggtgcgta
    43441 cgtctgcaaa gtccacgttc atcaaacccg gacgagtaat cagttcggcg ataccttgca
    43501 cagcgccttt cagtacatcg ttcgctgcgc caaacgcatc cagcagggag ataccgcggc
    43561 ccagaacttt cagcagtttg tcgttcggga tagtgatcag agagtccaca tgcttggaca
    43621 gttcagtgat cccctgctcg gcgaatgcca tacgcttctt gccttcaaag ttgaaaggct
    43681 tagtgacgac agcaacggtc aggataccca aatcttttgc cacttcagcg acgactggtg
    43741 ctgcacctgt accggtacca ccacccatac ccgcagcaat aaagaccatg tctgcacctt
    43801 ccagcgccgc acgcaatgca tcgcgatcct catcagccgc attgcggcca acttctggat
    43861 tagcgccagc gcccagtcct ttggtgatac cgctaccgat ttgaatcgtc tgtccaaccg
    43921 ctgttttacg cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca ccttcaatac
    43981 gctcgcgcac catgtgttca acagcattac cgccgccgcc gccgacgccg atgactttaa
    44041 tcaccgcgtc attggtaagt tccattggtt caaacatagt ttctctccga tttgtgcctg
    44101 tcgcctgagt ccgtaatcat cgtcggcctc ataaaaatta aaactctttt cgcagccaac
    44161 tattgagtcg cttgatccac gagccaactg atgctgtaac acgtttttct acttcagctt
    44221 caccgttaag atgtgactct ttcccatagt gaagcaatcc caccgccgtc gaataatacg
    44281 gctcctgagc ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata cgcacttgcg
    44341 tatgaaacac gcgctgagca caggctgcaa gaccttcgat ctgcgctgcg ccaccggtta
    44401 atacaatgcc tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt tcctgcaact
    44461 gcaatatctc ttcgttgacc aggttgagca actcggtata gcgcggctcg atcacctctg
    44521 ccagggtctg acgttgcaga ctccgcggcg gacgaccgcc tacgctcggc acttccacgc
    44581 tctcatcttt tccaacgatg gaacccagcg cacaaccgtg gcgaacttta atcgcttcgg
    44641 cgtcgcttgg cggcgtgcca aaggcgtaag cgatatcact ggtcacgaca ttgccagcat
    44701 aaggaattac ctttgtgtgg cgcaatgccc caccggtata aacggcgata tccattgtac
    44761 caccaccgat atcgacgacg cagacaccca gttcacgttc atcttccgtc aataccgaat
    44821 aacttgatgc cagtccggca aatatcagtt ggtcaacttt cagcccacaa cgttcaaccg
    44881 ctttgacgat gtttttcgcc atatcgttgt gacatgtgat caggtgcact tttgcctgca
    44941 tccgcacacc cgaaagtcct accggattct tgatcccttc ctgatagtca atcgcatact
    45001 cttgcgggat cacatgcagc acacgatgct catcgcgcac acgcaccgat ttcgcggtat
    45061 ggacgacgtt ttccacatct tcttgcgtca cttcttcttc agaaataggc accataccaa
    45121 tttcattctg gcagctgatg tgcttaccag aaagcgccag atataccgaa gagatctgac
    45181 aatctgccat caattctgcc tggtcaatgg cgcgttgtac gcacttgacc acggattcga
    45241 ggtcgttcac cccgccttta tccataccac gtgacgggca gctgcccacg ccaatgatat
    45301 tgaccatacc gtcgggcaga acttccccta ctaaagcggc aaccttcgcg gtaccaattt
    45361 ccagtcctac taccagtttt ctgtccgtcg ccttgatcat tgttgttctg cctgtgcctg
    45421 attttgttgc tgagtagatt cctctggcgg caagggcgcc cagcctactg ccgctccaga
    45481 gtcataacgc aaatcaacgt agctaatccg tttgccatcg gtttgcgcct gctgctgtaa
    45541 aaccggataa agttctacaa agcgaaccaa acgtttcatc gtatcgcccc ggccaagatt
    45601 gagcttaata tcgttattca gcgtcaactg ccaggaacgc cgcgcggtca tcgccgcttc
    45661 cttcagagta aatctgtcct ttgccagcat ctgccccatt tcgcgatagc cctgcaacac
    45721 ttcattagcg ctgccttccg ggccatacag cattggaagc acctgcttgc tggtgcgatc
    45781 tggcggcacg ctgaaggtgt ttccttccgc gtctaccatg tgttgatcat tccaccgcgc
    45841 aatcggcaca tattcaacca gatgaatctt caattcatca ggccactgct ttctgacgct
    45901 cacctgctta atccacggca ggcgttgttc tatttgcgtc tggatgatgt tgacatcctg
    45961 ggtcataaag gtacccggct cacccaatgc caggatcgac tgccggatat cgtcattacg
    46021 cgtgtaatgg cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt gcgcatcttc
    46081 catccagctc aacacgaccc agccgctcac caacactgtc gttaaaacgg tcagcaggaa
    46141 aaggatcccc gccagacgcg ttccattatt gcggcgagaa gaaacctctt cttcgctgtt
    46201 tcgcgtgttc agagcagcct gcgacatatt agtccgccag ttccagaatt cgtactacca
    46261 actgcgagaa gctcatacct gcctgacgtg ccgccatcgg caccaggctg tggctggtca
    46321 tacccggtga ggtattggct tccagcagat aaaactgtcc atcactgtcc agcataacgt
    46381 caatacgtcc ccatcctttg caacctaacg tcgtccatgc tttcagcact aatgcctgca
    46441 aattggcctc ttgtgacgct tccagacctg cggggcagaa atactgtgtc tcatcagaga
    46501 gatacttcgc ctcataatca tagaaggttc cggacggttg aatacgtatt gacggtaaaa
    46561 tttcttcacc gagtatcgca accgtgaact ccggcccact tagccatttt tcaatcaata
    46621 cttcttcatc gtgctgaaat gccaatctta atgcatcttg tagagcattt tctgctacta
    46681 cttttgacat tcccacactg gaaccttcgc ggctcggctt aacgataacc ggcaaaccca
    46741 gagcagaaat ttctgctaac tgcttatcgc tcaggccttt ttcaaactct gcgcgggtta
    46801 acgctaccca cggcgcgacc ggtaaaccgg caccttgcca cagaagtttg ctgcgtagtt
    46861 tatctattga aagcgcagat gccatcactc cgctgccggt ataaggcaag cccatcagct
    46921 cgagcatgcc ctgcagcgta ccatcttcac cgccgcgacc gtgtagcgcg ataaacactt
    46981 tctgaaaacc catcgacttc agttgcgtca cgtcgacttc tttcgggtcg acaggatacg
    47041 catcaatacc gccttcacgc agcccggcta acaccgctgc gccagaattc agagaaactt
    47101 cccgctcagc ggaagtcccg cccaacagga ccgcgatttt atcagtcatg ttgttcttcc
    47161 tccggagttt gcggcttcag tttgatttca gctaaagaac gggcaatttt tccgatatta
    47221 ccagccccct gaacgagaat caggtcgtta ccggttaata ccggtgccag catctcggct
    47281 acctgcgcag ggtccggcac cagaatggga tcaattttcc cacgtccacg aattgtgcga
    47341 cacagcgaac ggctgtccgc tcccggaatt ggcgcttcgc cagccggata cacttccagc
    47401 atcaacaggg tatcaacctg cgtcagcaca ttggcgaaat catcatacag gtcgcgcgta
    47461 cgggtaaaac ggtgcggctg aaacagcatt accaggtttt tatccggcca gcctgcgcgc
    47521 gccgctttaa tggtggcgtc aacttccgtc gggtggtggc cgtagtcatc gaccagcatt
    47581 gccgtaccgc ttttaccatt cactggctcc agcgggaatt caccgaggaa atcaaaacgg
    47641 cgaccagtcc cctggaagct ctccagcgca cgcaaaatag cctcgtcgtc aattccctct
    47701 tccgtagcaa ccgcaaccgc agctgcggcg ttcagcgcgt tatgacggcc tggcgcattc
    47761 agggtgacgc acatcggctc tttgtcctga cgcagcagcg taaagtgccc ctgcgggcca
    47821 atctgctgat aatcttctac acgcacgtcg gcatcttcgc tgaagccgta agttgtggtc
    47881 tgacgtccca cacgcggtaa caattcgcgg atcaccggat catcaacaca catcaccgca
    47941 cgaccgtaaa aaggcaggtt gtgcagaaaa ttaataaagg tctgttttaa attctcaaag
    48001 tcgccctggt aggtatccat gtggtcggct tcgatattgg tgacaatcgc caccatcggt
    48061 tgcagatgca ggaacgatgc atcactctca tctgcttcgg caatcaggta ccgaccatgc
    48121 cccaaacgcg catgaacccc cgccgctttt accagcccgc cgttaacgaa ggttgggtcg
    48181 agccccgctt ctgcgtagat gctggaaacc atcgcggtag ttgtcgtttt gccgtgcgtt
    48241 cctgcaatgg cgatgccatg acgaaaacgc attaactcag ccagcatttc ggcacgacgg
    48301 atcaccggaa tacgcgcttc atgagctgcg acaatttccg ggttgtcggc agaaatggcg
    48361 ctggaaacaa cgaccacgct ggcatcacgt acgttttccg ggcgatggtt gaaataaatc
    48421 gtcgcaccca gattcagtaa ctgctgcgtg accggatttg gcgctaaatc ggaaccactg
    48481 atctgataac cttcattggc cagaacttcg gcaataccgc ccataccggc accaccaatg
    48541 ccgacaaaat gtatgtgccg aacgcgacgc atttcgggca cgatggaacg cagttttgcc
    48601 aattgttgtg tattcattct ttacgccatt aacttcttaa attcatacga tgcaaaaggc
    48661 atcgctacaa ttacgcccgg gcagcccggc tcacttcatt tgccactcgc tcggtggcat
    48721 ccggaatgga tgcagcgcgg gcgcgttctg ccatggttaa taaggtttct cgcgaccacc
    48781 cggccagggt gttggcgaca gcatccacgc taagctgtgg ctgctcgata attttggctg
    48841 cgcccgcttt ttccagcggt agcgcattcc agtattgctg gcggtcttta tgttgaaacg
    48901 gcacaaacaa cgccggtagt cctgccgcgg cgatttcact cacagttaaa gcaccggagc
    48961 ggcaaacgac gacatccgcc cacgcatacg ccgccgccat atcatcaata aattcagtca
    49021 ctttatgctg cggttgcccc gcttcggcat acgcctgttc aacggattgt tgcgaacctt
    49081 tgccgctctg atgccagata gtgactgaat cacccagttt cgcagcaacc tgcggcattg
    49141 tctggttaag aatgcgtgcg ccctgggaac cacccactac caacacacga accggacctt
    49201 cacgtccagc caaacgttgc tgcggcaacg gcagcgccag cacatcggta cgcaccgggt
    49261 tacccaccac ttccgcatta gggaaagcgc ctggaaacgc ctgcatcact ttggtagcaa
    49321 tcttcgccag ccatttattg gttaagcccg caataccgtt ttgttcatga agtacaaccg
    49381 gaatgcctaa cgaccacgcg gccagaccac ctggacctga tacatagccg cccataccga
    49441 gtaccacgtc aggtttgtac gctttcataa tcgcccgcgc ctgacgccag gcgttgaaga
    49501 tacgcagcgg cgcagctatc agtgctttta taccttttcc acgcaaacca gagatacgaa
    49561 tgaaatcaat ttcgatgcca tgttttggca ctaagtccgc ttccatacgg tcggcagtcc
    49621 ccagccagcg aacttgccaa ccctgagcca ttagatagtg cgcaaccgcc agtcccggga
    49681 atacatgtcc accggttccg cctgccatca ccattaatcg ctttccttga ccactcatcg
    49741 tgaacctcgt acaaacgcct gcgctttctc cagacgcgtt tcataatcaa tacgcaacag
    49801 catcatgatg gctgtcgaca taatcagtaa gctcgaacca ccgtaactga ttagcggcaa
    49861 tgtcagacct ttggtcggta acatccccgc cgccgcgcct acgttaacca gcgcctggaa
    49921 gctaaaccag atgccaatag aacaggcgag aaaaccggaa aaacggtggt caatttctaa
    49981 tgctttgcgg ccaatcgaca tcgcacgaaa agcgacgaag aataccatta aaagtgctag
    50041 taccacaccg acataaccta attcttcgcc gataatggcg aaaataaagt cagtgtgcgc
    50101 ttccggcaga tactccagtt tttgtaccga attacctaaa ccttgccccc aaagttcgcc
    50161 gcgaccaaac gccatcagcg attgcgttaa ctgatagccg ctgccaaagg gatcttccca
    50221 cgggttccag aatgcggtaa cacggcggat acggtacggt tcggcgagga tcagcaacac
    50281 aaccgctgaa atgcccatac cgataatggc aatgaactgc cacaatttcg ctcccgccag
    50341 gaacaacatc gccagcgtag tcacaaacaa caccaccacc gtaccaaggt ctggctgtgc
    50401 cagcagtaac actgccaaca ccagaatcac gcccatcggt ttcaggaagc cgcgcaggtt
    50461 attacgtact tcgtcgcctt tacgcaccag atagttggcg atatagcaaa acagcgacag
    50521 ttttgtcagc tccgcaggct ggatacgcag caaaccgaga tcgatccaac gcgatgcccc
    50581 tttaacagcg ctacccacta ccagcacgat catcagcagg atgatagatc cgagcagcat
    50641 cgtggcactg tagcgttgcc agaactccat cggcagacgc agcgtaatga tcgccagaat
    50701 aaacgccaaa atcagataga caccatcacg cttcgcgaag aagaacggat cgttggttaa
    50761 gcgttgccct atgggcattg acgccgaggt caccatgata aagccaatcg ccgcgaggcc
    50821 gaaggtcagc cacagtaagg tgcgatcgta catgatcagg ctgtcggtat ctttttcccg
    50881 cgagcccatc acccagccct ttagcgccgt ggagatccag accaggatac tgaatcctgg
    50941 caggcgcggc attttcaggc gagggagaga taaacgcatc aacctaactc cttcgccaga
    51001 cgggcaaact cattgcctcg ttgttcaaag ttcttgaact gatcaaggct ggcacaggcc
    51061 ggggagagca gaaccatatc gcccggctga acacgcggag ccagcaagcg catagcctgt
    51121 tccatagttt cggtttgttc tgccacttcc gggcgtagcg ccgccagctg cgcgccgtca
    51181 cgaccgaaac aatacagacg tacgttatcg ccattcaggt aacgcaccag tgggctaaag
    51241 tccgccgatt taccatcgcc gcccagcaac aaatgcagcg tgccgtctac gtgcaggcca
    51301 ttcagcgccg cttccgtact gccgacgttg gtcgcttttg aatcgttaat ccagcgcaca
    51361 ccgttatgct ccagcaccac ttcaaagcga tgcggcagac cggtgaaggt ggttaacgct
    51421 ttcaggctgc tggcgcgcgg taaccctgcg gcatctgcca gcgccagcgc caccagcgca
    51481 ttggtgtagt tatgctgccc ggaaagtttc atctctttca cattcagcac tttctcgcct
    51541 ttaacccgca gccaggtttc gccctgctga tgattcaggt gatagtcacc catgttgacg
    51601 ccaaagctga cgcagcgttc atccgcaccg cgaatcggca ttgttaaggc atcatcagca
    51661 ttaaccacgc aaactttcgc gttttcgtaa atgcgcagtt ttgctgcacg atactgttgt
    51721 aaaccaaacg ggtagcgatc catatgatct tcagtcacgt tcagaatggt cgctgctacc
    51781 gcctgtaagc tggaggtggt ttccagctgg aagctcgaca gttccagcac gtacagttca
    51841 cactcatcat ccagtagcat caacgcaggc aggccaatat tgccacccac accaacgtta
    51901 acccccgccg ctttcgccat ttcacccact agcgtggtga ccgtgctttt gccgttagaa
    51961 ccggtaatcg ccacaatcgg tgcttgtgct tcgcgacaaa acagctcgat atcgccaacg
    52021 atttcgattc cggcatcagc ggcagcgctt aaggatggat gcgccagtgc aataccggga
    52081 ctggcgacaa tcagatctgc cgccatcagc cattcatcat tcagactgcc cgtgtggcgt
    52141 tctacggctt cgggtaattt atccaggcca ggcggtgtca tacgcgtatc cataacgcgc
    52201 ggcgtcacac cgcgagcgag gaaaaagtcc acgcaggaaa gcccggtgag gcccaggccg
    52261 ataatgacaa catttttacc ctggtaatca gccatgatta acgtaccttc agcgttgcca
    52321 gaccaatcag gaccagcatc agcgaaataa tccagaaacg cacaatgacg cgcggttccg
    52381 gccagccttt cagttcatag tggtgatgaa tcggtgccat gcggaaaata cgttgtccgc
    52441 gcagtttaaa ggagccgacc tgcaggatga cagaaagcgt ttctaccacg aacacgcccc
    52501 ccataatcac cagcaggaat tcctgacgta gcagtacggc gataatgcct aacgcaccac
    52561 ctaacgccag cgaaccgaca tcgcccataa agacctgcgc cggataggtg ttaaaccaca
    52621 ggaagcccag tcctgccccg actatcgcgg tacagacaat aaccagttcc ccggcgtgtc
    52681 gcagatacgg tatgtgtaaa tagctggcaa agttcatgtt gccggtcgcc cacgccacca
    52741 gcgcaaaacc accggcgaca aatacggtcg gcataattgc cagaccgtca agaccatcgg
    52801 tcaggtttac cgcgttgcca gtacccacaa tgacgaagta agccagcaga atgtagaaca
    52861 gccccagttg cggcatcaca tctttaaaga atgggaccac cagctgcgtt gcgggcgtgt
    52921 ctttgccggc aaggtacaaa gcgaaagcaa cacccagcgc aatgaccgac atccagaaat
    52981 atttccaacg agcgatcaac cctttggtgt ctttacgcac cactttgcga taatcatcaa
    53041 caaagccaat aacaccgtaa cctaccagca ccaccaacac gcaccagacg tacggattgg
    53101 acgggtaagc ccacagcagc acggagatca caatcgctgt caggatcata atcccgccca
    53161 tggtcggcgt accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc actacctgac
    53221 caaaggaaag tttttgcaaa tgagcaatca tgcgcgggcc catccacaat gagatgaaca
    53281 gcgcggtcag caggctgacg atggcgcgaa acgtcagata ggaaaagacg ttaaagccgg
    53341 aataatattt gaccaaatgt tcggccagcc aaactaacat gtcccattct cctgtaaagc
    53401 gcgtactacc tcttccatgg cagcactacg tgaaccctta actaaaatcg taattacctg
    53461 ttgctcagca atcagtgatt taagacgcgt aattaacgca gttttatcag caaaatgttc
    53521 gccaacgccg ctggcggtgc tgatagcatg gctttgttta cccatgctta acacgcggtc
    53581 aataccagca gcttttgccg cctcacccac ctgtacatgg caggcttcgc tttcagcgcc
    53641 cagttccgcc atatcgccca ccaccagcac gcggtagccc ggcatttcag ccagtacctg
    53701 aacagctgcg gtcattgaac cgacattggc gttgtaggag tcgtcgagca gcaactggtt
    53761 ttctgccagt tggatgggga acagacggcc aggaacagct ttcagatttg ccagccccgc
    53821 tttgatagca tcaagcgttg cgcccacgga catggagagc gcagcggctg ccagcgcatt
    53881 cgcaatattg tgacgccccg gcaacggcag cagaacatcg acgctaccgg ttggggtttg
    53941 tagggtaaat tccataccgt gcgaggtcac atggatattg gtggcggtga aatcgctgtt
    54001 ggcggcattg ggtgagaaac gccacacttt gcgtgagcca attacgctct gccagttcag
    54061 ccagtcgttg ttgtcggcgt tcataatggc gataccgttt tccggcaggc cgctaaagat
    54121 ttcacctttc gctttcgcga cacccgcaag cgagccaaaa ccttccagat gcgccgctgc
    54181 caggttgttg accagcgccg cttccgggcg agtcagactc acagtccagg caatctcgcc
    54241 ctgatggttc gcgccaagtt caataactgc gtaatcgtat tccggcgtta agcgcaacag
    54301 cgtcatcggt acaccgatgt cgttgttgag attgcctgcc gtataaagcg tgttgccgca
    54361 ctggcttaaa atcgccgccg tcatctcttt aacggaggtt ttgccggagg accccgtcag
    54421 agcaaccacg agcgccggaa cttgctggcg aacccatgca gccagttcac caaacgccag
    54481 acgcgtatcc ttgacgatta actgcggcag gtcgatgtcc agcggacggc taaccagtag
    54541 tgcgcctgcg ccgccagctt tcgcctggtc ggcaaaatcg tgggcatcaa aacgttcacc
    54601 tttcagggca acaaacaggc agcccggcgt cagttttcgg gtatcagtgg ttacagcatc
    54661 aagggtgata tctgcacctt gcagttcacc gttgagaata tcggtaagtt ggctaagggt
    54721 tacgctaatc atgcaatccc ccccagcaga cgcgccaccg tgacgcgatc ggagtagtcc
    54781 agacgctgat tgccaacaat ctggtaatcc tcatggcctt tgcccgcgac cagtaccaca
    54841 tcattctctt tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc ttccatcact
    54901 ttggcatgtc cggcatctaa cattcccgcc agaatatcgt tgatgatggc acgcggttct
    54961 tcggtacgcg ggttatcgtc cgtcaccacc gccacgtcag caaactcttc ggcaattgcg
    55021 cccatcagtg gacgcttacc tttatcgcga tcgccaccac agccaaagac gcaccacagc
    55081 ttgcccgcac agtgcaggcg cgccgcctgt aaggcttttt ccagtgcatc cggcgtatgc
    55141 gcgtaatcca ccaccaccgt cggtttgcct ggcgcagtga acacttccat acgtccgcaa
    55201 accggttgca gacgcgcggc ggttttcagc agatcagcca gtggatagcc cagcgccagc
    55261 agtgttgcca gcgcgagcaa caggttgctg acgttaaaag cgcccatcag atggctttca
    55321 atttcgccat cgccccagct tgaactaaag cgaatggtcg cgccgctgtc gtgatagttc
    55381 acttcggtcg ctttcaacca gcgtccgtga cagttcggat tgatatgatc ttccattgat
    55441 accgcaaccg cgtccggcag ttttgccagc cagcggcggc ccacttcatc gtcggcgtta
    55501 ataatcgcct gaccgcaatg atgttcagaa taaagcagcc atttcgcagc ttcgtagtgt
    55561 tccatatcac catgataatc aaggtgatcg cggcttaagt tggtaaagac cgacgccgga
    55621 aatttcaatg ccgccacacg gtgctgtacc agcccgtggg aggaaacttc cattgcgcaa
    55681 aaagtcgcgc cctgatccac cagccccgcc agctcatgct gaacatcaac tgccgaaccg
    55741 gtagtatttt ctgtcgggat cactttcccc agcagaccgt taccaacggt gcccattacc
    55801 gcgctggttt cgccaagcag ttggctccac tgcgccagaa gctgggtcgt cgtggttttg
    55861 ccgttggtgc ccgttacgcc tacgagacgt aaattgtcag agggttcatg gtaaaagcgg
    55921 cccgccagtg cagataaacg ctcgttgagc tggctgagat agatgaccgg tacgccgtgc
    55981 atttcacgga tttcaccgtc ggtcgcctca tctttcgcct ctgcaataat ggcagccaca
    56041 ccttgcgcta tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct
    56101 acaaagagat cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc
    56161 gaaggtgcgt ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt
    56221 cccctcgcct tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc
    56281 gatgttcatg gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc
    56341 gccgccgtag tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg
    56401 ctgactcgca ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc
    56461 cggcccgacc tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc
    56521 cttcacgccg ccgccgcctg gtagcgccac gctttccatc atatgcacca cggtgcgaac
    56581 aatggattcc gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag
    56641 tgggcgataa atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt
    56701 taccattagc ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt
    56761 ttgaggatat aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc
    56821 aaaacgtgag taagtatcta ctaacgctga ggacggcatc gttaacgcca gcttggaaac
    56881 accgacgtta ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac
    56941 gtctttgatt tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg
    57001 caccacgcca cgttgcaacg cggtcatcac caccatcggt ttaaccgttg agcccggttc
    57061 aaacacgtcg gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt
    57121 agggttgtat gacgggctgt tagccatcgc cagcacttca cctgtgttga catccaccag
    57181 cacggcgcta ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac
    57241 cagcgcctgc aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt
    57301 agaagaaata tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg
    57361 ctgcccggta agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac
    57421 gttagtaaag ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc
    57481 ttcacgcaga tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagagtt
    57541 tacctgacgc gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggcagaaag
    57601 ctgatccagc ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat
    57661 accgccagcg tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac
    57721 cgctaacggg cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac
    57781 gcgaagagaa cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca
    57841 cgctacgcgt ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa
    57901 acgccaactg ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc
    57961 tgctttcatg cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc
    58021 aacatgctgc atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag
    58081 cgcattctct tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac
    58141 cagttgttcg cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacggtcac
    58201 cgccgtcaaa ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc
    58261 gtcaccgata acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt
    58321 gcttagagct tctgtcactc tgctgatcat gcgttcgtcc tctctgcaat acgcagaact
    58381 gaactacggg cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt
    58441 gctcgcagct gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc
    58501 ggaacttgcg gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc
    58561 gagtggaagc tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt
    58621 agcgcctgct ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta
    58681 cgggtcgcgg gatgtttaaa cttatctttc accggcgttg cagcagccac gacttccgcc
    58741 agttctttgg tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg
    58801 cgtttggcaa aatgctcttc accataggtt ttcaataccc aggcgatatc ggcttcttct
    58861 gcggtttgta gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc
    58921 ggaccatcgc gcataaagga aaagccgcgt tcagcatcat caagttgcgg tgaagagacg
    58981 ccaagatcga ggagaatgcc gtcgatcttg ccaataagat cgcgctcggc aacgtattcg
    59041 cccagcgcgg agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca
    59101 acggcgatag cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc
    59161 gagaggatca gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca
    59221 ggacggatat tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt
    59281 tccatcattt tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg
    59341 ctctgcgtcg atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa
    59401 cttgttgaac tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac
    59461 tggcgcgatt aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag
    59521 taggcgctgc acacggcgct caaccgggtt catgctcaac agacgcgata atttttgctc
    59581 gataatttcc cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt
    59641 gcaaaccatt tgaccggcag cgttctcaag cagctgttcc cgataacggg taggcactga
    59701 taagcgccct ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc
    59761 ccttattccc actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa
    59821 gcttgtcaag ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat
    59881 cagcgttgtt tattttacgt ccaacgtgcg aagcaaatta acgttatgaa tagctgtaag
    59941 aaaaaatccc tcatcgtcat cactgtttta aaatgactca ctattttcca cagcaaatat
    60001 aaagtgtcag tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc
    60061 ttgttacgtc tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgtt
    60121 tgttaaatcg gcaattcatc gccgcatgta acaaaataat gcaaaaacgc gctgtcgata
    60181 tcacttaatt attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaaccatt
    60241 tatcaataat agtcaggaat agtcttattt actttaagga tattgatg
//
LOCUS       DAJTYM010000011        59481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTYM010000011 DAJTYM010000000
VERSION     DAJTYM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            266..1555
                     /gene="sdaC"
                     /locus_tag="OO575_000701"
     CDS             266..1555
                     /gene="sdaC"
                     /locus_tag="OO575_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6663778.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1613..2980
                     /gene="sdaB"
                     /locus_tag="OO575_000702"
     CDS             1613..2980
                     /gene="sdaB"
                     /locus_tag="OO575_000702"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6663779.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3091..3846
                     /gene="xni"
                     /locus_tag="OO575_000703"
     CDS             3091..3846
                     /gene="xni"
                     /locus_tag="OO575_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6663780.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3899..5047)
                     /gene="fucO"
                     /locus_tag="OO575_000704"
     CDS             complement(3899..5047)
                     /gene="fucO"
                     /locus_tag="OO575_000704"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6663781.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5075..5722)
                     /gene="fucA"
                     /locus_tag="OO575_000705"
     CDS             complement(5075..5722)
                     /gene="fucA"
                     /locus_tag="OO575_000705"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6663782.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6269..7585
                     /gene="fucP"
                     /locus_tag="OO575_000706"
     CDS             6269..7585
                     /gene="fucP"
                     /locus_tag="OO575_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6663783.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7618..9393
                     /gene="fucI"
                     /locus_tag="OO575_000707"
     CDS             7618..9393
                     /gene="fucI"
                     /locus_tag="OO575_000707"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6663784.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9502..10921
                     /gene="fucK"
                     /locus_tag="OO575_000708"
                     /pseudo
     CDS             9502..10921
                     /gene="fucK"
                     /locus_tag="OO575_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            10923..11345
                     /gene="fucU"
                     /locus_tag="OO575_000709"
     CDS             10923..11345
                     /gene="fucU"
                     /locus_tag="OO575_000709"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6663785.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11403..12134
                     /gene="fucR"
                     /locus_tag="OO575_000710"
     CDS             11403..12134
                     /gene="fucR"
                     /locus_tag="OO575_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6663786.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12178..13278)
                     /gene="rlmM"
                     /locus_tag="OO575_000711"
     CDS             complement(12178..13278)
                     /gene="rlmM"
                     /locus_tag="OO575_000711"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6663787.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13271..13666)
                     /locus_tag="OO575_000712"
     CDS             complement(13271..13666)
                     /locus_tag="OO575_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6663788.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13685..14602)
                     /gene="gcvA"
                     /locus_tag="OO575_000713"
     CDS             complement(13685..14602)
                     /gene="gcvA"
                     /locus_tag="OO575_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6663789.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(14953..15180)
                     /locus_tag="OO575_000714"
     CDS             complement(14953..15180)
                     /locus_tag="OO575_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6663790.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15372..16577
                     /gene="csdA"
                     /locus_tag="OO575_000715"
     CDS             15372..16577
                     /gene="csdA"
                     /locus_tag="OO575_000715"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6663791.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16577..17020
                     /gene="csdE"
                     /locus_tag="OO575_000716"
     CDS             16577..17020
                     /gene="csdE"
                     /locus_tag="OO575_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6663792.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17071..17877)
                     /gene="tcdA"
                     /locus_tag="OO575_000717"
     CDS             complement(17071..17877)
                     /gene="tcdA"
                     /locus_tag="OO575_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6663793.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18115..19212)
                     /gene="mltA"
                     /locus_tag="OO575_000718"
     CDS             complement(18115..19212)
                     /gene="mltA"
                     /locus_tag="OO575_000718"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6663794.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19421..19497
                     /locus_tag="OO575_000719"
     tRNA            19421..19497
                     /locus_tag="OO575_000719"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19455..19457,aa:Met,seq:cat)
     gene            complement(19572..20825)
                     /gene="amiC"
                     /locus_tag="OO575_000720"
     CDS             complement(19572..20825)
                     /gene="amiC"
                     /locus_tag="OO575_000720"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6663795.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21057..22388
                     /gene="argA"
                     /locus_tag="OO575_000721"
     CDS             21057..22388
                     /gene="argA"
                     /locus_tag="OO575_000721"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6663796.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22450..24276)
                     /gene="recD"
                     /locus_tag="OO575_000722"
     CDS             complement(22450..24276)
                     /gene="recD"
                     /locus_tag="OO575_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6663797.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24276..27818)
                     /gene="recB"
                     /locus_tag="OO575_000723"
     CDS             complement(24276..27818)
                     /gene="recB"
                     /locus_tag="OO575_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6663798.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27811..30699)
                     /gene="ptrA"
                     /locus_tag="OO575_000724"
     CDS             complement(27811..30699)
                     /gene="ptrA"
                     /locus_tag="OO575_000724"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6663799.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(30875..34243)
                     /gene="recC"
                     /locus_tag="OO575_000725"
     CDS             complement(30875..34243)
                     /gene="recC"
                     /locus_tag="OO575_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6663800.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34256..34615)
                     /locus_tag="OO575_000726"
     CDS             complement(34256..34615)
                     /locus_tag="OO575_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6663801.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVLMVMIVTALSG
                     IQRTSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVS
                     PGGREGEMTRLHCPNCQ"
     gene            complement(34600..35007)
                     /locus_tag="OO575_000727"
     CDS             complement(34600..35007)
                     /locus_tag="OO575_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6663802.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35004..35567)
                     /locus_tag="OO575_000728"
     CDS             complement(35004..35567)
                     /locus_tag="OO575_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6663803.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35558..36028)
                     /locus_tag="OO575_000729"
                     /pseudo
     CDS             complement(35558..36028)
                     /locus_tag="OO575_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36212..37006)
                     /gene="thyA"
                     /locus_tag="OO575_000730"
     CDS             complement(36212..37006)
                     /gene="thyA"
                     /locus_tag="OO575_000730"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6663804.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37013..37888)
                     /gene="lgt"
                     /locus_tag="OO575_000731"
     CDS             complement(37013..37888)
                     /gene="lgt"
                     /locus_tag="OO575_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6663805.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38039..40285)
                     /gene="ptsP"
                     /locus_tag="OO575_000732"
     CDS             complement(38039..40285)
                     /gene="ptsP"
                     /locus_tag="OO575_000732"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6663806.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40298..40828)
                     /gene="rppH"
                     /locus_tag="OO575_000733"
     CDS             complement(40298..40828)
                     /gene="rppH"
                     /locus_tag="OO575_000733"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6663807.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41513..42202
                     /gene="mutH"
                     /locus_tag="OO575_000734"
     CDS             41513..42202
                     /gene="mutH"
                     /locus_tag="OO575_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6663808.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42271..42984
                     /locus_tag="OO575_000735"
     CDS             42271..42984
                     /locus_tag="OO575_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6663809.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43122..43340
                     /gene="ygdR"
                     /locus_tag="OO575_000736"
     CDS             43122..43340
                     /gene="ygdR"
                     /locus_tag="OO575_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6663810.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43448..44488
                     /locus_tag="OO575_000737"
     CDS             43448..44488
                     /locus_tag="OO575_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6663811.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44521..45711)
                     /gene="lplT"
                     /locus_tag="OO575_000738"
     CDS             complement(44521..45711)
                     /gene="lplT"
                     /locus_tag="OO575_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6663812.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45704..47863)
                     /gene="aas"
                     /locus_tag="OO575_000739"
     CDS             complement(45704..47863)
                     /gene="aas"
                     /locus_tag="OO575_000739"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6663813.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48449..49480
                     /gene="galR"
                     /locus_tag="OO575_000740"
     CDS             48449..49480
                     /gene="galR"
                     /locus_tag="OO575_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6663814.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49487..50749)
                     /gene="lysA"
                     /locus_tag="OO575_000741"
     CDS             complement(49487..50749)
                     /gene="lysA"
                     /locus_tag="OO575_000741"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6663815.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            50871..51806
                     /gene="lysR"
                     /locus_tag="OO575_000742"
     CDS             50871..51806
                     /gene="lysR"
                     /locus_tag="OO575_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6663816.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51793..52485)
                     /gene="ygeA"
                     /locus_tag="OO575_000743"
     CDS             complement(51793..52485)
                     /gene="ygeA"
                     /locus_tag="OO575_000743"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6663817.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52614..54032)
                     /gene="araE"
                     /locus_tag="OO575_000744"
     CDS             complement(52614..54032)
                     /gene="araE"
                     /locus_tag="OO575_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6663818.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54347..55108)
                     /gene="kduD"
                     /locus_tag="OO575_000745"
     CDS             complement(54347..55108)
                     /gene="kduD"
                     /locus_tag="OO575_000745"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6663819.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55138..55974)
                     /gene="kduI"
                     /locus_tag="OO575_000746"
     CDS             complement(55138..55974)
                     /gene="kduI"
                     /locus_tag="OO575_000746"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6663820.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56261..57442)
                     /locus_tag="OO575_000747"
     CDS             complement(56261..57442)
                     /locus_tag="OO575_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6663821.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57697..58926
                     /locus_tag="OO575_000748"
                     /pseudo
     CDS             57697..58926
                     /locus_tag="OO575_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14674 a        15977 c        14506 g        14324 t
ORIGIN      
        1 gggcaaatat tgcccttaaa ttctctttta cttttgattt acagagtaga gcgttgggat
       61 aatctatctt ccaagcagat tattgtattt gggatcaaga tcactgatag atacataact
      121 tgtgtgtatc tttccgccct caaattatta cggcggtaaa tgattaagcc atcgccgata
      181 gacagatttc atttttacgg tcaggcacct tcccgggctg aactggctaa aagctgaatt
      241 atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga ttgcgtcgaa
      301 agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc tttacggcac
      361 ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc
      421 gctgatcatc atggctatcc ttgcgttccc gatgacgttt tttgctcacc gcggcctgac
      481 tcgcttcgta ctgtctggta aaaacccggg cgaagacatc accgaggttg tagaagaaca
      541 ctttggtatt ggcgcaggta aactgattac cctgctctac ttcttcgcta tctacccgat
      601 cctgctggtt tatagcgtgg caatcaccaa taccgttgaa agcttcatgt ctcaccagct
      661 gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac
      721 catcgttcgc tttggtgagc agatgatcgt taaagcgatg agtattctgg tattcccgtt
      781 tgttggcgta ctgatgctgc tggctctgta cctgatcccg cagtggaacg gcgcagcact
      841 ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg
      901 gctggcaatt ccggtaatgg tgttctcgtt caaccactct ccgatcatct cttctttcgc
      961 cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga agatcctggc
     1021 attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct gcgtactgag
     1081 cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc tgtcttacct
     1141 ggctaaccac tttaacgcac cggttatcgc gtggatggct ccgattatcg cgattatcgc
     1201 tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca acggtatggt
     1261 gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga accgtatcac
     1321 tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga gcatcctggg
     1381 tatgattgaa accctgggcg gcccaatcat cgcgatgatc ctgttcctga tgccgatgta
     1441 cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt cacatcagca acgtattcgt
     1501 tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca gctaagtcct
     1561 ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc ctatgattag
     1621 cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg ttggaccaat
     1681 gaaagcgggt aaacaattta ccgacgatct gattgcccgt aacctgctta aagacgtgac
     1741 ccgcgtggtg gttgacgtgt acggctcgct ctctctgact ggtaaaggcc accacacaga
     1801 tatcgccatt attatgggcc tggcgggtaa cctgccggat accgtggata tcgattccat
     1861 ccccggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa acggtcagca
     1921 tgaagtggag ttcccggttg atcagtgcat gaacttccat gcagacaacc tttctctgca
     1981 tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca gccagactta
     2041 ctactctatt ggcggtggct ttatcgttga tgaagagcat tttggccagc aggatagcgc
     2101 accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac attgtcagga
     2161 aaccgggctg tcactctctg gcctgatgat gaaaaacgag ctggcgctgc acagtaaaga
     2221 agagctggaa cagcacctgg cgaacgtctg ggaagtcatg cgtggcggta ttgagcgcgg
     2281 tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact
     2341 acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg ttgttgactg
     2401 gatcaacatg tttgcactgg cagtgaacga agagaacgct gctggcggtc gcgtggtgac
     2461 tgcgccgact aacggtgcgt gcgggattat cccggctgtg ctggcgtact acgataagtt
     2521 tatccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca gcgccattgg
     2581 ttctctttat aagatgaacg cgtcgatttc tggtgccgaa gtgggttgcc agggtgaagt
     2641 tggcgtggcg tgctctatgg cggcagctgg tctggcagaa ctgttaggcg caagcccggc
     2701 gcaggtttgc atcgcagcgg aaatcgcgat ggagcacaac ctcggtctga cttgtgaccc
     2761 ggtcgcgggg caggttcagg taccgtgcat tgagcgtaac gccattgcag cagtgaaagc
     2821 ggtgaacgcc gcgcgtatgg cactgcgccg taccagcgaa ccgcgcgtct gcctcgataa
     2881 agttatcgaa accatgtacg aaacaggtaa agacatgaac gccaagtacc gcgaaacctc
     2941 tcgcggcggc ctggcgatga agatcgttgc ctgcgattaa tctctcccag aggcctcgtt
     3001 ttgcgaagcc tcttcctgtt ttctcaacca gccatagcct gttccggcat cgaatgttac
     3061 ccttatcgcc tgatctttaa gggggttatc gtggctgttc atttgcttat tgtcgatgca
     3121 ctgaatctta tccgtcgcat tcatgccgtt cagggttcgc cctgtgtcga aacctgccag
     3181 catgcgctcg atcagctcat tatgcacagc cagccaaccc acgcggtcgc cgtttttgat
     3241 gatgaaaacc gcagtagcgg ctggcgtcat cagcgtttac cagattacaa agcggatcga
     3301 ccgccaatgc cggaagagtt gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa
     3361 cgcggcgtcc cgtgctggtc agccagcggc aacgaagccg atgacttagc cgccacactg
     3421 gcggtcaaag tgacacaggc cgggcatcag gcaacgattg tttcgacaga taaaggctac
     3481 tgtcagttac tttcaccgac attacgtatt cgtgattact tccagaaacg ttggctggat
     3541 gcgccattta tcgataaaga atttggcgtt caaccgcagc agttgcccga ttactgggga
     3601 cttgcgggga tcagcagttc aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc
     3661 acgcagctgc tggtcgagtt tcagagtctg gaagggatat atgagaatct ggatgcggtt
     3721 gccgaaaagt ggcgcaaaaa attagaaacc cataaagaga tggcgtttct gtgccgcgat
     3781 attgcccgct tacaaaccga tttgcatatc gacggcaatt tacagcaatt gcggttggta
     3841 cggtaacggc gagccggata cgccgcaaat cgtatccggc attatcacat cagcgcattt
     3901 accaggcggt atggtaaagc tctacaatat cctcaagcgt tgcttcacgc gggttgccac
     3961 cggtacaaac atcatccagt gccgcctgag ccagtgccga aatgtcttcc ttgcgtacac
     4021 caacatcacg caaatgtggc ggaataccga catcacggtt gagggcaaac accgcttcaa
     4081 cagcggcatt acgcgcccct tccaggctca taccttccac tttcacgccc ataacgcgcg
     4141 cgatatcgcg gtacttctca ccggtaaagt cagcgttata gcgcatgaca tgcggtaaca
     4201 ggatggcgtt cgcaacaccg tgtggagtgt tataaaacgc gcccagtgga tgcgccatac
     4261 catgcaccaa ccctaaccca acattcgaga agcccatacc cgcaacatac tgcccgagcg
     4321 ccatttcttc tccggcatcc ttatcaccag caaccgatcc tcgcagcgcc ccagcaatga
     4381 tttcaatcgc tttaatgtgc agtgcatcgg ttagtgccca cgcgccacgg gtaatatacc
     4441 cctcaatagc atgagtgagc gcatcaacac ccgtcgcggc tttcagcgcc ggaggcatac
     4501 catccatcat gtcagcatca ataaacgcca cctgcgggat atcatgcgga tcaacgcaaa
     4561 caaacttgcg ccgtttttct tcgtcagtga tcacgtagtt aatggtcact tctgccgcag
     4621 tacctgctgt ggtggggatt gccagaatcg gtacactggg tttattggtc ggggaaagcc
     4681 cttccaggct acgcacatcg gcaaactccg ggttgttgct gataatgcca attgctttac
     4741 aagtatcctg tggagaacca ccaccaatag cgatcaggta atccgcgccg ctattctgga
     4801 atacaccgag cccttctttg acgacagtaa ttgttgggtt gggcactacg ccgtcgtaaa
     4861 tcgcccatgc cagccctgca gcatccatct tatcggtcac tttcgccacc acgccgcatt
     4921 gcaccagcgt tttatcggtg acgatcagcg ccttctgata accacggcgt ttcacctcat
     4981 cggttaaagc cccaacagca ccccgaccaa accatgccgt ttcgttcaga atcattctgt
     5041 tagccatcat ccttctcctt gttgctttac gaaattactc ttcaatccgt aacccatagg
     5101 ttttgaattt ctccagcact acggcaatct cttcatcgct cagcactggc accgggtccg
     5161 taatcgccag ggtcgtcagg taaagttgcg ccagcacttc aacttcatgc gccagccata
     5221 acgctttttc cagattcacc tcacaagcga taagcccatg atgttgtaac aaagttgcct
     5281 tacgattttt gagagccagc gcaacatgtt cagaaagttc gcgtgttcca aaggtcgcat
     5341 aaggcgcgca aggaatagaa ttaccgccag ccgccgcaat catgtagtga atagcgggga
     5401 tcgatcggtt aagaatggaa actgccgtgc aatgaacggc atgattgtga acaaccgcgt
     5461 tggcatccgg tctgctttga taggctgcca tatggaaacg ccattcgctt gaggggagct
     5521 ttccttcctc atgtttaccg ttgccatcaa taaagacaat atgcgactcc gtcagttttt
     5581 catatggaat gcctgtaggc gtaatcagca tcccatcctg ataacgtaca ctgacgttcc
     5641 ccgctgtccc ctggttcagt cccaggcggg tcatttccag gcaagtgtca ataatctgac
     5701 gagcaagttt atttcgttcc attagctacc tctctctgat tcaaaacagg gcaataatgt
     5761 tgttcctttc acactattga attagccgtt taattaccca ccatcttctt cctgattaac
     5821 aagaaagaaa ttcacaagct tatattttgt gacctagctc aactaatcac agtaaataac
     5881 tgcaagttct ctttttataa ccccgttaaa aatgaccgct cttaaaaata tttatcaaaa
     5941 cggtcatttt tccatttccc ctcgccagaa atgcccgttt tcggcacaaa caattaatac
     6001 ggtcatctgg tttgtgtttt ttatgattta ttttctgaaa cgggcatgaa atttcgatta
     6061 ttaaagtgat ggtagtcaca taaagtcacc ttccagctaa taagtgtgac cgccgtcata
     6121 ttacagagcg ttttttattt gaaaatgaat ccatgagttc atttcagaca ggcaaatatt
     6181 caccgatatg aagcccgaac tcgctggttt tgcacttttg aaaacataac cgattacgtg
     6241 cttaagcttc tgaacctaag aggatgctat gggaaacaca tcaatacaaa cgcagagtta
     6301 ccgtgcggta gataaagatg cagggcaaag cagaagttac attattccat tcgcgctgct
     6361 gtgctcactg ttttttcttt gggcggtagc caataacctt aacgacattt tattacctca
     6421 attccagcag gcttttacgc tgacaaattt ccaggctggc ctgatccaat cggcctttta
     6481 ctttggttat ttcattatcc caatccctgc tgggatattg atgaaaaaac tcagttataa
     6541 agcagggatt attaccggat tatttttata tgccttgggc gctgcattat tctggcccgc
     6601 tgcagaaata atgaactaca ccttattttt agttggccta tttattattg cagccggatt
     6661 aggttgtctg gaaactgccg caaacccttt tgttacggta ttagggccgg aaagcagtag
     6721 tcacttccgc ttaaatcttg cgcaaacatt taactcgttt ggcgcaatta tcgcggttgt
     6781 ctttgggcaa agtcttattt tgtctaacgt gccacatcaa tcgcaagacg ttctcgataa
     6841 aatgtctcca gagcaattga gtgcgtataa acacagcctg gtattatcgg tacagacacc
     6901 ttatatgatc atcgtggcta tcgtgttact ggtcgccctg ctgatcatgc tgacgaaatt
     6961 cccggcattg cagagtgata atcacagtga cgccaaacaa ggatcgttct ccgcatcgct
     7021 ttctcgtctg gcgcgtattc gtcactggcg ctgggcggta ttagcgcaat tctgctatgt
     7081 cggcgcacaa acggcctgct ggagctattt gattcgctac gctgtagaag aaattctagg
     7141 tatgactgca ggctttgccg ctaactattt aaccggaacc atggtgtgct tctttattgg
     7201 tcgtttcacc ggtacctggc tcatcagtcg cttcgcacca cacaaagtcc tggccgccta
     7261 cgcattaatc gctatggcac tgtgcctgat ctcagccttc gctggcggtc atgtgggctt
     7321 aatagccctg actttatgca gcgcctttat gtcgattcag tacccaacaa tcttctcgct
     7381 gggcattaag aatctcggcc aggacaccaa atacggttcg tccttcatcg ttatgaccat
     7441 tattggcggc ggtattgtca ctccggtcat gggttttgtc agtgacgcgg cgggcaacat
     7501 ccccactgct gaactgatcc ccgcactctg cttcgcggtc atctttatct ttgcccgttt
     7561 ccgttctcaa acggcaacta actgaacata ttttccgaat aaagtgagga atctgtaatg
     7621 aaaaaaatca gcttaccgaa aattggtatc cgcccggtta ttgacggtcg tcgcatgggt
     7681 gttcgtgagt cgcttgaaga acaaacaatg aatatggcga aagctacggc cgcactgctg
     7741 accgagaaac tgcgccatgc ctgcggagct gccgtcgagt gtgtcatttc cgatacctgt
     7801 atcgcgggta tggctgaagc cgctgcttgc gaagaaaaat tcagcagcca gaatgtaggc
     7861 ctcaccatta cggtaacgcc ttgctggtgc tatggcagtg aaaccatcga catggatcca
     7921 acccgcccga aagccatttg gggctttaac ggcactgaac gccccggcgc tgtttacctg
     7981 gctgcggctc tggcagctca cagccagaaa ggcatcccgg cattctccat ttacggtcat
     8041 gacgttcagg atgccgatga cacatcgatt cctgccgatg ttgaagaaaa actgctgcgc
     8101 tttgcccgcg ccggtttggc cgtcgccagc atgaaaggta aaagctatct gtcgctgggc
     8161 ggcgtttcga tgggtatcgc cggttctatt gttgatcaca acttctttga atcctggctg
     8221 ggaatgaaag tccaggcggt ggatatgacc gaactgcgtc gccgtatcga tcagaagatt
     8281 tacgacgaag ccgaactgga aatggcactg gcctgggctg ataaaaactt ccgctatggc
     8341 gaagatgaaa ataacaaaca gtatcaacgc aatgccgagc aaagccgcgc agttctgcgc
     8401 gaaagtttac tgatggcgat gtgtatccgc gacatgatgc aaggcaacag caaactggcc
     8461 gatattggtc gcgtggaaga atcacttggc tacaacgcca tcgctgcggg cttccagggg
     8521 caacgtcact ggaccgatca atatcccaat ggtgacaccg ccgaagcgat cctcaacagt
     8581 tcatttgact ggaatggcgt gcgcgaaccc tttgtcgtgg cgaccgaaaa cgacagtctt
     8641 aacggcgtgg caatgctaat gggtcaccag ctcaccggca ccgctcaggt atttgccgat
     8701 gtgcgtacct actggtcacc agaagcaatt gagcgtgtaa cggggcataa actggacgga
     8761 ctggcagaac acggcatcat ccatttgatc aactcaggtt ctgctgcgct ggacggttcc
     8821 tgtaaacaac gcgacagcga aggtaacccg acgatgaagc cgcactggga aatctctcag
     8881 caagaggctg acgcttgcct cgccgctacc gaatggtgcc cggcgatcca cgaatacttc
     8941 cgtggcggcg gttactcttc ccgcttcctt accgaaggcg gcgtcccgtt caccatgact
     9001 cgtgtcaaca tcatcaaagg cctgggaccg gtactgcaaa tcgcggaagg ctggagcgtg
     9061 gaattgccga aggatgtgca tgacatcctc aacaaacgca ccaactcaac ctggccaacc
     9121 acctggtttg caccgcgcct cactggtaaa gggccgttta cggatgtgta ttcggtaatg
     9181 gcgaactggg gtgccaacca cggagttctg accatcggcc acgttggcgc agactttatc
     9241 acccttgcct ccatgctgcg tatcccggta tgtatgcaca acgttgaaga gaccaaagtg
     9301 tatcgtcctt ctgcctgggc tgcgcacggc atggatattg aaggccagga ttaccgcgct
     9361 tgccagaact acggtccgtt gtacaagcgt taatacatta caccctgatg tgatgcccgg
     9421 tcgctccggc taccgggcct gaacaagcaa gagtggttag ccggataaac aatgttatcc
     9481 ggctatattg caggagcgat tatgaaacaa gaagttatcc tggtactcga ctgtggcgcg
     9541 accaatgtca gggccatcgc ggttaatcgg cagggaaaaa ttgttgcccg cgcctcaacg
     9601 cctaatgcca gcgatatcgc gatggaaaac aacacctggc accagtggtc tttagactgc
     9661 cattttgcaa cgctttgctg attgctgtcg gcaaatcaat agtgaactga ctgaatgcca
     9721 catccgcggt atcgccgtca ccacctttag tgtggatggc gctctggtag ataagcaagg
     9781 caatctgctc tatccgatca ttagctggaa atgtccgcga acagcagcgg ttatggacaa
     9841 tattgaacgg ttaatctccg cacagcggtt gcaggctatt tctggcgtcg gagcctttag
     9901 tttcaatacg ttatataagt tggtgtggtt gaaagaaaat catccacaac tgctggaacg
     9961 cgcgcacgcc tggctcttca tttcgtcgct gattaaccac cgtttaacca gcgaattcac
    10021 tactgatatc acgatggccg gaaccagcca gatgctggat atccagcaac gcgatttcag
    10081 tccgcaaatt ttacaagcca ccggtattcc acgccgactc ttccctcgtc tggtggaagc
    10141 gggtgaacag attggtacgc tacagaacaa cgccgcagca atgctcggct tacccgttgg
    10201 cataccggtg atttccgcag gtcacgatac ccagttcgcc ctttttggcg ctggtgccga
    10261 acaaaatgaa cccgtgctct cctccggtac atgggaaatt ttaatggttc gcagcgccta
    10321 ggttgatact tcgctgttaa gtcagtacgc cggttccacc tgcgaactag atagccaggc
    10381 atggttgtat aacccaggta tgcaatggct ggcatccggc gtgctggaat gggtgagaaa
    10441 actgttctgg acggctgaaa caccctggca aatgttgatt gaagaagctc gtctgatcgc
    10501 acctggcgcg gatggtgtga aaatgcagtg tgatttattg tcgtgtcaga acgctggctg
    10561 gcaaggagtg acgcttaata ccacgcgggg gcatttctat cgcgcggcgc tggaagggtt
    10621 aactgcgcaa ttacagcgca atctacagat gctggaaaaa atcgggcact ttaaagccta
    10681 tgaattattg ttagtcggtg gaggaagtcg caacacattg tggaatcaga ttaaagccaa
    10741 tatgcttgat attccggtaa aagttctcga cgatgccgaa acgaccgtcg caggagctgc
    10801 gctgttcggt tggtatggcg taggggaatt taacagcccg gaagaagccc gtgcacagat
    10861 tcattatcag taccgttatt tctacccgca aactgaacct gaatttatag aggaagtgtg
    10921 aaatgctgaa aacaatttcg ccgttaattt ctcccgaact attgaaagtg ctggcagaga
    10981 tgggacatgg agatgaaatt attttttccg atgctcactt tcccgcccat tcgatggaac
    11041 cgcaggtgat ccgcgctgat ggcctgttgg tgagcgactt gctccaggcg attatcccgt
    11101 tatttgagct ggacagttat gccccgccgc tggtgatgat ggcggcggta gagggtgaca
    11161 ctctcgatcc tgaagtagaa cgacgttacc gtaatgcgct ttcactacaa gccccgtgtc
    11221 ctgacatcat ccgcatcaat cgttttgcgt tttatgaacg ggcgcaaaaa gcctttgcga
    11281 tcgttatcac aggcgaacga gcgaagtacg ggaatattct tttaaaaaaa ggggtaacac
    11341 cgtaatctca taccggtacg cccgcatgac gcgggcggtt atcgaatgat ggggtgaaaa
    11401 atatgaaagc ggcacgccag caagcgatag tcgacctgct gctgaaccat accagcctga
    11461 ccacggaagc tctctctgaa cagctaaagg tcagtaaaga aaccattcgt cgcgatctca
    11521 atgaattaca gacgcagggt aaaattctgc gcaatcatgg acgcgctaaa tatatccacc
    11581 gtcaaaatca agacagtggc gatccctttc acatcaggct gaaaagccat tatgcgcata
    11641 aagcagatat cgcgcgcgag gcgctcgcgt ggattgaaga agggatggtg atagccttag
    11701 acgccagttc aacttgctgg tatctggcac gccagttgcc tgacatcaac attcaggtct
    11761 tcaccaatag ccatccgatt tgccatgaac tcggtaaacg cgaacgcatt caactgatca
    11821 gttccggcgg cacacttgag cgcaaatatg gctgttacgt caatccctcg ctgatttccc
    11881 aacttaaatc gctggaaatc gatctgttta ttttttcttg tgaagggatc gatagcagcg
    11941 gcgcactgtg ggactccaat gcgatcaacg ctgattacaa atcgatgcta ttaaaacgtg
    12001 ccgcgcaatc gttgttattg attgataaaa gtaaatttaa tcgttcaggg gaagcccgca
    12061 tcgggcatct ggatgaggta acgcacatta tttctgatga gcgccaggtt gcaacttctt
    12121 tggtaacagc ctgacggaaa gggtagcagg ccggagacga cgccccggcc ttgcctgtta
    12181 tcgctcgtcg cgacgaccac ccaccgcagc ccagatgcgg cggacgtgca ccgtcacttc
    12241 ttcgcgatcg tgatacaact gccgcgcctg aatctgagca tttatgccat gttcatcaag
    12301 ctgtgcctga atatacgcca gattgtgtga tacttcttcg tagcgttttt tcatcggcag
    12361 tttgagattg aaaatggttt cacggcacca gccattaacc agccactgcg ccatcaatgc
    12421 cgcaacttta gccggttttt caaccatatc gcataccatc caggagatat tgctgcgtgt
    12481 cggacggaat ttgaaaccgt cttcccgcag ccacgtcacc tgcccggtat ccatcagact
    12541 ttgcgccatc gggccgttgt cgacggaata aacccacatg ttgcgcttca ccagttggta
    12601 ggtccagccg ccagggcaag cgcctaaatc caccgcccac atcccgttcg ccaggcgttc
    12661 atcccactca tccgcaggaa taaacacatg aaatgcctct tccagtttga gcgtggaacg
    12721 actcggcgcc tctgccggaa atttcaggcg cggaatgccc atatagaacg gcgaattatt
    12781 gttgctgtat gagtaaccgg tatagcagca tcctggtgca ataaagaata catgcacaac
    12841 cggacgcttc ggcgtttcat agttcgccag caccccagca tcgcgcaagg cagcgcgtag
    12901 cggaacagtg aacttacggc agaatttcag caactctttg ctttcgttgg tatcggcaac
    12961 ttcaacacgc agttcaccgc ccttctctac tacaccctgt aacatgccga caatgggggt
    13021 aatacgatct tctggcggca aatgctgcag gagttccccc accacaaacc actggcgggc
    13081 aaaaattaat gaactgaacg gcagctcacg gattaactta tcgccatcat caggttgata
    13141 acattcataa atgacataac ccgcattctc tttcacgcgg gcaaaaccga aaatttcccg
    13201 ctggccggct ttatcggtaa tttctgcggc gcactctttt tcaaagcccg gacggcacag
    13261 caatacaacc ttattcatga cttacgccct tgcgctttaa acggatagca ccaactaaca
    13321 ttaacgccca gcccgcaagg aagctcacgc cgccaaccgg tgtgacaaac gcccacaaac
    13381 gcagatggga cagcgccagg caataaaggc tgccgctgaa cagcaccgtg cctaacgcga
    13441 ggaaaacgct actccagtaa aaccagatac tgatgcgacg ctgcattgcc accgccagac
    13501 ctaagatcgc cagcgtatga aacgcctggt attcgagacc ggtttggatc cagcccatct
    13561 caacggcccc catggtttta cttaacacat gcgcaccaaa agcgcccagt gccacaaaaa
    13621 tgaagccgct aatggcggcg aaaatcagca taaaacggct ggtcatggtc gtaccctacg
    13681 taaattattg ttcataacga aagcggaatt tttcttgttc agcagcggct ttcgccagga
    13741 tccattggcg aaaggcggct attttaccca gttctgcctg gctgtcatga caaaccagat
    13801 aaaaagcatt tttactgacc agaacatcat taaacgggca aacaagacgt ccggcctcga
    13861 tttcagattg cgccatcacg ttatttgcca gcgccactcc ctgcccgtga atagccgctt
    13921 gcagcaccat ggcgctatga ctaaaaattg gcccttgctg aacgttgata tgattaaacc
    13981 ccaactgtcg ggtatatgtc tgccagtcac ggcgtgacgc atcatgtaat aacgtatgtt
    14041 tagccagatc ttccggggtc ttcaagggtt tttcgcctgt cagcagtagc ggcgaacaca
    14101 ccggcaataa atattcggcg tacagttttt ccacccgtag ccccggccag ttgccccgac
    14161 cataaaatat cgccacatca acatcatccg ccagcttatc ttcctgacga tcaaccgcct
    14221 ggattcgaac gtcaattccc ggataagctg aattaaagct ggaaagtcgc ggaaccaacc
    14281 aatgaatggc gaaactgggg agtaaactga ccgtcaacgc ccccttggcg ctacgggcct
    14341 ggagtttacg cgtcgcttcg gttaattgcg aaaatatctc tttgatatcg aggaaatagc
    14401 tttgcccttc ctcggtcagc aggagtgaac gattacggcg gcggaacagt tttagcccca
    14461 aaaaatcctc aagagacttg atttgatgac ttactgcggc ctgggtcaca aaaagctctt
    14521 ctgctgcgcg agtaaaactt aaatggcgtg ctgcggcatc aaaaactcgt aaggcattta
    14581 gcggtggtaa tcgtttagac atggctttta aactttgatg ttaaatgaat ttaacaatta
    14641 gatcacactg tgtaacctat tagttttttt aatctgagcc attataaatt gtccgttgag
    14701 cttctaccag caaataccta tagtggcggc acttcctgag ccggaacgaa aagttttatc
    14761 ggaatgcgta ttctggtgaa cttttggctt acggttgtga tgttgtgttg ttgtgtttgc
    14821 aattggtctg cgattcagac catggtagca aagctacctt ttttcacttc ctgtacattt
    14881 accctgtctg tccatagtga ttaatgtagc accgcctaat tgcggtgctt ttttttacct
    14941 tgcgatcgcg aattactgtt ccagttcgac catctctttc acgtcagtac ggttgatctg
    15001 ctgtttgttg ccattagcgt ctttatacga aatcataccg atatcgttat cagtctgtgg
    15061 tttgccgtca gagacgatgg tacgtccgtc attggtgtgc atcacatagt tcgaaccgga
    15121 acaggcgctc agggcaaaag tcagcataca ggcagaaata attgcggcag tctttttcat
    15181 catcttctcc ttaaaacgat taataaatct taatagtatt gattaacagg ctaaaattaa
    15241 cgcctaacac tattcagcat atgttactta cgcggcttcg ccaggatatc cagataattc
    15301 tgatggttag cactctcctt gtatcaaagt gaattttgcg tcacgatcgg tgcatcaagc
    15361 cgaggagtac catgaacgtt tttaatcccg cgcagtttcg cgcccagttt cccgcactac
    15421 aggatgcggg cgtctatctc gacagcgccg cgaccgcgct taaacctgaa gccgtggttg
    15481 aagccactcg acagttttac agcctgagcg ccggaaacgt ccatcgcagc cagtttgccg
    15541 aagcccaacg cctgaccgcg cgttatgaag ctgcacgaga gaaagtggcg caattactga
    15601 atgcaccgga tgataaaact atcgtctgga cgcgcggcac cactgaatcc atcaacatgg
    15661 tggcacaatg ctatgcgcgt ccgcgtctgc aaccgggcga tgagattatt gtcagcgtgg
    15721 cagaacacca cgctaacctc gtcccctggc tgatggtcgc ccaacaaact agagccaaag
    15781 tggtgaaatt gccgcttaat gcgcagcgac tgccggatgt cgatttgttg ccagagctga
    15841 ttactccccg tagtcggatt ctggcgttgg gtcagatgtc gaacgtgact ggcggttgcc
    15901 cggatctggc gcgagcgatt acctttgctc attcagccgg gatggtggtg atggttgatg
    15961 gtgctcaggg gacggtgcat ttccccgcgg atgttcagca actggatatc gatttctatg
    16021 ctttctctgg tcacaaactg tatggcccga cgggtatcgg cgtgctgtat ggcaaatcag
    16081 aactgctgga agcgatgtcg ccctggctgg gtggcggcaa aatgattcac gaagtgagtt
    16141 ttgacggttt caccactcaa tctgcgccgt ggaaactgga agcaggaacg ccaaatgtcg
    16201 ctggcgtcat aggattaagc gcggcgctgg aatggctggc agattacgat atcaaccagg
    16261 ccgaaagctg gagccgtagc ttagcaacgc ttgcagaaga agcgctggcg aaacgtccag
    16321 gctttcgttc attccgctgc caggattcca gcctgctggc ctttgatttt gctggcgttc
    16381 atcacagcga tatggtgacg ctactggcgg agtacggtat tgccttgcgg gccgggcaac
    16441 attgcgctca gccgctactg gcagaattag gcgtaaccgg cacactgcgc gcctcatttg
    16501 cgccatataa tacaaagagt gatgtggatg cgctggtgaa tgccgttgac cgcgcgctgg
    16561 aattattggt ggattaatga caaacccgca attcgccgga catccgttcg gcacaaccgt
    16621 aaccgcagaa acgttacgca ataccttcgc accgttgtcg caatgggaag ataaatatcg
    16681 ccagttgatc atgctgggga aacagcttcc ggctttgcca gacgagttaa aagcgcaggc
    16741 taaagagatt gccggatgcg aaaaccgcgt ctggctggga tataccgtgg ctgaaaacgg
    16801 caaaatgcac ttctttggca acagcgaagg gcgcattgtg cgcggcctgt tggcggtgtt
    16861 gttgactgcc gttgagggga aaaccgccgc cgaattacag gcacagtcgc cgctggcatt
    16921 gtttgatgag ctgggattac gtgcgcagct tagcgcctca cgcagccagg ggttaaatgc
    16981 gttaagcgag gcgattatcg ctgcgacgaa gcaggtttaa gcacagaacc gggtcggata
    17041 agacgttcgc gtctcatccg acctgattgt ttaaccctga cgcgccgctt tcgccatcat
    17101 cttcttcagc gcatgagaaa ccgcaacaaa cccaaaggtg gcggtcacca tcgttgccgc
    17161 gccaaatcca gatgcacaat ccatccgctt cggcccttct gccgtggctt tcatcgcaca
    17221 caccgtaccg tctgactgcg ggtacaccag cgcttcagta gaaaacacgc aatccacgcc
    17281 gagcttacct ttactgtttt tcactacgcc aaaatcgctt ttcaggcgct cgcgcaactt
    17341 cgccgccagc ggatcctgaa tcgttttcgc cagatcggta acctgaatct gcgtcgggtc
    17401 aatctgccca cccgcgccac cggtcgtgac cagcgggatt ttattgcgcc gacaataagc
    17461 aatcagcgcc gctttgggcc gtacactatc aatggcatca atcacgtacg aataaccggc
    17521 actcatatat tgcgctacgt tatccggcgt cacgaaatca tccaccaccg ttacacggca
    17581 ctccgggtta atctggcgaa tgcgctccgc catgacttcc gcttttgcca gcccgacgtt
    17641 atcgcgcaga gcgtgaattt gccgattggt attggtgacg cacacatcat ccatatcgat
    17701 aagcgtgatt gcgccaatcc ccgtgcgcgc cagcgcttcc gccgcccagg aaccgacacc
    17761 accgatacca accacacaaa tatgcgcgtc agcaaacagt tgcaacgctt tttcaccata
    17821 cagacgcgct gtgccaccaa aacgctgacg ccatgcatca ctaattacca cagacatagc
    17881 acctcagaat taaaaagggc gaagttatcc ctcaccctaa acgcataaat gcctgatgcg
    17941 ctacgcttat caggcctaca taactcctgc aatattttgg atttgcatga tttgtaggcc
    18001 ggataaggcg ttcacgccgc atccggtatg aacaacgcgc acttagtcaa taatcaggat
    18061 gtgaggttac ccctcccctg tcatatccgt aaaaacggca tacagaatat cacatcagcc
    18121 gctaaagacg ttacctgcgc ccggagccgt tttcagcacc caaacccgtc catagtggtt
    18181 gtaccaacct gcgcggtgtc cggcttccgg cccgatccct tgatagatat cgaagtgttg
    18241 gcctttgatt gcgcccccga catccaacgc caccatcaga cgcaattcgt actgaccgtt
    18301 aaatttgccg ttattatcca gcagcggcac ttctgccagc aaggtagtac ctggcggaat
    18361 aattgaacga tcggaggcaa cagaagcacg gccaaccagc ggcaccgcac ttgccccttt
    18421 caccggtgca aaagattgcg gtttaaagaa gacgaaagac gggttctgtt ccagcagctc
    18481 gcgaacctcg gcttcactgt gtgtttcgcc ccaatgacga atcgcctgca tcgacatatc
    18541 ttcttttttc acttcgccac ggtcgatcag caccttacca atgctgcgat aggcatgacc
    18601 gtttttccct gcatagctga aaaagttaag cggactgcca tcaccaaagt cgatataccc
    18661 actaccctgc acatccataa tgaagttatc catcagagag ttgctgtaag cgaggatgta
    18721 tttatcgccc aatgccccgg cgtagatctc cgcacgagac ggcagacgac cacgttttgg
    18781 cggcatacgg taaataggat actggaactc gccctggcgg gtatggcgcg cctgaattac
    18841 cggcgtgtaa tagcctgtaa actgcacgtt accatagttg tcggcacctt ccatctgcca
    18901 ggcatcaatg ccgaactggc gcatattgcg ggtatcaccg cctgcgcgca gccactcttg
    18961 caccgcgtta taaacattac tctggttgcc atacagacgc ggtgacgaat tacggatatg
    19021 gttaatttgc tcggcaaaat caccggcgtt aatcggcgcg ccaacggcat ctggctggtt
    19081 caccagagag aaaggctggg taaatttccc gtctttatat tgctgtccgc gatcggttgg
    19141 tttggaagag caggcggcaa gcattgccac aaccgtgccc ataagaaggt actttaccca
    19201 acgtcctttc attgttctct tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac
    19261 cgccgcgttt aatgaaatga gcagcctctc cctgacgcaa attttgcaca aaaaataggc
    19321 tttagtggtt tgtttttgtt caaaatcatg ctaaatccgt gatcggggta aaaaaaaggt
    19381 tgcatgaaaa cgcgagcgga gtatagtgcg catccacgga cgcggggtgg agcagcctgg
    19441 tagctcgtcg ggctcataac ccgaaggtcg tcggttcaaa tccggccccc gcaaccaatt
    19501 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaaa gcgccattca
    19561 gcgccttttt atcatcccct tctcgccagc gttgccccat cggcaaaata cgctttaatc
    19621 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
    19681 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
    19741 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat ttttccgagc
    19801 ttattcagta ccgctttacc aaacttaagg ctgtcagcaa tagtcagcga ctgcaccata
    19861 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
    19921 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag
    19981 agcgcaaaca ccgaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag
    20041 acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag
    20101 atgtcttcat tgcgcgtcat gtacaccttc atattgccct cttcctcgat cagagagcgc
    20161 aaacggcgag ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca
    20221 ccggagtctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
    20281 gctttacccg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
    20341 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
    20401 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
    20461 tttacgtttt gctttaattc aaaaaccatg cgtacggttt gcgggtcaaa ttgcccgacg
    20521 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
    20581 gagttcaggt ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac
    20641 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
    20701 cagacgcgca ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat
    20761 agccacatag cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
    20821 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
    20881 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
    20941 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
    21001 cgaaaaaaca gaataaaaat acaataattt caaataatca tgcaaagggg tgtgccgtgg
    21061 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
    21121 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
    21181 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
    21241 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
    21301 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
    21361 gaacattgca actggatatt actgctcgcc tgtcaatgag tctcaataac acgccgctgc
    21421 agggcgcgca tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg
    21481 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgctcc
    21541 atcgtcaact ggaaagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
    21601 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
    21661 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
    21721 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
    21781 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
    21841 tgcgccgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
    21901 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
    21961 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
    22021 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
    22081 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
    22141 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
    22201 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
    22261 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
    22321 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
    22381 tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
    22441 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
    22501 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac gacgacgcgc
    22561 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
    22621 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
    22681 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
    22741 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
    22801 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
    22861 cggatgacga tgaattttgc gcttctgttg cataaactgt tcaattcgct cattcagtcc
    22921 agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
    22981 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
    23041 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
    23101 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
    23161 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
    23221 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
    23281 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
    23341 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
    23401 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
    23461 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
    23521 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
    23581 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
    23641 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
    23701 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
    23761 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
    23821 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
    23881 aacctcaatg gtatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
    23941 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct
    24001 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
    24061 gacacaggtc gccaacagcg gattcgacgc ctcgttattt tccagtcgtg aaagcggcaa
    24121 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
    24181 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
    24241 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
    24301 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
    24361 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
    24421 agatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa
    24481 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
    24541 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
    24601 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
    24661 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
    24721 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
    24781 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
    24841 cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
    24901 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
    24961 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
    25021 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
    25081 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
    25141 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
    25201 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
    25261 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
    25321 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
    25381 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
    25441 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
    25501 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
    25561 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
    25621 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
    25681 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
    25741 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
    25801 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
    25861 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
    25921 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
    25981 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
    26041 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
    26101 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
    26161 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
    26221 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
    26281 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
    26341 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
    26401 atttcgcgaa acatgaacgt gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
    26461 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcatgaac ttcactacgc
    26521 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
    26581 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
    26641 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
    26701 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
    26761 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
    26821 gccaatgcgc gggtgatcaa cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
    26881 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
    26941 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
    27001 gcccaggcgc tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga
    27061 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
    27121 tttaccgtat caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
    27181 ggcggcggtg ctttgataac cggcggttcg ccttgcagat aacgattaat atcgcgcagc
    27241 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
    27301 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
    27361 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
    27421 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
    27481 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg
    27541 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
    27601 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc
    27661 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
    27721 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
    27781 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
    27841 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
    27901 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
    27961 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
    28021 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
    28081 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
    28141 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
    28201 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
    28261 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
    28321 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
    28381 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
    28441 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
    28501 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
    28561 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
    28621 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
    28681 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
    28741 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
    28801 ctgctcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
    28861 cagacgctgg gtgtaaccat tagcattaat cataaggccg ttgttagcgt tggtggaaaa
    28921 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
    28981 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
    29041 aatcaggctg acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac
    29101 gcgcagattc gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat
    29161 cagcgagaaa tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
    29221 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
    29281 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
    29341 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
    29401 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
    29461 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    29521 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    29581 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcagtta aagacgcaga
    29641 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    29701 aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag
    29761 ataggtaatc aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    29821 ctcaacgcgc aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg
    29881 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    29941 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    30001 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    30061 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    30121 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    30181 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    30241 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    30301 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    30361 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    30421 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    30481 gggcactacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    30541 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    30601 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac
    30661 aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    30721 ttattttctg gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    30781 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    30841 taagcagata gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg
    30901 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    30961 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    31021 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    31081 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    31141 atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    31201 gttcggctgc aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt
    31261 cgccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    31321 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    31381 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    31441 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    31501 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    31561 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    31621 gtccttccag aataaatggc tcggtgtctg ggatttcgct gtcttcagta tggaagttca
    31681 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    31741 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    31801 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    31861 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    31921 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    31981 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    32041 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    32101 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    32161 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    32221 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    32281 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    32341 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    32401 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    32461 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    32521 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    32581 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    32641 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    32701 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    32761 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    32821 ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga
    32881 taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca
    32941 ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga
    33001 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg
    33061 ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg
    33121 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    33181 tgttcacacc cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    33241 gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag
    33301 aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc gagttgccga
    33361 catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg
    33421 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag
    33481 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    33541 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    33601 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    33661 cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat
    33721 attccaccag cgccttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    33781 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    33841 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    33901 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    33961 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    34021 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    34081 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    34141 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    34201 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    34261 gacaattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    34321 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    34381 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agcttttggt
    34441 actggtttct gctggcgaga ctgttcattg atgttcgctg gatacccgat aacgcagtga
    34501 caatcatcac catcagcacc atcagcacca tcagcaccat cagcaccatc agcaccatcg
    34561 caaccattac ctccggcagg ctaaagcctt gttgattctt cagggaagct gacataatgc
    34621 cccctctttc agcggacaaa aatcgctcca gccgcgtggc gaaaaaacaa tttttccatc
    34681 aatgacttcg cctgttcgcc acaacgaaac gccttcataa ccggcaatca ataaggcttc
    34741 attatctgcc agtaaacgca aacaaacctg ggcatcggtt ccagcgtact gcgagcactg
    34801 aactgctggc tgcgtctgcc aggagtgcat ttttccccac gccagcgccg actgaacgat
    34861 ggcctggcgg cgcaatgact gactttccat gctcacgcga gaggcaaaac tgcgatcctg
    34921 ctgactcatt ccttgcaata gcaagctacc caaaaccagc agcatcagga ccagagccag
    34981 tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg tcacgctata gctggcatcc
    35041 accacggttt gcggttcaga cttactggca gcacgcatat taaccgtcaa caccggcgag
    35101 aagccgctga catcctgacg tacgacctga aaagtgtcga taatgatggc atccggatta
    35161 gtgactttat cccagccctt accttcacag gatgtcgcac cgcgtagcgt ttccagcaca
    35221 tgctccttca gacgaaatcc aatctggtcg gactctttta ccggttcgcg atcccagata
    35281 ccgttactgt tcgcatccca ctgcacaatg atacagtcac cctgtccgac aatttccagc
    35341 ccttcgccgg tacagctgcc atgacaataa cccgccctct ggagatgctt cgcgacggta
    35401 aatacccgca gccagatttc atcttccagt gccagcttac gagtgctcgt taaactttca
    35461 cgctgtaacg caggcagaaa gcgtgcagcc cccagcaaca atacgctact gatcgccata
    35521 gcaatcaaca cttccagcag agaaaaacct tgctctttta caggcatcct tctgtttctc
    35581 cttgctgata aagccggagt cttccccacg gcgaaaccac cagccaccac tcgcccgttg
    35641 agtttttgaa gcgaatatgc acggcccatg cggtattgcg caggccaaag aaagcaagcg
    35701 aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg cacaaagacc aatggtgaac
    35761 tgccatgaca ggtattggcc ccagcagcgg aactcacaag gcaccataac gtcccctccc
    35821 tgataacgct gatactgtgg tcgcggttat gccagttggc atcttcacgt aaatagagca
    35881 aatagtcccg cgcctagctg gcggtttgcc aaagccgttg cgactgctgc cagtattgcc
    35941 agccatagag tccacttgcg cttagcatga ccaaaatcag catcgcgacc agcgtttcaa
    36001 tcagcgtata accacgttgt gttttcatgc cggcagtatg gagcgaggag aaaaaaagac
    36061 gagggccagt ttctatttct tcggcgcatc ttccggacta tttacgccgt tgcaggacgt
    36121 tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag ggtaaaaaaa ccgacgcaca
    36181 ctggcgtcgg ctctggcagg atgtttcgta attagatagc caccggcgct ttaacgcccg
    36241 gatgtggatc gtagccttca atctcaaagt cttcgaaacg gtagtcgaag atggattcgg
    36301 gtttacgttt gataatcaac ttcggcagcg gacgcggttc gcggcttaat tgcagatgag
    36361 tttgatccat atggttgctg tacaggtgcg tatcgccacc ggtccagaca aaatcaccca
    36421 cttccagatc gcactgctgc gccatcatat gcaccagtaa cgcgtagctg gcaatgttga
    36481 acggcaggcc gaggaagacg tcacaggagc gctgataaag ctggcaagag agtttgccgt
    36541 ctgccacata gaactggaag aatgcatggc acggtgccag cgccatttta tccagttcgc
    36601 ctacgttcca cgctgaaaca ataatgcggc gcgaatccgg gtcgtttttc agctggttca
    36661 gtaccgtagt aatctggtca atatgacgac catctggcgt tggccaggcg cgccactgtt
    36721 taccatacac tggcccgagg tcgccatttt catcggccca ttcgtcccag atggtgacat
    36781 tgttttcgtg tagataagca atgttagtat cgccctgcag gaaccacagc agttcgtgga
    36841 tgatggaacg caggtggcaa cgtttggttg tcaccagcgg gaatccatcc tgcaggttaa
    36901 aacgcatctg atgaccaaaa atggaaagcg ttccggttcc ggtacggtcg tttttctgtg
    36961 tgccttcgtc gagcactttt tgcatcagtt ctaaatactg tttcatggtt cctcaggaaa
    37021 cgtgttgctg tgggctgcga cgatatgccc agaccatcat gatcacaccc gcgacaatca
    37081 tcgggatgga aagaatttgc cccatgctga tgtactgcac ccaggcaccg gtaaactgcg
    37141 cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc gccgtagcca atcaggaaca
    37201 agcctgagac agctcccatt gggcgcggtt tacgaatata caggttgagg ataataaaca
    37261 gcaccacacc ttccagcagc agttcgtaaa gctgtgatgg gtggcgcggc agcacaccgt
    37321 aagtgtcgaa aatggattgc cactgcgggt tggtttgcag cagcaaaata tcttctgtac
    37381 gggagcctgg gaacagcatg gcaaacggga agttcgggtc aacgcggccc cacaattcac
    37441 cgttaataaa gttgcccaga cgcccggcac caagaccaaa cggaatgagt ggtgcgataa
    37501 aatcagagac ctggaagaag gaacgtttag tacggcgggc gaagataatc atcaccacga
    37561 taacgccaat caggccgccg tggaaagaca tgccgccgtc ccagacacgg aacagataca
    37621 gcggatcggc cataaactgc gggaaattgt agaacagaac ataaccaata cgtcccccga
    37681 ggaagacgcc gaggaagccc gcatagagta agttttcaac ttcatttttg gtccagccac
    37741 tgcccggacg attcgcccga cgtgttgcca gccacattgc aaaaatgaac cccaccagat
    37801 acatcaggcc gtaccagtga agcgccacgg gtcctattga gaaaatgacc ggatcaaact
    37861 ccggaaaatg cagatagcta ctggtcatct gtcaccacaa gttcttgtta tttcgctgaa
    37921 agagaacagc gattgaaatg cgcgccgcag gtttcaggcg ctccaaaggt gcgaataata
    37981 gcacaagggg acctggctgg ttgccggata ccgttaaaag atatgtatat gatccgcgct
    38041 ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc cataaaggct gcaacctgat
    38101 gacgaacttc ggtcgccagt tgcgcttcca gactacgctg cgcaagattt tctgcttcgg
    38161 caaaatcaat gcgccgcagc aggtatttta cccgcgctac agaacgtccg ttcatagaca
    38221 gatggcgata cccaagccca atgaggattg ccacgcacat gggatcgccc gccatttcac
    38281 cgcacaaacg aagatcgata ccatgtattt ccgcttcccg ggcgatcatc gccagagctc
    38341 gtaacattgc aggatgaaga ctgtcgtaaa tgttcgccac ccgggtattg ttgcgatcaa
    38401 cggccagaat gtattgagtc agatcgttgg tgccaacaga gatgaaatcg accctctttg
    38461 ccagatgcgg cagcataaat accattgacg gcacttccag catgatgccg atacgtggtt
    38521 tgggaatttc gtaaccgatc atctcctcga cttcacgtcc ggcacgttca atcaggcggc
    38581 gtgcttcgtc aacttcatcg aggctggtga ccatcggcaa cagaatattc aggttgcccg
    38641 tagcggcatt agcacgcagc atcgcccgca cctggatcaa gaagatctcc ggctgatcga
    38701 gcgtaatgcg aatcccacgc caacccaggc atggattctc ttcgctgatc ggcatgtaag
    38761 gcagctgctt atctgctccg acatccagcg tacgcaaggt gacgggttta tcattgaaca
    38821 tttgcagcat cccctgatac tgcgccacct gttcttcttc cgacgggaaa ccactttgca
    38881 gcatgaatgg gatttcagtg cgataaagac cgatgccatc aatacagctg cccagttttt
    38941 cttcatgttc cgggcttaaa ccagcattga gcatgacttt tatacgctca ccgcttttta
    39001 actgcgcggg taaattgacg tcatcttccg ccagacggct aagctcaatc tcttcactaa
    39061 ttagccgctg atattcttgc agcagtaccg gctccggatc gaccagcaat tcaccgcgat
    39121 agccatcaac gatcagcgtc ctacgatgca gcaccgaagg ctgaatatcc gcgcccatca
    39181 cggtagggat ccccagcgca cgtaccatga tcgcagcatg ggagttggct gcgccatctc
    39241 gcacgacaac accgactaag cgatcctggg gcagctcagc aagagttgtc gctgacagtt
    39301 catctgccac cagaatgaaa cgttccggcc aggcgttcgg cccttgatta gcgtcatcaa
    39361 gatgaaacag caatcgctga cccagcgcac gtaaatcacc agcccgctct ttgagatagt
    39421 tatcgcttag cgcggcaaac tgttcggcaa atttttcaat gaccgttttt accgcccact
    39481 ctgccaccga gcctttatca acctcggcaa acaattcgcg acgcagccgg gtgtccgaaa
    39541 gcaggtgcga gtaaagatcg aaaatagccg ccgtttcttt ttgtgctccg gcggcaaagc
    39601 gtttgctgta gcggcgaaac tcgtttgccg cttcttccag cgccccggtc agtcgttcgc
    39661 gttccagagc cggatccagc gttgatgcct gatacacctg ttccattaaa ggtaacgtgg
    39721 catcctgcca gccttcggca atcgccacac caggtgctgc cggtaatgcg cggattcgcg
    39781 tctggcgata ttgcccaaac agggcagtca actgcgactg agaaagaata gctgccatct
    39841 gggtggcaag cgtcaccagg aaggattctt cactttcgtc atactggcgc aactctcgtt
    39901 gctgtaccac cagtacacca agcaactggc gacgttgaat aattggtacg cctaaaaacg
    39961 cgcggaaacg ttcttctttt acggagggga tgtatttgaa gctggggtgc ttttgcgcat
    40021 ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc gacgatccct tcatcaaacg
    40081 cgagcgttac agtgcgaccg cgtggttttt tcagcccccg ggtcgccatc aggtagtaac
    40141 aacgtcgatc atgatcggcc aggtagaccg aacagacctc ggtatccatc gcaagacaga
    40201 tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg tgcgctggct accttttcga
    40261 ctatttcgcg caggcgagtg agcataattg gcgtgactta acctctttta cgtcgataag
    40321 cagatgcgtt ttgtggtttt ggcgtatttt cctgcagtga catcaccaca ctcgcgaact
    40381 ctttcattac cctacggtag acatcacgtt taaatgacac cacctgtctg accggatacc
    40441 agtaacttac ccatcgccag ccgtcaaact ctggtgtact gctggtttgc atattgattt
    40501 ctgcatcgcc gcttaccagc tgcaagagaa accatttttg tttttggccg atacaaaccg
    40561 gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta gcgcaaccag ttacgcgttg
    40621 aagcaaggat tcgaacgtct ttgcggctta atcctacttc ttcaaacaat tcacggtaca
    40681 tcgcctgctc tgcggattct ccggggttga ttccgccttg cggaaattgc caggagtgct
    40741 gaccaaatcg ccgggcccac attacctgcc cctggcgatt acaaatcacg atacctacgt
    40801 ttgggcggta gccatcgtca tcaatcaccg gactacctca aaataaagct ttatatacga
    40861 atgattgttt catactccag gaagacggta aaccactctc tgcagggcat tacacactaa
    40921 taacaattga ataactcaca gttatgtgca gagttataaa cagaggaagg ggtggatagc
    40981 cgtttttatt caccttttct gtggatagag ttgtgaagaa ctacggaatt actacgggaa
    41041 aacccggagc attctgaata agcccgatat agacatttta aaatattcat tattagtcaa
    41101 tgcattacat gtaatttgag taatgaaaaa gagtaattcg tgacccaggt cacacctctc
    41161 atttacgggt tggcgaaaga tcaaccaatg ccgtgtttat ccacagaatg tgccactaag
    41221 ttaagcactg aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt aaatgaaaac
    41281 agtagtggag gtttttcaca gttatcccag ctttctgtgg ataacatggt gtaagatcct
    41341 gtttattttc agtgaccaga tttggaaaac ctattgcagt gttgcgcaac tcgattaccg
    41401 gcagcctaaa aagcaatata aatcagagaa ttgaacaaca catgtggaaa aagttacact
    41461 gcgaatattc ggcacataat tgctgtttgt tttttaacca aggtatcatg acatgtccca
    41521 acctcgccca ctgctctctc ctcccgaaac tgaagaacag ttgttagcgc aagcacagca
    41581 actttctggt tatacattgg gagaactggc ggcacttgac gggctggtta cgccagagaa
    41641 tttaaaacgc gataagggct ggattggcgt gttactggag atctggctag gtgccagcgc
    41701 agggagtaaa cctgagcaag attttgctgc tctgggcgtg gaacttaaaa ctatccctgt
    41761 ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt gttgcccggt taacgggtaa
    41821 tagcggggtg acctgggaaa ccagccatgt gcgccacaag ctcaaacgcg tactgtggat
    41881 accggttgaa ggcgagcgca gcatcccgct ggcgcagcgt cgcgtaggat caccgttgct
    41941 gtggagcccg aatgaagagg aagaccgaca gctacgcgaa gactgggaag aattaatgga
    42001 tatgattgtt ctcggtcagg ttgagcggat caccgctcgt cacggggaat atttacagat
    42061 aagaccgaaa gcagcgaatg cgaaagcgct taccgaagcc attggtgccc ggggcgaacg
    42121 gattctgacg ctgccacgcg gcttttattt gaagaagaat ttcaccagtg cactactggc
    42181 ccgtcatttt ctgatccagt agccatcgct ttgacctgcc gctttccggg catataatta
    42241 ccgcttcatt tttttggcag ggctttttag atgttatttg catggataac cgatcctaac
    42301 gcttggcttg cgctcggtac gctgacgctg ctggagatcg ttcttgggat cgacaatatt
    42361 attttccttt ctctggtggt ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt
    42421 ctggggttgg cgggagccat ggttatgcgt ctggcgctgc tggcatcaat ctcttgggtt
    42481 acgcgcctga caaatccgct ttttacaata ttcagtcagg aaatttctgc ccgtgatttg
    42541 attctgcttc tgggtggctt gttccttatc tggaaagcca gcaaggaaat ccacgaatcc
    42601 attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg
    42661 cagattatgc tgctggatat tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg
    42721 tcagatcacc tgtttattat gatggcagcc gtggtgattg ccgtaggcgt gatgatgttc
    42781 gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat gctggcgctc
    42841 tctttcctga ttctggtggg ctttaccctg attctggaaa gtttcgacat ccacgtaccg
    42901 aaaggttaca tctacttcgc gatgttcttc tctattgcgg ttgaaagcct caacttgatt
    42961 cgcaacaaaa agaatccgct ctgatactcc gtacgctctc ctgcgggagg gcgtactcac
    43021 tccttttacc ctcacagttt aagattttcc tgctttcaag atatataacg tcggtttata
    43081 aacagactat tatcataggt aggccaaatg aggataaccg gatgaaaaaa tgggcagtaa
    43141 taatttctgc agtcggactg gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg
    43201 cgaccaaaga tggccgtatg attttgaccg atggaaaacc tgaaattgat gatgataccg
    43261 ggctggtgag ttatcacgat cagcaaggta acgcgatgca aattaaccgt gatgatgttt
    43321 cgcaaattat tgaacgttaa caaataaggt cagcatccgg ctggccttaa gatttttctc
    43381 tttccctttt cccttccctc tgccattttt atattcctta tgtcgtgatt ataaaaagga
    43441 aacggctatg caatatcacc gtatacccca cagttcgctg gaagtcagca cgctggggct
    43501 tggcacgatg acgtttggtg aacagaacag cgaagccgac gcccacgcac aactcgacta
    43561 tgccgtcgct cagggcatta accttatcga cgttgccgaa atgtacccag tacctccgcg
    43621 ccccgaaacg caagggttaa ccgaaaccta cgtcggcaac tggctggcga aacatggcag
    43681 ccgcgaaaag ttaattatcg cctccaaagt gagcggaccg tcgcgcaata atgacaaggg
    43741 catccgcccg gatcaggcgc tggatcggaa gaatatccgc gaagcgctgc atgacagcct
    43801 caagcgccta cagactgatt acctcgatct ttatcaggtg cactggccgc agcgcccgac
    43861 caactgcttc ggcaaactcg gttatagctg gacggattct gcgcctgcgg tttcgctgct
    43921 ggatacgctg gacgcactgg cagagtacca acgcgcggga aaaattcgtt atatcggcgt
    43981 gtcgaacgaa actgcatttg gcgtaatgcg ctacctgcat ctggcggaca aacacgatct
    44041 gccgcgtatt gtcaccattc agaaccctta cagtctgtta aaccgcagtt ttgaagtagg
    44101 tctggcagaa gtcagccagt atgaaggggt cgaactgctg gcctattcgt gcctgggttt
    44161 cggcacgctg accgggaaat atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct
    44221 ctttagtcgg ttcacccgct atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt
    44281 tgatatcgcc agacgtcatg gccttgatcc tgctcagatg gcgctcgcgt ttgtacgccg
    44341 tcaaccgttt gttgccagca ctctgctggg cgcaaccacg atggatcagc tgaaaactaa
    44401 catcgaaagt ttgcatctgg agttaagcga agacgtatta gctgaaattg aagcggtaca
    44461 tcaggtttat acttatcctg caccataaag attaacgccg gttaaaaccg gcgttaaata
    44521 ttaatgacgg cgctgccaga tccacagcgc cgttattgcc agcgcaaata gcgcaccgaa
    44581 gccaatgcca atgggcacga ccgggatgcc taccattacc gccagcgagt aaatgcccag
    44641 catcaacaac atggcgctgt tttcgccaag gttttgtact gcaatcgcat tccccgcccc
    44701 gacgcttttt ttaccccgct cctgtagcaa cgcattgagc ggaacgacaa aaaagccccc
    44761 cagcacgcca atcagcatca acaaggcata ggctggcagc agctcgtgtt gcagggagaa
    44821 aatcggtacc accacgccaa tcaaaatccc ggctggcata cagcgtgaca cggtttccag
    44881 cgtaaccagc ttcgctgccg cacctgcgcc aaccacgata ccaatcgcta ccatcgcgtt
    44941 gagataggtc ggcgtagcgt tatcggtaat gcccagcgcc accggtaccc acagcaccaa
    45001 caggaaacgc agcgtgacac ccgctcccca gaataaactg gtgcccacca gcgaaaaacg
    45061 cgtttcacca ttgcaccata gcgaggtgca ggcattcagg aaactgcggg tcatgttgat
    45121 gagattccag gactgccccg gacgcgccgc cagtttggga atgtagatat tggcaacgac
    45181 cgcaccaccg taggccagtg cgcatgcggc cagagcgacc aggacatgcc agtcagccag
    45241 cacaccaccg gctacggaac cgagtaaaat cgccgctatg gcagaagctt ccattaaacc
    45301 gttagctttt actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg
    45361 tgaataggct gcagcaccaa cacccaccag cgtatagccg agaaacggat tgataccaaa
    45421 gcagatactg gctgcgccca gcagcttcag gccgttggca aacatcatca cccggccttt
    45481 ggcgaagcta tccgccacct gcccgacaaa cggcgccaaa agaatgtaag cacctacaaa
    45541 caccatttgc aggatgggct ggctccactc cggatagaac tgcgctttca gtaacgccag
    45601 agtggcaaac agtagcgcgt tatcgccaaa cgcagagaga aactgcgcca cgataaccgc
    45661 tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt
    45721 ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg
    45781 gcatctgttt cagatagcga atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc
    45841 gggcatactg ttgcaactta tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg
    45901 cctcgccttt gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac
    45961 caagtgccag ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc
    46021 gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag
    46081 tgtcatacca gccgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta
    46141 cacctggctt ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc
    46201 gtccgccctc ttcgatacca gggaccgaca acaggcgtgc atccattcct ggtagaatac
    46261 gccctaccgt accgggtttc gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt
    46321 cggtcacacc gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttgg
    46381 tactttcttg tagtttttct gcgcctgcca ccacatagcg tagacgatag aagtcatacg
    46441 ggttggcgaa acgcgcgtag tgaccgagga aagtcgaggt accgaacaac acggtgcaac
    46501 tgcggtcata caccaagtcc ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca
    46561 cttctgcacc tgtaagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca
    46621 gcggtaatgc cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct
    46681 cgacgttcgc cagaatgctt ttatggctat ggacgacgcc ttttggatgg ccttcagaac
    46741 cggaggtaaa gaggatcagc gcctcttctt ccggctgctg ttttacctgc gccaggcgcg
    46801 gcatcagcaa atgagcgaag atccatactt tgtcggcagt ggtgacatct gcttttaaat
    46861 cttccagata gacccagcgc acctgagtaa gttgctccgg cagatgccag agtttgcctt
    46921 tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc agccgccgta atagcactgg
    46981 tcagcccttt taccccggca gtgtagttca tcattgcggg aatgcggcga cgcgcgatgg
    47041 ccccaaaaat cactgccgca ctgatgcctg cattgggcag cattaagccg atgcgttcgc
    47101 cttcaacact gtatttttca aggatgcgtc caacaaacag cgttttcgta agcaatttgc
    47161 gataggagtc tggggtaaag ttgacgtctt cgacacattt cttcccggct ccgaagcggt
    47221 acattgcact cagtaaagat tcgtacagag tttcacgcgg gcgcaccgcc atacgcgctt
    47281 ccatcattat ttgatgcagc atttcgccag cgattttgcg acggtcacgg gcacgcggcg
    47341 catccggcat cgccacctgc gttggaggca aaatatgcag agtaatttgc gggaacaagc
    47401 gacgtttaac cagacctttc aggcggctga agtgcgtaag ttccgcccct tcaatacgca
    47461 caggaataac cgttgcacca gacttcgccg cgacaaaacc cgcgccatcg tagattttca
    47521 tcagcgagcc tgtagtggtg atgcgtcctt cagggaaaat caccactggt cggccctgtt
    47581 ccaccagacg taccagatgt ttaatagcca taggttgcgt cgggtcgaga ggaacaaagt
    47641 cgataaatga tttcagccaa cgcatatacc actgttggct tattgaggtg taaacggcaa
    47701 acactggacg cacaggtaaa aacagtccaa gcaaaatgcc atcaataaaa gagacgtgat
    47761 taggcgtaat tagtacgcgc tcgcccttca gtgcctgggt gtcacccgta acgcgaacgc
    47821 gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag cattccaact ccctttgctc
    47881 tgattcagta aaagcgaatg gagggagatt acacgagata aagaacgcga gcgacagtaa
    47941 attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa
    48001 gagcgtaccg aataatctca ccaatcaata cctctgggat cttgattgtg gtctgcacga
    48061 cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa
    48121 ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc
    48181 tggtgtaatt catgtgctca acacgaagtt gacttcacct atcaatacct ctgggatcac
    48241 cactttagca acctgaagcc aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag
    48301 tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg gctcacattc
    48361 ccacgatgaa aacacgccac cccttgaacc aacgggcgtt ttccgtaaca ctgaaacaat
    48421 gtaagcgttt acccactaag gtattttcat ggcgaccata aaggatgtag cccgactggc
    48481 aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacccaaag ccagcgaagc
    48541 ttcccggctg gctgtgcata gtgcaatgga gtctcttagc tatcacccga acgccaacgc
    48601 ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg atcgttggtg atgtttccga
    48661 tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca ccggtaattt
    48721 cttattgatt ggcaacggtt accacaacga acaaaaagag cgtcaggcta ttgagcaact
    48781 gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa atgatcccag atgccgattt
    48841 agcctcatta atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt
    48901 tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc tggctggcaa cgcgtcattt
    48961 aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta tttctgacgc
    49021 cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattc cggccaatga
    49081 tcggctggtg acatttggcg aaccagacga aagcggcggc gaacaggcaa tgaccgagct
    49141 tttgggacga ggaagaaatt tcactgcggt agcctgttat aacgattcaa tggcggcggg
    49201 cgcgatgggc gttctcaatg ataatggtat tgatgtaccg ggtgagattt cgttaattgg
    49261 ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc
    49321 aatcgtaacg atggcgaccc aggctgccga actggctttg gcactggcgg ataatcgccc
    49381 tctcccggaa attactaatg tatttagtcc gacgctggta cgtcgtcatt cagtgtcaac
    49441 tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa agcaattcca
    49501 gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa
    49561 acagaacttc tggtaacagc ggacggctat tgtagttgga tgacattgat gcgccatatg
    49621 cccctgtatc atgcagtacc agataatcac ctgccttcac ttccggcaag gcgcgggttt
    49681 caacatttcc cccttcctgc tgggtaaaga catcgcccga ttcacataac ggcccggcga
    49741 cgacggtttc caccgttggc gcgtgttcca gagaacgacc atcagctgcc agggcactga
    49801 tatggtggta actaccgtac attgccgggc gcatcagatc gttgaacccg gcatcaacca
    49861 gcacaaagtg gcggctaccc atttgtttga cgctccgcac ctgagtaatt aatacgccag
    49921 actgcgctac caggaagcga cccggttcaa tttccagttt cacagggtgg cccaaatggc
    49981 gagcgatttg ctcacgcgcg gcattccaca gaccataata atgttcggta tcaaccgcct
    50041 cttcaccctg ttgataagga atagaaagcc caccgcccgc agaaatagcc tgtaaatcct
    50101 gaccgaattc gataacctga cgcaccatag caccacacac ctgctccaga tgggcataat
    50161 caacgccaga accaatgtgc atgtgaatgc cgactagctg cagatgatga cgttgtatca
    50221 cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg cttgctgttt tcgccaccgg
    50281 tattggtttt ttggctatgt ccgtgaccaa accccggatt aacgcgcagc catacccgat
    50341 gccctggcga aacctggccc agttggtcga gcgtatcaac agaacccgca ttcaccggaa
    50401 tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa
    50461 tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa cgcacgctct atttcgccta
    50521 acgagacgga atccactttc acgccctgct cacgcattaa gcgcaaaata tgaatattgg
    50581 aacaggcttt ctgtgcaaag cgcaccactt caaactgttt cagcgctgca atctgccgac
    50641 gaataatttg cgcatcgtag acccacaccg ggcagccaaa ttcagcgggc aaacgcagca
    50701 gattttcggc ggtgagatcg gtatcggtgc tgaacagtga atgtggcata acaaactcca
    50761 gatgagtgct tttttatgat tacgccacat cataaaaaga ataaaaaata tcgatttatg
    50821 tcgagtctat gcaaaaatga tatggattac cggattgcga gagagcgcta atggccgccg
    50881 ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc ctgactgagg
    50941 cagcacacct gctacacacc tcacagccaa ccgtcagccg cgaactggcg cgctttgaga
    51001 aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg attacatcct accgtgcaag
    51061 gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc attgtcagcg
    51121 ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc ctgccggtct
    51181 tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggcacgttat cccgatgtca
    51241 gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc
    51301 atgatttagg actcactgag acactccata cgcctacggg aacagaacgt accgaattac
    51361 tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc gctggcggta aaaaaggtat
    51421 taacgccgga tgattttcag ggtgagaatt acatcagcct ttcccgtact gacagctatc
    51481 gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg atcgtagaaa
    51541 cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggtatt tcggtggtta
    51601 acccgctcac cgcactagat tatgcggcaa gcggtttagt ggtgcggcgg ttcagtattg
    51661 cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca gcgctggttc
    51721 aggcgtttag tgggcattta caagcggggt taccgaaact ggtcacttct cttgacgcta
    51781 ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga tcgccgcggt
    51841 atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc
    51901 aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg cataataagc
    51961 gcgtgacgct tcggtaaatt gccccagaca cagttcttca aaaataatct gattaatttt
    52021 cgcccgttca tccgcttcag gaataagaca attgatggaa aattgttccg tcagccgccc
    52081 gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc agcgccacac gagtcattcc
    52141 ggccccggta attgcacgtc cggtggcatc cgcaatgtgt aagaaaggca gagtgcaacg
    52201 tgactcaatg gcatccgcca ctttatgcat cgtattggta cataacacaa taccttctgc
    52261 gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg tccccggttt tatcccattc
    52321 cccgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca gcatttgcgc
    52381 agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcagacgat agtaaggaat
    52441 ggtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc tctcctgatg
    52501 tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc
    52561 aatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg aaatcagacg
    52621 ccgatatttc tcaacttctc gcctgccatc agtttgcgtt cgatatgttc cagcgtgaca
    52681 tttttggttt ccggaatgag ccagaaagta atacccacaa acgcaatgtt cagcgcagtg
    52741 tagagccaga acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa ggccgcgccg
    52801 ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg taataccgaa atcgcggcat
    52861 ttcagcggct gaatttcaga gcacaggatc cacaccactg gcgcggcgct catcgcataa
    52921 ccggcaatac acatcatcgt catgccaaca gagagccagg acaagccact ggaagccgta
    52981 ccgttatcaa actgcatcag gcaatagccc agcaccagag tgcctaacgc catcacgctg
    53041 aaaccaattt tcagagccgg tttacgacct gctttatcta ccgtaaagac cgcaatgaag
    53101 gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa tcatctgttg ttctgtggtc
    53161 gtaaagcccg ccattttgaa gatacgcggc gcgtagtaca tgatgatgtt cataccggta
    53221 aactgctgca tcgcctgcaa caacataccg agaaacacag cacgacggac gttacggttg
    53281 atcttaaaca gtgcccaacc gccctgtttt aacttcaggc tttcacgaat ttcgttgagt
    53341 tcttctcgcg ctttttccga cgtatcgcgc agcatacgca acacttcttc cgcctcaata
    53401 tgacgcccct tttccgccag ccagcgcggg ctatttggca ggaagaccac cagaataatc
    53461 agcagaactg ctggtaaagc aagaaccccc aacattgcgc gccagttacc gctataactg
    53521 aacgctgtat cggataaaaa cgccagcacg atgccgagtg tgaccatcaa ctggtacata
    53581 ctgatcatct taccgcgaac gttttcactt gccatttcag aaagatacag aggagcggtg
    53641 taagacgcga tcccgacagc aatgcccagc accacacgag cggcgattaa catctctacg
    53701 ctggtcgcaa aagcggaccc tatagaaccg agtacaaaca ggatggcccc cgccatcagg
    53761 ctgtatttac gccccaggcg gaacgacagc caaccattaa acagcgcacc aattgctgcg
    53821 ccgagcatca tgctactaac cacccattcc tgcaaacgac tggtcagcac aaagtgatcg
    53881 gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa caatcctgcg
    53941 accgcagcag ctaccgaaac aaacatattc atacgccgcg tatcacgcaa agaacgtggc
    54001 gttaaagcag attccgtatt gatagtaacc attttttcct gccagcagag agtaagacat
    54061 tgtgaaaaaa tacgtgaaca attcacacag gtgtcaggtc ggaaacagca taaatatgga
    54121 ttaaattgct gcgacatgcc gttatgtgat ggatattcca attttaaaat taagttgaat
    54181 tattgagatt attattaacc tcctaatttt aaagcagata aaattcataa agttcattaa
    54241 ttgataattg atatggatta tttcataacc atgatatgga ttatgatgat ctagagatat
    54301 aaaaaaccct gccatgcggc agggtcataa aagtaagagg aatgaattaa cgcgccagcc
    54361 aaccgccatc cacagcaatg gtataaccat ttacataatc tgaagcgctg gaggcaagaa
    54421 acactaccgg ccccatcagg tcactcggca gtccccaacg gccagctgga atgcgatcga
    54481 gaatttccgc gctacgttgt tcatctgccc gcagttgttg ggtattgttg gtcgccatgt
    54541 aacccggagc tatcgcatta acattaatgt tgtgtttagc ccattcgttt gccatcaatc
    54601 gcgtcacacc catcacgccg ctttttgatg cggtataaga aggcacacgg atcccgccct
    54661 ggaaggagag cattgacgcg atattgataa tcttgccgcc attgccttgc gcgataaagt
    54721 gtttcgccgc tgcctgagac atgaagaata cgctcttgat attcaggttc atgacatcgt
    54781 cccagtcttt ttcgctgaac tcgagagcgt cttcgcggcg aatcaatccg gcgttattca
    54841 ccaggatatc aatatgacca aattcagcta ccgcgcgatc cagcagtcca ggaatgccat
    54901 caatctttcg cagatcggcg gtcaggctta aaaaacgacg ccccagcgcc gtgacctgct
    54961 cgatggtttc agtcggttca acgatgttaa tgccaacaat gtcacagccc gcttgcgcca
    55021 gccccaacgc catcccctgg cccagcccag tatcacaacc agtgacgacc gcaactttac
    55081 cttggagaga aaatgcactt aaaatcataa caacacctta ttcgtttatg cccacaacta
    55141 gcgcaaatct ttaacgccca catggtccat atcattaaag acctggtttt caccgaccat
    55201 gccccagata aaggtataag ctttggttcc gacgccggaa tggatcgacc agctcgggga
    55261 gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt tcttgcggct gccccatcat
    55321 gtggaaaacg caggcgtcat catccatatt gaaatagaaa taaacttcca tccggcgctc
    55381 gtgggtgtga cacggcatgg tgttccacaa gttacctgga gccagctccg tcagccccat
    55441 actcaattga caggtttcca gtacatccgg gacaaaatat ttgttaatcg tgcgacggtt
    55501 actggtgagg ttatcgccta acgtgactgg agatacttcg tccggtgtga cttttttggt
    55561 gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac ttcgccggag tggcggtatc
    55621 aatactggca aagacaactt cttttgcacc tttaccaaca tacagggcgt cgcggtgacc
    55681 gatttcatag cattgaccat cgacagtaat cgtaccggca ccgccaatat tgataacacc
    55741 taactcgcga cgttcaagga aatagcttac gccgagttgt ttaccaactt ccccgccaac
    55801 ggaaaccgtt ttagttatcg gcataatgcc gccaacaata attcggtcaa tgtggctgta
    55861 aaccatggtg tactcatcgg cgacaaatac cttttcaacc aaaaattcat tgcgcagccc
    55921 ttgggtatcc agcgtttttg cgtgcgcacc gtggatgctc tgtcttacgt ccacatcaac
    55981 ctccgataat cagtgaagcg tgaaaataga aaacgatagt gaaacactgt ttcgttttct
    56041 ctataaaggg atgatatgcc catgggctag ggttttcaat taaaaataaa acaatgtttt
    56101 atttttaatt taaggattaa aaaaagtgca gtgtcgatca cgaatatgtc atgagaatgg
    56161 aaaaatgagg gagcccagaa aatgtatgtc gacttgccag ccatcattat tcaacagatt
    56221 aatgacggca gatatttatc ggtaaggagg aaatgcagag ctactcgtca cgttcaatgg
    56281 tcaatgccac gccctggccc ccgccgatac aaagcgttgc cagccctttg cgggcattac
    56341 gtttcaccat ttcatgaacc agagaaacca ggattcggca accggaagcg cctatcgggt
    56401 gaccgagtgc gatcgcgcca ccattgacat tgacccgacg ctcatcccac tcaagcatct
    56461 tgccaaccga aagcgcctgt gcagcaaacg cttcattagc ctcgataaga tcgacatcag
    56521 ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc atacaccggc gcaattccca
    56581 tcaatgccgg atctacacca acgctggcaa atgcgcggat gcgggccagc acgggtaaat
    56641 tcaacgctcg tgctttggct tcgctcatca tcattaccgc agctgcgcca tcgtttatgg
    56701 atgatgcatt ccccgcggtc acagaaccga gactatcaaa tgaaggattt aaacgggcta
    56761 aaccttctgc actggcgtca gtgcgtggct gttcatcggt atcaacaacc aacgtctgac
    56821 cgttactttg gatcattacc gggacgatct catctttaaa tcgtccggcg tcaatcgccg
    56881 ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg caactgacgg ctgatgccat
    56941 attcgcgagc cagattttcg gcggtgacac caatatgata atcattgaag gcatcccaca
    57001 acccatcatg caccagactg tcaaccaact ggctattgcc aagctgttca ccggtgcggc
    57061 tatcagtcag aacatgtggt gcgcgactca tgttttcctg gccacctgcg atgacaatat
    57121 cagcctcgcc acactgtatc gcctgagtag ccagatgcag tgctttaaga ccggaaccgc
    57181 aaacgtcatt aatcgtgatt gcagaaacgc tgttaggaag accaccttta atagccgatt
    57241 gccttgccgg attctgccct gcccctgcag tcaacacctg accaagaatt acttcatcca
    57301 ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt cacgaccaga ctaccaagtt
    57361 ccacggcgga atgacccgct aacgcaccac gaaagcagcc gataggtgtc cgtaacgccc
    57421 cgacaatcac aacgtctttc atcacaacct cgcaccaatt aacagtgcaa tagtaaatga
    57481 ttgttaatta cagttatctt aattgtttaa aaaaagtgat ttttatcaca aaggaaatat
    57541 gcctgagcag tagtcagagt cataactggc acgtaaggtt tgcaaccact aacccaccaa
    57601 tagatgggta gatagggcgt taatctccca tacttaactt ggtttatggt aaattgccct
    57661 ccattttgtt taatttgtag atgatacgtt cagataatgt ctaatatttg gtctaaagaa
    57721 gaaactctgt gaagtttcgc gctctatggc acagccgttg atgcaggcac gctattcctt
    57781 cctattcagt taggttcggc aggggctgtg gtcctgttta ttactgctct ggtcgcctgg
    57841 cctttaacat attggccaca taaagcctta tgccagttca tcctctcatc aaaaacatca
    57901 gcaggtgaag ggataacggg cgcggtaaca cactactatg gcaagaagat tggtaatctg
    57961 attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt tgatatatgc agtggcaatt
    58021 accaactcac ttacggaaca gctggcaaag catatggtta ttgatcttcg catccgtatg
    58081 ttggtgagtc tgggtgttgt attaattctg aatctcattt ttctgatggg acgtcatgcc
    58141 actattcggg taatgggatt tttggtattc ccattgattg cctatttctt atttctttcc
    58201 atttaccttg tcggtagttg gcaacctgat ctattaacaa cccaggtaga gttcaatcag
    58261 aatacccttc accagatatg gatatcgatt cccgtgatgg ttttcgcctt tagccatacg
    58321 cccattattt ctacgtttgc catagacaga cgtgaaaaat atggcgaaca cgctatggat
    58381 aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct gcataagtgt actgttcttt
    58441 gtctttagct gcctgctttc tattccatct tcgtatattg aagcggctaa agaagaaggg
    58501 gtcaccattt tatcggcgct ttctatgctg ccgaacgccc cagcatggtt gtcaatttcc
    58561 gggattattg tcgcagtagt tgcgatgtcg aaatcattcc tgggtacgta ctttggcgtt
    58621 attgaaggtg ccacagaggt cgtcaaaaca acactacagc aggttggtgt aaagaaaagt
    58681 cgtgcattta accacgcact atcaattatg ttggtatcgc tgattacctt cattgtttgt
    58741 tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca gcggcccgct cattgccatg
    58801 atacttttca tcatgcctac gctgtcaacg tatctcatcc cggcgcttaa accctggcgt
    58861 tccatcggaa atctgattac gctgatcgtg ggtatcctgt gcgtatcggt aatgttcttt
    58921 agctaatatc cgttgtggat ggttcaaaat tgctacaatt ttgaaccatt ccatttttca
    58981 taatattcat ttgttattat tccgccctat cacttctctc gtcatatcct agaattatca
    59041 gttagttaaa cacacaaaga gatctgaaca tcttaaataa gactatttaa gatgcataac
    59101 ttagattcgc aagatatctt ctggtcattc aaaaacaatt tgcgatatag atattctcat
    59161 accatgagat agttatctgg accacgttcg accatgcaag attaaatcag tgaaacataa
    59221 tcatatttaa taacgagaaa tgcattttta aatatcttaa tcagaattat agcaaataca
    59281 gattaatcgc aatttttccc agcaaaatta caaaatcaag gtgtattgac atcatcaatc
    59341 accaatgaaa ttatgcaatt atatacggat agggaggttc ttaacatggg gctttgtagt
    59401 cgttataaaa gtcttacatg caatagttgc tcaatgcatt gccaaataat gccagaagag
    59461 tcaccgcgtt tgcagtactg c
//
LOCUS       DAJTYM010000012        58133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTYM010000012 DAJTYM010000000
VERSION     DAJTYM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1101)
                     /locus_tag="OO575_000749"
     CDS             complement(<1..1101)
                     /locus_tag="OO575_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR6663822.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO575_000750"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO575_000750"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6663823.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO575_000751"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO575_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6663824.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO575_000752"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO575_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6663825.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO575_000753"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO575_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6663826.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO575_000754"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO575_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6663827.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="OO575_000755"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="OO575_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6663828.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="OO575_000756"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="OO575_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR6663829.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="OO575_000757"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="OO575_000757"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6663830.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="OO575_000758"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="OO575_000758"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6663831.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKHLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="OO575_000759"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="OO575_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6663832.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="OO575_000760"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="OO575_000760"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6663833.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="OO575_000761"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="OO575_000761"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6663834.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="OO575_000762"
     CDS             11851..12015
                     /locus_tag="OO575_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663835.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="OO575_000763"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="OO575_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6663836.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="OO575_000764"
     CDS             13524..13769
                     /locus_tag="OO575_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6663837.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="OO575_000765"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="OO575_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6663838.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="OO575_000766"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="OO575_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6663839.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="OO575_000767"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="OO575_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6663840.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="OO575_000768"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="OO575_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6663841.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="OO575_000769"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="OO575_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6663842.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="OO575_000770"
     CDS             19432..19785
                     /locus_tag="OO575_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6663843.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="OO575_000771"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="OO575_000771"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6663844.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="OO575_000772"
     CDS             21085..22212
                     /locus_tag="OO575_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6663845.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="OO575_000773"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="OO575_000773"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6663846.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="OO575_000774"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="OO575_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="OO575_000775"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="OO575_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26571..26744
                     /locus_tag="OO575_000776"
     CDS             26571..26744
                     /locus_tag="OO575_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663847.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="OO575_000777"
     CDS             26834..27583
                     /locus_tag="OO575_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6663848.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="OO575_000778"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="OO575_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6663849.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="OO575_000779"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="OO575_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6663850.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="OO575_000780"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="OO575_000780"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6663851.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="OO575_000781"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="OO575_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6663852.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="OO575_000782"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="OO575_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6663853.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="OO575_000783"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="OO575_000783"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6663854.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="OO575_000784"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="OO575_000784"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6663855.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="OO575_000785"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="OO575_000785"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6663856.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /locus_tag="OO575_000786"
     CDS             complement(35512..35607)
                     /locus_tag="OO575_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663857.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /locus_tag="OO575_000787"
     CDS             complement(35721..35888)
                     /locus_tag="OO575_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663858.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36055)
                     /locus_tag="OO575_000788"
     CDS             complement(35891..36055)
                     /locus_tag="OO575_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6663859.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="OO575_000789"
     CDS             complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="OO575_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6663860.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37534..40131)
                     /gene="topA"
                     /locus_tag="OO575_000790"
     CDS             complement(37534..40131)
                     /gene="topA"
                     /locus_tag="OO575_000790"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6663861.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40511..40762
                     /locus_tag="OO575_000791"
     CDS             40511..40762
                     /locus_tag="OO575_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6663862.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="OO575_000792"
     CDS             complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="OO575_000792"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6663863.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42067..42825
                     /locus_tag="OO575_000793"
     CDS             42067..42825
                     /locus_tag="OO575_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6663864.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42822..43412
                     /gene="cobO"
                     /locus_tag="OO575_000794"
     CDS             42822..43412
                     /gene="cobO"
                     /locus_tag="OO575_000794"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6663865.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="OO575_000795"
     CDS             complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="OO575_000795"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6663866.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44425..45045)
                     /locus_tag="OO575_000796"
     CDS             complement(44425..45045)
                     /locus_tag="OO575_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6663867.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45042..45923)
                     /gene="yciV"
                     /locus_tag="OO575_000797"
     CDS             complement(45042..45923)
                     /gene="yciV"
                     /locus_tag="OO575_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6663868.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46061..46105
                     /gene="trpL"
                     /locus_tag="OO575_000798"
     CDS             46061..46105
                     /gene="trpL"
                     /locus_tag="OO575_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6663869.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46082..46175
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46197..47759
                     /gene="trpE"
                     /locus_tag="OO575_000799"
     CDS             46197..47759
                     /gene="trpE"
                     /locus_tag="OO575_000799"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6663870.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47759..49354
                     /gene="trpD"
                     /locus_tag="OO575_000800"
     CDS             47759..49354
                     /gene="trpD"
                     /locus_tag="OO575_000800"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6663871.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49355..50716
                     /gene="trpCF"
                     /locus_tag="OO575_000801"
     CDS             49355..50716
                     /gene="trpCF"
                     /locus_tag="OO575_000801"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6663872.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50728..51921
                     /gene="trpB"
                     /locus_tag="OO575_000802"
     CDS             50728..51921
                     /gene="trpB"
                     /locus_tag="OO575_000802"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6663873.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51921..52727
                     /gene="trpA"
                     /locus_tag="OO575_000803"
     CDS             51921..52727
                     /gene="trpA"
                     /locus_tag="OO575_000803"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6663874.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53107..53286
                     /locus_tag="OO575_000804"
     CDS             53107..53286
                     /locus_tag="OO575_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6663875.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53372..53872
                     /locus_tag="OO575_000805"
     CDS             53372..53872
                     /locus_tag="OO575_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6663876.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53918..54424
                     /locus_tag="OO575_000806"
     CDS             53918..54424
                     /locus_tag="OO575_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6663877.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="OO575_000807"
     CDS             complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="OO575_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6663878.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55362..55451
                     /locus_tag="OO575_000808"
                     /pseudo
     CDS             55362..55451
                     /locus_tag="OO575_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55479..56222
                     /locus_tag="OO575_000809"
     CDS             55479..56222
                     /locus_tag="OO575_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6663879.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56252..56791
                     /locus_tag="OO575_000810"
     CDS             56252..56791
                     /locus_tag="OO575_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6663880.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56896..57294
                     /gene="yciA"
                     /locus_tag="OO575_000811"
     CDS             56896..57294
                     /gene="yciA"
                     /locus_tag="OO575_000811"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6663881.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="OO575_000812"
     CDS             complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="OO575_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6663882.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
BASE COUNT        13947 a        15044 c        14544 g        14598 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca ggtgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tggcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataagcatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata
    36301 tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg
    36361 gcagttttat atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg
    36421 catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac
    36481 tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt
    36541 gagcatcaac gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga
    36601 cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga
    36661 aaacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg
    36721 taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt
    36781 ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg
    36841 gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat
    36901 tgcctttaga gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa
    36961 cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg
    37021 tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg
    37081 attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg
    37141 ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt
    37201 cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg
    37261 ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt
    37321 tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg
    37381 gtgactaata cgatttaata ataaaaaagg tgctaactat aaccactata tcatttatag
    37441 ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg ctttcgcgac
    37501 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
    37561 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
    37621 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
    37681 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
    37741 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaacacacc
    37801 ggcagcaccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
    37861 tggcaccgga tcttctttcg gtggtgccac ttcaccgtta cgtaaaatct tacgtgtgtt
    37921 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
    37981 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
    38041 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
    38101 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
    38161 gcgcagcgcg ttggtttcag catcttcgcc ttccagcacg ttcagcactt cgttttccgg
    38221 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcatagc cagaacagcc
    38281 aaggaatacc ccggtactcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
    38341 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
    38401 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
    38461 ttcgtgattt gccacctggt cgaggctgtt ttccatctgc gcggtaaagt cgtagttcat
    38521 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
    38581 gaaacgacga ttttctactc gcacatagcc acgatcctga atggtcgaaa tgatcgacgc
    38641 ataggtagac ggacgaccga taccacgttt ttccagttct ttaaccagcg atgcttcact
    38701 gaaacgggct ggcggcttgg taaagtgctg ggctggtgtt agttcaacga gcgtcagagc
    38761 atcgccttta tcgactgccg gtaagatacg atcttcatcg cctttacgca gtgcaggcat
    38821 cacttttgtc cagccatcaa agcgcaaaat acgaccgcgt gctttcaggc ggaaatcgcc
    38881 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggcaacgaa
    38941 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
    39001 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
    39061 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
    39121 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
    39181 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaaacc
    39241 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
    39301 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
    39361 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcattct gatgagtcac
    39421 ctgcaactcc aacgcttcac cagatggcgt agtcgtgctg gcatcgactt cccagaactc
    39481 ttccggtacg aacgctttaa tttcacgctc acgctctacc accaggcgga ccgccaccga
    39541 ctgcacacga ccggcagaca gaccacgagc gatctttttc cacagcagcg gcgaaaccat
    39601 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
    39661 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
    39721 cactcggcta tagcgcgcat catcaccacc aatcacttcc cgcaggtgcc atgcaatggc
    39781 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
    39841 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
    39901 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
    39961 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
    40021 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcacaaccca gatacttgtt
    40081 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
    40141 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
    40201 ttaccgatat ggactcgctc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
    40261 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggattttt
    40321 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
    40381 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
    40441 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttgtttaa
    40501 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
    40561 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
    40621 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
    40681 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
    40741 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
    40801 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
    40861 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
    40921 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
    40981 gcctttctct accgcctgtt gtccgtacca gtgttcaccc gttgccacct gttcaatatc
    41041 cagagacgga cgcatacgct tcacaaaatc tttaaataac tgatgcgttt cgttcagctc
    41101 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaaggtca gcgtgcgctt
    41161 atactgcccg gcggtgtgca gttcgatatc aatatctttg cttttcagga agcggttaaa
    41221 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
    41281 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
    41341 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
    41401 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
    41461 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
    41521 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
    41581 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
    41641 tttttgctga tgtgagtcca tcagcgccgc ggccagttct tctttcatct ccttatactg
    41701 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
    41761 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tagccaaaaa
    41821 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
    41881 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
    41941 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
    42001 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
    42061 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
    42121 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
    42181 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
    42241 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
    42301 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
    42361 aatgccggat tgctcggcga tgtttgccca atgagcgaac aaaatccgca ggtctggcag
    42421 gacgtcatgc agatcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
    42481 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
    42541 cgagccaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
    42601 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
    42661 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
    42721 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
    42781 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
    42841 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcccag gcccaggatg
    42901 aacgcggtat tatcatcgtc tttaccggca atggaaaagg caaaaccacc gcggcatttg
    42961 gtacggcaac acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag tttattaaag
    43021 gcacctggcc taatggcgaa cgcaatctgc tggagccaca tggcgttgag tttcaggtga
    43081 tggcaacggg ctttacctgg gatacacaaa accgcgagtc tgataccgcc gcctgccgcg
    43141 aagtctggca acatgcaaag cggatgcttg ctgattcctc actggatatg gttttgcttg
    43201 atgaactgac gtatatggtg gcgtatgact atttgccact ggaagaagtg gtgcaggcgt
    43261 taaatgaacg tccacatcaa cagacggtga ttatcacggg tcgaggctgt catcgggata
    43321 ttcttgaact ggcggatacg gtaagtgagc tgcgccccat cagacatgcg tttgatgccg
    43381 gtgttaaggc gcaaataggg attgattatt aatctgtaaa aatgccggat atgacgtccg
    43441 gcatactttg attaaccgtt attacgcccg ccagaacgac gactgccgct cacctgactg
    43501 tgacgtttca ccgcacgacg aatctgatta gccttcatgc gacgacggtc tttttctaca
    43561 gcgactttag agctggtttc tggcggaagc tccaccagtt cgcgcagata gttagtctgg
    43621 gcgaggtcca gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg gatatcaccg
    43681 taacgaacac ggatcaggcg gctcacctgc acaccaaccg cttcccacag acgacgaact
    43741 tcacggttac ggccttcggt cagagtcacg ttgtaccact ggttgatccc ttcgccgccg
    43801 ctgaacttga tggttttaaa agctgccgga ccatcttcca gttgcacgcc acgactcaaa
    43861 tcacgcagtt tcgcgtcgtc aacctgacca aatacacgca cggcgtattc acgttcaact
    43921 tcacggcttg gatgcattaa acggtttgcc agttcaccat cggtggtgaa cagcagcaga
    43981 ccacaggtgt taacgtccag acgacctacg gcaatccagc gtgcgccacg cagctttggc
    44041 agacggtcaa acaccgtcgg acgcccttcc ggatcgttac gggtacacaa ctcaccttcc
    44101 ggtttgtaat aggccagaac gcgacaaatt tgttcagcgg actcacgtac cgaaatcaga
    44161 tgaccatcga tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc gagtttagca
    44221 attttgccat caacactcac acgaccggct tcaataatag attcgatttc acggcgggag
    44281 ccgtggccag cgcgggccag cactttctgt aatttttcgc tcatagagct tccttttggt
    44341 gtcgccttca caggcgtcga acaggagaat caatggcgcc gtatagcgcc attttaaggc
    44401 cgcgtagtat acagagttac gcacttataa gaaaggcttc acatcaccta cgccttcacg
    44461 caccacgacc ggcgtatcat cggtaagatc aataaccgtt gttggtttct gcccgagata
    44521 gccgccgtga ataatcaaat ctacctgttt ttccagacgg tctttaattt cttcgggatc
    44581 ggattcggta aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg gttcgcccag
    44641 tgcttcaagc aacgcctgcg caatcgggtt agaaggcaca cgcataccga tggttttgcg
    44701 tttctcctgc aacagacggc gtggcacttc cttcgtcccc ttcaggatga aggtatagtt
    44761 gcccggcgtg ttgtttttca tcaaacgaaa cgcaacgtta tccacgaatg aataggtcga
    44821 gagttcagaa agatcgcgac acatcagggt aaagttgtga ccgtccggca gctggcgaat
    44881 acgacaaata cgctccatgg cgtttttatc ttcaatttta cagccgagcg catagccgga
    44941 atcagttgga tagacaatca ccccgccttt acgcacgatc tccaccgcct ggttgatcag
    45001 acgttgctgc gggttatcag gatgaatata aaaaaactgg ctcataattc cctctctgtg
    45061 gtgttctgcg gctgttccca tagctgccag acgccttcaa cgcctgcggg cagccagagt
    45121 ttacgaccca gttcgatcca cgggcatggc tgatgaaaat cagatccttg tgatgcccat
    45181 aaatgatgct gacgcgcaag tgtggccagc tgggtgcgtt cattgggcga ttgctggcac
    45241 tgcgcgactt ccatcgcgtc gccgtggtgt tcggcaaaat gcgcaaccaa tcttttcagc
    45301 catttagcac taagattgta ccgcccagga tgagctagca ccgccttacc gccagaatga
    45361 tgaatgacat caatagcttg ttctattgta caccactgtg gcggaacgta tccggttttc
    45421 ccgcgcgcca gatacttttt aaagacatct gccattgaac tggctttgcc gcactccaca
    45481 aggaaacgcg caaaatgacc gcgcgtcact gcgccgccct gcgccagtcg ttgtgcgcct
    45541 tccagcgccc caggtatttg cgctttttca agtctttcgg caatttgctg cgcccgctga
    45601 ttgcgccgtt ctgtctgctg cgcgaggaac tcacacatta gcggatgagt aatatcaata
    45661 ttcagcccga caatatgaat ttcatgattt tcccagaccg tggaaatttc cacgccggga
    45721 ataagattca gcgccagtcc cgaacgtgaa atttcctctc ttgctggcgc gattgcagct
    45781 gtggtgtcat ggtcggtgat cgccagggtg ccgacgcgca tctcgactgc acggtgcacc
    45841 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
    45901 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga
    45961 catcataacg ggtctggcaa atattctgaa atgagctgtt gacaattaat catcgaacca
    46021 gttaactagt acgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa ttttcgtact
    46081 gaaaggttgg tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt aaagcaatca
    46141 gatacccagc ccgcctaatg agcgggcttt tttttgaaca aaattagaga ataacaatgc
    46201 aaacacaaaa accgactctc gaactgctaa cctgcgaagg cgcttatcgc gacaacccga
    46261 ctgcgctttt tcaccagttg tgtgggaatc gtccggcaac gctgctgctg gaatccgcag
    46321 atatcgacag caaagatgat ttaaaaagcc tgctgctgat agacagtgcg ctgcgcatta
    46381 cagcattagg tgacaccgtc acaattcagg cactttccgg caatggcgaa gccctgctgg
    46441 cactactgga taacgccctg cctgcgggtg tggaaagtga acaatcacca aactgccgtg
    46501 tgctgcgctt cccccctgtc agtccactgc tggatgaaga cgcccgctta tgctcccttt
    46561 cggtttttga cgctttccgt ttattgcaga atctgttgaa tgtaccgaag gaagaacgag
    46621 aagcaatgtt cttcggcggc ctgttctctt atgaccttgt ggcgggattt gaagatttac
    46681 cgcaactgtc agcggaaaat aactgccctg atttctgttt ttatctcgct gaaacgctga
    46741 tggtgattga ccatcagaaa aaaagcaccc gtattcaggc cagcctgttt gctccgaatg
    46801 aagaagaaaa acaacgtctc actgctcgcc tgaacgaact acgtcagcaa ctgaccgaag
    46861 ccgcgccgcc gctgccagtg gtttccgtgc cgcatatgcg ttgtgaatgt aatcagagcg
    46921 atgaagagtt cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct ggagaaattt
    46981 tccaggtggt gccatctcgc cgtttctctc tgccctgccc gtcaccgctg gcggcctatt
    47041 acgtgctgaa aaagagtaat cccagcccgt acatgttttt tatgcaggat aatgatttca
    47101 ccctatttgg cgcgtcgccg gaaagctcgc tcaagtatga tgccaccagc cgccagattg
    47161 agatctaccc gattgccgga acacgcccac gcggtcgtcg cgccgatggt tcactggaca
    47221 gagatctcga cagccgtatt gaactggaaa tgcgtactga ccataaagag ctgtctgaac
    47281 atctgatgct ggttgatctc gcccgtaatg atctggcacg catttgcacc cctggcagcc
    47341 gctacatcgc cgatctcacc aaagttgacc gttattccta tgtgatgcac ctcgtctctc
    47401 gcgtagtcgg cgaactgcgt cacgatcttg acgccctgca cgcttatcgc gcctgtatga
    47461 atatggggac gttaagcggt gcgccgaaag tacgcgctat gcagttaatt gccgaggcgg
    47521 aaggtcgtcg ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg catggcgatc
    47581 tcgacacctg cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc accgtgcaag
    47641 cgggtgctgg tgtagtcctt gattctgttc cgcagtcgga agccgacgaa acccgtaaca
    47701 aagcccgcgc tgtactgcgc gctattgcca ccgcgcatca tgcacaggag actttctgat
    47761 ggctgacatt ctgctgctcg ataatatcga ctcttttacg tacaacctgg cagatcagtt
    47821 gcgcagcaat ggtcataacg tggtgattta ccgcaaccat attccggcgc agaccttaat
    47881 tgaacgcctg gcgacgatga gcaatccggt actgatgctt tctcctggcc ccggtgtgcc
    47941 gagcgaagcc ggttgtatgc cggaactcct cacccgcttg cgtggcaagc tgcccattat
    48001 tggcatttgc ctcggacatc aggcaattgt cgaagcttac gggggctatg tcggtcaggc
    48061 gggcgaaatt cttcacggta aagcgtcgag cattgaacat gacggtcagg cgatgtttgc
    48121 cggattaaca aacccgctgc cggtggcgcg ttatcactcg ctggttggca gtaacattcc
    48181 ggccggttta accatcaacg cccattttaa tggcatggtg atggcagtac gtcacgatgc
    48241 ggatcgcgtt tgtggattcc agttccatcc ggaatccatt ctcaccaccc agggcgctcg
    48301 cctgctggaa caaacgctgg cctgggcgca gcagaaacta gagccagcca acacgctgca
    48361 accgattctg gaaaaactgt atcaggcgca gacgcttagc caacaagaaa gccaccagct
    48421 gttttcagcg gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg cgctggtgag
    48481 catgaaaatt cgcggtgagc acccgaacga gatcgccggg gcagcaaccg cgctactgga
    48541 aaacgcagcg ccgttcccgc gcccggatta tctgtttgct gatatcgtcg gtactggcgg
    48601 tgacggcagc aacagtatca atatttctac cgccagtgcg tttgtcgccg cggcctgtgg
    48661 gctgaaagtg gcgaaacacg gtaaccgcag cgtctccagt aaatccggct cgtcggatct
    48721 gctggcggca ttcggtatta atcttgatat gaacgccaat aaatcgcgcc aggcgctgga
    48781 cgagttaggc gtatgtttcc tctttgcgcc gaaatatcac accggattcc gccacgcgat
    48841 gccggttcgc cagcaactga aaacccgcac cctgttcaat gtgctggggc cattgattaa
    48901 cccggcgcat ccgccgctgg cgttaattgg tgtttatagt ccggaactgg tgctgccgat
    48961 tgccgaaacc ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca gcggcgggat
    49021 ggatgaagtt tcattacacg cgccgacaat cgttgccgag ctgcatgacg gcaaaattaa
    49081 gagctatcaa ttgaccgctg aagattttgg cctgactccc taccaccagg agcaactggc
    49141 aggcggaaca cctgaagaaa accgtgacat tttaacacgc ttgttacaag gtaaaggcga
    49201 cgccgcccat gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc tgcatggcca
    49261 tgaagatctg caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg gttccgctta
    49321 cgacagagtt accgcgctgg cggcacgagg gtaaatgatg caaaccgttt tagcgaaaat
    49381 cgtcgcagac aaggcgattt gggtagaaac ccgcaaacag cagcaaccgc tggccagttt
    49441 tcagaatgag gttcagccga gcacacgaca tttttatgat gcgctacagg gtgcacgcac
    49501 ggcgtttatt ctggagtgta aaaaagcgtc gccgtcaaaa ggcgtgatcc gtgatgattt
    49561 cgatccggca cgcattgccg ccatttataa acattacgct tcggcaattt cggtgctgac
    49621 tgatgagaaa tattttcagg ggagctttga tttcctcctc atcgtcagcc aaatcgcccc
    49681 gcagccgatt ttatgtaaag acttcattat cgacccttac cagatctatc tggcgcgcta
    49741 ttaccaggcc gatgcctgct tattaatgct ttcagtactg gatgacgacc aatatcgcca
    49801 gcttgcagcc gtcgctcaca gtctggagat gggggtgctg accgaagtca gtaatgaaga
    49861 ggaactggag cgcgccattg cattaggggc aaaggtcgtt ggcatcaaca accgcgatct
    49921 gcgtgatttg tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac tggggcacaa
    49981 cgtaacggta atcagcgaat ccggcatcaa tacttacgct caggtgcgcg agttaagcca
    50041 cttcgctaac ggttttctga ttggttcggc gttgatggcc catgacgatt tgcacgccgc
    50101 cgtgcgccgg gtgttgctgg gtgagaataa agtatgtggc ctgacgcgtg ggcaagatgc
    50161 taaagcagct tatgacgcgg gcgcgattta cggtgggttg atttttgttg cgacatcacc
    50221 gcgttgcgtc aacgttgaac aggcgcagga agtgatggct gcggcaccgt tgcagtatgt
    50281 tggcgtgttc cgcaatcacg atattgccga tgtggtggac aaagctaagg tgttatcgct
    50341 ggcggcagtg caactgcatg gcaatgaaga tcagctgtat atcgatacgc tgcgtgaagc
    50401 tctgccagca catgttgcca tctggaaagc attaagcgtc ggtgaaaccc tgcccgcccg
    50461 cgagtttcag cacgttgata aatatgtttt agacaacggc cagggtggaa gcgggcaacg
    50521 ttttgactgg tcactattaa atggtcaatc gcttggcaac gttctgctgg cggggggctt
    50581 aggcgcagat aactgcgtgg aagcggcaca aaccggctgc gccggacttg attttaattc
    50641 tgctgtagag tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg ttttccagac
    50701 gctgcgcgca tattaaggaa aggaacaatg acaacattac ttaaccccta ttttggtgag
    50761 tttggcggca tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct ggaagaagct
    50821 tttgtcagcg cgcaaaaaga tcctgaattt caagcccagt tcaacgacct gctgaaaaac
    50881 tatgccgggc gtccaaccgc actgaccaaa tgccagaaca ttacagccgg gacgaacacc
    50941 acgctgtatc tcaagcgtga agatttgctg cacggcggcg cacataaaac caaccaggtg
    51001 ctggggcagg cgttgctggc gaagcggatg ggtaaaaccg aaatcatcgc cgaaaccggg
    51061 gccggtcagc atggcgtggc gtcggccctt gccagcgccc tgctcggcct gaaatgccgt
    51121 atttatatgg gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg tatgcgctta
    51181 atgggtgcgg aagtgatccc ggtacatagc ggttccgcca cgctgaaaga tgcctgtaat
    51241 gaggcgctac gcgactggtc cggcagttat gaaaccgcgc actatatgct gggcaccgcg
    51301 gctggcccgc atccttatcc gaccattgtc cgtgagtttc agcggatgat tggcgaagaa
    51361 accaaagcgc agattctgga aagagaaggt cgcctgccgg atgccgttat cgcctgtgtt
    51421 ggcggcggtt cgaatgccat cggcatgttt gctgatttca tcaatgaaac caacgtcggc
    51481 ctgattggtg tggagcctgg tggtcacggt atcgaaactg gcgagcacgg cgcaccgcta
    51541 aaacatggtc gcgtgggcat ttatttcggt atgaaagcgc cgatgatgca aaccgaagac
    51601 gggcagattg aagaatctta ctccatctcc gccggactgg atttcccgtc tgtcggtcca
    51661 caacacgcgt atcttaacag cactggacgc gctgattacg tgtctattac cgatgatgaa
    51721 gcccttgaag ccttcaaaac gctgtgcctg cacgaaggga tcatcccggc gctggaatcc
    51781 tcccacgccc tggcccatgc gctgaaaatg atgcgcgaaa acccggataa agagcagcta
    51841 ctggtggtta acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg
    51901 aaagcacgag gggaaatctg atggaacgct acgaatctct gtttgcccag ttgaaggagc
    51961 gcaaagaagg cgcattcgtt cctttcgtca cgctcggtga tccgggcatt gagcagtcat
    52021 tgaaaattat cgatacgcta attgaagccg gtgctgacgc gctggagtta ggtatcccct
    52081 tctccgaccc actggcggat ggcccgacca ttcaaaacgc cactctgcgc gcctttgcgg
    52141 caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga
    52201 ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt
    52261 tttatgccca gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg
    52321 aagagtccgc gcccttccgc caggccgcgt tgcgccataa tgtcgcacct atcttcatct
    52381 gcccgccaaa tgccgatgac gacctgctgc gccagatagc ctcttacggt cgtggttaca
    52441 catatttgct gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg ttacccctca
    52501 atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag gggtttggta
    52561 tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg
    52621 gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg
    52681 cactgaaagt ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc
    52741 agttccgctg gcggcatttt aactttcttt aatgaagccg gaaaaatcct aaattcattt
    52801 aatatttatc tttttaccgt ttcgcttacc ccggtcgaac gtcaacttac gtcatttttc
    52861 cgcccaacag taatataatc aaacaaatta atcccgcaac ataacaccag taaaatcaat
    52921 aattttctct aagtcacata ttcctcaggt aattgttaat atatccagaa tgttcctcaa
    52981 aatatatttc cctctatctt ctcgttgcgc ttaatttgac taatcctcat tagtgactaa
    53041 ttttaatgag tgtcgacaca caacactcat attaatgaaa caatgcaacg caacgggaga
    53101 aataacatgg ccgaacatcg tggtagttca ggaaatttcg ccgaagaccg tgagaaggca
    53161 tccgacgcag gccgtaaagg cggtcagcat agcggcggta attttaaaaa tgatccgcaa
    53221 cgcgcatctg aagcgggtaa aaaaggcggt caacaaagcg gtggtaataa atcaggcaaa
    53281 tcctgattca ccgttaatta ttaatatcag ctgataaaga taattgcatc tgcgggccgt
    53341 atggctcgca gtgatttcac tggagaaaaa tatgaatatg aagaccattg aagatgtatt
    53401 tattcaactg ctttcagata cctacagcgc agaaaaacaa ttaacccggg cactggcaaa
    53461 actcgcaaga gcaacatcaa atgaaaaatt aagtcaggct tttcatgcgc acctcgagga
    53521 aactcatgga cagattgaac gaattgatca agttgtggaa tcagaatcaa atctgaaaat
    53581 taagcgcatg aaatgtgtgg caatggaagg tcttattgaa gaagctaatg aggtcatcga
    53641 aagtaccgag aaaaacgaag tgcgtgatgc cgcactgatt gccgcagcac agaaagtcga
    53701 gcattatgag attgccagtt acgggacatt agcgacgctg gctgaacaat taggttaccg
    53761 taaagcagcg aagcttctga aagaaaccct ggaagaagaa aaggccaccg acatcaaact
    53821 gactgatctg gcccttaata acgtaaataa gaaagccgaa aataaagcct gaaatatgaa
    53881 ttttaacttt tagtcatttt ataaagagga cattttcatg aatcgtattg aacattatca
    53941 tgactggtta cgtgacgccc acgcaatgga aaagcaagcc gaatctatgc ttgaatccat
    54001 ggccagccgt atagataatt atcctgaact acgcgctcgt attgaacaac atcttagtga
    54061 aaccaaaaac cagattgttc aactggaaac tattcttgat cgtaatgaca tttcacgttc
    54121 agtcattaaa gattccatga gtaaaatggc tgcgcttggg cagtcaatcg gtggtatatt
    54181 cccttctgat gaaatagtta aaggctctat tggcggatat gtcttcgagc aatttgaaat
    54241 cacctgttac acctcactat tagcagcagc aaaaaatgcc ggtgatacag tctcaattcc
    54301 gatcatcgaa gcgattttaa atgaggaaaa gcaaatggcc gactggctga ttcagcatat
    54361 tccgcaaaca actgagaaat ttttaattcg ttctgaaact gatggcgtag aagcgaagaa
    54421 ataataccct ttataccatg tccttattga ccccgtatat tacggggtcg tttttgtgcg
    54481 gaattaaaaa cgatatcctg ctgagaacat aaacacccac ggatcgaggc gtacgctatc
    54541 gtgttgctgt gcaccgccta gcttataatt ggcggtggta tcgatatcca tgtaccacac
    54601 tgacatgtta accaaccagt cacggttaat cagataatca acccccacct gcccggcagc
    54661 tccccaggaa tctttcagac tgagatcgga aagccccgcc tctttgccat gatcgttaaa
    54721 tccattatca aagaaggtgg tgtagttaat accggcccca acgtaaggac ggaatttgct
    54781 gctggcatca ccaaaatacc actgcgccat cagtgttggc ggcagatggt gaacggttgc
    54841 aatatcgccg gtcgcccggg tgccgatttt atggcggaac ggcgtcgctg ccagtaattc
    54901 cacaccaatg ttgtcggtcg ccatataagt aaacgtcaag cccagttgag tgttattggt
    54961 cacgctgaat ccacccagac ttcctaacgt accaccagca ccttctgttg gacgtacggt
    55021 tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc gcaaaggcac tgccagaaag
    55081 aagagttgtt actgccaaag ccgccactgt taactttttc atatccgctc cgtcgttatg
    55141 gttataaaaa cgtgacgaat atacctacat tggagtaaca aatgatccta catagatcac
    55201 attacatatg ataattcacc atttattgat ctggattaat ttagaagttg cattgagaat
    55261 cctggttaat gtgatttaaa tcaattttta aatattttct ctacgcaaat attaactcga
    55321 tcgccacttg ccgcgctttg cccacgcctg aaggccagca attacctccg ctttacaaag
    55381 atatgctttt ccatacaaga cctgatgctg ccacccgagg cttaaccagg gtacaattgc
    55441 ccgctaatta acacctgcat aaaactcaag gagagtgcat gtctatcacg gcgcagtccg
    55501 tataccgtga caccggaaat ttcttccgta atcaatttat gaccattctg ttggtatcgt
    55561 tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt ctcacccagt gatgcacagc
    55621 ttgcgcagct caatgacggc gtgcccgtta gcggcagtag tgggttgttc gacctggttc
    55681 agaatatgtc accggaacag caacaaattt tgctgcaggc ttcagcggcg tccacttttt
    55741 cagaattaat cggtaacgcc attctcgccg gaggcgtaat attaattatc cagctggtgt
    55801 ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc cagtgcgccg atattgccca
    55861 agttatttat tctgattttt ctgactaccc ttttagtaca gattggcatc atgctggtgg
    55921 tcgttccggg aattatcatg gccattttac tggccctggc accggtgatg ctggtacagg
    55981 acaaaatggg catttttgcc tcgatgcgta gcagtatgcg gctgacttgg gcgaatatgc
    56041 gtctggtggc acccgcagta ctgagctggt tgctggcaaa aacactgttg ctgctttttg
    56101 cctcttcttt tgccgcatta accccggaaa ttggtgccgt actggcgaac accttgagca
    56161 acctgatttc tgccatattg ctcatctatc tgttccgcct gtatatgttg attcgccaat
    56221 aaccttaagc atttgattac ggaatcgtaa aatgaagcag tttcttgatt ttttaccgct
    56281 ggttgtcttt ttcgcgtttt acaagattta tgacatctat gcggctactg cagcgctgat
    56341 cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc tttcgtaagg ttgagaagat
    56401 ggccctgatc acttttgttc tggtggtcgt cttcggtggc ttgacgctgt tcttccacaa
    56461 tgatgagttt attaaatgga aggttacagt catttatgct ctgtttgcgg gagccctgtt
    56521 agtcagccaa tgggtgatga aaaagccgct aattcagcgg atgctgagta aagaactcac
    56581 gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg gctgttttct ttatcctttg
    56641 cggtctggca aacatctaca tcgcattctg gctgccgcaa aatatttggg tcaactttaa
    56701 agtctttggc ctgaccgccc ttaccttaat cttcacattg ttaagcggta tctatatcta
    56761 ccgccacatg ccgcaggaag ataaatccta accagactgc cagtccttcg gggctggcgc
    56821 tgtatctcct ctgttctcct caggaaaatc atagtagcat cgcgcctgtg attttccttt
    56881 taagtcggtt ttaccatgtc tacaacacat aacgtccctc agggcgatct tgttttacgt
    56941 actttagcca tgcccgccga taccaatgcc aatggtgaca tctttggtgg ttggttaatg
    57001 tcacaaatgg atattggcgg cgctattctg gcaaaagaaa ttgcccacgg tcgcgtagtg
    57061 actgtgcggg ttgaaggaat gactttctta cggccggttg cggtcggcga tgtggtgtgc
    57121 tgctatgcac gctgtgtcca gaaagggacg acatcggtca gcattaatat tgaagtgtgg
    57181 gtgaaaaaag tagcgtctga accaatcggg caacgctata aagcgacaga agcattattt
    57241 aagtatgtag cggttgatcc tgaaggaaaa cctcgcgcct tacctgttga gtaatagtca
    57301 aaagcctccg gtcggaggct tttgactttc tgcttactga atttcggtgg tgccgttaat
    57361 tttaaacagg atattcacca caatcccact gcctggctta cccggctcat aacgccatct
    57421 gcgcatcgca tttttcacct cacgctcaaa catgttcgca ggcttggctg agaggatttg
    57481 tacgttatcc acgcgaccat ccggcgtgac gtcaaattta actttaacct gcccttcaat
    57541 gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta cggcttaatg cgcgtggtcc
    57601 tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt gccgtacttg atgtggggcg
    57661 cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac tctacgggtt tgacatcgcg
    57721 ttttggctgc tcctgtacct ttttcaccgg cttcggtttt ggcttaggtt tcggcttcgg
    57781 cttttcaatg accaccggtg cttcttttgg cggttcgggg atcggctcag gttccggttc
    57841 tggctctacc accggctccg gtggcggctg aacggcttgt ggcggttcga gatcagcagg
    57901 cgcaaccatc gtgacagaaa ttggttgcgc aggcgcaggt agttcaataa cctgatgtac
    57961 cgaggtatag agcagccccg ccacaacagc accatgaatg cagaccgaaa gtaacgtcgg
    58021 ccaggggaag cggcgaggta aatcaagggt tattgaagtc ataatcattt cagtagaaaa
    58081 actaggcctc gattttaaat gcaaatagca atcatattca ataaggcaag acg
//
LOCUS       DAJTYM010000013        57328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTYM010000013 DAJTYM010000000
VERSION     DAJTYM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..302
                     /locus_tag="OO575_000813"
     CDS             <1..302
                     /locus_tag="OO575_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Stimulates the peptidyltransferase activity of the
                     70S ribosome and enhances dipeptide synthesis with
                     N-formylmethionyl-tRNA and puromycin in vitro, suggesting
                     its involvement in the formation of the first peptide bond
                     of a protein; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6663883.1"
                     /translation="PYTFTKDQIEEELLFMPEGGMPDMQVLTWDGQLLALELPQTVDL
                     EIVETAPGIKGASASARNKPATLSTGLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            525..1991
                     /locus_tag="OO575_000814"
     CDS             525..1991
                     /locus_tag="OO575_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6663884.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2109..3095
                     /gene="yeiR"
                     /locus_tag="OO575_000815"
     CDS             2109..3095
                     /gene="yeiR"
                     /locus_tag="OO575_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6663885.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3134..3847
                     /gene="lpxT"
                     /locus_tag="OO575_000816"
     CDS             3134..3847
                     /gene="lpxT"
                     /locus_tag="OO575_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6663886.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4259..4825
                     /gene="mepS"
                     /locus_tag="OO575_000817"
     CDS             4259..4825
                     /gene="mepS"
                     /locus_tag="OO575_000817"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6663887.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5006..6562
                     /locus_tag="OO575_000818"
     CDS             5006..6562
                     /locus_tag="OO575_000818"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6663888.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            6644..8458
                     /locus_tag="OO575_000819"
     CDS             6644..8458
                     /locus_tag="OO575_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6663889.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            8459..9553
                     /locus_tag="OO575_000820"
     CDS             8459..9553
                     /locus_tag="OO575_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6663890.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            9553..10578
                     /locus_tag="OO575_000821"
     CDS             9553..10578
                     /locus_tag="OO575_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6663891.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            10580..12169
                     /gene="yejF"
                     /locus_tag="OO575_000822"
     CDS             10580..12169
                     /gene="yejF"
                     /locus_tag="OO575_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6663892.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12173..12517)
                     /locus_tag="OO575_000823"
     CDS             complement(12173..12517)
                     /locus_tag="OO575_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663893.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(12850..14040)
                     /gene="bcr"
                     /locus_tag="OO575_000824"
     CDS             complement(12850..14040)
                     /gene="bcr"
                     /locus_tag="OO575_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6663894.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14068..14763)
                     /gene="rsuA"
                     /locus_tag="OO575_000825"
     CDS             complement(14068..14763)
                     /gene="rsuA"
                     /locus_tag="OO575_000825"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6663895.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            14913..16673
                     /locus_tag="OO575_000826"
     CDS             14913..16673
                     /locus_tag="OO575_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6663896.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            16798..17082
                     /gene="rplY"
                     /locus_tag="OO575_000827"
     CDS             16798..17082
                     /gene="rplY"
                     /locus_tag="OO575_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6663897.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17221..18228)
                     /gene="yejK"
                     /locus_tag="OO575_000828"
     CDS             complement(17221..18228)
                     /gene="yejK"
                     /locus_tag="OO575_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6663898.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18410..18637
                     /locus_tag="OO575_000829"
     CDS             18410..18637
                     /locus_tag="OO575_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6663899.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            18657..20417
                     /gene="pbgA"
                     /locus_tag="OO575_000830"
     CDS             18657..20417
                     /gene="pbgA"
                     /locus_tag="OO575_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6663900.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            20492..20568
                     /locus_tag="OO575_000831"
     tRNA            20492..20568
                     /locus_tag="OO575_000831"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20526..20528,aa:Pro,seq:ggg)
     gene            complement(20671..23262)
                     /gene="yejO"
                     /locus_tag="OO575_000832"
     CDS             complement(20671..23262)
                     /gene="yejO"
                     /locus_tag="OO575_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6663901.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            23582..24229
                     /gene="narP"
                     /locus_tag="OO575_000833"
     CDS             23582..24229
                     /gene="narP"
                     /locus_tag="OO575_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6663902.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(24440..25492)
                     /gene="ccmH"
                     /locus_tag="OO575_000834"
     CDS             complement(24440..25492)
                     /gene="ccmH"
                     /locus_tag="OO575_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6663903.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(25489..26046)
                     /gene="dsbE"
                     /locus_tag="OO575_000835"
     CDS             complement(25489..26046)
                     /gene="dsbE"
                     /locus_tag="OO575_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6663904.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26043..27986)
                     /gene="ccmF"
                     /locus_tag="OO575_000836"
     CDS             complement(26043..27986)
                     /gene="ccmF"
                     /locus_tag="OO575_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6663905.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(27983..28462)
                     /gene="ccmE"
                     /locus_tag="OO575_000837"
     CDS             complement(27983..28462)
                     /gene="ccmE"
                     /locus_tag="OO575_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6663906.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(28459..28668)
                     /gene="ccmD"
                     /locus_tag="OO575_000838"
     CDS             complement(28459..28668)
                     /gene="ccmD"
                     /locus_tag="OO575_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6663907.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(28665..29402)
                     /gene="ccmC"
                     /locus_tag="OO575_000839"
     CDS             complement(28665..29402)
                     /gene="ccmC"
                     /locus_tag="OO575_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6663908.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(29444..30106)
                     /gene="ccmB"
                     /locus_tag="OO575_000840"
     CDS             complement(29444..30106)
                     /gene="ccmB"
                     /locus_tag="OO575_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6663909.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30103..30720)
                     /gene="ccmA"
                     /locus_tag="OO575_000841"
     CDS             complement(30103..30720)
                     /gene="ccmA"
                     /locus_tag="OO575_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6663910.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(30739..31341)
                     /gene="napC"
                     /locus_tag="OO575_000842"
     CDS             complement(30739..31341)
                     /gene="napC"
                     /locus_tag="OO575_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6663911.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31351..31800)
                     /gene="napB"
                     /locus_tag="OO575_000843"
     CDS             complement(31351..31800)
                     /gene="napB"
                     /locus_tag="OO575_000843"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6663912.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(31797..32660)
                     /gene="napH"
                     /locus_tag="OO575_000844"
     CDS             complement(31797..32660)
                     /gene="napH"
                     /locus_tag="OO575_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6663913.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(32647..33342)
                     /gene="napG"
                     /locus_tag="OO575_000845"
     CDS             complement(32647..33342)
                     /gene="napG"
                     /locus_tag="OO575_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6663914.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33349..35835)
                     /gene="napA"
                     /locus_tag="OO575_000846"
     CDS             complement(33349..35835)
                     /gene="napA"
                     /locus_tag="OO575_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6663915.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(35832..36095)
                     /gene="napD"
                     /locus_tag="OO575_000847"
     CDS             complement(35832..36095)
                     /gene="napD"
                     /locus_tag="OO575_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6663916.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36085..36579)
                     /gene="napF"
                     /locus_tag="OO575_000848"
     CDS             complement(36085..36579)
                     /gene="napF"
                     /locus_tag="OO575_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6663917.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            36688..36852
                     /locus_tag="OO575_000849"
     CDS             36688..36852
                     /locus_tag="OO575_000849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663918.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            36987..37475
                     /gene="eco"
                     /locus_tag="OO575_000850"
     CDS             36987..37475
                     /gene="eco"
                     /locus_tag="OO575_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6663919.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(37597..38628)
                     /locus_tag="OO575_000851"
     CDS             complement(37597..38628)
                     /locus_tag="OO575_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6663920.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(38862..39221)
                     /locus_tag="OO575_000852"
                     /pseudo
     CDS             complement(38862..39221)
                     /locus_tag="OO575_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(39439..41082)
                     /gene="yojI"
                     /locus_tag="OO575_000853"
     CDS             complement(39439..41082)
                     /gene="yojI"
                     /locus_tag="OO575_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6663921.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41158..41808)
                     /gene="alkB"
                     /locus_tag="OO575_000854"
     CDS             complement(41158..41808)
                     /gene="alkB"
                     /locus_tag="OO575_000854"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6663922.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(41808..42872)
                     /gene="ada"
                     /locus_tag="OO575_000855"
     CDS             complement(41808..42872)
                     /gene="ada"
                     /locus_tag="OO575_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6663923.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(42946..44001)
                     /gene="apbE"
                     /locus_tag="OO575_000856"
     CDS             complement(42946..44001)
                     /gene="apbE"
                     /locus_tag="OO575_000856"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6663924.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44113..45234)
                     /gene="ompC"
                     /locus_tag="OO575_000857"
     CDS             complement(44113..45234)
                     /gene="ompC"
                     /locus_tag="OO575_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6663925.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            45972..48644
                     /gene="rcsD"
                     /locus_tag="OO575_000858"
     CDS             45972..48644
                     /gene="rcsD"
                     /locus_tag="OO575_000858"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6663926.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            48661..49311
                     /gene="rcsB"
                     /locus_tag="OO575_000859"
     CDS             48661..49311
                     /gene="rcsB"
                     /locus_tag="OO575_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6663927.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(49511..52360)
                     /gene="rcsC"
                     /locus_tag="OO575_000860"
     CDS             complement(49511..52360)
                     /gene="rcsC"
                     /locus_tag="OO575_000860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6663928.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(52635..53411)
                     /locus_tag="OO575_000861"
     CDS             complement(52635..53411)
                     /locus_tag="OO575_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6663929.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53416..55065)
                     /locus_tag="OO575_000862"
     CDS             complement(53416..55065)
                     /locus_tag="OO575_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6663930.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEEELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55066..55625)
                     /locus_tag="OO575_000863"
                     /pseudo
     CDS             complement(55066..55625)
                     /locus_tag="OO575_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(55784..>57328)
                     /locus_tag="OO575_000864"
     CDS             complement(55784..>57328)
                     /locus_tag="OO575_000864"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6663931.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14019 a        15490 c        14075 g        13744 t
ORIGIN      
        1 ccccgtatac cttcaccaaa gatcagattg aagaagagtt gctgtttatg ccagagggcg
       61 gcatgccgga catgcaggtg ctgacctggg acggtcaact gctggcgctt gagctgccgc
      121 agaccgttga tctggaaatc gttgaaaccg caccaggcat caaaggggca tctgccagcg
      181 cccgtaacaa accggcgaca ttgagcactg gtctggtgat tcaggtacca gaatacttaa
      241 gcccgggcga aaaaattcgt atccatatcg aagaacgccg ttatatgggg cgtgctgact
      301 aacttcagcc gcatgcagaa aagggatagc tcaggctgtc ccttttttaa tttattatac
      361 caatcttcta ttttgcgctt cctgaacacc ttacgccctc aattttcact cgttgattga
      421 tcgccctcac acttcatcgc attaacaatc cagaccaatt tcaattgctg tcatataacc
      481 ttacactgtc attgttaatt tatcgttact aagacgtgac tcctatgaag acaattgcct
      541 ccgttacgct cccgcatcat gtacacgctc cacgctacga tcgccagcag ttgcaatcac
      601 gtatcgttca ttttggcttt ggcgcctttc accgcgctca tcaggcgtta ctgaccgatc
      661 gtgtgctgaa tgcccagggc ggcgactggg ggatctgtga aatcagcttg ttcagcggtg
      721 atcaactgat gagccagctc cgcgcacaga accatttata taccgtgctg gagaaaggtg
      781 cggacggcaa tcaggtgata attgtcggtg ccgttcacga atgccttaat gcaaaactgg
      841 attccttagc ggcaattatt gagaaatttt gcgagccaca ggtggcaatt gtttccctga
      901 cgattaccga aaaaggctat tgtattgacc cggccaccgg tgcactcgac accagtaatc
      961 cgcggattat tcacgatcta caaacccctg aagaacctca ctccgcaccg ggtattctcg
     1021 tcgaagcact gaaacgccgc cgtgagcgca gccttacacc gtttaccgtg ctctcctgcg
     1081 acaatattcc cgacaatggc catgtggtga aaaacgcggt gctgagaatg gcagaaaaac
     1141 gttcgccaga actcgccggg tggataaaag aacacgtcag ttttccggga accatggtcg
     1201 accgcattgt tccggctgca accgacgaat cactggcaga aatcagccag catctggggg
     1261 taaatgatcc ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtt gaagataact
     1321 tcatcgctgg gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat gatgtcctgc
     1381 catgggaaga gatgaaactg cgtatgctta atggcagcca ctcttttctc gcttatctgg
     1441 gttacctctc aggattcgcc tatatcagtg attgcatgca ggatcgcgta tttcgctatg
     1501 ctgccagaac attaatgctg gatgagcaag cgccgacact gcaaattaaa gatgtcgatt
     1561 taacacaata tgcggataag ttaattgcac gttttgctaa tccgtcgctg aaacataaga
     1621 cctggcaaat cgcgatggat ggcagccaga aattaccgca acgtatgctg gcaggtattc
     1681 gcatacatct ggggcgtgaa acggactggt cgttgctggc attaggcgtt gctggctgga
     1741 tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc gatccgctta
     1801 gcgataaaat tcgcgaactt gttgcgggca gcagcagtga acaacgcgta accgccctgc
     1861 tttccctgcg tgaagttttc ggtgatgatc tgccagataa cccgcatttt gtgcaggcca
     1921 tcgaacaagc ctggcaacaa atcgtacaat tcggcgcaca tcaggcgcta ttaaacaccc
     1981 tcaaaattta acgatttctg cggttaaagc ggatgaagct caccttcgtc cgctctcccc
     2041 ttctcttttc tgcctttttt agccaggatt aacgctcagt taacttacca gagtattgcg
     2101 gagccgttgt gaccaggacc aacctcatca ccggttttct cggcagcggg aaaaccacgt
     2161 cgattcttca tctgttagcc cataaagatc ccaacgaaaa atgggcggta ctggttaatg
     2221 aatttgggga agtcggaatt gatggtgctt tgctcgccga tagcggcgca ttgctgaaag
     2281 agatccccgg cggctgcatg tgctgcgtta atggtttacc catgcaggta gggttgaata
     2341 ccttactgcg tcagggaaaa ccagaccgct tgttgataga gccgaccggg ctgggccatc
     2401 cgaaacagat cctcgatctg ttaaccgcac cagtctatga accgtggata gatctgcgcg
     2461 ccaccttgtg cattctcgat ccacgcctgc tgctggacga aaaaagcgcc agcaatgaaa
     2521 acttccgtga ccagctggct gccgcagaca tcattgtcgc caataaatcc gaccgtgcga
     2581 cgcccgaaag tgagcaagcg ctacagcgtt ggtggcagca aaatggtggc gatcgacaat
     2641 taattcacag tgagcatggg aaagttgacg gtcatcttct ggatttgccg cgtcgcaatt
     2701 tagccgagtt gcccgccagc gccgcgcatt ctcatcagca tgtcgtgaaa aaagggttag
     2761 cagcgttaag cctgccagag catcaacgct ggcgtcgcag tctgaacagc gggcaaggat
     2821 atcaggcctg cggctggata ttcgacgctg atacggtatt cgacaccatt ggcattctgg
     2881 aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt gctgcgtatt cccgaagggc
     2941 tggtacgaat caaccgtcag ggcgatgacc tgcacattga aacgcaaaac gttgcgccac
     3001 cggacagccg tattgagctg atttccagca gcgaagctga ctggaatgcc ttgcagagcg
     3061 cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt tcagtaagat
     3121 aattagagaa aatatgatta aaaatttgcc gcaaatagtg ttgttgaata ttgtcggccc
     3181 cgcgctgttt ctttcctggt atattcccgt taatcatgga ttctggttgc cgattgatgc
     3241 ggatattttt tatttcttta atcagaaact ggtcgaaagt aaggcctttt tatggctggt
     3301 tgcattgact aacaatcgcg ccttcgacgg ttgttcactg ctggcgatgg gtatgttgat
     3361 gctgagtttc tggctgaaag aaaacgcccc tggcagacga cgtatcgtga ttatgggtct
     3421 ggtcatgcta ttaactgcag tggtattaaa ccagctgggt caggcattaa ttcctgtaaa
     3481 acgggccagc ccaacattga cttttaccga tattaaccgc gtcagcgaac tgctctctgt
     3541 tcccacgaaa gatgcctcac gagatagctt tcccggcgat cacggcatga tgctgcttat
     3601 tttttcggca ttcatgtggc gttatttcgg caaagttgca ggccttatcg cccttattat
     3661 ttttatggtt tttgcatttc ccagagtaat gattggcgca cactggttta ctgacatcat
     3721 tgtcggttcg atgaccgtga tattgatcgg tttgccctgg gtgttgctga cgccattaag
     3781 tgatcgatta atcacctttt ttgacaaatc actaccagga aaaaacaaac atttccaaaa
     3841 caaataactc acagtaatta acatcatcag ggttattttt atagtgagga taatcctgat
     3901 gatgcgcacc gtgctttcat ctatcgaacg caaaaatcat tctctaagta aatgaatgga
     3961 ttgcatgcgt ttcactcaat tgtactttaa ttgaccaacc ccgcttatta actttctgta
     4021 tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc aatcgggtaa
     4081 tctcgaactc gttttgcctc ggcggtagat tatcctcaca gcatataatt ttgtgcgtta
     4141 gtccacagat ttggccttaa ggaattgttt caacatgccc aggtaattag tctcgtgtcg
     4201 cttggcattt ttttataacg atatttgtcg ttaaggactt caagggaaaa caaacaacat
     4261 ggtcaaatct caaccgattt tgagatatat cttgcgcggg attcccgcga ttgcagtagc
     4321 ggttctgctt tctgcatgta gtgcaaataa caccgcaaag aatatgcatc ctgagacacg
     4381 tgcagtgggt agtgaaacat catcactgca agcttctcag gatgaatttg aaaacctggt
     4441 tcgtaatgtc gacgtaaaat cgcgaattat ggatcagtat gctgactgga aaggcgtacg
     4501 ttatcgtctg ggcggcagca ctaaaaaagg tatcgattgt tctggtttcg tacagcgtac
     4561 attccgtgag caatttggct tagaacttcc gcgttcgact tacgaacagc aggaaatggg
     4621 taaatctgtt tcccgcagta atttgcgtac gggtgattta gttctgttcc gtgccggttc
     4681 aacgggacgc catgtcggta tttatatcgg caacaatcag tttgtccatg cttccaccag
     4741 cagtggtgtt attatttcca gcatgaatga accgtactgg aagaagcgtt acaacgaagc
     4801 acgccgggtt ctcagccgca gctaataaac cgtttggatg caatcccttg gctatcctga
     4861 cgagttaact gaaagcactg cttaggcagt gcttttttgt tttcattcat cagagaaaat
     4921 gatgtttccg cgtcttgatc caggctatag tccggtcatt gttatctttt aaatgttgtc
     4981 gtaatttcag gaaattaacg gaatcatgtt catacgcgct cccaattctg gacgtaagct
     5041 cctgcttacc tgcattgtag caggcgtgat gattgcgata ctggttagct gccttcagtt
     5101 tttagtggcc tggcataagc acgaagtcaa atacgacaca ctgattatcg acgtacaaaa
     5161 gtatctcgat acctattttg ccgacctgaa atccactact gaccggcttc agccgctgac
     5221 cttagatacc tgccagcagg ctaaccccga actgaccgct cgcgcggcgt ttagcatgaa
     5281 tgtccgtacg tttgtgctgg tgaaagataa aaaaacattc tgttcatctg cgactggtga
     5341 gatggacatt ccactcaatg aattgattcc ggcgctcgac attaataaaa acgtcgatat
     5401 ggcgatctta cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca tctggtatcg
     5461 caaccctttg ctgaaaaata gcggtgtgtt tgccgctctg aatctcaacc tgacgccttc
     5521 tctcttttat agttcacggc aggaagatta cgatggcgtc gccctcatta ttggcaatac
     5581 tgcgctatct accttttctt cacgtttgat gaacgttaat gaattaaccg acatgccggt
     5641 ccatgaaact aaaattgcgg gcattcctct gaccgttcgg ctttatgcgg atgactggac
     5701 atggaacgat gtgtggtacg catttttact gggcggcatg agtggaactg tcgttggcct
     5761 gctttgctat tacctgatga gcgtacgtat gcgccccgac agagaaatca tgaccgccat
     5821 caagcgcgaa caattttacg tggcgtatca accggtggtg gatacacaag ctttgcgggt
     5881 aacgggcctg gaagtactgc tacgctggcg gcatcctgta gcgggagaaa ttcccccgga
     5941 tgccttcatt aactttgccg aatcgcaaaa gatgattgtg ccgctgactc agcacctgtt
     6001 tgagttaatt gcccgcgatg ccgcagaatt agaaaaagta ctgccggtag gcgtcaaatt
     6061 tggcattaac attgcgccgg accatctgca cagcgaaagc tttaaagcag atatccagaa
     6121 actgctcact tccctgcccg cacaccattt ccagattgtg ctggaaatta ccgagcgcga
     6181 tatgctgaaa gagcaagaag ccacacaact cttcgcctgg ctgcactcgg tcggcgtaga
     6241 aattgctatt gatgacttcg gcaccgggca cagcgcgctt atctatcttg agcgttttac
     6301 gctcgattat ctgaaaattg accgtggatt tatcaacgcc atcggtacgg aaacgatcac
     6361 ttcacccgta cttgacgcgg tgctgacgct ggcgaaacgt ctcaatatgc tgacagttac
     6421 tgaaggggtc gaaacgccgg aacaagcgcg atggctaagc gaacgcggcg ttaatttcat
     6481 gcaaggctac tggattagtc acccgttacc gctggacgat tttgttcgct ggctgaagaa
     6541 accgtatacg ccgcagtggt aaggtgtgct tacgtccctt attattcata gtgaaagcat
     6601 gccggattgc ggctaatgat gagtaaaagg aaatccgttg cagatgattg tgcgcatact
     6661 gctgctgttt atcgctctgt tcaccttcgg tgcgcaggcg caggctatca aggaaagcta
     6721 tacctttgcc gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt ttgattatgt
     6781 gaaccccgcc gcgccaaaag gtgggcagat aacgttgtca gccctcggta ccttcgataa
     6841 tttcaaccgc tatgcactgc gcggcaaccc gggcgcacgc accgagcagc tgtacgacac
     6901 gttatttacg acttccgatg acgaaccagg cagttattac ccgctgattg ctgaaagtgc
     6961 acgctatgct gacgattatt cctgggtgga ggtcgctatt aatccgcgcg cccgttttca
     7021 tgatggttct cccattactg cccgcgatgt agagtttact tttcaaaaat ttctgaccga
     7081 aggcgtgccg caatttcgtc tggtctacaa aggcaccacc gtcaaagcca ttgcgccgtt
     7141 aaccgtgcgc attgagttag ctaaacccgg caaagaagat atgttgagtc tgttttcgct
     7201 gccggtattt ccagaaaagt actggaagga tcacaaactt agcgacccgc tcgccacgcc
     7261 tccgcttgcc agtggtccgt accacgttac gtcctggaaa atggggcaaa atattgtcta
     7321 ttcccgcgtg aaagattact gggcagcaaa cttaccggta aaccgtggac gctggaattt
     7381 cgacaccatt cgctacgatt attacctcga tgataatgtc gcctttgaag cgtttaaagc
     7441 aggtgccttt gatttgcgta tggaaaacga cgctaaaaac tgggccacgc gttataccgg
     7501 taaaaatttc gataaaaaat acatcatcaa agatgagcaa aagaacgaat cagcccagga
     7561 tacgcgctgg ctggcgttta atatccaacg tgcggtattc agcgatcgcc gggtgcggga
     7621 agcaatcact ctcgcctttg actttgaatg gatgaacaag gcgttgtttt acaacgcctg
     7681 gagtcgcacg aacagttatt ttcaaaatac cgaatacgcg gccagaaatt accccgacgc
     7741 cgcggagctg gtgcttctgg caccaatgaa aaaagatcta ccgccagaag tcttcacaca
     7801 aatctaccag ccgccggtat ctaaaggcga tggctacgat cgtgacaacc tgttaaaagc
     7861 cgacaaactt ctcaacgaag cgggctgggt gctgaagggt cagcaacgcg ttaatgccac
     7921 aacgggtcag ccgctcagct ttgaattatt gcttcccgca agcagcaata gtcagtgggt
     7981 attgccgttc cagcacagcc tgcaacggct gggtatcaat atggacattc gcaaggtgga
     8041 taactcgcaa atcactaacc gcatgcgcaa tcgcgactat gacatgatgc cgcgactatg
     8101 gcgggcgatg ccgtggccca gttccgattt acagatttcc tggtcatcgg aatatatcaa
     8161 ttccacttat aatgcccccg gcgtgcaaag cccggttatc gactcactga tcaaccaaat
     8221 tattgccgcg cagggaaata aagaaaaatt actgccgttg gggcgagcac tggatcgcgt
     8281 attaacgtgg aattattaca tgctgccaat gtggtacatg gcggaagacc gtctcgcctg
     8341 gtgggataaa ttctcccaac ccgctgtacg ccctgtttac agcctgggta tcgatacctg
     8401 gtggtatgac gttaataaag cggccaaact gccgtcagcc agacaacagg gagagtagat
     8461 gggcgcttac ctgattcgcc gtctgttgct ggtgatccca acgttatggg ctattatcac
     8521 tatcaacttt ttcatcgtgc aaattgcgcc tggcggtccg gtcgatcagg ccatcgccgc
     8581 cattgagttt ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg ttcgtgccag
     8641 ccatgcgcaa acgggtgtcg gcaatatcag cgacagtaat taccgtggcg gacgcggatt
     8701 agatccagaa gtgatcgctg agatcactca tcgctacggt ttcgataagc cgatccacga
     8761 acgttacttc aaaatgctct gggactacat ccgctttgat tttggtgata gcctgtttcg
     8821 cagcgcctcg gtgctgacgc tgattaaaga cagtctgccg gtttccatca ccctcggatt
     8881 gtggagcacg ctgattatct atctggtgtc gattccgtta ggcattcgca aagctgttta
     8941 taatgggagt cgctttgacg tctggagtag cgcatttatc atcatcggct acgccattcc
     9001 ggcctttttg tttgccatcc tgctgattgt cttcttcgcg ggcggcagct atttcgacct
     9061 gttccctcta cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt atcagaaaat
     9121 caccgattat ctgtggcata tcacgctacc ggtgctggcg acagtgattg gtggctttgc
     9181 ggcgctgacc atgctgacca aaaactcatt ccttgatgaa gtgcgcaagc aatacgtggt
     9241 gaccgcgcgt gcgaaagggg taagtgagaa aaacattctc tggaaacata tgttccgcaa
     9301 cgccatgctg ctggtgattg ccggttttcc ggcgacgttt atcagcatgt tttttaccgg
     9361 ctcgctgctg attgaggtga tgttttcact caatggtctt ggcttactgg gctacgaagc
     9421 gaccgtctcg cgcgattatc ctgtaatgtt tggtaccttg tatattttca ccctgattgg
     9481 cctgctgctg aatattgtca gtgatatcag ctatacgctg gttgatccgc gtatagattt
     9541 tgagggacgt taatgtcgcg actcagcccc gtcaatcagg cccgttgggc gcgttttcgt
     9601 cataaccgtc gcggctactg gtcgttatgg attttcctcg ttttgtttgg tttgagtttg
     9661 tgttctgaac ttatcgctaa cgataaaccg ttgctggtgc gttatgacgg cagttggtat
     9721 ttcccgttgt tgaaaaacta cagcgaaagc gattttggcg gcccgctggc aagtcaggct
     9781 gattatcagg acccgtggct gaaacaacgg ctggaaaata acggctgggt actgtgggca
     9841 ccgattcgct ttggtgctac cagtatcaac tttgctaccg ataagccctt cccttctcca
     9901 ccctcccggc aaaactggct gggaacggat gcaaacggcg gcgatgtgct ggcacgcatt
     9961 ctctatggca cgcggatctc ggttctgttt ggcctgatgc tgactctctg ttccagcgtg
    10021 atgggcgtgc tggcgggggc gctacaaggc tattacggcg gtaaagtcga tctctggggg
    10081 caacgcttta ttgaagtatg gtcggggatg ccgacgctgt ttttgattat tttactttcc
    10141 agcgtcgtac agcctaactt ctggtggctg ctggcaatta ctgtcttgtt tggctggatg
    10201 agtctggtcg gcgtggtgcg ggcggagttt ttacgtactc gtaatttcga ctacattcgt
    10261 gcggcacagg cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat gttgcctaat
    10321 gccatggtcg cgaccctcac ctttttaccg tttattttat gtagttcgat aaccaccctg
    10381 acctcgctcg atttcctcgg cttcggtcta ccgctcggtt caccgtcact cggtgaactg
    10441 ctgttacaag ggaaaaataa ccttcaggcc ccgtggcttg ggatcaccgc cttcttgtcg
    10501 gtggcgatat tattgtcttt gctgatcttt attggtgaag ccgtccgcga cgcatttgat
    10561 cctaataagg cggtgtagca tgacgcaaac tctgttagcg attgaaaatt tgtcggtggg
    10621 ttttcgccat cagcaaaccg tacgtacagt agtcaatgat gtttcactac agattgaggc
    10681 tggcgaaacg ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta ccgcgctgtc
    10741 aattttacgc ctgctccctt ccccgccggt tgaatatctc tccggcgata ttcgttttca
    10801 tggcgaatcg ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg gtaataagat
    10861 cgccatgatt tttcaggaac cgatggtgtc gttaaatcca ttgcataccc tggaaaaaca
    10921 gctttatgaa gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc gtggcgaaat
    10981 tcttaactgc cttgatcgcg tcggtatccg ccaggcggca aaacggctga cagattatcc
    11041 gcatcagctc tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc tgttaacgcg
    11101 accggaatta ttaattgccg atgaaccgac caccgcactg gacgtctctg tccaggcgca
    11161 gattttacag ctgttgcgcg aactgcaagg cgagctgaat atgggcatgc tgtttattac
    11221 tcataacctc agcattgtca gaaaactggc ccaccgcgtg gcggtaatgc aaaacggtcg
    11281 ctgtgtcgag caaaataacg ccgctacgct atttgcctca cccactcatc cttacacaca
    11341 aaagctactc aacagtgaac cgtcaggcga tccggtaccg ttgccagaac ctgcctcaac
    11401 gttgctggat gttgaacagc ttcaggttgc cttccccatt cgcaaaggga ttttgaagcg
    11461 cattgtggat cataatgtgg tggtgaaaaa catcagtttt acgctacggg cgggtgaaac
    11521 actgggttta gtgggcgagt ctggttccgg gaaaagcacg acgggactgg cgctgctgcg
    11581 actgattacc tctcagggca gcatcgtctt tgacggtcag ccactgcaag atttaaatcg
    11641 ccgccagctg ttacctactc gtcatcgtat tcaggtggta tttcaggatc caaactcctc
    11701 gctcaaccca cgactcaacg ttttgcagat tattgaggaa ggcttacggg ttcaccagcc
    11761 gacgctttct gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg aagtgggatt
    11821 agatcctgaa acacgccacc gttatccggc ggagttctct ggtggtcagc gacaacgtat
    11881 tgcgattgcc agggcattaa ttcttaagcc ctcgctgatc atacttgatg aaccgacatc
    11941 atcactcgac aaaacggtac aggcgcaaat attgacgctt ttgaaatcat tgcaacaaaa
    12001 gcatcaactg gcctatttgt ttatcagcca cgatttgcac gttgtccgcg cgttatgtca
    12061 tcaggttatc gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg cgcgcgtgtt
    12121 tgccacaccg cagcaggagt atacgcgtca gttactggcg ttgagctgac gcttaaaaag
    12181 gattgtagtt tgaaaagggt tcggcaattg ccacaccaaa atttttcaat cggcatgttg
    12241 cagcaaactc gtcctggcga tttacaaaca ggcacggctc accttcacac tccaacaccg
    12301 aacatggaac ttcgatgtcg cttaacgcct ggcttagttt atacatcacc gaccatgcat
    12361 tatcaccatc cgggctaagc aatttaagcg ccaccaggct gttgtcaccg cacggtccat
    12421 tttgaggaat cggttcaacc ttcgaacctg cgaaaccagc agaccagcga tagttctgcg
    12481 gcagtcgatg gacgattgag agttgtaatg aagtcactta ttttccccgg aagtacattc
    12541 acttcacaat ttgtttacat caattttaac acatcatcaa caatccgtct ttatacagat
    12601 cgtaaagatt gatgcctcgc ctgcgtggcc tctatggctc tgatttaagt aatatctgcg
    12661 ggcgtggtca cgtttgcggg gctatatgta cccgtttctg cgatctaact caaccttttt
    12721 aactacaatg atgtgacttt ttacataaat tgattttaca taaaataaac atatatcggg
    12781 gaaatgatag atttgtggtt gacatgcatc aacaaaggaa gccttttacc ttcctcgttg
    12841 tgcaatagat caccgttttt tcggccgact ggcgtacaga cagaagagaa tggagctggt
    12901 tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag
    12961 caatgcgcca acaattgccc ctatgccaaa acggaaggtt cctgccagcg aagatgccgt
    13021 tcccgccata tggggaaact catcaagaat gaccgccatc gcattggatg acaccatcga
    13081 cacgcagccc acaaacgccg caacgccaac caccagcgac cagaatccca gccccagcgg
    13141 cgcactgatg accatccacg cagccataat aaattgtatc cacaaccccg agcggaacat
    13201 atttaacgcg ccaatgcggc ggacgaagcg gctgttaaag atggtcatca cgaacagaaa
    13261 aacaatgttt agcgcaaagt aataaccaaa gttttccggc gcgacgtggt taatttcaat
    13321 ataaacaaac ggtccggcgc ttaagaatga gaacatcccg gcaaagctga aaccactggc
    13381 aagcatgtag ctcaggacac gtttatggcg gaacagcgcc gcaaagttac caatagtggt
    13441 acgaatgtga aatggctgac gacgctccgg tggtaaggtt tctttaatca ggaagaaaat
    13501 cattgccgaa gccagaatcg ccgctaatgc caggatccag aagatgtaat gccagctcag
    13561 ccacaccagc acccagccgc caactatcgg tgccatcagc agtgcaatgg tcgtcaccag
    13621 catgacaaac gacatcatcc gcgagaactc ttctttcggg taaatatcac gcatcaaagc
    13681 gttgataacc acgctggccg cagccgcagc cagcccgtgg aagaaacgca tcacaatcag
    13741 ctgatcgatg gtttgtgcca acgcacacgc caccgcggcg gcggcaaaca ccagcgtccc
    13801 gccaagaacc accggcttac gcccgaagct gtctgccatc ggcccgtaga ttaactgccc
    13861 caacgcaaag cccagaatat aagtactgag ggtcatctgc gtactgcccg ccgatacgcc
    13921 aaactgcgct gaaattaccg gtagcgcggg cagatacata tcaatcgaca gcggcatcaa
    13981 catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc gggtggtcac
    14041 aacgggctcc tgaaagtcat tgaaaagtta gacgacgctg gcaatttctt cttcagttaa
    14101 cggacgatat tcaccggggg ctaaatcagc atccagcgta ataccgccaa tacgttcacg
    14161 atgcagctca accacgtggt tacccacggc ggcgaacatg cgtttcacct gatgataacg
    14221 cccttcgctg atggtcagac gaacctgcgt tggggtaatc acttcaagca ccgccggctt
    14281 agtgagatct ttttcgttat gcagctgcac gcctttagca aattgctctg ccgtatcgtc
    14341 agctacaggt gattccagtg tcaccagata ggtcttctcg caatgatggc gcggagaagt
    14401 aatgcggtgc gaccactgac catcatcagt catcagcacc agaccggtgg tatcgatatc
    14461 cagacgtcct gcggcgtgca gtttccacgc taccggttca tccagaaaat agagcaccgt
    14521 cgggtgatcc gggtcgtccg tggagcaaac atagccctga ggcttattga gcatgaagta
    14581 acgtggaccg tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat gttcagggag
    14641 cagtttgaac gctgcattac gaacgatttc gccatcgacg gtgacacgat tgccgcggat
    14701 ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg
    14761 catgtgtgtg attttgcctg taaaaaacgg aggtcgggta atgacccgaa aatctgaact
    14821 gttgtctgcc cagtatagcg gtctaataac ttccctcaag ggaaaaagaa ccgtggcata
    14881 ctattagcag aataatctac ctacgcgaaa ccatgatttt tacactccgc ccatatcagc
    14941 aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct gccgttatcg
    15001 tgctgcccac cggcgcaggt aaaagcctgg tgatagcgga actggcacgg ctggcgcgtg
    15061 gtcgcgtact ggtgctggca cacgttaaag aactggtggc gcaaaaccat gcaaagtatc
    15121 aggcgctggg gctggaagcc gatatttttg ccgccgggct aaagcgcaaa gagagccacg
    15181 gtaaagtggt atttggcagc gttcagtcgg tcgcccgtaa tcttgatgcc tttcagggtg
    15241 aattttcgct gttgattgtc gatgaatgcc accgtattgg tgacgatgaa gagagccagt
    15301 atcagcaaat cctcactcac ctgactaaag tgaatcccca cttacgcctg ctggggctga
    15361 ctgccacgcc ttttcgattg ggcaaaggct ggatctacca gttccattat cacggcatgg
    15421 tacgcggcga tgagaaagcc cttttccgtg actgcattta tgagctgccg ctgcgttata
    15481 tgattaaaca cggctatctg acgccgccag aacgactgga tatgccagta gtgcaatacg
    15541 atttcagccg cttgcaggca cagagtaacg ggctgttcag cgaagctgat ctcaaccgtg
    15601 agctgaaaaa acagcaacgc attacgccac atatcatcag ccagatcatg gagttcgccg
    15661 aaaagcgcaa aggggtaatg attttcgccg ccaccgttga acacgcaaaa gagattgtgg
    15721 gattactgcc tgccgaagat gctgcactga ttactggcga caaccccggc gctgagcgcg
    15781 atgtgttaat tgaagatttt aaagcccagc gttttcgcta tctggtcaac gtcgcagtac
    15841 tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat
    15901 cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg tctcgctccg ggcaagactg
    15961 attgtttaat tcttgattat gcgggtaatc ctcacgatct ctacgcgccg gaagttggta
    16021 caccgaaagg caaaagtgac aacgttccgg tacaggtttt ctgccctgcc tgcggttttg
    16081 ccaacacctt ttgggggaaa acgaccgccg acgggacatt gattgaacac tttggtcgcc
    16141 gctgtcaggg atggtttgaa gatgacgacg gtcatcgcga acagtgtgac ttccgtttcc
    16201 gttttaaaaa ttgcccgcaa tgtaacgcag aaaatgatat tgccgctcgc cgctgccgcg
    16261 aatgtgacac ggtactggtt gatccggatg atatgttaaa agcggcgcta cgactgaaag
    16321 atgcgctggt attacgctgt agcggcatgt ctttgcagca tgggcacgac gagaaaggcg
    16381 aatggttgaa aatcacctat tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc
    16441 tgcaaacgcc cgcccagcgt accgccttcg agcagctttt tatccgcccg catacgcgca
    16501 caccgggcat ccctctgcgc tggatcaccg ccgccgatat cctcgcccag caagccttac
    16561 tgcgacatcc ggattttgtc gtcgcccgca tgaaaggtca gtactggcaa gtgcgtgaaa
    16621 aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga attacgcggt taatggtagt
    16681 tttgattgat gtataaggcg attgagtata gaatctcgcc cgcttttgca tacgcaaagc
    16741 agatcactta cctgttgctg ggtcgcctgt agcaggatta atttaagaga gaaagaaatg
    16801 tttactatca acgcagaagt acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt
    16861 gccgctaaca agttcccggc aatcatctac ggtggcaaag aagcaccgct ggctatcgag
    16921 ctggatcacg acaaagtcat gaacatgcaa gctaaagctg aattctacag cgaagttcta
    16981 accatcgttg ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg
    17041 tacaaaccga agctgcagca catcgacttc gttcgcgctt aattgctgaa taagttgtaa
    17101 aaaaccccgc tccggcgggg ttttttgtat ctgcagatta tgcctgatta cggtattgct
    17161 attttttgca ggccagataa ggcgttcacg ccgcatctgg cattcagaac acaaaaccga
    17221 ttaattgccg ccagacgtcc ggcgctgcaa ttggtcgcgc aaattcggcg gcgtgccttt
    17281 aatggtcaag gtatcggttg ctggatccca gaagatgcgt tcacctaaca gcatcgcatc
    17341 aaagttaata gtcaagccac cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct
    17401 acggtctgcc gggaagctct cttccagttc gtagcctttt tcagccgcaa actccgtgaa
    17461 gctcacttcg cttacgcctg ccagctcttt cgacagcgat tccagttcga tctcttcgcc
    17521 tgcctgcagc tgctcattac agtagctata aacctgctgg cgcacgttct gtcgctctgc
    17581 tttatccagt tgtgcttcgg cagtgaaatc atcaaccgct tgcaacagcc cacggttttg
    17641 cgctttcgcg ttcaatcctt cgctggcccc gaggaaatcc ataaagaaat ccgccacttt
    17701 gcgccctact cgccctttca ggaaagtgag atagcgggtg gactctggat tggtttccca
    17761 ttcggttaaa tcgatacgcg caacaatatc cgcatgattg atatcaagat aatgggttgg
    17821 gttgatgtcg agattttcgt taacgcgcat actgctcagg ttgctcagaa ctgccaccag
    17881 caaatactcc accgccagat aacggtagtg gcaaaacagc acgaatccac cgtcagcaaa
    17941 aggatatttc gctaattcgt cacgcaggcg accggttgcc gcgcggctaa atgccagaaa
    18001 gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa
    18061 caagccgtaa gctttatttt tggcgctata gacccgatgc agttctgcca ccatctcaac
    18121 gacggtttct gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc
    18181 acgcttgata agctggtgca gagcaatctg gttgatatcc agactcatga taaactctcc
    18241 tttaagaccg ggcggtattc aaccaccgcc agttaagcga cgcaataaaa gatggcggtc
    18301 attttacaga aggcagcgta acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc
    18361 gttgctacgg taatatgttg ccctttcatc aactaactga tttcgattta tgccacaaat
    18421 ttcccgctac agtaatgaac aggttgaaca attgctcgca gagctgctca acgtactgga
    18481 aaaacataag gctccgaccg acctttccct gatggtgtta ggcaacatgg tcactaacct
    18541 tatcaacacc agcattgccc cggcccaacg ccaggcaatt gccaactctt ttgccagcgc
    18601 cttacagtcc tctatcaacg aagacaaagc gcactaaggg aaacagataa caggttatgg
    18661 taactcatcg tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg gggcactggt
    18721 ttgcactgtt caatattctg ctttcgctcg tcattggcag ccgttacctg tttatcgccg
    18781 actggccgac aacgcttgct ggtcgcattt attcctacgt aagcattatc ggccatttca
    18841 gcttcctagt gttcgccacc tacttgctga tcctcttccc gctgaccttt atcgtcggct
    18901 cccagaggct gatgaggttt ttgtccgtca ttctggcgac ggcgggaatg acgctattac
    18961 tgatcgatag cgaagtcttt actcgtttcc atctccatct taatcccatc gtctggcagt
    19021 tggttatcaa cccagacgaa aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg
    19081 tgccggttat tttattgctt gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca
    19141 gcctgacgcg tcgtcgacgc ttcgcgcgcc cgctggccgc attcttattt atcgccttta
    19201 tcgcctcgca tgtggtgtat atctgggccg atgccaactt ctatcgcccg atcaccatgc
    19261 agcgcgctaa cctgccgctt tcgtacccga tgacggcgcg acgtttcctt gagaagcatg
    19321 gcctgcttga tgcgcaggag tatcaacgcc gtcttattga gcaaggtaat ccagacgccg
    19381 tttccgtaca gtatccgtta agcgaactgc gctatcgcga tatgggcacc gggcagaatg
    19441 tgctgttgat tactgtcgat ggcctgaact actcacgctt cgagaagcag atgcctgcgc
    19501 tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc ggcaacacta
    19561 cagacaacgg catctttggc ctgttctatg gcatctcgcc gagctatatg gacggcattc
    19621 tgtcgacccg tacgcctgcg gcattaatta ctgcgcttaa tcagcaaggc tatcagctgg
    19681 ggttattctc atcagatggc tttaccagcc cgctgtatcg ccaggcattg ttgtcagatt
    19741 tctcgatgcc gagcgtacgc acccaatctg acgatcagac cgccacgcag tggatcaact
    19801 ggctgggccg ctacgcacaa gaagataacc gctggttctc gtgggtttct ttcaatggca
    19861 ctaacattga cgacagcaat cagcaggcat ttgcacggaa atatagccgg gcggcaggca
    19921 atgtcgatga ccagatcaaa cgcgtgctca atgcactgcg tgattctggc aaactggaca
    19981 atacggtggt gattatcact gccggtcggg gtattccact gagcgaagag gaagaaacct
    20041 ttgactggtc ccacggtcat ctgcaggtgc cattagtgat tcactggcca ggcacgccgg
    20101 cgcagcgtat taatgctctg actgatcata ccgatctgat gacgacgctg atgcaacgcc
    20161 tgctgcatgt cagcacacct gccagcgaat attcgcaagg tcaggatttg ttcaaccctc
    20221 aacgccgtca ttactgggtt actgctgcgg ataacgatac gctggcaatt accaccccga
    20281 aaaagacgct ggtgctgaac aataacggta aataccgcac ttacaactta cgtggtgaaa
    20341 gagtgaaaga tgaaaaacca cagttaagtt tgttattgca agtgctgaca gacgagaagc
    20401 gttttatcgc taactgatta attataaatc agttagcgaa atatcttact tgcaatcggt
    20461 gtggaaaacg gtagtattag cagccacgag tcggcacgta gcgcagcctg gtagcgcacc
    20521 gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa aatcccaaga
    20581 aaaaaccaac ccttacggtt ggttttttta tatctgcaat taattcgata aacagaccgt
    20641 gacacatcac agcctgttta ttttctgtta tcagaacgtc cagaccacac ccgcctgagt
    20701 attccacggc gattctaccc ctgcgccatt gccatagctg actgacaaat gaccgcttaa
    20761 cgtcggggta aacgatgacc ttatcccagc ctgataaacg ccactggttc cagccacatc
    20821 attattaaat ttgccatcgt cattaacttt cacctggtta gaatcggcgt attcctgacg
    20881 cacggcggct ttcagccagg gttccagcgt cgtaccgttt tgcaggtcca tgtgatagct
    20941 taccgccgtt cccgcttcag cgcgtaatat ccgggtattt cccacatccg cgcgcatgcc
    21001 gtttgataac gtgtagtcct gaccatctgt ggtaaagccg gtaaaggcca gatagggtct
    21061 aacactccac aatccgtcaa cccaacggaa cccgctctca acatgagcac ccgcgccgtt
    21121 actattgtaa tcgccaaacg ctgttgcccc attactcatc ttgccatgga tggtgttggc
    21181 aaaacggtca actttcacca ccccatcaac ataggcaccg ttctgatgct cccaaccggc
    21241 ataagccccc agggtatagc tatcgatatt accttttccg ccgcgatcaa aaccaatatc
    21301 agaatgagag taaccaaaga acaagccgcg aattgtactg ctttcttcac gggagaaacg
    21361 gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca aaaccagctc ccgcatcagt
    21421 ggtcacgttg ttgcgggtgt taattgccga actccacatc accgtatcgt aactaacgcc
    21481 ttttacgcta ccaagacgct cacgcacggt gtccagttct gcatcaaata ccagcggttg
    21541 tgcggctgcc atgttcagca catcagtggt tgaaggggta atttgcgcgc gattctctgc
    21601 gaggctccag ctatggttgc cattatccag caaggtatat tcatacgtac cgatatcaac
    21661 aacgcctccg gcattgccca acgtaaatgc agcatcaccg ccgcccgttg ttaccagtgt
    21721 aaggctatct cctgctgccg ggctggcacc ggtgtccgtc acgaatattt tgaaatcacc
    21781 tgttgcctga ccggtgacgt tgagctgatc gctctgatga ttggccatat cggtacgcat
    21841 ataaaaattg ccgtttcccg acagggtatt ggtggtcaac gtaatgaaat tttccgcact
    21901 tgccgtcaca gatgaacgag tcactggctc agccatcata atcacatttg cgccggacat
    21961 cgcaaggttg gctaagttga ggcgagtgtt ttggttcgtc gtagtgatat cggcaatgat
    22021 ggtccatgcg ctattttcga gcgaaacgtc cgctttgctg gtatccacgg caccatgcgt
    22081 tctaaaagaa gcgccctcag aaaccactac cttacccacc aacatagcgg gtgcataatt
    22141 tatttgcggc ttcatgacct caagaatgcc atcattccct ctgactgaca cgttaaccat
    22201 tgtgccagcc cagacattag cgcggccatt gttgattacc atattttcag agatggcttt
    22261 tgaggaatac gtgatacttc cattctggtc tgatcgaccg agatcatata ccgcatttgc
    22321 ctgcactttc gtaccagtat cctttcccag cgattgcata gtggcatgct catcaaggat
    22381 agtgtcaatg gcctgcgtgt cctccataac aatgagcccg gaaccatcat ccagttcata
    22441 attttttgac acaccatcct ttatactaaa ttggccttta ctgtttgtac cactcacttc
    22501 cagcgcattc gttgagacaa ttaatttccc gcctgctttt ttatcaacgc cagttgcagt
    22561 cccgccatcc ataacctcca gtaagccgcc actatccaca gtagtattat aagcgaagtc
    22621 attttcttca actcgcaaac ttccgccgtt ttccaacaac atattattag caataccatt
    22681 cttgatatcg aactgaccga gacggtttgt tccgaatacc tccatggccc gcgtggttgt
    22741 tttaatagca ccgcctgttt tctgatctac cgcaagcgca aatcccccct ctttaacctc
    22801 cagtagtcct cccttttcga tagcggcaaa aacggccccc ccatcttttt caacgattaa
    22861 gcgcccgcct ttaatcgtgg tatcattcgc tgggccataa acctgtagca caccattatt
    22921 gagggtagtc tggtttgccg cgccacccat ttgaacaatt tgataaccat catttaatac
    22981 tgagtattct gcggtaccat tcatttcaat gtattgatac cctccgttaa tttcagtatt
    23041 actacttaca ccaaattctt ttatatgctg ttctccgcca ggattgattt tcgtattatt
    23101 ggcggtccca taaaccagct ggatatcttt ttccaggata acaccatcaa ctgtctcacc
    23161 atactcaact gttgatgcca atgctatggg tgaatataac gctgtagcca ccagaacaga
    23221 tattgcggaa cgacaaagag aaacagaacc agattgatgc attgagcttt catcctatga
    23281 aattaattgc agttaaaagc attgggtaca gaaaataccc atagctccat acccggagtc
    23341 agtttttaaa aactgtttaa agaaatgcac aagtattgtg attgattttt tagttgtttt
    23401 tcttgatgag aagctgatgc aaaattccgt ctttataata aaaatgatgc caaagcgaac
    23461 gacaaggttg tagttttcac tacatgtcca tacataaaat ggggtaacat tcacgcgcct
    23521 ggtagcgtta ccaacgctac gctcaaacat aatgattcta ataaaacctc aggagactac
    23581 tatgcctgaa gcaacgcctt ttcaggtgat gattgtggat gatcatccac ttatgcgacg
    23641 cggtgttcgt cagttactgg agcttgatcc tggctttgaa gtggtcgccg aagcgggcga
    23701 aggcgcgagc gcgatcgatc tggcgaatag actggatatc gacgtgatct tgctggatct
    23761 caatatgaaa ggtatgagtg gcctggatac cctcaatgcc ttgcgcaggg atggcgttac
    23821 cgcgcaaatt attatcctga ccgtatccga tgcctccagc gatgtctttg cgctgataga
    23881 cgcaggcgca gacggttatc tgttgaaaga cagcgacccg gaagtattgc tggaagcgat
    23941 tcgtaccgga gcgaaaggca gcaaagtctt tagcgaacgc gtcaatcagt acttacgtga
    24001 acgtgaaatg tttggcgcgg aagaagatcc cttcagcgtg ctgacggagc gcgagctgga
    24061 tgttctgcac gagctggcac aggggctgtc aaataaacag attgcatcgg tgttgaatat
    24121 ttccgagcag acagtaaaag tacatattcg caatctgctg cgtaaactca atgtccgctc
    24181 acgcgtggcg gccaccattc tgttcctgca acaacgcggg gcacaataaa aatagcccga
    24241 tggatttatc atcgggctga gatttatgac aaacgcaaaa ctgcctgatg cgctacgctt
    24301 atcaggccta cgtggatcga tcaatttatt gaatttacac aattttgtag gccggataag
    24361 gcgttcacgc cgcatccggc ataaactaag cgcactttgt catcagtcta gcccgatggc
    24421 atcaccatcg ggcctctttt tatttactct cctgcggcga caaatgttgc atcgcctgcg
    24481 cgatactacg ttcaatcacc gcacggcgag tatcgttggc aggtaagagt ttcaacatca
    24541 tctcccacgc ggcaacggct tcgccaaatc gctgctgctc aaaggcatta aacgcataca
    24601 tgcttagcac acggatattg ctatggtccg ttctcaccag ctgacgtagc agttcaccgc
    24661 cgaggcggtt gtcgttggga tcagatgaac gagtcaacgc ttcagcgtat cccagtgcgg
    24721 catcgctgtt cttcggatcg aggcgatacg cagtagcgta tgcatcggtg gcgatactgg
    24781 cgttacccag cgccatgcca acgcggccca acataatcca gccttctata tctcccggat
    24841 ttttttgcag ttgagtacgc atccccagcg caaggcgcga catctcttct tcgttgagcg
    24901 gatcggcttt cgggtccagc gccctgtcca gcaacgccgg agcctgtgcc gtggcctgct
    24961 gccagatttt cacctgctga taattgccag tctggtagta gctgacgcca gccacaatta
    25021 acgccaccac aatacccggc agataaacaa catatccggc acgcttacct tccggcacgc
    25081 tttgttcagg aaacgcttcc ggcaccacgc gtacccgacg ccgcgagcgg gcgtatatga
    25141 cccaaccgcc aatgccaata gccactactg gcagcaccca cagcagcacg gtcagcggcg
    25201 ttaacggcgg atcgtaagtg acgaagttgc cgtaacgcgc caccatataa tcgacaatct
    25261 cttttttact tttaccttcc tgcatcagtt catacacttt ctgacgcagg tcggtggcaa
    25321 tcatcgagtt ggaatcggca atgctgttgt tctggcattt cgggcagcgc agttcttcag
    25381 tgagctgacg gaactgttgt tcctgtgctt catctttaaa ctgcaacacg tcgatggtcg
    25441 ccagcgctga gccggagatc atcagcatca gcacgcccaa taaaaacctc attgtgcggc
    25501 ctccttactg tatttctccc acagcggctt gatctcttct tcccagacgc gaggattgag
    25561 atcgcccgca tggcgatagc gaatgatacc gttgccgtca ataaggaagg tttctggcgc
    25621 gccatagaca ccgagatcca gccctaacat gccgtcgcca tcaaacaggc ttagcgcgta
    25681 aggattgccc agctctttca gccagctgat tgccttctgg cgatcgtctt tatagttcat
    25741 gccgaccacg cgaatgccct gcgctgaaag ctgattcaga tattgatgtt ccgcacggca
    25801 ggtcggacac caagtcgccc agacgttaag cagtactggt ttgccctgag tcagcacatc
    25861 cgcctgataa aactgccccg gattgtccag tgattcgaga cgaaacttcg gcacaggctt
    25921 gccaatgagc gccgattcca gattggtcgg atcatcccct tcggcattac gcgccagctg
    25981 ccacagcagc gccgcggcaa tcgccaggaa gataatcaac ggaattaaca atactttgcg
    26041 cttcatacgg cctccggcgc agttttttgc ggactcacgc gcttacgata gcgaggatca
    26101 aacagacaca gcagtccgcc caacgccatc atcagcccgc ccgcccaaat ccagcgaaca
    26161 aatggtttgt agtaaagacg cacggcccac gcgccgtttt ccagctcttc accgagcgcg
    26221 gcgtacaggt cacgcgtgat gccgccgtca attgccgctt cggtcatcat cgacccggca
    26281 gtgttgtaat aacgtttttc cgcatacagc accgtttccg gtttaccctc tcgcgttacg
    26341 ccaatagtcg ccacaccgcc gcgccagttc gggccagtca cctctttgac atcacggaag
    26401 gtgaagcgat attcatgaat atcgacgcta tcgccggact tcatgcgcac atcacgctca
    26461 acgctataat tctggctaaa ggcaatgcca acaattgtca ctgccagccc caggtgagcc
    26521 gccaccatcc cccaataact gaaggtggtt ttcgtgccgc gtgaaatacg cagcgcagct
    26581 tccgcagttg ccagcaccgc aatccagcag gccattgcca ggccgagcac cgtcatcgcc
    26641 acaactttgc tttcgaacag ccacggcaac agcagagaca gcaccagcgt agagatgaag
    26701 gcgataatca ataaattgcg gattttacgt gggcgatcgc gcccccagcg caccagagga
    26761 ccgacaccaa gcagtagcgc aaacggcacc atcagccagg taaacatggt gttgaagaac
    26821 ggttcgccaa tcgaaatact gcccagtcca agttgcttgt gcaccagcgg cagcagcgtc
    26881 cccagcaaca ccaccagcat cgcggcgacc agcaaaacgt tgttcgctaa cagcaaagat
    26941 tcccgcgacc acagcgcatt gtttacgcgt gagcgaactt tgtgtccacg cgcggcaaac
    27001 agcagcagcg aaccgccaat caccagcacc ataaaggcga ggataaacat accgcgcgcc
    27061 ggatcagacg caaacgcgtg taccgatacc agcacgccgg aacgcacgag gaaagtcccc
    27121 aacagacaca acgagaaggc actgattgcc agcagtaatg tccacgcttt gaagctggcg
    27181 cgttgttcag tgaccgccag tgagtgcatc agtgcagtcc ccaccagcca cggcataaac
    27241 gaggcgtttt ctaccggatc ccagaaccac cagccacccc agccgagttc gtaataggcc
    27301 catgcggaac cgagcacaat gccgagcgtt aggaaaatcc acgccgccag cgtccacggg
    27361 cgagtaaaac gcgcataagt gctgtccaga cgcccgctca gcaaagaagc aatggcaaaa
    27421 gcaaacgcca ccgagaaacc cacgtacccc atataaagca gaggcggatg gaagatcagc
    27481 cccggatcct gtaacagcgg gttaagatcg cgcccttcaa tcgggaagtt cggcagcgtg
    27541 cgtgagaacg ggttagaggt aaagagaatg aacagcaaaa agccgacgct gaccatcccc
    27601 attatcgcca gtacacgggc cacaatatcc agcggaatac gctgactaaa aatcgccacc
    27661 gcaaaggtcc agccgctcat cagcagcacc cacagcagaa gcgaaccttc atgcgcgccc
    27721 caagtagccg ccacgcgata ccacaccgga agctgggtat tggagttgct ggcaacatag
    27781 gtgacggtaa agtcgttgac cacgaaagca ttgaccagca ccagaaatgc gccagccaca
    27841 gacataaaca gcagccaggc aaacaagcgg gaagacgcca tcatgcgcgc atctccgcgc
    27901 gccacgcccc atagcggata cacggacagc agcagcgcaa tccctaacgc caagcacagc
    27961 agcccgtttc caatttccgg catcatgatg ctgggtcctt ataaacactc gccgggcgac
    28021 ggtggttagc ttccatcgct ttctcaactt ctggcggcgt gtagttttcg tcgtgtttcg
    28081 ccagcacttc tttcgcgagg atatgattgc ctttttccag ctcgccctgc accacaacgc
    28141 cctgcccttc acggaacaga tccggcaaaa tgccttcgta agagacatcc actgagcctt
    28201 cagcatcgta aatggtgaag gtcactttca gcgaattggg atcgcgctgc acgctacccg
    28261 gcatcaccat cccgccaacg cgcagacgct gaccgacttc cggcatctgc tgagtttcac
    28321 gcttgccgta gagaatttcc cccggcgtat aaaagagatc gatattcgag cgcagcgcat
    28381 atagcaccag accgatagtc agcgctagcc ctgccaacac ggcacaggca atccacaagc
    28441 ggtttttacg gcgaatattc atgcagcctc ctgctgttgc gcagcacgta aacgcgcctc
    28501 acgcgcccgc tgttgcgcca caccacgcag aattgcgcga tgttgcatca ccgagtgcac
    28561 gaccaaaacc accagcggaa taacggtcat caccaccgcc agccagacaa aaaaggcgta
    28621 accgcccatt gcgaaaaatt cattccagga agcaaatgca ggggtcattt acggcctctt
    28681 ttcagtatca gttcactcac ccacggacgg cgtttttcca tcagcaaaat caaattacgc
    28741 atccgcatca gcgtcagcgt ggcagacagg agcaggaagc caaaaatcga ccagcgcagc
    28801 ggcgaacgca tcgccggatc gatactttgc tgcatccgcg ttgatccctg atgcagggtg
    28861 ttccaccact ccacggagta atgaataatc ggcagattca ccacgccaat cagcaccatg
    28921 atacctgccg cacggcccgc cagacggcgg tcgtcgaagg cgtgccacag ggcaatcaca
    28981 cccacataca aaaacagcag caccagttca gaagtcagac gtgcatccca tacccaccag
    29041 gtgccccaca tcggttttcc ccatgcagag ccggtaacca gggcaataaa ggtaaacacg
    29101 gcaccaatgg gggccatcgc cgccaccgcc aggttggcca ttttcatctg ccagacaagg
    29161 ccaataaacg ccgccaccgc cattgatgca taaatgccca tcgaccagat cgccgcaggc
    29221 acatgcaggt agataatgcg gtagctattt ccctgctgat aatcagccgg agcaaagccg
    29281 aatccccaga tccagccgac ggtaagcacg accacactgg caattgccag ccacggtata
    29341 aaccagccac agatttgata cagccgtggt gggatcgcca gttgatgcag tgttttccac
    29401 atagtttcga taccagactc gaacaaaaat cagtaatcca gcgttattga atgctgattc
    29461 gtaacgctgc cgccgtcgca aaaggactta atgtcgcggt gcctgccagc aacgcgccta
    29521 aaattgccag atacccatca acgggcaaat gcatagaagc cgcgtccatc gcggcggtgg
    29581 caaagatgag taatgggata gtcagcggta acaccagtat gctgagcagc acaccaccgc
    29641 gcttaagtcc cactgtcagc gccacgcccg gtgcaccgag aaagccaagc gtaggcgttc
    29701 ccagcagcag cgtcagcgcc atcacttgcc agccataaac atccattccc agtagcattg
    29761 ctaccagtgg cgaaaggatg agtaacggca gaccggttac catccagtgc gccatcacct
    29821 tcgccagcac aacggcgggc aagggtaacg gcaacaacat caattgttca agactgccgt
    29881 cctgcaaatc gtcacggaac agtcgttcca gcgccagcaa ggatgaaagc agcgcagcaa
    29941 cccagataat gcccggtgca atacgcgcca gcagttgcgg ctccggaccg atactgagcg
    30001 gaaaaagggt aattacaatc aggaagaacc acagcgggtt ggcgatttcg gcgctatggc
    30061 gaaacgctac gcgcagttca agacggaaaa tacgccagaa catcatgcgg ccctcgtttg
    30121 cgtcagtgaa atgcggcgaa ttttactttc agcaacgttg agcggctggt gggtagtcag
    30181 aatcacaatc cccccctgct ccgtatgctg cgccatacgc tgggtcagac gatcgacgcc
    30241 gttaacgtca atcgcggtaa aaggctcgtc gaggatccat aatgtggcac gggtcaacca
    30301 cagacgcgct aaagcgacgc ggcgttgttg cccggcagaa agttgattta ccggaatatc
    30361 ttcgaatccg gcaagcccgg cctgcgccaa ggcttccaga cattgtgcgg catcgccatc
    30421 gcgatgataa aagtgcagat tttctaacgc cgtcagccgg gttttgatcc ccggctgatg
    30481 gcctatccat aacagggttt gatggtagct gtcgcgtacc tgatgcaagg gctgcccttg
    30541 ccagagaacc tcgccagcgt cagggcgaga caaccccgtc agcaaacgga gaagcgttgt
    30601 cttccccgcg ccgttgctac cggtgatttg tacccactct cctgcgttca gcgtaaatga
    30661 caagccacta aataaggttc gttcatcccg ctcacaaagt aactctctgg cttcaagcat
    30721 acccacgcaa taaccctgtt aaaaacctgg ctcgacttca cgcatatcgg gcagcttgtg
    30781 cgcgatccct ttatggcaat caatacaggt ttgcccatct ttcaccgcct ggtcatgcat
    30841 cttcgcggca accgatttct gggcggttgt atccatatac tcgaagttgt gacagttacg
    30901 gcactcctgc gagttattgt ccttcatgcg ccgccactca ttctgtgcca tcgtcagacg
    30961 atgagcttca aatttctgcg gcgtgtcaat aacgccaaaa attttaccat acagctcttt
    31021 acttgctttg agcttgcgta tcatcttcgg cacaaactcg tgcggaacgt gacaatccgg
    31081 acaggtcgca cggacgccgc tacggttgtt gtagtgcacg gaatccatgt attcctgata
    31141 caccgtgttg cgcatttcgt ggcagctaat gcagaactct tcggtattgg ctttttccat
    31201 cccggtgtta aagccccccc agaagacgat gccgccaaca aaaccgatca acagcagcgt
    31261 ccccagcgcc agatggctgg gggtacgcca ccatttccac aggcgcttaa tcagaccagg
    31321 cttacggtca gaatttccca taataacctc ttatttcccg taaccttttg atggggtaaa
    31381 ggtattcccc acgattggcg cggtatcggc ctgcggtacg tgacattgca gacagaaata
    31441 acgacgcgga gccacttccg cgcccacttt gccgtcgctg tccataaagt gagtaggact
    31501 gatacgcggc gcgccagtgg tgcgatagct ttcgacaccg tggcactgca agcagcgatt
    31561 ggtattggtc gttacctgat aaccttcaac gctatgcggg atcattggcg gctgattcac
    31621 atagttcagc ggcatccgat cctgctcttt tggcatccga atggcccctt cctgcgtccc
    31681 ggatacttcc ggcgactggc taaaatcgac gccattagcc gcccaaaccg cgccgcttac
    31741 caccagggcc agcatcgccg tccattgaca cagcgctttc ttcaggtcat ggcttttcat
    31801 gatttcgctc ccgaactcca tcgtgtagtt attgtaaata catcctcaga acagacatcc
    31861 acgcagcgac cgcacgtcat gcaatcgcgg ctggtgacct gcaccgggct ttgttcatcc
    31921 agcaccgggg cacgtagcac atgcggttcc gggcaaacat gaaaacaatc catacagcgg
    31981 ttacatttct gccgatcgct ggccgcaacg gtaatcacac ctttgctacc cagcacgcca
    32041 tacagcgcgc caaccggaca aatgtgcccg caccagccgt gttcaacgac cagtagatca
    32101 aataaaaaca gcgcgagaat aagcagcgcg ccgctgccga agcccattac caggctacgc
    32161 cccatcagag aaacggggtt gatccattcc caaatgagcg tgccagttaa ggctgatccc
    32221 accagaatga ccaccaacag cacgtagcga atgtggcgag ggatcgtcgc agactgattg
    32281 aggtcaaagc gcctgcgtaa ccagttcgct aagtcggtaa tcgggttcag cgggcagacc
    32341 cagctgcaaa ataatcgctt tcccgccagg gcgtagagca cggtgataat caccgcaccg
    32401 gtcaacgcca ccgtggcagg cagatgacca ctggcaagac tttgcagcgt catcagcgga
    32461 tcggtcagcg gaacggtgtc gaataacagg ctactgctgt agttgccgtg caagatccac
    32521 acaccaaacc atggaccgct caaaaacatc cccagcacga agaactgaca aaggcgacgt
    32581 aacaccagcc aacggtgact gcgccaccag ccttttttct ccagcgcctc gcgcccggcg
    32641 tcacgtttac gatttgccat tgttcccctc cagccagccg aagcggtaat ggtgacctaa
    32701 ctcccctttc gccagtgaca gcggtaacac cttgattgcc ggttgttcca gcacgcatac
    32761 tttttcgcac ttaccgcaac cggtacaggc gtcgctgtga accgtcggca gaaagcgggc
    32821 gtgcttaccg gtacgcgtgt tgcgctccag ctccaaggtg atggcctcat caattttcgg
    32881 gcattcgcga taacaaacat cgcagcgcag cccctgaaag ttgagacagt tttcctggtc
    32941 caccagtacc gccagcccca tccgcgcgtc gtcgatcgat tcaatctcac gatccagcgc
    33001 accgcttggg cacactttgg cgcacggaat gtcctcacac atttcgcaag gaatatcccg
    33061 tgcgacaaaa tatggcgtgc ccgccgacag accagaggcc agcgtcgcca gttttaaggt
    33121 gtcgtaaggg caagcctgaa cacactgacc gcaacgcaca caggcactgg caaaggcgtt
    33181 ctcgtttatg gctccgggcg ggcgcaaccg cacgccagat gcgcgtgcgg tttgctgttg
    33241 taaccccagc gccacaccca cggcagccag cccgcctgct gtgcgaacga catcgcgcag
    33301 aaagcggcgg cgaccatttt gaggtttcgc tgaccgggac ataatggctt acaccttctc
    33361 cagtttgacc gcgcacttct tgaagtccgt ctctttcgag agcggatcgg tcgcatccag
    33421 cgtcagtttg ttaaccagct gtgcggcgtc gaagaacggc atgtacacca ggccctgtgg
    33481 cggacggtta cgaccgcgcg tttcaacaat cgagatcact tcgccacggc gagaaaccac
    33541 tttcactttg tcgccacggc gcagatcgcg cgctttcgca tccagcgggt gaataaacaa
    33601 gaccgcttcc gggaaggcgc ggtgcagttc cggcacacgg cgagtcatac tgccggtgtg
    33661 ccagtgctcc agaacgcgtc cggtagagag ccacaggtcg tactcttcat ccggtgcttc
    33721 cgccgccggt tcgaacggca gtgcgaagat caccgctttg ccatccggtt taccgtagaa
    33781 cttatagcct tcgcccgctt tcacgtacgg gtcgttacct tcgctgtaac gccactgtgt
    33841 ttctttacca ttcaccaccg gccagcgcag accgcgcgct ttgtggtagt catcgaacgg
    33901 tgccagatcg tgaccgtgac cgcgaccaaa ccatgcgtac tcttcgaaca gccctttttg
    33961 cagatagaag cccagctcgc gggattcatc gttcagctga tcttccgcca gttcggatac
    34021 cgggaatttg ctcacttcgg gggtggcata cagaacttcg tacagcgttt tgccacgcag
    34081 ttccggtttc ttcgccagca gctcttccgg ccatacttct tcagttttga agcggcggga
    34141 gaactggact aactgccaga gatccgattt cgcttcgcct ggcgcctgta cctgctgacg
    34201 ccagaactga gtacggcgtt cggcgttacc gtaagcgccc tctttctcta cccacattgc
    34261 ggtcggcagg atcaagtcgg cggccagcgc actgactgtc ggatacggat cggagacgat
    34321 gatgaagttg cgcggatcgc gccagcccgg catacgctct tcattgatgt tcggcccggc
    34381 ctgcatgttg ttggtacaca tggtccagta aacattcagc ttgccgtctt tcagcgcacg
    34441 gtcttgtgcc accgcatgaa gaccgatttt cgccggaatg gtgccgctcg ggatattcca
    34501 cttcttctca cagatatcac gatgtttctc gttagttacc accatgtccg caggcagacg
    34561 gtgagcaaag gtgcccactt cacgcgcagt accacacgca gaaggttgcc cggtcaggga
    34621 gaacggaccg caacccggct gggaaatttt gccggtcagc aggtgcaggt tgtagaccag
    34681 gttgttagcc cacacaccac gggtatgctg gttgaagccc atcgtccagt aggagatgac
    34741 tttcttgttc ggatcggcat acagctgcgc cagttgttct aactggtctt tcggcacgcc
    34801 ggtcatttca gcggtttttt ccagcgtata ctcagcaacg aaggctttgt aatcttcaaa
    34861 gctcatcggt tcggaggcgt cagaacccgg attcttcgct gctttttcca gcggatgggt
    34921 cggacgtaaa ccgtagccga tgtccgtcgc ccctttgcgc aggttaacgt gcttgctgaa
    34981 gaagtcctga tttatcgcat tgttttgaat gatatagttg gcgatgtagt tcaggatcac
    35041 caggtcagat tgcggcgtaa agatgatgcc gttatccgcc agctcgaagc tacgatgctg
    35101 gtaggtagaa agcaccgcca cggtgacgtt ctggttagag agacgactgt tagtgatgcg
    35161 tgaccagagg atcgggtgca tctccgccat gttagcgccc cacagcacaa acgcgtcagc
    35221 ctgctcgatg tcgtcatagc agcccatcgg ctcatccata ccaaaggtac gcataaagcc
    35281 aactactgcc gacgccatac agtgacgtgc gttcgggtcg atgttgttcg aacggaagcc
    35341 cgctttgaac agcttggacg cggcataacc ttcccagata gtccactgac cagaaccgaa
    35401 cataccgatc gattccggcc ctttttcttt cagggcggtt ttgaatttct cttccatcac
    35461 atcgaaggcc tgatcccagg tgattggggt aaattcgcct tctttgtcat atttaccgtt
    35521 tttcatacgc agcagcggct gcgtcaaacg gtctttaccg tacatgatct tgggcaggaa
    35581 atagccctta atgcagttca ggccacggtt aaccggtgcg tccgggtcgc cctgacaggc
    35641 caccacacgc ccctgctgcg ttccgaccag aacgccgcaa ccagtaccgc agaaacggca
    35701 cggcgcttta tcccatttaa tggcttcctg ctgaccaaca acggcgcggg caacgcccgg
    35761 cacgctgaga ccggcagccg ccgcagcggc cgcaacggcg ttagctttca taaagctacg
    35821 acgactgagt ttcatggtgt ttcctcacct tgctcttcct gctggtgata aaccagcgac
    35881 accgccagca cgccctctac gttgcgtact gactcaatgg tttggatcag cgtttcgctg
    35941 tcttctgctt ccaccaccac aatcaactga ccgctcggcg cgtcgctgac agcaacttca
    36001 cagccgggaa aggcgttcag ttgggtgctg atgtctgaaa ttcgttcgct tttggcctgc
    36061 acgaccaggc tgcaaacttg ccagttagtg tgcatggaga tactccgcag ttatggctga
    36121 taccggacag ctggctgcac atgctccaca tccgttgcaa agttggctat taagttgcgc
    36181 ctggtagatc ccggagagcg tcgggcgaaa gataattgcc attggctcac agctatcctg
    36241 acagcggcgg cattcaactg actgatacgc caggcacgcg tccccgatgg tgaactgcaa
    36301 atcccaggcc ctggtgtggc gcggagaaaa tagcgattcg gggcaagcct gcgcgcaggc
    36361 gtaacagaag ctgcactcgt tatttttgaa attaacgctc ggatagccac ccgtgccgcg
    36421 ttgcagaatg ttgttttcac aagcattaat acaggcgtcg cagcgggtgc aatgggtcag
    36481 aaaatgagat tcatcaccgg accagggcgg acggataccg ttactggctt tgcgccagca
    36541 accagtgagt atgccccgac gggatgcatc aatcttcaca ttgaccttcc atcattaacg
    36601 cgctctgaaa attgagagcg accaaataga ccgcataatt aataagccat ttttatagcc
    36661 gctaagatat taaaggatgt gtcaaagatg cataccccga tcggggtaaa acctgtagca
    36721 ggatcaaaag agtggcggga agcgtggcaa aaacgggctt ttgctcacat ttcaaatggt
    36781 tataaacata tttatatagc gattaattca ccagagatat ttctgctggt ttgctccctc
    36841 atcagaattt aacactaaaa gagcaggtaa aattgtctga atgttcttta agttattcat
    36901 aaagcaaatt aataaatctg atgaatatgt taaccttcag cgacatcatc ggtgaaaacc
    36961 tataaatgaa gaaggaaagc aaaaaaatga agaccattct acctgaagta ttgtttgccg
    37021 ctttcgctac cacttccgcc tgggcggcag aaagcgtcca gccactggaa aaaatcgcgc
    37081 cttatccaca agctgaaaca ggcatgaagc gtcaggtgat tcagttaacc ccgcaagaag
    37141 atgaatctac cctgaaagta gaactgttaa tcggtcagac gctggaagtc gattgcaatt
    37201 tgcatcgtct cggcgggaag ctggaaagca aaacgctgga aggctggggc tatgattatt
    37261 atgtctttga taaagttagc tcgccggttt ccacgatgat ggcctgcccg gatggcaaga
    37321 aagagaagaa atttgtcacc gcgtatctgg gcgatgctgg aatgctgcgt tacaacagca
    37381 agctgccgat cgtggtgtat acgccagaca atgtagatgt gaaataccgc gtctggaagg
    37441 cggaagagaa aattgacaac gcggaagttc gctaaactgt tgtgaagtac ggcaccccgt
    37501 tggttggata aggcggttac gccgcatcca acatctaacg cccgagccgg ttgcctgatg
    37561 cgacgctggc gcgtcttatc agtatacgcc acatcattac aacgcaatat ccgccacttc
    37621 tttttgaacg ggttgcggtt tcaactgcgg tttcggcgta ctatctgctg cttgcggttt
    37681 gtcgtatttc agccccagca cttcgctggt gtactgcaac tcgcgttctg ttgccgctac
    37741 atcaccgttc agcttgcgtc cataagacgg aacgatcgct ttcaacgtag cctgccattg
    37801 cgggctggaa acgcgatcgc caaatacttt ttccagtaga ttcaacataa tcggcgcggc
    37861 ggttgacgcc cctggcgatg cccccagcag cgccgcaatg gtcccttgct ggtcactgac
    37921 cacttcggta cccagacgca gtacgccacc tttctcggca tcacgcttga taatctgcac
    37981 acgctgcccc gcttgccaca aacgccagtc ctcttttttc gcttgcggat agtactcttt
    38041 caacgcttca aaacgatcct cttcactcaa catcacctga ctcaccagat atttcaccag
    38101 atcgaaatta tccagcccga cgtgcatcat cggcatcacg ttagaggtgg tggtggaact
    38161 catcagatcc cacaatgaac cgtttttgag gaatttggtt gagaaggtgg caaatggccc
    38221 aaacagcact acgcgtttac cgtccagaac acgggtatcg atatgcggaa ccgacatcgg
    38281 tggtgcgcca acggatgctt taccgtaaac tttcgccaga tggcgattaa ccacgtctgg
    38341 attttccgaa acaaggaact gtccgcccac cgggaaaccg gcgtagtctt tcgcttccgg
    38401 aatccccgat tcctgtaaca gcttcagcgc cgcaccgccc gcgccgataa agacaaattt
    38461 ggcgcggatg ttctgtgcag tgccgttttt cagatcggca acggtaacgg tccaggtgtt
    38521 atcgtcatta cgctttaggg cgcggacttc gctgctgagt tgcagcgaga agttagattt
    38581 cttctgtaag gaagcaatta actggcgggt gatctcacca tagttcacat cagtgccaat
    38641 ttctgttgcg gatcgcgccc ttccatcact aacggtgccc actctttgat ctgcgcgtga
    38701 tcttcagagt aacgcatacc gcgaaacagc gagctttgtt gcaacgcggc gtaacgggcg
    38761 cgcaggaaat taacgttatc ctcgccccag acaaagctca tatgcggaac ggtattgata
    38821 aatgaacgcg gagtacgcag cacgccgcgc tcgacctggt gcgcccagaa ctggcgggaa
    38881 atctgaaatg cttcgttaat ggcgactgct ttttcaatac tgatgctgcc atcggcgttt
    38941 tgcggggtgt agttcagttc catcagtgca gaatgcccgg ttccggcgtt gttctagccg
    39001 ttcgaactct cctgcgcgcc cccctccagg cgctccacca tggtcatcga ccattcaggc
    39061 tccagctcgc gtcaataggt ccccaacgtg gcgctcataa tgccgccgcc aatcaacagt
    39121 acatcagttt cctgctcctc ggacgctttc gcttttgccg ccatcgaaac ggcattaagc
    39181 cccacggcca tcgagaagag catggcagtc acttttttca tcttgttaat gccttacttt
    39241 tagtcgcttt attgcaggtg agatttgcgc ggcatcaacg gtaacactta agtaacaaca
    39301 tttaaataat gtttataaat tatattctaa ttttagaaac attataattt cgttgattaa
    39361 ttatagggtt attagaaatg aagggatttt tagcatattg acctgacggc agcagtccgc
    39421 caggtcagcg tcgtaatatt atgccgtccg ggcaacggca tcacgcgaag cggcatcgcg
    39481 ctcttcgccc gtcagctcgc taagttgccc attgcgcatt tccagcaggc ggtcggcgtg
    39541 gataaagtaa tgatcatcat gactgatagc gaaaatagtt ttacccatct cctgcatcag
    39601 cggcagcaac acctgataaa actcacgacg gaagtgtgga tcctgatcca ccgcccattc
    39661 atccagcagg ataatatcgc gttcttctgc cagcgccagc aacagcgcca cgcgtttttt
    39721 ctgccctttt gataacttca ggttaacaat acgcccgttg cttaactcaa gcttatgagc
    39781 cattttcagc tgcgccagcc acttctcaac cagttgcggg ttagcgggtt gcccctccgg
    39841 ccccagcagt tgatcaaaca gccagacatc ggtaaacact gccgaaaaca gtttgcgata
    39901 atcttccggt tgttcgccgc tgacaggttt gccatccagc aagatttcgc cgctttgtgg
    39961 ctgatacaag cccgtcaaca acatcgccag cgtcgatttt ccgctaccgt taccgccaat
    40021 cagaaacagc agctcgccac gtttgatggt gagattaatc ggaccaacgg aaaacgcgtt
    40081 atcctgataa gcaaacgtca cgttacgcag ctccagcgtt tgccagttgg gaaacgcctg
    40141 cgggcgcgga aactcagctt tgaaaggcgc gagcgcgaat ttgttcagct tgttaaacgc
    40201 cacctgcgcc gtcagcagcg tcggcaatgc gccaaccgcc gaaagcagcg gcgtacgcag
    40261 gaataacagc gtcaacgaat aggtcgcagc aacgttggta tcagcccaac cgagactgtt
    40321 cgccatccag aacaccaggc cgatagcgcc cagcatcatg atgtttgacc agttcacggc
    40381 actaagatgg aaggtgtctg cgcgaataat atgatggcga tactcttgcg catcggggat
    40441 gtagaggttg ttaaacacat actcggcgcg ttcccggttc agagtcagct ctttgcgccc
    40501 ttcaagtaca gtttgaaaat ccgtgtacag cttgtcttcg gtttcacgta gggtcgccat
    40561 atgtttgtac acccgcgcca ccagcacaaa accgccccag atggtgatcg ccatccagat
    40621 agccgttacc agcaacattt tgcccgacag catccacaga tacgccgatg aaccgatagt
    40681 gagaatgatc ccctgcccca gttccggcag acgcacaaaa gcaatggtga tattgcgcac
    40741 atcgctggtt aatcccgcca gcaacgaggc gctaccgagt tgttcaatgc gctcgacgtg
    40801 ggtatccaga atccgcttga taaattcact acgcagtcgg taaacgaagt gatgccccaa
    40861 agtggtgagc gccagttgcg atccgagagt gactgccatc aacagcagca ataatcccag
    40921 aaactccggt aacaccagca gactggtatc cgccgtttcg ataaggcgct gattgataaa
    40981 agcaattaag ccaatgccta atgccgcact ggcgaggctt agcgccatca cactgataaa
    41041 tggccagcga tactgccgcc agacaagtac aagaagttcc atgcagaaaa cccggacaat
    41101 gaattacagc ccgcagttta aacatcttcg cgcgcacagc aataataatt cttattttta
    41161 ttctttttta cctgcctgac ggaatgtcag gttgtagcgg cagtcggtgg tgagtggatg
    41221 aaaccccgct ttcaacggtt gaataccgtg ataaaacagc cgcgattcac cgccccatac
    41281 caccacatcg ccatgttcca acaacaaacg tttgagcgga tcattacgtt tcaggccacc
    41341 aaattgaaaa atcgcgggta agcccagaga aacagaaaca attggcgcgc gcagatccgg
    41401 ttcgtcttta tcctgatgca gcgacagttt cgcgccagga gcgtagcggt tgataagaca
    41461 agcatctggc tggaaatctg gatagcccgc cgccgtagcc gcacgttgac ataaatcatg
    41521 aaagctttgt ggcatagcgg gccacggttt atttgtttgc ggatcaatgg gcgaatagag
    41581 ataaccttgc cgatgggtcg tccagcccag atgcccacag ttggtcatcg ccaccgacat
    41641 ggtatatccg cctggagcga ccatctggcg aaacggcgac tggctggcaa cgtcattaat
    41701 atcgcggatt agttgctccg cagcattaaa agcaaaacgc cgtaaaatta ccgcaccagc
    41761 cgccagtggc tcttgccacg gttcagcatc ggcaaacaga tccaacatta cctctcctca
    41821 ttttcagctt cgcggcgcag cagttgcgct ttacgcgaca cgccccagcg gtaaccggaa
    41881 agtgtgccat caccacggac cacccgatga cagggtatta cgatagccag cttgttggcg
    41941 gcacaggcgc tggcaacggc ccgtaccgct ttcggtttgc cgatggcgtt agccagttgc
    42001 tgataactga cggtttcacc acaaggtatc gtgcgcagtg cctgccagac ttgttgctga
    42061 aaagcagtgc cgcgaatatc cagcggtaac gtcagcggcg tatcgcgttg attgaggctg
    42121 gcgatcactt cacgcacatg ttgctgaaac gtcagatcgg caggcgcgtt gtcggcagcg
    42181 ggaaacatct gctgcaactc gctgattaat gtcgcgtcat catcgcccag caatatcgcg
    42241 caaatccccc gctcgctttc tgccaccagg caacgaccca gctcacaatc agccagcgcg
    42301 taacgcaccg ccagattttc gccaccgtga cggaattgtt tagccgtcat gcccagcgtt
    42361 tcatcggctt tgcggtagta actgctgctg tcggggaatc cggcgttaag aatagacgtc
    42421 gtcacgctct cccctttcgc cagcgattcg cgcaaacggc gagcgcgcca ggcctgttgc
    42481 caggctttag gcgtcattcc ggtagtagct ttaaacaacc gatgtagatg aaatggactc
    42541 atcgccacct ggtcggctaa ggcttccagc gttacaggcg tttcctgttc cagcagtcga
    42601 cacgcgtggg tgattttatc caaccgatgt tgccggggat tggctttgtc tggctgacaa
    42661 cgtttgcagg ggcgaaatcc ggcggcgagt gcctcgctgg catttgcgta gaaggagaca
    42721 ttttcccgca aggcatgtct ggcgcggcaa gaaggacggc aaaagatacc cgtggtgcgc
    42781 acggcgaaaa cgaattcgcc gtcggcattc gggtcgcggg ctaagacaga ttgccagcgt
    42841 tgatcgtcag ttaagcatgt ggcgtttttc ataatcagct ccctggttaa ggatagcctt
    42901 tagtctgccc ggtcaccatc acgcaaaaac caacaatctt gcgctttaat ttttttcgct
    42961 gacaaggaag cttttaaact gtggtgacat ccaggtttta aagctatcgc cttctttggt
    43021 gatcatataa accgccagcc cttcccggcg aacaacctct ttggctttct ccggcccgag
    43081 taccatcaag ccagtatccc aggcatcggc ttccagcgcc gtcggagcaa tcaccgtcac
    43141 ggataccaga ttgtgttcga tgggacgccc ggtttgcgga tcgataacat gggaaagacg
    43201 tttgccgtcc agttcgtaat agttacggta gctgccagag gtgctaatcc catgaccgtt
    43261 gatatccacc acagcctgaa ccgcgttttc tttatcggtt ggtttttgaa tcgctacccg
    43321 ccacggctgg ccttcaccgt tcataccacg gctgttcagc gcgccgccca ccgacaccag
    43381 atagcgggaa atcccttcct gctccatcaa gcgtgccagg tgatccgccg cataaccttc
    43441 accgacggtg gagagatcga catataaatc cggcaggtct ttttgcagat attgctgatg
    43501 cgactgattg atgaccgtca ggtgctgtaa gccggttttg gctttcatcg catcgatctg
    43561 ttcctggctc ggaatttgaa ccggctgttg ttccgggcca aagccccaca gattcaccag
    43621 cggcccgacg gtaatatcca tcgcaccatc ggtccttgcc ccaatgcgca gcgaggtggt
    43681 gacgatatcg gccatcgctt cacttaccgg ccacggcgac aaactttgcg agtcgttaaa
    43741 gcgcatcagc gcggaatctt ttttataggt cgaaagcagc tgatcgtcgg cgtccagctg
    43801 ggtctgaatc ttttctttaa gttcggcact gcgtttggca tcaatgcccg ggatgctggc
    43861 acgccagaag gtacccatgg ttttgccttc aagaacggta acttcagtag cgtgggtttt
    43921 ggcgggttgt ggtttttgat cgcaaccaac aaagaagagc gcggcaacca gcagtgccac
    43981 gcgggtaaag cttatttcca tacgtgatta tcctcatgcg aacggtcgca agagtacacc
    44041 aaaaaactgt gtttgtacgc tgaaaacaat gaaaaaaggg cccgcaggcc ctttgttcga
    44101 tatccatcga gattagaact ggtaaaccag acccagagct acgatgttat cagtgttgat
    44161 gccagcgtca cgagtgaact ggttgtcgtc cagcaggttg attttgtagt caacgtaggt
    44221 ggacatgttt ttgttgaagt agtaggtcgc accaacatca acatatttca ggatatcttc
    44281 gtcgtcgtag ttacgaccat tgatgacacc caggttttta cctttagact gcaggtaagc
    44341 cagggacgga cgcagaccga agtcgaactg gtactgagca acagcttcga agttctgtgc
    44401 tttgttcgcc caacccaagt tgcctacgcg agttgcgttg taggtctggg tgtactgagc
    44461 agctaggtag atgttgttcg cgtcgtattt cagaccaccg gtgtaggttt cagcgcggtc
    44521 gccattaccg atgtaacgct catcataacg gttcagaccg aagttctggt catcagtacg
    44581 tttggagctg gaaactgcag caccgatacc gaagccttcg taatcataag taatagaacc
    44641 gccaacgccg tcaccgttct gacgcagagc ttcacgaccg ttgttggtca tgccttcacc
    44701 ttccgggcta ccgtttttgc cctgatactg aacagcgaag ttcagaccgt caaccagacc
    44761 aaagaagtcg gtgctacggt aggttgcgaa gccgttacca cgctgctgca tgaagttgtc
    44821 agaaccgtaa gtgtcaccac cgaattctgg cagtacgtcg gtccaggaag ttacgtcgta
    44881 aactacgccg tagttacgac cgtagtcgaa agaacctaca tcctggaatt tcagacctgc
    44941 gaatgccaca cgggtccagg agttgttttc gttttcagcg ctgttgccct ggatctgata
    45001 ttcccactgg ccgtaaccgg tcagctggtc agtaacctga gtttcacctt tgaagccaag
    45061 acgcatgtag gtctggtcgc catctacaga cttgtcgtca gagaaatagt gcaggccgtc
    45121 tactttaccg tacagatcta atttgttgcc gtctttgttg taaacttcag cagcgtttgc
    45181 tgcgcctgct accagcagag ctgggaccag gagggacagt actttaactt tcatgttatt
    45241 aaccctctgt tatatgcctt tatttgcttt tttatgccac tgcatactga ttaaccctca
    45301 ttaatcagtc ggcaagtcca ttctccccaa aaatgcagaa taatccaaca cgaatatgat
    45361 actaaaactt ttaagatgtt tcatttatcg ctatagatgt ttcaaaatgt aaatacaagg
    45421 gaacttttta agattattgc ggaatggcga aataagcacc taacatcaag caataataat
    45481 tcaaggttaa aatcaataac ttattcttaa gtatttgaca gcactgaatg tcaaaacaaa
    45541 accttcactc gcaactagaa taactcccgc tatcatcatt aactttattt attaccgtca
    45601 ttcatttctg aatgtctgtt tacccctatt tcaaccggat gcctcgcatt cggttttttt
    45661 acccttcttt acacactttt cattattctg tgctaccaca gaaaaactat aacgcttgtt
    45721 aactatttca caaataatta acatcagcat aatttccagc aatctttgtt tatttgcaat
    45781 taatttcgtt gggctttttg taggttattt gtacagcaaa atggcgcttg tacatctatt
    45841 tccccccata caggatgata aatatcacgg gagaatagag aatcatcaat caggtaagag
    45901 tctggaattt cacactgtac cctttatact gccctatcac ttcgcgaagt tttaacaggt
    45961 cataaacacg aatgcgtcag aaagagacaa cggccacgac ccgcttttca ctcctaccgg
    46021 ggagcattac ccgcttcttt ttactgttga tcattgtgtt actggtgacg atgggggtaa
    46081 tggtacaaag tgccgttaac gcttggctga aagataaaag ttaccagatt gtcgacatta
    46141 cccacgcaat ccaaaagcgc gtcgatacct ggcgttacgt gaccaggcag atctacgaca
    46201 acattgccgc gacgacctcc ccctcctccg gcgaaggttt acaagagacg cgcctgaaac
    46261 aggatgtcta ctatctggag aaaccacgcc gcaaaacgga agcgttaatc tttggctctc
    46321 acgacaactc aacgcttgag atgactcagc ggatgtccac ttatctggat acattgtggg
    46381 gtgcagaaaa tgtaccgtgg tcgatgtatt acctgaatgg tcaggataac agtctggtgc
    46441 tgatctcaac cctacccctt aaagatctca cctccggatt taaagaatcg accgtcagtg
    46501 acattgttga ttcacgtcgt gcagaaatgt tacaacaggc caacgccctc gatgaacgcg
    46561 aaagtttttc taacatgcgc cgcctagcct ggcagaacgg tcattacttt accttgcgta
    46621 ccacgttcaa ccagccggga catctggcaa cggtcgtggc ttttgatctg ccgattaatg
    46681 atttgatccc accgggtatg ccgctggaca gtttccgcct tgagccagac gcgacggcaa
    46741 cgggaaacaa tgataatgag aaagaaggga cggatagcgt cagtatccac tttaacagta
    46801 cgaagattga aatctcctcg gcactcaact ctaccgatat gcgcctggtc tggcaggttc
    46861 cttatggcac cttattgctg gatacgttgc aaaacattct gctgccactg ctgctgaaca
    46921 tcggtttgct ggcgctggcg ttatttggct ataccacatt ccgccatttc tccagccgca
    46981 gtacagaaag cgtccccagc acggcggtca ataacgaatt gcgcatttta cgggcaatca
    47041 atgaagagat agtctcactg ttgccgctcg gcctgctggt tcacgatcag gaatcgaacc
    47101 gcactgtcat aagtaacaaa attgccgatc atttgctgcc gcatctgaat ctgcaaaaca
    47161 tcaccaccat ggcggaacag catcagggga ttattcaggc gacgatcaat aacgagctgt
    47221 atgagatccg catgttccgc agccaggtcg cgccgcgcac acaaattttc attattcgcg
    47281 atcaggatcg cgaagtgctg gtaaacaaga aactcaagca ggcgcagcgt ctgtatgaga
    47341 aaaaccagca ggggcggatg acctttatga aaaacattgg cgatgcgctg aaagaacccg
    47401 cacagtccct ggcggagagt gcggctaaac tcaacgcccc ggaaagcaaa caactggcga
    47461 atcaggcaga tgtgctggtg cggctggtcg aagaaataca gttagcgaac atgcttgcgg
    47521 atgatagctg gaaaagtgag acggtgctgt tctccgtgca ggatttaatt gatgaagttg
    47581 tgccttcagt gttgcctgcc atcaagcgta aaggtctgca actgctgatt aacaatcatc
    47641 tgaaagcaca cgatatgcgc cgcggcgatc gcgatgcctt acgacgtatt ttgctgctac
    47701 tgatgcaata tgccgtgacc tcaacgcaat tgggaaaaat cacccttgag gttgatcagg
    47761 atgagtcctc tgaagaccgc ctgacattcc gcattctgga cacgggagaa ggcgtaagta
    47821 ttcatgaaat ggataatttg cacttcccgt ttatcaacca gacccaaaac gatcgttatg
    47881 gaaaggcgga cccgctggca ttctggctga gcgatcaact ggcacgtaaa ctgggcggtc
    47941 atttaaacat caaaacgcgg gatgggcttg gtacacgcta ctctgtgcat atcaaaatgc
    48001 tcgccgctga cccggaagtt gaagaggaag aagagcgttt actggatgat gtctgcgtaa
    48061 tggtggatgt tacttcggca gaaattcgga atattgtcac tcgcctgtta gaaaattggg
    48121 gtgcaacctg tatcacaccc gatgaaagat taattagtca agattatgat atctttttaa
    48181 cggataatcc gtctaatctt actgcctctg gcttgctttt aagcgatgat gagtctggcg
    48241 tacgagaaat tgggcctggt caattgtgcg tcaacttcaa tatgagcaac gctatgcagg
    48301 aagcggtctt acaattaatt gaagtgcaac tggcacagga agaggtgaca gaatcgcctc
    48361 tgggcggaga tgaaaatgcg caactccatg ccagcggcta ttatgcgctc tttgtagaca
    48421 cagtaccgga tgatgttaag aggctgtata ctgaagcagc aaccagtgac tttgctgcgt
    48481 tagcccaaac ggctcatcgt cttaaaggcg tatttgccat gctaaatctg gtacccggca
    48541 agcagttatg tgaaacgctg gaacatctga ttcgtgagaa ggatgttcca ggaatagaaa
    48601 aatacatcag cgacattgac agttatgtca agagcttgct gtagcaaggt agcctattac
    48661 atgaacaata tgaacgtaat tattgccgat gaccatccga tagtcttgtt cggtattcgc
    48721 aaatcacttg agcaaattga gtgggtgaat gttgtcggcg aatttgaaga ctctacagca
    48781 ctgatcaaca acctgccgaa actggatgcg catgtgttga ttaccgatct ctccatgcct
    48841 ggcgataagt acggcgatgg cattacctta atcaagtaca tcaagcgcca tttcccaagc
    48901 ctgtcgatca ttgttctgac tatgaacaac aacccggcga ttcttagtgc ggtattggat
    48961 ctggatatcg aagggatcgt gctgaaacaa ggtgcaccga ccgatctgcc gaaagctctc
    49021 gccgcgctgc agaaagggaa gaaatttacc ccggaaagcg tttctcgcct gttggaaaaa
    49081 atcagtgctg gtggttacgg tgacaagcgt ctctcgccaa aagagagtga agttctgcgc
    49141 ctgtttgcgg aaggcttcct agtgaccgag atcgctaaaa agctgaaccg cagtattaaa
    49201 accatcagta gccagaagaa atctgcgatg atgaagctgg gcgtcgagaa cgatatcgcc
    49261 ctgctgaatt atctctcttc tgtgacctta agtccggcag ataaagacta atcatctgta
    49321 ggccagataa gacgcgttag tgtcttatct ggcatttgca ccgattgccg gatgcggcgt
    49381 aaacgcctta tccggcctac gattcccatt atttcaacag attacattaa agtaggccag
    49441 ataagacgcg tcagtgtctt atctggcatt tgcactgaat gccggatgcg gcgtaaacgc
    49501 cttatccgtc ctacgaatcc cgcgatttcc tgaccctctc ggcatatacc gtcagcgtct
    49561 gttttatcac atccagcgtt accggcttcg agaggcagct gtccataccg gactccagac
    49621 accgctgctt ctcttcagcc aacgcattag cagttactcc gattaccggc aacgtcagtc
    49681 ccaactgacg aatgcgttgc gtcaagcggt aaccatccat atttggcatg ttgacgtcgc
    49741 taagcacgat atcaatatga ttcttactaa gtacattaag cgcatcgacg ccatcattcg
    49801 cggttttaca ttgatagccc aacgatccca actgatctgc cagcagacgt cggttaatcg
    49861 gatgatcatc cacgaccaga atcatcatat cgtcgttatc gctgaccgct ttgtccgtcg
    49921 acggcagagc gttagcagga tcgtcgctct ccatctcgat caaataaata cgcgccaaca
    49981 atgccggtag ctcatgcgga gcagccacac tgtgtaccca ctcccctggc gctttctcca
    50041 gcggaatacc aatatggcga cgacagaagg tcactaccgc tctgccctgc cattttttac
    50101 tcactacctc gtcagtgatt aacacatctt cgggagtcgg ttcctgccct tcgtatgttg
    50161 taacgacgat gccgctgcgc tgcaaactgg tttccaggaa ctgacagagc gacgcattgc
    50221 ggaccgccag ccagcagcgt ttaccactca acccttccac gccttttttc tgcgggtact
    50281 gagcgccgta caacggaata cgcacggtaa actggctgcc cattcccggt tctgaatcta
    50341 ccgagatatc gccgtccatc atgctgatca gtttttcaca aatcgccaga cccagaccgg
    50401 tcccctggaa gtttcgctgt acgcccgttc caacctggaa gaagggatca aacaagcgca
    50461 ccacttcttt cgccggaatc cccacgccgg tatcgcgaac acggatagag agataatcgc
    50521 catcagcgcg aacatgcaaa actatacagc cggtatcggt gaattttatg gcgttactca
    50581 acaggttgga gatgacctgc tgtaaacgca tcgggtcgcc atttaaggcc actggcacat
    50641 ccggttcaat aaagcagtac aagcctaact gcttgcgtac caccagcggt aaatagttgg
    50701 cggtgatgtg gttcatcact tcacgcggtg aaaactcacg cggttcgatc ttcaactgtt
    50761 ccgattcaat cttcgagaaa tcgagaatat cgctgataat tttcaacaac aggctggaag
    50821 agttgttcat tgccgtcacc agacgatcga cgcctttcgg taactctttg gtttgcaaca
    50881 gatccaggtt accgataatg ccatacagcg gcgttcgcag ctcatgactg acggtggcaa
    50941 ggaacatcga ttttgactgg ctcgcctgtt ccgctgcttg tgccatctcc tgcaacgact
    51001 cttccatctt cacgcgcgaa gaaacatcca ccagcacaca aatggccacg ttttcattac
    51061 gatagcgcga atggacgaag ctgatttgca gattggtatt gttgctggtc aggacatcaa
    51121 caaaattgac ctgctgccca cagataattt gcgtcagtcg ttggcggtcc tcatgcgtaa
    51181 gcatattgag ataggtatgc gccagttcgt tacttaaaat attgacgcca tcagcggtac
    51241 gcaaaatgca gatacccact ggcgcggagg cgacaatctt gcgattgaac tgctcatgtt
    51301 cttccagtcg cagggcgtcg ctttccgccg gaatgaaaat acgtcgctcg tacatccgcg
    51361 cgagagtaaa caatgcagct ccggcaagca cattcagcaa aattgcgtta aggatcaaca
    51421 tgcgaatgcg ttccagcacc ttatcaaccg gcaccgaata cacgatgctt agcgatgagg
    51481 gtggcagatt tttcttcagc accagctccc ggaacccttc cgtatagcca aaccaggagc
    51541 gttcctgcat ccagcgagga tcgcccttaa ttttactttc tggtccggta agcgaaatca
    51601 gggtatgacc attttcatca agaatggtaa cccccatcgg caacgtaccc ggtaagaaaa
    51661 agttctccat ccggatggtc tgctcgacac ccaaaagcgc ctgcaaccgg ttcgccagat
    51721 aaactggcgt caacgcgtaa aaatacccga cgcaagggcg cggaccttcg ctgatccagt
    51781 agaggttact gccgctatca tcttgtggtg catttcgata tttattgatg cgttcatgca
    51841 acgctttcaa cgcggtatcg cgttccactg gcatatcacg cagaccgaaa ttggccatgc
    51901 agaggttatc actgccgatt aaaaataccc ggttgagatc gtaagccgca gaaaaattat
    51961 cgcgccagta gcgcataaac cacgccaatg actccagaga acctcgccag gtgttactca
    52021 ttgcggaaca atcggagtcg gcaaacagcg gttcaaacgc aggcacatcc gcctgcgttt
    52081 ctcgtccacg cggggaaagc acaccgtttt ctgccgataa gcgattttcg gcgatgtact
    52141 taagctcttt catcacatca gaggtgcgtt gaataaagcg ctgagcctga tcggaactca
    52201 gattaaattc ctgacgaatt tccgattctc gctgatgtaa cgcattaacg atgtaaaaaa
    52261 cggatgaaaa agcaatcaac agccagagca ctaacgccaa tgctctgaac atgtagcgcg
    52321 aggctttcag ggttgtacga aaagaagcaa ggtatttcaa aggggagaag ctccgcctca
    52381 ggtgaccgat ggagtgtggt taaggtagcg gtaaaagcgt gttaccgcaa tgttctgtca
    52441 ccattgcaat aatgcgtatc gcttagaaac cattgcctta accaggaaag ccggagtaga
    52501 tggacagttc tcagtacgaa aaataagaaa tgaccaatct gccgacgctt gttaatggtt
    52561 atatcctggc gtcggcagat cgcccagcag tcttatctga aaaaagagag agccaggttg
    52621 tgtgaaggga tggatcacca gtcaaaactt ttcaccagcg tcagctcgcc agcattacgc
    52681 atcggtacaa taaatgtttc ctgtttctca ttgaccgatc cttcatcggt gatcagcgtc
    52741 agttgggcgg tcgtcaattc cgtttcgctg cgcccaccat agtagttgat atacacctga
    52801 tagcgcccgt gaactggtgc tggcatggcg aaaatctcgg gtccgtaccc cgtcgtgaca
    52861 tccatatcca gtgcaccact gtttttcagc acggtgttac cgtaccaggc gtgttcgcca
    52921 tcgggcgtga caacatggag atcgagatcg gtattgtccg tatcccacga gagaaccagc
    52981 cgtaaacgtg cacgaatagt tcctgtaccc ggcgttgagt aaaactgcat tttttgccgg
    53041 ctttgcccat ccgggctgat gacctgcacg ctgttgctgc cttcagtgaa aatataaggg
    53101 cgtgcaaatg aaccatcgga ttcaattctc tgcggcatac tggcaccgtt gaccaccagc
    53161 cggccctgct gaactttacc cgccgcagca taattcttta ttttgccgcg gatctgcgct
    53221 gagatatttt gatcatctgc catgttgacg gacgacgccg ggtaattgat cgattgtgaa
    53281 aaactggcat cttcgccctc tgccggatgc cagccagaga gcggtgcgtc aatctcgact
    53341 tcctgcacac cttcactgtg agcgacaggc gaaagtgcca ccagtaacaa cggaagaaaa
    53401 atctttcgca tagcattagt ccattaataa ttgtcgggtc agcgtttcga tatagttctc
    53461 atcaagcccg gtgggataac catcaaaggc caagtgcaga tactcatggg ttaaatccag
    53521 ccgatcctga agcgtaaaga agttacgaat aaacagccgt ttctgctgcc gatcggtata
    53581 ggggaaacct gagactaaac gacagaccgc aaacacgtct ggttcgttgt accccgtctc
    53641 gccttgtaat atacgccgcc actgcggcat ttttttcgcc agccaggctt tcgctttggg
    53701 taataactgg caggttgagc gtggagctcc ccagcggcta aggctgttgt cgggataagc
    53761 aaacgcgagt atctggtcgt aacgctcccc ctgccccgcc tgggccgtgg cttgccgcca
    53821 ggaaagtgtc ccttcggtag cgcgactgcc atgataatga accggatcgc ctgcatagat
    53881 gagctcctgc gtccaggcgg tcatcgttcg cgcccccgtg gtcgctggcg aagcagaaac
    53941 acgctgcgtg gcgctgctgt cggggatggt caggcaatca ccttcccgat tggcgttttg
    54001 ctgtaaaaaa gtacgtatcg ccacagtcat ggctttcgct gcctcaggtg gcgtactttt
    54061 cgcctcgcga tccagaactc tggcaacgta ctcttcacga tcgagatgcg actgcaattt
    54121 cagtttgccc ttttcgctca gtaaggtcgt ctcaccgtgg ctgacaaacg taatgtggtt
    54181 accattagta aacgtcacac gatagcgccc attaagtacg ctgggtttta ctgccgtcgt
    54241 actcttttcc gctgtgattt tcttcagcgg atagcgggca aacagctcct cctctacaca
    54301 ttgcccactg gcaacctgcg tcggcactgg caaaacccga ttaagcaccg tcgcataacg
    54361 ggttagtacc gttttgctgg tgccactgcc agtaacccac agcggtgtac catcggttaa
    54421 ccagccagca aaaccaccct gacggatttc ctgtttatca tcggcaaacc agctccaggt
    54481 tttcacccgt acccgactac cgagcatgga tgcgacaccg attttcgcct cgtccagcac
    54541 cacatccagt aagacttcct gagctttgtt ttgcgcaggt aacgtcgcta atgaatcaag
    54601 cagcgattta accgttaccg aggtttctgg cttcagcgtt gtaagagacg ctaaccacgc
    54661 aggtgcctga cgctgttgcc agtattgccc ccacacatct gcgccaatat gcaaccgctg
    54721 cggcgcaaaa tagagcccac aggaacgtac cagcgcggta tcgcgcgtta tgctctcccc
    54781 agcctgacaa caataaacct cttccgggga gtttccgcga cactggtagg gctgctccgg
    54841 ttgatgggta tcctccagcc aggcgtaaac atacagtttc cacaaactcc ctaacggcgt
    54901 ggtgagcgag tcaggtaatg ctgagacttt ggtcacgcct gatgacgaca gctgatacag
    54961 ttgatcgtgc tgcgcaccgc gcagcaccag ttgtaacggc gtctcctccg caagcgttgg
    55021 cagagtaacc aacgccaata gccagacgat tctgcgccag ttcacttatt tcacctgcat
    55081 cccggtccat tcactccctg ccgcgacact ttgctgcgca ggtgcatagg aacgcacata
    55141 acgagcagga ggcagaacga attgcccttt ttgcgagaag cgcagcaaat ggcggaaagt
    55201 gaccgttccc gtcagttctt tcaccggcac catataggcc agttcgccca tttcattacg
    55261 cgctttttcc agcaattgcc cctgctgttt cgcggcgttg ggtttattga ctgaaatgcc
    55321 ccatgttgtg cgctcaacgt cagctcccgg cgggagcggt acttccacct gaccatagcg
    55381 cagaactgca tcctgctcgc tggtaagcgt aatttcatcg agatacagcg catcgctgtc
    55441 aatctcattg ctggtcaccg gctgcagagt aaagctcatc tcttcttcac cggggataag
    55501 ccgatacaac tggcgttcaa cggtcaccgg aatgttactt tgttgagccg tttttgccgg
    55561 ttcacgccag cggacctgca catttgcggc gataattcgt caccaaaaga gagaatgtcc
    55621 ggcacaccct aaccaaccca acgccagtct tcgccccctc cagttaactt atgtttagcc
    55681 catgcgcccg caggcgcagg caaaacaact ggaggcattg tcgccatata tttcgccagc
    55741 cagttcatgg ccagcgcgcg ctcaatagtg gattgctcag cggtcaaacc attaaaatag
    55801 cggccgtctg ggtagcgtca ccaccgctat tgagcagcac aaccgtgtgc gccagcggct
    55861 gattgctgtt tgccgcttta tcccacgcat attgctgaac ctgtggcatc accgtggact
    55921 tcaactgcgc ttttttcagc aacgtcatgg ttaagacgtt tgccaccgca tctgccagtg
    55981 gagattccgg tgtatcgagg atcaggctgt cattgatatc gcggatgtct tcctcagaga
    56041 aatcttcatc tttagttccg cgccgggcga tagcttcatc cccccctttc aacaacgttt
    56101 tgcacggcag attcatctcc tgtgcccacg ccagcaccag cgcccgatgc aataacggca
    56161 tattatccgc ctgctcggca taactgtcga gcatatgctg ccagtattcc ggttgttgcg
    56221 ttacgccgag cgcctggctg gcctgccagt cggcgtacca tgcccatgcc gtaaggaagg
    56281 cgtcaccatt gccatcttca ccccaccagg taaagcgcgc tccgggcccc gccagttgca
    56341 tcagccgcag acggttatcc tgaatcatct gacgaatgtc gttagcggcg gcactttgat
    56401 gatcggcaag cgaacgccag gcgagactga gcgggatcag acggctaccg gtgttgatca
    56461 ccccacccca cggttcatcg actaacgcat caagattgtt gcggaaaatc tcctgcggcg
    56521 tttcactact ttgtagccgg atattactcg cctgctcggg caacatcagc gcgttctcgc
    56581 caccgccgag catgacattt ttctgctgtt caacgggcca gctgttgtcc acaaaagaca
    56641 gttttgtgct aatgctgtcc tgcacttgcc cattttgttg cagttctgca cttaacaagc
    56701 cagattgctg aatattctgc gtcagcgaaa tataattcgc ccctttgtgc agcgtcagcg
    56761 tctggcgcat ctcagcgcct gcaaatttag tcaccagcgc caccggttcg ttatcctgct
    56821 gactgaagat aaacagtcct gccgccggtt tgtcgcccac gcgatacacc gttggcatac
    56881 tccacttcat gtagagattt ttttccgaac gcagataagc acgcccctgc ccgaccagcc
    56941 cgtcgccgtt catcccacgc gcggtgatac gccagcgggt taacgaatca ggcatcagga
    57001 acgtgaaata cgctttgcct tgtttatcgg ttgtgagtga cggcatccat gccgcggtat
    57061 ccacctcttc acgccgtgga cgttcaagca tttttactcg ccgctcgctg cggttagtcg
    57121 cgccaggcgc aaccggctcg ctggagagcg cctggtcata gctgataaac gacagactgg
    57181 agctggtacg cacattgtta cgccccagcg gatagaaaaa tttgccgata ttcggcgcga
    57241 tttctggttg cagcgcgtag atcatttcat cgaccacgcc taccgttagc tgcgcagaaa
    57301 caggtttacc tttcagcgac gaggttaa
//
LOCUS       DAJTYM010000014        55473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTYM010000014 DAJTYM010000000
VERSION     DAJTYM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO575_000865"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO575_000865"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6663932.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO575_000866"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO575_000866"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6663933.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO575_000867"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO575_000867"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6663934.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO575_000868"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO575_000868"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6663935.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO575_000869"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO575_000869"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6663936.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO575_000870"
     CDS             7826..9559
                     /locus_tag="OO575_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6663937.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO575_000871"
     CDS             9708..10559
                     /locus_tag="OO575_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6663938.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO575_000872"
     CDS             complement(10546..10887)
                     /locus_tag="OO575_000872"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6663939.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO575_000873"
     CDS             complement(10889..11767)
                     /locus_tag="OO575_000873"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6663940.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO575_000874"
     CDS             complement(11733..14030)
                     /locus_tag="OO575_000874"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6663941.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO575_000875"
     CDS             complement(14081..14401)
                     /locus_tag="OO575_000875"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6663942.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO575_000876"
     CDS             complement(14416..15495)
                     /locus_tag="OO575_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6663943.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO575_000877"
     CDS             15804..16907
                     /locus_tag="OO575_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6663944.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO575_000878"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO575_000878"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6663945.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO575_000879"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO575_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO575_000880"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO575_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO575_000881"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO575_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO575_000882"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO575_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6663946.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO575_000883"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO575_000883"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6663947.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO575_000884"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO575_000884"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6663948.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO575_000885"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO575_000885"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6663949.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO575_000886"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO575_000886"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6663950.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO575_000887"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO575_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6663951.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO575_000888"
     CDS             30120..30728
                     /locus_tag="OO575_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6663952.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO575_000889"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO575_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6663953.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO575_000890"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO575_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6663954.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO575_000891"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO575_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6663955.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO575_000892"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO575_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6663956.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO575_000893"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO575_000893"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6663957.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO575_000894"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO575_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6663958.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO575_000895"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO575_000895"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6663959.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO575_000896"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO575_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6663960.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO575_000897"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO575_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6663961.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO575_000898"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO575_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO575_000899"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO575_000899"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6663962.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO575_000900"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO575_000900"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6663963.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO575_000901"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO575_000901"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6663964.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO575_000902"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO575_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6663965.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO575_000903"
     CDS             complement(44843..45142)
                     /locus_tag="OO575_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6663966.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO575_000904"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO575_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO575_000905"
     CDS             45895..46494
                     /locus_tag="OO575_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6663967.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO575_000906"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO575_000906"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6663968.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO575_000907"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO575_000907"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6663969.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO575_000908"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO575_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6663970.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO575_000909"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO575_000909"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6663971.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO575_000910"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO575_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6663972.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO575_000911"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO575_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6663973.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO575_000912"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO575_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6663974.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO575_000913"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO575_000913"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6663975.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO575_000914"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO575_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6663976.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13510 a        15031 c        14173 g        12759 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttagactggc cccctgaatc
    55441 tccagacaac cagtatcact tatttaagtg ata
//
LOCUS       DAJTYM010000015        53047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTYM010000015 DAJTYM010000000
VERSION     DAJTYM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(2..313)
                     /locus_tag="OO575_000915"
     CDS             complement(2..313)
                     /locus_tag="OO575_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6663977.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            476..572
                     /gene="ffs"
                     /locus_tag="OO575_000916"
     ncRNA           476..572
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO575_000916"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            692..1045
                     /locus_tag="OO575_000917"
     CDS             692..1045
                     /locus_tag="OO575_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6663978.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1087..2637)
                     /gene="pdeB"
                     /locus_tag="OO575_000918"
     CDS             complement(1087..2637)
                     /gene="pdeB"
                     /locus_tag="OO575_000918"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6663979.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2801..3271)
                     /locus_tag="OO575_000919"
     CDS             complement(2801..3271)
                     /locus_tag="OO575_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6663980.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3387..3938)
                     /gene="maa"
                     /locus_tag="OO575_000920"
                     /pseudo
     CDS             complement(3387..3938)
                     /gene="maa"
                     /locus_tag="OO575_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4110..4328)
                     /gene="hha"
                     /locus_tag="OO575_000921"
     CDS             complement(4110..4328)
                     /gene="hha"
                     /locus_tag="OO575_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6663981.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4354..4728)
                     /gene="tomB"
                     /locus_tag="OO575_000922"
     CDS             complement(4354..4728)
                     /gene="tomB"
                     /locus_tag="OO575_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6663982.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5273..8422)
                     /locus_tag="OO575_000923"
     CDS             complement(5273..8422)
                     /locus_tag="OO575_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6663983.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8445..9638)
                     /gene="acrA"
                     /locus_tag="OO575_000924"
     CDS             complement(8445..9638)
                     /gene="acrA"
                     /locus_tag="OO575_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6663984.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9780..10427
                     /gene="acrR"
                     /locus_tag="OO575_000925"
     CDS             9780..10427
                     /gene="acrR"
                     /locus_tag="OO575_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6663985.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10555..13917
                     /gene="mscK"
                     /locus_tag="OO575_000926"
     CDS             10555..13917
                     /gene="mscK"
                     /locus_tag="OO575_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6663986.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14129..14290)
                     /gene="rsmS"
                     /locus_tag="OO575_000927"
     CDS             complement(14129..14290)
                     /gene="rsmS"
                     /locus_tag="OO575_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6663987.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14304..14831)
                     /gene="priC"
                     /locus_tag="OO575_000928"
     CDS             complement(14304..14831)
                     /gene="priC"
                     /locus_tag="OO575_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6663988.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14901..15278
                     /locus_tag="OO575_000929"
     CDS             14901..15278
                     /locus_tag="OO575_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6663989.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15431..15982
                     /gene="apt"
                     /locus_tag="OO575_000930"
     CDS             15431..15982
                     /gene="apt"
                     /locus_tag="OO575_000930"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6663990.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16111..18036
                     /gene="dnaX"
                     /locus_tag="OO575_000931"
     CDS             16111..18036
                     /gene="dnaX"
                     /locus_tag="OO575_000931"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6663991.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17369..17433
                     /gene="dnaX"
                     /locus_tag="OO575_000931"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18089..18418
                     /locus_tag="OO575_000932"
     CDS             18089..18418
                     /locus_tag="OO575_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6663992.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18418..19023
                     /gene="recR"
                     /locus_tag="OO575_000933"
     CDS             18418..19023
                     /gene="recR"
                     /locus_tag="OO575_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6663993.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19133..21007
                     /gene="htpG"
                     /locus_tag="OO575_000934"
     CDS             19133..21007
                     /gene="htpG"
                     /locus_tag="OO575_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6663994.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21188..21832
                     /gene="adk"
                     /locus_tag="OO575_000935"
     CDS             21188..21832
                     /gene="adk"
                     /locus_tag="OO575_000935"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6663995.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21964..22926
                     /gene="hemH"
                     /locus_tag="OO575_000936"
     CDS             21964..22926
                     /gene="hemH"
                     /locus_tag="OO575_000936"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6663996.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22923..23882)
                     /gene="aes"
                     /locus_tag="OO575_000937"
                     /pseudo
     CDS             complement(22923..23882)
                     /gene="aes"
                     /locus_tag="OO575_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24034..25338
                     /gene="gsk"
                     /locus_tag="OO575_000938"
     CDS             24034..25338
                     /gene="gsk"
                     /locus_tag="OO575_000938"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6663997.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25471..27147)
                     /locus_tag="OO575_000939"
     CDS             complement(25471..27147)
                     /locus_tag="OO575_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6663998.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27385..28605)
                     /gene="fsr"
                     /locus_tag="OO575_000940"
     CDS             complement(27385..28605)
                     /gene="fsr"
                     /locus_tag="OO575_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6663999.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28823..30475
                     /gene="ushA"
                     /locus_tag="OO575_000941"
     CDS             28823..30475
                     /gene="ushA"
                     /locus_tag="OO575_000941"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6664000.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30512..30991)
                     /gene="ybaK"
                     /locus_tag="OO575_000942"
     CDS             complement(30512..30991)
                     /gene="ybaK"
                     /locus_tag="OO575_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6664001.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31195..31989)
                     /locus_tag="OO575_000943"
     CDS             complement(31195..31989)
                     /locus_tag="OO575_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6664002.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32127..32468
                     /locus_tag="OO575_000944"
     CDS             32127..32468
                     /locus_tag="OO575_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6664003.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32683..35187)
                     /gene="copA"
                     /locus_tag="OO575_000945"
     CDS             complement(32683..35187)
                     /gene="copA"
                     /locus_tag="OO575_000945"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6664004.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35449..36381
                     /gene="glsA"
                     /locus_tag="OO575_000946"
     CDS             35449..36381
                     /gene="glsA"
                     /locus_tag="OO575_000946"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6664005.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36384..37676
                     /locus_tag="OO575_000947"
     CDS             36384..37676
                     /locus_tag="OO575_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6664006.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37801..38208
                     /gene="cueR"
                     /locus_tag="OO575_000948"
     CDS             37801..38208
                     /gene="cueR"
                     /locus_tag="OO575_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6664007.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38209..38667)
                     /locus_tag="OO575_000949"
     CDS             complement(38209..38667)
                     /locus_tag="OO575_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6664008.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38664..39581)
                     /locus_tag="OO575_000950"
     CDS             complement(38664..39581)
                     /locus_tag="OO575_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6664009.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39727..40404
                     /gene="fetA"
                     /locus_tag="OO575_000951"
     CDS             39727..40404
                     /gene="fetA"
                     /locus_tag="OO575_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6664010.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40391..41170
                     /gene="fetB"
                     /locus_tag="OO575_000952"
     CDS             40391..41170
                     /gene="fetB"
                     /locus_tag="OO575_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6664011.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41233..42087)
                     /gene="cnoX"
                     /locus_tag="OO575_000953"
     CDS             complement(41233..42087)
                     /gene="cnoX"
                     /locus_tag="OO575_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6664012.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42148..42957)
                     /gene="ybbO"
                     /locus_tag="OO575_000954"
     CDS             complement(42148..42957)
                     /gene="ybbO"
                     /locus_tag="OO575_000954"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6664013.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42947..43569)
                     /gene="tesA"
                     /locus_tag="OO575_000955"
                     /pseudo
     CDS             complement(42947..43569)
                     /gene="tesA"
                     /locus_tag="OO575_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43540..44226
                     /locus_tag="OO575_000956"
     CDS             43540..44226
                     /locus_tag="OO575_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6664014.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44223..46638
                     /locus_tag="OO575_000957"
                     /pseudo
     CDS             44223..46638
                     /locus_tag="OO575_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47351..48445)
                     /gene="mnmH"
                     /locus_tag="OO575_000958"
     CDS             complement(47351..48445)
                     /gene="mnmH"
                     /locus_tag="OO575_000958"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6664015.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48514..49440)
                     /gene="allS"
                     /locus_tag="OO575_000959"
     CDS             complement(48514..49440)
                     /gene="allS"
                     /locus_tag="OO575_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6664016.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49670..50152
                     /gene="allA"
                     /locus_tag="OO575_000960"
     CDS             49670..50152
                     /gene="allA"
                     /locus_tag="OO575_000960"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6664017.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50230..51045
                     /gene="allR"
                     /locus_tag="OO575_000961"
     CDS             50230..51045
                     /gene="allR"
                     /locus_tag="OO575_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6664018.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51135..52916
                     /gene="gcl"
                     /locus_tag="OO575_000962"
     CDS             51135..52916
                     /gene="gcl"
                     /locus_tag="OO575_000962"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6664019.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52929..>53047
                     /locus_tag="OO575_000963"
     CDS             52929..>53047
                     /locus_tag="OO575_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6664020.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYD"
BASE COUNT        12786 a        13656 c        13962 g        12643 t
ORIGIN      
        1 gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat accatcacac
       61 cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc gaaattgtgc
      121 catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga cgctttaaca
      181 caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca tcaccgtaag
      241 tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg ggaaatgaat
      301 cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga aaaacgcccg
      361 agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc aattagccgg
      421 ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg ggctctgttg
      481 gttctcccgc aatgctactc tgtttaccag gtcaggtccg gaaggaagca gccaaggcag
      541 atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc tcccaccgtt
      601 tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca agaattgtct
      661 ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt ttgtggttgc
      721 cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg cgccattgtt
      781 taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtgc cggatggcaa
      841 agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg tctttagctg
      901 gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga tgtcggaccc
      961 acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga tctatggcgg
     1021 attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg ccgtcagccc
     1081 aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta atgccttgct
     1141 gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt cttgtttact
     1201 ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt cgatgatatg
     1261 cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct tcagaatgtc
     1321 gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat catcaagata
     1381 aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt tcggatcggc
     1441 aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat agtgattgat
     1501 catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt caagattgat
     1561 cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa agacgcttct
     1621 tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg gaataaaact
     1681 atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct cagcaccgac
     1741 aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat cacgattttc
     1801 gatagcatcc tgcagccgat gatgcggtga ttggatacgg cgcaaaatac gcagcacaaa
     1861 catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga cttgccgatg
     1921 ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat tcatctccag
     1981 gaagggcagt atatcgtaaa tgatcccatt attttcgata tgctcaccgg gtgttttttg
     2041 tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa cgttatgggc
     2101 attgccaata atggcggcat caatttgcca tgaactatag ggaatgacat caataaagga
     2161 agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt aacggataat
     2221 gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa ttttacctag
     2281 ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg gaacgttgtt
     2341 atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac gcatttccat
     2401 gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca gcgcatcttt
     2461 cccttgtgtc gccaccttat tggctcgaat agcgacgcgg gaggaatagg tatccagctc
     2521 ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc cgacaggcag
     2581 caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg ttctcaccag
     2641 catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa ggctggcagg
     2701 agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc attgtcattt
     2761 gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt gattcggcgc
     2821 gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc attttttgca
     2881 gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca accagcgcgg
     2941 caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa tccagcacgt
     3001 cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca ttaagcagga
     3061 taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc aacatcaccg
     3121 ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt ttacggataa
     3181 aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga gactcaatcg
     3241 tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc tgcaactttc
     3301 cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat ctaccacaat
     3361 tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc tggattaccg
     3421 cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc taccacgacg
     3481 ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca gacgttatta
     3541 ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg gtcgatggga
     3601 tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc gatgcgaata
     3661 gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc gagaaaaatg
     3721 ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac ctgaccgaat
     3781 aaattagcga gaatttactg gcgtaatgtg tgctcttccg ccagggaatg attgtatcgg
     3841 tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc cgagcgatac
     3901 aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg gttgctgaaa
     3961 atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta catctgtgtg
     4021 ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt aataaacagc
     4081 cggttatagc accgaaagcg aattatttat tagcgaataa atttccatac tgaggaaggg
     4141 atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc ggctgagtaa
     4201 aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac acgctccagc
     4261 gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt taaaggtttt
     4321 tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg caggattctc
     4381 tttcgtcgcg ttgacaaaac aacggaatag tcgattacct gacttccgcc atttctgaag
     4441 agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca gatattcgtc
     4501 gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa gtgcgaaggt
     4561 cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg ggtcgttaac
     4621 ccaaccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg tttcacagag
     4681 aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa cgcgtcccct
     4741 tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg acctagactt
     4801 gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat cgcattatta
     4861 cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac tagcactaac
     4921 ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat tatcgccaga
     4981 tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac cgaggaatga
     5041 ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat tattaaatct
     5101 attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat tttatacgtg
     5161 gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc ttaatgttcg
     5221 taggttatgc ataaaaaggc cgcttacgcg gccttagtga ttacatgttg tatcaatgat
     5281 gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg cggcgaacca
     5341 ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc atcccgccca
     5401 ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg ataaccagcg
     5461 gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa cgcatccgca
     5521 ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag tctttggcga
     5581 attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc agtcctactt
     5641 ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca ccgataaccc
     5701 ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac agcgccgcca
     5761 gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg ttgccggaga
     5821 gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc agtttgctcg
     5881 ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc gcctggccta
     5941 agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg tactcccaac
     6001 gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga acataccagt
     6061 cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa actttcttca
     6121 cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg cccagagtgg
     6181 tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg atatcaatct
     6241 taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata tcagggtgct
     6301 tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga ccaaggccag
     6361 cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg attgcgggca
     6421 ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt gcacgcatgg
     6481 taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc aaggaaacga
     6541 acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg gcgaacaccg
     6601 actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg agcactttct
     6661 gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat acgccctggt
     6721 cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg ccgaccacga
     6781 tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct acgctgtcgg
     6841 tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag ccttttttac
     6901 cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga gccggagtca
     6961 ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata gagaactgac
     7021 gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc gccgacagta
     7081 ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg gtagcttctt
     7141 ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca acgatagcgt
     7201 catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg tttatcgaga
     7261 agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg gcaatggtcg
     7321 gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac acgaggatga
     7381 tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac ggcgtggtgt
     7441 cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc gccagttcag
     7501 cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg atccccagac
     7561 cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca cccagctcaa
     7621 tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc agcaggattt
     7681 tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg ttaagctgtt
     7741 ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg ttctgcgctt
     7801 tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca ttcgggttca
     7861 tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg cccgacgtac
     7921 ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc tgcgtcatgg
     7981 tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat ttctcaacgc
     8041 tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc agtttgttct
     8101 gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg gtgatctgca
     8161 cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata ccgttcatat
     8221 tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg ccggggtagg
     8281 aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc ggcagtttga
     8341 ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca aaaatcgggc
     8401 gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga cttggactgt
     8461 tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc ttgtgctttt
     8521 acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc gcctgctttc
     8581 agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat cggacgggtt
     8641 tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg cgtacgggtt
     8701 acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag acgtgcgcgc
     8761 acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg tagggtgata
     8821 gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc ctgcgggaac
     8881 ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg tttcagcgtg
     8941 ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt cacatcaacg
     9001 tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt ctgtaccaat
     9061 gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt gactttggtg
     9121 taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac cgcagcattc
     9181 gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta ctgagtaccg
     9241 agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc ctgggctttc
     9301 gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc aatctgatag
     9361 agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag gataatcccg
     9421 ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg aagctcggtt
     9481 gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat ctgctggcca
     9541 ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga gagcatcaga
     9601 acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg agtgtccgat
     9661 ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata ttatcgtcgt
     9721 gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt cacgaacata
     9781 tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc gatgtggctc
     9841 tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt gcaaaagcag
     9901 ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat ttgttcagtg
     9961 agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat caggcaaaat
    10021 tccctggcga tccactctca gtattaagag agatattaat tcatgttctt gaatccacgg
    10081 tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc gaatttgtcg
    10141 gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt tatgaccgta
    10201 tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat ttaatgacgc
    10261 gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac tggctctttg
    10321 ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc ttactggaga
    10381 tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc tgaatctgac
    10441 tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg tcgtgatatt
    10501 cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt catcatgact
    10561 atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat tgcttttgtt
    10621 tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg tgatctgccg
    10681 acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga tctttctgct
    10741 caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga taaaatcgat
    10801 cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc ggaaaaaatg
    10861 cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga agaaacgcgc
    10921 aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca ggcgctggac
    10981 gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt ttcgttacag
    11041 acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct gcaacaaatt
    11101 cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag ccagaaagtg
    11161 ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg taaaagcctg
    11221 gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt gacggcgaac
    11281 agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag caagcgcctg
    11341 actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc gcgtattcag
    11401 gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca gcgtctgatt
    11461 accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa aaactggctg
    11521 gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct gaagggcagc
    11581 ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc ggatgaactg
    11641 gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt taaccagcag
    11701 cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg tcacaccaac
    11761 gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg tcgcgaattg
    11821 ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa cctgcaaatc
    11881 aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac tcagcaaatc
    11941 ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt cccgcaaagc
    12001 ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc ctggcctgcc
    12061 gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct gatccactgg
    12121 cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg ttccctgcgt
    12181 aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg cgcgctgccg
    12241 gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct caacatcagc
    12301 gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt tggcctgtgc
    12361 tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc ggagcagcag
    12421 accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc tatccatttc
    12481 tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct ggggcaggcg
    12541 atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg ccgcgaaagc
    12601 tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct gtcgattatc
    12661 ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct gcgcctctcc
    12721 gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta ccagacggta
    12781 ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct ggcgcgtcgg
    12841 cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc caccattgca
    12901 ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt tgcgctgttc
    12961 ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta tctcgacagc
    13021 atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa aaacgtcacc
    13081 atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt gattcgcaac
    13141 ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca gggcgcgtcg
    13201 tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat gacggtgttc
    13261 ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt atccgtaggt
    13321 cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat cattctgttc
    13381 gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg gacggtaagt
    13441 aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt gatcatcccg
    13501 aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac tactacgcgt
    13561 ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg taaagtgtta
    13621 ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga agtcttcttt
    13681 acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg tgaactgcgt
    13741 gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg ccgtgaaaac
    13801 gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa gggcgatgag
    13861 gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt tgggtaataa
    13921 ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt tgttcatgcc
    13981 ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat atattgcagg
    14041 agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc cgcaatctta
    14101 ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg tgcgttaatt
    14161 tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg gcagggatct
    14221 gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca ggggcatttt
    14281 caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt ttcgattttt
    14341 tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc acgatgcagc
    14401 gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc ggcaaccatc
    14461 tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca gcgggaaatt
    14521 ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg cgcgatggct
    14581 tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg ttgctgctca
    14641 actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga ctgtagtgtt
    14701 gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc gaactgtgac
    14761 accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc cagcagcagg
    14821 gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag tagccactat
    14881 gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc tggctggcgg
    14941 tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg tttatcctgc
    15001 tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg ctgtaccgct
    15061 catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg cgcggcgtca
    15121 aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg ttcgtccaga
    15181 tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc tatatgtggc
    15241 gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc gcagttgcaa
    15301 ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc ggtcaaaggt
    15361 taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg taacaagcag
    15421 gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata gcatcaaaag
    15481 cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct tactggaaga
    15541 cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa atgcgggcat
    15601 taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg tagctctggg
    15661 tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg aaaccatcag
    15721 tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg atgccatcaa
    15781 accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca ctatcgaagc
    15841 gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt tcattatcaa
    15901 cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca gctacagcct
    15961 tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct acggacagca
    16021 caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac cccttcgtga
    16081 atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc ccgaaaatgg
    16141 cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc actggcgaac
    16201 ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg tggcgtcgga
    16261 aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg cattaccgcg
    16321 acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt tgtcgatctg
    16381 attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct gctggataac
    16441 gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga agtgcatatg
    16501 ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc ggagcacgtt
    16561 aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt gtcacgctgt
    16621 ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct tgagcacatc
    16681 ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc gcgcgccgct
    16741 gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag cggtgacggc
    16801 caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga tcaggcgctg
    16861 tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact gattaatgaa
    16921 gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg cttgttgcat
    16981 cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc cgccatcgag
    17041 ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct ttactatcag
    17101 acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat gggcgttgag
    17161 atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga gccagaagtg
    17221 ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac cctggtgccg
    17281 ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac cagccaggtg
    17341 ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa aaagagtgaa
    17401 ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag actggcttcg
    17461 gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc gccagccaaa
    17521 aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga agtggtcgcc
    17581 acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact ggcggtgaag
    17641 ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca actttcgcta
    17701 ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga caacgcagta
    17761 tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca gcaaaaactg
    17821 gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt tgaagatgat
    17881 aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga aaaacttgcg
    17941 caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg gtttttcgat
    18001 gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct tacgttcgtc
    18061 atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg gtaacctgat
    18121 gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga tcgcgcagct
    18181 ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg gtgcacacaa
    18241 ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga tgctggaaga
    18301 cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc agaaagaaaa
    18361 aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc cgttctgatg
    18421 caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc gggcgttggc
    18481 ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag cggcgggatg
    18541 cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc cgattgccgc
    18601 actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca ggaaaacggt
    18661 caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac ggggcagttt
    18721 tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat cggtccggat
    18781 gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac tgaagtgatc
    18841 ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc cgagctttgc
    18901 gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg cggcgaactg
    18961 gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa gattcgtttt
    19021 taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct tgtccccatc
    19081 tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct acatgaaagg
    19141 acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga tgatccattc
    19201 tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct ccgatgcggc
    19261 ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg atggcgaact
    19321 gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg ataacggcgt
    19381 ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat ccggtaccaa
    19441 atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga tcggtcagtt
    19501 tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc gtactcgcgc
    19561 ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag gtgaatatac
    19621 cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc tgcgtgaagg
    19681 cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat actccgacca
    19741 tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa ccgttatctc
    19801 ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg aaatcaccga
    19861 tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc cgctgacctg
    19921 gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca tcccgtccca
    19981 ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt acgttcaacg
    20041 cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct tcgtgcgtgg
    20101 tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc aggacagcac
    20161 ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc tggaaaaact
    20221 ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc tggtactgaa
    20281 agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc tgcgttttgc
    20341 ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg tttcccgcat
    20401 gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg cagcgaagag
    20461 cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc tttccgaccg
    20521 catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt tccagtcggt
    20581 gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga gcgcgaaaga
    20641 agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg gcgagcgcgt
    20701 gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt cgaccgacgc
    20761 ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga aagtgccaga
    20821 agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg cggcagatac
    20881 tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg cgctgctggc
    20941 agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc agctgctggt
    21001 ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt catcatctgt
    21061 actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt cttgaggcaa
    21121 tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt ttaaggggat
    21181 tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga ctcaggctca
    21241 gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc tgcgtgctgc
    21301 ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg ctggcaaact
    21361 ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg aagactgccg
    21421 taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg cgatgaaaga
    21481 agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac tgattgttga
    21541 tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg ttaaattcaa
    21601 tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta cccgtaaaga
    21661 cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga ctgcaccgct
    21721 gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga aagttgacgg
    21781 taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct aatcaagccg
    21841 cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct ctcacagcaa
    21901 ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg ataagaggcg
    21961 gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc cgatgccccc
    22021 acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg cgtggttgat
    22081 acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct gcgctcgccg
    22141 cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct gatggtttac
    22201 agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt ggcgctgggc
    22261 atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc agagcatgta
    22321 gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt cggtgcggta
    22381 tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat atcgtttatt
    22441 cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt acgcgcttct
    22501 tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat tccccagcgt
    22561 tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg cgaactggct
    22621 tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg ctttggccgg
    22681 gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga aaaaggcgta
    22741 ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac gctggaagag
    22801 attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata tgaatatatt
    22861 ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt tgccgcgtat
    22921 cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc ggcggttttc
    22981 atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt gaactcacag
    23041 ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag cagcggatcg
    23101 aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt attaaacaga
    23161 cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc atacatctgc
    23221 aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt cacggaatcc
    23281 cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca atcgatctgt
    23341 ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga atcaccggca
    23401 aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa ataacaacaa
    23461 gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag ggtgtaatta
    23521 ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg atcgtgggta
    23581 tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc cgggctatct
    23641 ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac catgtaagct
    23701 ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt gtaatactgt
    23761 cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg aagagtattc
    23821 acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt ttccggcttc
    23881 atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga aaatgcgatc
    23941 ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt gtgttaacat
    24001 tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa acgtaaatcc
    24061 aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca gccagaaaac
    24121 gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat tgaagcgaaa
    24181 gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact ggtgattgag
    24241 gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat tacccatcag
    24301 tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc ggacgaccgt
    24361 tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc ctatcgttac
    24421 ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga tggtccgatt
    24481 ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat cagtccaggc
    24541 cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg agcctcggca
    24601 ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc ggaagcaacc
    24661 atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac gctgggcacc
    24721 aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga ccacgtttct
    24781 atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga tccgttgttg
    24841 gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg gccaatcggc
    24901 ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca tccgctgctg
    24961 ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg ccacaaggat
    25021 tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg gccggaaaaa
    25081 atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca tgacattacc
    25141 gccaacagct accatcgtag caacgtacca aactccagca aacataaatt cacctggtta
    25201 acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta tcaggtactg
    25261 aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag ccaggaagag
    25321 tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt tcacgccgca
    25381 tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat gcctacatat
    25441 ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac ccgccggtgg
    25501 cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca ctacctgatt
    25561 cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg cgataatctc
    25621 aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat aaccgttggg
    25681 gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa tttcttcgtt
    25741 cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa cacgggttcg
    25801 tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc ccagcaggct
    25861 gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg ggatctgttt
    25921 ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct tcgccagata
    25981 tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg cccctgccag
    26041 taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc ccgccaggat
    26101 aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg tacgttggga
    26161 gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca gaataattgc
    26221 cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa acaacatccc
    26281 gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat cgtgggcggc
    26341 acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac cgagtgcaaa
    26401 ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg ccagcactga
    26461 cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc acggcaccag
    26521 acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga tggtgatccc
    26581 catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc ctgccactgc
    26641 gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc aaccgatggc
    26701 gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca gtaacaccac
    26761 ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg accagcccag
    26821 cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga tcgcgccggg
    26881 aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac cgacgccgaa
    26941 catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat cggcaacaaa
    27001 gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca gaggagaaat
    27061 acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc caacaatggt
    27121 ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt attgtccatt
    27181 tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt gatgatgttt
    27241 atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag cacggttcgg
    27301 aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg atgcagccaa
    27361 agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca ggaatatggt
    27421 caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga tgctggtgtg
    27481 atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag caaaaccgaa
    27541 aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt agaccagaat
    27601 ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccgtccagtg
    27661 caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat
    27721 cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac ctgccgcaac
    27781 ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc cgaatttttg
    27841 catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt tcgagaaaat
    27901 gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg gattgattat
    27961 cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac ggctaatttg
    28021 cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc
    28081 ataaggctcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa agttgccgcc
    28141 gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac gggctacgcg
    28201 agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg ccagcagaac
    28261 tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa agcacatgcc
    28321 aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca ctggttgcag
    28381 tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa atgtcagaga
    28441 aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca tgtcgttcag
    28501 cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct tggtcgttga
    28561 cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa agtcactttt
    28621 tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta acatacctgt
    28681 atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg gatgcagatt
    28741 tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt caacacgttg
    28801 aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt
    28861 aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata aaacctacaa
    28921 aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg aatatggcga
    28981 atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg ttgcggctga
    29041 aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc ccgagtctga
    29101 cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg acgcgatggc
    29161 gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg aaaagtgggc
    29221 caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc gcctgtttaa
    29281 accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc tgacaaccga
    29341 tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc gtaagcccgc
    29401 cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag acattattat
    29461 cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg caccgggcga
    29521 tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc
    29581 gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg tgccgggtac
    29641 gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg agtggggcaa
    29701 atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg ttaactacca
    29761 gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt
    29821 gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat caccgttcca
    29881 gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc gtctggaagg
    29941 cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc tggcagccca
    30001 aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc gtgattctat
    30061 cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg gcaatgtggt
    30121 ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg tcgcgcagat
    30181 gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg
    30241 caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat
    30301 ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg ataacaaacc
    30361 gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata tccagaaaag
    30421 ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc agtaatccga
    30481 aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt
    30541 tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt ccgcgcttgc
    30601 cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc gttggcagac
    30661 gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa cgctgcgcga
    30721 ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact tttttaagat
    30781 ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca ccgttcactg
    30841 ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg acgacttcat
    30901 cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt
    30961 ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac
    31021 gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct aagagtattg
    31081 gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg acggcataat
    31141 aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat ttttttagcg
    31201 caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg cgaccacata
    31261 tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct gatgcatcag
    31321 cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat tttgcggcgg
    31381 tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat gccagtgggt
    31441 cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca tggcaatctg
    31501 gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg cctgcaatag
    31561 ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag
    31621 aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa tgcccatctc
    31681 ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt ccagcgcctc
    31741 gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct cgacgatcag
    31801 cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca tatcgtggct
    31861 acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg tgatatcgat
    31921 ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta cccggtacaa
    31981 cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca
    32041 aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt gcatcgctat
    32101 tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg acgacacccg
    32161 gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat gagttagcag
    32221 agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat cgtaaactca
    32281 ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat tttaggctaa
    32341 acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc caggaagaat
    32401 tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg
    32461 tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc acgccgcatc
    32521 cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc ctacaaacct
    32581 gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg
    32641 ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct tcggtttaaa
    32701 ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca ttgccgctcc
    32761 ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg cggcgaccgg
    32821 aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt tgcgcagcgt
    32881 tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca tcagggtaat
    32941 cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga catccgcctg
    33001 ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac gtccttcact
    33061 ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa tcacttcatc
    33121 aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg tcaacatcac
    33181 cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg
    33241 atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca caggcgttgc
    33301 tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga cttgctgatc
    33361 atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac cgctcacgcc
    33421 cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc ctgctttatc
    33481 aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg ccagacgcag
    33541 cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg gcttcccttc
    33601 ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc gttgcagcgc
    33661 gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg aaataatcga
    33721 catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg tagtggcaat
    33781 caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg ccgcactgac
    33841 aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg ccagctgacc
    33901 gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg atagcgtggt
    33961 atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca ctgtcccggc
    34021 atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca gcatcgcttc
    34081 atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat cgccggtcgt
    34141 caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt caccttcgtc
    34201 agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg ccttagaaga
    34261 acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga taatcatcgc
    34321 actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc acaggttgac
    34381 gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat ccatcgtcgc
    34441 tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg cgaaaaccat
    34501 caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg cggtgactat
    34561 catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca gtgcgacaat
    34621 tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc gttcgcggcg
    34681 tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg cctgcactaa
    34741 atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc
    34801 ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac agctggcgca
    34861 gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg ctgccggaag
    34921 cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca gcggtttagc
    34981 ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga tcagctgttc
    35041 tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt gttccacatc
    35101 cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc
    35161 caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt tgactggctg
    35221 tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac caggcgggta
    35281 aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca tttatttctt
    35341 tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga aaatcggcac
    35401 tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat gttagatgca
    35461 aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact taacggcgga
    35521 caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc ggcagtggct
    35581 atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg ctttgcactg
    35641 gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg
    35701 gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt tatcgcctta
    35761 gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat tgccaccacc
    35821 agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca tatccaacag
    35881 caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga acaaacaacc
    35941 aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct ctattgtgat
    36001 gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa tactgttgaa
    36061 ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac gcataaacgc
    36121 gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga agggctgtat
    36181 ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc
    36241 ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc accgctggac
    36301 gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc
    36361 tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt aacaaacccc
    36421 tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg gggatcttcg
    36481 cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc tttgcttttg
    36541 gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg agctatccca
    36601 gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc ttttcgctag
    36661 cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt gcccgtgctt
    36721 ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac cttattttgc
    36781 tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg
    36841 tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta
    36901 ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc cccagctccg
    36961 gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt ggcatgatgg
    37021 cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg tttctggtgg
    37081 cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt agcgatgtat
    37141 cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct tctccgctgc
    37201 tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg gcttcaggca
    37261 ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc gaacgcgaac
    37321 tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat atcatcgtcg
    37381 tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc agcgttgcca
    37441 gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg
    37501 atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg
    37561 ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg attattggtg
    37621 caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc gtataacacc
    37681 tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc actggcaaaa
    37741 aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg
    37801 atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat tcgcttctat
    37861 gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg cacctacacg
    37921 cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg ctttaacctg
    37981 gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag cgccgacgtc
    38041 aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga gctgcaatcc
    38101 atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag cgccgactgc
    38161 ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt aagacgagac
    38221 ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg tacctgcacc
    38281 gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc
    38341 gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt
    38401 taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag
    38461 ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc aacccagcgg
    38521 caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc
    38581 atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc agaaaatatg
    38641 tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct gtctttcacc
    38701 agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact
    38761 actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt ttgcgctacg
    38821 aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat tttggtggcg
    38881 cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt
    38941 tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc
    39001 tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc
    39061 gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg
    39121 actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg tgagttgatg
    39181 ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt acggatgtta
    39241 gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc agcgcgtggc
    39301 gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag
    39361 ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg
    39421 cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca
    39481 aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg
    39541 accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt
    39601 accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag
    39661 agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga
    39721 acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt
    39781 gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt
    39841 accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc
    39901 ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa accagaaatc
    39961 taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga tacggtatac
    40021 gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc catttttctc
    40081 gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat cgccgagcta
    40141 tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat gccgaaggtt
    40201 ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa cgtcaatgag
    40261 atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac acacgataaa
    40321 gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg agaaatgcag
    40381 gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg cattaatgct
    40441 ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct
    40501 atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc tgaagtatat
    40561 tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc
    40621 ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat
    40681 tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag gggcgattga
    40741 atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg ccatggtagc
    40801 ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca
    40861 ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag
    40921 tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt
    40981 accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata
    41041 tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca ttattgcctg
    41101 ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga ctcaattgaa
    41161 gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg
    41221 aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc
    41281 atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc
    41341 ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc
    41401 aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg gattctccgc
    41461 cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca gcagttcgat
    41521 ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac
    41581 cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca gcaggccgat
    41641 ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg catcggtgta
    41701 attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag
    41761 cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccatctac
    41821 cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa actgggcggc
    41881 aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt tgtactgcgc
    41941 cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt cagaccaaaa
    42001 atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt
    42061 aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga
    42121 catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc
    42181 aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg gatagcgcat
    42241 cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt ccggtcccaa
    42301 cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac
    42361 gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct
    42421 gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt tactggccgc
    42481 gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca tcacaatacg
    42541 cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa
    42601 aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa gggggccata
    42661 catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgatcac
    42721 ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca cgccggtaaa
    42781 tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca gcacatgaaa
    42841 accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac atccggtaat
    42901 taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat gagtcatgat
    42961 ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt
    43021 tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct tccataaaaa
    43081 agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt
    43141 cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt
    43201 tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa
    43261 aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct gatgctgttt
    43321 gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga tgctggcatt
    43381 aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg ccgcgctggc
    43441 agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt ccgctgcggc
    43501 ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga aaacattgtt
    43561 gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc catcctcacc
    43621 ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga gtcgggatcg
    43681 ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag tggcgaagtg
    43741 agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa gttgcgcgcg
    43801 aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa cgcgctggaa
    43861 aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg taacggggcg
    43921 aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc ggcacagctt
    43981 tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg acctgatgtg
    44041 ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa aattgccgac
    44101 ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac ccacgacctg
    44161 caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt gcaggaggaa
    44221 gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta attgtctggc
    44281 tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc gatcgcatgg
    44341 agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg ttgcgcagtt
    44401 cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg aaagtcggca
    44461 agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg gcgaacgtca
    44521 aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc cctggcctga
    44581 aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt aacctgaaaa
    44641 cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa gtgattcagg
    44701 aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg aatctggcgg
    44761 atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt tataaattcg
    44821 gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag cttaaacccg
    44881 aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg atggaacgct
    44941 cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg gcagcggtgg
    45001 cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc ctcaaaacgc
    45061 tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg atggtgctga
    45121 cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg ttgatggtgc
    45181 tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg tggctgtggg
    45241 cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac cgcttgttgc
    45301 tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc tggccgctga
    45361 agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga ttaatgggtg
    45421 gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg ctgtgcggtg
    45481 tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg ctgcctctgc
    45541 gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag ctttcggcat
    45601 tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat ctgctcgacc
    45661 gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac atcgccacag
    45721 aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg gaatcatttt
    45781 atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa ggtaatgaag
    45841 atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc gatcataatc
    45901 cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa gaggggctgg
    45961 cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat acccaggagt
    46021 tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg cctaatttct
    46081 attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt accagtttcc
    46141 gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg accattagcc
    46201 tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag gtaagtcggg
    46261 cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg ctggcacagg
    46321 tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca ctcggtgcgg
    46381 ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg gtttgtttcc
    46441 ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc gaaagtgttt
    46501 gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag cggtgcgctg
    46561 ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa ggcgctgttc
    46621 aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc ttatcaggcc
    46681 tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata aggcgtttac
    46741 gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa ctgtcatgtc
    46801 ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc ctcagttatt
    46861 caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg atgagaactt
    46921 tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca cagcttaccc
    46981 ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc tctctccaaa
    47041 ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt acttttccgc
    47101 actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc gccaacatag
    47161 ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg actgtttaat
    47221 ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact gtcatgtcgg
    47281 tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg ccgtttcaga
    47341 ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca ccgcgaaaga
    47401 caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa tattctttaa
    47461 gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt tgcgttgtca
    47521 gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc cgacgcttaa
    47581 tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt tcgtcgccat
    47641 aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg cgttcaagac
    47701 ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc tcgcgcagac
    47761 attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc cacaggcgca
    47821 aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca aaactcgcct
    47881 ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc gccagccctt
    47941 ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg ccggtacaac
    48001 cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc gcggtctggc
    48061 gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca tgcagccagc
    48121 gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct tgcggatttt
    48181 gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc gccaccagtt
    48241 tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg ccaacggagg
    48301 cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg ccttgctcaa
    48361 actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg gcacgatagt
    48421 cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc ttgcgcgcta
    48481 ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc cgaaaatttc
    48541 taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat cttctaccgc
    48601 tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc gcatcgttgg
    48661 gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg attttggcaa
    48721 aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat cgggaacaat
    48781 aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc cggcgctgtc
    48841 ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct cttcaacgtt
    48901 cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta agggatcaag
    48961 actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg aaaaaccttc
    49021 gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa actgggtaaa
    49081 ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt tatagagcag
    49141 gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt gcagctcgct
    49201 gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat gctcgccagc
    49261 cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg tattctcttc
    49321 cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt ctgccgcttt
    49381 tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg gatcgaacat
    49441 cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc tgatttatca
    49501 gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg gaaaaacatt
    49561 ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat tgaaaccaag
    49621 ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga tgaaacttca
    49681 ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg aaacgcagca
    49741 acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt tggcgctggt
    49801 tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac cggcgaatct
    49861 gccgctgacc attcacgaac tcgaacgtca tccgctgggc attcaggcct ttatcccaat
    49921 gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag acctgtcaac
    49981 gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta acgtctggca
    50041 tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc gcggcggcag
    50101 tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt gacgcctgca
    50161 accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta ggtcggagtt
    50221 aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga gcctgtggca
    50281 cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct ggaaaaaagt
    50341 gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct ctccacgacc
    50401 tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag tcaattaggc
    50461 tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca taaccgcgat
    50521 gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg cgaaacggtc
    50581 aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga gtgtaaatcg
    50641 atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc cggtgcgggc
    50701 aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct gcaaaccggt
    50761 ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa ggacctggaa
    50821 caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg tctgaattgc
    50881 atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc catctccggg
    50941 ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt cagagacacc
    51001 gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct gtcgcatccc
    51061 gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt ccaactaata
    51121 gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat gtgctggaga
    51181 aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg ttctactcag
    51241 cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa ggtgtttcgc
    51301 acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt ctggggactt
    51361 ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct gattccattc
    51421 ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa gattttcagg
    51481 ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca gttcgtgaag
    51541 cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct ggtcgtccgg
    51601 gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag tttgatcctg
    51661 acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag atcgaaaaag
    51721 ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc ggggtaatca
    51781 atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt ccggtgatcc
    51841 caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc gggatggtcg
    51901 gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac atggtgtttg
    51961 gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac accgaagggc
    52021 gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg tgtccggatc
    52081 tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg gcgcaggaga
    52141 tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc cagcagcgta
    52201 aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg cagcgcgtgt
    52261 atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact attggtctgt
    52321 cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg atcaactgtg
    52381 gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt gccgctgatc
    52441 cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg attgaagagt
    52501 tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat aacgcctatc
    52561 tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg caactcgctt
    52621 tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta aaagtagcgg
    52681 aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg ccagcctttg
    52741 aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa gttattctcg
    52801 agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa tttgaagata
    52861 tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat gaataaggga
    52921 gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa tatgattttc
    52981 tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt atgtttcctt
    53041 atgacta
//
LOCUS       DAJTYM010000016        52152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTYM010000016 DAJTYM010000000
VERSION     DAJTYM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..789)
                     /gene="nhaR"
                     /locus_tag="OO575_000964"
     CDS             complement(<1..789)
                     /gene="nhaR"
                     /locus_tag="OO575_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6664021.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVEN"
     gene            complement(855..2021)
                     /gene="nhaA"
                     /locus_tag="OO575_000965"
     CDS             complement(855..2021)
                     /gene="nhaA"
                     /locus_tag="OO575_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6664022.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            2607..2759
                     /gene="mokC"
                     /locus_tag="OO575_000966"
     CDS             2607..2759
                     /gene="mokC"
                     /locus_tag="OO575_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6664023.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(2863..3993)
                     /gene="dnaJ"
                     /locus_tag="OO575_000967"
     CDS             complement(2863..3993)
                     /gene="dnaJ"
                     /locus_tag="OO575_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6664024.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4082..5998)
                     /gene="dnaK"
                     /locus_tag="OO575_000968"
     CDS             complement(4082..5998)
                     /gene="dnaK"
                     /locus_tag="OO575_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6664025.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6375..6779
                     /locus_tag="OO575_000969"
     CDS             6375..6779
                     /locus_tag="OO575_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6664026.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6805..7518
                     /gene="msyB"
                     /locus_tag="OO575_000970"
     CDS             6805..7518
                     /gene="msyB"
                     /locus_tag="OO575_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6664027.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            7667..8233
                     /gene="satP"
                     /locus_tag="OO575_000971"
     CDS             7667..8233
                     /gene="satP"
                     /locus_tag="OO575_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6664028.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8268..8855)
                     /gene="mog"
                     /locus_tag="OO575_000972"
     CDS             complement(8268..8855)
                     /gene="mog"
                     /locus_tag="OO575_000972"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6664029.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(8970..9923)
                     /gene="tal"
                     /locus_tag="OO575_000973"
     CDS             complement(8970..9923)
                     /gene="tal"
                     /locus_tag="OO575_000973"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6664030.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10202..11632
                     /locus_tag="OO575_000974"
                     /pseudo
     CDS             10202..11632
                     /locus_tag="OO575_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            11702..12478
                     /gene="yaaA"
                     /locus_tag="OO575_000975"
     CDS             11702..12478
                     /gene="yaaA"
                     /locus_tag="OO575_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6664031.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(12631..12927)
                     /locus_tag="OO575_000976"
     CDS             complement(12631..12927)
                     /locus_tag="OO575_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6664032.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13141..14427)
                     /gene="thrC"
                     /locus_tag="OO575_000977"
     CDS             complement(13141..14427)
                     /gene="thrC"
                     /locus_tag="OO575_000977"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6664033.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14428..15360)
                     /gene="thrB"
                     /locus_tag="OO575_000978"
     CDS             complement(14428..15360)
                     /gene="thrB"
                     /locus_tag="OO575_000978"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6664034.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15362..17824)
                     /gene="thrA"
                     /locus_tag="OO575_000979"
     CDS             complement(15362..17824)
                     /gene="thrA"
                     /locus_tag="OO575_000979"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6664035.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(17850..17969)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(17905..17970)
                     /gene="thrL"
                     /locus_tag="OO575_000980"
     CDS             complement(17905..17970)
                     /gene="thrL"
                     /locus_tag="OO575_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6664036.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18184..18870)
                     /locus_tag="OO575_000981"
     CDS             complement(18184..18870)
                     /locus_tag="OO575_000981"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6664037.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19270..19410)
                     /locus_tag="OO575_000982"
     CDS             complement(19270..19410)
                     /locus_tag="OO575_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664038.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            19506..20222
                     /gene="arcA"
                     /locus_tag="OO575_000983"
     CDS             19506..20222
                     /gene="arcA"
                     /locus_tag="OO575_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6664039.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20281..21633)
                     /gene="creD"
                     /locus_tag="OO575_000984"
     CDS             complement(20281..21633)
                     /gene="creD"
                     /locus_tag="OO575_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6664040.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(21691..23115)
                     /gene="creC"
                     /locus_tag="OO575_000985"
     CDS             complement(21691..23115)
                     /gene="creC"
                     /locus_tag="OO575_000985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6664041.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23115..23804)
                     /gene="creB"
                     /locus_tag="OO575_000986"
     CDS             complement(23115..23804)
                     /gene="creB"
                     /locus_tag="OO575_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6664042.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(23817..24290)
                     /gene="creA"
                     /locus_tag="OO575_000987"
     CDS             complement(23817..24290)
                     /gene="creA"
                     /locus_tag="OO575_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6664043.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            24501..25370
                     /gene="robA"
                     /locus_tag="OO575_000988"
     CDS             24501..25370
                     /gene="robA"
                     /locus_tag="OO575_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6664044.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25367..26014)
                     /gene="gpmB"
                     /locus_tag="OO575_000989"
     CDS             complement(25367..26014)
                     /gene="gpmB"
                     /locus_tag="OO575_000989"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6664045.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26066..26586
                     /gene="yjjX"
                     /locus_tag="OO575_000990"
                     /pseudo
     CDS             26066..26586
                     /gene="yjjX"
                     /locus_tag="OO575_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(26671..26997)
                     /gene="trpR"
                     /locus_tag="OO575_000991"
     CDS             complement(26671..26997)
                     /gene="trpR"
                     /locus_tag="OO575_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6664046.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27087..29024)
                     /gene="sltY"
                     /locus_tag="OO575_000992"
     CDS             complement(27087..29024)
                     /gene="sltY"
                     /locus_tag="OO575_000992"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6664047.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29235..30902
                     /gene="ettA"
                     /locus_tag="OO575_000993"
     CDS             29235..30902
                     /gene="ettA"
                     /locus_tag="OO575_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6664048.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31009..31176)
                     /locus_tag="OO575_000994"
     CDS             complement(31009..31176)
                     /locus_tag="OO575_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664049.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31210..32442)
                     /gene="nadR"
                     /locus_tag="OO575_000995"
     CDS             complement(31210..32442)
                     /gene="nadR"
                     /locus_tag="OO575_000995"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6664050.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32463..33845)
                     /gene="radA"
                     /locus_tag="OO575_000996"
     CDS             complement(32463..33845)
                     /gene="radA"
                     /locus_tag="OO575_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6664051.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(33894..34862)
                     /gene="serB"
                     /locus_tag="OO575_000997"
     CDS             complement(33894..34862)
                     /gene="serB"
                     /locus_tag="OO575_000997"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6664052.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            34968..35612
                     /locus_tag="OO575_000998"
     CDS             34968..35612
                     /locus_tag="OO575_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6664053.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            35640..36655
                     /gene="lplA"
                     /locus_tag="OO575_000999"
                     /pseudo
     CDS             35640..36655
                     /gene="lplA"
                     /locus_tag="OO575_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            36687..36950
                     /locus_tag="OO575_001000"
     CDS             36687..36950
                     /locus_tag="OO575_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6664054.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37111..37830)
                     /gene="deoD"
                     /locus_tag="OO575_001001"
     CDS             complement(37111..37830)
                     /gene="deoD"
                     /locus_tag="OO575_001001"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6664055.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(37887..39110)
                     /gene="deoB"
                     /locus_tag="OO575_001002"
     CDS             complement(37887..39110)
                     /gene="deoB"
                     /locus_tag="OO575_001002"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6664056.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39162..40484)
                     /gene="deoA"
                     /locus_tag="OO575_001003"
     CDS             complement(39162..40484)
                     /gene="deoA"
                     /locus_tag="OO575_001003"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6664057.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(40611..41390)
                     /gene="deoC"
                     /locus_tag="OO575_001004"
     CDS             complement(40611..41390)
                     /gene="deoC"
                     /locus_tag="OO575_001004"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6664058.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            41648..43189
                     /locus_tag="OO575_001005"
     CDS             41648..43189
                     /locus_tag="OO575_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6664059.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43161..44024
                     /locus_tag="OO575_001006"
     CDS             43161..44024
                     /locus_tag="OO575_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6664060.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44238..45020)
                     /locus_tag="OO575_001007"
     CDS             complement(44238..45020)
                     /locus_tag="OO575_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6664061.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45017..46090)
                     /locus_tag="OO575_001008"
     CDS             complement(45017..46090)
                     /locus_tag="OO575_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6664062.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46212..46373)
                     /locus_tag="OO575_001009"
     CDS             complement(46212..46373)
                     /locus_tag="OO575_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6664063.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46490..47095)
                     /gene="osmY"
                     /locus_tag="OO575_001010"
     CDS             complement(46490..47095)
                     /gene="osmY"
                     /locus_tag="OO575_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6664064.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47488..49077)
                     /gene="prfC"
                     /locus_tag="OO575_001011"
     CDS             complement(47488..49077)
                     /gene="prfC"
                     /locus_tag="OO575_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6664065.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49168..49845)
                     /gene="yjjG"
                     /locus_tag="OO575_001012"
     CDS             complement(49168..49845)
                     /gene="yjjG"
                     /locus_tag="OO575_001012"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6664066.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(49860..50306)
                     /gene="rimI"
                     /locus_tag="OO575_001013"
     CDS             complement(49860..50306)
                     /gene="rimI"
                     /locus_tag="OO575_001013"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6664067.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50275..50688)
                     /gene="holD"
                     /locus_tag="OO575_001014"
     CDS             complement(50275..50688)
                     /gene="holD"
                     /locus_tag="OO575_001014"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6664068.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            50791..51822
                     /gene="rsmC"
                     /locus_tag="OO575_001015"
     CDS             50791..51822
                     /gene="rsmC"
                     /locus_tag="OO575_001015"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6664069.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12379 a        14133 c        13094 g        12546 t
ORIGIN      
        1 attctcgacg cgaccaattt ctacgacagt tttatcggca taaaagtcat atgcataaag
       61 cgttggggca acgaagattg cattgtgcat cgcaccaaaa gctttcatca aagcggcatc
      121 atcaaactcg ccgaggattt ctacgtttaa tccctgggag ttaaaccagt taagcaattt
      181 gcgccctaac attgaacgtc gcccaggaat caaaagtcgc cgttcttcca gacaagccgg
      241 gaacggtttt tctggtggtg gatttgtaca ccagaaactc acgccacatt cgccaattct
      301 cacggagaac aggccttcct gctgcgtaga gtctatcgga cagtcagaaa tgatcatatc
      361 cagtttatgc tgacttaatt gctccagcag catttcgtgg gtggattcga agcagcgaag
      421 atgaatgggc tcgccttcta ccactgcggc gttaagtacg ctactgacca ggcgtttgga
      481 aagtgcatca gccacgccaa cgtcaaacaa taaattggat tctttgcgat agttcacaat
      541 atccagcatt tcctggctta aggtgaacat tttatcggca tagcgataga ccagttctcc
      601 cagctcgctg ggttcgagac cacgtccctt gcgtttaaat aatttgcctt gcaggcgctc
      661 ttccagcgct cgaatctgtc cggtaatggt ttgtggagtt aaataaagcg cctccgctgc
      721 gccaaccacg gaaccttctt tatagacatg ccagaagtaa tacaagtggt tgtaattgat
      781 atgagacatg ctcatttctc tccctgataa caatgaaaag ggagccgttt atggctcccc
      841 ggtaaaccgt cctgtcaaac tgatggacgc aaacgaacgc gtaaccagct gtatccaatt
      901 accgccgaag agatagaacc gaccaggata ccgagtttcg cccagttaat cagttctgga
      961 tctacgctac caaaggccag gctggcaata aagatagaca tagtaaaacc gataccgcac
     1021 aggatcccca ccaccataat ttgctgataa gtcgttccct caggcagatg cgccagtttc
     1081 aaacgcagcg ccaaccagca gaacagacta atccccagcg gtttgccaat cagcaagcca
     1141 gcgatgatcc ccaatggcag aatggaggtc aagccatcca gcgtgacgcc ttgcagtgaa
     1201 acgccagcat tagcaaatgc aaacagcggc aaaatcagat acgccaccca cgggtgcaac
     1261 acatgctcca gtcgcttcgc tggagaacgc ccatgcttct ctttcaaagg aataaagaag
     1321 ccgacaatta cccccgccag agttgcgtga acccccgatt tcaacaccgc agtccacaac
     1381 accacgccaa caagaatata gacgcccgtg cggcgtacac cacacagatt caataccgcg
     1441 agtaccgcaa ttgctacagc cgcgacgcca agggaggcca tcgataagtc attagtgtag
     1501 aacaatgcga tgataatgat ggccccaaga tcgtcgataa tagccagagc catcaaaaag
     1561 atcttcagca ctaacggaac acgacttccc aacagcgcca gtacaccaag tgcaaaagca
     1621 atgtcagtag ccgccgggat cgcccaccct tcgcgggtaa tcggatcggc atagttaaaa
     1681 gccagataga gtaatgccgg cacaatcatc ccaccaatag cggcgataac tggaaatgcg
     1741 gcctggcgta agctggctag cgatccttgc atcagttcac gtttaacttc cagaccgact
     1801 aacaggaaaa ataccgccat cagcgcatca tttatccata acagcatgtt tttgttgatt
     1861 tcgagtgaac caacccggag ctgaaccggc gtctccagaa agtcgtgata ccatccactg
     1921 gttgcgccgc tgttggccat aatcatcgcc aggatagcgg caatgataag aataatgcct
     1981 cccgaggcat cactgctaaa gaatcgatgc agatgtttca ctttttattt ctctttcagg
     2041 tgaatagatc gtttttacta ttttactccg cacgaatcat cgttaaaatc agattatagt
     2101 gtaggaataa ttcgtttttt acgagccatt gaagaatttg cgccatcagc ggtgagatgc
     2161 gtttttgacg gctgtaagcg ctcacgagtg accctaagcc cagataatca tagcttaagg
     2221 tgaggattcc tgagggatta attaggaaaa gtaataatta atttgttgtc agtcggcgat
     2281 gcttgttagg ccaatatttc aaaaataatc cagcttgtcg tcaataattg cgatgaagag
     2341 ataaattaaa gttaaataat taaatcatta aaaaattacg ccacaaaaat gcgaggcgtc
     2401 ttcaggatta gaatcgataa aacaaaatgg gttaaagaga gcgatataat agcggcgggt
     2461 gcttgaggct gtctgtctca ggcattagct gaacggcaga tagagaaaag ccccgagtga
     2521 tattttacca tcaacccgag gcctcctata tgctgaacac atgtagagtg cctcttactg
     2581 accgtaaggt caaggagaag agagcaatga agcagcataa ggcgatgatt gtcgccctga
     2641 tcgtcatctg tatcaccgcc gtagtggcgg cgctggtaac gagaaaagac ctctgtgagg
     2701 ttcacatccg aactggccag acggaggttg ctgttttcac ggcttacgaa tccgagtaag
     2761 agcaacggcg gggagtgatc cccgccactt tacaggtgct cgcatatctt caacgcaccc
     2821 tatttttacc caggcctgcc cacgggcagg cttttgggga ggttagcggg tcaggtcgtc
     2881 aaaaaacttc ttcacaccat caaagaagct ctttgagcgc gggctgttgt gctcgccggt
     2941 tgggccaccg aagctttctt gcagctcttg cagcagctgt ttctgctttt cgttcaggcc
     3001 taccggtgtt tcgacgacaa cgcggcacag caaatcaccc tgtgcgccac cgcggacaga
     3061 cttgacgcct ttaccgcgca tacggaatag cttaccggtc tgggtttcgc caggcacttt
     3121 cagtttgacg cgaccatcaa gggtcggtac ttcgatttcg ccacccagcg ccgccatagc
     3181 gaagttgatc gggacttcgc aatacaggtt gttgccttca cgctcgaaaa tcgggtgctg
     3241 tttaacctga acctgaacgt acagatcgcc tgccggtgcg ccatgctcgc ccgcttcacc
     3301 ttcgcccgca agacggatgc ggtctccagt gtccacccct gccgggattt taacggacag
     3361 cgttttgctg cgctcaacac gaccatgacc atgacatttg ttgcacggat ctttgatcag
     3421 cgtaccgcgg ccctgacagt gtggacaggt ctgctgtaca gcgaagaatc cctggcgcat
     3481 ctgcacctga ccagaaccat gacaggtcgg acaggtctgc ggctgtgtac ctggttttgc
     3541 accgctaccg tggcaaacgt cacactcttc cagagtcgga atgcggatct ctttggtcac
     3601 gccacgtaca gcttcttcga gggtgagctc catgttatag cgtaaatcag caccgcgcgc
     3661 cgcacgttga cgaccacgtc cgccgccaaa aatatcgccg aaaacgtcac caaaaatatc
     3721 gctgaagtct gcgccgccgc caaaaccacc gccgcccatg cctccttgct caaacgcagc
     3781 atgaccatac tgatcgtatg ccgcacgttt ttgcgagtcg gtcagaactt cataagcttc
     3841 cttgatctct ttaaatttcg cctcggcctc tttgtcaccc tggttacggt ccgggtggta
     3901 tttcatggcc aggcgtttgt aggcctttct gatttcacgc tcttccgctg ttttggaaac
     3961 gcctaaaatc tcgtaataat cttgcttagc catctttttt aaattgcccc tagatgaatg
     4021 cacgggcgga gaggaaattc cccttcgccc gtgtcagtat aattacccgt ttatagggcg
     4081 attatttttt gtctttgact tcttcaaatt cagcgtcgac aacatcgtca tctttcgcgt
     4141 tgtttgcaga agcatcagca ccggcagtct gctgctgggc atgttgctgc tgggcgattt
     4201 ccatcagttt ctgggaaacc tgtgccagtt cctgcatttt cgcttcgata gcggctttgt
     4261 cttcaccttt cagagcagtt tccagtgcag tcagcgcaga ctcgatagca gttttgtcgt
     4321 cagccggcag tttgtcgcct gcttcttcaa cctgcttacg ggtgctgtgc agcagatggt
     4381 cgccctggtt gcgagtctgt accagctctt caaacttacg gtcagcttcg gcgttagctt
     4441 ctgcgtcgcg taccattttc tggatttcat cttcgttcag accagaagaa gccttgatgg
     4501 tgatcttctg ctctttaccg ctgtttttat ctttcgcgga aacgtgcagg ataccgtcag
     4561 catcgatatc gaaggtaact tcgatctgcg gcatgccgcg cggtgccggg ttgataccat
     4621 ctaggttgaa ctgacccaga gatttgttat cagccgcacg tttacgttca ccctgcagca
     4681 catggatggt taccgcagac tggttgtctt cagcggtaga gaacacctgg ctgtgcttgg
     4741 tcgggatagt ggtgtttttc gcgatcagcg tcgtcatcac accgcccatg gtttcgatac
     4801 ccagagacag cggggtaacg tccagcagca gtacgtcttt tacgtcacca gtcagaacac
     4861 caccctgaac agcagcaccg attgctacag cttcgtccgg gttaacgtct ttacgcggct
     4921 ctttaccaaa gaactcagca actttcttct gaaccattgg catacgagtc tgaccaccaa
     4981 cgaggataac gtcgtcgata tcagatacgg acaggccagc atcctgcagt gcaactttca
     5041 gcggctcaat ggaacggttt accagatctt caaccaggct ttccagtttc gcacgagtca
     5101 ctttgatgtt catgtgtttc ggaccggtcg cgtctgcagt gatgtatggc aggttaacgt
     5161 cggtctgctg agcggaagac agttcgattt tcgctttttc tgccgcttct ttcaggcgct
     5221 gcattgccag cggatcgttg cgcaggtcaa tgccctgatc tttcttgaat tcttcaacca
     5281 gatagttgat cagacggctg tcgaagtctt caccacccag gtgggtatca ccgttggttg
     5341 ccagaacttc gaaggttttt tcgccgtcaa cttcgtcgat ttcgataata gaaatatcga
     5401 aagtaccacc ccccaggtca taaaccgcga tagtacggtt gccagtgcct ttgtccagac
     5461 cgtaagccag cgcagctgcg gtcggttcgt tgatgatacg ttttacttcc agaccagcga
     5521 tacggcctgc gtctttggtt gcctgacgct gagcatcgtt aaagtatgcc ggtacggtga
     5581 taacagcttc agttaccggt tcacccaggt aatcttcagc ggttttcttc atttttttca
     5641 gcacttcagc agaaatctgc ggcggtgcca ttttctggcc tttaacttcg acccatgcgt
     5701 cgccgttatc agcagcaata attttgaacg gcatgatgga aacatcacgc tgtacttctt
     5761 cgtcctggaa gcggcggcca atcaggcgtt taatcgcaaa cagggtgttt tgcgggttcg
     5821 tcactgcctg acgtttagcc ggctgaccaa ccagagtttc accatcctgg gtataggcaa
     5881 tgatagaagg cgtggtgcga tcgccttcgg cgttctccag cacgcgagga atggtgccat
     5941 ccataatcgc tacacaagag ttggtagtac ccaggtcgat accaattatt ttacccatct
     6001 aaacgtctcc actatatatt cggtcatcat gtggttgtga gtctgtaata ggggcgaaac
     6061 gtctggtttc aactgcccaa tttcattttt ttgcagactc actcactgcg gttgactact
     6121 aaatggggtc gtaacccacg tcatcaaggg ggagatgcaa aaattttttg tgcttttgcg
     6181 cagaaatttc gcgatatggc aggacaagac gtaaaattga cagcaatttt attcatataa
     6241 agaatgaatc gcagccaagg cgttcatcaa aaaattgtaa taaaaagaaa agattacgtg
     6301 cctgaatctt ctctttatca gcagtaaact agtgggtatt catcccccta cctcttccca
     6361 ctaagagaat ccttatgaaa tccgttttta cgatttccgc cagcctggcg attagcctga
     6421 tgctgtgctg cacggcgcag gcaaacgacc ataaactcct cggcgtcatt gcaatgccgc
     6481 gtaacgaaac caacgatctg gcgctgaaac ttcctgtttg tcgcattgtg aaacgcatac
     6541 aactctctgc cgaccatggc gatttacagt taagtggtgc atcggtttat ttcaaagccg
     6601 cccgtagcgc cagtcagagc ctgaatattc cttcagaaat aaaagaaggg caaaccactg
     6661 actggatcaa cattaacagc gataacgaca ataaacgctg cgtctcaaaa atcacctttt
     6721 ccggtcatac ggtgaactca tcggatatgg ccacgctgaa aattatcggc gacgactaac
     6781 gccagaatat agaagccaca aaaaatgaat gttaattacc tgaatgattc agatctggat
     6841 tttcttcagc attgtagtga ggaacagttg gcaaatttcg cccgattgct cacccataat
     6901 gaaaaaggca aaactcgcct ctccagcata ctgatgcgca acgaactgtt taaatcgatg
     6961 gaagggcatc ccgagcaaca tcgccgcaac tggcagctga ttgccggaga attacagcat
     7021 tttggtggcg atagtatcgc caacaaactg cgcggacacg gtaaattgta tcgggccatt
     7081 ttgctcgatg tttcaaagcg attgaagctg aaagccgaca aagagatgtc tacgtttgaa
     7141 attgagcaac agttactgga acaatttctg cgtaatacct ggaagaaaat ggacgaggaa
     7201 cataagcagg agtttctgca cgcggtcgat gccagggtga atgagctgga agagctgctg
     7261 ccgctgctga tgaaagacaa attattggca aaaggtgtgt cgcatttgct ttccagccaa
     7321 ctgacccgca ttttacgcac ccacgcagca atgagcgtac ttgggcatgg tttgctgcgc
     7381 ggcgcggggc tgggaggccc tgtgggcgca gcattaaatg gggttaaagc ggtcagcggc
     7441 agcacctatc gcgtgacgat tccagccgta ctgcaaatcg cctgcctgcg ccggatggtt
     7501 agcgccactc aggtctgata ccggaaaccg gggaaaattt tcattttccc ctcaaaagat
     7561 cgtagacact gccccactgg ctgattatta tgccgcgccc tgaaaacact acagttattc
     7621 agggaaatta tttcaccatt cattcgatga tgatttttga ggaattatgg gcaacactaa
     7681 gttggctaat ccggcaccgc tgggcctgat gggcttcggc atgaccacca ttctgcttaa
     7741 cctgcacaac gtgggttatt tcgctctgga cggtattatt cttgccatgg gcattttcta
     7801 cggcggcatc gcgcaaattt ttgctggtct gctggagtac aaaaaaggca acactttcgg
     7861 tttaaccgca ttcacctctt acggttcttt ctggctgacg ctggttgcga ttctgctgat
     7921 gccgaaactg ggtctgaccg atgcgccaaa tgcacagttc cttggtgtct acctgggtct
     7981 gtggggcgta tttacgctgt ttatgttctt cggcactctg aaaggcgcgc gcgtcctgca
     8041 attcgttttc tttagcctga ccgtgctgtt tgccctgctg gcgatcggta acattgccgg
     8101 taatgccgcg atcatccact ttgccggctg gattggtctg atctgcggtg ccagcgcaat
     8161 ctatctggcg atgggtgaag tgctgaacga gcagtttggt cgcaccgttc tgccgattgg
     8221 tgaatcccac taattgtctt gagatccccc cgctcggggg gattttttta ttcgctaacg
     8281 tcgcgtcttg cactcttcgg tctgaatgca gcaaccactt ctggtgccgt ttcaacgtat
     8341 ggcccttcca gcaactgaat gcagtacggt acgctggcaa aaataccatg caccacaacg
     8401 ttaccctcag cgtccttcac accttccagc gtctctttaa tagacttcgg ctgaccgggt
     8461 aagttaagga tcagcgcctg tttgcgaatc acgcccacct gacgcgaaag gatcgcagtt
     8521 ggtacaaaat gcaggctgat ctggcgcatc tgttcaccaa agccaggcat ctcgcggtcc
     8581 gctactgcca gcgtcgcatc gggcgttacg tcacgacgcg ccgggccagt tccgcccgtg
     8641 gtgagcacca gatggcaact catttcatcc accagctcac acaacgtttg ctcgatgatc
     8701 gcctgctcat cggggattaa gcgggtttcc agttcaaacg gcgtggttag cgccgatgtc
     8761 agccattctt ccagcgctgg aatgccttta tcctgataaa cgccgctgga tgcgcgatca
     8821 gagatggaaa ctaagccaat acgtaaagta ttcatcaaga tttttccgtt taaacaatct
     8881 cgttaaacag aatgatacac tgcgaaggga gtgacagaca ggcttcagaa gaggtagcgt
     8941 gaccgacttc ccggtcacgc taagaatgat tacagcagat cgccgatcat tttttccagt
     9001 ttttcctggt caatagcaaa cttacggata ccttccgcca gtttatctac tgccattgga
     9061 tcctggttgt gctgccacag gaactcggac tcagtgatac gcgccggacg cgctttcact
     9121 tcgccggtgt aagacagttt acgttcgata gccccttcgc tctccgccag ctctttcagc
     9181 agtgccggtg cgatggtcag acggtcacag cctgccagtt ccagaatttc gccgatgtta
     9241 cggaagcttg cgcccataac cacggtttca taaccgtgct ctttgtagta ctggtagatt
     9301 tcagatacag aaaccacgcc cggatcttct gccggagcgt actctttctt atcggtattc
     9361 gctttgtacc agtcaagaat acggccaaca aacggcgaga tcaggaacac gcccgcctcc
     9421 gcacaagcac gagcctgagc gaaggagaac agcagggtca ggttacagtt gataccttct
     9481 ttttccagct gttctgcagc acggataccc tgccaggtag aagccagttt gatcagaata
     9541 cgatcgttgc taatacctgc atcgttgtag agtttgatca ggcgttttgc tttcgcaatt
     9601 gacgcttcag tgtcatagga aagacgcgca tcaacttcag ttgagatacg gcccggaacc
     9661 agtttcagga tttccagacc aatatttact gccagtttgt cggtcgcgtc cacgatctgc
     9721 tgcgcgcgat cgttgctctg ctgtttcgcc caggcgacag catcatcaat caacttacgg
     9781 tattccggaa tctgcgctgc gttaagaatg agagaagggt tggttgtggc atcctgcggt
     9841 tgatacagct tcattgccgc gatgtcccca gtgtcggcca ctacggtggt gtactgacga
     9901 agggaggtca atttgtccgt catgatagta tttctcttta aacagcttgt tagggggatg
     9961 taaccggtct gccctgatga tatcacgacg caacgtcagc gcaaccgccg acaagacggt
    10021 tatcgccagg cgagactgtt tcggatttct gacactgcgt tgatatcgcc caccattttt
    10081 atacaaaacc tcatgtatgc tacgcagaag ttatcaagta cctcgtagcg tatatacttc
    10141 ttaaacaatt ggtaacgttt acacaggaaa gtcatcgcga ccggcaataa gagggatatg
    10201 catgccagat tttttctcct ttattaacag cgtcctttgg ggatcggtaa tgatttacct
    10261 gctcttcggc gcaggttgtt ggttcacttt tcgcaccgga tttgtgcagt ttcgctacat
    10321 ccgccagttt ggcaaaagtc ttaaaaatag cattcatcca cagccaggcg gtttaacctc
    10381 atttcagtca ttgtgtacca gtcttgcggc gcgcgtgggt agcggcaacc tggccggcgt
    10441 tgcgctggca attaccgccg gtggaccagg tgccgtcttt tggatgtggg ttgctgcgtt
    10501 tatcggcatg gcgacctcgt ttgccgaatg ttcccttgca caactttata aagaacgtga
    10561 cgtcaatggg cagtttcgtg gcggaccggc atggtatatg gcgcgcgggc tggggatgcg
    10621 ctggatgggc gttctgttcg ccgtcttttt gctcatcgcc tacggcataa ttttcagcgg
    10681 agttcaggcg aacgccgttg cccgagccct gagtttttct tttgattttc ctccgctggt
    10741 gacgggcatt attctcgctg tctttgctct gctggcaatc actcgtggtc ttcatggcgt
    10801 cgcccggctc atgcaggggt ttgtcccgtt gatggcgata atctgggtac tgaccagcct
    10861 ggtgatttgc gtaataaata tcgggcaact tccccacgtc atttggtcta tttttgagag
    10921 tgcttttggc tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat
    10981 tactaacggt tttcagcgca gtatgttttc caatgaggtg ggaatgggat cgacgccaaa
    11041 cgcggcagcg gcagctgcat cctggcctcc gcatccggca gcacaaggga ttgtccagat
    11101 gattggcatt tttatcgaca ccctggtcat ctgtacggca agcgccatgc tgatattact
    11161 ggcgggtaac ggcacaacct acatgccgct ggaaggtatt cagcttatcc agaaggcgat
    11221 gcgggtgcta atgggttcct agggtgctga atttgttacc ctcgtggtta ttctgtttgc
    11281 cttcagctcc atcgttgcca actacattta tgccgaaaac aatctcttct ttttacgcct
    11341 gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg
    11401 caccttgcta agccttccgc tgatgtggca actggcagat atcataatgg cctgcatggc
    11461 tattaccaat ttgaccgcca ttttactgct ctcgcctgtg gttcatacca ttgccagtga
    11521 ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc gttatccgga
    11581 tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact
    11641 atcgatcaac tcattctctt tttttgctaa agtcggcata aatttcctgc aaggactgga
    11701 tatgctgatt cttatttcac ctgcgaaaac gcttgattac caaagcccgt tgaccaccac
    11761 gcgctatacg ctgccggagc tgttagacaa ttcccagcag ttgatccatg aagcgcggaa
    11821 actgacgcct ccgcagatta gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa
    11881 cgccgctcgc tttcatgact ggcagccaga tttcacgccg gcgaatgccc gccaggcgat
    11941 tctggcgttt aaaggtgatg tctacaccgg cttgcaggcc gaaaccttca gcgaagacga
    12001 tttcgatttt gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactgcgccc
    12061 gctcgattta atgcagcctt atcgtctgga aatggggatc cgtcttgaga atgcccgagg
    12121 gaaagatctg tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc
    12181 agcacaaggc gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa
    12241 gccgaagaaa ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa
    12301 atttaagatc atcagcttct acgctaagaa agcacgcggt ctgatgagtc gtttcattat
    12361 tgaaaatcgg ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt
    12421 tgatgaagat tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt
    12481 ttgtagtcaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgcagatt
    12541 caatatattg cagaattaac gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg
    12601 aatgccggat tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc
    12661 gccgtgatga tcatgaggag ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg
    12721 gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg
    12781 acctccatcc cagtaatagc cacgattatc acgatcgcct atctgtaatt ttactgacgg
    12841 gactaacgta atttccgcag cctgtgctgc catgggagcg accagaacca gggaaagtgc
    12901 gagtacgata gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt
    12961 cgatatccgg agattacgcg atgagaacga cggttgttat tctccgcaat cctaatctct
    13021 aagttaccgc atttattgat aatttctcct ttttccctgt cgtttttctc cataatttct
    13081 tcataaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagatt
    13141 ttactgatga ttcatcatca atttacgcaa cgcagaaaaa tcggcgggca gattatgtga
    13201 aagcaagggt aaatcagcac gttctgccag ctcttttggc agatccaacg tttcaccgag
    13261 aatcgcttcc acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata
    13321 ttcgcctgga ttcaactggt cacgcagcgc acgatacgct acggcagcgt gcggctccga
    13381 ggtgtagccc agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc
    13441 tgcataaccc agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg
    13501 ccagttgttc ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg
    13561 tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat
    13621 aaaacgtttc accggcagac cgaatgactt cgccagcaga cccgccgtca aatcgccgaa
    13681 gtttccgctt ggcaccgaga caaccagctg gttgcgcgcc tcctgcggca gctgcgcaac
    13741 agcttcaaag tagtagcaaa tctgcgccag caaacggctg atgttaatcg agttagccga
    13801 gtttaacccc agcgccactt tcagttcttc atcatcaaac gcctgcttca ccagcgcctg
    13861 acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca acgtacagaa
    13921 cagtttttct tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt
    13981 cggcaaaccg tagaaagcat gagccactgc agctccggta tcaccggagg tcgcggtcag
    14041 aatggtcact ggcttatcat ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc
    14101 gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc
    14161 aacattggcg accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc
    14221 ctgtgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc
    14281 cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcgt gcggaaaaaa
    14341 cagcccctga tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcgtt
    14401 gtgatctttc agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc
    14461 cagccggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc
    14521 ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg aacaaggtcg ggccggagcc
    14581 ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc
    14641 aggcagtaac cgttcacggt agggttcagc gataacatct ttcatcagct tcgcggcaag
    14701 ctcaggctga cgggaatagc aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat
    14761 gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg tcgagacttt
    14821 aattcccgga tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat
    14881 gatgtcgttt tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc
    14941 gtaatgaatg ctgccggaga tacgtccttc cagctcgccc atcaaagcca gcaaacgagt
    15001 gtcattaagc ggcttgccgc agtgttcatt catcgccatc agcgccgcga ccaccgaaca
    15061 ggcgctggag cctaagcccg aaccgatcgg catattcttt tccagagtca tcgccactgg
    15121 aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac tgataaacga tattttcccg
    15181 tggttctgac ggcagcttat cggcaaagcg tccgagtttg ttgagactga atgtctctgc
    15241 cgcctcaacc gtgactacat ctccgagcaa tgcaccatca acgggtgcca ccgccgcccc
    15301 gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat
    15361 gtcagactcc taacttccat gagagggtac gtagcagatc ggcaaagaca ccggcagcgg
    15421 taacgtcatt gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc
    15481 tataaaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca ttcccatcca
    15541 cttcggcaat cttcacgcgg cagacaccat cttcatcaat attgccaaca tagcgcaaaa
    15601 cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca
    15661 gattcgccat aaaagcggca acatcaccct cggcgttaaa ctctgcgggc agcacaggtt
    15721 caatttcaat atccgccagc tccagttcac gtcccgtttc acgagcgaga atcaatagtt
    15781 tacgcgccac atccatacca gaaagatcat ctcgcgggtc cggttcggta taacccattt
    15841 cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat
    15901 aagaaagcga acctgaaaga atgccggaga acttcaccaa ttcatcacct gcattgagca
    15961 gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt
    16021 tacgccgcga tttttccgcc gcataacgca actgatgata gtaatccatc gacgaggtgt
    16081 tggccttttt gttcggcgtg acaacgtgga aaccttcgcg caggaagtcg gcatattgat
    16141 ccgccactgc ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt
    16201 tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgtgca agttcttcct
    16261 gccagttttc cagattaagg ccatgcacac tagtgagcag agccttcgag ttggcaacgc
    16321 cgcagacacg taagtcgata tgtttattct tcagccagct ttgctgacgc ttcagttgct
    16381 ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg
    16441 tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca
    16501 cgacagagat tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc
    16561 gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta
    16621 ccaccgagat aatggccagc cgttccgtca ccgccagcgg ctccagtaag ccttctttca
    16681 gttccaggta gaactcttcc tgcattgccc gttcagctcg cacacagtcg ctttgtggaa
    16741 cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg
    16801 cgcgtgacat tgcagcaaag acgcgcgccg ccatgccaac catccctttc atccccgggc
    16861 cggaaacgct gaacattgcc atgttattca gattggaaat gcccttgacc ggtaattcgt
    16921 cttcatcacg gctggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa
    16981 tcaggcaagg gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag
    17041 cgccgaagta ggaaagctcc atcgcttcct ggtaggacat cgacttcaac aacctcgcat
    17101 cgggcacctg acgcgggtcg caggtataga ccccgtcaac gtccgtccaa atctcgcaac
    17161 aatcggcgcg taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttacgtccaa
    17221 gcaccaccag ttcgcctttt tcattaccgg cggtgaaacc tgccatcaac accatgtgat
    17281 cagccggaat gcggcttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt
    17341 cgaggtaatg ccccactgcc agcagttttt cgaccggatc gataacggta acgttgtgac
    17401 cacgcgcttc taacacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca
    17461 gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact aatgccatgc aggacatgtt
    17521 ttatttgggc aaattcctga tcgacgaaag ttttcaattg cgccagcggg aaccccggct
    17581 gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg
    17641 gtaaagcatc ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt
    17701 tggcgggggc agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat
    17761 cggcaacacg cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc
    17821 gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc
    17881 ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt
    17941 ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa tttttatctg
    18001 tttgtgcgct atgcctatat tggttaaagt atttagtgac ctaagtcaat aaaattttaa
    18061 tttactcacg gcaggtaacc agttcagaag ctgctatcag acactctttt tttaatccac
    18121 acagagacat attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt
    18181 tttttatttg gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc
    18241 tcgttgctct aaaagcccca ggcgttgctg taaccagtcg accagtttta tgtcatctgc
    18301 cactgccaga gtcgtcagca atgccatgac tcgttcgcgt aaagcttgca gttgatgttg
    18361 gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat gttgctaatt gatagcaata
    18421 gaccatcacc gcctgcccca gattgagcga aggataatcc gccaccatcg gcacaccagt
    18481 aagcacgtca gccaacgcta actcttcgtt agtcagcccg gaatcttcgc gaccaaacac
    18541 cagcgcggca tggctcatcc atgaagattt ttcctctaac agcggcacca gttctactgg
    18601 cgtggcgtag taatgatatt tcgcccgact acgcgcagtg gtggcgacag tgaaatcgac
    18661 atcgtgtaac gattcagcca gtgtcgggaa aactttaata ttatcaataa tatcaccaga
    18721 tccatgtgcg acccagcggg tggctggctc caggtgtgcc tgactatcga caatccgcag
    18781 ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc ccaatatttt ctgctctggc
    18841 gggtgcgacc aggataatcg ttatacgcat attgccactc ttcttgacca aataaccgcg
    18901 aacagggtga tcactgtcaa cttattacgc ggtgcgaatt tacaaattct taacgtaagc
    18961 cgcagaaaaa gccctttact tagcttaaaa aaggctaaac tattttctga ctgcactaac
    19021 ggtttaattg ttaaaaaatg ctacatatcc ttctgtttac ttaggataat tttataaaaa
    19081 ataaatctcg acaattggat tcaccacgtt tattagttgt atgatgcaac tagttggatt
    19141 attaaaataa tgtgacgaaa gctagcattt agatacgatg atttcatcaa actgttaacg
    19201 tgctacaatt gaacttgata tatgtcaacg aagcgtagtt ttattgggtg tccggcccct
    19261 cttagcctgt tatgttgctg ttaaaatggt taggatgaca gccgtttttg acactgtcgg
    19321 gtcctgaggg aaagtaccca cgaccaagct aatgatgttg ttgacgttga tggaaagtgc
    19381 atcaagaacg caattacgta ctttagtcat gttacgccga tcatgttaat ttgcagcatg
    19441 catcaggcag gtcagggact tttgtacttc ctgtttcgat ttagttggca atttaggtag
    19501 caaacatgca gaccccgcac attcttatcg ttgaagacga gttggtaaca cgcaacacgt
    19561 tgaaaagtat tttcgaagcg gaaggctatg atgttttcga agcgacagat ggcgcggaaa
    19621 tgcatcagat cctctctgaa tatgacatca acctggtgat catggatatc aatctgccgg
    19681 gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt
    19741 tcctgactgg ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg
    19801 actacatcac caaaccgttc aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt
    19861 cccgtaccat gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa agctacaagt
    19921 tcaatggttg ggaactggac atcaacagcc gttcgttgat cggccctgat ggcgagcagt
    19981 acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa
    20041 ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa ccgcacgacc
    20101 gtactgtaga cgtgacgatc cgccgtattc gtaaacattt cgaatctacg ccggatacgc
    20161 cggaaatcat cgccaccatt cacggtgaag gttatcgctt ctgcggtgat ctggaagatt
    20221 aatcggcttt accaccgtca aaaaaacggc gctttttagc gccgttttta tttttcaacc
    20281 ttatttccag atacgtaatt catcgtccgt tgtaacttct ttactggctt tcattttcgg
    20341 cagtgaaaac gcataccagt ctatattacg agtcacaaac atcatgccgg ccagcgccac
    20401 caccagcaca ctggttccca acaacagcgc gctatcggca gagttgagca gtccccacat
    20461 cactccatcc agcaacaaca gcgcgagggt aaacaacatg ctattgcgcc agcctttcaa
    20521 taccgcctgc aaataaatac cgttcattaa cgccccaatc agactggcga ttatccatgc
    20581 cacggtaaaa ccgatatgtt cagaaagcgc cagcaagagc aaataaaaca tcaccaatga
    20641 aagccccacc agcaaatatt gcattgggtg taaacgttgc gcggtgagcg tttcaaaaac
    20701 aaagaacgcc ataaaagtca gtgcaatcag cagaatggcg tacttagtcg cccggtccgt
    20761 taattggtat tgatcggctg gcgtcgtcac tgcgacgcta aacgcgggga agttttccca
    20821 gccggtatca ttgcctgaag caaaacgctc accgagatta ttagcaaacc agctgctttg
    20881 ccattgcgcc tgaaaacctg actcgctagc ttcccgtttg gctggtagaa aatcacctaa
    20941 aaaactggga tgcggccagt tgctggttaa ggtcatttcg ctattacgcc cggcaggcac
    21001 cacagaaaga tcgccggtac cgcttaaatt cagtgccata ttcagctgca ggttctgctt
    21061 ccgccagtcc ccttcgggta aagggatatg cacgccctgc ccgccttgct ctaacccggt
    21121 accgggttca atggtcagtg ccgttccgtt aacttcaggc gctttcacca caccaatacc
    21181 acgcgcatcc ccgacgctaa tcacaataaa gggcttgcct aagatgatat ttggcgcgtt
    21241 gagctcgcta agacgcgaaa catcgaaatc ggcttttagc gttaaatcac tgtgccagac
    21301 ctgaccggta taaatcccta tcttgcgttc ttccacgttc tgattgccat caaccatcaa
    21361 tgactcaggt aaccaaaaat ggataaaact tcgtttccgc tccacggttt tatcctcttc
    21421 ctgcaccgta taaagctcgg tcacaggaat agcgataagc ggcccaacga gtttttgcgg
    21481 cccgctggta ctttggcgaa tcgcatcttc cacatcgcta cggtaatcgg cacgttcgac
    21541 aatcacttgc cgaatcagca ttatcggaat caacaacagc aatactgcac caaacaaggt
    21601 ggtcattttc cagaacaggg gggatttcaa catagtcttc tcctttgcaa tggcagcagg
    21661 atacgaaggc tatgtgggaa gaatttgaag ctatgtgaag tgacggtgaa gtcgtagcga
    21721 ggccagcacg ccaccttcct gcacgttgcg cagcgtgact tcgccgttaa acaaacgggc
    21781 gacctcactg acgaacgcca accccagacc gctgcttttt tgcccatttg cacgcggcaa
    21841 agagtaaaag cgttcaaaaa tacgtgacag cgcgtagtca ggaataccac taccggtatc
    21901 cagcacctta agcgtgacgt gttcctgatc cacttcggcg cttagcgtta tgcaaccgct
    21961 ctcgggagta aaatcgatgg cgttatccag taaattcccc agcgcctgat ccagtaacgc
    22021 cggttcagca gcaacattaa cctcggtggg cataacatgc aaagtgatgt ttttttctgc
    22081 caactgcacg gtgcgcgctt cgctgacgcg gcgaaataat gccgccacat caacagcagt
    22141 cagaacgact tcctgacgat tctccagtct tgcctggcgt agtaacgttt ccaccagcgc
    22201 ctgcattcgc gcattttgtg tcagaatgtt gtcagtaaaa cgagccacta cttcaggcgg
    22261 cggaccttcg cgtaaaattt ccgccgcgcc acgaatcgcc gccagtgggc tttttagctc
    22321 atgggttaac gcataaacat actgctcaat atagtttttc ccttccagct tcacgcgcat
    22381 actttccagc gcctgcgcga gtttacgcag ctcgctacta ccgagatcgg gaagaggaac
    22441 gggcttattg tcagtgacgg aatcagcata gcgagtgagc cgggcaatgg agcggttgat
    22501 ccaccaaacc atgcctgcgc caatcaccag tgcaatcccc aacaaaatgg cgctggccca
    22561 taaaattcgc cgctcgctgc gtttaatgac cggagccatc gccgcgttcg gtttgcctac
    22621 gctcaaaacg ccaataagcc gcgagccgcc cataatcggc gcggcaacat acatcaccga
    22681 actttcggga tcggcaggat tttgcaacgt gctgcgcgca ccatactgac cacgcaacgt
    22741 tagccagacg tcattccagc gcgaataatc ctgtccaacg gctttatttg ccgaatcgaa
    22801 caatactttg ccatgcgcat cggtcatata gacatgatat tcattgcgca ctttgttaat
    22861 gccaccaata ttggcgcgaa acgggcgatg ttgtagctga ttaaacgcct gcgccagttg
    22921 cccatgcgtt gggtccccag agagcaaatc gggacgcgcc agctccgcaa gtaacgttgc
    22981 cgtgtcgatc aacgtcccct ccgttgctct tcgcacgccc ggtttaactt ctttgacaaa
    23041 aatagccagt acgaaccagg ccgccaccgc caccagtaaa aaatagccca gcagcaaccg
    23101 catgccgata cgcattacaa gcccctcagg ctatatccca tgccgcgatg agtattaatc
    23161 ggtgaaagat cggggttgat ggcgcgcagc ttggcacgca gcgttttaat gtggatatcg
    23221 acggtgcgat cgtaggtgtc ctgcgcatct tcccacacgc tatccattag ttgctggcgg
    23281 gaccagacgc ggcctggcga cttgagtaac gtcttcagca ataaaaactc ataccgcgtc
    23341 agcgttaatg gcgtgtcaaa ccagctgatc tgcgccgcgg gttcattcaa ttcaaaatga
    23401 ccaatacgga tgacgggaga cggcgtcgag aacttcttca cccgacgcag taaggtgcgc
    23461 accctggcgc acacttcgcg gggtgaaaac ggtttagcca catagtcgtc agcgccaatt
    23521 tccagcccaa gcaggcgatc gacctcttca cttcgggccg tcaggaacag tacaggtaac
    23581 gccggataga gcgccagtaa ctggcggcac aattcaaagc cactgatatc aggcaaacca
    23641 acgtcgagaa tcatgacgtc gggaacctgc tggcgagctt tatccagcac cggcaaacct
    23701 cgctcaaaga cctcgacggc aaaaccttcc tgctgcaaca tgtagaccag cgtgtcggct
    23761 atcccttgct catcttccac taaccagacc gtttcccgtt gcataaatcg cctctgttat
    23821 tgccgccacg gcatgacagg aaccgcgcta atcgcgtttt tcggcgaacc ttctacaact
    23881 ttgtcggagt aagccagata agcgagcgcg ttgcgtttgg cgtcataaaa gcgcacgacc
    23941 tgtaacgact taaagaccag ggacgtgcgt tttttgaata ctacctcgcc ctgagctttg
    24001 ccgtttttaa tacgatccga cagttcaatc ggcccgactt gctgacaaga aatggccgca
    24061 tcggaggtat cttctgccag gcccaatccc cctttaatac caccggtttt cgcccggctc
    24121 acataacagg tgacattttt cacatcggga tcgtcaaagg cttccacaac aattttgtga
    24181 tccgggccga tcattttaaa tacggtgtcg accgaaccaa tctcttctgc atgagccaat
    24241 ggccccagca ttatcaggct taaagacagg atcaaatgct tgtatttcat attgttacca
    24301 tttttatcta ttacacgagc aattagttcg tcacggtaaa taacatattg attaagaaca
    24361 aaatctcaat tcttttattt ccgtcaagcc ctaaaacata ctctactaag aaaaaaacac
    24421 tgaatgctaa aacagcaaaa aatgctatta tccaattacc tgatgtcagg tgctcgttgt
    24481 tgaaaggatg aggatatttt atggatcagg ccggcattat tcgcgacctt ttaatctggc
    24541 tggaaggtca tctggatcag cccctgtcgc tcgacaatgt agcggcgaaa gcaggttatt
    24601 ccaagtggca cttacagaga atgtttaaag atgtcactgg ccatgctatt ggcgcgtata
    24661 ttcgtgctcg tcgtttgtcg aaatcggcgg tcgcactacg cctgactgcg cgtccgattc
    24721 tggacatcgc gctgcaatac cgcttcgact ctcaacagac atttacccgc gcattcaaga
    24781 agcagtttgc ccagactcct gcactttacc gccgttctcc tgaatggagc gcctttggta
    24841 ttcgcccgcc gctacgtctg ggtgaattca caatgccaga gcacaaattt gtcaccctgg
    24901 aagatacgcc gctgattggt gttacccaga gctactcctg ttcgctggag caaatctctg
    24961 atttccgcca tgaaatgcgt tatcagttct ggcacgattt tctcggcaac gcgccgacca
    25021 ttccgccggt gctctacggc ctgaatgaaa cgcgtccgag tcaggataaa gacgacgaac
    25081 aagaggtatt ctataccacc gcgttagccc aggatcaggc agatggctat gtactgacgg
    25141 ggcatccggt gatgctgcag ggcggcgaat atgtgatgtt tacctatgaa ggtctgggaa
    25201 ccggcgtgca ggagtttatc ctgacggtat acggaacgtg catgccaatg ctcaatctga
    25261 cgcgccgtaa aggtcaggat attgagcgat actacccggc agaagacgcc aaagcgggag
    25321 atcgcccaat taatctgcgc tgtgaactgc tgattcctat ccgccgttaa cgctgcagct
    25381 catctaatgc aggggcgtcc agatgcgaga tgtcccctgc ggtttcaacg acccagccag
    25441 acgccagcca caggctttcc tgataatcca cgcgcgaaat agaacagtta cgcagacgta
    25501 agcggcgctc tgcccatgct ggtaatccga gaatcgtact caccagacat cccagtgcaa
    25561 taccatggct caccagcagc ggtcggcttc cctgcggtaa gtcccggcaa gattccagtg
    25621 ccgcattgac gcgatcgctg agctcctgca ttgactcgcc ttcaggaata cgcccgtcaa
    25681 cggtgccatt gaccagctgc cgacgccagt tctcttcttc ttcggtcaga gaatcgatat
    25741 gtcttttttc cagcacaccc atgtttaatt cacgcaggcg agaatcaaag atgatgtcac
    25801 agccgcaggc ctgggcgatg atttccgccg tacgccgggt gcgtcctaaa tcgctactga
    25861 taatatgcgt aatgccaagc tctttggcac gggttgccac ctgcatcgcc tgttgctcac
    25921 ctttggcggt cagcgggctg tcagactggc cctgaatacg tcgctcggcg ttccactgcg
    25981 tttcaccgtg gcggactagg tatacctgta acatgctttt tttccgtata ctgctgtaac
    26041 gttaattttc tgagtaatgc tgattatgca ccaagttgtc tgtgcgacca ccaatcccgc
    26101 taaaattcag gccattctgc aggcatttca cgagatcttc ggcgaaggat cctgccatat
    26161 tgcatccgtt gccgtcgaga gcggtgtacc ggaacaaccc tttggcagtg aggaaacgcg
    26221 cgctggcgca cgaaatcggg tagcaaatgc ccgccgttta cttccagagg ctgatttttg
    26281 ggtggcgatt gaagctggca ttgatggcga cagcactttc agttgggtgg tgattgaaaa
    26341 cgccagccag cgcggcgagg cgcgttcggc aactttgccg ttacctgcgg tgattctgga
    26401 aaaagttcgt gaaggcgagg cgcttggtcc cgtgagtcgc gttataccgg tattgatgag
    26461 attggtcgta aggaaggggc gatcggcgtg tttaccgccg ggaaactcac tcgcgccagc
    26521 gtctatcacc aggcggtgat cctggcgctg agtccgtttc acaatgccgt gtatcaggca
    26581 ttgtaggacg gataaggcgt tcacgccgca tccgacattc ggtgcacgat gcctgatgcg
    26641 acgctgccgc gtcttatcag gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag
    26701 ccactgacgc agctccacgg gcgcggcttt caggctgtta gatccacgcg taatcgtcgc
    26761 gatgcccgcg ccgagttcat tttttaactc acgctgactc atttcgccac gcaacagctc
    26821 ttcgacaata cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt
    26881 taacaacggt aaatggagat cgttttggta ggcattctta agcaggtcga caaaacgtaa
    26941 ccactcctgg tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt gggccataat
    27001 atgtcgccat tgttagcggg ggaagcaaaa tgactccccc ggttgtactc gctaaagagt
    27061 acgatagcat atcataaacg tgcggatcag taacgacgtc cccattccgt ggcgctcatc
    27121 aacgtcggtt tatcccccat gaaatagcgg tagtaagcat cataagccag cacgttcttc
    27181 acataaccgc gcgtctcgga gaacggaata ctctcgacaa atgccacagc gtcgatacgc
    27241 ccggcgctgt tgccaagcca ggttcgcacc cgtccaggtc cggcgttata agctgctgag
    27301 gagaaaatac ggttattgcc aaactgctga taaacatatt gcaggtaact ggtgccaatg
    27361 ttgatgttcg tttccggatc cagcaattgc ccagggctgc tataaccagg aatagagaac
    27421 atcttcaccg tatgggtcgc tgtaccaggc ataatctgca tcaggccgct ggcccctacc
    27481 ggtgatttca ctttcggatt ccaggcgctc tcctgacggg caatcgccat cgcatagctt
    27541 tgcgggatct ctttcccact ggtgtaacgt ttgaaaagat cgttgtaagc cagcgggaat
    27601 cgctcttcca gatgatccca aagcttcccg gcgatcgttg cctgaacgct aagatcccac
    27661 cattggttat tgaaagcata ccgagccagt tgagcctgct ctgtttttga cttgctcttc
    27721 accagattgg cccactcgct acgcgcggta ttatcgagat tccagtacat caactcgcgc
    27781 acgcgcgcca tctccggccc ctgagtcagg gtgctgtcaa cattctgcgg cgctttatca
    27841 atcttcagct catactcttc accgatgcgt tgtgctgcaa ccatcgggta gaaaccacgc
    27901 tgttgtatga gttgatgcaa aatctctttc gcttcagctt cacgtccgcg ttccagcaat
    27961 aaatccgcct gccagtaacg ccattcatct ttctcttttg cttccatcgg cagacgcgcc
    28021 agccaggtat tcaggccgcg acgatcgccg gtgccaagcg ccattcgcac acggcgttca
    28081 atcaacgacg tggattgcga gcgcataatg gcgtcatcgc gccatttcgc ctgctcatcg
    28141 gtgacatcat tgcccatcaa acgccaggcg acgatatcgc gcagctcctg aatctgatct
    28201 tcattaagct gctgcgcctg ggcaagcgac gggatcatca gccgtgcatt ttcggcatcc
    28261 tgccgcgcca cactggcaaa cgccaccgcc gccatttgac gggtaaaatc ggtcgcgccg
    28321 gttgtacgcg cgaaggtcag taccgtatta gggttgttcg ccagtgaaat gattgccgag
    28381 gcgatagtct ggtaatcggc aggcatctgc cctgccagca ctgttaccag gcctgtgtta
    28441 cccgctttca tcgccagacg gatacgctct aaatacgcca gcggatcttg tttacctgac
    28501 gcacgccaga cgctaaataa cttgtcacag gcgttaggct ggctcttgcc ggttagccac
    28561 agctctttcg ccccttgcca ggcttcttca ctctgcccgg tgttccattt cgcatagtag
    28621 taattacatt gcgcttcggt agttccgggc ttttccgggc taaaggctaa caagccacgc
    28681 cagtcttcac gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg agcgggagga
    28741 agcgtggggt tagcgcgaac aaagttagtg accgtcaccg ccggttgatt catcaggtca
    28801 tcggtgatct ggcggtattc caggtaggga taaagcggat aatccttcag tccaggcatc
    28861 atttgttcga ccacatccat ctgtcgatta tcccaggcct gcttgatttg cgcgtaacgg
    28921 ctacgctgct catccagtga gtcggctcgc gccacgctgc tgaccgtcag cagacagaca
    28981 ccggcagcca acagccgcca ggtaacttgt ttggcttttt ccacaagcgc atcctctaag
    29041 tgtaaataca tcaatgcagc gtcatgccgc gtaatagatt tatgctaacc agtcattgcc
    29101 gtttacgcca cgttacggac acttttttac ttttactgcg aaggtgatcg accaactggg
    29161 attaggcggg gaaacaggct acacttcgcc tttgaaaact catcatcacg ataaacatag
    29221 aggcgaagtc caacgtggct caattcgttt ataccatgca tcgtgtcggc aaagttgttc
    29281 cgccgaaacg tcatattttg aaaaacatct ctctgagttt cttccctggg gcaaaaattg
    29341 gtgtcctggg tctgaacggc gcgggtaagt ccaccctgct gcgcattatg gcgggcattg
    29401 ataaagacat cgaaggtgaa gcgcgtccgc agccagacat caagattggt tatctgccgc
    29461 aggaaccgca gctgaacccg gaacacaccg tgcgtgagtc cattgaagaa gcggtttcag
    29521 aagtggttaa cgccctgaaa cgcctggatg aagtgtatgc gctgtacgcc gatccggatg
    29581 ccgattttga caagctggcc gctgaacaag gccgtctgga agagatcatt caggctcacg
    29641 acggtcataa cctgaacgta cagctggagc gtgcggcgga tgcgctgcgt ctgccagatt
    29701 gggacgcgaa aatcgctaac ctctccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc
    29761 tgctgctgga aaaaccagac atgctgctgc tcgacgaacc gaccaaccac ctggatgccg
    29821 aatccgtggc gtggctggaa cgcttcctgc acgacttcga aggcaccgtg gtggcgatta
    29881 ctcacgaccg ttacttcctc gataacgttg caggctggat cctcgaactg gaccgcggtg
    29941 aaggaattcc gtgggaaggt aactactcct cctggctgga gcagaaagat cagcgtctgg
    30001 cgcaggaagc ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg
    30061 tacgtcaggg aactaaaggc cgccagtcga aaggtaaagc acgtctggca cgctttgaag
    30121 agctgaacag caccgaatac cagaaacgta acgaaaccaa cgaactgttt attccacctg
    30181 gaccgcgtct gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc tacggcgatc
    30241 gcctgctgat tgatgacctg agcttctcga tcccgaaagg ggcgatcgtc gggatcatcg
    30301 gtccgaacgg cgcgggtaaa tcgaccctgt tccgtatgat ctccggtcag gaacagccgg
    30361 acagcggcac catcactttg ggtgaaacgg tgaaactggc atcggttgat cagttccgtg
    30421 actcaatgga taacagcaaa accgtttggg aagaagtttc cggcgggctg gatattatga
    30481 agatcggcaa caccgagatg ccaagccgcg cctacgttgg ccgctttaac tttaaagggg
    30541 ttgatcaggg taaacgtgtt ggcgaacttt ccggtggtga gcgcggtcgt ctgcatctgg
    30601 cgaagctgct gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg
    30661 atatcgaaac cctgcgcgcg ctggaaaacg ccctgctgga gttcccgggc tgtgcgatgg
    30721 ttatctcgca cgaccgttgg ttcctcgacc gtatcgccac gcacatcctg gactaccagg
    30781 atgaaggtaa agttgagttc ttcgaaggta actttactga gtacgaagag tacaaaaaac
    30841 gcacgctggg cgcagacgcg ctggagccga agcgtatcaa gtacaagcgt attgcgaagt
    30901 aatgcgtaaa atgccggatg cggcgcgaac gccttatccg gcctacaaaa ccagcaattt
    30961 caatacgttg taaaactgta ggcctgataa gacgcggtaa gcgtcgcatc aggcattgag
    31021 caccgaatgc cggatgcggc gtgaacacct tttccggtct acaacagcct tagcatcctc
    31081 aatttcgtgg cgggtcaccc gtgcaggttt tttcaaacct tgtttaatct cgacagcctc
    31141 ttccagagag gttttcaact catcaaaaaa actcatttta tacctcgcct ttgagcagtt
    31201 tcgtcacggt tatccttgct cccccatcat ctcccgcacc agctccacgc agcgcaggaa
    31261 acggctgtcg taatcgtcct cttcaacccg cacgaattcg atattgttct cttccagcat
    31321 ctccaccagc aagttctgga actctttgcg atccaccgaa ctgccgaggc tgcgtaaacc
    31381 atccgccacc cacggcgtgt tgttctccag caggatcacc agatcgaaac ggtattcatc
    31441 aatcagcgcc tgcacgaacg gatgctcacg cccttcgtac tttttgcaga acgcctgggt
    31501 agtgacaaaa tcggtgtcaa taaacgccac tttattggcg tatttcactg caaaatcaat
    31561 gtattgtgcg tgacccaacg cgattttatc gtagtcggaa tactgcaatg cgatttcatc
    31621 accgccgagg tgtgaaaaga cataatcgcg gccatattcc cacgcactgg tggtgttgaa
    31681 gatattggca agtttgttta ccagggtgga tttaccgctc gactcgccgc caaggatcgc
    31741 cacggtacgc acaaaaaacg gcttcacttc ggtaggaata tattcccagt agcggaacgg
    31801 gttttcgcgg atctgcgcac cgctgatact cataaaggta cgtttcggat cgaccagcac
    31861 cgtctcgatc cccagatgtt ccatatactg tggcgcatcg gcttcttccg aggtgtagat
    31921 caggtccggc tggatcccct tttcagccat aaactttttg atgccgttgc tccacacatc
    31981 ccagccatgc ggatacggct ccatgccctc ttcgttgaaa gcatgaatac ggatattttt
    32041 ctgatattta aaggtttgca atagccagcg cagacgatcc ggcaccgtgg gctgctgcga
    32101 catggcgctg tcttcaaaca acgcgcggtc gcgggtatcg tcaaaaccca taatgatatg
    32161 cagctcgtca acctggctac aggcgcgctg gataaggtag atatgtccgg tatgcagcgg
    32221 gtagaactta ccgaatacga caccaatggt tttcttctgc cggggaaatt caagccccaa
    32281 aaaacggtgc aatgcctcca gcttttgcgc gctggggctt ttaattttgg cattcagtaa
    32341 ctggcttaaa taccctttgg tcataccgct ggcatcagct acctgctgta gcgtgcagcc
    32401 ctgttgcttg atggcagttt tcaggtaatc aaatgacgac atatctccct ccgtatctct
    32461 cattataagt cgtcgaacac gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc
    32521 atcccttccg gcgctttttt cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg
    32581 tgtttcgccg cttcagaaat ccgctcctgg ccactgggca ccgggcggat ctcccctgcc
    32641 agcccgactt caccaaacac caccagatcc tgtggcagcg gtctgtcacg caggctggaa
    32701 accatcgcca gcagtaacgc taagtcggcg ctggtttcgg ttaccttcac gccgccgacc
    32761 acgttcacaa acacatcctg atcggccatt tgcagaccac cgtgacggtg caagacagcc
    32821 agcaggattg ccagacggtt ttgttccagc cccactgcca cgcggcgcgg attcgccatt
    32881 atcgagtgat cgaccagcgc ctgaatctcc accagcaacg gacgcgttcc ttcccacacc
    32941 accatcacgg agctgccgga ggtcacttca tctccgcgac ttaagaaaat tgccgaaggg
    33001 ttgctgactt cacgcagccc ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg
    33061 ccgaagcggt ttttatggct gcgtaaggtg cgaaaacggg agtcggcatc gccatccaga
    33121 agcaccgaac agtcgataca gtgttccagc actttcgggc cagccagcga accatctttg
    33181 gttacgtgtc caaccatgac aatcgccaca ccgcgcgttt tggcgaaacg cgtcagataa
    33241 gccgccgttt cacgcacctg cgccacgcta ccaggcgatg attgtacatc cgccatatgc
    33301 atcacctgga tcgagtcaat taccatcagc ttcggttgct cttcttcggc aatcaggcag
    33361 atctgctcga tgctggtttc cgacaacata ttgagattgt cagtcggcag gccaaggcga
    33421 tgagcgcgca ttgccacctg ttgcagcgac tcttcgccgg tgacatacag cgttttcatc
    33481 tgctgggcca gtttgcacag cgtttgcagc aacagcgtgg atttccccgc accagggtta
    33541 ccgccaatca gaatggcact tcctggcacc acgccgcctc ctagtacgcg gtcgaactct
    33601 ttaaatccgg tggaaaaacg cggcagctct tcaaggctga tatcggagag tttctggact
    33661 tttgccaccc cggcgctacc ggcatagccg ctgagacgct cgttacgcgc caccgttggc
    33721 gacgcagcaa gacgcacctc ggtgatggtg ttccaggcat gacaggcact gcactgcccc
    33781 tgccagcgcg gataatcggc cccgcattca ttacaaacaa aggcgcgttt tggagctttt
    33841 gccacggtgt acctcgttaa tgctgtgccg cccgcaggat gacgggcagg caattacttc
    33901 tgattcaggc tgccagagag gatgcagaat acccccatca ggtcagcgtg acggatggtg
    33961 acttccgcct tttcattcac ttttggcttg gcatggtagg caatccccag ccctgctgct
    34021 ttgatcatcg gcaggtcatt agctccatcg ccaatcgcca cggtctgcgc cagcgggatt
    34081 tcatactcct gcgcgaggcg agtcagagtt ttcgctttgt actgcgcgtc tacgatgtcg
    34141 ccgatcacat tgccggtaaa tttaccgtcc atgatctcca gttcattggc taccacggct
    34201 gtcaggcgca gcttgtcgcg caggtattca gcaaagaaag taaagccgcc ggaggcaatc
    34261 gccactttcc agcccagcgt ttccagcttg agcaccagct gcgttaagcc tggcatcagc
    34321 ggcagatttt cacgcacctg ttgcagaata ttggcgtcag cgcctttcag cgtcgccacg
    34381 cggctgcgca ggctggcggt aaaatcgagt tcgccgcgca tcgcccgttc ggttacctcc
    34441 gccaccatct cgcccgttcc ggccagcttg gcaatttcat caatacattc aatctggatg
    34501 gcggtggaat ccatgtccat caccagcaaa cccggcgtgc gcaggtgcgg gattttcccc
    34561 agcggggcga catccagctg cgcttcgtgg gccaggcgtg tagcccgtgc ggtgagtgaa
    34621 cctgccagac gaatcacctg ataatcttcc acgcaccagg cggcaacaat caccatcgcc
    34681 gcacccagtt tgctctggta ttgggtcaga cgctgtttat ccagcccacg accatacagc
    34741 agccagccgc tacgacctgc gtggtaatcc agtggcatca cttcatcacc acttaatgaa
    34801 agaggcagac ccggccataa agagacatct tcaggcaggt cgcaccaggt aatgttaggc
    34861 attaaggctc ctgtaaaatc gttcgaagca gggaaaataa cgcatgaggc taccttgtat
    34921 ccattgcttc tggcaacatt aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa
    34981 aactgaaatt ccggctacat cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg
    35041 cactgatgca gggggcgtca tggtttagtc aaaaccacca gcgacagcgt aatccacagc
    35101 tggaagaact ggcccgcacc ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc
    35161 gtaccgactc accggatgaa aaacgcattc aggcgatcct cgatcagtta acagatgaaa
    35221 gccgtatcct cgacgcgggt gtgtatgacg aacaaggcga tcttatcgca cgttctggcg
    35281 aaagcgtcga agtgcgcgat cggctggcgc tcgacggtaa aaaagcaggc ggctatttta
    35341 accagcagat tgtcgagcca attgcgggta aaaacggacc gctcggctat ctgcgcctga
    35401 cactcgacac ccatacgctc gccaccgaag cccaacaggt ggataacacc actaacattt
    35461 tacgcctgat gttgctgctc tcactggcaa tcggcgtagt gctgacccgc acgctgctac
    35521 agggtaaacg cacccgctgg cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc
    35581 cggaagagga agaaagcgag aaaaaagagt gacccattac tacaagaaag gaaatcgtta
    35641 tgtccacatt acgcctgctc atctctgact cttacgaccc gtggtttaac ctggcggtgg
    35701 aagagtgtat ttttcgccaa atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg
    35761 ccgacacggt agtaattggt cgcgcgcaga acccgtggaa agagtgtaat acccggcgga
    35821 tggaagaaga taacgtccgc ctggcgcggc gcagtggcgg tggcggcgcg gtgttccacg
    35881 atctcggcaa tacctgcttt acctttatgg ctggcaagcc ggagtacgat aaaactatct
    35941 ccacgtcgat tgtgctcaat gcgctgaacg cgctcggcgt cagcgccgaa gcgtccggac
    36001 gtaacgatct ggtggtgaaa accgtcgaag gcgaccgcaa agtctcaggc tcggcctatc
    36061 gcgaaaccaa agatcgcggc ttccaccacg gcaccttgct actcaatgcc gacctcagcc
    36121 gcctggcaaa ctatctcaat ccggataaaa agaaactggc ggcgaaaggc attacttcag
    36181 tgcgttcccg cgtgaccaac ctcaccgagc tgctgccggg gctcaccatg agcaggtttg
    36241 cgaggccata accgaggcct ttttcgccca ttatggcgag cacgtggaag cggaaatcat
    36301 ctccccggac aaaacgccag acttgccaaa cttcgccgaa acctttgccc gtcagagtag
    36361 ctgagagtgg aacttcggtc aggctccggc attctcgcat ctgctggatg aacgctttag
    36421 ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc catatcaccc gcgcccaggt
    36481 atttaccgac agcctcaacc ccgcgccgct ggaagccctc gccgggcgac tgcaaggctg
    36541 cctgtaccgc gcggatatgc tgcaacaaga gtgcgaagcg ctgttggttg acttcccgga
    36601 ccaggaaaaa gagctacggg agttgtcgac gtggatagcg ggggcggtaa ggtaactcgc
    36661 tgattaaaat atccaaggaa ggaaatatga tcgatctgga aaaccaggaa cgggagatta
    36721 tcaatttaat gttcagccag cgtatttcct ggctagccgc cgtgcgtatc aggcataaat
    36781 tatcgcttgc tgaagtaagt aaaatgttag gtatctcgat taattctcta aaacagatcg
    36841 aaaagacgga acggcttagc tcaaacatca aaagcaaaat ggcagaaatt tatggttgtc
    36901 caccagagtt gctgatatgc ccttcctgga tgacggctga acataaatga atagattcgt
    36961 gaatagatac ttgaatgttt tttattttct atccatatga aaaataatag taaatctata
    37021 aatcagttgc tattgatgaa tagatactaa aaagccggag cagtccccca ctccggctta
    37081 acacgacaat cgcctttcag cgaaacacaa ttactcttta tcgcccagca gaacggattc
    37141 cagtgcgatt ttgatcatgt cgttgaaggt ggtctgacgc tcagcggcag tggtctgctc
    37201 gtgagtgcgg atgtggtcag acacggtgca gatggtcagg gctttcgcgc caaactctgc
    37261 agccacgccg tagataccag ccgcttccat ttccacgccg aggatgccgt atttttccat
    37321 cacgtcgaac atttcgccgt ccggagagta gaacaggtca gcggagaaca ggttacccac
    37381 gcgagcatca acgcccagtg ctttagctgc atctactgcg ttacgcacca tgtcaaagtc
    37441 agcgatagcg gcaaagtcat ggtctttaaa acggatgcgg ttaactttgg aatcggtgca
    37501 ggcccccata ccgataacga cgtcgcgcag ttttacgtgc ggcagaactg cgccacagga
    37561 accaacgcgg ataattttct tcacgccgaa atcggtgatc agttctttgg tgtagatgga
    37621 gcaggacggg atacccatac cgtgacccat tacggaaatt ttgcggcctt tgtaagtacc
    37681 agtgaagccc agcataccgc gaacgttgtt cacttcacgg gcatcttcaa ggaaagtttc
    37741 agcaatatac ttcgcacgca gcgggtcgcc tggcatcaaa actacgtcag cgaaatcgcc
    37801 catttctgca ttaatgtgtg gggtagccat tgttttatcc tttttgtgac ataacaaagg
    37861 cggagtcaac gctccgccca aatccatcag aacatggctt tgccatattc catatcagaa
    37921 gtaccaaaat attttgccag agtctggccg atatccgcga aggtttcacg gtgacccagt
    37981 gagcccggtt ttactttcgg gccatatacc agtaccggaa tgtgttcacg cgtgtggtca
    38041 gtaccggtcc aggtcggatc gcaaccgtgg tcagcggtga ggatcaggat gtcgtcatcg
    38101 cgcagcagag acatcagctc cggcagacgg cggtcgaaca gttccagacc cgcagcataa
    38161 ccggcgacgt cgcggcggtg gccccaggaa gagtcgaagt caacgaagtt ggtgaagacg
    38221 atggtgttat cacccgcttc tttcatctct ttgatggtgg cgtcaaacag cgcgtccaag
    38281 ccagtcgctt tcactttttt ggtgataccg cagttggcgt agatgtctgc aattttaccg
    38341 acagaaacca cctggccgtg tttttcatca accagtttct gcagcacggt cggtgctggc
    38401 ggctcaacag ccaagtcgtg acggttaccg gtacgctgga agttaccagc tttgtcaccg
    38461 ataaacggac gagcgataac acgaccgata ttgtagccgc cgttggtcag ctcttcacgg
    38521 gcgatttcgc acagttcgta gagtttatcc agaccgaaag tttcttcatg gcaggcaatc
    38581 tggaacacgg agtcagcgga ggtatagaaa atcggcttgc cggttttcat gtgctcttcg
    38641 cccagttgat ccagaatgac cgtaccggaa gagtggcagt taccgaggta acccggcaga
    38701 ttagcgcgtt cgaccagttt atccagcagc tcttgcggga agctgttttc gtgatcggag
    38761 aaatatcccc actcaaacag aaccgggaca ccggcgattt cccagtgacc agacggggta
    38821 tctttaccgg atgacatttc gtgcgcccat gcgtacgcgc cgataacttc agcgttgccg
    38881 tccattcccg ccggaatgaa accggtagaa ccttcgtgtg ctttcgccag ccccagacgg
    38941 gtcagatttg gcagattgag cggcccttta cgaccattat cagcttcgcc tttggcacaa
    39001 gcttctgcga tatgacccag ggtgtcagcc ccgacgtcac caaagcgttc tgcatcttct
    39061 gtagcgccga tgccgaatga gtccagcacc ataataaatg cacgtttcat atgttctccg
    39121 tactcagtgc ttcaaattta aatgatcaga tcagtatacc gttattcgct gatacggcga
    39181 tagacagttg gtgtgctttc cggtgcgtta tcggcaagtt taattgccgc tttcaccgct
    39241 ttcgccgctt cctgccagct gttttcgtct ttcgcgagga taaccgccag cggacgctga
    39301 ccgtctacct ggtcgcccag acgcgccata tcagtaaagc cgacgctgta atcgatggtg
    39361 tcagatgcct gacgacgacc gccgcccatt gcaactactg ccatccccag cgcgcgggta
    39421 tccatttcac tgacaaaacc ttcggtatca gcatagactg ctttcgtcag catcgctgtc
    39481 ggcagatact tcgcgtagtt ctcaacgaag tcggtcgggc ctttttgtgc cgctaccata
    39541 cgaccaaaga cttctgccgc tttaccgttg tccagcaccg cctgcaattt cgcgcgcgct
    39601 tcggcgtcat ctttcgccag tttgccggag atcaacatct ccacgcacag cgccatcgtg
    39661 acatcaaaca gacgcgggtt acgatactca cccgtcagga actgcaccgc ttcacgaact
    39721 tcaaccgcgt tacctgcact ggaggccagt acctgattca tgtcggtgag cagcgcggtg
    39781 gtgcgcacgc cagcgccgtt agccacgcca acaatcgctt cggcaagggc ttcagagagt
    39841 tcgtaggtcg gcataaacgc gccgctaccc actttcacgt ccatcaccag cgcgtccaga
    39901 ccttccgcca gtttcttcgc caggatcgag gcagtgatca gcgggatgga gtccacggtt
    39961 gcggtaatat cacgggtcgc gtagaaacgt ttatcagccg gagccagtga actggtctga
    40021 ccgataatcg ccacgccgac gtctttaata atttcgcgga aacggttgtc atccgggaaa
    40081 atgtcgaagc cagggatgga ttccagtttg tcgagcgtac cgccagtatg accgaggccg
    40141 cgaccggaga tcatcggaat atagccgccg caggctgcga ccatcggccc caacatcagc
    40201 gaagtgacat cgccgacgcc gccggtcgag tgtttatcaa caatcgggcc attcagatgc
    40261 aggcttttcc agtcgagaac ggttcctgaa tctcgcatcg ccatggtcag cgagacacgc
    40321 tcaggcattg tcatatcgtg gaagaaaatg gtcatcgcga gggcggcaat ctgcccttcg
    40381 gaaatagtgt tgtcgcgaat accgttgata aagaaacgaa tttcttcatc gctcagcgca
    40441 tgaccatcac gttttttacg aataatttct tgtgcgagaa acaaggtaac ctcccgagga
    40501 aaagagtatg aggcggcttt tgccggatgc gtctacgcct tatccggcct acgggtagat
    40561 acgtctcgta ggcctgataa gcgcagcgca tcaggcgtaa agcatcttac ttagtagctg
    40621 ctggcgctct taccgtcgcc gtggcccagc gctttcagca ggcttgccag caggctggaa
    40681 gcaccaaagc ggtagtgacg cgcatcagcc cagtcagcac cgaacagttc atctgcgatg
    40741 gcgagatatt tctgcgcatc ttccgcagta cgcacgccgc ccgccggttt gaaaccaacg
    40801 gttttttcta cgcccatatc acggatcact tccatcatga tgcgagcgct ttccggcgtc
    40861 gcgttcacag ccactttacc ggtagaggtt ttgatgaagt ccgcacccgc tttgatggag
    40921 atttcagacg ctttacggat cagcgcttcg tctttcagtt cgccggtttc gatgatcact
    40981 ttcagcagta cattcgctgc cgcgcaagcc tctttacagg ctttcaccag gtcaaaacca
    41041 acctgctcgt cacccgccat cagcgcgcgg tacgggaaca ccacgtcaac ttcatcagca
    41101 ccgtaggcga ttgccgcacg ggtttctgcc agcgcgattt cgatgtcgtc gttaccgtgt
    41161 gggaagttgg ttaccgtagc gatacggatt tccggggtgc cctgctcttt cagagttttg
    41221 cgagcaatcg ggataaaacg aggatagata cagatagcgg cggtattgcc gaccggagtt
    41281 ttggcctgat gacacagagc aattactttc tcgtcggtgt cgtcgtcatt cagggtggtc
    41341 aggtccatca atttcagtgc acgcaggctg cttgctttca gatcagtcat ttcattctcc
    41401 tggcttgtcg ccaataaaat tcaccttgcg agtttgttag tattctaaca tctactccgc
    41461 aacacacttc gatacacatc acaattaagg aaatctactt acaagtttgc atcactgtaa
    41521 tgcgatctgg ttcaaataat tcactttcaa atgaatgcgt cagtggtggc aaacgcatca
    41581 ggatcaaagt gaacatcacg aaacttctta caatggcgca tcaccttcgg cataaaagga
    41641 aaagaagatg cccacttctc atgaaaatgc actgcaacaa cgttgccagc aaattgtcac
    41701 cagcccagtg cttagcccgg agcagaagcg ccattttctg gcactggaag cagaaaacaa
    41761 tctgccttac ccacagcttc ctgccgaagc ccgccgcgcg ctggatgaag gtgtaatctg
    41821 cgatatgttt gaaggtcatg cgccgtacaa accgcgctat gtcttacccg attacgctcg
    41881 ttttctggcg aacggttccg aatggctgga gctggaaggc gcgaaagatc ttgatgacgc
    41941 actctctctg ctgaccattc tttaccacca cgtaccgtcg gtcacatcga tgccggtcta
    42001 cctggggcaa ctggatgcgt tgttgcaacc gtatgttaga attctaacac aaaacgagat
    42061 cgatgttcga ataaaacgtt tctggcgtta cctcgacaga accctgccag atgcctttat
    42121 gcacgccaat atcggcccgt ctgattcgcc cattacccgc gcgatcttac gtgcagacgc
    42181 gcaggtttca ccgaacctga cctttatcta cgatcctgaa atcacccctg atgacctgct
    42241 gctggaagtg gcgaagaaca tctgtgaatg tagcaaaccg cacatcgcca acggtccggt
    42301 gcatgataaa attttcacaa aagggggcta cgggattgtg agctgttaca actcactgcc
    42361 gctggcgggt ggtggcagca cgctggtacg ccttaacctg aaagccattg ccgagcgcag
    42421 cgaatcgctg gatgacttct ttacgcgcac tctaccgcac tactgccagc agcagatcgc
    42481 catcatcgat gcgcggtgtg aattcctcta tcaacaatca cacttctttg agaatagctt
    42541 cctggtgaaa gaagggctga ttaaccctga acgttttgtg ccaatgtttg gcatgtatgg
    42601 gctggcggaa gcggttaact tgctgtgtga gaaagaaggg attgccgcgc gctacggtaa
    42661 agaagccgcc gcaaatgaag tgggttatcg catcagcgcg caactggcgg agtttgtcgc
    42721 caataccccc gtgaaatatg gctggcaaaa acgcgccatg ttacacgcac agtcggggat
    42781 cagttccgat atcggcacca cgccgggcgc gcgtttgccg tatggcgatg agccagatcc
    42841 gatcacccat ctgcaaactg tcgccccgca tcatgcttat tattattccg gcatcagcga
    42901 cattctgacg ctcgacgaaa ccatcaaacg taacccgcag gcactggtac agctttgcct
    42961 cggtgccttt aaagccggaa tgcgtgaatt taccgccaat gtcagcggta acgatctggt
    43021 tcgcgttacc ggttatatgg tgcgtttgtc ggatttagaa aaatatcgcg ccgaaggttc
    43081 acgcaccaac accacctggc tgggcgaaga agccgcacgc aacactcgta ttctggaacg
    43141 ccagccgcgc gtgataagcc atgaacagca gatgcgcttt agtcagtaag attatcccct
    43201 tctcctgcgt tgacgggcca ggcagtcgtc tggctctgtt tttgcagggc tgcaatctgc
    43261 gctgcaaaaa ctgtcacaat ccgtggacga tgggacgttg caatgactgt ggggagtgcg
    43321 tgccacagtg tccgcatcag gcgttgcaga ttgttgacgg caaagtggtg tggaacgctg
    43381 tggtctgcga gcaatgtgat acctgcctga agatgtgtcc gcaacatgcc acaccaatgg
    43441 cgcaatccat gagcgtggac gaagtgctta gccatgtgcg caaagcggtg ctgtttattg
    43501 aagggataac ggtaagcggc ggcgaagcca cgacccaact gccgtttgta gtggcgctgt
    43561 ttactgctat caaaaacgat ccgcaactgc gccatctcac ctgtctggtg gacagtaacg
    43621 gtatgttgag cgaaaccggc tgggaaaaat tactcccggt gtgtgacggc gcaatgctcg
    43681 atctcaaagc atggggtagc gaatgtcatc aacaattaac cggacgcgat aatcaacaga
    43741 ttaagcgcag catctatttg ctggcagagc gcggcaagct ggcggaactg cgtttgctga
    43801 tgatcccagg ccaggtggat tatttgcaac acatcgaaga actggcggcg tttatcaagg
    43861 gacttggtga tgttccggtt cgcctgaacg cgtttcatgc ccacggcgtg tatggcgagg
    43921 cgcaaagctg ggcgagcgcc acgccggaag acgttgagcc gttggctgat gcattaaagg
    43981 tgcgcggggt gagccggttg atatttccgg cgctctattt gtgacatgcc ggatacggcc
    44041 tacggttcgg cacagacttg taggcctgat aagacgcgtc aagcgtcgca tcaggcattg
    44101 tgcgccaact gccggatgcg gcgtgaacgc cttatccggc ctacggttcg gcacaaacct
    44161 gtaggcatga taagacgcgt taagcgtcgc atcaggcatt atgcgccgac tgccggatgc
    44221 tgcctacgaa gggcaaacta cccacgaacg ttaaacaccg catacgtgtt attaagcaac
    44281 acttcggcaa tctcatccgc gggttccggg cgtaactcgc aaagcacggc aaacacacgg
    44341 gcagcctgtt ccgggcggtt aggctgcccc tgaaaaccgt tgagcggcat atccggcgca
    44401 tcggtttcca gcaacaacga tgccagcggt aattttgcga tgacatcgcg ggttttactg
    44461 gcgcgtggat aggtgatagt accgcctacg ccgattttgt agcccagctg tacaaaccgt
    44521 tcagcctgtt gctggctgcc agaaaatccg tggacaacac cagcacgcgg caaatcgtgg
    44581 cgtttaagat gcatcgccag tttgtcgtgc gtgcgccgtg aatgcaggat caccggcaga
    44641 tcgtagcgtt tcgccagttt cagttgttcg tcgagtaacc actgctgcct ctcaaattgc
    44701 ggatcgtcgc cgaagagatc cagaccgatc tcccccaccg ccaccacctt cgccggacgc
    44761 ctttccagcg cctgctgtag ctgctcaaga gacacatcgc tatgtttttc caacatacca
    44821 ggatgcaagc ccaatacggc atacagcggt tgataatttt ccgctaacgc ctgcacacgg
    44881 gcaaaatttt ccgcctctgt cgccggaaca atgatcttgc ctacgcccgc ttgtgccgcg
    44941 cgttgcaggc tggcctcttc atcgccaata aaaggcggga aatcaaaatg gcagtgagtg
    45001 tcgataaaac ggcaaatcaa gccagatcct cattgttaaa tgtcgggtcg ttcgcctgcg
    45061 gagcgtcgct cacttcggca accagcgtat cgttggcgac tggcgcaggc ggaatgacaa
    45121 tcgattccgg cgtcaccact ggcaccagat ggcgggtaag cggcgctttt tcagttaata
    45181 gcttgccaac cgtggcgagg aaataacgac cgcataaacg cccaagttta tagtcttcac
    45241 gcagcgccgg aatccgactg ccaagcgcga tactatgtaa tggcttcggc ggataaattt
    45301 cggatatccg cagcttgccc ggtggtttct caataaattg ctgaatgtca cgatagctgg
    45361 tttcatgatg ctgcaccaga ttgaccagcg gctgcaggct actgtcaccc agccagcgtt
    45421 ccatgcgttt gaaccactgc ggcgtgtagt acatttgtga cggcacagtg cggatgacga
    45481 ccaacgtttt agcgccctgc cttgccgcct ctttaaccgg aatcgcatca ctgatcccgc
    45541 catccaggta gttaatgcct tccagcgaca ctccgctacg ataaaagcca ggtatcgcac
    45601 tggaggcgcg aatcacatcc agccagtttt gtttggttgg taaaaagtaa ttcggcgcgt
    45661 agtcatcctg acgacaggcg cacatataaa acgatttgcc gctgtcaaac aaccgcgcgg
    45721 cggtatccat ttgcaacggc atctggcttg cggtggcctc caccagccag tcgagatcga
    45781 taagatttcc tccacggaca aagcgcaatg gatcgaaaaa ttcgcgtttt gtggtatagc
    45841 gcatgatgac tttgcgcgcg taaccgggct gattgcagat aaacgccgag aggttctgcg
    45901 ccccggcaga tgtgccgaga taaagatcga aaggattaaa ctgcgcgcgc ataaactcat
    45961 ccagcacgcc agccgtgaaa attccacgct gtccgccgcc ttcgcacacc agagctattc
    46021 gtccaggctg gaacggcctt agcgacaacg gcgcaatatt accaagcgtt acaggtattc
    46081 gctgccccac ttctgccttc ctgtttttgt tgtgaaagaa agacaaagta acgcaattta
    46141 ttattcgcta aaaccgcaaa agccagtccg ctggactggc tttggataaa attttcttaa
    46201 tggtctgaaa tctagggtcg ttttcggccc atgaacaaac tcactaggaa cagaataatc
    46261 ccgacgacaa agacaatttt agctgcgcct gcagcggtac cggccagacc accaaaccca
    46321 agtgcggcgg cgattaacgc gataaccaga aatatgatgc cccaacgaaa cataagcgtc
    46381 tcctttacca tagttaatgt caccgcttga tatgagcgtg aaaatcactc aatgcgatat
    46441 ttttagtgtg gtgcacatcg cgcctcccga caaagttcgg gaggacgaat tacttagttt
    46501 tcagatcatt tttaacgctt ttcacaccat ctaccgcttt ggcgatactt tcagcacggt
    46561 cactttgtgc ctgagaatct acggtaccgg agagctgaac cacgccatcg gtggtttcaa
    46621 ctttcacttt acgtgaaggg acgatatcgt ccgccagcag tttggctttg atttcactgg
    46681 tggtggcggt gtcacccgcg tagcccttca ccgagccttc tttagcgtcg cgaacgtgca
    46741 gtttgtcgct gacagaggtc acgccttcaa cgcctttcgc cactttcact gcctcttcgg
    46801 cctgggcctg actttcaacg aaaccgctca gggtcacgac tttttgatcg gttttgacag
    46861 agatatcggt gctcttgatg ttgtcatgat ccaccagcgc cgccttcact ttcgcggtga
    46921 tggcgctgtc atccatgaaa ttaccgactt tattcataga gctatcgact ttttgccctg
    46981 cgctttcatt ggtagtctgc gcgttgtttt ccgcgtaggc agagccggtc gcgacggcag
    47041 aggtcaacat tacagccagc agagttttcg aaatcttcag tcttgtcata gtcatcgatt
    47101 tattcctgta tgtttgctcg taatttgagc ctagcaacac gaggttgcat tgctgaatag
    47161 ggagagactt caccctctac agaagtcaat ggtcgccatc acaaaagcga tgggtgatga
    47221 ataacgacca ttacagcctc tgaatcagtt attaatatcg gtagaatgac aatcgacggc
    47281 tttagatact aatatctacg cattgaacgg tatttaacgc catcagaaat gtcatcactt
    47341 tgttaaatat agatcacaat tttgaaaccg ctcgggatat cagcgaaaac ataagcaaaa
    47401 gtgaatgttt taagaacatt ccgtaagcgg ctaataagga agggaaattg acagggcgca
    47461 gcggctaccg cgccctggag gcaagaatta atgctcgcgg gtctggtgga actgaacgtc
    47521 cggataacgt tcctgtgcca ggcgcaggtt gaccatgctg gtagcgatgt aagcgaggtt
    47581 atcgccgcca tcaagcgcca gttggctttc gttcttacgc ttgaactctt cgaatttctt
    47641 cgcgtctgca cattctaccc agcgggcagt ggcaacgttg actgactcat acactgcttc
    47701 aacgttgtat tcgctcttca ggcgcgctac caccacatca aactgcagca caccaaccgc
    47761 accaacgatc agatcgttgt tggagattgg acggaacacc tgcaccgcgc cctcttcgga
    47821 aagctgtacc agccctttga gcagctgttt ttgcttcagc ggatctttca ggcggatacg
    47881 acggaacagt tctggtgcga agttcggaat accggtgaac ttcatcatct caccctgggt
    47941 aaaggtgtcg ccgatctgaa tggtgccgtg gttgtgcagg ccgaggatat cgcccggata
    48001 cgcttcttca acgtgcgaac ggtcacccgc cataaaggtc agcgcgtcgg agatcaccac
    48061 atctttcgca gtgcgcacct ggcgcagttt catgcctttt tcatatttac cggacaccac
    48121 acgcataaac gccacgcggt cgcggtgttt cggatccatg ttggcctgaa ttttaaatac
    48181 gaagccggta aatttatctt cgctcgcttc tacggtacgg gtatcggtct gacgcggcat
    48241 cggcgcaggc gcccactcca ccaggccatt cagcatatga tcaacgccga agttacccag
    48301 cgcagtaccg aagaataccg gagtgatttc gcccgcaagg aacagctctt tgtcgaactc
    48361 gttagacgcg cctttaacca gttccagttc gtcacgcagc tgctgtgcca gatcttcacc
    48421 aaccgcagca tcgagatccg ggttattcag ccctttaaca atgcggactt cctgaatggt
    48481 gtgcccttta ccgctctgat agagataggt ttcatcttta taaaggtggt aaacgccttt
    48541 aaacagcttg ccgcagccaa ttggccaagt gatcggtgcg cagccaattt tcagctcgtt
    48601 ctcaacttca tcgagcagtt ccatcgggtc gcggatatca cggtcaagtt tgttcataaa
    48661 ggtgaggatc ggcgtgtcgc gcagacgggt aacttccatc agcttacggg tacgatcttc
    48721 aacacctttt gcggcgtcga taaccatcag gcagcagtcc accgccgtca gggtacgata
    48781 ggtatcttcc gagaagtctt cgtgccccgg ggtgtcgagc aggttaacca ggcaatcgtg
    48841 atacggaaac tgcatcacag acgtagtaat ggagatccca cgctgctttt ccatctccat
    48901 ccagtccgac ttagcgtgct ggttggaacc acggcctttt actgtaccgg cggtctgaat
    48961 ggcctgtccg aacagcagca ccttctcggt gatggtagtt ttaccggcgt ccgggtgaga
    49021 aataatggca aaagtgcggc gcttcgccac ctctttcaaa taaggagaca acgtcataat
    49081 tctttcttct tgagtaagcg gcatcgcgcc gcgcttgttg aaaacgaaaa attgcggcta
    49141 ttttacccat caacgggggg gaggcaatca gtgtttacac aggagctgct ccagttcgtg
    49201 caacgaagaa acggtccagg tgggcgcgat gccttctggt tgctcgcgat tgtgcgcatt
    49261 cagccagcag gtcgcaagcc cggcgttgat gccaccgaga atatcggact cggcagtgtc
    49321 gccaaccatc agcacgcgtg aacggtcagg attgcccgcc tgttccagcg cataatcgaa
    49381 aattttctta ttcggtttgg caacgccaac ttcttcggaa atcaccagca aatcgaagta
    49441 atcacgcagg cccgtccgtt ccagacgcac ttgctgcaag gcgctaaaac cgttggtgat
    49501 gatgccgatt ttagcgttgc tacgaatggc gttaagcaga gaaaccgcgc ccggcaacgg
    49561 cgtacagatt tccgccatcg cattaataaa ggcatcgtta agcaagcctg cttcaacttt
    49621 cagccgttcg gcccagctct cgaaacgccc gtgctgaagc tgtaatgaag tgatcgcgcc
    49681 gttttgataa tccacccaca gtggcttgtt aacggcctga tagtcctgaa aatcttcagc
    49741 ggtaaaggtg acgctgtaat caagaaacat ccgctgcagg ccggtgaatg agtcaaaggt
    49801 aaacagcgtt tcatcggcat caaagaaaat ccagtcccac ttcattattc caccttgtct
    49861 tacatactga ttggcaacgc catgatgatg gcgtcttcgc gaccgtccgt ggtggggtag
    49921 taattgcggc gaatcgtcgc ctcgttaaag cctaaacttt cgtacagggc aatggcggca
    49981 gcatttgagg cacggacttc cagccatagt gtcgccacgc cgcgtttttc cagttcgtcg
    50041 atcagatgtt ccagcagcgc cctacccagc ccctgacgct gatagtcggg atcgaccgca
    50101 atattgaaca atgtagcttc atccagcacc acttgcgtaa tcgcaaacgc cgccattttg
    50161 ccgttttgcg ttaactgaaa gttgagataa cgctcgccct ggttgctggc aaaggttttt
    50221 tcactccacg gaaaggcgtg ggcgcgttgt tcaatgtggt aagccgctgg taaatcagtc
    50281 gtttcgaggg aagaaatcgt gttcatatgt gcaaatttgt tgccataacg cggcgcgtgc
    50341 cgttgggttt gcccgtaatt cggtgagcgc cggtgatgcc acctgagcgc cttccaatga
    50401 tagcggttcg tcagtaccca accgccagct gttgcagcga ctgccttgcg gcagcatcgc
    50461 gattttttct ggcgtcagtt gcagcacttg atcggggctg acggttaatg cgcgcagaac
    50521 atcgctcact aaaggatcag tcagggcggg aagatcgttt gccaccatca ccagacggac
    50581 gtgtgccggg ctcgcaatgg caatctcccc ctgcaacgcg ccagggcgac gcagcgacca
    50641 ctgggtaatg cccagttgct gtaactgcca gtctcgtcgg gatgtcatag cgaatcgctc
    50701 ctgttgtcag gggcgcaaat atagcaaatt cgtcgatacc gcgccaacat atggctataa
    50761 tcgccgccag tatcaattga ggagcattcc atgtctgcat ttaccccggc aagtgaagtc
    50821 ttgctgcgtc acagtgatga tttcgaacaa agccgtattc tgtttgccgg agacttacag
    50881 gatgacctgc ccgcgcgttt agataccgcg gccagccgtg ctcataccca gcaattccac
    50941 cattggcagg tgttaagctg ccagatgggg gataacgccc gttttagtct ggtcgccacg
    51001 gcgaatgatg tcgcagattg cgatacgctg atttactact ggccgaagaa caaaccggaa
    51061 gcccagttcc agttgatgaa tttactttct ctgctgccag tggggacaga tatttttgtc
    51121 gttggcgaga accgcagcgg cgtgcgcagc gctgagcaga tgctggcaga ttatgcgccg
    51181 ttgaataaag tcgacagcgc tcgtcgctgt ggcctctatt ttggtcgtct ggaaaaacag
    51241 ccagtatttg atgcggataa attctggggc gaatacagcg tcgatggcct gacggtcaaa
    51301 acgctgcctg gcgtgtttag ccgcgacggt ctggatgtcg gtagccagtt gctgctctcg
    51361 acgttaactc cgcacacgaa aggtaaagtg ctggatgtcg gctgtggcgc gggtgtactt
    51421 tcagttgcct ttgcgcgcca ttcgccgaaa attcgtctca ccttgtgcga tgtctctgcc
    51481 ccggcggtag aagccagccg cgcaacactt gcagccaacg gtgttgaagg tgaagtcttt
    51541 gccagcaacg tcttttccga ggtgaaaggt tgttttgata tgatcatctc caacccgccg
    51601 ttccacgatg ggatgcaaac cagcctggat gcggcgcaaa cgctgattcg tggcgcggtg
    51661 cgtcatctta atagcggcgg cgagctgcga attgtagcga acgccttcct gccttacccg
    51721 gacgtgctgg atgagacatt tggcttccat gaagttatcg cgcaaaccgg gcgcttcaag
    51781 gtatatcgcg ccattatgac ccgccaggcg aagaaaggtt gagtatctga tatctgtcgc
    51841 cgcataccgt gtcggatgcg gcgtgaacgc cttatccgac ctacaaaacc tgcacgttag
    51901 cccttcgtag gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct
    51961 accaaacctg cacgctagtt tcctgatgga catttttcca gcaattacac atcaggcgat
    52021 aattaactat tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt
    52081 tttaagaatt gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt
    52141 agtgtcctta cg
//
LOCUS       DAJTYM010000017        48384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTYM010000017 DAJTYM010000000
VERSION     DAJTYM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1148
                     /locus_tag="OO575_001016"
     CDS             <1..1148
                     /locus_tag="OO575_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6664070.1"
                     /translation="AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKG
                     QRFVLQPAMGLPSGYSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAE
                     PMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSR
                     VVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIF
                     VYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKVPFNFYNVHYNSTHVVGTSGGSTD
                     DMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIA
                     DFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            1145..2110
                     /gene="yggF"
                     /locus_tag="OO575_001017"
     CDS             1145..2110
                     /gene="yggF"
                     /locus_tag="OO575_001017"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6664071.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2132..2641
                     /gene="fumE"
                     /locus_tag="OO575_001018"
     CDS             2132..2641
                     /gene="fumE"
                     /locus_tag="OO575_001018"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6664072.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2638..3351
                     /locus_tag="OO575_001019"
     CDS             2638..3351
                     /locus_tag="OO575_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6664073.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3323..4000)
                     /locus_tag="OO575_001020"
     CDS             complement(3323..4000)
                     /locus_tag="OO575_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6664074.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(3994..4671)
                     /locus_tag="OO575_001021"
     CDS             complement(3994..4671)
                     /locus_tag="OO575_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6664075.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4659..5042)
                     /locus_tag="OO575_001022"
     CDS             complement(4659..5042)
                     /locus_tag="OO575_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6664076.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5058..5366)
                     /locus_tag="OO575_001023"
     CDS             complement(5058..5366)
                     /locus_tag="OO575_001023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664077.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5367..5945)
                     /locus_tag="OO575_001024"
     CDS             complement(5367..5945)
                     /locus_tag="OO575_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6664078.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5968..6399)
                     /locus_tag="OO575_001025"
                     /pseudo
     CDS             complement(5968..6399)
                     /locus_tag="OO575_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6771..7790
                     /gene="epd"
                     /locus_tag="OO575_001026"
     CDS             6771..7790
                     /gene="epd"
                     /locus_tag="OO575_001026"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6664079.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7840..9003
                     /gene="pgk"
                     /locus_tag="OO575_001027"
     CDS             7840..9003
                     /gene="pgk"
                     /locus_tag="OO575_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6664080.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9218..10297
                     /gene="fbaA"
                     /locus_tag="OO575_001028"
     CDS             9218..10297
                     /gene="fbaA"
                     /locus_tag="OO575_001028"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6664081.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10488..11348
                     /gene="mscS"
                     /locus_tag="OO575_001029"
     CDS             10488..11348
                     /gene="mscS"
                     /locus_tag="OO575_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6664082.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11487..12122
                     /gene="argO"
                     /locus_tag="OO575_001030"
     CDS             11487..12122
                     /gene="argO"
                     /locus_tag="OO575_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6664083.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12215..12955
                     /locus_tag="OO575_001031"
     CDS             12215..12955
                     /locus_tag="OO575_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6664084.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13122..14072
                     /locus_tag="OO575_001032"
     CDS             13122..14072
                     /locus_tag="OO575_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6664085.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14014..15492)
                     /gene="scpC"
                     /locus_tag="OO575_001033"
                     /pseudo
     CDS             complement(14014..15492)
                     /gene="scpC"
                     /locus_tag="OO575_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15516..16301)
                     /gene="scpB"
                     /locus_tag="OO575_001034"
     CDS             complement(15516..16301)
                     /gene="scpB"
                     /locus_tag="OO575_001034"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6664086.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16312..17307)
                     /gene="meaB"
                     /locus_tag="OO575_001035"
     CDS             complement(16312..17307)
                     /gene="meaB"
                     /locus_tag="OO575_001035"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6664087.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17300..19444)
                     /gene="scpA"
                     /locus_tag="OO575_001036"
     CDS             complement(17300..19444)
                     /gene="scpA"
                     /locus_tag="OO575_001036"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6664088.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19648..20541)
                     /gene="argP"
                     /locus_tag="OO575_001037"
     CDS             complement(19648..20541)
                     /gene="argP"
                     /locus_tag="OO575_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6664089.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20924..21583
                     /gene="rpiA"
                     /locus_tag="OO575_001038"
     CDS             20924..21583
                     /gene="rpiA"
                     /locus_tag="OO575_001038"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6664090.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21839..23071
                     /gene="serA"
                     /locus_tag="OO575_001039"
     CDS             21839..23071
                     /gene="serA"
                     /locus_tag="OO575_001039"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6664091.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23300..23359
                     /gene="ibsC"
                     /locus_tag="OO575_001040"
     CDS             23300..23359
                     /gene="ibsC"
                     /locus_tag="OO575_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6664092.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23692..23751
                     /locus_tag="OO575_001041"
     CDS             23692..23751
                     /locus_tag="OO575_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6664093.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23852..24454)
                     /locus_tag="OO575_001042"
     CDS             complement(23852..24454)
                     /locus_tag="OO575_001042"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6664094.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24475..24658)
                     /gene="ssrS"
                     /locus_tag="OO575_001043"
     ncRNA           complement(24475..24658)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO575_001043"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24700..25029)
                     /gene="zapA"
                     /locus_tag="OO575_001044"
     CDS             complement(24700..25029)
                     /gene="zapA"
                     /locus_tag="OO575_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6664095.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25197..25775
                     /gene="ygfB"
                     /locus_tag="OO575_001045"
     CDS             25197..25775
                     /gene="ygfB"
                     /locus_tag="OO575_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6664096.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25801..27126
                     /gene="pepP"
                     /locus_tag="OO575_001046"
     CDS             25801..27126
                     /gene="pepP"
                     /locus_tag="OO575_001046"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6664097.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27123..28301
                     /gene="ubiH"
                     /locus_tag="OO575_001047"
                     /gene_synonym="visB"
     CDS             27123..28301
                     /gene="ubiH"
                     /locus_tag="OO575_001047"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6664098.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATVPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28324..29526
                     /gene="ubiI"
                     /locus_tag="OO575_001048"
                     /gene_synonym="visC"
     CDS             28324..29526
                     /gene="ubiI"
                     /locus_tag="OO575_001048"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6664099.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29600..29683)
                     /locus_tag="OO575_001049"
     CDS             complement(29600..29683)
                     /locus_tag="OO575_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664100.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29974..31068
                     /gene="gcvT"
                     /locus_tag="OO575_001050"
     CDS             29974..31068
                     /gene="gcvT"
                     /locus_tag="OO575_001050"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6664101.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31092..31481
                     /gene="gcvH"
                     /locus_tag="OO575_001051"
     CDS             31092..31481
                     /gene="gcvH"
                     /locus_tag="OO575_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6664102.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31600..34473
                     /gene="gcvP"
                     /locus_tag="OO575_001052"
     CDS             31600..34473
                     /gene="gcvP"
                     /locus_tag="OO575_001052"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6664103.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34740..35483
                     /locus_tag="OO575_001053"
     CDS             34740..35483
                     /locus_tag="OO575_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6664104.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35540..36973)
                     /gene="bglA"
                     /locus_tag="OO575_001054"
     CDS             complement(35540..36973)
                     /gene="bglA"
                     /locus_tag="OO575_001054"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6664105.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37018..37328
                     /locus_tag="OO575_001055"
                     /pseudo
     CDS             37018..37328
                     /locus_tag="OO575_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37492..38151
                     /locus_tag="OO575_001056"
     CDS             37492..38151
                     /locus_tag="OO575_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6664106.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38347..39327)
                     /gene="ygfZ"
                     /locus_tag="OO575_001057"
     CDS             complement(38347..39327)
                     /gene="ygfZ"
                     /locus_tag="OO575_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6664107.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39570..39836
                     /gene="sdhE"
                     /locus_tag="OO575_001058"
     CDS             39570..39836
                     /gene="sdhE"
                     /locus_tag="OO575_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6664108.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39817..40224
                     /locus_tag="OO575_001059"
     CDS             39817..40224
                     /locus_tag="OO575_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6664109.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40264..40785)
                     /gene="fldB"
                     /locus_tag="OO575_001060"
     CDS             complement(40264..40785)
                     /gene="fldB"
                     /locus_tag="OO575_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6664110.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40897..41793
                     /gene="xerD"
                     /locus_tag="OO575_001061"
     CDS             40897..41793
                     /gene="xerD"
                     /locus_tag="OO575_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6664111.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41818..42528
                     /gene="dsbC"
                     /locus_tag="OO575_001062"
     CDS             41818..42528
                     /gene="dsbC"
                     /locus_tag="OO575_001062"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6664112.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42534..44267
                     /gene="recJ"
                     /locus_tag="OO575_001063"
     CDS             42534..44267
                     /gene="recJ"
                     /locus_tag="OO575_001063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6664113.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44358..45456
                     /gene="prfB"
                     /locus_tag="OO575_001064"
     CDS             join(44358..44432,44434..45456)
                     /gene="prfB"
                     /locus_tag="OO575_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6664114.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45466..46983
                     /gene="lysS"
                     /locus_tag="OO575_001065"
     CDS             45466..46983
                     /gene="lysS"
                     /locus_tag="OO575_001065"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6664115.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47026..47574)
                     /gene="idi"
                     /locus_tag="OO575_001066"
     CDS             complement(47026..47574)
                     /gene="idi"
                     /locus_tag="OO575_001066"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6664116.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47629..47700
                     /locus_tag="OO575_001067"
     CDS             47629..47700
                     /locus_tag="OO575_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664117.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47697..47822)
                     /locus_tag="OO575_001068"
     CDS             complement(47697..47822)
                     /locus_tag="OO575_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664118.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47824..>48384)
                     /gene="uacT"
                     /locus_tag="OO575_001069"
     CDS             complement(47824..>48384)
                     /gene="uacT"
                     /locus_tag="OO575_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6664119.1"
                     /translation="VDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVCISSGII
                     LILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYNLYIVAI
                     SLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHADLVKESV
                     SDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11590 a        12131 c        13078 g        11585 t
ORIGIN      
        1 aagcggcgtt actcggtagt gaacataaac gcgtacccga cgatttagaa aatcatccgg
       61 tcattaccgg gcatgaatgt gccggggtta ttgtcgaagt gggtaaaaat ctcactggca
      121 aatataaaaa aggccagcgt tttgtattgc aaccggcgat ggggttacca agcggatatt
      181 cagcgggcta cagctacgaa tattttggcg gcaatgccac ttatatgatt attcccgaaa
      241 tcgccattaa tttgggctgc gtattaccgt atcacggctc ttattttgct gcggcgtcgc
      301 tggcagagcc tatgtgctgc attattggtg cttatcatgc caattatcac accacgcaat
      361 atgtttatga gcatcgcatg ggcgtcaaac ctggcggcaa tattgcactg ctggcgtgtg
      421 cgggtccgat gggcattggc gctatcgatt acgccattaa cggtggcata caaccgtcac
      481 gggtggtggt ggtcgatatc gacgacaaac gcctggcgca ggtgcagaag ctgctgccgg
      541 tggatctggc ggccagtaaa ggcattgagc tggtgtatgt gaataccaaa gggatgagcg
      601 atcctatcca gatgctgcgg gcgctgacag gagatgtcgg gttcgatgac atttttgttt
      661 atgcggcggt gcctgctgtc gttgagatgg ctgacgaatt actggcggaa gatggctgtc
      721 tgaacttctt tgccgggctg acggataaaa acttcaaagt gccgtttaat ttctacaacg
      781 tccattacaa cagcacgcac gtagtcggta catccggcgg ttcaacggac gacatgaaag
      841 aggcgattgc ccttagcgcc acaggacagt tacagccgtc gtttatggtg acccatattg
      901 gtggcctgga tgcggtgcca gaaaccgtgc tcaatctgcc ggatatccct ggcggtaaaa
      961 aactcattta taacggcgtg accatgccgc tcactgccat tgccgatttt gccgaaaaag
     1021 gcaaaaccga tccgctgttt aaagagttgg cgcggctggt tgaggaaacg cacggcatct
     1081 ggaatgaaca ggccgagaaa tatctgctgg cacaatttgg cgttgatatc ggggaggccg
     1141 cgcaatgatg tccctggcgt ggccattatt tcgcgttacg gaacaggcag cgttggctgc
     1201 ctggccgcaa accggatgtg gcgacaaaaa taaaattgat ggcctggcgg tcaccgcgat
     1261 gcgccaggca ttaaacgacg tcgcttttcg tggacgagtg gttatcggcg aaggagaaat
     1321 tgaccatgcg ccgatgctgt ggattggcga agaggtgggc aaaggcgatg ggccagaagt
     1381 cgatatcgcg gttgatccca ttgaaggcac gcggatggtg gcgatggggc agagcaatgc
     1441 gctggcggtc atggctttcg ctcctcgaga tagcctgtta catgcgcccg atatgtatat
     1501 gaaaaagctg gttgttaatc gactggctgc cggggcgatt gatctgtctt tgcctctggc
     1561 ggacaacttg cgtaacgtgg cgcgggcgtt aggtaaaccg ctggacaagc tgcgcatggt
     1621 tacgctggat aaaccgcgtc tgagtgccgc gattgaagaa gcaacacagc tgggcgtgaa
     1681 ggtttttgcc ctgccggatg gcgatgtcgc cgccagcgtg ctgacctgct ggcaggataa
     1741 tccttacgat gtgatgtaca ccatcggcgg tgcgccagaa ggcgtgattt ctgcctgtgc
     1801 cgttaaagcg ttaggcgggg atatgcaggc cgagctgatt gacttttgtc aggctaaagg
     1861 ggattacacg gaaaatcggc agattgcaga gcaggagcgc aagcgttgta aggcaatggg
     1921 cgttgacgtt aaccgtgtgt actcgctcga tgaactggtg agggggaacg atatcctctt
     1981 tagcgctacg ggcgtgacgg gcggcgagtt ggtgaacggt atccaacaga cggcgaatgg
     2041 ggtgcggacg cagacattac tgatcggcgg cgcggaccaa acgtgtaata taatagactc
     2101 cctgcattga tggtgatcta tcggaagacc aatggcgacg ctgacagaag atgatgtgct
     2161 tgagcaactg gatgcacagg acaatttatt ttcatttatg aaaactgcgc attctatttt
     2221 gctccagggg atacgccagt ttctgccgtc gctgtttgtc gataacgatg aagagatcgt
     2281 cgaatatgca gtgaagccgt tactcgccca aagcggcccg cttgacgata ttgatgttgc
     2341 gctgcgtttg atttatgcgc tggggaaaat ggataaatgg ctgtatgccg atatcacgca
     2401 cttttcccag tactggcatt acctgaacga acaggatgaa acgcccggat ttgccgatga
     2461 tataacctgg gattttatca gcaatgtcaa cagtataact cgcaatgcga cgctctacga
     2521 tgcgttaaaa gcgatgaagt tcgccgattt cgccgtctgg tcagaggcgc gttttagcgg
     2581 aatggtcaaa acggcgctga cgctggcagt aacgaccacc ttaaaggaat taacgccgtg
     2641 aaaattgaat taacggtgaa tgggctgaac gtgcaagcgc agtaccatga tgaagagata
     2701 gaacgcgtgc ataaactgtt gctgcgtatg ctggcggcct tacagacggt aaatccgcag
     2761 cggcgtacgg tggtttttct ttgcgctccg ccggggacgg gtaaatctac cctaaccacc
     2821 ttctgggaat atctcgcgca acaagacccg gagctgcccg ctattcaggc gctcccgatg
     2881 gatggttttc accattacaa tagctggctg gacgcgcatc aattgcgccc cttcaaaggc
     2941 gcaccagaga catttgacgt tgcgaaactg gcggaaaatc tgcgccaggt ggtggaaggg
     3001 gattgtacgt ggccgcagta cgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac
     3061 gttaccgcac cactcgtcat cgtcgaagga aactggttgt tgctggacga cgagaagtgg
     3121 tgccagcttg cgcaattttg tgatttctcg atctttatca acgcgccagc tgctgcgttg
     3181 cgggagcgtc tggtggggcg caagctggcg ggaggggtat cgctggcaga tgcagaagcc
     3241 ttttatgacc gtaccgacgg gccgaatgtt cgccgggggc tggaagagag tctgcctgca
     3301 aatttgacct taatgatgac cgctacgggt gaatatcgtc tggtggatta acgttcctga
     3361 ttacaccatc ttccagccgc accacacgag agaaatgacg ccgggtaaat gcggcgtcat
     3421 gactgattgc taccacgctc gtgcctcgct ggcggcactt ctccagccag ctttcaaaca
     3481 cgctcaacca attttcgtca aaatcccgac tcggctcgtc cagcagtaat agtggcggag
     3541 agagtgcttc caggcaggcg acggcgacca tccgtcgctg cgcattatgc agatccaacg
     3601 ggtgcgagtt ggcagtgtct gctaactgac aacattgcag ggcagcatgg gtacgttgag
     3661 tgatttcatc cgccgggcat ttttgcagtt tcaaaccaaa tgcgatctcg tcagcgacgg
     3721 tgctgtgaaa aagttggttt tccgcttcct gaaacagtac gccaaccttt gccgcgcgct
     3781 ggcggttttt caggtttttc atcgcctgtt gttgcagcat aaccgtaccg gctgtgggcg
     3841 taagaagacc tgccattact cgtaacagcg tcgatttccc tgcgccattg tcaccggtca
     3901 gcgccaacca ttcaccttgt ttgagttgca gagaaatgtc gcaaagacaa tccgttgccg
     3961 cgccaggcca gcgataagat atcttattta acgttaacat agtggctgga gcgctccgtt
     4021 ttgcaattgc cacgcttgct ggcagtagtt caggaaaggt gtgcgatgac gttcaaaaag
     4081 aataatcagc gaaccacgtt caaacgccca gtgttgtaag cgctgcaaca gcatttcgct
     4141 ggcctgcggt gtcaggcggc taaaggcttc atcaagaatt aagagctttg gctgcatcgc
     4201 gatggcgcag gcgatgatca cgcgctgtgt ctctccaccg gagagtgttg ccgggtgacg
     4261 atggcgcaac ggctggcact cggtcagagt cagggcggca tcaatccgcg ccataatctc
     4321 tgcttcagca agacacagat tttctggccc aaaggcaact tcttcttcca cgctaaaggt
     4381 acagccggag agctgcaaat agggcgattg ctggacgagc tggatggtgg cggattgttc
     4441 attaagcggg agatgcccaa tgggcgtacc gaggagcgtc cccgtgcctg ttatctctct
     4501 tggcagaaag tccggatacc agcccgccat cagctgtgcc agcgtacttt tgccactgcc
     4561 gttgtcacca aagatggcga ccatccccgg tttgacatag tgaaagtcat aacagaacgg
     4621 aggatgcgtt gagtgtgtcg ggcaataacg gaactgttct aacgtaacca tatccacgct
     4681 ccgaattctg tcagcatcag caagatcatg gtgtaacgcg ctacgcgttg tagggtgcta
     4741 tcggctggtg cccacaacgt tgttcgtcgg ttgtggatcc gaaatgcccg catatccagc
     4801 gccgcaccac ggacagccag atcgttaagc gcattgtgtg ttagcggaat aattaacgcg
     4861 ggcatagcac gtaaacgctg ataccagccc tcatccagcg gcacgccgcg ggcacgctgc
     4921 gcttcatgaa taatcgctaa ttgccgtttt aactgctcaa cgaccaataa cggcccggca
     4981 aacagataag cgacgcctgg cggcaggcga gaagcaaaca gagcgcgaat aaaacattgc
     5041 acggggacgt actgcattca caactgtgag gttgaaacaa tcgccagaat cagcaaccag
     5101 agcgtaatag catgcgacca ccgttctggc gagcgtggcg tgccgcttag ccattctggt
     5161 agccagccac cgtgtaccag ccacagcccg gctcccagcg agaacatcag ccagacgaca
     5221 tattttgccc gccgccgcgt ggcctttagc gcaataaggc agaaaaaggt cgccgcgcta
     5281 tagatcggca aaatggtctg cgcgggcagg ataagcgtgg tgcaggccgc taatgcccac
     5341 agtgttaatg aggtaaatgg atgcatttag cgcacggcag ccattgccgg aaaatggcgc
     5401 gtggtgcgaa tcggcagctg gcgcagtaaa agccagacaa tcaccgctgt aagaatttta
     5461 tcgaccaggt tcgcgcctat caccgtaatc gccaccgact ccaccaggtt ttgccccatc
     5521 gagtgcatcc aggcaacgaa caaatcggct ccgctaccgg tgacaccgcc gaacagcgca
     5581 gtacgcagcg gtacagcgac gaccgttacc gcaagcgtaa taatcacgcc gctgacaacg
     5641 acttttggca gggtgcgaaa ccaacccgct cgcgccagcc aaccggagac caggccgatg
     5701 accatcgcga cgggtgcaaa tgcggcggcg atcgggtcgg tcagcaatcc ccagagtaaa
     5761 ttggtcagta agccggtcag cattccgatc accggaccaa gcagtaccgc gctaataagt
     5821 gtgccgatgg agtcgagaaa tataggtaat ttgaccatgc tggccagttg cccgccgatc
     5881 atattgatgg cgatagatat aacgatcaac accagtgcct gactggaaaa gtggctacgc
     5941 gccataagaa aacctctttg aaagtagtta tttaacgggg gaaatatgca actcgtcgta
     6001 accagaaagc gcgcaaggtt ggcggctaaa cgccagttcc tcaaggctac cgatcaccag
     6061 ttcaagcgtg gtccgcacgg tacagccggg catcatatga ccgccaagca tcgtgccgtg
     6121 tggatcggaa acgcagagat gaagatgctc accactctgt tccagcgtgc cgtttagtga
     6181 gatcacttcg aatgtaccgt tcaggagcgt ggtgccttct tgcccggcat agcgcaaggc
     6241 gacgtcagtc aggctaccgg tacagcctgc aatctaggcg gcatgaagtt gatgttgctg
     6301 tgcaaaagcg tgtagctggg aaaaaacttc ctgcccgggg aggaggcgca gtgcgtaaaa
     6361 gcgtgcggag gaagaatatg agtagggcaa tgtcatcatt ggagtactcc gaaactcgga
     6421 cgtattgcgg ataatatcga cgctgcatca gtcatattct gatataagtc agggtttgtg
     6481 acggcacaac gaaaagattt tgctacgctt attcgtgatt ctgcaaagtg gaataaattc
     6541 cacaggggcg gggctgaaaa cgtgacgtaa gtcagataat tgctttcttt gactggacaa
     6601 tcattccttt tattccacgt ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt
     6661 tcgacaatta accaatcagt cgcagtttgc gacaggtaag gtttccccgg acgatttgct
     6721 ggattactct gtctggcaaa tttgctcagg gaaaaccttg caggagatct atgaccgtac
     6781 gcgtagcgat aaatggcttc ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat
     6841 ccggacgccg ggcggaaatt accgtggtgg caatcaacga actggcggat gctgcgggca
     6901 tggcgcattt gttgaaatat gacaccagcc atggccgttt tgcatgggaa gtacgacagg
     6961 aacgcgatca actttttgtt ggtgatgacg ccatccgcgt attgcatgaa cgttcactgc
     7021 aatcgctccc ctggcgtgaa cttggcgttg atgtagtcct cgactgcacc ggcgtatatg
     7081 gctcccgcga gcatggcgaa gcgcatattg ccgccggggc caaaaaagtg ctcttttcac
     7141 atcctggcag taacgatctc gacgcgaccg ttgtttacgg cgtcaatcag gatcaacttc
     7201 gtgcggaaca ccgcatcgtt tctaacgctt cctgtaccac gaattgcata attcccgtca
     7261 tcaaattgtt agatgatgcg tacggtattg agtccggcac tgtgaccaca attcactccg
     7321 ccatgcacga tcaacaggtt attgatgcat accatcctga cctgcgtcgc acccgggcag
     7381 ccagccagtc gatcattccg gtcgatacta aactggccgc tggtatcaca cgattttttc
     7441 cgcaatttaa cgatcgcttt gaagcgattg cggtacgtgt gccaaccata aatgtgacgg
     7501 caatcgattt aagcgtgacg gtgaagaaac ctgtaaaagc caatgaagtc aacctgttgc
     7561 tgcaaaaagc agcacaaggt gcatttcatg gtatagttga ctatacggaa ttgccgttgg
     7621 tctctgtaga ttttaaccac gatccgcaca gtgccattgt cgatggcacc caaacccggg
     7681 tcagtggcgc acacctgatc aaaacgttgg tctggtgcga taacgaatgg ggctttgcta
     7741 accgaatgct cgacacgacg ttagctatgg ctactgttgc tttcaggtaa gacgcaagca
     7801 gcgtctgcaa aacttttaga atcaacgaga ggattcacca tgtctgtaat taagatgacc
     7861 gatctggatc ttgctgggaa acgtgtattt atccgtgcgg atctgaacgt accagtaaaa
     7921 gacgggaaag taaccagcga cgcgcgtatc cgtgcttctc tgccgaccat cgaactggcc
     7981 ctgaaacaag gcgcaaaagt gatggtaact tcccacctgg gtcgtcctac cgaaggcgag
     8041 tacaacgaag aattctctct gctgccggtt gttaactacc tgaaagacaa actgtctaac
     8101 ccggttcgtc tggttaaaga ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt
     8161 gttctggaaa acgttcgctt caacaaaggc gagaagaaag acgacgaaac cctgtccaaa
     8221 aaatacgctg cactgtgtga cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg
     8281 caggcttcta ctcacggtat cggtaaattc gctgacgttg cgtgcgcagg cccgctgctg
     8341 gcagctgaac tggacgcgct gggtaaagca ctgaaagaac ctgctcgccc gatggtggct
     8401 atcgttggtg gttctaaagt atctaccaaa ctgaccgttc tggactccct gtctaaaatc
     8461 gctgaccagc tgattgttgg tggtggtatc gctaacacct ttatcgcggc acaaggccac
     8521 gatgtgggta aatccctgta cgaagctgac ctggttgacg aagctaaacg tctgctgacc
     8581 acctgcaaca tcccggttcc gtctgatgtt cgcgtagcaa ccgagttctc tgaaactgca
     8641 ccggctaccc tgaaatctgt taacgatgtg aaagctgacg agcagatcct ggatatcggt
     8701 gatgcttccg ctcaggaact ggctgaaatc ctgaagaatg cgaaaaccat tctgtggaac
     8761 ggtccggttg gcgtgttcga attcccgaac ttccgcaaag gtactgaaat cgtggctaac
     8821 gctatcgcag acagcgaagc gttctccatc gctggcggcg gcgacactct ggcagcaatc
     8881 gacctgttcg gcattgctga caaaatctcc tacatctcca ctggcggcgg cgcattcctc
     8941 gaattcgtgg aaggtaaagt actgcctgca gtagcgatgc tcgaagagcg cgctaagaag
     9001 taaaaaatca cagggcaggg aaacctgcac ttgtttcagc gcgcttttag agcacgcatt
     9061 ttttcatact ctaaataatt cgagttgcag gaaggcgaca agagagtgaa tccccaggag
     9121 cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta
     9181 ttatgagtat aaggcccgac gatacaggac aagagacatg tctaagattt ttgatttcgt
     9241 aaaacctggc gtaatcactg gtgatgacgt acagaaagtt ttccaggtag caaaagaaaa
     9301 caacttcgca ctgccagcag taaactgcgt cggtactgac tccatcaacg ccgtactgga
     9361 aactgctgct aaagttaaag cgccggttat cgttcagttc tccaacggtg gtgcttcctt
     9421 tatcgctggt aaaggcgtga aatctgacgt tccgcagggt gctgctatcc tgggcgcgat
     9481 ctctggtgcg catcacgttc accagatggc tgaacattat ggtgttccgg ttatcctgca
     9541 cactgaccac tgcgcgaaga aactgctgcc gtggatcgac ggtctgctgg acgcgggtga
     9601 aaaacacttc gcagctaccg gtaagccgct gttctcttct cacatgatcg acctgtctga
     9661 agaatctctg caagagaaca tcgaaatctg ctctaaatac ctggagcgta tgtccaaaat
     9721 cggcatgact ctggaaatcg aactgggttg caccggtggt gaagaagacg gcgtggacaa
     9781 cagccacatg gacgcttctg cactgtacac ccagccggaa gacgttgatt acgcatacac
     9841 cgagctgagc aaaatcagcc cgcgtttcac catcgctgcg tccttcggta acgtacacgg
     9901 tgtttacaag ccgggtaacg tggttctgac tccgaccatc ctgcgtgatt ctcaggaata
     9961 tgtttctaag aaacacaacc tgccgcacaa cagcctgaac ttcgtattcc acggtggttc
    10021 cggttctact gctcaggaaa tcaaagactc cgtaagctac ggcgtagtga aaatgaacat
    10081 cgataccgat acccaatggg caacctggga aggcgttctg aactactaca aagcgaacga
    10141 agcttatctg cagggtcagc tgggtaaccc gaaaggcgaa gatcagccga acaagaaata
    10201 ctacgatccg cgcgtatggc tgcgtgccgg tcagacttcg atgatcgctc gtctggaaaa
    10261 agcattccag gaactgaacg cgatcgacgt tctgtaagtc attcctttct gcttatctca
    10321 aggcccgctc tgcgggtctt tttttcgcca aaagcaaggt gattcagatg agaaatctgt
    10381 gatctatttg gcaaaattat gctttattgt ttacccttgt cagactgccc gtcataaggc
    10441 ggcggagtgt atttctccat tttgagtcag ttgaaaagga atattgaatg gaagatttga
    10501 atgttgtcga tagcataaac ggcgcgggaa gctggctggt agctaaccag gcgctgctgc
    10561 taagttatgc agtaaacatc gtggcggcac tcgcgatcat catcgttggt ttgattatcg
    10621 cgcggatgat ttccaacgcg gtgaatcgcc tgatgatctc ccgtaaaatc gatgccactg
    10681 ttgctgattt tctttctgca ttagtccgtt acggtattat cgcctttacg ctaatcgctg
    10741 ccctgggacg cgtgggtgta caaacagcgt cagtcattgc tgtactcggt gccgcaggct
    10801 tagctgttgg tctggctttg caggggtcac tttctaacct ggccgctggc gtgttacttg
    10861 tcatgttccg cccgttccgt gccggagaat atgttgacct cggcggcgta gccggtactg
    10921 tgctgagtgt gcagattttc tccaccacca tgcgtactgc agacggtaaa attatcgtta
    10981 ttccgaacgg taaaattatt gccggaaata ttattaactt ctcccgcgag ccagttcgcc
    11041 gtaacgaatt tattattggc gtggcgtatg attccgatat cgatcaggtt aagcagatcc
    11101 tgaccaatat tatccagtct gaagatcgca ttttgaaaga tcgcgaaatg actgtgcgcc
    11161 tgaacgaact tggtgcatcg tcgattaatt tcgtggtccg cgtctggagc aacagcggcg
    11221 atctgcaaaa cgtgtactgg gatgtgctgg agcgtattaa acgtgaattt gatgccgccg
    11281 gtatcagctt cccgtacccg caaatggatg tgaactttaa gcgggtgaaa gaagacaaag
    11341 ctgcgtaatc aacgctgagg ccagataata ctctctggcc tctctcttat tagttattct
    11401 gattgccaat taatattatc aatttccgct aataacaatc ccgcgatata gtctctgcat
    11461 cagatactta attcggaata tccaacgtgt tttcttatta ctttcaaggt cttgctcttg
    11521 gggcagctat gattctgccg ctcggcccac aaaatgcttt tgtgatgaat cagggcattc
    11581 gtcgtcagta ccacattatg attgccttac tttgcgctat cagcgatttg gtcctgattt
    11641 gcgcggggat ttttggtggt agcgcgttat tgatgcagtc gccgtggttg ctagcgctgg
    11701 tcacctgggg cggcgtagtc ttcttgctct ggtatggttt tggcgctttt aaaacagcga
    11761 tgagtagtaa tattgagtta gctagtgccg aagtcctgaa gcaaggcaga tggaaaatta
    11821 tcgccaccat gttggcagtg acctggctga atccgcatgt ttacctggat acttttgttg
    11881 tactgggcag ccttggcggg cagcttgacg tcgaaccaaa acgttggttt gcgctcggga
    11941 caattagcgc ctctttcctg tggttctttg gtctggctat tctcgcagcc tggctggcac
    12001 cgcgcctgcg cacggcaaaa tcacagcgca ttatcaatct ggtagtggga tgtgtcatgt
    12061 ggtttatagc cttgcagctg gcgagagacg gtattgctca tgcacaagcc ttgttcagtt
    12121 aggcgctatc tgatggaaaa ataaaacaga ggcgctaagc ttgcctccag aggtcctgaa
    12181 ttttgggcaa gtcaattaac acggagagac taacgtgaag ttcaaagtta tcgccctggc
    12241 ggcattaatg ggtattagcg ggatggcagc gcaggctaac gaattgccgg atggaccgca
    12301 tattgtcacc tccggtacgg caagtgtgga tgcggtgcca gacattgcca ctcttgcgat
    12361 tgaagttaac gtggccgcga aggatgccgc tacagccaag aaacaggcag atgagcgcgt
    12421 cgcacaatac atttccttcc ttgaactcaa tcagatcgcg aaaaaagata tcagctcagc
    12481 gaatttacgc acccagccag attatgacta tcaggatggt aaaagtatcc tgaaaggcta
    12541 ccgagctgtg agaacggtgg aagtcacgct ccgtcagtta gacaaactga attccttgct
    12601 ggatggcgcg ctgaaggcgg gtcttaacga aattcgttct gtgtcgctgg gcgtggcgca
    12661 gccggatgcc tataaagaca aagcgcgtaa ggcagcgatt gataacgcga ttcatcaggc
    12721 gcaggaactg gcgaacggct ttcatcgtaa actggggccg gtatatagcg tgcgctacca
    12781 tgtttccaac tatcagccca gcccaatggt gcggatgatg aaagccgatg ccgcgccggt
    12841 gtctgcccag gaaacttacg agcaggccgc tattcagttt gatgatcagg tcgatgtggt
    12901 cttccagtta gaacctgtgg atcaacaacc cgctaaaaca cctgcagcac aataatactt
    12961 gtagcccgac gtattcgtcg ggctaatgcc tttacccgat attgcctcct ttttctgtaa
    13021 ttttctgtaa aaccatgacg taatcaccta tctttgctcg cggtttcatc aaggataggc
    13081 tgactgaaac ggttaagcgg acaaggatgc ggggaggggg gatggacatt tttatttcga
    13141 aaaagatgcg caatttcatt ttattagcgc aaaccaataa tattgcccgg gcggcagaaa
    13201 aaatccatat gacggcttcg ccatttggta aaagcattgc cgcgctggaa gaacaaattg
    13261 gctatacgct atttacccgc aaagataata atatcagcct caataaagcc ggtcaggaac
    13321 tgtatcaaaa actgttcccg gtttatcaac gactttctgc gattgataat gaaatccata
    13381 actcagggcg tcgttcacga gaaattgtga ttggcataga taatacttat ccgacaatta
    13441 ttttcgatca gttgattagt ctcggagata agtatgaagg cgtgaccgcc cagccagttg
    13501 agttcagcga aaacggcgtt atcgataatc tttttgaccg tcagttagat tttattatct
    13561 ccccacagca tgtatctgca cgagttcagg aactggaaaa cctgactatc agtgaattac
    13621 caccgctgcg attgggcttt ctcgtttctc gccgttatga agagcgtcaa gagcaagagc
    13681 tgcttcagga gctgccctgg ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa
    13741 tgattgatgc gaatatgcga ccttgtggga tcaatcccac cattatttat cgtccctaca
    13801 gttttatggc caaaatcagc gccgtggagc gcggacattt tctgacggtg atcccgcatt
    13861 ttgcctggcg actggtgaat ccggcaacgc tgaaatattt tgatgcgccg cacaggccga
    13921 tgtatatgca ggaatatctt tattcaatca gaaatcatcg ttataccgcc acgatcttca
    13981 gcatattgct gaagatcgtg acgggacaaa ccattaaccc agcatcgagc cggttgcaat
    14041 taaattacgg tgtaagtcga agacgtggct aagatcgtgg tgaatatgtc cgccgggcgc
    14101 attttccaga tagcgatgca gataatcccg atacatagga tgtgcacaat tatcaatgat
    14161 agtgcgggcg cgttgaagcg gggaaagacc gcgcagatcg gcgatccctt gttcggtgat
    14221 gatcactttg acgctgtgtt cgctgtgatc aacatggctg cacattggca cgacggttga
    14281 gatcttgcct tctttagcaa tcgacggagc cataaagatc gacagatacg cgttgcgttc
    14341 aaaatcaccg ctgccgccga tgccgttcat cagatcgacc ccggctacgt gcgttgaatt
    14401 tgcatgcccg taaatatcaa actccaggcc gacgttcaga gcgatgacgc ccagacgacg
    14461 gatgatttcc gggttattgg aaatctcctg cggacgcaac acaatgcggc tggcaaagta
    14521 atccatattg tcgtaaatct tgcgcaggga atcggccgag attgtcaggc tggaggcgct
    14581 ggccccgctg attttgccgg tttccagtaa atgcaccacc gattcctgta gcacttccga
    14641 atacatcatg aacggaggaa tttccgggtt ttcccccaga cgtgccatta ccgcattatt
    14701 gatattgccc acgccacttt gcagcggcag aaattccggc ggaatacgcc catgcgccat
    14761 ttcctgcaat aagaacgtga ccacgttatc ggcaatctgc tggcacatgg gattttgctt
    14821 atccagcata ttaccggcgt cgggcaagtt ggtttccacg acggcgacaa tctttttcgg
    14881 atcgatttgc acatagcggg taccgacgcg atccattgca tggaagatcg acacgctatt
    14941 gcgccgcggt ggcgcgccag gaatcacaat atctgccagt tctgcaacgc gcggatcgtg
    15001 atagtgatta agttcaatga tcactttctt cgctcgcagc agctaggtcg gcgcattacc
    15061 gatcccgctg gttaaccaga ctcgaccatc cggtgccagt gccgatgctt caatgacggc
    15121 aacatcaatg tcgccgaaga aaccgtaatt gaccatttgc gccacttcgc tcaaatgcag
    15181 gtcaacgaaa ctcaccgcgc cctgattgat ctttttacgt aaactggacg atgtttgata
    15241 tggcgcacgc caggaaacag catcggcgtc agaaagtaca tcgtcagcgg cggcgctgat
    15301 tgacgccccc gtcagcaggc gaatttgata cggctttttg gcctcatgct gttcgttagc
    15361 tctgcgggca atcgcggcgg gtagggcttt cggcgaaccc gctggggtaa agccgctaaa
    15421 tgccaccatg tcgttatgct ggataatttc tgccgcttca tcggcggtca tccttgtcca
    15481 ctgagtttcc atttttactc cttcgttcgc agggattaat gaccaacgaa attaggttta
    15541 cgtttttcga ggaaagcgtt catcccttcc tggtaatctt cgctgtcata aaccgcgcgg
    15601 cgcatcccct gaatacgttc aaattcatcg gagttcatgg tgtgtgcttc gcccagtaca
    15661 cgcagctctt ctttgataac ggcaatggct aacggcgctt tctcagagat gtggtgcgcc
    15721 atttgtaagg tgaaatcttc cagttcttcc acttccacaa catggttgag gatgccgaca
    15781 gccagcgcgc gctgggcggt gattggcgaa gcggtaaaaa tcagctcttt gacaatgtgg
    15841 aagcccgcgt cgcgggtcag gttgtgaatg ccgaccaggt tatacgggac gccgaggttt
    15901 acaggcgtca ttgagaaggt tgaggtactg gcggcgatga tcagatcgga actcatgatc
    15961 atttcaaatg cgccacccca aacactacct tccaccatcg aaatgatcgg tttcgggaat
    16021 ttttggatca tgcgggtgat ttgacgcaat ggatcatcat aggagagcgg atcgcgaccg
    16081 ccagacggca gttcgtgaat atcgtgacct gcggagaaga ctttggatcc acttggcgcc
    16141 cgcaaaatga tacagcgaat ttccggccgg ttgagatcgc ttaacgcctg cataagatca
    16201 tcaataaaga ctttacttaa ggcattaagt tttcgtccat agttaaactc aatgaccgcc
    16261 actttgttga tagtggcaac gttcacatac tgataagaca taaaaattcc tttaatcaaa
    16321 atattgcgtc tggataaatt cactgagctg ccgcaggccg gtgcgcggtg agagcgtatt
    16381 gtttttgacc gctaaaagcg tctggcggta atagcgatcg aaatcttcat tcgcgaacag
    16441 gtgattcagt acttcttctt cggtctgctt acgcagccat tccaccgatt gttgttgccg
    16501 cacttgttgt aaacgaccac tggcagttag cgcggttttg aagtcgatga tggcgtgcca
    16561 gatctcatcg attccacgtt tttccagtgc gctacaagtc agaccccgtg gctgccattc
    16621 gtcgtattta cgtcgcagaa tatgcagggc actctcgtac atatgccggg caatggcgac
    16681 attggtatgg ttatcgccat cgtctttgtt gataacgagc agatcagcca cttccatcag
    16741 ccctttttta atgccctgca gatcatcgcc gccaccggca atttgcaacg agataaaaca
    16801 gtccaccatg cgggcgactt ccgtttccga ctgcgcgatg ccaaccgttt cgacaatcac
    16861 tacgtcataa cccgccgctt cgcataacag cattaattcc cgtgctcgct gactggcacc
    16921 gcccagatga ccggaggagg gtaccgggcg aataaacgcc gcctcggcac gcgccaggtc
    16981 attcatgcgg gttttatccc cgagaatgct accgccagtg accgggctgc tgggatcgac
    17041 cgcaataaca gcaaccttta atccctcacg aatcaacaac atgccaaagg cttcaagaaa
    17101 ggtacttttc cccgcgccgg gtgtgccggt aacgcccagt cgcagggcgt taccacagaa
    17161 cggcataata gcgtcaagca gctgtgtact tagcgcctga tgacgtgggt gacggctttc
    17221 caccagcgtc atggcttggg cgagcgtggc acgctcaccc tgacgtaagc ggcgaatact
    17281 ttctgccagc gtggcttcat taatcatgat gctggcttat cagattcagt acgtcgcgca
    17341 cactgtcgag cataggtgta cctggaccat aaatcgccgc cacgccgcgc tcttgcagga
    17401 aggcgtaatc ctgcggcgga atgacgccac ccgcgaccac gcagatatct tcgcgtcccc
    17461 attttttcag cgcttcgacc agttccggga tcagcgtttt atgaccggca gccagtgagg
    17521 atgcgcccac tacgtgaacg tcgttttcta cggctaggcg ggcgatctct tcaggtgtag
    17581 agaacatcgg gcttaaatct acgtcgaaac cgagatcgga ataggcgctg gcgatcactt
    17641 tcgcgccgcg atcgtgtcca tcctggccca tcttagcgat cagaatgcgc gggcgacgac
    17701 cattgtcggc aaggaactgc tccgtttgcg caacaatggc atcgaactcg gaggccgatt
    17761 tctcagactg atgatagctt tgcgcaatca cgccggtaac acactggctt ggcaccagat
    17821 aacggtcgaa agcgacttcc agcgcatcgg aaatttcacc cagggtggcg cgaacgcgag
    17881 cggcattaac agcggcagcc agcaggtttt cgttatgctg tgcggcgtga gtcagggcgt
    17941 tcaacgcggc ggttacggcg gcatcatcac gggtggcgcg aatgcgttcc agcgaagcaa
    18001 tttgctcgtt acgcaccatc acgttgtcga tctcaagtac atcggtttcg tcttcgtgat
    18061 ccagtttgta cttgttgaca ccaacgatga cacgcttgcc ctggtcgatc agcgactgtt
    18121 cgcgcgctga ggcctcttcg atcattcgtt ttggcagacc tgcttcgatc gctttcgcca
    18181 tgccaccggc ttcgtcgatc tgttggataa tagctctggc ttgtttgacg atttgatcgg
    18241 tcagcgactc aatgtaatag gatccggcca gtggatcgac ggtgtggcag agttctgatt
    18301 cttcctggat gatgatctgg gtgttgcggg caatgcgtgc tgagaaatcg gtaggcaaac
    18361 caagcgcttc gtcaaaggcg ttggtatgca gtgactgagt accgcccagc gtcgcagcca
    18421 gtgcttcaat ggtggtgcgg ataacgttgt tatacggatc ctgttcagtc aggctccagc
    18481 ctgaggtctg gcagtgggta cgcagcgcca gtgatttcgg atcctgtgcg ccaaatccac
    18541 tgaccgcttc gctccataaa taacgtgccg cacgcaacat ggcgacgttc ataaacagat
    18601 ccatgccgat accgaagaat aacgacaggc gaggagcgaa gtcatcaatt ttcagtccgg
    18661 cagagattgc tgctttgatg tactcaatcc catcggcgag cgtaaatgct acctgctgca
    18721 cgcagttggc acccgcttcc cccatgtggt aaccgctgat actgatggta ttaaatcgcg
    18781 gcatgttgcc ggaacaccag gcgatgatgt cggcgataat gcgcattgac ggttttggtg
    18841 ggtaaatata ggtgttgcgg cagaggtact ctttgagaat atcgttttga atggtgccgg
    18901 tcagtttatc aggtgtaaca ccttgctctt ctgcggcgac gatataaaac gccagtactg
    18961 gtagcactgc gccattcatg gtcatcgaaa ccgacatttt atccagcggg atctggtcga
    19021 acaggacttt catatcttcc acggtgtcga tagcgacgcc cgctttgccg acgtcacccg
    19081 ccacgcgtgg gttatcggag tcgtagccac ggtgggtggc aaggtcaaac gcaacggaaa
    19141 gacctttttg cccggcggcc aggttacggc gataaaaagc gttggactct tttgctgttg
    19201 aaaaaccagc atactgacgg atggtccacg gttgggcggt atacatagtg gcacgcgggc
    19261 cacgaacgta gggcggcaaa ccaggaaggg tacctgtcac ctccagatta tcgagatcgg
    19321 cttcggtata cagcggcttg atggcgatcc cttccgcggt ttgctgaacc agcgagtcga
    19381 cagttttctc ccgacggctc aattccttgt tggcaagctg ttgccactcc tgctcgttag
    19441 acattatttg ctccgtaaaa cgcaatcaaa atgagggtat ttgcgcttac ggtgagcttt
    19501 ttacgtgggt ttgtcgccag caaaaaaaat gcattcagac gtcgtttttt gccgcctgag
    19561 atttgtctat tttgtgatgt gattcaagga atacaaaaag aagtgaggac aacgcctgat
    19621 atgtgctatc aggcattatt tgatggatta atcctgacga aggactttgt gaccataatc
    19681 gagtaacgca tcagtgactt tacgcatcat gcggctttca ggagcaaagc ggtgccagta
    19741 gagcatccgt cgttgaaata gcccaggcgt taagtcaatc agttcaccgc tggccagctc
    19801 tttctcgatt tgcaggtgcg ggatcataca gcaggtggtg ccctggcgag caagttgtac
    19861 gaacgcttct gaagaattaa cgatatggca gggtacgctg ccaggaggaa gatcgaagtt
    19921 ttgctgcaaa aaggcctggt gcatatcgtc aagatggtca aacgcgacca ctggcgcttt
    19981 caataatgcc gaacgcgtta cgccgttagg aaaatatttt tcggcaaacg gttttgagct
    20041 gacgaacaga tagtcgagcg caccgagttt atcgacaaga caactcggca gcgcctgatg
    20101 ttgaatactc accgcgccga ccacttcgcc gcggcgcaga cgttcctgag tacgggtttc
    20161 atcttctacc tgcaagttga ggcggatagg cgaatcagcc aacacaggag ccagtgccgg
    20221 aagcaaccac gtcgccagac tgtcggcgtt gaccgccagt gaaagcagca gcggagtcga
    20281 accggtttgt tcatcgccca gccactcttc ttccagcaac tccacctggc gcagcagtgc
    20341 cagcagtttt tgcccttgtt ccgtcgggcg cggcggtacg gtacgcacca acagcggctg
    20401 cccgaacata ttttccagtt gcttaatgcg ctgtgagacg gctgattgtg taatgcacag
    20461 cttttgtgcc gcgcgctcaa atcctcgttc acgtatcacc gcatccagtg cctgtaatgt
    20521 tctgtagtcc gggcgtttca ttgctgttgt ctgctcctgg taaatgcttt ttcctgcact
    20581 atgacacaat tttgtgtcag gttgcatata ccgaagccgt cggcgggtaa catttgtatc
    20641 aatggttaaa cgcgcagctt aaaagatcag ggatttgcgc tattgcattt cgcttagcga
    20701 cttcgcgccc tggttatgtt gaatggcgtg gcgttattgc ctctgttcgc accgtggagc
    20761 ggcataccca gaccgttgta tgctttattt gcggggcgaa aggggatgcc cgccattgcg
    20821 cgatatttta tggatgagtt aaccacccgg cttgccaacg gggtctgaat cgcttttttt
    20881 gtatataatg cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc aggatgaatt
    20941 gaaaaaagca gtaggatggg cggcacttca gtatgttcag cccggtacca ttgttggtgt
    21001 aggtacaggt tccaccgccg cacactttat tgacgcgctc ggcacgatga aaggccagat
    21061 tgaaggggcc gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc tcggcattca
    21121 cgtttttgat ctcaacgaag tcgacagcct tggcatctac gttgatggcg cagatgaaat
    21181 caacggccac atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg aaaaaatcat
    21241 tgcttcggtt gcggaaaaat ttatctgtat tgccgacgcc tccaagcagg ttgatattct
    21301 gggtaaattc ccgctgccag tagaagttat cccgatggca cgtagtgcag tggcgcgcca
    21361 gctggtgaaa ctgggcggtc gtccggaata ccgtcagggc gtggtgaccg ataatggcaa
    21421 cgtgatcctc gacgtccacg gcatggaaat ccttgacccg atagcgatgg aaaacgccat
    21481 aaatgcgatt cctggcgtgg tgactgttgg cttgtttgct aaccgtggcg cggacgttgc
    21541 gctgattggc acacctgacg gtgtcaaaac cattgtgaaa tgatctgacg ggggaacctc
    21601 ccccgttaaa aaaattctct tcattaaatt tggtgacatg tgtcacgctt ttaccaggca
    21661 attgtcgatt gctctaaata aatcctctaa accagcatat tcatccaaga attacctttg
    21721 cgtgatattt cctcaacatc gcgacgcaaa cgttcatatt gccgcaatat tattttttga
    21781 tatgttgaaa ggcggatgca aatccgcaca caacatttca aaagacagga ttgggtaaat
    21841 ggcaaaggta tcgctggaga aagacaagat taagtttctg ctggtagaag gcgtgcacca
    21901 aaaggcgctg gaaagccttc gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc
    21961 gctggatgat gaacaattaa aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc
    22021 ccgtacccat ctgactgaag acgtgatcaa cgccgcagaa aaactggtcg ctattggctg
    22081 tttctgtatc ggaacaaacc aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt
    22141 atttaacgca ccgttctcaa atacgcgctc tgttgcggag ctggtgattg gcgaactgct
    22201 gctgctattg cgcggcgtgc cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa
    22261 actggcggcg ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca
    22321 tattggtacg caattgggca ttctggctga atcgctggga atgtatgttt acttttatga
    22381 tattgaaaac aaactgccgc tgggcaacgc cactcaggta cagcatcttt ctgacctgct
    22441 gaatatgagc gatgtggtga gtctgcatgt accagagaat ccgtccacca aaaatatgat
    22501 gggcgcgaaa gagatttcgc taatgaagcc cggctcgctg ctgattaatg cctcgcgcgg
    22561 tactgtggtg gatattccgg cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc
    22621 ggcaatcgac gtattcccga cggaaccggc gaccaatagc gatccattta cctctccact
    22681 gtgtgaattc gacaacgtcc ttctgacgcc acacattggc ggttcgactc aggaagcgca
    22741 ggagaatatc ggcttggaag ttgcgggtaa attgatcaag tattctgaca atggctcaac
    22801 gctctctgcg gtgaacttcc cggaagtctc gctgccactg catggtggtc gtcgtctgat
    22861 gcacatccac gaaaaccgtc cgggcgtgct aactgcgctg aacaaaatct tcgccgagca
    22921 gggcgtcaac atcgccgcgc aatatctaca aacttccgcc cagatgggtt atgtagttat
    22981 tgatattgaa gctgacgaag acgttgccga aaaagcgctg caggcaatga aagctattcc
    23041 gggtaccatt cgcgcccgtc tgctgtacta attccccttc tctgaaaatt aacgggcagg
    23101 tcactgactt gcccgttttt ttatcccttc tccacaccgt tgccgcgttg cgcattctac
    23161 tttgcgagtc tgctccagaa aataaatcaa agtcagatca atgcgttgtg tttatttaat
    23221 ggggatttac gatggcggga cagcatgggg ctgtaacggt aaagccctca ccgaagcgag
    23281 ggcttaaagg agaagggtta tgatgcgact tgtcatcata ctgattgtac tgttactgat
    23341 aagtttcgga gcttattaac agtcagtctc aagggaggag tcatcctccc ttacccttac
    23401 tcactaaatt aggtcaaaga atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc
    23461 tgaaccgaac tttttgatag ccacttcatc gcccacgttt actgccgtaa tagatccttt
    23521 ttcccaaaaa ttatctctgt tttgcatggg gtttttcgag cacgctcgaa gaaaagattt
    23581 aaaggtagat caatgcgttg tggttattta aagagagctt acgatagctg ggcagcatgg
    23641 ggctgtaacg gtaaagccct caccgaagcg agggcttgaa ggagaagggt tatgatgcga
    23701 cttgccatca tactgattgt actgttactc ataagtttcc cagcttatta acagtcagtc
    23761 acaggggagg agcaatcctc ccttaccctt actcactaaa ttaggttaaa gaatcaacga
    23821 tgtcaatcag ggcgatgcag gtgtatcgcc cttaccactc ccagactttc gacggtgtaa
    23881 ccaccgcagg aagagggata tcccactctt caacggggag tttttccacc aactgacaat
    23941 catgcgcata acccaccggt tgcgttttat agtgctgcca gttttgtaag gtccgatcat
    24001 aaaaaccacc gcccattccc agacgctgac cgtactcatc aaaggcgacc agcggtgtga
    24061 tcagcacgtc taatcgggaa aggggtagca cgtcacgcac atccagtttt ggctcatgga
    24121 tcttcaacct gttcatcacc agttcgcttt gcggatggta attcaggaac agcaaattac
    24181 cggcactaaa gggatgcaaa actggaagat atacgcgctt accggcgcgc cagagttgtt
    24241 ctattagcgg ctgggtgtcg agttcaccat caaaagagag gaatacagcg accgtatgcg
    24301 ccatcaccac cgggggataa gtcatcatcc gggtagcggc ttgttgaccc atttccagct
    24361 gttgttccgg cgttaacgca cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg
    24421 ataatgtcag tgggagctct ggtagttgcg tcatggctgg tgccagataa gaagggaatc
    24481 tccgagatgc cgccgcaggc tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc
    24541 gcagttttaa ggcttctcgg acgaaccgag catgctcacc aaccgcggag cgccacattc
    24601 ttgtggtatg aaatatcggc tcaggggact ggcccgcttg cgaacatctc agagaaattt
    24661 tgtcttcacg gttactctac cacagtaaac cgaaaagtgt cattcaaagt tttggttagt
    24721 tttttcggtg atgcgacctt gttcaagtaa cgcttgttct atggtctgct gcagcatccg
    24781 aatacgctgt tccatacttg ccgcgtagtc acgagtcttt gctttttctt gcgctaactc
    24841 atagctgata ttcaatgcgg caatgaagac caactgttca gtatttgtga ctctagtgcg
    24901 ttctttcaga tcttgcaacc gttggttcag atcgtccgct gcctgattca acgcatccct
    24961 ttggtcaggc gggcagttca cacgcagtga acggccaaaa atttggatat cgacgggttg
    25021 tgcagacatg ccaccttcct gctgattgac tgcgctgcct tcgtctttag acccatggtc
    25081 tgcgaagggg cgacactata gctaccctga tgagaagaga caagcccttt tctggtccac
    25141 cagggcccaa agtggtagca tatcatgaat attcctccct ttgacgacga atgcttatgt
    25201 ctatacagaa cgaaatgcct ggttacaacg aaatgaacca gtatctgaac caacaaggga
    25261 cgggtctgac ccctgccgag atgcatggtt taatcagtgg gatgatatgt ggcggtaacg
    25321 atgacagctc atggctgccg ctacttcacg acctgacgaa cgaaggcatg gctttcggtc
    25381 atgagctggc acaggcactg cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg
    25441 acggcttcct ttttcagctt tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg
    25501 ctgatgcact ggcaggttgg gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga
    25561 agctggacaa agtgaccggc gaaaccggtg aagccatcga cgatctgcgt aacatcgcgc
    25621 agctgggcta cgacgaagac gaagatcagg aagagcttga aatgtcgctt gaagagatca
    25681 tcgaatacgt tcgtgttgcc gcgctgttat gccacgacac ctttactcat ccgcaaccga
    25741 ccgcgccaga agtacaaaaa ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt
    25801 atgagtgaga tatcccggca agagtttcag cgtcgccgtc aggccctggt ggagcaaatg
    25861 caacccggca gcgccgcgct gatttttgct gcaccagaag taacacgtag cgccgacagc
    25921 gaatacccct atcgtcagaa cagtgacttc tggtacttca ccggctttaa cgaaccggaa
    25981 gcggtgctgg tgctgattaa aagcgatgac actcataacc acagcgttct gtttaaccgc
    26041 gttcgcgacc tgacggcgga gatctggttt ggccgtcgct taggccagga tgccgcgcca
    26101 gagaaactgg gcgttgaccg cgcactggca ttcagcgaaa tcaatcagca actttatcaa
    26161 ctacttaacg gcctggatgt ggtttaccat gcccagggcg aatatgcata tgctgatgaa
    26221 atcgtgaaca gtgcgctgga aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg
    26281 gcaacgatga tcgactggcg tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa
    26341 gagattgccg tactccgccg cgcgggagaa atcaccgcca tggcacatac acgggcgatg
    26401 gaaaaatgcc gtccgggaat gttcgagtac catctggaag gcgaaattca ccacgaattt
    26461 aaccgccacg gtgcgcgcta tccttcctat aacaccattg taggcagcgg tgaaaacggc
    26521 tgcattctgc actacaccga aaacgagagt gaactgcgcg acggtgacct ggtgttgatt
    26581 gacgcgggtt gtgaatacaa aggttacgcg ggcgatatta cccgcacctt cccggtcaac
    26641 ggcaaattca cccaggccca gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc
    26701 agcctgcgcc tgtatcgtcc gggaacttcc attctggaag tcactggtga agtggtgcgc
    26761 atcatggtta gcggcctggt aaaactcggc atcctgaaag gtaatgttga tgaactgatc
    26821 gctcagaacg cccatcgtcc tttctttatg catggcctta gccactggtt aggactggat
    26881 gtccatgacg ttggcgttta tggtcaggat cgctcgcgca ttctggaacc ggggatggta
    26941 ctgaccgtag agcctggtct gtatatcgcg ccggatgcgg atgtgccaga acaatatcgc
    27001 ggtatcggca ttcgtattga agacgacatt gtgattaccg aaaccggcaa cgaaaacctc
    27061 accgccagcg tggtgaaaaa gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag
    27121 caatgagcgt aatcatcgtc ggtggcggca tggcgggcgc gacgctggcg ctggctattt
    27181 cccggttaag tcacggggcg ctgccggtac atttgattga agcgactgtg ccagagtcac
    27241 atgctcatcc gggctttgat ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac
    27301 tggcgcgcat cggcgtctgg caatctctgg ctgattgcgc aaccgccatc actaccgtgc
    27361 atgtcagcga tcgtggtcat gctggattcg tcaccctcgc cgcagaagat taccaactgg
    27421 cggcgctggg gcaggttgtc gaattgcata atgtcgggca acggctgttt gcattgctgc
    27481 gaaaagcacc tggcgtaatg ctgcattgcc ccgatcgcgt agctaacgtt gcccgtactc
    27541 agagtcatgt tgaagtgacg ctggagagcg gcgagatgct gacgggccgc gtgctgatag
    27601 ccgctgatgg cacccattca gcgttagcca ccgcgtgcgg cgttgattgg cagcaggagc
    27661 cttacgaaca actggcagtg attgccaacg ttgctacttc tgttgcgcac gaaggccgcg
    27721 cttttgaacg cttcacgcaa catggcccgc tggcgatgtt gccgatgtct gacggacgct
    27781 gttcgctggt ctggtgtcat ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg
    27841 agaagttttg ccgtgaactc cagtctgcct ttggctggcg actggggcaa attacccacg
    27901 ccggtaaacg cagtgcttat ccgctggcat taacccgcgc cgccagaccg attacccatc
    27961 gcactgtgct ggtgggcaat gcggcgcaaa ctctgcaccc gattgccggg caagggttta
    28021 acctcggtat gcgagatgtc atgagccttg cggaaaccct gactcaggcg catgagcgcg
    28081 gagaagacat cggtgattac ggcatattgt gccgctatca gcagcgtcga cagagcgatc
    28141 gcgaagcaac catcggcgtc acggacagcc tggtacatct ttttgccaac cgttgggcac
    28201 cgctggttgt cgggcgcaac atcggactga tgacgatgga attattcacc ccggcacgcg
    28261 atgtgctggc gcagcgcacc ctcggttggg tggcgcgttg aaggcggttt taaggaataa
    28321 acaatgcaaa gtgttgatgt agccattgtt ggcggcggca tggtggggct ggcggttgcc
    28381 tgcggcttac aggggagcgg cttacgcgtt gccgtactgg agcagcgcgt accggaacct
    28441 ctggcggtag atgcactacc acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa
    28501 ttactcaccc gtcttggcgt ctggcaggac attctcgctc gtagggccag ttgttatcac
    28561 ggcatggaag tgtgggacaa agacagtttt ggtcacatct cgtttgacga tcaaagcatg
    28621 ggctatagcc atcttgggca catcgttgaa aactcagtga ttcactacgc gctgtggaac
    28681 aaagcgcagc agtcgtcaga tattactctg ttggccccca cagaattaca gcaggtcgcc
    28741 tggggagaaa atgaaacctt cctgacgctg aaagatggca gcatgttaac ggcgcgtctg
    28801 gtgattggcg cggacggtgc taattcctgg ttgcgcaaca aagcagatat tccgctgact
    28861 ttctgggatt atcagcatca cgcgctggta gcgaccattc gcacggaaga accgcatgat
    28921 gcggtggcgc ggcaggtttt ccatggcgaa ggcattctgg cctttttacc gcttagcgat
    28981 ccgcatcttt gctcgattgt ctggtcactg tcgccagagg aagcgcagcg gatgcagcag
    29041 gcaagtgaag acgaatttaa tcgcgcgtta aatatcgctt ttgataatcg ccttggctta
    29101 tgcaaggttg agagcgcgcg tcaggtgttc ccgctgacgg ggcgttatgc gcgccagttt
    29161 gccgcacacc gtctggcgct ggtgggcgac gccgcgcata ccattcaccc actggcgggg
    29221 cagggcgtta atctcggatt tatggacgcc gcagagctga ttgctgaact gaaacggttg
    29281 catcgtcagg gtaaagacat cgggcagtac atttacctgc gccgctatga acgtagccgc
    29341 aagcacagcg cggcgctgat gctggctggt atgcagggtt ttcgcgatct cttttccggc
    29401 gctaatccgg cgaaaaaact gctgcgtgat attggtttga aactggccga cacgcttcct
    29461 ggcgttaagc cgcaacttat ccgccaggca atgggattaa acgatttgcc tgaatggctg
    29521 cgttaaaaat ttctcctctg ttgtttattt gatacccatc acactttcat ctcccggttt
    29581 tttcgccggg agattttcct catttgaaat aaactaattt cacctccgtt ttcgcattat
    29641 attttctaat accatgattt tttgatttag tgttttttga cattttttta gctcttaata
    29701 ttgtcttatt caaattgact ttctcatcac atcatctttg tatagaaatt ggtgtatttt
    29761 ttggtttttt attctgtcgc gatttttgca ttttttaacc ataagctaat gtgatgatca
    29821 attttacctt atggttaaca gtctgtttcg gtggtaagtt caggcaaaag agaacgattg
    29881 cgttggggac cgggagtggc tccgatgctg ggttttgtgg tgataatttc accatgaaaa
    29941 agttgtcagc cccgcttatt caatgaggac aagatggcac aacagactcc tttgtacgaa
    30001 caacacacgc tttgcggcgc tcgcatggtg gatttccacg gctggatgat gccgctgcat
    30061 tacggttcgc aaatcgacga acatcatgcg gtacgtaccg atgccggaat gtttgatgtg
    30121 tcgcatatga ccatcgtcga tctccgcggc agccgcaccc gggagtttct gcgttatctg
    30181 ctggcgaacg atgtggcgaa gctcaccaaa agcggcaaag ccctttactc ggggatgttg
    30241 aatgcctctg gcggtgtgat agatgatctc atcgtctact actttactga agatttcttc
    30301 cgcctcgttg ttaactccgc cacccgcgaa aaagacctct cctggattac ccaacacgct
    30361 gaacctttcg gcatcgaaat taccgttcgt gatgaccttt ccatgattgc cgtgcaaggg
    30421 ccgaatgctc aggcaaaagc tgccacactg tttaatgatg ctcagcgtca ggcggtggaa
    30481 gggatgaaac cgttctttgg cgtgcaggtg ggcgatctgt ttattgccac cactggttat
    30541 accggtgaag cgggctatga aattgcgctg cccaatgaaa aagcggccga tttctggcgt
    30601 gcgctggtgg aagcgggcgt taagccctgc ggtctggggg cgcgtgacac gctgcgtctg
    30661 gaagcgggca tgaatcttta tggtcaggag atggacgaaa ctatttctcc tttagccgcc
    30721 aacatgggct ggactatcgc ctgggaaccg acagatcgtg actttatcgg tcgtgaagcc
    30781 ctggaagtgc agcgtgagca tggtacagaa aaactggttg gtctggtgat gaccgaaaaa
    30841 ggcgtgctgc gtaatgaact gccggtacgc tttaccgatg cgcagggcaa ccagcatgaa
    30901 ggcattatca ccagcggtac tttctccccg acgctgggtt acagcattgc gctggcgcgc
    30961 gtgccggaag gtattggcga aacggcgatt gtgcaaattc gcaaccgtga aatgccggtt
    31021 aaagtgacaa aacctgtttt tgtgcgtaac ggcaaagccg tcgcgtgatt tacttttttg
    31081 gagattgatt gatgagcaac gtaccagcag aactgaaata cagcaaagaa cacgaatggc
    31141 tgcgtaaaga agccgacggc acttacaccg ttggtattac cgaacatgct caggagctgt
    31201 taggcgatat ggtgtttgtt gacctgccgg aagtgggcgc aacggttagc gcgggcgatg
    31261 actgcgcggt tgccgaatcg gtaaaagcgg cgtcagacat ttatgcgcca gtaagcggtg
    31321 aaatcgtggc ggtaaacgac gcactgagcg attccccgga actggtgaac agcgaaccgt
    31381 atgcaggcgg ctggatcttt aaaatcaaag ccagcgatga aagcgaactg gaatcactgc
    31441 tggatgcgac cgcatacgaa gcattgttag aagatgagta acggctttat tcctcttctg
    31501 cgggagagga tcagggtgag gaaaatttat gcctcaccct cactctcttc gtaaggagag
    31561 aggttcacaa ttcactgcac gtttcaggaa ccatcgctca tgacacagac gttaagccag
    31621 cttgaaaaca gcggcgcttt tattgaacgc catatcggac cggacgccgc gcaacagcaa
    31681 gaaatgctga atgccgttgg tgcacaatcg ttaaacgcgc tgaccggcca gattgtgccg
    31741 aaagatattc agcttgcgac accaccgcag gttggcgcac cggcgaccga atacgccgca
    31801 ctggcagaac tcaaggctat tgccagtcgc aataaacgct tcacgtctta catcggcatg
    31861 ggttacaccg ccgtgcagct accgccggtt atcctgcgta acatgctgga aaatccgggc
    31921 tggtataccg cgtacactcc gtatcagcct gaagtctccc agggccgcct tgaagcactg
    31981 ctcaacttcc agcaggtaac gctggatttg actggactgg atatggcctc tgcttctctt
    32041 ctggacgagg ccaccgctgc cgccgaagca atggcgatgg cgaaacgcgt cagcaaactg
    32101 aaaaatgcca accgcttctt cgtggcttcc aatgtgcatc cgcaaacgct ggatgtggtc
    32161 cgtactcgtg ctgaaacctt tggttttgaa gtgattgtcg atgacgcgca aaaagtgctc
    32221 gaccatcagg acgtcttcgg cgtgctgtta cagcaggtag gcactaccgg tgaaattcac
    32281 gactacactg cgcttattag cgaactgaaa tcacgcaaaa ttgtggtcag cgttgccgcc
    32341 gatattatgg cgctggtgct gttaactgcg ccgggtaaac agggcgcgga tattgttttt
    32401 ggttcggcgc aacgcttcgg cgtgccgatg ggctacggtg gcccacacgc ggcattcttt
    32461 gcggcgaaag atgaatacaa acgctcaatg ccgggccgta ttatcggtgt atcgaaagat
    32521 gcagctggca ataccgcgct gcgcatggcg atgcagactc gcgagcaaca tattcgtcgt
    32581 gagaaagcga actccaacat ttgtacttcc caggtactgc tggcaaacat cgccagcctg
    32641 tatgccgttt atcacggccc ggttggcctg aaacgtatcg ctaaccgcat tcaccgtctg
    32701 accgatattc tggcggcggg cctgcaacaa aaaggtctga aactgcgcca tgcgcactat
    32761 ttcgacacct tgtgtgtgga agtggccgac aaagcgggcg tactgacgcg tgccgaagcg
    32821 gctgaaatca acctgcgtag cgatatcctg aacgccgtgg ggatcaccct tgatgaaacc
    32881 accacgcgtg aaaacgtaat gcagcttttc agtgtgctgc tgggcgataa ccacgggctg
    32941 gagatcgaca cgctggacaa agacgtggct cacgacagcc gctctatcca gcctgcgatg
    33001 ctgcgcgacg acgaaatcct cacccatccg gtgtttaatc gctatcacag cgaaaccgaa
    33061 atgatgcgct atatgcactc gctggagcgt aaagatctgg cgctgaatca ggcgatgatc
    33121 ccgctgggtt cctgcaccat gaaactgaac gccgccgccg agatgatccc aatcacttgg
    33181 acggaatttg ccgaactgca cccgttttgc ccgccggaac aggctgaagg ttatcagcag
    33241 atgattgcgc agctggctga ctggctggtg aaactgaccg gttacgacgc cgtttgtatg
    33301 cagccgaact ccggcgcaca gggcgaatac gcgggcctgc tggcgattcg tcattatcat
    33361 gaaagccgca acgaagggca tcgcgatatc tgcctgatcc cggcttctgc gcacggaact
    33421 aaccccgctt ctgcacatat ggcaggaatg caggtggtgg ttgtggcgtg tgataaaaac
    33481 ggcaacatcg atctgactga tctgcgcgcg aaagcggaac aggcgggcga taacctctcc
    33541 tgtatcatgg tgacttatcc ttctacccac ggcgtgtatg aagaaacgat ccgtgaagtg
    33601 tgtgaagtcg tgcatcagtt cggcggtcag gtttaccttg atggcgcgaa catgaacgcc
    33661 caggttggca tcacctcgcc gggctttatt ggcgcggacg tttcgcacct caacctgcat
    33721 aaaactttct gcattccgca cggcggtggt ggtccgggta tgggaccgat cggtgtgaaa
    33781 gcgcatctgg caccgtttgt accgggtcat agcgtggtgc aaatcgaagg catgttaacc
    33841 cgtcagggcg cagtttctgc ggcaccgttc ggtagcgcct ctatcctgcc aatcagctgg
    33901 atgtacatcc gcatgatggg cgcagaaggg ctgaaaaaag caagccaggt ggcaatcctc
    33961 aacgccaact atattgccag ccgcctgcag gatgccttcc cggtgctgta taccggtcgc
    34021 gacggtcgcg tggcgcacga atgtattctc gatattcgcc cgctgaaaga agaaaccggc
    34081 atcagcgagc tggacattgc caagcgcctg atcgactacg gtttccacgc gccgacgatg
    34141 tcgttcccgg tggcgggtac gctgatggtt gaaccgactg aatctgaaag caaagtggaa
    34201 ctggatcgct ttatcgacgc gatgctggct atccgcgcag aaattgacca ggtgaaagcc
    34261 ggtgtctggc cgctggaaga taacccgctg gtgaacgcgc cgcacattca gagcgaactg
    34321 gtcgccgagt gggcgcatcc gtacagccgt gaagttgcgg tattcccggc aggcgtggca
    34381 gacaaatact ggccgacggt gaaacgtctg gatgatgttt acggcgaccg taacctgttc
    34441 tgctcctgcg taccgattag cgaataccag taattcactg attcgagtat tttctaaagg
    34501 cgcttcggcg cctttttagt cagatgacaa agtacaaaag tgctcagaca gtcccctcgc
    34561 ccctttgggg agagggttag ggtgagggga acaggccggc actggcgcga atatttaccc
    34621 tcaccccgac cctctccctg aaagggcgag ggggaaaagc gtgccaacat tgaagattga
    34681 gccagtttgt tagcaatctc aaagatacgt caacgaatta atttttctcg gaaaaacaaa
    34741 tggctatagc acttgtgacc ggtggcagtc gcggcatcgg gcgggcaact gcattactgt
    34801 tggcgcaaga agggtatacg gtggcggtta attatcagca aaacctccac gcggcgcagg
    34861 aagtgatgaa cttaataatg caagccggtg gcaaagcatt cgtgctccag gcggatatca
    34921 gcgacgaaaa ccaggtcgtt gcgatgttta cagcaatcga tcagcacgat gaaccgctag
    34981 cagcgctggt caataacgcc gggatcttgt ttacccagtg caccgttgaa aaccttaccg
    35041 cagagcgaat caaccgagta ctttccacca acgtgacggg atattttctc tgctgccgcg
    35101 aggcggtaaa acgcatggcg cttaaaaatg gtggcagtgg cggcgctatc gtcaatgtct
    35161 cttcggtggc ctcacggttg ggttcgccag gggaatatgt tgattacgcg gcatcgaaag
    35221 gggcgattga ttcgttaacc accggactat cgctggaagt cgccgcgcag gggatccgcg
    35281 ttaactgcgt gcggccaggg tttatttata ccgaaatgca cgccagcggc ggcgagcctg
    35341 gacgcgtcga tcgcgttaag tcgaacatcc ccatgcagcg tggtggacag gcagaagagg
    35401 tcgcgcaggc cattgtctgg ctactaagtg ataaagcctc ttacgtcacg ggaagtttta
    35461 tcgatttggc gggcgggaaa taaaacaggg aagttgtctg accggatgca acaagtattg
    35521 catccggtac ttcatcgact taaagcttct cgccgttgct ggcaatcacc tctttgtacc
    35581 agttaaagct cttcttacgt gaacgcgaca tatcgccagt accgtcgtca tgtttattca
    35641 catagataaa gccgtagcgt ttgctgtact gcccggtagt gaatgacacg cagtcgatgc
    35701 agccccacgg tgtgtagccc atcagatcca caccatcgta agtcactgct tttttcatct
    35761 cttcaatatg ggcgcgcagg tagtcaatgc ggtagtcgtc gttgatgctg ccatcttctt
    35821 ccactttgtc gtaagcgcca aaaccgtttt cgacaataaa cagcggcctc tgataacgct
    35881 catacagttc gcaaagggca tagcgcagac ctactggatc aatctgccag ccccagtcag
    35941 acgctttaac atacgggttc ggtacgctgc cttcaaaacc agagatcgca tcgccggtgc
    36001 cgccttcggc cttcactgcg ttggtcatgt aatagctgaa accaagataa tcgcaggtgc
    36061 cttcgcgcag tacatcgaga tcgccgtctt ccattttgat attaaatccg cgacgttccc
    36121 actcgttcaa tacataggac gggtaatagc cgcatagctg cacatcggta aagacgtagc
    36181 gttcgcgcat cgactcctga gcgaacatca catcgtccgg gttacatgag tagggataga
    36241 gcggcaccat cgccagcata cagccgactt tcatctccgg gttaatgcga cgcgcggctt
    36301 tcactgccag ggcgctggcg acaaactggt gatgcagcac ctggtacatc gtctcttccg
    36361 ggttgtcatg ctcggtatac accacgccgg agcagcagta accaaacagc ggcgcacgcc
    36421 agttacgctg gttgtttatt tcgttgaagg tcatccagta tttgacttta tgcttgtagc
    36481 gttcgaatac cacttccgcg aaacggacaa agaaatcaac gaatttacgg ttggtccagc
    36541 taccgtattg ctgcaccaga tgcagcggca tttcaaagtg ggagagggtt atcaccggtt
    36601 cgatgttgta tttcagcagt tcatcgaaca gagagtcgta aaacttcagc ccttcttcgt
    36661 ttggctgagc ttcatcacct tttggaaaaa tacgcgtcca ggcgatggaa gtccgaaaac
    36721 atttgaagcc catttcggca aatagcttga tgtcttcctt gtagtgacca taaaaatcaa
    36781 cggcttcatg gtttggatag tattttcctg gcaagacttc tttggtgatt tcgcgcggca
    36841 cgccgtgtgc gccacaggtc agaacgtcac aaatgctcgg tcctttgccg cctttgttcc
    36901 agccgccttc gacctgatga gcggcaacgg cgccacccca taagaaatct ttcggtaagg
    36961 tgagtttttt cactatcata ggctccagat cagtcaattc ttacaggaaa tagtaccatg
    37021 cagccaaacg acatcacttt tttcaacgtt tccaggatga cattctggct gggcgtaaaa
    37081 ccatcaccat ccgcgacgag tctgaatcgc acttcaaaac gggtgatgtg cttcgtgtcg
    37141 gacgttttga agatgacggt tatttttgca cgattgaagt caccgcaacc tcaaccgtaa
    37201 cgctggatac actgacggaa aaacatgcag agcaggaaaa tatgaccctg cctgaactga
    37261 aaaaggtcat tgctgacatc tatcccggtc agacacaatt ttatatgatt gaatttaaat
    37321 gtctttaaat tgaactcacg cctgttagtt gaaatttcgc atttaatttc atgattttta
    37381 ggagattagt ctcaaatcag aatttatttt agctaacagg tgttcactgg aactattctc
    37441 agttacgctg gagaggtata catgtgcgtg tacgtttctc tggagtaagt tatggttcag
    37501 aagcccctca ttaagcaggg atattcgctg gcagaggaaa ttgccaacag cgtcagtcac
    37561 ggcattgggc tggtgtttgg tatcgttggg ctggtgttgc tactggttca ggcggtggat
    37621 ctcaatgcca gcgccacagc gataaccagt tacagcctct atggcggcag tatgatcctg
    37681 ctgttcctcg cttcgacgct ctatcacgcc attcctcatc aacgggcaaa aatgtggctg
    37741 aagaaatttg accattgcgc tatttacctg ttgattgccg gaacctacac gccgtttttg
    37801 ctggtggggc tggattctcc gttagcgcgc gggttgatga ttgttatctg gagcctagca
    37861 ttgctgggga ttctgttcaa actgaccatc gcgcaccgat tcaaaatttt atctctggtg
    37921 acttatctgg cgatgggctg gctgtcgctg gtggtaattt atgaaatggc agttaagctc
    37981 gcggcgggca gcgttacctt actggcggta ggcggcgtgg tttattcgct cggggtgatt
    38041 ttctacgtct gcaaacgcat tccatacaac catgccatct ggcacggctt cgtgctcggc
    38101 ggtagtgtgt gccactttct ggcgatctat ttgtatattg ggcaggcgta atgggtatgc
    38161 ctttgtaggc ctgataagac gcgtcagcgt cgcatcaggc atcggagcgc aaccgccgga
    38221 tgcggcgtga acgccttatc cgatctacgg gcctcgtccc tgttgtgata aaggcatcaa
    38281 atcccagaca tgataagacg cgtcagcgtc gcatcaggcg ataaatgcct gatgcgatac
    38341 agagatttac tcttcgagcg aatacggcag cggctcgata tgcagtgtat tcgcatcgtc
    38401 gcgtacgcgg aagatgctgt ccggttccat atcgttattc atgacgacct gtaccacgac
    38461 ctgaccatct tccagtttta ccgcagccag caccgtaccg gtacgacgcc agttctcgcc
    38521 cattttcagc tctaagtctt caccagcttc cggcagtcgg ctggcactac ctgccagcaa
    38581 ccagagcgcg cgtttgttgg caccacggaa ttttgctcgc gccaccatct cttgtccggt
    38641 ataacagcct ttcttaaagc tgataccgcc cagcgcctgg agattggtcg cctgtgggat
    38701 aaactgcccg ctgttggcgg catcaatcac cgggaaacca gcttcaatgt ttaatgccag
    38761 ccactgttgg ctattgttca gttccgcttc accgcgcagt ttatcggtca gcatattagc
    38821 agtagcttca tcggttacga tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt
    38881 ggtcgcgcct tctttgacta cctgtttttc tttcgaaggc agttcgctaa agagatttgc
    38941 cagcgcggcg cgcgcctgaa aaccggcaac accaagcagc acacgctcgt cgtctggcgc
    39001 gatggtcact ttagagaata ccgcatattt tttcagttca gtcagctgcg gttcacgcac
    39061 gctgcgccgt tcaatccatg caaagccatc gccgtcgcgg aacagacgta aattgctcca
    39121 cattttacct ttggcgtcgc aatgggcggc gagcaggtgc tgatcttctg ccatctggct
    39181 gacatctgct gtcacctgac cctgcatata tttttcgctg tccgcgccag taatggtggc
    39241 aagcgcccag tcatcaagcg tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg
    39301 acgtggagga aaaggtgtaa aagccataat agagtcctga gttgcttaac ggcgtattaa
    39361 tgactaatgg taaaagagct atgaggcaat gcaagtgctt tcactacgcg atctgtgcct
    39421 tctgcgaggc gactgccaaa aaaatgaagc ccttgaattg ttgtgaatta atttcgcgat
    39481 cgcgatggtg tttactgata ttcccgtaaa agaagtcgaa aaacacgtta caatagccgg
    39541 gtactggatt ttcgtgagac aggaagaaga tggacattaa caacaaagcc cgaattcatt
    39601 gggcatgccg ccgtggtatg cgcgaactcg atatttcaat catgtcgttt ttcgaacatg
    39661 agtacgacag cttaagcgat gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg
    39721 atccggacct gtttaactgg ctgatgaatc acggtaaacc ggccgatgca gaactggaaa
    39781 taatggtccg actcatccag acacggaacc gggaacgtgg tcctgtggca atctgatttg
    39841 cgcgtctcct ggcgcgcaca gtggctttcc ttgctgattc atgggctggt tgccgctgtt
    39901 attttactca tgccctggcc gctcagttac accccgttat ggatggtgtt actttcgctg
    39961 gtggtatttg attgcgttcg cagccagcgg tgtattaatg ctcgccaggg ggaaattcgc
    40021 ttgttgatgg acgggcgttt gcgttggcaa gggcaggagt ggagcatcgt caaagcaccg
    40081 tggatgatta agagcggcat gatgctgcgt ttacgttctg atggcggtaa acggcaacat
    40141 ttatggctgg cagccgacag catggacgaa gccgaatggc gggatttacg gcggattttg
    40201 ttgcaacaag agacgcaaag ataaagatga cggcaaagca tatgcgctaa gccgtcagtg
    40261 agatcaggcg taatgctctg ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg
    40321 ctcgtcgcta aggtcatact ggttagtttc atccagcgcc agacccacga acagttgccc
    40381 gtcagcaatc accggtttcg ggctggtaaa ttcatatcct tccgttggcc agtagccgac
    40441 gaacttcacg cctttggtcg agagtttgtc atgcagcata ccgagcgcat cgaggaacca
    40501 ctcgccgtat cccagttgat cgccaagccc atacagcgca ataattttac cttcaaggtt
    40561 caggtcgtcg agctgatccc agacggcttc ccagtcttcc tggatttcac caaaatccca
    40621 ggtcgggata cccagaatga gcacatcgta ctgctccatt aatttcgggg agtcgtcctt
    40681 gaggttatgt aaggtcacca gttctgggcc gataatatcg cggatttttt ctgccgccat
    40741 ttcggtgtaa caggtgctgg aaccgtaaaa aagacccata ttcataacgt aagcttctcg
    40801 attctgctgt ggcttttgca gctagtgtat cagaatcgct ttagtcacgg cacaataagg
    40861 cataatggat caaatgagtg accataaggg gcgcaagtga aacaggagct ggcacgcatc
    40921 gagcagtttc ttgatgctct gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct
    40981 taccgtcgcg atctgtcaat gatggtggag tggttgcatc accgcgggtt gacgctggcg
    41041 acggcgcaaa gtgacgattt gcaggcatta ctggcagaac ggctggaggg cgggtataaa
    41101 gccaccagct cagcgcgttt gctgagtgca gtgcgccgat tgttccagta tctttatcgc
    41161 gaaaagtttc gtgaagacga tcccagtgcg catctcgctt caccgaaatt gccccagcgt
    41221 ttgccaaaag atttaagcga agcgcaggtc gaacgtttat tacaggcgcc attaattgat
    41281 cagccactgg agctacgcga taaagccatg cttgaagtgt tgtatgctac cgggctgcgt
    41341 gtctctgaac tggtcgggct gacaatgagt gatatcagcc tgcgtcaggg cgtagtgcgg
    41401 gtcattggta aaggcaacaa agagcgcctg gtgccgttag gggaagaggc ggtttactgg
    41461 ctggaaacct atctggaaca tgggcgtccg tggctgctga atggtgtgtc aattgacgtg
    41521 ttgtttccca gccagcgtgc gcagcagatg acgcgacaga ccttctggca ccgcattaaa
    41581 cattatgccg tgctggcggg tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac
    41641 gcttttgcca ctcatttatt aaatcatggt gcggatttac gcgtggtgca gatgctactg
    41701 ggccacagcg atctttccac cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg
    41761 caacttcatc aacagcatca cccgcgggcg tgatgtctga aaagaacggg aagatttatg
    41821 aagaaaggtt ttatgttgtt tactttgtta gcggcgtttt caggctttgc tcaggctgat
    41881 gacgcggcaa ttcaacaaac gttagccaaa atgggcatca aaagcagcga tattcagccc
    41941 gcgcccgtag ctggcatgaa gacagttctg actaacagcg gtgtgttgta catcaccgat
    42001 gatggtaaac atatcattca ggggccaatg tatgacgtta gtggcacggc tccggtcaat
    42061 gtcaccaata agatgctgtt aaagcagttg aatgcgcttg aaaaagagat gatcgtttat
    42121 aaagcgccgc aggaaaaaca cgtcatcacc gtgtttactg atattacctg tggttactgc
    42181 cacaaactgc atgagcaaat ggcagactat aacgcgctgg ggatcaccgt gcgttatctt
    42241 gctttcccgc gccaggggct ggacagcgat gcagagaaag aaatgaaagc tatctggtgt
    42301 gcgaaagata aaaacaaagc gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc
    42361 agctgcgacg tggatattgc cgaccattac gcacttggcg tccagcttgg cgttagcggt
    42421 actccggcag ttgtgctgag caatggcaca cttgttccgg gttaccagcc gccgaaagac
    42481 atgaaagaat tcctcgacga acaccaaaaa atgaccagcg gtaaataatt cgcgtgaaac
    42541 aacagataca acttcgtcgc cgtgaagtcg atgaaacggc agacttgccc gctgaattgc
    42601 ctcccttgct gcgccgttta tacgccagcc ggggagtacg cagtgcgcaa gaactggaac
    42661 gcagtgttaa aggtatgttg ccctggcagc aactaagcgg tgtcgaaaag gccgttgaga
    42721 tcctttacaa cgcttttcgc gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg
    42781 acggcgcgac cagcacggct ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata
    42841 tcgactacct ggtaccaaac cgtttcgaag acggttacgg cttaagcccg gaagtggtcg
    42901 atcaggccca tgcccgtggc gcgcagttaa ttgtcacggt ggataacggt atttcctccc
    42961 atgcgggagt tgagcacgct cgctcgttgg gcatcccggt tattgttacc gatcaccatt
    43021 tgccgggcga cacattaccc gcagcggaag cgatcattaa ccctaacttg cgcgactgta
    43081 atttcccgtc gaaatcactg gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc
    43141 gcaccttttt gcgcgatcag ggctggtttg atgagcgcgg catcgcaatt cctaacctgg
    43201 cagaactgct ggatctggta gcactgggga cagtagcgga cgtcgtgccg ctggacgcta
    43261 ataatcgcat tctgacctgg caggggatga gtcgcatccg tgccggaaag tgccgtccag
    43321 ggattaaagc gctgctggaa gtggcaaatc gcgatgcaca aaaactagcc gccagcgatt
    43381 taggttttgc gctgggaccg cgtctcaatg ctgccgggcg actggatgat atgtccgttg
    43441 gtgtggcgct gttgctgtgc gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg
    43501 atgcgctaaa ccagacgcga aaagagatcg aacaaggaat gcaggttgaa gccctgaccc
    43561 tgtgcgagaa actggagcgc agccgtgaca cgctacccgg cgggctggca atgtatcacc
    43621 ccgaatggca tcagggcgtt gtcggtattc tggcttcgcg tatcaaagag cgttttcacc
    43681 gtccggttat cgcctttgcg ccagcaggtg acggtacgct gaaaggttcc ggtcgctcca
    43741 ttcaggggct gcatatgcgt gatgcgctgg agcgattaga cacactctac cccggcatga
    43801 tgctcaagtt tggcggtcat gcgatggcgg cgggtttgtc gctggaagag gataaattcg
    43861 aactctttca acaacggttt ggcgaactgg ttactgagtg gctggcccct tcgctattgc
    43921 aaggcgaagt ggtgtcagac ggtccgttaa gcccggccga aatgaccatg gaagtggcgc
    43981 agctgctgcg cgatgctggc ccgtgggggc agatgttccc ggagccgctg tttgacggtc
    44041 atttccgtct gctgcaacag cggctggtgg gcgaacgtca tttgaaggtg atggtcgaac
    44101 cggtcggcgg cggtccactg ctggatggta ttgcttttaa tgtcgatacc gctctctggc
    44161 cggataacgg cgtgcgcgaa gtgcaactgg cttataagct cgatatcaac gagtttcgcg
    44221 gcaaccgcag cctgcaaatt atcatcgaca atatctggcc aatttagcgt catcttctct
    44281 ataaaaaaga gcgtggattg ggtacaatcc cgctcttatc accgcatttt gactagctca
    44341 ataaaagaaa tcagaccatg tttgaaatta atccggtaaa taatcgcatt caggacctca
    44401 cggaacgctc cgacgttctt agggggtatc tttgactacg acgccaagaa agagcgtctg
    44461 gaagaagtaa acgccgagct ggaacagccg gatgtctgga acgaacccga acgcgcacag
    44521 gcgctgggta aagagcgttc ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa
    44581 caggggctgg aagatgtttc tggtctgctg gaactggctg tagaagctga cgacgaagaa
    44641 acctttaacg aagccgttgc tgaactcgac gccctggaag aaaaactggc gcagcttgag
    44701 ttccgccgta tgttctctgg cgaatatgac agcgccgact gttacctcga tattcaggcg
    44761 gggtctggcg gtacggaagc acaggactgg gcgagcatgc ttgagcgtat gtatctgcgc
    44821 tgggcagaat cgcgtggttt caaaactgaa atcatcgaag agtcggaagg tgaagtggcg
    44881 ggtattaaat ccgtgacgat caaaatctcc ggcgattacg cttacggctg gctgcgtaca
    44941 gaaactggcg ttcaccgcct ggtgcgtaaa agcccgtttg actccggcgg tcgtcgccac
    45001 acgtcgttca gctctgcgtt tgtttatccg gaagttgatg atgatattga tatcgaaatc
    45061 aacccggcgg atctgcgcat tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt
    45121 aaccgtaccg aatctgcggt gcgtattacc cacatcccga ccgggatcgt gacccagtgc
    45181 cagaacgacc gttcccagca caagaacaaa gatcaggcca tgaagcagat gaaagcgaag
    45241 ctttatgaac tggagatgca gaagaaaaat gccgagaaac aggcgatgga agataacaaa
    45301 tctgacatcg gctggggcag ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa
    45361 gatctgcgca ccggggtaga aacccgcaac acgcaggccg tgctggacgg cagcctggat
    45421 caatttatcg aagcaagttt gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac
    45481 gcacagggcg ctgacgcggt agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag
    45541 ctggcgaacc tgcgtgagca gggaattgcc ttcccgaacg atttccgtcg cgatcatacc
    45601 tctgaccaat tgcacgcaga attcgacggt aaagagaacg aagaactgga agcgctgaac
    45661 atcgaagtcg ccgttgctgg ccgcatgatg acccgccgta ttatgggtaa agcgtctttc
    45721 gttaccctgc aggatgtggg tggccgcatt cagctgtacg ttgcccgtga cgatctgccg
    45781 gaaggcgttt acaacgagca gttcaaaaaa tgggacctcg gcgacatcct cggcgcgaaa
    45841 ggtaaactgt tcaaaaccaa aaccggcgaa ctgtctatcc actgtaccga gctgcgtctg
    45901 ctgaccaaag cactgcgtcc gctgccggat aaattccacg gcttgcagga tcaggaagcg
    45961 cgctatcgtc agcgttatct ggatctcatc tctaacgatg aatcccgtaa cacctttaaa
    46021 gtacgctcgc agatcctctc tggtattcgc cagttcatgg tgaaccgcgg ctttatggaa
    46081 gttgaaacgc cgatgatgca ggtgatccca ggcggtgccg ctgcgcgtcc gtttatcacc
    46141 caccataacg cgctggatct cgacatgtac ctgcgtatcg cgccggaact gtacctcaag
    46201 cgtctggtgg tcggtggctt cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa
    46261 ggtatttccg tacgtcataa cccagagttc accatgatgg aactctacat ggcttacgca
    46321 gattacaaag atctgatcga gctgaccgaa tcgctgttcc gtactctggc acaggatatt
    46381 ctcggtaaga cggaagtgac ctacggcgac gtgacgctgg acttcggtaa accgttcgaa
    46441 aaactgacca tgcgtgaagc gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg
    46501 gataacttcg actctgcgaa agcgattgct gaatctatcg gtatccacgt tgagaagagc
    46561 tggggtctgg gccgtatcgt taccgagatc ttcgaagaag tggcagaagc acatctgatt
    46621 cagccgacct tcattactga atatccggca gaagtttctc cgctggcgcg tcgtaacgac
    46681 attaacccgg aaatcacaga ccgctttgag ttcttcatcg gtggtcgtga aatcggtaac
    46741 ggctttagcg agctgaatga cgcggaagat caggcgcaac gcttcctgga tcaggttgcc
    46801 gcgaaagacg caggtgacga cgaagcgatg ttctacgatg aagattacgt taccgcactg
    46861 gaacatggct taccgccgac agcaggtctg gggattggca tcgaccgtat ggtaatgctg
    46921 ttcaccaaca gccataccat ccgcgacgtt attctgttcc cggcgatgcg tccggtgaaa
    46981 taagcattac gttatgctca caaccccggc aaatgtcggg gttttttatt taagctgggt
    47041 aaatgcagat aatcgtattc tggcttcgcg atttgtcgcc tgcatcacca tccacggact
    47101 gaacgcccac ggcgtggcat caataccgcg taatacatca gctaaatcac accattgata
    47161 atccatcact tcatcatcgt tgatctgtaa cgcactggtg gtgcgtgcgg caaataccgg
    47221 acacacttca ttttccacaa tgcctctcgg atcggtggcg cggtagcgaa agtcaggata
    47281 gatagattca ggaggcgtaa tttccacgcc aagctcataa cggcaacggc ggatcaccgc
    47341 gtcttcgctg ctttctccca gttgtgggtg cccacaaacc gagttagtcc acacgccagg
    47401 ccacgctttt ttgctcagtg cgcggcgggt aactaataat tgccccttgg cattaaacag
    47461 ccaactggag aacgcgagat gtaagagggt gtctgccgtg tgtgcggcat acttttccag
    47521 cgtacccgtg ggaactccct gtgcattcaa taaaatgacg tgttccgttt gcataatttc
    47581 tcacatgtaa ttctgatcac tcatcattat agataattga agagtgaaat gattaaccaa
    47641 gagagcgttt atcgacaacc gcccgttttg agcggatgcc gacaggtaaa aaccatttaa
    47701 aaaagtagca tcatatattt gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg
    47761 acaggaatag agagagtaaa aagcaacaac agactgaata tagcgcgcat taaaaaattc
    47821 atattattct ccatgctcat ttttcttcag cttgcgcatc agaagccaca tacgtactgt
    47881 cctgacttct aaatctttat cagagacgga ttccttcacc aggtcagcat gatgctgata
    47941 gccattaaag aagacgttca gcacaacggc gctaagggtt gcgagcataa tgccgctatg
    48001 tagcagcggt tgcagtacgg ccggtaactt agaaaagaaa tcgtgagaga gcgtcggagt
    48061 catgccaacg ccgagactga tcgccacaat atagaggttg taacggttgg tggtgtagtt
    48121 acagcgcgac agaattcgaa tccctgtcgc cagtaccatg ccgaacatca ctagaccagc
    48181 gccgcccagc acaaattgcg gaatggaggc taccagcacc gccatttttg gcaccatgcc
    48241 aaacaggatt aatataattc ccgaagaaat acacacccag cggctatgaa cgcgcatcac
    48301 gctaaccagg ccaacgtttt gagaaaatga cgtgtggggg aagctgttaa acgtgccgcc
    48361 tatcattgtc cctacgccat cgac
//
LOCUS       DAJTYM010000018        47731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTYM010000018 DAJTYM010000000
VERSION     DAJTYM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO575_001070"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO575_001070"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6664120.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO575_001071"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO575_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6664121.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO575_001072"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO575_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6664122.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO575_001073"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO575_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6664123.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO575_001074"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO575_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6664124.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO575_001075"
     CDS             5377..5835
                     /locus_tag="OO575_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6664125.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO575_001076"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO575_001076"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6664126.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO575_001077"
     tRNA            6887..6971
                     /locus_tag="OO575_001077"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO575_001078"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO575_001078"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO575_001079"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO575_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6664127.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO575_001080"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO575_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6664128.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO575_001081"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO575_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6664129.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO575_001082"
     CDS             complement(10252..13995)
                     /locus_tag="OO575_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6664130.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO575_001083"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO575_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6664131.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO575_001084"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO575_001084"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6664132.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO575_001085"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO575_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6664133.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO575_001086"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO575_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6664134.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO575_001087"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO575_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6664135.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO575_001088"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO575_001088"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6664136.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO575_001089"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO575_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6664137.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO575_001090"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO575_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6664138.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO575_001091"
     CDS             23392..23499
                     /locus_tag="OO575_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6664139.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO575_001092"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO575_001092"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6664140.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO575_001093"
     CDS             complement(24538..25347)
                     /locus_tag="OO575_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6664141.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPPAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO575_001094"
     CDS             complement(25351..25743)
                     /locus_tag="OO575_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6664142.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO575_001095"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO575_001095"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6664143.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO575_001096"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO575_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6664144.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO575_001097"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO575_001097"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6664145.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO575_001098"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO575_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6664146.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO575_001099"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO575_001099"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6664147.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO575_001100"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO575_001100"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6664148.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO575_001101"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO575_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6664149.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO575_001102"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO575_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6664150.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO575_001103"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO575_001103"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6664151.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO575_001104"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO575_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6664152.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO575_001105"
     CDS             36790..39657
                     /locus_tag="OO575_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6664153.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO575_001106"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO575_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6664154.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO575_001107"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO575_001107"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6664155.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO575_001108"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO575_001108"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6664156.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO575_001109"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO575_001109"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6664157.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO575_001110"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO575_001110"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6664158.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO575_001111"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO575_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11985 a        12300 c        11687 g        11759 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg gcgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca g
//
LOCUS       DAJTYM010000019        47522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTYM010000019 DAJTYM010000000
VERSION     DAJTYM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            424..1554
                     /gene="fruB"
                     /locus_tag="OO575_001112"
     CDS             424..1554
                     /gene="fruB"
                     /locus_tag="OO575_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6664159.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            1554..2492
                     /gene="fruK"
                     /locus_tag="OO575_001113"
     CDS             1554..2492
                     /gene="fruK"
                     /locus_tag="OO575_001113"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6664160.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            2509..4200
                     /gene="fruA"
                     /locus_tag="OO575_001114"
     CDS             2509..4200
                     /gene="fruA"
                     /locus_tag="OO575_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6664161.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            4624..5565
                     /locus_tag="OO575_001115"
     CDS             4624..5565
                     /locus_tag="OO575_001115"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6664162.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            5553..6491
                     /gene="psuG"
                     /locus_tag="OO575_001116"
     CDS             5553..6491
                     /gene="psuG"
                     /locus_tag="OO575_001116"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6664163.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            6585..7835
                     /locus_tag="OO575_001117"
     CDS             6585..7835
                     /locus_tag="OO575_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6664164.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7906..8600)
                     /gene="yeiL"
                     /locus_tag="OO575_001118"
                     /pseudo
     CDS             complement(7906..8600)
                     /gene="yeiL"
                     /locus_tag="OO575_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            8731..9672
                     /gene="rihB"
                     /locus_tag="OO575_001119"
     CDS             8731..9672
                     /gene="rihB"
                     /locus_tag="OO575_001119"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6664165.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            9772..11022
                     /locus_tag="OO575_001120"
     CDS             9772..11022
                     /locus_tag="OO575_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6664166.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(11078..12166)
                     /locus_tag="OO575_001121"
     CDS             complement(11078..12166)
                     /locus_tag="OO575_001121"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6664167.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(12169..13026)
                     /gene="nfo"
                     /locus_tag="OO575_001122"
     CDS             complement(12169..13026)
                     /gene="nfo"
                     /locus_tag="OO575_001122"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6664168.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(13100..14149)
                     /locus_tag="OO575_001123"
     CDS             complement(13100..14149)
                     /locus_tag="OO575_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6664169.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            14248..15129
                     /locus_tag="OO575_001124"
     CDS             14248..15129
                     /locus_tag="OO575_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6664170.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            15335..16804
                     /gene="lysP"
                     /locus_tag="OO575_001125"
     CDS             15335..16804
                     /gene="lysP"
                     /locus_tag="OO575_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6664171.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            17096..19087
                     /gene="cirA"
                     /locus_tag="OO575_001126"
                     /pseudo
     CDS             17096..19087
                     /gene="cirA"
                     /locus_tag="OO575_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(19119..19954)
                     /gene="yeiG"
                     /locus_tag="OO575_001127"
                     /pseudo
     CDS             complement(19119..19954)
                     /gene="yeiG"
                     /locus_tag="OO575_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            20212..20880
                     /gene="folE"
                     /locus_tag="OO575_001128"
     CDS             20212..20880
                     /gene="folE"
                     /locus_tag="OO575_001128"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6664172.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20897..21973
                     /locus_tag="OO575_001129"
     CDS             20897..21973
                     /locus_tag="OO575_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6664173.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            22115..23155
                     /gene="galS"
                     /locus_tag="OO575_001130"
     CDS             22115..23155
                     /gene="galS"
                     /locus_tag="OO575_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6664174.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            23435..24433
                     /gene="mglB"
                     /locus_tag="OO575_001131"
     CDS             23435..24433
                     /gene="mglB"
                     /locus_tag="OO575_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6664175.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            24494..26014
                     /gene="mglA"
                     /locus_tag="OO575_001132"
     CDS             24494..26014
                     /gene="mglA"
                     /locus_tag="OO575_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6664176.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            26030..27040
                     /gene="mglC"
                     /locus_tag="OO575_001133"
     CDS             26030..27040
                     /gene="mglC"
                     /locus_tag="OO575_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6664177.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(27076..28289)
                     /gene="preA"
                     /locus_tag="OO575_001134"
                     /pseudo
     CDS             complement(27076..28289)
                     /gene="preA"
                     /locus_tag="OO575_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(28283..29521)
                     /gene="preT"
                     /locus_tag="OO575_001135"
     CDS             complement(28283..29521)
                     /gene="preT"
                     /locus_tag="OO575_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6664178.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(29715..29954)
                     /locus_tag="OO575_001136"
     CDS             complement(29715..29954)
                     /locus_tag="OO575_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6664179.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(29957..30676)
                     /gene="sanA"
                     /locus_tag="OO575_001137"
     CDS             complement(29957..30676)
                     /gene="sanA"
                     /locus_tag="OO575_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6664180.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(30826..31710)
                     /gene="cdd"
                     /locus_tag="OO575_001138"
     CDS             complement(30826..31710)
                     /gene="cdd"
                     /locus_tag="OO575_001138"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6664181.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(31840..32535)
                     /locus_tag="OO575_001139"
     CDS             complement(31840..32535)
                     /locus_tag="OO575_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6664182.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(32532..32930)
                     /locus_tag="OO575_001140"
     CDS             complement(32532..32930)
                     /locus_tag="OO575_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6664183.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            33169..34116
                     /gene="dusC"
                     /locus_tag="OO575_001141"
     CDS             33169..34116
                     /gene="dusC"
                     /locus_tag="OO575_001141"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6664184.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(34487..34570)
                     /gene="yohP"
                     /locus_tag="OO575_001142"
     CDS             complement(34487..34570)
                     /gene="yohP"
                     /locus_tag="OO575_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6664185.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            34794..36230
                     /gene="mdtQ"
                     /locus_tag="OO575_001143"
                     /pseudo
     CDS             34794..36230
                     /gene="mdtQ"
                     /locus_tag="OO575_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            36283..37044
                     /locus_tag="OO575_001144"
                     /pseudo
     CDS             36283..37044
                     /locus_tag="OO575_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(37161..37739)
                     /locus_tag="OO575_001145"
     CDS             complement(37161..37739)
                     /locus_tag="OO575_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6664186.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            37909..38496
                     /locus_tag="OO575_001146"
     CDS             37909..38496
                     /locus_tag="OO575_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6664187.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            38670..39602
                     /gene="pbpG"
                     /locus_tag="OO575_001147"
     CDS             38670..39602
                     /gene="pbpG"
                     /locus_tag="OO575_001147"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6664188.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(39640..41355)
                     /gene="dld"
                     /locus_tag="OO575_001148"
     CDS             complement(39640..41355)
                     /gene="dld"
                     /locus_tag="OO575_001148"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6664189.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            41551..43848
                     /gene="bglX"
                     /locus_tag="OO575_001149"
     CDS             41551..43848
                     /gene="bglX"
                     /locus_tag="OO575_001149"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6664190.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            44100..45017
                     /gene="osmF"
                     /locus_tag="OO575_001150"
     CDS             44100..45017
                     /gene="osmF"
                     /locus_tag="OO575_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6664191.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            45024..46181
                     /gene="yehY"
                     /locus_tag="OO575_001151"
     CDS             45024..46181
                     /gene="yehY"
                     /locus_tag="OO575_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6664192.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            46174..47100
                     /gene="yehX"
                     /locus_tag="OO575_001152"
     CDS             46174..47100
                     /gene="yehX"
                     /locus_tag="OO575_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6664193.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            47105..>47522
                     /gene="yehW"
                     /locus_tag="OO575_001153"
     CDS             47105..>47522
                     /gene="yehW"
                     /locus_tag="OO575_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6664194.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGA"
BASE COUNT        11417 a        11674 c        12767 g        11664 t
ORIGIN      
        1 gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg cgggggagtt atgcatgctg
       61 gtcgatcact ggaagaggaa gacgcggagt ttacgctgct tgtcagaaaa taaacagaca
      121 gcgaaatcaa aagcgccacg aataatcaaa atgccatttc actgttgctg attggctgct
      181 gacgctgctg aaatgaaatt gctgataacg gattttccca tcagcaatta ggaaaaatga
      241 caaaaaattg tgcagcacat caaacttttg ctcataactt tacggctttc cttgcgtgct
      301 gaaacagaag tattatgctt tcttgaaacg tttcagcgcg atcttgtctt taaccctaag
      361 ctaggttggc gctttttttc tcatagaggc tgaatcgttt caattcagca agagaggaga
      421 actatgttcc agttatccgt acaggacatc catccgggcg aaaaggccgg agacaaagaa
      481 gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg gtaatgtagc agaaggctac
      541 gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt tcctcggcaa tggtattgct
      601 attccacacg gcactaccga cacccgcgat caggtgctga aaaccggcgt tcaggtattt
      661 cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag cgtacgtggc aatcggtatt
      721 gctgccagct cggataaaca tctgggcctg ctacgccagc tgacccacgt actgagcgat
      781 gattccgttg ctgaacaact gaagtcagca acaacagcag aagaacttcg cgcattactg
      841 atgggcgaaa agcagagtga gcagctgaag ctcgacaacg aaatgctgac gctggatatc
      901 gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg cgcgtctgaa agaggcgagg
      961 gcagttgacg ccactttcgt caccaaagcc atcaatgaac aaccgctgaa tctcggacag
     1021 ggtatctggc tgagcgatag cgccgaaggc aatctgcgta gtgcgattgc ggtaagccgt
     1081 gcggcaaatg cttttgatgt ggacggcgaa acggcagcca tgctggtgag tgtggcgatg
     1141 aatgacgatc agcccatcgc ggttcttaag cgtctcgctg atttgttgct cgacaataaa
     1201 gctgaccgct tgctgaaagc ggatgcggca acgttgctgg cgctgctgac cagcgatgat
     1261 gcgccgaccg acgacgtgtt aagcgcggag tttgtggtgc gcaatgaaca cggcctgcat
     1321 gctcgtccag gtaccatgct ggtcaatacc attaaacaat ttaacagtga tattaccgtg
     1381 acaaaccttg atggcaccgg caaaccggca aacggacgta gtctgatgaa agttgtggca
     1441 cttggcgtta agaaaggtca tcgcctacgc tttaccgccc agggtgcaga tgctgaacag
     1501 gcgctgaaag ccattggcga cgctatcgct gctggtcttg gggagggcgc ataatgagca
     1561 gacgtgttgc tacgatcacc cttaatccgg cttatgacct tgttggtttc tgcccggaaa
     1621 ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct gcatgcggcg ggtaaaggca
     1681 tcaacgtggc caaagtatta aaagacctgg gaattgatgt caccgttggc ggcttcctgg
     1741 gtaaagacaa tcaggatggt tttcagcaac tgttcagcga gctgggcatt gccaaccgtt
     1801 tccaggttgt acaggggcgc acccgaatta acgttaagct gacggaaaaa gacggcgaag
     1861 tgaccgactt caacttctcg ggtttcgaag tcacccccgc cgactgggaa cgctttgtga
     1921 ctgattctct gagctggctc ggtcagttcg atatggtctg tgtcagcgga agcttaccgt
     1981 caggcgtcag cccggaagcg ttcaccgact ggatgactcg cctgcgtagt cagtgtcctt
     2041 gcattatctt tgatagtagc cgtgaagcgt tagtagcagg tttgaaagcg gcaccgtggc
     2101 tggtgaaacc taaccgccgc gagttggaaa tctgggcagg ccgtaaactg cctgaaatga
     2161 aagatgtgat tgaagctgca catgcgctac gtgaacaagg tattgcgcat gttgttattt
     2221 cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg cgaatggatc gctaaaccac
     2281 cgtcagtcga tgtcgtaagc accgttggcg caggggattc tatggttggt ggcctgattt
     2341 atggcttgct gatgcgtgaa tccagtgaac acacactgcg tctggcgaca gctgttgcag
     2401 ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg tccgcagttg gccgcaatga
     2461 tggcgcgcgt cgacttacaa ccttttaact gacagcagga gaggcataat gaaaacgctg
     2521 ctgattattg acgctaatct cggtcaggca cgcgcctata tggcgaagac cctgctgggc
     2581 gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc cgaacgacgc agaaatggcg
     2641 attgttctcg gtgattccat cccgaatgac agcgcgctga acggtaaaaa tgtctggctg
     2701 ggtgatattt cccgggcagt tgcgcaccct gagctgttcc tgagtgaagc caaaggccat
     2761 gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg ttgccgccag cggtccgaaa
     2821 cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac acacctttat ggcggctgaa
     2881 gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga aagttgaaac ccgtggttct
     2941 gttggcgcgg gtaatgcaat cactcccgag gaagtagccg cagcggatct ggtgattgtg
     3001 gcggcagata tcgaagtgga tctggcgaaa tttgctggta aaccgatgta tcgtacctct
     3061 accggtctgg cgctgaagaa aacctcgcag gaactggata aagcggttgc tgaagcaacg
     3121 ccgtatgaac cggcgggcaa agctcaaacg gcgaccactg aaggtaagaa agagagtgca
     3181 ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc tgccgatggt cgttgctggt
     3241 ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag cgtttaaaga gccgggtacg
     3301 ttggcagcgg cgctgatgca gattggtggt ggttcagcct ttgcgctgat ggtgccggta
     3361 ctggcaggtt atattgcctt ctccattgcc gatcgtccgg gcctcacgcc gggtctgatt
     3421 ggcggtatgc tggcggtcag caccggttct ggcttcattg gcggtattat tgcgggcttc
     3481 ctggctggtt acattgcgaa gttaatcagt acgcaactga aactgccgca gagtatggag
     3541 gcgctgaaac cgatcctgat cattccgcta atttccagtc tggtggtcgg tctggcgatg
     3601 atctacctga tcggtaaacc agttgctggc attctcgaag ggctgactca ctggctgcag
     3661 accatgggga ctgcgaatgc ggttctgctg ggggcgatcc tcggtggcat gatgtgtact
     3721 gacatgggcg gtccggtaaa caaagcagcg tacgcattcg gtgtgggtct gctgagtact
     3781 caaacctatg gcccgatggc ggcgattatg gcggcaggta tggtgccacc gctggcaatg
     3841 ggcctggcaa caatggtggc gcgtcgcaaa ttcgacaaag cgcagcagga aggtggcaaa
     3901 gccgctctgg tattgggact gtgcttcatt tcggaaggtg caattccgtt tgctgctcgt
     3961 gatccgatgc gtgtgctgcc gtgctgtatc gtgggtgggg cgctgactgg cgcaatctca
     4021 atggcgattg gtgcgaaact gatggcaccg cacggtggtc tgtttgttct gctgatccct
     4081 ggcgctatta cgccggtatt gggttacctg gtagcaatta ttgccggtac gctggtggcg
     4141 ggtttggcct atgccttcct gaaacgtccg gaagtggacg cagtagcgaa agcagcgtaa
     4201 taaaaggtgt gttacagggc agaaatttcc tgccctgtaa catttgatga gcgcaaagag
     4261 aatgaccagc ttgtgaatgt aattttatga atttaaatgg ttttatcttt cagtagaaac
     4321 ttcaaacgtt catcaaacac taaggttatt tcctcaaggg cagattattt tctgcccttt
     4381 tttagtgttc ctgttctgtg catcccgtca caaattcctc gcttttgtaa ttcaggcatt
     4441 taatcgcatt ttgtgagcct gctcaaatct gcccattttg cctctctggc ggtaatttac
     4501 tttacgtaaa acatgcatac tatgagcaca tgtttaaata tgaacacatg tttaaaccgt
     4561 ctttgcagta tctatattct tgtggattga cagggttggt aattttttac cagaggacgt
     4621 gctatgcgcg aaaaggatta tgtcgtaatt ataggttcgg cgaatattga tgtcgccgga
     4681 tattcacatg aatcattaaa ttatgcggat tcaaatccag gtaaaataaa atttacgcct
     4741 ggtggagtag ggcgcaatat tgcacaaaac ctggcgttgc tgggtaacaa agcctggcta
     4801 ctgagcgccg taggcagtga tttttatggt caatcgctgc taacgcaaac caatcaatct
     4861 ggtgtttatg tcgataaatg cctgattgtg ccgggagaaa atacgtcgag ttatttatca
     4921 ttactcgata ataccggtga aatgctggtt gctataaatg acatgaatat tagcaacgct
     4981 attacagctg aatatctcgc acagcaccgt gaatttattc agagggcaaa ggtcattgtc
     5041 gcggactgta atatcagtga agaggcactg gcatggattc tggataatgc cgccaacgta
     5101 cccgtatttg tcgatccggt ttcagcgtgg aaatgtgtca aagtgcgcga ccgtctaaat
     5161 cagaaccaca ctctgaagcc aaaccgcctt gaagcggaaa ccctgagtgg gattgcgctg
     5221 tcagggcgtg acgatgtggc aaaagttgct gcctggttcc atcaacatgg cctgaaccga
     5281 ctggtattga gcatgggcgg cgacggcgtt tattacagcg atatcagcgg tgaaaatggc
     5341 tggtctgcgc cgatcaaaac caatgttatt aatgttaccg gagcgggcga tgccatgatg
     5401 gcgggacttg cttcgtgttg ggtagacgga atgccgtttg ccgaatctgt tcgtttcgca
     5461 cagggatgtt cgtcaatggc gctctcctgt gaatacacca ataaccccga tttatcgatt
     5521 gccaacgtta tatcgttagt ggagaacgca gaatgtctga attaaaaatt tcccctgaat
     5581 tattacaaat ttctccggaa gtgcaggacg ctttaaaaaa caaaaaaccg gttgtggcgc
     5641 tggaatcgac cattatttct cacgggatgc cgttcccaca aaatgcccag accgcaattg
     5701 aagtagaaga aactattcgt aaacagggcg cagtacctgc cactatcgcc attattggcg
     5761 gcgtgatgaa agtgggttta agcaaagaaa aaattgaatt actgggtcgt gaagggcata
     5821 acgtgaccaa agttagtcgt cgcgatttac cttttgttgt tgccgccgga aaaaatggcg
     5881 caaccactgt ggcttcaacg atgattattg cggcgcttgc cggaattaaa gtatttgcca
     5941 ccgggggaat tggtggtgtg catcgcgggg cggaacatac cttcgatatt tctgccgatt
     6001 tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc cggggcgaaa tctattctcg
     6061 atttaggatt aaccactgag tatttagaaa ccttcggtgt gccgttaatt ggctatcaga
     6121 ctaaagcgct gcctgcgttt ttctgccgta ccagctcgtt tgacgtcagc attcgtctcg
     6181 acagcgccag tgaaattgcc cgtgcaatgg cggtgaaatg gcaaagcggg ctgaacggtg
     6241 gcctcgtggt agcgaacccg atcccggaac agtttgcgat gccggaggaa tctatcaatg
     6301 cagccataga tcaagccgtc gccgaagccg aagagcaggg cgttattggt aaagaaagta
     6361 caccgttcct gctggcgcgc gttgctgagc tgaccagcgg tgacagcctg aaatccaata
     6421 tccagctggt gttcaacaac gccattctgg cgagtgaaat tgccaaagaa taccagcgtc
     6481 tcgcgggtta atttctgacg ggcagaaagc cgtcgcccgt actcatcaag cataccaaaa
     6541 cctggcgctg acgccgggtt tactggcatg aagggaaaat cattatggat ataatgagaa
     6601 gtgttgtggg gatggtggtg ttactggcaa tagcatttct gttgtcagtg aataaaaaga
     6661 gcatcagttt gcgcacggtt ggagccgcac tgctgctgca aatcgctatt ggtggcatca
     6721 tgctctactt cccaccggga aaatgggcag tagaacaggc ggcattaggc gttcataaag
     6781 tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg ttcgctggtt gggccgaaaa
     6841 tggatgtcct gtttgacggt gcgggtttta tcttcgcctt tcgcgtactt ccggcgatta
     6901 ttttcgttac tgcgctcatc agtctgctgt actacattgg cgtgatgggg ctgctgattc
     6961 gcatccttgg cagcattttc cagaaagccc tcaacatcag caaaatcgaa tcttttgttg
     7021 cggttaccac tattttcctc gggcaaaatg agatcccggc gatcgttaaa ccgtttatcg
     7081 atcgcgtgaa tcgcaacgag ttgtttaccg caatttgtag cgggatggcg tccattgctg
     7141 gttcggtgat gattggttat gccggaatgg gcgtaccaat tgactacctg ttagcggcat
     7201 cgctgatggc gatccccggc ggtattttgt ttgcacgtat tcttagcccg gcaaccgagc
     7261 cttcgcaggt cacatttgaa aatctgtcgt tcagcgaaac gccgccaaaa agctttatcg
     7321 aagcggcggc gagcggtgcg atgaccgggc tgaaaatcgc cgctggtgta gcgacggtgg
     7381 taatggcgtt tgtcgcaatt attgcgctga tcaacggcat tatcggcgga attggcggct
     7441 ggtttggttt cgccaatgcc tctctggaaa gtatttttgg ctatgtgctg gcaccgctgg
     7501 cgtggatcat gggtgtggac tggagtgatg ccaaccttgc tggtagcctg attgggcaga
     7561 aactggcgat taacgaattc gtcgcttacc tgagtttctc cccatacctg caaacgggcg
     7621 gcacgctgga agtgaaaacc gtggcgatta tatcctttgc gctttgtggt tttgctaact
     7681 ttggttctat cggtgttgtc gttggcgcat tttcggctat ttcgccaaaa cgtgctccag
     7741 aaatcgccca gcttggttta cgggcgctgg cagcagcaac gctttccaac ctgatgagtg
     7801 cgactattgc cgggttcttt attggtctgg cgtaatgctc gatttgtaga ccggataaaa
     7861 cgtattaagc gttgcatccg gcaatcgcgc tattgcaaat aatttttact gcatcatccc
     7921 ggagaattta ttctccgggt ccatctccag cgccagtcct gacaactgct ttctgttttt
     7981 aattagatat cctttctttt aattaataaa ccgtcgtgaa tgaattgcgc gagaacatat
     8041 aaaagatgtc gataagaaac gcctaaatac tctgccgctt gcgtatgctt ttcgtgataa
     8101 agatcacctt cttgtgagag taatataaat gctgccaggc gattcactaa cggaaatgat
     8161 tgattctgag ttaaagaaac aatattacga taatttttat gacttaaggt gacgcaaagt
     8221 tttcgtaaaa atagggtgtc gtttaataac agcggacggt aatgtttcat agggagcgca
     8281 aggcaccaac actcttcaat agcctgcacc gcacgcggtt catggtcttt gtcgattaac
     8341 tcaatctcgc caataaaaca gggagcggca aagaaatcga tcagcgacac gcgaccattg
     8401 gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat aaaacagcca tgacggctgt
     8461 tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa acagccgcgt atcggctgaa
     8521 acatccgttg aaaagcaatc cttaaacgca gattcactca tcaattgctg cttaaggtca
     8581 ttattgtgga tttctttcac gatgtttatc cgctatgaga tttctcatat ttagcatacg
     8641 tacgctgttg ttaagattca atcattggcc cggtcgaatt ttctttgctg tagaaaaaaa
     8701 taacgcaact ggaaacagag gaaataaaca atggaaaaga gaaaaattat tctggattgt
     8761 gagccgggtc atgatgatgc tattgcaatg atgatggcag cgaaacatcc ggcaatagat
     8821 ttattaggca tcactattgt agcgggtaat cagacgcttg ataaaacatt aattaatggc
     8881 ctgaatgttt gccagaaact ggagattaat gttccggttt atgcggggat gccgcaaccc
     8941 attatgcgta aacaaatcgt tgccgataat attcacggtg aaaccggact ggatggcccg
     9001 gtattcgagc cgctgacccg ccaggcagaa agtacccatg cggtgaaata tatcatcgat
     9061 accctgatgg caagcgatgg cgatatcact ctggtgccgg ttggtccgct ttcaaatatc
     9121 gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa tccgtgaaat tgtgctaatg
     9181 ggcggcgctt acggtacagg caacttcacc ccatctgccg agttcaatat ctttgccgac
     9241 ccggaagccg cccgcgtggt gttcacctcc ggcgttccat tagtgatgat gggcctcgat
     9301 cttaccaacc agaccgtttg caccccggat gtaattgctc ggatggaaag ggtaggcggc
     9361 cctgccggag agctgttcag cgacatcatg aacttcactc tcaaaacgca gttcgaatac
     9421 tacggcctgg ctggcggccc ggtgcatgac gccacctgca ttggttatct gatcaaccct
     9481 gatggcatta aaacccagga tatgtacgtc gaagtggacg tcaacagcgg cccttgctac
     9541 gggcgtacgg tctgcgacga actgggcgtt cttggcaagc ccgctaatac caaagtcggc
     9601 atcactattg ataccgactg gttctggggc ttagtcgaag agtgcgtgcg cggctacatc
     9661 aaaacccatt aagtgttgat aacaagccgg gacagtatcg caagatgcag gcccggctat
     9721 ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag agataatcac tatggatgtc
     9781 atgagaagtg ttctgggaat ggtggtactg ctggcaattg cgtttttact gtcagtaaac
     9841 aagaagaaga tcagcctgcg taccgttggc gcggcgttag tgttacaggt cgtgattggc
     9901 gggattatgc tttggttacc gccagggcgt tgggtcgcag aaaaagtcgc ttttggcgtc
     9961 cacaaagtga tggcgtacag cgacgcaggt agcgcattta tcttcggttc tctggtcgga
    10021 ccgaaaatgg ataccttatt tgatggcgca ggatttatct ttggtttcag ggtattaccg
    10081 gcaattatct tcgtcactgc actggtgagt attctctact acatcggtgt gatggggatc
    10141 ttaattcgca ttctcggcgg tatcttccag aaagctttaa atatcagcaa gatcgagtca
    10201 ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa ttccggcgat cgtcaaaccc
    10261 tttatcgatc gtctgaatcg caatgaatta tttacagcga tttgtagtgg catggcctcg
    10321 attgctggtt cgacaatgat tggttacgcc gcactgggcg tacctgtgga atatttgctg
    10381 gcggcatcgt taatggcgat ccctggcggg atcttgtttg cccgcctgtt aagcccggct
    10441 acggaatctt cgcaggtttc ttttaataac ctctctttca ccgaaacacc gccaaaaagc
    10501 attattgaag ccgatgcgac aggggcaatg accgggctga aaatcgccgc cggtgtagcg
    10561 acagttgtta tggcatttgt cgccatcatt gcgttaatta acggtattat cggcggcgtt
    10621 ggcggctggt ttggttttga acatgcctcg ctggagtcca ttttaggtta cctgctggcc
    10681 ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa atcttgccgg gagtttgatt
    10741 ggacagaaac tggcaataaa tgaatttgtc gcttatctca atttctcacc ctatctgcaa
    10801 acggctggca ctctggatgc taaaaccgtg gcgattattt ccttcgcgtt gtgcggtttc
    10861 gctaactttg gttctatcgg ggtggtggtg ggggcgtttt ctgcggttgc gccacaccgt
    10921 gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg cggcgacact ttctaatctg
    10981 atgagtgcca ccattgccgg gttctttatt ggtttagcgt agtgtgcaat ttatgagccg
    11041 gattagatgc ggtcaagcgt cgaatccggc aacattgtta caccattggc actaatgaca
    11101 gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc gctggcgcgt gaaattgccg
    11161 cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt tccctccaga aagctatata
    11221 ccagacccgc cataaaacca tcgtcagcac caaaactgtc taccgtcgta tgcgctggca
    11281 cagtcagcaa aaattgttca ccatcctttt cgctgcaata aactgactca tcgggcaaat
    11341 aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt cactgcggca ttacggtcag
    11401 catcgctggt gatcgcctgt ccccataaaa tttccagctc cggtaaagtg ggtttcaggg
    11461 tgtgaatatg cgccagccag tgtttgattt tgcccgcttt gaattctgaa acggtatcga
    11521 caaacaccgg gatctcatca gcgagggtaa agacccattc cagcgcctcg gctgtcaggt
    11581 tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc gcgcgacccg ttcagtagtt
    11641 gcggtgtcaa ctgttccagc agatgggtat cgttaattgc cagcacggtt tgatcgtctc
    11701 gattggcaat tgccagatac gtcgatgtgc tttgaccatg caaacgaacg cagccggaga
    11761 cattcacgcc ggcacggcgc gtttcttcca ggagcatttc gccataaaag tcatcgccaa
    11821 tcactgaaag caaatggacg tcacggccta acagcgccag attgtgggcg atattgcgtc
    11881 caacgccgcc tgccgaacaa tgaattgtac cgggatgaga agccgcttgc gggtaatgga
    11941 tatccgccat cccgcgaata tccatattga ttgtccccac cactacgcag tattcctgct
    12001 cggtgagaat gtaacctttg tctttaatcc ggcctttgcg cattaaatcc ataatatgcg
    12061 ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat ttcgttctgc tgaatcagcg
    12121 ggttacgccg taaaattgca aggatctcct tttcccggtt atttatcttc aggctaccgc
    12181 tttttcagtt tgttgcgctt tcagccaggc gatctcttct gcccagatat ccgggttgat
    12241 ggtttcaagg atcagcggaa tgccgtcgaa acggtcgttc tgcataatcc agcggaacgc
    12301 atcatgaccg atattgcctt caccgaggct atgatggcgg tcaacgcggc tgccaaaggt
    12361 acttttcgca tcgttaaggt gcatcccgcg caaatactta aagccgacaa tacgggcaaa
    12421 atcagcgaac gttttttcgc attcggctgg agtacgcaaa tcatacccgg cagcgaaagc
    12481 atggcaggta tcaatgcaga cgccgacgcg ggatttatct tccacgccgt cgataatcgc
    12541 cgcgagatgt tcgaatttaa accctaagtt actgccctga ccggcggtgt tttcaatcac
    12601 cgctgttaca ccttgagttt tatccagcgc aatgttgatg gattcggcaa tacgcgcaag
    12661 gcaatcctct tctgaaatct gcatcagatg gctgccaggg tggaagttga gcaaagaaag
    12721 ccccagttgt tcgcaacgct gcatttcatc tataaaggca tcgcgcgatt tttccagagc
    12781 ttcagtgacc ggatgtccga ggttgatcag ataactgtcg tggggaagaa tttgcgccga
    12841 tgtgtagtga tatttttcac aggcggcttt gaattcatcg atggtttgcg tcgtgagcgg
    12901 tgcggcacgc cactgacgtt ggtttttggt gaacaaggca aacgcggttg cgtcgatttc
    12961 ggcggcgcga attgcggcat ttgccagacc gccagcagca ctaacgtgcg ctccaatgta
    13021 tttcatgcga ggactcctgt taaacccgct ggaggaaaac ggtaatgata gcgggttaac
    13081 aggagcaaga agtagtggtt tatgcgatga cgctttgaat cacatagtta atcgcaccac
    13141 caccaacaat cagccaggca aacagtacca gtgccatcag cagaggtttc gccccagctt
    13201 ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc catcgccatt gccagcagga
    13261 aggtatccag cgttaccagc atgttcacca cgctctgcgg taacaggtgg aacgagttaa
    13321 agatggcaac tacgatgaac aagatggcaa accacggaat agtgattttg cttttctcgc
    13381 cgctgttcgc cccagacagc tgtttaacac gcgccgccag caggatgagg aacggagcca
    13441 gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt ttctgcatcc gggctgatgg
    13501 catgacctgc cgccaccacc tgcgccactt cgtgcacagt agaaccaatg tagataccga
    13561 aagtttccgg actaaaccat tgagacatca gcggatatat cgccgggtag aggaaaatcg
    13621 cgacggtccc gaagataaca acggttgcaa cagccacggt tactttactg gcttccgctt
    13681 tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca gatactgcta ccggcaccga
    13741 tcaaccaact ggtgtgctta tccagaccaa acactttctg ccccaggaag caagccagca
    13801 ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc actgataccg acatcggcaa
    13861 tttgcgagaa cgtcagacgg aagccataaa gaatgatacc cagacgtaat aaatattgct
    13921 tggcaaacag cacaccaccg tcacagcttt tccagatgtg cggatagatg gtgttgccta
    13981 aaaccatccc caacaagatt gcgagggtga gggcactaaa cccggcaccc gcaaccgcgg
    14041 gaatggaacc accccacagg gcgaccccgg tgataactgc actcagggct aaccccggaa
    14101 taaaatgcca cagtgtacga tgttgtttct gtaaggtgat attcgtcata accctctcct
    14161 ttaaccgggc ataaggttac gactaactgg tttaaaaata aaattgatta tatatttata
    14221 attaatcttt ataagtggta agcgactatg cacatcaccc tccggcagtt ggaagttttt
    14281 gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg tgatgctggc gttgtcgcaa
    14341 tcagcagtga gcgcagcctt gactgacctg gaagggcagc ttggcgttca actgtttgat
    14401 cgcgtgggga aaagactggt tgttaatgaa cacgggcggc tgctctatcc gcgagcgttg
    14461 gcactgcttg aacaggcggt tgaaatcgaa caactgtttc gcgaagacaa cggcgcgatt
    14521 cgtatctatg ccagtagtac catcggtaac tacattctgc ctgcagttat cgcccgttat
    14581 cgccatgatt atccgcagtt gccgattgaa cttagcgttg ggaacagcca ggacgtgatg
    14641 caagcggtgc tggatttccg cgttgatatt ggctttattg aaggaccgtg ccacagcact
    14701 gaaatcattt ctgaaccgtg gctggaagac gagctggtgg ttttcgccgc gccgacttcg
    14761 ccgttggctc gtggtccggt caccttagaa cagctggccg ctgcgccgtg gatcctgcgt
    14821 gaacgcggtt ccggcacgcg ggatattgtc gattatctgt tgctgtcaca tttaccgaag
    14881 tttgagatgg cgatggaatt aggtaactcc gaggcaatca aacatgcggt gcgtcatggg
    14941 ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc aattgcaggc aggcacatta
    15001 agtgaagttg cggtccctct gccgcgcctg atgcgtacgt tgtggcgtat acatcatcgg
    15061 caaaaacacc tttccaacgc gctacggcgc tttctggact attgcgatcc cgcaaatgtg
    15121 ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt cgatttcgtg acgcagcgcc
    15181 ttcagcatgc attcgccaga aaagagattg gctgctttac ttataatccc tgggcgatca
    15241 tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt gccaatcgtc tgctacaatc
    15301 gcgccatcat ttttaagatg gatagcattt ttgtatgggt tccgaaacta aaactacaga
    15361 agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg acgatgattg ccattggcgg
    15421 ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg atttctcagg caggtccggg
    15481 cggggcattg ctctcgtata tgctgattgg cctgatggtt tacttcctga tgaccagtct
    15541 cggtgaattg gctgcatata tgccggtttc cggttcgttt gccacttacg gtcagaacta
    15601 tgttgaagaa ggctttggct tcgcgctggg ctggaactac tggtacaact gggcggtgac
    15661 tattgccgtt gacctggttg cagcacagct ggtcatgagc tggtggttcc cggatacacc
    15721 gggctggatc tggagtgcgt tgttcctcgg cgttatcttc ctgttgaact acatctctgt
    15781 tcgcggcttt ggtgaagcgg aatactggtt ctcactgatc aaagtcacga cagttattgt
    15841 ctttatcatc gttggtgtgc tgatgattat cggtatcttc aaaggcgcgc agcctgcggg
    15901 ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt ggttttgcgg cgatgatcgg
    15961 cgtagctatg attgtcggct tctctttcca gggaaccgag ctgatcggta ttgctgcagg
    16021 cgagtccgaa gatccggcga aaaacattcc acgcgcggta cgtcaggtat tctggcgaat
    16081 cctgttgttc tatgtgttcg cgatcctgat tatcagcctg attattccgt ataccgatcc
    16141 gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt ccgttcaccc tggtgttcca
    16201 gcacgcgggt ctgctctctg cggcggcggt gatgaacgca gttattctga cggcggtgct
    16261 gtcagcaggt aactccggta tgtatgcgtc tactcgtatg ctgtacaccc tggcgtgtga
    16321 cggtaaagcg ccgcgcattt tcgctaaatt gtcgcgtggt ggcgtgccgc gtaatgcgct
    16381 gtatgcgacg acggtgattg ccggtttgtg cttcctgacc tccatgtttg gcaaccagac
    16441 ggtatacctg tggctgctga acacctccgg gatgacgggt tttatcgcct ggctggggat
    16501 tgccattagc cactatcgct tccgtcgcgg ttacgtattg cagggacacg acattaacga
    16561 tctgccgtac cgttcaggtt tcttcccact ggggccgatc ttcgcattca ttctgtgtct
    16621 gattatcact ttgggccaga actacgaagc gttcctgaaa gataccattg actggggcgg
    16681 cgtagcggca acgtatattg gtatcccgct gttcctgatt atttggttcg gctacaagct
    16741 gattaaagga actcacttcg tacgctacag cgaaatgaag ttcccgcaga acgataagaa
    16801 ataagtttcc tcccttcctt gctaagccct ctcaaccgag agggcttttt caattccatt
    16861 tccctgacaa atcatgcgga tataaaattt aacatttgga ttgataattg ttatcgtttg
    16921 cattatcgtt acgacgcaat caaaaaaggc tgacaaatca gaggctgttc cggctttctg
    16981 ggatgatcac ctgcatacaa ataagtccac cgcgatgctg ccgtacgcaa ggggacgtaa
    17041 agaagatgtg agcgataccc attttatttt cgtagttacc tcatggagat atggaatgtt
    17101 taggttgaac cctttcgtac gggtcgggct gtgtttgtcc gctatttctt gtgcatggcc
    17161 tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc actgcatctt ccgtggaaca
    17221 aaatcttaaa gatgcacccg ccagtatcag cgtcattacc caggaagacc tgcagcgaaa
    17281 accggtacag aatctgaagg atgtcctcaa agaagtgcct ggcgtacaac tgacgaacga
    17341 aggggataac cgtaagggcg ttagtattcg tggtctggac agcagctaca ccctgattct
    17401 tgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc cgccacaatg atttcgatct
    17461 gaactggatc ccggtcgatt ccatcgaacg tattgaagtg gttcgtggcc cgatgtcgtc
    17521 gctatacggc tccgatgcgc tcggcggtgt agtgaatatc atcaccaaaa aaatcggtca
    17581 gaaatggtcg ggtaccgtta ccgtcgatac caccattcag gaacatcgcg atcgcggtga
    17641 tacctataac ggtcagttct ttaccagtgg accattaatt gatggtgtgc tgggaatgaa
    17701 agcttacggc agcctggcaa aacgtgaaaa ggatgacccg caaaactcaa cgaccaccga
    17761 taccggagaa acgccgcgta ttgaaggatt ctccagccgc gacggcaatg tcgaatttgc
    17821 ctagacaccg aatcaaaatc acgattttac tgccggatac ggtttcgacc gtcaggatcg
    17881 tgattccgat tcgctggaca aaaaccgcct ggaacgccag aactactgcg tcagccataa
    17941 tgggcgttgg gattacggca ccagcgaact gaaatcctac ggtgagaaag tcgagaacaa
    18001 aaaccctggc aacagcagcc cgataacttc cgaaagcaat acggtcgacg gcaaatacac
    18061 gttgccgctg acggcgatta atcagtttct cacggttggc ggtgaatggc gtcacgacaa
    18121 acttagcgat gcggtgaacc tgaccggggg aaccagctcc aaaacgtctg ccagccagta
    18181 cgcgctgttt gtggaagatg aatggcggat cttcgagccg ctggcgctga cgaccggcgt
    18241 gcgtatggac gatcacgaaa cctacggtga acactggagt ccgcgtgcct acatggttta
    18301 taacgccacc gacaccgtaa cggtgaaagg gggctgggcg acggcattta aagcaccttc
    18361 tctgttgcaa cttagccctg actggacgag caattcctgc cgtggcgcat gtaagattgt
    18421 gggtagcccg gatctgaaac cagaaaccag cgaaagttgg gagctggggc tttactacat
    18481 gggtgaagaa ggctggctgg aagggggtga atccagcgtt atcgttttcc gtaacgatgt
    18541 gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac gctgcaccgg gctaccaaaa
    18601 ctttgttggt tttgagacgg gcgctaacgg acggcgcata ccggtattta gctactacag
    18661 cgttaacaaa gctcgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga
    18721 atggaaactg tcgatcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa
    18781 caaaccgcta tccgatctgc cgttccatac tgctaacggt acgctggact ggaaaccgct
    18841 ggcgctggaa gactggtcat tctatgtttc tggtcactat accgggcaga aacgcgccga
    18901 cagcgcgacg gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca
    18961 ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag
    19021 tcgtgacgac tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg
    19081 cttctgatga gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgcgca
    19141 tggaagcgca ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc
    19201 ggctgaatac gcagcgtcat cggccacgct ttctggcgcg cggcttctgc caataccgca
    19261 ggttgcaact ggtcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc
    19321 gcatcctgcg cgttactggc atacatcagc gcgcaactat cccattccag catgcatttt
    19381 tgtcctcacc taaataggtg ctaaacgctt tgattcccca cgggacgctg cacggattca
    19441 caatgggcgc aaaggccgaa acgctggtgt atttacccgg atttttcagc gccataatca
    19501 gcgcaccgtg accacccatt gagtgaccgc taatggcgca gcggtcgctg acattaaatt
    19561 gcgactgaac cagcgccggt aattcatcgc gcagataatc atacatccgg taatgcgtcg
    19621 cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc ctggcctaaa tcgtagccat
    19681 cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg catcaccagt acaatcccca
    19741 gttccgccgc tacccgctgg gcacccgcct tggtggtgaa gttctcgtca ttgcaggtta
    19801 atccggaaag ccagtacagc actggtggcg gggtgtgatc acgagggggg gggagaaaga
    19861 tactgaacgt catcgggcag tttaaggtac tggagtcgtg tcgccagcgt tgctgccagc
    19921 cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg ctccttgttg tgttgtttgc
    19981 aatttcttca tcataccgtt tattcaatgt gctgtgagta actttcactt ccgtatttgc
    20041 ataacgatgt tttaacatct gctgatgaaa ggcagcggca attacaataa ttatcgctgt
    20101 gaatactgga ttatgtgcgc cgcctcacgc acaataatca agctgtaaat cagcttaata
    20161 actttgcccc cacgcagggt ggaggcgtca cacctgcagg agaaatcata aatgccatca
    20221 ctcagtaaag aagcggccct ggttcatgaa gcgttagttg cgagaggact ggaaacaccg
    20281 ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca aaagccttat tgctggtcat
    20341 atgaccgaaa tcatgcagct gctgaatctc gacctggctg atgacagttt gatggaaacg
    20401 ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct ccggtctgga ttacgccaat
    20461 ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg atgaaatggt caccgtgcgc
    20521 gatatcactc tgaccagtac ctgtgaacac cattttgtta ccatcgatgg caaagcgacg
    20581 gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa aaattaaccg tatcgtgcag
    20641 ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc agcaaattct tattgcgcta
    20701 caaacgctgc tgggcaccaa taacgtggct gtctcgatcg acgcggtgca ttactgcgtg
    20761 aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa cgacctctct tggtggattg
    20821 ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg ctgtgcgcca tcacaactga
    20881 ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat tttgttcgcg gcgtcgccat
    20941 tctggggatc ctgctattaa acatcagcgc ctttgggcta ccaaaggcgg cttatctcaa
    21001 tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg acctgggcat ttctcgatct
    21061 catcggccag gtgaaattcc tcacgctttt tgcgctgctg tttggtgctg gcctgcaaat
    21121 gttgctgccc cgtggcagac gctggatcca gtcgcggtta acgctgttag tcttgttggg
    21181 ctttattcac ggtttattgt tctgggacgg cgatattctg ctggcttacg ggctggtggg
    21241 gcttggcgtt ttgctgttat tggggctgat ttccgacagc cagaccagcc gcgcctggac
    21301 gccggatgca tcggctattt tgtatgaaaa atactggaag cttcacggcg gcgttgaagc
    21361 gatcagtaat cgtgccgatg gtgttggcaa cagtttactg gcactgggcg cacagtatgg
    21421 ctggcaactg gcagggatga tgctcattgg tgcggcgctg atgcgcagcg gctggctgaa
    21481 agggcagttc agcttacgtc actatcgtcg tactggtttt gtgctggtgg cgattggggt
    21541 gaccattaac cttcctgcca tcgccctgca atggcagctg gactgggcgt atcgctggtg
    21601 tgccttctta cttcaaatgc cgcgggaact gagtgcgccg tttcaggcga ttggctatgc
    21661 gtcgctgttt tatggcttct ggccgcaatt gagccgcttt aagctggtgc ttgcgatcgc
    21721 ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa acgctgattt gtaccacgct
    21781 tttttaccac ctcggtttgt ttatgcattt tgaccgcctg gagctgctgg cgtttgttat
    21841 tccggtatgg ctggcgaata tcctcttctc tgttatctgg ctgcgttact tccgccaggg
    21901 gccggtggaa tggctctggc gtcagttaac tttgcgtgct gccggaccgg caatatctaa
    21961 aacatcaaga taacgatctg gattacattc gttaacaaaa cggctgtaac cgtttccatt
    22021 gctgtgactc gattcacgaa gtcctgtatt cagtgctgac aaaatagccg ccagcaagca
    22081 gtcatttact gcaatctcat aacaggtagt gaatatgatc accattcgtg atgtagcgcg
    22141 tcaggctggc gtctctgtgg caacggtttc ccgggtgctc aataacagca cgctggtcag
    22201 tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag ctggattatc ggccaaacgc
    22261 caatgcccag gcgctggcaa ctcaggttag cgacaccatt ggcgtggtgg tgatggacgt
    22321 ttctgatgcg tttttcggcg cgctggtaaa agcggtggat ctggtcgccc agcagcatca
    22381 gaaatacgtg ctaatcggca atagctatca tgaagcggaa aaagagcgtc acgccattga
    22441 ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac tcaaaagcat tgagtgacga
    22501 tgaactggcg caatttatgg ataacattcc cggtatggtg ttaatcaacc gcgttgtgcc
    22561 ggggtacgcc catcgttgcg tttgcctgga taatctcagc ggtgcccgaa tggcgacgcg
    22621 catgttgctg aataacggtc atcaacgtat tggttatctt tcttccagcc acggcattga
    22681 agatgacgcc atgcgtaaag caggctggat gagtgcgttg aaagagcagg atattattcc
    22741 gccggaaagc tggattggca ctggtacgcc ggacatgccg ggcggtgagg cggcgatggt
    22801 tgaactgctg gggcgcaatc tacaacttac cgctgtattt gcttataacg acaatatggc
    22861 cgctggcgca ctgacagcat taaaagataa tggcattgcg attccgttac atctctcaat
    22921 catcggtttc gatgatattc ccatcgcccg ttacaccgac ccgcaattaa cgaccgtgcg
    22981 ttatcccatt gcttcaatgg ctaaattagc caccgaactg gccttgcagg gggcagcagg
    23041 caatattgat cctcgtgcca gccactgttt tatgccgacg ttagtgcgtc gccattctgt
    23101 cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca aatcaggcga tgtaaccgct
    23161 ttcaatctgt gagtgatttc acagtatctt aacaatgtga tagctatgat tgcaccgttt
    23221 taatgttgta acccgtatgt aacagtgaat aatcactttc accgaggtaa tagcgtcata
    23281 acaacaatta aagccgtttt ctggagcgtt accgggcatg gaagaacgaa ttttaaaaag
    23341 tgagcttcgg cgttcagtaa cacttcatta actctactgc cccgccgagc atttatctca
    23401 agcactaccc tgcataagaa aaaccggaga taccatgaat aagaaggtgt taaccctgtc
    23461 tgctgtgatg gccagcatgt tattcggtgc cgctgcacac gctgctgata ctcgcattgg
    23521 tgtaacaatc tataagtacg acgataactt tatgtctgta gtgcgcaagg ctattgagca
    23581 agatgcgaaa gccgcgccag atgttcagct gctgatgaat gattctcaga atgaccagtc
    23641 caagcagaac gatcagatcg acgtattgct ggcgaaaggg gtgaaggcac tggcaatcaa
    23701 cctggttgac ccggcagctg cgggtacggt gattgagaaa gcgcgtgggc aaaacgtgcc
    23761 ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg gatagctacg acaaagccta
    23821 ctacgttggc actgactcca aagagtccgg cattattcag ggcgatttga ttgctaaaca
    23881 ctgggcggcg aatcagggtt gggatctgaa caaagacggt cagattcagt tcgtactgct
    23941 gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc acttacgtga ttaaagaatt
    24001 gaacgataaa ggcatcaaaa ctgaacagtt acagttagat accgcaatgt gggacaccgc
    24061 tcaggcgaaa gataagatgg acgcctggct gtctggcccg aacgccaaca aaatcgaagt
    24121 ggttatcgcc aacaacgatg cgatggcaat gggcgcggta gaagcactga aagcacacaa
    24181 caagtccagc attccggtgt ttggcgtcga tgcgctgcca gaagcgctgg cgctggtgaa
    24241 atccggtgca ctggcgggca cagtactgaa cgatgctaac aaccaggcga aagcgacctt
    24301 tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct gatggcacca actggaaaat
    24361 cgacaacaaa gtggtccgcg taccttatgt tggcgtagat aaagacaact tggctgaatt
    24421 cagcaagaaa taagactgat tgggcgcagt cgtttactgc gcccttattg tatggccaac
    24481 aaggtaaaat tatatggtca gctcaacgac tccgtcttcc ggggaatact tgttggaaat
    24541 gagcggtatc aacaagtcct ttcctggtgt taaggcactt gataacgtta atttaaaagt
    24601 ccggccacat tctatccatg cattaatggg ggaaaacggt gcaggaaaat cgacattatt
    24661 aaaatgcctg tttggtattt atcaaaaaga ctccggcacc attttattcc agggtaaaga
    24721 gatcgatttc cattccgcca aagaggcact ggaaaatggt atttcgatgg tacaccagga
    24781 gttaaacctg gtattacaac gttcggtgat ggataacatg tggctggggc gatatcccac
    24841 caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa accaaagcga tttttgatga
    24901 actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca ttatccgttt cgcaaatgca
    24961 gatgatcgaa atcgccaaag cgttttccta taacgcgaaa attgtgatta tggatgaacc
    25021 gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc actattattc gtaaattaaa
    25081 agagcgcggc tgcggtattg tttatatctc gcataaaatg gaagaaatct tccagttatg
    25141 tgatgaagtt accgtattgc gcgacggtca gtggatcgcc accgaaccgc tggcaggact
    25201 gacgatggac aagatcatcg ccatgatggt tgggcgttct cttaatcagc gttttcctga
    25261 caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt aacctaacgt cactgcgcca
    25321 gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg gagatcctcg gtattgccgg
    25381 gctggtgggg gcgaaacgta ccgatattgt tgagacgtta tttggtattc gcgagaaatc
    25441 ggctggcacc attacgttgc acggcaaaca gatcaataac cataatgcca acgaagccat
    25501 aaaccacgga tttgcactgg taactgagga gcgccgctca acgggaattt atgcctatct
    25561 ggatattggt tttaactcgt taatttccaa tattcgcaac tacaaaaata aagttggttt
    25621 actggataac tcgcggatga aaagcgatac ccagtgggtg attgattcga tgcgggtaaa
    25681 aacgccgggt catcggacgc aaattggttc gctctccggt ggtaatcagc aaaaggtgat
    25741 tattggtcgc tggctactaa cgcaaccaga aatattaatg ctcgatgaac cgacgcgcgg
    25801 tattgatgtc ggggcgaagt ttgaaattta tcagttaatt gccgaactgg cgaagaaagg
    25861 caaggggatt attattatct cctctgaaat gcctgagttg ttagggataa cagaccgtat
    25921 tctggtcatg agcaatggtc tcgtttccgg aattgtcgat acaaaaacaa caacgcaaaa
    25981 cgaaattctg cgtcttgcgt ctttgcacct ttaagattag gggcttccca tgagtgcgtt
    26041 aaataagaaa agttttctta cttacctgaa agagggcggt atttacgtcg ttcttttagt
    26101 tttgctggcg attattattt tccaggaccc aacattttta agtctgttga acttaagtaa
    26161 tattctcacc cagtcatcgg tgcgtattat tatcgcgctc ggtgtggcag ggttaattgt
    26221 cacccagggg accgatcttt ctgctggtcg tcaggtaggg ctggcggcag tggtggctgc
    26281 gacattattg cagtccatgg ataacgccaa caaagtgttc ccggaaatgg cgacgatgcc
    26341 gattgcgctg gttattctga ttgtctgtgc cattggtgcg gtgatcggtt tgatcaacgg
    26401 tctgattatc gcttatctca acgtgacgcc gttcattacc acgctcggca cgatgatcat
    26461 cgtctatggc atcaactcgc tctattacga ctttgtcggg gcgtcgccaa tttctggttt
    26521 tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg ctggggagtt tccgtctctc
    26581 ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc tgggtgttgt ggaacaaaac
    26641 ccgcttcggt aagaacattt ttgccattgg cggtaacccg gaagcggcaa aaatatctgg
    26701 tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg tctggcgtgt tctatgcctt
    26761 tggcgggatg ttagaagccg gacgtatcgg ctctgccacc aacaacctcg gctttatgta
    26821 tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta tcgttcagcg gcggtgtggg
    26881 gacggtgatt ggcgtggtga ccggggtaat tatttttacc gtcatcaact atggcctgac
    26941 gtatatcggc gtaaacccat actggcagta catcatcaaa ggggcgatta ttatcttcgc
    27001 cgtagcgctg gattcactga aatacgcgcg taagaagtga tgtgattgcg gatattgccg
    27061 gatgcgtctc tggttttaca acgttaccgg gtgttctttc tcgcctttct taaacttcac
    27121 ttccccgagc tcaatacaac ccaccgggca gacgtgacca caaagcagac aacccacaca
    27181 tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc cattccatcg cctggtgacc
    27241 gccgtcgtaa caggaaatat aacagcgtcc acagccaaca catttgtcaa gattgatacg
    27301 gggatagaca atataactgc ggtctaaatc ttccgccggg acaatattgt tattcgccag
    27361 acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg agataatgcg ataacccgct
    27421 cgccatatct tctacgatgc gatagccgta ctgcataatg ccggtggtga cctgtaacgt
    27481 tgctgcgccg agcaataaaa actccgccgc atcttcccag gtttcaatgc cgccgatacc
    27541 gctgattggg aaatcgcgca gttctggatg ggtgcgcatt tgctgaatga agcgcagggc
    27601 gatcggtttt accgctttac cggaatatcc ggaaatactc gatttaccgt taacgatcgg
    27661 cataccgatt ttctgattaa gatcgatatt ggtgatggat ttaacggtgt taattgccgc
    27721 aatgccatct gcgccgccgc gcttcgccgc cagcgccact tcgcacatat caccgatatt
    27781 cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt ttcactgccc gacaatattt
    27841 ttctaccagc tccgggcttt gcccgacatc gctacccatc gcatgagaag tcatttgcgg
    27901 acaggagaag ttacactcga tcatatccgc gccagctgct cccattgctg ctcattttcc
    27961 cccatgatcg aagcgatcaa tactttgtcc gggtaatctt ccttcagccg acgcagggcg
    28021 gtcagattct cttccaacgg atgttcagca atctgctcca tatttttgaa gccgataaaa
    28081 ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga cttcgttggc gataaaaaag
    28141 ccgatcgttt taaacaccac accgccccaa cctgtgtcgt aggctttggc acacatctca
    28201 tagcagttgc ctaccggcga agaagagagg cagaacgggt tgggaaagtt cacgccgcaa
    28261 aaagtaatcg aaagatcttt cgttaacatg agcaagctcc ctctaaatag tgatgaatcg
    28321 cctcggcggc ttctttcccg gttttcacgg catagaccac ggttttgtca ccctcaacaa
    28381 tatcgccagc agcaaagact tgcggatcgc ggatctgata attttgtgtt ttgatggtgt
    28441 tacgctgctg ctctaactcc gcaaaggcat ccagtctggc atgctgaccg acggcgagaa
    28501 taattttatc tgccgccatc gtcagttcgc ctgataaccg tacatgctta aacgtgactt
    28561 tattgccttc cacggctact ggcgtgaatc catcgatgat cgaaacgccc agctcccggg
    28621 cgctggcaaa ctctttttcg cttgccggaa actcatctaa ctcttcacgt gctacgcaag
    28681 ttaccgcctg acagccgaga actttcagcg tgctggctac gtccatcgcg acatcaccgc
    28741 cgccgataat taatgcgctt tgtggaatgc tgatatcgcc ttgcgcctgt cgtacacgtt
    28801 gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa cagcggtagc ccggaaccgc
    28861 ttgataaccc cacggtgacc agtaccgcgc ggttttccgc tttcagttgc tccagggtga
    28921 gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc cattttttca atacgggcga
    28981 tctctgcatc cagcactgat tgcggtaacc ggaattgcgg aataccgtta cgcaaccagc
    29041 caccggggtg cgcttctttc tcataaatcg tgacgtcata accctggttt gtcagtgtca
    29101 cactggcctg caatccggca ggacctgcgc caataatcgc gactttgccg agcgttttag
    29161 taccgggctg ataaatttcc atcccggttt gttgttcaaa atcagtaaca aaacgctgta
    29221 agcggccgat atcaatggga gcatcaacac cggcacgggt acaaccgctt tggcataatt
    29281 tctccgtcgg gcacactctg gcacaaacgg caccgagggc attattttcg cgaattgtct
    29341 cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat aaatttcccc ggatcggtct
    29401 gtgccgggca agcctgacta cagggagcgt cgtgacataa taaacagcga gaggcttctt
    29461 taatcgccag taacggcgta aaagccggag tgagttcatc cagataattt tgttgcggca
    29521 tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc taaataaata ttgcgtgctg
    29581 atattaaggc tatgcgcgtg gggaaattta agctgtgaaa ggattcacaa atagctgcgg
    29641 ttcagaatgt tatatccgca gcttattgag tgaagtaata attattctca ataattaaca
    29701 gaaaagagaa aatattaacg atagaaaata agataaacaa tcatgccgat agataaaatt
    29761 ccgaagccaa tatatgccac catataagcg aagaagagtc cgggattttt tgctcgccag
    29821 acatacaccg gttccggtgc atttttaacc cgtttatcaa tcgcgcctgc gcgccagcct
    29881 ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa aaacggcaac gacaaaaaac
    29941 tgctgtacat ccataactac tttccttgtt tcttttgtaa ttcaagtaac tgttcgggtg
    30001 taacggcggg atacccctgc gcatcttccg gcacctggtg catagccgga atagggacca
    30061 gcggccctaa aaaacgcggt tcacgtttaa aaatataaag gtcagccagc gcaccgaaac
    30121 gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt cggtgacggt acggcataac
    30181 actgagcctg aatccccata tgcagcgcaa taaataatgc tcgctcacag tggaaacgtt
    30241 gggtgataat aatgaaatca ttagtatcga aaactttgcg tgtacgcacg atggaatcca
    30301 gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg gtcgacacca gcagcgatta
    30361 aatctttgcg catggtcatc ggctcgttat aactttgcaa tgcgttatcg ccgctcagta
    30421 atagataatt taccttaccg ctgttatagg cattaatcgc tccttgaatg cggtagcgat
    30481 aatactgatt aataacgccg gtacgataat attttgctgt gccgagcacc acgccgacct
    30541 ggcggtaggg gagatcctgc aattcgtcgt agatataagg cgcggttttc cagctcatcc
    30601 agcgatcgag gccgagcaca gtcaacagca gcaagccgat caggactaac aggctgagga
    30661 acacgcgctt taacatgcag gagactcgag ctaaggaaaa tttccatcag gctactttac
    30721 ccgctggttt tgcgcaagaa acggcaaact gtttgccggg aatttcgaca ccaggagagg
    30781 gctggcaagc cggacaaggc gttcacgccg catccggcac caggcttaag cgagaagcac
    30841 gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg gaggtggcat cccactgaat
    30901 caacggcgca tcggcttttt ctgccagaac cgcgcgctgg atatccgggt aatcataacc
    30961 cttgagattc aacagaatta acgccccctg caacggcggc agagtcgggt tgaatgcggc
    31021 gttttcagcg tagctgccgc tgaaaatacg accgtcttta cattccagcg cgacaccgct
    31081 tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct gcaatcgccg cctgagaaag
    31141 cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc atcagcagcg ttttaatctc
    31201 cagatctttc ggcccaaagg catccggcag atagtcacgc agcgcgtgtg cctcgcggcc
    31261 cggcaaatga atacgcagat ccagaccgct gttcagttca ttcataaact gacggcagtg
    31321 accacaaggc gtatagttaa cggtgatggc tgcaagcgct ttttcaccac tcaaccaagc
    31381 gtggctgatc gcgctttgtt cggcatgaac ggtttgctgc attgttgcac caataaattc
    31441 catattggca ccaaaatacc aggttccgct cacaccgcgc gcaattgcgc caacattaaa
    31501 attcgacaat ggcgtacgcg cacaggccgc cgccagcgga agtagtgcga atgccagcgc
    31561 gtcttcgtcc agtcccgttg cgctcttcag cgatgagact tgctccccgg tcaacaaagc
    31621 ggggaagtac ttgtctgcca gaataggttc cagtgcagat tgcaagttat ccgcaagttg
    31681 ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct cataatcatg taatgggata
    31741 acagtttacg aacccgttat ggctttatat gtgatctgaa tctcattaat tatgcaacac
    31801 gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt tagcccatta ctgccagaat
    31861 aatcgggaaa aggaacggtg cgatcagcga agtaattatc ccgcataaca ccagcgctag
    31921 cgaactaaat gcaccttcct gataatccag ctcggcgcag cgcgccgtac cgagggcgtg
    31981 cgaggcagtc cccattgcca gaccgcgcgc agctttggta cgaatacgca tcgcattaag
    32041 caatgtatgg ccaaataccg cgccgaggat gccgacgaaa atcacgcaaa ctgcgctgat
    32101 tgccggaata ccgccaatac tgccgccaac cgccattgca ataggcgtgg tgactgattt
    32161 cggcaggatt gacgcggcga tttccggtga agcgcccatc aacaatgcca cggaagtccc
    32221 cgtcaccatc gcaaccacgc tgccgataaa acagatggtg atgatcgatt tccagcgcgc
    32281 gcggatctgg tgtagctgct catataaagg ataggccagc gcgacgaccg ccggttgcag
    32341 cagatcgttt agcacttcgc tacctttaaa gtaactgtcg taagagatgc cggtcagcat
    32401 taaaaaagga atgatcacca ccattgctac cagcaacggg ttaagcaggg gaaacttata
    32461 ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac gtcagcggta atgaccacca
    32521 gatattcgcc atcattcttc tgatcctttc tgacctacga ctttacgttc accgtgtacc
    32581 agttgtgaac tccagctcac caccagaaaa accaccagcg tactgacagc acaggaaacc
    32641 actaccgggc caaactgcgc gcggagcaaa tcaaaatatt gcatgacgcc tacgccaatc
    32701 ggcacaaaca atagcgccat atagcgaatc agtacgtagc accccggatt gacccatttc
    32761 gccggaagaa tttgcaaggc cagcaggaca aacaggatca gcatcccgat gatgctgccc
    32821 ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg catacaggca ggcataaatc
    32881 aggacgaaag cgcgtaaata ttgccagata atgttcagtg tcttactcat aatcaacacc
    32941 ccagatgaga tggattcatc atacaattaa ttcgtaaaac gtgctaccga tcacattaag
    33001 aaatcagcgc ttattttttc gctacccggt attacgcaat gagcagcgaa ctcaggcata
    33061 catcaagcat agagagggat tgcagtttgc agcaatccct ctttgcagac ctttaatccg
    33121 ctgaccatag gcgctaacat agcgcctcat ttttttgcgg gtgatgatat gcgtgtgtta
    33181 ctggcaccga tggagggagt gcttgactct ctggtgcgtg aattgctgac cgaagttaac
    33241 gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg atcaactgct gccggtaaaa
    33301 gtctttcatc gcatttgccc tgagctacaa aacgccagcc ggacaccatc tggtacgctg
    33361 gtgcgcgtgc agttgttagg tcagttccca caatggctgg cagagaacgc cgcccgtgcg
    33421 gtggagttag gtttctgggg cgtggatctc aattgcggct gcccgtcgaa aacggttaac
    33481 ggtagcggcg gcggggcgac gttactcaaa gatcctgaac tcatctacca gggggcaaaa
    33541 gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg tgaaagtgcg tctgggctgg
    33601 gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc aacaggctgg cgctacggag
    33661 ctggtggtgc atgggcggac gaaagagcag ggttaccgcg cggagcatat tgactggcag
    33721 gcgattggcg agattcgcca gcggctgaat attccggtga ttgccaacgg tgaaatctgg
    33781 gactggcaga gcgcgcaaca atgcatggcg atcagcggct gcgacgcagt gatgattggt
    33841 cgcggggcgc tcaatattcc caacctgagc cgggtggtaa aatataacga accgcgaatg
    33901 ccgtggccgg aggtggttgc tttgctgcaa aaatataccc gtctggaaaa gcagggcgat
    33961 accgggttat atcacgttgc gcggattaaa cagtggttga gttatttgcg taaagaatac
    34021 gatgaagcaa cggaattatt tcagcatgtt cgggtgttga ataactcccc tgatattgca
    34081 agggctattc aggcaattga tatcgagaaa ctctaaaaac catagtgatg tttttgacaa
    34141 tgtgttagct ctttatcaat aggccgcacg gttgtttggt ggcgtcaaaa tcgccgaggc
    34201 gttccctgaa ggccggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
    34261 gttacctcgc gcccgacccg gtagccgtaa gggataagcc gagggcaccg cgcagcggcg
    34321 attttgttcg ccagagcccg ggggggcagg gggcggcggc gattggtcgc cccctgcgcg
    34381 ctccttgcgc cagtgattat atgaagcaat gcacatgaca ggagcgcaac aggatgagag
    34441 actgaaatat tctcgttatt ttcggatctg atgacattaa ttaattttaa aatatcatct
    34501 taaatacgcc agtcaccacc agtagcccaa tcaggaaaat aatcaataca gcccagagta
    34561 taattttcat tggcgttcct ttatttgtga gtgctgaata agtataggaa aaaacgaagc
    34621 cctttgccgt tatccataaa cgattaagat aaatcggaag atatcattta cttagtctat
    34681 atttttctgg gtatgtcgtt ttattgctac ttatgtattg ccacgcgaaa ctaaaaaata
    34741 tatcgcaaca cacggtaatc tccgcgcacc gtttcttacc tgattattcc cccatgaatc
    34801 gtgattcctt ttatccagcc atcgcctgtt ttccgctgtt actgatgctg gccgggtgtg
    34861 cgcctatgca tgaaacccgc caggcgttaa gccagcaaac gcccgctgca caagttgaca
    34921 ccgcattacc cacggcgctg aaaaatggtt ggccagacag ccaatggtgg ctggagtatc
    34981 acgataatca gctcacttca ttaattaaca atgcactaca gaacgcaccg gatatgcagg
    35041 tggctgaaca gcgcatttag ctagctgaag cccaggcgaa ggcagttgcc actcaggatg
    35101 gtccgcagat cgacttttcg gcggatatgg agcggcaaaa aatgtcggca gaagggctaa
    35161 tggggccgtt tgctctgaac gatccggccg caggtacgac cggtccgtgg tacaccaacg
    35221 gaacttttgg cttaacggcg ggctggcatc tcgatatctg gggaaagaat cgggcggagg
    35281 ttactgcccg cctgggtacg gttaaagcac gggcggcgga acgcgagcaa acccgccaat
    35341 tgctggctgg cagcgtagcc cgcctgtact gggagtggca aacccaggcg gcgttaaaca
    35401 cggtcttgca gcaaatagaa aaagagcaga acaccattat cgcgaccgat cgccagctat
    35461 atcagaacgg gattacttct tcagttgacg gtgtggaaac cgatattaat gccagcaaaa
    35521 cccggcagca gctcaacgat gtcgcgggga aaatgaaaat tattgaggca cggttaaatg
    35581 cactcacaaa tcaccagact aagtcattga agcttaaacc ggtcgcgttg ccgaaagtgg
    35641 caagccagct tcctgatgaa ctggggtact ccttactggc ccggcgggca gatttgcagg
    35701 cggcgcactg gtacgttgag tcatcgttaa gcactattga tgcggcaaaa gcggcatttt
    35761 atcctgacat caacctgatg gccttcctgc aacaggatgc gttgcactta agcgatctgt
    35821 tccgtcattc cgcgcagcaa atgggcgtta cggcaggcct gacgctacct attttcgata
    35881 gtggtcgtct taacgccaat ctcgatatcg caaaagccga aagcaacttg tctatcgcca
    35941 gctacaacaa agcggtggtt gaagcagtga atgacgtggc gcgggcagcc agtcaggttc
    36001 agacactggc ggagaaaaac cagcatcagg cgcaaattga gcgcgatgcc ttgcgtgtgg
    36061 taggtcttgc gcaggcccgc tttaacgcgg gcatcattgc tggttcccgc gtcagcgaag
    36121 ccagaatccc ggcgctgcgt gagcgggcca atggcctgtt attgcaaggg cagtggctgg
    36181 atgcctccat tcgactcacc ggtgcgctgg gcgggggtta caaacgctga tgatatgcgc
    36241 cttctatact taacgtttat tcagcgttaa gtggagaact cgatggcata ggtcgcgata
    36301 attaccgctt ccgattcggg gatcggcaaa gagtgcgcgt tattactggc gcagcagggg
    36361 tttgatattg gcattacctg gcactcagat gaagaagggg caaaagatac cgcgcgtgag
    36421 gtggttaccc acggcgtacg tgcggagatc gtgcaactgg atctcggcaa tctgccagaa
    36481 ggtgcgcagg cgctggagac actcatccag cgatttgggc gtattgatgt gctggtgaat
    36541 aatgcgggtg caatgaccaa agcgccgttt cttgatatgg cttttgatga gtggcgcaag
    36601 atttttaccg ttgatgttga tggtgcattt ttatgctcgc aaattgcggc tcgtcagatg
    36661 gtgaaataag ggcagggcgg tcgcatcatc aacattacgt cggtacatga acatacgccg
    36721 ctgccggatg ccagcgccta cacagccgct aaacatgcgc tcggcgggtt aaccaaaacg
    36781 atggcgctgg agttggtcag gcataagatt ttggtgaacg cagtcgcgcc tggggcgata
    36841 gccacgccaa tgaacagcat ggatgatagc gacgtgaagc ccgacgcgga gccttcgatt
    36901 cctctacggc gttttggcgc aacgcatgag attgccagcc tggtggtgtg gctttgttcg
    36961 gagggcgcaa attacacgac agggcagtcg ttgatagtgg atggcggctt tatgttggcg
    37021 aatccgcagt tcaacccaga atagcgcagt gaggtgcgtg gatatttcaa tgggttaggc
    37081 cagataagac accagcgtcg catcaggcat ggaacacccg gtgccggatg tggagtgaat
    37141 gcttatctgt ggcaactgct ttacgcctga ttatccggct ttttcttccc gcgccgtttc
    37201 agccaccagc gcacgcccac caccagaacc acgaccagaa tcaaccagac ccagtgcttc
    37261 aaatgctggt cgagattgtg caaccacggc gcaatcacct gaccaccagc gtaaccaata
    37321 gtggtaaaaa tcaacgccca ggcaaatgcg ccgagaatat tcagcggcag aaagattttc
    37381 ggcggcagct ggctggcacc aatcagcgtc gggccaatca cccgaaagcc atacataaag
    37441 cgcgtaccaa tgacaaacag atacggatgg cgttggataa gtttctgtgc ccgctcaatt
    37501 ttatcctgat gtttcgagaa acggcgtaac agcttgccgc caaaccgccg cccgcacaga
    37561 tagagcacct ggtcgccaat catgccacca agcgccacag aaagtaccac cagtgggaac
    37621 tttaatagcc cctgatgtgc cgcaacgcct cccagcaaag tcacggtttc accttccgcc
    37681 aggctaccga taaccagcgc ggcataacca tattgtgaga tgagtgtatt gagatccata
    37741 ggccagtttg tctccctttg gttgcctgta agcatacacc ctgtgaatca ttcacgctga
    37801 aaaaggacat acatcagacg gtgcaaatta catggatgaa caaaaaataa tcacaagctg
    37861 gcttatactt gaactgtcag gttcatgaac tcacaacaag ggggcggtat gagccatgtc
    37921 tggggactgt tttcccatcc cgatcgtgaa atgcaggtga ttaatcgcga aaacgaaacg
    37981 atttctcacc attacaccca ccacgttttg ctgatggcgg cgattccggt gatttgcgcc
    38041 ttcattggca ctacacagat tggctggaat tttggcgatg gcactatcct gaagttatcc
    38101 tggtttaccg gactggcgct ggctgtcttg ttttacggcg ttatgctggc tggggttgcg
    38161 gtgatggggc gggtcatctg gtggatggcg cgcaattacc cgcagcgtcc gtctcttgcg
    38221 cactgcatgg tcttcgcggg ctacgttgca actccgctgt ttttaagcgg tctggtggcg
    38281 ctttatccac tggtctggtt gtgtgcgctg gtcggcacgg tggcgctgtt ttacaccggc
    38341 tacctgcttt accttggtat tccgagcttc ctgaatatca acaaagagga aggccttagc
    38401 ttctccagtt cgacgctggc gattggcgtg ctggtactgg aagtgctgct caccctaacc
    38461 gttatactat ggggctacgg ttatcgtttg ttctagaaag ttctttgacg ttgcattgct
    38521 ggcgtaaatg tcagcaatgc agcatttact cacgattctc ctttgacgat cgcacttttt
    38581 gctcgttatg atggcgtcgc tagcctcagt aaatccttac tccggcggtg cgcaacccgt
    38641 gcgcgtgaac cactatctga atgctcatca tgccgaaatt tcgagtttct ttatttagct
    38701 tggccctgat gctggctgtg ccttttgcac cgcaggccgt tgcgaaaacg gcagccgcta
    38761 ccaccgcttc acaaccggaa attgcctccg gtagcgcgat gattgttgat ctgaatacca
    38821 acaaagtgat ctattcgaac cacccggatc tggtgcgtcc gattgcgtct atcagcaaat
    38881 taatgaccgc gatggttgtg ctggatgcac gactgccgct ggatgaaaaa ctaaaagtgg
    38941 atatcagcca gacgccggag atgaaagggg tctattcgcg cgtacgactg aatagcgaaa
    39001 tcagccgtaa agatatgctg ttgctggcgc tgatgtcttc agaaaaccgc gcggcggcaa
    39061 gccttgcgca ccattatccc ggtggttaca aagcctttat taaggcaatg aatgcgaaag
    39121 cgaaatcgct cggaatgaac aacacgcgct ttgttgaacc taccggattg tcggtgcata
    39181 acgtttcaac tgcccgtgac ttaaccaaac tgctcattgc cagcaaacaa tatccgttga
    39241 tcgggcagtt aagtactacc cgggaagata tggcaacctt ctctaacccg acgtatacgc
    39301 tgccgttccg caatactaat catctggtgt atcgcgataa ctggaatatt cagttaacca
    39361 aaaccggctt taccaatgcg gcgggccatt gtctggtgat gcgtacggtt atcaataata
    39421 aaccggtggc gctggtagtg atggacgcgt ttggcaaata tacccatttt gccgatgcca
    39481 gccgcctgcg tacctggatt gaaaccggta aagtgatgcc tgtgccggca gcagcgttga
    39541 gctataaaaa acaaaaagcc gcccaaatgg cggcggcggg gcagacggca cagaacgatt
    39601 aagtgaattc ggatggcgat actctgccat ccgtaatttt tactccactt cctgccagtt
    39661 tttccgttta ctggttttac cgatccccgg attcatgcta ttggtcggat cattctcgcg
    39721 atagaacttc tgcaacgtct ccggtgcttt atacaaatga ccgacgttat gctcggcagg
    39781 gtactgcgcg ccgcgctgct gtagcagttc cagcatctgt tcttttaacg catgcacatc
    39841 cacgcctttt ttcactatgt aatcctgatg gaagacatag cacataaaat ggccgtaata
    39901 gagcttgtgc accagctggc tgtcgatctc cggcggtaaa tgctcatacc actcggtgtc
    39961 gttacgccgc agagcgatat ccagtgccag aatgtcttcg acttcatcgg aatgcaccgc
    40021 ctgataacga attgctgcgc ccgcagcggc gaaacggtgt aaaaacgctt tgctaccttc
    40081 ctccggcgta cagacaaaga aatcgccctc ggcctgtttg aaataatcca ccagccacga
    40141 tttggcttca ccgacgccat cgcctgccat ttttaacagc agatgatgct cgtatttatc
    40201 gcgccagttt ttcatgcgcg gcggtaaatg gctggggaac aggtgaccga atttttgcat
    40261 tgcacggtca gtaaaatgcg gacggaagaa tttcactttc tccagcatcg catcggtgcg
    40321 tcccttgaga ttaaagaaga acggcatctt gtcggtgcct aacttatcaa tcatcaggaa
    40381 ggtgtctttg ccgtattttt ccgcaatatc gtagatatcc cggtgcatat attccccggc
    40441 aaccggcaga ttttcgaagt tagccagaat atgacggcgg atttcggtca gcacttccgg
    40501 ctggttggtg ccgatataaa acacctgctg atttttttcc gcttcgaagg tatcaagacg
    40561 taccgcaaag acggccagct tcccggcgca accagaagat tcaaataacc gatccggatc
    40621 ggcgttataa cgtgcgggcg tgtcggcttc aatatcacga acgcggtgga catagtcata
    40681 atcgtgggcg tgacgaccat cgtgacgcac atcgtcatct ttgatgcgat cgtcatccag
    40741 cttgctaagg atctgctccg gcgtttcgcc cagatcaatc cccagatggt tcaccagcgt
    40801 cagtttgcca tcttcatcta tacgcgcgaa taacgacatt tcggtatacg ccgggccacg
    40861 ttgcaccagc gagccgccgg agttgttaca aataccgccg atgaccgatg cgcctataca
    40921 cgatgatcca atcaccgaat gcggttcgcg tcccagcggt ttgagggctt tttccagcga
    40981 atagagcgtg gtgcccggat aggccagcac ctgttcgccc ttgccgagaa cgtgcagctt
    41041 gtcgagacgc agggtgctga tgatcacgat atcgcgatca tagtcgttac cgtttggcgt
    41101 cgatccttcg gtcaggcctg tattggcagc ctgcatcaga ataattttgt cggcggtgac
    41161 gcaggctttc agcacccgcc acaattccag tagtgagcca gggaaaacga cagccagcgc
    41221 gtcgccctga ccagaacgga agcccttgcg atagcgggcc gtttttgcgg gatcggtgag
    41281 caggtgtgaa tgaccgacca gacgggcaag ttcattcaaa aaggctttat tatcagttgt
    41341 tgtcatggaa gacattttcc actccttgtg gtggcgaaaa aatatcacaa actagaatag
    41401 cgccttgtat gacaattcag cgagtgaata ttttgaaaat tcagacaata aactgagcat
    41461 tctttttctc tctccatcat gcttatggca aactacggtt tttgcaccat tttcgctgcc
    41521 acgtcgggca acaaaggaag aaaaatccat atgaaatggc tatgttcagt aggaatcgcg
    41581 gtgagtctgg tcctgcagcc agcactggcg gatgatttat tcggtaacca tccattaacg
    41641 cctgaagcgc gggatgcgtt cgtcaccgat ctgctcaaga aaatgacggt tgatgagaaa
    41701 attggtcagc tgcgcttaat cagcgtaggc ccggataatc cgaaagaggc gatccgcgag
    41761 atgatcaaag acggtcaggt tggggcgatt ttcaacaccg taacccgtca ggatatccgc
    41821 gccatgcagg atcaggtgat ggaattaagc cgcctgaaaa ttcctctttt ctttgcttac
    41881 gacgtgctgc acggtcagcg cacggtgttc ccgattagcc tcggtctggc ctcgtctttt
    41941 aacctcgatg cggtgaaaac ggtcgggcgt gtctctgctt atgaagcggc agatgatggc
    42001 ctgaatatga cctgggcacc gatggtcgat gtctcgcgcg atccgcgctg gggacgtgct
    42061 tccgaaggtt ttggcgaaga tacgtatctc acctcaataa tgggtaaaac catggtggaa
    42121 gcgatgcagg gtaaaagccc ggcagatcgt tactcggtga tgaccagcgt caaacacttt
    42181 gccgcatacg gcgcggtaga aggcggtaaa gagtacaaca ccgtcgatat gagtccgcag
    42241 cgcctgttta atgattatat gccgccgtac aaagcggggc tggacgcagg cagcggcgcg
    42301 gtgatggtgg cgctgaactc gctgaacggc acgccagcca cctccgattc ctggctgctg
    42361 aaagatgttc tgcgcgacca gtggggcttt aaaggcatca ccgtttccga tcacggtgca
    42421 atcaaagagc tgattaaaca tggcacggcg gcagacccgg aagatgcggt gcgcgtggcg
    42481 ctgaaatccg gaatcaacat gagtatgagc gacgagtact actcgaagta tctgcctggg
    42541 ttgattaaat ccggcaaagt gaccatggca gagctggacg atgctgcccg ccatgtactg
    42601 aacgttaaat atgatatggg gttgtttaac gacccataca gccatttggg gccgaaagag
    42661 tctgacccgg tggataccaa tgccgaaagc cgcctgcacc gtaaagaagc gcgtgaagtg
    42721 gcgcgcgaaa gcctggtgtt gctgaaaaac cgtctcgaaa cgttaccgct gaaaaaatcg
    42781 gccaccattg cggtggttgg gccactggcg gacagtaaac gtgacgtgat gggcagctgg
    42841 tccgcagccg gtgttgccga tcaatccgtg accgtactga ccgggattaa aaattccgtc
    42901 ggtgaaaacg gtaaagtgct gtatgccaaa ggggcgaacg ttaccagtga caaaggcatt
    42961 atcgatttcc tgaatcagta tgaagaagcg gtcaaagtcg atccgcgttc gccgcaagag
    43021 atgattgatg aagcggtgca gacggcgaaa caatctgatg tggtggtggc tgtagtcggt
    43081 gaagcacagg ggatggcgca cgaagcctcc agccggaccg atatcactat tccgcaaggc
    43141 caacgtgact tgattgcggc gctgaaagcc accggtaaac cgctggtgct ggtgctgatg
    43201 aacgggcgtc cgctggcgct ggtgaaagaa gatcagcagg ctgatgcgat tctggaaacc
    43261 tggtttgcgg ggactgaagg cggtaatgca attgccgatg tattgtttgg cgattacaac
    43321 ccgtccggca agctgccaat ttccttcccg cgttctgtcg ggcagatccc ggtgtactac
    43381 agccatctga acaccggtcg tccgtataat gccgacaagc cgaacaaata tacttcgcgt
    43441 tattttgatg aagctaacgg agcgctttat ccgttcggct atggtctgag ctataccact
    43501 ttcaccgtct ctgatgtgaa actttctgcg ccgaccatga agcgtgacgg caaagtgacg
    43561 gccagcgtgc aggtgacgaa caccggtaag cgcgaggggg ccacggtagt gcagatgtac
    43621 ttgcaggatg tgacggcttc catgagtcgc cctgtgaaac agctgaaagg ctttgagaaa
    43681 atcaccctga aaccgggcga aactcagact gtcagcttcc cgatcgatat tgaggcgctg
    43741 aagttctgga atcaacagat gaaatatgac gccgagcctg gcaagttcaa tgtatttatc
    43801 ggcactgatt ccgcacgcgt taagaaaggc gagtttgagt tgctgtaatg ctttagtaag
    43861 ggcgcgacgt ttgcgccctt tgcctgatgt tcatatctta acttgctgaa ttttcgccct
    43921 ttgtaggccg gataaggcgc tcgcgccgca tccggcagga aacgccggac cgaatatgcc
    43981 tcttaagtat ttatctgatt ctgcaactga atccttccgc tcaggctaac tctgtcatta
    44041 tcaactatgc ttttctctta attcgctgaa aacaacagta aaagaatgag gaaagcatca
    44101 tgccactctt aaagctctgg gcaggttcac tggttatgtt ggcagccgtg agcctgccgc
    44161 tacaggcggc ttcccccgtt aaagtcggtt caaaaatcga taccgaaggc gcgctgctcg
    44221 gcaacatcat cttgcaggta ctcgaaagcc acggcgtacc gacggtgaat aaagtgcaac
    44281 tcggaacgac tcctgtgggg cgcggggcga ttacttccga tgaactggat atctatccgg
    44341 aatataccgg caatggcgct ttcttcttta aagatgaaaa cgatgcagcg tggaaaaatg
    44401 ccgggcaggg ctacgagaaa gtcaaaaagc tcgatgcgga gcacaacaag ttaatctggc
    44461 tgacgcccgc gcctgcaaat aacacctgga ctatcgccgt gcgtcaggat gtggcagaga
    44521 aaaacaaact cacttcgctt gccgacctga gtcgttatct gcaagagggc ggcaccttca
    44581 aactggcagc ctcggcagag tttatcgaac gcgccgatgc gttacccacg tttgaaaaag
    44641 cctatggctt taagctcggt caggatcagt tgctgtcact ggctggtggc gacacggcgg
    44701 tgacgattaa agccgctgcc cagcaaacct ctggcgttaa tgccgcaatg gcttacggca
    44761 ctgacggtcc ggtcgcggcg ctggggctgc aaaccttaag cgatccgcaa ggcgtgcaac
    44821 ctatctacgc gcctgcacca gtggtgcgtg aatcggtgtt gaaggagtat ccgcaaatgg
    44881 cacagtggct acagccagtc ttcgccagcc tcgatgcaaa aacattgcag caactgaatg
    44941 ccagcattgc agtggaagga ctggatgcca aaaaagtggc tgccgactac ctgaaacaaa
    45001 aagggtgggc gaagtaattt cccgtgactt atctacgtat taatcctgtt ctggcgctgc
    45061 tgttgttgct gacggcaatc gcagcggcgc tgccgtttat cagttacgcg cctaatcgtt
    45121 tagtctcggg tgaagggctt catctctggc aactgtggcc gcaaacgatc tggatgctgg
    45181 tgggcgttgg ttgcgcctgg ctgacagcct gttttgtccc cgctaaaaaa ggcagcattt
    45241 ttgcactcat tctggcgcaa ttcgtcttcg tattgttggt gtggggagct ggaaaggcgg
    45301 cgacccaact ggcgcaaaat ggcagtgcgc tggcgcgtac cagcctcggc agaggtttct
    45361 ggctggctgc ggcgctggca ttgctggcct gtagcgatgc catccgccga atctccacgc
    45421 atccgctgtg gcgctggttg ttgcatatgc agattgccat tattccgctg tggttgctgt
    45481 actccggcac gcttaacgat ctctcactaa tgaaagaata cgccaaccgt caggatgtgt
    45541 ttgacgacgc gctggcacaa catctgacgt tgctgtttgg tgcggtgctg cctgcgttag
    45601 tgattggtgt gccgttgggc atctggtgct acttttccac tgcgcggcag ggggcaattt
    45661 tttctctgct caatgtcatt cagaccgtgc cttcggtggc gctctttggc ctgttgattg
    45721 cgccgcttgc cgcgctggtt acggcctttc cgtggctggg gaagctcggc atagcaggaa
    45781 ccggaatgac acccgcactg attgcgctgg tgctctatgc cttgctgccg ctggtgcgag
    45841 gcgtggtagt cggtttgaac cagatcccgc gcgatgtgct ggagagcgcc agagcgatgg
    45901 ggatgagcgg ggcgcagcgt ttcctgcatg ttcagttacc gctggcgtta ccggtatttc
    45961 tgcgcagcct gcgggtggtg atggtgcaaa ctgtaggtat ggcggtgatt gcggcgttaa
    46021 tcggcgcagg cggttttggt gcgctggttt tccaggggct gctaagcagc gccattgatt
    46081 tagtgttgct gggggtgatc ccggtaattg ttctggcggt gcttaccgac gcgctgttcg
    46141 atttgcttat cgcactgctg aaggtgaaac gtaatgattg aatttagcca tgtcagcaaa
    46201 ctgttcggcg cacaaaaagc cgttaacgat ctcaatctca attttcagga agggagtttt
    46261 tcggtgctga ttggcacatc tggctccggc aaatccacca ccctgaaaat gattaaccgc
    46321 ctggtggagc atgacagcgg agtgatccgc tttgccggag aagaaattcg ctcgctgcca
    46381 gtactggagt tgcgccgccg gatgggctat gccattcaat ctattggcct gttcccccac
    46441 tggagcgtgg cgcaaaacat cgctaccgtg ccgcaattac aaaaatggtc gcgggcgcgg
    46501 attgacgatc gtatcgacga attaatggcg ctactggggc tggagtcaaa tttgcgtgag
    46561 cgttatccgc atcagctttc cggtggtcag cagcaacgtg tgggagtggc gcgtgcactg
    46621 gctgccgatc cgcaagtctt actgatggat gaaccttttg gcgcactgga cccggtaacg
    46681 cgcggcgcgt tgcaacaaga gatgacgcgc attcaccgtt tgctggggcg cactattgtg
    46741 ctggtcactc atgatattga tgaggcgcta cggctggcag aacatctggt attgatggat
    46801 cacggtgaag tggtgcagca ggggaatccg ctgacgatgc tgactcgtcc ggcgaatgat
    46861 tttgtccgcc agttttttgg acgtagtgaa ctgggggtgc acctgctttc gttacgtagc
    46921 gtggcggatt acgtgcgtcg cgaagaacgg gcagaaggtg aggcactggc agaagagatg
    46981 acgctacgcg atgcgctctc cctgtttgtc gcgcggggat gcgaggtgct gccggtggtg
    47041 aacacgcagg gccagccttg cggcacgctg cattttcagg atctgctggt ggaggcgtaa
    47101 gcgtatgaag atgttgcgcg atccgctgtt ctggctcatt gctctttttg tggcgctgat
    47161 tttctggctg ccttacagcc agccgctgtt tgctgccttg ttcccacaac tgccacgacc
    47221 cgtttatcag caagaaagtt ttgcagctct ggcactggct catttctggc tggtgggaat
    47281 ttcgagtttg tttgcggtga tcattggcac aggtgccgga attgctgtta ctcgcccgtg
    47341 gggcgcggaa tttcgcccac tggtggaaac tattgccgcc gttggacaga cttttccgcc
    47401 tgtcgcagtg ctggcgattg ccgttccggt gatcggcttt ggtctgcaac cagcgattat
    47461 cgccttgatc ctttacggtg tgctgcccat cctgcaggtg acacttgccg ggctgggagc
    47521 ga
//
LOCUS       DAJTYM010000020        47332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTYM010000020 DAJTYM010000000
VERSION     DAJTYM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OO575_001154"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OO575_001154"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6664195.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO575_001155"
     CDS             complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO575_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6664196.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO575_001156"
     CDS             complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO575_001156"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6664197.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO575_001157"
     CDS             complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO575_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6664198.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO575_001158"
     CDS             complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO575_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6664199.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO575_001159"
     CDS             complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO575_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6664200.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO575_001160"
     CDS             complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO575_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6664201.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO575_001161"
     CDS             complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO575_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6664202.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO575_001162"
     CDS             complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO575_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6664203.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO575_001163"
     CDS             complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO575_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6664204.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO575_001164"
     CDS             complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO575_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6664205.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO575_001165"
     CDS             complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO575_001165"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6664206.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10283..11086
                     /gene="suhB"
                     /locus_tag="OO575_001166"
     CDS             10283..11086
                     /gene="suhB"
                     /locus_tag="OO575_001166"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6664207.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11231..12085
                     /locus_tag="OO575_001167"
     CDS             11231..12085
                     /locus_tag="OO575_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6664208.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12276..13556
                     /gene="csiE"
                     /locus_tag="OO575_001168"
     CDS             12276..13556
                     /gene="csiE"
                     /locus_tag="OO575_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6664209.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13548..14687)
                     /locus_tag="OO575_001169"
     CDS             complement(13548..14687)
                     /locus_tag="OO575_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6664210.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO575_001170"
     CDS             complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO575_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6664211.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15873..17234
                     /locus_tag="OO575_001171"
     CDS             15873..17234
                     /locus_tag="OO575_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6664212.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17231..17749
                     /locus_tag="OO575_001172"
     CDS             17231..17749
                     /locus_tag="OO575_001172"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6664213.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17749..18069
                     /locus_tag="OO575_001173"
     CDS             17749..18069
                     /locus_tag="OO575_001173"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6664214.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18066..18878
                     /gene="hcaB"
                     /locus_tag="OO575_001174"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18066..18878
                     /gene="hcaB"
                     /locus_tag="OO575_001174"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6664215.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18888..20105
                     /locus_tag="OO575_001175"
     CDS             18888..20105
                     /locus_tag="OO575_001175"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6664216.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20202..20624
                     /locus_tag="OO575_001176"
     CDS             20202..20624
                     /locus_tag="OO575_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6664217.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20672..21544)
                     /locus_tag="OO575_001177"
     CDS             complement(20672..21544)
                     /locus_tag="OO575_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6664218.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21556..22650)
                     /locus_tag="OO575_001178"
     CDS             complement(21556..22650)
                     /locus_tag="OO575_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6664219.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22683..23681)
                     /locus_tag="OO575_001179"
     CDS             complement(22683..23681)
                     /locus_tag="OO575_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6664220.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23706..25217)
                     /locus_tag="OO575_001180"
     CDS             complement(23706..25217)
                     /locus_tag="OO575_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6664221.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25240..26222)
                     /locus_tag="OO575_001181"
                     /pseudo
     CDS             complement(25240..26222)
                     /locus_tag="OO575_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26319..29420)
                     /locus_tag="OO575_001182"
     CDS             complement(26319..29420)
                     /locus_tag="OO575_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6664222.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29427..29600)
                     /locus_tag="OO575_001183"
     CDS             complement(29427..29600)
                     /locus_tag="OO575_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664223.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29718..30911
                     /locus_tag="OO575_001184"
     CDS             29718..30911
                     /locus_tag="OO575_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6664224.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31109..32362)
                     /gene="glyA"
                     /locus_tag="OO575_001185"
     CDS             complement(31109..32362)
                     /gene="glyA"
                     /locus_tag="OO575_001185"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6664225.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32689..33879
                     /gene="hmpA"
                     /locus_tag="OO575_001186"
     CDS             32689..33879
                     /gene="hmpA"
                     /locus_tag="OO575_001186"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6664226.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33924..34262)
                     /gene="glnB"
                     /locus_tag="OO575_001187"
     CDS             complement(33924..34262)
                     /gene="glnB"
                     /locus_tag="OO575_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6664227.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34323..35657)
                     /gene="glrR"
                     /locus_tag="OO575_001188"
     CDS             complement(34323..35657)
                     /gene="glrR"
                     /locus_tag="OO575_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6664228.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35647..36360)
                     /gene="qseG"
                     /locus_tag="OO575_001189"
     CDS             complement(35647..36360)
                     /gene="qseG"
                     /locus_tag="OO575_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6664229.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36525..37952)
                     /gene="qseE"
                     /locus_tag="OO575_001190"
     CDS             complement(36525..37952)
                     /gene="qseE"
                     /locus_tag="OO575_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6664230.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38510..42397)
                     /gene="purL"
                     /locus_tag="OO575_001191"
                     /gene_synonym="purI"
     CDS             complement(38510..42397)
                     /gene="purL"
                     /locus_tag="OO575_001191"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6664231.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42656..44212
                     /gene="mltF"
                     /locus_tag="OO575_001192"
     CDS             42656..44212
                     /gene="mltF"
                     /locus_tag="OO575_001192"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6664232.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44209..44745)
                     /gene="tadA"
                     /locus_tag="OO575_001193"
     CDS             complement(44209..44745)
                     /gene="tadA"
                     /locus_tag="OO575_001193"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6664233.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44770..45405)
                     /gene="yfhb"
                     /locus_tag="OO575_001194"
     CDS             complement(44770..45405)
                     /gene="yfhb"
                     /locus_tag="OO575_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6664234.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45614..46462
                     /locus_tag="OO575_001195"
     CDS             45614..46462
                     /locus_tag="OO575_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6664235.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46801..47332)
                     /locus_tag="OO575_001196"
                     /pseudo
     CDS             complement(46801..47332)
                     /locus_tag="OO575_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11282 a        12965 c        11905 g        11180 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtatattaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa
      601 caatactcac cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag
      661 cgtattatcg acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag
      721 tccgaattca gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa
      781 gatattactt aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag
      841 gcggttattt gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa
      901 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
      961 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
     1021 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
     1081 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
     1141 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
     1201 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
     1261 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
     1321 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
     1381 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
     1441 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
     1501 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
     1561 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
     1621 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
     1681 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
     1741 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
     1801 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
     1861 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
     1921 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
     1981 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
     2041 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
     2101 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
     2161 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
     2221 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
     2281 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
     2341 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
     2401 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
     2461 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca
     2521 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
     2581 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
     2641 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
     2701 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
     2761 cagcagagga acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc
     2821 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
     2881 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc
     2941 ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
     3001 gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
     3061 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
     3121 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
     3181 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
     3241 actttacgcg tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca
     3301 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc
     3361 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
     3421 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
     3481 atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc
     3541 cccaggcagc ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag
     3601 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
     3661 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
     3721 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
     3781 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
     3841 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
     3901 tcctgctctg agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg
     3961 gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg
     4021 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga
     4081 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
     4141 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
     4201 tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc
     4261 ggcagcattg tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag
     4321 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg
     4381 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
     4441 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
     4501 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
     4561 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
     4621 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
     4681 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
     4741 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
     4801 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
     4861 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
     4921 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
     4981 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
     5041 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
     5101 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag
     5161 ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc
     5221 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
     5281 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
     5341 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt
     5401 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
     5461 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
     5521 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
     5581 cgggttcagt agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
     5641 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
     5701 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
     5761 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
     5821 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
     5881 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
     5941 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
     6001 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg
     6061 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
     6121 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
     6181 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
     6241 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
     6301 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
     6361 gttccgccag gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
     6421 aacgcaggct cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
     6481 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
     6541 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
     6601 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
     6661 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
     6721 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
     6781 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
     6841 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
     6901 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
     6961 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
     7021 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
     7081 tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa
     7141 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
     7201 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac
     7261 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
     7321 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
     7381 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
     7441 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
     7501 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
     7561 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
     7621 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
     7681 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
     7741 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
     7801 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
     7861 cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac
     7921 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
     7981 ggtcgattgg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
     8041 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
     8101 gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga
     8161 taataccgtt acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac
     8221 ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
     8281 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
     8341 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
     8401 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
     8461 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
     8521 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
     8581 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
     8641 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
     8701 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
     8761 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
     8821 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
     8881 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
     8941 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
     9001 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
     9061 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
     9121 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
     9181 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
     9241 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
     9301 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
     9361 cctacgattc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca
     9421 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
     9481 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     9541 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     9601 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     9661 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     9721 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9781 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9841 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
     9901 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
     9961 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag
    10021 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    10081 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    10141 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    10201 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    10261 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    10321 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    10381 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    10441 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    10501 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    10561 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    10621 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc
    10681 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    10741 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10801 caagctgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10861 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    10921 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10981 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat
    11041 actggcgaac atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt
    11101 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    11161 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    11221 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    11281 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    11341 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    11401 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    11461 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    11521 ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    11581 taaatcaaaa ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    11641 tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat
    11701 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    11761 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11821 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11881 cccgctttta ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa
    11941 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    12001 tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct
    12061 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    12121 caccttaaga catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac
    12181 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    12241 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    12301 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    12361 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    12421 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    12481 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    12541 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    12601 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    12661 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    12721 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12781 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12841 aggaaattgg acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac
    12901 tgtttatggc attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    12961 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    13021 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc
    13081 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    13141 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    13201 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    13261 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    13321 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    13381 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    13441 ttattgcgcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    13501 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    13561 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    13621 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    13681 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca
    13741 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13801 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13861 caaatcgccg agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc
    13921 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13981 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    14041 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    14101 gcagaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    14161 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    14221 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    14281 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    14341 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    14401 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc
    14461 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    14521 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    14581 aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag
    14641 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    14701 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    14761 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    14821 acgcatgttg caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14881 atggcgatga aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa
    14941 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    15001 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    15061 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    15121 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    15181 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    15241 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    15301 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    15361 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    15421 ggcaataaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    15481 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    15541 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    15601 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    15661 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    15721 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15781 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15841 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15901 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15961 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    16021 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    16081 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    16141 tgcgtgtgag ttatgcagat tgtggcaaca gtcgcgcctt tacctgcccg tatcacggct
    16201 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    16261 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    16321 gactgatttt tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca
    16381 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    16441 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    16501 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    16561 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    16621 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    16681 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    16741 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    16801 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    16861 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16921 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16981 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    17041 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    17101 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    17161 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    17221 aggtgatgaa atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct
    17281 ctttcacgaa gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga
    17341 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa
    17401 aggcgtccag ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg
    17461 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcactcgtca
    17521 cttaatcagc aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt
    17581 gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    17641 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    17701 cctggatcaa gcggtgatca cttcccataa cctgagtgta ctgttctgat aaatcgaatt
    17761 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc
    17821 gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17881 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17941 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    18001 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    18061 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    18121 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt
    18181 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    18241 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    18301 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    18361 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    18421 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    18481 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca
    18541 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    18601 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    18661 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    18721 cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc
    18781 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18841 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    18901 cgatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18961 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    19021 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    19081 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    19141 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    19201 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    19261 gctttaccct acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    19321 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    19381 agcgcagcgc agcgcagcgc agatgtaagg tgacagtgat tgaactggcg gcaaccgtca
    19441 tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt acagcgccat cagcaggctg
    19501 gtgtgcgcat tctgctcaat aatgccattg aacatgtggt cgatggtgaa aaagtagaac
    19561 tgacgctgca aagtggggaa acgcttcagg ctgatgtggt gatttacggt attggtatca
    19621 gcgccaacga gcaactggct cgcgaggcca accttgatac tgccaatggc attgtcattg
    19681 atgaggcttg ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg gcaatcactc
    19741 gtcttgataa tggtgcacta caccgctgcg aaagctggga aaacgccaat aaccaggcgc
    19801 aaattgccgc tgccgcaatg ttagggctac cgctaccgct actgccgccg ccgtggttct
    19861 ggagcgatca gtacagtgat aacttacagt ttattggcga tatgcgtggc gatgactggc
    19921 tttgtcgtgg caacccggaa actcagaagg cgatttggtt taatctgcaa aacggcgtgc
    19981 ttatcggtgc ggtcacgctg aatcagggcc gtgagattcg cccaattcgc aaatggatcc
    20041 agagcggcaa aacgtttgat gcgaaactgc tgatagatga gaacatcgcg cttaaatcac
    20101 tgtaatcagg ataattagcg aatatctcaa tgcctggggc gtggcgaggt gcaagagtgt
    20161 gtattacgtt taaatcacat tatcttgcaa agggattggt tatgaacaca ctacgttatt
    20221 ttgattttgg agctgcccgc cccgttttgt tattaattgc ccgtatcgcc gtggtcttaa
    20281 ttttcattat ttttggtttt cccaaaatga tgggctttga cggtacggtc caatatatgg
    20341 cctcgttggg cgcgccaatg ccgatgctgg cagcgattat tgcggtagtt atggaagtgc
    20401 ccgccgcgat attaatcgtg cttggctttt tcacccgtcc gctggcggtg ctgtttattt
    20461 tctacacgct gggtacggcg gtgattggtc accattactg ggatatgacc ggcgatgcgg
    20521 ttgggccaaa tatgattaat ttctggaaga atgtcagtat cgctggcgcg ttcttgctat
    20581 tggcaattac cggaccgggg gcaatttctc tcgatcggcg ttaggaaaga tgccggatgc
    20641 ggcgtgaacg ccttatccgg cattttataa attacagcca ctctacccgc aacgacatct
    20701 ctgaggttgt tgattcccct ggtgctagcg caatgagatc acccccttcc gggcgatgat
    20761 gctcatccgg cgcatggctc atcggttcca gacagaagaa atcaaacgca tatcctttat
    20821 caaacgcagg gtcggaaaca aagatgaaat aacacggtgc aggtggcgtc gtttccatga
    20881 tgatggcata accttcctgc ggctgctcga tgcgggcctg accattccat ccggcaaaac
    20941 cattgttcac ccactgacgc ggcaacggcg cgagctggtt aaaatccagt tcctgcggta
    21001 gctgctcgca aaactcaccc gccagccact gctcccgttc cagccagtaa ccgctcgcct
    21061 gcgcctgaat ccgcgtttgt ggtgacaacg gaaaataagg atgccagccg gtaccaaatg
    21121 gcagcgtctc tgccccttga ttggtgacag agagcgtcac cgtcagcgta tccgccgtta
    21181 aatgaaacgc ctgacttacc cgatagtgat agacaccgct gcgatgttca tacaccagac
    21241 acaaactatc atcgctgtgc gagacacatt gccactcgcc cagccagcca tcgccgtgta
    21301 gatagtgcgc atcccactca acattcggtt gcagttgata ctcacgcccc tgccagacaa
    21361 aacggttgcc gctcacccgg tttgcaaatg gcaccagcgg aaaacatgag gcatcggttg
    21421 ccacaccgct ttttttacca ggacgtaaga gcggcgtcgt atcgcgccag aagccttcga
    21481 ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc gtgggataag gtatagatgg
    21541 tcatccgcta actccttaat ccgggaagtt aatcacaact ttcccgcatt tgccgctcgc
    21601 catcagcaca taggcatcac ctgcctgttc tagcgagaag cgatgggtaa tggcgttacg
    21661 cggccacagc ttccagtcag tcaaatcgtg ggcgcatttt tccatatgga acagactggt
    21721 cacccaggag ccgataatcc gccgttgatg gtgcatcaga tcagcgctga cctcgaattc
    21781 cacttttccg gtttcaccaa tgtaaaccac ccgcccccag tcagcagtgg attgcagtgc
    21841 cagcaagcga cctgcggcat taccggaaca atcgagcgca acatccgcgc caccgtgggt
    21901 aagttcagca attatctgcg gcagaccttc ggtggttgct aaatagccgt gatccatccc
    21961 ccctaactgt tttgccatcg ccagacgttc cggcagcata tcaacgccga tgatccgttt
    22021 tgcaccgcga cctttcgcca gcatcatcgc catcatgccg accggcccca gaccgaccac
    22081 cagcacgtta tcgctgccgg aaacttcgcc gcgcaaaatc ccttcatagg ccgtgccaac
    22141 gccgcaactg ataaacgcac catcttcgta gctcagcgca tccggcagga ggatcagatc
    22201 tttttcttcc gccagcagat attcggcatg accgccgtca cgctgccagc cgtaagccgc
    22261 ttttcctttg ccagtacaag agataggaaa gccgcggcgg cagttcgggc aaaaaccaca
    22321 gccagaaatg tgatacacca gcacgcggtc gccctcttta aaatggcggc agccttgccc
    22381 catcgccaca atctgcccgc acggttcatg accgttgata aatccctggt ataacggttt
    22441 atcgggtgcc gccgccgtgg cacggtgttg atgatagata tagtggacat cgcttccgca
    22501 aatcccggag gatttcattt tgatcagtac ctggttaatc cccggcgtcg gcaccgcaac
    22561 ttcccgcaga tcgacggttg aatttcctag taaataagca gccagcatcg ttttcataaa
    22621 tccctcatta acaatacgat taattttcat ccctgcagca cccgcgccag gggcgttaag
    22681 gtttagcgtt tcgctttact gcttcgctgg gtcagcaaga tattcgccag caccgccacc
    22741 acgatgatga cgccgcgtac cacctgctgg aaaaaggagt taataccgag cagcaccaga
    22801 ccgttaccga ttagcgtaat caccagcaca ccaagcaatg taccgaacag ggaaccgcga
    22861 ccgccggaaa gtgccgtacc gccgaccacg accgcggcga tgacgtcaaa ctccagaccg
    22921 tttgcggcac ctgcgttacc ggaaccgagg cgtgccgcca acaaaatgcc ggtcaccgcc
    22981 gctaataatc ccgaaagggt aaagataaga atgcgcactc gacgaacgtt aataccgcac
    23041 aactgcgccg ccgtggcatt accgccaacg gcaaaaaccg agcgcccgaa ggcggttttt
    23101 cggctaatga acacaaacag cgcaaacaac accatcatga tcagcgcgga tactggcaca
    23161 ccgagaaatt gtccgcccag ccagtccagc acctcgtttt cgtcaatcgg cactggcagt
    23221 gcgttggtca taaacagccc cattccgcgc agagcgctcc acagccccag tgtggcaacg
    23281 aaacttggta cgttaaacac gccgcgcagc accccggcga gcgttcccat cagcgcgcct
    23341 aacagcaaca ccagcagaca agcaatcgcc aacggaactt caaattgcag caaaaatgcc
    23401 aggcacaccg agacaaaagc caccatcggc ccaacgctga catcaatttc accggagata
    23461 ataatcagcg tcatcgccca ggcggcaatc ccaatggtgg cggcatcgcg cagcacgttc
    23521 atctggttat tcaatgagat aaagccaggc gcgttcaggg agaagaccag ataaagaatg
    23581 gcaatcacca ccagcaaacc gatctcatta atatggcgac tgacaaattg tttgagcgag
    23641 acgctcttgc cctgcggcaa tggtaatgac gaagcataca tcttcaggtt cctcggtaaa
    23701 attcatcagt gcacagacag aatggcggac atcagctcat ccacattgac cggcgagtga
    23761 aactcctgcg agaacgtgcc gtgctgtaat aacaggatgc ggtcacacac cagcggcaat
    23821 tcctccactt cactggagat aaacaccacg ctttttcctt cggcagccag ctcacggaca
    23881 atacggtaaa tctgctgttt ggcttcgata tcgacgccgc gcgttggctc gtcgagcaac
    23941 aaaatctggc tggcggcata gacccaacga ccgatcacca ctttttgctg attgccacca
    24001 gaaagcgtgc cgatgggtgt ttcgctactg gcagccttga ccgtcatccg ctgcatcacc
    24061 tcttcggtca ggcggcggat ggtggaccat tgcagcacac cgttggtgct gattttttgc
    24121 cgattggtca gcactgtatt ttcgtcaacg cctagccagg gaatgatccc cgcttctttg
    24181 cggttttctg gcgtatagcc aatgccgcgt ttcagcatgt cgccgtaatc ggggcgcatg
    24241 attttctcgc cgttgataac aatttcgccc tgttcatacg tctccagccc aacaatcgcc
    24301 ttcagcaatt cactgcgccc tgcccccagc aggccagcaa tgccaagcac ttcgccacga
    24361 cgtagcgtaa agctgatatc ctccagcttg ggcttatggc gtaacgcacg gacttccagt
    24421 acggcctgat ccacaatttc ctgaggggca accggcgcaa tatcaacgtg atcacgccct
    24481 agcattagcg acacaatatg atgcgtggag gtgttttcga gcatcacatc gcccgccacc
    24541 tgaccatcgc gcataacggt ggcataggag gcaatgcggc ggatttcttc catccggtgg
    24601 ctgacataaa tcaccgccac gcccagtgcc gacatctttt tcaccgcact gatcaccagt
    24661 tcaacttctg cactggcaag cgagctggtg ggttcatcaa gaatgaccac gcgcggctcg
    24721 cccttcatca cccgtgcaat ttccaccagt tgcttttgcg ccgggcttag cgttgaaaca
    24781 agttgttcag gactaacgtc aacgcccagc gcctgtaagc aacgttgggc atcctgcgcc
    24841 atttgcaggt aatcaatcat gccgttgcgg cgaggccatt gaccaaggca gaggttttct
    24901 gccaccgtca gcccttccac cagacttaat tcctgataaa ccgcacgaac ccccagttca
    24961 gcggcacggc gagtcaacgt agcttcgtca ccttccagtc gcgtctcgcc aatccagata
    25021 tcaccgctat ccgggcgttc gctgccggta agcattcgaa tgagagtcga tttgcccgcg
    25081 ccgtttttac ctaacagcgc acgaacttcg cctttattga gcgtgaagtt aacgttatcc
    25141 agcgcaacga cgccggggta acgcttattt cctgccacca cttttgctac cgggactgcc
    25201 tctgttgccg tgaacatagg gacctctgcg aatcagcgat tagggcagac catcaacgtg
    25261 cgttgccagc cactgtttac catcttccgt tttggtatag agatcgattg gcacctgaat
    25321 cactttttca ccgtcggctt gtttattgat aaccttcaat gtttgcgcga aaacagcatt
    25381 gcccattttc ttaccggaaa tatccactac cgctttcagc acctgattgt tttccagctc
    25441 ctgagcaatt tcggttgtca tatccgaacc gaaaacagca atttttccgg cctgattttg
    25501 attacgtacc gcttttaccg cgccgagtgt cgcaccgccc gactccccca taatggcgtt
    25561 gagatccggc gtggagataa tcagtttttc accaacggaa atcgctttat ctaaaacagt
    25621 cccttcctgg ttagcgacaa tttgcgcgcc gggaacgcgg gattttaata cttcttcaaa
    25681 tcctttacgt cgctgcacac aaacttcaaa ggcttcgcaa ttgatgacgg caattttcgg
    25741 ctggtcaatt ttattggcaa taaaataatc ggcggcagcg ttacccagtt tttaccaaat
    25801 tccagtggat cgccgaccag atacgccgag acatatttat cgacaccctt ttgattaata
    25861 caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta
    25921 ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc cacgagggta
    25981 tcaacaaagg tactttcttt cgaaatatcc ccctgggcgt tagtttcaat taactgtacc
    26041 tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccagc gtaatatccc
    26101 tgggtatcaa ggtatattgc accaatggtc atttctttat ccgcagccct ggcaaatagt
    26161 gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc
    26221 atagtgcctc ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc
    26281 ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata
    26341 aaaggcatca cagtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat
    26401 tcagcgcgag cagactcata ttgcgcaacc tccccgagtc ccgcggacgc cagcatcgcc
    26461 accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga taacaggtca
    26521 ggttgtgaaa cagcaaagaa atccgcctca atactggttt tcgccatctc ttgcgcccac
    26581 tgtttcattt cactaaacag ttgctgtgcg gtttgctgtt cacccagcag tcgcagcgcc
    26641 attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg gatgttaatg
    26701 gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc atcgccctgc
    26761 gcgttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg
    26821 ccttcgctta aattctccgg ataatacagc gcggcatgaa gcagttcgct ggcctgctgc
    26881 ggctctctgg catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactgactg
    26941 gtgaccttcc cttccccgcc ttcccacggg tggaatttac gcgtggcgag aatgtccgcc
    27001 gctttgtctg cctgacccgt gagatgccac aaattgagca gttctgcggt catgtcgtcg
    27061 cgtttcagcg caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggct
    27121 ccacttaact tatccagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc
    27181 tgataagcat tatcaagata acgcgcggcc agctcataat cgtgtttctt attccacgca
    27241 tggatcgcta acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc
    27301 cataaggcaa tggctttgtt gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg
    27361 cgagcaaacc aacactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg
    27421 aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc acgttcggct
    27481 ttcggcagca agctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcgttc
    27541 agcatctctg ccgccagggt gggcattccc cagttaatca gttgcccggc ggtcatcaga
    27601 gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagggctga ttcgctacga
    27661 ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc
    27721 tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcaag
    27781 caaagcactt cctgattggt tgggcaggca cgaagacttt gttggcaaaa atccagaccc
    27841 gcgtcgaagt tatcattacg cgccgccagt cgtgccagac cataatagcc gcctgcttta
    27901 ctattgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga
    27961 cgttcgtaag cactggcgcg aatcaaactt gcttgtccac actgcggttt tttgttcagc
    28021 gcatgtgcac gtttcagcgc ctgactggca tacgccaccg cttgcgggaa atctgcgcgg
    28081 ttatattcca gcatcgccag cgccaggtta cagcgataat ccagcgggtc cagcgccacg
    28141 ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga
    28201 ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgccagtgg cgctttggcg
    28261 acgtccggca acggcaactc ctgcggctga tgttcctgat aactcagtac aatattgccg
    28321 tcggcatcgg agagttcaat ggtcagtctg tcaggattga taccgtgcaa cacgccctgg
    28381 atggcggtcg ctggtgtcag ggccacggca tcatcgagca acgcgttgca tttgccgatt
    28441 tcgcggatcg ccagacggta tccgttcaac ggagagatgg catacagccc ccactcaatc
    28501 ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc
    28561 agagaatgat aaggcaggaa atactgctcg aaacgcttct cttcgtaagc atcaagccag
    28621 gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta
    28681 ttgtcggtca gactcttatc ccacgcctgg ccaaattcac tgtgtcccca actccactgt
    28741 tttttacctg gcgcaatatg gtggttggca acgtgcagca aaccgccatc ttcatcgtga
    28801 caccacgcgc cgacaaaatc gtactgcgat ttttcggcca tatatgaggt tggaacaggc
    28861 acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg
    28921 gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc agtcacatcc
    28981 ggtgggaaaa cgctctgatg cccttcaccc cctttcactg ccgggttgac ccaccacaag
    29041 aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg cgatttccag tgccgcccgg
    29101 tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgccg
    29161 acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag
    29221 gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc
    29281 agcatcccca ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc
    29341 actttatccc atgcgcgatg cacccgaccg cccagttccg gcaggatcat cactttgatg
    29401 tagtcgtttt ccagccacac cgcctgctag gatttcaggg ttttctgctc gctcagcgta
    29461 tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg
    29521 aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc
    29581 cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat
    29641 ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta
    29701 aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca
    29761 aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc
    29821 ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa
    29881 aacgggtggt gaatattgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc
    29941 tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc agtattgagt
    30001 gtcaggttgc taatgcttgt ttaagtccga aaggcgaatt tgagtattta cagattgatg
    30061 taccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat
    30121 tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cgggcaggtt gatccggtga
    30181 ccggcgtatc gcaaaccatg ccgcaagcgc cgtggacaac gccagttgag gtgaagtatc
    30241 tgctggaaga gaagctgggc attcgggtgc tggtcgataa tgactgcgtg atgctggcgc
    30301 tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt
    30361 acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta
    30421 gcggacagat tggtcacacc atcgttaatc cggatggagt cgtctgcgac tgtggacgtt
    30481 acggcagcct ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc
    30541 taaaatcaca accggttaat actcagcttg agcctgaaaa actgacaacc gcgcagttaa
    30601 tcgctgcctg gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg
    30661 ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt
    30721 acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat
    30781 ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc
    30841 aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac
    30901 gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt gatgatcata
    30961 aacgaaaaat tgcctgatgc gctacgctta tcaggcctac acggagattg caatatattg
    31021 aatttgcaag attttgtagg cctgagaagg tgttcatgcc gcatccaaca tgaacaacga
    31081 gcacattgtc agcaaatcac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt
    31141 cgagaacttt acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt
    31201 cacacatcca gccagccagt tctttcgctt cggcttcttt aaagccgcga cgggtaatcg
    31261 ccggagtacc tacacgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc
    31321 tgtttttgtt gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca
    31381 ggtttttatc aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt
    31441 tgtagccgcg ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct
    31501 ggtaagtttt gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta
    31561 cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca
    31621 gctcttcgct accacctttc gccaagatca ggccgccgcg cggacccgcc agggttttgt
    31681 gagtggtggt agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa
    31741 ccaggcccgc aacgtgcgcc atatcaacga acagataagc accgatgctg tcagcgattt
    31801 cacgcatttt cgcccagtca accacgccgg aatatgcaga gaaaccaccg ataatcattt
    31861 tcggcttgtg ttctttggct tgtttttcca gatcggcgta atcgatatga ccggtagcat
    31921 cgataccgta aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag
    31981 tcaggtgacc gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg
    32041 cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag
    32101 cgccgaacag ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc
    32161 aaccgccgta gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac
    32221 cctgcgcctg cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct
    32281 cttcctgacg tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa
    32341 tgttcatttc acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc
    32401 ctttataggc ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac
    32461 aggtcttgac aaaggaattt acgcaaacga ttaccttcag gctacgcaag gctttggaga
    32521 ataaagagct tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact
    32581 tcatcacatt ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat
    32641 aagatgcatt tgagatacat caattaagat gcaaaaaagg aagaccatat gcttgacgct
    32701 caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta
    32761 accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac
    32821 atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac
    32881 gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag
    32941 cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca
    33001 acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat
    33061 ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc
    33121 aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc
    33181 gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt
    33241 ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt
    33301 cagtactctt tgactcgcaa accggatggc aaaggctatc gtatcgcggt gaaacgcgaa
    33361 gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa
    33421 ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta
    33481 atctctaccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca
    33541 ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgaagt tcacgccttt
    33601 gctgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat
    33661 cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat
    33721 ttgagcaaac tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg
    33781 gttggcttca tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac
    33841 attcattacg aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa
    33901 catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg
    33961 cgaatgaccc gagccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc
    34021 gccgtgcgaa taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca
    34081 attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg
    34141 cctttctggc gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg
    34201 gccagtgctt cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc
    34261 atgctattcc ttgaaaaggt tgcctgtctt ttgatctgct aaacgtaaca cataacgcca
    34321 attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct
    34381 gtccggttgc gccccgccat tctcgccgcg tgggtgacgt tgcctttggt gatttgcagc
    34441 agcttacgca agtagttgag ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc
    34501 gtattttcac cctccagcgc ctgctccacc agcgcatcac taatcaccgg agatgaggtc
    34561 agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg
    34621 gtcatcaggc gtttcatcgc atcggtagag aacgcgcgga caaacggttt atgtcgctct
    34681 gccgcctggc gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt
    34741 gccggaattt tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg
    34801 cgcgccatcg cttttgacag atcacggtga gtggcagaaa taatccgcac attgatatca
    34861 atatcgcggt tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg
    34921 acctgtaacg gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc
    34981 gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca
    35041 aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt
    35101 ttgctgttgc gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg
    35161 ctctgaccgt taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc
    35221 aaacgcagca tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct
    35281 ggcgcggatt gctccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc
    35341 ttggtgagga aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca
    35401 tgcgcagtaa gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc
    35461 tgcataccgt ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga
    35521 ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg
    35581 gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc
    35641 gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc
    35701 gtgcggcgta tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat
    35761 atcggtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg
    35821 ttgctggcgc aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg
    35881 gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg
    35941 gcgaacctgt gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg
    36001 ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca
    36061 gctgccgtcg tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc
    36121 acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc
    36181 ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc
    36241 ttctgctggc gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag
    36301 acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat
    36361 attcaccaga cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ctggacatgg
    36421 acagcgcgga gaaaccgggg cgtacatatc gtacgtgaag atttcgagca ctacccgggg
    36481 ccgaactgac aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc
    36541 gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt
    36601 tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc
    36661 accgcccctt ttcgctggtg gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc
    36721 tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa
    36781 ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga
    36841 ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg
    36901 acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc
    36961 accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg
    37021 taatcaagca gttgttcgat cagtttttgc aaattgcagc tgctgctatc aagaatgctc
    37081 accacctctt tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg
    37141 ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga
    37201 tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc
    37261 gagcgtaact cgctcggtcc acagaacgaa acgctattgc ccagagaacg cccttccccc
    37321 agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt
    37381 agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca
    37441 cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc
    37501 tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg
    37561 ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg
    37621 taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg
    37681 cgctggcttt gataaacctt cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag
    37741 ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc
    37801 gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc
    37861 catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt
    37921 aatgagcggg gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc
    37981 ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta
    38041 tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata
    38101 tcagaagttg gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc
    38161 gaaggggctg acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc
    38221 aataaagaaa atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt
    38281 cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa
    38341 cttattagat tgcaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc
    38401 acccattgtc gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg
    38461 gttggtgctt aatgccgcat ccgggctgca aaaccaatga actgatgact taccccaact
    38521 gcttacgcgc attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttccggat
    38581 gccaggagtt gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc
    38641 gaccactttc agtcgtaacc gccgtaatac cgttcggtga accgttcggg ttagccgggt
    38701 aggtttcagt gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt
    38761 ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg cccttcacca tgagagacag
    38821 caatcggcat ttgcgagccc accatcccct gcaacagcag agacgggctt tgggttactt
    38881 caaccaggct gaaacgcgct tcaaagcgat cggaggtgtt acgcacaaaa cgtggccaca
    38941 actcactccc cgggatcagt tcacgcaggt tagacatcat ctggcaaccg ttacataccc
    39001 ccagggccag cgtttgcgga cgatggaaga aggttgcaaa ctcatcgcgt acacggtcat
    39061 tgaacaggat agacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac
    39121 ctccgcacgc gaccagggcg tgaaaatcct ccagtcccgt acgtccagtc agcaggtcgc
    39181 tcatatgcac gtcgatagca tcaaagcccc cacgttggaa agctgccgcc atttcaacat
    39241 gcgagttcac gccctgctca cgcagtacgg caactttcgg acgtgcgcca gtggcaatat
    39301 acggtgctgc cacatcttcg ttgatatcga acgacagttt tacattcagg cccggatcgg
    39361 cgtcgttaga tttcgcctga tgctcctgat cggcacactc cgggttgtca cgcaggcgct
    39421 gcatctgcca ggtagtttct gcccaccaga cacgcaacgt agtgcggctt tcgctgaata
    39481 cagtctgccc gttggcggta atcacaaaac ggtcaccgga aaccgcctgc ccaacataat
    39541 ggacgcaatc cgcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg
    39601 cacgaacctg aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcac
    39661 ccagagaggc gatatccgcg tcaatgccac aatgacctgc aaaggccatt tccgccagcg
    39721 ttaccagcag gcctccatca gagcggtcgt gatacgccag cagcttacgc tgtgcaacca
    39781 gcgcctgaat cgcgtcatag aagcctttca gttggacaac atcgcgtaca tctgccggtt
    39841 tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt
    39901 tgcccaaatc aatcagcagc agtgcgttat cttcggtaca aagctgcggc gtgatggtgt
    39961 gacgtacatc ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt
    40021 cgcgctcttc gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg
    40081 ggatcgtcag gcccagcgcc ggacaaagtt cttcacccac ggctttaacg gcttcataca
    40141 ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc
    40201 gtttgatatc gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac
    40261 gggcagaggc ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt
    40321 caccgtagta gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc
    40381 acggccccac catctgatcg cgcgccacca tgccggttac gctgcggtca ccgatggtca
    40441 ccaggaaagt tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa
    40501 tggtgatccc ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg
    40561 tcatcttcgg cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa
    40621 aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg
    40681 gtgcgcgctc acgcttacac agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca
    40741 gcacgtagcg ttcctgggat tcgttacacc agatttccag cgggctcatg cccggttcgt
    40801 cgcttagaat atcgcgcagt tcaaatttac cgccgcgtcc gccgtcgctc accagctccg
    40861 gcatcgcgtt agaaagaccg cccgcaccga cgtcgtggat aaacagaatt gggttagcat
    40921 caccaagctg ccagcaacgg tcgatcactt cctggcaacg acgctccatc tccgggttgt
    40981 cgcgctgtac ggaagcaaag tcgaggtcgg catcagactg accagacgcc atggaagacg
    41041 ccgcgccgcc gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac
    41101 cgacgttgat ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca
    41161 gcatgatcgg tttgtgataa ccgcgcagct cttcgccatt gtggctgttc actttttctt
    41221 cataagtacg gaagtagccg ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcac
    41281 cgcccagcgg gccttcggtc atgatgtcca gcgcggtaac aatgcgctca ggcttaccga
    41341 aatcttcttc ccacggctgt tcgaagccag gaattcgcag gttggatacg gagaaaccaa
    41401 ccaaaccagc tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc
    41461 cggaaccggt cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga
    41521 ctttcatcag gatgtgcgcc ggttcctgat ggaaattgta gcggcccgtt tcgtggtcag
    41581 caaagtagcg gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga
    41641 gaacgtgatc tggcgtggtt tcgaaagtat ttttgatcat cctgaacagc gatttcggct
    41701 gttgttcacc gtcgataatc cagtcggcgt taaaaatttt gtggcggcag tgctcagagt
    41761 tcgcctgagc gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag
    41821 catcctgcag atagtcaatt tcatcttccg ccagagccaa gccaagacgc aggttagcgt
    41881 cgatcagcgc ctgacggccc tgccccagca aatcaacgct tgtgaccgga gtcggttgat
    41941 ggtgggcaaa caactgctct gcatcatcta aatcaaaaaa gaccgtttcc atcatgcggt
    42001 cgtgcagttc agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat
    42061 agtaagcaac gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat
    42121 cggtcgcttt cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt
    42181 tgccttgcgg ggcgtggctg gcgagtgccg gaccatattt cagcaggcgt tcaagttgtg
    42241 cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa
    42301 tattgtgaac cgggagcctg gcagcctgaa aacgtgccag cagtttgttg attcggaatg
    42361 ccgacagtgc aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt
    42421 cggtgtaccc aagggggggg gaacgggcgt cattataaag aatctgatgc gctgacgaaa
    42481 ccgtttgcgt ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt
    42541 aataagttgc catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt
    42601 cactattatt acgttttttc aagctgggac gcgcacgaca cagagaatta actaattgaa
    42661 aaaattaaag attaattatc tgttcatcgg cattctggca ctgctgctcg cggtcgctct
    42721 ctggccatcc attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg
    42781 gggagagttg cgtgtgagca ccattcatac tcccctgact tataacgaaa tcaacgggaa
    42841 accttttggc ctggattacg aactggcgaa acagtttgcc gattacctcg gcgtaaaact
    42901 gaaagtgacc gtgcggcaga atatcagcca gctgtttgac gaccttgata atggtaacgc
    42961 cgacctgctg gcggcaggac tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg
    43021 ccctacctat tattccgtgt cacaacaact ggtttataaa gtgggtcagt atcgcccacg
    43081 tacgctgggc aacctgacgg cggagcaact caccgttgca ccgggtcatg tggtagttaa
    43141 cgatctccag accctgaaag aaacaaaatt cccggaatta agctggaagg tagacgacaa
    43201 aaaaggctct gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc
    43261 tgattctgtc gccatcagcc tgtttcagcg cgttcacccg gagctcgccg tagcgctcga
    43321 tatcaccgat gaacaaccgg tgacttggtt tagcccgtta gatggcgata ataccctttc
    43381 cgccgccctg ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg cacgcattga
    43441 agagaaatac ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg
    43501 cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat
    43561 tgactggcgt ttgctggccg ctattgctta tcaggaatcg cactgggatg cacaggccac
    43621 ttcaccgacg ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg
    43681 cattacggat cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt atttgcagga
    43741 tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgaa cggatctggt ttgccctcgc
    43801 tgcgtacaat atgggctatg cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa
    43861 agggaatcct gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc
    43921 ctattacagc aagctgactt acggctacgc tcgtgggcat gaagcctacg cttatgtcga
    43981 aaatattcgt aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc
    44041 tacagaagcg gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg
    44101 caaagagaaa tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca
    44161 ttctccctct ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga
    44221 ggattgcgct tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag
    44281 caacgccgcg cactcatccg ccagtattcc ttccgtaatt tccactcggt gattcatacc
    44341 cggatgatgc agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc
    44401 aaagaccacg cgaccaatgc gactgtggat catcgctccg gcacacatta cacatggttc
    44461 aagcgtgaca tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccaccctg
    44521 ccgcagggcc atgatttctg catgtgcggt gggatcatgg cgaccaatcg ggcggttcca
    44581 gccttcgccg attacccgat tgttatgcac taataccgcg ccgaccggca cttcccgctc
    44641 atcccaggca cgtttcgcca gcgtcagcgc gtgacgcatc caatattcgt ggctaaattc
    44701 gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta
    44761 tgctttactc tattccagtt gctggagttc accgcgcggg gttactcgcc aacgatgctg
    44821 acagaaataa agcagcggat tgtcctgatt actgtcgcta tagccactgt acagccgcag
    44881 cggcgtgccg attttgcgct ccagttgtgc gaccttttca tgtcccagac aacgcatcgt
    44941 caatacccaa ccaccatagc cacgctgaat ttggctggcg ataagattaa cccgcggcag
    45001 ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca
    45061 gatatcagca tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc
    45121 ggtaacattg tcgcgaaacc agcgcacgaa atcggcctgc aacgtctgta aacgtgcttc
    45181 gctgtgacca aaagtgcacc cccacagaag cagactcatc ggccagcgtg ccgcacgacc
    45241 ttttatcaat aacgcaatgg ctataatcgg taacaacggc aggacaagta acgcattcag
    45301 cggttggcga cgcagtaaat agcgcagaaa actgccgaac atatcctgct gatgcaatgt
    45361 tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg
    45421 actgaaacat cctgattaat tttcgctact acatagccta acagatagat catcactttt
    45481 ccggcaacaa tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc
    45541 aatggaatat aaaattcact cgcgtgtgtc tcatattgcc tgttgtcgcc actatttcat
    45601 cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag cgttaccaag ggcttgccca
    45661 gagtgataaa aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag
    45721 ctctcagcaa ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca
    45781 aaaactcggc tataaaggtt ttccggcgct taagttggcg ttgagtgaag cgctggcaag
    45841 ccagccggaa tcgccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg
    45901 gctggtcggc gaaaaactga ttaaagaaaa taccgccgct atgtacgcaa cgctaaacgt
    45961 taatagtgaa gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat
    46021 tctgaccggt attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa
    46081 gattggcttc aatgctgccg cactgcgcga tatgcatgcg ctactcgcaa cagtacaggc
    46141 gtcgtcccct gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa
    46201 cctggcggca gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac
    46261 tccgaatgcc ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc
    46321 gacaaacagt gcttcaatct ctgcttgtca cgctcaggga atgttaacgg atttgctgtt
    46381 cattgcgctg attcagcagg atctggaact ggcaccagaa cgtattcgtc atagtgaagc
    46441 gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt
    46501 gttttgagag tttccttatg gcgctgttaa tcactaaaaa atgcatcaat tgtgatatgt
    46561 gtgaaatcaa ataaaaacac atttattttc atagtgatac agcaatgtgt tattgaatgt
    46621 gttgttggat ttatggtgca aaaaaatttt gcataagttc agcaacacaa agaacaccac
    46681 tttctttaac gtcctccgcg ccctaaagga cggggcttta cggcgcaaaa ggcctgtgac
    46741 atacaacatt aacttttcag tcaaccgtag cacgttcttg tatacgccgt gtctgcggtt
    46801 ttatataacc ctggctggaa acctcttata caaagtcgat tcgccaacat catagatgat
    46861 cgccaccttc tggcgagaaa ctcctgatgc aattagtcgc ccggcctgcg cccattattc
    46921 tggtgaaagt ttgggacggc gaccgccaat tcgtccctgt gcgcgagcag cttccagtcc
    46981 tgcttttgtt cgttcaacaa tcagttcacg ttccatttca gccagggcac ccatcacatg
    47041 gaagaaaaag cgtcccattg gtgtgctggt atcaattgaa tcagtcagac tacgaaagtt
    47101 gatgcctcgt tcgcgcaacg cctccaccag cacgacaaga tgccgcatac tgcgccccag
    47161 ccgatccagc ttccagacaa ccagtgtgtc gcctgccgat aatgtcttga gcagtttttt
    47221 cagccccggt ctttcggact ttgtaccact tatcttgtct tcaaaaatca gctcgcatcc
    47281 tgcacagttc aacgcattac gttgtagatc ggtattctgg tcatttgttg at
//
LOCUS       DAJTYM010000021        46009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTYM010000021 DAJTYM010000000
VERSION     DAJTYM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..541)
                     /locus_tag="OO575_001197"
     CDS             complement(<1..541)
                     /locus_tag="OO575_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6664236.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAA"
     gene            complement(752..1204)
                     /locus_tag="OO575_001198"
     CDS             complement(752..1204)
                     /locus_tag="OO575_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6664237.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(1257..2591)
                     /gene="adeQ"
                     /locus_tag="OO575_001199"
                     /gene_synonym="yicO"
     CDS             complement(1257..2591)
                     /gene="adeQ"
                     /locus_tag="OO575_001199"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6664238.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            2766..4532
                     /gene="adeD"
                     /locus_tag="OO575_001200"
     CDS             2766..4532
                     /gene="adeD"
                     /locus_tag="OO575_001200"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6664239.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(4578..5969)
                     /gene="uhpT"
                     /locus_tag="OO575_001201"
     CDS             complement(4578..5969)
                     /gene="uhpT"
                     /locus_tag="OO575_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6664240.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(6107..7426)
                     /gene="uhpC"
                     /locus_tag="OO575_001202"
     CDS             complement(6107..7426)
                     /gene="uhpC"
                     /locus_tag="OO575_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6664241.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(7436..8938)
                     /gene="uhpB"
                     /locus_tag="OO575_001203"
     CDS             complement(7436..8938)
                     /gene="uhpB"
                     /locus_tag="OO575_001203"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6664242.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(8938..9528)
                     /gene="uhpA"
                     /locus_tag="OO575_001204"
     CDS             complement(8938..9528)
                     /gene="uhpA"
                     /locus_tag="OO575_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6664243.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(9603..9893)
                     /gene="ilvN"
                     /locus_tag="OO575_001205"
     CDS             complement(9603..9893)
                     /gene="ilvN"
                     /locus_tag="OO575_001205"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6664244.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(9897..11585)
                     /gene="ilvB"
                     /locus_tag="OO575_001206"
     CDS             complement(9897..11585)
                     /gene="ilvB"
                     /locus_tag="OO575_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6664245.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(11691..11789)
                     /gene="ivbL"
                     /locus_tag="OO575_001207"
     CDS             complement(11691..11789)
                     /gene="ivbL"
                     /locus_tag="OO575_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6664246.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            12354..12443
                     /gene="tisB"
                     /locus_tag="OO575_001208"
     CDS             12354..12443
                     /gene="tisB"
                     /locus_tag="OO575_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6664247.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            12723..13907
                     /gene="emrD"
                     /locus_tag="OO575_001209"
     CDS             12723..13907
                     /gene="emrD"
                     /locus_tag="OO575_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6664248.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(13915..14412)
                     /locus_tag="OO575_001210"
     CDS             complement(13915..14412)
                     /locus_tag="OO575_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6664249.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(14409..14771)
                     /locus_tag="OO575_001211"
     CDS             complement(14409..14771)
                     /locus_tag="OO575_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6664250.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(14761..15108)
                     /locus_tag="OO575_001212"
     CDS             complement(14761..15108)
                     /locus_tag="OO575_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6664251.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            15217..15630
                     /locus_tag="OO575_001213"
     CDS             15217..15630
                     /locus_tag="OO575_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664252.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(15677..17119)
                     /locus_tag="OO575_001214"
                     /pseudo
     CDS             complement(15677..17119)
                     /locus_tag="OO575_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(17154..18869)
                     /locus_tag="OO575_001215"
     CDS             complement(17154..18869)
                     /locus_tag="OO575_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR6664253.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGVEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            19036..19929
                     /locus_tag="OO575_001216"
     CDS             19036..19929
                     /locus_tag="OO575_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6664254.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(19926..20740)
                     /locus_tag="OO575_001217"
                     /pseudo
     CDS             complement(19926..20740)
                     /locus_tag="OO575_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20740..22356)
                     /locus_tag="OO575_001218"
     CDS             complement(20740..22356)
                     /locus_tag="OO575_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6664255.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22651..23325
                     /locus_tag="OO575_001219"
     CDS             22651..23325
                     /locus_tag="OO575_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6664256.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(23364..25025)
                     /locus_tag="OO575_001220"
     CDS             complement(23364..25025)
                     /locus_tag="OO575_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6664257.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(25221..25649)
                     /gene="ibpB"
                     /locus_tag="OO575_001221"
     CDS             complement(25221..25649)
                     /gene="ibpB"
                     /locus_tag="OO575_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6664258.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(25761..26174)
                     /gene="ibpA"
                     /locus_tag="OO575_001222"
     CDS             complement(25761..26174)
                     /gene="ibpA"
                     /locus_tag="OO575_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6664259.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(26168..26240)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            26479..26811
                     /locus_tag="OO575_001223"
     CDS             26479..26811
                     /locus_tag="OO575_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6664260.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(26813..28045)
                     /locus_tag="OO575_001224"
     CDS             complement(26813..28045)
                     /locus_tag="OO575_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6664261.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            28146..29246
                     /gene="cbrA"
                     /locus_tag="OO575_001225"
     CDS             28146..29246
                     /gene="cbrA"
                     /locus_tag="OO575_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6664262.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(29207..30544)
                     /locus_tag="OO575_001226"
     CDS             complement(29207..30544)
                     /locus_tag="OO575_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6664263.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(30619..31767)
                     /gene="dgoD"
                     /locus_tag="OO575_001227"
                     /pseudo
     CDS             complement(30619..31767)
                     /gene="dgoD"
                     /locus_tag="OO575_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(31764..32381)
                     /gene="dgoA"
                     /locus_tag="OO575_001228"
     CDS             complement(31764..32381)
                     /gene="dgoA"
                     /locus_tag="OO575_001228"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6664264.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(32365..33243)
                     /gene="dgoK"
                     /locus_tag="OO575_001229"
     CDS             complement(32365..33243)
                     /gene="dgoK"
                     /locus_tag="OO575_001229"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6664265.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(33240..33929)
                     /gene="dgoR"
                     /locus_tag="OO575_001230"
     CDS             complement(33240..33929)
                     /gene="dgoR"
                     /locus_tag="OO575_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6664266.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            34207..34863
                     /locus_tag="OO575_001231"
     CDS             34207..34863
                     /locus_tag="OO575_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664267.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(34909..35721)
                     /gene="yidA"
                     /locus_tag="OO575_001232"
     CDS             complement(34909..35721)
                     /gene="yidA"
                     /locus_tag="OO575_001232"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6664268.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(35835..36233)
                     /locus_tag="OO575_001233"
     CDS             complement(35835..36233)
                     /locus_tag="OO575_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6664269.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(36473..38887)
                     /gene="gyrB"
                     /locus_tag="OO575_001234"
     CDS             complement(36473..38887)
                     /gene="gyrB"
                     /locus_tag="OO575_001234"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6664270.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(38916..39989)
                     /gene="recF"
                     /locus_tag="OO575_001235"
     CDS             complement(38916..39989)
                     /gene="recF"
                     /locus_tag="OO575_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6664271.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(39989..41089)
                     /gene="dnaN"
                     /locus_tag="OO575_001236"
     CDS             complement(39989..41089)
                     /gene="dnaN"
                     /locus_tag="OO575_001236"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6664272.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(41094..42497)
                     /gene="dnaA"
                     /locus_tag="OO575_001237"
     CDS             complement(41094..42497)
                     /gene="dnaA"
                     /locus_tag="OO575_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6664273.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            42794..42874
                     /locus_tag="OO575_001238"
     CDS             42794..42874
                     /locus_tag="OO575_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664274.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            43104..43244
                     /gene="rpmH"
                     /locus_tag="OO575_001239"
     CDS             43104..43244
                     /gene="rpmH"
                     /locus_tag="OO575_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6664275.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            43261..43620
                     /gene="rnpA"
                     /locus_tag="OO575_001240"
     CDS             43261..43620
                     /gene="rnpA"
                     /locus_tag="OO575_001240"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6664276.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            43584..43841
                     /gene="yidD"
                     /locus_tag="OO575_001241"
     CDS             43584..43841
                     /gene="yidD"
                     /locus_tag="OO575_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6664277.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            43844..45490
                     /gene="yidC"
                     /locus_tag="OO575_001242"
     CDS             43844..45490
                     /gene="yidC"
                     /locus_tag="OO575_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6664278.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            45596..>46009
                     /gene="trmE"
                     /locus_tag="OO575_001243"
                     /gene_synonym="mnmE"
                     /gene_synonym="thdF"
     CDS             45596..>46009
                     /gene="trmE"
                     /locus_tag="OO575_001243"
                     /gene_synonym="mnmE"
                     /gene_synonym="thdF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="in Escherichia coli this protein is involved in the
                     biosynthesis of the hypermodified nucleoside
                     5-methylaminomethyl-2-thiouridine, which is found in the
                     wobble position of some tRNAs and affects ribosomal
                     frameshifting; shows potassium-dependent dimerization and
                     GTP hydrolysis; also involved in regulation of
                     glutamate-dependent acid resistance and activation of
                     gadE; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6664279.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSE"
BASE COUNT        11329 a        12487 c        11648 g        10545 t
ORIGIN      
        1 ccgccgcatt aaagacattg cgccaaccga taagctcgcc taaaaaactg cccaacggcg
       61 cggcaatcac cagcgcaata gaaaccgcgc cgaagatcac cgacagcgcc ttcggcaccg
      121 tacgcggcgg taccagacgc atggtcagcg acgccgacat cgcccagaac ccgcccagcg
      181 ccagccccag acaggcacga ccgattaaaa gcaaactgaa tgagttagca aaggaaacca
      241 gcaagcagga gagcgtcagc aaaacggcaa acaaaataac aacgtagcgg cggtcagtgg
      301 cctgaattgt ctgggtaata aacaaactgg caaacattgc cacaaaggcg gtcacggtca
      361 ccgattgccc ggcaacccct tccgaaatgc ctaaatcctg ggccattggc gtcaacaaac
      421 tgacgggcaa aaactcaacg ataatcagac aggcgacaca aaacgccacc gagaaaacgg
      481 ctgaccagtt cggtcgggtg atggcatccg cgccgcggtt ttcggcaata aattcactca
      541 tggtgttacc cgtggcatgt ttcaggggaa aagccgacca ctttaacatt gaaatgtgtg
      601 acgaatttaa cgtttttgca acttttacgg tgttgcttga gctggagtta agatcgaaac
      661 ggaacagggc aaagattacc ggatgcggcg tgaacgtctt atccacccga caggtcaggc
      721 gcggtctgta ggctgataag acgaaacggt ttcaatgcaa ccagtgaacg ccctcggcag
      781 gctgaaagaa cgcgttatag cgcatctgaa aagcattaat atcctgcatt tcaggttcgg
      841 tttcacgcag aaaacccagc gccagcctga cctgagcatc ggtaatcggc gcggaaaggc
      901 gggctttttg taaaatccgg tgccattgca gcaggttctg ttcgctatca tcgacaatga
      961 caatttgcca gataagcaac acctgcgcgg cgttttgcag gtgcgactca tccgggcgat
     1021 gcaaataacg taaaaattcg tcaccggctg actttcccat ttgatcgacc atcgactcca
     1081 cgggtacgac atcgatattc agccgatcgc tgagacggcg aatcgctttt ctggccccgg
     1141 atgtcagcac ccgaaaacca accacaaaca ctaccgccag cgtggcgagc attatccaga
     1201 tcatgagttc tcctgcgtaa tcagaactca tgatatctgg aaaccctcgc cagttactaa
     1261 tccaccagaa taatcttcaa tgcgaacaga gctgcgacca ccaccacaca caggttcaga
     1321 tcgcgccagc gcccggtgca cactttcatg atgcagtacg acataaagcc gagtgcaatc
     1381 ccttcggtga tcgagaaagt aaagggcatc atcaccgtgg taataaacgc aggcaccgat
     1441 tcggtaaaat catcccagtt aacgcgcgcc aggctcgaag tcatcagcac gccaacaaag
     1501 attaacgctc cagcggttgc gtaaggagga accatcgcca ccagcggtga gaagaacata
     1561 actaacagga acataacgcc gaccacgaca gccgttagcc ccgtgcgacc accgactgcc
     1621 acaccagaag tactttcaat ataggcagta acagacgagg taccgataaa tgcgcctgcc
     1681 accgaactga cgctatcaac atacagcgcc ttattcatat tggggaattt accgttgcta
     1741 tctattaagc ccgctttatc agttacacca attaatgttc ctgatgaatc aaataggttg
     1801 atcagcataa aggagaaaat gataccggcg agttcaagtg ttaacgcgcc gctcaaatct
     1861 acttcaccaa tgacgccgct aatatcaggc ggaatggaat agacgccgct aaaatgaaca
     1921 tcaccgaaaa ataatccaca gcaagacgtc accacaatag aaaccagcac cgcggcatga
     1981 aaatgacgtg atgacaacac ggttataata aaaaacccta aaatacctaa caacacgccg
     2041 tgagaactta aatcgccaat catcaccagc gtgtctttat tggcgacaat aacaccagtg
     2101 ttttttaatc ccattaaggc aataaataat ccgattccac tggtgatacc aatacgtaaa
     2161 cttaatggaa tgttggagat catccagtac cggatacgaa acagcgtgag caaaaatagc
     2221 ccaattgcgc cccagaatat cgcgcccatc ccggtctgcc aggagatccc catcgccccc
     2281 acgaccacga acgcaaagaa ggcgttcagc cccattgccg gagccagcgc cacgggtaag
     2341 ttagcaaata tccccatcgc aatactgccg ataccggcaa tcaaacaggt ggtaacaaac
     2401 accactttcg ggtccatttg tgccgcgccg aggatttgcg ggttcacaaa aacaatgtac
     2461 accatggtta aaaaagtggt catccccgca atcaattctg tgcggacggt ggtcccatgc
     2521 tgaggtagtt taaataatcg cgaaagcgtc cctgattcat tactcacgta atcggtattg
     2581 tcattattca tttttttgtc cattggagga gatttaatcc cttgcttttg taaaacaaat
     2641 gacaacaacg gttcagtgat aattatcaca tttcaattgc acattaatag atattcttta
     2701 ataatctcgc gacgtttctt tatgataaat aataatcaaa ttgataaaat caaaatgaga
     2761 aaaatatgaa taattccatt aaccataaat ttcatcacat tagccgggct gaataccagg
     2821 aattgttagc cgtttcccgt ggcgacgctg ttgccgatta tattattgat aatgtctcta
     2881 ttctcgacct gatcaatgcc ggagaaattt ccggcccaat tgtgattaaa ggacgttaca
     2941 ttgccggtgt tggcgcagaa tacgctgata ctccggcttt gcagcggatt gatgctcgcg
     3001 gcgcaacggc ggtgccaggg tttattgatg ctcacctgca tattgaatcc agcatgatga
     3061 cgccggtcac ttttgaaacc gctaccctgc cgcgcggcct gacgaccgtt atttgcgacc
     3121 ctcatgaaat cgtcaacgtg atgggagaag ccggattcgc ctggtttgcc cgctgtgccg
     3181 aacaggcaag gcaaaaccag tacttacagg tcagctcttg cgtgcccgct ctggaaggct
     3241 gcgatgttaa cggtgccagt tttacccttg aacagatgct cgcctggcgg gaccatccgc
     3301 aggttaccgg ccttgcagaa atgatggact accctggcgt aattaacggg cagaatgcgc
     3361 tgctcgataa actcgatgca tttcgctacc tgacgctgga cggtcactgt ccgggtttgg
     3421 gtggcaaaga acttaacgcc tatattgctg cgggtattga aaactgccac gaaagttatc
     3481 agctggaaga aggacgccgg aaattacaac tcggcatgtc gttgatgatc cgcgaagggt
     3541 ccgctgccag caatctcaac gcactggcac cgttgatcaa cgaatttaac agcccgcaat
     3601 gcatgctctg taccgatgac cgtaacccgt gggagatcgc ccatgaaggg catatcgatg
     3661 ccttaattcg ccgcctgatc gaacaacaca atgtgccgct gcatgtggca tatcgcgtcg
     3721 ccagctggtc gacggcgcgc cactttggtc tgaatcacct cggcttactg gcacccggca
     3781 agcaggccga tatcgtcctg ttgagcgatg cgcgtaaggt cacggtgcag caggtactgg
     3841 tgaaaggcga accgattgat gcgcaaacct tacaggcgga agagtcggcg agactggcgc
     3901 tgtctgcccc gccatatggc aataccattg cccgccagcc agtttccgcc agcgactttg
     3961 ccctgcaatt cacgcccgga aaacgctatc gggtcattga cgtcatccat aacgaattga
     4021 ttacgcactc ccgctccagc gtctacagcg aaaatggttt tgatcgcgat gatgtgtgct
     4081 ttattgccgt acttgagcgt tacgggcaac ggctggctcc ggcctgtggt ttgctcggcg
     4141 gctttggcct gaatgaaggc gcgctggctg cgacagtcag ccatgacagc cataatattg
     4201 tggtgatcgg tcgcagtgcc gaagagatgg cgctggcggt caatcaggtg attcaggatg
     4261 gcggcgggct gtgcgtggta cgtaacggcc aagtacaaag tcatctgccg ttacccattg
     4321 ccgggctgat gagcaccgac acggcgcagt cgctggcgga acaaattgac gccttgaaag
     4381 ccgccgcccg tgaatgcggt ccgttacccg atgagccgtt tattcagatg gcgtttcttt
     4441 ctctgccagt gatccccgcg ctaaaactaa ccagtcaggg gctatttgat ggcgagaagt
     4501 ttgccttcac tacgctggaa gtcacggaat aataaaaaaa gcccggcgtc atgccgggca
     4561 aaagtcacca gttacgttta tgccactgtc aactgctgaa tttttttctc gcggcggatt
     4621 ttgcgttctt ccattaccgc cactatcgcc atcaggcaga tacaaccaat cgcggcgata
     4681 tccagcgcgg cgaaggtgcc tgcccagccg gtaaggccga ataccggcgt cccatcggca
     4741 atcattccca gacctaactt ggcaaagctg tcaccaatca ggtaagcaaa ggtgccttta
     4801 ataccatcgg cagcgccaat cgctttttta ggtacaaagc caacagcagc cacaccaatc
     4861 aacaattgcg ggccaaagac caggaaaccc aacgcaaaga gagaagccag atagatatat
     4921 tcgttactgg catgttgata cacaccgagc gtggcgataa tcagcgccag cgcgatgcag
     4981 gccaccaggc cacggcgacc gttcgccagg tcagagagcc agccccacag cagcgtaccg
     5041 accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg ccaccgcttt agagagtttc
     5101 agttcctgga acgcgtatac ggttgaccac tggtcgatac caatacgtac cacatagagg
     5161 aaaatgttgg cgaagcacag cagccagatc actttgtttt tcagcacata ctcaacaaag
     5221 atctgccact tggtcatatc ggtagattct gtctctttgt cctcttcgct gatctcctcg
     5281 ccgaacagtt cttcagcttt gccgaggcca taagattccg gggagtcgct gccgtaacgc
     5341 aggccgataa aaccgacaat cagcgcgata atcgacggga agataaacat gccgatgaca
     5401 tggccatcga acaggtaatt tgccccgaac agcgccacac ctgctgcgcc tgcaccgcca
     5461 aggttgtgag aaatattcca gaaaccgagg aatgtcccgc gtttacgacg cggcgtccat
     5521 ttggtgatgg tggagtaact gcacgaaccg ccggtactct ggaaaaagcc gcttaaggcg
     5581 tagaaggcaa tcatcaggaa caggctaacc gagccgctgc ccatactggc actgaagccc
     5641 agcatacaaa tggcagagag gataagcatg aacggcagga attgtttggt gtttttgccg
     5701 tcggcgtagt atgaaaccaa cgttttaccc acgccataag tgatggagaa acccaggccg
     5761 atcatcccca gctgcgtcat gctcaacccg taggtcgaaa tcatatcgtt ctgcgcgatg
     5821 ttaaagttct tgcgaatcag gtacatcgtc aggtagccga taaagaccac caggtaggat
     5881 tgcatgaacg gtttgaacca cattttgcgc cgcacttcga gcggaaggtc cagggtcggc
     5941 ttgcgaactt ggtttaggaa agccagcatg ggttactcct gaaatgaata cctgcgtgag
     6001 gcatgcattg taaaaatcag cagcaaatca ggcctgagat agcgtccaag aaaaacctgg
     6061 aaaatttctt agttttgccg gatatgaagt gaaaaggtga gatgcatcac gcttcacgcg
     6121 gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga aatcccggcg gcgatagaga
     6181 taaccacaaa aaatccgctc cagtgccagg tatcgagcac tttcgccagc ggccaaccag
     6241 caagcgacgc ccccagataa gcaaacaagc cgacaaaccc cgtcgccgcc cctgccgcct
     6301 ctttgtggga acactctgcc gccgccatac caattaacat ctgcgggcca aagacaaaaa
     6361 aaccaatggt gaagaagcag gttgcctgca tcacgtagct ggcaaatggc atcagccaca
     6421 gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa attcatcggc ccacggttgc
     6481 cgttaaacaa tttgtccgag ccccaaccgg ctaccagcgc accgataaat ccgcccagtt
     6541 caaacatcgt cactgccgta ttcgccgtga ccagatcgac gcccagcgtc tcggacatat
     6601 acaaattgcc ccagtcgttg atcgccgccc ggaccacata gaccagcaca tagcaaaacg
     6661 aaagcagcca gatatacgga ttcagcaaca catatttggt gaggatctct ttacgcgtca
     6721 accctgcccc ttcttgttgt tgagcaattt ccagcgcgtc gtgccgccat tcaccgaccg
     6781 ccggtaaacc taacgcctgc gggcgatcgc gtagccgcca gcagagaaaa atccccacga
     6841 ctatcgccat acaaccagca atcatcatcc cggcacgcca gccgtaatgc agcgcgctcg
     6901 ctgccatcac aatgggaatg agtgcgccgc cgacgttatg cgccgtgttc cataatgccc
     6961 accaaccgcc gcgctcggta cgtgaatacc aggccgttaa cagacgcgca cacaccggtg
     7021 aaccccagcc ctggaaaaag gcgttcagca cccagagcac ggcaaacgcc catagcgacg
     7081 tcgagaagcc aaacagaatg ttcatgatgc ccgtggcgat aagccctatc cccataaaat
     7141 aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa cttcgacacg ccgtaagtaa
     7201 tgtaaaacag ggtcgctaac aggccgatat cgctacggct gagcacgccg ttagcaagga
     7261 tttctggtac cgcggcgtta aaacttttcc gcgtgaagta aaacagcgcg taacccagcc
     7321 agatggtcag cagaatatgc cgacgccagt agcgataacg ggcatcaatt tcatgtttat
     7381 cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg caacatcatc gaaccttaga
     7441 cataacgttg aggtagagaa acgctgacac gcgtgccgtg cagacaggaa atgtgtaatg
     7501 tgccgcccag cgccgttacg cgctcgcgca ttccggtgag gccaaaacct tgttgcccgg
     7561 aacccggcgg caaaccgctg ccatcgtctt caatcaccag catcaaccgt tcatcctgct
     7621 gccagccttg cagcgtaacc gcgctggcat cggcatgttt cacaatgttg ttcagccctt
     7681 cctggcagac acgaaacagc gtcacgcgct ggttttcgct taatgctgat tcatcgatac
     7741 gccattcgag atggctgaca atgccgcgcc cttccagctc catttcccgc atcagtgagc
     7801 ggatggcctg ctccagggtg agatcatcca gctggcgcgg acgtaaccgt cctaacaaac
     7861 ggcgcaccgc gtcgtaaacg cccagcgaca gttgttcgat gagctgcccg ctctgcttca
     7921 cactggcgtt atctgccgcc agccgctgaa caatgcccgc ctgagtacga atagcagtga
     7981 tggtctgacc gatatcgtcg tgcagctcac gcgccacgtc gcggcgcacg ctctcttcgg
     8041 tttctaacaa acgttcagcc agatgctgat tgcgcgccag ttccttttgt agcgactggt
     8101 taagttcacg caaccgctgg atgcctgcgc ccagtaacaa ccctgtcaga ctttgcacca
     8161 gcaacgagag taataaatcc accagatgat cgcgccaggt ttgactggcg atcagcgcga
     8221 tggcgttcat caacgtcgca atcagcgctc cttgccagcc atagtgccag gccagcgcga
     8281 taatcggcag cgccagacag aatggcgtaa agcgcgacag ttcgtccggc aatcccaact
     8341 ggagccagag actgataaca aacagcagca agtaccagac cagatgccgc ccgcgccagt
     8401 tgatgggctg agaaaccagt gacgggccga gcggcagcca ggtgttattg gcgagatagt
     8461 gccagaacac cagacatatc ggggccagcg tcagaccgcc agttaaagtc agcaacagcg
     8521 cattccacga ctctttgccg tgccaaagcc agggcagcga ctgcaacaac gccgccgccg
     8581 ttaacgccgc accctgtaac agcaaggtgc gccagtcacg ctgatggcga tagcgcgaga
     8641 tcagcgctac gggcagtaac gtcagcaaac taccgatcat caataacgga aaatgggtta
     8701 aaccgaccgc ctgcgttagc cagtaaatca gcagccactc cgcacccagc aatacgggcc
     8761 agtatccgcg cgggcattgc agcattagcc ccagacgcag accaaacgga aataacagca
     8821 ccgccatatc agggcgctca accagatgca ggctgatact ccataggcaa aaccatgcgg
     8881 cagagaagat aaaaaagcag gcaataacgg taattaagcg ggagaacaac gtcttcatca
     8941 ccagccatca aacatgcggc gcgccagctc tacgtcgtta ctgacgccca gtttttccat
     9001 cagattggcg cgatggacgt gtaccgtttt cggtgacaag cccagttcgg cggcaatctc
     9061 tttcaccgcc attccttgcg ccagtttttc cgccacctgg cgttcacgtt tggtcagcgg
     9121 gtcctgacga ccggatgcca gtttaatggc aatatccggc gtcagataac agccgcccgt
     9181 ggcaaccgta tgcaccgcag caatgagttc atccgggcta cagcgtttgg aaagaaagcc
     9241 gcgtgccccc gcgttaagcg cctgctcaac cagcgcagga ctgtcgtgaa cggagagcat
     9301 aatcgtcgcc atacctttcg gcagctggct tagcagctcc agaccggaga tatcgggcat
     9361 ggagatatcg caaatacaca cctgcacacc gcgccccggc agccccgcca gcgcctcgcg
     9421 ccccgaacca aactcggcaa ctacctgcaa atcaggttcc agccccagca gctgcgcaaa
     9481 gccggagcgg acgatgaggt gatcgtctat aagggcaacg gtgatcatgg tcttgtcctg
     9541 gcgggtaaaa aaaaacgcgc ttaccttaac gataagcgcg atgttgttca agccttgagc
     9601 ggttactgaa aaaacaccgc gatcttgtta aacatcgtcg gatcggactg attacgctgc
     9661 actttcacga catcttccag cttagcgatt tggcttatca tctgctccag acgctggtcg
     9721 tcattgacca gtagccagat atggcttttg tcgctgtcct gaatcggcag acaaagaatg
     9781 ccttcaacgt taaaagcgcg gcgggcaaaa aggccacaaa cgtgggtcat tacgcccgga
     9841 tggttgcgaa cggtgagctc cagaattacg ttgttatgag ttgtgttttg catggcttat
     9901 tcccccacca tttcagtatt cgccgcacct ggcggcacca tcgggtaaac tttttcttcg
     9961 gcatcaatgc gcacatggat cagcgccggg ccagggcgat tgatgatttc ctgcaatgca
    10021 gcctgcggat cggcttcgtt attcaaatca caggtttcga ggccgaatcc ggcggcaatc
    10081 tgcataaagt tgattttgcc cggataggtg gcggcaaaaa cgccttgctc gtagaacaga
    10141 ctctgttgct gatgcaccag ccccagcgct tcgttgttca tcagaatgat tttgacatcc
    10201 agctgatttt cactggcggt cgccatctcc tgaatattca tcatcaggct gccgtcgccg
    10261 gagaaacaca acactttgcg atccgggttc gccagcgccg cgccaatcgc cgcaggcagg
    10321 ccaaaaccca tcgtgcccag cccaccggag gtcagccact ggcgtgggcg attgagcgga
    10381 taagcttgcg cggtccacat ctgatgctga cccacatcgg tggtgataat cgcattgtca
    10441 tcgacacagg cggcaacggc gttgatcagg ccgtaatggc ttaatggatc gcacgctttc
    10501 gggattggac acgggaattc gcgctgcaaa tccgctacca actggtgcca ctctgcacgc
    10561 ggttgcgctt ccacccgcgg gatcaattgc gccagcacgt catcaacatc cgcctgaatc
    10621 gccacatgcg gctgcttgat tttacccagc tctgcgcgat cgatatcgac atgaataatt
    10681 ttggcattcg gacagaactg ctcggttttg ccaatcgccc ggtcatcaaa acgcgcaccg
    10741 agcacaatca acaaatccgc ctcctgcaaa atatagttgg tactgcgcac gccgtgcatc
    10801 cccagcatac ccagcgacaa cggatgcgct tttggcagca tgcccagcgc cattaaagtc
    10861 atggtggtag gcagttgcgc tttctccgcc agttcacgca cccgcgcagg cgcattgatc
    10921 acaccaccac ccagataaag caccgggcgt ttggcagcgt taatcattgt agctgcgtca
    10981 cgaatgcttt cttcgctaaa ggcgggtgca acggcttttt ctgccaccgc gggctgagct
    11041 tcaatctcaa aaaccgccgt ttgtacatcc ttaggaatgt ctatccacac cgggctaggg
    11101 cggcctgatt gcgcaatgcg gaaggcatcg ctcatgacct gtgggagctc ttcgatatgt
    11161 ctgaccagat agttgtgttt ggtgatgggg atagagatgc cgtaggtgtc cacttcttgg
    11221 aaggcgtcgg tgccgatcat cgaggcggga acctgaccag tgatgcaaat cagcgggatg
    11281 gagtccagcc gcgcatcagc aatggcggtc accaggttag tcgcacccgg cccgctacag
    11341 gccatacaga ccgccggttt accgtcggtg cgcgccattc cctgagcgat aaaccccgcg
    11401 ccctgttcat ggcgagccag aatatggcgg atttgcgtgc tttggcttaa ggcgtcgtaa
    11461 acaggcagga tagaaccgcc cggaatgccc gtcacaatct taatgccctg ctgttccagg
    11521 aaatgaacga taaattctgc gccggtaaag cgcttacgcg tcgatgttgt gcccgaactt
    11581 gccatgctcc agtccttttc ttctgggccg actttccggg aggtgcttaa acgaaaaacc
    11641 ccgcccggtt tgcgccggcg gggttttgga atcgtgtgtt gttccagtcc ctacggcgca
    11701 ttgccgacga ccaccaccac acgcacgacg accactgcgg cggatggcgc agttggtagt
    11761 agttttgcgt tgaacatgga agtagtcatt ggggaccttg ttggttttgt gtttaacaat
    11821 atttatacaa gcacagcttt acaggggaga caatggaaaa tttttcagca agggaaaatt
    11881 gaggggttga tcacgttttg tactgaattg cagataacaa aaaaccccgc cggagcgagg
    11941 tttcgtcagc cgcctgcggc tggtaaccgc aaagcacact gtattatgtc aacactgaaa
    12001 gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa ttttgacgta tttagtgcat
    12061 agttgagtat cgttcacagt ttgcgttttg tccaaatatt actgtttatt tatacagtaa
    12121 gcttctataa tatcactgta cgcaatgtgt tatgcggggg ccgcatcgtt acccggcgca
    12181 ctaagtcctg gctgaaacgg gtggtgccgt cagcgcctta accccgcgtg agcacactgt
    12241 gttatgtcaa caagcacaac gtttctcctt gagataccgc gtgcacaaca gctggcaaca
    12301 ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc acaggagacg cgtatgagcc
    12361 tggtggatat cgccattctt atcctcaaac tcattgttgc agcactgcaa ctgcttgatg
    12421 ctgttctgaa atacctgaag taattcagat tcaagtcgca ccaaagggga gcgggtaacc
    12481 gctccccttt tatatttagc gtgcgggttg gagtcggatg cgatgctgac gcatcttatc
    12541 cgccctacca tccctcccgg caacatttat tgccgctttt gtttacatat tctgccgcta
    12601 aacaattccc cattcctggc gtatatctgg ctaacattca tcaatgtgat agattcctct
    12661 cccgcattta tgggaatgcg tagtgactta ttctaattat ttttataaaa gcatccgtga
    12721 taatgaaaag gcaaagaaac gtcaatttgt tattgatgtt ggtattactc gtggccgtcg
    12781 gtcagatggc gcaaaccatt tatattccag ctattgccga tatggcgcgc gatctcaacg
    12841 tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct gctgacttac ggtgtctcac
    12901 agctgtttta tggcccgata tccgaccgtg tcggtcgccg accagtgatc ctcgtcggaa
    12961 tgtccatttt tatgctggca acgctggtcg cggtcacgac ctccagtttg acggtattga
    13021 ttgccgccag cgcgatgcag gggatgggca ccggcgttgg cggcgtaatg gcgcgtactt
    13081 tgccgcgtga tttatatgaa cggacacagt tgcgccatgc taacagcctg ttaaacatgg
    13141 ggattctcgt cagtccgttg ctcgcaccgc taatcggcgg tctgctggat acgatgtgga
    13201 actggcgcgc ctgttatctc tttttgttgg ttctttgtgc tggtgtgacc ttcagtatgg
    13261 cccgctggat gccggaaacg cgtccggtcg atgcaccgcg cacgcgcctg cttaccagtt
    13321 ataaaacgct tttcggtaac agcggtttta actgttattt gctgatgctg attggcggtc
    13381 tggccgggat tgctgccttt gaagcctgct ccggcgtgct gatgggcgcg gtgttagggc
    13441 tgagcagtat gacggtcagt attttgttta ttctgccgat tccggcggca ttttttgggg
    13501 catggtttgc cgggcgtcct aataaacgct tctcaacgtt gatgtggcag tcggttatct
    13561 gctgcctgct ggctggcttg ctgatgtgga tccccgactg gtttggcgtg atgaatgtct
    13621 ggacgctgct cgttcccgcc gcgctgttct ttttcggtgc cgggatgctg tttccgctgg
    13681 cgaccagcgg cgcgatggag ccgttcccct tcctggcggg cacggctggt gcgctggtcg
    13741 gcggtctgca aaacattggt tccggcgtgc tggcgtcgct ctctgcgatg ttgccgcaaa
    13801 ccggtcaggg cagcctgggg ttgttgatga ccttaatggg attgttgatc gtgctgtgct
    13861 ggctgccgct ggcgacgcgg atgtcgcatc aggggcagcc cgtttaagcg cacgtcaccg
    13921 cagcatcgtc atcagctcca tgggagaacg atgctgcttt atcagatcac gcatcacccg
    13981 catatgcggt gcggagtaag aataaaacgc ctgatagccc gcacaaagca cgctgtctgg
    14041 cgtctcctgc cgagcgtgcg ggcacaggcg atgccagtcg cacgcctggc agtcacggcg
    14101 cggttgcggg ttttcgctgc cacaccattt ttgtaaggtg gcgtcaaaaa gctcaatcga
    14161 gatacgactg atatctgccc gcacccaaca ctcaaacagg gcaattaaaa atttccccca
    14221 cagctctgct gtcaccgaag cggccgtcag atcgccccgg tcatcaaatt caagataggg
    14281 aatgagctgt acggagcgga tgccccgctg tttgaaattg tgataaaaat cggcaggttg
    14341 cagagcatcc ttgtactcta caaccagctt atgcctgtcc tcttcagcat caatgacctg
    14401 acttcctgtc atgcgactct ctcaataaat acgataagtt gatgtatatg cgttacagca
    14461 aacagtattg cgagagataa caccgcgagg gagatcaaaa atttgtcacg gactacgtga
    14521 aattgggaaa aatcgctatt cgtgacatcc attaaattgc gattacgggt gtagtgccag
    14581 aggatcagcg ccacgatggc gaggatgcca atcgaaatcc agaataacat gcccgcctgg
    14641 tgccagttgt gtttgatagc caacgccatc aacgcgccgt agcccagcat ggtacgaaac
    14701 cacgccagtg atgtgcgctc cggctgcagc cctgggtcgg caatgcgtct ggctttacgg
    14761 ctatccggca tacaacacca gtcccataac gatcaccgcc acaaccatca atattaagct
    14821 gatgattaat aagctgttgg tgtagggcag gtcctctttc agacgcatcg ctttttcatt
    14881 gcgtaaccag cgcaggtagc catacattgc taacccgccg gaaaacagac acaacaacaa
    14941 cgccagcagt tcacgaatga cgggcgtggc gaaatctggc gcaagttgat caagcccaac
    15001 ccccgccgcc agaaatccta gtgcggtacg gatccacgcc agaaaagtac gctcattagc
    15061 cagcgagaag cggtaatcgg gcgcttctcc aaggcgggaa attttcattg ttgctccttt
    15121 gcaatttcac taacgctcta tctttagcgg ttttttttaa gaatttcgtc cgcccgtttc
    15181 ctgatatgct tttgccatcc gctacaagga ggtcagatgg gtatcatagc gcaaaataaa
    15241 atcagttcat tgggcatgct gttcggtgcc atcgctttaa tgatggggat tatccacttt
    15301 tcattcggcc ctttttccgc accaccgcca acgcttgaaa gcatcgtggc ggacaaaacg
    15361 gctgaaatta aaagaggtct tctcgccgga ataaaaggcg aaaaaattac cactgtggag
    15421 aaaaaagagg acatggatat cgataaaatc ctcgatcaaa gcggtatcgc actggccatt
    15481 gctgcgctac tttgcgcatt tattggcggt atgcgtaagg aaaatcgctg gggcattcgc
    15541 ggcgcgctgg tttttggtat cggcatcgtg tgcagcattt tgctgatatt cctgattttt
    15601 tccttcctga ccggcggttc gctggtttaa gcagcctgaa taatgcccgc cgggtgatca
    15661 tcagcgggca ttatcgtcag aatttctgtt ttttctcctc caccttcact ccttgcgtcg
    15721 gtaggccggt gtcatagtcg cgtaccactg gcgaatagcc atcttgtggg cgtgggcgaa
    15781 acgcccccat ccagcgcggt cgtgcatctt tacgccacgg acgcagactc cattggtaac
    15841 tgcggaacgg atcgcgaatt ttgtccatgt aatccaataa ggcgtcatgc attttgctgc
    15901 gaacgtctgc aaaacggata tcatcgatca ggttatgcat ttcatttggg tcattacggc
    15961 gatcgtaaag ttcatcactg gtgaagaggt tgagcaccag tttaaagtca tccgtcaccc
    16021 agcaacgcac cggaataaaa ccgccaaagc tgtcatgctc aatctcgtag cggttaaatt
    16081 ccaccatcac gccgcgtggc tctttcacgg caaggatatt ttcccccggc agaatctctg
    16141 gtttttcaat atctgccagc gccatcattg tcggcagtaa atcaatatga ctgactggcg
    16201 tatcgacctg tcgccgctcc ccttgcggcg aacggatgat cagcggaata cgggtgatgt
    16261 cgtcatacat cgccgctcct ttactgatca gcttatgtgc gcccatcatt tcaccgtgat
    16321 cggaggtata aatgacccac gtattttcac gttgctctgg cgttaaggca ttgatgacac
    16381 gtccgatttg gtcatcaaca aagtcattgc aggcaaaata gagcggatgg tgataaagcc
    16441 cgtcatcacc gactggcgat ggcatcgcct gcgcccataa gcggtgatgt tccggtttgt
    16501 tcgccaggtc atcctgtgat ttctcgccaa gttcgtagta aaaatcagcg tatttctcta
    16561 aatactccac cggacaggtg aacgggtgat gcggctcatc atacgaaacc accatcagga
    16621 agggttcctc ggcgcgcacg ggctattgca gaaaatctac cgctcgattg ctgatgcggt
    16681 gcgcccaggt gaaggtttcg tcaatatggt tcgcctgtaa atcttcgacg ctgtttaggc
    16741 cattgcgcca caggctgatc tctttttccg tcagttcgct aagatagttc gccccatcaa
    16801 accagtaatc agcatcccac tccggcggac actcgccagt gccgaaatag tcatgaccgt
    16861 cgagatgcca tttgccgatg taacaggtgt gatagccggc atccttaaag tagcgcccca
    16921 tagtggagat gtttttgcct ggcgcgacgt tgttggtcca cgggccggac tggttagcgt
    16981 agataccggt gaatagtccg gcccgtgcag gcgtacaaac cggtgaacaa gtgtaggcgg
    17041 aattaaagcg aataccttcc gccgccagac tatcaatatt ttgcgtattc agcggtttac
    17101 cgctatagca accgaccata ttggtggcct gggtatcggt caattggggc gtttcatagg
    17161 ctatccttcg cgtcgggagt aaaggttacg gctgggtcgt gacgatgcca ccagctgtca
    17221 aaaatcaggt agacaatcac tacggcagcg atgaaataac tgatcatcgc taaccagcgc
    17281 gtaccgtagc cgccgaattc agccagcccg acatagacgc caatcatcgc gaacaggata
    17341 ccaattgacg cgatcttgac gtttttccac ggtttcatgt ccaccgcaaa cgcatctttg
    17401 aaggtgaacg gcgtggcgcg cggtttgata aaaccgatca ccagcatcac gaccacgttg
    17461 atgcagaacg tacaggccag cacatagagg aaatggaagt cgaacttcac cagatagttg
    17521 atggtgatgt agctgattat gccaatcccc atcgccactt ttgccgccag cgccgggatg
    17581 cgcggaaaga aaaagcccat aatgatgatg gtaaccagcg gcacgttgta aatgccgttg
    17641 agctgtttca tccagctata cagcccctgc ggcgcgttcg cgatccacgg cgctaccaaa
    17701 accgaaacga tagcgataaa gaaaccaaat ttgcgcccga cggtgaccag ctgctgcggc
    17761 tcggcattct ggttaatgat gcgacggtaa atacccatac tgaataatgt actggcgcta
    17821 ttcagaaagc cgttgaaggt actgatcacc gcaccaaata acaccgcgcc gaagaaaccc
    17881 tccagtggca ctggcagaac gttattgacc agcgtcgggt aggccatgtc ggctttcggt
    17941 agatcctgat acagatgaaa cgcaatcaac cctggcagca ccagtaccag cgggtcgagc
    18001 attttcagca ccgccgttaa cagcgccccc ttttgccctt ccgccaggct ttttgacgcc
    18061 agcgtgcgtt gcacgatgcc ctgatttgta caccagtaaa aggtgttcac cagaattaaa
    18121 ccggtaaatg ccgcgccaat cggcaaggga tcggtcgggc caccgactga gtttaatttc
    18181 tcggcgtgaa cggtggtaat ttgctcaacg ccctgcataa agctgccctt gcctatcgcg
    18241 atcaggccaa ataccggcac catcaacccg ccgataacca gcccaatacc gttgatggag
    18301 tccgccactg ccattgcgcg cagtccgccg atcaccgcat acaaaattcc cgccagacca
    18361 agcaaaatta ccagtagcca gatagccgca ccgtgagaaa tctgtagcga ttccccgacg
    18421 tgaaacaggc tgttgagcgc caacgcgccg gagtagagaa caatcggcag aaagcagacg
    18481 ccggtggcaa tgaggaagca gaagtcgatg ataatacgcg tcgttttatc ataacgttcc
    18541 tccagaaaat cggggatggt ggcaatgccg cgcttcagat agcgcggtaa aaagattagc
    18601 gcgaggaaga tcagcgtcac cgctgaagtc acttcccagc ccatcaccga catgccgctt
    18661 ttgtaggcct gcccggaaag accaaccagt tgttccgtgg aaaggttggt taacattaac
    18721 gaagcggcaa taaccggcgc ttttagtgaa cgtccggcaa gaaaatagcc ttgttgcgat
    18781 ccggtatctg ttttgcgaac cttccaccag gtgatcaccg ccaccagcag cgtaaaaccg
    18841 acaaaactca agatttgtaa cgaattcatc tcatagccct tatttatgtt tatgtcccgg
    18901 acgcatgaat atagggagag gtaaaatgcg tcgggtcagg cagtcagatt cttaaaatac
    18961 aaacgtcgta tccctgaacg gattgttttc tgttaagttc aggttgtgag cataattcgc
    19021 cggagggata ttaaaatgaa tggaaaattg caaagttcag atgtaaaaaa cgaaactccg
    19081 tacaatattc ccttattgat taacgaaaat gtgatctcca gcggaatttc tctgatctcg
    19141 ctctggcata cctacgccga cgagcattac cgggtgatct ggccgcggga caagaagaaa
    19201 ccgcttatcg ccaactcatg ggttgcggtg tataccgtac aaggatgtgg gaaaattctt
    19261 ttaaagaatg gcgaacaaat aactctgcat agcaactgta ttatattttt aaagccaatg
    19321 gatattcact cttatcactg tgaaggttta gtctgggaac agtactggat ggaatttacc
    19381 cccaccagta tgatggatat ttccgttggt cagcaaagcg ttatttataa tggcgaaatt
    19441 tataatcagg aactcaccga agttgctgag ttaataactt caccagaagc aataaaaaat
    19501 aatctggcag tcgcttttct gacgaaaatt atttatcagt ggatttgtct tatgtccgca
    19561 gatggtaaaa aagatccaca acggcggcaa attgaaaaat taattgccac tttacatgcc
    19621 agtctgcaac aacgctggag cgtagctgat atggctgcca cgatcccctg tagcgaagcc
    19681 tggttgcgtc gtctgttttt acgctatacc ggcaagacgc cgaaagaata ttacctcgat
    19741 gcgcgtctgg atctggcgct atcgctatta aagcaacaag gaaactcggt tggcgaagtc
    19801 gctgatacgc tcaacttctt cgactccttt catttcagca aagcctttaa acataaattt
    19861 ggttatgcgc cgtcagccgt gctaaagaat acggaccagc acccaacgga tgccagtcca
    19921 cacaattaac gtaactctgg ccagtacgct ttgttggcct ccagcaactc atccagaata
    19981 gctttggcga ccgaggcgct cggtaccgtt ttcgacaacg taatcgcctg ccacaggtgc
    20041 tgatatgaac gctgttccca ggcgtccacg accagttttt ccaccgccac ttgctgactc
    20101 attaaccctt tctgaaactg cgggatatcg ccgaccacca gtggttcagg ccgtagtagc
    20161 gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg cgccatccgg ctttcaatac
    20221 cgattggcat gtcacagatg ttgaggattt tcgcattcgg gcgcagacga cgcgtggctt
    20281 ctgcgacaat tgctgccggg ttggagtagt tgagcatcta ggcgtttggt gaatattttt
    20341 ccatataatc caccagttcc aggacgccgc caatggaacg catgccgtac gctattccgc
    20401 cgggtccgca agtttcctga ccaacaacac cgtggcgcag tgggattttt tcatccagtt
    20461 cgcgcatcgg gtacttgccg acgcggatgt gcgccatgac aaaatcaacg tcgctgaacg
    20521 ccacttcagg atcggtggtg taactaaacg caatgtccgg cgctttttct ttaaggatga
    20581 ctttacaggc ttcggcaatc acttcctgcc gcgcgccatc gttatcataa aatttcagtg
    20641 cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa cacgatgcct ggcgtaaagg
    20701 tgcttccacc gcctgcgaca accactgaga atttggtcat tatactgcct ccgtaatggc
    20761 aacattttct gctgattgat gagaattaat taagctatcg agctgttcac gcaattggga
    20821 tacatgcaga ccaatgatca cctgaatggc atcgccacta cggaagacgc cgtgcgctcc
    20881 cagcttttta aagacttcgt catccagcgt ttgtgacatg tcatgcagtg caatacgtag
    20941 acgcgtcgcg caattgttaa tgctggagat attgccgacc ccgcccaggg cttgcaggat
    21001 accggcagcc tgatccagct ctttttttgg ctctgcagcg gtggtttggc ctcgcgaggc
    21061 tttgtattcg gcttttgagt agagtttcac ttccgcatct tcacgtcccg gcgtgcacat
    21121 gttgaactgc agaatcaggg tgcggaaaac cacgaagtac agcagggtaa agcacaaccc
    21181 aatggcgatt tgggtcagca tcatatccgc gtggttgctg aacatcggga tccagttttg
    21241 cggtaaaacc tggtcaatca gacctccgcc catgttgccc accacaccaa agagatacat
    21301 tacggtcgac attgaggccg ccagcacggc gtgtaccgca aacagcaacg gtgaaatgaa
    21361 caggaaggta aattccagcg gttcggtaat tcccaccagc atggcggtta aggttgcggg
    21421 aatcagcaag cccgccactt ttacccgatt ttccggtgcg gcagtgaagt acatcgctaa
    21481 agaaatgccc acggcaccaa agatttttga gttaccgtgc agggcaaaac cgccttccgg
    21541 gaacaacgat ttcagtggct cggcgctcag actgaactct tgcagatgct gcgcccagta
    21601 catctgaatg ccgccttcaa cagctgccgg accaaagata aacggtccgt agatgaagtg
    21661 gtgtaaaccg gttgggatca gaatacgttc gaggaaggtg taaacccaca ccccaagcgc
    21721 acccgccgaa cgcaggaacg cttgcagaga ttcaatcccc atttgtactt ttggccagcc
    21781 gagcaatgtc agccaggcac aggggatcat caccaggaag gcgataatca ccacataaga
    21841 cgtcccctgg aaaatgccga gaaaaaccgg cagtttttta tcgaacagac ggttatgcag
    21901 cgccgtcaca atgccggaaa tgataattgc gccgataatg ctggtatcga gggttttaat
    21961 cccggccatc attgtcagac cgctacctgc caccgcgtcc tgagtgaaat cgacgccgaa
    22021 gtagcttccc caggtcattc ccatcgcatt gatgaaatag ttccaggtca ggaaactcac
    22081 catcaccgcc agacaagcac gtccctgcgc ttgcttagca aggccaatgg gtaaaccgac
    22141 agcaaaaatc agcggcatat tacggaatac cgtccaaccg ccctcttcaa taatgtgtac
    22201 gatttgcgcg aataaactgt tcggatcggt cagtgattcc ccgacaaaca tcgggttttg
    22261 cagcaagatg gcaagaccca ccacaatccc ggcgaaggga aacagcagca ctggcgtgaa
    22321 catcgcgccg ccaaagcgtt gaatttgact gagcatttcc acatcctctt ttctcaattc
    22381 tgtaagtagg aagcctgatt ggttatgcgg cagacctgga gcgaggatag gaagatgcga
    22441 tcggcagaac atgacgaccc aatgggattc gtgatcgctt tcatgctttt cgttgggcgg
    22501 tagagatcta cgttttcgcg aaaaagtaga catctgtgag cacaggtaca cagcatcgaa
    22561 cacatcttta aaaaaaagat ttttttcaat cgattaagca gaacttgtgg gcgcattacc
    22621 cgtgcttgca ggcaaaaaag agagctagag atgatctaca aaagcattgc ggagcggtta
    22681 agaattcgac ttaattccgc ggatttcacg ctaaacagcc ttcttcccgg tgaaaaaaag
    22741 ctggcggaag agtttgcggt atcgcggatg actatccgta aagccattga cctgctggta
    22801 gcgtgggggc tggtggtccg ccgccacggc agcggcactt acctggtgcg caaagatgtg
    22861 ctgcatcaaa ccgccagcct gaccggactg gtggaggtgt taaaacggca gggaaaaacg
    22921 gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc ctccggccat tgccagccag
    22981 ttacggattc aaatcaacga gcagatctac ttctcccgtc gcgttcgttt tgtggaaggg
    23041 aaaccgctaa tgctggaaga cagctatatg ccggtaaaac tgttccgtaa cctttcgctg
    23101 caacatctgg aagggtcgaa gtttgaatat attgaacaag agtgcgggat tttgattggc
    23161 ggtaattatg aaagcctgat gccggtgctc gccgatagac tgctggcgcg gcaaatgaag
    23221 gtagcggaac acacgccact gctgcggatc acctcgttgt catatagcga gagcggggag
    23281 tttttgaatt attcagtgat gttcagaaat gccagcgaat actaggtgga gtaccattta
    23341 cggcgactcc acccggaaaa aagttaaccg atactccaga agagcaccgc cagtaattgg
    23401 ggggtgataa tgcgcaggaa catcactaac ggatagacag tggcgtaaga gagcgccgcc
    23461 gcaccactgg ttggatgaag attattagca aacgccagcg ccggaggatc ggtcatggaa
    23521 cctgccagca tcccgcacat ggtcaggtaa ttcattttgg ctaacatccg cgccagaatg
    23581 ccaacagtaa tcagcggaac ggcggtgatc agggcaccat aaccaatcca gcttagccct
    23641 tcgccattga ccagggtatt cacaaaatcc ccaccagatt tcagaccaac gaccgagagg
    23701 aacagcacga tccccagctc ccgcagcgcg aggttggcac ttggcggcat aaaccagtac
    23761 agcttgccga tactgccgat acgcccgagg atcaacgcca taatcagcgg accgcccgcc
    23821 agccccagtt tcaacgcggc cgggaatcct ggcacaaaga cgggaataga acctaacaat
    23881 acgccaagcc cgatgccaat aaacactggc agcatctgaa cctgttgcag tttttgctgc
    23941 gcattcccca gcacattggc aacggcatca attgcggacg gacgccccac caggttgagg
    24001 atatcgccga actgcaggct gatatcgcca ctggcgacca gttcgacccc ggcacggttc
    24061 aggcgcgaga taacaacgtc atagcgttct ttaaagtgca ggtcgcgaat acgttttccg
    24121 agcacgtttt cattggtgac caccacacgc tcgacgcgca aatcagtgcc tttcgtagac
    24181 agcgaggtat cgacctcctg accaatcacc agttgcgcat tatgtaaatc cgctggctgc
    24241 cccaccagat gcagcaaatc gcccagttgg ataatggtat ctggcgaagg tacttttagg
    24301 gtttcttctc gtttcagacg cgagcagata attttgtcgc cgttgagaat cggtacatct
    24361 ttaatggcta aatcatgcag gttagggttc tcaacgcgaa tattgatagt cttgatcagc
    24421 gcgccgccat tggtgcgtga agactcgtgc tgctgagctt ctgtctcgac attgacgcgg
    24481 aaaataaccc gcaacatcca catggtgaac aaaatcccgc aaatgccgaa tggatacgcc
    24541 atcgcgtaac tcatccccat ctgatcgacc atttccattg gtgtacccag gtcgcgcaag
    24601 atctgctgcc ctgcccccag cgctggcgta ttggttaccg caccggagaa aatccccagc
    24661 actaccggca gtggaatatc aaacagttta tgcaggatgg cggtaaccag accaccgatg
    24721 atgacgatca gaacagcaaa caggttgagg cgtaatccgg agacgcgcaa tgaggcaaag
    24781 aagcccggcc ctacctgaat cccgatggta taaacgaaca ggatcaggcc aaattcctga
    24841 ataacatgca gcatatcgct acttaatgtc atccccgcct gagaaacaaa atggccgacg
    24901 atgatcccac caaacagcac gccgccaata cctaatccta tgccgcgaaa tttgacgttg
    24961 ccgataaaca aaccgacgac tgccaccaaa gccagaatac tgaccgttaa tgctatatca
    25021 ctcatcgtcc atttctcttg agataacatt ttaattgcta cggattatgg ctgaatcggt
    25081 gaagcagatt tgccttatag cgcacattat cacgggaaag aacgtgccga aatatcttaa
    25141 acagtcgaaa tttaacgcat tgttgaagaa gaaagacaaa aaaagcccca ccagaatggc
    25201 ggggcaaaga gaatagctag ttagctattt aacgcgggac gttcgctgat agcgatacgc
    25261 tgcgctgcga tgggttcagg ctcattacga attaaatcaa tatgcagtaa accgtttacg
    25321 aaggttgcgc cagagacttc catattttca gccagcgtaa aactcaggct aaatggctga
    25381 ttcataagcc cttgatgcag ccattttttc tcttcttttg gctgctccgg cgtgcctttt
    25441 acgctcaggc gcgtgccttc cagttgaatc tctaaatctt cctgacggaa acctgccagc
    25501 gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa tgttgtacgg cgggaagctc
    25561 tggctttcac cggcgttttg cagtgcgttg gccagtttgt caaaaccgat ccattgacgc
    25621 atcagtgggg ataaatcgaa gttacgcata gtcatttctc cttctaagaa gcgagtaagt
    25681 acctgcaaat ccgaagattc gcatatgctc cctgacggcg agcatggaga tgtcaggccg
    25741 cgccaggcgg ccttagggaa ttagttgatt tcgatacggc gcggtttttt cgcttccgga
    25801 atcacgcgtt cgagatcgat atacagcaaa ccatttacca ggttagcccc acgaacatga
    25861 atgttctcag ctaactggaa tttgcgttca aagttgcgtt cagcgatgcc ctggtacaga
    25921 taggtgcgct ctttttgttc gtcggcgtga gcacctttca ccaccagcag attatcctgg
    25981 gcggtaattt ccagttcgct ctcagcaaaa ccagccacag cgatagcaat gcggtaatgg
    26041 ttttcgtcta ccagttcaac gttatacgga gggtagccgc cattactctg gctctggttg
    26101 ttttctaagt ggttaaacaa acggtcaaat ccaatagcag aacggtaaag cggggataaa
    26161 tcaaagttac gcataatcaa tagctcctga aatcagcgag aatgtaagac cttccacaat
    26221 ggacaggtca ggtagccaga acacccatca ggcgcgtcct catcgactac gatgtaaaat
    26281 gggtctggaa atgaactttt caagccttat cagcggactt ttttttgcag tttatggtct
    26341 attgcataga ctgagggggc agcacgaaac gttaaagtgc gatagccgcc acagtgcgac
    26401 gtaatgatga tgttattttc agcaagggat cgctataact catcaggcca acacccacga
    26461 tcaacagatg aatttattat gataagaaat gtgttgttag cgttcatgat atgcagcgga
    26521 atgacattac tcggaggatg ctccagcgtg atgtcccata ccggggggaa agaaggaacg
    26581 tatcccggca cccgcgccag cgccaccatg attggcgacg atgaaaccaa ctggggcact
    26641 aagtcgctgg cgattctcga tatgccgttt accgctgtaa tggacacgct tttactgccg
    26701 tgggatgtgt tccgcaaaga cagctcggtg cgctcgcgcg ttgaaaaaag cgaagccaac
    26761 gcgcaagcca ccaatgcggt tattccaccg gccagaatgc ctgataactg atttagcgat
    26821 cagtttcggt gatccacagc tggccacctt ccatccacgc cagccattga ccatccggcg
    26881 agaagaccac ggcgtccgca gaaggtggat tggcgtggtt ttcggttaaa tactcaacct
    26941 cgccgctttg cgcatgggca caaacaattc gattatccag cacaaagccc aaccattctc
    27001 ctgacggatg ccagttaaat gcagactgaa tatccgtttt gttatgggtt aacttgcgcg
    27061 gttcgccgcc ctgtggcgag ataagccaca gttgcacaat gccgttatca tcacgcatta
    27121 aaaacgcgat ttgcgtaccc tgcggattac agcgcaccca gtggcgcggg acgttgacta
    27181 accccggata agcccgatga tgggtaaagg ttaaacgtcg ctgcacgacg ccacgcggtg
    27241 gcgcgggtag ggttgtttcc gttccactta acggcgcatc acctgccgat ttccagctag
    27301 cttcatcttg cggtaactca acgataaata gctccggcac tttctcgccc tttggcgaaa
    27361 gtgtgtcgcc aataaacgcc agcgcgtgat ttcctaccca tccttcttca taagcacgat
    27421 tgatttcatc actgccaggc tgcggcgtgg gcgtggtttt actcaccagc acacaccagt
    27481 ggctaccgct gtattcacgc ggatgttgct tttgtacgtt gaccgggcca aacggtgcag
    27541 caaccccgac gtttcgcaaa tccagcgccg gatcgagttg atgcattaca tggtcgttat
    27601 aggtaaagct ccccctttca ccgttcgggc taaagacatg cacatggctg ccaccgcgca
    27661 gcgctcctgg cgtatacggc gcggtaatat ccattgcatc gagattgctc accttgccgc
    27721 cttcagcaat gactccgcgt cgatgatgga agtcgtaatg ccatgtttca tcaggatttt
    27781 cagggccgtg aataaagaca tatttctctg acttcggatg aacggtcacc acgccgacgt
    27841 gtgcgccctg tgacgcgcga tagataacct cgacctcgcc tgtatggata ttcacacgct
    27901 caatggtctc gccggtaaac gacgccccag aaggacgcac gtcaaatacc agccactggc
    27961 tgtcgggcgt ccaggtattg gtatttgtat tggtattggt attggtgagc aggtgattac
    28021 ggggagcaaa ggttatctgt ttcatggcgc ggtcctgata caaaacggtt cccgccatca
    28081 tacttcacaa ggacttcact ttcagacaat agtcttttgc catcgtgatg aatgtgagtg
    28141 aaaagatgga acatttcgac gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg
    28201 cacgaaagtt agccggcaaa atgcaggtga tcgcgctgga taaaaagcac cagtgcggta
    28261 ctgaaggttt cagcaaacct tgcggcggtc tgctggcacc ggacgcgcag cgatctttta
    28321 ttcgcgatgg actgacgctt cctgtcgatg tgatcgccaa tccacagatt ttcagcgtca
    28381 aaactgtcga cgtcgccgca tcgctcacgc gtaactacca gcgaagctat atcaatatta
    28441 atcgccatgc tttcgacttg tggatgaaat cgctgatccc cgccagcgtt gaggtttatc
    28501 acgatagcct gtgccgaaaa atctggcgtg aggatgataa atggcatgtc atttttcgtg
    28561 cagacggctg ggagcaacat attaccgccc gctatctggt cggtgccgat ggcgcaaact
    28621 cgatggtgcg gcgacatctc tacccggatc atcaaatccg taaatatgtc gctatccagc
    28681 agtggttcgc agagaaacat ccggtgccgt tctactcctg catctttgat aatgcgataa
    28741 ctgactgtta ctcatggagt atcagcaaag atggttattt tatctttggc ggtgcctatc
    28801 caatgaaaga cggtcagacg cgtttcacga cgctgaaaga gaaaatgagc gcctttcagt
    28861 tccagtttgg caaagcggtg aaaagtgaga aatgcacggt gttgtttccc tcacgctggc
    28921 aggattttgt ctgtggtaag gacaacgcct ttctgattgg tgaagcggcg ggatttatca
    28981 gcgccagctc gctggagggg attagctatg cgctggatag cgcagagatt ctgcgtgcgg
    29041 tgttcctgaa acagccggag aagagcaacg ccgcttactg gcgcgccaca cacaaactgc
    29101 gtttaaaact cttcggcaag atagtaaaaa gccgatgcct gaccgcaccg gctttaagaa
    29161 agtggattat gcgcagtggt gtggcgcata ttccacagtt gaaagattat ccaacgcgct
    29221 tcacatcacc caccagcagg atgtaagaga gcgcgccaat caacgcgacg gcggagatat
    29281 aaaccagtgc aggcgcgaaa ccgtaaccct gcgccaggta ccccaccacc agcggaacgg
    29341 tgatgccgcc cagaccaccg gcgaagttaa acacgccgcc ggttaaacca atcaggcgca
    29401 tcggtgccag agaagagacc agcgaccagg taatcgaagc aaaaccgttg ccgaagaatg
    29461 ccagcgccat caggcacata atcatcatcg gatcgttggt gtagttagcg cccataatgc
    29521 aggtggagat cagcaagccg cagataatcg gcgttttacg cgcaaagccc agtgaaaagc
    29581 ccttacgtac cagcagatcc gcgacccagc cagagagcag gacgccgaca aacgccgcga
    29641 ggaatggcac cgtggtcata aagcctgctt tcagcgccgt gattcctttt tcctgggtta
    29701 aatagttcgg gaaccaggtt aagaaaaacc acagtgcaga agccaccgca aattgcccaa
    29761 gataaacgcc gatcagttta cgatggaaca ccagtttcca gtctttggct gttaacggct
    29821 gacgcgcctc tttcttcacc ggcgcatcac catccaccag accgccgcca tcacgaatgt
    29881 aatccagttc agctttgctg atacctttgg tcaggcgcgg cggctgataa accttaaacc
    29941 aaatcagcga ccagataatg ccgataccac cagtgacaat gaacacccag tgccagctca
    30001 acatctcctg aatccagatc agcagcggag tcaaaaacgc cagaccgaca aactgaccag
    30061 acgtataaaa accaacggcg gaagcgcgtt catgttccgg gaaccagctg gtcaccatcc
    30121 ggttattggt cgggaaagcg ggcgcttcga aaataccggt tatcgcacgc agaccaatta
    30181 atgacattaa tcccgtggca aagccttgga ataaagtcgc cactgaccaa ccaaatatcg
    30241 caataaaata agtcacgcga gaacctacgc gatctaaaaa ccaaccgccg gggatctggc
    30301 atagcgtata aagccaggcg aaggccgaaa atacatagcc catttccgct ttcgtaatgc
    30361 cgaactcttc ctgaatatgg gcggaagcca cggccaggtt agcgcggtca acataacaaa
    30421 tgaccaccgt aataaagatc atcaccagcg tcagataacg ccgacgcccc ggctttgctg
    30481 cattaacggg aatatccata gcgagctttc tccagatttt gggcatagcg aagccgctca
    30541 ccatgccctg taatttacag agggttatat ttttgtattg ctgttttagt gcccgatgag
    30601 gggcttacgt ggcaggaatt accactctgc tacgctgtta tcttcatgac gccagagcgg
    30661 attacgccag tccggggcat ttttactgaa ctcaataact ttagcttcgt cgatttccac
    30721 acctaagccc ggtttcgtta acggtttaaa gaagccgcca accatgctga agtcttcttt
    30781 gtttttcaca aagtcgagta actccgcgcc tttgttgtaa tgaatgccca tactttgtta
    30841 ctgaagtacc gcgttatagg aaacgaagtc gatatgcagg caagccgcca gtgcaatcgg
    30901 tccgaacgga cagtgcggcg caagggtcac atcataggct tctgccattc cagcaatttt
    30961 gtagcattcg gtaataccgc ctgcatggga gagatccggt tgcagaatcg aaataccgcc
    31021 tgcctccagc acgcgtttaa aatcgaaacg tgagaacatg cgctcacccg ccgccagtgg
    31081 aatatgcgtt tgtgccgcca atttcgggta gtattcggct tgttccgcca gcaccggctc
    31141 ttcaataaac agcgggcgat acggctccag ttctttaatc agcactttcg ccattggcgc
    31201 gctgacgcga ccgtggaaat caagaccaaa ctcaatctga ttgccaaaag cttcacgaat
    31261 ttgtgccacg gtgttgactg ccgcatctac cgcgcgggag ttatcaatta gccccagttc
    31321 ttcacaaccg ttcagtttga aggtatcgaa gccgatttcg cgcagggttt taatgccgtc
    31381 gataacatcc gccggacgat cgccgccgac ccaactgtag gctttaattt tgtcgcgaac
    31441 caggccgccc atcagttgcc agactggcgc attcagcact ttgcctttga tatcccataa
    31501 cgcctggtcg atcccggcga tggcgctcat caggattgga ccgccacgat aaaatccggc
    31561 gcgatacatc acttgccata agtcgttgat gcgcgaagga tcctgaccaa tcaaatagtc
    31621 acccagctcg tgaactgccg cttccaccgt acgggcgcgg ccttcaatca cgggctcgcc
    31681 ccaaccgacc acgccttcat cggtttcaat tttcaggaac atccagcggg gaggtaaacg
    31741 atacgtggta attttggtga ttttcattgc actgcctctc gatacgcctt aacaaatgct
    31801 gctgcctgct gcgcggtgcg ttctacggac tgcccggcgc gataaagatc gctgcctaag
    31861 cccgccccaa cacaacctgc gtctatccac tgcgccaggt tttctggcgt cacgccgcca
    31921 acggcaaaga ctgcgatgtc cggtggtaat accgctttta acgctttgat gtattgcgga
    31981 ccaaaagccg atgacggaaa tattttcagc gcctgcgcgc ccgcttcgag cgcggtaaag
    32041 gcttcggtcg ccgtcgcgca gccggggcaa acggtcatgc cgtagcccac cgcgcggcgg
    32101 atcacttcac tatggatatt gggcgtaacg atgagctgac agcccatcct ggcgagcgca
    32161 tcgacctgtt caggtttcag taccgtacct gcgccaatca acgccttgtc gccatacgca
    32221 tcaacgatgg cgggaatgct ctgctcccat tgtggggaat tcagcgggat ttcaaccgcg
    32281 tcgaacccgg cgtcaatcac cgcgccaaca tgcgccagcg cctcgtcggg cgtaatgccg
    32341 cgcaaaatgg cgatcagcgg gagtttagtt tgccactgca tgagcgatgc tccttatacc
    32401 agcctgaaat gccgtgtcgc ccgccaccgc cgccacgtcg caacccatcg cctgaaaggc
    32461 ttgctggtag cgcgcggtca gcgatgttcc ggcgacaagg gtgatggcgt gttgatgggt
    32521 cacatagtcg cgcatactgg caacctctgc gccaatcaac aaaccagaga gaaattcgct
    32581 gacctgttcg cggggaagtg ttcccagcac atgcgaggcg cgaacttcaa aaagctgcgg
    32641 caatatggcg ggcgcattaa ggccgcgctc aaggccagcc gcgaaggcat cggcagagtt
    32701 ttcctgcggc ggcaaacctg cgccaatcaa tgagtgattt aacagtaaat gatgtaattc
    32761 accggtcatc acggtgcgaa aatcgttgat ttgctggcta tcggcctgca cccatttgca
    32821 atgcgtaccg ggcataacat aaagagagga aggagccaga gctcgcgcgc cgatcaattg
    32881 tgtttcttcg ccgcgcatca cattgtggtt atcgtcatga gagacgcata atccgggaat
    32941 aatccagata ttgtcgccaa cagacgttaa ttgttcgcca atagacgaaa aacgggcagg
    33001 aacagataaa tacggagcaa ctttccagcc gacattgctg ccgaccattc ctgccatcac
    33061 cactggcgta ttttcttcac gccagtcggt cgtgacttct gctaacactg cagccggaga
    33121 ttttccgttc aggcgcgtga cgcctgcttc tgattgcctg ctctccaggc agtggtcgcc
    33181 ctgataaagc caggcgcgca gattggtcga tccccagtca attgcgatgt agcgagctgt
    33241 catgtgattt cctttaacct tcgtgtcgag ctggcaatca tggtaagcgc cgcctgctct
    33301 gccgcatcgc cgtcctgatg ccgtatcgca tcgaacagcg ccttatgttc ctggagcgtt
    33361 tgcggcatgt tggcctcatc gcccatccag gttcgttcaa aaaccgcccg ctgcagcgaa
    33421 ctgatcgcaa tgctaagttg ctgtaacacc gggttatgca ccgactgcag caccgcctcg
    33481 tggtagcgaa tatccgcttc gttaaacgct tcgcggtcct gattgttggc aatcatctcg
    33541 ttcagcgccg attcaatctg cgccagatcg ctggaggtcg cgcgctctgc tgcccaacgg
    33601 gcaatcgccg gttccaccag atttcgcact tcgctcatgg cactgataag ccgtgggtcg
    33661 tagtcgtttt ccagcaccca ttgcagtacg tcagtgtcga ggtaattcca ctggttacgc
    33721 ggtgccacaa acgccccgcg ataacgtttc atttcaatca gccgcttcgc catcagcgaa
    33781 cggaacacct cacggatgat gttgcgcgag gttgcaaact cttcacagag ctccgcctca
    33841 gccgcaagtg gcgagccagg tacgtatttg ccgtgaacga tctgtttacc cagcgtaatg
    33901 acaatgcgat cggttttatt gagagtcatg gagagtcctt gtgctctgta tgttcttctc
    33961 tactttaccc cgatcgatgc ataacgcggc aactttgtag taccagcgtg atgacgttcg
    34021 cgtttgccgc gcgcgtaatg tagtacaaac ttatattgtt gcgctacaat ttagatcaaa
    34081 aaaagaacaa cacataaaaa aatgacatgc gtcgggcaga aatctgaaaa gggatatcag
    34141 gcgctaaaca ggagggaaag aagagtatgc tttcaacggc ttagctactc gtttaaagga
    34201 ttaatcatga agttgaattt taagggattt tttaaggctg ccggtttatt cccactggcg
    34261 ctgatgcttt caggctgtat ctcgtatgct ctggtttccc ataccgcaaa gggtagttca
    34321 ggaaagtatc aatcgcagtc agacaccatc actgggctat cgcaggcaaa agatagtaat
    34381 ggaacaaaag gctatgtttt tgtaggggaa tcgctggatt accttatcac tgatggtgcc
    34441 gatgacatcg ttaagatgct caatgatcca gcacttaacc ggcacaatat tcaggttgcc
    34501 gatgacgcaa gatttgtttt aaatgcgggg aaaaagaaat ttaccggcac aatatcgctt
    34561 tactactact ggaataacga agaagaaaag gcactggcaa cgcattatgg ttttgcctgt
    34621 ggtgttcaac actgtaccag gtcactggaa aacctaaaag gcacaatcca tgagaaaaat
    34681 aaaaacatgg attactcaaa ggtgatggcg ttctaccatc catttaaagt gcgattttat
    34741 gaatactatt cacccagagg cattccggat ggtgtttccg cagcattact gccagtgact
    34801 gttacactgg acatcattac tgcaccgctg caatttctgg ttgtatatgc agtaaaccaa
    34861 taatcagtaa gcgggcaaac gcgtttatgc tgtttgcccg cccacagatt aattcagcac
    34921 atacttctca atagcaaacg ccacgccatc ttcaaggttg gattttgtga caaagttcgc
    34981 cacttctttc accgaaggaa tagcgttatc catcgccaca ccgacgcctg catattcaat
    35041 cattgcgatg tcgttttcct gatcgccaat cgccatgatt tcttccggtt taatacctaa
    35101 cacgtcggcc agtgatttca cacccgtacc tttgttaacg cgtttatcga ggatttcgag
    35161 gaagtacggc gcacttttca gcacggtata tttctctttc acttcctgcg gaatacgcgc
    35221 gatagcctgg tcgaggatgg cgggttcatc aatcatcatc actttcagga actgggtatt
    35281 ggggtccatt ttctccgctt cgcagaacac cagcggaatt gtggcaacga aggattcatg
    35341 caccgtgtag tagctgatat cgcggttagc ggtgtacagc gtggtgcggt ccagggcgtg
    35401 gaaatgagaa ccgacttcgc gagagagttt ttccaggaaa cgatagtcgt catagctgag
    35461 agcggtttgc gccacggtgc taccatcagc ggccttctgt accagcgcgc cgttataagt
    35521 aatgcagtag tcgcccggct gttccatatg cagctctttc aggtagttgt gcacacctgc
    35581 atacggacga cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg cggcaatcgc
    35641 atttttaacg gcgggtgaaa tggtgtgatc gggcagcaga agggtgccat ccatatcgat
    35701 agcaatgagt ttgatagcca tgagttccca aggtagatta gttcctgacc catgctaacg
    35761 cgattccgct caaaaatcag tacaacaccc gagggaaaag gggatgcaac gcgggttgca
    35821 tgctccccat ttgcttagcg gaagagtttc cctttcagca gttccatgcc tgcggaaagc
    35881 agatcgttat tggcttgtgc tgacacttca ccttgcggtg agagcgcatc aataatcttc
    35941 ggcaattgct ctgccagtaa actggaagcc gtactggtat ccacgccaag tttttgcccg
    36001 agatcggaca ccgcatttgt gccgagcgcc gactccagtt gctcgccgct aactgattga
    36061 tttctctgtt gattactcaa ccaggttgag agaatggccc ctaagccgcc actttgcagt
    36121 ttttccagca gcacctgaat gccaccctgc tcctcaaccc aacttaaaat cgcctgatat
    36181 ttccccgcat cgcctttcag aaaggcaccg acaacttcat caaaaagccc catgataatc
    36241 acctgtaaag cgttacgtgt tgacccaaaa agtatagatc tgcgggatga taattgcgga
    36301 ttgcagaaat aaaaagggcg gagatgatct ccgccctttt cttatagctt cttgccggat
    36361 gcggcgtgaa cgccttaccc ggcctacaaa atcatgcaaa atcaatatat tgcaggagtc
    36421 tcgtaggact aataagcgta gcgcatcagg cacgctcgca tggttaacgc cattaaatat
    36481 caatattcgc cgctttcagg gcgttctctt caataaacgc acgacgcggt tctacggcgt
    36541 cgcccatcag cgtggtgaac aactggtcag cggcaatcgc atccttaacg gtaacgcgca
    36601 gcatacgacg gctttccggg tccatggtgg tttcccacag ctgttccggg ttcatctcgc
    36661 ccagaccttt ataacgctgg atggagaggc cacgacggga ctctttcacc agccagtcca
    36721 gcgcctgctc gaagctggct accggctgac gacgctcgcc acgttcgata aacgcatctt
    36781 cttccagcaa gccacgcagt ttctcaccca gcgtgcagat acgacgatat tcgccaccgg
    36841 tgataaactc gtgatccagc ggatagtcag tatccacacc gtgggtacgc acgcgaacaa
    36901 tcggctcgaa caggttttgt tcggcattgg tgtggacatc aaacttccac tggctgccgt
    36961 gctgttcttt gtcgttcagt tcgctgacca gcgcgttcac ccagcgggta acggtctgct
    37021 catcagaaag gtcagcttcc gtcaacgtcg gctgatagat aagctctttc agcattgctt
    37081 tcggataacg acgctccatg cggttgatca ttttctgcgt cgcgttgtac tcagatacca
    37141 gtttctctaa cgcttcgcca gccaatgccg gtgcactggc gttggtgtgc agcgttgcgc
    37201 cgtccagcgc gatagagatc tggtactgat ccatcgcttc gtcgtcttta atgtactgtt
    37261 cctgcttgcc tttcttcact ttgtacagcg gcggctgagc gatgtagacg tgaccgcgtt
    37321 caacgatttc cggcatctga cgatagaaga aggtcaacag cagcgtacga atgtgcgagc
    37381 cgtcgacgtc cgcatcggtc atgatgatga tgctgtgata acgcagtttg tccgggttgt
    37441 actcgtcacg accgatacca cagccaagcg cggtgataag cgtcgccact tcctgagaag
    37501 agagcatctt atcgaagcgc gctttctcga cgttaaggat tttacccttc agcggcagaa
    37561 tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc gcccgcggag tccccttcca
    37621 ccaggtacag ttcggaaagc gccggatcgc gttcctggca gtctgccagt ttgcccggca
    37681 ggccagctaa gtcgagcgca cctttacggc gggtcatttc acgcgcgcga cgcgccgctt
    37741 cacgggcacg ggcagcatcg ataattttgc caaccacgat tttcgcatca gttgggtttt
    37801 ccagcaggta ttccgccagc agttcgttca tctgctgttc aaccgccgat ttcacctcag
    37861 aagaaaccag tttgtctttg gtctgggagg agaatttcgg gtccggcact tttacggaaa
    37921 cgaccgcaat caggccttca cgcgcatcgt caccggtggc gctgactttg gcttttttgc
    37981 tgtagccttc tttgtccatg taggcattca gggtacgggt catcgccgca cggaagcctg
    38041 ccaggtgagt accgccgtca cgctgcggaa tgttgttggt aaagcagtag atgttttcct
    38101 ggaagccatc gttccactgc agcgccactt caacgccaat accgtctttt tcggtggaga
    38161 agtagaagat atttgggtgg atcggcgttt tgttcttgtt cagatattca acgaacgctt
    38221 tgatgccgcc ttcatagtgg aagtggtctt ctttgccgtc acgcttgtcg cgcagacgaa
    38281 tggaaacgcc ggagttgagg aacgacaact cacgcagacg tttcgccaga atttcatatt
    38341 cgaactcggt cacattggtg aaggtttcga ggctgggcca gaaacgcacc atggtgccgg
    38401 ttttttcagt ctcgccggta accgccagcg gagcctgcgg tacaccgtgt tcgtagatct
    38461 gacggtgaat tttaccctcg cgctgaataa ccagctccag tttttgcgac agggcgttta
    38521 ctaccgaaac accaacgccg tgcagaccgc cggacacttt ataggagtta tcgtcaaatt
    38581 taccgcctgc gtgcagaacg gtcatgatca cttccgccgc cgatacgccc tcttccgggt
    38641 gaataccggt cggaatgccg cgcccgtcat cctgtacaga gacagagtta tcggcgtgaa
    38701 tggtgacgat aatttcttta cagtgacccg cgagcgcttc gtcgatagcg ttatctacca
    38761 cctcgaatac catgtggtgc agaccggtgc cgtcatccgt gtcgccgata tacatacccg
    38821 ggcgcttacg caccgcatcc agccctttca ggactttgat actggaggag tcataagaat
    38881 tcgacatcaa cgtttctcgc tcatttatac ttgggttaat ccgttatttt acccttttcc
    38941 acggtaaaca tcttcgaatt ttcgtccgac atgtctataa cgtgttcagc actgatcgcg
    39001 ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg caagcaaccc gcgacgctca
    39061 tcatcaagct cagaggcaaa atcatctatc aggtagagac accgccgccc gctttcacga
    39121 gtgaggaact ctccttgcgc cagacgtaag gcgcacatca acagcttaag ctgcccacgc
    39181 gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc gtaagtccgc tttatgcggg
    39241 ccatgcgcgg tgtaggttag ctggcgatcg cgttcaaaat tacgttccag cacctcagcg
    39301 tactctgtct ctttctccca gccgcgctgg aaagagaaag tcagagaaaa ctcagggaga
    39361 aattgcttac aggtatcatc catatcggcc gcgataccgg cgctatactc cgcgcgccag
    39421 gtgctgattt gctccgccag cgggatcagt tctttatccc acgggcgtag ctgttcgtaa
    39481 cgagtcacct ggcgcagcgc cgcattgcgc tgcttgagca atcgcttgag attgctccag
    39541 gcggtgaaaa atccaggttc gttgtgaaag catccccagt cgaggaatgc tcttctgtat
    39601 ttggggccgc cgttgagtaa agtaaaccct tctggcgtta tcaactgcat cggcatcagg
    39661 tgcgccagtt ccgcgacttt atgcccgtcc gtaccgtcaa tacggacttt gctgtcgccc
    39721 tgtttgtctt tggttaagcc aatcgccgtc tcgcgctctt cgccctgtaa tcgcccgtgg
    39781 agaacaaatg cctcctgctc atggcgaatg acgcgaccaa tctgcaaact gcgaaacgcc
    39841 cgaccatggc cgagcgtata gatggcttcc agcacgctgg ttttgccact gccgttggca
    39901 cctaccagaa agttaaagcc gggagataaa gcgagatccg cggtttcaat attgcggaaa
    39961 tcgcggatca acaagcgggt gagggacatt acagtctcat tggcatgaca acataggcag
    40021 ccgactgtga tgcggcatct tcaatctgca cgctggaaac cgaatcggtc agcatcatgc
    40081 ggacgttttc gcatttcagc gcgttcagaa catccagcac atagctgacg ttgaagccga
    40141 tttccatctc cgcaccgcta taggtaacgt cgaggatctc ttccgcttct tcctgttccg
    40201 ggttgttggc agtaattttc agctggtttt cgctgacata caggcgcaca ccgcggaatt
    40261 tctcgttaga gagaatcgcc gcgcgagcaa acgcctgctt gagcagatcg cagcctgctt
    40321 ccagatgttt gtccgggttc ttcggcagaa cgcgacggta atccgggaag cgaccatcca
    40381 ccagtttcga ggtgaaaata aagtcgccaa cgtgggcacg aatgttattg ctgccaatct
    40441 gcacacgcag cagattgtcg ccgccgtcga gcatacgcat cagttcaatc acgcctttac
    40501 gcggtacgat caccgaatgg cttggcaaag attgaccaat tggcattgaa cagaccgcca
    40561 gacggtggcc gtcggttgcc acggtgcgca gttcttcacc ttcggtttca aacagcatac
    40621 cgtttaagta atagcgaaca tcctgatgcg ccatcgaaaa ctgggttgct tcaatcagac
    40681 gcttcatcgt cgcctgcggc agggtaaatt cgacttcgct ctgccagtca tcgaggttcg
    40741 ggaaatccgc cgctggcagg gtagacagcg aaaaacggct acgcccggag cgcaccagca
    40801 tccgttcacc ttccagctgc acggcaattt ccgcgccttc aggcagacca cggcagatat
    40861 caaagaattt gcgcgccgga acggtcgtcg ctcctggctc gtgtggctga accagcgcaa
    40921 cgcgtgccac catttccatc tcgagatcag taccggtcag cgacaacgta ccgtcagcaa
    40981 cctgtaacag cagattaccg agaatcggta gcgtaggacg accacctaac ggaccgctca
    41041 cctgttgtag cggttttaat aaatgctcac gttctacggt aaatttcata ggtttacgat
    41101 gacaatgttc tgattaaatt tgaaaaatct tctttgatat cgtggctctc ttcacgcaac
    41161 tgctcgatct tacggcaggc atgaagcacc gtcgtgtggt cacgcccacc aaacgcatcg
    41221 ccaatctctg gcagactgtg gttagtcagc tctttcgcta gcgccatcgc catctggcgc
    41281 ggacgcgcca ccgagcggga tcgacgcttg gaaaggagat ccgcgacttt gatcttgtag
    41341 tactccgcca ccgtcttctg aatattgtcg atggtgacca gtttttcctg caatgccagc
    41401 aagtcgcgca gcgcctcacg cacgaagtcg atggtgatcg cccgtccggt aaagttggcg
    41461 ttagcaataa cgcggttcag cgccccttcc agctcacgta cgttagatcg tagacgcttg
    41521 gcgataaaga acgccacttc acccggcaaa cgaatgtcat tttcgtcggc ctttttcatc
    41581 aggatcgcca cacgggtttc cagctctggc ggttcgatcg ccacagtcag tccccaaccg
    41641 aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt tcggatagcg atccgaggtg
    41701 agaatgatct gttgattacc ttccagcagg gcgttgaagg tgtggaaaaa ctcttcctga
    41761 gatcgttctt tattagcaaa aaactgaata tcgtcgatca gcagtgcatc tacggaacgg
    41821 tagtagcgtt taaactcttc gatcgcgttg ttttgcaggg ctttaaccat gtcctgaaca
    41881 aagcgctcgg agtgcatata aaccactttg gcattcggct tgcgcgccat aatgccgtta
    41941 cccaccgcat gcagcaggtg agttttaccc agacccgtgc cgccataaag gaacaacggg
    42001 ttataggcac cgccagggtt atccgccacc tggcaagccg ccgcgcgcgc cagttggtta
    42061 gatttacctt caacgaagtt atcaaacgtg tgtttgacgt ttacgttaga acgataggtc
    42121 ggttctgccg gggccgggac gttatcccaa cctgagcgcg tagaaggcgc agcacgttgc
    42181 ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc tcgtcactgc cgcttgtggc
    42241 gtttgcgtca ccggtttggt gccgacttca aaacgcagct gtggggcatc cgctccgcag
    42301 aaactggtta gcagtccatt gatattatta aggtacttgt cccgtaccca atcgaggaca
    42361 aaacggtttg gcgcgtacag ggccagcgtg ttatcgctca gttccgcctg caatgggcgt
    42421 atccacatac tgaattctgt ggctggtaac tcatcctgca atcgggcaag acactgctgc
    42481 caaagcgaaa gtgacacggc ggactccact cgaacaaaag tcgataatga ctaaggctga
    42541 aacattcatg attgttgacg tacgtcgaaa agaccctgct tgagggtgac gcacgaaccg
    42601 ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg atcgggaccg cggatcatag
    42661 cctaaactgc gcaagagatc ttctgtttct cacagatttt ccctatttat ccacaggact
    42721 ttccagaact cgctaagtgt aaacgatcct gccgcgaggc gggcacgatt tacgccgcat
    42781 attggaaaat ttaatgacca tagacaaaaa ttggcttaat cgatctaata aagatccagg
    42841 acgatccttg cgctttaccc atcagcccgt ataatcctcc acccggcgcg ccatgctggt
    42901 ttccactggt gtgaggtcgt acattttccc tgcgaaaagg tgcggaaaag cgcggtaaat
    42961 aaggaaagag aattgactcc ggtgtgtaca attattacaa tccggcctct ttaatcaccc
    43021 atggcttcgg tgtccatcgt ttcatttttc ggcggatatc caataaagcc attgaattta
    43081 ttcaagttta ggtagaaatc gccatgaaac gcacttttca accgtctgta ctgaagcgca
    43141 accgttctca cggcttccgt gctcgtatgg ctactaaaaa tggtcgtcag gttctggcac
    43201 gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa gtaataaagc taacccctga
    43261 gtggttaagc tcgcatttcc cagggagtta cgcttgttaa ctcccagtca attcacattc
    43321 gtcttccagc agccacaacg ggctggcacg ccgcaaatta ccattctcgg ccgcctgaat
    43381 tcgctggggc atccccgtat cggtcttaca gtcgccaaga aaaacgttcg acgcgcccat
    43441 gaacgcaatc ggattaaacg cctgacgcgt gaaagcttcc gtctgcgcca acatgaactc
    43501 ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg ccgacctcga taaccgtgct
    43561 ctctcggaag cgttggaaaa attatggcgc cgccactgtc gcctggctcg cgggtcctga
    43621 tagccctcat tcgggtctat caacgcctga ttagtccgct actcgggccg cattgtcgtt
    43681 tcactccaac ctgttcaagc tacggaattg aggcattgcg caggtttgga gtgataaaag
    43741 gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc tttacaccct ggtggtgacg
    43801 atcccgtccc gcccggacca tttgatacca gagaacacta acgatggatt cgcaacgcaa
    43861 tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc tggcaagcct gggagcagga
    43921 taaaaacccg caacctcagg cccaacagac cacgcagaca acgaccaccg cagcgggtag
    43981 cgccgccgac cagggcgtac cggccagtgg ccaggggaaa ctgatctcgg ttaagaccga
    44041 cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt gagcaagctc tgctgcctgc
    44101 ttacccgaaa gagctgaact ctacccagcc gttccagctg ttggaaactt caccgcagtt
    44161 tatttatcag gcacagagcg gtctgaccgg tcgtgatggc ccggataacc cggctaacgg
    44221 cccgcgtccg ctgtataacg ttgaaaaaga cgcttatgtg ctggctgaag gtcaaaacga
    44281 actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac acgtttacca aaacgtttgt
    44341 cctgaaacgt ggtgattacg ctgtcaacgt caactacaac gtgcagaacg ctggcgagaa
    44401 accgctggaa atctcctcgt ttggtcagtt gaagcaatcc atcactctgc caccgcatct
    44461 cgataccgga agcagcaact tcgcactgca caccttccgt ggcgcggcgt actccacgcc
    44521 tgacgagaag tatgagaaat acaagttcga taccattgcc gataacgaaa acctgaacat
    44581 ctcttcgaaa ggtggttggg tggcgatgct gcaacagtat ttcgcgacgg cgtggatccc
    44641 gcataacgac ggtaccaaca acttctatac cgctaatctg ggtaacggca tcgtcgctat
    44701 cggctataaa tctcagccgg tactggttca gcctggtcag actggcgcga tgaacagcac
    44761 cctgtgggtt ggcccggaaa tccaggacaa aatggcagct gttgctccgc acctggatct
    44821 gaccgttgat tacggttggt tgtggttcat ctctcagccg ctgttcaaac tgctgaaatg
    44881 gatccatagc tttgtgggta actggggctt ctccattatc atcatcacct ttatcgttcg
    44941 tggcatcatg tacccgctga ccaaagcgca gtacacctcc atggcgaaga tgcgtatgct
    45001 gcagccgaag attcaggcaa tgcgtgagcg tctgggcgat gacaaacagc gtatcagtca
    45061 ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg ctgggcggct gcttcccgct
    45121 gctgatccag atgccaatct tcctggcgtt gtactacatg ctgatgggtt ccgttgaact
    45181 gcgtcaggca ccgtttgcac tgtggatcca cgacctgtcg gcacaggacc cgtactacat
    45241 cctgccgatc ctgatgggcg taacgatgtt cttcattcag aagatgtcgc cgaccactgt
    45301 gaccgacccg atgcagcaga agatcatgac ctttatgccg gtcatcttca ccgtgttctt
    45361 cctgtggttc ccgtcaggtc tggtactgta ctatatcgtc agcaacctgg taaccattat
    45421 tcagcagcag ctgatttacc gtggtctgga aaaacgtggc ctgcatagcc gcgagaagaa
    45481 aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg tcagttgacc gccttttttc
    45541 ttttcgtagg gcggataagc accgcgcatc cgccacacaa agcaacagga acatcatgag
    45601 cgataatgac actatcgtag cccaggccac gcctccggga cgtggcggcg ttggcatcct
    45661 gcgcatctcc ggcttcaaag cccgtgaagt tgccgaaacc gtgctgggta aactgcctaa
    45721 gccgcgctac gccgattatc ttccgtttaa agacgccgac ggcagcgtgc tcgatcaggg
    45781 gattgcgcta tggttccctg gcccgaactc gttcaccggc gaagatgtgc tggaactgca
    45841 aggtcatggc ggtccggtga tcctcgacct gctgttaaaa cgcattctga ccattcccgg
    45901 cctgcggatt gctcgccctg gtgagttttc cgaacgcgcg tttcttaacg ataaacttga
    45961 cttagcccag gccgaggcga ttgccgatct tatcgacgcc agttcggaa
//
LOCUS       DAJTYM010000022        44759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTYM010000022 DAJTYM010000000
VERSION     DAJTYM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..201
                     /locus_tag="OO575_001244"
                     /pseudo
     CDS             1..201
                     /locus_tag="OO575_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            400..2088
                     /locus_tag="OO575_001245"
     CDS             400..2088
                     /locus_tag="OO575_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6664280.1"
                     /translation="MQSPLPYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNL
                     SDSKFISSFKSWFESGKIKNIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGE
                     FITYYENGAIESKRHFNNDKIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGK
                     LMQENFYQQSVLIGNDTSYYQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKD
                     GKQISVQTWYENGQKEEEKHFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEW
                     YENGIPESLYPYKNGKTDGIAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKE
                     TLYVNGIRQGVEKEFNDRTGKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLS
                     SLDNPTQIKDNATTGDSSAQFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATA
                     YKGNGIPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAA
                     QGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2141..3871
                     /locus_tag="OO575_001246"
     CDS             2141..3871
                     /locus_tag="OO575_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664281.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(3931..4422)
                     /gene="yajQ"
                     /locus_tag="OO575_001247"
     CDS             complement(3931..4422)
                     /gene="yajQ"
                     /locus_tag="OO575_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6664282.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4590..5501
                     /gene="panE"
                     /locus_tag="OO575_001248"
     CDS             4590..5501
                     /gene="panE"
                     /locus_tag="OO575_001248"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6664283.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5464..6054
                     /gene="yajL"
                     /locus_tag="OO575_001249"
     CDS             5464..6054
                     /gene="yajL"
                     /locus_tag="OO575_001249"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6664284.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6108..7556)
                     /gene="thiI"
                     /locus_tag="OO575_001250"
     CDS             complement(6108..7556)
                     /gene="thiI"
                     /locus_tag="OO575_001250"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6664285.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7762..8004
                     /gene="xseB"
                     /locus_tag="OO575_001251"
     CDS             7762..8004
                     /gene="xseB"
                     /locus_tag="OO575_001251"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6664286.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8004..8903
                     /gene="ispA"
                     /locus_tag="OO575_001252"
     CDS             8004..8903
                     /gene="ispA"
                     /locus_tag="OO575_001252"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6664287.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            8928..10790
                     /gene="dxs"
                     /locus_tag="OO575_001253"
     CDS             8928..10790
                     /gene="dxs"
                     /locus_tag="OO575_001253"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6664288.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            10845..11762
                     /gene="yajO"
                     /locus_tag="OO575_001254"
     CDS             10845..11762
                     /gene="yajO"
                     /locus_tag="OO575_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6664289.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(11873..12391)
                     /gene="pgpA"
                     /locus_tag="OO575_001255"
     CDS             complement(11873..12391)
                     /gene="pgpA"
                     /locus_tag="OO575_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6664290.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12369..13346)
                     /gene="thiL"
                     /locus_tag="OO575_001256"
     CDS             complement(12369..13346)
                     /gene="thiL"
                     /locus_tag="OO575_001256"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6664291.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13424..13843)
                     /gene="nusB"
                     /locus_tag="OO575_001257"
     CDS             complement(13424..13843)
                     /gene="nusB"
                     /locus_tag="OO575_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6664292.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(13863..14333)
                     /gene="ribE"
                     /locus_tag="OO575_001258"
     CDS             complement(13863..14333)
                     /gene="ribE"
                     /locus_tag="OO575_001258"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6664293.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14422..15525)
                     /gene="ribD"
                     /locus_tag="OO575_001259"
     CDS             complement(14422..15525)
                     /gene="ribD"
                     /locus_tag="OO575_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6664294.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15529..15978)
                     /gene="nrdR"
                     /locus_tag="OO575_001260"
     CDS             complement(15529..15978)
                     /gene="nrdR"
                     /locus_tag="OO575_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6664295.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16129..16668
                     /locus_tag="OO575_001261"
     CDS             16129..16668
                     /locus_tag="OO575_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6664296.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            16966..17850
                     /gene="tsx"
                     /locus_tag="OO575_001262"
     CDS             16966..17850
                     /gene="tsx"
                     /locus_tag="OO575_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6664297.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18027..18374)
                     /locus_tag="OO575_001263"
     CDS             complement(18027..18374)
                     /locus_tag="OO575_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6664298.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18503..19474)
                     /gene="secF"
                     /locus_tag="OO575_001264"
     CDS             complement(18503..19474)
                     /gene="secF"
                     /locus_tag="OO575_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6664299.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19485..21332)
                     /gene="secD"
                     /locus_tag="OO575_001265"
     CDS             complement(19485..21332)
                     /gene="secD"
                     /locus_tag="OO575_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6664300.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21360..21692)
                     /gene="yajC"
                     /locus_tag="OO575_001266"
     CDS             complement(21360..21692)
                     /gene="yajC"
                     /locus_tag="OO575_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6664301.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21715..22842)
                     /gene="tgt"
                     /locus_tag="OO575_001267"
     CDS             complement(21715..22842)
                     /gene="tgt"
                     /locus_tag="OO575_001267"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6664302.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(22897..23967)
                     /gene="queA"
                     /locus_tag="OO575_001268"
     CDS             complement(22897..23967)
                     /gene="queA"
                     /locus_tag="OO575_001268"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6664303.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24060..24633
                     /gene="acpH"
                     /locus_tag="OO575_001269"
                     /pseudo
     CDS             24060..24633
                     /gene="acpH"
                     /locus_tag="OO575_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24638..26455)
                     /gene="malZ"
                     /locus_tag="OO575_001270"
     CDS             complement(24638..26455)
                     /gene="malZ"
                     /locus_tag="OO575_001270"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6664304.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26611..27984)
                     /gene="proY"
                     /locus_tag="OO575_001271"
     CDS             complement(26611..27984)
                     /gene="proY"
                     /locus_tag="OO575_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6664305.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28060..29379)
                     /gene="brnQ"
                     /locus_tag="OO575_001272"
     CDS             complement(28060..29379)
                     /gene="brnQ"
                     /locus_tag="OO575_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6664306.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29786..31081)
                     /gene="phoR"
                     /locus_tag="OO575_001273"
     CDS             complement(29786..31081)
                     /gene="phoR"
                     /locus_tag="OO575_001273"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6664307.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31139..31828)
                     /gene="phoB"
                     /locus_tag="OO575_001274"
     CDS             complement(31139..31828)
                     /gene="phoB"
                     /locus_tag="OO575_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6664308.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32018..33220
                     /gene="sbcD"
                     /locus_tag="OO575_001275"
     CDS             32018..33220
                     /gene="sbcD"
                     /locus_tag="OO575_001275"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6664309.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33217..36360
                     /gene="sbcC"
                     /locus_tag="OO575_001276"
     CDS             33217..36360
                     /gene="sbcC"
                     /locus_tag="OO575_001276"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6664310.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36486..37667
                     /gene="araJ"
                     /locus_tag="OO575_001277"
     CDS             36486..37667
                     /gene="araJ"
                     /locus_tag="OO575_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6664311.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37810..38718)
                     /gene="mak"
                     /locus_tag="OO575_001278"
     CDS             complement(37810..38718)
                     /gene="mak"
                     /locus_tag="OO575_001278"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6664312.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            38843..39754
                     /gene="rdgC"
                     /locus_tag="OO575_001279"
     CDS             38843..39754
                     /gene="rdgC"
                     /locus_tag="OO575_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6664313.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39832..40287)
                     /locus_tag="OO575_001280"
     CDS             complement(39832..40287)
                     /locus_tag="OO575_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664314.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40284..40619)
                     /locus_tag="OO575_001281"
                     /pseudo
     CDS             complement(40284..40619)
                     /locus_tag="OO575_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40774..41058)
                     /gene="ppnP"
                     /locus_tag="OO575_001282"
     CDS             complement(40774..41058)
                     /gene="ppnP"
                     /locus_tag="OO575_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6664315.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41130..41807)
                     /locus_tag="OO575_001283"
     CDS             complement(41130..41807)
                     /locus_tag="OO575_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6664316.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42065..42256)
                     /gene="yaiA"
                     /locus_tag="OO575_001284"
     CDS             complement(42065..42256)
                     /gene="yaiA"
                     /locus_tag="OO575_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6664317.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42306..42830)
                     /gene="aroL"
                     /locus_tag="OO575_001285"
     CDS             complement(42306..42830)
                     /gene="aroL"
                     /locus_tag="OO575_001285"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6664318.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43013..43471)
                     /locus_tag="OO575_001286"
     CDS             complement(43013..43471)
                     /locus_tag="OO575_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6664319.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43591..44400
                     /gene="proC"
                     /locus_tag="OO575_001287"
     CDS             43591..44400
                     /gene="proC"
                     /locus_tag="OO575_001287"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6664320.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44417..>44759)
                     /locus_tag="OO575_001288"
     CDS             complement(44417..>44759)
                     /locus_tag="OO575_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6664321.1"
                     /translation="WGHDVGDEAIVALTRQLQITLRGSDVIGRFGGDEFAVIMSGTPA
                     ESAITAMLRVREGLNTLRLPNTPQVTLRISVGVAPLNPQMSHYREWLKSADLALYKAK
                     KAGRNRTEVAA"
BASE COUNT        11610 a        11677 c        10969 g        10503 t
ORIGIN      
        1 tcccctcccg caagcactat aactttcatt gcacaaatcg atcatgttga gctaaataaa
       61 acagcattat ctcatgatat gaaactcgtt cttgccgatc gtttttattt caaaaccagt
      121 aatccttgcg aaacaaattc aatttctgct cgaccagaat caatggatat tactgacaaa
      181 gcctatgccg acaaattaaa aaaattagaa actccttttc ttgatgatcg ctatttcttt
      241 atgaccatca atcagtgtga cggtaatggc actcccatgc tgacagacat aaaagtatgt
      301 acaacagaat tatgtggtgc tgaatataca aagaaaatgt ctactgtatg gctcgatgaa
      361 actttaaaac caactgctaa acgtctggca atttatttta ttcaatctcc tttaccctat
      421 gacgaggaga aaaaacaatg gaaagtcacc ggatggtata tctcatcaac tgataatcag
      481 tcttcaacta aacagattgc atttgaagga tatacagaca cagataacct ctccgattct
      541 aaatttattt cttcatttaa atcctggttt gaaagtggaa agataaaaaa cattgctaac
      601 tataatccaa aaggagaaag agatggaaaa tacgaaagtt attacaatag caatggtaac
      661 gtctatgaat ctctaagttt ttctaatggt cttatcaacg gtgagtttat tacatattat
      721 gaaaatgggg ccattgaaag taaacgtcat tttaataacg acaaaattaa agatggtgaa
      781 tgccctcatt tttatgaaga tgggaaaatc aaacagcaac atagctatct gaatagtaaa
      841 ctcgatggtc ctgcatatga atattatcct gatggcaaac tcatgcagga aaatttctat
      901 cagcaaagtg tgttgattgg aaacgatacc agttactacc aatccggaaa aataagctct
      961 atacacaata gaaattcccg tggtcagtat gatggagcta acgaacgtta tagtgaagag
     1021 ggtaagttac tgtctaaatc cgtctataaa gacggaaaac aaatttctgt tcaaacctgg
     1081 tatgaaaatg ggcagaaaga agaggagaaa cattttgatg agcaaggcca attaaatggt
     1141 ctggttaagc aatggtataa aaatggtaat cttgcaaaat cacaaaacta taaacatgac
     1201 atactcgatg gtaacagtga agagtggtac gaaaatggca tccctgaaag tttgtaccct
     1261 tataagaatg gaaaaaccga cggcattgcc aaatcatgga ataagtatgg taaattaacc
     1321 tattcaatcg aatacaaaaa tggcgtagag aatggggcat atcgtaactg gagcaaaaac
     1381 acaggtaaac taaccaaaga aaccctgtac gtcaatggta tcagacaggg agtggaaaaa
     1441 gagtttaacg atcgcacagg taaactatta acctccacgc aatatgttaa taataaaaga
     1501 catggtacgg aggaaactta tgatcaaaac ggaataaaat atattacatg ctatcaaaat
     1561 gatcagaaat tatcgtcttt agacaatcca acacaaataa aagataatgc aacaacaggc
     1621 gattccagtg ctcaatttgc attagggaaa tacgaattca tctgtgcaaa tatagatgaa
     1681 gggattaaat ggttaaccaa atctgcagag caaaaaaaca ctgatgcgat atacttttta
     1741 gctacagcct acaaaggcaa tggtatacct gcaaacaatg aaaaatacct agcatatctg
     1801 caacaagctg caacactggg cagtagtaat gcccaggcgg aaattggcta tttatatctc
     1861 atcggaaaag aactaccaca gaatcttcct gatgctggtg tctggttcaa aaaagcagct
     1921 gcacaaggta attttgccgc acatttctat ctgggaagga tgtatcagaa tggtgacggc
     1981 gttgagagaa atatggaaaa agccaggttt catctatcta atgcggcaga aggtggaata
     2041 gaacctgcgc ttaaagcatt aaatgaactt gaacatcaaa caaaataaag cgctcaggga
     2101 ccacgtaagg ttcatcaaac agaaaaggat atagtttgcg atgaaagcga aatccacttt
     2161 gttattaatg attttatctg ttttttattc tcatcaggtt ttatccagcg aacccactca
     2221 gccggatgtt tattctgtcg tcgagaaaaa actaaactct gctttacctc tggaagaaaa
     2281 taccaccttc agatcgcagg ctgaatggtt taatttgcaa tatgaattat tagtatctgg
     2341 ctaccctgaa cgtgcttaca atttactgag caaacttgag acagaatcga aaaacgctaa
     2401 atattatctg gatttaactg cctccagagt cgctgaagaa gaagcaccag agacagcctt
     2461 aactttttta tcaaaaattg ggttaaataa accggctact cttacatcct ttgagagcta
     2521 tatcaatagc tggataaaaa gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga
     2581 tgagaatgcc ctgaattatt atttaccgac tgtgttagtc gcatatcaga tcgttcctga
     2641 taaggcaata agtatttata attctgtata tggagataat gttgttatac cctatcagca
     2701 agtcaccatg ttacttacag ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc
     2761 cattcattat gccgataagg cgctgaatat gtttgataaa gctattgctg tagatagcag
     2821 tcgtgaatcc cattatagtg atgagtattt gaaactcatg aatatctacg ccgccaatgg
     2881 caacaaagaa aaagcggtgg ttttatctca acgtctacac aaagccatta ccgaatctca
     2941 atcgaattac ttaagtactc tggaaggaat tttattgttt tatagaaata atgatatgca
     3001 gcaagactat caaaacagcc tttcaaatta catagtattt atcgataaaa tttttgcctt
     3061 ttccccatca acaagaagtg aattaagcct cattaattta ttaaattcat taaatgaggt
     3121 ggaattgatg agaaaacgtc tggcgtttct gatgacagca ccagaatacg cttgttataa
     3181 tagtcagtat tgttatgaag acaaagtcag cgcattaaaa atcctgtatc gtcatcatga
     3241 caatgagcta atggacaaat atttcacgac tcttcttaaa gagatcagta atcaaccatt
     3301 agaagactgg gatataataa taagtaatac tggtgaaaaa tttagtgaag tgggtctaac
     3361 caaatatgct caacatctcg catctttcgc tgaagaattt tatataaaag agcaaaaatc
     3421 acattctgaa agtgaaatga gaggactatt ttcatcactt gccgaattat atagttttgg
     3481 taatgatact gccagcgcaa accgagtgtt ccatcagcat gtccctgcct tcaatacaga
     3541 tcatatgatt caatatttta taaatgccaa agaatggaac aatgccaggg aattattgat
     3601 aaaagaggaa atgttgggta tgcataatct gatattgttg gagaatattt gtatgcaaaa
     3661 aaatgcagaa tgcatggcac acattacctt tacgcttaat aaattaacaa cacaaccagc
     3721 aataacaaaa atagatagcg tgggtaatga gcaactttat cagattggca aaatctacca
     3781 taagttggaa attaaacctg agccagagca acaggtttta attcagtcgc tttatgaccg
     3841 ggcatcagga agcgcctctg caactcaata aatgcatggt caaaagggcc accacggtgg
     3901 ccctgacaat cagtagctat atccagaaag ttaatcgcgg aagtttttga actggaacgg
     3961 ctgaccgaga tcgccaccgc gcaccatcgc catgacagcc tgcaaatcat cacgagattt
     4021 gccagtcacg cggatctcat cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt
     4081 gatcatcttg acgatttttt tctgagtggc gctctcaatg ccctgtttca gtttcgcttc
     4141 cacaaaccag gttttaccgc tatgaacgat attttccggt acatccagtg aactgccttc
     4201 aatgccgcgc ttcagcagct tggcacgcag aatatccagc aactgattga cctggaagtc
     4261 ggactcgctc aacactttga tggttttgct ggcgtcgttc agctcaaatg aggcttcaac
     4321 gttacggaag tcaaaacggg actccacttc gcgactcgcg ttatcgaccg cgttacgtgc
     4381 ttcctgaaga tcaacttcag agacaatatc gaaagatggc atcttttcct ctcccttcat
     4441 ttttgatgcg aagcataata cctgcaaagt taaataacca acagtcctgc accaggacgt
     4501 atcccttaat tgtgcatagt cgcaacagca tgcacaatta agggatagcc ttattgaaca
     4561 cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac cgtattggga tgcggtgcct
     4621 tagggcaatt atggcttaca gcactttgta aacagggtca tgaagttcag ggctggctgc
     4681 gtgtaccgca accttattgt agtgtgaatc tggttgagac agatggttcg atatttaacg
     4741 aatcgctgac cgccaacgat cccgattttc tcgccaccag cgatctgctc ctggtgacgc
     4801 tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc atccacactg cctgtaacta
     4861 cgccaatact gttaattcac aacggcatgg gcaccatcga agaattgcaa aacattcagc
     4921 agccattact gatgggcacc accacccatg ccgcccgccg cgacggcaat gtcattattc
     4981 atgtggcaaa cggaatcacg catatcggtc cggcacggca acaggatggc gattacagtt
     5041 atctggcgga tattttgcaa accgtgttgc ctgacgttgc ctggcataac aatattcgcg
     5101 ccgagctgtg gcgcaagctg gccgtcaact gcgtgattaa tccactgact gccatctgga
     5161 attgcccgaa cggtgaatta cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag
     5221 tcgcggcagt gatcgaacgc gaagggcatc atacttcagc ggaagatttg cgtgattacg
     5281 tgatgcaggt gattgatgcc acagcagaaa atatctcgtc gatgttgcag gatatccgcg
     5341 cgctgcgcca caccgaaatc gactatatca atggttttct cttacgccgc gcccgcgcgc
     5401 atgggattgc cgtaccggaa aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg
     5461 aatatgagcg catcggcact ggtttgcctc gcccctggta gtgaagagac tgaagccgtc
     5521 accacgatca acctcttggt tcgcggcggt atcaaagtca ccactgccag cgtcgacagc
     5581 gatggcaacc tggcaattac ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg
     5641 gtcgaagtgg ctgatggcga atatgacgtg atcgtgctgc ctggtggcat taaaggcacg
     5701 gagtgttttc gcgatagcac tctgctggtt gaaaccgtta aacagttcca ccgttccggg
     5761 cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt
     5821 ccgattggca atatgaccgg cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg
     5881 caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt
     5941 acagctatcg actttggtct gaaaattatc gacctgctgg ttgggcgcga gaaagcccat
     6001 gaagtggcat cacaactggt gatggcggca gggatttata attattacga gtagtgtcgg
     6061 atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat
     6121 accttcacat tgttaaagcc ttgctcgcgc agatagagcg cctgcagacg gctcatcacc
     6181 ccgcgctcac accacagcag ccaggttttg ttctggtcga gatcgccaaa tttggtgctc
     6241 agtttataga acggcagaga aaccacatca atcccttcga ctttcagtgg cttatcttcc
     6301 tgttcatcga cagaacggat atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt
     6361 tccacttcca ccacttcctg ctcggtctgc tgggcgattt cgcggatatc aacgttattc
     6421 gcttcctcaa ccactttatc gagaatactg aagtcgaact tctcttcttc cgcttcaatc
     6481 ttcgatttaa ccgctttcac cgtcgggctt ttggagatca cgccacaata ttccggcatc
     6541 gtgcgagcaa agtcttcggt gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg
     6601 taagaaatca gcggacgcag gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg
     6661 gtcagcgtct gactggacac ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg
     6721 taacgttcag ccactttaga tgcggcacgc accatcatac gtttgaggat aacgcccatc
     6781 tgaccgtcgt cgattttctc gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca
     6841 aaacgcacgc ggtgggagct gccaaagcgg ttccacagat aatgcgccac ctgacgcacg
     6901 ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt aatgcacgcg gcagccgcga
     6961 cgcatcaaca tataactgga aacaccggag tcgaaaccac cggaaatgag cgacagcaca
     7021 tcttcctggg tgccaatcgg gaaaccacca ataccttcgt agcgaccttt aatcagcagg
     7081 aggcgatcgt cttccacttc cagatgaaca gttacatccg ggttagtcag cttcacgcgc
     7141 gcggattcaa tatgctgatt taaaccgccg ccgacgtaac gttccacatc aatcgagcta
     7201 aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg ttttgccttc cagctgatcg
     7261 cgatactgaa ccaacgcttt ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg
     7321 acttcgagaa tatggtggat acccggaata cgggtcaggg cgtcgcgaat agtcagacgc
     7381 tggttttcat ctttcgcgcg aacttcgatg ttatcccagt ggcggacgac agcgagcgtc
     7441 tcatcatagt gctttaaaac gttacgaatg ttcccggtaa ggatttttat aaagcgcaag
     7501 cgcacagatt ggcttttgat ggtgatttcc gggaacaatt taatgataaa cttcatggcg
     7561 gcaatggttc gttggcaagc cttaagcgac ttgtataggg aaaaatacag cagcccacac
     7621 ctgcggctgc atccaggcgc ggaagtatac cactaacatc gctttgctgc gcacatcacc
     7681 ttaccattgc gcgttatttg ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat
     7741 attgagagtc agacattcat tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag
     7801 gcgctgagcg agctggaaca gattgtaacc cgtctggaaa gtggcgacct gccgctggaa
     7861 gaggcgctga acgagttcga acgcggcgtg cagctggcac gtcaggggca ggccaaatta
     7921 caacaagccg aacagcgcgt acaaattctg ctgtctgaca atgaagacgc ctctctaacc
     7981 ccttttacac cggacaatga gtaatggact ttccgcagca actcgaagcc tgcgttaagc
     8041 aggccaacca ggcgctgagc cgttttatcg ccccactgcc ctttcagaac actcccgtgg
     8101 tcgaaaccat gcagtatggc gcattattag gtggtaagcg cctgcgacct ttcctggttt
     8161 atgccaccgg tcatatgttt ggcgttagca caaacacgct ggacgcaccc gctgctgccg
     8221 tagagtgtat ccacgcttac tcattaattc atgatgattt accggcgatg gatgatgacg
     8281 atctgcgtcg cggtttgccg acctgccatg tgaagtttgg cgaagcaaac gcgattctcg
     8341 ctggcgacgc tttacaaacg ctggcgttct cgattctaag cgatgccaat atgccggaag
     8401 tgtcggatcg cgacagaatt tcgatgattt ctgaactggc gagcgccagc ggtattgccg
     8461 gaatgtgcgg tggtcaggca ttagatttag acgcggaagg caaacacgta cctctggacg
     8521 cgcttgagcg tattcatcgt cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg
     8581 gtgcattaag cgccggagat aaagggcgtc gtgctctgcc agtactcgac aagtacgcag
     8641 agagcatcgg ccttgccttc caggttcagg atgacatcct ggatgtggta ggagatactg
     8701 caacgttggg aaaacgccag ggtgccgacc agcaacttgg taaaagtacc taccctgcac
     8761 ttctgggtct tgagcaagcc cggaagaaag cccgggatct gatcgacgat gcccgtcagg
     8821 cgctgaaaca actggctgaa cagtcactcg atacctcggc actggaagcg ctagcggact
     8881 acatcatcca gcgtaataaa taaacaataa gtattaatag gcccctgatg agttttgata
     8941 ttgccaaata cccgaccctg gcactggtcg actccaccca ggagttacga ctgttgccga
     9001 aagagagttt accgaaactc tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc
     9061 gttccagcgg gcacttcgcc tccgggctgg gcacggtcga actgaccgtg gcgctgcact
     9121 atgtctacaa caccccattt gaccaattga tttgggatgt gggacatcag gcttatccgc
     9181 ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc
     9241 acccgttccc gtggcgcggc gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa
     9301 cctccatcag tgccggaatt ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc
     9361 gcaccgtctg tgtgattggc gacggcgcga ttaccgctgg catggcgttt gaagcgatga
     9421 atcacgcggg cgatattcgt cctgatatgc tggtggtcct caacgacaat gaaatgtcga
     9481 tttccgaaaa tgttggcgcg cttaacaacc atctggctca gctgctttcc ggtaagcttt
     9541 actcttcact gcgcgaaggc gggaaaaaag ttttctctgg cgtgccgccc attaaagagc
     9601 tgctcaaacg taccgaagaa catattaaag gcatggtggt gcctggcacg ttgtttgaag
     9661 agctgggatt taactacatc ggcccggtgg acggtcacga tgtgctgggg cttatcacca
     9721 cgctaaagaa catgcgcgac ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag
     9781 gtcgtggtta tgaaccggca gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg
     9841 atccctccag cggttgtttg ccgaaaagta gcggcggttt gccgagctat tcaaaaatct
     9901 ttggcgactg gttgtgcgaa acggcagcga aagacaacaa gctgatggcg attactccgg
     9961 cgatgcgtga aggttccggc atggtcgagt tttcacgtaa attcccggat cgttacttcg
    10021 acgtggctat tgccgagcaa cacgcagtga cctttgccgc cggtctggcg attggtggat
    10081 acaaacccat tgtcgcgatc tactccacct tcctgcaacg cgcctatgat caggtgctgc
    10141 atgacgtggc aattcaaaag ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg
    10201 gtgctgacgg tcaaactcac cagggcgctt ttgatctctc ttacctgcgc tgcataccag
    10261 aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg ccagatgctc tataccggct
    10321 atcactataa cgatggcccg tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg
    10381 aactgacgcc gctggaaaaa ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga
    10441 aactggtgat ccttaacttt ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc
    10501 tgaacgctac gctggtcgat atgcgttttg tgaaaccact tgatgaagcg ttaattctgg
    10561 aaatggccgc cagccatgaa gcgctggtca ccgtagaaga aaacgccatt atgggcggcg
    10621 caggtagcgg agtgaacgaa gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca
    10681 ttggcctgcc ggacttcttt attccgcaag gaactcagga agaaatgcgc gccgaactcg
    10741 gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg gctggcataa tccctactcc
    10801 actcctgcta tgcttaagaa attattcatg gcaggagtgg cagcatgcaa tacaacccct
    10861 taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc
    10921 cagatcgcgg taatcacgca tggacactgc cggaagagag cagccgtccc ataattaaac
    10981 gcgcgctgga aggtggcata aatttctttg ataccgccaa tagctattcc gatggtagca
    11041 gcgaagagat cgttggtcgc gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg
    11101 ccaccaaagt gttccatcgc gttggtgatt taccggaagg attatcccgt gcacaaattt
    11161 tgcgctctat caacgacagc ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa
    11221 ttcatcgctg ggattacaac acgccgatcg aagagacgct ggaagccctg aacgacgtgg
    11281 taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat gcacgcttcg cagtttgctc
    11341 aggcgctgga actacaaaaa cagcactgct gggcgcagtt tgtcagtatg caggatcact
    11401 ataatctgat ttatcgcgaa gaagagcgcg agatgctgcc tctgtgttat caggagggcg
    11461 tggcggtgat tccgtggagc ccgctggcgc gggggcgact gacgcgtcca tggggagaaa
    11521 ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa
    11581 atgacgcgca gatcgcagag cggttaacgg gcgtcagtga agaactcggt gcaacacgag
    11641 cacaagttgc gctggcctgg ttgttaagta aaccgggcat tgccgcaccg attatcggaa
    11701 cttcgcggga agaacagctt gatgagctgc tgaacgcggt ggatatcact ttgaagccat
    11761 aacagattgc cgagctggaa acgccgtata aaccgcatgc ggtcgtagga tttaaataag
    11821 agtccaggcc tgatgagacg tgacaagcgt cacatcaggc atcggtgcac aactacgaca
    11881 gaatacccag cggccagtga tgaccgataa agtacaggat gcctgcggaa atcaccccgg
    11941 cgacaatatc gtcgatcatg atccccatgc cgccatgtac attgcgatca aaccagcgga
    12001 tcggccacgg cttccacata tccagaatac ggaaaatcac aaacccggcg gcaacccact
    12061 gccagtcatt ggtcggcagc gccatgagcg tgatccacat accaataaat tcgtcccaga
    12121 caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt ttgatgacaa agatagacgc
    12181 cgatacagat ccccaacatc accaccagcg agtagagctg ccagggtaaa aaggtcatca
    12241 gataccagaa agggatcgcc gccagcgatc ccatcgagcc aggaacgatt gggcttaatc
    12301 cacttccgaa tccgacagca agcagatgcc acggattact catcttcagg cgacttttcg
    12361 cgacatcttt atggcgtgac aaaatggtca tatcctttcc agtcaaacgt gacaggttcg
    12421 ccgtcacgaa taaaacaaag cccttcgata tcggcggtca tttgcccgat acaggtaaac
    12481 ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac ggttcagttc cggcaccgtg
    12541 aaacacaact cgtaatcttc accgccagag agcgcccagc gcagcgcctg ttccggttca
    12601 acatggcgag aaagcgcatc agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag
    12661 tcgctggctt tcacgatatg cccgagatcg gaaatcagac cgtcagagag atcgatagct
    12721 gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac gcggcgatgg acggagatga
    12781 cgtttgatca agtagtcagc atctttagca tcggcaacct gcaaacggtt ttgcaaaatc
    12841 gccagcccgg cggcgctatc gcccggtgta ccggtcacat agatccagtc acccggtttc
    12901 gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt
    12961 gataatggcc cacgcgtggt atcgccgcca atgagttgca tatcgtaata attgagaaga
    13021 tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt ctacgtccgg taaggttaat
    13081 gccagcgtca gccaggccgg atcggccccc atcgctgcca gatcgcttag gttcaccgcc
    13141 agtgctttat aagccagatc agcaggatcg atatcaggga ggaaatggtt gcccgccacc
    13201 agcgtatcag tgctgatcgc cagggtctgt ttctcgggga tattgagaag agcgcaatcg
    13261 tcgccgatgc ccagttcgac atcaagacga gaacttctta cacggtcaaa ataacgggca
    13321 atcagggaga actcgccaca tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc
    13381 ggcgatcatg gaacagtctt ccgtgaatct accggcctgg atattacttt ttgttagggc
    13441 gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc
    13501 cgaacgattt cgccagttcg atcgcttcgt taatggccac tttgtatggc acatcactac
    13561 gtttagacag ttcgtacagc gcaatgcgca gtactgcttt ttctacctgg cccagttctt
    13621 ccagcaggcg ggacaggtag ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca
    13681 ccccggccag cagctcacgg aagtacagga cgtcaacgtc ttttacatcc tgttcagcca
    13741 ggaactggta ttcaacatca gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct
    13801 ggacggcaca ctcacgagcg cggcgacgag cagcaggttt cacggatttc cccttactaa
    13861 tttcaggcct tgatggcttt caatacatta atcatttcaa gcgcggtcag tgcagcttct
    13921 gcacctttgt tgccagcttt ggtgccagca cgttcgatcg cttgttcaat gctttcagtg
    13981 gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg caacatgcgc caggccgttg
    14041 cttgcaccac cagcgacata ttcaaagtgg gcagtgccac cacgaataac cgtacccagc
    14101 gcaatcaccg cgtcgtattt accggtttta gccagtgcac ccgccgccag cggcagctca
    14161 taggcaccag gcacccaaac aacggtaatg ttttcatctt ttacctgacc gatacgtttc
    14221 agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa agttgttgaa acgcgcgatg
    14281 gtgatggcga cgcgagcgtc cggggtagca acgttagctt caataatgtt cattttcttc
    14341 cttcgggttc gagtatggcc ccgcaggggg gcggatttta gcataatatt tcgtgcgctg
    14401 cttccctttc gagccgggag atcatgcacc cactaaatgc aggcaaacat ccgggcctac
    14461 atgacgtatc tctttgaatt taaattgggg ggcgtcggct aatttctcaa gccctggcag
    14521 cgagcataat ccacgggcgt cgctgcctaa tagtttaggt gcgatataga caatcagctc
    14581 atcgactaaa cccgcctgta gcaatacgcc agcgagcgtt ggccccgctt ccacccagat
    14641 gctgttaatc tgctgtttac ccagttgcat catcagtaca accagatcca gatgaccttt
    14701 atgctctgga atcagcaagg tacgcaccgt ttccggccac tcacgagaat cttcctgcgt
    14761 acgcgcgaac caggtttcgc cgggctgctg cacaatgcga tgttccggcg tcacgcgatt
    14821 ttggctatca atcacaatac gtactggctg gcggagattt tgttgcgggt agagcgcctg
    14881 agtttgttca tccagttcag accaacgcac tgttaaggct ggatcatccg ccagcaccgt
    14941 ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc
    15001 ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc gccgtgcggc catcaagcga
    15061 tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc atccgcttga gaaagccttt
    15121 attcaattgc tcggcttcac tcatcatcag cccgtggctg acgtcaatgc cagcctgttg
    15181 cagacggtaa agtccacgcc cagcgacctg cgggttagga tcttgcatcg ctgcgaccac
    15241 gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt ggcgtacggc catgatggct
    15301 acagggttcg agtgtcacat acgccgttgc gcctttggct ttttcacccg ccatacgcaa
    15361 cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg taaccttcac cgacaatttc
    15421 gccatctttg acaatgacgc acccgacatt cgggttgggg tgggtggtaa aacgtcctcg
    15481 ttgcgccagc tttagcgccc gcgccatgta atactcgtcc tgcacggctt agtcctccag
    15541 gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa ctgcggtaga cagaggcaaa
    15601 acggatatag gcgactttat cgagcttttt caattgctcc atcaccagat tgccaatcat
    15661 cttgctcggt acttcgcgct caccggtggc gcgcagctgc gatttaatat gattgattgc
    15721 catttcaacg taatcggaac tcaccggacg tttttccagc gcccgcagca ttccgctacg
    15781 caatttctct tcattaaacg gttcacgcac gtcgttgctt tttacaacac gcggcataac
    15841 cagctccgcc acttcaaagg tggtgaaacg ttcattacac accagacact gccgacggcg
    15901 gcgtacggat gaaccctcgc ccacgagacg agagtcaatt accttagtgt ccacggcgaa
    15961 acagaatggg caatgcatac ggcgtcctga ccaggtggtt aacggaaatc tattttaccc
    16021 tgaactgacg atacaacaaa ggcgcaacgc ttttgagata aaggatcgat gctttcgctg
    16081 cttttaccct ctaccattag gctaattgcg acgagaaagg gacgacctat gaatactaac
    16141 gtttttcgac tgctcctgct gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt
    16201 gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc aggagatgac gcaactcaat
    16261 caagaaacag tcaaaatcac tcagcaaaac aggctgaatg caaaatccag cagtggggtt
    16321 taccttctgc ctggagcgaa aacaccggca agactggaaa gccagatcgg tactttacgt
    16381 atgtcgctgg tgaatattac gcctgataca gatggcacca cccttacact acgtattcag
    16441 ggtgagtcga atgatccctt gcccgcattc agcggaactg ttgaatatgg gcaaattcag
    16501 ggaacaatag acaactttca ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc
    16561 agcgtcctcg cccccagcga tgttgatatt ccgttacaat taaaggggat ttctgtggat
    16621 cagttaggct ttgtacgtat ccacgacatt caacctgtta tgcagtaaac gtatttcggg
    16681 acgattttgt gcgtcccgca acatcttccc cgtcattttg ttactctgct tacatcacct
    16741 ggattgatag taaaagtttg caacaagggc gaaagtcagt acaatccccg cccgaatgtg
    16801 tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact gtgaaacgaa acatattttt
    16861 gtgagcaatg atttttataa taggctcctc tgtatacgaa atatttagaa acgcaatttg
    16921 cgcctttttc actcccgcaa gggattttca aacagtggca tacatatgaa aaaaacatta
    16981 ctggcagccg gtgcggtact ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac
    17041 gacaaaccgc agtatctttc cgactggtgg caccagagcg ttaacgttgt cggaagctat
    17101 cacacccgtt tcggaccgca gatccgcaac gatacctacc ttgagtacga agcattcgct
    17161 aaaaaagact ggttcgactt ctatggttat gcggatgcgc cggtattctt cggcggtaac
    17221 tccgatgcta aaggtatctg gaaccacggt tctccgctgt ttatggaaat cgaaccacgt
    17281 ttctccatcg acaagctgac caatactgac cttagcttcg gtccgttcaa agagtggtac
    17341 ttcgcgaaca actacattta cgacatgggt cgtaataaag atggtcgcca gagcacctgg
    17401 tacatgggtc tgggtaccga tatcgacact ggcctgccga tgagcctgtc catgaacgtc
    17461 tatgcgaaat accagtggca gaactatggc gcagcgaacg aaaacgagtg ggacggttac
    17521 cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt ggggcggtca gctgagctac
    17581 atcggcttca ccaacttcga ctggggttcc gatttagggg atgacagcgg taacgcaatc
    17641 aacggtatta agacccgtac taataactct atcgcttcca gccatattct ggctctgaac
    17701 tacgatcact ggcactactc tgtcgtagct cgttactggc acgacggtgg tcagtggaac
    17761 gacgatgcag aactgaactt cggcaacggc aacttcaacg ttcgctctac cggctggggt
    17821 ggttacctgg tagtaggtta caacttctga ttatgaaaat gccgggattt attcccggca
    17881 tttctgattg ttaacaacgt gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc
    17941 ggcccacaaa agcacgtaaa ttcaatatat tgctgaaata acgtaggcct gataagcgta
    18001 gcgcatcagg caattttacg ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca
    18061 gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg catcttcccc tgcgataacg
    18121 gtcgtaccat actgatccgc ttcggtatat ttcgaatgct catgatcgtg gcaatagaga
    18181 cacaacaatt cccagttact gccatcttcc gggttattgg tatggtcgtg atcaatgtgg
    18241 tgaacggtaa gttcacgcag gttagaataa acaaactcgc gggaacagcg accgcagacc
    18301 cacggataga tttttaatgc tttttcgcga tagccgcttt ctaaccgtgc atagtttttt
    18361 gggatgatag ccatgaaaag ttacctggat gaaatgtagg gttacgcgca gtttcccaag
    18421 atgcggggat tttcgcaatc tccatacatc aaaaaaaatc ccgatcttct gaccgggatt
    18481 ttcaacatca acgggaactt gattaaggca gaattgacgg ctgatccgcc ccttcttttt
    18541 ccactttctg ctgcaacatg tgttcgcgct tcatacccag tttcagagcc aacgcagatg
    18601 ctacgtagat ggaagatgca gtgccgatgg aaacaccgat aagcatggtc agcgagaagc
    18661 cttccagtac cggaccaccg aagagataca gcatcaggat aaccatcaag gtagtaccgg
    18721 atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg
    18781 taccgcgacg gatcttgcgg aagttttcac gaattcggtc cgataccacg atactgtcgt
    18841 taagcgagta accgataacc gacatcaacg atgccacaat ggtcaggtca atctcgatat
    18901 ggaataacga caaaataccc agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc
    18961 ctgccgccag tcgccactca aagcggaaac ctacgtacac gaggatagac agcagcgctg
    19021 ccatcaacgc catcgcaccg gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact
    19081 caatacgctt cactgctgca ttctgattgg tggattcgtt aatcaccttc agaacttggc
    19141 tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg catacggacc atgatgtcgt
    19201 ggctgctacc aaagttttgc agcattggct cttcaaaacc ggctttttgc aatgcatcac
    19261 gcattacgtc aatttcagcc ggtttttcga gcgtaatttc gataaccgta ccaccggtga
    19321 aatccagccc ccagttaaag ccgcgcacgc ccataataac gatagcagcg attaacagca
    19381 gaccagagat gccgaaagcc cagtagtccc agcgcataaa gtcatagact ttacggccgt
    19441 ggtttagttg ttcaacagta tattcctgtg ccacatcgca ctcctcagat tgacagcttc
    19501 ttgacgcgct tgccgccata tagcaggttt acgatggcac gcgtaccgac aatcgcggta
    19561 aacatcgacg tcgccacacc gataccggta gtaatcgcga accctttaat tgccccggta
    19621 cccactgcgt acaggatgat gactttaatc agcgtggtga tgttcgcatc gaagatagaa
    19681 ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac
    19741 tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga ccgctaccgc aagggttaag
    19801 acgatacccg cgatacctgg catactcagt gttgcgcctg gcaacagcga cataataccg
    19861 acgatcaaga tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa cttcttatag
    19921 aagatgatca tgaacagaat agaaaccagc agaccggcaa ggcaagcctc cagcccctgc
    19981 tcaatgttct gcatacccag ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc
    20041 gcgatcaacg caccggcacg cagcagcagt gacagctgac gggcttcgtt cgggttgttg
    20101 atgccggtga tacggaagct gttacccaga cgagactgga tgttggcgat gttaatcacc
    20161 tcttcctgtt tcaccagaac cgcacgacca tttgcatctt tcttaccgct gtctttgtac
    20221 tccacaaaca gggttgccat cggtttgccg atgttgtcct tggtgaagtt agacataatg
    20281 ttgccaccag cgctatcgag cgagatgtta acctgcggct ggttgtattc gtcctggctg
    20341 gaagtggagt cggtgatatg gtcaccagtc agaattacgc gtttgtacag cacaaccggc
    20401 tgaccttcgc gggtctgttt cacttcagag tcgcccggta cgcgaccgga tgccgcagcg
    20461 gcctgatcaa cattggtgtt taccagacgg aattccagcg ttgccgtcgc gcccagaatc
    20521 tctttcgcac gcgcagtgtc ctgaataccg ggcagttcaa caacgatacg gtcagcaccc
    20581 tgacgctgaa ccaccggctc cgccacgcca agttggttta cacggttacg caggatatta
    20641 atgttctgct gcaccgcata ttcacgcgct tcactcagac gagcatcgct cattaccgca
    20701 cgcagctggt tgctgccctg gctgctaatc accaggtccg gatggcgctt gctcagatac
    20761 gcaatggctt catcacgagc tttagcatcg cggaaagtga tgctcaggcc gtagttgttt
    20821 tctttacgaa cagtggtata cgggatgccc ttttcgcgca ggtcactgcg taggctatcg
    20881 atattttgtt cctggagttt gccaagcgcg gtgtccatat ccacttccat caggaagtga
    20941 acgccgccac gcaggtcaag gccgagcttc atcggctcag catgaatagc tgccagccag
    21001 cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt tgtcacccat aacgcccatt
    21061 aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc
    21121 tcttccagtg ccacagactt agcagttatt ttttcttctt gtaacgtttt ctggacctgg
    21181 atcagcgttt gctcactggc ggcgactccg cgcgcaccag tgatctgaac agccggatcc
    21241 tcaccaaaca ggttgggaag cgcatacagc agaccgatga caatcaccac gatcagcatg
    21301 acgtacttcc acaaaggata acggtttaac acggcaattc ccttagggaa aaattttaat
    21361 tacagcgcct tcatggtgcc tttcggcagg acggcagcta cgaagtcacg tttaataact
    21421 acttcagtgg tgtcattcag cgcgatagca atgtagccgt tttccgctac tttggttacg
    21481 cgaccaacca ggccaccgtt cgtcagaact tcatcacctt tggcaatgga gtccatcagc
    21541 tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga tcatgaaata gaaaatcaga
    21601 ccgaatacca ccagcatcaa aatcaaagac atcgggctac cttgcgccgg tgcacccgtt
    21661 gccgctaccg catcagaaat aaaaaagctc attaaatttc cctcattatt aatattaatc
    21721 aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa aaatcagtta cgaagctctc
    21781 taatttaccc tcttcaatag ccttgcgtaa acccgccatc aaacgctggt agtaacgaag
    21841 gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa
    21901 gtaagcgcgt gaataattgc gacaggtgta gcaatcacac tcaggatcga gtgggccagt
    21961 atcgctctta tatttcgcat tgcggatttt caccacgcca tcggtcacga acaaatgacc
    22021 atttcgggcg ttgcgggttg gcattacgca gtcaaacata tcgataccgc gacgtacgcc
    22081 ttcaaccagg tcttccggtt taccaacgcc catcaggtaa cgcggtttgt ctgccggaat
    22141 ttgcggacac acatgctcca gaatacggtg catatctgct ttcggctcac ccacagccag
    22201 accgccgaca gcgtagccat caaagccgat atctaccaga cctttaacag atatatcacg
    22261 taaatcttcg taaatactgc cctgaattat accaaataac gcatttttgt taccaagact
    22321 gtcaaaacgc tcacggctac gcttcgccca acgcagagac atctccatag agcgttttgc
    22381 gtaatcccag tcagcaggat acggcgtaca ctcatcaaag atcatgacga tatccgaacc
    22441 aagatcgtac tgaatctcca tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat
    22501 cgggttacgg aagtgaacgc cctgttcggt gattttacga atatcaccaa ggctgaagac
    22561 ctggaagccg ccggagtcgg taagaatcgg tcctttccac tgcataaaat cgtgcagatc
    22621 gccgtgcagt ttcatgattt cctggcccgg gcgcagccac aggtggaagg tgttgccgag
    22681 gataatttgc gcgccagtgg cttcaacttc ttccggcgtc atccctttta cggtgccgta
    22741 ggtgccaaca ggcataaaac aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc
    22801 acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc attttttctc cactacgtca
    22861 gaaaaacagt ccaacgttta aaccagcgcc gcggaattac tccccgacgc gctcattaat
    22921 tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc
    22981 ttctaccgcc gctttatagg cattcatggt gtgttgataa ccggcaaagg ccgaaaccag
    23041 cataatcagc gtcgactctg gcaagtggaa gttcgtcacc agcgcatcga ccactttgta
    23101 ctggaagccc ggatagataa agatttgggt atcgtcaaag aacggttcga taagatcgtt
    23161 ttttgccgcc tgtgccgcgc tttccagcga acgtactgaa gtggtgccaa ccgcaatcac
    23221 ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg gtacttcagc
    23281 gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc
    23341 cgcaccaacg tgcaacgtca caaacgccat ctccacgcct ttggcgcgta atttttccag
    23401 caaaggctcg tcaaaatgca gaccagcggt cggggctgca accgcgcccg gtttttcgct
    23461 ataaacggtt tgataaagtt cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg
    23521 cagcggcata tggccgatgc tgttgaggat atccagtacc gagcgttcat cattaaattc
    23581 gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc
    23641 cagcaacagt tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg
    23701 tttgtcgtcg agcatccgct caaccagcac ttcaatcttg ccgccgctgg ctttacgccc
    23761 aaacagacgc gccgggatca cgcgggtatt attaaaaacc agaagatcgc cggggttgag
    23821 cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga
    23881 cagtaaacga cagctactgc gttcaggcat gggatagtgg gcaatcaggg attcgggcaa
    23941 ttcaaaggag aaatcggtaa cgcgcatgac actgactcag actaaaataa gagtcgggta
    24001 gtctagtgcc ggggcgctct ccctgcaacc tttacccccc tttggataga atataatcga
    24061 tgaatttttt agctcacctg catttagccc atctcgcgga aagctcgctt tctggcaatt
    24121 tactggctga tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg
    24181 gcattcatat gcatcgacgt attgactgac aatctgccgg aagtccgcga agcacaggag
    24241 tggtttcgta gtgaaacgcg ccgcgttgcg cctattacgc tggatgtcat gtgggatcac
    24301 tttctttccc gccactggtc gcagctgtcg ccggactttc cgctacagga atttgtctgt
    24361 tatgcccgcg agcaagtgat gacgattttg ccggactcac cgccacgttt tatcaatctg
    24421 aacaattact tgtggtcaga gcagtggctg gtgcgctatc gcgatatgga tttcatccag
    24481 aacgtgttaa acggcatggc aagccgccgc ccacgtctgg atgccctgcg tgactcctgg
    24541 tacgatttag acgctcatta tgacgccctc gaaacccgct tctggcagtt ttatccgcgg
    24601 atgatggcgc aggcgtcaca caaggcgtta taaaacgtta gttcatccat accgtagccg
    24661 aaatggcagg caaagccaga atcccgtcag tcagttgccc atgcccttct ttgcattgcc
    24721 attgcacggc attgagaaac ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt
    24781 tgattgccac cagtacacgt tgctgattca gcacgcggac aaataccact acgttatctt
    24841 ccgcatacag cacctgacag ccgccacgac gcagcgcctg acttttctta cgcagcacaa
    24901 tcattcgccg gtacagcgcg aataacgccg tatcctgctt ttccacctgc caggggaacg
    24961 gtttacggca aaacggatcg tttttaccat ccagtcccac ttcatcaccg taataaatgc
    25021 acggtacacc aggccaggtg aacagccaga ccaccgccag cggcaggcgc gcaatatccc
    25081 gaccgagcag cgttttaaat cgcgcagtat cgtggctatc gagctgatta aacatacgta
    25141 attgttgttg atgagaaagc cctgcgcggt aattatccat ccaggccata caggtttggg
    25201 cgtcaatttg ctgcggatca taagagatgt cggtattggc aagaaatccc cacaacggga
    25261 atgtgaagcc acgatagttc atggcagcat cttccacatc ggcctgtaac cattgccgtg
    25321 catcgccaaa atgttcgccg acaatatacg cttccggctg ggtttctttc gccgcttcgg
    25381 tgatcccggc aacgtgctgc atattattgc gcgccccacc cgcctccccc agcatatgca
    25441 ccacatccag ccgccagccg tccatattcc acggcgcttt cagccagtgg cggacaatac
    25501 tgtcttcccc gcgataaatt tcattcacca gactttccga ctgataatcc agcttcgtca
    25561 agctggcata gccaagccag tcgagcgccg tgccatcatc actaaacgag taccagtcgc
    25621 gccagggcga ttcagagttg tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc
    25681 aggcatggga atcgccactg tggttaaaca cgccgtccag caccagccgc attcccagct
    25741 gctgcgtatt gtgtcgcaaa cgcagcaacg ccccatcacc gccaaactgc ggatcgacat
    25801 ggcgataatc ctcggtatcg tatttatgta cgctgggagc tttaaacacc ggattgagat
    25861 acagcgctgt cacgccaagc tttttcagat acggcagttt ttcgcttatc ccgtccagat
    25921 cgccgccata gaacgttgat ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca
    25981 agatgatctc ttgtccggct gcatgatggt aatagacatg atcctgttca gcttcacgag
    26041 gaagactacg cgcaaaacga tcagggaaga tctgataaaa aatctgatcc gcagcccatt
    26101 gtgggccgat atcaggtaca tcgacggcaa actgctccag tcgtgccggc ggcattcggc
    26161 tgaagccctg cggtgtaaac caacgctggc gatcgtgcca cagcagtttg aaactgtaac
    26221 gccgccgggg ttgtccgctg gagagatcaa tcgccgcacg ccatgcggtg acgcctggct
    26281 gcggctgact gcgctgctta tgcattggta ctgacatttc ttcgttatcg tgttctgtac
    26341 gcagcataat gcgctgcggt gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat
    26401 ctttgctttg tttaacaaat gggggcaccg gcaggtgcca tgcatttaac atcataattc
    26461 ccctgtgatg aaaatctgat caccttgcca tagcctaatg caggatcact catcatctga
    26521 aggatatttg ggggaggagg acgggggcgg ataactttcg gaatagccgg atcaggattt
    26581 cctcctgaac cggcttacag aacgcaagta ttattggttt tcagccagct gacgatcgtg
    26641 gcggcgttta aacatccagc caatcaacag cacaacaatc cacgcgaaac cgacatacag
    26701 cgagatacgc gtatccgggt gataaccaat caacccgata ataaagagca ggaaaatcag
    26761 cccgccgatg gtcgttgcta ccccacccgg cactttaaat ttcagcgcct taacttcttc
    26821 tggcggcaaa cggcgacgga aggcgatttg cgacagcagg atcataatcc acacccatac
    26881 cgtggcgaag gttgccagcg aagcgatcac caggaagacg ttttccggca tgatgtagtt
    26941 cagatacacc gcaaacagca gcgcggtagt catcaccagc accgtaaccc acggaatacc
    27001 gcgacgcgac gttttgctga aaattttcgg tgcgctgccc tgctctgcca taccgtggag
    27061 catacggcct acgccaaata cgtcactgtt aattgccgac agcgaagcgg tcagcacaac
    27121 aaagttaaga atgctggcgg caaaggtaat gcccatatgc tggaacgtca gtacgaacgg
    27181 gctaccggca gtgccaacct gattccacgg gtagatagac ataatgacga acagcgtacc
    27241 gacgtagaac accagaatac gcatcggcac ggagttaatc gcacgcggaa tcgatttctc
    27301 aggatctttc gcttcaccgg cggtaatccc gataatttcg atcccaccgt aagcaaacat
    27361 caccatttgc aacgacatta ccatgccaag ccagccgtta ctgaagaagc cgccgttgct
    27421 ccacaggtta tgaataccgg tcggttgccc gccgttgcca atcccccaga tgatgatgcc
    27481 gaaaccggcg acaatcatga tgatgatggt ggcgacttta aagaacgaga accagaattc
    27541 cagctcaccg aagaccttca cgctcatcag gtttacggcg caaatgatca gcaccacgct
    27601 cagtacccaa atccagtgcg gcaccgtcgg gaacaagaca cccatataga taccaaaagc
    27661 ggtcacatcg gcgatggcga caataaggat ttcaaaacag taggtccatc cggtaatgta
    27721 acctgccagc gggccaaggt tttcctgcgc ataacgcgag aaagagctgg cggccgggtt
    27781 atgtaccgac atttccccca gcgcacgcat aatgatatac gccgcgatac caccgataat
    27841 ataggccaac aacacgctcg gaccggccat tttgatggcg tctgccgaac cgtaaaacag
    27901 cccggtgcca attgctgaac ccagtgccat aaagcgtatg tggcgggtac ttagcccacg
    27961 ctttagctta ttcttacttt ccatcaattc ctaacccatc aacacaataa aaaaccacgg
    28021 ggcatcgcag ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac
    28081 ctgacgacct gccgcgcgat cccagataat ggccagaacc accatcacca ctgttggcat
    28141 taaccacgcc agaccttgtt cggccagcgg taaacgctgc gcccaggacg gtaagatatc
    28201 gctgaatgca gatgctttga tcccgtcgag aataccaaaa agcaggctga taaacatcgg
    28261 cggagcaatc acgcgggacg aattatgcca ccatgagcgt gtaaaactta atacaaccag
    28321 tgcgatacac ggcggataaa tagcggtcag caccggtacg gagatctgga tcagctggct
    28381 taagccgagg ttagacacca ccatcgagaa gccgccgagg ataaacacca gcgtacgata
    28441 agagagcggt acgtactggg caaagaattc tgcacaagca caggtcaggc caaccgccgt
    28501 gaccagacag gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt
    28561 gtgctgaacg taagcatgca gaatagcagc gccgtttgca gactgatcga ccagcgacgc
    28621 gctgtctgaa cccagacaga acagcgccag gtacagcaaa gtcagaccaa cacccgccat
    28681 caggccagcc cagacggtat aacgggtcag cagacgcgct tcggtaacgc cacgagaacg
    28741 cgccgcgtta acaataacga taccaaacac cattgcgccc agcgtatcca tggtcagata
    28801 gccgttaacg aagccgttag aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat
    28861 agaacccgcc ggccagacaa tcgcgacaac agacaggatg accagcgcga taattttcag
    28921 cggcgcaagg aagttgccca cggtatccag cagcttgccc ggatagagcg aaaccagaat
    28981 aacgatagcg aaatagacca ggctgtagat aaacagcggc agcgcggaat cacccgtcag
    29041 cggcgcaatc cccacttcaa aggaaacggt agctgtacgc ggcgtagcga aaagcggccc
    29101 caccgccagg taacaaaccg ttgccagcag tacgccagcg actttaccga ttggcgtgct
    29161 gaggctgtca acaccgccgc caacttttgc cagcgccact accgttaata ccggcaggcc
    29221 aacggcagta atgaggaagc cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc
    29281 gaccattgga gggaaaataa tgttacctgc gccgacgaac aacgcaaatg tcataaagcc
    29341 cagagcgatg atatcgcgcg atcttaattg atgggtcata aatcatactg cctgtggatg
    29401 tggtgttgta acgttaaatt tttgtgccaa tccctgggga tgatacagcg gtatatttaa
    29461 acaattttag cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc
    29521 gcgttaatgc agtcagtatg acagcacctg aagcgcaatt taaacgctta taacgtttta
    29581 aagcaaggag ggatctaaaa accagaggta tatagcgata agaaatcacc aaccagtata
    29641 aaacactatt tccgtgaaaa agacatggct aatcatgcga acaaaaaatc accaaccgtt
    29701 gtaaaatcta acgcccgtat tttgactgca aagaaaataa gccagcatcg gctggcttat
    29761 ggcaataaaa gatgacaaag gcggattaat cgctgttttt ggcaattaaa cgttccggga
    29821 taacaaaact gaaacgtgtt ccttttccta ctgtactctc aatattcagg cgactttcgt
    29881 gatgattcac cgcatgtttc acgatcgcta accctaatcc gctaccgccg gtttgccggg
    29941 aacgcgcttt atcaacgcga taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa
    30001 tgcccggtcc gttatcttca acgctaaatt cggcaccgtg cggcactcgc tgccagcgta
    30061 cggtgatatg cgtgccttcc ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg
    30121 cactgcgtag ctgatcttcg ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa
    30181 atgtctgttt tttctgactc agagtctgag cctcgcgctc aacaacgcgc agcatcatcg
    30241 gcacatcaac cttttcattg agcaattgcg tcggcgcggc ttcaattttc gacagcgtta
    30301 gcaactgctt caccagccct tccatccgct gggtctgctc gcgcatggtg tgcaacgctt
    30361 tttcgcgtac cgcgccttcc agcggctgct catccatcat ctccaggtaa ccctgtaaca
    30421 cggtcaatgg cgtacgtaac tcatggctca cgttggcaaa aaagttacgc cgcgcccctt
    30481 ccagttgatg catttgcgtg acatcacgcg ccaccatcag caactgtttg tgggtataag
    30541 gcatgacgcg aatttccaga tgccgcccgg tgttgagcac cagattgagc gggcgagaaa
    30601 aatcacgcgt tttcagatat tgcgtaaact ccgggtaacg cagtaggtta aggatgttct
    30661 gcccgttatc ttccggccag cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga
    30721 aaataccgcc ctcttccgtg gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac
    30781 gaaagcgttt aatcagattg cccagttcac ggcggcgttt tttatttcgc agctgcatct
    30841 ggtgtaagcc gtatagtagc ggttcccagc taccacgccc cggtggcggg gtcatactgc
    30901 gatccaccca cagccaccat gaaaggcgca ataaattcca gaaatgccag ataagcagtc
    30961 ctgttaccga tgccagcaaa aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag
    31021 ccgggaggca gcaaagtagc agctccagca ccagcctttt ccacgacagc cgttccagca
    31081 cgtaagatac tccagttaag aaatcataag ccctgctctg cgtccgatga gcaaggcgtt
    31141 aaaagcgggt tgaaaaacga tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc
    31201 cgccgggctc cagtgcttta cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca
    31261 catacacgtt agttccccag acgtggttta acagctgctc gcggctgtac acgcgctcag
    31321 gatgcgtcat aaaaaagtgc agcagtttaa attctgtcgg ccccatctcc agcggctctt
    31381 cgcccgccat cactcggtga gatgtcgggt tgagacttaa tccctgcatc ttaatcacct
    31441 cttccaccgc cattggcgaa atacggcgca ttaccgcttt gattcgcgcc accagctcct
    31501 tcggcgaaaa tggcttggtg atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat
    31561 cttcttcttc ccctctggcg gtcaacatca ccactggaat atcccgggtc atcgactcgc
    31621 gcttgaggtg tttgatgaac tggataccgg agccgccagg taacatccag tcaaggagaa
    31681 ttaaatccgg ccagggttca ttcagttgat tcacagcact gtcataatct tccgcttcga
    31741 ccggctgaaa gccattttgt tcgagcacga agcagaccat ttcgcgaatt ggagcttcat
    31801 cttctacgac cagaatacgt ctcgccatga tttgccctgt tgtaataaat aggttgcgat
    31861 cattaatgcg acgtcattat gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac
    31921 atatcttcag gttattgatt tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg
    31981 tataatcgcg accatgcttt ttcgccaggg aaccgttatg cgcatccttc acacctcaga
    32041 ctggcatctc ggccagaact tctacagtaa aagccgcgaa gctgaacatc aggcttttct
    32101 tgactggctg ctggagacag cacaaaccca tcaggtggat gcgattattg ttgctggtga
    32161 tgttttcgat accggctcgc cgcccagtta cgcccgcacg ttatacaacc gttttgttgt
    32221 caatttacag caaactggct gtcatctggt ggtactggca ggaaaccatg actcggtcgc
    32281 cacgctgaat gaatcgcgcg atatcatggc gttcctcaat acaaccgtgg tcgccagcgc
    32341 cggacatgcg ccgcaaatct tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc
    32401 cattccgttt ttacgcccgc gtgacattat ttccagccag gcggggctta acggtattga
    32461 aaaacagcag catttactgg cagcgattac cgattattac caacaacact atgccgatgc
    32521 ctgcaaactg cgcggcgatc agcctctgcc catcatcgcc acgggacatt taacgaccgt
    32581 gggttccagt aaaagtgacg ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc
    32641 ggcacaaaac tttccaccag ccgactacat cgcgctcggg catattcacc gcgcacagat
    32701 tattggcggc atggaacatg ttcgctattg cggttccccc attccactga gttttgatga
    32761 atgcggtaag agtaaatatg tccatctggt gacattttca aacggcaaat tagagagcgt
    32821 ggaaaacctg aacgtaccgg taactcaacc catggcggtg ctgaaaggcg atctggcgtc
    32881 gattaccgca cagctggaac agtggcgcga tgtatcgcag gagccacctg tctggctgga
    32941 tatcgaaatc actactgatg agtatctgca tgatattcag cgcaaaatcc aggcattaac
    33001 cgaatcattg cctgtcgaag tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt
    33061 gttagccagc caacagcgtg aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg
    33121 ccgtctggca ctggaagacc tggatgaatc gcagcagcaa cgtctgcagc atcttttcac
    33181 cacgacgttg cgtaccctcg ccggagaaca cgaagcatga aaattctcag cctgcgcctg
    33241 aaaaacctga actcattaaa aggcgaatgg aagattgatt tcacccgcga gccgttcgcc
    33301 agcaacgggc tgtttgccat taccggccca accggtgcgg ggaaaaccac cctgctggac
    33361 gccatttgtc tggcgctgta tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa
    33421 aatgatctca tgacccgcga taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa
    33481 ggtgaagcgt atcgcgcgtt ctggagccag aatcgggcgc gtaaccagcc ggacggtaat
    33541 ttgcaggtgc ctcgcgtaga gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa
    33601 gtgaaagata agctggaact gacagcgacg ttaaccgggc tggattacgg gcgcttcacc
    33661 cgttcgatgc tgctttcgca ggggcaattt gctgccttcc tgaatgccaa acccaaagaa
    33721 cgcgcggaat tgctcgagga gttaaccggc actgaaatct acgggcaaat ctcggcgatg
    33781 gtttttgagc agcacaaatc ggcccgcact gagctggaga agctgcaagc gcaggccagc
    33841 ggcgtcacgt tgctcacgcc ggaacaagtg caatcgctga cagcgagttt gcaggtactt
    33901 actgacgaag aaaaacagtt aattaccgcg cagcagcaag aacaacaatc gctaaactgg
    33961 ttaacgcgtc aggacgaatt gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag
    34021 gcgttagccg aagaagaaaa agcgcaacct caactggcgg cgcttagtct ggcacaaccg
    34081 gcacgaaatc ttcgtccaca ctgggaacgc atcgcagaac acagcgcggc gctggcgcat
    34141 attcgccagc agattgaaga agtaaatact cgcttacaga gcacaatggc gcttcgcgcg
    34201 agcattcgcc accacgcggc gaagcagtca gcagaattac agcagcagca acaaagcctg
    34261 aatacctggt tacaggaaca cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg
    34321 cgtgcgcagt tctcccaaca aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag
    34381 ttaacccatg ctgagcaaaa acttaatgcg cttgcggcga tcacgttgac gttaaccgcc
    34441 gatgaagttg ctaccgccct ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg
    34501 gtcgcgctgc atgggcagat tgttccccaa caaaaacgtc tggcgcagtt aatggtcact
    34561 atccagaatg tcactctgga acagacgcaa cgtaatgtcg cacttaacga aatgcgccag
    34621 cgttataaag aaaagacgca gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg
    34681 cgcatcaaaa cgctggaagc ccagcgtgca cagttacagg cgggtcagcc ttgtccactt
    34741 tgtggttcca ccagccaccc ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat
    34801 cagtctcgat tactggcgct ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg
    34861 ctgcgcgggc aactggatgc attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa
    34921 agcctccgac aagatgagca agcacttact caacaatggc aagccgtcac ggccagcctc
    34981 aatatcacct tgcagccaca ggacgatatt caaccgtggc tggatgcaca agatgagcac
    35041 gaacgccagc tgcggttact cagccaacga catgaattac aagggcagat tgccgcgcat
    35101 aatcagcaaa ttatccagta tcaacagcaa attgaacaac gtcagcagca gctcttaacg
    35161 gcattggcgg gttatgcact gacattgcca caggaagatg aagaagagag ctggttggcg
    35221 acacgtcagc aagaagcgca gagctggcag caacgccaga acgaattaac cgcgctgcaa
    35281 aaccgtattc agcagctgac accgattctg gaaacgttgc cgcaaagtga tgatctcccg
    35341 cacagcgaag aaactgtggc gctggataac tggcggcagg ttcatgaaca atgtctcgca
    35401 ttacacagcc agcagcagac gttacagcaa caggatgttc tggcggcgca aagtctgcaa
    35461 aaagcccaag cgcagtttga caccgcgcta caggccagcg tctttgacga tcagcaggcg
    35521 ttccttgcgg cgctaatgga tgaacaaaca ctaacgcagc tggaacagct caagcagaat
    35581 ctggaaaacc agcgccgtca ggcgcaaacg ctggtcactc agacagcaga aacgctggca
    35641 cagcatcaac aacaccgacc tgacgggttg gctctcactg tgacggtgga gcagattcag
    35701 caagagttag cgcaaactca ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt
    35761 cgccagcagc tgaagcagga tgcagataac cgtcagcaac aacaaacctt aatgcagcaa
    35821 attgctcaaa tgacgcagca ggttgaggac tggggatatc tgaattcgct aataggttcc
    35881 aaagagggcg ataaattccg caagtttgcc caggggctga cgctggataa tttagtccat
    35941 ctcgctaatc agcaacttac ccggctgcac gtgcgctatc tgttacagcg caaagccagc
    36001 gaggcgctgg aagtcgaggt tgttgatacc tggcaggcag atgcggtacg cgatacccgt
    36061 accctttccg gcggcgaaag tttcctcgtt agtctggcgc tggcgctggc gctttcggat
    36121 ctggtcagcc ataaaacacg tattgactcg ctgttccttg atgaaggttt tggcacgctg
    36181 gatagcgaaa cgctggatac cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa
    36241 accatcggtg tgattagcca cgtagaagcg atgaaagagc gtattccggt gcagatcaaa
    36301 gtgaaaaaga tcaacggcct gggctacagc aaactggaaa gtacgtttgc agtgaaataa
    36361 ctattcagca ggataatgaa tacagagggg tgaattatct cttggccttg ctggtcgtta
    36421 tcctgcaagc tatcacttta ttggctacgg tgattggtag ccgttctggt ggttgtgatg
    36481 gtggtatgaa aaaagtcatt ttatctttgg ctctgggcac gtttggtttg gggatggccg
    36541 aatttggcat tatgagcgtg ctcacggagc tggcgcataa cgtaggaatt tcgattcctg
    36601 ctgccgggca tatgatctcg tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg
    36661 cactcttttc cagccgctac tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg
    36721 tcattggcaa cgccatgttc acgctctctt cgtcttacct gatgctcgcc attggtcggc
    36781 tggtatccgg ctttccgcat ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa
    36841 ttatcaaacc cggaaaagtc accgccgccg tggcggggat ggtttccggg atgacagtcg
    36901 ccaatttgct gggcattccg ctgggaacgt atttaagtca ggaatgctgg cgttacacct
    36961 ttttattgat cgctgttttt aatattgcgg tgatggcatc ggtctatttt tgggtgccag
    37021 atattcgcga cgaggcgaaa ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg
    37081 ccccgtggtt aattttcgcc gccacgatgt ttggcaacgc aggtgtgttt gcctggttca
    37141 gctacgtaaa gccatacatg atgtttattt ccggtttttc ggaaacggcg atgaccttta
    37201 ttatgatgtt agttgggcta gggatggtgc tgggaaatat gctaagtggc aggatttcag
    37261 gacgttattc accactgcgc attgcagcag tgactgactt tatcattgta ctggcactgc
    37321 tgatgctctt tttctgcggc ggcatgaaaa caacgtcgct tatttttgct tttatttgtt
    37381 gcgcgggatt atttgccctt tcagcacctc tgcaaatatt gttactgcaa aatgccaaag
    37441 gaggagagtt attaggtgcc gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg
    37501 gcgcatattg cggaggtatg atgctgacgc tggggctggc atataattac gtggcgctgc
    37561 ctgccgccct gctttcgttt gctgcgatgt catcgttgct gctgtatggt cgctataagc
    37621 gccagcaagc ggcggatagt ccggtgctgg cgaaaccact ggggtagatt actgtcacgg
    37681 tgcgcgttgc ttattgccgg atgcggcgtg aacgccttat ccgccctacg cggttctggc
    37741 acattttgta ggcctgataa gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc
    37801 ctacgttttt tactcttgtg gccataacca cgcagcgccg cgtacgccgc tggaatcacc
    37861 gtgcttcgcc ttactcaccg gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa
    37921 ctgcccaacc gtttgatata aacggtctac attgctcatc ccgcccccca ggacaatcac
    37981 atccggatcg agaatattca cgacatgtgc cagcgatttt gccagccgca gctcgtagcg
    38041 acgcaatgcc agttccgcta ccggatcgct ttcttcaacc aggcggataa tttcactgcc
    38101 tttcagcgca tgtccgctca aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa
    38161 ggtttcaata caaccttgtt taccgcaata acaagggact tcctcgcgat aacgcagttc
    38221 gtcttcgtcc atccacggta gcggattgtg tccccactca cctgccgtgc cattgccgcc
    38281 gatatgcgtc cgcccattga atgccacgcc cgcgccgcat cccgtgccga taatcacggc
    38341 aaataccgtc tgcgctcccg ctgccgcgcc atctactgct tctgaaaccg ccagacagtt
    38401 ggcatcattt gccagccgca cttcccgctg caacctcgcg cttaagtctt tatcgaatgg
    38461 ctgaccgttg agccaggttg aattgacatt cttcaccaca ccggtgtagg gcgaaatcga
    38521 acctggaatg cccataccta ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac
    38581 caacgtggcg atcgtttcaa tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg
    38641 gcggtacaac tgctcccctg catcgcccag tgcaatcact tcagttttgg tgccgcctaa
    38701 atcgatacct atacgcactg tactctcctt atttttttca atatcaatag cgtagagacg
    38761 gacaaccgga ttggcaatgc aaggccgccg acaattcgtt atcatgcccg ctaaatttaa
    38821 cgacaaggcc gtggaaatta tcatgctgtg gttcaaaaat ttaatggttt accgtcttag
    38881 ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag ctagcctcga tggcatttac
    38941 cccatgcggc agccaggaca tggcgaagat gggctgggtt cctccgatgg gatcgcacag
    39001 cgatgcgtta acgcacgttg ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa
    39061 aatcctcccg tctccggtga ttaaacaggc gctggaagcg aaaatcgcca aactggaagc
    39121 ggaacaggcg cgtaagctga agaaaaccga aaaagattcg ctgaaagacg aagtgctgca
    39181 ctctctgctg ccgcgtgctt tcagccgttt tagccagaca atgatgtggc tcgacacggt
    39241 taacggtttg attatggtgg actgcgccag tgccaaaaaa gcggaagata cgctggcatt
    39301 actgcgtaaa agcctggggt cgttaccggt tgtaccattg agcatggaaa acccgattga
    39361 actgacgctg accgaatggg ttcgctccgg tagtgcggca cagggcttcc agctgcttga
    39421 tgaagccgag ctgaaatcgt tgctggaaga tggcggcgtg atccgcgcga agaaacaaga
    39481 tctgaccagc gaagagatca ccaatcacat tgaagccgga aaagtggtga ctaaactggc
    39541 gctcgactgg cagcagcgca ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct
    39601 taagttctgc gacgagctgc gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca
    39661 gcgttttgat gccgatttca tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct
    39721 gattgaagga ttaggtggcg aagcacaacg ttaattgctg attttccttt aatgccggat
    39781 gcgacgcctg ccgcgtctta tccggcgtac gaagccacac caggcatata attattcgct
    39841 acggcgagca ataattttta gcgcagcaat attatgcgtt ttacgccgta actgatctaa
    39901 tcgcctcatc cctattcgcc tgtcagcagc acgcagaata ttcactaacc cccatttatc
    39961 gctggtaaat tgttttgtta acagctccgc ttttggggta tcaatatatt cacgcaataa
    40021 atcagaaatt gaacaaatat ccgtcaggta aggataccag caagtttcac cgtgaaaatt
    40081 acgcactcca gcgttacctg caataaagtc tttcgggtaa tcccagatca cgtttttatc
    40141 caacgtgatc caataacgcg gtaaatcggt acttccacca ttttggctgc gcatgggata
    40201 acgggtacag tgaatttgca ggttgatcgt tggcgtcagt aagtcataga tctcccgctg
    40261 taacttgctc catggacgtt gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg
    40321 cgaattttcc gcgttgcccc ggttttcact aaatcacgta actgcgaaag atcgggttgt
    40381 tcgacaaata agagtaatga tggatcctct tttaaccacg ccgttttcac atcggcagcc
    40441 agctgtggat tattaatagc cagcgaccga tcctgaggct cagtcaatgt cgttaacaac
    40501 gacgccgggg tatgtgcgtt gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc
    40561 agcaatgcac gccaccattc atcatctgat ttatctttta tcgtagccag ttccgcaaaa
    40621 aatacctgga gatcttcact gatatttata tcaaccttga tgaattatga atatatcgga
    40681 aattgaaaaa atcgcagcaa aggaaatatt attatgggtt aaagagagga tatttcagaa
    40741 aaatgccctc ccgttcaagg ggaaggcgag gaattacaga tagcggcaca gataagaggt
    40801 aggttcggca acttgaagat gaaactcact gtgaccggga acattaaaca ccgaaccggc
    40861 ttcatacact tgccagtcgg tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac
    40921 cgtcatctct tccggctcag cggtgctgaa ggtgtattcg ccttcaacca taacacccac
    40981 gctggcgcga ccagtgctgc tgctggaaaa gccgatcgat ttcactttgc cggaaaagta
    41041 ctcattactt tgaagcataa actggccctg tctggtagtt gaacatctac ttcactatag
    41101 gggccagagg cgctggctgt cacgcaaaat tacaccagta attccgcagc cagccgtgca
    41161 atcaatacgt tagagagcaa gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga
    41221 cgttggtgaa atcctaaaca atccagcatg atgacatcag ccccttttgc cagtaattct
    41281 ttcccagcat caatgatttt ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc
    41341 ggcgatttct gcaaaatttg ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga
    41401 acgataaccc ccacctgatg atcttcaaca atagaggaaa ccagtggagg caatattcgc
    41461 gacggctcaa gaaagatcgt attacgcgca gtcatactac taatgtttgc tgtactcatt
    41521 aataaaatga cgtcataacc ctgattatcg agcacttcaa ccacaccttg cagatctcgc
    41581 tccactttac gacgcgaaac atgggccagc tggttgtcat ttaataatgt gagaatggtg
    41641 tcttcgcctg cttctggcgc gtactccgcc atcacttctt cacgacttaa cttccccagc
    41701 aggctatgat gggaaatatt atcttcgtca atgtattccg tcaggagcgg caaaacctcc
    41761 tgcataggta caatgccgat ggtcaggatc gccaacgacg cactcattgt tcatccacct
    41821 tacttttctt tcgtcgttaa ttaccgggca agtgtgaagc accactgctg acacttactt
    41881 gtgctacaaa tgacaaaaag cgtagcagca gacgtgcacg gcataatgta aagattccaa
    41941 atgattccag taatggaatg ttattgttta atattctaat tattagaaaa acataaatta
    42001 tgaaaaatgt gacgcagatc ccttgtagtg gcactcagaa tcccttccga gcagtctgat
    42061 gcggtcactc tttgtcaggg tcctcatagc gttttttcgc atccatcgct tcctgagccg
    42121 tcggatgttc actgatcaac gagtctggtt taggatgatc ggctctgagt tggtaccagg
    42181 ttaccgtctg tcctggcttt cctttttcaa tcgtcactat atatgcttca cgaggataag
    42241 gcggtttcgt tggcatcgtt cttccttatt tcacgggatg aacgttaagt ataggcgctc
    42301 taaaatcaac aattgatcgt ctgtgccagg gcgctacgaa tttcagaaat cacctggctg
    42361 ggttcgtttg ttgcgtcgat gataatatgc gcaacttcgc gatatagcgc atcgcgttct
    42421 tccagcactt cctgaacttc ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct
    42481 tcttccggtg cagcttgcag tcggttaacc aggactgata ctggcgcaca caaataaacc
    42541 acgatcccgt tattttgcat gaagtgacga ttaaattccg tcagaataat gccgccgcct
    42601 gtagcgataa cggtggatgc cgcagttacc gcttccagcg ccgccgtttc tctggcgcga
    42661 aatcccgccc actcttccct ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc
    42721 aaccactgat cggtatcgac aaaccgacgg ttaagcgaat cggcaagggc cattccgacc
    42781 gttgttttac cacagccccg aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt
    42841 ccccaatagg tcgcaatgcg gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa
    42901 actagtacaa tttcgattgt aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc
    42961 ccttactata ccaataaata ttcaagaatg aagtgtaaat aataaattac atttagccac
    43021 gactacgttg cacttccagc caccacttct ccagctccgc ggcaaaggcc tggcggtcac
    43081 gttgtgaaag gctatctggt ccgccggtct ggatcccact ggcacgtaag gtatccataa
    43141 aatcgcgcat cgtcaggcgc tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat
    43201 taagcgccgc tgcgcctttc tcgatggctt cagcagccaa aggtatatct gcggtgatca
    43261 ccaaatcgcc cgcttcacac tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg
    43321 cgacgcgcag cgtacgaata aatcgcgatg gcggcacgcg taaactctgg tttgctacca
    43381 gtaccagcgg catctgcata cgttccgccg cgcgatacaa aatctcttta attacattgg
    43441 gacacgcgtc ggcatccacc caaattgtca taaagtcatc ctttgttagg taatcctcta
    43501 ttgtgtcgcg cttttgcctt ccggcatagt tctgtttatg cttctgccag cgattatcaa
    43561 aacaatgaat ttcacggcag gagtgaggca atggaaaaga aaatcggttt tattggctgc
    43621 ggcaatatgg gaaaagccat tctcggcggt ctgattgcca gcggtcaggt gcttccaggg
    43681 caaatctggg tatacacccc ctccccggat aaagtcgccg ccctgcatga ccagttcggc
    43741 atcaacgccg cagaatcggc gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc
    43801 gttaaacctg gcatcatgat taaagtgctt agcgaaatca cctccagcct gaataaagac
    43861 tctctggtcg tttctattgc tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc
    43921 catgaccgga aaattatccg cgccatgccg aacactcccg cgctggttaa tgccgggatg
    43981 acctccgtaa cgccaaacgc gctggtaacc ccagaagata ccgctgatgt gctgaatatt
    44041 ttccgctgct ttggcgaagc ggaagtaatt gctgagccga tgatccaccc ggtggtcggt
    44101 gtgagcggtt cttcgccagc ctacgtattt atgtttatcg aagcgatggc cgacgccgcc
    44161 gtgctgggcg ggatgccacg cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt
    44221 tccgcaaaaa tggtgctgga aacgggagaa catccggggg cactgaaaga tatggtctgc
    44281 tcaccgggag gcaccaccat tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc
    44341 gcagtgatcg aagcgatgac gaagtgtatg gaaaaatcag aaaaactcag caaatcctga
    44401 tgactttcgc cggacgtcag gccgccactt cggtgcggtt acgtccggct ttctttgctt
    44461 tgtaaagcgc caaatctgcc gatttcaacc actcacgata gtgactcatt tgtgggttca
    44521 gcggcgcaac ccccacacta atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg
    44581 tgtttagccc ttcacgcacc cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg
    44641 acatgattac tgcaaactca tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg
    44701 taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca tgaccccag
//
LOCUS       DAJTYM010000023        42771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTYM010000023 DAJTYM010000000
VERSION     DAJTYM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(79..615)
                     /gene="ssb1"
                     /locus_tag="OO575_001289"
     CDS             complement(79..615)
                     /gene="ssb1"
                     /locus_tag="OO575_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6664322.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            869..3691
                     /gene="uvrA"
                     /locus_tag="OO575_001290"
     CDS             869..3691
                     /gene="uvrA"
                     /locus_tag="OO575_001290"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6664323.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3726..4082)
                     /locus_tag="OO575_001291"
     CDS             complement(3726..4082)
                     /locus_tag="OO575_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6664324.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4086..4502)
                     /locus_tag="OO575_001292"
     CDS             complement(4086..4502)
                     /locus_tag="OO575_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6664325.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4613..5326)
                     /gene="aphA"
                     /locus_tag="OO575_001293"
     CDS             complement(4613..5326)
                     /gene="aphA"
                     /locus_tag="OO575_001293"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6664326.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5728..5932
                     /locus_tag="OO575_001294"
                     /pseudo
     CDS             5728..5932
                     /locus_tag="OO575_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6435..7628)
                     /gene="tyrB"
                     /locus_tag="OO575_001295"
     CDS             complement(6435..7628)
                     /gene="tyrB"
                     /locus_tag="OO575_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6664327.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7881..8960)
                     /gene="alr"
                     /locus_tag="OO575_001296"
     CDS             complement(7881..8960)
                     /gene="alr"
                     /locus_tag="OO575_001296"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6664328.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9013..10428)
                     /gene="dnaB"
                     /locus_tag="OO575_001297"
     CDS             complement(9013..10428)
                     /gene="dnaB"
                     /locus_tag="OO575_001297"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6664329.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10511..11494
                     /locus_tag="OO575_001298"
     CDS             10511..11494
                     /locus_tag="OO575_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6664330.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11660..11902)
                     /gene="pspG"
                     /locus_tag="OO575_001299"
     CDS             complement(11660..11902)
                     /gene="pspG"
                     /locus_tag="OO575_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6664331.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12036..13073)
                     /gene="dusA"
                     /locus_tag="OO575_001300"
     CDS             complement(12036..13073)
                     /gene="dusA"
                     /locus_tag="OO575_001300"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6664332.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13436..14421)
                     /locus_tag="OO575_001301"
                     /pseudo
     CDS             complement(13436..14421)
                     /locus_tag="OO575_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14739..15254
                     /gene="zur"
                     /locus_tag="OO575_001302"
     CDS             14739..15254
                     /gene="zur"
                     /locus_tag="OO575_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6664333.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15296..15505)
                     /locus_tag="OO575_001303"
     CDS             complement(15296..15505)
                     /locus_tag="OO575_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6664334.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15621..16946)
                     /gene="dinF"
                     /locus_tag="OO575_001304"
     CDS             complement(15621..16946)
                     /gene="dinF"
                     /locus_tag="OO575_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6664335.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17019..17627)
                     /gene="lexA"
                     /locus_tag="OO575_001305"
     CDS             complement(17019..17627)
                     /gene="lexA"
                     /locus_tag="OO575_001305"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6664336.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17737..18105)
                     /gene="dgkA"
                     /locus_tag="OO575_001306"
     CDS             complement(17737..18105)
                     /gene="dgkA"
                     /locus_tag="OO575_001306"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6664337.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18276..20699
                     /gene="plsB"
                     /locus_tag="OO575_001307"
     CDS             18276..20699
                     /gene="plsB"
                     /locus_tag="OO575_001307"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6664338.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20854..21726)
                     /gene="ubiA"
                     /locus_tag="OO575_001308"
     CDS             complement(20854..21726)
                     /gene="ubiA"
                     /locus_tag="OO575_001308"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6664339.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21739..22236)
                     /gene="ubiC"
                     /locus_tag="OO575_001309"
     CDS             complement(21739..22236)
                     /gene="ubiC"
                     /locus_tag="OO575_001309"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6664340.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22459..24039)
                     /locus_tag="OO575_001310"
                     /pseudo
     CDS             complement(22459..24039)
                     /locus_tag="OO575_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24268..25188)
                     /gene="malM"
                     /locus_tag="OO575_001311"
     CDS             complement(24268..25188)
                     /gene="malM"
                     /locus_tag="OO575_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6664341.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25340..26680)
                     /gene="lamB"
                     /locus_tag="OO575_001312"
     CDS             complement(25340..26680)
                     /gene="lamB"
                     /locus_tag="OO575_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6664342.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26752..27867)
                     /gene="malK"
                     /locus_tag="OO575_001313"
     CDS             complement(26752..27867)
                     /gene="malK"
                     /locus_tag="OO575_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6664343.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28232..29422
                     /gene="malE"
                     /locus_tag="OO575_001314"
     CDS             28232..29422
                     /gene="malE"
                     /locus_tag="OO575_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6664344.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29576..31120
                     /gene="malF"
                     /locus_tag="OO575_001315"
     CDS             29576..31120
                     /gene="malF"
                     /locus_tag="OO575_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6664345.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31135..32025
                     /gene="malG"
                     /locus_tag="OO575_001316"
     CDS             31135..32025
                     /gene="malG"
                     /locus_tag="OO575_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6664346.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32396..33871
                     /gene="xylE"
                     /locus_tag="OO575_001317"
     CDS             32396..33871
                     /gene="xylE"
                     /locus_tag="OO575_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6664347.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33915..34325)
                     /gene="psiE"
                     /locus_tag="OO575_001318"
     CDS             complement(33915..34325)
                     /gene="psiE"
                     /locus_tag="OO575_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6664348.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34451..34594)
                     /locus_tag="OO575_001319"
     CDS             complement(34451..34594)
                     /locus_tag="OO575_001319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664349.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34595..34873
                     /locus_tag="OO575_001320"
     CDS             34595..34873
                     /locus_tag="OO575_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664350.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34920..37016)
                     /locus_tag="OO575_001321"
     CDS             complement(34920..37016)
                     /locus_tag="OO575_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6664351.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37016..37753)
                     /locus_tag="OO575_001322"
     CDS             complement(37016..37753)
                     /locus_tag="OO575_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6664352.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37750..38388)
                     /locus_tag="OO575_001323"
     CDS             complement(37750..38388)
                     /locus_tag="OO575_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6664353.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38502..38744)
                     /locus_tag="OO575_001324"
     CDS             complement(38502..38744)
                     /locus_tag="OO575_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664354.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39207..40856)
                     /gene="pgi"
                     /locus_tag="OO575_001325"
     CDS             complement(39207..40856)
                     /gene="pgi"
                     /locus_tag="OO575_001325"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6664355.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41381..42730
                     /gene="lysC"
                     /locus_tag="OO575_001326"
     CDS             41381..42730
                     /gene="lysC"
                     /locus_tag="OO575_001326"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6664356.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10640 a        11008 c        10502 g        10621 t
ORIGIN      
        1 ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa caatataacc
       61 tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg gcggctcgtt
      121 agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc tgaactgatt
      181 gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac caccgatatt
      241 gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg tgccgccaac
      301 gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc atttacgggt
      361 acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc tcgccacttc
      421 tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca tctcgccggt
      481 cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg cgccaccatt
      541 tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa ccttgtttac
      601 gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag tgtaaacgcg
      661 cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc gggaaacaaa
      721 cctggccaga cattgttaca caacactccg gataatgcat tccaatactg tatattcatt
      781 caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat gccaccgggc
      841 aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg gcgcccgcac
      901 ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg tcgtgaccgg
      961 gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg aagggcagcg
     1021 ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga tggaaaagcc
     1081 ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga aatcgacgtc
     1141 tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt tgcgtttgtt
     1201 gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg cggcgcaaac
     1261 cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc tgatgctgct
     1321 cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga acctggcaag
     1381 ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg atccgccgaa
     1441 actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca aggtgcgtga
     1501 cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt ccggtggtac
     1561 agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct ccgccaactt
     1621 cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt tttcgtttaa
     1681 caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat atttcgatcc
     1741 tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc gtggctggga
     1801 tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact ataagttcga
     1861 cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt tgtacggttc
     1921 tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct ccatccgtcg
     1981 tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga cagaatccag
     2041 tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca gctgcgaagg
     2101 gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc tgcctgctat
     2161 ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac tcgctggtca
     2221 gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga aattcctcgt
     2281 aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt ccggcggtga
     2341 agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg ttatgtacgt
     2401 gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt tgggtacgct
     2461 tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg aagacgcaat
     2521 tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg gcggtgaagt
     2581 ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga ccgggcagta
     2641 catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga atccagaaaa
     2701 agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc tcacgctgcc
     2761 agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga cgctgattaa
     2821 cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg ccgaaccggc
     2881 accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata tcgaccaaag
     2941 cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt ttacgcctgt
     3001 gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc caggacgttt
     3061 cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg tgatcaaagt
     3121 ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag gtaaacgcta
     3181 taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag tgctggatat
     3241 gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc gtaagctgca
     3301 aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa ccacactttc
     3361 tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg gcaccgggca
     3421 gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata ttcagcaact
     3481 cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga ttgagcacaa
     3541 tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag gcggcagtgg
     3601 cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg aagcttcgca
     3661 tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg agcggccttt
     3721 tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt cactgcctgc
     3781 tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc gtcgagatac
     3841 acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct gtgctgctga
     3901 cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg attttctact
     3961 tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt ccagtcgtta
     4021 tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc cgaaatggtc
     4081 attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg gatgcgatgt
     4141 tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg taccggcaat
     4201 acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc tgctccctca
     4261 tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca ctccatgtcg
     4321 tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt atgttgcagc
     4381 aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt cagaatttca
     4441 tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt ttgataccac
     4501 atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc gaaaggataa
     4561 aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc tgtcagtatt
     4621 ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt ttgtaggtag
     4681 agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg gcggtaatat
     4741 cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat tgcgattttg
     4801 tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg gcaggaatat
     4861 gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta cgaccagtca
     4921 caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga gcgacctctt
     4981 ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca ggatttttca
     5041 gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc ggactggaaa
     5101 aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct gcgaggctat
     5161 tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc ctggcaacgt
     5221 tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa ctgtttagag
     5281 cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt ttccctaaat
     5341 agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct gaccataacg
     5401 ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc aggatgatta
     5461 tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt attactcgat
     5521 gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga gcgaaatttg
     5581 atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt acaaaaacaa
     5641 tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat ggtgattaat
     5701 tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag caacaaaacg
     5761 acatctttac ctgttgagct agatgcacta aataataaag atatctctta tgccaaagat
     5821 ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag acctggagaa
     5881 gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt agttaacaaa
     5941 atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc cgaggaggtt
     6001 aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca atataaggtt
     6061 aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac gatactataa
     6121 ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat atagaaatat
     6181 tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat gtgcagaaat
     6241 tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata aacttaacaa
     6301 taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca aaaaaatcat
     6361 ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg gtaactccag
     6421 cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg tacatttgcc
     6481 gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc aaattcttca
     6541 cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc gcgctgatta
     6601 agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac caattcctga
     6661 cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct ggctttcaat
     6721 gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg gctggagtag
     6781 ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc ggcatcttca
     6841 cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt cgagaacgaa
     6901 ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta ggcatcctct
     6961 tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag ctcgcgggct
     7021 ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc cgttgggttg
     7081 tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag cgtcgccaac
     7141 aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt actcacttcg
     7201 aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct gacccagacg
     7261 cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc ccctgagccg
     7321 ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc cgcaccaaac
     7381 agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa ataaagcgaa
     7441 gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt caattgtgga
     7501 ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc gctgcgaggg
     7561 tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc gtcaactttt
     7621 tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa taaaccggaa
     7681 gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag agtgagacta
     7741 atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag aacatcaact
     7801 tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc cggcacagac
     7861 aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga agtcaggcgc
     7921 gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac gggcaaacct
     7981 tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc taagtctacg
     8041 cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt caccagcact
     8101 ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc gactacgcca
     8161 agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc tccggcttta
     8221 tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca gccaaaatcg
     8281 gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc cgggcgcacc
     8341 cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat ggaacgttga
     8401 ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt tgcgccacat
     8461 tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg aacgtttttg
     8521 cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc cagacggtgc
     8581 ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc ctcttccagc
     8641 gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga aatcgtcggc
     8701 agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat tccccccgca
     8761 cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc ggggagcgtt
     8821 cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc aaccatttta
     8881 ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg gcggttaatc
     8941 acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat tacctaattc
     9001 ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga agcgcgacca
     9061 ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt taccgataat
     9121 aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac gatagataaa
     9181 catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg agttgaccgg
     9241 acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca ccaccggcac
     9301 gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt ccagcgtacg
     9361 gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga taagcccgat
     9421 gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg gcgtcaggcc
     9481 ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg tgccggaaat
     9541 gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt caacgcgcga
     9601 cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg agaagataag
     9661 tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa atgttgtttt
     9721 acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc cagcggtttt
     9781 tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg gctgctgaaa
     9841 caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct tcggcccttc
     9901 gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa ggtccagcag
     9961 atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat tcgcaaccga
    10021 gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac tgatgttcgc
    10081 cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga cgctatcgag
    10141 ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac taccgctttc
    10201 ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa aatcgtctgc
    10261 taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac cgcccaacac
    10321 cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt gtggatcgcg
    10381 ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag tgaatggagt
    10441 taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga cgatacgttc
    10501 tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg aagtacttca
    10561 agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg aaaataaagc
    10621 catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc cgccatcgct
    10681 acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca gtggtgtaaa
    10741 gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg cttacagctc
    10801 tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt cttttgagca
    10861 agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca aaacctatga
    10921 aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg gcttaattgc
    10981 ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa ccgcgcaaaa
    11041 agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg aagagaatct
    11101 ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt acgattccgt
    11161 gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct taatggtcag
    11221 ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca atcaaaaagg
    11281 ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg aggaattaac
    11341 cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg tcgatgtcgc
    11401 cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc tggaaagccg
    11461 ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc acctgggaag
    11521 ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct gttagaggcg
    11581 caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa ccttaggttg
    11641 tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat ttcggcattt
    11701 ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt aaccacggca
    11761 gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc gccacaacca
    11821 gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca aaaaagccaa
    11881 tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg ctggcattac
    11941 aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg acggtgcgca
    12001 gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc caccagtttg
    12061 agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc acttaaataa
    12121 cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg ccggataata
    12181 tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat ggcacgcact
    12241 actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc taccgccgcc
    12301 agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat atgttgcaaa
    12361 tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt cagatgcggg
    12421 aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc acggttttct
    12481 ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat ctcacactcg
    12541 cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc ctggtcgtcg
    12601 atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat cgctttcacg
    12661 cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc gttctgcacc
    12721 cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc ttctgccagc
    12781 tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc taccggatgt
    12841 tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc tgtggtcacc
    12901 atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg gcaatgtctg
    12961 tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac gtcagttttt
    13021 tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg cattttcaaa
    13081 tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca ctgttcagga
    13141 ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat tgcaaaagaa
    13201 aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat cttattagcc
    13261 gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata aaaaatcaca
    13321 cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa aagaacccaa
    13381 taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc acatattatt
    13441 tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta agtattttaa
    13501 tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt ggtgcagcat
    13561 ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga tgtgacatct
    13621 tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc atctctccca
    13681 ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg tagctctcat
    13741 tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa tatgaaagtg
    13801 aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg caggcattaa
    13861 caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa ggttcattat
    13921 ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta gtttctacat
    13981 caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta catatactat
    14041 taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca cgatcgttta
    14101 aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga tcatttatgt
    14161 gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga ttcgttgaat
    14221 aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat aatcaataga
    14281 tttaccatat atattattta atttaccttt gattgaacct ctattatcca atttatcttt
    14341 ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt atccagttgc
    14401 accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg tcctccttat
    14461 aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac tacaggtatg
    14521 cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt aaaaatgaat
    14581 tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct tacctacaaa
    14641 acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg gaacatggta
    14701 aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc acaacgcagg
    14761 agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg accccacagc
    14821 gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct tatgatctgc
    14881 ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat cgcgcgctgg
    14941 attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt tatgtgctct
    15001 gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat cgctgcggcg
    15061 cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg gcggcaaaaa
    15121 tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg gcatgcgtgg
    15181 aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg caggtgaaaa
    15241 agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg attaattacc
    15301 agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga tctttctgat
    15361 aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca atgatcgtca
    15421 tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac tgtttccagt
    15481 taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt gaatgagagt
    15541 acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc ttaggataat
    15601 ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg ccagtgacgc
    15661 cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa tgccagccac
    15721 aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc tgcggcggcc
    15781 accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat gccgtccagc
    15841 agataacacc agacgccaac caacggcaaa atcacctgcc agataagata gcggtcagcc
    15901 agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc tgccagcaaa
    15961 taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc ccgccataca
    16021 tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc ttcaaccgcg
    16081 taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag aaccgcgtta
    16141 acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc gaaacagagt
    16201 tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg acggaagttt
    16261 cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag gattttacgc
    16321 accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc cagcgccgcg
    16381 ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat gttgaggata
    16441 ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag taaccaaccg
    16501 agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc aagaaagcgc
    16561 cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag atcgataatc
    16621 ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa cggttgcacc
    16681 agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt cagcccggtg
    16741 gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt tgcaccaacc
    16801 gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt atcgaccagt
    16861 cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg ccagagtgct
    16921 ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga tgaaaaacaa
    16981 accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc gccgttgcga
    17041 ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc aacgacaatt
    17101 ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt tttcaggcgc
    17161 ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg tacatcctga
    17221 gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat cgacatcccg
    17281 ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac ctgataatga
    17341 ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc taccagcggc
    17401 aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga aacaatttca
    17461 ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg ggaacggaac
    17521 cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct gatgtgatca
    17581 cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc gccccctggg
    17641 tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa ccataaactg
    17701 cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg accataacag
    17761 gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg atcccatatc
    17821 ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca ctgcttcgat
    17881 ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa gcagcacgcg
    17941 ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata ccgctacgcc
    18001 ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc aggaatagcc
    18061 agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa gaaccttttt
    18121 acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt tgcgtaacgc
    18181 cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat ccttgccgcg
    18241 catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca cgaatttact
    18301 acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt ccggcagatc
    18361 ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta ccgtacaact
    18421 cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg cctgacccgt
    18481 tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt cacggcgggc
    18541 cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc cacgactatc
    18601 tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta tcggtgatgt
    18661 ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt atgcttaacg
    18721 gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg cgtttctcgc
    18781 cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact atcgctcaga
    18841 aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc gtaggcccac
    18901 gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc attgccaaag
    18961 cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag cagaacgcga
    19021 ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc ctgactgacc
    19081 gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat aacgccgagc
    19141 gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc caccgcagtc
    19201 acatggacta cctgctactt tcttacgtgc tgtaccacca gggactggtg ccgccgcata
    19261 tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc cgtctggggg
    19321 cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt ttccgtgagt
    19381 atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa ggcggtcgtt
    19441 cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc attcaggcga
    19501 tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt tatgagcacg
    19561 tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag aaagagagcc
    19621 tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt tacgtcaact
    19681 tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac tggcgtgaat
    19741 ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc aataatattg
    19801 ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg aacctgtgct
    19861 gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta accgagcaac
    19921 tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct accgttcctt
    19981 cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt gaagtcgaga
    20041 aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg atgacctact
    20101 atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca attgtcaccc
    20161 agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt tacccaatgc
    20221 tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt attgatgcgc
    20281 tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag ttgcatatca
    20341 acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa acgctgcaac
    20401 gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc ggtacgctgg
    20461 agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc aacgcgccgg
    20521 agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat gaagggtata
    20581 tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag ttgctggcgg
    20641 agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc gaagggtaat
    20701 cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga cctgcaaatt
    20761 caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc aacccagaag
    20821 aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac tcattgccag
    20881 tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa agcaggcttc
    20941 acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag ccaccacaat
    21001 tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca tcagtgccag
    21061 tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca ggattgccgt
    21121 ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact gcgtgtcgta
    21181 agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg gcaccgactc
    21241 actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca gcaccacttg
    21301 cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca gagcggcaat
    21361 cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa tcagtaccag
    21421 cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa gtggtcggtt
    21481 cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca ccacgcaacc
    21541 agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga gctgaggaac
    21601 gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta acgcgccaat
    21661 tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg tcagactcca
    21721 ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt ctgttagcaa
    21781 cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg catcacggcc
    21841 tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc ctaacggcgt
    21901 tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag gaacgacggt
    21961 acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta accagtaacg
    22021 agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga caaacccctc
    22081 gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg tcatggaatc
    22141 ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt taaaatagcg
    22201 cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt tacataacgt
    22261 aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta agtgatctaa
    22321 tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac cttacgcttt
    22381 gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga tgctaggaac
    22441 ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc gccaactcgc
    22501 agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa gtgtttcctg
    22561 agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg aattccaatt
    22621 ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca ccataacagg
    22681 cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta atttattagt
    22741 gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat catcactacg
    22801 aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg ttgatcgtaa
    22861 ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc ctttattcat
    22921 attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct tactgaatcg
    22981 atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat aactacgtat
    23041 aattggatct tttatataag gacaatggct aaatgttttc ttcatattct caataaagtt
    23101 tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca gaacggattt
    23161 caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat tgatataacc
    23221 atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca attgttctgt
    23281 aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa cagctgtcaa
    23341 attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg tgaagtgcga
    23401 agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc gaaaattact
    23461 atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc taaaatcaac
    23521 acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt taaatatgac
    23581 attatcaact gttgccttag taaaattgca attctcaatt tctttacatt cgaaagttac
    23641 atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt taaaatgaca
    23701 atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat ccatcagaaa
    23761 attacaagcg cattctattt tttcatttct gtagaatttt acacttatag tatcattact
    23821 aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt gttttatatt
    23881 taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca ccccattgaa
    23941 attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat gtttcatcaa
    24001 agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt aatacagaaa
    24061 taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag gaaaagaatc
    24121 tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag tactataaaa
    24181 aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac gcaccagttg
    24241 ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc tgataaaggt
    24301 ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta acgccttatc
    24361 aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt ccgtgtcgtt
    24421 gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag ctggtgccgc
    24481 cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc ctaccaacac
    24541 gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg tatgacgagc
    24601 aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg ctttagccgg
    24661 gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca gaacataaag
    24721 tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat ctgcactcat
    24781 cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg gggtcatgtt
    24841 ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca cttcgctggt
    24901 cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa ccggaccact
    24961 gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct gggttttaga
    25021 ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg ctggcgcgtc
    25081 gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg cgcttgccag
    25141 taaccctgct gataaacaga gggcgatgag acttttattc attttcattg ttttcacctc
    25201 aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa tcagaggggc
    25261 tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt aaacgcctta
    25321 tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag gtccactcgt
    25381 cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct ttgccgtagt
    25441 tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc cacttggcgt
    25501 aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc tgccattgtt
    25561 gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat tcgacgttgt
    25621 cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc gggcgaatac
    25681 caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg tacataccca
    25741 cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg atacgcagca
    25801 tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg acgccagaac
    25861 cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga acaacaaact
    25921 tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag ccgtctttcg
    25981 atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag tcgacaccca
    26041 gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg aaaacgtcgt
    26101 tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa gaaccaccag
    26161 cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg atgttttcca
    26221 gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga acgtcatgac
    26281 gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg atcaggtttt
    26341 ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca ttctgttgcg
    26401 cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct ttccacactt
    26461 cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg tatttacttt
    26521 gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag ccaataccgg
    26581 aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg cccgctgcga
    26641 cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct gagtcattgc
    26701 ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg cttaaacgcc
    26761 cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat ggcaacgctc
    26821 tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt cgttctggcg
    26881 gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt cgttgccgag
    26941 ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac tcggcagtag
    27001 atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac ggctttcaac
    27061 aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt gatcgattgc
    27121 ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa atccggcgac
    27181 aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg ccacgcgacc
    27241 ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat cgtgggtgac
    27301 gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac gcatttgcac
    27361 acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc ttggctcggc
    27421 caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg ctttcggttt
    27481 gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc gttggttaat
    27541 cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg ctactgacag
    27601 gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg ctggcggagt
    27661 gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct caagcccggc
    27721 aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca ccacgaattc
    27781 accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc aggctttcgt
    27841 tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc tgtcatcgac
    27901 agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat cctccacccc
    27961 ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt gaactaaacc
    28021 gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt ttatgtgcgc
    28081 atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt ggggcgtagg
    28141 ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt aggtgttttc
    28201 acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg cacgcatcct
    28261 cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca aaatcgaaga
    28321 aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg
    28381 taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga
    28441 agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct tctgggcgca
    28501 cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc
    28561 gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg gcaagctgat
    28621 tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc tgctgccgaa
    28681 cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa
    28741 gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga
    28801 cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga
    28861 taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat
    28921 gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat
    28981 gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt
    29041 aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg tgctgagcac
    29101 cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg aaaactatct
    29161 gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct
    29221 gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca tggaaaacgc
    29281 ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtacg
    29341 tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc
    29401 gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa cgccttgtcc
    29461 ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg catcaggcag
    29521 ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga accccatgga
    29581 tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag tgctaggtct
    29641 gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg aatacctgtt
    29701 cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca atcgtaaagc
    29761 ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg tcctcttccc
    29821 tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc agctgacttt
    29881 tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa cctataactt
    29941 tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg aaaccggcaa
    30001 aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac tgaaagaaac
    30061 gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga atcgtcaggc
    30121 gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga gctccctgcg
    30181 ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt tgacgaataa
    30241 tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt ccattaccgc
    30301 cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca ccggctggaa
    30361 aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg ccattttcgt
    30421 ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg gcatggttct
    30481 ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg tcctgctgat
    30541 tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat tgtttaacca
    30601 gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc ccgcctggtt
    30661 cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc tgggttatcc
    30721 gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt tgtatgaagc
    30781 ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc tgccgctgct
    30841 gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta acaacttcgt
    30901 gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca cgccagccgg
    30961 ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg gcgggggtca
    31021 ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg gtgcgctggc
    31081 gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac aaaaatggca
    31141 atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct actgctactt
    31201 tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct gcgtcaggga
    31261 aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg gaaactggcg
    31321 ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt cccggtactg
    31381 ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat tgtggcgctc
    31441 tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc gacgctgctg
    31501 aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc gttgtatgcg
    31561 ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca cggcggcgta
    31621 attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg ctatttcgaa
    31681 accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc gtggcaggcc
    31741 ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt catcctgtcg
    31801 tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga cgtaaacagc
    31861 tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct gtggggtgac
    31921 tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt gctggctcaa
    31981 cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg ttgttctgcc
    32041 aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat tattcggcgc
    32101 tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg ttaatattcc
    32161 catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa ttctctttcg
    32221 tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc acaattaaga
    32281 tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt aactgcctga
    32341 caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg tctgaatgaa
    32401 tacccagtat aattccagtt atatattttc gattacctta gtcgctacat taggtggttt
    32461 attatttggc tacgacaccg ccgttatttc cggtactgtt gagtcactca ataccgtctt
    32521 tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat tttgcgtagc
    32581 cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca gtaaccgctt
    32641 cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg gtgtaggttc
    32701 tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac ctatttatct
    32761 ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg gcgttggttt
    32821 agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata ttcgcggaaa
    32881 actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt actgcgtaaa
    32941 ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca ggcgttatat
    33001 gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata ccgtgccaga
    33061 aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta tcctgcgcaa
    33121 aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact ccctggatca
    33181 tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg taatcggcgt
    33241 tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact acgcgccgga
    33301 agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca ttattgtcgg
    33361 agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat ttggtcgtaa
    33421 gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc tcggtaccgc
    33481 gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct atgttgccgc
    33541 ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct tcccgaatgc
    33601 tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga actacttcgt
    33661 ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt tccacaacgg
    33721 tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta tgtggaaatt
    33781 tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg aaccggaaac
    33841 gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc gcgccatttc
    33901 ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca cagggtgatc
    33961 accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt atgatcgacg
    34021 atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag cgggaagtga
    34081 aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag aaagtaaacc
    34141 accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag cacatcagcc
    34201 agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca cagcaggcca
    34261 agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg agagagtgac
    34321 gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa atgtgacgga
    34381 gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc tccaccaaag
    34441 ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc ggcaagacaa
    34501 accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag ccaccagcaa
    34561 tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga taaaggtggt
    34621 aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc ctgcttatgc
    34681 tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct ccagcccaat
    34741 gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca ggaagacaga
    34801 atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc ctgatggctc
    34861 aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt tcccatgact
    34921 tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt acgcccaagt
    34981 tgctgaccac cgtcacgcgt cagcggcgtc cagccaatcg ccgcacggct gcgtgtcggg
    35041 ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa gtccccttcg
    35101 ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac cacgccgctg
    35161 tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata ctgcccgacg
    35221 ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt cagatgtccg
    35281 gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca gtcgcgctgt
    35341 ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg atacaacact
    35401 tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg accgtagaag
    35461 ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac atcgttctgc
    35521 acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg tggattggtg
    35581 tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt ggtcagtaga
    35641 tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat gtaaaagttt
    35701 tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa gcgtgatttg
    35761 tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg tttgtgcgcc
    35821 agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct ggcgacatcg
    35881 gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat cgttttgatc
    35941 ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc gcgcgaatca
    36001 cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg gatctgcgga
    36061 tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac cgaatgctgc
    36121 aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat gtaggacggg
    36181 cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt gccacgttca
    36241 tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac gttaaactta
    36301 cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc atactccagt
    36361 ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag tgaggcagga
    36421 ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt atcgcgatag
    36481 caatatttat cactggctga acagagcgga ttttttacat taccgctggt gcccagatat
    36541 ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc aacaagatat
    36601 tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac ttgcggcagc
    36661 cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt atacgtttga
    36721 tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt gtagcgcagc
    36781 gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta ctgatcgtta
    36841 tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg agtttgtaat
    36901 aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata tgtttcgccg
    36961 tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt tttcattatt
    37021 ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca ggcgtctcac
    37081 tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc tctacgtgac
    37141 gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa acgacccacg
    37201 cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc gccacgtcgc
    37261 gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac gtgaccgtgg
    37321 acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta ccgcgttcgg
    37381 caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact ttcaatgcct
    37441 ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa tctgcgccct
    37501 gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc gttgccagct
    37561 cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc cctaccagat
    37621 cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg ctgaacacct
    37681 tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac gcgacaatag
    37741 tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga aataatccgc
    37801 gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt agcggttatg
    37861 ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg tttcatcgct
    37921 gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg tggcgtagcg
    37981 tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat caacaatttg
    38041 tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt tgtcgccgcc
    38101 aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat cctgagtgac
    38161 ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg gcccgccatt
    38221 aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg tcagctgtac
    38281 gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag tcgtggctga
    38341 acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga ctcttctcca
    38401 gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata cttcaagcca
    38461 cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt actggtggtc
    38521 gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc gagcgcgaca
    38581 cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc cgaaaccgac
    38641 gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc agaagtcgcc
    38701 gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat ttcccttcat
    38761 tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac acaactaaag
    38821 catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg aatgaatatc
    38881 aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta aaataaaaaa
    38941 caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc tattttagga
    39001 ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct gataaaacaa
    39061 tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact atgcattaat
    39121 gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta tccggcctac
    39181 atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg gttaatcaga
    39241 ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc tggcagaata
    39301 cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa gatgttcagg
    39361 atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc caggctgaac
    39421 ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa cactttgaac
    39481 ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc ctgctcaacc
    39541 acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt agacagcagt
    39601 ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa atcgcacggt
    39661 accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt agtgcctggt
    39721 tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg gtcaacatac
    39781 ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg catatactgg
    39841 tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca gatgccaatc
    39901 agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg cttgtccatt
    39961 gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag aacaatcgac
    40021 aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa ctcgaacatg
    40081 ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag cgccgcaaaa
    40141 tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg cgcgctatgg
    40201 gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa cagcgtggtt
    40261 tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt agaaacaaag
    40321 tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta cgggccgagg
    40381 tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt ataacccttc
    40441 cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac cgcgttgact
    40501 tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt acgcagcgct
    40561 acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa catcgactta
    40621 atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag cgtctcttca
    40681 gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc ggagaactta
    40741 gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt catttcatcg
    40801 aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt tttcattagc
    40861 aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag tcacagaaaa
    40921 atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc gcagtgaagc
    40981 atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg accccgtcgt
    41041 tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag cgcaggccag
    41101 aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata acacctgagg
    41161 gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta caggggctga
    41221 atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg gtggagcact
    41281 tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg ctcttccctt
    41341 gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa ttgttgtctc
    41401 caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg ctgatattgt
    41461 gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta tcactaatct
    41521 gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac tcgacgctat
    41581 tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta tccgtgaaga
    41641 gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc tggcaacgtc
    41701 tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc tgctgtttgt
    41761 cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta aagtgatgcg
    41821 taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg aactggccgc
    41881 gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat ttatcggtag
    41941 cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata cggcagcctt
    42001 gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc cgggcatcta
    42061 caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg cgtttgccga
    42121 agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt tgctacccgc
    42181 agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg caggtggtac
    42241 gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc ttcgtcgcaa
    42301 tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct tcctcgcgga
    42361 agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca cgtcagaagt
    42421 gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata cgttgctgac
    42481 gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag aaggtctggc
    42541 gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca aagaggtatt
    42601 cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca gccataacct
    42661 gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc atagtaattt
    42721 gtttgagtaa atactgtatg gcctggaagc tatatttcgg gccgtattga t
//
LOCUS       DAJTYM010000024        42663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTYM010000024 DAJTYM010000000
VERSION     DAJTYM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1117
                     /gene="cscA"
                     /locus_tag="OO575_001327"
     CDS             <1..1117
                     /gene="cscA"
                     /locus_tag="OO575_001327"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6664357.1"
                     /translation="GVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVIL
                     TPPEGIMHFRDPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADA
                     GESYMWECPDFFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGH
                     FTELDNGHDFYAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNG
                     KLLQRPVHEAESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQL
                     GTGMRLYIDNQSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDG
                     EAVMSSRIYPQPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1125..2183)
                     /locus_tag="OO575_001328"
     CDS             complement(1125..2183)
                     /locus_tag="OO575_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6664358.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAYRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2351..2569
                     /locus_tag="OO575_001329"
                     /pseudo
     CDS             2351..2569
                     /locus_tag="OO575_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2587..3915
                     /gene="dsdA"
                     /locus_tag="OO575_001330"
     CDS             2587..3915
                     /gene="dsdA"
                     /locus_tag="OO575_001330"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6664359.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4023..5561)
                     /gene="emrY"
                     /locus_tag="OO575_001331"
     CDS             complement(4023..5561)
                     /gene="emrY"
                     /locus_tag="OO575_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6664360.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5561..6724)
                     /gene="emrK"
                     /locus_tag="OO575_001332"
     CDS             complement(5561..6724)
                     /gene="emrK"
                     /locus_tag="OO575_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6664361.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7140..7754
                     /gene="evgA"
                     /locus_tag="OO575_001333"
     CDS             7140..7754
                     /gene="evgA"
                     /locus_tag="OO575_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6664362.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            7759..11352
                     /gene="evgS"
                     /locus_tag="OO575_001334"
     CDS             7759..11352
                     /gene="evgS"
                     /locus_tag="OO575_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6664363.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11408..12553)
                     /gene="yfdE"
                     /locus_tag="OO575_001335"
     CDS             complement(11408..12553)
                     /gene="yfdE"
                     /locus_tag="OO575_001335"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6664364.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12627..13571)
                     /gene="yfdV"
                     /locus_tag="OO575_001336"
     CDS             complement(12627..13571)
                     /gene="yfdV"
                     /locus_tag="OO575_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6664365.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13641..15335)
                     /gene="oxc"
                     /locus_tag="OO575_001337"
     CDS             complement(13641..15335)
                     /gene="oxc"
                     /locus_tag="OO575_001337"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6664366.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15389..16639)
                     /gene="frc"
                     /locus_tag="OO575_001338"
     CDS             complement(15389..16639)
                     /gene="frc"
                     /locus_tag="OO575_001338"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6664367.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17152..17775)
                     /locus_tag="OO575_001339"
     CDS             complement(17152..17775)
                     /locus_tag="OO575_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6664368.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18072..18347
                     /locus_tag="OO575_001340"
     CDS             18072..18347
                     /locus_tag="OO575_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664369.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18424..18666)
                     /locus_tag="OO575_001341"
     CDS             complement(18424..18666)
                     /locus_tag="OO575_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6664370.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19019..19939
                     /gene="lpxP"
                     /locus_tag="OO575_001342"
     CDS             19019..19939
                     /gene="lpxP"
                     /locus_tag="OO575_001342"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6664371.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20294..20365
                     /gene="ypdK"
                     /locus_tag="OO575_001343"
     CDS             20294..20365
                     /gene="ypdK"
                     /locus_tag="OO575_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6664372.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20430..21668)
                     /gene="alaC"
                     /locus_tag="OO575_001344"
     CDS             complement(20430..21668)
                     /gene="alaC"
                     /locus_tag="OO575_001344"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6664373.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22045..23531
                     /locus_tag="OO575_001345"
                     /pseudo
     CDS             22045..23531
                     /locus_tag="OO575_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            23546..24280
                     /gene="ypdB"
                     /locus_tag="OO575_001346"
     CDS             23546..24280
                     /gene="ypdB"
                     /locus_tag="OO575_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6664374.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24293..25150
                     /locus_tag="OO575_001347"
                     /pseudo
     CDS             24293..25150
                     /locus_tag="OO575_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25153..27648)
                     /gene="ptsP"
                     /locus_tag="OO575_001348"
     CDS             complement(25153..27648)
                     /gene="ptsP"
                     /locus_tag="OO575_001348"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6664375.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(27673..28710)
                     /gene="ypdE"
                     /locus_tag="OO575_001349"
     CDS             complement(27673..28710)
                     /gene="ypdE"
                     /locus_tag="OO575_001349"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6664376.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(28710..29793)
                     /gene="ypdF"
                     /locus_tag="OO575_001350"
                     /pseudo
     CDS             complement(28710..29793)
                     /gene="ypdF"
                     /locus_tag="OO575_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(29808..31055)
                     /locus_tag="OO575_001351"
     CDS             complement(29808..31055)
                     /locus_tag="OO575_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6664377.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31077..31403)
                     /locus_tag="OO575_001352"
     CDS             complement(31077..31403)
                     /locus_tag="OO575_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6664378.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31622..32587)
                     /gene="glk"
                     /locus_tag="OO575_001353"
     CDS             complement(31622..32587)
                     /gene="glk"
                     /locus_tag="OO575_001353"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6664379.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            32791..34047
                     /locus_tag="OO575_001354"
     CDS             32791..34047
                     /locus_tag="OO575_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6664380.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34162..34488
                     /locus_tag="OO575_001355"
     CDS             34162..34488
                     /locus_tag="OO575_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6664381.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34629..35867)
                     /locus_tag="OO575_001356"
     CDS             complement(34629..35867)
                     /locus_tag="OO575_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6664382.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36203..37405
                     /gene="nupC"
                     /locus_tag="OO575_001357"
     CDS             36203..37405
                     /gene="nupC"
                     /locus_tag="OO575_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6664383.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37455..39567)
                     /locus_tag="OO575_001358"
                     /pseudo
     CDS             complement(37455..39567)
                     /locus_tag="OO575_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(39775..39850)
                     /locus_tag="OO575_001359"
     tRNA            complement(39775..39850)
                     /locus_tag="OO575_001359"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39815..39817),aa:Ala,seq:ggc)
     gene            complement(39890..39965)
                     /locus_tag="OO575_001360"
     tRNA            complement(39890..39965)
                     /locus_tag="OO575_001360"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39930..39932),aa:Ala,seq:ggc)
     gene            40186..40545
                     /locus_tag="OO575_001361"
     CDS             40186..40545
                     /locus_tag="OO575_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6664384.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40547..40939
                     /locus_tag="OO575_001362"
     CDS             40547..40939
                     /locus_tag="OO575_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6664385.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(40991..42406)
                     /gene="gltX"
                     /locus_tag="OO575_001363"
     CDS             complement(40991..42406)
                     /gene="gltX"
                     /locus_tag="OO575_001363"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6664386.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT        11348 a        10631 c         9898 g        10786 t
ORIGIN      
        1 tggtgtcctc tcacttatct acaccggaca cgtctggctc gatggtgcag gtaatgacga
       61 tgcaattcgc gaagtacaat gtctggctac cagtcgggat ggtattcatt tcgagaaaca
      121 gggtgtgatc ctcactccac cagaaggaat catgcacttc cgcgatccta aagtgtggcg
      181 tgaagccgac acatggtgga tggtagtcgg ggcgaaagat ccaggcaaca cggggcagat
      241 cctgctttat cgcggcagtt cattgcgtga atgggccttc gatcgcgtac tggcccacgc
      301 tgatgcgggt gaaagctata tgtgggaatg tccggacttt ttcagccttg gcgatcagca
      361 ttatctgatg ttttccccgc agggaatgaa tgccgaggga tacagttacc gaaatcgctt
      421 tcaaagtggc gtaatacccg gaatgtggtc gccaggacga ctttttgcac aatccgggca
      481 ttttactgaa cttgataacg ggcatgactt ttatgcacca caaagctttt tagcgaagga
      541 tggtcggcgt attgttatcg gctggatgga tatgtgggaa tcgccaatgc cctcaaaacg
      601 tgaaggatgg gcaggctgca tgacgctggc gcgcgagcta tcagagagca atggcaaact
      661 tctacaacgc ccggttcacg aagctgagtc gttacgccag cagcatcaat ctgtctctcc
      721 ccgcagaatc agcaataaat atgttttgca ggaaaacgcg caagcagttg agattcagtt
      781 gcagtgggcg ctgaagaaca gtgatgccga acattacgga ttacagctcg gcactggaat
      841 gcggctgtat attgataacc aatctgagcg acttattttg tggcggtatt acccacacga
      901 gaatttagac ggctaccgta gtattcccct cccgcagcgt gacacgctcg ccctaaggat
      961 atttatcgat acatcatccg tggaagtatt tattaacgac ggggaagcgg tgatgagtag
     1021 tcgaatctat ccgcagccag aagaacggga actgtcgctt tatgcctccc acggagtggc
     1081 tgtgctgcaa catggagcac tctggctact gggttaacat aatatcaggt ggaacaacgg
     1141 atcaacagcg ggcaagggat ccgcgtcact cttccccctt cacgaccttc aataatatgc
     1201 aatgcagctt cccgcccgat aatgtcatgt ggaagctgaa ttgtggtcag cggcggtaaa
     1261 aacagatgcc cgacgccaac cagattatca aagcccatta cagcgacatc ctgcgggata
     1321 cgtaccccct tcgccaaaag aacctgataa gccacaaagg ctgcgcgatc gttaccacat
     1381 atcagaacat caaaatctgg tttgcccgat ttgaagtgag cattgagtaa acttgcgaga
     1441 tcggtgtagt gatcatcacc tgttgccatg tgaaattgtt tcacctcagc cagatctcgt
     1501 ccagcatcac gccaagcctg ctcaaatccc tgccgacgat accctgttgc caacgcactt
     1561 tccggtagcc agaagcataa tggttgacga tagcccgccg cgagcaaatg ctgtgttgat
     1621 tcatattgtg cagtgtaatc atcagggata taactgggta acgctgggtc atccgccaca
     1681 cagttcgcca atacaatatt ttcaccatac agagactcag gcagcgtgat atgtcgcagc
     1741 cccattgtag tatagataat gccatccgga cggtaggcaa gcagctgacg tgccgcgcgg
     1801 gcagcgtcat cttcagaaaa aatattgatt aaaaaactat tccagccgaa ctcgctggcg
     1861 gtttgctcaa tggcaagcag aatatcaaca gagaaaggag tggtagccgt gtcctgcgcc
     1921 agcacggcga gagtcgacgg cttacgtcct tgagcgcgca tcttacgggc ggaaagatca
     1981 ggaacataat tcagggtctg gattgcctgc aatacgcggt cacgcgttgc aggacgcaca
     2041 gattctgcat tatgcatcac ccgggagact gtcatcatcg acactcccgc caggcgtgcg
     2101 acatccttta atgaagccat acccaagccg tttgccgtaa aacgggcact gtagcagaaa
     2161 cagacgtcac tggcgagatc caacgcccta tcacctgaca cagcaataca ataaaaaata
     2221 acaataattc ccggacaatt gtccccaatt ccgcctctgt tctcgccaac gagtaaccat
     2281 ataggcaagt aagtaaaaca tgccaactgg catcgccaac ctggagaaga aaatataaat
     2341 atggatcatt gtgctgccac tgtatcccga catcagcccg gaaattattg cgattgctat
     2401 cggttcaggt gcaattggct gcacgatcgt tacggactcg cttttctggc tggtgaagca
     2461 atattgcggc gctacgctca atgaaacatt taaatactat acgacagcga catttatcgc
     2521 ttcagtcgtc gctctggcgg gcacattcct gctgtcattt atcatctaag cgcaaagaga
     2581 cgtactatgg aaaacgctaa aatgaactcg ctcatcgccc agtatccgtt ggtaaaggat
     2641 ctggttgctc ttaaagaaac cacctggttt aatcctagca cgacctcatt ggctgaaggt
     2701 ttaccttatg ttggcctgac cgaacaggat gttcaggacg cccatgcgcg cttatcccgt
     2761 tttgcaccct atctggcaaa agcatttgct gaaacagctg ccactggggg gattattgaa
     2821 tcagaactgg ttgccattcc ggctatgcaa aaacggctgg aaaaggaata tcaacaaccg
     2881 atcagcgggc aactcctact gaaaaaagat agccatttgc ccatttccgg ctctataaaa
     2941 gcacgcggcg ggatttatga agtcctggca catgcagaaa aactggctct ggaagcgggg
     3001 ttgctgacgc ttgatgatga ctacagcaaa ctgctttctc cggagtttaa acagttcttt
     3061 agccaataca gcattgctgt gggctcaacc ggaaatctgg ggttatcaat cggcattatg
     3121 agcgcccgca ttggctttaa ggtgacagtt catatgtctg ctgatgcccg ggcatggaaa
     3181 aaagcgaaac tgcgcagcca tggcgttacg gtcgtggaat atgagcaaga ttatggtgtt
     3241 gccgtcgagg aaggacgtaa agcagcgcag tctgacccga actgtttctt tattgatgac
     3301 gaaaattccc gcacgttgtt ccttgggtat tccgttgctg gccagcgtct taaagcgcaa
     3361 tttgcccagc aaggccgtat cgtcgatgct gataaccctc tgtttgtcta tctgccgtgt
     3421 ggtgttggcg gtggtcctgg tggcgtcgca ttcgggctta aactggcgtt tggcgatcat
     3481 gttcactgct tttttgccga accaacgcac tccccttgta tgttgttagg cgtccataca
     3541 ggattacacg atcagatttc tgttcaggat attggtatcg acaaccttac cgcagcggat
     3601 ggccttgcag ttggtcgcgc atcatgcttt gtcgggcggg caatggagcg tctgctggat
     3661 ggcttctata cccttagcga tcaaaccatg tatgacatgc ttggctggct ggcgcaggaa
     3721 gaaggtattc gtcttgaacc ttcagcactg gcgggtatgg ccggacctca gcgcgtgtgt
     3781 gcatcagtaa gttaccaaca gatgcacggt ttcagcgcag aacaattgcg taatgccact
     3841 catctggtgt gggcgacggg aggtggaatg gtgccggaag aagagatgaa tcaatatctg
     3901 gcaaaaggcc gttaataacg tttcaacgca acatcgcaat cctttccctg ggtgagcgat
     3961 gctgccgatg gcgcagactt aagatccccg gtcttacccg ctataacccc ctccttttat
     4021 catcacccaa cgcctttcgc tgtaaacggc ggtttcgcaa accaaaccaa aaccgtaagt
     4081 aagataaaag caatagccgc catacggaaa atctcatttg cagaaataga aagtgactgc
     4141 tgggttattt cattattaat ttcattaaga actcctgaaa gcgaaccata gtatttatcc
     4201 ataatttgtg acgatgaatt aaacacgggg ttaaattgat cgatggttgc tgtcaactga
     4261 ctatggtgta acgattcgcg tcgtccccac agcgtcattg tcaacgacgt accaactgat
     4321 cctgacaagg tacgaaaaaa attactcata ctcgaggcat tggcaaattt attatctggc
     4381 aagcctgaaa acgaaatcgt tgttaagggt aaaaagaaac aggcaacggc gaatccctgg
     4441 aaaaactgtg gcaaaatgat gcctgtaaaa tcaatcgttg gcataaatgt cacagaacgc
     4501 cagtaatagc aaaccgcata catcaaaaaa ctgaatgtca ctaacacccg catgtctatt
     4561 ttattgccat aacgtcctat caaaggtgaa atcaatagtg gcatgatgcc gatgggcgca
     4621 taagcaagcc cggcccatat cgcattatac cccatcgttt tctggagtaa ctgcggcata
     4681 aggacgatcg ctccagagta aaataaatac gcgcaggtga tactcacaat accaatggtg
     4741 aaattacggg acttaaacaa actgagatca agaatcgggt tctctgaggt cgattcccaa
     4801 atgactaaag agatcagaga aataactgat actactgtta atattattat tgtactcgag
     4861 ttgaaccaat ccagatcgcg ccctttatca agcataattt gcaagccacc aacaccgagc
     4921 actaacaggg tcagtcttgg tagattcatt tttaccggtg aagtttcagt ttctcttcct
     4981 ttaagtaagg ttaagcataa tgtcaggacg ataatcccca tagggacatt gattaaaaat
     5041 atccaacccc agctaaagtt atcgcaaata tagccgccca atatcggccc acatatcgga
     5101 gcgataatca cggtcattga ccataatgcc agagcaaatg ttcttttttc tggtggataa
     5161 ttccttaata ataaactctg tgacagtgga attaacggcc ccgccattaa tccctgaacg
     5221 actctaaaaa atatcagcac atcaagattg gtcgataaac tacacattaa ggaagacagc
     5281 gaaaaaaaag tgactgaaag taaaaataac cttaattcgc ctattctttg tgccagcctg
     5341 ccggtaacag ggatcgcaat ggcatttgct acgccaaacg aggtgataac ccaggtgcct
     5401 tcgtctgttg atgctcccag aaagccagat attgtcggta ttgcgacgtt agaaatagtg
     5461 gaatccaaca tttgcataaa cgtcgctaat gacaatgcaa tagtaacgca ccataacgtc
     5521 ccaccggtta atggtgccgg agttgattta gtgattgcca ttaaagttgt ccattatgcg
     5581 aaataatgtt gctaatttct ttttctatcg gactggtatc gataactaaa gccttactgg
     5641 tataagcagg catggaggtc acggttgaag ccagatcagg catctcagca atgtcttcgt
     5701 ccttcgtatc aatagttgca gtcatcgata aaccaatgcg caaggggtgt tccatgagtt
     5761 cttttggatc aagagaaact tcaaccggta cacgctgaac gattttgatc cagttccctg
     5821 tcgcattttg tgcaggtaat aaggagaacg cattgccggt tcccatattg atccctgtca
     5881 cccgaccatg aaacacaaca ttttcaccat aaagatcgct gataatattg actgattgac
     5941 caatccgtac atccgtgagt tgtgtttctt taaagttggc attaacccac atttgacgtg
     6001 ccggtacgac agccattaac gattgtccgg aactcactgt ttcgccgacc tgaacacttc
     6061 gctgggcaat atagccagta accggactcc ttatatccgt acgtttaagc accaaccagg
     6121 cttctttggt tgcatccgcc gcttcaacta cttgtggctg acggtttaat ggtgtgttca
     6181 ttactaaagc tttattcgct ttataagcct ggatagcggc attcagtgcc gctttgctac
     6241 ttattaacgt atctttggta tgctccagcg tttcttttga aataaccccc tgcttcgcta
     6301 aaggcacacg acggttataa tcttctaaag attgttgata ctgaatacgt gctgaagcga
     6361 cttcggcact gtattgttta tcctgtaagt acagtttatt cgtttgccga acaatatttg
     6421 ccagattatt tttagcttta ttgagtgcga tagtggcatc agttttatcc agtgaaacta
     6481 aaatgtcacc ctgtcgaacg tagttcgtat ctttatgatt aactacagtg acactacctg
     6541 agacttgtgc agaaattgga tctgcattcc ccgtgacata ggcgtcatct gtactaatca
     6601 tgtcttctaa ttccattgac caataggcat aggcacctga aaacgcaata aataaaacta
     6661 tcaccaataa agaaaagtat ttttttctgt tagactgttt tttatttgaa ttaatctgtt
     6721 ccactattat ctctcatttc gcatagatga aatttatgaa ttgaatgaaa agagtattca
     6781 ttcttcatat caaacacagt gcatgtgttg cacattatcc caggaacgtt ggaggcctaa
     6841 tttaaatgag gtgcaaaaaa aacaacagag gactaaaccg tggcttttgc aatacaattc
     6901 ttacgcctgt aggattagtg agaagactta tagtgctaac ttgaaactat aaatcatcgg
     6961 tataatccct gattttattg ttgacatttc atttatgccg actatttata tggtatactt
     7021 gtcgaattat cttaaaggaa actcagattt tcttattttt attgagaaaa tgagatgacg
     7081 ccttatgtct gtattactac agggagaagg gagatgcttc attgcaaagg gaataatcta
     7141 tgaacgcaat aattattgat gaccatcctc ttgctatcgc agcaattcgt aatttattga
     7201 tcaaaaacga tattgaaatc ttagcggagt taactgaagg cggaagcgcc gttcagcggg
     7261 tggaaacact taagcctgat atcgtcatca ttgatgtcga tatccccgga gttaacggta
     7321 tccaggtgtt agaaacgctg aggaagcgcc aatatagcgg aattattatt atcgtctccg
     7381 ctaaaaatga ccatttttac gggaaacatt gtgctgatgc tggcgctaat ggtttcgtga
     7441 gtaaaaaaga aggcatgaac aatatcattg cggctattga agctgcaaaa aatggctact
     7501 gctatttccc cttctctctc aaccggtttg ttggaagttt aacgtccgac cagcaaaaac
     7561 tcgactcctt atcgaaacaa gaaattagtg tcatgcggta tattcttgat ggcaaggata
     7621 ataatgacat tgcggaaaaa atgttcatca gcaacaaaac tgtcagcacg tataaaagtc
     7681 gcctgatgga aaaattagaa tgtaaatcac tgatggatct ttacacattc gcgcaacgta
     7741 acaaaatcgg ctaatcacat gaagttttta ccctattttt ttcttctctg ttgtggtctt
     7801 tggtcgacca taagtttcgc agacgaagat tacatcgaat atcgtggcat cagtagtaac
     7861 aaccgtgtca cacttgatcc agtacgtctg agcaacaagg aattacgttg gttagcgagc
     7921 aaaaaaaatc ttgtgattgc agtacacaag tcccaaacgg ctaccttgtt gcataccgat
     7981 tcgcagcaac aggttcgtgg tattaatgct gattatttaa atcttttaaa aagagcgtta
     8041 aatatcaaat taacactccg ggaatacgca gatcatcaaa aagcaatgga cgcgcttgca
     8101 gaaggtgaag tcgatatagt gttatcacat ttagttactt cgccgcctct taataatgac
     8161 attgctgcaa ccaaaccatt gataattacc tttccggcgc tggtaaccac ccttcacgac
     8221 tcaatgcgac cgcttacctc accaaaacca gtaaatattg ctcgggtagc aaattacccc
     8281 ccagacgagg taattcatca atcatttcca aaagcaacaa ttatctcttt tacaaattta
     8341 tatcaggcat tagcatccgt ctcagctggg cacaatgatt actttattgg tagtaacatc
     8401 attaccagca gtatgatttc ccgctatttc actcactcct taaatgtagt gaaatattat
     8461 aactcgccgc gtcaatataa ttttttcttg accagaaaag aatctgtcat tcttaatgaa
     8521 gtactcaata gatttgttga tgctttaaca aatgaagttc gctatgaagt atcacaaaat
     8581 tggcttgata caggaaacct ggcctttctg aacaaaccat tagaactcac tgaacatgaa
     8641 aaacagtgga ttaagcagca tcccaattta aaggtgctgg aaaatcctta ctcgcccccc
     8701 tattctatga cggatgaaaa tggctcggtt cggggcgtta tgggggacat tcttaatatt
     8761 attaccttgc aaacaggttt aaatttttct ccgatcaccg tttcacacaa tatccatgct
     8821 ggaacacaac ttagccccgg aggatgggat ataatacctg gcgctattta tagtgaagat
     8881 cgagaaaata atgttttatt tgctgaagcc ttcataacaa cgccttacgt ttttgtcatg
     8941 caaaaagcgc ctgacagtga acaaacatta aaaaaaggaa tgaaagttgc cattccatat
     9001 tattatgagc tgcattcgca attaaaagag atgtatccgg aggttgaatg gatacaggtc
     9061 gataatgcca gcgctgcatt tcacaagatt aaggaaggtg aacttgatgc tctggtcgcg
     9121 acacagctaa attcgcgtta catgatcgat cattactatc ctaatgaact ttatcatttt
     9181 cttattcctg gcgttccgaa tgcatcgctt tcgttcgctt ttcctcgcgg agaaccggaa
     9241 cttaaggata ttattaataa agcactgaat gcaattcccc caagcgaagt tctgcgcctg
     9301 acggaaaaat ggattaaaat gcccaatgtg accattgaca catgggacct atatagcgag
     9361 caattttata ttgttacgac attatccgtt ttattagttg gcagtagcct tttatgggga
     9421 ttctacctgt tacgctcagt tcgtcgtcgt aaagtcattc agggtgattt agaaaaccaa
     9481 atatcattcc gaaaagcact ctcggattcc ttaccgaatc caacttatgt tgtaaactgg
     9541 caaggtaatg tcattagtca taatagtgct tttgaacatt atttcactgc ggattactac
     9601 aaaaatgcaa tgttaccatt agaaaacagt gattcaccct ttaaagatgt tttttctaat
     9661 gcgcatgaag tcacagcaga aacgaaagaa aatcgaacaa tatacacaca ggtatttgaa
     9721 attgataatg gcatcgagaa aagatgcatt aatcactggc atacattatg caatcttcct
     9781 gcaagtgaca atgcagtata tatttgtggt tggcaagata ttactgaaac gcgtgatcta
     9841 attaatgcac tcgaggtaga aaaaaataaa gcgataaagg ctaccgtagc aaaaagtcag
     9901 tttctggcaa cgatgagtca cgaaataaga acaccaataa gctctattat gggcttcctg
     9961 gaacttctgt cgggttctgg tcttagcaag gagcaacggg tggaggcgat ttcacttgcc
    10021 tacgccaccg gacaatcact cctcggctta attggtgaaa tccttgatgt cgacaaaatt
    10081 gaatcgggta actatcaact tcaaccacaa tgggtcgata tccctacttt agtccagaac
    10141 acttgtcact ctttcggtgc gattgctgca agcaaatcga tcgcattaag ttgcagcagt
    10201 acatttcctg atcattacct ggttaagatc gaccctcagg cgtttaagca ggtcttatca
    10261 aatttactga gtaatgctct caaatttacc accgaggggg cagtaaaaat tacgacctcc
    10321 ctgggtcaca ttgatgacaa ccacgctgtt atcaaaatga cgattatgga ttctggaagt
    10381 ggattatcgc aggaagaaca acaacaactg tttaagcgct acagccaaac aagtgcaggt
    10441 cgtcagcaaa caggttctgg tttaggctta atgatctgca aagaattaat taaaaatatg
    10501 cagggcgatt tatcattaga aagtcatcca ggcataggaa caacatttac gatcacaatc
    10561 ccggtagaaa ttagccagca agtggcgact gtcgaggcaa aagcagaaca acccatcaca
    10621 ctacctgaaa agttgagcat attaatcgcg gatgatcatc cgaccaacag gctattactc
    10681 aaacgccagc taaatctatt aggatatgat gttgatgaag ccactgatgg tgtgcaagcg
    10741 ctacacaaag tcagtatgca acattatgat ctgcttatta ctgacgttaa tatgccgaat
    10801 atggatggtt ttgagttgac tcgcaaactc cgtgagcaaa attcttcctt acccatctgg
    10861 gggcttacag ccaacgcaca ggctaacgaa cgtgaaaaag ggttaagttg cggcatgaac
    10921 ttatgtttgt tcaaaccgtt gaccctggat gtactgaaaa cacatttaag tcagttacac
    10981 caggttgcgc atattgcacc tcagtatcgc caccttgata tcgaagccct gaaaaataat
    11041 acggcgaatg atctacaact gatgcaggag attctcatga ctttccagca tgaaacgcat
    11101 aaagatctac ccgctgcgtt tcatgcacta gaagctggcg ataacagaac tttccatcag
    11161 tgtattcatc gcatccacgg tgcggctaac atcctgaatt tgcaaaagtt gattaatatt
    11221 agccatcagt tagaaataac acctgcttca gatgacagta agcctgaaat tcttcagctg
    11281 ctgaactctg ttaaagaaca cattgcagag ctggaccagg agattgctgt tttctgtcag
    11341 aaaaatgact aaattgcggc tcccacaatg ttctaatgtg ggagctattt accagcacat
    11401 cttacattta tgatgagaac tcctggcgaa tttgttcccc atgctggtcg agcgttgccg
    11461 ctcccggcat aacatgcggg tccgcgcagc cgctgatttt tatcggatta cccggcatca
    11521 ttattcctcc ggcttcaatc aacatatttc tcgcctgagt ttgtggcaaa ttaatggcct
    11581 cagccacact taataacggc gcgacgggca caccaacttc atgtattttg gctaaccaaa
    11641 cttcagctgc ctgcgttttt aacgttcgct caatatattg tttaagaata gcctggtttt
    11701 gtacgcgtaa aatattgctg ctaaatcggg gatcattaac cagttccgta agctccagtg
    11761 cctggcataa cgcagaaaaa agcttgtcat taccacaaca aatcgtaatc ggcttatcct
    11821 gagtatcgaa aacatcaaaa ggtgccatgt agggatggcg atttcccagg cgttgtggtg
    11881 acttccctgt cgcaatatat gccatcagac catgctccag aaaactcagc gtggcatcaa
    11941 acatcgctat atcgacatgc gcccctctct ggctcttttc gcggccataa agtgcactca
    12001 ctattccgct gaataaatag acaccgccgc atagatccgc aagagaggta ccaacacgca
    12061 ctggcggagc atcagggtac cctgtttcca tcattatccc actcattgcc tgaatgatgg
    12121 tatcgtaggc aggagcatct tttagcggac cggtatgtcc gaaacctgac gatgaagcat
    12181 atatgaggcg cgggttgatt tcttgtaacc tttcccatga aaaccccagt ttttccattg
    12241 tacctaggcg aaaattctca gctaatacat cagcttgttt gagcatattt ataaatatac
    12301 ttttatcgtg atcattcttt aaatcaagaa ccacactctc tttgccatga ttaataaaac
    12361 tgtaatagag tgactgtcca tccacatagg gaccaaatgt gcgggtatca tcaccatgac
    12421 ctggtggctc aactttaatt acccttgcgc ccatattata aagaagttga gttccgaaag
    12481 gtccattaag gacatgtgtc atatcgataa ctaataagcc ttcaaacggc cctttgcttt
    12541 cattatttgt catttgctta tgccttgaaa aaagccgaag gtgaaaagac acttcggcat
    12601 ttatgtaaaa tcaataacat gattaattat gaaaccagtc gactgacata aatccacaag
    12661 ggagccgtga cgacaaagcc cagtacgctc acagccaatg acgctgtacc agtacgggtg
    12721 tagacattaa accggctggc aataatgatt ccggagaatg ccggcggtaa tgcgcctgcc
    12781 agtaccatca tctgcaaatg ttcgctgttc aaatgacatg ccataccaac gagaagcagc
    12841 gccagtggca tcagaatcaa cttcaggaag gtgttataag caatttcggc actgaactcg
    12901 aatttatgtg cagccagagt caaccccgca gcgaataccg ctacccctga gttagccttc
    12961 gcaatcagat taaaggttgg gtcccatgct gccggaattt ttaccccaac cagcaccagg
    13021 atcgttgcca gaacaggtgc ccatactact ggctcctttg ccgcagaaat taatgcgctc
    13081 agattactat tcttcttacc atccgctcct gaggaaggat tcagcaaata cagaccaata
    13141 ggaatagtaa ttgcgttaac aataatagaa ataattgcta ccactaaacc tgttgatacg
    13201 gaatcaccat aaataggatc gagaactgca aaccccagga atccaatggt aggtgaaccc
    13261 gcaattaatg cacatacagc tgcttctgca tgggtacgtt taaaaaattt gtagcaacca
    13321 aaccaggaga aaaagaaaca tccgacaata accacaagtg atacaagggt cagacgagtg
    13381 tccgcaaaaa tcatttccct gtttgcccgg gtaatagaaa caaatagagc cgcaggaaga
    13441 gcgtagttta ataccagttt attaaatgcg cgggcttgat cttctgaaaa agtttctcgt
    13501 ctgccgctaa aataacccaa cagcatgatt acgataatag gcaataaatc gccaataaaa
    13561 aatgttagca taatagtatt tccgataagg gtcccctcac catcgcataa tgagttggca
    13621 agaggagacg atgtcagtat ttaattacca gcgacttgtt ttgggttaag tttggtaata
    13681 tggccacttt cagttcctgc tgcagggtcg ataaccacat taataatggt cggtttgcgc
    13741 gactgaatac cggtggttaa agcatgacga agttcatctg tcgtggtgac gttatagcca
    13801 acgccacgaa acgcatccat taatttgtca taccttgcat ggtgcaacag atccgttggt
    13861 gatggtgcac cagcgccact gagatcaaca ccgtctcctc tgtagatgcc gccattatta
    13921 aaaataacga tcgtcaccgg caggttatat cgacaaatcg tttcaatttc catcccactg
    13981 aaaccaaaag cactatcacc ttcaatggcg acaaccggag aaccagaagt cacgctagca
    14041 ccgatggcat agcccatacc gatgcccatg acaccccagg taccacaatc cagacgacga
    14101 cgtggtttat acatatcaat aatatttcgt gcattatcca gggtatttgc accttcatta
    14161 actaaataaa tatcctggtt ctcgcgcaat acgtcgcgca cagcacttaa tgcattaaag
    14221 taatttaatg gttgagtatc tgtacttaat ttttcatgca ttttttgtgc attttgctgc
    14281 ttgtggatat ttaaaatatc gcgccatacc agtggagtcg taaatgtgtt ttgtttcagt
    14341 tctgccagca taccttgcat actggatgca atatcaccaa cgactggcac agcaatgggg
    14401 cggttgctgt caatttcctg cggttcaata tccagttgaa taaactgtgt atctgccgcc
    14461 catccttttt taccgtgtgc caataaccaa ttcagtcgtg caccaacaag catgacaacg
    14521 tcagcatttg ccagggcaaa cgaacgcgca gctgccgcag aaagtggatg cgtatcttca
    14581 aggatccctt tcgccataga cattggcagg aatggaatct gagcactttc aataaattca
    14641 cgaagctgtt catcagcttg tgaatacgcc gcgcctttgc caaggataat taatggccgt
    14701 tcagcttttg ctaaaagcga aattgcgcta gtgactgact tcgggcatgg caataatgct
    14761 ggcgacggat tttcaacttt aacaatcgtg gttaacgctt cgtctttttc catcgtcgcg
    14821 gccaggacat ttgctggcaa atcaagataa actccgccag ggcgacccga tacagagacc
    14881 cggatagcgc gtgccaatgc aatgccaaga tcctgcggct gattaacgcg aaatgctgct
    14941 ttggcatacg gttttgccgc attcatttgg tccagctctt cataatcacc ttgctgtagg
    15001 tcgacgatcg cgcggtcgct ggagccgcta atcatgataa tcggaaaacc atttaccgtt
    15061 gcgttggcca atgcggtcaa accattgagg aatcctggcg cagaaactgt caggcagatc
    15121 cccggttttt gggtaagaaa accgcttgcc gcagcggcat agcctgccga ctgctcatga
    15181 cgaaaaccaa tataacgaat gccttccgcc tgggcatggc gtgccatatc cgtcacagga
    15241 atacctacaa caccataaat agtgtcaata ttattctgtt ttaatgcttc aacgatgata
    15301 tgcataccat ctgtcatttg aagttgatct gacatgatta ataacctcga aatttttata
    15361 aattgggagc cagaagcccc cgttaatatc agatggcgtg gttttgcttc attgcagcaa
    15421 tttcatcgtc gctataaccc agctcctgca atacagcagc ggtatgttca cctaatagcg
    15481 gcgcagcttt aatatccggc gtaaaggcag agaatttcat tggacaacca acggtcagat
    15541 attttccacg caacggttgt tccacttcaa caacactgcc actttggcgc aaagagggat
    15601 caagtgaaat ttctttcata cttaaaaccg gtgcacaagg aatatcaaac tgagtcaaat
    15661 aggccaccgc ttcatgttta tcaatagtga cagtgtattt ttcgatttca gcaaaaatat
    15721 cgaaaatatg tggctgtcgg gcatgggctg tactgtatgc cggatcggta atccattctg
    15781 gtttgccgat ggctttacag gtgttttccc agttttgctc ctgaatagtg aaataaatat
    15841 aggcgttagg atcggtttcc cagcctttac atttcaggat ccagccaggc tgaccgccac
    15901 cacctgcatt accaccgcgg ggaactgcat caccaaatgt accattcgga tactgcgggt
    15961 attcttccag ataacccaat ttatcgagac gctgctggtc acgtaatttc acgcggcaaa
    16021 ggttcaatac ggcatcctgc attgacatgg tgactcgttg cccacgcccc gttttttcgc
    16081 gatgcagcaa agcagcaagt aaaccgatca gcaaatgcat tccggtgttg ctgtcaccca
    16141 acgctgcagc gcttaccagc ggcggaccat cccaaaaacc cgtagtggat gccgcgccac
    16201 ccgctgcctg agcaacgttt tcataggctt ttacattcac ataaggcgaa cactcatcaa
    16261 accctttgat cgaaccaaaa atcagacgtg gattgatttc ttgaatatgc tcccaggtga
    16321 agcccatgtg atcaatggcc cctggatgaa agttctcgac taagatatca gcttcgcgga
    16381 tcagcttttc cattacctct ttgccttccg ctgttttggt atttaactca atagaacgtt
    16441 tgttactgtt aagcatggtg aagtaaagcg catcgatatc aggaatatct cgcagttggt
    16501 gacgcgttac gtcaccaacg ccgggacgtt caattttaat aacgtcagcg ccaaaccagg
    16561 ccagcatttg agtacaagat gggccagatt gcacaccggt gaaatcgaga actttaattc
    16621 cttgaagtgg agttgacata gcaataccta ttagttaatt aaatatgtgt taatttagcg
    16681 tttatttaag gcataacgcc ctctataaat aatctgcaaa atatcttcgt agcggcactc
    16741 gatattttta aattcctgta tcaagtactg aaaattattt tatcgccact tccaattcct
    16801 ttctgcatgg taagtagtga ggacgaaaag aagaatatga taaatattaa ttcgtggtaa
    16861 tcagagagag tcgcgacatt tgtgatgtta ttcttacaac tgtaagaagc gccggtgatt
    16921 tacaagtaag agtatgatta cgctttttaa agcgtaccat aaagcataac ataataaatg
    16981 atttgtgatt acacgggatt attgcggttt actttgagga aaataaaatg cgtgtttata
    17041 gaaccgcgag cgccaaataa ttttatgacc acaacctgtc gtattgtggt cataaaaaat
    17101 tgtcggctgg agggagttaa atcctttacc tcaatatact gcctgtcagc attagttcac
    17161 aaacagtgct tcgctatcga cgatgattcc atcttccgca cctttaagcg ccaggtttgc
    17221 ctcgtagtat tgctttttat caagcagttt ctgcgcatca gcaagtgcat tacgagtctg
    17281 tttaagcggc atcagatatt ggttttccat tacgccaaca ccggccaggc gaagctcttc
    17341 cattgcgccc tttttatccc ctttcgccat tttttcattg gcaattttta tcgcggcttc
    17401 tttttcgggt gttgctacat aactctcaga aattccgaca gatgcattaa tgacaatata
    17461 ttggtcatca ttgagattgg tctttttacc tggcttagcg aactttgccc attcggtact
    17521 gtcatctgac agcaaagcgg aagcttcatt ggtcagttct tttgcctttt ccggatcgcc
    17581 atgaaacaga gccaggcgcg ctacctgcac gtcacgcatc gcatataaac cttgttcaga
    17641 gatacgctct gctgctgccg ttttctgggt ttgctgagtt tgctgttgtg ccgctaccgg
    17701 tgcattatca gcagcccata cagttgaaga tgccagaatt gctgtgacca tcgtagccat
    17761 aattaaacgt ttcatactta cctcattctt taacagtttt agaaaacgcc gccatcatat
    17821 tgggtgagca tgaaatgaga aatcagagag caacggtcat tttgaaaggg ttatcttaca
    17881 attgtagtta ttatcgcaaa gcggcattta gtcttacagt tgtaggaata tgcctcctgt
    17941 cgcagagaac tattcttata taccacaaag agatgtcatt tattatattg atgtaaatat
    18001 tgattaatta tatgtaaata cagccataac atcgtatggt tctcagtgaa ccattttcag
    18061 gataactttc tatgaaagta aacttaatac ttttcagctt atttttattg gtctctatta
    18121 tggcatgcaa tgtttttgca ttttccattt ctggtggtgt aagtgagagg agctataaag
    18181 agactgaaaa aacatcagcg atgacgacca cacactctac aaaacttcag ccatcacagg
    18241 cgattttgtt taagatgaga gaagatgtgc caccattaaa cctcacagaa gaaatcacgc
    18301 ctacttatcc gacaaaggcg aattatctta ttcatcctgt gcgatagcgt ctgtcacatg
    18361 gcaggtgaac aatcatgtct gatgctgatg tgaacacctg cccaaatgag ccatcctgac
    18421 ccactatcgc ctgccatacc ccaccataaa gcgaatcatc aagccacaag ccggggcaag
    18481 aaacataaat atccccgtca ccagttcatt accgccagtt aaccacatgg tgaagtaata
    18541 aatgagtgca gcgaggatca ccatgccgaa gaaagaagag accgccgata ccacgttcag
    18601 tacctgaccg atgtagccgc ttacgcagac aatacacagg gcgagaaaca tccataaata
    18661 aatcatagct caacctcctg ccgcgctaaa aaacctgtaa cttcagtcac tttaatagta
    18721 agacaatcct taacctctgg caatttttgc taagcatcaa tgaaaacagc acgttaaatg
    18781 caacgctatt gcgtgcggaa aattttttac tttgcacgat taataatcag tagctgaaag
    18841 cagtcagcca gtgataaagt agagccaccg cattcatgct cctcgtggtt atgtcctgac
    18901 tagtctttac actctttaca ggaaccattg tcgtacatga tggcccaacc aattgagtgt
    18961 accgctgtcg acatggaatc ttctgcgata caacaattcg tatctacaga aggtaactat
    19021 gtttccacaa tgcaaatttt cccgcgagtt tctacatcct cgctactggc tcacatggtt
    19081 tgggcttggt gtactctggc tttgggtaca gcttccttat cctgttctct gctttctcgg
    19141 cacgcgtatt ggcgcaatgg cgcgaccatt cctgaaacgt cgtgaatcta tcgcccgtaa
    19201 aaacctggaa ctttgtttcc cgcagcattc tgcggaagaa cgcgagaaga tgattgccga
    19261 aaactttcgt tcactcggca tggcgctggt agaaaccggc atggcatggt tctggcccga
    19321 cagtcgcgta cgtaaatggt ttgatgttga agggttggat aaccttaaac gcgcacaaat
    19381 gcaaaatcgc ggcgtaatgg ttgtcggcgt ccattttatg tcgctggaac tgggcggccg
    19441 cgtgatggga ctgtgccaac caatgatggc tacctatcgt ccacataata atcagctgat
    19501 ggaatgggtg cagacccgtg ggcgcatgcg ctctaacaaa gcgatgatcg gcagaaataa
    19561 tctgcgcggc attgtcggtg cactgaagaa aggtgaagcg gtatggtttg ctcccgatca
    19621 ggattatggt cgtaaaggca gctccttcgc gccgttcttt gcggtggaaa atgtcgccac
    19681 aaccaatggc acctatgttc tctcccgtct ctctggcgca gccatgttga ccgtaacgat
    19741 ggtaagaaaa gcggattaca gcggatatcg tttgttcatc accccagaga tggaaggcta
    19801 cccgacagat gaaaatcaag ccgctgctta tatgaacaag attatcgaga aagagatcat
    19861 gcgcgcaccg gagcagtacc tctggatcca ccgtcgcttt aaaacgcgcc cggtgggaga
    19921 atcgtcgttg tacatttaac aaactgctct tactctttcc ctcctcaaat cggccataat
    19981 aacctcatga tttttatagc ttttttctta agttttttcc ttcgctgaat atctaattat
    20041 taccgtcaga aattaaactg tcgccgatat gagacacttg taagttacta aaagtaaata
    20101 agaaaatgcc tcttgtctcg tttcagtttt aggcgtacat tagcgccgtc tcgagcagtt
    20161 tagctcagaa ttctggggtg atgcccacgg taaaatacag ggcttttaag gtcgctttgc
    20221 ccggtgtcaa ctcactgtat caggtgtaat gaagtcattc aggcgtaaca gtaattacgc
    20281 ggagagatgt aaagtgaaat atttctttat gggcatttct tttatggtca tcgtttgggc
    20341 cggtactttt gccctgatga tctaaaagca aactgtgcaa tgaaaaacag gagccatcag
    20401 gctcctgttt gcatttctga cgacggtgtt tattccgcgt tttcgtgaat atgtttgctg
    20461 ctgacgggta gtaaaccgtc ggcacggaac atcgctttaa tccctctgat ggcctgacga
    20521 atacggtcgt ggttttcaat cagtgcaaag cgaacatgcg tatcaccata gtcgccaaag
    20581 ccaatccccg gcgagacaca aaccttcgct tcgttaagga gcttcttggc aaattccagc
    20641 gatcccatgg ccgcatatgg ttccgggatt ttcgcccaga catacatcga agccttcggc
    20701 atttcgacca tccagcccgc ttcatgcagc cctttaacca atacatcacg acggcgtttg
    20761 tactgttcgg caatgtcgcg cacgcactgt tgatcgccct cgagtgccgc aatcgccgcc
    20821 acctgcaacg gcgtaaaagt gccgtaatcg tgatagcttt tgattcgcgc cagggcactg
    20881 accagcgttt tgttgccgac cataaagccg atacgccagc ccgccatgtt gtagcttttc
    20941 gacagcgtaa agaactcgac cgccacatcg cgtgcacccg gcacctgcat aattgacggc
    21001 gctttccagc catcgtagac gatatcggca taggccaggt catggaccac cagcacatcg
    21061 tagcatttcg ccagcgccac aaccttttcg aagaactcca gttccacgca ttgcgcggtt
    21121 gggttcgacg ggaagccgag gatcatcatc ttcggtttcg gataactttc acgaatggcg
    21181 cgctccagtt cgttgaagaa atcgacacct tccaccagcg gcactgagcg tacctgcgcc
    21241 ccggcaatca ccgcgccata aatatggatc gggtaacttg gattcggcac cagcaccgtg
    21301 tcaccatgat ccagcgtcgc cagcatcaga tgcgccaggc cctctttcga accaatagtg
    21361 acgatggctt ctgattccgg gtcgatttca acgtcgtagc gatcctgata ccagcgggaa
    21421 atggcgcgac gtaaccgcgg aatgccgcgt gaagtggagt aaccatgcgt gtccgggcgc
    21481 tgggccacag tacataattt ttcgacgata tgcggcggag tcgcaccgtc cgggttaccc
    21541 atgctgaaat cgataatatc ttcgccgcgc cgacgcgcag ccattttcag ttcagcggtg
    21601 atgttaaaaa cgtagggcgg gagacgatca atgcgcgtaa agcgacgttc agggcgagtg
    21661 tcagccataa tttcctcaga ttaacgttag cgcccggacc gtccgagcga cgctgccaca
    21721 atggtggctg ttttgaaaat agcctgatta atttctgtct gtccagcgtt ttagaacatt
    21781 tttatcaatg gatttaaatg aggaactcca cttcaaaaac aggaaatttt tctacgctaa
    21841 caagaaaatc accaccaatt ttattaacat tataatttca ataaatttac cagccagaag
    21901 tgcattatca agagatacat tgctaataca ccatttatct tttaatcccc ttttaaattt
    21961 atggtttttc ctcaaaaacg gcctgctggt catcagcctt caggttacct atcatagagg
    22021 tttaatcctt attcagagtc acccgtgcac gaaatattca acatgctgct ggcggtcttc
    22081 gatcgggcag cgttaatgct tatctgcctg ttctttctca tccgtatccg cctgtttcgc
    22141 gaactgttgc acaagtcggc gcactcccca aaagaattgc tcgccgttac cgccattttc
    22201 tcgctgttcg ccctgttcag cacctggtcc ggcgttcccg tagaaggctc gctggtgaac
    22261 gtacgtatta tcgcggtgat gtccggcggg attctgtttg gcccgtgggt aggcatcatt
    22321 accggcgtga ttgcgggtat tcaccggtat ttaattgata tcggcggcgt gacggcgatc
    22381 ccctgcttta tcaccagcat tctggcgggt tgtatatcag gctggataaa cctgaaaatc
    22441 cccaaagcac agcgctggcg ctggatatcg ccaacaaaac gctaccgctg tttcgccatg
    22501 tcaatagcga gtcattacgc aaggtctgcg aaattatccg cgatgacatt cacgccgatg
    22561 cggtggcgat tactaatacc gatcatgtgc tggcctatgt tggcgtgggt gaacataact
    22621 atcagaatgg cgatgacttc attagcccga ctacccgtca ggcgatgaat tacggaaaaa
    22681 tcatcattaa aaacaatgat gaagcccacc gcacaccaga gattcattcc atgctggtga
    22741 tcccattgtg ggagaaaggg gtcgtgaccg gaacgctgaa aatttactac tgccacgcgc
    22801 atcagatcac ctcgtcatta caggaaatgg cggtcggtct gtcgcaaatc atctccacgc
    22861 aactggaggt ttcacgcgcc gagcagctac gtgaaatggc aaataaggca gagattcgcg
    22921 ccctgcaaag caaaattaat ccccattttc tgtttaacgc tctgaacgct atttcatcgt
    22981 caatccgtct gaatccggat accgctcgcc agttgatctt taatctgtcg cgttatctgc
    23041 gctataacat tgaattaaaa gacgatgagc aaatcgatat caaaaaagag ctgtatcaaa
    23101 ttaaagacta tattgccatt gagcaggccc gctttggcga caagctgacg gttatctatg
    23161 atattgatga agaggtgaat tgctgcattc ccagcctgct gatccagccg ttggtggaga
    23221 acgccattgt ccacggtatt cagccttgca aaggtaaagg cgttgtcacc atcagcgttg
    23281 cagagtgcgg aaatcgggta cgcattgcgg tgcgagatac cgggcacggc atcgttccaa
    23341 aggtgattga gcgggtcgaa gcgaatgaaa tgccgggcaa taaaattggc ctgctgaatg
    23401 tccatcatcg cgtgaagtta ttgtatggcg aggggctgca tatccgccgc ctggagccgg
    23461 ggacggaaat tgcgttttac attcctaacc aacgcacccc agtcgcctca caggctacgt
    23521 tattgctttg agccggagtg atattgtgaa agtcatcatt gttgaagacg aattcctggc
    23581 acaacaggaa ctgagctggc taattaaaga gcacagccag atggagattg tcggcacctt
    23641 tgacgacggt ctggacgtgt tgaagttttt gcagcataac cgcgtcgacg ccatttttct
    23701 ggatatcaat attccgtcgc tggatggcgt gttgctggcg caaaacatca gccagttcgc
    23761 ccataaaccg tttattgtgt tcatcaccgc gtggaaagaa catgcggtag aagcgtttga
    23821 actggaggcg tttgactaca ttctcaaacc gtatcaggag tcacgtatta ccgggatgct
    23881 gcaaaaactg gaagcggcct ggcaacaaca gcagaccagc agtacgcctg ccgcgacggt
    23941 aacgcgtgag aatgacacca ttaatctggt gaaagatgag cgaataatcg tcacgccaat
    24001 taacgatatc tattacgccg aagcgcacga gaaaatgacc tttgtctata cgcggcgtga
    24061 atcctacgta atgccgatga acattaccga attttgcagc aaactgccgc cgtcgcattt
    24121 tttccgctgc catcgctcat tttgtgtcaa tctgaacaaa atacgcgaaa tcgaaccgtg
    24181 gtttaataac acctacattc tgcgactgaa agatctggat tttgaagtgc cggtcagccg
    24241 cagcaaagtg aaagaatttc gccagttaat gcatctttaa tcgggaactt tcatgaaagc
    24301 accaggcttg cccgccgatc aacaattttt cgccgatctg ttcagcggcc tagtgcttaa
    24361 cccgcaacta ctggggcgcg tctggtttgc cagccagcct gcctcgttac cggtgggcag
    24421 tttatgtatt gattttcccc gtctggatat cgtgctgcgc ggcgaatacg gcaacctgct
    24481 ggaagcaaag cagcagcgaa tggtggaagg agaaatgctg tttattccgg cacgcgcggc
    24541 taatttaccg atcaacaaca aaccggtgat gctgttaagc ctggtgttcg caccgacctg
    24601 gcttgggtta tcgttttacg atagccgcac cacgtcgttg ttgcatcctg ctcgccagac
    24661 ccagcttccc agcctgcaac gcggtgaagg tgaagcgatg cttaccgccc tcacccatct
    24721 tagccgttcg ccgctggagc agaatatcat tcagccactg gtgttaagtt tgctgcatct
    24781 ttgccgcaac gtggtgaata tgccgccggg taattcacaa ccgcgcggcg attttctcta
    24841 tcacagcatt tgtaactggg tttaggataa ttatgcccag ccgctcaccc gcgagagcgt
    24901 ggcgcagttt tttaatatca cgcccaatca tctgtcaaaa ctgtttgctc agcatggaac
    24961 gatgcgtttt atcgagtatg tgcgttgggt gcgaatggcg aaggcgagga tgattttgca
    25021 gaaatatcat ctgtcaattc atgaagtggc acagcgttgc ggttttccgg atagcgacta
    25081 tttttgtcgc gttttccggc gtcagtttag tctgacgccg ggagagtaca gcgcccgttt
    25141 tcagggctaa cgtcagaagg ttaattctgt ttccagcagc gtcaggatac tttgtgcatc
    25201 ttgcgcggca aacaacgact ggcggaagtt tttattcacc agtttacgcg ccagctgcga
    25261 gaagactttc acatgattca tcccttcgtt agcacccagc gtcagcatga tcaccagttc
    25321 gacttcgccc atttctgact gccagtcaac cggtttcgcc agccgggcaa tgctgatact
    25381 ggaatgacgg atccactgag atttggtgtg cgggatcgct acgccaaaac caacgccggt
    25441 ggtaacaatc tcttcccgct gccagacatc ttcttccagc tcaaacggat gttcagtgcg
    25501 cccgttaacg ccgaggttgc cgcacaggaa ctggatcgcc tgctctttat tgctaaaatc
    25561 ctgatcaaca aagatattct ccagcgccag cagtgggcga acgtcttctt ccggcgtaaa
    25621 ggcggtgagt aacgcttcaa tttcctgcgc actgcggcat tcacatgcct gacgcgccag
    25681 ttcccgacac gcctcgctat ccagttgacg aagctggctt ttcaccgccg gaatacgcgg
    25741 gctactcata ctcagctcat ccaggcccag cccaagcagt agcggcagat aacggctttc
    25801 accgcccagt tcaccgcaaa tgcctaccca tttgccccgc tgatgcgcag tggtaactat
    25861 ttgctgcaac atgcgcagga acgatggcgt aatcgggtta tatagcggcg atacgcgcgg
    25921 gttattacga tcgaccgcat acagatactg ggtcatatcg ttggagccga tactgaagaa
    25981 atcgacctca tcgcagaagt ggtcgatgat gtagcacacc gacggcactt ccaccatgat
    26041 cccaagcgta atcgtctctg catgacgcag gccatcgcgc ttaagctcaa cgatcgcttt
    26101 ttggatctcg cctttcaccc ataagatctg atcgaggctg tgaaccatcg ggatcatcaa
    26161 ctgggcgttg ccgaaactgg cggcgcgcaa aatggcccgc agttgagtgc ggaacaggcc
    26221 agcaaattcc gggtaaatac gtaccgcgcg atagccgagg aacgggttct cttcctgggg
    26281 aatattcaga taaggaatgc ttttatcgcc gccgatgtcc atcgtgcgga agataatcgg
    26341 cttgtcgccc gccgccagta gcacctgctg gtaggcttca aattgctcct gctcgtccgg
    26401 cgcgctgtcg cgatccatat agagcatttc ggtacggaac agcccgacac cttccgcgcc
    26461 gttggcaaac gcgcctggcg cttccagagc ggtgccgata ttcgccgcaa tatcaatacg
    26521 cttgttatca cgggaatagg ccagctgcgc ggcagcctgc gcctgttgtt tttggcgttt
    26581 atccgccagc gtctgcgcga cctgataata accgctcacc gcgtcattcg ggttaatcgc
    26641 cagcacgccg cactgggcgt caagcactgc aggttgcccg gcataacggg caatcgcatc
    26701 cagcggcaag ccactcagta ccgggatcgc cgaggcacgg gccagaatca gtgtatgcga
    26761 ggtgcgcccg gttttctcca gaatcatacc cgcaagattt ttcaaatcaa ggctcaaaaa
    26821 ctggcttggg gttaaatctt cagccaccag aatggtcggt ttttccagca ccagattgtt
    26881 gcgcggcttc agttccggcc aggtgatatg cagcaactgt tcgctgatat cgcgaatgtc
    26941 gctgacccgc tcgcgcagat aatcgctggc agaagcagat agtttggcgc aaacctgctc
    27001 catattgctg atgatcgccg cccccagtcc ctgatgctgt tctgtcatca ggcgacggat
    27061 attgcctgca aattcatcat cctgaatcag cgacaaatga gcgctgagga tagttttgct
    27121 ttcgccgtca cgctcacgca attgctgatt cagttgctcg gcaagcgttg ccaggctgtg
    27181 ctccagccgg gtggaatctt gcgcactggc ggggattgcc cgataactgt cgaggctgtc
    27241 gctttgtaac agggtcagcg tacccacgcc gacgccgctt gccagcacat tgccgtacag
    27301 taaatccggg ttaaggcggc ttaatgaacg cggcagcgga tgcgccgtca gttctgcctg
    27361 cgtcggctga acgctgtcgc tgtcgataaa gcgcaccagg atgtactctt ccagcacgcg
    27421 ccgcgcctgc tcttcatcgc tgccgctaat gttcaggctg caactgtcat taaataaggt
    27481 gccagttcca atcaacgcca gcgagctttt agcatcagcc tttgcgttct ggcgatggtt
    27541 gataaaggtg atttcgcttt gccactggct gcactgttct ttaagttccc acgccggacg
    27601 agcatgtaga ccgttaggca aaggacagag aaattgaatc gttaacataa ccgctccggg
    27661 ttaatcagga gatcatctga aatccgtcag ttgaacaacc gtctcacgcg taagacgttg
    27721 aataagggca gataaaagtt gctccatctg caaaatgtcg cggcaatcgg caatcgatgc
    27781 cgcgcaatgt ccgtggcggg tggcaggccc cattaccaat gtgggtacgc cagtaccggt
    27841 taagtgcacc gctccaccgt ccgtgccgcc gttactgaac atatccgcct gcaacggaac
    27901 gccaatttct gccgccacgg tttcaatcca cgcggtaagt tttggcggcg caatcagcga
    27961 tttatcgctt aacaccagca tcggcccgtt accaatctga cgatggttag ccgcgccata
    28021 atcaaagttt ttcgcccagc aggcggtatc aagcacaatg gcgacgtccg gcgacaccgc
    28081 gcgggtagca gtttgcccgc cgcgtaatcc cacctcttcg ctggaacttg ccaccagcca
    28141 cacttccgca ggcagttcgg cgtcgtgcag ctcacgtagt aacgtcacca gcagatagca
    28201 actgaggcga tcatcaaagg ctttgcccat cacccgctgg tgagggagga cctgaaaagt
    28261 ggtatcgaag gtgacgcgat cgcctggacg aattcccgcc tgcatcactt cgtcataggt
    28321 gcgcgcgcca atgtccacgc gcatggcgct aacgtcattc ccctgccggt cgccgtcaag
    28381 caggcctgga attttgcact cttcacgggt ggtgatgcgc accggctgca gctggcgggc
    28441 agccatgcgc acgttgccaa ccggcagcac gtcaatagcg ccttcgcggg agatgctgcg
    28501 caccataaat cccacttcgt ccatatgggc gcagatcatc accttcggac ctgtcgattc
    28561 attgaggcgg atcagcaccg atcccagacc atcaaatcgc acttcttttt gcaggcgagc
    28621 cgcttcttcc agcaggatct gccgcacttc ctgttccgag gaggcgatcg catctgcctc
    28681 gctcaacgct tttaatagcg ataaatccat tatgcctctc ccgtgagcaa cactgttttc
    28741 ggcatggcgt agagcacttc tgcgccttgc ggggtaacca gcacaacatc ttcgatgcgc
    28801 acgccccctt gccctagcaa ataaatcccc ggctccacgg tcagcaacat gcctggctgt
    28861 agcgtcgtgg tgtcccgcgg tgaaaaacgc ggatcttcat ggacttcaat gccgatagcg
    28921 tgaccagtgt tatgaccgaa atagtcgcca aatccagcct cggtaatgac ccggcgcgcg
    28981 gcgtcgtcaa tctgctggca gcgcacgccg gggcgaattg cagagattgc cgcgagctgt
    29041 gcctgcagga caatctgata gacgtcaaac agcgggtgag attcggcgct caccccttcg
    29101 ccattcacca gcaaggtgcg cgtcatatca gcagtagccc tgatacagcg caccaaaatc
    29161 gagagtgaca aactcgcccg ctgcaacaat cttgtcgctg gctttgccgt gcggcagcgc
    29221 cccgcgccag ccgctggcga caatggtgtc aaaagaggct ttttctgcgc cctgctggcg
    29281 cataaaccac tccagttcag cggctatctc gcgctcgctc atccccgcct gaataaagcg
    29341 gcgaatatgc tccgctccgc gatcggcaat cccacaggcg aggcggatta tctccacctc
    29401 ctctggcgtt ttgatttgcc gcagcacatc cggcgtggcg ctaaccagtt tcgcattgag
    29461 ttcagacttc cagcggtgcg cggtttccca actcacctgc tggccttcaa aaccgagcgt
    29521 ttgcaactgc tcatcggcaa tgatttgatt gacgatagtg gtaagcgtgt tcgttgcgtc
    29581 aagcaaatgc agctggtagc cttgcgcacg ggcttccacc tcaacgaaat agcgcgaatc
    29641 taccagaatg tgcgcacttt cacggctaat aaccacatag cctgatccgg tggagatccc
    29701 cagatgcggc tgtttgttct gccgtgagga gagaagcacc gcatccagtt gttgcacctt
    29761 aagccagttg cgcagcgaag cgagtaatgt catctggatt ttccttctta caggctatcg
    29821 attaacaatt tgcctttgcg gaacatcatc agacgcagga acaccaccat cagcgcagta
    29881 atgattgccc ccagcgcgat ccccgccata taaacgccca agttagtgac cagcggccat
    29941 gcccagatag cggattccgg gaaccattgc actgcgccca gccagacagc tgcggttgag
    30001 ccgacaatcg cgccgaccat atatgacgga atagcggtga tggggctttc cagcgcaaac
    30061 ggaatcgcac cctcactaat ccccataaag gccaggaaca tcgcggtctt accctgcgga
    30121 taaagttgag cgttgaacag gcgtttgccg gttaaacgac ggtcaataat ggtcgccaga
    30181 cccagaccaa tcggcggaat aacgatagcg atagaacgag cggtgaccgg taatacgtgg
    30241 tcagtggtaa agctgaaggc aacgaaacct gcggctttgt tgatcgggcc accaaggtcg
    30301 attgctgtcg ccgcggcgat ccccattgcg tacatcagcg cgcccttctc acctgcggca
    30361 gtcagcacag tacggatacc gccgttgatc cagccaccaa agggagtgat gacgtagtac
    30421 atcgccagca tgacaaaaat ggcggaaaga atcggtagca aaaatgtggt tttgaacgct
    30481 aacaggaaat ccggtaactg gattttctgg ttcatccatt tcacaaggta accggcaacg
    30541 atagaaataa tcagcgcacc aatgaaggtg gacggcaccg gcgaagaggt cgcccattgc
    30601 atagtgctgg gatcgaagtt cagcagttgc gtcggctggg tagacatcaa cccgccgata
    30661 aaaccagccg ggaacgccag tttgccaccg atagagttcg ccacaaatgc agcaaacatc
    30721 ggaatggcaa aaccaaacaa cacgccgccg aaggactgcg acagccaggc aaatttaagt
    30781 aaggagaggt cgaaaccgga gaatttcccg ctattaagag catccatgat gccgatctca
    30841 gcaggaattt taagccagct ataagcaatc agctggctga aagcgaggat cacaccgccc
    30901 ataattaagg tcggcaccat gcgtgaaatc cctgacatca catgctgcgg gagttcaccc
    30961 cagaagctgc ttttagaggc ctgcggattc ttaactgccg ctgccgcacc ggatgcgcca
    31021 ggcacaacgg ttgcactgcg ttttttaatg gccataacaa ttatccctgt cgaatattac
    31081 tgttgttcag aggcaatcat ctcttcgatt tctttgatga tgcccgcagc gtttttaatt
    31141 gcgtcctgca aagtgatttc ataaacgtcg cgtgattcga aacgttcgtt atcttccggg
    31201 gtaactgcca cggaatggat gatgatggtc gcttcggcga tatcctgcgc cgtcaggcga
    31261 ttctggatgc cgtccgcgcc ctgggtttca attttcactt cataaccggc ttctaccgcc
    31321 gcttcttcca gcacctgagc ggccataaaa gtgtgagcca ggcccattgg gcaggcacat
    31381 aaggcaatca gtttcttact catcgttaat tcctcgttca gtgatagttg actagtgaaa
    31441 ttatgaacgt gcaaaacaaa tcgccgttac cagacaaata atgcatttac tggagaatta
    31501 atcccgctgt acggaagtgt gacgcaggtc ggccttgtga ggggacacaa agaaaaattg
    31561 aattaactgt atgatttaaa agattatcgg gagagttacc tcccgatata acaggaagga
    31621 tttacagaat gtgacctaag gtctggcgta aatgtgcacc ggaaccgaga agacccggat
    31681 agtcatggac gatgagatac accggaatat catggacata ttctttaaag cgccctttat
    31741 cttcaaatgc ggcacggaaa ccggaggctt tgaagaactc aaggaagcgc ggcacgatac
    31801 cgcccgcaat aaacacgccg ccaaatgtcc cgagattgag cgccagattg ccgccaaaac
    31861 ggcccataat gacgcaaaac agcgacaatg cgcggcggca atcggtgcag ctgtcagcca
    31921 gcgcgcgttc ggtaatatct tttggcttga gattttctgg caggcggttg tcagctttca
    31981 caattgcgcg atacaaattc accagcccag ggccagaaag cacgcgctcc gccgaaacat
    32041 gaccaatttc cgcacgcaat atttcgagga taatggcctc ttcttcacta ttcggcgcaa
    32101 aatcaacgtg accgccttcg cccggcaagc ttacccaacg cttatcgaca tggaccagat
    32161 gcgcaacccc aagccccgtt ccggcaccgt aaaccgcaat aggcttacct tcgaccggtt
    32221 ctgcgccacc aaactgaatc agatgctctt ttttcagcat cgggatcgcc atcgatacag
    32281 cggtaaaatc gttaataatt tccagatggc taaaaccgag attctttttc atttcggcaa
    32341 ttgagaacgc ccaggtatgg ttggtcattg ccacccagtc accggtaatt gggcaagcga
    32401 tggcaataca gccgtctttc acctcgacct tatgttcttc aagataaacg cgaatgaccg
    32461 cttcgaggct ggggtaatca agccctgaat aggtcttagc ctgcgagatt tcaccactgg
    32521 caatatcaca cagagcaaga cgtgcgttgg tgccgcccac atcaccgact aatgcatact
    32581 ttgtcattct tcaactgctc cgctaaagtc aaaataattc tttctcacac tgtaaatacc
    32641 tgggggcata acaacaacgc cggaaaggca ggctccctgt aaatatcgat ctgggtcaca
    32701 caattacttt atcgtttcag caccaattgc agcgatgcct ttttgcaagc tgggcaaact
    32761 aagtatctga ccccgcataa ggaatagaac atgctccatc cgcgagccag aaccatgttg
    32821 ttattatcgc tccccgccgt ggcaattggg attgcgtcca gtcttattct gattgtggtg
    32881 atgaaaatcg cctcggtatt acagaatttg ctctggcaac gactgccggg aactctgggg
    32941 atagcccagg attcacccct ctggatcatc ggtgtattaa cgctaacggg tattgcggtg
    33001 gggttggtta tccgtttcag ccagggtcat gccggaccag accccgcctg tgaaccgctg
    33061 atcggcgcac cggttccgcc ctctgcgcta cctagactta tcgtagcatt aattctcggt
    33121 cttgctggcg gcgtcagcct ggggccggaa catccgatca tgaccgtcaa tatcgccctt
    33181 gccgtcgcca ttggcgctcg tctgttaccg cgcgtcaacc gaatggagtg gactatttta
    33241 gcctctgccg gaaccatcgg cgcgctgttt ggtacacctg ttgcggcggc gttgatattt
    33301 tcgcaaacct taaatggcag tagtgaagtt ccgctatggg atcgtctttt tgcgccgtta
    33361 atggcggcag cagctggtgc acttactacc ggattatttt tccatcccca tttttcactg
    33421 cccattgcgc attacggaca gatggaaatg accgatattc tcagcggtgc aattgtcgcg
    33481 gcgattgcca tcgcagcagg gatggtcgcc gtatggtgct taccacggtt gcacgcgatg
    33541 atgaatcaaa tgaaaaatcc ggtgctcgtg ctgggtattg gcggatttat tctcggaatt
    33601 ctgggggtta ttggtggacc agtttcgctg tttaaagggc tggatgagat gcagcagatg
    33661 gtggcaaatc aggctttcag caccagcgat tactttttgc tggcggtaat taaacttgcc
    33721 gccctggtcg ttgctgccgc cagtggcttt cgcggtgggc gaatcttccc ggcagtgttt
    33781 gtcggcgtgg cattagggtt gatgctgcat gagcacgttc ccgccgttcc agcggcaata
    33841 accgtttcct gcgctattct cggcatcgtg ctggtggtaa cacgcgatgg ctggttaagt
    33901 ctttttatgg cggcagtcgt tgtacccaat accacattgc taccgctgct ctgtatcgtc
    33961 atgcttccgg catggctgtt attagcaggt aagccgatga tgatggtcaa tcgtccgaag
    34021 caacagccac cccacgataa tgtttagcaa aaaatgctct cctttatgat ttaagagtta
    34081 tggcgatgat acggggcttt acctccccgt aatattgcgt taacaggccg ctgacggctt
    34141 cgatcgtgaa ggagaataac aatgttcagg tcactgtttc tggcggccgc cctgatggca
    34201 tttaccccgc ttgcagcaaa tgcaggtgaa atcaccctac tgccatcgat caaattacaa
    34261 attggcgatc gcgatcatta cggtaattac tgggacggtg gtcactggcg agatcgtgat
    34321 tactggcatc gcaattatga atggcgcaaa aaccgctggt ggcgtcatga taatggctat
    34381 catcgtggct gggataagcg taaagcatat gagcgtggct atcgtgaagg ctggcgcgat
    34441 cgtgacgatc atcgcggaaa aggccgcgga catgggcacc gccattaaac gcgtcctcca
    34501 atggagcaca atgcctgatg cgacgctagc gcgtcttatc aggcctacaa accggctctt
    34561 taacgcgtag ggcggataag gcgtttacgc cgcatccgcc aatagtgcca gatgcgacgc
    34621 tcattcaact acaatcccag cgccgtcccc accaacaacc agatattcag cgccacgacc
    34681 agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag atcgcccatc
    34741 aacttgctgt cactggtgaa aatcagcagt ggaaccagcg ccagggcgat accaaaactt
    34801 aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag aatgacaata
    34861 aatgacggca acatggtgac tgtacgacgc acccacagcg ggatatggaa gcgaatgaat
    34921 ccctgcatca ccacctgccc cgccagtgtc cccaccaccg ttgaggacag tccggcagca
    34981 accagactta acccaaagac cgttgccgca gcatggctta acagcggttg cagcgtcaga
    35041 taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc cgccgcagct
    35101 gtagccatca tcaccagatt gacaaaaccg gcaatcgtca tggcgatagc cacatcccat
    35161 ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt gagcgaagag
    35221 tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc caggaagacc
    35281 gcctccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc caggttaggc
    35341 tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa cccgccaatc
    35401 actttctcca gcggtttttg cccgcgacgt tgcagcatta aaatcaggaa agtcgcgatc
    35461 cccgtcagca ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa accgatcgcc
    35521 gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg aacccaatag
    35581 aaccacacta cgggacgcgg atagtgatca cgaatctgct ccgccagatt tttaccggtg
    35641 gcaatcccta gtttggcaga gaggatctga atcagcatcg ccatcaggtt ggcccaaacg
    35701 acaacccaca gtagctgata gccgaagcta gcacccgcct gaatattggt cgcaaagtta
    35761 ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc gagcctcatc
    35821 ttacgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt gtgcctctaa
    35881 aacatagcct ttgctatgtt tcatgctatg ctaaacgaga atgattatca aattcattta
    35941 aatggattgt ggtgatttct ctgatagacc aagattatga ttacgaaaag attgatgact
    36001 tgaatgttat ggttatgttt aatgttagca catttacata actttcagct tccatacaca
    36061 acatagcaga aatgtatgac agatcactat ttttgaagcc tgtcacagga cgtcattata
    36121 gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg gaaatttaac
    36181 ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg cacttgccgt
    36241 tgttgtgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta tccgttatgt
    36301 tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact ccgacgttgg
    36361 tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcggat ttgccaacga
    36421 agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct tcttcctgaa
    36481 agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc acattcgcgt
    36541 gttgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg gtatgggcaa
    36601 actggaatcc tttaacgccg tcagctccct gattctgggt cagtcagaaa actttattgc
    36661 ctataaagat atcctcggca aaatctcccg taatcgtatg tacaccatgg ctgccacggc
    36721 aatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg aaccgaaata
    36781 cgtcgttgct gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt cgctgatcaa
    36841 tccttaccgt gttgatgcca gtgaagaaaa catccagatg tccaacctgc acgaaggtca
    36901 gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg ccattatcgt
    36961 tgccgcgatg ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac tgtttgccac
    37021 cgttactggc tggtttggct acagcatctc cttccagggc atcctgggct acatcttcta
    37081 tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg gcagtatcat
    37141 ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga aaattgcttc
    37201 cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctagtttcct tcgctaactt
    37261 ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc aaggtaacgt
    37321 ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg tgctgtctgc
    37381 gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa agccggggat cattccccgg
    37441 cttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata cccttgcaga
    37501 tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac aaactccgcg
    37561 accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat catcgcatcc
    37621 agcgtattcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat atcagcctgc
    37681 aaacgcttta accgttcgta gttggcatat ccggtgccaa agtcatcaat cgcaatccgg
    37741 aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt agaaaacgcc
    37801 tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg cttaaacaga
    37861 cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa attgactgaa
    37921 aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa ggattccagc
    37981 acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag aaacttatcc
    38041 ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata accttcaccc
    38101 tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa cgccgtgcga
    38161 atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc ctctctgcta
    38221 tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct taactgcccc
    38281 aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc accatagccc
    38341 atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt aaccatatgc
    38401 tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact acccggcaat
    38461 tgatacaact tttcgttttc ctgcatcaac ggcagcagcg tacggcaaat tgagcggata
    38521 cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt atcaatgcgc
    38581 aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc acgaaagttg
    38641 ggtaggagcg ttaacggatc tgttagcgcc tgcaaatgcc agcggcgatt aagccattca
    38701 ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat cagcaccgcg
    38761 agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg attgtaattc
    38821 agaaggcaaa gcgttgaaac agcccaggtg agatttaaaa acggatagcg aagattaccg
    38881 accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat aaagtcgttt
    38941 tcataaggtg tgcacaacag aagtagcagc acgcttaacg ctgccagcca gcttaaggta
    39001 aatgcgagtg agatagtaga aaagcatgtt gtaaatcagc actgcggtga aaaggcttag
    39061 taaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    39121 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    39181 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    39241 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctgccgag
    39301 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    39361 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    39421 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    39481 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    39541 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    39601 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca ggggccagcc tgttgccgat
    39661 ttttcatcca atgatgcttt caccaggcgg cgctaacccg gacgtagact gtattacaaa
    39721 agcggcaaaa agcagagaca aaaaaccccc gctttgcagc gagggttgga aatttggtgg
    39781 agctaagcgg gatcgaaccg ctgacctctt gcatgccatg caagcgctct cccagctgag
    39841 ctatagcccc acgatgcgtt tacgtaccaa atttgctggg tgcaaaattt ggtggagcta
    39901 agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc gctctcccag ctgagctata
    39961 gccccgtcac gtaaagcttg tcgagttgac gggcggcatc atatgaattc cgcccgaatg
    40021 tgtcaacggc aaattgcaac gtgtagtttc aatcgctgaa aaatcaggca aatgaacaat
    40081 tttggaaaac cgctcgcatt cagtagttat tcatgtcacg gtttcctgta aagtggtgtt
    40141 ataaaatgaa ctactaatag acccacatac attcagggaa ttgttatgtt caaggagagg
    40201 atgacaccag atgaacttgc cagactgacc ggctatagcc gccagaccat taataaatgg
    40261 gtacgcaagg aaggctggac aacgtcacca aaacctggcg tccagggtgg caaagcccga
    40321 ctggttcacg tcaatgaaca ggttcgtgaa tatattcgca atgctgaacg tccagaaggt
    40381 cagggagaag cgcctgccct ttctggtgat gcgccgcttg aagtattgct ggtgacactg
    40441 gcgaaagaga tgacgccagt tgaacaaaaa cagtttacat ccttgcttct gcgggaaggg
    40501 attatcggat tgttacaacg cttagggatt cgcgatagca aataatatga aaagattacg
    40561 caataaaatg accaccgaag aactggctga atgcctcggt gtggctaaac aaactgttaa
    40621 ccgttggacc agagaaaaag gctggaaaac ggaaaaattt cccggcgtga aaggcggtcg
    40681 tgccagactt attctggtcg atacgcaagt ttgcgagttt attcagaaca cgccagcctt
    40741 ccataacaca ccaatgttga tggaagctga agagagaatt gctgaatacg ctcctggagc
    40801 acgcgccccg gcgtatcgac aaatcatcaa cgcgatagat aatatgacag acattgagca
    40861 ggaaaaagtc gcgcaatttt tgtcacgtga aggaattcgc aatttcctcg cccgtctcga
    40921 tattgacgaa tcagcataaa taaaaaacgg caggatatta tctcctgccg tttatctttt
    40981 tacacgctaa ttactgctga ttttcgcgtt cagcaataaa atccagcgct ttgttgatac
    41041 gctcgatact gcgggtctta ccaattgcgt gaacggtaac atccagtgct ggagactgcc
    41101 ccgcaccggt tacggcgaca cgcagcggca taccaacttt acccataccc acttccagct
    41161 catccgccgt cgcctgaata gcgtgatgaa cgttttcagc ggtccagtca gtaatcgcgg
    41221 ccagtttgtc acgaaccact tccagcggct gacgcgctac cggacgcaga tgttttttcg
    41281 ccgcatcggc atcgaactca gcaaaatctt cgtagaaata acggcagctc tgtgccatct
    41341 ctttcagcgt cttgcagcgt tcgcccagca gtttcaccag atcagccagc tgcgggccgt
    41401 tacgggtatc gatattttcc tgctcaatgt gccactgtaa gtgagtagca acatactccg
    41461 gcggcagcgc gttaatgtag tgatggttca gccacagcag cttgtcggtg ttgaacgcac
    41521 tggcagattt gctgacggca ttcaaagtga agtatttgat catctcttca cgagtgaaga
    41581 tttcctgatc gccgtgggac cagcccagac gcaccagata gttcagcagt gcttctggca
    41641 aataaccgtc atcacgatac tgcattacgc tgactgcccc gtgacgtttg gacagttttt
    41701 taccgtcatc gccattgatc atagaaacgt gcgcgtaaac cggcaccggc gctttcaggg
    41761 ccttaagaat gttgatctgg cgcggcgtgt tgttgatatg gtcttcgcca cggataacgt
    41821 gggtgatttc catatcccag tcatcgacaa ccacacagaa gttataggtt ggggaaccat
    41881 cggtacggcg gataataaga tcgtccagtt cctggttgct gaactcgatc ggaccacgga
    41941 tctgatcgtc aaaaacaaca gaaccttcct gcgggttagc aaaacgtaca acacacggtt
    42001 catcatcagc atgatgctca tggctgtggc ggcagcgacc gtcataacgc ggcttctcac
    42061 ctttcgccat ttgctcttcg cgcagcgctt ccaggcgctc tttagagcaa tagcatttat
    42121 aagcagtgcc ctcttccagc atctgatcga tcaccgcgtt gtagcgatca aaacgtttgg
    42181 tctggtagta cggaccttca tcccactcca ggctcagcca gttcatgcca tccataatgg
    42241 cttcgatagc ttccggcgtg gaacgctcaa gatcggtgtc ttcaatacgc agcacgaact
    42301 caccgccgtg gttacgtgca aaaagccagg agtaaagagc agtacgcgcg ccgccaacgt
    42361 gcagatagcc tgttgggctt ggcgcgaagc gagttttgat tttcatgaaa tggccttacg
    42421 tttagaaaga tgccgacaac cggcaaatcc tggaaaaatt gagtgggcga tattctatca
    42481 ctcccgcctg attcctcaat gtagttcggg tttctacctt acgcattatt gttttcgttg
    42541 acaaattgcg caacacggtc tgttttgcga tcatcttgct taaatttacg acgaacgaac
    42601 aatttcttta gaaaatgcgt tgactcattt tgaactctcc ctataatgcg actccacaca
    42661 gcg
//
LOCUS       DAJTYM010000025        41272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTYM010000025 DAJTYM010000000
VERSION     DAJTYM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="OO575_001364"
     CDS             complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="OO575_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6664387.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1474..2898
                     /gene="miaB"
                     /locus_tag="OO575_001365"
     CDS             1474..2898
                     /gene="miaB"
                     /locus_tag="OO575_001365"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6664388.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3051..4091
                     /locus_tag="OO575_001366"
     CDS             3051..4091
                     /locus_tag="OO575_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6664389.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4088..4555
                     /gene="ybeY"
                     /locus_tag="OO575_001367"
     CDS             4088..4555
                     /gene="ybeY"
                     /locus_tag="OO575_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6664390.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4645..5523
                     /gene="corC"
                     /locus_tag="OO575_001368"
     CDS             4645..5523
                     /gene="corC"
                     /locus_tag="OO575_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6664391.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5548..7086
                     /gene="lnt"
                     /locus_tag="OO575_001369"
     CDS             5548..7086
                     /gene="lnt"
                     /locus_tag="OO575_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6664392.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7483..8391
                     /gene="gltI"
                     /locus_tag="OO575_001370"
     CDS             7483..8391
                     /gene="gltI"
                     /locus_tag="OO575_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6664393.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8561..9301
                     /gene="gltJ"
                     /locus_tag="OO575_001371"
     CDS             8561..9301
                     /gene="gltJ"
                     /locus_tag="OO575_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6664394.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9301..9975
                     /gene="gltK"
                     /locus_tag="OO575_001372"
     CDS             9301..9975
                     /gene="gltK"
                     /locus_tag="OO575_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6664395.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            9975..10699
                     /gene="gltL"
                     /locus_tag="OO575_001373"
                     /pseudo
     CDS             9975..10699
                     /gene="gltL"
                     /locus_tag="OO575_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
     gene            10817..11752
                     /gene="rihA"
                     /locus_tag="OO575_001374"
     CDS             10817..11752
                     /gene="rihA"
                     /locus_tag="OO575_001374"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6664396.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11836..13506
                     /gene="hscC"
                     /locus_tag="OO575_001375"
                     /pseudo
     CDS             11836..13506
                     /gene="hscC"
                     /locus_tag="OO575_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13566..15017)
                     /gene="djlC"
                     /locus_tag="OO575_001376"
     CDS             complement(13566..15017)
                     /gene="djlC"
                     /locus_tag="OO575_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6664397.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15014..15721)
                     /locus_tag="OO575_001377"
     CDS             complement(15014..15721)
                     /locus_tag="OO575_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6664398.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15823..16377
                     /locus_tag="OO575_001378"
     CDS             15823..16377
                     /locus_tag="OO575_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6664399.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16387..17814)
                     /locus_tag="OO575_001379"
     CDS             complement(16387..17814)
                     /locus_tag="OO575_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6664400.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17811..18518)
                     /locus_tag="OO575_001380"
     CDS             complement(17811..18518)
                     /locus_tag="OO575_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6664401.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18682..19659
                     /locus_tag="OO575_001381"
     CDS             18682..19659
                     /locus_tag="OO575_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6664402.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19729..20211)
                     /locus_tag="OO575_001382"
     CDS             complement(19729..20211)
                     /locus_tag="OO575_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6664403.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20446..23028
                     /gene="leuS"
                     /locus_tag="OO575_001383"
     CDS             20446..23028
                     /gene="leuS"
                     /locus_tag="OO575_001383"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6664404.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23043..23624
                     /gene="lptE"
                     /locus_tag="OO575_001384"
     CDS             23043..23624
                     /gene="lptE"
                     /locus_tag="OO575_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6664405.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23624..24655
                     /gene="holA"
                     /locus_tag="OO575_001385"
     CDS             23624..24655
                     /gene="holA"
                     /locus_tag="OO575_001385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6664406.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24657..25298
                     /gene="nadD"
                     /locus_tag="OO575_001386"
     CDS             24657..25298
                     /gene="nadD"
                     /locus_tag="OO575_001386"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6664407.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25322..25933
                     /locus_tag="OO575_001387"
     CDS             25322..25933
                     /locus_tag="OO575_001387"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6664408.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26193..26510
                     /gene="rsfS"
                     /locus_tag="OO575_001388"
                     /gene_synonym="rsfA"
     CDS             26193..26510
                     /gene="rsfS"
                     /locus_tag="OO575_001388"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6664409.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26514..26981
                     /gene="rlmH"
                     /locus_tag="OO575_001389"
     CDS             26514..26981
                     /gene="rlmH"
                     /locus_tag="OO575_001389"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6664410.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27012..28913
                     /gene="mrdA"
                     /locus_tag="OO575_001390"
     CDS             27012..28913
                     /gene="mrdA"
                     /locus_tag="OO575_001390"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6664411.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            28916..30028
                     /gene="mrdB"
                     /locus_tag="OO575_001391"
                     /gene_synonym="rodA"
     CDS             28916..30028
                     /gene="mrdB"
                     /locus_tag="OO575_001391"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6664412.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30039..31127
                     /gene="rlpA"
                     /locus_tag="OO575_001392"
     CDS             30039..31127
                     /gene="rlpA"
                     /locus_tag="OO575_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6664413.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31266..32477
                     /gene="dacA"
                     /locus_tag="OO575_001393"
     CDS             31266..32477
                     /gene="dacA"
                     /locus_tag="OO575_001393"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6664414.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32588..32851
                     /locus_tag="OO575_001394"
     CDS             32588..32851
                     /locus_tag="OO575_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6664415.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            32952..33593
                     /gene="lipB"
                     /locus_tag="OO575_001395"
     CDS             32952..33593
                     /gene="lipB"
                     /locus_tag="OO575_001395"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6664416.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33852..34805
                     /locus_tag="OO575_001396"
     CDS             33852..34805
                     /locus_tag="OO575_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6664417.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35014..35979
                     /gene="lipA"
                     /locus_tag="OO575_001397"
     CDS             35014..35979
                     /gene="lipA"
                     /locus_tag="OO575_001397"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6664418.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36080..36283)
                     /gene="tatE"
                     /locus_tag="OO575_001398"
     CDS             complement(36080..36283)
                     /gene="tatE"
                     /locus_tag="OO575_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6664419.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36412..37200)
                     /locus_tag="OO575_001399"
     CDS             complement(36412..37200)
                     /locus_tag="OO575_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6664420.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37293..37676
                     /gene="crcB"
                     /locus_tag="OO575_001400"
     CDS             37293..37676
                     /gene="crcB"
                     /locus_tag="OO575_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6664421.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37730..37939)
                     /gene="cspE"
                     /locus_tag="OO575_001401"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37730..37939)
                     /gene="cspE"
                     /locus_tag="OO575_001401"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6664422.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38114..38674)
                     /gene="pagP"
                     /locus_tag="OO575_001402"
     CDS             complement(38114..38674)
                     /gene="pagP"
                     /locus_tag="OO575_001402"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6664423.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39263..40648
                     /gene="dcuC"
                     /locus_tag="OO575_001403"
     CDS             39263..40648
                     /gene="dcuC"
                     /locus_tag="OO575_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6664424.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40689..41030)
                     /locus_tag="OO575_001404"
                     /pseudo
     CDS             complement(40689..41030)
                     /locus_tag="OO575_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT        10355 a        10405 c        10286 g        10226 t
ORIGIN      
        1 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc
       61 gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct
      121 acggctggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
      181 tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc
      241 agcggcggca gattattact gaatccggca taaaacagat ccataccact ttgcataatg
      301 aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct
      361 tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca
      421 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
      481 agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca
      541 agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc
      601 gccatattca tattctgcaa ctggcgaata cgcgtcggag agtcatacca caccagcgat
      661 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
      721 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac
      781 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca
      841 ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca
      901 ttatctcgat gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct
      961 tccagcgcct gccacaacgc ctgttgcagg actgtgtttt ccaccatgta gccaagcaac
     1021 ggcaacttta attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc
     1081 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
     1141 agcaatgata ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg
     1201 acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc
     1261 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
     1321 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
     1381 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
     1441 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
     1501 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
     1561 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc
     1621 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
     1681 aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc
     1741 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
     1801 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
     1861 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg
     1921 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
     1981 cgtggtgaag aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg
     2041 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
     2101 aactacgacg gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac
     2161 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
     2221 gaagtgtacc gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt
     2281 tccgatcgca ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc
     2341 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc
     2401 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
     2461 ttcgacatga gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg
     2521 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc
     2581 aatcagcaag cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta
     2641 gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg
     2701 gtcaacttcg agggcacacc tgatatgatc ggtaaattcg tcgatgtaga aattaccgac
     2761 gtctacccga actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc
     2821 atggcagaaa caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg
     2881 ggttattatc agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct
     2941 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
     3001 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
     3061 acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc
     3121 cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
     3181 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
     3241 gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac
     3301 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
     3361 agtacggcaa agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa
     3421 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
     3481 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
     3541 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
     3601 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt
     3661 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
     3721 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
     3781 gccagaacac taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
     3841 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct
     3901 tacgtcatgc cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
     3961 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg
     4021 aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
     4081 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
     4141 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
     4201 gtcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
     4261 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat
     4321 ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
     4381 ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca
     4441 tttgttaggt tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac
     4501 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
     4561 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
     4621 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
     4681 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
     4741 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
     4801 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
     4861 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
     4921 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
     4981 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
     5041 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
     5101 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc
     5161 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
     5221 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg
     5281 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
     5341 gatgaagaag tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg
     5401 cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg
     5461 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa
     5521 taaaaccgaa actggataga taactacatg gattttgcct cattaattga acgccagcgc
     5581 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
     5641 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt
     5701 cgcccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt
     5761 attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc
     5821 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
     5881 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc
     5941 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
     6001 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc
     6061 attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac
     6121 tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc
     6181 caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt
     6241 ccgcaatcgc tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac
     6301 gcaacggcac cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
     6361 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa
     6421 ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat
     6481 acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat
     6541 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
     6601 cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
     6661 cagccgccgc tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagattatt
     6721 ctcgccgagc aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc
     6781 aacgatgcgt ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg
     6841 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
     6901 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc
     6961 actaacgtga cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg
     7021 tgggtgctga cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt
     7081 aaataatccc ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc
     7141 ggcatcagtg cattatccgg ataacaatat tctcctatcc aactttggca catctattgc
     7201 tttgttatac aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat
     7261 ccaccggtag caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac
     7321 ctcgcaaaaa caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc
     7381 taacaggcac aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg
     7441 ggtatccatg cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct
     7501 gccacagcaa tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
     7561 ccggcagcag gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
     7621 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg
     7681 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
     7741 caggtaaaac tgattccgat tacctcacaa aatcgtattc cactgctgca aaacggcact
     7801 ttcgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc
     7861 tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa
     7921 gattttgccg acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt
     7981 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
     8041 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac
     8101 gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc
     8161 aagccgcagt ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa
     8221 aagctgatgg atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
     8281 gataaatggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
     8341 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa
     8401 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
     8461 atggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
     8521 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
     8581 gggtattttt ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag
     8641 tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
     8701 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
     8761 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt
     8821 gatcccggag ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa
     8881 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
     8941 cgaacaggta cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc
     9001 gatgggcctg acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt
     9061 tatcgtcccg ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc
     9121 gaccatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca
     9181 cgcctgggag tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat
     9241 gctggtgatg acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata
     9301 atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg
     9361 gtgatcaccc tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg
     9421 ctggcggtga tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt
     9481 aacgtattcc gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg
     9541 ggatttctgc aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg
     9601 atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt
     9661 caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag
     9721 tcgatgaaac tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact
     9781 cagggcatcg tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc
     9841 ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt
     9901 gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa
     9961 agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    10021 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt
    10081 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag
    10141 gtgaaatcac cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc
    10201 gctcccgcgt cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg
    10261 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa
    10321 agcattgaaa ttgctggagc gcgtgggact ttccgcacat gccaataaat ttccggcgca
    10381 gctttccggc ggtcagcagc agcgcgtggc aatcgctcgc gcattgtgta tggctcctat
    10441 tgcgatgctg ttcgacgaac cgacatcggc gctggatccg gagatgatca acgaagtgct
    10501 ggacgtgatg gtggaactgg cgaacgaagg aatgaccatg atggtggtga cccacgaaat
    10561 gggctttgcc cgcaaagtgg cgaatcgggt gatctttatg gacgagggta aaattgtcga
    10621 agactcgccg aaagacgctt tcttcgatga tccgaaatcg gaccgcgcaa aagacttcct
    10681 cgcgaaaatc ctgcattaat cactctggcg cacataccca attgcgcgcc atctcacgct
    10741 ttatcgtttc gcccctcgta tctgttacat cctggcgtta cccttgtcgc aaagaagcac
    10801 acaacaagga gcaacaatgg cactgccaat tctgttagat tgcgacccag gtcatgatga
    10861 cgctatcgca atagttctcg cactcgcctc accagagctt gatgtcaaag caattacgtc
    10921 ttccgccgga aaccagacac cagaaaaaac cttacgcaat gttctgcgta tgctgacctt
    10981 gcttaatcgc accgatattc cggtggcagg cggcgcggta aaaccgttaa tgcgtgagtt
    11041 gattatcgcg gacaatgtgc acggcgaaag cggtctcgac ggcccggcat taccggaacc
    11101 gacgttcgca ccgcaaaact gtacggcggt ggagctgatg gcgaaaacgc tgcgcgaaag
    11161 tgcggaacct gtcacgattg tttctaccgg accgcaaact aacgttgcct tgctgctcaa
    11221 tagccatccg gaactgcata gcaaaattgc ccgtatcgtg attatgggcg gcgcaatggg
    11281 gcttggtaac tggacgcctg cggctgaatt taatatttac gttgacccgg aagcggcaga
    11341 aattgtcttc cagtcaggga tcccggtggt gatggccggt ctggatgtta ctcataaagc
    11401 gcaaatccac gttgaagaca ccgagcgttt ccgcgcgatt ggcaaccctg tttcaaccat
    11461 tgttgccgaa ctgctggatt tcttcctcga atatcataaa gacgaaaaat ggggctttgt
    11521 cggcgcaccg ctgcatgacc catgcaccat cgcctggctg ttgaaacccg agttgtttac
    11581 cactgttgag cgctgggttg gcgtggaaac acaggggaaa tatacccagg gtatgacggt
    11641 tgttgattat tattatctga ctggcaataa accgaatgcc actgtaattg tcgatgttga
    11701 tcgtcagggc tttgttgatt tactggctga tcgtctgaaa ttttacgctt aacactcgtc
    11761 attgattaag atcaccacgc gttacctggg tggtcttaat ctatttatct ctttttcttt
    11821 caagatataa atgcaatgga taatgcagaa ctcgccatag gtatcgatct cggcactacc
    11881 aatagtttaa ttgccgtctg gaaagacggt gccgcgcaat taattccaaa taagttcggt
    11941 gaatatttaa caccatccat aattagcatg gatgaaaata atcatatttt agtcggaaaa
    12001 ccggctgtat cacggcgtac ttcgcatccg gataaaacgg cagcgttatt taaacgtgca
    12061 atgggcagta ataccaactg gcggttaggc agcgacacat ttaacgcgcc agaactgtcc
    12121 tctttggtat tacgctcatt aaaagaagat gccgaagaat ttctgcaacg tccgattaaa
    12181 gatgtggtga tctccgttct ggcttatttc agcgatgaac aacgcaagca tacccgttta
    12241 gcagcggact tagccgggtt aaatgcggta cgcttaatta atgaacccac agcagctgcg
    12301 atggcgtatg gcctgcatac ccaacaaaat acccgttcgc tggtgtttga tctcggtggc
    12361 ggcacgtttg acgttacggt gcttgagtac gccacgccgg tgattgaagt tcacgcctcc
    12421 gctggcgaca acttcctcgg tggcgaagat tttacccata tgttggtcga tgacgtttta
    12481 aagcgcgcgg atgtcacaag gaccacgctt aacgagagtg aactggcagc cttgtacgcc
    12541 tgtgtggaag cggcaaaatg tagcaatcaa tcgccaatgc acattcgctg gtagtaccag
    12601 gatgaaacgc gggaatgcga attttacgag aacgaactgg aagatttgtg gttgccgctg
    12661 ctcaatcgct tgcgagtgcc gattgaacag gcgttgcgcg atgcgcgtct gaagccgagt
    12721 caaatcgaca gtctggtgct ggttggcggc gcgtcacaaa tgccgctggt gcagcgaatc
    12781 gccgtgcgtc tgtttggcaa attaccgtat caaagttacg atccgagcac cattgtcgcg
    12841 ctgggcgcag caatccaggc cgcctgccgc ttacgcagtg aagatattga agaagtgatc
    12901 ctcactgata tttgccctta ctcgttgggc gttgaggtta accgccaggg cgtttccggc
    12961 attttctcgc cgattattga acgaaacacc actgtgcccg tgtcgcgtgt agaaacttat
    13021 tcaaccatgc acccggaaca ggattcaatt acggttaacg tctatcaggg agaaaaccac
    13081 aaagttaaaa acaacattct ggtggaatcc ttcgatgtgc cgttgaagaa aaccggggct
    13141 tatcagtcga ttgatattcg ctttagttat gatatcaacg ggttgcttga agttgacgtg
    13201 cttctggaag acggcagcgt taagtccaga gtgattaacc acagcccggt aacattgagc
    13261 gcgcagcaga ttgaagagag tcggacgcgg ttatccgcat tgaaaattta tccgcgcgat
    13321 atgctcatca atcgcacctt taaagccaaa ctggaagagt tgtgggcgcg ggcgctgggt
    13381 gacgagcgag aagagattgg ccgggtgatc accgattttg atgcggcgct gcagtcaaac
    13441 gatatggccc gcgtcgatga ggtcaggcgg cgggcgagcg tttatttagc cattgagacc
    13501 tcataatcac ggctgccgga tgcggcgtaa atgctgcatc cggcatcggc gcaaaaaaca
    13561 catcgtcaac taaacaacat cgcgattttg ccgagagcat aacctataac cagcaccgtt
    13621 aaactgattt tgataatgct ggtaatgctc tgtggttttt caccctcatc atttttttct
    13681 tcatggtttt caggtgttgg ctcatcgaca tcttcatcct ccggctcgag ttttactttc
    13741 gtcaacagca tccgggtgat aatggcctct aatggatgag catctccgcc ttccagcctt
    13801 gtattaagac gggccagcat ctcttgtttc ggaaacgctt caagtactac ctgttccgct
    13861 acccgataca aatccagtaa cggtgaatct tcttctgtct cgctaccgac tctcgatttc
    13921 caccacgccc atgctttgcg tttgaagaat aacgactcaa taaacgattg acctaacggc
    13981 gataattttc cgctatgtaa aatttccgcc cataacagcc gcgtccgggc gctctcacca
    14041 ggaacatatt tcagcggttg accttgcgca tctaccatgc tccgcgcttc gataaccatc
    14101 actaataacg cgaaataatc cgggcaatga cgcttgctcc actgcaacaa caaattatca
    14161 gcttgtgtag atgggtaact ttcccagtac gcgccgagat cagcaagcag gctttccccc
    14221 tcgccgcagt acaaacgttg tgcacactgg taataccggg catcttcgct ttctggttct
    14281 tccgcttgcc actgtttggc tacaggaatg agttccacca tacctgtttg caccgagctg
    14341 taccagcgca acagtttgcg atgtaacttt gcatcatcag gaatgaacca cggtccttcc
    14401 atcgccaaaa acgcagtgac atagtcagga tggtaacggc agcaacgttc cagcgcgaag
    14461 taatattcaa cagtctggtt ctgtaacgcg acgggatatt ccagaaggct aaggaaatcg
    14521 aaacaatcac cagcctgaat ggcctcaaga aaagcctccc tttcttcctc atccattcct
    14581 tcgctgtcat taaacgaatg ccagttcaaa cgctctgcca acaaagaagc acaatttaat
    14641 gacagatatc gcgcttctat agcgattgca cacagcggcc agcgtaattg atcgacatcc
    14701 tccatgttcc aggtatttaa ttgctgaata aatttctgcc aggcggaggg ctgaaaacga
    14761 tcactttctg aatccagtaa tgtcctgaat gcacgtagta tttcatgttc agcggtggca
    14821 tctttttctt catcatctgc ttcctcgaca ggatttaccg ccagtcgcag cgcctcttca
    14881 taggcctggc gtaatgcttt aaacccctgc ggatcggttt cgggatgaca caacggtaag
    14941 cgagcaagat aagcctggcg gataatgtct atttgcgtcg tgctttcaat ttcgaggatt
    15001 tgccaacatg ttttcatagg tcgctcacat ccttaagcgt taccgggcat tcaggctgga
    15061 cagttaatgg ctgccaggga agcgtggcat agaaacggtc gcttcccgct gccagctctt
    15121 cgaacatccg actcccggcg ctatcgtatt tgtacagggt tagcgcctcg cgtagcgaag
    15181 cgttgtcttc gcactgagaa gattgccagt agagacgccc tagtgaatag gcagaaaaat
    15241 agtgcatcca gctgtgatag taatggtgag ctcgtgcata gacgcgcgat tgcagccata
    15301 aactctcttc ttccgtcagc cagttgttga gcacaccggc ccgtgacaga aatcccattc
    15361 gaacataatc ccaggctttg attccccctt ctccacagca catagcggtt tgggcaacga
    15421 attgggtata aatcaaccct ctctccgagc caccagcaat taatgctttc cactcttcag
    15481 gggaagagcg ttgccatgtc agatataagc cagctaaatc attggcatga ccatcatcag
    15541 tcatctgata aatagtctga attaaatcat cacgattatt tattccccag gatgatgaga
    15601 gatcgaattc ttttatcccg gtatacattt ttggcgagtc atgcgcgggt ttgcaactct
    15661 gattaagtat ttccatgggc gcagaaagcg caaaaagaag atattgttct tccttattca
    15721 ttttttaata atcccttaag tgcattaact gactactttt ttattattga tgagtataaa
    15781 cttgacgctt tcaaaataaa aagaaaatcg aagcattcac acatgaataa aaaattaatg
    15841 tatatattcg caatttttat agttgcagca attacctgta ttagccaacc caagaaaacg
    15901 atgttgcgtg ataaagccat ggtgaattat gcctttgatt atttaagatc accaggcagt
    15961 ctaccgttaa ccacggcagc cacggagctt tccgcgattc atggtcactc aacatcgcaa
    16021 tatcgccttg gcgaatttta tcttcatggt agcgacggta aaccactgga ttatacacag
    16081 gcgagatact ggtatgagca atcagcagaa caggaaaatc cgcgcgcaca aagcaaactg
    16141 gggtggatct acctgaaagg cctgggagtc aaacccgata cccgtaaagc aattctctgg
    16201 tataaggaag cagctgaaca agggtatgct catgctcaat atactttggg tttgatctac
    16261 agaaatggca ctggtattaa tgttaaccat tatgaatctc aaaaatggtt aaaactggcc
    16321 gccaaacaac attacaaaaa tgcggaaaga ttacttgccg gacttcccgc acattaacgt
    16381 cgactgtcac ccgaagagat ggagtatttt ccctatcaaa ccggcaaaga taaaaatgta
    16441 gaaaataatt ttgatgacgc ccagacagcc cagcccgcca tcatcaacgg gcattggctg
    16501 cggttgcggc tccggctcga cggtttcctg accggtaaaa ttcgccgcct gaattaataa
    16561 tcgacgaata attaatgctt ccagtggaca acgatcttct gcatcaaggc ggctcacaat
    16621 cggcattaat gattcttctg tcggttggct ggcctgcact aattgtttgg caactaaata
    16681 taatggccat aagggatgtt ctgtatcacg cgtgagtgag atccgcggct tacgtccttt
    16741 gtgcaaaacc atctcgacca gtgaacgccc cagcgcagag agtttttcat cgctgagtat
    16801 ttcgtataaa tgaaaacgcg tacgggcgct gtcgccgggg acataaagta gcggttttcc
    16861 ctgatcgtta accagatcac gcgcttcaat catcatcacc actaatgggt aataatagac
    16921 ccgatgctga cggcaccatt gcaacattaa agcatccgcc tgggtggagg gatactcgcg
    16981 ccagtagtaa caaagttctg ccagcaggct ttcgccttct ccacaataga cgcgttgtgc
    17041 gtattcataa taacgcgcat cttcattatc aggttcagct tcccgccacg accgggcgta
    17101 atccaggagt tcggggattc ccaggcggct tgcgctatac cagcggagta aatttctgtg
    17161 cagttttgca tcatcgggga taatcactgc gcgatgttgc gctaaataca atgtcagcca
    17221 ttctggatgc gatgaccaaa tccgagcgaa catttggtaa aaatcgatgg tgtcattttg
    17281 cacggcaact ggcagatgca gaatggtctg gaagttaaaa acattcccct tcgcaatggc
    17341 atataaaaag gattccagtt cttcttcgtc tatttccccg acgttttcct cctgcaacca
    17401 ccgcaatctt tctgctaata acaccacgca ctcgaaggat aaatgggcag tgttcatggc
    17461 gattgtgcac agcgaccagc gtaattcatc aatctcttcc atcgagcaat aatttaattg
    17521 ctgaatgaat cgctgccagg cggagggcag aaaacgttcg ctatcagagg caagtaacgc
    17581 gcgaaaggcg agcagaattt catgttctgc tacctcatat tcttctggtt gccaaacaga
    17641 tttagcaggc gactgcgcaa tccgtagcgc ctcctcatac gcctgacgaa gttgtttaaa
    17701 accctgcgga tcggtttctg gatgaaagga cggtaacagc gccagataag cgcggcggat
    17761 aatatcgacg tcagtcgttt cctctatatc gaggatcttc cagcaatttt tcataagtcg
    17821 ctcatatcct taagcgtttc cgggcatgtg gggtagtcag ccagtggttg ccaggacaag
    17881 gtagggtaat acgcatcatc actggcaaat aattgataaa acatatcatt gccggaatcg
    17941 tcgtattctt tacgcgctaa gaattcgcgt aataaaggca gatgatcatc ttcgggagac
    18001 tgccaatatt gccgaccaaa ggtataaccg gcaaagtatt gccgccagtt actataataa
    18061 cgtagagccc gtagatgaat acgcgactgg atccatagac tttcttcttc actcaaccag
    18121 ttattaagta cgcccattcg acttaaaaaa cccatccgga cataatccca tgctttaatt
    18181 cctccctcac cacagcattc ggctgtgctg gcgacaaatt gcgcataagc ctgaccttgc
    18241 tcatttaatt cagcaagata gtcacgccat tcacatggag aataacgaaa ccagcggtga
    18301 taaaaccctg ccaaccgagc ggcatgaccg ttctcgatca ttcggtgaat tatgcgcagc
    18361 aggtcatcac ggtcatcaat tccccatgag gaagaaaggt caaaaaagca tgtacctaaa
    18421 tacattttgg gggatgaatg cgaaggtaaa cagcattcat tgcgtatttc catcggagtg
    18481 gaaagtgcaa acaatatttt ttgcgacccc atgtccatat aatttatcct taaacattca
    18541 tttaatgaat gtaaactaca tttcgaggct cagttattta tacttttctg acgcaacata
    18601 atacgcataa aataaggctt acgcaaaaat ggaacacata aaaatataat atattttatc
    18661 tacaaccata aggaaatgat aatgattttt acgtcaagtt gctgcgataa tttatcaata
    18721 gatgagatta tcgaacgtgc tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt
    18781 tattataatc gcgatagcgc aattgactct ccggacgacg aaaaggcctt ttactggctg
    18841 aagctggccg ctgagcaagg tcattgtgaa gcacagtatt ccttagggca aaagtatacc
    18901 gaggataaaa gccgtcataa agataatgag caagccatct tctggctgaa aaaagctgcc
    18961 ctacaaggcc atactttcgc ttccaacgcc cttggctgga tactggatcg tggagaagac
    19021 cccaactata aagaagcggt tacctggtat cagatagccg cggagagcgg aatgtcttat
    19081 gcgcaaaata atcttggatg gatgtacaga aatggcaacg gagtcgcaca agactatacg
    19141 ctggcatttt tctggtacaa acaagctgca ttacaaggcc atagttacgc acaagacaat
    19201 ctggccgatc tttataaaga cggagaaggc gttgctcaaa acaagacact cgccgcattc
    19261 tggtatttga aaagcgcaca gcagggtaat cggcacgccc agtttcaaat tgcatgggat
    19321 tataacgctg gcgaaggggt ggaccaggac tataagcaag cgatgtactg gtatctgaag
    19381 gctgccgctc aggaaagcgt cggcgcttac gtcaacatcg gttatatgta taaacacgga
    19441 caaggcgttg agaaagatta tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc
    19501 aatgacgcca ctgcctggta taacctggcc attatgtatc actacggaga aggaagacct
    19561 gtcgatctcc gacaggctct cgacctgtat cgtaaagttc agtcatccgg aaccagagat
    19621 gtcagtcagg aaattcgtga gactgaagct ttactgtaga aataaaaaca agggccgggg
    19681 aatttcattc tgcccatatt ctctttattg ctgtgcgaaa tcagactatt acggctcaaa
    19741 cgggcgtctc tggaactggt cataaccaca tttcgggcat agcgtcagca cttccggtgt
    19801 gtagatcggg agatggaagt gacatttctc gcagaccaga tttcccagcc cgaccacttc
    19861 tccgctgtga taaaccccat gatgattgag gtcctggaaa atttcgcgcc attcaagctg
    19921 cgttttatcg gtgatgtctg ccagctcctg ccacaagctt tctttaatca cccgcataaa
    19981 gacgctgtca gattcttctt tcaggctctc ttcatagctc atggcgaact cttccaggtc
    20041 acgtctgaca gctcgcgtca gctcatcgat ctcggttcgc gttaactccc ctgtttttat
    20101 tacgcgctcg cgcgcctgtt ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg
    20161 ttcgttcagt gacgcgacca gttcacggta atattgagca accttgttca tcgtttcatc
    20221 tcccgggtcg ttaactgact ataataatag acgttctttg ttccttgctt tgctaatacg
    20281 cccacaaact ctgtaaatta atgtacgaat aatccgggca tcatttcaac gtcgagtgcg
    20341 cgaaaggctg ttttgacgcg gacgtttggg ctatgctatg cggatctgaa aaaccacctc
    20401 aacgctacat ttgtagccgt attgaaaaca ggaccactgg ctgccatgca agagcaatac
    20461 cgcccggaag agatagaatc caaagtacag cttcattggg atgagaagcg cacatttgaa
    20521 gtaaccgaag acgagagcaa agagaagtat tactgcctgt ctatgcttcc ctatccttct
    20581 ggtcgactac acatgggcca cgtacgtaac tacaccatcg gtgacgtgat cgcccgttac
    20641 cagcgcatgc tgggcaaaaa cgttctgcag ccgatcggct gggacgcgtt tggtctgcct
    20701 gcggaaggcg cggcggtgaa aaacaacacc gctccggcac cgtggacgta cgacaacatc
    20761 gcgtatatga aaaaccagct caaaatgctg ggctttggtt atgactggag ccgcgagctg
    20821 gcaacctgta cgccggaata ctaccgttgg gaacagaaat tcttcaccga gctgtataaa
    20881 aaaggcctgg tatataaaaa gacttctgcg gtcaactggt gcccgaacga ccagaccgta
    20941 ctggcgaacg aacaagttat cgacggctgc tgctggcgct gcgataccaa agttgaacgt
    21001 aaagagatcc cgcagtggtt tatcaaaatc actgcttacg ctgacgagct gctcaacgat
    21061 ctggataaac tggatcactg gccagacacc gttaaaacca tgcagcgtaa ctggatcggt
    21121 cgttccgaag gcgtggagat caccttcaac gttaacgact atgacaacac gctgaccgtt
    21181 tacactaccc gcccggacac ctttatgggt tgtacctacc tggcggtagc tgcgggtcat
    21241 ccgctggcgc agaaagcggc ggaaaataat cctgaactgg cggcctttat tgacgaatgc
    21301 cgtaacacca aagttgccga agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact
    21361 ggctttaaag ctgttcaccc attaacgggc gaagaaattc ccgtttgggc agcaaacttc
    21421 gtattgatgg agtacggcac gggcgcagtt atggcggttc cgggtcacga ccagcgcgac
    21481 tacgagtttg cctctaaata cggcctgaac atcaaaccgg ttatcctggc ggctgacggc
    21541 tctgagccag atctttctca gcaagccctg actgaaaaag gcgtgctgtt caactctggc
    21601 gagttcaacg gtcttgacca tgaagcggcc ttcaacgcca tcgccgataa actgactgcg
    21661 atgggcgttg gcgagcgtaa agtgaactac cgcctgcgcg actggggtgt ttctcgccag
    21721 cgttactggg gcgcgccgat tccgatggtg acgctggaag acggtaccgt aatgccaacc
    21781 ccggacgacc agctgccggt gatcctgccg gaagatgtgg tgatggacgg cattaccagc
    21841 ccgattaaag cagatccgga gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt
    21901 gaaaccgaca ctttcgacac ctttatggag tcctcctggt actatgcgcg ctacacttgc
    21961 ccagagtaca aagaaggtat gctggattcc gaagcggcta actactggct gccggtggat
    22021 atctacattg gtggtatcga acacgccatt atgcacctgc tctacttccg cttcttccac
    22081 aaactgatgc gtgatgcagg catggtgaac tctgacgaac cagcgaaaca gttgctgtgt
    22141 cagggtatgg tgctggcaga tgccttctac tatgttggcg aaaacggcga acgtaactgg
    22201 gtttccccgg ttgatgctat cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa
    22261 gatgcggcag gccatgaact ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac
    22321 aacggtatcg acccgcaggt gatggttgaa cgttacggcg cggacaccgt tcgtctgttt
    22381 atgatgtttg cttctccggc tgatatgact ctcgaatggc aggaatccgg tgtggaaggg
    22441 gctaaccgct tcctgaaacg tgtctggaaa ctggtgtacg agcacacagc aaaaggtgat
    22501 gttgcggcac tgaacgttga tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg
    22561 cataaaacta tcgctaaagt gaccgatgat atcggccgtc gtcagacctt caacaccgca
    22621 attgcggcga ttatggagct gatgaacaaa ctggcgaaag caccgaccga tggcgagcag
    22681 gatcgcgctc tgatgcagga agcactgcag gccgttgtcc gtatgctcaa cccgttcacc
    22741 ccgcacatct gcttcacgct gtggcaggaa ctcaaaggcg aaggcgatat cgacaacgcg
    22801 ccgtggccgg ttgctgacga aaaagcgatg gtggaagact ccacgctggt cgtggtgcag
    22861 gttaacggta aagtccgtgc caaaatcacc gtcccggtgg acgcaacgga agaacaggtt
    22921 cgcgaacgtg ctggccagga acatctggta gcaaaatatc ttgatggcgt tactgtacgt
    22981 aaagtgattt acgtaccagg taaactcctc aatctggtcg ttggctaagc gcgggaggaa
    23041 gcgtgcgata tctggcaaca ctgttgttat ctctggcggt gttaatcacc gccgggtgtg
    23101 gctggcatct gcgtgacacc acgcaggttc cttccactat gaaggtcatg atcctggact
    23161 caggcgatcc gaacgggcca ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg
    23221 tcgagttgct tgataaagaa accacgcgta aggacgttcc atccctgcgt ttgggtaaag
    23281 tgagcatcgc gaaagatacc gcatcggtat tccgtaacgg tcaaacagca gagtatcaga
    23341 tgatcatgac ggtgaatgcg accgtgttga tccccggccg tgatatctac ccgattagcg
    23401 ccaaagtctt ccgttcattc ttcgataacc cgcaaatggc gttagcgaaa gataacgaac
    23461 aagacatgat cgtaaaagag atgtacgacc gtgctgccga acagctgatt cgtaagctgc
    23521 caagcatccg tgctgcggat attcgttccg acgaagaaca gacgtcgacc acaacggata
    23581 ctccggcaac gcctgcacgc gtctccacca tgctgggtaa ctgatgattc ggttgtaccc
    23641 ggaacaactc cgcgcgcagc tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa
    23701 cgatcctctg ttattgcagg aaagccagga cgctgttcgc caggtagctg cggcacaagg
    23761 attcgaagaa caccacactt tttccattga tcccaacact gactggaatg cgatcttttc
    23821 gttatgccag gctatgagtc tgtttgccag tcgacaaacg ctattgctgt tgttaccaga
    23881 aaacggaccg aatgcggcga tcaatgaaca acttctcaca ctcaccggac ttctgcatga
    23941 cgacctgcta ttgatcgtcc gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg
    24001 gtttactgct cttgcgaatc gcagcgtgca ggtgacctgt cagacaccgg agcaggctca
    24061 gcttccccgc tgggttgctg tgcgcgcaaa acagctcaac ttagaactgg atgacgcggc
    24121 gaatcaggtg ctctgctact gttatgaagg taacctgctg gcgctggctc aggcactgga
    24181 gcgtttatcg ctgctctggc cagacggcaa actgacatta ccgcgcgttg aacaggcggt
    24241 gaatgatgcc gcgcatttca ccccttttca ttgggttgat gctttgttga tgggaaaaag
    24301 taagcgcgcg ttgcatattc ttcagcaact gcgtctggaa ggcagcgagc cggttatttt
    24361 gttgcgcaca ttacaacgtg aactgttgtt actggttaac ctgaaacgcc agtctgccca
    24421 tacgccactg cgtgcgttgt ttgataagca tcgggtatgg cagaaccgcc ggggcatgat
    24481 gggcgaggcg ttaaatcgct taagtcagcc gcagttacgt caggccgtgc aactcctgac
    24541 acgaacggaa ctcaccctca aacaagatta cggtcagtca gtgtgggcag agctggaagg
    24601 gttatctctt ctgttgtgcc ataaacccct ggcggacgta tttatcgacg gttgatatga
    24661 aatctttaca ggctctgttt ggcggcacct ttgatccggt gcactatggt catctaaaac
    24721 ccgtggaaac gctggcgaat ttgattggtc tgacgcgggt cacaatcatc cctaataatg
    24781 ttcctccgca tcgtccccag ccggaagcga acagcgtgca gcgtaaacac atgcttgaac
    24841 tggcgattgc cgacaagcca ttatttactc ttgatgaacg cgagctaaag cgcaatgccc
    24901 cctcttacac tgcgcaaaca ctgaaagagt ggcggcagga acaaggaccg gacgtgccgc
    24961 tggcgtttat tattggtcag gattcattgc tgacctttcc gacctggtac gaatacgaaa
    25021 cgatactcga caatgcacat ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg
    25081 cgcaaccgca ataccagcaa tggctggaag atcatttgac acataacccg gaagatcttc
    25141 accttcagcc tgccggtaaa atttatctgg ctgaaacgcc gtggtttaac atctcggcga
    25201 ccatcatccg cgaacgtttg caaaacggtg aatcgtgtga ggatttattg ccggaaccgg
    25261 tattgactta cattaaccaa caaggcttgt atcgctgata cctgtcgtta cggagggcat
    25321 gatgcgactg tggttaattc gtcatggcga aacacaagcg aatgtcgatg gtctgtacag
    25381 cggtcatgcg cccacacccc tgaccgcacg cggtattgag caagcgcaaa atctgcatac
    25441 actgctagat gatgtttcct ttgacttggt tttatgcagt gaactggaac gggcacagca
    25501 taccgcgcga ctggttctca gtgaccgcca gctccccgtt catatcatcc ctgaactcaa
    25561 cgaaatgttt tttggcgact gggagatgcg acatcatcgc gacctcatgc aagaagatgc
    25621 cgaaaactat agcgcgtggt gcaatgactg gcagcatgct atccccacga acggtgaagg
    25681 atttcaggca ttttcgcaac gtgtggaacg ctttatcgca aggcttagtg aatatcagca
    25741 ctatcagaat attttaatcg tcagccatca gggcgtactg agtctgttaa ttgctcgttt
    25801 aattggcatg cctgccgagt ctatgtggca ttttcgcgtt gaccaggggt gttggagcgc
    25861 cattgatatc aaccaaaaat tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt
    25921 cgagaacgca tgacttttct gtttttttac gggtaagccg caacgaccat tgacagcccc
    25981 gggcaggctg atattctccg cagccagact ttttccgcca gacacgactt tgtagaaatt
    26041 gttttacaaa aatggcgatg caatctgcgg cgcggggtgg gatgatagcc cactttcgaa
    26101 agccgattcg gcgacaattg tcccgaaatc gcctctggtt caggtatact gacagaccat
    26161 ttttatctat ttgattcacc cagggggaaa acttgcaggg taaagcactc caggattttg
    26221 ttatcgacaa aattgatgac ctcaaaggtc aggacatcat cgccttagac gttcaaggca
    26281 aatccagcat caccgactgc atgatcatct gtacgggtac gtccagccgt catgttatgt
    26341 ccattgctga ccacgttgtg caggagtctc gcgcagcggg cctgttaccg ctcggcgtag
    26401 aaggtgaaaa cagcgccgac tggattgtcg tggatttggg cgatgtgatt gtccatgtca
    26461 tgcaggaaga gagccgtcgc ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc
    26521 tgcaacttgt cgccgtggga acgaaaatgc cggactgggt acaaaccggt tttaccgagt
    26581 acctgcgtcg ttttccgaaa gatatgccct tcgagctgat tgaaattccg gccggcaaac
    26641 gcggcaagaa tgcggacatc aagcgcatac tcgacaaaga gggtgagcag atgttggcgg
    26701 ccgcaggcaa aaaccgcatt gtcaccctcg atattccagg caagccctgg gatacgccgc
    26761 agttagccgc tgagctggaa cgctggaagc tggatggtcg cgacgtaagt ctactgattg
    26821 gcgggcctga agggttgtcg cctgcctgta aagcggcggc tgagcagagc tggtcgctgt
    26881 cggcgcttac cctcccccat ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg
    26941 cgtggagcat caccaccaac catccttatc accgtgagtg ataagggagc tttgagtaga
    27001 aaacgcagcg gatgaaacta cagaactctt ttcgcgacta tacggcagag tccgcgctgt
    27061 ttgtgcgccg ggcgctggtc gcctttttgg ggattttgct gctgaccggc gtgcttatcg
    27121 ccaacctgta taatctgcaa attgttcgct ttaccgacta ccagacccgc tctaatgaaa
    27181 accgcattaa gctggtgcct atcgcgccca gccgcggcat tatctacgac cgtaacggta
    27241 tccctctggc cctcaaccgc actatctacc agatagaaat gatgccggag aaagtcgata
    27301 acgtgcagca aacgctggac gctttgcgca gcgtggtaga tctgaccgat gacgatattg
    27361 ctgcattccg aaaagagcgc gcacgttcac accgtttcac ctctattccg gtgaaaacta
    27421 acctaaccga agtacaagta gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg
    27481 aagttaaagg ctataaacgt cgttactatc cttacggttc ggcgttgacc cacgtcatcg
    27541 gctatgtgtc gaaaatcaac gataaagacg tcgaacgcct gaataatgac ggcaaactgg
    27601 ccaactatgc ggcaacgcat gatattggta agctgggcat tgagcgttac tatgaagacg
    27661 tgctgcacgg tcagaccggt tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc
    27721 gccagttaaa agaagtacca ccgcaagccg gacacgatat ttacctgacg ctggatctca
    27781 aactccagca atatattgaa acgctgctgg cgggtagccg cgcagctgtg gttgttaccg
    27841 aaccgcgtac aggtggggtg ctggcgctgg tttccacgcc tagttatgac ccaaacctgt
    27901 ttgttgacgg tatctccagc aaagattatt ccgccttgct gaacgacccg aatacaccgc
    27961 tggtgaaccg cgccacacag ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg
    28021 cggtttcggc attaagcgcc ggggtgatca cgcgcaatac gacgctgttt gacccaggct
    28081 ggtggcaact accagggtcg gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc
    28141 gtctgaatgt cacaagatcg ctggaagaat ctgcggatac cttcttctat caggtggcct
    28201 acgatatggg gatcgatcgc ctctccgaat ggatgggtaa attcggttat ggtcattaca
    28261 ccggtatcga cctggcggaa gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga
    28321 aacgctttaa aaaaccgtgg tatcagggtg acaccattcc ggttggtatc ggccagggtt
    28381 actggacagc gaccccaatc cagatgagta aggcactgat gatcctgatt aatgacggta
    28441 tcgtgaaggt tccgcatttg ctgatgagca ccgccgaaga cggcaaacag gtgccatggg
    28501 tacaaccgca tgaaccgccc gtcggcgata ttcattccgg ttactgggag ctggcgaaag
    28561 acggtatgta cggtgttgct aaccgcccta atggtacggc gaataaatac tttgccagcg
    28621 caccgtacaa aattgcggcg aaatctggta ccgctcaggt cttcggtctg aaagcgaacg
    28681 aaacctataa tgcgcacaaa attgccgagc gtttacgtga ccacaaactg atgaccgcct
    28741 ttgcgccata caacaatccg caagtggctg tcgccatgat tctggagaac ggcggcgcgg
    28801 gtccggcggt tggtacgctg atgcgccaga tcctcgacca cattatgctg ggtgataaca
    28861 acaccgatct gcctgcggaa aatccagcgg ttgccgcagc ggaggaccat taatcatgac
    28921 ggataatccg aataaaaaaa cattctggga taaagtccat ctcgatccca caatgctgct
    28981 gattttactg gcattgctgg tttacagcgc cctggttatc tggagcgcca gcggtcagga
    29041 tattggcatg atggagcgta aaatcggcca aatcgcgatg ggtctggtca tcatggtggt
    29101 gatggcgcaa attcctccac gcgtttatga aggctgggcc ccctatctct atatcatctg
    29161 tattattttg ctggtggcgg tagatgcttt cggagccatc tctaaaggcg ctcaacgctg
    29221 gctggacctc ggtattgttc gttttcagcc gtcggaaatt gccaaaatag ccgtaccact
    29281 gatggttgcg cgctttatca accgcgacgt ttgcccgcca tcgttgaaga atactggcat
    29341 cgcgctggtg ctgatattta tgcccacgct gctggtggct gcacagcctg acctgggaac
    29401 atcaatcctc gttgcgcttt ccggtctgtt tgtactgttc ctctctggcc ttagctggcg
    29461 tctgattggc gtcgcagtag tgctggtagc ggcgttcatt ccgattctgt ggttcttcct
    29521 gatgcatgat taccagcgcc agcgcgtaat gatgctcctg gacccggaat cagacccact
    29581 cggcgcgggc tatcacatta ttcagtctaa aattgctatt ggctccggcg gattacgcgg
    29641 caaaggctgg ctgcacggca ctcagtcaca gcttgaattt ctccccgaac gccatactga
    29701 ctttatcttc gcggtactgg cggaagagct gggattagtg ggcattctga ttctgctcgc
    29761 tctctacatt ctgctgatca tgcgcgggct gtggatagcc gccagagcgc aaaccacctt
    29821 tggtcgcgtc atggctggtg gcttaatgct gatattattc gtttatgtct tcgtaaatat
    29881 tggtatggta agcggtattc tgccggttgt aggggttccg ctcccactgg tcagttatgg
    29941 aggatcggcg ctaattgtgc tgatggctgg gttcgggatt gtaatgtcaa tccacaccca
    30001 caggaaaatg ttgtcgaaaa gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga
    30061 tctgcatcgc ggcaggaatg ctcgcggcat gtacaagcga tgatggtcag caacagacgg
    30121 taagtgtacc gcagcctgcg gtatgtaacg gccccatagt tgaaattagc ggggcggacc
    30181 cgcgtttcga accactgaac gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct
    30241 acaaaatcgt gcaggatccg tctcgattta gccaggcggg actggcggca atctatgatg
    30301 ccgaaccagg cagtaacctg acggcctctg gcgaagcttt cgatccgaca aagctgacgg
    30361 cggcccatcc aacgcttccg atccccagct acgccagaat cactaacctg gctaacgggc
    30421 gaatgatcgt ggtgcgcatt aatgatcgcg gcccttacgg caacgaccgc gttatttcgc
    30481 tttctcgcgc ggcagctgac cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc
    30541 cgattattgt cgcccaggat ggttcgcttt ctggtcctgg tatggcttgt accacagtcg
    30601 ccaaacagac ttacgccctg cctgcacctc ccgatttaag cggtggcgcg ggaacaagtt
    30661 cagtgtctgg cccgcagggt gacattcttc cggtcagtaa ttcgacgcta aaaagcgaag
    30721 atccgaccgg cgcgccggta accagtagcg gtttcctcgg cgctccaacg accttagcgc
    30781 ctggtgtact ggaaggcagc gaaccgacgc ctgcgccaca gcccgttgtt acagcttcgt
    30841 cgacaacgcc tgcaacctcg cctgcaatgg tgacaccgca agccgcctcg caaagcgcca
    30901 gcggcaactt tatggtgcaa gtcggggccg taagcgatca ggctcgtgcg caacagtacc
    30961 aacagcaact gggacagaag ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct
    31021 ggcggatcca gcttggccca ttcgccagca aagccgaagc cagtaccttg cagcaacgtt
    31081 tgcaaaccga agcccaatta cagtcattta ttactaccgc gcagtagcgt aaagcaggca
    31141 tctgatgtgt caatttctgt aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg
    31201 ccggtagtgc atttgctata gtagggcact tttttaattc catcacggat gtcgtagttc
    31261 agaccatgaa taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc
    31321 tttgcacagc ctttatctct gctgcacatg ccgatgacct gaatatcaaa actatgatcc
    31381 cgggtgtacc gcagatcgat gcggagtcct acatcctgat tgactataac tccggcaaag
    31441 tgctcgccga acagaacgca gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga
    31501 ccagttacgt tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca
    31561 ctatcggcaa cgacgcatgg gccaccggta acccggtgtt taaaggttct tcgctgatgt
    31621 tcctcaaacc gggcatgcag gttccggttt ctcagctgat ccgcggtatt aacctgcaat
    31681 cgggtaacga tgcttgtgtc gccatggccg attttgccgc tggtagccag gacgcttttg
    31741 ttggcttgat gaacagctac gttaacgcac tgggcctgaa aaatacccac ttccagacgg
    31801 tacatggtct ggatgctgat ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc
    31861 aggcattgat ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt
    31921 ttaacggtat tcgccagctg aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg
    31981 acggcatcaa aaccggacac actgacaaag caggttacaa tcttgttgct tctgcgactg
    32041 aaggccagat gcgcttgatt tctgcggtga tgggtggacg tacttttaaa ggccgtgaag
    32101 ccgaaagtaa aaaactgcta acctggggct tccgtttctt cgaaaccgtt aacccactga
    32161 aagtaggtaa agagttcgcc tctgaaccgg tttggtttag tgattctgat cgcgcttcgt
    32221 taggggttga taaagacgtg tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag
    32281 ccagctatgt gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg
    32341 gaactatcaa cttccagctt gatggcaaaa cgatcgagca acgcccgctg gttgtgttgc
    32401 aagaaatccc ggaaggtaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc
    32461 atcactggtt tggttaaaaa ttgaacactt gaaagtgtaa tttccgtccc catatactaa
    32521 gcatcagtaa aaaaactccc gccttctggc gggagttgct atttaattac gttacgccgg
    32581 agctgacatg aaaaccaaac ttaacgaact gcttgaattc cctactcctt ttacttacaa
    32641 agttatgggg caggcgttac ctgagctggt tgatcaggtg gttgaagtgg tacagcgcca
    32701 tgcgccaggt gactacaccc caacggtaaa accaagcagc aaaggcaact accactcggt
    32761 atctatcact atcaacgcca ctcatatcga gcaggttgaa acactgtatg aagaactggg
    32821 caaaatcgat attgtccgca tggttctgta actcgcttct ccgttacccg gctcctggtc
    32881 gggtaactcc cctctcctcg ctgtgatata cttttccccc acttttactc attctccacg
    32941 gagatgccgt tttgtatcag gataaaattc ttgtccgcca gctcggtctt cagccttacg
    33001 agccaatctc ccgggctatg catgaattca ccgatacccg cgataatagt acccttgatg
    33061 aaatctggct ggtcgagcac tatccggtat tcacccaagg tcaggcagga aaagcggagc
    33121 acattttaat gccgggtgat attccggtga tccagagcga tcgcggtggg caggtgactt
    33181 atcacgggcc gggacaacag gtgatgtatg tgttgcttaa cctgaaacgc cgtaaactcg
    33241 gtgtgcgtga actggtgacc ttgcttgagc aaacagtggt gaataccctg gctgaactgg
    33301 gtatagaagc gcatcctcgg gctgacgcgc ctggtgtcta tgtcggggaa aagaaaattt
    33361 gctcactggg tttacgaatt cgacgcggtt gttcattcca cggtctggca ttaaacgtca
    33421 atatggatct ttcaccattt ttacgtatta atccttgtgg gtatgccgga atggaaatgg
    33481 ctaaaatatc acaatggaaa cccgaagcga cgactaataa tattgctcca cgtttactgg
    33541 aaaatatttt agcgctacta aacaatccgg acttcgaata tattaccgct taattccata
    33601 catcaatggc ccaatttaca ctgggtcatt actcataata cctgctaatg ctttacatct
    33661 ccttcgcgtt tgtatatttt ctttatcact tgcagaaatg cggtgccacg ccttgctccg
    33721 taactcaccc cattgattaa cctattgaaa tagttgatta tggtgagcaa aagcgttccg
    33781 ttccaattag acataagatt attgtaattt tttaaatatt atttttccat gaataaaatt
    33841 ggagtaagtg cgtggatagt aataatcaaa ttgaaccctg tttaagcaga aagtcgtcag
    33901 aaggtaaacc acaaatattt acaaccttac gaaatattga tcttaacctt ctgactattt
    33961 ttgaagctgt atatgtacat aaagggatcg ttaatgcagc gaaagtgctt aatctgaccc
    34021 cctcggcaat cagtcagtct attcagaaac tgcgcgttat attccctgac ccattgttta
    34081 ttcgcaaagg ccagggtgtc actcctaccg catttgcgat gcatctacat gagtatatca
    34141 gtcagggcct tgagtccatt cttggcgcgc tggatatcga aggaagctat gataagcaac
    34201 gaacgataac tattgcgacc actccctcgg tcggagccct ggtccttcct gtcatctatc
    34261 gggcgattaa aactcactat ccgcagcttt tactgcgcaa cccgcccatc agcgacgcgg
    34321 aaaaccaact cagtcagttt caaaccgatc tcatcatcga taacatgttt tgcaccaatc
    34381 gtacggtgca acatcatgtt ctgttcaccg acaatatggt gttaatttgc cgcaagggaa
    34441 atccactact ctctttagaa gatgacagag agactatcga caacgctgcg catgtacttc
    34501 tgttaccgga agggcaaaat ttcagcggtc tgcggcagag agttcaagag atgtttccgg
    34561 accggcaaat caatttcacc agctacaaca ttttgacaat cgctgcactg gttgccaaca
    34621 gtgacatgtt agcgattatt cccagccgtt tttataacct gtttagtcgt tgctggccgc
    34681 tggaaaaact gccttttccg tccttaaatg aggagcaaat agatttctct atccactaca
    34741 acaaattcat cctgcgtgat cccatcctgc acggagtaat agatgttatc cgtaatgcat
    34801 tttgaaaaag tattgttaca gccagatcac agcgcagaac gataaaatgg cacaatccaa
    34861 caacaatttt acattttcgc gaccgctttg gctgctttca ggtccgtttc aatgatatac
    34921 tgccagtcgt taattcaaaa atagttgata attacaacaa tctattgaat tgaaacgctt
    34981 tccttcgtaa ttcgcaactg gaacacgcac gctatgagta aacccattgt gatggaacgc
    35041 ggtgttaaat accgcgatgc cgataagatg gcccttatcc cggttaaaaa cgtggcaaca
    35101 gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa tcaagcttcc agcggactct
    35161 acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag
    35221 gaagcctcct gccctaacct ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc
    35281 ctcggcgcta tttgtacccg ccgttgtccg ttctgtgatg ttgcccacgg tcgcccggta
    35341 gctcctgatg ccaatgaacc agtgaaactg gcgcagacca ttgccgatat ggcgctgcgt
    35401 tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg atggcggtgc ccaacacttt
    35461 gcggattgta ttactgccat tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg
    35521 ccggatttcc gcggtcgaat ggatcgtgct ctggatattc tgactgcaac gcccccagat
    35581 gtgttcaacc ataacctgga aaacgtaccg cgtatttacc ggcaggtacg gcctggcgca
    35641 gattacaact ggtcgctgaa gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg
    35701 accaagtctg gtctgatggt gggtctgggt gaaaccaatg aagaaattat tgaggtaatg
    35761 cgcgacctgc gccgtcatgg tgtgacgatg ttaacgctgg ggcaatattt gcagccaagc
    35821 cgccatcacc tgccggttca acgttacgtt agcccggatg agttcgacga aatgaaagcc
    35881 gaagcgctgg cgatgggctt tacccatgct gcatgcggtc cgtttgtccg ctcttcttac
    35941 cacgccgatt tgcaggcgaa aggaatggaa gttaagtaat cctgtaataa ttacttacat
    36001 ctgtctgata aaaaacccgc tttttgaagc gggttttttg tatcgaacaa gatattgagg
    36061 gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga agatcaacgt
    36121 ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac cctttaatgg
    36181 ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc agaacgacca
    36241 gcgccgcaac taccagcagt ttggtaatac taatctcacc catagatacc ttcttgacat
    36301 ataaccggca acagaacgcg ccttaaaaac cagagaatat taacggtcat tcgacgcgcg
    36361 cacacaatag caatctgtaa caaggtgaat caagcgccga gtaaaaaaac atcataataa
    36421 ttgcggcggc gcaaagcgac ggttgtttaa gatgggcagt tgcgcgcgca cctgacgcac
    36481 acgttcgggc gtcacttccg ccataatgag tgcaggcatt tctgacgctg ccgcaatggt
    36541 gacgccaaag ggatctataa tccggctttg cccgatattt ttgttcccgc actcccccgc
    36601 cgccaccata taacaggtgg tatccagcgc acgagcggca agcaacgttg accagtgatg
    36661 ctctttgagc ggcccacgaa cccaggcggc aggaagtacc aggatttcag ctccctgtaa
    36721 agcctgtgcc agcgccagct ctggaaagcg taagtcataa caggtcatca gaccgacctt
    36781 catcccttcc acctccagta acggagctat ttcattacca gcatcaacat ggcgtgattc
    36841 ctgaatggca aatgcatcat agagatgcag tttggcataa cgggcgacga tgtttcctgc
    36901 ctgaagtgcc accagcatat tccatgcgcg ccccggcgtt gaaggaagat gaatcgtcag
    36961 aattgtcgtc atcatgttac gtttactttc tcgccgtaaa cgtccgagga attcgccttc
    37021 cagcagctgt gctgatttaa ccgatagatc tgcatcatga tcatcgcgcg ccagcaatgc
    37081 ttccggcagg acaaacagcg atacgtcgtt ttccgccgcc tgcgccatca acgaggcaca
    37141 aatctcagcg ttcttttccc acacagatgt aacagcaaac tgtcctgcag caaccagcat
    37201 tgccacctcc tgtcagagtc gtaaaattcg ccaacgacga ttattcggcg ttacacttat
    37261 cactcataca aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta
    37321 ttggcggtgg tacgggaagc gtagcgagat ggctgttaag tatgcgattt aacccgctgc
    37381 atcaggcgat tccgttgggg acgctggcag caaacctgat tggggcattc atcataggaa
    37441 tgggattcgc ctggttcagc aggatgacga acattgatcc agtgtggaaa gtattaatca
    37501 ccaccggatt ttgtggcggt ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt
    37561 tgttacaaga gggccgcttt ggctgggcat tactgaacgt tttcgtcaac cttctggggt
    37621 cttttgccat gaccgcactg gcattctggc tgttttcggc ctcaaccgta cactaaagac
    37681 caaaaaaaac ccgctgatta agcgggtttt gaattcttgc tgacgtatct tacagagcga
    37741 ttacgtttgc agcagaaggg cctttggcac cgttagtgat ttcgaactct acgcgctgac
    37801 cttcagcaag agttttaaaa ccattagtct ggattgcaga gaagtgtacg aacacgtctt
    37861 tgctgccgtc ttccggagta atgaaaccga atcctttgga ctcattaaac cacttaacgt
    37921 tacctttaat cttagacatc aaaattacct ttacatgaaa aatagacaca aatgctgtgt
    37981 cggttatcag tacaccaatt gtggtacgct ttgtccagtg caactttact aaaaagtgat
    38041 aaaagtcgcg ttatttttga tgttgcttat tttagttatt gattttaaag aagttactaa
    38101 aacttcattt gtctcaaaac tgaaagcgca tccaggcaaa gtacacattg ccattgttgt
    38161 aggtacccgg aatgtaggtc atctgaaaag tcactgggcc ataacccacg gaggccaatg
    38221 gcagtagaac cgggagaggg atgtaattcc agttatcgcg tgccgttacg ccagcggtga
    38281 atcccagacc taaatgaaaa ttttcatccg ccagcggtcg ccaggtactt tcccatccgt
    38341 atccggcaat cggttcccat ttgttccacg agtccttaaa tgccatggca tacaggccat
    38401 gccagtttcc tttttcatcc caacgcgaca ggccaaaacc gccaccccac ggtcgctcgt
    38461 tatagcgatc ggttttttct ttgtcgtaag cgaaacgtgc atgccaggtg atggcaggaa
    38521 tatataaatc ataatgttca ggctgttgcc aggtttgtgc aatattttct ctaaacgttg
    38581 tcatcctctc atctgcgtta gcaaaaactt taccaacgct gattaactga ataaaaacaa
    38641 aggaaaagat agcgacatat ttactcacgt tcatttgtga ccataaaaca tttatcaaaa
    38701 atctactact agcatagcaa agctacaatt aacataacct taatagaccc aacataaaga
    38761 ataatctgaa tagctgctct atttatttcc gcttgttcaa gttaaatatt tccgcgcatc
    38821 gattaaagat gatgcgtcgt tttctgcgat gggaatagtc aaaaaagaaa aaccaagtct
    38881 tttttgatga caaaatgcaa tcaaggaaaa attattttat ttttttaacg agatacaaca
    38941 atcatcttaa cgaagtatat aatatatatc gtcaacccat gctcatatta gattattttt
    39001 tgagcgcaca gccgcagcta acaccattgc aattaacaaa tttgcatcaa tccaccatca
    39061 atttacacac attattatgt gataattgcc aaccgctaaa tatgcgtttt gttatctatg
    39121 tataaaaaca gcaacttcaa tgtcttaatg gcagtttttc ttgattttaa tcagcattca
    39181 tcgccaattt attgggcata ttttttcctt aagctttagg aagtttttat tatttacttt
    39241 gggcctggag acaggaaaaa ttatgctgac attcattgaa ctccttattg gggttgtggt
    39301 tattgtgggt gtggctcgct acatcattaa agggtattcc gccactggtg tgttatttgt
    39361 cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt taccgtccag
    39421 ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa tattactaat
    39481 gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggctttg ccgcttacat
    39541 gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat tgcagtatat
    39601 taactcccct tacctgctga tgattgccgc ctattttgtt gcttgtctga tgtcactggc
    39661 cgtctcttcc gcaaccggtc tgggggtttt gctgatggca accctgtttc cggtgatggt
    39721 aaacgttggt atcagtcgtg gcgcagcagc tgccatttgt gcctccccgg cggcgattat
    39781 tctcgcaccg acttcagggg atgtggtgct ggcggcgcag gcttccgaaa tgtcgctgat
    39841 tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg gcatggcgat
    39901 cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc atgaaatgtt
    39961 agatgtcagt gaaatcacca ccactgcccc cgcgttttat gccattttgc cgttcacgcc
    40021 gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca tcatcactat
    40081 tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct ttaataccca
    40141 gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt ttgctaacgt
    40201 ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca tcggctttat
    40261 tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc tgatgctggt
    40321 attggtgatt ctgaccatgc tggcggcagt cacgaccggt tcaggcaatg cgccgtttta
    40381 tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc cggcgtattt
    40441 gactattccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg tttctggcgt
    40501 agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa aacgcacctc
    40561 ggtgccggtg cttgttgggc tagtgattgt tatcgttgct acagagctga tggtgccagg
    40621 aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt tcctccggct
    40681 ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc gtgaacaatt
    40741 tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc agtggtgcgg
    40801 ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg ctctttaaac
    40861 agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggcagttc gtctttgggt
    40921 tcaccgcgag cataggcatt aaacatctca tcaatttgct tctggctggc gctatcaata
    40981 ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag tgtattgatt
    41041 cacttgaagt acgaaaaaac cgggagaaca ttggattatt cgggatctga tgggattaga
    41101 tttggtgggg cttgcaagcc tgtagtacaa attttaggcg ttaaccaatg aaacgcgaaa
    41161 gatagtaaaa aattgctttt gtttcattga aaaaacgaaa aacaaaaaca ctataaatca
    41221 tttcaatcat agcttcaaaa aacgttcaaa accgataaca accaggctgt ca
//
LOCUS       DAJTYM010000026        40105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTYM010000026 DAJTYM010000000
VERSION     DAJTYM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..525)
                     /locus_tag="OO575_001405"
     CDS             complement(<1..525)
                     /locus_tag="OO575_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6664425.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGL"
     gene            771..1397
                     /gene="gstB"
                     /locus_tag="OO575_001406"
     CDS             771..1397
                     /gene="gstB"
                     /locus_tag="OO575_001406"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR6664426.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1394..2508)
                     /gene="yliI"
                     /locus_tag="OO575_001407"
                     /pseudo
     CDS             complement(1394..2508)
                     /gene="yliI"
                     /locus_tag="OO575_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2619..3002)
                     /gene="bssR"
                     /locus_tag="OO575_001408"
     CDS             complement(2619..3002)
                     /gene="bssR"
                     /locus_tag="OO575_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6664427.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3215..4540
                     /gene="rimO"
                     /locus_tag="OO575_001409"
     CDS             3215..4540
                     /gene="rimO"
                     /locus_tag="OO575_001409"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6664428.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4587..5915)
                     /locus_tag="OO575_001410"
     CDS             complement(4587..5915)
                     /locus_tag="OO575_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6664429.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(5923..8265)
                     /locus_tag="OO575_001411"
                     /pseudo
     CDS             complement(5923..8265)
                     /locus_tag="OO575_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8443..9354)
                     /gene="gsiD"
                     /locus_tag="OO575_001412"
     CDS             complement(8443..9354)
                     /gene="gsiD"
                     /locus_tag="OO575_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6664430.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9357..10277)
                     /gene="gsiC"
                     /locus_tag="OO575_001413"
     CDS             complement(9357..10277)
                     /gene="gsiC"
                     /locus_tag="OO575_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6664431.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10295..11833)
                     /gene="gsiB"
                     /locus_tag="OO575_001414"
     CDS             complement(10295..11833)
                     /gene="gsiB"
                     /locus_tag="OO575_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6664432.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(11853..13724)
                     /gene="gsiA"
                     /locus_tag="OO575_001415"
     CDS             complement(11853..13724)
                     /gene="gsiA"
                     /locus_tag="OO575_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6664433.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13711..14676)
                     /gene="iaaA"
                     /locus_tag="OO575_001416"
     CDS             complement(13711..14676)
                     /gene="iaaA"
                     /locus_tag="OO575_001416"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6664434.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            14880..16115
                     /gene="moeA"
                     /locus_tag="OO575_001417"
     CDS             14880..16115
                     /gene="moeA"
                     /locus_tag="OO575_001417"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6664435.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16115..16864
                     /gene="moeB"
                     /locus_tag="OO575_001418"
     CDS             16115..16864
                     /gene="moeB"
                     /locus_tag="OO575_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6664436.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17043..17705)
                     /gene="fsa"
                     /locus_tag="OO575_001419"
     CDS             complement(17043..17705)
                     /gene="fsa"
                     /locus_tag="OO575_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6664437.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            17836..18735
                     /locus_tag="OO575_001420"
     CDS             17836..18735
                     /locus_tag="OO575_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6664438.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            18741..21173
                     /locus_tag="OO575_001421"
     CDS             18741..21173
                     /locus_tag="OO575_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6664439.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21319..22134
                     /gene="ybiV"
                     /locus_tag="OO575_001422"
     CDS             21319..22134
                     /gene="ybiV"
                     /locus_tag="OO575_001422"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6664440.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22286..23551
                     /locus_tag="OO575_001423"
     CDS             22286..23551
                     /locus_tag="OO575_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6664441.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(23792..25384)
                     /locus_tag="OO575_001424"
     CDS             complement(23792..25384)
                     /locus_tag="OO575_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6664442.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25603..26523
                     /gene="ldtB"
                     /locus_tag="OO575_001425"
     CDS             25603..26523
                     /gene="ldtB"
                     /locus_tag="OO575_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6664443.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26581..27699)
                     /locus_tag="OO575_001426"
     CDS             complement(26581..27699)
                     /locus_tag="OO575_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6664444.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27696..28163)
                     /gene="mntR"
                     /locus_tag="OO575_001427"
     CDS             complement(27696..28163)
                     /gene="mntR"
                     /locus_tag="OO575_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6664445.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28349..28477
                     /gene="mntS"
                     /locus_tag="OO575_001428"
     CDS             28349..28477
                     /gene="mntS"
                     /locus_tag="OO575_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6664446.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            28749..30332
                     /gene="opgE"
                     /locus_tag="OO575_001429"
     CDS             28749..30332
                     /gene="opgE"
                     /locus_tag="OO575_001429"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6664447.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30380..30895)
                     /gene="ompX"
                     /locus_tag="OO575_001430"
     CDS             complement(30380..30895)
                     /gene="ompX"
                     /locus_tag="OO575_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6664448.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(30948..31013)
                     /locus_tag="OO575_001431"
     CDS             complement(30948..31013)
                     /locus_tag="OO575_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664449.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31248..32135
                     /gene="rhtA"
                     /locus_tag="OO575_001432"
     CDS             31248..32135
                     /gene="rhtA"
                     /locus_tag="OO575_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6664450.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32434..32937
                     /gene="dps"
                     /locus_tag="OO575_001433"
                     /gene_synonym="pexB"
     CDS             32434..32937
                     /gene="dps"
                     /locus_tag="OO575_001433"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6664451.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33341..34087
                     /gene="glnH"
                     /locus_tag="OO575_001434"
     CDS             33341..34087
                     /gene="glnH"
                     /locus_tag="OO575_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6664452.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34226..34885
                     /gene="glnP"
                     /locus_tag="OO575_001435"
     CDS             34226..34885
                     /gene="glnP"
                     /locus_tag="OO575_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6664453.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            34882..35604
                     /gene="glnQ"
                     /locus_tag="OO575_001436"
     CDS             34882..35604
                     /gene="glnQ"
                     /locus_tag="OO575_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6664454.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            35865..38089
                     /gene="ybiO"
                     /locus_tag="OO575_001437"
                     /pseudo
     CDS             35865..38089
                     /gene="ybiO"
                     /locus_tag="OO575_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38086..39012)
                     /gene="rlmF"
                     /locus_tag="OO575_001438"
     CDS             complement(38086..39012)
                     /gene="rlmF"
                     /locus_tag="OO575_001438"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6664455.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39288..39548
                     /locus_tag="OO575_001439"
     CDS             39288..39548
                     /locus_tag="OO575_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6664456.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            39813..>40105
                     /locus_tag="OO575_001440"
     CDS             39813..>40105
                     /locus_tag="OO575_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664457.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNV"
BASE COUNT         9990 a        10589 c         9768 g         9758 t
ORIGIN      
        1 caggccgtgc accgtctgga aggtagtgtt ggtcagcccc agttttttgg cataaccatt
       61 catcagacca ataaaggact cctggctccc ggcaacgtaa tctgccagcg caatacaggc
      121 gtcattaccg gactggataa tcacaccttt gttcaagtct gccaccgaaa cctgatcgcc
      181 cggtttgagg aacattaccg atgaaccacg cagtgccgga tttcccgtcg cccaggcatc
      241 tttaccgacc gtcaccatat cggtgagttt aatcttatcg gccttaagcg cctgcccaac
      301 cacatagctg gtcatgattt tagtcaggct cgcaggatcc agtttctcat ccgcattgcc
      361 ttctgccagc actttaccgc tggcgtaatc cattaaaatc catgcacgcg catccacgct
      421 cggcgcttca acggtttgtt cggccgcgaa tgccgttggg gcaaaaagga ataaaaatgc
      481 agaacccgct gcaagaccac gaaggagaga ggagtattgc gtcataaatg ccacccgagt
      541 atccattcaa aaaaatacgt tatatcgccg tatgataagt tcggtgatta ataacgcaca
      601 cacaaactta aagaaaccgt aagttggtaa agtttttaaa gtttatgcaa tatcctgccg
      661 cttcgctgct tctggtgtca tttttttgcc agctgttgct taaaagttaa ctttacttca
      721 ccatcggagt cctgttcagt ctgtggctgc ctgtcggaaa aggggtgagt atgattacgc
      781 tgtggggtcg gaataattca actaacgtaa aaaaagtatt gctgacgctc gaagaactgg
      841 aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg gataaatcac gatgctgatt
      901 ttctggcgat gaaccctaac gggctggtgc cgttgttgcg tgacgacgaa agtgatctca
      961 ttctttggga atcaaacgcc attgtccgct atctggcggc acagtacggg caaaaacgcc
     1021 tgtggatcga ctcaccggca cgtcgtgcgg aagcggaaaa atggatggac tgggcaaacc
     1081 agacgctcag taatgctcat cgcgggatcc tgatgggatt agtcagaaca ccaccggaag
     1141 agcgcgatca ggccgccatt gatgccagtt gcaaagagtg cgacgccctg tttgccctgc
     1201 tcgatgcgga actggcaaaa gtaaaatggt tctctggcga cgagtttggt gtgggcgata
     1261 tcgctatcgc accgttcatc tacaatttgt ttaacgttgg cctgacctgg acaccgcgtc
     1321 caaatctgca acgctggtat cagcaactca ctgaacgccc cgcagtgcgc aaagtggtga
     1381 tgattcccgt tagctaattg cgtgggctaa ctttaagtaa ttccccactg gactcgtcgg
     1441 tgagaacgta taaataaccg tcgtgtccag tgcgaacatc acgaattcgc tgccctctgt
     1501 ccgttaaaat acggccatct tctgtcactt tgtcgccgtt gacgctcatc acaatgacat
     1561 ctttatcttt cagctcccaa taaataattt ttgctgccac tgggggaatt tatcgctgtt
     1621 atagaaggcc atgccgctca cagcgggcga atctttccag taaaaaacag gttgctcggt
     1681 cccggcgacg atctcccctt tcgcttccgg tatcttaaag cctgaatagt tgattcccca
     1741 ggttgccagc ggccagccgt agtttttgcc tttttgcggg atattaattt catcaccacc
     1801 gcgcgggcca tgttcattca gccacagtgc attactccac ggattcatcg ccattccttg
     1861 cggattacga atgccataag accagatctc ggcgcgcgca ccggattcct ttataaaagg
     1921 attatcatcc gggatttcgc cctggtcggt cagacgcacc agtttgccct gtaatttatc
     1981 cagatcctgc gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa gataaccttt
     2041 accgtcgaat accagccgcc cgccaaaatg gttgccggta gacagttttg gcatctggcg
     2101 gaagacggtg cggaagtcgg tcacttttga gagatcatca cttaagcggc cataacccac
     2161 agcagttccg gctttgccat catcgccaac ttcggaataa cttaaccaga tgcggcgaga
     2221 ctgagcaaaa tcaggcgcta aaaccacgtc cagcaggccg ccctgcccgt gcgcccaaac
     2281 gtccggaact ccggaaagcg gcgcagataa tccttttcct gcttgccagt gacgcaactc
     2341 gccgccgcgc agagtgatta acataccgtg attatcgggt aaaaaggcca gtgcccaggg
     2401 atggtcgagt ttgtcttgca gtacttcgac atttaccgtt gcaggagccg cccagagagc
     2461 ggaagaaaga caaataaggg gcacaaggaa aaaggattgt cgatgcataa cgcctccgga
     2521 ttgactctgg cttaaagcgt agtcagtgga ggagataaca aattcatttt tacaaaaact
     2581 taaacatgaa gggggagacg ctttctcccc cttagttttc aggccttctc aagcatggcg
     2641 tgcttctgca ggctctggat actcagcgtt aagctcatca gacaattttc aagcttatcg
     2701 gcgttgacgg taataacagt cgggcaatca tggtgcccac tcatcaaaca tactgcggct
     2761 gtcgctaatg cttcttcagc atgatgaaga gcactccact cttcctgatc cagatgaaga
     2821 ttcaaccgca gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt atctcgtaga
     2881 tgattgcttt cgctgacgga catgtatcct tttgccttcc tcagttgcac gtatgcagcg
     2941 aggtcaacgc gtgcgtcgat caaccggttc agcagatcga ttcgctgtct gtcaacgaac
     3001 atacttcgat cctcctcttt ccagccttgt tgcaattaaa tagtatggct attttttgag
     3061 caaaatgctg tccgggatca attcttcacg ccgaattaac aaaatttaca tttccttcgc
     3121 ctccgccagc gaatgagatt gcgatacaaa ttttatccag aactctgtag aatccctgcc
     3181 ctgacctttc ctgtaactga atatttttta agctatgagc aaagtaactc cccagccgaa
     3241 aatcggcttt gtttcccttg gctgtccgaa aaaccttgtc gattcagagc gtattctcac
     3301 cgaactccgc actgaaggtt atgacgtggt accgagctat gacgatgcga acatggtgat
     3361 cgtcaacacc tgcggcttta ttgacagcgc ggtacaagaa tcactggaag ccattggtga
     3421 agcgttgaat gaaaacggca aggtaattgt gaccggttgt ctgggggcaa aagaagatca
     3481 gatccgcgaa gtccacccga aagtgctgga aatcaccggg cctcatagct atgagcaggt
     3541 tctggagcac gttcatcact acgtgccaaa accgaaacac aacccattcc tgagcctggt
     3601 gccagaacaa ggtgtgaaac tgacgccgcg tcattatgcc tatctgaaaa tttcggaagg
     3661 ctgtaatcac cgctgcacct tctgcattat tccgtctatg cgcggcgacc tgatgagccg
     3721 tccgattggc gaagtgttaa gtgaagcgaa acgtctggta gatgcgggcg ttaaagagat
     3781 tctggtgatc tcgcaggata cttccgccta tggcgttgat gttaaacatc gtactggctt
     3841 ccacaacggc gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt tatcgaaact
     3901 ggggatctgg acacgtctgc actacgttta cccttatccg catgtggacg acgtcatccc
     3961 actgatggca gaaggcaaaa tcctgccgta tctggacatt ccgttgcagc acgccagccc
     4021 gcgcattctc aaactgatga agcgtccggg ttctgtagat cgccaactgg cgcgcatcaa
     4081 acagtggcgc gaaatctgcc cggaactgac cctacgctca acctttattg tcggcttccc
     4141 tggcgagacg gaagaagatt tccagatgct actcgacttc ctgaaagaag cacgtctgga
     4201 tcgcgttggc tgctttaaat acagcccggt tgaaggtgca gacgccaatg ccctgcctga
     4261 ccaggttccg gaagaagtga aagaagaacg ctggaaccgt ttcatgcagc tgcagcagca
     4321 gatttccgcc gagcgcctgc aagagaaagt gggccgtgaa attctggtga ttatcgacga
     4381 agtggacgaa gaaggcgcga ttggtcgcag catggcagat gcaccggaaa tcgacggcgc
     4441 ggtttatctc aatggtgaaa ccaacgttaa gccgggtgat atcctgcgtg tgaaagtcga
     4501 gcacgccgat gagtacgatt tgtggggtag ccgggtttaa ttaattcagg ccgggcaaaa
     4561 tcatttgtcc ggcctgcgaa taatgattac tgccagctaa tctgtgccct gccgtttttc
     4621 ttactcttat acagccgctc atccgccagc attttcaagc gttcgacggt agtacattca
     4681 tttgaatgcg ctacaccgat actggtgctt aaattaatgc ttcccacctg gaaatgaacg
     4741 atttctttta gtatcgatag caactcttcc agcgtgccgc cagtataaat caccgcaaat
     4801 tcgtcacctc cgaagcggta gactttacct ttatctaccg ggatctgtga tttaaggatt
     4861 ttcaccactt tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc gttaatgttt
     4921 ttgaatttat cgacgtcaaa cagacacaaa taaccgcctt tttccggcag cgcttttaaa
     4981 tcttcatcaa aggattttct gcccattgct ttggtcaggt cgtccaggaa cgccgagtta
     5041 tacatactgt agagtttttt gacatcggca gccgaaaaga aaagcccgcc atccagtacg
     5101 cgttcaaaat ggttattaat gatggcttct ttctccacaa taccacgatt aatcactctg
     5161 cgtaccagat agaggcaact ggcacccaat atcactgcca ccaatgcggc aaagaaaata
     5221 atcaatgtac aaacccgcaa ataaacatct tttttatacg caaatacgta caatccggtc
     5281 ggcaaatagg cgaagtgctc catcgcttcc gaaacggtga agatggcgtt tttagcatta
     5341 tattcatcac cggtcaccgg tttaccgttc ttaaatagag agaacatcag gttctcgctg
     5401 tccatcacca gcgattttga cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga
     5461 ctacgattgt gagttaccgg atagaggtaa tagacgaagg ttttaccgtc aaaagagata
     5521 aaccctgtat ttttgacatg tcgatacttc acaacctgca taatatctgc acaataattt
     5581 ttctcgaact cctttacctg aatacattcg tcactggaaa tcagataagg accgacatcg
     5641 tacaaataag agggggaaac cgagtcggcg taagctgtgc gcaaaccaac caacagctgt
     5701 tcctccgtgg gatttagcgt gttgataaga tcaattgcca cgctacgccg atattcagca
     5761 atatttgcca ccgcaaaagc agtatcaata acggactgtt tcaggctata cacccgttcc
     5821 tttaccattt gagtgtagat cccgcaggca actgctgaaa tcatgataaa aatcagcaga
     5881 ctgactgtaa gtacgaactt attgattctg gacatacgta aactactctt ttactaattt
     5941 tccactttta tcccaagcgg agaatggcat gggtttctgg tacagatacc cctgagcctg
     6001 aacacatcct aatttaatta attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat
     6061 caccgggatc cggttatagc gtgccagatc gaaaataatg cgcacaattt ttcccgcagt
     6121 accgaaaata tcgccggtaa attgcgagtc cacctttatg ctgtctggat taatttcata
     6181 aaaacgtttc aggtttgaca aaccggtgcc aaagtcatca atcgatatcc gtaccccttg
     6241 tttacgcagt tgttcaatat tgagataaaa atgttcatta attcgctcga tcacggtttc
     6301 agtgatctca acacagaggc tgttcgcctt caggccattt tctttcatga tggtggtaaa
     6361 acgctcatga aagtgcggtt cgtttaactg ccagggcgaa acattaatat gcagtaagaa
     6421 atcatccttc agcgacgcac gttgactaaa atgtctgaat taacccattg ccgttttaat
     6481 aatctgataa cccagctcat tgataaaccc gatatcttca gcaatggtaa tgaacttcag
     6541 cggtgaaatg atcccccgct ccgcagaaac ccaacggcac aacgcctccg cgctcagaat
     6601 ggcgcgggtg ttaatatcaa cgatcggttg atagacgagg aatagttcgc caatttccat
     6661 tgcatggcga atattacgcg caatccagat attctcttct atttcgttga gtttcgcgtc
     6721 atcacagaaa ataaactcgg agaacatatg gtcacggata ctggaaatcg ccagtttcag
     6781 tttttctgcg taactccagg cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg
     6841 gatgcgcaga ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg ctattttacg
     6901 ggtaaatgtc tgtacatttt cccgacacag aagtaaatag agatcggcat tgaatgtcac
     6961 gatatcgcag cattgcgcat atttctcggc aattcgctga cacaatttcc tgacgatttt
     7021 attgcgttca tcaacaccaa acaccgcctc cacggcttcc agaccacaca gtttgatgat
     7081 ccccacagcc agatactgat tattgttgga ctcaataatc gccatatttt taataaaacc
     7141 gctttcctga tcgtaagaga tcagtgagag caatttattt gaccgttcaa tttcggcatc
     7201 tttcatttcc gcgatcgaat ttttgatgct gataatctct ttcgcatcgc cttcgcgaat
     7261 tttcagagcc tcgatctgat ccgttgagtc gggcaaaaac gccagcgtat taaccagctc
     7321 ggtcatttca gaaaattgcc gacggaacac acgagtggtc atgaaatata ccaccagcaa
     7381 cacgataata atgaacgcaa acggtagata aaaaagttta tctgatgcgt tcaataaatt
     7441 accgccagga taataaaaat agatttcgaa caatgcatta tttggcactg ctgatgtctt
     7501 tgcaatatac tccgtatcga cagaatcaat aatattataa ttaccttcgc tattataaat
     7561 aatcagtttt tcacgagcga gttgattctt ggcaatgatc tcatgacctt gtttcaaaaa
     7621 tagaacttca tcattagcgc tgatttgatc aagatactcg ttcagatacc gccagtttaa
     7681 atcgacatga attttatcta ccgctaacaa atcgtgatct ttgccgttga tacgtagcgt
     7741 aaaagagata cattttttac ccggacgatc aaagcaattg tatgccggat tccagctgtt
     7801 gttagagtca tctgaagcca ccgaccacgg tcgtttcgat gggttaaaat cggcaaaaat
     7861 aacccgccct gactcgtcga caagcggtcc atttacttgt tcctgatgat aaacaacgat
     7921 tttatcattc gccctccgcg aaaagagata atatttatta tcatcaagta ccagtcccac
     7981 cgagtctaaa aatggcatca tttccatttt cagaatcacg gcgcgttgaa tctcctctgt
     8041 attttttaat aaatacagtg agtcctttaa tgtcagggcc gaacgttcaa tatctttaaa
     8101 gaaatagcca acgtttttct gaataagttc aaagcggcta tcggcacgtt ttaccgcgcg
     8161 ttccgatacc agttgatagt taatcatgaa aaaaagacca ataaatgaca gtgctgccaa
     8221 taaaaataaa attatcagcc ttatcttttc gtataaactc agcatgttaa tacatttaac
     8281 aaaataacta tctgaacgct gtgccgctgc aaaaatagtc ccttgctgcg gctttttaac
     8341 caaattaccc gattctacaa aatcagcaga aagttacaac acttgcttag ggcaataatt
     8401 tcctgtgagg tgattaccct ttcaagcaat attcaaacgt aactatcctt taattttcgg
     8461 atccagcgca tcgcgtaaac catcgcccaa caaattgaac gccagtacgg tcagaaaaat
     8521 agccagggcc ggaaaaacag cgacatgcgg cgcgataacc atatccgctc gtgcctcatt
     8581 gagcattgct ccccactctg gtgtcggcgg ctgcgcaccg aggccgagaa atgacaggct
     8641 ggcggcagag ataatcgagg tgccaatgcg catggtgaaa aacaccacga tagaagagac
     8701 ggtcccaggc aggatatgac gcaataaaat ggtcatatcg ctggcaccaa tactgcgtgc
     8761 tgactcaata aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca ggcgggcaaa
     8821 cgcggggatg gaaaaaatgg cgactgcgat aatcacgtta gcaatgccgc ttcccaacac
     8881 cgcaacaaca gcgatcgcca gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg
     8941 catgatcagc cgatcccacc agccttcata atatccagcc aacaagccca gcaacgtccc
     9001 tatcgccaca ccgataaaca cggcaaacac gcccgccgcc agcgagattt gcgcaccaac
     9061 caggacacgg ctgaaaatgt cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga
     9121 aggtccgtta ttcagattgt cataatcaaa ataattttcg gcgtcatagg gagcgatcca
     9181 gcgtgcaaaa atggccacca caatcaataa aataacgaat aatgcggcgg tcatcgccat
     9241 atgctgacgg cgaaatcgtc gccagaattc atgccacggt gtacgtacct ggtcaggttt
     9301 gaccagtggc atggcgttta acaccgcctg acgtcgccag ttaaatagtc gcatccttac
     9361 ttgtacctga tagccgggtt aatggcggcg taaagcacat ccaccactaa gttgataaga
     9421 ataaattcca gcgagaaaag cagaatttcc gcctgaatca ccgggtaatc gcgcatttct
     9481 acagagtcaa cgagtaagcg tccaagtccg ggccagttga aaactttctc caccacgata
     9541 gaaccaccga gcaaaaagcc aaactgtaag cccatcatgg tcactaccgg gatcatcgcg
     9601 ttgcgtagcc cgtgtttgag gacaacccag gtttcgctca cccctttcgc cctcgcggta
     9661 cgcatataat cttcgcttaa aacgtcgaca aatgacgcac gggtaaagcg cgccatcacg
     9721 gcggccactg ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca actgtctgct
     9781 cccacagtag gcagccagcc cagttcaacg gagaatacct gaattaaaaa catccccagc
     9841 gcaaatgccg gaaacgagat ccccgacacc gcaatggtca tactcaatcg atccggccaa
     9901 cgattacgcc agacggcggc gataattccc gccgccatac caaatatcac cgcccagacc
     9961 atactggtta tggtcagcca tagcgttggc ataaagcggc tggcaatctc atcggcaacc
    10021 ggacgacgcg acaccatcga caggccaaaa tccccctgca cagcattgct gatatagtgc
    10081 cagaactggt gatacagcgg ctgatccaac cccagctgct gacgcaccag ttctataacc
    10141 tgcgcatcag cttcgggccc ggcaatcaat cgcgccggat cgccgggcag catgtggaca
    10201 aataaaaaca ccagcaccga gacgataaac agcgtcggaa tcaaccccag taatcgtttg
    10261 ataacgtaat aaagcattcc actccctgcg ttgcttattg caaatccgcg tcttcaaagc
    10321 tgaagccggt atctggcatg atccaaaaac cggtcaggtt tttactgtgt gccgacacca
    10381 gtttttctac caccagcggg atccacggcg attcttgcca gatgatatcc tgcgccgcct
    10441 tatataagcg ggtcttttcc gccggatcat tcgttttcag tgcctgagcc aggaagtcat
    10501 ccacctgttt attgctgtaa aacgcggtat taaacagcgt cggtggccag ttttgcgagg
    10561 caaacagcgg cgatagtgcc cagtcggctt cgccggttga agccgaccag ccagtgtaga
    10621 acatccgcac gccactctct ttttgccctt taccttcaac ttctgccgcc cgctgtccgg
    10681 catccatcgc ggtcacctgg gctttaatcc cgacctgcgc taactgctgc tgggtaaatt
    10741 gcagcacttt ctgcgcggtg ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt
    10801 tgggatatcc cgcctctttc agtaattcgc gcgctttcac tggatcgtaa ggccacggtt
    10861 tataactttg cgcgtaggcg atacttggcg gtaccacacc agtagctgga gttgcatagc
    10921 ccgcaaaggc aactttcacc agcgccggac ggttaatggc gtaattcagc gcctcacgga
    10981 ccttcgggtt atcgaacggc ttttgcgtca cgttcatact gatataacgc tgcataattg
    11041 acggactggc catcaactcg atatttttgt ttttctccag cagtgtggcc tgctcgtagg
    11101 gaatggggaa agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat
    11161 ccgccaccgg acgccaggtt atgctgtcca gtttgggcaa tcctggctgc cagtaacccg
    11221 cgaatttttt caccttcaca aaatcggtct gattccaggt atccagttca tacggtccgg
    11281 ttcccaccgg atgaaaacca atctccttgc catatttttc cagcgctgtc ggtgaaatca
    11341 tcgcggtcgc cggatgggca agaatattaa taaacgctga gaacggctgt ttgagggtaa
    11401 tctttaccgt tgtcggatcg atcgcctccg ttttggcaat attcttatac aggttatagc
    11461 gtttaagatg attcgccgga tcgctggccc ggtccagatt cgcttttacc gccactgcgt
    11521 tgaaatcggt gccatcctgg aatttaattc cttcccgcaa tttcacggtg taagtaatgc
    11581 cgtcatcgga aacggtataa ctctccgcca gcacgttttt cagtttcatc tctttatcca
    11641 gaccgaacag cccctggtaa aacgatttcg ctacggcctg agataacgtg tcattcgcgt
    11701 catacggatc gagcgtggtg aaattcgatc ctaccgccac caccacatct ttggcagcga
    11761 atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta
    11821 cagctcttgc cattttattc tccgcctgaa tgttatctac gcatgaatgc gtattctgat
    11881 tgtggttgtg cgacgtaatg ccccggcccg acgcattgca acgagacggc tgccacctct
    11941 tcgccacgca gatgaatatt gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt
    12001 tgtcgggacg gttcagcgac cggaactgcc gccagtaatt tacgcgtata aggatgctgc
    12061 gggttttcga agaccgcgca ccgtgaacca atttcaacaa tttgcccgag atacatcacc
    12121 gccacacgat gactaatccg ctctaccaca gccatatcgt gggagataaa cagatacgca
    12181 atgccgaaat cacgctggag atcgagcaac aagttgataa tctgcccgcg aatagaaaca
    12241 tccagcgccg aaacggcttc gtcggcaatg atcacttttg gattcaatgc caacgcgcga
    12301 gcaatgcaga tgcgctggcg ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt
    12361 tcaggtaaca ggcccacgcg ctccagcaac cacgcaacgc gtgcagccgc gtctttacct
    12421 ggcaataaac cgtgtacacg cagcggttcg ataatcgaat caccgatggt ctgacgtggg
    12481 tccagcgaag cgtaagggtc ctgaaaaata aactgaatat cccggcgcaa tgcctgaagt
    12541 ttgccaggtg acaaggtatc gattcgctga ccgttaaaga taatttcgcc gccctgcgat
    12601 tcgaccaggc gcagcaacgc ccgcccggta gtggatttac cgctgccaga ctcgcccacc
    12661 agcgatagcg tttcgccagg ccagagatca aaactgactt tctcaacggc atgcacttcc
    12721 cgcgttacgc gattcaacaa accgctgcgc aaagggaaac gggtgacaag attacgcacc
    12781 cgtaaaacag gttcgccatc caccaccgtt ttctgctcga tggggggttc ctgtttcgct
    12841 ggatgttcaa gcgatatcaa tgggaaacgt cggggataat ctaacccttt catcgcacca
    12901 agttgcggaa cagcagctaa cagcgcacgg gtgtaaggat gttgcggtgc atgaaaaatc
    12961 tgttcgacgg tacccgtttc caccgcctcg ccctgataca tcaccagtac ccgatcggca
    13021 atctctgcca ccacgcccat atcgtgagtg ataaagataa cgcccatcga catctctttt
    13081 tgcaatactt tgattaattg caggatctgc gcctgaatag tgacatccag cgcggtggtt
    13141 ggctcatcgg caatcagcac cgccgggcgg catgacagcg ccatcgcaat catcactcgc
    13201 tggcgcatcc cgccagagag ttgatgcgaa taacgtgaaa gaatggtttg tgcctccgga
    13261 atgcgtacct gatccagcat ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc
    13321 tgatgcagac gaattgattc ggcaatctgt tcacccacag taaatactgg gttcagcgat
    13381 gtcatcggct cctgaaatat catcgccata tccgcaccgc gcacatggcg catttgtgca
    13441 gcgctctgct cgctaagttc aatcacatca cgactgcgcc gccgcaacag cattttatcg
    13501 cactgtacta aaccgcccgc ctgttccaac agacgcatca atgccagcgc agtcactgac
    13561 ttaccggagc cggattcgcc tacaattgcc agcgtctcac cgcgttgcag actaaaagag
    13621 agattgcgga ccgcagctat tttctgctgg tcctgcataa aggcaatatt aagattttca
    13681 accgccagca cattaccggc atcaagttca tcactgtgtg gcaacggtgt cccctttttc
    13741 acggtagata ccggtggttg gcgtatcgcc tgcgtagccc caggcgcgat acattccttc
    13801 ggtgttaaac ggtagcgcga cattcccttc atggtcgata gcgattaagc caccgctacc
    13861 gccaagcgca gggagttttt ccattactac ccgctcgcag gcttccgcga gacttaatcc
    13921 gccgtaatcc attaacgcgg cgatgtcata tgccgccagc gcgcggatga agacttcgcc
    13981 tgtgccggta caagaaaccg ccacactggc gttattggcg tagcatccgg cacccactaa
    14041 gggactatcg ccaactcgtc cgggtaattt attggtcatt ccgcctgtgg acgtggctgc
    14101 cgccagattg ccgtctaaat ccaacgccac ggcccccacg gtgcccattt tctgtttttc
    14161 atccagtggc gcaccgctat ggtcgaggac tgttgcccct tcctcgcgcg ctgccagtag
    14221 ttgttcataa cgcaaaggcg tggagaaaat ctccggtgag acgcgctcca tgccatgagc
    14281 aaacgcaaaa ttttctgccc cttcgccaat catcatcaca tgcgggcttt gctccatcac
    14341 cagccgggcg gcaagaaccg gattacgcag atgactaacg cccgccaccg caccggcttt
    14401 cagggtgtta ccatccatca cacaggcgtc cagttcatgg gtttcatcac gcgtaaagac
    14461 agcgccaatt ccggcgttaa acagtggaca ctcttccagc agacgcaccg cttccgtcac
    14521 cacatccagc gcactttcgc cggcttccag cattttctgc ccggtttcaa caatggcaga
    14581 caacgcctcg atgtagcgta attcctgttg cagactcatc tgcgcgcggc taattgcacc
    14641 tgcgccacca tgaattgcaa tgactgcttt gcccatagta ttcgtccatt aagggtggct
    14701 cgccactttt gctataaata tcagaatttt tatgaattac tcattcaatt tttgaatata
    14761 gaaagatgga tatggcatgt aaaggcagga gccaacgcta cgcccgaggt ggcatacagt
    14821 ctatggcgtt tttctgacat aataggcaaa ttcgattttg cctccgcagg agtgttttca
    14881 tggaatttac caccggattg atgtcgctcg acaccgcgct taatgagatg ctttctcgcg
    14941 ttaccccact gaccacccag gaaacgctgc cactggttca gtgttttggt cgtattctgg
    15001 cgagcgatgt cgtttcgcca ctggatgtcc cggggtttga taactccgct atggacggct
    15061 acgcggtgcg tttagccgat attgcctccg ggcaaccgct gcccgttgcc ggtaaatcct
    15121 ttgccggtca gccataccat ggtgaatggc ctgcgggtac ctgcattcgt attatgaccg
    15181 gtgcgccggt gccggaaggc tgcgaagcgg tggtgatgca ggagcagact gaacaaacgg
    15241 acaatggcgt gcgttttact gctgaagtgc gtagcgggca aaatattcgc cgtcgcggtg
    15301 aagatatctc tgcaggtgcg gttgttttcc cggcgggaac tcgcctgact accgcagagc
    15361 tgccagtaat tgcttcactg gggattgccg aagttccggt gattcgtaaa gtgcgtgtag
    15421 cgcttttttc taccggtgat gaactccagt tgcccggtca gccgctgggc gacggccaaa
    15481 tctacgacac caaccgtctc gccgtacacc tgatgttaga acagctgggt tgcgaggtaa
    15541 tcaacttagg gattatccgc gacgatccac atgccctgcg cgccgcattt attgaagccg
    15601 acagccaggc ggatgtagtg atcagttccg gcggtgtttc agtgggtgaa gcggattaca
    15661 ccaaaatgat tcttgaagag ctgggggaga tcgccttctg gaagctggcg attaaaccag
    15721 gtaaaccgtt cgcgttcggt aaactcagca atagctggtt ctgcggcctg ccgggcaacc
    15781 cggtttcagc gacgctgacc ttctatcaac tggtacagcc tttgctggca aaactaagcg
    15841 gcaacaccgc cagcggcctg cccgcgcgcc agcgcgtacg cacagcgtcc cgcctgaaaa
    15901 aaacgccagg acgtcttgat ttccagcgcg gcgtgctgca acgcaacgcc gacggtgaac
    15961 ttgaagtgac gaccaccgga catcagggtt cacatatatt tagctccttt agtctcggca
    16021 actgctttat cgtgctggaa cgcgatcgcg gcaatgtgga cgtgcgcgaa tgggtggaag
    16081 tagaaccgtt taacgcgttg ttcggaggcc tgtaatggcg gaactcagcg atcaggagat
    16141 gctgcgctac aaccggcaaa tcattctgcg cggttttgat tttgacggtc aggaggcgct
    16201 aaaagattct cgtgtgctgg tagtcggcct cggtggactc ggctgcgcag cttcgcagta
    16261 tctggcaagc gccggtgtcg gcaacctgac gctgctcgac ttcgacacgg tttcgctctc
    16321 gaatctgcaa cgccagacac tgcacagtga tgccacggtc gggcaaccga aggtggaatc
    16381 cgcccgtgac gccctgacgc ggatcaaccc acatatcgcg attacgccag tcaatgcact
    16441 gctagatgac gcagaacttg cagcattgat tgctgaacac gatctggtgc tcgactgtac
    16501 ggataacgtt gcggtacgta atcaactgaa cgcaggctgt tttgccgcga aggtgccgct
    16561 ggtttccggc gcggcaattc gtatggaagg gcaaatcacg gtcttcactt atcaggacgg
    16621 cgaaccgtgc tatcgctgcc ttagccgttt gtttggcgag aacgcgttaa cctgcgtaga
    16681 agcaggcgtg atggcaccgt tgataggcgt aattggttcg ttgcaggcaa tggaagcgat
    16741 caaactgctg gcaggttatg gcaaaccggc cagcgggaaa atcgtcatgt acgatgcgat
    16801 gacctgtcag tttcgcgaaa tgaaactgat gcgcaatccg gggtgtgagg tgtgcgggca
    16861 gtaattgcca cctccaagcc tgataagacg cgacaagcgt cgcatcaggc atttgctcca
    16921 gacgccagat gcggcgtgaa cgccttatct ggcctacggt tcgggcacag atttccaagc
    16981 gcggcaggca tcggtgcact ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc
    17041 ggttaaatcg acgttctgcc aaacgctccc tgccagtcct gctcaaactt cgtcacagcg
    17101 gcttccaccg ccggatagct aatcatctgt tgtgccacat ccagtggcag agtaattgat
    17161 tcacatccgg ccagtaagca gtccagcgcc tgacgcgggg ttttgaaact cgctgccagc
    17221 actttcgcct gcggcgcatg cattttcaat aactggtgta agtcggtcac agtctgaatg
    17281 ccgctaccgc cctgagcatc aatacgatta acgtaaggcg caacatattc cgcacctgcc
    17341 agcgccgaca gcagcccttg tgccgcgcca tataccgcgg ttcccagcgt cggaatccct
    17401 tccgctttta acatcttaat cgctgccagc ccctcggctg tcaccggaac tttcaccacg
    17461 atatccgcaa taatagaacg cagcttaagc gcgtcattca ccatcccttc agcagtggta
    17521 gccattacct gggcaaacag acgcccctga ccgcccatcg cttcatgaag ttgcggaagc
    17581 acaacatcca gcggtttttt acccgcggcg ataatgcttg ggttagtggt cacacccgcc
    17641 agcggaaaaa tacgtgacag cgccttcacc gcaacaacgt ctgaagtatc cagatacagt
    17701 tccataacca tcctcaaaat gtactttttg tctgaatgta gactatcacg ggaaaactct
    17761 ctgaacagtt gacgcacatc aatctaactt tcattcgaaa gtattttaat gtttgaacga
    17821 aacagagagg caactatgat tttcaatatt cagcgctact cgacccatga tggccccggt
    17881 atccgcacgg tcgtatttct taaaggctgt tcgctgggct gccgctggtg tcagaacccg
    17941 gaaagccgcg cccgtacgca ggatctgctg tatgacgcac gactgtgtct ggaaggctgc
    18001 gagctgtgcg ctaaggccgc gccggaagtg attgagcgcg cgctgaatgg tttgcttatt
    18061 catcgggaaa agttaacccc ggagcatctg acggcgttaa ccgactgctg tccgacacag
    18121 gcgttaaccg tgtgtggtga agtgaaaagc gttgaggaga tcatggcgac cgttctgcgc
    18181 gataaaccgt tttacgatcg cagcggcggc ggcttaacgc tttcgggtgg tgaacccttc
    18241 atgcagccgg aaatggcgac ggcgctgctg caagcctgcc acgaggcagg cattcatact
    18301 gcggtagaaa cctgtctgca tgtgccgtgg aaatatatcg ccccttctct gccctatatc
    18361 gatctgtttc ttgccgattt aaaacacgtt gccgacgcgc cgtttaaaca gtggaccgac
    18421 ggtaacgccg ccagggtgct ggataacctg aaaaaactcg ccgcagcggg caaaaaaatc
    18481 attatccgcg tgccgctgat tcagggcttt aatgccgacg aaacctctgt aaaagccatt
    18541 accgattttg ccgccgacga gctgcacgtt ggcgaaattc attttctgcc ctaccacacg
    18601 ctgggcatca acaaatatca cttacttaat ctgccctatg acgccccgga aaaaccgctt
    18661 gatgcgccag agctgctcga ttttgcccag cagtatgcct gccagaaagg gttaaccgcg
    18721 accttacgag gataacaacc atgaccacac tgaaactgga cacgctcagc gaccgcatta
    18781 aagcgcacaa aaatgcgctg gtgcatattg tgaagccgcc agtctgtacc gagcgcgcgc
    18841 agcactatac cgagatgtat caacaacatc tcgataagcc gatcccggta cgtcgcgcgc
    18901 tggcactggc gcatcacctg gcgaatcgca ccatctggat caaacacgat gagttgatca
    18961 ttggcaacca ggcaagcgaa gttcgcgccg cgccgatctt cccggaatat actgtctcgt
    19021 ggatcgaaaa agagattgat gatctggcag atcgtcccgg tgctggcttt gcggtgagcg
    19081 aagagaacaa acgtgttctg catgaagtgt gcccgtggtg gcgcggtcag acggtacagg
    19141 atcgctgcta tggcatgttt accgatgagc aaaaaggtct gctggcgacc ggcatcatta
    19201 aagcggaagg caatatgacc tccggcgatg cgcacctggc ggtcaatttc ccgctgctgc
    19261 tggaaaaagg gcttgatggt ctgcgtgaga aagtagcgga acgtcgctcg cgcatcaatc
    19321 tgacggtgct ggaagatttg cacggcgaac agttcctgaa agcgattgat atcatgctgg
    19381 tggcagtcag tgaacacatt gaacgtttcg ctgccctggc gcgtgcaatg gccgcgaccg
    19441 aaacccgcga aagccgtcgc gatgaactgc tggcgatggc agaaaactgc gatcttatcg
    19501 cccaccagcc gccgcagact ttctggcagg cgctgcaact gtgttacttc atccagttga
    19561 ttttgcagat tgaatctaac ggtcactcgg tgtcgtttgg tcgtatggat cagtatctct
    19621 acccgtacta tcgccgcgac gttgaactga accagacgct ggatcgcgaa cacgccatcg
    19681 aattgctgca tagctgctgg ttgaaattgc tggaagtgaa caaaatccgc tccggctccc
    19741 actcaaaagc ctctgcggga agtccgctgt atcagaacgt caccattggc ggacaaaatc
    19801 tggttgatgg tcaaccaatg gacgcggtga atccactctc ttacgcgatc ctcgaatcct
    19861 gcggtcgcct gcgttccact cagcctaacc tcagcgtgcg ttaccatgca ggaatgagta
    19921 acgatttcct cgacgcctgc gtacatgtga tccgctgcgg cttcgggatg ccggcattca
    19981 acaacgacga aatcgtgatc ccggaattta ttaaactcgg tattgaaccg caggacgctt
    20041 atgactacgc agcgattggt tgtatagaaa ccgccgtcgg tggcaaatgg ggctatcgct
    20101 gtaccggcat gagctttatc aacttcgccc gcgtgatgct ggcggcgctg gaaggcgggc
    20161 gtgatgccac cagcggcaaa gtgttcctgc cacaagaaaa agcgttgtcg gcaggtaact
    20221 tcaacaactt cgatgaagtg atggacgcgt gggatacgca aatccgttac tacacgcgta
    20281 aatcaatcga aatcgaatat gtcgtcgaca ccatgctgga agagaacgtg cacgatattc
    20341 tctgctcggc gctggtggat gactgtattg agcgagcgaa aagtatcaag caaggcggcg
    20401 cgaaatatga ctgggtttct ggcctgcagg tcggtattgc caacctcggc aacagcctgg
    20461 cggcagtgaa gaaactggtg tttgaacagg gcgcgattgg tcagcaacag ctggctgccg
    20521 cactggcgga tgacttcgat ggcctgactc atgagcaact gcgtcagcgg ctgattaacg
    20581 gtgcgccgaa gtacggcaac gacgatgata ccgtcgatac gctgctggct cgcgcttatc
    20641 agacctatat cgacgaactg aaacagtacc ataacccacg ctacggtcgt ggtccggttg
    20701 gcggcaacta ttacgcgggt acgtcgtcta tctccgctaa cgtaccgttt ggcgcgcaaa
    20761 ctatggcaac gccggacggg cgtaaagccc acaccccgct ggcagaaggc gctagtccgg
    20821 cctctggtac tgaccatctc ggccctactg cggtcattgg ctcagtgggt aaactgccta
    20881 cggcagcgat tctcggcggc gtgttgctca accagaaact gaatccggca acgctggaga
    20941 acgaatctga caagcagaaa ctgatgatcc tgctgcgtac cttctttgaa gtgcataaag
    21001 gctggcatat tcagtacaac atcgtttccc gcgaaacgct gctggaggcg aaaaaacatc
    21061 cggatcagta ccgcgatctg gtagtgcgtg tcgcgggtta ttccgccttc ttcaccgcgc
    21121 tctctccaga cgctcaggac gatatcatcg cccgtactga acatatgctg taatctcttc
    21181 ggcccggctg cggcgtcgcc gggctaaatc attcacatca tcaatttcat ccttactttc
    21241 attcgaaata taatttgtgc tctgcgtcac attgttatta cactgttacg gtttcatttt
    21301 cgagccagga gcacacctat gagcgtaaaa gttatcgtca cagatatgga cggtactttt
    21361 cttaacgacg ccaaaacgta caaccaacca cgttttatgg cgcaatatca ggaactgaaa
    21421 aagcgcggca ttaagttcgt tgtcgccagc ggtaatcagt attaccagct tatttcgttc
    21481 tttcctgagc taaaggatga gatctctttt gtcgcggaaa acggcgcact ggtttacgaa
    21541 catggcaagc aactgttcca cggcgaactg acccgacatg aatcgcggat tgttattggc
    21601 gagttgctaa aagataagca actcaatttt gtcgcctgcg gtctgcaaag tgcatatgtc
    21661 agcgaaaatg cccccgaagc atttgtcgca ctgatggcaa aacactacca tcgcctgaaa
    21721 cctgtaaaag attatcagga gattgacgac gtactgttca agttttcgct caacctgccg
    21781 gatgaacaaa tcccgttagt gatcgacaaa ctacacatag cgctggatgg cattatgaaa
    21841 cccgtcacca gtggttttgg ctttatcgac ctgattattc ccggtctaca taaagcaaac
    21901 ggtatttcgc ggttactgaa acgctgggat ctgtcaccgc aaaatgtggt agcgattggc
    21961 gacagcggta acgatgcgga gatgctgaaa atggcgcgtt attcctttgc gatgggcaat
    22021 gctgcggaaa acattaaaca aatcgcccgt tacgctaccg atgataataa tcatgaaggc
    22081 gcgctgaatg tgattcaggc tgtgctggat aacacatccc cttttaacag ctgacctctc
    22141 taccggagcc atgcgctccg gttttccctt ctttttacat cctgtacatc acatcaaatc
    22201 attaaacttg cattaactta tttataactt gtattgtcac atgcaaatta gcatagtttc
    22261 gaatgaaagt taatgcaagg tggttatggc ttctactttt accagcgaca cattgcctgc
    22321 cgatcacaaa gcagctatcc gtcagatgaa gcacgcgctg cgggcgcagc ttggcgatgt
    22381 ccagcagatc tttaatcagc taagcgatga cattgccacg cgagtggctg aaatcaacgc
    22441 actcaaagca cagggcgatg ccgtctggcc ggtgctgtct tatgccgata tcaaagcagg
    22501 tcatgttact gcagagcagc gcgaacagat taaacgtcgc ggttgtgcgg tgataaaagg
    22561 ccatttcccc cgcgaacaag cgctaggctg ggatcagtcg atgctggact atctgaaccg
    22621 caaccgcttt gacgaggtct acaaaggccc cggcgataat ttcttcggga cgctcagcgc
    22681 ttcacgtccc gagatttacc ccatctactg gtcgcaggcg caaatgcagg cccgccagag
    22741 tgaagaaatg gcgaatgcgc agtcgtttct caatcgtctg tggacatttg aaagtgatgg
    22801 aaagcaatgg tttaacccgg atgtgagcgt catctaccct gaccgtatcc gccgccttcc
    22861 gcccggaacg acctccaaag gtcttggagc gcataccgac tccggggcgc tggaacgctg
    22921 gctgcttcca gcgtatcagc acgttttcgc caacgtcttt aatggcaatc tggcgaaata
    22981 cgatccctgg catgcggcac atcgtacgga agttgaagag tacacggtgg acaacaccac
    23041 caaatgttcc gtgtttcgga cattccaggg ctggactgcg ctctcggata tgctgcctgg
    23101 tcaggggttg ctgcacgtcg tgcccattcc tgaagccatg gcgtacgtac tgttacgtcc
    23161 gctgcttgat gatgtgccgg aggatgaact gtgcggcgta gcgcccggaa gagtgttgcc
    23221 ggtatcagag caatggcatc cactgttaat tgaggcgtta accagcattc caaaactcga
    23281 ggccggagac tccgtctggt ggcactgcga cgtcatccat tccgttgccc ccgttgaaaa
    23341 tcaacagggt tggggcaacg tgatgtacat tcctgcggca ccgatgtgcg agaaaaatct
    23401 tgcctacgcg cacaaggtga aggccgcact ggaaaaaggc gcatcgccgg gcgacttccc
    23461 gcgcgaggac tatgaaacaa actgggaagg acgctttacg cttgccgacc tcaacattca
    23521 cggtaagcga gcgttgggca tggatgtttg agagttaatg gaagtgaatt gatgtgggat
    23581 taagaaaatc gattcgacgt tactcagatt gctgacaagt gcgctttctt aatgcctgat
    23641 gcggcgttaa cgccttatcc ggcctacaaa gtcctgcaga ttcaatatat tacaggtact
    23701 gcgtaggcct gataagcgta gcgcatcagg ctattttgca tttgtcatca gccgtcgtca
    23761 ccccgacagt atcggggtga cggataatca cttactcgat ccctttacta cgcaggtaat
    23821 cttcgtaatt accgctaaag tcgatcacgc gttccggggt gatttccaga atgcgggtcg
    23881 ccagggagct tacgaactca cggtcgtgtg aaacaaagat cagcgtgccc tgatacagtt
    23941 ccagcgccat gttcagtgac tcgatggatt ccatatccag gtggttggtc ggttcgtcca
    24001 tgatcagaat gttcggcttc tgcatcatta acttaccaaa cagcatccgc cctttttccc
    24061 caccggaaag cactttagct ggctttttga tgtcgtcctg gctgaacagc aaacgaccga
    24121 gaatgctgcg taccgcctgc tcgtcatcgc cttcctgctt ccactgactc atccattcga
    24181 acacggtcag atcattttca aactcatatt cgtggtcctg ggcgtagtaa ccaatgcgcg
    24241 cgttttcaga ccattttacg gtgccgctgt ccggttgcag atcgcccacc agcgttttca
    24301 gcagcgttga tttaccgacg ccgttggtac ccagtaccgc aagtttttca ccgacctcca
    24361 gcagcaggtt gagattttta aacagtggac cgttatcaaa ccctttggtc agaccttcca
    24421 cttccagcgc gttacggaac agtttcttat cctgttcaaa acggataaac gggttctgac
    24481 ggctggaggc tttcacctct tccagtttga ttttatcaat ctggcgcgcg cgcgaagttg
    24541 cctggcgaga tttcgaggcg ttggcgctaa agcggctaac gaaagattgc aactcagcaa
    24601 tctgcgcttt cttcttggcg ttatcggcca gcagacgttc acgcgcttgg gtcgccgccg
    24661 tcatgtactc atcgtagtta cccggataaa cgcgcagctc gccgtaatcc agatccgcca
    24721 tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga gatgatgatc atggtgctgt
    24781 cacgctcgtt cagcacctgt tccagccagc gaatggtgtc gatgtcgagg ttgttggtcg
    24841 gttcgtcgag caggagaata tccggctcag caaacagcgc ctgcgccaga agcacacgca
    24901 gcttccagcc aggagcaact tcactcatcg ggccgtagtg ctgttccact ggaattccca
    24961 cgccaagcaa cagttcaccg gcgcgagctt ccgcagagta accgtccatt tcgccgtatt
    25021 tcacttccag atcggccact ttatagccgt cttcttcact catttccggc aaagcataga
    25081 tgcggtcgcg ctcctgcttc acttcccaca actctttatg ccccatgatc accgtatcca
    25141 gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag cttaccaatg cgctcgttgg
    25201 gatcgaggga aacgttaccc agcgtcggct caaggtcgcc gccgagaatc ttcataaagg
    25261 tggatttacc actaccgttc gcgccaatca ggccgtaacg gttgccgccg ccgaatttga
    25321 cggaaatgtt ttcaaacaac ggcttactgc cgaactgcat ggtgacgtta ctggaaacta
    25381 acacaggcgt atcctgaaaa gagatatgac aaaccgcgca ttatgccata agtctgaaag
    25441 aagatcctgc tcctcgctta aggtctacac tcaagccaaa ggcaaaaaat gtgatttcgt
    25501 acacatctga tttcactgtg agctggaatg aacttataat gcgcttccaa tactctaata
    25561 ttctcaaccc aatggcctgc caggcacaaa atctcgctta acatgaatat gaaattgaaa
    25621 acattattcg cagcggcctt cgctgttgtc ggcttttgca gtaccgcctc tgcggtaact
    25681 tatcctctgc caaccgacgg gagtcgcctg gttggtcaga atcaggtgat caccattcct
    25741 gaaggtaaca ctcagccgct ggagtatttt gccgcggagt accagatggg gctttccaat
    25801 atgatggaag cgaacccggg tgtggatacc ttcctgccga aaggcggtac tgtactgaac
    25861 attccgcagc agctgatcct gccggatacc gttcatgaag gcatcgtcat taacagtgca
    25921 gagatgcgtc tgtattacta tccgaaaggg accaacaccg ttatcgtgct gccgatcggc
    25981 attggtcagt taggcaaaga tacgcctatc aactggacca ccaaagttga gcataagaaa
    26041 gcaggcccga cctggacgcc gaccgccaaa atgcacgcag agtaccgcgc tgcgggcgaa
    26101 ccgctaccgg ctgtcgttcc ggcaggtccg gataacccga tgggtctgta tgcactctac
    26161 atcggtcgcc tgtatgctat ccatggcacc aacgccaact tcggtatcgg cctgcgtgta
    26221 agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat tcctgttcga gaaagtaccg
    26281 gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag cgaccaccga gccagacggc
    26341 agccgttata ttgaagtcca taacccgctg tctaccaccg aagcccagtt tgaaggtcag
    26401 gaaatcgtgc caattaccct gacgaagagc gtgcagacag tgaccggtca gccagatgtt
    26461 gaccaggttg ttcttgatga agcgatcaaa aatcgttccg ggatgccggt tcgcctgaat
    26521 taatcgtcaa aacgtaaaac aaaggcggac tcataatccg ccttttttat ttgccagacc
    26581 ttaccaggcc gggatcataa ctaacaaaac gtatcccacc agcgccgccc acaacagcat
    26641 cggtattgaa tagagatgga aacgccacca gatgcggcga tcgttcgcca tacgtagcgc
    26701 aatcaaattt gccagcgatc ctggtaataa accaaagccc cccacgttta ccgcccatgc
    26761 cagtaataaa gacggaggta cataattgag cagcaaaatg gtactcggca cgttactgat
    26821 cacctgcgat aaaccgactg ccgttaacca taacccgggt tcagataaat gactcacgtt
    26881 acccaacacg ccttgcaacg ctggaagctg ggtcagtaaa tggacgtcga taaacatcgc
    26941 cataaacacc agcagcaacg tccagtccac actcaacacc acgcgacgcg ccagcagcac
    27001 aaagcccgcc gccacaatca ccagtcccca cagctcttgt ttgaactcca gcgccgtcag
    27061 aaagacgata tacagcccca gacaattcca caccagccgc ggtttccact ccggtgtttg
    27121 cacccccgta tggtattgca gcgcctttcc agggaaacaa caccagcaca acagcaggag
    27181 tgtcagcatc attgcgccag ccatcggtgc catttgggca ataaatccgg caaacgaaag
    27241 accagaacgt ccccagataa gaatattttg cgggttgcca attggcgtca gtagcgaacc
    27301 agcgttgact gccagcgcct caaaaataat cagccgatta accgggatct cacacagtct
    27361 ttttagcgtg atagtcagcg gaacaacaat aaacagcgcg acatcgttgg tcagaaaggt
    27421 agaaagcagc gccgccgcca gcaccataaa catcgccagc cgacgctccg tagcaaagcg
    27481 gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt aactccacac ctttggtcag
    27541 cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg atagcagcag gccaggattt
    27601 cggtgcaaag ggcacgaaaa agcttaatcc gataccaaca agaattaata actgaaaaaa
    27661 acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg tttctgggta
    27721 aacaaacgaa aagcgtcgag cgtctcttca ctaacatggt gctccatgcc ttccgcgtcg
    27781 cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac tatctgatgt
    27841 cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac gccacgccag
    27901 gggatcattt caatcagccc catggttgcc agccgcttaa gcattttagc caccgtcggt
    27961 tgcgaaactc ccagacgagc agccatgtcc acctgacgag cttcccccac ttccctgatc
    28021 aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc ctctctgacc
    28081 tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt ttttgctatt
    28141 ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata catagcgttc
    28201 tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta acgttttagc
    28261 aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc cctcaaggat
    28321 cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta tgcgcgtgtt
    28381 tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg atatggtcaa
    28441 caacaaaccg cagcaagata aaccttcaga taaatagcgg cgtcgcggta cgccgcttca
    28501 ctcctgcttt tatgcaggca taacgcgttt tggtctgaaa aaaaccactt tttgtcggat
    28561 ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg acctgaacgg cggctcgctc
    28621 tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt tcccctcttc
    28681 acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt ttgttacgat
    28741 agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg gtatttagcc
    28801 cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta ggttacccct
    28861 tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg cgaacattgc
    28921 ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt tatttccctt
    28981 ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac tccaccaata
    29041 tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg gtcggcttat
    29101 ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa aaagcgcgct
    29161 ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttt gttgctcccg
    29221 tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat ccggtatttc
    29281 gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa gaacggatgg
    29341 caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg aagtatcaga
    29401 cctatgtggt agtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc tttggcggtc
    29461 actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct gactacattg
    29521 ctgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgta gtcgatggca
    29581 aaccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc cagacctggt
    29641 ggttttccaa ccagggccaa atcggcgaat acgatactgc tatcgccagc atcgccaaac
    29701 gtgcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa aacacgaaag
    29761 acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg caaccgcagc
    29821 tgattgttct gcatctgatg ggctcgcatc cgcaggcctg cgacaggaca caaggcaaat
    29881 acgaaacctt tgtgcaatcg aaagaaacgt cgtgctatct ctataccatg acgcaaacgg
    29941 acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc ttctctctgg
    30001 tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg caataccttg
    30061 cccatgatga taaatatcag caaaatttcc aggtgccttt tatggtcatt tccagcggcg
    30121 ataaagcgca tcgggtgatt aaagcccgcc gctcagccaa tgacttctta ggctttttct
    30181 cgcagtggac gggaattaaa gcgaaggaaa taaatatcaa atacccgttt atatctgaga
    30241 agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat tacaaccatc
    30301 tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa atccgccccg agaggcggat
    30361 ttttatatca ccaaagtgat tagaagcggt aaccaacacc ggcaatccag gtgcctacgt
    30421 caacgctacg aatacggctc tgctcgtaag agaagtccag agcaacgttt tccatcgggt
    30481 tgaactgcag accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt ttgtaggtcg
    30541 ggtattcagt ggtctggaat ttaccataac ccacacccac tacaccgtag atgcttgccc
    30601 agtcgttaat gcggtaagcc ggaccagcag tgatgccgta gtactggttt ttgttgtagt
    30661 caccagagct tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc acacccagcg
    30721 ggctgttgtc ttcttcatag cggtatttca ggttgaaacc gcccattttg ttcatttggc
    30781 cctgagcgtc gctctgtgcg taaccgccag ttacggtaga agtcgcagct acggaagtac
    30841 ctgcggtgaa agccagaact gcggccagtg ctgaaagaca tgcaattttt ttcataacca
    30901 cctcaaatgt gattcaaata agtcctaagt tttaaatata tcaaaaatta atgggaaact
    30961 cttcgcgatt tgtgatgtct aacgggccat ttcatgtaac agaacgtttc catacaccgc
    31021 tatccatcta aatttaaatc actttttcag agaactgcgt aagtattacg catgttttcc
    31081 ctgtcattca tccagattat tcctaatcac cagactaatg attccatcaa tcctggcgca
    31141 ttttagtcaa aacgggggaa aattttttca acaaatgctc aaccagcatt gggtatatcc
    31201 agtacactcc acgctttact taagtctaga tatttgtggg agaaaggatg cctggttcat
    31261 tacgtaaaat gccggtctgg ttaccaatag tcatattgct cgttgccatg gcgtctattc
    31321 agggtggagc ctcgttagct aagtcacttt ttcctctggt gagcgcaccg ggtgtcactg
    31381 cgctgcgtct ggcattagga acgctgatcc tcatcgcgtt ctttaagcca tggcgactgc
    31441 gctttgccaa agagcaacgg ttaccgctgt tgttttacgg cgtttcgctg ggtgggatga
    31501 attatctttt ttatctttct attcagacag taccgctggg tattgcggtg gcgctggagt
    31561 tcaccggacc actggcggtg gcgctgttct cttctcgtcg cccggtagat ttcgtctggg
    31621 ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct ggggcaagac gtttcccatg
    31681 tcgatttaac cggctgtgcg ctggcactgg gggccggggc ttgttgggct atttacattt
    31741 taagtgggca acgcgcagga gcggaacatg gccctgcgac ggtagcaatt ggttcgttga
    31801 ttgcagcgtt aattttcgtg ccaattggag cgcttcaggc tggtgaagca ctctggcact
    31861 ggtcggttat tccattgggt ctggctgtcg ttattctctc gaccgctctg ccttattcgc
    31921 tggaaatgat tgccctcacc cgtttgccaa cacggacatt tggtacgctg atgagcatgg
    31981 aaccggcgct ggctgccgtt tccgggatga ttttcctcgg agaaacactg acacccatac
    32041 agctgctggc gctcggcgct atcatcgccg cttcaatggg gtctacgctg acagtacgca
    32101 aagagagcaa aataaaagaa ttagacatta attaaattta catttctgta tggttatgca
    32161 taaccatgca gaatttctcg ctacttttcc tctacaccgt atttatatat cgaattatgc
    32221 aaaagcatat ttattccgaa aattcctggc gagcagataa ataagaattg ttcttatcaa
    32281 tatatctaac tcattgaatc tttattagtt ttgtttttca tgcttgttac cactattagt
    32341 gtgataggaa cagccagaat agcggaacac atagccggtg ctatacttag tctcgttaat
    32401 tactgggaca taacatcaag aggatatgaa attatgagta ccgctaaatt agttaaatca
    32461 aaagcgacca atctgcttta tacccgcaac gatgtctccg acagcgagaa aaaagcaaca
    32521 gtagagttgc tgaatcgcca ggttatccag tttattgatc tttctttgat taccaaacaa
    32581 gcgcactgga acatgcgcgg cgctaacttc attgccgtac atgaaatgct ggatggcttc
    32641 cgcaccgcac tgatcgatca tctggatacc atggcagaac gtgccgtgca gctgggcggt
    32701 gtagctctgg ggaccactca agttatcaac agcaaaaccc cgctgaaaag ttacccgctg
    32761 gacatccaca acgttcagga tcacctgaaa gaactggctg accgttacgc aatcgtcgct
    32821 aatgacgtac gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga tatcctgacc
    32881 gccgcgtctc gcgacctgga taaattcctg tggtttatcg agtctaacat cgaataaatc
    32941 catcgctgat ggtgcagaac tttagtaccc gataaaagcg gcttcctgac aggaggccgt
    33001 tttgttttgc agcccacctc aacgcactta tttagtgcat ccatctgcta tccccagctg
    33061 attaagtaaa ttttttgtat ccacatcatc acacaatcgt tacataaaga ttgttttttc
    33121 atcaggtttt acgctaaata atcactgtgt tgagtgcaca attttagcgc accagattgg
    33181 tgccccagaa tggtgcatct tcagggtatt gccctataaa tcgtgcatca cgtttttgcc
    33241 gcatctcgaa aaatcaatga gttgcaaaat tggcacgatt ttttcatata tgtgaatgtc
    33301 acgcagggga tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg tattaaaagt
    33361 ttcactggct gcactgaccc tggcttttgc ggtttcttct catgccgcgg ataaaaaatt
    33421 agttgtcgcg acggataccg ccttcgttcc gtttgaattt aaacagggcg ataaatatgt
    33481 gggctttgac gttgatctgt gggctgccat cgctaaagag ctgaagctgg attacgaact
    33541 gaagccgatg gatttcagtg ggatcattcc ggcactgcaa accaaaaacg tcgatctggc
    33601 gctggcgggc attaccatca ccgacgagcg taaaaaagcg atcgatttct ctgacggcta
    33661 ctacaaaagc ggcctgttag tgatggtgaa agctaacaat aacgatgtga aaagcgtgaa
    33721 agatctcgac gggaaagtgg ttgctgtgaa gagcggtact ggctccgttg attacgcgaa
    33781 agcaaacatc aaaactaaag atctgcgtca gttcccgaac atcgataacg cctatatgga
    33841 actgggcacc aaccgcgcag acgccgttct gcacgatacg ccaaacattc tgtacttcat
    33901 caaaaccgcc ggtaacggtc agttcaaagc ggtaggtgac tctctggaag cgcagcaata
    33961 cggtattgcg ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg gcgcgttgaa
    34021 aaccctgcgc gagaacggaa cttacaacga aatctacaaa aaatggttca gtactgaacc
    34081 gaaataataa cgctacacct gtaaagcgca ctggcagttc cctctccccc ctggggagag
    34141 gattagggtg aggggcgcaa acccgctccg gggccattaa ttaccctgaa tttgattatt
    34201 tacaccacgg taacaggaac aacatatgca gtttgactgg agtgccatct ggcctgccat
    34261 tccgcttctg attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg gtctggctgg
    34321 cggtctggta atcggattgc tggcaggttt tgcacgcacc ttcggtggtt ggatagccaa
    34381 ccacgtcgcg ctggtcttta ttgaagtgat ccgcggcaca cctatcgtcg tccaggtgat
    34441 gtttatctat ttcgccctgc cgatggcgtt taacgactta cgcatcgacc catttactgc
    34501 ggcggtggtc accatcatga tcaactccgg cgcgtacatt gcggaaatca cgcgtggtgc
    34561 ggtgctgtct atccacaaag gttttcgtga agcaggactg gcgctcggtc tttcacgttg
    34621 ggaaaccatt cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc cgccgctggg
    34681 taaccagtgg atcatcagca ttaaagacac ctcgctgttt attgttatcg gcgtggcgga
    34741 actgacccgt caggggcaag aaattattgc cggtaacttc cgcgcccttg agatctggag
    34801 cgccgtggcg gtgttctatc tgattattac cctggtgctg agctttattc tgcgtcgtct
    34861 ggaaagaagg atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca ctttggccca
    34921 acccaggtgc tgcacaatat cgatttgaac attgcccagg gcgaagtcgt ggtgattatc
    34981 gggccgtccg gttccggtaa atcgaccctg ctgcgctgca tcaacaaact ggaagaaatc
    35041 acctccggcg atctgattgt cgatggcctg aaggtcaacg atccgaaagt tgacgagcgc
    35101 ctgattcgcc aggaagcagg tatggtgttc cagcagtttt acctcttccc gcatctgacg
    35161 gcgctgggaa acgtcatgtt tggtccgcta cgcgtgcgtg gcgcgaacaa agaagaggcg
    35221 gaaaaactgg cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc acatcactac
    35281 ccttccgaac tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc gctggcggtg
    35341 aagccgaaaa tgatgctgtt tgatgaaccg acttccgctc ttgacccgga actgcgccat
    35401 gaagtgctga aggttatgca ggatctggct gaagaaggga tgacgatggt gatcgtgacc
    35461 cacgaaatcg gttttgccga gaaagtagct tcgcggctga tctttatcga caaaggccgg
    35521 attgcggaag atggcaatcc gcaggtgttg atcaagaacc cgccgagcca gcgcttgcag
    35581 gaatttttgc agcacgtctc ttaataagac acattgcctg atgcgctacg cttatcaggc
    35641 ctacaggata tctggcaact tattaaaatt gcatgaactt gtaggacgga taaggcgttc
    35701 acgccgcatc cggcaaaaaa gcccgcacgt tgtcagcaac ctgcttaata tcccttcctc
    35761 cctttcaccc gaaagggagg cacaccagat tcctctcatt taaaatcgcc cctcccccag
    35821 catctatact tatctttttg ctctgttttc tcactggagg agtcatgcgg tggatcctgt
    35881 tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt atccataccc ggcgttacaa
    35941 ccacaacgac aacggactca acgactgaac cggccccgga accggatatc gaacaaaaaa
    36001 agcggcctat ggcgcactgg cggatgtgct ggataacgac acctcgcgta aagagttgat
    36061 cgaccagttg cgtactgtcg ccgcgacgcc ccctgcggaa ccggtcccga agatcgtgcc
    36121 gccgacgctg gttgaagagc aaaccgtgct gcaaaaggtc accgaagtca gccgccatta
    36181 tggtgaagcc ctttccgccc gcttcgggca actttatcgc aatatcaccg gctccccgca
    36241 taagccattt aatccacaaa ccttcagcaa tgcgctgagc catttttcaa tgttagcggt
    36301 attagtgttt ggtttttact ggctgattcg cctgtgcgca ctgccgctat atcgcaaaat
    36361 gggtcagtgg gcgaggcaaa aaaatcgtga gcgcagcaac tggttgcagc ttccggcgat
    36421 gattattggg gcgtttatta tcgacctgct gttactggcc ctgacattat ttgtcggcca
    36481 ggtattaagc gacaacctga atgcaggcag tcgcaccatc gctttccaac aaagtttgtt
    36541 tctcaacgcc tttgccctga ttgaattttt caaagccgta ctacgcctga ttttttgccc
    36601 aaacgtggcg gagctgcgcc cgttcacgat tcaggacgag agcgcccgtt actggggtcg
    36661 tcgcctgagc tggttaagca gcctgattgg ctatggcctg attgtggccg tgccgattat
    36721 ctctaatcag gtgaatgtac agataggtgc gctggcgaac gtcatcatta tgctgtgcat
    36781 gaccgtctgg gcgttgtacc tgatcttccg aaataaaaaa gagattaccc agcatatgct
    36841 caactttgcg gagcattcgc tggctttttt cagcctgttt atccgcgcct ttgcgctggt
    36901 gtggcactgg ctggcaagcg cctattttat catgctgttt ttcttttcgt tgttcgatcc
    36961 gggcaacagt ctgaaattta tgatgggtgc aacggtgcgc agcctggcga ttattggtat
    37021 cgcagcgttt gtttccggta tgttttcccg ctggctggcg aaaaccatca ccctctcgcc
    37081 acatactcag cgtaactatc cggagctaca aaaacggctg aatggctggc tttcggcggc
    37141 gctgaaaacg gcgcgtattc tgaccgtctg cgtggcagta atgttgctgt tgagcgcatg
    37201 gggattgttc gatttctgga actggctgca aaacggcgcg gggcagaaaa ccgtagatat
    37261 cctgatccgc atcgcactca ttcttttctt ctcggcggtt ggctggacgg tgctcgccag
    37321 tttgatcgaa aaccggctgg cttcggatat tcatggccgc ccgctaccca gcgcccgtac
    37381 gcgtaccctg ctgacgctgt tccgtaacgc gctggcggtg attatcagta ccatcaccat
    37441 catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca ttgctggcag gtgccggggc
    37501 attaggtctg gctatctcgt ttggttcgca aacgctggta aaagatatta tcaccggggt
    37561 atttattcag tttgaaaacg gcatgaacac tggagatttg gtgactatcg ggccgttgac
    37621 cggcacagtg gaacggatgt cgattcgctc cgtgggcgtg cgccaggata ccggggcgta
    37681 tcacatcatt ccgtggtctt cgataaccac ctttgctaac ttcgtccgcg gtattggttc
    37741 ggtagtggca aactacgatg ttgatcgcca tgaagatgct gataaagcca atcaggcact
    37801 gaaagatgcg gtaacggaat tgatggaaaa cgaagaaatt cgcgggctga ttattggtga
    37861 accgaacttt gccgggattg tcggcttaag caataccgcg tttacactgc gtgtttcgtt
    37921 caccacgctg ccactcaaac agtggacggt acgctttgcc ctcgacagcc aggtgaaaaa
    37981 acatttcgac ctggcgggcg ttcgcgcgcc agtgcagact tatcaggtgc tgcctgctcc
    38041 gggcgcgacc ccggctgaac cgctgccgcc gggggagcca acgctttaac gttggcgatt
    38101 aacaaaacgg cggcgctgct cgtcgttcat aaaggtccag gcaataaagc gactctgctt
    38161 ttgcccctgg gccatctctt tttttaccac cttcaccgcg cccacgtcgg tcagagcacg
    38221 atacaacggc ggtaagtttt caccacgaga taccagcgag gtaaaccaca tcacctgctt
    38281 cgcgaagcct ttgctctctt caatcatctt tttgataaag ttgacttcac cgccttcaca
    38341 ccacaactcc tgttgctgac caccaaagtt cagtgcatcg tctttgttca gccccaggtt
    38401 acggcgttta cgctcactac ctgcccgcgc cgcagcggcg gaatcgtgga atggcgggtt
    38461 acacaaggtc gcgtcgtatt gctcgttttt gtggatgatg ccgttaaaaa tcgccccact
    38521 ctctttttgc cgacgcagac gaatggcgcg gttaagcccc ggattagcac tgataatcgc
    38581 ctgcgcactg cttaacgcct ggctgctggt ttcactaccg gtaaaacgcc agccatattc
    38641 atgtacgcca atcagcggat aaatacagtt cgcaccaacg ccgatatcca gaatgctggc
    38701 attcgctgga attgttccgc ttgcttcagc cagtaaatcg gcaaggtgat gaatataatc
    38761 cgcccggccc ggtactggtg ggcagagaaa accgtcgggg atatcccagt tcgctacggc
    38821 gtaaaaatgg gccagcaacg ccttattgag cgccttcacc gccagcggat tggcaaagtc
    38881 tacgctttgc tcccctgcgg gtgtaagcgt gaggaattgc ctgagttcag gattgacctg
    38941 acaaagcgtg gcgagatcgt agcggctgtg atgacggttg cgcggatgca accccggttt
    39001 ctgggcggac atagcattct cctttaagca gcggcgtaag atacccgcat aacgcgacag
    39061 ggtaaatatc tggctgcgtt tatcattccc caaaacctta acatggctat ggtttatggc
    39121 aattcatcat aaaaatcagc attatgcatg tcattgattt gtcttatttt gtaaaaatat
    39181 atcgttcaaa aaataagcat ttctctgtca attttttgcg tcatttgcat cgcgcaggcg
    39241 acctacactt atcaggcact acccgttgta tttatgagga tgtcattatg aaaaagtgcc
    39301 tcacactact gattgccacc gtcctgagcg gaatctctct cacggcttat gccgcgcaac
    39361 cgatgagtaa cctggacagc ggtcagctac ggcccgccgg taccgtttcg gcaactggcg
    39421 catcaaacct aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa caaggcgcga
    39481 aaggttatgt cattaattcc gcaggcggaa atgaccagat gttaggtact gcaaccatct
    39541 acaaataacc ccaccaacgt aactgcattc gcctctggat gcagttttcg cctgttttgt
    39601 aatcaaaaaa catcaaatat gaacgcaatg taaataaatg tatttctttt tcgcgcaatg
    39661 ggtgatagaa aatcgcatca agtgataatg cttatcaaaa ttattatcac ttgcacgaac
    39721 actatcacgg gattaacagt ggcatcgcat ccgcagagat gctttctcgt ggcagtgaaa
    39781 atttcaacat ataagaaaaa gtcacctgca aaatggaaaa caatcgcaat ttccctgcca
    39841 gacaatttca ttcgctcacg ttctttgccg gtctttgtat tggtattacg cctgtggcac
    39901 aggcactcgc cgccgaaggg caagctaacg cggatgacac gctggttgtc gaagcatcaa
    39961 cgccttcgct ttatgcgcca caacaatctg ccgatccgaa attctcgcgt ccggtagcgg
    40021 atactacccg cacgatgacg gtgatttctg aacaagtgat taaagatcag ggcgcaacca
    40081 accttaccga cgcgctcaaa aacgt
//
LOCUS       DAJTYM010000027        39981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTYM010000027 DAJTYM010000000
VERSION     DAJTYM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..146
                     /locus_tag="OO575_001441"
     CDS             <1..146
                     /locus_tag="OO575_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6664458.1"
                     /translation="MLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQ
                     ELN"
     gene            375..965
                     /gene="uidR"
                     /locus_tag="OO575_001442"
     CDS             375..965
                     /gene="uidR"
                     /locus_tag="OO575_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6664459.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1353..3056
                     /gene="uidA"
                     /locus_tag="OO575_001443"
     CDS             1353..3056
                     /gene="uidA"
                     /locus_tag="OO575_001443"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6664460.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3062..4087
                     /gene="uidB"
                     /locus_tag="OO575_001444"
                     /pseudo
     CDS             3062..4087
                     /gene="uidB"
                     /locus_tag="OO575_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4126..5391
                     /gene="uidC"
                     /locus_tag="OO575_001445"
     CDS             4126..5391
                     /gene="uidC"
                     /locus_tag="OO575_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6664461.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5617..7125)
                     /locus_tag="OO575_001446"
     CDS             complement(5617..7125)
                     /locus_tag="OO575_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6664462.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7226..8401)
                     /gene="manA"
                     /locus_tag="OO575_001447"
     CDS             complement(7226..8401)
                     /gene="manA"
                     /locus_tag="OO575_001447"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6664463.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8600..10246
                     /gene="fumB"
                     /locus_tag="OO575_001448"
     CDS             8600..10246
                     /gene="fumB"
                     /locus_tag="OO575_001448"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6664464.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10389..11792
                     /gene="fumC"
                     /locus_tag="OO575_001449"
     CDS             10389..11792
                     /gene="fumC"
                     /locus_tag="OO575_001449"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6664465.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11789..12718)
                     /gene="tus"
                     /locus_tag="OO575_001450"
     CDS             complement(11789..12718)
                     /gene="tus"
                     /locus_tag="OO575_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6664466.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12794..14095)
                     /gene="rstB"
                     /locus_tag="OO575_001451"
     CDS             complement(12794..14095)
                     /gene="rstB"
                     /locus_tag="OO575_001451"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6664467.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14274..15593)
                     /locus_tag="OO575_001452"
     CDS             complement(14274..15593)
                     /locus_tag="OO575_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR6664468.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15818..16561)
                     /gene="rstA"
                     /locus_tag="OO575_001453"
     CDS             complement(15818..16561)
                     /gene="rstA"
                     /locus_tag="OO575_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6664469.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16690..17025
                     /locus_tag="OO575_001454"
     CDS             16690..17025
                     /locus_tag="OO575_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6664470.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17022..17744)
                     /gene="folM"
                     /locus_tag="OO575_001455"
     CDS             complement(17022..17744)
                     /gene="folM"
                     /locus_tag="OO575_001455"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6664471.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17781..19163)
                     /locus_tag="OO575_001456"
     CDS             complement(17781..19163)
                     /locus_tag="OO575_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6664472.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19349..20293)
                     /locus_tag="OO575_001457"
     CDS             complement(19349..20293)
                     /locus_tag="OO575_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6664473.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20817..22349
                     /gene="pntA"
                     /locus_tag="OO575_001458"
     CDS             20817..22349
                     /gene="pntA"
                     /locus_tag="OO575_001458"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6664474.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22360..23748
                     /gene="pntB"
                     /locus_tag="OO575_001459"
     CDS             22360..23748
                     /gene="pntB"
                     /locus_tag="OO575_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6664475.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23773..24807)
                     /gene="tqsA"
                     /locus_tag="OO575_001460"
     CDS             complement(23773..24807)
                     /gene="tqsA"
                     /locus_tag="OO575_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6664476.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25218..25583
                     /gene="mdtJ"
                     /locus_tag="OO575_001461"
     CDS             25218..25583
                     /gene="mdtJ"
                     /locus_tag="OO575_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6664477.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25570..25899
                     /gene="mdtI"
                     /locus_tag="OO575_001462"
     CDS             25570..25899
                     /gene="mdtI"
                     /locus_tag="OO575_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6664478.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25938..26759)
                     /locus_tag="OO575_001463"
     CDS             complement(25938..26759)
                     /locus_tag="OO575_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6664479.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26859..26942)
                     /locus_tag="OO575_001464"
     CDS             complement(26859..26942)
                     /locus_tag="OO575_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664480.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27035..27370)
                     /locus_tag="OO575_001465"
     CDS             complement(27035..27370)
                     /locus_tag="OO575_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6664481.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27767..29020)
                     /locus_tag="OO575_001466"
                     /pseudo
     CDS             complement(27767..29020)
                     /locus_tag="OO575_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29127..30038
                     /locus_tag="OO575_001467"
     CDS             29127..30038
                     /locus_tag="OO575_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6664482.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30173..31393
                     /gene="mlc"
                     /locus_tag="OO575_001468"
                     /gene_synonym="dgsA"
     CDS             30173..31393
                     /gene="mlc"
                     /locus_tag="OO575_001468"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6664483.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31518..32213
                     /gene="bioD"
                     /locus_tag="OO575_001469"
     CDS             31518..32213
                     /gene="bioD"
                     /locus_tag="OO575_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6664484.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="OO575_001470"
     CDS             complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="OO575_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6664485.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="OO575_001471"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="OO575_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6664486.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /locus_tag="OO575_001472"
     CDS             complement(34274..35128)
                     /locus_tag="OO575_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6664487.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="dmsB"
                     /locus_tag="OO575_001473"
     CDS             complement(35130..35747)
                     /gene="dmsB"
                     /locus_tag="OO575_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6664488.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..37796)
                     /locus_tag="OO575_001474"
                     /pseudo
     CDS             complement(35758..37796)
                     /locus_tag="OO575_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37857..38966)
                     /locus_tag="OO575_001475"
     CDS             complement(37857..38966)
                     /locus_tag="OO575_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6664489.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38949..>39981
                     /locus_tag="OO575_001476"
     CDS             38949..>39981
                     /locus_tag="OO575_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6664490.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVES"
BASE COUNT        10158 a         9700 c        10241 g         9882 t
ORIGIN      
        1 aaatgttaca gcacacgccg atcagacgtc tgggccaacc gcaagatatt gctaacgcgg
       61 cgctgttcct ttgctcgcct gcagccagct gggtaagcgg acaaattctc accgtctccg
      121 gtggtggggt acaggagctc aattaataca ctaacgaacc ggtaaacggt cgtatgtgtt
      181 gtttaccggg atagcgtgtg caattcatca acgtctctgc taaacaactg gcagccaaat
      241 cacggctatt ggttaaccaa ttgctgggtg aaaagtatac gaatagagtg taccttcgca
      301 ctattcaaca gcaatgatag gcgctcacct gacaacgcgg taaactagtt attcacgcta
      361 actataatgg tttaatgatg gataacatgc agactgaagc acaaccgaca cggacccgga
      421 tcctcaatgc tgccagagag attttttcag aaaatggatt tcacagtgcc tcgatgaaag
      481 ccatctgtaa atcttgcgcc attagtcctg ggacgctcta tcaccatttc atctccaaag
      541 aagccttgat tcaggcgatt atcttacagg accaggagag ggcgctggct cgtttccggg
      601 aaccgattga agggattcat tttgttgact atatggtcga gtctattgtc tctctcaccc
      661 atgaagcctt tgggcaacgg gcgctggtgg tcgaaattat ggcggaaggg atgcgtaacc
      721 cacaggtcgc cgccatgctt aaaaataagc atatgacgat cactgaattt gttgcccagc
      781 ggatgcgtga tgcccagcaa aaaggcgaga taagcccaga catcaacacg gcaatgactt
      841 cacgtttact gctggatctg acctacggtg tactggccga tatcgaagcg gaagacctgg
      901 cgcgtgaagc gtcgtttgct cagggattac gcgcgatgat tggcggtata ttaaccgcat
      961 cctgatcctc tcatttcgcg ggctggtgat aactgtgccc gcgtttcata tcgtaatttc
     1021 tctgtgcaaa aattatcctc cccgtgttcg gagaattccc ccccaaaata ttcaccgtag
     1081 ccatatgtca tgagagttta tcgttcccaa cactctcgaa cgaacgttcg attgcttatt
     1141 ttatggcttc tgtcaacgct gttttaaaga ttaatgcgat ccatatcacg ctgtgggtat
     1201 tgcagttttt ggttttttga tcgcggtgtc agttcttttt atttccattt ctcttccatg
     1261 ggtttctcac agataactgt gtgcaacaca gaattggtta actaatcaga ttaaaggttg
     1321 accagtattt ttatcttaat gaggagtccc ttatgttacg tcctgtagaa accccaaccc
     1381 gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa aactgtggaa
     1441 ttgatcagcg ttggtgcgaa agcgcgttac aagaaagccg ggcaattgca gtgccaggca
     1501 gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgtgggcaac gtctggtatc
     1561 agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg cgtttcgatg
     1621 cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag catcagggcg
     1681 gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta
     1741 tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga atggtgatta
     1801 ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac tatgccggga
     1861 tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat atcaccgtgg
     1921 tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg
     1981 gtgatatcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca
     2041 ccagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa ggttatctct
     2101 atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg ctgcgcgtcg
     2161 gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa ccgttctact
     2221 ttactggctt tggtcgtcat gaagatgcgg atttgcgagg caaaggattc gataacgtgc
     2281 tgatggtgca cgatcacgca ttaatggact ggattggggc caactcctac cgtacctcgc
     2341 attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc gtggtgattg
     2401 atgaaactgc agctgtcggc tttaacctct ctttaggcat tggtttcgaa gcgggcaaca
     2461 agccgaaaga actgtacagc gaagaggctg tcaacgggga aactcagcag gcgcacttac
     2521 aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta
     2581 ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg ccactggcgg
     2641 aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg
     2701 acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac cgttattacg
     2761 gttggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa aaagaactgc
     2821 tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc gtggatacgt
     2881 tagccggcct gcactcaatg tacaccgaca tgtggagtga agagtatcag tgtgcatggc
     2941 tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga
     3001 atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac aattgaccta
     3061 catgggcctc gggctttgct acagcctggt gaatattcct tatggttcac ttgctaccgc
     3121 gatgacccaa caaccacaat ctcgcgcccg tctgggcgcg gctcgtggga ttgccgcttc
     3181 attgaccttt gtatgcctgg catttctgat aggaccgagc attaagaact ccagcccgga
     3241 agagatggtg tcggtatacc atttctggac gattgtgctg gtgattgccg gaatggtgct
     3301 ttacttcatc tgcttcaaat cgacgcgtga aaatgtggta cgtatcgtgg cgcagccgtc
     3361 attgaagatc agtttgcaaa ccctgaaacg gaatcgcccg ctgtttatgt tgtgcatcgg
     3421 tgcgctgtgt gtgctgattt cgaccttcgc ggtcagcgcc tcgtcgttgt tctacgtgcg
     3481 ctatgtgtta aatgataccg ggctgttcac tgtgctggta ctggtgcaaa acctggttgg
     3541 tactgtggca tcggcaccgc ttgtgccggg gatggtcgcg aggatcggta aaaagaatac
     3601 cttcctgatt ggcgctttgc tgggaacctg cggttatctg ctattcttct gggtttccgt
     3661 ctggtcactg ccggtggcgt tggttgcgtt ggccatcgct tcaattggtc agggcgttac
     3721 tatgactgtg atgtgggcac tggaagctga taccgtagaa tacggtgaat acctgaccgg
     3781 cgtgcgaatt gaagggctca cctattcact attctcattt acccgtaaat gcggtcaggc
     3841 aatcggaggt tcaattcctg cctttatttt gggattaagc ggatatatcg ccaatcaggt
     3901 gtaaacgccg gaagttatta tgggcatccg cacatcaatt gccttagtac cttgcggatt
     3961 tatgctactg gcattcgtta ttatctggtt ttatccgctc acggataaaa aattcaaaga
     4021 aatcgtggtt gaaattgata atcgtaaaaa agtgcagcag caattaatca gcgatatcac
     4081 taattaatat tcaataaaaa taatcagaac atcaaaggtg caactatgag aaaaatagtg
     4141 gccatggccg ttatttgcct gacggctgcc tctggcctta cctctgctta cgcggcgcaa
     4201 ctggctgacg atgaagcggg actacgcatc agactgaaaa acgaattgcg cagggctgat
     4261 aagcccagtg ctggcgcggg aagagatatt tacgcatggg tacagggagg attgctcgat
     4321 ttcaatagtg gttattattc caatattgtt ggcgttgaag gcggagcgta ttatgtttat
     4381 aaattaggtg ctcgtgctga tatgagtacc cggtggtatc ttgatggtga taaaagtttt
     4441 ggctttgccc tgggggcagt aaaaataaaa cccagtgaaa atagcctgct taaattaggt
     4501 cgcttcggga cggattatag ttatggtagc ttaccttatc gtattccgtt aatggttggc
     4561 agttcgcaac gtacattacc gacagtttct gaaggagcat taggttattg ggctttaacg
     4621 ccaaatattg atctgtgggg aatgtggcgt tcacgagtat ttttatggac tgattcaaca
     4681 accggtattc gtgatgaagg ggtgtataac agccagacgg gaaaatacga taaacatcgc
     4741 gcacgttctt ttttagccgc cagttggtat gatgatacca gtcgctattc tctgggggca
     4801 tcggtacaga aagatgtttc caatcagata caaagtattc tcgagaaaag cataccgctc
     4861 gacccgaatt atacgttgaa aggggagttg ctcggctttt acgcgcagct cgaaggttta
     4921 agtcgtaata ccagccagcc caatgaaacg gcgttggtta gtggacaatt gacctggaat
     4981 gcgccgtggg gaagcgtatt tggcagtggt ggttatttgc gccatgcaat gaatggtgcc
     5041 gtggtggata ccgacattgg ctatcccttt tcattaagtc ttgatcgtaa ccgtgaagga
     5101 atgcagtcct ggcaattagg tgccaactac cgtgtaactc cgcaatttac gctgacattt
     5161 gcaccgattg tgactcgcgg ctatgaatcc agtaaacgag atgtgcggat tgaaggcgcg
     5221 ggtatcttag gtggtatgaa ctatcgggtc agcgaagggc cgttacaagg gatgaatttc
     5281 tttcttgctg ccgataaagg gcgggaaaag cgcgatggca gtacgctggg cgatcgcctg
     5341 aattactggg atgtgaaaat gagtattcag tatgacttta tgctgaagta agaaataacg
     5401 ccggagagaa aaacctccgg cgtttcagat tgttgacaaa gtgcgcgttt tttatgccgg
     5461 atgcggcgta aacgccttat ccagcctaca aaaactcata aattcaaagt gttgcaggaa
     5521 aaggtaggcc tgataagcgt agcgcatcag gcaatctctg gtttgttttc agatgaaaac
     5581 gccggagtga aaattctccg gcgttttggc cgtgaattac tgctgcggaa ttgccggtac
     5641 agccggaacg ttaagagctg gcatcgcaaa catgccaaca aaatcttcta acgacatttt
     5701 ctgcccattt aacgttatct gaccgttagc atattgcagg ctggtggtga tggtattgtc
     5761 ctgcaaggtg gtcagacgga acatctgccc cattgctgat gcaccttcaa cttgctgttt
     5821 cgccagtttt ttcgcttgat cttcctgata accttccagc ttcgctacct gagtcatcaa
     5881 ctcagttgcc atatccaccg gaatggtcag tttcgcatcc agagatttaa ccgaacgatc
     5941 tacttcctgc gccagcgttt gcggcgcttc tttagtcgtt gccggatctt tcaggaacag
     6001 cgacagattc agcgcacttt caccctgact gtttttccag cttagcggcg cgatagtaat
     6061 caccggatcg cctttcaaca tcagcggcag ggcgctaaag aaggcttccg tcactttctc
     6121 ctgataaagt tcgggattgt tggcaatttc tggctgtgcc agcagcgcct gagtttgcgc
     6181 gttatattgc tggctaaact gatgccaggc ttcaccatca atctggccga cttttaaagt
     6241 cagcttgccg ctgcccagat cctgattctg taccttcagg ctgtttagcg agtaatccag
     6301 ttggctattg atcgttttac cgtcattgac cagatccgat ttaccgctga tctccatgcc
     6361 ttccagcagt gccagttctt tgccttccac tgaaatggtc attttttcca gtgacagttt
     6421 ttggtttcct acgcgctcac caaaacttcc cagcgtgctg gaaccgtcgg ttttcagatt
     6481 attaaaggtc aactgcactt tctggttgta ttcgttaact gcgtctatcc gaccactttg
     6541 cgcctctccg gaaagggaga tggctttgcc gtctctgtca gcatttaact ggaactcgcc
     6601 gccgctaaag gcgacttttt catccttttg ctcgtaattc agtggattga gcgaaatatc
     6661 ggaactggaa tcaccgctgt aaccaatgcg cgagttaatc tcaaaaggcg tttcaccttt
     6721 tgccatatca aacagtggtt tgcttacttc gttattaacc agcgtggttt gaattgatgc
     6781 catcgacggg atcaggttca gttttttaag ctgggcaagc gggaagggac catgatcaac
     6841 cgattcgttg aagatgacgc tctgaccgct tttaatccac ggattttctt tcccggcaat
     6901 gggtttcacc aacagttgca actggctgct gaatacgccg cgatgatagt tttgataact
     6961 cacttccagg ttggattcag gagctgtcag tttgagttgc gcgttcgcct gcgcgaccat
     7021 gtcttcgaga tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg tccagactac
     7081 gcctagcgca acaatgacgc ctaccgctac cagcgattta ttcataatga ttatccataa
     7141 aatgaaatca ggcggactgg ccgcctgaag gtgttataag cctttaataa gcttagcaag
     7201 agatgttaat tttttcagta agctcttaca gcttgttgta aacacgcgct aaacggccgt
     7261 ggcctttgac agtcaccggt gattcgttgg cggcaataaa cgctgattca cccggtttaa
     7321 gctgtaactg ctgagaacct ttccacaacg ttgcatcgcc ttcgacgcag aacaaaatgg
     7381 cggcactctg ctggctgatg gtggtttctt tatcactaag gtcatgcagc gagaaggcaa
     7441 aatcatccac aggaatcggg aagtccagtt ctgcaccttg tttcaccggc tgggtcaaca
     7501 actggttagc cggtttggct tcgaatttca cattggcaac cagttccgga atatcaatgt
     7561 atttaggcgt cagacctgca cgcagcacgt tatcggagtt cgccatcact tccagcgcca
     7621 cgccttgcag gtaagcgtgc ggtgtttcag cgaacaggaa catcgcttcg ccaggattca
     7681 atttcaccac attcagtaac agcggggaga acaggccgct gtcttccggg taaaattcag
     7741 aaattaaacg aatcgtttgc cacggttcac cctgctgact gtcgagcgcc gattttaaaa
     7801 tcgccagcgc gcgggatttt tcttcaccct gcatattcaa caggttggcg aacagttcgc
     7861 ttaaacgttc ggcatgtggc tgttgtaaaa agtgagcaat cgccggatgt gcaccggcga
     7921 ccggctggag tagggagaca atctcggaaa attcacgaaa cgcgttcatc gcaaggaaag
     7981 gcgtcagcgc aaaaaccagc tccggcttgt ggttaggatc tttatagtta cgctcggcgg
     8041 catccatcgg gatacctgcg gcattttctt tggcaaaacc gatttcagaa ttgtgtttgt
     8101 ttggatgaac ctgaatggag agtggctgtg ctgcgcataa tactttgaac aggaaaggca
     8161 gttcgccaaa gcgtttggca acggcctctc cgagcagagt cgatttatca ctctcaatca
     8221 catcacgcag tgaaacgata tctccggcgg cattctgcac tcgtgaactg cttttcggat
     8281 gtgcgcccat ccacagctcg gccatcggct ggctggacgg attttccata ccataaagtt
     8341 cagtcaacgc cgttttgcta ccccaggcat agttttgcac tgagttaatg agtttttgca
     8401 tgatcaatcc ctgttttaat gtggaaatta atcccgctat taaagcaaga atcctacggg
     8461 aagtaacctg gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa aactgtgtag
     8521 gatattgtta ctcgctttta acagggcaac ggaacacccg cccagagcat aaccaaacca
     8581 ggcagtaagt gagagaacaa tgtcaaacaa accctttcat tatcagtctc cttttccact
     8641 caaaaaaaat gatactgagt attacctgct aaccagtgaa cacgttagcg tatctgaatt
     8701 tgaagggcag gagattttga aagtcgcacc cgaagcgtta actctgttgg cgcgtcaggc
     8761 gtttcatgat gcgtcgttta tgctgcgtcc ggctcaccaa caacaggtgg ccgacattct
     8821 gcgtgacccg gaggccagcg aaaatgataa atatgtggcg ctgcaattcc tgcgtaactc
     8881 cgacatcgcg gcgaaaggcg ttctgccaac ctgtcaggat accggcaccg cgattattgt
     8941 tggtaaaaaa gggcagcgtg tatggaccgg tggtggtgat gaagcggcgc tggcgcgcgg
     9001 tgtctataac acttatatcg aagataatct gcgctactcg caaaacgcgc cgctggatat
     9061 gtataaagaa gtgaataccg gcaccaatct gccagcgcag atcgatcttt atgccgttga
     9121 tggcgacgaa tacaaattcc tctgtatcgc aaaaggtggt ggttcggcaa acaagacgta
     9181 tctctatcag gaaaccaaag cgttactgac gccggggaaa ctgaaaaatt acctggttga
     9241 gaagatgcgc acgctgggta ccgcggcctg tcctccgtat catattgcgt tcgttattgg
     9301 tggaacttct gcagaaacga accttaaaac ggtgaaactg gcttccgcga aatactatga
     9361 tgaactgcca acggaaggga atgagcacgg tcaggcgttc cgcgatgtgg aactggaaaa
     9421 agaattactg atcgaagcgc aaaatctcgg tctgggtgcg cagtttggtg gtaaatactt
     9481 cgctcacgac atccgcgtga ttcgcctgcc acgtcacggc gcatcctgtc cggttggtat
     9541 gggtgtttcc tgctctgctg accgtaatat caaagcgaag atcaaccgtc aggggatctg
     9601 gatcgaaaaa ctggaacata atccaggtaa atatatccct gaagagctgc gtaaagcggg
     9661 agaaggcgaa gcggtgcgcg ttgaccttaa ccgtccgatg aaagagatcc tcgcacagtt
     9721 gtcgcagtat cccgtttcta cacgcttatc gcttaacggc acgattatcg ttggtcgtga
     9781 tattgctcac gccaaactga aagagcggat ggataacggt gaagggctgc cgcaatacat
     9841 taaagatcat ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt atgcctccgg
     9901 ttctcttggc ccaacgaccg ccggacggat ggattcttat gtcgatcaac tgcaagcgca
     9961 gggcggaagt atgatcatgc tggcgaaagg caaccgcagc cagcaggtga cggatgcctg
    10021 taaaaaacac ggcggcttct accttggcag tatcggtggt ccggccgctg tattggcgca
    10081 gggaagtatt aagagcctgg aatgtgttga atatccggaa ctgggaatgg aagccatctg
    10141 gaaaattgaa gtggaagatt tcccggcgtt tatccttgtg gatgataaag gaaatgactt
    10201 cttccagcag atacaactca cacagtgcac tcgctgtgtg aaataaacag agccgccctt
    10261 cggggcggtt tttttacatg gcacgaaaga ccaaacattt gttatcaaat agtaaataat
    10321 gagtgagcta aaagttgctt aacgaaagca aaacagaaag aaaaaattaa tcaggtgagg
    10381 agcaggccat gaatacagta cgcagcgaaa aagattcgat gggggcgatt gatgtcccgg
    10441 cagataagct gtggggcgca caaactcaac gctcgctgga gcatttccgc atttcgacgg
    10501 agaaaatgcc cacctcactg attcatgcgc tggcgctaac caagcgcgca gcggcaaaag
    10561 ttaatgaaga tttagacttg ttgtctgaag agaaagcgag cgccattcgg caggcggcgg
    10621 atgaagtact ggcaggacag catgacgacg aattcccgct ggctatctgg cagaccggct
    10681 ccggcacgca aagtaacatg aacatgaacg aagtgctggc taaccgggcc agtgaattac
    10741 tcggcggtgt gcgcgggatg gaacgtaaag ttcaccctaa cgacgacgtg aacaaaagcc
    10801 aaagttccaa cgatgtcttt ccgacggcga tgcacgttgc ggcgctgctg gcactgcgca
    10861 agcaactcat tccgcagctt aaaaccctga cacagacgct gagtgaaaaa tcgcgtgcat
    10921 ttgccgatat cgtcaaaatc ggtcgaaccc acttgcagga cgccacgccg ctaacactag
    10981 ggcaggagat ttccggctgg gtagcgatgc tcgagcataa tctcaaacat atcgaataca
    11041 gcctgcctca cgtagcggaa ctggctcttg gcggtacagc ggtgggtact ggactaaata
    11101 cccatccgga gtatgcgcgt cgcgtagcag atgaactggc agtcattacc tgtgcaccgt
    11161 ttgttaccgc gccgaacaaa tttgaagcgc ttgcgacctg tgatgccctg gttcaggcgc
    11221 acggcgcgtt gaaagggttg gctgcgtcac tgatgaaaat cgccaatgat gtccgctggc
    11281 tggcctctgg cccgcgctgc ggaattggtg aaatctcaat cccggaaaat gagccgggca
    11341 gctcaatcat gccggggaaa gtgaacccaa cccagtgtga agcattaacc atgctctgct
    11401 gtcaggtaat ggggaacgac gtggcgatca acatgggtgg cgcttccggt aactttgaac
    11461 tgaacgtctt ccgtccgatg gtgatccata atttcctgca atcggtgcgc ttgctggcag
    11521 atggcatgga aagttttaac aaacactgcg cagtgggtat tgaaccgaat cgtgagcgaa
    11581 tcaatcaatt actcaatgaa tcgctgatgc tggtgactgc gcttaacacc cacattggtt
    11641 atgacaaagc cgcggagatc gccaaaaaag cgcataaaga agggctgacc ttaaaagctg
    11701 cggcccttgc gctggggtat cttagcgaag ccgagtttga cagctgggta cggccagaac
    11761 agatggtcgg cagtatgaaa gccgggcgtt aatctgcaac atacaggtgc agccgtggaa
    11821 tgatcaaacg aagcggctgc gcctgaggtt tataacggtg ctgcacattg tcggcatcgt
    11881 aatttaacaa ctcaccaacg tccggcacgc ccgcgccatt atcccgatta atcagtgcaa
    11941 tcagtggtgt agggcaggcg tgttggactt gtttttgatc tcctttgtac cagacgcggg
    12001 caatcggctg caccttcact ggacgtttga tttttaactt cgcgttctgt ggcagggcag
    12061 cgatatcctg atactctcgc tccagttttc tttgccactc ctcgcgcgtc cacggtgcga
    12121 cactgcgtgg tgatttcagg cttttttcca gctgtgccag gacttcatca cgatgcaaat
    12181 tcttaatgat atgtttatta gcccaaccaa agcgtaaagt ggcggggtcg tgcagaacgg
    12241 tgagcgtgcg gtaagcatta agggtgatca gccccggcaa atgacgatgc acccattcaa
    12301 aacgtgccgc ggtggggagt tctgattcaa ccgtgacgat atgctcgaac gtggtcttga
    12361 gtttattgat gtgctgaata tgactgacca acgctgcttg cgaaaggtta tcgacctggt
    12421 aacacaacac gccaggcaga cggacagcgg ccttgctgct gcgattttcg gactgttgtt
    12481 gaataaataa atggcggaaa tgacgcaacg ccagcgactg cgcgtcgttg ccgagatgtt
    12541 gttttacctc aatacgatta agcggattat gctcatcctc tttttttacc tccggcaaag
    12601 agaacacgcg ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat gtagccagct
    12661 cttgttccat ctggcgaaag gtagtgttga gtcggtctac gagatcgtaa cgcgccataa
    12721 tattaaccac tttagttaca acatacttat tttatagcac agtcgtggtg acttgaccat
    12781 cgtgcgcgta gtgtcaggca gaggtaaatt gcgggatgtt atgccataac ggccagctaa
    12841 acgagaagcg agcaccaccc agttcgctga tgtcacaatt aaccgtaccg cccattgtca
    12901 gtgctattga gtggacaatt gccagcccca gcccgcagcc gccggttgag cgatcccggc
    12961 tgggatcgag gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg gcaatccctg
    13021 ggccatcatc ctcaacaatt aatgtcgctc tattccccga cagtagcagg ctggtttcaa
    13081 ccgttgaatg gcagtagcgc agggcgttat tgagcaaatt atccagcacg cgctccatta
    13141 agcgcatatc caacgccgca taatggcctt gcatgagcgt ttttatccgt accgttttat
    13201 cgggcgttac tgcctgaata tctgccagat gcgttgacag ccacaacggc aggtctggtt
    13261 cgctaagatg aagctcgttt tgtgggcgat cgagtcgggc ataagtcagc agctcttcaa
    13321 ttaaagcttc aagttgactg atatcacgat tcaacgcctg ggattcggcg gcgctcaggt
    13381 tatcgctcat ctccagtcga taacgcaggc gcactaacgg tgttcgcagt tcgtgagcga
    13441 taccgtcaat aagctgtttt ttgctggcaa ttaaggcgtt gatattgtcc gccatctggt
    13501 taaatgcgat accaagtcgt tcaaagctcg aaccctcatc aaagtggata cgttcattga
    13561 gatgcccatc gccaaatcgt tgcgccgctg cttccagttt taacatatcc tgccagtgcg
    13621 gacgcatcca gataaacacc ggaaaggcga gggaaatagc aataaaagcg atcagggcga
    13681 tatccagcaa tcgcatttga tggaggtaat aaagataagg aacaggacca actgccagca
    13741 cgtagtggct gcgcgggata cgctgcaaaa acgtgtactg atcgtccagg gcgacaattt
    13801 cgccgccacg cagtcggtgc atggaaatat catcaagatg gtatttactc agtggctcga
    13861 cacgcagatc gaaagagaga tttaaatcca tctctttcag cgtcttaccc cagtcgtgtg
    13921 gggggatctc tcgcaattcg ctgcgcatca gatacagcga actgttcatc aaatcatcca
    13981 gcgactgttt gcccgcgcgt tcggcggtaa atttgtacac cagcccaacc agcagagaca
    14041 tcacaaggaa gcagacaaac aataacaggt aaaactggat aaacaatttt ttcattggca
    14101 tcgcccgcaa cagaatacta ttcgtattaa ttcacgactg tggagcaatg taaaccgcca
    14161 actggataag accttgtttg gaagataatg tccggaacag acggatgtat ccccggtgta
    14221 ttcatattca gtaaattcaa ttttccaccg gggcttgttt attatgctaa ggtttaattt
    14281 aacgtgacgc tttgttgttc tttagtctct ctatccttaa gaagacgggc tgatattaca
    14341 ccggacatta ttgaaccatt cacattaagc gctgttcttg ccatatcaac gatcggctct
    14401 atggcaataa aaataactac taatgcgaca gggaaattaa gtgttgaaag gacaatcagc
    14461 gccgcgaatg tcccaccact acctacgcct gctaccccta ttgagccaag cgcgatggca
    14521 ggtaaaagag ttgcgaggaa ttgcaatgag aagggatcga tacccattgt tggagcaatc
    14581 atcgccacca tcacggcagg ataaatccca gcgcagccat tttgtcccat gctggaacca
    14641 aaagacgctg caatattagc aatcgtattt ggaaccccca atttttccaa tgcagaaata
    14701 gccagaggaa ttgaagccgc actactgcga gaaacaaacg caaaggtaag tacttgccag
    14761 acggtcttgt aatagttaac ggggttttta cctattaaca gcaaaatgcc cgagtgaaca
    14821 ataaacatca caaaaacggc cagataacag gcgacgatga acccgagcaa attggtaaac
    14881 tgtgaaagct gatacgttga gaagactttc gccattagtg ccattacccc ataaggcgtc
    14941 agcgcaatga caatacggac cattttcatt acccatattt gaatcgcgtt gatccccgcg
    15001 ctcaattttt cgccctctgc tggtgcatct ttctttactt ttaacagcgc aataccagca
    15061 agaattgtaa agatgacgat gccaataacc gaaacacttc ttgccccggt cagatcaagg
    15121 aagaggttgg ttgggatcaa tgacgtaatg agtcgcggta aagataccgc agctgttttc
    15181 gagacgtcat cgctggttaa agaggattgc atatgaccaa aggcgctggc atcaaggcca
    15241 attgcactgg caatagccag gccaactaaa cctgcaatca tgactaaaca caacatacag
    15301 gcaacaatag ttaaagacat cttgccaata ccagcgctat tttcgagctt attaatcgct
    15361 gaaagaatcg aaatgaatat gagtggaacg gcaacaagtt ttaataaatt aacataaccg
    15421 ttaccagcaa tattatacca ttcgataacg gtttcactgc ttgtctcagg aatgaaatgc
    15481 agggagatac caatgatagc ccccaggaaa atcgcgataa aaacactttt ggaaagggat
    15541 tcgctctttt gatccgtttt gaaaatcccc catgagcaga tcaaaaaaag cacaatatat
    15601 gtgaaagttg taaccgtttt ttaagcctct tgcctcattc agcacctgaa atgccggaaa
    15661 gagtttgttg taaaaaatct gcgccataag acgcagggac gaggcgatta tagagaaatt
    15721 ttttctcatt ttcatatcaa atgtaggggg tttggaattt gaataagctc ttattaattg
    15781 aattcgttaa ttgttctctt tttcagatga acaaatatta gtcatctgat tgtttattgt
    15841 cactatccca tgcatgaggc gcaaaaagat agcctttgtt acgcacagtt ttaatgcgat
    15901 aaggttctgc ggcgttatcg agcagttttt ttcttaaccg cgaaatagcc acgtccacgc
    15961 tacgatccag tccgtcataa ctgacgccgc gtaaattttt cagcaatgca tcgcggtcca
    16021 tgatttgccc ggcatgcgta gctagttccc acaataattc gaaatcagct gtcgagagcg
    16081 agatttcagt gttagccagg gtgactacgc ggttgatggg gtcgatagtt aacgtgccga
    16141 aatgcagggc tttgtaggga gtaagagacg tttcctgaag acctttggtc agtgtggctt
    16201 gctcattctg acgcaaatgc aaacgtaaac gcgctagcaa aacagcaggg ggcgtcgttt
    16261 tgagaatata gtcgcaggca cccatttcca gtgccaggat gtggttcata tcgctatcga
    16321 gagaggttag aagaacaatc ggtccagacc actttgcgcg taaatcacga caaatggtca
    16381 tgccgtcctt gcctggtagc atgatgtcga gtaacaccaa atccggattt tctcgcaaaa
    16441 tggtttcttc ggcctggtcg ccgcgcggtt ctacggtaac ctgcatatca tgttttgcca
    16501 ggtacgcggc aatcagtgaa ccgacttccg catcatcttc cacaaatacg atagtgttca
    16561 taacattcac ggtagatata aaaacgtcac aatacaccgc gctgttttta cttactatca
    16621 atcgtttcta aacaagtttt catcaggtat tctactgatg acttgttatg gaagtgttaa
    16681 ggtaaaaaga tggggctggt aatcaaagcc gcgctcggtg cgctggtagt gctgttgatt
    16741 ggtgttttag caaaaacgaa aaattattat atcgccgggc tgattccact tttcccgacc
    16801 tttgcgctta tcgcgcatta tattgttgcc agcgaacgcg gcattgaagc cttacgcgca
    16861 accatcattt ttagtatgtg gtcgattatt ccctattttg tttacctggt gtcgctgtgg
    16921 tatttcaccg gaatgatgcg cttgcccgcc gcttttgttg gttctgttgc gtgttggggg
    16981 ataagcgcat gggtgttgat tatatgctgg ataaagctgc attaacgcag atgacgaccg
    17041 ccatcaagtg ggaaactgcg tccggtgaca aagcaactgg taagtaagta atcgaccagg
    17101 tcgatcactt ctttctcgcc aggcgcggtt ttcatcagtg atttattcag cgcctgttgt
    17161 cgatattcgg catcatcatg ttcattaaac aggatcagcg atggcgcaat agaattcact
    17221 ttcacttccg gtgccagctt gcgggcaaac gagcgggtca tattatccag tgccgctttg
    17281 cttgcagcat acgcaacatg tttgtcgcta ccgcgctcca ccacataatc ggtaaagtga
    17341 atgatatcgc tggcggcgtg tccgtgccca cgcagtaatc tttccagcgc atggttgagc
    17401 aggtatgggg tattaacgtg gatctgcatc atgcaagcca gtacgtcggc cagtggcgca
    17461 cccggttttt ccgcaatcca cgcactggcg ttatgcaaaa tagcacgcag accatgggtg
    17521 gtttttagta cttcatcggc aaacgccatc acaccgtcgt tggtcgaaaa atcagcctga
    17581 atacactgcg cacctgcatt aatcagtcca tcaatggctg gatagtgtgt ccgatagctg
    17641 acaatcaccg gttgcttttg attaatgaaa tgccatgcga gggcgaggcc gatgcgacga
    17701 cctccgccag taattaatat tggcaagggc tgggttttac ccatcgttat ctcctttgct
    17761 atccaacgat gggatgaacg ttatcccacc agcatccagg tcgccggaac tgaggcaatt
    17821 aacagcatgc caatcagcac catttcctga cggttcagta cattatcatg tgtatgcgtt
    17881 ttgcgcgcat acaggaaaac cagcagcccc ggtgcataca gaacaacgga taacagcaag
    17941 tgcattggac cagaggcgta caataaccat aagccataaa tgcaggcacc gacacctacc
    18001 gctttatgca gtggacgggt tgcgattttc agcaagaacg cgccgaccag gaaatagggc
    18061 accagaatca tttctgaggc gatggtcagc aacgtgttgt aatcggaacc ggtcagccag
    18121 atgagtacca gacaaatttg cacgcagatg ttggtcaacc acagtgaggc ggatggcgca
    18181 gcttgtgcgt tctggcgcgc aaaaatgcgt gggaatgctt tatgagtggc agccaggaac
    18241 ggaacttctg ccgccatgat ggtccagctt aagtacgcgc cgcaaacgga aacgatcaaa
    18301 ccggcagcga tgatgatttc gccccatggt cccatcattt ccaccatcag accggccatt
    18361 gacgggttac gaatttcagc cagttcagga cgtgccacca cacccagcga aagcagcgtt
    18421 accagcaagt aaacgcccag agcggagaga accgccagca gtgtcgcttt gccaacatca
    18481 cgtttattac gcgcacgcgc agaaacaacc acggcacctt ccacaccaat gaaaacccac
    18541 agggtgatca gcatggtgtt tttcacctgt tcccaaacag gtacgccaag tgcaagtccg
    18601 gtgaagtcga gcttgaaggt atccagtttg aacatcatca tcgccagcac aacaaacaga
    18661 cccagcggca acaattttgc cagtgtcgcc accaggttaa tgctggcagc ggtttgcacc
    18721 ccgcgcagga tcaaaaagtg aacaatccac aataaagccg atgcaccgac tatcgattgc
    18781 caggtattac catcgccaaa caggcgcaat tccggcgtgt ccgtaaaaaa gcttaacgcg
    18841 gaaaaaacga tcaccagata ggagacgttg gcgatgaccg cgcacagcca gtatccccat
    18901 gcggaacaaa agccgattag ctcgccaaac ccttcgcggg cataggtaaa gataccgcca
    18961 tcaagttcgg gacgaatgcg cgtgaggatc agcatggcaa aggccagcaa taaaatgcca
    19021 gcgccagtaa taccccagcc gatgagcagt gctgccgggc tggcaactgc cgccatattt
    19081 tgcggcagac tgaaaacacc cgcgcccagc attgagctta ataccagcgc ggtgagtgcg
    19141 ctcagtccca gtttcttttc catcgatatc cagtgattaa aacgtgataa gaagaataaa
    19201 tattagggca aaacgcataa aaatggagtt tgccactgaa gcgcgggatt ttacgaagag
    19261 atgtgtctgc atgcaatggt cgcggggaaa tttgtgacta aaaatgcgaa aggcggcatc
    19321 aagccgcctt ataggagtaa ctgaatagtt atttatacag atctgcgctg acggtcaggt
    19381 tattaccacg tttctgccac tggcgggtga tgtggtaata cttagcacct ttcttcgcgg
    19441 cacgtttcgc aacctgatag gagacttcgg tcatgttgcc gtagttgcca gagaatttga
    19501 tgctgtcgaa cgggaccatc atcgctgctg tcgctttgtt cagttcttcg actttagtgc
    19561 catccgggag cgtgacggtg taacgcccgc cttttgatga ctgggtttca aagaagcgac
    19621 cgacttcaga acttggtgat gcggtagtcg caacacccgg gatctcaact ttcttcgcgg
    19681 cttcgccacc ggcagccaga gctgcacgtc ctgcttcgga atctgccggg atcacatccg
    19741 ggctctggac gatacgtttc ttagcatctt ttttatagat gaatgcagta atacgctggt
    19801 tgccgccctg gttggcatcg atttgacgaa cgatgtagaa agagtaggca cctttcgctt
    19861 ttgccgcttt ggtgatggca tcattgactt ctggctggct acgatagaag ccctggacgg
    19921 tgaccgtgtc aaacggttca atcagaacag cctgatcttt cggcagttcg acaacaccgt
    19981 taattacgcg attacttgtt tcttctgctt tttcagcatc ggctttatag aggtcagcga
    20041 ccacacgcca gttaccgctg ttaccaaaat cagaagtgtc gacaacataa aaagaggcgg
    20101 caccttcttt atctgcgcga cgagaaacgg ctttcaccgc ttcgccaata gcattaaaac
    20161 gaccggtaac cactacacgg tcaaaaggtt taaccgctgc cgcttgctcc ggtgtcagtt
    20221 ctgttgctgc gttaacggag aatgccgtag cagaaagcag tgccgacgcc aggagggtgt
    20281 tcttaagctt cataaaaata atccttcgcc ttgcgcaaac caggtactgg tattgttatt
    20341 aacgagaaac gtggctgatt attgcattta aacggtgtaa ctgtctgcgt catttttcat
    20401 atcacattcc ttaaaccaat tttaatcctg ctcaaatgac cgtctatgct taaaaaacag
    20461 ccgtatcagc atcattacta ctgaagcaac tgaattgtat aagttaattt aatgttaagt
    20521 agtgattcgt gccggggcga tgtctcgttt tacccgaccg tcgaagacaa ttatcagtct
    20581 ttatccggcg ttctaaggtg tttatcccac tatcacggct gaatcgttaa tattttgcga
    20641 gttcacgccg aaatactgat ttttggcgct agatcacagg cataattttc agtacgttat
    20701 agggcgtttg ttactaattt attttaacgg agtaacattt agctcgtaca tgagcagctt
    20761 gtgtggctcc tgacacaggc aaaccatcat caataaaacc gatggaaggg aatatcatgc
    20821 gaattggcat accaagagaa cggttaacca atgaaacccg tgttgcagca acgccaaaaa
    20881 cagtggaaca gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc gcgggtcaac
    20941 tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta gaagggaata
    21001 gcgtctggca gtcagagatc attctgaagg tcaatgcgcc gttagatgat gaaattgcgt
    21061 tactgaatcc cgggacaacg ctggtgagtt ttatctggcc tgcgcagaat ccggaattaa
    21121 tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat ggactctgtg ccgcgtatct
    21181 cacgcgcaca atcgctggac gcactaagct cgatggcgaa tatcgccggt tatcgcgcca
    21241 ttgttgaagc ggcacatgaa tttgggcgct tctttaccgg gcaaatcact gcggccggga
    21301 aagtgccacc ggcaaaagtg atggtgattg gtgccggtgt tgcaggtttg gccgccattg
    21361 gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga cacccgcccg gaagtgaaag
    21421 aacaagttca aagtatgggc gcggaattcc tcgagctgga ttttaaagag gaagcgggca
    21481 gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa atggaactct
    21541 ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt ccaggcaaac
    21601 cagcgccgaa gctaattacc cgtgaaatgg ttgactccat gaaggcgggc agtgtgattg
    21661 tcgacctggc agcccaaaac ggtggcaact gtgaatacat cgtgccgggt gaaatcttca
    21721 gtacggaaaa tggtgtcaaa gtgattggtt ataccgatct tccgggccgt ctgccgacgc
    21781 aatcctcaca gctttacggt actaacctcg ttaatctgct gaaactgttg tgcaaagaga
    21841 aagacggcaa tatcactgtt gattttgatg atgtggtgat tcgcggcgtg accgtgatcc
    21901 gtgcgggcga aattacctgg ccggcaccgc cgattcaggt atcagctcag ccgcaggcgg
    21961 cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg tgcctgctca ccgtggcgta
    22021 aatacgcgtt gatggcgctg gcaatcatcc tgttcggttg gctgtcaagc gttgcgccga
    22081 aagagtttct tgggcacttc accgttttcg cgctggcctg cgttgtcggt tattacgtgg
    22141 tgtggaatgt atcgcacgcg ctgcatacac cgttgatgtc ggttaccaac gcgatttcag
    22201 ggattattgt tgtcggagca ctgttgcaga ttggccaggg aggctgggtt agcttcctta
    22261 gttttatcgc ggtgcttata gccagcatta atattttcgg tggcttcacc gtgactcagc
    22321 gcatgctgaa aatgttccgc aaaaattaag gggtaacata tgtctggagg attagttaca
    22381 gctgcataca ttgttgccgc gatcctgttt atcttcagtc tggccggtct ttcgaaacat
    22441 gaaacgtctc gccagggtaa caacttcggt atcgccggga tggcgattgc gttaatcgca
    22501 accatttttg gaccggatac gggtaatgtt ggctggatct tgctggcgat ggtcattggt
    22561 ggggcaattg gtatccggct ggcgaagaaa gttgaaatga ccgaaatgcc agaactggtg
    22621 gcgatcctgc acagcttcgt gggtctggcg gcagtgctgg ttggctttaa cagttatctg
    22681 catcatgacg cgggaatggc accgattctg gtcaatattc acctgacgga agtgttcctc
    22741 ggtatcttca tcggggcggt aacgttcacg ggctcggtgg tggcgttcgg caaactgtgt
    22801 ggcaagattt cgtctaaacc gttgatgctg ccaaaccgtc acaaaatgaa cctggcggct
    22861 ctggtcgttt ccttcttgct gctgattgta tttgttcgca cggacagcgt cggcctgcaa
    22921 gtgctggcat tgctgataat gaccgccatt gcgctggtat tcggctggca tttagtcgcc
    22981 tccatcggtg gtgcagatat gccggtggtg gtgtcgatgc tgaactcgta ctccggctgg
    23041 gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc tgattgtgac cggtgcgctg
    23101 gtcggttctt cgggggctat cctttcttac attatgtgta aggcgatgaa ccgttccttt
    23161 atcagcgtta ttgcgggtgg tttcggcacc gacggctctt ctactggcga tgatcaggaa
    23221 gtgggtgagc accgcgaaat caccgcagaa gagacagcgg aactgctgaa aaactcccat
    23281 tcagtgatca ttactccggg gtacggcatg gcagtcgcgc aggcgcaata tcctgtcgct
    23341 gaaattaccg agaaactccg cgctcgtggt attaatgtgc gtttcggtat ccacccggtt
    23401 gcggggcgtt tgcctggaca tatgaacgta ttgctggctg aagcaaaagt accgtatgac
    23461 atcgtgctgg aaatggacga gatcaacgat gactttgctg ataccgatac cgtactggtg
    23521 attggtgcta acgatacggt taacccggcg gcgcaggatg atccgaagag tccgattgct
    23581 ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa acgttcgatg
    23641 aacactggct atgctggtgt gcaaaacccg ctgttcttca aggaaaacac ccacatgctg
    23701 tttggtgacg ccaaagccag cgtggatgca atcctgaaag ctctgtaacc ctgacggcct
    23761 ctgcggaggc cgtcactctt tattgagatc gcttaacaga acggcgatgc tttgacctcc
    23821 cgctgtttgt tcaagcgcaa ttttgacaat aattgtcaac ggcacggaaa gcagcatacc
    23881 caccggtcct aacaaccatc cccaaaaaat caacgacaaa aataccacca atgtcgaaag
    23941 ccccagccca cgtcccatga tgcgcggctc cagaatattg ccgaagacca gattaatcag
    24001 cagatatccc gccagcacca gcaacgcttc gtagaagcca ttaaacacca gtacctgagc
    24061 gatagggggg attgccgcga gaactgaacc aatattaggg atgtaattaa gcgcaaaggc
    24121 cagcaatccc cagacaaaag cgaagcgaac atcgagtgcg gcgagcatcg cccaggcgac
    24181 caggccggtg atgatgctga tggctgtttt cagcaccaga taatgagaaa cactgtcaat
    24241 cgcacgttga atcgccgcca tcccttcaac cggacgcgcc atcatttgct gaaattttcc
    24301 gggcaattgt ggcacttcga gcagcataaa cagcaccgtc agcagcaata aaaatattga
    24361 tgacatggca ttagataact gcgtcaataa gttggtgagc aacgtcatcg ccgcgttcgg
    24421 atcaatgtaa tgcgccagtt ggtcaactga gacgtcaatc cctacgcgtt gcaacaacgg
    24481 ctcaagagct tgcagcggcg tcataataga gttgcgatat tgcggtaacg tccgcgtcaa
    24541 ctcgttgagc gcggaaccca gataagctaa tagcaacacc atcgccatca cgatgattgt
    24601 catcaaaatc gacactgcca gtacacgcgg cacacgccag cggaccatat gttgcaccag
    24661 cgggttaaga ataacagcaa taaataatgc gagaataaac ggcacgatga tctcggcggc
    24721 aaaacggata ccgcagagaa taatgaccag cattcccaac ataatgacga tttttaggcc
    24781 attgagcgtg atgatcggct ttgccatgct tgtccccgtt tttcttcctt tgctaataat
    24841 aatgtctttt tcgctccgtc atcataacga aaatcaaacc gctcaggtaa agaagtgaaa
    24901 atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact accgaggaca
    24961 attatcatct gcgatgacga gaagcaacac tgcggataat tgtaatatta tggacaattt
    25021 gttcaggacg ttattttcta catttaccca tcttcgtacc tcatccacta tcttgctcgt
    25081 aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc ttgccctatg
    25141 cgcagggctt gaccacttta ctttggtttc gctgaattaa gcgaaaatta aaatagttct
    25201 cttgcaggag aaggacaatg tatatttatt ggattttatt aggtctggct attgctacag
    25261 aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat ggcggcttta
    25321 ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc gttaaaaaaa
    25381 tcgccttagg cgtagcttat gcgctgtggg aaggtatcgg tattttattt attaccttgt
    25441 ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg ttaaccaccc
    25501 tggtcgccgg gatagtgttg atcaaatcag gtacccgtaa agcgcgtaaa cctgaactgg
    25561 aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt ggcaatcgtg
    25621 ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg caaaatattt
    25681 ggcttgctct ccctggcggc ggtgctggcg gcctttagtg cgctttctca ggccgttaaa
    25741 gggatcgact tgtctgtcgt ttatgcattg tggggcgggt ttggtattgc cgccacgtta
    25801 gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat tggcctggtc
    25861 ttgctgttgg ctggaatgat catggtgaaa cttgcctgat gaagacgctg cccgcgctgt
    25921 cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg aaaaccggta
    25981 acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc cgaactttgc
    26041 accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc gctgtcaccc
    26101 ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg cccagccagt cacttcacag
    26161 ttttgatgac tgtataacgt atcgagatga tcttcagggt atcccgcctg agtcacttta
    26221 cgaccagccg attttaatgc ggcagtaagc gcggctttat ctccctcaaa taacggcaac
    26281 ggtgtaatgc cagaaggggg attacgtagc acaatcaatc cgaagtccca cggtgcagct
    26341 gcgggaggaa caatccaacc atccccatct gcttttaacc gctttcccag tgtcggatca
    26401 acgcggcctt ctatgtcgtg gatctcatag cgccaaagac ctttatttga cacaaaacgc
    26461 agcgctaccg ccttatccgc tttaccctta ggcggcgtca ataaacagtg tcctgccgtt
    26521 aatgccagat tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc cgtttccagt
    26581 tgcccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg atcatcatga
    26641 ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg tttatctgca
    26701 aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt tgtacgcata
    26761 tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata ctatagacgg
    26821 gacgggatga aagtgggagt aaaatcagat aactacagtc aggaaagata aaaacgggcg
    26881 gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata acggcccacc
    26941 atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt aattttcagg
    27001 cgcgaggggg cgcgccagca ttactgttga aaacttacgc tgcgggttgt gcagcaggtt
    27061 ttgccggttg ctgatggccg tgtttcccgg cgtgtttctt cgctgcctgc gctttttgtt
    27121 caggggcttt ctgttcagct ttcgtatttt tatgatgctt tttagccgcc tgcgctttct
    27181 gggcaggtgc tgctttatgc tgttttttat gatgtgtagt tttcgctggc gctgctttgg
    27241 tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg gcagaagaca
    27301 gacccatagc agcggcaaca accagagcta atactttttt cattgtcata ccctcaattt
    27361 gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg ggaatggcag
    27421 acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga ataaattaca
    27481 gcgttgataa taacgtattg tgacttaagg aaaatttagc taccaataat agtagtcttg
    27541 atcgggttaa ctattatact acttacaaag cggtagaata actgcgcatc agtaataaat
    27601 gacacacagc aaaatgaatc cgtttatttg ggtacttata atcctgatga cgctagatgc
    27661 gctgcgggaa ttggctggcg cttcgtctat tttaggatgg ctattaacgt tggtttgagc
    27721 aggcaataag tccggacggg tatttaccgc agtccggact tatttttcag gcgtgcagac
    27781 gacgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca ataaatgcgc
    27841 cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg gcaatactcg
    27901 accccagata gtaactgaac agatacagcg aggaggcctg gcctttagcg cgttttgcgc
    27961 gggggccgat ccagctgctg gctactgagt gggctgcgaa gaatccggct gagaagagta
    28021 acattccggc aaagatcagc cacagcgagc taaataaggt catcagtaaa ccaaacggca
    28081 taactcccgt cgaaaacaac atcaccggac cgcgcccata gcgggtggtc atggttccgg
    28141 ctttgggtga gctccatgta ccggtcaaat aagccagcga taataagcca accacggcct
    28201 gactgacatg ctagggggaa agcatcaacc gatagccgat gtaattaaac agcgtgacga
    28261 acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg tcacgccagt
    28321 gcagacgaaa gttgataaat aacgtcttag ggcgcagcga agtcgggcaa aaatggcgtg
    28381 attcagggag gattttccag aacatcaacg ccgaggccag cgcgaaacaa ccgattgccg
    28441 ccagagcaat tcgccagttg aaaaagtccg tgaagacacc actaattaag cgtccgctca
    28501 tgccgccaat tgagttgccg ctgatataca accccatcga aaaggccacg aaactggggt
    28561 ggatttcctc gctaagataa gtcatgccaa cagctgccac gccacttaac gaaagcccaa
    28621 tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa agtaacgtac
    28681 aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg gcatcggata
    28741 gcgggccagt aaatagcaaa ccgatagcca acatcgccgt ggaaatggac agtgaaatac
    28801 tactgttcgc gggcgttaag ccaaactcct gcgaaagcac cggaaggata ggctgcacac
    28861 aatagagaag tgcaaaggtt gccagtccgg cagagaacag cgccagggtg acgcgcataa
    28921 attgcggcgt accgcgttta ataaattgat ttggctgaga aatgctttgc ttgtcagtgt
    28981 cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct tgctaaatat
    29041 gcctgtggat caggcttata catagggtag gaaaatcgaa ttgttctgtc taatatatta
    29101 ataatctcaa ataagatgtt ttaaatatga atattgaact tcgtcatctg cgttactttg
    29161 ttgctgttgc ggaagagctg cattttgggc gcgcggctgc ccgcctgaat atttcgcaac
    29221 cgccgctaag tcagcagatt caggcactgg agcaacaaat cggtgcccga ctgctggcac
    29281 gaactaatcg cagtgtattg ctgacggcag caggaaaaca gtttcttgca gatagtcggc
    29341 aaatcctgtc tatggtggat gacgctgccg ctcgcgctga aagactgcat cagggtgaag
    29401 cgggggagtt gcgtattggt tttacttcgt ccgctccttt tattcgggcg gtgtccgata
    29461 cgttatcgct gtttcgccgt gattatcctg atgtccattt acaaacccgc gaaatgaaca
    29521 ctcgcgagca aatcgctccg ctcattgaag gaacgctgga tatgggattg ctgcgtaaca
    29581 cagcgttacc ggagacgctt gaacacgcag tcatcgtcca tgaaccgctt atggcgatga
    29641 tcccgtacga tcatcccctg gcaaataatc cgagtgtaac actggctgaa ctggctgaac
    29701 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt
    29761 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    29821 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    29881 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    29941 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    30001 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    30061 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    30121 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    30181 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    30241 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    30301 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    30361 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    30421 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    30481 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    30541 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    30601 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    30661 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    30721 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    30781 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    30841 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    30901 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30961 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    31021 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    31081 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    31141 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    31201 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    31261 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    31321 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    31381 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    31441 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    31501 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    31561 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    31621 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    31681 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    31741 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    31801 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    31861 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    31921 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31981 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    32041 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    32101 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    32161 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc
    32221 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    32281 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    32341 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    32401 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    32461 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    32521 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    32581 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgcctagt
    32641 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    32701 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    32761 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    32821 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    32881 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    32941 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    33001 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    33061 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    33121 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    33181 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgt gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc
    36121 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    36181 acatagccca tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc
    36241 aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca
    36301 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    36361 tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    36421 ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca
    36481 tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat
    36541 tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg
    36601 ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt
    36661 cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc
    36721 aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta
    36781 gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg
    36841 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    36901 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36961 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    37021 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    37081 ccgccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag tttcgtattg
    37141 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    37201 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    37261 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    37321 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    37381 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    37441 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    37501 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    37561 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    37621 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    37681 aatggcagag tgaacgcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    37741 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    37801 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    37861 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    37921 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37981 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    38041 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    38101 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    38161 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    38221 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    38281 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    38341 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    38401 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    38461 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    38521 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    38581 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    38641 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    38701 atatcttcct gctcaacggt catcaggtct tccagcagaa tgtcggcata ttttgccgat
    38761 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    38821 tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag
    38881 atggactcca ttgccagacg gatggatgga cttgaagtag ttgactcaat gctacttaag
    38941 gtcctgatat gactgacatt aatcatgata acactgtata tccatccagt tctttctctg
    39001 ggtctcccta tcggtatcag caagtctaca cctcaagccc tgacacaagg ctgacacaaa
    39061 tcaatctact cagcgtagct aagcctcagt tagtccgcag agccaatggt cgatatacta
    39121 tccgcttcag actcaaggga cagaccacac cgtttctttc agtatcgacc cgcagcacag
    39181 acaggagagt agccacaatg cgtcagagag agcttgcagc caccgctaag gccttcatgt
    39241 tggataggcc tgaagtctca ctacaggagc ttacagagca tctccggtct atggctgagc
    39301 agttcctgac agacgctagt gacgattact ggaatggact tgaggtcgct actctggtcg
    39361 acgagaagtc caacctgaag gagctagccg ctacgcaagc cttgagtctg gaccagcaga
    39421 agggcataag gttagcgctg gaagtcctga ccgcagcaca gcagagagtc gacactggtg
    39481 atacctctgg tctgattaag ctgattgatg ataataacct tactgatgac tcaactatag
    39541 gcgactctac gtcaattctg aataatgagc agggggatag accggcagtt ttcacacagg
    39601 agcgccagtc atctgtagtt ttttcaagcc tagtgtcctc gctgctggct gagaaggtcc
    39661 agaccctgaa gacatccagc tacaaggacc tctcgtcctc actcaatact gtctcccggt
    39721 tcctgcctga agacatggac ctgatgtccc gctctgggtg gctggctgtc agggactcta
    39781 tgctggcttc tgaggtgaga ccatcgacaa tcaacaagct gctgaccaaa gcgaagatgt
    39841 gtctcgatta tggcctgatg aatgggcaac ttgagggccg caaccctatt gaacggatga
    39901 agctgaccaa ggatatcgac tccaagcgca gagcgttcac agacgaggag ctggagagac
    39961 ttctggtccg tgttgagtct g
//
LOCUS       DAJTYM010000028        39875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTYM010000028 DAJTYM010000000
VERSION     DAJTYM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(215..1099)
                     /gene="yghA"
                     /locus_tag="OO575_001477"
     CDS             complement(215..1099)
                     /gene="yghA"
                     /locus_tag="OO575_001477"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6664491.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            1290..1784
                     /locus_tag="OO575_001478"
     CDS             1290..1784
                     /locus_tag="OO575_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6664492.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(1824..2864)
                     /gene="gpr"
                     /locus_tag="OO575_001479"
     CDS             complement(1824..2864)
                     /gene="gpr"
                     /locus_tag="OO575_001479"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6664493.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            3021..3908
                     /locus_tag="OO575_001480"
     CDS             3021..3908
                     /locus_tag="OO575_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6664494.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            4027..4314
                     /locus_tag="OO575_001481"
     CDS             4027..4314
                     /locus_tag="OO575_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6664495.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            4503..5621
                     /gene="hybO"
                     /locus_tag="OO575_001482"
     CDS             4503..5621
                     /gene="hybO"
                     /locus_tag="OO575_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6664496.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            5624..6610
                     /gene="hybA"
                     /locus_tag="OO575_001483"
     CDS             5624..6610
                     /gene="hybA"
                     /locus_tag="OO575_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6664497.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            6600..7778
                     /gene="hybB"
                     /locus_tag="OO575_001484"
     CDS             6600..7778
                     /gene="hybB"
                     /locus_tag="OO575_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6664498.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            7775..9478
                     /gene="hybC"
                     /locus_tag="OO575_001485"
     CDS             7775..9478
                     /gene="hybC"
                     /locus_tag="OO575_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6664499.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            9478..9972
                     /locus_tag="OO575_001486"
     CDS             9478..9972
                     /locus_tag="OO575_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6664500.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            9965..10453
                     /gene="hybE"
                     /locus_tag="OO575_001487"
     CDS             9965..10453
                     /gene="hybE"
                     /locus_tag="OO575_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6664501.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            10446..10787
                     /gene="hypA"
                     /locus_tag="OO575_001488"
     CDS             10446..10787
                     /gene="hypA"
                     /locus_tag="OO575_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6664502.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            10921..11169
                     /gene="hybG"
                     /locus_tag="OO575_001489"
     CDS             10921..11169
                     /gene="hybG"
                     /locus_tag="OO575_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6664503.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(11292..12158)
                     /gene="yghU"
                     /locus_tag="OO575_001490"
     CDS             complement(11292..12158)
                     /gene="yghU"
                     /locus_tag="OO575_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6664504.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            12363..14222
                     /gene="gss"
                     /locus_tag="OO575_001491"
     CDS             12363..14222
                     /gene="gss"
                     /locus_tag="OO575_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6664505.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            14514..16013
                     /gene="pitB"
                     /locus_tag="OO575_001492"
     CDS             14514..16013
                     /gene="pitB"
                     /locus_tag="OO575_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6664506.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(16062..16754)
                     /locus_tag="OO575_001493"
     CDS             complement(16062..16754)
                     /locus_tag="OO575_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6664507.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            16928..17641
                     /locus_tag="OO575_001494"
     CDS             16928..17641
                     /locus_tag="OO575_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6664508.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            17673..18431
                     /locus_tag="OO575_001495"
     CDS             17673..18431
                     /locus_tag="OO575_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664509.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            18477..19826
                     /locus_tag="OO575_001496"
     CDS             18477..19826
                     /locus_tag="OO575_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6664510.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            19826..20650
                     /locus_tag="OO575_001497"
     CDS             19826..20650
                     /locus_tag="OO575_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664511.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            20662..21222
                     /locus_tag="OO575_001498"
     CDS             20662..21222
                     /locus_tag="OO575_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6664512.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            21253..22323
                     /gene="lptF"
                     /locus_tag="OO575_001499"
     CDS             21253..22323
                     /gene="lptF"
                     /locus_tag="OO575_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6664513.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            22320..23399
                     /gene="lptG"
                     /locus_tag="OO575_001500"
     CDS             22320..23399
                     /gene="lptG"
                     /locus_tag="OO575_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6664514.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(23435..24606)
                     /locus_tag="OO575_001501"
                     /pseudo
     CDS             complement(23435..24606)
                     /locus_tag="OO575_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(24606..24854)
                     /locus_tag="OO575_001502"
     CDS             complement(24606..24854)
                     /locus_tag="OO575_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6664515.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(24887..25801)
                     /locus_tag="OO575_001503"
     CDS             complement(24887..25801)
                     /locus_tag="OO575_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6664516.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(25798..27528)
                     /locus_tag="OO575_001504"
     CDS             complement(25798..27528)
                     /locus_tag="OO575_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR6664517.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            27890..29031
                     /locus_tag="OO575_001505"
                     /pseudo
     CDS             27890..29031
                     /locus_tag="OO575_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(29155..29925)
                     /gene="glcC"
                     /locus_tag="OO575_001506"
     CDS             complement(29155..29925)
                     /gene="glcC"
                     /locus_tag="OO575_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6664518.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            30176..31675
                     /gene="glcD"
                     /locus_tag="OO575_001507"
                     /pseudo
     CDS             30176..31675
                     /gene="glcD"
                     /locus_tag="OO575_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            31675..32727
                     /gene="glcE"
                     /locus_tag="OO575_001508"
     CDS             31675..32727
                     /gene="glcE"
                     /locus_tag="OO575_001508"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6664519.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            32738..33957
                     /gene="glcF"
                     /locus_tag="OO575_001509"
                     /pseudo
     CDS             32738..33957
                     /gene="glcF"
                     /locus_tag="OO575_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            33962..34366
                     /locus_tag="OO575_001510"
     CDS             33962..34366
                     /locus_tag="OO575_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR6664520.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            34388..36559
                     /gene="glcB"
                     /locus_tag="OO575_001511"
     CDS             34388..36559
                     /gene="glcB"
                     /locus_tag="OO575_001511"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6664521.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            36914..38500
                     /locus_tag="OO575_001512"
     CDS             36914..38500
                     /locus_tag="OO575_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR6664522.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         9513 a         9759 c        10626 g         9977 t
ORIGIN      
        1 tgaatcttcg tgcgtttgta ggccggataa ggcgttcacg acgcatccgg cattaggtgc
       61 tcaatgcctg atgcgctacg cttatcaggc ctacaaaatc tattgcaaca tgttgaatct
      121 tcatgcgttt gtaggcggat aaggcgtttt cgccacatca ggtaagagtg aattcacaat
      181 gatgcccggt tgcttttcac aaccgggcat ttttttaacc taaatgctcg ccgccgcaca
      241 cgccgtgcac ttctgcggtg acgtagctcg actcctgact tgccagataa acatatacag
      301 gggccagttc cgccggttgc cccgcacgct tcatcggcgt tttctgacca aactgcggga
      361 tcttatcctg cgtttgtccg ccggaaattt gcagtgccgt ccagataggg cctggcgcga
      421 caatattcac ccgaatacct ttctccgcga cctgttttgc caggccacgg ctgtagttca
      481 gaatcgccgc cttcgtagcc gcatagtcca gtaaatgcgg acttggctgg tatgcctgga
      541 ttgacgaagt ggtgatgaca cttgcacctt tcggtagcag ggggatcgct tcctgggtta
      601 gccagaacag cgcgaaaacg ttaatggcaa aggtcttttg aaactgttcg ctggtgaggt
      661 ctgcaatatc cggaatggca acctgtttcc cggcgaccag cgccataata tccagcccgc
      721 ctaacgcctt gtgcgcttcg tgaaccagcg aacgggcgaa tttctcatcg cttaaatcgc
      781 ctggcagcag aacggctttg cgtccgcatt cttcaatgat ctttttcaca tcctgagcgt
      841 cttcttcttc cacgggaaga taactgatcg ccacgtcagc cccttcacgc gcgtaagcga
      901 tggcggcagc gcgacctatt ccggaatcgc cccctgtcac cagtgcttta cgatctttca
      961 ggcgaccgct accaacatag gttttctcgc cgcaatccgg tatcggtgtc atcttcgcct
     1021 ggatgcctgg cgtcggttgt ttctgtttgg gatattcacc agtgtaatac tgcgtggtcg
     1081 ggtcttttaa atgagacatc gtttttctcc cttcaggttc aacgtccttt aagggtagac
     1141 gctctcgatg cgttgataag ggaaccagga agatccctaa acctcagaat tatgcgacaa
     1201 aggtttaacg tatatgttga tttgctgttg cgtgctgttt actcaattgc gatatactgt
     1261 tgcccgtttt aactacacga caggaatgta tggaacgttt tcttgaaaat gcaatgtatg
     1321 cttctcgctg gctgcttgcc cccgtgtact ttggcctttc gctggcgtta gttgccctgg
     1381 cgctgaagtt cttccaggag attattcacg tactgccgaa tatcttctcg atggcggaat
     1441 cagatttgat cctcgtgttg ctgtcgctgg tggatatgac gctggttggc ggtttgctgg
     1501 tgatggtgat gttttccggt tatgagaatt tcgtctcaca gctggatatc tccgagaaca
     1561 aagagaagct gaagtggctg gggaaaatgg acgcaacgtc gctgaaaaac aaagtagcag
     1621 cgtcgattgt ggcaatttct tccattcact tactgcgcgt ctttatggat gcgaaaaatg
     1681 tcccggataa caaactgatg tggtacgtca ttatccatct gacgtttgtg ctttctgcat
     1741 ttgtgatggg ctatcttgac cgactgactc gtcataatca ctgatctgat tcgggcgcgg
     1801 ttcgcgcgcc cgttattaac aggtcattta tcggacgacg cctgccacag attcagctcg
     1861 ccatcggcga tatgctgatc aatctgcgcc agctcctcgg tgctaaatgt cagattattc
     1921 agcgcctgca cgttctcctc tagttgctcc gcgcggctgg caccaatcaa taccgacgtc
     1981 acgcgatcat ctttcagcaa ccagcttaac gccatttgcg ccattgattg tccacgctgc
     2041 tgtgccattt cattcaataa gcgcaggctg ttgaggttgg cttcggtaag cattttcggc
     2101 gtcagaccac gaactttatt cccttcacga tgcatccgtg aatcttgcgg aatgccgttg
     2161 agatattttc cggtcagcaa tccctgagcc agaggagtaa aggcaataca gcccacgccg
     2221 ttattttgca gggtatccag caggccgctt ttatccaccc agcggttcag taaattgtac
     2281 gaaggttgat gaattaaaag cggaattttc cactcgtgca gcaactcgac cattttttgc
     2341 gtccgctctg gcgagtaaga ggagatcccg acatacagcg ccttaccgct ttgtaccgca
     2401 tgagccagcg cagaggcggt ttcttccatc ggcgtatttt catcgacgcg atgagagtaa
     2461 aagatatcga catactcaag ccccatacgc ttcaggcttt ggtcgaggct ggcgagcagg
     2521 tatttacgtg aaccgccaga gccgtacggg ccgggccaca tatcgtagcc agccttggta
     2581 gagataatca gttcatcgcg ataagcagca aaatcctcgc gcagcaggcg accaaagttc
     2641 tcttctgcgc ttcctggagg cggcccgtaa ttgttggcta aatcaaagtg cgtaatgcct
     2701 aaatcaaacg ctttacgcag gattgcacgc tgtgattcca gcgcgttaac gtgaccgaaa
     2761 ttgtgccata aaccgagcga taatgcgggc aggcgtaaac cactttttcc gcaatagcgg
     2821 tactgcatct gcccgtaacg ttcgggattc gctaaccaga ccatgacctc tcctttccac
     2881 cgttcaattt cgaaacaatg tttctagttt agcgattcgc cagcgcgtat cccgtagtct
     2941 ggctcacaga gtgacgaaaa actgggcaaa aacacgcgct tatgctttgc ttaaaaaaac
     3001 accagttgag gaatgcaacg atgccgcgtt taaccgccaa agatttccca caagagttgt
     3061 tggattacta cgactattac gttcacggga aaatttcgaa acgtgagttc ctcaaccttg
     3121 cggcgaagta tgcggtgggc gggatgacgg cattagcgtt gtttgatttg ctcaagccaa
     3181 attatgcgct ggcgactcag gtagagttta ccgacccgga gattgttgct gagtacatca
     3241 cgtatccttc gccaaatggt cacggcgagg tacggggtta tctggtgaaa cccgcaaaaa
     3301 tgagcggcaa aacgccagcc gtgggggtgg tgcatgagaa tcgtggactg aatccgtata
     3361 tcgaagatgt ggcacggcga gtggcgaagg cggggtatat cgccctggca cctgacggat
     3421 taagttccgt tggaggttat ccgggaaatg atgataaagg tcgtgagctg caacagcagg
     3481 ttgatccaac caaactgatg aatgatttct ttgccgcaat tgagtttatg caacgctatc
     3541 cgcaagcgac aggtaaagtg ggtattaccg gattttgcta tggcggtggc gtatcgaacg
     3601 cggcggctgt cgcgtatccg gaactggcct gcgcggtgcc gttttatggt cgtcaggcac
     3661 ccactgccga tgtggcgaag attgaagcgc ctttactact ccacttcgcg gaactggaca
     3721 cccgaatcaa cgagggctgg cctccttacg aggcggcgtt gaaagacaat aataaggttt
     3781 atgaggctta tatctatccg ggggttaatc acggattcca taatgattcc acgccccgtt
     3841 atgacaaatc tgccgccgat cttgcctggc aaaggacact gaaatggttc gataaatatc
     3901 tctcctgata ggtttatctc ttacgggatt acgtcttaaa caagtatgaa aaaatagcgt
     3961 gcgcaaaagt cgttctttgc ctaaaatatc gttatatatg gcgatatata atgaatgagg
     4021 tgaacgatga ataaccattt tggtaaaggc ttaatggcgg gattaaaagc aacgcatgcc
     4081 gacagtgcgg ttaatgtgac aaaattctgt gccgattata aacgcggttt tgtattaggc
     4141 tactcacaca ggatgtacga aaagaccgga gatcgccagc ttagcgcctg ggaagcgggt
     4201 attctgacgc gccgctatgg actggataaa gagatggtaa tggatttctt tcgtgagaat
     4261 aattcctgtt ctacgttgcg cttttttatg gccggttatc gcctcgaaaa ttgatcaaac
     4321 atacgtatta tcttgcttta attaattaca ctaatgcttc ttcccttcgt tttagcgccc
     4381 cgccgcagta tcatgatatc gataaccata ataaatgtgt ggtaaatggc gcatcgatcg
     4441 cattattgat tttgcgattg aggcaaaata tatgccaggt tctcgcaacg gaataactat
     4501 aaatgactgg agataacacc ctcatccatt ctcacggcat taaccgtcgt gatttcatga
     4561 agctttgtgc agcattagcc gccaccatgg ggttaagtag caaagccgct gcagagatgg
     4621 ccgaatcggt tactaacccg cagcgtccgc cagttatctg gattggcgcg caggagtgca
     4681 ccggttgtac ggaatctctg cttcgtgcaa cgcatccaac ggtagaaaac ctcgtgctgg
     4741 agactatctc tctggaatat cacgaagtgc tttccgccgc cttcggtcat caggtcgaag
     4801 agaacaaaca taacgctctc gagaagtaca aagggcagta tgtgttagtg gtggatggtt
     4861 ccatcccatt aaaagataac ggtatttatt gcatggttgc cggtgagccg attgtggatc
     4921 acatccgcaa agcggcggaa ggctcagcag ccattatcgc tattggttcc tgctccgcgt
     4981 ggggcggtgt tgccgcagct ggagttaacc caactggcgc agtcagcttg caagaagttc
     5041 tgccaggcaa aaccgttatc aatattccgg gctgcccgcc gaacccgcac aacttcctcg
     5101 cgaccgttgc gcacatcatc acttacggca aaccgccgaa actggatgac aaaaaccgtc
     5161 cgaccttcgc ctatggccgt ctgattcacg aacactgcga acgtcgcccg cacttcgatg
     5221 ctggtcgttt tgccaaagag ttcggtgatg aaggccaccg cgaaggctgg tgcttgtacc
     5281 acctcggctg taaagggcca gaaacttacg gcaactgctc aacgctgcaa ttctgcgatg
     5341 ttggcggtgt gtggccggtg gcgattggtc acccgtgcta tggctgtaac gaaaaaggta
     5401 tcggcttcca taaaggcatc catcagcttg ccaacgtcga aaatcagact ccgcgttcac
     5461 agaaaccgga tgttaacgct aaagagggcg gcaacgtctc tgcaggcgct attggtttgc
     5521 tcggcggtgt ggttgggctg gttgccggtg tcagcgtgat ggcggtgcgt gaactgggtc
     5581 gtcagcaaaa gaaagataac gctgactcac ggggagaata accgtgaaca gacgtaattt
     5641 tattaaagca gcctcctgcg gggcattgct gacgggcgcg ctgccgtctg tcagtcatgc
     5701 ggctgctgaa aaccgcccgc caattccggg atcgctgggg atgttgtacg actcgacctt
     5761 gtgcgtaggc tgccaggctt gcgtcaccaa gtgtcaggat atcaacttcc ctgaacgtaa
     5821 cccgcaaggg gaacagacct ggtcgaacaa cgacaaactg tcgccgtata ccaataacat
     5881 cattcaggtg tggaccagcg gcacaggggt caacaaagac caggaggaga acggctacgc
     5941 gtacattaag aaacagtgta tgcactgcgt cgatccgaac tgtgtctctg tgtgcccggt
     6001 ctctgcactg aaaaaagatc cgaaaaccgg cattgtccat tacgacaaag acgtgtgcac
     6061 cggttgccgt tactgcatgg tcgcctgtcc gtacaacgtg ccgaagtacg actacaacaa
     6121 cccgtttggt gcgctgcata agtgcgagct gtgcaaccag aaaggtgtgg aacgtctcga
     6181 taaaggcggt ctgcctggct gcgtagaagt gtgcccggcg ggcgcggtga ttttcggtac
     6241 gcgtgaagag ctgatggcgg aggcgaaaaa acgtctggcg ctgaagcctg gcagcgaata
     6301 ccactatccg cgtcagacgc tgaaatctgg cgacacttac ctgcatacgg tgccgaaata
     6361 ttatccgcat ctgtacggcg agaaagaggg cggcggtact caggttctgg tactgacggg
     6421 tgtgccttat gaaaatctcg acctgccgaa actggacgat ctttctaccg gtgcgcgttc
     6481 cgaaaatatt caacacaccc tgtataaagg catgatgcta ccactggctg tgctggcggg
     6541 cttaaccgtg ctggttcgtc gcaacaccaa aaacgaccat cacgacggag gagacgatca
     6601 tgagtcatga tccacaaccg ctgggcggca aaatcatcag taaaccggtc atgatttttg
     6661 gaccgttaat cgtcatctgt atgctcctga ttgtgaagcg tctggtgttc ggtctgggct
     6721 ctgtctctga cctgaacggc ggcttcccgt ggggcgtgtg gatcgcgttt gacctgctga
     6781 ttggcaccgg ctttgcctgt ggcggctggg cgctggcgtg ggcggtatac gtctttaacc
     6841 gtgggcaata ccatccgctg gtgcgtccgg cgctgttggc gagtctgttt ggttactcac
     6901 tgggtggctt gtcgatcact atcgacgtgg gtcgctactg gaacctgccg tacttctaca
     6961 ttccgggtca cttcaacgtg aactcggtac tgttcgagac ggcggtctgt atgaccatct
     7021 acatcggcgt gatggctctg gagtttgctc cggcactgtt tgaacgtctg ggctggaagg
     7081 tgtcgctaca acgtctgaac aaggtgatgt tcttcatcat cgcgctcggt gcgctgctgc
     7141 cgaccatgca ccagtcttca atggggtcgc tgatgatctc ggcgggctac aaggtgcatc
     7201 cgctctggca gagctatgaa atgttgccgc tgttctcgct gctgacggcg ttcatcatgg
     7261 gcttctcgat tgtcatcttt gaaggttcac tggtgcaggc gggtctgcgt ggcaacggtc
     7321 cggatgaaaa gagcctgttc gtcaagctga ccaacaccat cagtgtgttg ctggcgattt
     7381 tcatcgggct gcgctttggc gagcttatct atcgcgacaa gctgtcgtta gcgtttgccg
     7441 gtgacttcta ctccgtgatg ttctggattg aagtcctgct gatgctcttc ccgctggtcg
     7501 ttctgcgtgt ggcgaagctg cgtaatgatt cccgtatgct gttcctgtca gcactgagcg
     7561 cactgttagg ttgtgcaacc tggcgtctga cctattcgtt ggtggcattc aacccgggcg
     7621 gcggttacgc ctacttcccg acctgggaag aactgttgat ttctattggt tttgtggcta
     7681 ttgagatttg cgcttacatc gtactcattc gtctactgcc gatacttcct cctttaaaac
     7741 aaaacgatca taatcgtcat gaggcgagca aagcatgagc cagagaatta ctattgatcc
     7801 ggtaacccgt attgaggggc atttacgcat cgattgcgaa atcgaaaatg gcgtcgtttc
     7861 gaaagcatgg gcttccggta ccatgtggcg cggcatggaa gagatcgtga aaaaccgcga
     7921 tccgcgcgat gcatggatga ttgtgcaacg tatctgtggc gtatgtacta ccactcacgc
     7981 gctgtcttcc gttcgtgcgg cagaaagtgc gctgaatatc gacgttccgg ttaacgcgca
     8041 atacatccgt aacatcattc tggctgcgca caccacgcat gaccatatcg ttcatttcta
     8101 tcagctttcg gcgctggact gggtggacat cacttctgca ctgcaagctg acccaaccaa
     8161 agcctccgaa atgctgaaag gcgtttcgac ctggcatctg aacagtccgg aagagttcac
     8221 taaagttcag aacaagatca aagatctggt tgccagcggt cagttgggta ttttcgccaa
     8281 tggctactgg ggtcacccgg cgatgaaact gccgccggaa gtgaacctga ttgcggtagc
     8341 gcactacctg caggcgctgg agtgccagcg tgacgctaac cgcgtcgtgg cgctgctggg
     8401 cggtaaaacg ccgcacattc agaacctggc tgtgggtggt gtcgcgaacc caatcaacct
     8461 cgacgggttg ggcgtgttga accttgagcg cctgatgtac atcaagtctt tcatcgacaa
     8521 actgagcgac tttgttgagc aggtttataa ggttgatacc gcggttatcg ccgcgttcta
     8581 cccggaatgg ctgacgcgcg gtaaaggtgc ggtgaactac ctgagcgtgc cggaattccc
     8641 gaccgacagc aaaaacggca gcttcctgtt cccgggaggc tacattgaga atgcggatct
     8701 gtcctcgtat cgtccgatca cttctcattc cgatgaatac ctgatcaaag gcattcagga
     8761 aagcgcgaag cactcctggt ataaagacga agcgccacag gcgccgtggg aaggtaccac
     8821 cattccggct tatgatggtt ggtctgacga cgggaaatat tcctgggtga aatcaccgac
     8881 tttctacggc aaaacggtag aagtgggtcc gctggctaat atgctggtga aactggcggc
     8941 aggtcgcgaa tctacccaga acaaactgaa tgaaatcgtt gcgatttatc agaaactgac
     9001 tggcaacacg ctggaagtgg cacagctgca ctccacgctg ggccgtatta ttggtcgtac
     9061 cgttcactgc tgtgaattgc aggatatcct gcaaaaccaa tacagtgcac tgatcaccaa
     9121 tatcggcaaa ggcgatcaca ccacctttgt gaagccgaac attccggcaa cgggtgagtt
     9181 caaaggtgtt ggcttcctcg aagcgccgcg cggtatgctc tctcactgga tggttattaa
     9241 agacggtatc atcagcaact accaggcggt tgttccatca acctggaact ctggtccgcg
     9301 taacttcaat gatgacgtcg gtccttacga gcagtcgctg gtgggtacac cggttgccga
     9361 tccgaataaa ccgctggaag tggtgcgtac cattcactcc ttcgacccgt gcatggcctg
     9421 tgcggtacac gtagtggatg ccgacggcaa cgaagtggtt tcagtgaagg ttctgtaatg
     9481 cgtattttag tcttaggggt cggcaatatt ttgctgaccg atgaagccat cggcgtgcgg
     9541 attgtcgaag cgttagagca acgatacatt ctgccggatt atgttgagat cctcgatggc
     9601 ggcacggcgg gaatggagct gcttggcgac atggcaaatc gcgatcatct gattattgcg
     9661 gatgccattg tgtcgaaaaa gaacactccg ggaacgatga tgatcctgcg ggatgaagaa
     9721 gttccggcgt tgtttaccaa caaaatctct ccgcatcagc ttggcctggc cgacgtattg
     9781 tcggccctgc gcttcaccgg tgagtttccg aaaaagctga ccctggtcgg cgtgatcccg
     9841 gaatcgctgg agccacacat cggcttaacg ccgacggttg aagcaatgat tgaacctgcg
     9901 cttgagcagg ttctggctgc gctgcgcgaa tcaggcgtgg aagccatccc acgggaggcg
     9961 attcatgact gaagagatag caggtttcca gacctccccg aaggcgcaag tacaggcagc
    10021 gtttgaagaa attgcccggc gttcgatgca cgatctctct tttctgcatc cttcaatgcc
    10081 ggtgtatgtt tctgatttta cgctgttcga aggtcagtgg acggggtgtg tgatcacccc
    10141 gtggatgctg agtgcagtta tcttccccgg cccggatcaa ctctggccgc tgcgcaaagt
    10201 gagtgaaaaa attggtctgc aactgccgta tggcaccatg acctttaccg ttggcgaact
    10261 ggacggtgtt tcgcaatacc tctcctgttc gctgatgtcg ccgctttcgc acagcatgtc
    10321 gattgaagaa ggccaacgcc tgacggatga ctgcgcacga atgatccttt cgttgccagt
    10381 caccaatccg gatgtaccac acgcagggcg tcgcgccctg ctgtttggcc gcaggagggg
    10441 cgaaaatgca tgagttgtcg ctttgccaga gcgccgttga aattatccaa cggcaggcgg
    10501 agcagcacga tgttaagcgc gtcaccgccg tgtggctgga aattggcgcg ctctcctgcg
    10561 ttgaggagag cgccgtccgt tttagttttg aaattgtctg ccacggaacg gtggcgcaag
    10621 ggtgcgattt acatatcgtc tataaacccg cccaggcctg gtgctgggat tgcagccagg
    10681 tggtggagat tcatcagcac gatgcgcagt gtccgctctg tcacggcgag cggttgcgtg
    10741 tcgataccgg cgattcgctg atcgtcaaaa gtattgaagt tgaataagac tgaggacaac
    10801 gcctaaaaag cgcacggacg gtcccctcgc ccctccgggg agagggttag ggtgagaggg
    10861 aacacccgct aaaggttctt tttcgcgttt gtccggcaat cgaaataacc ggagttaaca
    10921 atgtgtattg gcgttccagg ccaggtgctg gctgtcggtg aagatattca ccagcttgcg
    10981 caggttgaag tatgtggtat caagcgcgac gtgaatatcg ccctgatttg cgaaggtaac
    11041 cctgccgatc tgctgggcca gtgggtgctg gtacacgtcg gatttgccat gagcatcatc
    11101 gacgaagatg aagccaaagc cacattagac gcactgcgcc aaatggatta cgacattacc
    11161 agcgcgtgat gattagcttt tgtaggcctg ataagacgcg gcagcgtcgc atcaggcatt
    11221 gtgcacgatt gccggatgcg gcgtgaacgc cttatccggc ctggatggct tgctgcgacg
    11281 aacaccaacc cttacccctg acgcttatct tctgtatttg tctcaaaatc actggcgtca
    11341 tggcgctcat gcaactgctc gttcagcggc ccgttggtgc ggttaacaat acgcccacgt
    11401 ttcactgccg gacgttcgcc cacttctttc gcccagcgtt gcacatgttt ataacttccc
    11461 gcatccagaa actctgcggc atcatacacg ccgccgagca ccacgttgcc aaaccacggc
    11521 caaatcgcca tatccgcaat ggtgtactca tcgcccgcaa caaacttatg ctgcgccagt
    11581 tgcttatcca gcacgtcgag caggcgcttg gcttccatgg taaagcggtt gatggcgtac
    11641 tcaattttca ccggcgcgta atggtaaaag tgaccaaaac caccgccgag gaacggcgcg
    11701 gcaccctgta accagaacag ccagttcaac gtttcagtcc gttttgccag atcctgcggc
    11761 aggaagtagc caaatttctc cgccagataa agcaggatcg aaccagattc aaacacgcgg
    11821 atcggcggat tatgcgtatg atcgcgcagc gccgggatct tcgagtttgg gttcacttcg
    11881 acaaagccgc tggagaattg atcgccatcg ccaatacgaa tcagccaggc gtcgtactct
    11941 gcaccagtaa cgcccagcgc cagtagctcc tcaagcataa tcgttacttt ctgaccgttc
    12001 ggcgttccca gcgaataaag ttgcaatgga tgtttgccaa cgggcagcgt tttttcatgc
    12061 gtcggaccag aaaacgggcg attgatattg gcgaacgcgc cgccagcgga tttatcccac
    12121 gtccagactt tcgcgggctg ataagtattg tctgtcatag tgagttgcct tctgagtggt
    12181 agtgttgaag cagtgtagca ggtcacttgc tgtacagaat gtgaattttt taaccctttg
    12241 cgccagccgc tgcgatgagg tgtatgatta gtctgacctc tgtccggtta atgtcgctga
    12301 gcaggtaatc agagcacaac aattacgcca actgcttagg ttgtattgtt aaaggtaaag
    12361 tgatgagcaa aggaacgacc agccaggatg ccccgttcgg gacattattg ggctacgccc
    12421 caggtggggt agcaatctac tcttcagatt acagttctct cgatccgcag gaatacgaag
    12481 atgacgccgt attccgtagc tatatcgacg acgaatatat gggccacaag tggcaatgcg
    12541 ttgaatttgc tcgccgtttt ctctttctga attacggtgt ggtctttact gacgtgggta
    12601 tggcgtggga gattttctcg ctgcgcttcc tgcgagaagt ggttaatgac aacatcctgc
    12661 cattgcaggc atttcctaac ggctcgccgc gtgcgccggt cgcgggtgcg cttcttatct
    12721 gggataaagg cggtgaattt aaagacactg gccatgtcgc catcattacc caattgcatg
    12781 gcaacaaagt ccgtattgcg gaacagaacg tgattcattc cccgttgccg caagggcaac
    12841 agtggacgcg cgagctggag atggtggtcg aaaacggctg ctataccctt aaagacactt
    12901 ttgatgacac caccattctg ggctggatga tccagacgga agatactgaa tacagcttac
    12961 cgcagccgga aattgcaggc gagctgctga aaatcagtgg agcgcgtctg gaaaacaaag
    13021 gccagtttga cggtaaatgg ctggatgaaa aagatccgct acaaaacgcc tatgtgcagg
    13081 ccaacggtca ggtgatcaat caggatccgt atcattacta caccattacc gagagtgccg
    13141 agcaggagct aattaaagcc accaacgagc tgcatctgat gtatcttcac gcaaccgaca
    13201 aggtgctgaa agatgacaac ctgctggcgc tgttcgacat tccgaaaatc ctctggccac
    13261 gtttgcgtct ctcctggcag cgtcgccgtc accatatgat cactggtcgt atggatttct
    13321 gcatggatga gcgtggcctg aaggtttacg aatacaacgc cgactccgcc tcctgtcata
    13381 ccgaagcggg cttgatcctc gaacgttggg cggagcaggg ctataaaggc aacggcttca
    13441 atccggcgga agggctgatt aacgaactgg ctggtgcctg gaaacacagt cgtgcacgtc
    13501 cgtttgtcca tatcatgcag gacaaagata tcgaggaaaa ctatcacgcg cagtttatgg
    13561 agcaggcgct gcaccaggcg ggctttgaaa cgcgtatctt gcgcgggctg gatgaactgg
    13621 gctgggatgc tgccgggcaa ctgattgatg gggaagggcg actggttaac tgcgtgtgga
    13681 aaacctgggc gtgggaaacc gcgtttgatc agattcgtga agttagcgac cgtgagtttg
    13741 ctgcggtgcc aatccgtacc ggtcatccgc aaaacgaagt gcgtcttatc gacgtattgc
    13801 tgcgcccgga agtgctggtc tttgagccgc tgtggacggt gatccccggc aacaaagcga
    13861 ttctgccgat cctctggtcg ctgttcccgc accatcgtta cctgctggat accgatttca
    13921 ctgttaatga tgaattggtg aaaacaggtt acgcagtgaa accgatcgcc ggtcgctgtg
    13981 gtagcaatat cgacctggtc agccatcatg aagaggtgct ggacaaaacc agcggtaaat
    14041 ttgccgagca gaaaaacatc tatcagcaac tgtggtgttt gccgaaagtg gacggtaaat
    14101 acattcaggt atgtaccttc accgttggcg gcaactacgg tgggacgtgt ttgcgcggtg
    14161 atgaatcact ggtcatcaaa aaagagagtg atattgaacc gttaattgtg gtgaaaaagt
    14221 aatacatgtt gtagcaaccc cgtactgatt atgaagaata attagtacgg ggattaatca
    14281 aaatattcaa tatttatata ttccaatatt tattcatttc atatgtgaat atattaacca
    14341 gtggaatacc tgtgttttga ttatttctaa aggttttgaa tgaatgctta ttgtctgata
    14401 cacaagaaat aacactcttt ttatcgttaa aaaatgatat ttcactttgc ccatgccgtt
    14461 aaagtcactg ataatgcgtc cgttcgtaaa ttcaaaatgg cgtaatctaa tatatgctaa
    14521 atttatttgt tggccttgat atatatacgg ggcttttgtt attacttgct ctggcatttg
    14581 tgttgttcta cgaagcaatc aatggttttc atgacacggc gaatgcggtg gcaaccgtta
    14641 tttatactcg tgccatgcag ccacaacttg ctgtggtgat ggcggcattt tttaactttt
    14701 ttggcgtgtt attgggcgga cttagcgttg cctatgccat tgtccatatg ttaccaatcg
    14761 atttgttgct gaatatgggg tcaactcacg gtttggcgat ggtcttttcc atgctgctgg
    14821 cggcgattat ctggaacctg ggaacgtggt tctttggttt accggcctcc agttcgcaca
    14881 ccttgattgg tgcgattatc ggcatcggtt taaccaacgc gctgttaacc ggctcatcgg
    14941 tgatggatgc gttaaacctg cgtgaagtaa ccaaaatttt ctcctcgctg attgtttccc
    15001 ctatcgtcgg cctggtcatt gcgggtggtc tgattttcct gctgcgccgt ttctggagtg
    15061 ggacgaaaaa gcgtgaccgt attcaccgca ttccggaaga tcgcaaaaag aaaaagggca
    15121 aacgtaaacc gccattctgg acgcgtattg cgctgattgt ttccgctgcg ggcgtggcgt
    15181 tttcgcacgg cgcgaacgac gggcagaaag ggatcggcct ggtaatgctg gtactggtgg
    15241 ggattgcccc tgctggcttc gtcgtcaata tgaatgcgtc cggctatgaa attacccgta
    15301 cccgcgatgc cgttaccaac ttcgaacact acctgcaaca gcatcctgaa ctgccgcaga
    15361 agttgattgc gatggaacct ccattgcctg cagcatcgac tgatggcacg caagtaacag
    15421 agtttcactg tcatccggca aatacctttg atgcgattgc gcgcgttaaa acgatgctgc
    15481 caggcaatat ggaaagttac gagccgttaa gcgtgagtca gcgcagccag ctgcgccgca
    15541 ttatgctgtg catctccgat acctccgcga agctggcgaa actgccaggc gtcagtaaag
    15601 aagaccagaa cctgctgaaa aaactgcgca gcgatatgtt aagcaccatt gagtacgctc
    15661 cggtgtggat catcatggcg gtagcactgg cgctcggcat tggcaccatg attggctggc
    15721 gtcgtgtagc gatgaccatc ggtgagaaga ttggtaagcg cggcatgacg tatgcgcaag
    15781 gcatggcggc acaaatgacg gcggcagtgt ctatcggcct tgccagttat attgggatgc
    15841 ctgtctccac aacacacgtc ctctcgtctg cggtagcagg gacgatggtg gtggacggcg
    15901 gtgggttaca gcgtaaaacg gtaaccagca tcctgatggc gtgggtgttt actttaccgg
    15961 cggcaatttt tctttctggt gggctgtact ggatagcatt gcagttgatt taaccttcct
    16021 gaaaatgccc ggtcctggcg atcgggcatt tccatttttg actagtgata accacgcgcg
    16081 gtcataaaat ccgtaatcgc tttttctgca tcaaccaaca cctgttccag tggctgattg
    16141 gcatcgatat caaccagttg tgcaccacca aaggttaact gtggcgttat ggcaatcttc
    16201 ctcgccagcg attcccgttt atggtcgggt ttacgtgcac aggcaacttc aaggtcaaca
    16261 ttgagtttga tgaccagatc aggcttatgg ctcgccatcc agtgaaacgc tttacgttcc
    16321 tggcttgcca gccatgagac aaaacgacca ccttcaacgt taggtgggaa caccgtacca
    16381 tcgtaagcgc caggaatttg gtcctgagga taacggtcgg ttagaacaat taacccgcga
    16441 cgacgacagg caagcatatg acgaaagcgc agtaagcgac gggcgacaaa cgctgtaatt
    16501 accagcgccg gaactggtcc aggcaatttt tttgctgttt tcacctgatt tcgttcaatt
    16561 gttttatgta aggattttcc catcaaaggt aattttgtca ctgcacgacc gacatttccg
    16621 gcctgttttc ctaaatgaac tctttcggca gcaccatatt tttcgacaac ggtaataaga
    16681 tgttcacaca ccgttgactt gcctgaagca tcgctaccaa taacggcaat taatggaggt
    16741 gttattgact gcatgaatat atcctttaat ttaaatatcc attaaaaata tttatttggt
    16801 taatatgttt ttatgaaagc gtaattcagg tcaatgtcac aattagtcat tgtcacaata
    16861 aggttgaacg gatattctga gcacggacca cgtaatattc aatacattat ttactgccgg
    16921 gtttttcgtg aatcaacgta atatgtcaat aattaattcc acgccagtgc gtgttattgc
    16981 cattgtagga tgtgatggtt caggtaaatc gaccctcacg gcaagcctgg taaatgaact
    17041 ggcagcaaga atgccaacag aacacattta tctcgggcaa tcgtccgggc gaattggcga
    17101 atggatttca cagctccctg ttattggcgc accttgtggg tgttatctgc gaagtaaagc
    17161 ggcacatgta cacgaaaagc ccttaactcc gcctggcaat attactgcgc tggttatcta
    17221 tctgctttcc tgctggcggg cgtacaagtt tcgcaaaatg ttgtgtaaaa gccagcaagg
    17281 ctatctgctc atcaccgacc gctacccgca ggttgaagtg ccgggttttc gctttgatgg
    17341 cccgcaattg gcaaaaacca cgggcggtaa cggttggata aaaatgttaa ggcagcgcga
    17401 gttgaagctg taccaatgga tggcgtctta tttacccgta ttgttgattc gtcttggcat
    17461 tgatgaacaa accgcgtttg cgcgtaaacc tgaccaccaa ctggcagcgt tacaggaaaa
    17521 aatcgccgtt acgccgcaac tgacattcaa tggcgccaag atccttgaac tggatggtcg
    17581 gcaaccagcc gatgaaatta tgcaagcgtc actacgcgca attcacgccg cgctttcctg
    17641 atcaccaaaa tggatagggg atgatttaac gaatggatgc actacaaaca caaactgtta
    17701 atagtacaac cgcgccgcag cccaactaca ttccggggct gattgcggtg gtcgggtgtg
    17761 atggcaccgg taaatccacg ctgaccaccg acctggtgaa atcgctgcaa caacactggc
    17821 aaaccgagcg ccgctatctg gggctgctct ccggcgaaga cggcgacaaa atcaaacgat
    17881 tgccgttggt tggcgtctgg ctggaacggc gattggcggc caaatcctcg aaaacccaaa
    17941 gcatgaaaac caaatctccg gcgctatggg cggcggtgat tatgtactgc ttctcgctgc
    18001 gaagaatggc gaatctacgc aaggttcagc gactggcgca aagtggcgtt ctggtggtca
    18061 gcgatcgctt cccgcaggct gaaatttcgg gcttttatta tgacggaccg gggatcggcg
    18121 tcgaacgtgc gaccgggaaa atcagcatgt ttctggcgca gcgcgaacgg agtttatacc
    18181 aaaaaatggc gcaataccgt cctgaattaa tcattcgttt ggggattgat attgatactg
    18241 ccatctcccg caagcctgac catgagtatg ccgagctgca ggacaaaatc ggcgtcatgt
    18301 cgacgattgg ctataacggc acaaaaattc ttgagattga ttccagagcg ccctacagcg
    18361 aagtgctgga acaggtacag aaggcggttt ctctggttgc catcgtttct gaccgccgaa
    18421 gtttaactta aggctggcgg gatatttttt actatcaacg ataaggtcag gctgaattgg
    18481 cgggttttaa catcaaacat tggtttgcag atggcgcgtt tcgcaccatt attcgcaata
    18541 gcgcctggtt aggctccagt aatgtcgaga gcgccttgct ggggctgttg gcgctctcgt
    18601 gtgccggtaa agggatgacg cccgccatgt ttggtgtact ggtgattgtg caatcgtacg
    18661 ccaagtcgat cagcgatttt attaagtttc agacatggca gctggtggtt cagtacggaa
    18721 cgccagcctt gaccaacaat aatccgcagc aatttcgcaa tgtcgtctca ttttccttct
    18781 cgctggatat cgtcagcggc gcggtggcga ttgtcggcgg cattgcctta ctgccattcc
    18841 tttcccattc attaggtctg gatgaccaaa gcttttggct ggcggcgctg tattgcacgc
    18901 tcattccttc aatggcttcc tccacgccga ccggcattct gcgtgcggta gatcgctttg
    18961 atttgattgc tgtacagcag gcgacgaaac cttttctgcg cgcagcgggg agcgtcgcag
    19021 cctggtattt tgactttggt tttgtgggtt ttgttattgc ctggtacgtg tcgaatctgg
    19081 ttggcggcac catgtactgg tggtttgccg cgcgcgaatt acgccgtaga aatatccata
    19141 acgccttcaa attgaatctg tttgagtctg cccgacacat taaaggcgcg tggagttttg
    19201 tctggtcaac caacattgcc cactccatct ggtcggcgcg taactcgtgc agcacagtgt
    19261 tggtggggat cgtgttagga cccgctgccg ccgggttatt taaaatcgcc atgacattct
    19321 tcgacgccgc cggaacgcca gcgggtttgc tgggtaaaag tttttaccca gaggtgatgc
    19381 gtttagatcc gcgcaccacc agaccgtggt tgttaggtgt gaagtccggt ttactggcgg
    19441 gcggaatcgg tattctggtg gcgctcgcgg tgttgatcgt cggcaagccg ctcatttcgc
    19501 tggtgtttgg cgttaagtat ctcgaagcgt atgacctgat tcaggtgatg ctgggcgcaa
    19561 ttgtgatttc gatgctgggc ttcccgcagg agtcattgct attgatggcg ggaaaacagc
    19621 gcgcttttct cgtggcgcaa accatcgcct caatcggcta catcgtattg ctgttcatgt
    19681 tctgtcatct gtttggtgtg ctgggagcag cgtttgctta cttcggtggg caatgtctgg
    19741 acgtggcgct ctcgttaatt ccaaccttaa aggcattttt tcagcgccat tccttgcttt
    19801 ataacgcagc cggagagaaa tcctgatgat gagaaaatat ttcccgcttg aggcgagcga
    19861 gcgtctattt gttgcgattg aagaagatga tgtcgttgat gcacaggtta gtttgccgcc
    19921 gactattgca ctaagttgta ccaccgaaat cattcatgat aattacgcgt tatgcctgca
    19981 attctggctc aatggcgttg atcgtcagga attactgcgt ttagttcgca aacaagccaa
    20041 aggcgatgag ctgactgctg atgagcgcaa acaatttaaa tacatgcgag cgcgctacaa
    20101 gcacctgcgt tttgcgcaac ggctgtattt aaagaagcat caggccggat ttttattcgg
    20161 caaaacgacc gtttttctgg ggcgttttca ggacggcttt cgtaacggta agaaaaatat
    20221 tgtgtcgtat tacggcaacc tgttacggat ttatttaagc tctccggtct ggtcgttggt
    20281 gaactattca ttacgccata gccagttaga aagcgtaagc agttttattg cctatcggca
    20341 aaagcaaatg cataccctga aagagattat tgccaaaccg cgactaaccg gcagggaatt
    20401 tcatgatgtg cgaaaaatca tcagccagca ggtttcttat tacgacacct taagatcgct
    20461 tgatcctgaa aataaagagg cgttacagat ttcccgtttc ctcgccgcca ttaatggcct
    20521 gatgggagat aaacacgacg acatggtggc ggatgatatg gaaaaccgcc agtcgtatta
    20581 tgcaccgttg gcgctggata gcgatatttg tcagcgctta gagctgctga tttcgcgttt
    20641 tccgctatag ccggagcaat gatgtcatct cgtttgatta ttgcgttgat tatcatgctc
    20701 ttagccccgg gtgtgcaggc acacaatttc gtcactggca aaacggttac ccctgtttat
    20761 atccaggaag gcggcgagct gcttctgaat agcgacgacg aaattcacta ccaaaagtgg
    20821 aacagcacac aattagcggg aaaagtccgc attattcagt atatcgccgg gcgtaagtcg
    20881 gcgaagaaaa agaactcact gttaattaaa gcggttgaag cggcaaactt tccgcaggat
    20941 cgttttcagc ccaccaccat tgtgaacacc gatgatgcca tctttggtac tggctatttt
    21001 gttgttggca aaatcgaaaa aaataaacgc cgctatccgt gggcgcaatt cgttattgac
    21061 ggcaatggac agggacgcgt tgcctggcgc ttgccggagc agagttcaac cattctggtg
    21121 ctgaataaag ccgggcaaat tcagtgggca aaggacggca gcctgacgcc ggaagaggta
    21181 gatcatgtca ttgcgttggc gcagaaactg atcaatgaat aaaaaactca aaggattgag
    21241 ggcgttagtg agatgaagct ggttgaacac tacatcatgc gtggtacgcg ccgtctggtg
    21301 ctgattatcg tcggttttct gatctttatt ttcgccagct actccgcaca gcgttatctg
    21361 accgaagcgg caaatgggac attagctctt gatgttgtgc tggatatcgt tttctacaaa
    21421 gtactgattg cactggagat gttgttacct gttggtctgt atgtgtcagt tggcgtgacg
    21481 ctagggcaga tgtataccga ctcggaaatt accgctatct ctgcggcggg gggcagtccg
    21541 ggacgcttgt acaaagccgt tctttatctg gcgataccgc taagtatttt tgtcaccctt
    21601 ctgtcgatgt atggtcgtcc gtgggcttat gcgcagattt atcaactgga gcagcagtcg
    21661 cagtcggagc tggatgttcg ccagttgcgg gcaaagaaat ttaacactaa cgataacgga
    21721 cgaatgatcc tttcgcagac ggttgatcag gataataatc gcctgactga cgcgctgatt
    21781 tatacttcta ctgccaatcg aacccgtatt ttccgcgccg gttcggttga tgtggttgac
    21841 ccatcacccg agaaaccgac cgttatgttg cacaacggca ctgcctatct tctcgatcat
    21901 caggggcgtg acgacaacga acagatctac cgtaatctgc aattacatct gaatccgctg
    21961 gatcaaagcc cgaacgtcaa acgcaaagcg aaatcggtca cggagctggc gcgctccgcc
    22021 tttcctgccg atcatgccga actgcaatgg cgacaaagcc gtggcctgac cgcattgttg
    22081 atggcgctgc tggccatttc attaagtcgg gtaaaaccgc ggcaagggcg attttcaacg
    22141 ttattgccac tgacgttgct gtttattgcc attttttatg gcggcgacgt ctgccgtaca
    22201 ctggtggcta acggcgcgat tcccctcatt cctggtttgt ggttagtgcc cggactcatg
    22261 ctaatgggcc tgctgatact ggtcgcacgt gacttctctt tgctgcagaa attttcccga
    22321 tgaatgtttt cagtcgctat ttaatccgtc atctctttct cggttttgcc gccgctgcag
    22381 ggctattgct gccgcttttt accaccttca acctgattaa cgaactggat ggtgtcagcc
    22441 cgggcggtta tcgctggact caggcggtgc tggtggtgct aatgacctta ccgcgcacac
    22501 tggtcgaact ttcgccgttt atcgcgttat tgggtggtat tgttggactg gggcagttat
    22561 cgaaaaacag tgagcttacc gccattcgca gcacggggtt ttctatcttc cgtattgcac
    22621 tggtggcgct ggttgccggg atattgtgga ctgtttcgtt aggcgcgata gatgagtggg
    22681 tggcgtcgcc attgcagcaa caggcgttgc aaatcaaatc gaccgccacc gcgttggggg
    22741 aggacgatga cattaccggc aatatgctgt gggccaggcg tggtaatgaa tttgtgacgg
    22801 tgaaatcgct gaacgagcag ggccagccgg tgggcgtgga gatatttcat tatcgcgacg
    22861 atctctcgct tgaatcctac atttatgcac gcagtgccac cattgaagac gacaaaacgt
    22921 gggtcctgca tggtgtgaat cataaaaaat ggctgaatgg caaagaaacg ctggaaacat
    22981 tagataatct tgcctggcaa tcggccttca ccagtatgaa tcttgaagag ttatcaatgc
    23041 cggggaatac tttttctgtc cgtcagctta atcattacat ccattatttg caggaaaccg
    23101 ggcaacccag cagcgaatac caccttgcac tgtgggaaaa actggggcaa cccatcctga
    23161 ccctggcgat gattttgctg gctgtgccgt ttacctttag tgccccgcgt tcgcctggga
    23221 tgggtagccg tctcgctgta ggcgtcatcg tgggcttact cacctggatc agctatcaaa
    23281 tcatggttaa tctgggattg ctatttgcgt taagcgcacc tgttaccgcg ctcggtttac
    23341 cggtagcgtt tgtgttggtg gcgttgagcc tggtgtattg gtatgacaga caacattaaa
    23401 cccgccgtgg cgcaaacacc acggcgggct cacattactg cttcagagta gcaaatgcct
    23461 gaatgatggc atcgatttgc tcacgactgt gggcagcatt tacgctacag cgcagcagcg
    23521 tgatgcctgc aggcgcggca ggcggcagga taagattcac atacacgccc ggcgagataa
    23581 gctcgcgcca gatgcgtaaa ccttcctctt tcgaaccgat tattactggc actaccgggc
    23641 taatatgtga acttaactca taacccagtt tttgcagacc gtcatacaga tggttggcgt
    23701 tatccatcag cttttgccgc agctctggtt gtgtggcaat agttcttaac gacgagcgca
    23761 cggtcgcaat gcaggacgga gaaggagagg cggtaaaaat atacgggcgg ctgccgtagc
    23821 gcagcacttc catcgcttcc gaaacgacag caaaaccgcc aatcgaggcc agacttttac
    23881 tgaaagtacc aacgataata tccacatcat cttcaacgcc gacagcttca gccagtccgc
    23941 gccccgttgc gcctaacacg ccaaacgaat gcgcttcatc gacaatcaaa taaccaccga
    24001 gacgacgttt gatatcaaca atttccgcca gcggcgcaac gtcaccgagc atgctgtaga
    24061 tgccctcaac gatgataatc gcctctttcg cccgttcacc gaggcgaacc atgcgccgtt
    24121 ccagatcttt agcatcgtta tggcggaaac gaataatttc cgcgccaccg agagaacaga
    24181 cgtcataaat actggcgtgg ctgtcggcat ccagtaacac cacggcgtta tggtcggcaa
    24241 gtgcgctgat gacgcctaaa ttagcggtat aaccggttga aaaacgatag cggtagggcg
    24301 attgaaaaac tcggcaatct ctttctctaa cgccaggtgc ggcgcataac tgccgtttgc
    24361 catacgagaa ccggtggtgc cggtgccttg ggccgctaac gcggcctgac cttcgctgat
    24421 ggcatcatga ttaaaggtta aaccgagata attattggtc ccggccagaa tcactttttt
    24481 attgccgatc cggccttcgg tggcggaata cacttcatca atacaggtgc caaacggtgt
    24541 taacccgttg tcactaaatt gcagacgctc gccagcaagg cgcgcatatt tatcgtatag
    24601 ccccattatt tattctccag ccatggaagt agagtttcca ttaattgcgc aggggttttg
    24661 acatccagca gaatattaat aggaatggaa atatcgaatc tgtcttccag catcattaag
    24721 agatccatca ctttgatcga ctccaggcca agatcgttga cgagatcgct gtcgggctta
    24781 atttccacac cgttttcgac caaatcctgt aaacaggaaa ggatgtaatc cattactatt
    24841 tcacgattta ccatagaaac gttaacacac ctttaaaaca agacaatcaa aatagcccgt
    24901 tgcgtaatgc gtgttccagg ctaatcccgg gaaaccaatt gatatcttca gaaatacggt
    24961 tattacttgc cgaccagtcg gcgtgggtta attcacgaat tttcgagcgg gtcagcatag
    25021 gttctttacc tgccaggcga ctcaacgcgg tactgatatc cgcaaggcct gtgagtaccg
    25081 gcagaggaat gtcaaccatt cgcacggaac cacaacgggc tttggcggca agctgtcgta
    25141 cgcgttgcca gtcatagcca ccggcgacgc catcgcataa ttcataggtt tgcgtctgta
    25201 cagtttcggc gcttaaccac tgacccactg cttgcgcaaa atcggtgacg tgaagaaaag
    25261 agagttgtgt atctggcgca ccaagtcgtg gcagcaggcc gcgtagcatc cagtcaaaca
    25321 gcggttttaa ctctttatcg ccgggaccat acacggctgt cgggcgaaaa acgcccagcg
    25381 taatttcgtc cgccattgct gccagtcgtt gctcggcgac gtatttggat tttgcatacc
    25441 aggagagctc gggatggcgc gccgccagcg aagagataaa cagaaaacgt tggcaaaagc
    25501 cgctctcttt tgctgcttgc atcaggcgca ggctgccgtc aacgttacag cgggtgaaaa
    25561 tctcttcttt gtgcccgcgc acttgcccgg cgcaatggac cactacgctg gctccggcaa
    25621 ccagctcgct aagtgaatga gtatcttcca gcgaaccgcg cacccaggtg agattatcat
    25681 tgacgtgagc gcgggcagta cgcgtcaatg cgcgaacgtg aaagccgcgg gcgagcaggt
    25741 tatcgataat atatttaccg ataaacccgg tagcgcccgt caccgcgaca gtttgattta
    25801 tgccagggat tcctgcacat taagactggc agcataagcc ttctgataac gttttttcgc
    25861 ttccgcacgg gcaggcttgc cggaggacgt tcggggaata ctgtggggcg gcaacagatc
    25921 gatagccgcg gtcacgccaa attcgctttg gatccgtgcc gccagcgcgt ggataagctg
    25981 cccgcgacgt tcttcgtcgc tgatccgaca ctggatctgc aaaatgattt tttcctgggc
    26041 ggtaacaaaa gcaatcgcat cgccagaatg aatttccggt tcctgttccg ctatatattc
    26101 aatatcctgc ggccagatat tacggccacg aataataatc agatctttaa tgcgtccggt
    26161 gacatacaga taaccgtcca gcagataacc gaggtcgccg gtgtctaacc agcccgtcgc
    26221 ggcaatctcg tcttgcgaaa cctggtcgcc aaagtaaccg ctcatcagac tgggaccgga
    26281 gatgcaaata tggcctacca cacgttccgc gaccggcata cctgcttcat tgcggatttc
    26341 aataccatgt tccggcaacg ctttgccgca gttgacgaaa gtcgatacgg cgcgtgtctc
    26401 tgcacccggc gcgacggctt taccctgata ttcgaggatg tcgcgatcca cttcgttaac
    26461 cacaaccccg gaggcttcat cagagaagct gacagccagc gcattttctg ccagtccgta
    26521 gcacggcatg aaagttttat tgtcaaagtt aacctgacgg aaacattcag cgaattgatg
    26581 gagttgttct gcggagatcg gctcagcacc aataccagcg acgcgccagc aggaaagatc
    26641 cagttcagcg agatcttttt cattcacgcg gcgttggcac aattcatagc caaacggcgg
    26701 cgcaacggaa acggtgccgc gatttttact gatcaattta agccattgca aaggacgcat
    26761 ggcaaaatcc tgagtgcgca aataatctac tgaaagttgc gtggcgacgg ggggcaggag
    26821 aaagccgacc agtcccatat catggtagaa aggctgccag gagacgcagc ggtcgctagg
    26881 gcgtaattta atgccatcgt ggcttatagc acgtagatta gccatcactt cgcgatgggt
    26941 gataatgacg ccacggggaa aacgggtgct gccggaggtg tactggaggt aggcgatatc
    27001 gttcggaact ggacgctgga gcgcaacatc ggcttccggt aatgccttaa accaagcgtg
    27061 gcttaaaaca tgtaattcgg ggttgttatg cgtcgcggca ttgaccagtg gcaaccactc
    27121 atcaccagtg ataatggctg cgggctggca acttgccagt aaaccctgga gtttggcgct
    27181 ccaggaatcc cgctgaccga cgcccattgg aatcgccaac gggacggcga ctaagccagc
    27241 atactgacag gcaaaaaaag cctctacaaa cccgctactc gtttcggcaa tcagcgccac
    27301 gcgttcgcct tttttcaggt tcagcgataa caaccgcttc gcaccagctt cggcacgcgt
    27361 ttttaatgtc tgatattcca gttgatcttc aagttggcaa cgtctgtcat aaaaattcat
    27421 tccggctcta ctcagagcgg cgtagtccaa agcatcaacc agcgttggaa aatcggcata
    27481 gcgcataggt agggaatgcg taaagatttt attagacata tacaccacga aaaaattgga
    27541 tatcaaaaat aaaatgacaa taatgcggtg gattatatag gcataaaaga taaatgagat
    27601 agtattttca tcggtgtgaa aatgaacatt gatttaaata atgagtttaa gttttttgtt
    27661 tttattatga atgatattta atttatgaat atattaagaa gaagccttgt ccggtcgcct
    27721 taaattgaca aaaggcattg aggcgtttct gtgtagcctc tgaccttatc ataatcatta
    27781 attaatacta tttattcgtc gtttgtattc attgagttga ttattaagat gccgctcgtt
    27841 gctgttgcaa cctgtttttc accattaatt atttgttatc cacagagata tggaatgcga
    27901 tctgctgatg attaaaattg aaaaagtaat taataaaaat gaccttaagg catttatcgc
    27961 ttttccgtca tcactttatc ccgacgatcc aaactggata ccccctttat tcattgaacg
    28021 caacgagcat ttgtctgcga aaaatccggg gacggaccat atcatctggc aggcgtgggt
    28081 ggcaaaaaaa gcggggcaga tagttgggcg tattaccgcg caaatagata ccttgcaccg
    28141 tgagcgttac ggtgaagata ccggtcattt cggcatgatt gacgccattg atgatccgca
    28201 ggattttgcc gcgttgtttg gcgcagcgga agcgtggttg aagtcacaag gtgcaagtaa
    28261 gatcagcggg cctttcagcc tgaatatcaa tcaggaaagc ggattactga ttgaaggttt
    28321 tgacacacca ccctgtgcga tgatgccaca cggcaaaccg tggtatgccg cgcatattga
    28381 acaactgggt tatcacaaag gcattgattt actggcgtgg tggatgcagc gaaccgatct
    28441 cactttctct ccggcgctaa aaaaactgat ggatcaggtg cgcaaaaagg tgaccattcg
    28501 ctgcatcaat cgtcagcggt ttgccgaaga gatgcagatc ctgcgtgaga ttttcaactc
    28561 tggctggcag cacaactggg gatttgtgcc gtttaccgaa catgaattcg cgaccatggg
    28621 cgaccaactt aaatatctgg tgccggacga tatgatctac atcgctgaga ttgattctgc
    28681 accctgcgcg tttattgtgg cttgcctaac atcaacgagg cgattgccga tctgaacgga
    28741 tcgctctttc ccttcggctg ggcaaaattg ctgtggcgtt tgaaagtcag cggtgtgcga
    28801 actgcgcgag taccgctgat gggcgtgcgc gacgagtatc agttcagccg catcggcccg
    28861 gtgattgcgc tgttattgat tgaagcctta cgcgatccgt ttgcccgccg gaagatcgat
    28921 gcgctggaga tgtcatggat cctcgaaacc aatacaggca tgaataacat gctggaacgc
    28981 attggggcgg agccgtacaa gcgttaccga ttgtatgaaa agcaaattta ataccagatc
    29041 ctgtttagcg caggggcggt cattcgggag agggtataga tgaacagatt taacaccaga
    29101 tcctgttaag tgcaggggcg gtcccctcgc cttggggata gggtaaaact ctgctcacat
    29161 tcccaccttg ttcatctcca gcggcaccgc cgagcgaatc aaatggtggc cttccagctc
    29221 gatatcgtgg agattctttt tcacggtccg tacatgatcg cgcgctgcgc gctgagcgac
    29281 gtgcggcagc cgctgcaaca ccgcgttgta gatccgcgca tgctggcgat cgatctgctt
    29341 tttttgctgt ggtcgatggt agagattatt tacgctggca aacactgaat taaacatcag
    29401 atcggtcaat gattgcagcg taaacaccag cacctgattg tgagaagcct gacaaatggc
    29461 gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc gaaatctctt tgtggttctc
    29521 actggcggcg agcatttttt cataacagcg ggttatcaca acaaaatcag cctgcgttcc
    29581 cagcgttgcc gccagccttg ccgattcgcc ctccagtaat gcgcgaacgt cgagcagatc
    29641 gtacagcgtt cgcggctgcg tactgaacag atggatcagc gggctggtgt cctgctcccg
    29701 attaagccgt gcgacatgag aatcgcgacc ctgcgccgtt tcaataatcc cgcgcccgcg
    29761 cagcacggtc agcccttcac gcagtgcgga gcgtgagaag ccgagctttt cacacagtcg
    29821 acgttccgag ggaagcggct gaccgacctt cagtacgccg tcgataatta accgttcgat
    29881 actctctgca accacttcgc aaatagggcg acgttcatct ttcattcccg gacctcgtgc
    29941 acaggtagga ccaatttttc tgttcttaac tcgcaaaaca cgcacatcac gcaagtgatt
    30001 atgttacatc aatttaacat taagttaacc aagacaaggt cacagagctg gaaaaaaaat
    30061 ggtctgaccg gtagctaaag agatagacga aaacgaaaag cccgcttaat aactgttcac
    30121 agaagcagcg cgcaaaaatc agctgccaca caacacaaca aagcgaagcc tactcatgag
    30181 catcttgtac gaagagcgtc ttgatggcgc tttacccgat gtcgaccgca catcggtact
    30241 gatggcactg cgtgagcatg tccctggact tgagatcctg cataccgatg aggagatcat
    30301 tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt ccattactgg ttgttctgcc
    30361 taagcaaatg gaacaggtga cagcgattct ggctgtctgc catcgcctgc gtgtaccggt
    30421 ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg ctgccgctgg aaaaaggtgt
    30481 gttgttggtg atggcgcgct ttaaagagag cctcgacatt aaccccgttg gtcgccgcgc
    30541 gcgcgtgcag ccgggcgtat gtaacctggc gatctcctag gccgttgcgc cgcataatct
    30601 ctattacgcg ccggatcctt cctcacaaat cgcctgttca attggcggca atgtggcgga
    30661 aaatgccggt ggcgtccact gcctgaaata tggtctgacc gtacataacc tgctgaaaat
    30721 tgaagtgcaa acgctggacg gcgaggcgct gacgctggga tcggacgcgc tggattcacc
    30781 tggttttgac ctgctggcgc tgttcaccgg atcggaaggt atgctcggcg tgaccaccga
    30841 agtgacggta aaactgctgc cgaagccgcc cgtggcgcgg gttctgttag ccagctttga
    30901 ctcggtagaa aaagccggac ttgcggttgg tgacatcatc gccaatggca ttatccccgg
    30961 cgggctggag atgatggata acctgtcgat ccgcgcggcg gaagatttta ttcatgccgg
    31021 ttatcccgtc gacgccgaag cgattttgtt atgcgaactg gacggcgtgg agtctgacgt
    31081 acaggaagac tgcgagcggg ttaactacat cttgttgaaa gcgggcgcga ctgacgtccg
    31141 tttggcacag gacgaagcag agcgcgtacg tttctgggcc ggtcgcaaaa atgcgttccc
    31201 ggcggtagga cgtatctccc cggattacta ctgcatggat ggcaccatcc cgcgtcgcgc
    31261 cctgcctggc gtactggaag gcattgcccg tttatcgcag caatatgatt tacgtgttgc
    31321 caacgtcttt catgccggag atggcaacat gcacccgtta atccttttcg atgccaacga
    31381 acctggtgaa tttgcccgcg cggaagagct gggcgggaag atcctcgaac tctgcgttga
    31441 agttggcggc agcatcagtg gggaacatgg catcgggcga gaaaaaatca atcaaatgtg
    31501 cgcccagttc aacagcgatg aaatcacgac cttccatgcg gtcaaggcgg cgtttgaccc
    31561 cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta caccgctgtg ctgaatttgg
    31621 tgccatgcat gtgcatcacg gtcatttacc ttttcctgaa ctggagcgct tttgatgcta
    31681 cgcgagtgtg attacagtca ggcgctgctg gagcaggtga atcaggcaat tagcgataaa
    31741 acgccgctgg tgattcaggg cagcaatagc aaagcctttt taggtcgccc agtcaccggg
    31801 caaacgctgg atgtgcgttg tcatcgcggc attgttaatt acgacccgac cgagctggtg
    31861 ataaccgctc gtgccggaac gccgctggtg gcgattgaag cggcgctgga gagcgccggg
    31921 caaatgcttc cctgcgagcc gccgcattat ggtgaagaag ccacctgggg cgggatggtc
    31981 gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt cggtccgcga ttttgtcctc
    32041 ggcacgcgca tcattaccgg cactggaaaa catctgcgtt ttggtggcga agtgatgaaa
    32101 aacgttgccg gatacgatct ctcacggtta atggccggaa gctacggttg tcttggcgtg
    32161 ctcactgaaa tctcaatgaa agtgttaccg cgaccgcgtg cctctctgag cctgcgtcgg
    32221 gaaatcagcc tgcaagaagc catgagtgaa atcgccgagt ggcaactcca gccattaccc
    32281 attagtggct tatgttactt cgacaatgcg ttgtggatcc gccttgaggg cggcgaagga
    32341 tcggtaaaag cagcgcgtga actgctgggt ggcgaagagg ttgtgggtca gttctggcag
    32401 caattgcgtg aacaacaact gccgttcttc tcgttaccag gtaccttatg gcgcatttca
    32461 ttacccagcg atgcgccgat gatggattta cccggcgagc aactgatcga ctggggcggg
    32521 gcgttacgct ggctgaaatc gacagccgat gacaatcaaa tccatcgcat cgcccgcaac
    32581 gctggcggtc atgcgacccg ctttagtgcc ggagatggtg gctttgcccc gctatcggct
    32641 cctttattcc gctatcacca gcagcttaaa cagcagctcg acccttgcgg cgtgtttaac
    32701 cccggtcgca tgtacgcgga actttgagga gcaggctatg caaacccgat taaccgaaga
    32761 gatgcggcag aacgcgcgct ggaagccgac agcatcctgt gcgcctgtgt tcactgcgga
    32821 ttttgtaccg caacctgccc aacctatcag cttctgggcg atgaactgga cgggccgcgc
    32881 gggcgcatct atctgattaa acaggtgctg gaaggcaacg aagtcacgct taaaacacag
    32941 gagcatctcg atcgctgcct cacttgccgc aattgtgaaa ccacctgtcc ttctggtgtg
    33001 cgctatcaca atttgctgga tatcgggcgt gatattgtcg agcagaaagt gaaacgccca
    33061 ctgccggagc gaatgctgcg cgaaggattg cgccaggtag tgccgcgtcc ggcggtcttc
    33121 cgtgcgctga cgcaggtagg gctggtgctg cgaccgtttt taccggaaca ggtcagagca
    33181 aaactgcctg ctgaaacggt gaaagctaaa ccgcgtccgc cgctgcgcca taagcgtcgg
    33241 gttttaatgc tggaaggctg cgcccagcct acgctttcgc ccaacaccaa cgcggcaact
    33301 gcgcgagtgc tggatcgtct ggggatcagc gtcatgtcag ctaacgaagc aggctgttgt
    33361 ggcgcggtgg actatcatct taatgcgcag gagaaagggt tggcacgggc gcgcaataat
    33421 attgatgcct ggtggcccgc gattgaagca ggtgccgagg caattttgca aaccgccagc
    33481 ggttgcggcg cgttcgtcaa agagtatggg cagatgctga aaaacgatgc gttatatgcc
    33541 gataaagcgc gtcaggtcag tgaactggcg gtcgatttag tcgaacttct gcgcgaggaa
    33601 ccgctggaaa aactggcaat tcgcggcgat aaaaaactgg ccttccactg cccgtgtacc
    33661 ctacaacatg cgcaaaagct gaacggcgaa gtggaaaaag tgttacttcg tcttggattt
    33721 accttaacgg acgttcccga cagccatctg tgctgcggtt cagcgggaac atatgcgtta
    33781 acgcatcccg atctggcacg ccagctgcgg gataacaaaa tgaatgcgct ggaaagcggc
    33841 aaaccggaaa tgatcgtcac cgccaacatt ggttgccaga cgcatctggc gagcgccggt
    33901 cgtacctctg tgcgtcactg gattgaaatt gtagaacaag cccttgaaaa ggaataaaaa
    33961 aatgaaaact aaagtcattc ttagccagca aatggcgagt gcaattattg ccgcaggtca
    34021 ggaagaggcg cagaaaaata actggtctgt ttccattgct gttgccgatg acggcggtca
    34081 tctgctggcg ttaagtcgca tggacgattg cgcgccgatt gcggcttaca tctcccagga
    34141 gaaagcgcgt accgccgcgc tggggcgtcg tgaaactaag ggctatgaag agatggtgaa
    34201 caacggacgt accgcgttcg tgactgcgcc gttactgacg tcgctggaag gcggcgtacc
    34261 ggttgttgtt gatgggcaaa ttattggtgc cgtgggcgtt tctggtttaa ccggagcaca
    34321 ggatgcgcag gtcgcgaaag tggcagcagc ggtgttggcg aaataagcga aaacgaggag
    34381 agaaacaatg agtcaaacca taacccaggg ccgtttacgc attgacgcca attttaaacg
    34441 ttttgtggat ggagaagttt tgccgggcgt ggaactggac gctgctgcat tctggcacaa
    34501 tgttgatgag atcgttcacg atctggcacc agaaaatcgt cagttgctgg cagaacgcga
    34561 tcgcattcag gcggcgcttg atgagtggca tcgcagcaat ccggggccgg taaaagataa
    34621 agcggcctat aaatctttcc ttcgtgaact gggctacctg gtgccgcaac cggagcgcgt
    34681 gacggtggaa accacgggca ttgacagcga aatcaccagt caggcggggc cacagctggt
    34741 ggttccggca atgaacgccc gctacgcgct gaacgcggcg aacgctcgct ggggctcact
    34801 gtacgatgcg ttatacggca gcgacatcat cccgcaggaa ggggcgatgg tcagtggcta
    34861 cgatccgcag cgcggtgagc aggttatcgc ctgggttcga cgtttcctcg atgaatcact
    34921 accgctggaa aacggcagct atcaggatgt ggtggcgttt aaggtggtcg ataaacaatt
    34981 acgcatccag ttgaaaaatg gtaaagaaac cacgttacgt actccagcac agtttgtcgg
    35041 ttaccgtggc gatgccgctg cgccgacctg cattttgctg aaaaataacg gcctgcatat
    35101 tgaactgcaa attgatgcca acgggcggat tggcaaagac gatccggcgc acatcaacga
    35161 tgttatcgtc gaagctgcta tcagtaccat tctcgactgc gaagattcgg tcgcggcggt
    35221 tgatgcggaa gataaaatcc tgctgtaccg caatctgctg ggcctgatgc aggggactct
    35281 gcaagagaaa atggagaaaa acggtcggca aatcgtacgt aaactgaatg acgatcgtca
    35341 gtacaccgcc gccgatggct ctgaaatttc tctgcacgga cgctcgttgc tgtttatccg
    35401 caacgtgggt catttgatga ccattcctgt gatttgggac agcgaaggca atgaaatccc
    35461 ggaaggtatt cttgatggcg tcatgactgg cgcgattgcc ctctatgatt taaaagtgca
    35521 gaaaaactcg cgcactggca gcgtctatat tgtgaaaccg aaaatgcacg gtccgcagga
    35581 agtggcgttc gccaacaaac tgtttacccg cattgagaca atgctcggta tggcaccgaa
    35641 taccctgaaa atgggcatta tggatgaaga acgccggacc tcgctgaact tgcgtagctg
    35701 tatcgctcag gcgcgcaacc gcgtggcgtt catcaatacc ggtttcctcg accgtaccgg
    35761 cgatgaaatg cattcggtga tggaagctgg cccgatgctg cgtaaaaacc agatgaaatc
    35821 gacgccgtgg atcaaagcct acgaacgtaa taacgtgctt tccggtctgt tctgtgggct
    35881 gcgcggtaaa gcgcaaatcg gtaaaggcat gtgggcaatg ccggacctga tggcagacat
    35941 gtacagccag aagggcgacc aactgcgtgc cggggcaaac acagcctggg ttccgtcacc
    36001 aaccgctgct acgctccatg cgctgcacta ccaccatacc aacgtacaga gcgtgcaagc
    36061 caacattgcc cagaccgagt tcagtgctga atttgaaccg ctgctggacg atctgctgac
    36121 tattccggtg gctgaaaacg ctaactggtc ggcgcaagag atccaacaag agctggataa
    36181 caacgtgcag gggattctgg ggtacgtggt gcgttgggtg gagcagggga ttggttgttc
    36241 aaaagtgccg gatattcaca acgtggcgct gatggaagac cgcgcaacgc tgcgtatctc
    36301 cagccagcat atcgccaact ggttacgtca cggtattctg accaaagaac aggtgcaggc
    36361 gtcgctggag aatatggcga aagtggttga tcagcaaaat gctggcgatc cggcttatcg
    36421 tccgatggcg gggaatttcg ctaactcgtg tgcttttaaa gctgccagcg atttaatctt
    36481 cctcggcgtg aaacagccaa acggttatac cgaaccgtta ttacacgcct ggcgtttacg
    36541 cgaaaaagaa agtcattaat agcgtcaaaa aagccccggc aagttttatt gtcggggcta
    36601 tgatagtaac tgcggtagta catttatccc ggaaataacc ggagcgatta tcagggttta
    36661 acaatattaa atacccgtag cgcattacta gcgggcctaa aacaacaata tggacatctt
    36721 aacgatactc tgcgcctgcg tgtgcggaac agaaaccctt acccgatata cttgttatgc
    36781 cagaggtagc aattgcgtta gcggcgtact gttgctgtaa agtataacgc ctatatttta
    36841 cagttcagtc ctcttttgct tattacaacc ttaagtatat caagcatata aagataataa
    36901 gagactgaac aatatggtta cctggaccca aatgtatatg ccgatgggag gactggggct
    36961 atccgctctg gtcgccctga tcccgataat attcttcttc gttgcactcg cggtattacg
    37021 tctgaaagga catgttgctg gagcaataac ccttatatta tctatcctga ttgcgatatt
    37081 cgcctttaaa atgccgattg atatggcatt tgctgctgcg ggctatggct ttatttatgg
    37141 attatggcca attgcgtgga ttattgtcgc ggcggtgttc ctgtataaat taaccgttgc
    37201 cagcgggcag ttcgatatta tccgcagctc ggttatctcc atcaccgacg atcagcgttt
    37261 gcaggtgtta ctgattggtt tctcctttgg tgcgttgctg gaaggagctg ctggctttgg
    37321 tgcgacggtg gcgtttggtg cgttgggcgt gccgattctg gtcgccggtc aggtaacggg
    37381 aatcgatccg ttccacattg gcgcaatggc gggacgtcag ttaccgttcc tgtcggttct
    37441 tgtgccgttc tggctggtag caatgatgga cggctggaaa ggggtgaaag agacgtggcc
    37501 agcggcgctg gttgctgggg gaagcttcgc tgtcactcag ttctttacct ctaactatat
    37561 tggtccggaa ctgccggata ttacttcggc gctggtgagt atcgtctcac tcgctttatt
    37621 ccttaaagtc tggcggccga aaaataccga aacggcaatc agcatggggc aatccgcagg
    37681 tgcgatggtg gtaaataagc catcttctgg cggtcccgtg ccttcagaat atagtctggg
    37741 gcaaatcatt cgtgcgtggt caccgttttt aatcttaacg gtgctggtca ccatctggac
    37801 catgaagccg tttaaagcgt tatttgctcc gggcggcgcg ttttattcac tggtgattaa
    37861 tttccagatc cctcatttgc atcaacaagt gttgaaagcg gcacccattg tcgcccaacc
    37921 aacgccaatg gatgcggtgt ttaaattcga ccctctctcg gctggcggca ccgctatttt
    37981 tattgcggcg attatctcta tcttcatcct cggcgtgggc atcaagaaag ggattggcgt
    38041 ctttgccgaa atgctaatta gtctgaagtg gccgatattg tcgattggca tggtgctggc
    38101 attcgccttc gtcaccaact attccggcat gtccaccacg ctggcgctgg tactggcagg
    38161 tacaggcgtg atgttcccgt ttttctcacc gtttctcggc tggctgggcg tattccttac
    38221 cgggtcggac acttcttcaa acgccctgtt tggttcactg caatcgacca cggcgcagca
    38281 aatcaacgtc tctgacactc tgctggtggc agcaaatacc agcggcggcg taactggcaa
    38341 gatgatctcc ccgcaatcta tcgccgtggc ctgcgccgcg acgggcatgg tgggccgaga
    38401 atctgaactg ttccgctaca ccgtgaagca cagtctgatt tttgccagcg ttatcaccct
    38461 gctgcaggcg tatgtgttta ccgggatgtt agtctcgtaa atcaataaag ccggatgaag
    38521 gtaaatatca cccggctttc catttgtctg tcgtttatct cgccagggaa ccgtttttac
    38581 gattggcttc tttatacgta gtctccgttt tacgatgcga attgtttaat atttatacaa
    38641 cttggcttct gtgctggtta taaagtaccc actttcatga gatcaatttt agtatgttat
    38701 tacattgctt catctgttgc tttcatgacg aatggcgttt tttctcttat ttatattttt
    38761 cactagaaca atgagtagat caaaattccc aaaactccca aaaacataag attattcgca
    38821 aagctgattt tgcagaataa tgagaacttt taagatttgt cgaggatcaa ttttaagcaa
    38881 tttatctcca cttgttacat aaaagatgcc gttaggttaa taagttaatc ttgctataac
    38941 agtgttcgtt actgcctatt aataaagata tttgggatag ggtaacgtgg atgaggattc
    39001 gaaatcatcc gtcttgcgga aagtgaaatg ttggcagatg ttaatattat gtatgtattt
    39061 taactccaac aacacaccaa tcattttgtt agaaataaga aaatttatgg ttaaataatt
    39121 tggtgattat gaagcattaa atcgtaatgc caaatattgt tcgctttgga gagtattatt
    39181 gcctgtttgt gtctgttgag cagaaaaatc atctgaattt gaatactttt attaggtgtg
    39241 aataaatgaa aaaatcatta aaatcattaa aatcattaaa atcattaaaa tcattaaaat
    39301 cattaaaatc attaaaatca ttaaaatcat taaaatcatt aaaatcatta acttatatga
    39361 atatttcaga gttggagttc gaatgttgac acctgagtat ttcctgctaa aaatagccct
    39421 gctttacgga tggttgtatt gcaataacac gaaagtagta agagattaaa ggtatctgtc
    39481 agcgataact ctgcaagtga tgacattttg taaaccaaat aatgtaaatc agcaatgtat
    39541 cttttcactt aattataaca aatgtggtat tcactaagaa tctgatacga gcttacatca
    39601 cgactatttt agtctcatcg cgggatacag ggatgcaccg atggatctca gcacaccgta
    39661 atgcaatacc tgaacaaata gaacagtaaa gtggttcagt attaatgaaa gctcttattt
    39721 tccgttggga aaacttaaaa tagatgagaa ggctttttga tttcttatgt gatctcattt
    39781 cgagtattga gaaatttgtg atttttaaat ttttcaataa gccctgtaag ggattggggt
    39841 tttatgagaa ataaagagac gtattgtctt tgttt
//
LOCUS       DAJTYM010000029        38304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTYM010000029 DAJTYM010000000
VERSION     DAJTYM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..107)
                     /locus_tag="OO575_001513"
     CDS             complement(<1..107)
                     /locus_tag="OO575_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HCR6664523.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEA"
     gene            390..431
                     /gene="hisL"
                     /locus_tag="OO575_001514"
     CDS             390..431
                     /gene="hisL"
                     /locus_tag="OO575_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6664524.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    407..530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            577..1476
                     /gene="hisG"
                     /locus_tag="OO575_001515"
     CDS             577..1476
                     /gene="hisG"
                     /locus_tag="OO575_001515"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6664525.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1482..2786
                     /gene="hisD"
                     /locus_tag="OO575_001516"
     CDS             1482..2786
                     /gene="hisD"
                     /locus_tag="OO575_001516"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6664526.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2783..3853
                     /gene="hisC"
                     /locus_tag="OO575_001517"
     CDS             2783..3853
                     /gene="hisC"
                     /locus_tag="OO575_001517"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6664527.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3853..4920
                     /gene="hisB"
                     /locus_tag="OO575_001518"
     CDS             3853..4920
                     /gene="hisB"
                     /locus_tag="OO575_001518"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6664528.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4920..5510
                     /gene="hisH"
                     /locus_tag="OO575_001519"
     CDS             4920..5510
                     /gene="hisH"
                     /locus_tag="OO575_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6664529.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5510..6247
                     /gene="hisA"
                     /locus_tag="OO575_001520"
     CDS             5510..6247
                     /gene="hisA"
                     /locus_tag="OO575_001520"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6664530.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6229..7005
                     /gene="hisF"
                     /locus_tag="OO575_001521"
     CDS             6229..7005
                     /gene="hisF"
                     /locus_tag="OO575_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6664531.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6999..7610
                     /locus_tag="OO575_001522"
     CDS             6999..7610
                     /locus_tag="OO575_001522"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6664532.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7796..8773)
                     /gene="wzzB"
                     /locus_tag="OO575_001523"
     CDS             complement(7796..8773)
                     /gene="wzzB"
                     /locus_tag="OO575_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6664533.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8919..10039)
                     /gene="ugd"
                     /locus_tag="OO575_001524"
                     /pseudo
     CDS             complement(8919..10039)
                     /gene="ugd"
                     /locus_tag="OO575_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10289..11695)
                     /gene="gndA"
                     /locus_tag="OO575_001525"
     CDS             complement(10289..11695)
                     /gene="gndA"
                     /locus_tag="OO575_001525"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6664534.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11804..12163)
                     /locus_tag="OO575_001526"
     CDS             complement(11804..12163)
                     /locus_tag="OO575_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664535.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12160..12666)
                     /locus_tag="OO575_001527"
     CDS             complement(12160..12666)
                     /locus_tag="OO575_001527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664536.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12812..13375)
                     /locus_tag="OO575_001528"
     CDS             complement(12812..13375)
                     /locus_tag="OO575_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664537.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13582..14520)
                     /locus_tag="OO575_001529"
                     /pseudo
     CDS             complement(13582..14520)
                     /locus_tag="OO575_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14583..14957)
                     /locus_tag="OO575_001530"
     CDS             complement(14583..14957)
                     /locus_tag="OO575_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR6664538.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14961..16109)
                     /gene="wzy"
                     /locus_tag="OO575_001531"
     CDS             complement(14961..16109)
                     /gene="wzy"
                     /locus_tag="OO575_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6664539.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16129..17034)
                     /gene="rfbG"
                     /locus_tag="OO575_001532"
     CDS             complement(16129..17034)
                     /gene="rfbG"
                     /locus_tag="OO575_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6664540.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17027..17917)
                     /locus_tag="OO575_001533"
     CDS             complement(17027..17917)
                     /locus_tag="OO575_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6664541.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17947..19203)
                     /gene="wzx"
                     /locus_tag="OO575_001534"
     CDS             complement(17947..19203)
                     /gene="wzx"
                     /locus_tag="OO575_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6664542.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19219..19764)
                     /gene="rfbC"
                     /locus_tag="OO575_001535"
     CDS             complement(19219..19764)
                     /gene="rfbC"
                     /locus_tag="OO575_001535"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6664543.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19769..20647)
                     /gene="rfbA"
                     /locus_tag="OO575_001536"
     CDS             complement(19769..20647)
                     /gene="rfbA"
                     /locus_tag="OO575_001536"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6664544.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20706..21605)
                     /gene="rfbD"
                     /locus_tag="OO575_001537"
     CDS             complement(20706..21605)
                     /gene="rfbD"
                     /locus_tag="OO575_001537"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6664545.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21605..22690)
                     /gene="rfbB"
                     /locus_tag="OO575_001538"
     CDS             complement(21605..22690)
                     /gene="rfbB"
                     /locus_tag="OO575_001538"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6664546.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23063..23956)
                     /gene="galF"
                     /locus_tag="OO575_001539"
     CDS             complement(23063..23956)
                     /gene="galF"
                     /locus_tag="OO575_001539"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6664547.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24131..25525)
                     /gene="wcaM"
                     /locus_tag="OO575_001540"
     CDS             complement(24131..25525)
                     /gene="wcaM"
                     /locus_tag="OO575_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6664548.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFVPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25536..26756)
                     /gene="wcaL"
                     /locus_tag="OO575_001541"
     CDS             complement(25536..26756)
                     /gene="wcaL"
                     /locus_tag="OO575_001541"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6664549.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26753..28033)
                     /gene="wcaK"
                     /locus_tag="OO575_001542"
     CDS             complement(26753..28033)
                     /gene="wcaK"
                     /locus_tag="OO575_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6664550.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28213..29691)
                     /gene="wzxC"
                     /locus_tag="OO575_001543"
     CDS             complement(28213..29691)
                     /gene="wzxC"
                     /locus_tag="OO575_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6664551.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29693..31087)
                     /gene="wcaJ"
                     /locus_tag="OO575_001544"
     CDS             complement(29693..31087)
                     /gene="wcaJ"
                     /locus_tag="OO575_001544"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6664552.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31142..32512)
                     /gene="cpsG"
                     /locus_tag="OO575_001545"
                     /gene_synonym="manB"
     CDS             complement(31142..32512)
                     /gene="cpsG"
                     /locus_tag="OO575_001545"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6664553.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32705..34141)
                     /gene="cpsB"
                     /locus_tag="OO575_001546"
     CDS             complement(32705..34141)
                     /gene="cpsB"
                     /locus_tag="OO575_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6664554.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34144..35367)
                     /gene="wcaI"
                     /locus_tag="OO575_001547"
     CDS             complement(34144..35367)
                     /gene="wcaI"
                     /locus_tag="OO575_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6664555.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35364..35843)
                     /gene="gmm"
                     /locus_tag="OO575_001548"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35364..35843)
                     /gene="gmm"
                     /locus_tag="OO575_001548"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6664556.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35843..36811)
                     /gene="fcl"
                     /locus_tag="OO575_001549"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35843..36811)
                     /gene="fcl"
                     /locus_tag="OO575_001549"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6664557.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36814..37935)
                     /gene="gmd"
                     /locus_tag="OO575_001550"
     CDS             complement(36814..37935)
                     /gene="gmd"
                     /locus_tag="OO575_001550"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6664558.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(37961..>38304)
                     /gene="wcaF"
                     /locus_tag="OO575_001551"
     CDS             complement(37961..>38304)
                     /gene="wcaF"
                     /locus_tag="OO575_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6664559.1"
                     /translation="WKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQKSYLCTGSHDH
                     ASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPANVVCRGNP
                     AVVIRERVETE"
BASE COUNT        10234 a         9598 c         8630 g         9842 t
ORIGIN      
        1 gcctctccat tctgacgagt aatgaggatc ggggcatgat cttcaacggc tttcatcatt
       61 gttgccgaca aattctgacg cgcttcgctg tagctaattg tacgcatgta aatctcctat
      121 tttgtacagt tcattgtaca atgataggtg ttaattaact atttattaat tagtttgtag
      181 atcaagacat tgccagcgag acgaaaaatc agactttgct ctttttggtg ctgtcaagtt
      241 agaggacagt cctcttagcc ccctcctttc cccgctcatt cattaaacaa atccatcgcc
      301 ataaaatata taaaaaagcc cttgctttct aacgtgaaag tggtttaggt taaaagacat
      361 cagttgaata aacattcaca gagattttta tgacacgcgt tcaatttaaa caccaccatc
      421 atcctgacta gtctttcagg cgatgtgtgc tggaagacat tcagatcttc cagtggtgca
      481 tgaacgcatg agaaagcccc cggaagatca ccttccgggg gcttttttat tgcgcggttg
      541 ttaacggttc agacaggttt aaagaggaat aagaaaatga cagacaacac tcgtttacgc
      601 atagctatgc agaaatccgg ccgtttaagt gatgactcac gcgaattact ggcgcgctgt
      661 ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga tggcagaaaa catgccgatt
      721 gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg taatggacgg cgtggtagac
      781 cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg cgcccagggt
      841 gaagatccgc gctactttac cctgcgtcgt ctggatttcg gcggctgccg cctttcgctg
      901 gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct taaacggtaa acgtatcgcc
      961 acctcttatc ctcacctgct caagcgttat ctcgaccaga aaggtatctc ttttaaatcc
     1021 tgcttactga acggttctgt cgaagttgcg ccgcgtgccg ggctggcgga tgcgatttgc
     1081 gatctggttt ccaccggtgc cacgctggaa gctaacggcc tgcgcgaagt cgaagttatc
     1141 taccgctcga aagcctgcct gatccagcgc gatggcgaaa tggaagaatc taaacagcaa
     1201 ctgatcgaca agctactgac ccgtattcag ggtgtgatcc aggcgcgcga atcaaaatac
     1261 atcatgatgc acgcaccgac cgaacgcctg gatgaagtca tcgccctgct gccaggtgcc
     1321 gaacgcccaa ccattctgcc gctggcgggt gatcaacagc gcgtagcgat gcacatggtg
     1381 agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga aagcactggg tgccagttca
     1441 atcctggtcc tgccgattga gaagatgatg gagtgatcgc catgagcttt aacacaatca
     1501 ttgactggaa tagctgtact gcggagcaac aacgccagct gttaatgcgc ccggcgatct
     1561 ccgcttctga aagcattacc cgcaccgtta acgatattct cgataacgtg aaaacgcgtg
     1621 gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa aaccacggtt accgcgctga
     1681 aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct gagcgacgag ctaaaacagg
     1741 cgatggcggt ggcagtaaag agtattgaaa ccttccacac tgcgcaaaaa ctgccgccgg
     1801 tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt cacgcgcccg gtggcttcag
     1861 tgggtttgta tattccgggc ggctccgccc cgctcttctc aacggtatta atgctggcaa
     1921 ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg ctcaccgccg ccgattgccg
     1981 atgaaattct ttatgcggcg cagctgtgcg atgtgcagga cgtgtttaac gtcggcggcg
     2041 cacaggccat tgccgcgctg gcgtttggta cggaatctgt gccgaaagtg gacaaaatct
     2101 tcgggccggg taacgccttt gtcaccgaag caaaacgcca ggtaagccag cgtctggacg
     2161 gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct ggtgattgct gacagcggcg
     2221 ctacgccgga ttttgtggct tctgatttgc tttctcaggc tgaacacggc cctgactcac
     2281 aggtaatttt actgaccccc gatgctgata tggcgcgtcg cgttgccgag gccgtcgaac
     2341 gccaactggc agaactgccg cgtgccgaaa ccacccgcca ggcactgaac gccagccgcc
     2401 tgatcgtgac taaagattta gcgcagtgcg tggagatctc caaccagtac ggcccggagc
     2461 acctgatcat tcagacccgc aacgcccgcg aactggtcga tggcatcacc agcgccggtt
     2521 cggtatttct tggtgactgg tcaccggaat cggcaggcga ctacgcctcc ggcaccaacc
     2581 acgtgctgcc gacttacggt tacaccgcca cctgttccag cctcgggctg gcagatttcc
     2641 agaaacgcat gaccgtacag gaactgtcga aagaaggttt ctccgcgctg gcttcgacca
     2701 ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa aaatgccgtt actttgcgtg
     2761 ttaacgtcct taaggagcaa gcatgagcac cgtgactatt actgatttag cgcgtgaaaa
     2821 cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg ggcggtaacg gcgacgtctg
     2881 gctgaacgcc aacgaatatc ccacagccgt ggagtttaag cttactcagc aaacgctcaa
     2941 ccgctacccg gaatgccagc caaaagcggt gattgaaaat tacgcgcaat atgcaggcgt
     3001 aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa ggtattgaac tgctgatccg
     3061 cgctttttgc gaaccgggta aagacgccat cctctactgc ccgccaacgt acggcatgta
     3121 cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca gtgccgacgc tggaaaactg
     3181 gcaactggac ttacagggca tttccgacaa gctggacggc gtaaaagtgg tctatgtttg
     3241 cagccccaac aaccccaccg ggcaactgat caatccgcag gattttcgca ccctgctgga
     3301 gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct tatatcgagt tttgcccgca
     3361 ggcatcgtta gctggctggc tggcggaata tccgcacctg gcgattttgc gtaccctttc
     3421 gaaagccttc gcgttggcgg ggcttcgttg cggatttacg ctggcaaacg aagaagtcat
     3481 caacctgctg atgaaagtga tcccccccta cccgctctcg acgccggttg ccgacattgc
     3541 cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc gaacgggtgg cgcaaattat
     3601 tacagaacgc gaatacctga ttgccgcact gaaagagatc ccctgcgtgg agcaggtttt
     3661 cgactctgaa accaactaca ttctggcgcg ctttaaagcc tccagtgcag tgtttaaatc
     3721 tttgtgggat cagggcatta tcttacgtga tcagaataaa caaccctctt taagcggctg
     3781 cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc gtcattgacg ccttacgtgc
     3841 ggagcaagtt tgatgagtca gaagtatctt tttatcgatc gcgatggaac cctgattagc
     3901 gaaccgccga gtgattttca ggtggaccgt tttgacaaac tcgcctttga accgggcgtg
     3961 atcccggaat tgctgaagct gcaaaaagcg ggctacaagc tggtgatgat cactaatcag
     4021 gatggtctgg gaacacaaag tttcccgcaa gcggattttg atggcccgca caacctgatg
     4081 atgcagatct tcacctcgca aggcgtgcag tttgatgaag tgctgatttg tccgcacctg
     4141 cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac tggtggaggg ttatctggct
     4201 gagcaagcga tggatcgtgc caacagttat gtaattggcg atcgcgcgac cgacattcaa
     4261 ctggcggaaa acatgggcat taatggttta cgctacgacc gcgaaaccct gaactggcca
     4321 atgattggcg agcaactcac taaacgagac cgttacgccc atgtagtgcg caacaccaaa
     4381 gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag gtggcagcaa gattaacacc
     4441 ggcgttggct tctttgatca catgctggat cagatcgcca cccacggcgg tttccgcatg
     4501 gaaatcaatg tcaaaggcga cctctatatc gacgatcacc acaccgtcga agataccggc
     4561 ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca aacgcggtat ttgccgcttt
     4621 ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg cactggatat ctctggtcgc
     4681 ccgcacctgg aatataaagc cgagtttacc taccagcgtg tgggcgatct cagcactgaa
     4741 atgatcgaac acttcttccg ttcgctctct tacaccatgg gcgtgacact acacctgaaa
     4801 accaaaggta aaaacgatca tcaccgtgta gaaagcctgt tcaaagcctt tggtcgtacc
     4861 ctgcgccagg ccatccgcgt cgaaggcgac accctgccct cgtcgaaagg agtgctgtaa
     4921 tgaacgtggt gatccttgat actggctgcg ccaacctgaa ctctgtgaag tctgccattg
     4981 cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga cattgtgttg ctggccgata
     5041 aactgttttt acccggcgtc ggcaccgcac aagcagcgat ggatcaggtg cgtgagcgcg
     5101 agctgtttga tctcatcaaa gcctgtaccc aaccggtgct gggcatctgc ttagggatgc
     5161 aactcctggg acgacgcagc gaagagagca atggcgtcga cttgctgggc atcatcgacg
     5221 aagacgtgcc gaaaatgacc gactttggtc tgccactgcc acatatgggc tggaaccgcg
     5281 tttatccgca ggcaggcaac cgcctgtttc aggggattga agacggcgcg tacttttact
     5341 ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat cgcccagtgt aattacggcg
     5401 aaccgttcac cgcggcggta caaaaagata acttctacgg cgtgcagttc cacccggagc
     5461 gttctggtgc cgctggcgct aagttgctga aaaacttcct ggagatgtga tgattattcc
     5521 ggcattagat ttaatcgacg gcaccgtggt gcgtctccat cagggcgatt acggcaaaca
     5581 acgcgattac ggtaacgacc cgctgccgcg cttacaggat tacgctgcgc aaggtgccga
     5641 agtgttgcac ctggtggatc tgaccggggc aaaagatccg gctaagcgtc aaatcccgct
     5701 gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag gttggcggcg gtgttcgtag
     5761 tgaagaagac gtggcggcgt tactggaagc tggcgttgcg cgcgtggtgg tcggttccac
     5821 cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa cgtttcggtg ccgatgcgct
     5881 ggtgctggca ctggatgtcc gtattgacga gcaaggcaac aagcaagtgg cagtcagcgg
     5941 ctggcaagag aactcgggcg tttcactgga acaactggtg gaaacctatc tgcccgtcgg
     6001 cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc acgctggcgg gatctaacgt
     6061 ctctttatat gaagaagtgt gtgccagata tccgcaagtg gcgtttcagt cctccggcgg
     6121 cattggcgac attaacgatg ttgctgccct acgtggcact ggcgtgcgcg gcgtaatagt
     6181 tggtcgtgca ttactggaag gtaaattcac cgtgaaggag gccatcgcat gctggcaaaa
     6241 cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg tgaaaggcgt acagtttcgc
     6301 aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac gctacgccga agaaggtgca
     6361 gatgaactgg tgttctacga tatcaccgct tccagcgatg gccgtgtggt agataaaagc
     6421 tgggtatctc gcgtggcgga agtgatcgac attccgtttt gtgtggcggg tgggattaag
     6481 tctctggaag atgccgcgaa aattctttcc tttggcgcgg ataaaatttc catcaactcc
     6541 cctgcgctgg cagacccaac attaattact cgcctggccg atcgctttgg cgtgcagtgt
     6601 attgtggttg gtattgatac ctggtacgac gccgaaaccg gtaaatatca tgtaaatcaa
     6661 tataccggcg atgaaagccg cacccgcgtc actcaatggg aaacgctcga ctgggtagag
     6721 gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca tgatgaatca ggacggcgtg
     6781 tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg aagtttgcca cgtcccgctg
     6841 attgcctccg gtggcgcggg caccatggaa cacttcctgg aagccttccg cgatgccgac
     6901 gttgacggtg cgctggcagc ttccgtattc cacaaacaaa taatcaatat tggcgaatta
     6961 aaagcgtacc tggcaacaca gggcgtggag atcaggatat gttaacagaa caacaacgtc
     7021 gcgaactgga ctgggaaaaa accgacggtc tgatgccggt gattgtgcaa cacgcggtat
     7081 ctggcgaagt gttaatgctg ggctacatga acccggaagc cttagacaaa accatcgaaa
     7141 gcggtaaagt caccttcttc tcgcgcacta aacagcgact gtggatcaaa ggcgaaacct
     7201 cgggcaattt tctcaatgtg gtaagtattg ccccggactg cgacaacgac acattactgg
     7261 tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac cagtagctgc ttcggcaaca
     7321 ccgctcacca gtggctgttc ctgtatcaac tggaacaact gctcgccgaa cgtaaatatg
     7381 ccgacccgga aacctcctac accgccaaac tgtatgccag cggcactaaa cgcattgcgc
     7441 agaaagtggg cgaagaaggc gtggaaaccg cactggcagc aacggtacat gatcgctttg
     7501 agctgaccaa cgaggcatct gacttgatgt atcacctgct ggtgctgttg caggatcagg
     7561 atctggattt aacgacagta attgagaacc tgcataaacg gcatcagtga gttacgggct
     7621 gagcaggtgt gatgttgttg tcacacccgg agccattgcc cgatgcgcaa acttatcgtg
     7681 cctacacatt atctcaacac acgtaggccg gataagatgc gccagcatcg catccggcaa
     7741 aaaaaacggg catggtgtca ccaccctgcc cgtttctctt aaatgcacaa taatattact
     7801 tcgcgttgta attacgcaga gcgttacgcc ccaacacaat ccccgcgcca accatgccgc
     7861 ccagaagcac tgccagaatc aaggtgattg cctttttcgg actatcgcga cgaataggta
     7921 acgtcggttt catcacatag cggtaagcat gaatatcaag atcatcaaac tttaattttt
     7981 caatatccaa caggttttga cgtgtctgat agtagtttgg tgagaacacc aacggacgag
     8041 tcgcttcatg cttaatcatc gactccagcg cttcgctccc tagaaggaac aacgtatctt
     8101 gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac ctgcgcctga ttcgcatact
     8161 gcaacgcttc ctgaatctga cggatacgca gatctttctg ctcctgcgcg accacttcct
     8221 gggttcttaa agagtcctgc aagtttttcc gtcccagagc aatgttgtcc ttgagatcct
     8281 tttctagctc ttgattcact ttatcatcaa cttgctgaat gtattgggcc aacttcattt
     8341 gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa tggtaattgc tggttcttaa
     8401 cagaaggttc gatggtaagt ttttctggct cttcctgatt atccagcgtt tctgctaatg
     8461 cagagaaggc agaactgaag cgaccaatta acgtctcctg caaatccgat actttcggtg
     8521 cagcctgacc atagataaca ttcatggcat tgttatagcc agcaatttgc cccacgtcgg
     8581 gctgagtgat aattgctgtt gacgtccatt tctccttcgc tactgccaaa taaccaatag
     8641 ccagggcaat agccacaatg acggaaatga taattgtcat cttgccacgc cacaactgca
     8701 ctagtaaatc aatcaaatca atctgttccg ggtcatggtt ttgcccagaa acattattat
     8761 tttctactct catcattacc ctaactgacg gaaatttcca agtccgcata gtttagcgat
     8821 aatccccgtg aatgctatag gataagtgat aaaaataatg aattattgcg gaggtaacaa
     8881 aagacaatca gggcgtaaat agccctgata acaagatgtt agtcgctgcc aaagagatcg
     8941 cgggtgtaga ccttatctgc cacatcctta agctcttctg ccatacggtt ggagataatg
     9001 acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca ggcgagagtt gaagaatgag
     9061 tcttctttca tcaccggctc gtagatgatc acttcaacgc ctttcgcctt gatacgcttc
     9121 ataatcccct gaatagaaga cgcacggaag ttatctgacc cgctcttcat aatcagacga
     9181 taaataccca ccacttgcgg tttgcatcga caattgccga gatcaggtta ttcggcacag
     9241 actggtagtt cgccagtaac tgcttggtat ctttcggcag acaataacca ccataaccaa
     9301 acgacggatt gttgtaatgg ttgccaatac gcggatcgag acaaacgcct tcgataatct
     9361 ggcgagtatt cagacctaaa ctttctgcat aactatccag ttcattaaag tatgctacgc
     9421 gcatcgccag ataggtatta gcgaaaagtt taatcgcttc tgcttcagtg gagtcggtaa
     9481 acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa caacgcagca aaacgttctg
     9541 cgcgttctga acgctcaccg atgacaatac gtgacggatg gagattatcg taaagggctt
     9601 taccttcgcg gaggaattcc ggagagaaaa taatattttc agtacgatat ttcttatgca
     9661 tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac cataaccgca taaggattta
     9721 tctcaactac gtctttaatt actgattcta cactggatgt attgaaataa ttagttttag
     9781 gatcatagtc ggttggagtg gcgatgatga cataatcagc atcccggtag gcttcatttt
     9841 tatctaatgt ggcattaaag tgtattttat ctgattgcaa aaactgctga atttccttat
     9901 caacaatagg agatatccga tcattcagca tagcaacgcg tgacggtaaa atatctaatg
     9961 ccacaacctc atgattttgt gcgattagaa gcccgtttga caagcctaca tagccagtac
    10021 cggaaatggt gattttcatt tcatgctctc agaattaact taactgtgaa tcatgatgtt
    10081 tttagcatcg tgataagatg ctaaaagttt taacgctacg ttgtcagatg gtcaacgcaa
    10141 cgaaaaacaa ttcagatttt ctttataaga atagctgatg tttattaagt taatattaag
    10201 cagtgaaatt tagtctataa gatatttggc aaaaaaaagc ccggtgcaat atacgccggg
    10261 cctcaatttt attgttggtt aaatcagatt aatccagcca ttcagtatgg aacacacctt
    10321 ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc acgctgtgcc tggatcaggt
    10381 tcgcaggcag aaccgcggca cggtagctat cgtaataggc aaccgcagcg gcgaaggtcg
    10441 gaaccgggat accgttctgt actgcataag cgacgacatc gcgcagcgcc tgctggtagt
    10501 catcggcaat ttgcttgaag tacggggcca gcagcaggtt agcgatctgc ggattttcgg
    10561 cataagcatc ggtgattttc tgcaggaact gcgcacggat gatgcagcca gcacggaaaa
    10621 tcttcgcgat ttcgccgtag ttcagatccc aattgtattc ttcagacgca gcacgcagct
    10681 gagagaagcc ctgagcgtaa gaaacgattt tgcccagata cagcgcactg cgaacttttt
    10741 cgatgaactc agccttgtcg cctgctgact gagcttgcgg accagagaga actttagatg
    10801 cggcaacgcg ctgatctttc agagaagaga tataacgtgc aaacacagac tcggtaatca
    10861 gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt ccatttaccg gtgcctttgt
    10921 ttgctgcttc atccagaatc acatcaacca ggtagttacc gtcttcatct tttttggtga
    10981 agatatcttt agtgatgtcg atcaggtagc tgctaagttc accgttattc cactcggtga
    11041 aagtctgggc cagttcttca ttagatagat tcaggccacc tttgagcaga gaatacgctt
    11101 cagcaatgag ctgcatatct ccgtattcaa taccattgtg aaccatcttc acataatggc
    11161 ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc gtcttctgcc acggcagcga
    11221 ttttagtcag gataggcgca accagctcat aagcctcttt ctgcccgcca ggcataatag
    11281 aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc ggtaccgatg aagttaaagc
    11341 cctctgctga aagctcacga ttacgacgaa tggtgtcctg gaagaaggta ttaccaccat
    11401 caatgatgat gtcaccttta tcgagatatg gtttcaggga atcaatcgca gcatccgtgc
    11461 ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt ttccagagat tcaacaaact
    11521 ctttcaccgt atagtaagga gccagtttct tgcctggatt ttcagcaatc acttcttccg
    11581 tcttttcgcg ggagcggttg aaaatagaga cggtataacc acgactttcg atgttgagcg
    11641 caaggttgcg ccccatcact gccataccga ctacgccgat ctgttgcttt gacattgttt
    11701 actcctgtct tctatattat taaccgtcta ttaaccgtta tattattaac cgtcgtgatt
    11761 ttcatcttct aatcaaggaa tacatatggt tacagcatgc ttcttacttt atagcgataa
    11821 gtatgccatt gggtgtatcc ataagaactt tgaattttgg atacttttct ggattaaaat
    11881 tagcatagct tcgtttagat ataacaataa acgaactatt aaaagatttc atcttctcta
    11941 ttaaatcatc ctgtgggatt acctcacact cgggattact aatagtccaa tcactatatc
    12001 ttgctaatcc aaagacgtca ccaattgcca tccctttata gaagtaaata ccatcctcca
    12061 agcctaattg gataagatta tctccatata actcactata ttcacttgct ttcttcatca
    12121 gttcatagct atagcgactt tctaagcgtg aattaaaatt caagtattca gaataaaaat
    12181 atttgaattc aaaatacaac gatcgccccg aaaaaaaata atacacacaa ggataaaata
    12241 tcgtcccaac ttgtaatttg taatgttgat atcttttatt aaacttgcaa ttgagatcgc
    12301 aacctgcaat actgacaatg ggtaaatggg tgataaatac ctgtcacctt gcgtaacaga
    12361 aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat acccaaagta ttttattatt
    12421 cctaagtgta aagacagaca gtatagcata aaaaacaaca tcactgccaa ctttattaat
    12481 tgcttgttgc aaatctaatg tggcaggact tataccatgt cgagcttgtt ctgctgtttg
    12541 aacttgtaga tcaatttcat tccataggta gtgaccaaat atatttcctc cagcaggact
    12601 aaaaggatca ccagagatcc aaaaactacg gacataccaa taaatgccac caagaattgt
    12661 tgccaagcta aaaataagaa atgtctgtat gtttctcttt ttcgctatca atataacaaa
    12721 tatgatggct ccatatatgg caccaaatag ttttacgcca caagcaattc caataaaaaa
    12781 accacataaa aaaattaatt cattttgtgt tttattgttt tctgagcgaa tatatataag
    12841 aatactggct gcagcaaaac aaagatagcg ttaaatcaat atatgcataa ccaagatgat
    12901 ctttaagtgg attgatagta aatagtagga ttgtagcaat aatgcctgga taataagttt
    12961 tcgtaatttc ccatgaacag ccaacaagtc cacaggctat tacaaaaaaa cagatatttg
    13021 ctaaaaactg agctaacctt acatctccta actgtaaacc taaagccatt aaaagatctg
    13081 cattttgcgg gaacagagga aaacgcaaat attgttcaac aacaattgca tggtgctcaa
    13141 ctaatgaacg agccaaaggt aagtgataca tagtatcatc ccaatcaccg ggagctcgca
    13201 gggacgataa caatgtaacc acaaaaatca aaacaagaaa taaaataaca aacagcgtac
    13261 tgaactcaat attatttatt gccagccatc taaattgctg acgtttaata atgcttaaaa
    13321 cccaacacac cgtaaaaatt aaaaagatag caatcttatt aaattgcccg accatgccca
    13381 gaaagtaaat tatcaaaata tttaaagaca tccctatgca taggcaataa aaaataaaga
    13441 aaatttgtta agtcctgcca ccccctcctt aaaaaaagat gacaatacaa tattaccaat
    13501 gacaaaaaat gtcattccaa aagcaaaaca atacacaaca tacaaataca acggcaattc
    13561 aatcatagta aaatactcta tttacattat cagtttatag tttttttcag aaagataaaa
    13621 taatgccttc atttcacgtc tagttgttgt atttgaatgc aaaataatgc cacatacaaa
    13681 tgccattatt gacgataaca tgatggaggc agttaaaaag gcagtcggaa atcttctcac
    13741 caaacctgta tcaatataat caacaatgat gggcaatcct aaaattatag acaaagcagc
    13801 aagtaaaacg ccgataatac taaaaaagta tagtggccgt tcaagcgagt aaagtttaat
    13861 tattgttttc agaatcttga acccatcact ccaagttgat agtttgctca cactcccttc
    13921 agatcgctcg ccatattttg tattaacctc accatacttc atacgtaatt ctaaagcatg
    13981 aattgtaagc tcagtttcga tttcaaatcc ccttgataga catggaaaag atttaacata
    14041 gcgtctggaa aatacacgat accccgacaa catatcagta aaattccctt taaagatagt
    14101 ctgaacagta ccagtcaaca atttatttcc tcagcgatgc ccttttctgt atgtattttg
    14161 atctccattc tcttgacgac atcctacaac catatccaat tgatttttta tgagatggtt
    14221 aatcatttct ggagcagaac ttgcatcata agtgtcatct ccatcaacca ttagataaat
    14281 atccgcatct acatctgaga acatacgtct tacgacattc cccttgcctt tcagaggaac
    14341 agagtgaact gttgctccag catcctctgc cacttgtgct gtactgtcag tcgaattatt
    14401 atcaaataca tatatagaag cagttggaat attcgttttg aaatcattta tgacttttgc
    14461 tattgccttc tgctcattat aacaaggaat aattacagcg atattaaaag agtgatacat
    14521 aagcccctca tataaactat ttagctaatg tgcaagtaat tgtgttttta acaaatcgtt
    14581 tactaacgaa aaatgaaata ttttgatgcg caatatccta aaaccaaact aaaaagagaa
    14641 aaggtgaaca atgtaaatat tggagataaa gctaagagat caaataaaac gccgaataaa
    14701 taactcatcg cccccataaa aaatataaat ataaaatatc tagccttggt tgcagatact
    14761 ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg ctgcacatat aaaaccaaca
    14821 atgttacttc ggccttgacc aaaagctaaa ttattataac acgcataaaa taccccccaa
    14881 tgtaaagcag tattgacaat tccaattaaa aaataactaa aaaaagaact ggtcaataac
    14941 ttccctattt ttaacatcct ttattttgct ccagaagtga ggttattact aatttggata
    15001 ttttctatag aaaataccca taaaactaca acgcctataa taaaaattcc catgggtgta
    15061 aaaagaaatg gattagttat agaaacggta atactgaaaa aaagtattaa cgacatcatt
    15121 aatgatgttt ttgtactatt taaaaaatag cctttcaaaa agataagcaa cataggaact
    15181 aatgatataa atacaccaat aacgcctgtt ttctgaagta tctcaagagg tactatttca
    15241 atccttaatc gatctaatat aaatgaacca aatcctcttc caaacaagaa cgtcgctaaa
    15301 tcaacatttt tataataaaa atataaatca ttaaatctaa cagagtcaga atcttcactg
    15361 cgtgatccga ccatgtaaag cataattata aatagaacgc tgcccaatat tattataata
    15421 gtatttaaat atagcttatg taatttaaaa ctggctaata acagtgataa tgttgtaaaa
    15481 agtaatacac ctcttgattc tgtcaataat atcgcaagca ctattatgag acattttaaa
    15541 tattttttgt cgacaaaact gataatcgca ccgacaccaa aaaagtagaa ggatttggaa
    15601 aaaaaagccc catcgggacg aaaaaagaac tcgctagtca atgataagtg ttcataaatt
    15661 aaattaaaat ttaacaaacc gaaattcaac aaaattatgt atgaaatata taacagtgat
    15721 ccatataatg caaatgcatt aactatttta ataaaaatct cacatgaaaa tacttttaaa
    15781 gtatatctta gtgtaaatag atatataagt gacagcataa aaaggtatgg ttgcacatca
    15841 aacagtatat catttatttt attaccagct aataagccga caaagaagcc ataaaaagga
    15901 aataaaagca accatataaa aagactgaca catttttggg tgattgaaaa attgaatgta
    15961 actaaattaa aaattaaaag tataaaacta aatacaaata ataaatatcg caaagggaat
    16021 attcccaatg gctccagtaa tcttccacca ccaccaataa tcagttcaat acataaaaat
    16081 gttataaaaa ttttatttat attattcata ttcgtaaggt gatgtttttt aaaatatgtt
    16141 taaaatcaac attaataaaa tataaatttt atcagccttt ttccgcctta acttccaaaa
    16201 acttaaagcc aagatcaaat tgcctatata acctttaccc aattgattat tcacaaagct
    16261 atcatcagct aaggcattag ctattttagt tacttctttt cgataccact tgtttctcac
    16321 caaaccaagc cgtttgtatt ttgctttaaa actaatgttt gctcccactt ggttgttttc
    16381 atgctgtctg tacagcattg ttggttttct atcaatatac cacgaataat ttctcgtccg
    16441 cgcaaatgaa tataaaaacc aatcatgcag acagatatct tgtgcagcat ttttattatt
    16501 tatgatgaac ttcttaaatt caataagcgt ttcttttttt ataacatatg tacaccccgg
    16561 acctgctgct tcaaaaaaat aatcgaattg agtttgcgac tgagccttat caactaaatt
    16621 ttttcgccct gatggataat atgcaattac attgctggaa taacaattcg cattattaaa
    16681 taccaagcag tcaattgcat tttttatttt atctttatac caaatatcgt cctgatcact
    16741 aaaacagata taatcatagt tttcaagatc gacatcttta attaatctat aaaaattttt
    16801 acctgccccc ccaaacttat tgacgcttgg aagtatattt ataattgagg gatatgataa
    16861 ttgaaattct tcacaaatat ttaatgtatt atcagttgat aaatcatctg atataaaaat
    16921 tgagatgtct acatcttttt gttcaataat actttttaat tgttcctcaa tccagcattc
    16981 gccattatac gtagccataa gtacagctac tcttttttga tcagaactat gcattataac
    17041 gaccgccccc agtaattcct cttattccat ccagcgtagc caaaatatgt tttttcatat
    17101 attcaatttt atttttttga gttgcgataa ttaaagtcgt atgaattaaa gaaaaaacaa
    17161 cttccctaat agagagtaaa agaggcacat gattttttct aagcaaaaga aatgcatttc
    17221 ttactcgata ataacgacgt actggcgagt gaattggtat gttaatccca cagattttta
    17281 ctgacttatc cccaatagca tgaaccattt ctatatcagg aatgatatga actaatatgc
    17341 catagtttaa gcatctaaga caccattctg tatcgacata atcaataaaa agttcctcat
    17401 ccatcattcc aacaatctca aaaacttctt ttgaaaccat ggttccggat gagattgtta
    17461 ctgaactttt aaacggtgtc tgtccttcag tgacatgaat tttctctcga agaccatttt
    17521 ttttgatatt acaaatagga taataatgtg acttatctct atcaataaaa actgggccag
    17581 ctatttttgc attttcatta tccatatagc aggaaagttt tttgataaac tcattgcgaa
    17641 tacggctatc ttgatcaaaa aaaattatct tctctgcccc tttttcaaga gaatatcgaa
    17701 tcccaatatt ttgtgctttt gctatacctt cattcctacc aagacaaatt aaatgaactt
    17761 tttcaatgtc tgctaattta tctttcaagg tgaaattagt cctattatcg actactacaa
    17821 cgtaacaatt ttgctccttt agttcagtga tcagtgcatt cagattttta agttcgggat
    17881 tatatgtcac aatgacagcg taaatattac tattcattat ccaagtgact cagtaattgg
    17941 ttaattttag ttttcatata cagaacacat tctagtataa agccattgta ataacagagc
    18001 atctaaaatt acacgtatgc tccaggatat tgcggcaccc agtaacccat atttttcgac
    18061 cataaaatac aaaagggcta aataaggaac caattcagca caatgaatca aagcagtaat
    18121 tttagatttt cctaatgcct ggattgcaga gaatggtatc attgccagcg cattgaagaa
    18181 aaaaccgatc aataacacat ttaatatatt aaaggaatta atttgataat tctcaccaaa
    18241 ccatattttc aatacaagcc ctgaatataa caagcctatg attatcaccg gcaaacagat
    18301 tagaaacatt aaaagcaatg atttatttac attacgttta aattctttaa agtctttgat
    18361 gttacttaac cttggaaaca ctgccctccc gattgctgca ggtattattc ctaattttag
    18421 aataacctcc gctggtgcag aataaaatgc aactttatct gcccccataa tatttgacac
    18481 tataaatcga tcaaaataca ccatgaccgg acttatgata ttacttactg tcatccaacc
    18541 gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa tgaacccccg aaattttaat
    18601 atcattcctg acataatagg cagagactaa aatcgaaata actcttgcaa atattaaacc
    18661 agcaaccgct gccgacaatg tagcactata aaaaacaaat atggccggaa ttcccgcgat
    18721 gcaagagctt gatatagatt tttgaatatt tacaatgcca aatttttcat ccccctcaag
    18781 aatggctgac cataattgat ttagaataaa taatggaatg caaatagcca acagtttaaa
    18841 tgctagttgt atatcactat gttcaacacc agaaattttt agataattaa caattccatc
    18901 agagaaaatc aataataaaa aagcaccaaa gcatgaaaat agcaccaaaa aagatgtact
    18961 tgttgaaatt accttggttc tttcatgatg attatcacga tgaattgcaa tttctctaat
    19021 aacagagcga gtcaggccta catcaaaaat tccagcatac ccaacaagtg caattgcaat
    19081 tgtataaaca ccgaaacctt caggccccaa acctcgagcg agaaatccta gcgaggggat
    19141 agcaattaaa gttggtattg catagccaaa aaggttccag acactatttt ttattatgct
    19201 cattattaca tttctttatc atataaaata atcagcatct acaaattttt gaccattaat
    19261 atctttatca gaaagcaatg gattttgaac cggccattct atatttatac ttttgtcgct
    19321 ccataaaatc ccacgctctt gcatttttga atagaaatta tttgttttgt agacaaattc
    19381 tgcttcatca ctaagaacgc aaaacccatg agcgaatcct tccggtatcc ataattgacg
    19441 cttattctcc gccgacaaat ttacaccaac ccacttgcca aatgttggtg atgatttccg
    19501 aatatcaaca gctacgtcaa aaacttcacc gacaacacag cgtaccagtt ttccttgagc
    19561 ataaggttcc agctgatagt gcagtccgcg taacacaccc ttagttgatt tagaatggtt
    19621 atcctgaaca aactcaacct ttcgtcctac agcctcttcg aaaactttct gattgaagct
    19681 ttccataaaa aagccccgct catcaccaaa tacttttggt tcaaaaatta gcacatcagg
    19741 aatttcagtt ttaattacgt tcattttatt aataaccttt aatcattttc agcagatact
    19801 gaccataagc atttttcttc agcggctcgg ctaatacctt cacttgctca gcatcaataa
    19861 agcctttacg gtgagcaatt tcttccgggc aggaaacttt cagcccctga cgctcttcaa
    19921 ttgttgcaat gaagttgctt gcttcaataa gactttgatg tgtccccgtg tccagccatg
    19981 cataaccacg ccccatcata gcgacagaca aacgtccttg gtccatataa atacggttaa
    20041 tatcggtaat ttccagttcg ccacgtgcag atggtttaag gtttttcgcc atttctacaa
    20101 cgtcattgtc atagaaataa agcccagtaa ccgcatagtt actttttggt tcctgcggtt
    20161 tttcttccag actaattgcc gtaccgttat catcaaattc caccacaccg tagcgctcag
    20221 gatcatttac gtgataggca aaaaccgttg cgccactctc tctgttaaca gcggtatcca
    20281 ttaatttcgg caggtcgtga ccatagaaga tattatcacc aagtaccagc gcacaatcat
    20341 caccacctat aaattcttca ccaatgataa atgcttgcgc cagaccatcc ggactcggtt
    20401 gtactttata ttgcagatta agcccccact ggcttccatc accaagcaat tgttgaaaac
    20461 gtggtgtatc ctgcggcgta ctgataatca gaatatctcg aatacccgcc aacataagcg
    20521 ttgaaagcgg ataataaatc atcggtttat cataaatcgg tagcagttgt ttgctcacag
    20581 ccattgtcac aggataaaga cgagtgccgg aaccaccagc cagaataata cctttacgcg
    20641 ttttcatttc accattcctt ttaattcatc tcgctctagc atcatgagcg agatgcaata
    20701 atttgttaaa ttgccgtggt cgtaaataat tcgttgagca tacgtttcac gcccacctgc
    20761 cagtcaggca agacaagcgc aaagttctgc tgaaattttt cggtattgag gcgagaatta
    20821 tggggacgac gtgctggtgt gggataggcc gttgtcggca cggcattaag tttgttaaga
    20881 gcaaggttga tccccgctct gcgcgcttct tcaaatacca gagcggcata atcgtgccag
    20941 gttgttgtac caccagcaac cagatggtac aaaccagcaa cttctggttt atttgcggcc
    21001 acgcgaatgg catgagcggt gcaatcagcc agtaattcag cccccgttgg tgcgccgaat
    21061 tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca ggcgcaacat cgttttggca
    21121 aagttatttc ctttaccagc gtatacccag ctggtacgga aaataagatg ctttgcgcaa
    21181 tgttcttgta acgctttttc cccagctagc ttggtttcac cgtagacatt tagcggtgct
    21241 gttgcatccg tttccagcca tggcgtgtca ccgttgcctg ggaaaacata atcagtggag
    21301 tagtgtataa cccaggctcc gacttcatta gctgcttttg caatcgcttc gacgcatgtc
    21361 gcgttaagta attgcgcgaa attcggttct gattccgctt tgtctactgc agtgtgagca
    21421 gcagcattaa caataacatc ggggcgaatt tttttgacgg tttcagccac accttcgggg
    21481 ttgctgaaat ctccacaata atcagtggag tgaacatcga gcgcaatcaa gttacccaga
    21541 ggagccagag cacgctgcag ttcccaaccc acctgccctg ttttgccgaa aagcaggata
    21601 ttcattactg acggccctca tagttttgtt caatccacga ctgataagta ccgcttttca
    21661 cattctcaac ccaatttgta ttagccagat accattccac cgttttacga atcccgctct
    21721 caaacgtttc ttgcggtttc cagcccaatt cgcggctaat tttatcggca tcaatggcgt
    21781 aacggcggtc atgccccggg cgatcggcaa cataagtaat ttgctcacga taagatttct
    21841 ctttcggtac aatctcatcc aacaaatcac aaatagtgag cactacgtcg atgtttttct
    21901 tttcgttatg tccaccaatg ttataagttt cacccgcttt accttcggtt acgacggtat
    21961 ataacgcacg agcatgatcc tctacataca accagtcgcg aatttgatcc cctttaccat
    22021 aaataggtaa tgccttacct tccagagcat tcagaataac caatggaata agtttttccg
    22081 ggaagtgata aggaccataa ttgttcgagc aattagtcac aatggttggt aaaccataag
    22141 tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt agacgcagaa tacgggctac
    22201 ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc ttcgttatta tttgcttcat
    22261 ccggatgggg taagtcacca taaacttcat cagtagaaat atgatgaaag cggaaacttt
    22321 ttttcttttc atcattcaga gcagaccagt aattgcgcgc cgcttctaaa agaacataag
    22381 tccccacaat attggtttca ataaatgccg cagggcccgt tattgaacgg tcaacgtggc
    22441 tctctgccgc caggtgcatc accgcgtctg gctggtgctg tgcgaaaata cgcgacatgg
    22501 ctacagcatc gcagatatct gcatgctcaa aagcataacg ttcagaatca gaaacatcag
    22561 cgagtgattc caggttcccg gcgtatgtta atttatcgac attaacaaca ctatcttgcg
    22621 tattatttat tatgtgacga acaacagcag aaccaataaa ccctgcgcca ccagtaacaa
    22681 gtatcttcac ttttttattc catatagcta tagagcatgc tgtgaaatag actgctccag
    22741 atttgattaa tagaggtatt aatacacgct accgcccctg gcttaacagc taccagtgca
    22801 ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg cgccaaatcc gaaaaattca
    22861 aacgttaatt gtcttaccaa acagccgtag aaacaaggaa aatcctggaa aaatttgaat
    22921 aatatgagct atgtaacttc ttgtttttat aggctttata aaaacggcaa cggcaggaaa
    22981 attcaatgag caaaacttat gccacgaatc ttcactgccg ttataatttt cttatcaacc
    23041 gttacatccg gtcagatttt cattattcgc ttaacagctt ctcaatacct ttacggaact
    23101 tcgccccttc tttcaggttg cgtaggccat acttcacaaa cgcctgcata tagcccattt
    23161 ttttgccgca gtcgtaactg tcgccggtca tcagcattgc atcaacggat tgttttttcg
    23221 ccagctcggc aatagcatca gtcagctgaa tacgtcccca tgcaccaggc tgagtacgtt
    23281 ccagttccgg ccaaatatcg gcagaaagca catagcgacc tacggccatg atgtctgagt
    23341 ccagcgtctg cggctgatcc ggtttttcga taaattcaac aatgcggctg actttaccct
    23401 cacggtccag cggctcttta gtctggatga cggagtattc agagaggtca cccggcatac
    23461 gttttgccag cacctggctg cggcccgttt cgttgaaacg tgcaatcatg gcagcaaggt
    23521 tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac gtctggcagt accacgacaa
    23581 atgggttgtc accaatggca ggtcgcgcac acaaaatgga gtggcctaaa cctaaaggtt
    23641 cgccctgacg cacgttcata atgatcacgc ccggcggaca gatggactgt acttccgcca
    23701 gcagttgacg cttcacgcgc tgctcaagga gtgattctaa ctcataagag gtgtcgaagt
    23761 ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag gatttctttg atccctgcag
    23821 ccacaatctc gtcaacaatg tactgaatca ttggcttgtc gacgattggt agcatctctt
    23881 tgggtatcgc cttagtggca ggcaacatat gcatcccgag acccgctaca ggaataactg
    23941 cttttaaatt cgtcattatt tcatccacct gtaaaatggt tgctgaatta tagcttgttc
    24001 gatttttttc gccagcatca attaccctga attgattact gaattacttg tgatgttacg
    24061 ccgcttcgtt gtggattgca gtagcattgt tcctaagtat ggctccattt ttccaggaat
    24121 ggtcgcaaat ctactccctc cgttccggca atctaaagtt aatcttttcc acattaacaa
    24181 tatggtgatt aatcctgtcg atatcgacgg agctttgccc tctttcattc accgcatgaa
    24241 catttgcaag agacagcagt gtttcttttt tcgccataaa gacgccgcga acgtctttgc
    24301 gcatgtcgaa gttcatgctc aatgctggtc caactgagga ttcctgcatc acattgatat
    24361 ttcgcataaa aagatgttgc ggcttgttgt gtaactccag cgaggcacgc ttcatctcaa
    24421 tgttagttag cgacacaaag gagacggcat tcccggcgga gatttggatg ccgcgcaatt
    24481 tataagcaag atgggtatta tccagttgaa tattattcac tcggaaattt tgcggtatcg
    24541 agagatattt gcctttaatt accccatagc cgattaacat cccggcgcta ttaatcattt
    24601 caatattatc aatcacgaaa ttgtcacaac cgtaaatagc gactgtcgcg ttatcaatgc
    24661 ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt gatattacga ataacaaaat
    24721 gtttaccatt ttcaacatgg atcaactgcc gacaatccga tcccgtgata ttcgccacga
    24781 caaagttttt cactgcctgg tcttccgggt agttgttatc ataagtgctt cccgcaaggc
    24841 ctatgccgat gccccagttg attttgccgt tggtacagtt gatacgctcg atgacatggt
    24901 cagatatcaa aatatcactg tcgttaattg ccacgttcca ttcaatggcg tcgccctgta
    24961 agtcgctgaa cttacagttg gtaatgttgg caccgataat ctggttatga aatccctggc
    25021 gtaagatggc gtaattagcg tggctgacag tgaggttatc gatggtcagg ttgcgcatga
    25081 cccgtttgtt tttgccgcca atataaatct gcattaccgg gacaaagccg ctcatcgcca
    25141 gccctttgat ggtgcagtca gaaccacgca catccagggt aatgttatgc ataccgccgc
    25201 cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa tcgccctctg ccgttgccgc
    25261 gcaggcttcc aaggatgtgt aacgttttgc cgggagggat gaagatgccg gtgttgatat
    25321 tgtcacaaac gaatccggca ggcacgacga ccgtttgccc ttcgctgaag gcttgtttaa
    25381 atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt aacgctttgt cgggcgggaa
    25441 gtgcgcgagc gaaaggggta tggaggaagg caagcgccga gcttgccgtc aggaaggtgc
    25501 gtcgggagag tgttttaaat ggcatacgtt ctcctctata aagcctgcag caagctggcg
    25561 agttctcgat taatcacctg ctggttaaaa tcgtgttcga ctttttcgca cgcacgtttg
    25621 acgaccggag ccagttcgtc ggtatccagt tggctaaacg ccgccagacg ttgtgccagt
    25681 gcgcgagcat cgttctcagg cagcagccag ccggatttat cggcctccac cagttcagga
    25741 attccgctat gcagagtaga aaccaccgga ataccgaccg ccatcgcttc cattagcgcc
    25801 accggaatgc cttccatatc gccatccgcg cccgttaccg atggcaacag gaagacatcc
    25861 gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa agcccggcat ctctaccaca
    25921 tcttccagtt gatattgttc gatgatggtg cgcaggcgtc gttcccacag gccaatgccg
    25981 aggatgcgat agcgaaatgc cacgccctgc tctttcaact gacggcaggc ttcaatcgcc
    26041 acatgcaggc cttttttctc ggttaagcgt gcgacagata tgatttccag cggcgttgcg
    26101 ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca cgcccatacg cgatacggcg
    26161 attttttctc tggggcagcc cattttttgc agccttccgg cccacagatc gcttatcggt
    26221 aacatcaggt cgccacggcg aaacagttgt tgatattcgg gagtgtagtg gttgagcact
    26281 tcccgactgg agatatcaat accgtggaag atggtggcaa ttttgccgcg aatgataccc
    26341 agttcgcgta gttttgctgc ggttaccccc gccgggccaa aatgagcgat gaacacatcg
    26401 gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca aaatcaggtt ccgcgactcg
    26461 gcaccatagc gtttgaggtt gagcgcatgc caggtatttt tacgatgaat gccacgtaag
    26521 gtctggctgg cgcggtggcg cagtttcgct actttgcccg caggttcgtc ctgtaaccag
    26581 cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt gggtgttctc tgtatcgcct
    26641 ttttgcaaag cgacaatctc cacttcaaag cccatatcaa taaacgcggt aatttgattg
    26701 aggacgaagg tttccgacga cagcgggaat ttcaataaga agaagccgac cttcatttca
    26761 cctccccgat gcgctcaagc acagactgca ccatctgcat tcccgtctga cgttcgcgac
    26821 tgacggcttc gttaaatcga gcgttcagca ccggaagctg gcctaaggta tccgcaacca
    26881 tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat cgccatctct ggcagtccca
    26941 gctgctgcat aatcccggcg gatttatgtt catagttgat ggcaatcgcc ggagtagcaa
    27001 agttcattga gataatggcg gagtgcaggc gcgtaccgac ggtgagttca caggctccga
    27061 gaattttgcc catttccaga tcgttgagtt catccatcac tacgtggtaa cgggcaggat
    27121 cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc gtctttgtta tagctgtcga
    27181 tacccgtaca ggtggagagc gcaatcacct gatagccttc atcgagaatg cgattgacca
    27241 ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc gagacgtttg tcgaacggtg
    27301 ccagttcgcg cagggtaatt gccactgttt tctgttgtgc ggcaacgtcc agccagtgtt
    27361 gaacggcata gctggcggtg aagtcttcgg tgtggtgatc gaccagccac gcggtatcga
    27421 cgccatgttc cacttttgcg gtggtgatat ttctgcgttt catcaaatca aggctgaccg
    27481 attcgcgcag gatcagcgcg tcgcagtgac caaagacata gttcgccagt tggttaaatt
    27541 gctcatcctg gaaggggccg acgctgtgac caatcataaa cagcggcttt ttcgccataa
    27601 atgtgcaaag cgcatgttca aactgcggca cgccgtagag atcgacaaaa aacgatccgc
    27661 cgacctgaat aatggcgtcg taacctgaca gcaggcgcac gaagtcggtg aatccctggg
    27721 cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg tgagagcaat acctgatgct
    27781 ggtagcggcg acggaggact tttttaatgc gcccgacaac gcccgccgcg ctattgtgtt
    27841 gtgtcatctg caggaacagc ggatcgccca ttaccgggcg gttgagcagc caggaagaac
    27901 tgaccggata gcggctcatc acgtccactt cggtatgtgg attgaggatg ttgatggcat
    27961 caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc gcaagtgtgg ttgcccagaa
    28021 taagtaattt cattttttcc tcataaattt gatgccaggt gaggctgtgt ttgttgtgtt
    28081 cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta tcaggcctac aggtcccgag
    28141 cacagaaccg taggacggat aaggcgtttt acgccgcatc cggcaaccgt tgtcggaacc
    28201 gaaaacagca attcaccccg cccgtaaaag cattttcatt ttttcgctgc gacaaaactg
    28261 acgcttcact tccaccacca gcggatggcg tgacagcaca atcatcacca caaacgccag
    28321 cacgcccgcg gctatttgca ccgccagcag catccccagc gccagttgcc ctttcagcac
    28381 aatgcccagc gcataactga ccaccagcgt cggcaacgag agataaaacg gcagccacaa
    28441 actcaggatg tactggcgat aactggaacc aagaaccggt ttaatcatca cgaagtaact
    28501 cagaatggtg ttgataatct gcaccagcag gaaaccgagc gtgacgccga ttgcgcccgc
    28561 catctgccca ccaataacaa tcgccggaat aaacagaaat gttttgaata cgttgaattt
    28621 aaagctgata tcgacccgcg ctttcgccat cagcagcgaa ccaatcgggt tacccacgga
    28681 gcgcagcaga cccacaatac acagcaattg cagcaccgga ataatgctgt tccacttttc
    28741 gccgaatacc agcggtacga agttattcga caccaccatc agccccagca gcgccggaaa
    28801 gttgataatc cccactatcg acagcagctt gtagaagtta acacgcagct tttcggtgtc
    28861 gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg atgattgggt tcagcttcat
    28921 cggcggtacg acggccacgt tgtacgccag gttgtatccc cctgccacgc ccgcgccaag
    28981 aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga tagttgatga tgctgtccgc
    29041 cgtcagccag gcaccaaaac gtaagttcgg tgccacagac gccagcgaga agtgcagacc
    29101 ggggcaataa attttgcgtc caaagtagcc aaacagcagt gttctcaccg cactattgac
    29161 cagataaccg aggatcgcgg tcatcgccag cggccagaaa tgggcgctaa ctaccgtaaa
    29221 agtgaagccc gccagcaccg cgctggtttc gatcatgccg attttgttga actccagctc
    29281 cttctgcatc aacgcgcgga actgttgccc gtgggggatc accacaaacg ccagcgacaa
    29341 tgttttaatc aacggtgcca ggtccgggtt attcagcacg tcgccgatga catcactcaa
    29401 caaaaacacc gccacacata ccacaagccc cagcccgacg ttcagccagt acagcgtggt
    29461 gagttcaagg tgactgattt cttttcgctg aataatcgag ttagcgatac cgaagtcaga
    29521 aagcgtatct gccagcgcga taatcaccag cgacacagtc agcaggccga actggtggtt
    29581 gtcgataatc cgcgccagca cggtcatctg caccagcccg aggccgatga tgatcaccgt
    29641 ggcaatagcc gaccacttcg cgccgctgat ggttttttca cgtaagctca tatcaatatg
    29701 ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac gattttgata tcgaaccaga
    29761 cgctccattc gcggatgtac tcaaggtcga actcgacgcg tttttccatt ttctccagcg
    29821 tgtcggtttc gccgcgccag ccattaatct gcgcccagcc ggtaatgccc ggtttcactt
    29881 tatggcgcag catgtagcct tcaatgagct ggcgatactg ttcgttatgc gctactgcgt
    29941 gcggacgtgg accgacaatc gacatccccc cggtcagcac attgataaac tgcggcaatt
    30001 cgtccagcga ggtgcggcgc agaaagttcc ccactttagt gacgcgcgga tcgttctgcg
    30061 tcgcctgggt caccactttg tcgttctcca tcactttcat ggaacggaac ttccacactt
    30121 tgatcggctt gccatccata ccatagcgag tctggcggaa aataaccggc cctggtgaac
    30181 tgagtttcac cgccagcgca atacagcaca gcaccgggga gatcagcagc agaataagcg
    30241 tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt aacccccgaa agcggcgtgt
    30301 catacagcgg caccaccgga acgccgttca tctcttcgag acgtgaatgg agaatgttga
    30361 aggtaaagac gtcggggatc agcagcaccg aacaggtggt atccgccagt tgatggacca
    30421 gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat atagacgtta tgaatcttgc
    30481 ccgcttttgc atcctcgacc agctgttgca ggttacccgc ccagtcgtta gaaacgccgc
    30541 ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc taaccacggc tgattacgga
    30601 aactctccat cagcatttgt ccggcggcta aatcccccgc tacggcgacc atccgcttgt
    30661 tgtagccatg attacgcagc cagcccgccc caatgcgaat acatgaacgg caaaccacca
    30721 gtccgatgct ggtcagccca taccacgcca gccagatttt cagttgcgtg tcgaaatcat
    30781 tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa ggtccagttt tgtagcaaca
    30841 gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg ataaaaatcg gtgatgccgc
    30901 ccagcatctg gaacaccacc agcgtaatca gcgccaccaa caggtgcatg tagaggaatg
    30961 acagtccgct gacttcgcaa accagccata gcccggcaaa catgatggtg atatctgaaa
    31021 agcgttgcac catagagatt aacgatgcat tggttttcgc tcgctcgcgc ttttttagat
    31081 ttgtcatcgt tgttcctgtt attagcccct tacccgcagt ggggtaaggg aagatccgac
    31141 attactcgtt cagcaacgtc agcagagttc gcgttcgcgc ttccatcagc ggcacatcac
    31201 cgcgcgattc cacattcagg cgcaccaccg gttcggtatt ggaggtgcgc aggttaaagc
    31261 gccagtcggc aaaggtcatg ctgatgccat cgctgcgatc caccgccagc gcctcacggc
    31321 taaaatgctg ttccacgcgg ttaatcgcct caacgggttg cgccagtttg ctgttgatct
    31381 caccgcttgc cggaaacgcc gccatccggt cgcgcaccag ttcgcccagc gtttttcctt
    31441 tcaggcacac cagttcggcg accagcagcc acgggatcat gccgctgtcg cagtaagcga
    31501 aatcacggaa gtaatggtgg gcgctcattt cgccaccgta gatggcgtct tccttgcgca
    31561 tccgttcttt aataaaggcg tgtccggttt tcgacattac cggcgtgccg cctgcagcag
    31621 tcaccacatc aatagtgttc caagagagac gcggatcatg gatgatcttc gcgccgggat
    31681 ttttttcgag gaacgcttct gccagcaggc cgacaatgta gtagccctcg ataaactgcc
    31741 ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc aaaggcaatg cccatatccg
    31801 cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca ttccggcagc agcgggttag
    31861 gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac tttgattaat tccacgggcg
    31921 cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac cggacccgct gcgccattcc
    31981 cggagttgat caccagcttg agcggcgtga gatttttgac attgatataa ccgaacaggt
    32041 gatcaacgta agcgtcacgc aggttgattt gctgatagcg accgcgtttg gtttcatcga
    32101 cgggaggaaa gtcgttggct tcagccagac gctggacgtc gcgcagtccg gtatcaccgc
    32161 tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt gtaatccatc ggattatggc
    32221 tggcagtaac ttcaatgccg ccatccacgc cgagatggaa cgtggcgaaa tagatctctt
    32281 cggtgccgga cataccgata tccagcacat cgacgcccgc atcctgtaaa ccttttgcca
    32341 gcgccagttt taaggtttcg ctggtgagac ggacatcacc gcctaacaca atggttttcg
    32401 gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc gatatcttca ttcagttctt
    32461 cgcctaattt cccgcgaata tcataggctt taaagcaggt taattttttc atgtcgttac
    32521 cctttttcag gcaatagttg gccctgaccg aaacggccaa ttttattttt gtcgttattt
    32581 acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc gcgtcttatc aggcctacaa
    32641 aaccgaaccg taggtcggat aaggcgttca cgccgcatcc gacaaacagc gcctgatgcg
    32701 acgtttacac ccgtccgtag cgatccgcga agcgcaccac atcatcctct tcgagataag
    32761 agccggagcg cacttcaatt aaatcgagcg gaattttccc cgggttttcc aggcagtgcg
    32821 tcgcccccag cggaatataa atggactcgt tttcaccaag cagtttgata tcaccatcaa
    32881 tggtgacttt tgccgttccc gcgaccacta cccagtgttc cgcgcggtga tggtgcatct
    32941 gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt cacctggtag cggtcgcccg
    33001 cgtcgataga gtcatatttg ccccacggac gatacacttc gcgatgcacc cgatgctcat
    33061 ggcgaccatc ggctttgatc tgctcgacca cttttttcac atcctgtacc gcgttacggt
    33121 cggcaatcag caccgcatct ttggtctgca ccactaccag atctttcacc ccgacggtgg
    33181 tgaccaggcc tgattcggca tacacatagc tgttttcagt tttgtgatta atcacatcgc
    33241 cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc ccataatgaa gaccaggaac
    33301 caacatcgct ccagcccgca tccatcggca ccacaacagc atctgccgta ggttccatga
    33361 ccgcgtaatc caccgactct tccgggcagg cgagaaacgc ttcttcatcc acacgaataa
    33421 aatcgagatc cggatcgacg gcgctcatcg ctttttcgca ggcatcgagg atatccgggc
    33481 gatatttttt cagttcttcg agatagcgtc cggcgcggaa caggaacatg ccgctgttcc
    33541 agtaatattc gccgcttgct acataggcct gggcggtttc cagattcggt ttttcgacaa
    33601 actgcgccac ttcaaaggcc accgtatcct gctcacccac agacacttca ccgcgacgaa
    33661 tatagccata accggtttct ggtagatccg gcacaatgcc aaaggtcacc agcttgcccg
    33721 cttcggcata aggcatggca ttacgcacgg cggcacggaa cgcgtcttca tcggcaatca
    33781 catgatccgc cgccaatacc agcattaacg ggtcgctctc cgggctatga cgttttgccg
    33841 ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc tgccggttcg agaataatgt
    33901 tctcggtgag tttgttcagt tgacgcagct gttccgcgac aataaagcgg tgctgctcat
    33961 tgcaaatcac caccgggctt tcgcactcca cgccgttcag gcggcagatg gtggtttgca
    34021 gcatggtgag atcgcctttc aggcataaaa actgcttggg gtaaagtacg cgggaaagcg
    34081 gccataaacg gctaccggag ccacctgcca tcacaactgg atagagtttc gactgcgcca
    34141 taattatccc cgaatatcat ttataaattg acgtaacacg ttctctttat cgagcgtgcg
    34201 ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc aggagcgcct gacgaatccc
    34261 cgccaccagc gcctcgaccg attccggttc tacgcaaacc gcaatacccg gaaaggtttc
    34321 gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc accgcgttac cgcctaccgc
    34381 cagaatattg gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt tttgcaccac
    34441 cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg tcatacgatt gcagcgggaa
    34501 aaattgcatg ttgcgcagtc cacgctgctg cgccattttt tccagccgcg ctttgccgcc
    34561 gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc aggagatcgg cagcttcaat
    34621 aacgttttcc agcccctgct tttcaccaat attgccggag taaagaatga tttttttgtt
    34681 atccggcagg ccaagctggt tacgaagggc atcaacatcg gcgtctgcaa catgctgaaa
    34741 acgggcaatt tccgaccagt tggggaagaa gatgacgtta tccgccgcca cgcctttttc
    34801 gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg ttatcgacgt tatgcagtcc
    34861 gctacgttcg aacgccgttg ccagctgtgc cactttgccg cctttgcctt ttccggcaag
    34921 gcccagcccc agcatggcgt ccacttcgta atcctgaata tgcagcacgg tacgcgcacc
    34981 agagagcttc gccagcaggc gcattcccgg cgtgcaaaac agcgttggca ctacgccaat
    35041 aatgcgatcc ggcttccagc gacgttgcgc catcagtgga aagaaactgc tgacggcaaa
    35101 actgccgaga tgcaacaagc gtttcagggt gctcggctgt tttggcacgt acagcgggca
    35161 gcgccacacc gtggcggccc cttcttctcg tttgtagcgc caggcggaat agttctcacc
    35221 cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc cgcacctcat gaccttgtgc
    35281 cgccaaccat tccaccatct cgccggtgta tttgccgatg ccggttaact ccggcgagta
    35341 gttaatgccg tagaccagta ttttcataat ccgggtactc cggcacgctt ctcagcgaga
    35401 aaataggctc ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc cggcgtcagc
    35461 cagcggtaat cgtcatgctg ctcatccggc agtaacagct cttcttccgc tactctgaag
    35521 cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc cggagaagtt atcgtcataa
    35581 aaatgctgcc agacaccgta aaactggcct gctgttatcg gcagacgcag ccccagttcc
    35641 gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt ctttctgcac gcgccctccc
    35701 ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt tgccaagcag aaactcgccg
    35761 cgactgttct cgacaataaa gtcgagagag acaagcggtg tggagcgcac tacagtggca
    35821 aagtcttcct gacgtaaaaa catcattccc cccgaaagcg gtcttgattc tcaaggaacc
    35881 actggtaagt gctggcaagc cccgcttcca gtgagatttc gtgataccag ccaagctgat
    35941 gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc cggtttgctg gcatcaaaaa
    36001 ccacccgacc tttgtaaccc accactttgg cgatggtttg cgccagctcg cggatagtgc
    36061 agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg ttgggtattc tccagccaga
    36121 cttcatgcgc cagctccatg acatgaatgc tcgccgccgc catatcatcg acgtgcagga
    36181 attcacgcat cggtgtaccg ctgccccata ccaccacgtc cggcgcattc tgtgccgtcg
    36241 cctcgtggaa gcgacgcagc aatgctggta tcacatggga gttactcggg tggaagttgt
    36301 catgcggccc gtacaggttg gtcggcatga cagtgcggta atcgcgtcca tactggcggt
    36361 tgtaagattc gcacagtttg atcccggcga ttttggcaat ggcgtaaggc tcgttggtcg
    36421 gctccagcgt gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc agtttcgggt
    36481 agatgcagga cgatccgaga aacatcagtt tgttcacgtc gttctgatgc gcggcgtgaa
    36541 tgatgttgct ctcaatcatc atgttctggt agatgaaatc cgccggatag gtgttgttgg
    36601 caacaatgcc gcccactttc gccgccgcca gatagacctg gtcaatgcgt tcgctggcaa
    36661 agaaatcatg cacggcgcgg ctgtccagca ggttcagttc atcgcgggta cgtaatacca
    36721 gctccacatc accgcgctgt tcgagctgcc gcttgatggc agaaccgacc atcccacaat
    36781 ggccagcgat aaaaatgcgt tgtttactca tgcttatgac tccagcgcga tcgccacgtc
    36841 gtagccgtga gatttcagca gagagtgttt tttcgccgct tcgaggtcat tagccaccat
    36901 ttcagacacc atctctctga gggtgatttc cggtttccag cccagttttt cgtgcgcttt
    36961 ggtcgggtcg ccgagcagcg tttcaacttc tgccggacgg aagtaacgcg gatcaacggc
    37021 gataatcaca tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg aaaccacaat
    37081 gcccttctct tcaacacccg tgccttcaaa gcgcagtttg atgcccagct gtgctgccgc
    37141 catttccaca aactgacgta cggagtactg aacgccggtc gcgataacga aatcttccgg
    37201 ctgttcctgt tgcagcatca tccactgcat ttttacgtag tctttggcgt ggccccagtc
    37261 acgcagggaa tccatattgc cgaggtacag gcacgactcc agcccctggg cgatgttggc
    37321 gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg attcatggtt
    37381 gaagagaatt ccgttacaag cgtacatgcc gtaggattcg cggtagttaa cggtgatcca
    37441 gtaggcgtac agtttggcga ccgcatacgg agatcgcggg tagaacggcg tggtttcttt
    37501 ctgcggaatt tcctgcacca gaccatacag ttcagaggtg gaagcctgat agaaacgagt
    37561 tttcttttcc agaccgagga agcggatcgc ctccagcagg cgcagcgtac ccatcgcgtc
    37621 gacgtcagcg gtatattctg gtgactcaaa agagaccgca acgtggctca ttgcgcctag
    37681 gttgtacact tcatccggct gtacttcacg caaaatgcgc gtcaggttag aggtatcact
    37741 caggtcgcca taatgcagat ggaatttcgg gttgcaggtg tgcggatcct gataaatgtg
    37801 atccacgcgc tcggtgttga atgacgatgc gcgacgctta ataccatgca cctcgtaacc
    37861 tttttccagc agaaactctg ccaggtaaga accgtcttgt ccggttacac cggtgatgag
    37921 agcgactttt gacatgtctt attcctctgt atttttgaat ttattcagtt tcaacgcgtt
    37981 cgcgtatcac cactgcggga ttcccccggc aaaccacatt tgccggaagc gatttaaaaa
    38041 cactgcttcg tgcacccacg acggtgccgt cgccgattgt gacgcctggg gcaacaaaga
    38101 catcggttgc cagccagcat ttctcgccaa tcacaatagg cgtggcgtta atggtgaaat
    38161 gttgacttgc atggtcgtgg ctaccggtgc ataaataact tttttgcgat atcaccgaat
    38221 gtgcgccaat ggttatttca ccgagggtat ataaattgac gtcatcgcca acccacgcgt
    38281 aatcacctaa ggttaatttc cacg
//
LOCUS       DAJTYM010000030        37342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTYM010000030 DAJTYM010000000
VERSION     DAJTYM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..868)
                     /gene="yhdJ"
                     /locus_tag="OO575_001552"
     CDS             complement(<1..868)
                     /gene="yhdJ"
                     /locus_tag="OO575_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6664560.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDP"
     gene            complement(954..1250)
                     /gene="fis"
                     /locus_tag="OO575_001553"
     CDS             complement(954..1250)
                     /gene="fis"
                     /locus_tag="OO575_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6664561.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1276..2241)
                     /gene="dusB"
                     /locus_tag="OO575_001554"
     CDS             complement(1276..2241)
                     /gene="dusB"
                     /locus_tag="OO575_001554"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6664562.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2570..3451)
                     /gene="prmA"
                     /locus_tag="OO575_001555"
     CDS             complement(2570..3451)
                     /gene="prmA"
                     /locus_tag="OO575_001555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6664563.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3463..4914)
                     /gene="panF"
                     /locus_tag="OO575_001556"
     CDS             complement(3463..4914)
                     /gene="panF"
                     /locus_tag="OO575_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6664564.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4904..5146)
                     /locus_tag="OO575_001557"
     CDS             complement(4904..5146)
                     /locus_tag="OO575_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6664565.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5255..6604)
                     /gene="accC"
                     /locus_tag="OO575_001558"
     CDS             complement(5255..6604)
                     /gene="accC"
                     /locus_tag="OO575_001558"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6664566.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6615..7085)
                     /gene="accB"
                     /locus_tag="OO575_001559"
                     /gene_synonym="fabE"
     CDS             complement(6615..7085)
                     /gene="accB"
                     /locus_tag="OO575_001559"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6664567.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8063..9037)
                     /locus_tag="OO575_001560"
     CDS             complement(8063..9037)
                     /locus_tag="OO575_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6664568.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9189..11129
                     /gene="csrD"
                     /locus_tag="OO575_001561"
     CDS             9189..11129
                     /gene="csrD"
                     /locus_tag="OO575_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6664569.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11434..12477
                     /gene="mreB"
                     /locus_tag="OO575_001562"
     CDS             11434..12477
                     /gene="mreB"
                     /locus_tag="OO575_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6664570.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12543..13646
                     /gene="mreC"
                     /locus_tag="OO575_001563"
     CDS             12543..13646
                     /gene="mreC"
                     /locus_tag="OO575_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6664571.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13646..14134
                     /gene="mreD"
                     /locus_tag="OO575_001564"
     CDS             13646..14134
                     /gene="mreD"
                     /locus_tag="OO575_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6664572.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14143..14736
                     /gene="yhdE"
                     /locus_tag="OO575_001565"
     CDS             14143..14736
                     /gene="yhdE"
                     /locus_tag="OO575_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6664573.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14726..16195
                     /gene="rng"
                     /locus_tag="OO575_001566"
     CDS             14726..16195
                     /gene="rng"
                     /locus_tag="OO575_001566"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6664574.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16263..20063
                     /gene="yhdP"
                     /locus_tag="OO575_001567"
     CDS             16263..20063
                     /gene="yhdP"
                     /locus_tag="OO575_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6664575.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20219..21664
                     /gene="tldD"
                     /locus_tag="OO575_001568"
     CDS             20219..21664
                     /gene="tldD"
                     /locus_tag="OO575_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6664576.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21792..22721)
                     /gene="aaeR"
                     /locus_tag="OO575_001569"
     CDS             complement(21792..22721)
                     /gene="aaeR"
                     /locus_tag="OO575_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6664577.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22904..23107
                     /locus_tag="OO575_001570"
     CDS             22904..23107
                     /locus_tag="OO575_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6664578.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23115..24047
                     /gene="aaeA"
                     /locus_tag="OO575_001571"
     CDS             23115..24047
                     /gene="aaeA"
                     /locus_tag="OO575_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6664579.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24053..26020
                     /gene="aaeB"
                     /locus_tag="OO575_001572"
     CDS             24053..26020
                     /gene="aaeB"
                     /locus_tag="OO575_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6664580.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26112..26384
                     /locus_tag="OO575_001573"
     CDS             26112..26384
                     /locus_tag="OO575_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6664581.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26440..26703)
                     /gene="yhcN"
                     /locus_tag="OO575_001574"
     CDS             complement(26440..26703)
                     /gene="yhcN"
                     /locus_tag="OO575_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6664582.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27068..27538)
                     /gene="argR"
                     /locus_tag="OO575_001575"
     CDS             complement(27068..27538)
                     /gene="argR"
                     /locus_tag="OO575_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6664583.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            27973..28911
                     /gene="mdh"
                     /locus_tag="OO575_001576"
     CDS             27973..28911
                     /gene="mdh"
                     /locus_tag="OO575_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6664584.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(28973..30040)
                     /gene="degS"
                     /locus_tag="OO575_001577"
     CDS             complement(28973..30040)
                     /gene="degS"
                     /locus_tag="OO575_001577"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6664585.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30130..31477)
                     /gene="degQ"
                     /locus_tag="OO575_001578"
                     /pseudo
     CDS             complement(30130..31477)
                     /gene="degQ"
                     /locus_tag="OO575_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31631..32029)
                     /locus_tag="OO575_001579"
     CDS             complement(31631..32029)
                     /locus_tag="OO575_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6664586.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32223..33350
                     /gene="zapE"
                     /locus_tag="OO575_001580"
     CDS             32223..33350
                     /gene="zapE"
                     /locus_tag="OO575_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6664587.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33569..33997
                     /gene="rplM"
                     /locus_tag="OO575_001581"
     CDS             33569..33997
                     /gene="rplM"
                     /locus_tag="OO575_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6664588.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34013..34405
                     /gene="rpsI"
                     /locus_tag="OO575_001582"
     CDS             34013..34405
                     /gene="rpsI"
                     /locus_tag="OO575_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6664589.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34800..35438
                     /gene="sspA"
                     /locus_tag="OO575_001583"
     CDS             34800..35438
                     /gene="sspA"
                     /locus_tag="OO575_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6664590.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35444..35941
                     /gene="sspB"
                     /locus_tag="OO575_001584"
     CDS             35444..35941
                     /gene="sspB"
                     /locus_tag="OO575_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6664591.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(35984..37342)
                     /gene="dcuC"
                     /locus_tag="OO575_001585"
     CDS             complement(35984..37342)
                     /gene="dcuC"
                     /locus_tag="OO575_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6664592.1"
                     /translation="MIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGV
                     LDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSLLSR
                     PLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVI
                     ATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDK
                     KDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVVMLL
                     SLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVD
                     AVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVV
                     MIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITL
                     FY"
BASE COUNT         9023 a         9354 c         9848 g         9117 t
ORIGIN      
        1 cggggtcaac ttcgcttaac cggcttcgtt ttgacaggtt gccggtcttt ctttttttca
       61 ctttcgccag ttcttccgcg gagtaatgcg acgcgacatc cagccgtcga agccccattt
      121 tgatgtactc gctgttgatc tcaataccaa tgaattttcg tccgctggcg atggctacgg
      181 caccggtagt aaagctaccg gcaaacgggt cgagaacgat atcgcctggg ttggaagagg
      241 cgagaataat gcgtttcagt aaggctttcg gtttttgcgt cgggtggttc tcatattcat
      301 ccatcaaata acgcacgcgc ggaaaatccc agacgttacc cggtactttt tgatgattgt
      361 atggctgtgg aggatttttg cgataatcga tcaacgcgcg ctgcgatccg gttttggctt
      421 ctaccagaat agcatcaccg ttgaatgtgt agttctttgc gtctttcacc atcatcagga
      481 tgggttcgta catggagccg tagtgttttt tcgcctgtac tccagaactg tcatatgacc
      541 agacgatgcg acttttgatg gtaaaaagct tgcggcactg gagatcgata aagggcatgt
      601 tttccgtact gttcataatg tacatgctgc cctgcttttt cagaacgcgg tggcactctg
      661 caatcacttc aaacagccag tcgataaaca gatcttcttt ccaggcttcg atcagaccat
      721 caaaattttt accgatgtta tacggtgggt cggcaaagag cagatcgacg ctttcagtag
      781 gtagcttttt aagttcggca aaggcatcac cgtgaataat ggtcttagct tcattaccaa
      841 accgggtcgg ttcacatcct gttctcatgg tcactccctt tgtgacacct ataaaaaagg
      901 cgcttcccca tgccgagtag cgccttttta atcaagcatt tagctaacct gaattagttc
      961 atgccgtatt ttttcaattt tttacgcagc gtaccacggt tgatgcccat catcagcgca
     1021 gcacgggtct ggttaccacg ggtgtattgc atcaccatgt ccaacagggg ctgttctact
     1081 tcagccagta ccagctcata gaggtcattc acatcctgac cattcagttg agcaaaatag
     1141 ttcttcagtg cctgtttaac cgagtcacgc aggggttttt gggttacctg atcctgagag
     1201 ttaacggtag aaacggtcag tacgtcagaa tttacgcgtt gttcgaacat agttctgtca
     1261 gctctttatt tctgtttacg caaaattttc gaagtatgcc tccaacgcct ccagctgttc
     1321 gctggcatcc tcaatggcgt tgaatgtgcg ccgaaactgg tcatttggag cgtgttcctg
     1381 gagataccag gaaacgtgtt tacgtgcaat tcggtaccct tttgccggac cataaaagtc
     1441 atgcagttcc cgaacgtgcg cgcaaagcaa gcgcttaacc tctgccaaag gcagtggggg
     1501 cagcaactcc ccagtgtcca gataatgctg gatttcccga aagatccagg gtcttccctg
     1561 agctgcgcgg cctatcatca gggcatccgc ccctgtatag tcgagcacag ctctggcttt
     1621 aagcgggtca gtaatgtcgc cattcgcgat aaccggaatg gaaactttct gcttaactgc
     1681 ccgaatactg tcgtactcag cttctccatt gaacaaacag gcgcgtgtac ggccatgaat
     1741 ggtcagagcc tgaatgccac agtcttcagc cagttgggca atctcttcgc agttacggtg
     1801 ttccggtgcc cagccggtgc gaatcttcag ggtaacagga acgtccactg cattgacgac
     1861 ctcggtaagg atcgatttaa cgacatccgg gtactgcaag agggctgaac ctgcgagctt
     1921 gcgattcact tttttagccg ggcaacccat attgatatca ataatctggg caccgctttc
     1981 cacgttaata cgtgctgcat ctgccatttc tttcggagcg ctaccagcaa tttgcacggt
     2041 gcgaataccg ggttcatcaa tgtgcaccat ccgtaaacga gatttgtcgc tttcccaaac
     2101 ctgtgggtta gaagacatca tctcggatac tgtcaatccg gctcccatct cgtagcacaa
     2161 cgtccgaaaa ggtctgtctg taatgccagc catgggcgct gcgatcaggc gatttctgag
     2221 ctgatattgt ccgatgcgca tgagttaaga aatgaccata ctgtgactgc aaggcggcgt
     2281 atattacgca ttttttgcac gagatgaaag gccaaacttt gaccaatcct ctgagatgga
     2341 tcaaagaatt gcatttaaaa tgagcgtggt gcgataatta ctcataaaaa tcatcatatt
     2401 agaaaatagt gactaaaatt tacactcaaa gaaatttgag taagttctca atttttcttt
     2461 atgaatgaaa attttggcac gcaaatttgc gtaaataatc ggcaaattta cgtgcctttt
     2521 gtgagcttgc tcgcacttcg ccccgcgtca ccctacggcg atgcgaaggt caattcttac
     2581 gaccggtaat acggcaccac tcttcttttt ccacgaccgg gtccagtgcg aagctatcgg
     2641 cataagcttc acaaacgctc tctgcctggc ttgccagaat accggaaagg cccagcaaac
     2701 cgcctgaaac tggcaggacg ctgattaacg gtgccagttc acgtaatggg cctgcaagga
     2761 tgttagcgac caccacgtcg gctttcattt cttctggctg atctttcggt aagtagagtt
     2821 ccagacggtc agaaacgcca ttacgttcgg cgttatcgcg gctggcctga atcgcctgcg
     2881 gatcgatatc aataccaatg gcttttgctg cacccagttt cagcgccgcg atcgccagaa
     2941 tgccggaacc acagccaaag tcgatgactg ttttaccggt taaatcgagg ctgtcgagcc
     3001 attgcaggca cagagaggtg gttggatggg tacccgtacc aaacgccagc cctggatcta
     3061 acatcacgtt gacggcgttt tcgtccggca catcacgcca gctagggcag atccacagtc
     3121 gttcaccaaa gcgcatcggg tggaaattat ccatccattc gcgctcccag tctttatctt
     3181 ctagttgttc gattttatgc gcgaagcctg cgccgagcag cggatggttt tccagaatcg
     3241 ccaccacgtc gttcatatcg gtttcagcgt cgaacagacc aatcacatcg gtgtcgcccc
     3301 acaggcgcgt ttcgcccggc agcggctcaa atactggcgt atcgtgggta tcctgaaaag
     3361 tgatagaaac ggcacccgct tccatcagcg catcgctaag atcttccgcg ttcgcgccgg
     3421 tggtgttcag tttcagttgg atccaaggca tggcaaaact ctttatttat cagtagtcaa
     3481 aacggtagct tgcgggacgg atgtaccgaa acggtttccg accaggaaag ccagcaaact
     3541 tagtagtaac gagggcacga tagggtggaa gcccaggtac tgaatattca gcgtcgcgag
     3601 tacggcatac agcacgccgc caacgatcat cgcacttagc gcgcctttgg cgttggcgcg
     3661 ttcccagtaa agacccagca ccagcggcca caggaaaacg gcttccagcc caccgaaggc
     3721 cagcaaattc agccagatga tcatttctgg cggcttccag gcggcaagca gcagcaacgc
     3781 gccgagaact aacgtaatta ccgccgacat ccgcttcaga cgcgtctcgt tttgcatttg
     3841 atccggacgg atattcagat agagatcttt aatgatcgta gcggaacttt gcagcagttg
     3901 ggcgttaatt gtcgacatga tcgcagccat aggtgcagcc agaaagatcc cggcagcaaa
     3961 aggtggcagc acttttacca ttaacgtcgg gatcaccaga tctggtacgg tgagatcggg
     4021 gatcactgca cgacctaacg ctccggccag gtgcataccg aacatcagga ttgcgactat
     4081 aatcgtaccg atgatgatcc cccggtgtac tgctttgctg tctttataag agatacagcg
     4141 caccgcagta tgcggcaggc caatcacgcc aaagcacacc agtacccaga acgacgtcat
     4201 aaaggcaggc gacagaatat cgtcagcgcc ttgtggcgta accagttgcg gatcgatggt
     4261 ttgcaaggtc tgtactgcgt tgcttaagcc gccagcggca tgtaccacgc caataagcag
     4321 cacaacggtg ccaatcagca tcacaagccc ttgcatggtg tcgttcagca cgctggcgcg
     4381 aaagccacca aaggcggtat ataacgcaat gctgatacca aaaatcagca gcccggtttc
     4441 ataaggaata cccgccgcgg tttccagcag gcgcgcaccg ccgataaact gcacggtcat
     4501 cgcaccaacg aacgcaacca gcaaactcaa actcgccagc cacaccagaa gacgactctg
     4561 gtagcgggca aacagcatat cgttcagcgt cactgcattg tagcggcgcg caagaatcgc
     4621 aaacttcttg ccgagaatac cgagtgaaag ccagacagca ggaagctgaa tcatcgccag
     4681 caatacccag cccagcccgt atttataagc agctcctggc ccgccgataa acgaactggc
     4741 actgatatag gtcgcggtga gagtcatcgc cagcacaata ccgcccatag agcggctgcc
     4801 gaggaaatac tcattaagga aggtgccggt gctccgttta cgcatcgcat aaaccgagat
     4861 accgaacacc accaccagat aggcgaccag cggtagaatt acttcaagct gcatcgtcat
     4921 cctccagtgg gatatcgcga tagataaatt tcaccatcgc ccagcacagt ccaataaaca
     4981 gtagcggcgt caggatgcag gccatctcaa accagcgcgg aaagccggta aaaccggggg
     5041 caacgccagg taagtaagcg tctactaacc aaactgccag atacaaaagg gtcagcccca
     5101 gcgcccagcg ggcctcttta tgggcctgaa caaaacgagt gtccattttt tgtccctgat
     5161 gggtgaagaa agcgaggatt gtaccttatg ggggttgtcg atccccagta ataaaaaagg
     5221 ccggaaaatc cggccttttg acgcttttgc agtcttattt ttcctgaaga ccgagttttt
     5281 tctccagata gtggatgtta gtgccaccat gctggaagtt ctcgtcattc atgatgcgga
     5341 tctgcagatc aacgttggtt ttgataccgt cgatgatcag ctcctgcagc gcattcttca
     5401 tgcgggcaat cgccacgtca cggttttcac cgtagcaaat cagcttaccg atcattgagt
     5461 catagtacgg cggtacggtg tagcccgcgt agatatgaga ctcccaacgt acgccaaaac
     5521 cgccaggtgc gtggaaacgg gtgattttgc ctgggcttgg caggaaggtg ttcggatctt
     5581 cggcgttgat acgacattcc accgcatggc cgcgaacgtg aacttcttct tgcttgatcg
     5641 acagcggttg accggcagcg atacgcagct gttctttgat caggtcaacg ccggtgatca
     5701 tttctgtaac cgggtgttct acctgaatac gggtgttcat ttcgatgaaa tagaactcgc
     5761 cgttttcgaa caggaactcg aaagtacctg caccgcgata gccgatatca acacacgctt
     5821 tagcgcaacg ttcgccgatg tagcgacgca gttccggggt aatgcccggt gctggtgctt
     5881 cttcgaccac tttctggtgg cggcgctgca tggagcagtc acgttccgcc agatagatag
     5941 cgttgccctg accgtcagcc agtacctgaa tctcgacgtg gcgaggattc tccaggtatt
     6001 tctccatgta aaccatatcg ttgctgaaag cagctttcgc ttccgcacgg gtcatggaga
     6061 tggattgtgc cagttcagcg tcgccgcgca ctacgcgcat accgcgaccg ccgccgccgc
     6121 cggaggcttt gataatcacc ggataaccaa tgcgtttagc aatggcgcgg tttttatcca
     6181 tatcgtcgcc cagcgggccg tcagaaccag gtacgcaagg aacgcccgct tttttcatcg
     6241 ccgcgattgc ggatactttg tcgcccatca ggcgaatggt ttctgctttc gggccgatga
     6301 agataaagcc ggagcgttca acctgctcgg caaagttggc gttctcggag aggaagccgt
     6361 aacccggatg gattgctact gcgccggtga tttcagcggc gctgatgatt gccgggatgt
     6421 tcagataact ttttactgac ggagcagggc caatacagac cgtttcatct gccagtaata
     6481 cgtgttttag atcgcgatcc gtgctggagt gcacagcgac agtcttgatg cccagttctt
     6541 tacaggcacg aagaatacgc aatgcaatct cgccgcggtt ggcaataaca attttatcca
     6601 gcatgttcgc ctcgttactc gatgacgacc agcggctcgt caaattctac cggttgtcca
     6661 ctttcgacca gaattgcttt cacggtaccg gatttgtccg cttcgatctg gttcatcatt
     6721 ttcatggctt caacgatgca cagggtatcg cccacgttga ctttctgacc cacttcgatg
     6781 aacgcttttg cgtccgggct tggggtgcgg tagaaagtac caaccatcgg ggaacgtacg
     6841 atgtgaccac tgatttccgc tgctgctggc gcttccatgg aaggaacggt cgccggagcg
     6901 gctgcgttag attgagctgg ctgctgcatc attggtgcag cgtaagcttg ttgcatcaca
     6961 gggaaacttg cggcaggagc tgcacggcta atgcgtactg actcttcgcc ttcagaaatt
     7021 tccagttcgg agatgcctga ttcttcaacc agctcgatca gttttttaat cttacgaata
     7081 tccatgagtg ggttccgtac tctttgttta gtgtgattgt gacaggcgtt tcaccgccgt
     7141 ctgtaaagcg tatgccccga cagctgtatg catagcgata aattccagca ggccgggacg
     7201 ccggtctatt ttgccccaga gtgtcaatgt tagacttgac ggacattgtg cagcgatctg
     7261 ctgctatcct ccggcaaaaa acaaaatata ccttcgttgc gccaatgtca ccttttccgc
     7321 agcgcaaaaa ctgcgtcagg gaggacgagg tcgcacatta taacgatttc gtagcaattg
     7381 gcagctaaat actggtctta tcagggaata taatcaacag ctaacatgta aataaccttc
     7441 aacaccgtgt aatttgcaac aagccgcaca attcacgaaa ttagcgccat catcgacgga
     7501 acttcttata acgtaaggtg aaaagctgaa aagccagccc tgcgaagatg aggggctgca
     7561 atgagataat cttcacagac cacaaataat gtagggatgc caggatcgcg acaagataga
     7621 cgacattacg cagcggttgc tggggattac ccaattttca ctgcatcgtc tggatagatg
     7681 tgaatgctaa agcacgcaaa atcaaccaac tgacaattct cagcattaaa taggttcgag
     7741 tggttaattt gtagtcaaat aaaatcagat tattcacccc atggtaccag aaatcagtaa
     7801 caggttaagc gtagtgtcgc ccaggcaaag caccataaac ctaacaggcg acggaggcgt
     7861 atcaataatg gctgtttaga ggagtatcaa tgcgcatcaa tttgtggtca gtcgaaatca
     7921 tgactttcca gtttggatga tgttgtacaa cttcctgacc aaggtgttcg acgaaagctg
     7981 tccggctaac cgcttttccc gttaaccgat tgaataatga tgtcaaaatg agttccctct
     8041 cttattattc cttgctaaat ggttagttaa ccttcaccag cgtgcgaccc tggatctggt
     8101 tattaatgat ggcctcggca aagttcggtg cctctgacag agatatctct tttgccgcct
     8161 gggtatagaa tgattccggt aaatcggcga ccagtcgctg ccaggcttgt gcgcggcgtt
     8221 ctggtggcgt cattactgaa tccacccctt gcaaacggac attacgcaga ataaatggca
     8281 tgaccgtggt tggcagagta aaaccacccg ccagaccaca ggccgccacg cagccgccgt
     8341 aattcatttg cgccagcact tttgccagca ctttgtcgcc aacagtgtca attgccccag
     8401 cccagacttg tttttccaga ggacgggatt cggcaaactc atcacgaggg agaataccgc
     8461 tggcacctaa acttttcaga tattcatggg tactttcgcg accggaaacg gcaacgacct
     8521 gataacccaa cttatgcagc agcgccacgg cggtactgcc gacgccacca ctggcacccg
     8581 tcacgacaat ctcaccgtcc tgcgggcgaa caccggcatc ttccagcgcc atcacacaca
     8641 gcatggcggt aaaaccggca gtaccgataa tcattgcttt acgcgcgtcc agcccttgcg
     8701 gcatggcaac cagccagtca cctttcactc gcgcctgctc cgccagccca ccccagtggt
     8761 tttcaccaac gccccagcca gtgagtaaca cctcctgacc ggcatgaaaa cgcggatctt
     8821 cgctggtgcg tacagttccg gcaaaatcga tcccaggaat catcggaaaa ttacggatga
     8881 tttttccctt accggtaatc gccagcgcat ctttatagtt caggctcgac cagtgaacat
     8941 cgaccgtgac atcgccctcc ggcaggcgac gttcgtccag agtctgtact gatgcgagag
     9001 ttttgccgtc ctgctgttct aaaagtaacg cctgcataag tggtcctcat gtgcatgatg
     9061 gattggaaaa taatttctga agactatact cgctaatgga aaagcaaagc cgatgaagcg
     9121 caataaattg cgagataaat ctgatttgct agtatgcccg cttcctcact atcggagtta
     9181 acacaaggat gagattaacg acgaaatttt cggcctttgt tacgctgctc accgggttaa
     9241 caatttttgt gactttgctg ggctgttcgc taagtttcta caacgccatt cagtataagt
     9301 ttagtcatcg cgttcaggcg gtggcgacgg cgattgatac ccaccttgtg tcgaatgact
     9361 tcagcgcatt aaggccacaa attaccgaat taatggtgtc agcagatatc gttcgtgtag
     9421 acctgctgca tggtgataaa caggtttata ccctggccag aaatggtagt tatcgtccgg
     9481 ttggcaccag cgatctgttt cgtgaactga gcgttccgtt gataaagcat ccggggatgt
     9541 cgttgcgtct ggtttatcag gatccgatgg gcaactattt ccattcgttg atgaccaccg
     9601 cgccgctcac gggggcgatt ggctttatca ttcttatgct ctttctggcg gtacgctggt
     9661 tacaacggca acttgccggg caagaattgc tggaaacccg ggctactcgt atcttaaacg
     9721 gtgagcgtgg ctctaatgtg ttgggaacca tctatgaatg gccgcccaga accagcagtg
     9781 cgctggatac gctgcttcgt gaaattcaga acgcacgcga acaacacagc cgtcttgata
     9841 cgctgatccg ctcttatgcc gcacaggacg tgaaaaccgg cctcaataac cgactctttt
     9901 tcgataatca gttagcaacg ttactggaag atcaggagaa agtaggtacc cacgggatcg
     9961 tgatgatgat tcgtctgccg gatttcaata tgttgagcga tacctggggg cacagccagg
    10021 ttgaagaaca gttcttcact ctgacgaatc tgctgtcgac atttatgatg cgctaccctg
    10081 gcgcactgct ggcgcgttac caccgcagtg attttgctgc gctgttaccg caccggacgt
    10141 taaaagaggc agagagcata gccagtcagt taatcaaagc cgtcgatacc ttgccgaaca
    10201 ataaaatgct cgatcgcgac gatatgatcc acattggtat ctgtgcctgg cgtagtggtc
    10261 aggataccga gcaggtaatg gaacatgcag agtctgccac gcgtaatgcg ggattgcagg
    10321 gtggcaatag ctgggctatt tatgatgact cgttgcctga aaaaggacgc ggtaatgttc
    10381 gctggcgtac gcttatcgag caaatgctca gtcgcggcgg cccgcgcctt tatcaaaaac
    10441 cggcggttac tcgcgaaggt caggttcatc atcgcgaact catgtgccgc atcttcgatg
    10501 gtaatgaaga ggttagctcg gcggagtata tgccgatggt cttgcagttt ggcttatcgg
    10561 aagagtatga ccgtctgcaa atcagccgtc ttattccact attgcgttac tggccagagg
    10621 aaaatctggc gattcaggtt accgttgagt cgctgattcg tccgcgtttt cagcgttggc
    10681 tgcgcgatac gttaatgcaa tgtgaaaaat cacaacgaaa acgcataatt attgaacttg
    10741 cagaggccga tgtaggtcaa catatcagtc gtttacaacc tgttattcgt ttagtgaatg
    10801 ctttaggggt acgggtagcc gtcaaccagg ctggtttgac gctggtaagc accagttgga
    10861 tcaaagaact taatgttgag ttactcaagc tccatccggg gctggtcagg aacattgaga
    10921 agcgaacgga gaaccagctg ctggttcaaa gcctggtgga agcctgctcc gggaccagca
    10981 cccacgttta cgccaccggc gtgcgttcgc gaagcgagtg gcagaccctg attcagcgcg
    11041 gtgttacagg cgggcaaggg gattttttcg cgtcctcaca gccacttgat actaacgtga
    11101 aaaaatattc acaaagatac tcggtttaac ctgccgttta atccgttttc acgtagaata
    11161 acgcgcgctg cgtctcatgg gagtgtgctt gtctgctcgc cagattgttg cagcacatat
    11221 gcagatgaat gaccttacgc ggttgcaaac aggcgaggaa tgctgctgat gcattaagcc
    11281 tttctggact caggcagaga tttgtaacaa aggaaacgaa ctgcactaat tttcaccgta
    11341 gcagatgatt tttgcgcctt gtcgctgctg cgtgtggttg gtaaagtaag cggattttct
    11401 tttccgcccc agctttcagg attatccctt agtatgttga aaaaatttcg tggcatgttt
    11461 tccaatgact tgtccattga cctgggtact gcgaataccc tcatttatgt aaaaggacaa
    11521 ggcatcgtat tgaatgagcc ttccgtggtg gccattcgtc aggatcgtgc cggttcaccg
    11581 aaaagcgtag ctgcagtagg tcatgacgcg aagcagatgc tgggccgtac gccgggcaat
    11641 attgctgcca ttcgcccaat gaaagacggc gttatcgccg acttcttcgt gactgaaaaa
    11701 atgctccagc acttcatcaa acaagtgcac agcaacagct ttatgcgtcc aagcccgcgc
    11761 gttctggttt gtgtaccggt tggcgcgacc caggttgaac gccgcgcaat tcgtgaatcc
    11821 gcgcagggcg ctggtgcccg tgaagtcttc ctgattgaag aaccgatggc tgccgcaatt
    11881 ggtgctggcc tgccggtttc tgaagcgacc ggttctatgg tggttgatat cggtggtggt
    11941 accactgaag ttgctgttat ctccttgaac ggcgtggttt actcctcttc cgtgcgcatt
    12001 ggtggtgatc gtttcgacga agctatcatc aactatgtgc gtcgtaatta cggttctctg
    12061 atcggtgaag ccaccgcaga acgtatcaag cacgaaatcg gttcggctta cccgggcgat
    12121 gaagtccgtg aaatcgaagt tcgtggccgt aacctggcag aaggtgttcc acgcggtttt
    12181 accctgaact ccaacgaaat cctcgaagca ctgcaggaac cgctgaccgg tattgtgagc
    12241 gcggtaatgg ttgcactgga acagtgcccg ccggaactgg cttccgacat ctccgagcgc
    12301 ggcatggtgc tcaccggtgg tggcgcactg ctgcgtaacc ttgaccgttt gttaatggaa
    12361 gaaaccggca ttccagtcgt tgttgctgaa gacccgctga cctgtgtggc gcgcggtggc
    12421 ggcaaagcgc tggaaatgat cgacatgcac ggcggcgacc tgttcagcga agagtaatcg
    12481 gatgcaggca ggggaagcgt ctgtttaccc tgcctggtct gatacgagaa tacgcataac
    12541 ttatgaagcc aatttttagc cgtggcccgt cgctacagat tcgccttatt ctggcggtgc
    12601 tggtggcgct cggcattatt attgccgaca gccgcctggg gacgttcagt caaatccgta
    12661 cttatatgga taccgccgtc agtcctttct actttgtttc caatgctcct cgtgaattgc
    12721 tggatggcat atcgcagacg ctggcctcgc gtgaccaact agaacttgaa aaccgggcgt
    12781 tacgtcagga actgttgctg aaaaacagtg aactgctgat gcttggacaa tacaaacagg
    12841 agaacgcgcg tctgcgcgag ctgctgggtt ccccgctgcg tcaggatgag cagaaaatgg
    12901 tgactcaggt tatctccacg gttaacgatc cttatagcga tcaagttgtt atcgataaag
    12961 gtagcgttaa tggcgtttat gaaggccagc cggtcatcag cgacaaaggt gttgttggtc
    13021 aggtggtggc cgtcgctaaa ctgaccagtc gcgtgctgct gatttgtgat gcgacccacg
    13081 cgctgccaat ccaggtgctg cgcaacgata tccgcgtaat tgcagccggt aacggttgta
    13141 cggatgattt gcagcttgag catctgccag cgaatacgga tattcgtgtt ggtgatgtgc
    13201 tggtgacctc cggtctgggc ggtcgtttcc cggaaggcta tccggtcgcg gttgtctctt
    13261 ccgtaaaact cgatacccag cgcgcttata ctgtgatcca ggcgcgtccg actgcaggac
    13321 tgcaacgttt gcgttatctg ctgctgctgt ggggcgcgga tcgtaacggc gctaacccga
    13381 tgacgccgga agaggtgcat cgtgttgcta atgaacgtct gatgcagatg atgccgcagg
    13441 tattgccttc gccagacgcg atggggccaa agttacctga accggcaacg gggatcgctc
    13501 agccgactcc gcagcaaccg gcgacaggaa atgcagctac tgcgcctgtt gcgccgacac
    13561 agcccgctgc taatcgctct ccacaaaggg ctacgccgcc gcaaagtggt gctcaaccgc
    13621 ctgcgcgtgc gccgggaggg caatagtggc gagctatcgt agccagggac gctgggtaat
    13681 ctggctctct ttcctcattg cgctgttgct gcaaatcatg ccctggccgg ataacctgat
    13741 tgttttccgg ccaaactggg tgttacttat cttgttgtat tggatcctgg ccttgcccca
    13801 tcgcgtaaat gtgggcacag gttttgtgat gggtgccata ctggatctga tcagcggctc
    13861 gatgcttggc gtacgcgtat tggcgatgag catcattgct tacctggtgg cgctgaaata
    13921 ccagcttttc cgcaacctcg cattatggca gcaggcgctg gtcgtcatgt tgctttcgct
    13981 ggtggtggat attattgttt tctgggcaga gtttttagtg attaacgtct ctttcagacc
    14041 ggaagtgttc tggagtagtg tagtcaatgg ggtgctctgg ccgtggattt tcttgctgat
    14101 gcgtaaagtc cgtcagcagt ttgcagtgca ataaaggttt ctatgacttc tctgtattta
    14161 gcttccggtt ctccgcgtcg tcaggagtta cttgcgcaac ttggcgtgac ctttgaacgt
    14221 attgttacgg gcattgagga gcagcgtcag ccgcaggaga gcgcgcagca gtatgttgtg
    14281 cgtctggcac gcgagaaagc acaggcgggt gtcgcgcaaa cggcacagga tctcccggtg
    14341 ctgggtgcgg atattatcgt tatcctgaac ggagaagtgc tcgagaaacc gcgcgacgca
    14401 gagcatgcgg cgcagatgtt gcgcaaatta tcgggtcaga cccatcaggt gatgacggca
    14461 gtggcgttgg ccgacagcca gcacattctc gattgcctgg tggtcaccga tgtgactttc
    14521 agaacgttaa cagacgaaga catcgcgggc tatgtcgcca gcggtgaacc gttagataaa
    14581 gcaggtgcat acggtattca ggggctgggt ggctgttttg tcaggaagat aaatggcagc
    14641 tatcacgccg tagtcggctt accgctggtt gaaacgtatg aattattaag taattttaac
    14701 gcactgcgtg agaaaaggga taaacatgac ggctgaattg ttagtaaacg taacgccttc
    14761 ggaaacgcga gtggcgtata ttgatggcgg tattctgcag gaaattcata ttgaacgtga
    14821 ggcgcgacgc ggaatagtag gcaatatcta caagggtcgt gtaagtcgtg tacttccggg
    14881 tatgcaggcg gcttttgtag atattgggct ggataaagcc gcgtttcttc atgcatccga
    14941 catcatgccg cataccgaat gtgtggcggg tgaagaacaa aagcaattca cggtgcgcga
    15001 catctcggaa ctggttcgtc aggggcaaga tctgatggtg caggtggtga aagatccgct
    15061 tggcactaaa ggtgcgcgcc tgaccaccga tatcacgctg ccttctcgct atctggtatt
    15121 tatgccaggg gcttctcacg ttggggtttc ccaacgaatt gaaagcgaat cagaacgtga
    15181 acgtctgaaa aaagtggtcg cagagtattg cgacgagcag ggcgggttta tcatccgcac
    15241 cgcagcggaa ggggttggcg aggctgaact ggcctccgat gccgcttatc tgaaacgcgt
    15301 ctggaccaaa gttatggagc gtaaaaagcg cccgcagacc cgttatcagc tgtacggcga
    15361 actggcgctg gcgcagcgtg ttctgcgtga ttttgccgac gccgaactgg accgcattcg
    15421 cgttgactca cgcctgactt acgaagcatt gctggagttt acctcggagt acattcccga
    15481 gatgaccagc aagctggagc attacaccgg acgccagccg attttcgatc tctttgatgt
    15541 cgaaaacgaa atccagcgag cgttggaacg caaagtagaa ctgaaatccg gtggttatct
    15601 gattatcgac cagaccgaag cgatgaccac cgtggacatc aataccggag cgtttgtcgg
    15661 tcatcgcaat ctggacgaca ctattttcaa taccaatatt gaagcgacgc aggctatcgc
    15721 tcgccagtta cggttgcgta atctgggcgg gattatcatt attgatttca tcgatatgaa
    15781 taatgaagat caccgccgcc gcgtgctgca ttcgctggag caggcgttga gcaaagaccg
    15841 ggtgaaaacc agcgttaatg gtttttcggc gctggggctg gtggagatga cgcgtaaacg
    15901 cacccgcgaa agcattgagc acgtactgtg taacgaatgc ccaacctgcc acggtcgcgg
    15961 aacggtgaaa accgtggaaa cggtatgcta tgaaatcatg cgcgagattg ttcgtgtcca
    16021 ccatgcttac gactccgacc gtttcctggt ctatgcttct ccggcagtag ccgaagcctt
    16081 gaaaggcgaa gagtcacact cgctggcgga agtggaaatt ttcgttggca aacaggttaa
    16141 agtacaaatt gaaccgctct ataaccagga gcagtttgac gtcgtaatga tgtaaacaga
    16201 tgctgggccg ccatccggca aagggttttt gagtcacatt tttagcagac aaggagtgac
    16261 gggtgaggcg attgccgggg attttactgc ttactggagc cgcgctcgtt gtgatcgctg
    16321 ccctgctggt tagcggcctg cgtattgctt taccgcatct taacgcctgg cgtccggaaa
    16381 tcctcaacaa aatagaatcc gcgactggca tgccggtaga agccagtcag ctctcagcca
    16441 gctggcagaa ttttggcccg acgcttgaag cacacgacat ccgtgcagaa ctaaaagatg
    16501 gcggcgaatt ttcggttaaa cgcgttactc tggcgctgga tgtctggcag agcctgttac
    16561 atatgcgctg gcagtttcgc gacctcactt tctggcagct gcgctttcgc accaacactc
    16621 ctatcaccag cggtggtagc gatgacagtc tggaagccag tcacatcagc gatctgtttc
    16681 ttcgtcaatt tgaccatttc gatctccgcg acagtgaagt cagttttctg acgccatccg
    16741 gtcagcgcgc cgagctggcg atcccacaac tcacctggct gaacgatcca cgtcgacacc
    16801 gtgcggaagg cctggtaagc ctctccagcc ttaccggaca gcacggcgtg atgcaggtgc
    16861 gcatggattt gcgcgatgat gaggggttgt taagcaatgg tcgcgtctgg ctacaggcgg
    16921 atgacatcga cctgaagccg tggctcggta aatggatgca ggacaatatt gctctggaaa
    16981 cggcacagtt ctcccttgaa ggctggatga cgatcgacaa aggcgatgta accggcggtg
    17041 acgtctggct gaaacagggc ggtgccagct ggttgggcga gaagcaaacg catacgctgt
    17101 cggtggataa tctgaccgcg catattacgc gtgaaaatcc gggctggcag ttctctattc
    17161 ccgatacacg gatcacgatg gacggcaaac cctggccgag cggagcattg acgctggcct
    17221 ggataccgga acaggacgtt ggcggcaaag acaataaacg cagtgacgaa ctccggattc
    17281 gcgctagtaa tctggagctg gcaggcctgg agggcatacg cccgctggcc gcgaaacttt
    17341 cacctgcact gggtgatgtt tggcgctcca cacaaccgag cggcaagatt aacactctgg
    17401 cgctggatat cccgcttcag gcggcagaca agacccgttt tcaggcatcg tggagcgatc
    17461 tggcctggaa gcaatggaaa ttattaccgg gtgcggaaca cttctccggg acgctttccg
    17521 gcagcgttga aaatggtttg cttaccgcgt cgatgaagca ggcaaagatg ccttacgaaa
    17581 cggtattccg tgcgccactg gaaatcgccg acggccaggc aactataagc tggctgaaca
    17641 atgacaaagg tttccagctg gatgggcgta atattgacgt taaagccaaa gccgtccatg
    17701 cgcgcggcgg ttttcgttac ctgcaacctg ctaacgatga accctggctg ggtattctgg
    17761 ctggcatcag taccgatgat ggttcacaag cctggcgcta tttcccggaa aacttgatgg
    17821 gtaaagacct ggttgattat ttaagtggtg cgattcaggg cggtgaagcg gataacgcga
    17881 cgctggttta tggtggcaat ccgcaactct tcccctataa acacaacgaa ggtcagtttg
    17941 aagtgctggt gccgctgcgc aacgcgaagt ttgccttcca gccggactgg cctgcattaa
    18001 ctaaccttga tattgaactg gactttatta acgacggttt atggatgaaa accgatggcg
    18061 ttaatctggg cggcgtgcgc gcgagtaatc tcaccgcagt gatccctgac tactcaaaag
    18121 aaaaactgct gattgacgct gacattaaag gtccgggtaa agccgttggc ccttactttg
    18181 atgagacacc gctgaaagat tctctgggtg cgaccctgca agaactccag ctcgacggcg
    18241 atgtgaatgc tcgcttacat cttgatatcc cgctgaacgg cgaactggta accgcgaaag
    18301 gtgaagtgac gctgcgtaat aacagtctgt ttatcaaacc actcgacagc atcctgaaaa
    18361 atttgagcgg taaattcagc tttatcaatg gcgatctgca aagtgaacca ctgacagcaa
    18421 gctggtttaa tcagccgttg aacgtggatt tttccaccaa agaaggggca aaagcctacc
    18481 aggttgcggt gaatctcaac ggtaactggc aaccggcgaa aaccggcgtt ctgcctgaag
    18541 cggtgaacga agcattgagt ggcagcgtgg cgtgggatgg taaagtgggc attgatctgc
    18601 cttatcatgc tggcgcgacc tataacgtag agctgaacgg cgatctgaat aatgtgagta
    18661 gtcacttacc ttcaccgtta gccaaacctg cgggtgaacc actagcggta aacgttaagg
    18721 ttgatggcaa tctcaacagc tttgaattaa ccggacaggc tggtgcggat aaccatttca
    18781 atagccgctg gttgcttggt caaaagctga cgctcgatcg tgctatttgg gcggcagaca
    18841 gtaaaacgct cccgccgttg ccggaacaaa gtggcgttga acttaatatg ccgccgatga
    18901 atggtgccga gtggctggcc ctgttccaga aaggcgctgc ggagagtgtc ggtggtgcag
    18961 cgagtttccc acaacacata acgttacgta cgcctatgtt gtcactggga aatcagcaat
    19021 ggaataacct gagtattgtt tcgcaaccga cggcaaatgg caccctggtt gaggcgcaag
    19081 ggcgtgaaat caacgccacg ctagcgatgc gtaataacgc gccgtggctg gcgaatatca
    19141 aatatcttta ttacaacccg agcgtggcga aaactcgtgg tgattcaacg ccgtcatcac
    19201 ctttcccgac aacggagcgc attaacttcc gtggctggcc ggacgcacaa atacgatgca
    19261 cagagtgctg gttctggggg caaaaattcg gtcgcattga cagtgatatc accatttctg
    19321 gcgatacgtt aacgctgacc aatggactga ttgatactgg tttctcgcgg cttactgccg
    19381 atggtgaatg ggttaataat ccggggaatg aacgtacctc gctgaaagga aaactgcgtg
    19441 ggcagaaaat tgatgccgcc gcagaatttt ttggtgtcac gacgcccata cgccagtcgt
    19501 catttaatgt ggattacgat ttacactggc gcaaagcacc ctggcagcca gatgaagcga
    19561 cgttgaatgg catcattcat actcaactgg gtaaaggcga aattaccgaa atcaataccg
    19621 gacatgccgg gcaattgctg cgcttattga gcgtagatgc cctgatgcgt aagctgcgtt
    19681 ttgatttcag agacactttt ggcgaagggt tctattttga ctccattcgc agcaccgcgt
    19741 ggattaaaga cggcgttatg cacaccgacg acacgctggt ggatggcctg gaggcggata
    19801 tcgccatgaa agggtcggta aatctggtac gccgcgacct gaatatggaa gcggttgtcg
    19861 caccagagat ttctgcgacg gtgggcgtgg ctgcggcttt tgcggttaac cccattgttg
    19921 gcgcggcagt gtttgccgcc agtaaagtgc tggggccgct gtggagcaaa gtctccattt
    19981 tgcgctatca catttcgggt ccgctggacg atccgcaaat caacgaagtg ttgcgccaac
    20041 cgcgtaaaga aaaagcgcaa tgatttgacg agggcgcgta attgccccaa tctcatatga
    20101 taatcgttgc caaaggccaa cgagccggaa cataactgta ggccggatag ggcgttcacg
    20161 ccgcatccgg cagccgtaaa aaatcctcta ctgcagtaac taacgagtag caaaaacgat
    20221 gagtctgaac ctggtaagtg aacaattgct agcggcgaac ggcctgaaac atcaggactt
    20281 gttcgcgatc ctcggtcaac tggccgaacg tcgccttgat tatggcgatc tctattttca
    20341 gtcgagctat cacgaatcct gggttttaga agaccgcatt attaaagatg gttcttacaa
    20401 catcgatcag ggcgttggcg tgcgtgcaat cagcggtgaa aaaaccggat ttgcttacgc
    20461 tgaccaaatc agcctgctgg cgctggaaca gagtgcgcaa gcggcgcgca ccatcgtccg
    20521 tgatagtggt gatggcaaag tacagacgct gggcgcggta gagcatagcc cgttgtatac
    20581 ctcggtagat ccgctgcaaa gcatgagccg tgaagagaag ctggatatcc tgcgtcgcgt
    20641 cgataaggtt gctcgcgaag cggacaagcg cgtacaggaa gtgactgcca gcctcagtgg
    20701 tgtctatgaa ttaattctgg ttgcggccac cgacggcacg ctagcggcgg atgtccgtcc
    20761 gctggtgcgt ctttccgtga gcgttctggt cgaagaagat ggcaaacgcg aacgcggtgc
    20821 cagtggcggc ggcggtcgtt ttggttatga gttcttcctt gccgatcttg acggcgaagt
    20881 ccgtgcggat gcatgggcaa aagaagcagt gcgtatggcg ctggtcaatc tttctgccgt
    20941 tgctgcacca gcgggcacca tgccggtagt acttggcgca ggttggccgg gcgtgctgtt
    21001 gcatgaagcg gtaggtcacg gtctggaagg cgacttcaac cgccgtggca cttcagtatt
    21061 tagtggacag gtcggggagc tggtggcttc agaactgtgt accgtggttg atgacggcac
    21121 gatggtcgat cgccgtggtt cggtggcgat tgatgatgaa ggtacgccag gccagtacaa
    21181 cgtgctgatt gagaacggca ttctgaaagg ctacatgcag gataaactca acgcgcgttt
    21241 gatggggatg acgccgactg gcaacggtcg ccgtgaatcc tacgcccatc tgcccatgcc
    21301 gcgtatgacc aacacctata tgctgccggg taaatcgacc ccgcaagaaa ttattgaatc
    21361 cgttgagtac ggtatctatg caccgaactt tggtggcggt caggtggata tcacctccgg
    21421 caaattcgtt ttctccactt cagaagcata tctgattgaa aacggtaaag taacgaagcc
    21481 ggtgaaaggc gcaacgttga ttggttccgg tatcgaaacc atgcagcaga tttcgatggt
    21541 tggcaacgac ctgaaactgg ataacggcgt gggtgtctgc ggtaaagaag ggcaaagttt
    21601 gccggttggc gtgggccagc caacgttgaa agtcgataac ctgactgttg gcggtactgc
    21661 gtaataattt cattattttc agaaggataa ttaatctttc tacgtgcacg aacggtcccc
    21721 tcgctcctct ggggttaggg tgaggggaac ccgttggcac aggtttgtag aacgtaacag
    21781 tacaatatga attacttctc tttcccgcgc ccgtgcatct cctgaaacaa tttaccgacc
    21841 tcaacaaaat aatccgtcag cgagttgatc acgacctgta ccttcagcgg cagcttatct
    21901 ttttcggtat ataacgcata aaccgggcgt ggatctgact gataacgcgg cagcaggatc
    21961 tccagctccc cacgattgat ctcgttgatc acccacatca gcggcacgta ggcgatcccg
    22021 gcacccgccg ttagccagcg caccagcgtc atcggatcat tagtcacaaa tctcccttgc
    22081 gggatcaggc gagtcgagat cccttccggt gcaatcagtt caaattcatt gtcgggccgc
    22141 acgctgtatt caagccatga atggctactt aaatcggcgg gtttttccgg tatgccgtat
    22201 tgtgtgagat agcttttcgc ggcgcacacc accattggca tcgcgccaag acggcgggaa
    22261 aacaggctgg aatcctgcaa cgcgccgacg cggatcacca catccagacc gtcggcaatc
    22321 aggtcggggg ctggaattcc ggtaaccaga ttggcgctca aacctgggta ttctttcagc
    22381 attttggctg tcagcccggc gagaacattt tgtgccatag ttgaagaaca gccaatgcgt
    22441 agcgtcccga tgggggtgtt attgaaggca tacagttgct catgaacatc ctgcacttca
    22501 tgaagcatac gacggcagcc ctggtagtaa attctaccgg cttcggtcag gccaatgctg
    22561 cgtgtgctac ggtttaacag ctttacctgc aactcatctt ccagttttga caccgtctga
    22621 ctgatggacg aaacgctcat ctgtagctgt ctggcggcgg cggtaaaaga gccaaattca
    22681 actactttgg caaacaccga catgcgtttt agtcgttcca ttattcactc tgacttaaaa
    22741 gtgatttaga tcacataata tagataacag cataacagtt acgctaatat attaaatatc
    22801 aatctacagc aatgttgctc tcgcccggct ttccatgccc ttctctgcgg cgacagatgc
    22861 tgaaaataat aacgcctgct ctctctatac aaccaaggtc aacatgagtc tgtttcccgt
    22921 tatcgtggtg tttgggctgt ccttcccacc gatatttttt gaattgcttt tatcactggc
    22981 gattttctgg ctggtgcgcc gggtacttgt gccaacaggt atctacgact ttgtctggca
    23041 tccggcgttg ttcaacaccg cgctctattg ctgcttgttt tatttgatat cgcgactgtt
    23101 cgtttgaggt tgaagtgaaa acactaataa gaaaattctc ccgtacggcc atcacggtcg
    23161 tattagtcat tctggccttc atcgcaattt ttaatgcctg ggtctattac accgaatccc
    23221 cctggacgcg tgacgcgcgc tttagcgctg acgtcgttgc gatcgcgccg gacgtttctg
    23281 gactcattac ccaggtgaat gttcatgata accagctggt gaaaaaagga cagatactgt
    23341 tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc gatgttgctt
    23401 attatcaggt actggcacag gagaaacgcc aggaggccgg acgtcgtaac cgtctcggtg
    23461 tgcaggcgat gtctcgcgaa gagatcgacc aggctaacaa cgtactacaa acggttctgc
    23521 atcagttagc gaaagcgcag gcgacccgcg atctggcaaa actggatctt gaacgcacgg
    23581 tgatccgagc gccagcagat ggctgggtga ccaacctcaa cgtctatacc ggtgagttta
    23641 ttactcgagg atcaacggcg gttgcgctgg tgaaacagaa ctccttctat gtactggcct
    23701 atatggaaga aactaagctg gaaggggtgc gtccggggta tcgtgcagag atcacgccgc
    23761 ttggcagtaa caaagtgctg aaagggactg ttgatagtgt tgccgctggg gtcaccaacg
    23821 ccagcagcac gcgtgacgac aaagggatgg cgaccatcga ctctgacctt gaatgggtgc
    23881 gtttggcgca acgtgttccg gttcgtattc gtctcgacaa ccagcaagag aacatctggc
    23941 ctgcgggcac cactgctaca gtggtggtca ctggcaaaca agatcgcgac gaaagccagg
    24001 attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca cgatgggtat
    24061 tttctccatt gctaaccaac atattcgctt tgcggtaaaa ctggcgaccg ccattgtact
    24121 ggcgctgttt gttggctttc acttccagct ggaaacgcca cgctgggcgg tactgacagc
    24181 ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt gaaccgtatt ctggcgctat
    24241 tcgctatcgt ggctttttgc gcatcatcgg cacatttatt ggctgtattg ccggactggt
    24301 gatcatcatt gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct gtatctgggc
    24361 cggtttttgt acctggatat cctcgctggt acgaatagaa aactcgtacg cgtgggggct
    24421 ggccggttat accgcgctga tcattgtgat caccattcag ccggaaccat tgcttacgcc
    24481 gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg cgattatggc
    24541 ggatttgctc ttttctccgc gatcgatcaa acaagaagta gatcgagagc tggaaagttt
    24601 gctggtcgcg caatatcaat taatgcaact ctgtatcaag catggcgatg gtgaagttgt
    24661 cgataaagcc tggggcgacc tggtgcgacg caccacggca ctacaaggca tgcgcagcaa
    24721 cctgaatatg gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag cgatcaatac
    24781 gctatcgctg acgctgatta cccaatcctg cgaaacttat cttattcaga atacgcgccc
    24841 ggaattgatc actgatactt tccgcgaatt ttttgacacg ccggtagaaa ccgcgcagga
    24901 cgtccacaag cagctcaaac gcctgcggag agttatcgcc tggactgggg aacgggaaac
    24961 gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt tatcagcttc tcaagcgcgg
    25021 cgttatcagt aacacaaaaa tcaacgccac cgaagaagag atcctgcaag gcgaaccgga
    25081 agtaaaagta gagtcagccg aacgtcatca tgcaatggtt aacttctggc gaaccacact
    25141 ttcctgcatt ctgggcacgc ttttctggct gtggacgggc tggacttccg gcagtggtgc
    25201 aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt ttgccgaatc cacgcatggt
    25261 ggcgatcgac tttatctatg ggacgctggc cgcgctgccg ttagggctgc tctacttttt
    25321 ggtgattatc cctaataccc aacagagcat gttgctgctg tgtattagcc tggcagtgct
    25381 gggattcttc ctcggtatag aagtacagaa acggcgactg ggctcgatgg gggcactggc
    25441 cagcaccata aatattatcg tgctggataa cccgatgact ttccatttca gtcagtttct
    25501 cgacagcgca ttaggacaaa tcgtcggctg tgtgctcgcg ttcaccgtta ttttgctggt
    25561 gcgggataaa tcgcgcgaca ggactggacg tgtactgctt aatcagtttg tttctgccgc
    25621 tgtttccgcg atgactacca atgtggcacg tcgtaaagag aaccacctcc cggcacttta
    25681 tcagcagctg tttttgctga tgaataagtt cccaggggat ttgccgaaat ttcgcctggc
    25741 gctgacgatg attatcgcgc accagcgcct gcgtgatgcg ccgatcccgg ttaacgagga
    25801 tttatcggcg tttcaccgac aaatgcgccg cacagcagac catgtgatat ctgcccgtag
    25861 cgatgataaa cgtcgtcggt actttggcca gttgctggaa gaactggaaa tctaccagga
    25921 aaagctacgc atctggcaag cgccaccgca ggtgacggaa ccggttcatc ggctggcggg
    25981 gatgctccat aagtatcaac atgcgttgac cgatagttaa gtcaaaaccg acgccaaaag
    26041 cgtcggtttt ttcatggcta tacttagcga tgcacggcag aactcgccgc gaaacgtgac
    26101 ggtggcaaca gatgaatatt tatgcctttg attttgatga gattgagagt caggaggatt
    26161 tttatcgtga ctttagccaa acctttggtc tggctaaaga taaggtacgc gatctcgact
    26221 cactatggga tgtgttaatg aacgatgtcc tgccgctacc acttgagatt gaatttgttc
    26281 atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt gatgaggcag
    26341 aggaagagct ggaagggcat ttgcgtttta atgttcgtca ttagcgcaaa aaaaagccca
    26401 cgaaccgggg gcaatatcgt cggacaaaac gatgagggtt tatttgtaca gttcagccgt
    26461 agcgtgccag gtgtcaccgc tacgagcttc agtaatctgg taggccgttg cgcctttctc
    26521 ttccgctttt ttgttcagca tttcacgtat atccattggc gaagacgcca caccacttac
    26581 ggatacggtc ccgattgctt cacgattttg tgcttgtgca gcatcaatgg agtcggcagc
    26641 gaatgcaccg aaagagagaa cagaaagtac gcttaatgca gctacagtgg ttttgatttt
    26701 catgattttt acctcgacat aatcttttag ctgggccttt gtttcgtgac ccatatcaca
    26761 aaatcaagta tacactaatt attgaactaa ttaataccac actaactgtt tcagttgata
    26821 ctaagtgtta aaaatgtgaa ttttaataac aaaaaagtat aaaaaacacg gttatttatt
    26881 attaattata gtattttcaa ttagataatg aattttgcat aatgtgcttt ttttaccgta
    26941 tgtattcatt ttgtgaatga tgtgtcgcgg taaaacgcac tattcgttaa atgtatcgca
    27001 ggagaaagaa ggggaatgag agggataagc aacattttcc ccgccgccag aagcgacggg
    27061 gcagagatta aagctcctgg tcgaacagct ctaaaatcgc ttcgtacagg tctttgacgg
    27121 tgaaaccgtt agcaggggtg gtaaagatgg tgtcatcgcc agcgatggtg cccagaatac
    27181 cttctgcttt gcccagtgag tccagcaggc gagcaattaa ctgcgccgcg ccagggctgg
    27241 tatgaatcac gacaactgca tcgttgtagt cgatatccag caccagattc ttcaatggac
    27301 tggaggtggt tggtacaccc agttcagctg gcaggcagta aaccatttcc attttggcat
    27361 tgcgtgtacg tacagcacca aacttggtca acatccgcga gactttagac tgattaatat
    27421 tgtcaaagcc ttgctcctgc aacgcggcga cgatttcgcc ctgggagcta aatttctctt
    27481 ctttaagtaa tgctttaaat gctttaacta gttcttcttg cttagccgag cttcgcataa
    27541 gtcacccgat atggtggttg atacaacatt attgtgcata cagatgaatt tttatgcaaa
    27601 cagtcagccc tgaagaaggc tgaaataatg ttatgaaaga gcgggatttt atcaaatttc
    27661 gttattgaga aacatgcctg cgtcacggca tgcaaattct gtttaaaagt aaattaattg
    27721 ttatcaaatt gatgttgttt tggctgaacg gtagggtata ttgtcaccac ctgttggaat
    27781 gttgcgctaa tgcataagcg actgttaatt acgtaagtta ggttcctgat tacggcaatt
    27841 aaatgcataa acgctaaact tgcgtgacta cacattcttg agatgtggtc attgtaaacg
    27901 gcaattttgt ggattaaggt cgcggcagcg gagcaacata tcttagttta tcaatataat
    27961 aaggagttta ggatgaaagt cgcagtcctc ggcgctgctg gcggtattgg ccaggcgctt
    28021 gcactactgt taaaaaccca actgccttca ggttcagaac tctctctgta tgatatcgct
    28081 ccagtgactc ccggtgtggc cgtcgatctg agccatatcc ctactgctgt gaaaatcaaa
    28141 ggtttttctg gtgaagatgc gactccggcg ctggaaggcg cagatgtcgt tcttatctct
    28201 gcaggcgtag cgcgtaaacc gggtatggat cgttccgacc tgtttaacgt taacgccggc
    28261 atcgtgaaaa acctggtaca gcaagttgcg aaaaactgcc cgaaagcgtg cattggtatt
    28321 atcactaacc cggttaacac cacagttgca attgctgctg aagtgctgaa aaaagccggt
    28381 gtttatgaca aaaacaaact gttcggcgtt accacgctgg atatcattcg ttccaacacc
    28441 tttgttgcgg aactgaaagg caaacagcca ggcgaagttg aagtgccggt tattggcggt
    28501 cactctggtg ttaccattct gccgctgctg tcacaggttc ctggcgttag ttttaccgag
    28561 caggaagtgg ctgatctgac caaacgtatc cagaacgcgg gtactgaggt ggttgaagcg
    28621 aaagccggtg gcgggtctgc aaccctgtct atgggccagg cagctgcacg ttttggtctg
    28681 tctctggttc gtgcactgca gggcgaacaa ggcgttgtcg aatgtgccta cgttgaaggc
    28741 gacggtcagt acgcccgttt cttctctcaa ccgctgctgc tgggtaaaaa cggcgtggaa
    28801 gagcgtaaat ctatcggtac cctgagcgca tttgaacaga acgcgctgga aggtatgctg
    28861 gatacgctca agaaagatat cgccctgggc aaagagttcg ttaataagta attgattagc
    28921 gaataataaa aaaccggagc acagactccg gtttttgttt tgagcactcg acttaattgg
    28981 ttgccggata ttcctgaatg gtgacctgca gcgttaactg cttatcatca cgcatcacta
    29041 caaccgggat caccgaacca gggcgaattt ccgccacctg atccatcgtc tccagagcag
    29101 agatggccgg tttgttatcc accgaaataa tcagatcgtt gacctgaata cccgcattcg
    29161 ccgctgggcc gtcaggtgac acttcattaa ccacgatccc ttgcagttga tctataccac
    29221 cgctctgcgc gtgcagtggt gcgatctcgc gtccgccgat accaatgtag ccgcggatca
    29281 cgcgaccatc gcggatcagc ttatccataa ttttggttgc taactggaaa ggaatcgcaa
    29341 agccgatacc ttccggcgtt tcgccatcgt tactcttatc aaacgacagc gtgttaatgc
    29401 ccatcagttc tcccagcgag ttcaccagcg cgccgccaga gttaccgtgg ttaatggaag
    29461 catcggtttg taggaagttt tgccgcccgg tcgggttcag accgattcga cccgtggcac
    29521 taataatccc ttgggtaatg gtctgcccga ggttgtacgg gttaccgatc gccagtacta
    29581 cgtcgccaat gtgcggtacg cgacgtgcat taattggaat ggtaggtaaa ccgccagtgg
    29641 cattaatttt aagtaccgcc agatcggtta gagagtcaga tcccaccagc aatgcttcaa
    29701 atacacgtcc atcctgtaag gcgacgatga tctgatcggc gtcgttgatg acgtgtttat
    29761 tggtgatgat ataaccgcgt tgatccatga ttacaccgga tcccagggtg cggatctcaa
    29821 gctggttgtg agagttggtg ttcaaaccac ggttgtaaac gttaaccacc gctggcgcgg
    29881 cgcggcgaac cgccagatta tagctggcag gcgtctcatc ggtactgtca aattgcggag
    29941 tggaaagcgg gttaaggctg cgcagcgaag gcatggcaac cagcagaata gcgccgacaa
    30001 ttaatccaat cgcaacggaa cgtaagagct tcacaaacat gatggaggcg tcattaaaaa
    30061 agggaacggc agcagcatac cacgagttaa ccggacatca cacgtcagcc tgatgcccgg
    30121 tttacgacat taacgcatca gcagatagat gctttcattg ccgcgtacaa tttgcagggc
    30181 gatgatggcc ggttttgccg ccagcacttt acgcatttca gcaatcgagt tcacccgatc
    30241 gcggttgacg ccaatgatca catcgtcttt ttgcaagcca gcctgagcag ctgggcttcc
    30301 tttgacaact tcatctatct taataccttt gccgccatct tttagctgac catcgctcaa
    30361 cgttgcacct tccagcgctg gccgacgaag aggtgctggt atcaagcgtc acttctactt
    30421 ccagtggttt gccgttacgc agcaggccaa gcttcacttt cgtgcccggc tctgtagtcg
    30481 cgatacgaga gcgcaactca gcaaagctat tcagcggttt gccgttgagg ctggtaataa
    30541 tatcgcccgc tttgacgccc gctttcgctg agccagaact tggcaacact tcgctgacaa
    30601 acgcgccacg ctgcacgtca aggttgaagg ctttggcgat atcggcactc atctcggtgc
    30661 ctttgatgcc taacaaaccg cgtttgattt caccaaagtc gataagctgc tgcgccagtg
    30721 ttcgcgccat attactgggg atggcaaatc caatcccgac gctcccgccg ccaggcgcaa
    30781 ggattgcagt gttgatgcca attaactcac cgttaaggtt taatagtgca ccgccggagt
    30841 taccgcggtt aatggaagca tctgtctgga taaagttttc cagaccttca agattcaacc
    30901 cgctgcggcc taatgcggaa acaatgccag aggtggcggt ttgcccaagg ccaaatgggt
    30961 taccgaccgc tacggcaaaa tcaccgacgc gcaatttatc ggagtcggca atagcgattt
    31021 gcgttaattt gctcgggttt tgaatttgta acagggcgat atcgctctgg tcatcgctac
    31081 caatcagttt tgcatcaaac tcgcgcccat cattgagctg aatactgatt ttctgtgcct
    31141 gattaatcac atggttgttg gtcagcacat agcctttact ggcgttgatg atgacaccgg
    31201 aacctaaacc ttcgaagggt tgtgcaggtt gatccggtaa atcatcacca aaaaactttt
    31261 tgaattcttc cgggattttc tgtccctgac tggccgttcc ttccacccgt acgctcacca
    31321 ctgccggaag cactttttcc agcattggag ccagactggg gagaggggcc tgatcggcaa
    31381 cctggcctgg aatcgacgcg acggcctgaa atgacgccga gagagttaac ccggcactta
    31441 acgctaatgc actcaacagc tgggtttgtt ttttcattat tcctgctctc gtacctgaat
    31501 gataagaaaa gagattcaaa acagtttgat gttattgaat tttcagacgg ctaacaatga
    31561 gacaccagat taaatgatag tcggggaggg agagaagagg agggcgcaat ggctgcgccc
    31621 gaaaaataaa ttagtcgcgc ttcgcgccag tacgcagcag gccggatgcg ccttcagaat
    31681 agtcgcgagg cacctgcacc ggtgcctgat cgttgctggc ttcagactct gccagacgat
    31741 tacggaacgg gtttgcttca gcagacagtt ccggcagcag gctgctggag ctttttgcca
    31801 tgtgctgata cagctggcga tagtcgtgcg ccatggtatc cagtaattcc gcgctgcggg
    31861 caaagtggct aaccagctct tcgcgatact cgtccagttc agctttattc ttttccagtt
    31921 cgtactgcaa cgcctgttgc tggcgtagtt tacgattacc aaaacgcatg gccacagcac
    31981 caataatgat gccgacgact aacccaatta gcgcatattc ccaggtcatg aacatctccc
    32041 gttgtctttt gtttccgtag ggtgttggct tcaggctcca tgcctgcggc tgattatgcc
    32101 actataaccg ttaattccac agaagtggaa tcccgactgc atatcgcgta gtgtagaacg
    32161 gccttttttt cgtcaaccgt gaacaacggc gcaccgatta ttcaaggaat aacaataaga
    32221 tcatgcaaag cgttacccca acatcgcaat acctgaaggc gctcaatgaa ggcagccatc
    32281 aacccgacga cgttcaaaaa gaggccgtca gccgcctgga aattatttat caagaactca
    32341 tcaatagcag gccaccagcc cccaggacga gtgggctaat ggcgcgggtc ggtaagctgt
    32401 ggggtaaacg cgaagacaca aagcatacgc cagtgcgtgg cttatatatg tggggcggtg
    32461 taggacgcgg gaaaacctgg ctgatggacc ttttctatca aagcctgccg ggagagcgga
    32521 aacagcgcct gcactttcac cgttttatgc tgcgggtgca cgaagagcta actgccttac
    32581 aggggcagac cgatccgctg gaaattattg ccgatcgctt taaagccgaa actgacgtgc
    32641 tctgttttga cgaatttttt gtttctgata ttaccgacgc catgctactt ggcggtctga
    32701 tgaaagccct gttcacccgc ggtattaccc tggtagcgac gtcaaatatt ccgccggacg
    32761 aactttatcg aaatggcctg caacgtgcgc gttttctgcc tgcaatcgat gccattaaac
    32821 agcattgtga tgtaatgaac gtggacgctg gtgttgatta tcgactgcgt acactcactc
    32881 aggcgcatct gtggctttcg ccactcaacg atgaaacccg ggcgcagatg gataaactat
    32941 ggttggcgct ggcgggggtg aaacgagaaa attcaccgac gttagaaatc aaccatcggc
    33001 cattggcgac aatgggcgtc gagaaccaga cgctggcggt ttcttttact acgctgtgcg
    33061 tcgacgcccg cagtcagcat gactatattg cgctctcacg cctgtttcac acagtaatgt
    33121 tgtttgatgt gccagtcatg acgcggttga tggagagcga agcgcggcgc tttattgcgc
    33181 tggtggatga gttttacgag cgccatgtca aattagtggt gagtgcagaa gtgccgctgt
    33241 atgaaattta tcagggtgag cggctgaagt ttgagttcca gcgttgcctg tcacgtctgc
    33301 aagagatgca aagcgaagag tatctgaagc gcgagcattt ggcgggttaa aacctgccac
    33361 aaatcacaaa aaggggtcga tctttgaccc cgacttctct ataatcctgc gaccccacgt
    33421 tacaagaaag tttttttccc aaaacttttt gtgtgctggc ataggctatt cgaaggggta
    33481 ggtttgccgg actttgtcgt gtgaacctca acaattgaag acgtttgggt gttcaccaac
    33541 gtgtaactat ttattgggta agcttttaat gaaaactttt acagctaaac cagaaaccgt
    33601 aaaacgcgac tggtatgttg ttgacgcgac cggtaaaact ctgggccgtc tggctactga
    33661 actggctcgt cgcctgcgcg gtaagcacaa agcggaatat actccgcacg tagataccgg
    33721 tgattacatc atcgttctga acgctgacaa agttgctgta accggcaaca agcgtactga
    33781 caaagtgtac tatcaccaca ccggccacat cggtggtatc aaacaagcga cctttgaaga
    33841 gatgattgct cgccgtcctg agcgtgtgat tgaaatcgcg gttaaaggca tgttgccaaa
    33901 aggcccgctg ggtcgtgcta tgttccgtaa actgaaagtt tacgcgggta acgagcacaa
    33961 ccacgcggca cagcaaccgc aagttcttga catctaatcg ggattatagg caatggctga
    34021 aaatcaatac tacggcactg gtcgccgcaa aagttccgca gctcgcgttt tcatcaaacc
    34081 gggcaacggt aaaatcgtaa tcaaccaacg ttctctggaa cagtacttcg gtcgtgaaac
    34141 tgcccgcatg gtagttcgtc agccgctgga actggtcgac atggttgaga aactggacct
    34201 gtacatcacc gttaaaggtg gtggtatctc tggtcaggct ggtgcgatcc gtcacggtat
    34261 cacccgcgct ctgatggaat acgacgagtc cctgcgttct gaactgcgta aagctggctt
    34321 cgttactcgt gacgctcgtc aggttgaacg taagaaagtc ggtctgcgta aagcacgtcg
    34381 tcgtccgcag ttctccaaac gttaattggc ttctgctacg gcagaaaaca atttgcgaaa
    34441 aaacccgctt cggcgggttt ttttatggct aaattctgaa tcagcgtaaa aaatgggaag
    34501 ttgctttttg ctgccgtctg aaagacaggt aaaacaaaaa ccacatcgcc aataagggtc
    34561 taagtcaact atttcagact aaagcgcatc tctttttccc catttccggc atccactcac
    34621 cacaaaggtc gcaaaatctg gtaaactatc atccaatttt ctgcccaaat gtcgggtatt
    34681 gctcattttt tgtttgattt tcgaacaaag agagtcgttc cttattgggt aacacaactt
    34741 ctgactggcc acctggtggc tggtagcagt aaaaattctg actatacctg gaggttttca
    34801 tggctgtcgc tgccaacaaa cgttcggtaa tgacgctgtt ttccggtcct actgacatct
    34861 atagccatca ggtccgcatt gtgctggctg agaaaggtgt aagtttcgag atcgaacacg
    34921 tggaaaagga caatccgcct caggatctga ttgacctcaa cccgaatcag agcgttccga
    34981 ccctggtgga tcgtgagctg accctgtggg aatctcgcat cattatggaa tatctggatg
    35041 agcgtttccc gcatccgcca ctgatgcctg tttacccggt agctcgcggt gaaagccgtc
    35101 tgtacatgca tcgcatcgaa aaagactggt acacgctgat gaacactatc atcaacggtt
    35161 cagcttctga agcagatgcc gcacgtaagc aactgcgcga agaactgctg gcgattgcgc
    35221 cggtcttcgg tcagaagccg tacttcctga gcgatgagtt cagcctggtc gattgctacc
    35281 ttgctccgct gctgtggcgt ctgccgcaac tgggcatcga gttcagcggc ccgggtgcga
    35341 aagagctgaa aggctatatg acccgcgtct ttgagcgtga ctctttcctt gcttctttaa
    35401 ctgaagcaga acgtgaaatg cgtctgggcc ggagttaatc tgtatggatt tgtcacagct
    35461 aacaccacgt cgtccctatc tgctgcgtgc attctatgag tggttgctgg ataaccagct
    35521 cacgccgcac ctggtggtgg atgtgacgct ccctggcgtg caggttccta tggaatatgc
    35581 gcgtgacggg caaatcgtac tcaacattgc gccgcgtgct gtcggcaatc tggaactggc
    35641 gaatgatgag gtgcgcttta acgcgcgctt tggtggcatt ccgcgtcagg tttctgtgcc
    35701 gctggctgcc gtgctggcta tctacgcccg tgaaaatggc gcaggcacga tgtttgagcc
    35761 tgaagctgcc tacgatgaag ataccagcat catgaatgat gaagaggcat cggcagacaa
    35821 cgaaaccgtt atgtcggtta ttgatggcga caagccagat cacgatgatg acactcatcc
    35881 tgacgatgaa cctccgcagc caccacgcgg tggtcgaccg gcattacgcg ttgtgaagta
    35941 atacaaaaca ggcccaggcg gcctgttttg tctttttaat gacttaataa aatagcgtga
    36001 ttgtggcaat catattcacc acgaaaccga ctgccatggg gatcgctgtc cgcttcacca
    36061 ccgcaaaagg cgaaacgcct gcaattcctg acgtaacgac caccaccgca gtaatcggcg
    36121 aaaccgcgcg cgctagcgtc gtggcaaaat gcatcggcat aatcattaca accgctggta
    36181 catgtagtcc ggctgcgata ttcggaataa gactggcaaa tgacataaac ggcgcattgc
    36241 cagagcccat cacaatggca caaatggcaa tgactagcgc cataataatc atcacgccaa
    36301 taccgcccag accagaatgc tccgcacccc tgataaccgc atcaacagtg ccaatcgtcg
    36361 ttaagccttt cgcaaatatt tcacccgcga ccaccagcgt taccacgttg acaaactgcg
    36421 tacccatgcc gtcaaaaaac gcctgcacat cgtccattgt ttcgcgcaag ttatgcttgc
    36481 ggaagaactc aacaaacatc gtcaccgtca aacttagtaa catcaccacc accagatgca
    36541 gttctgactg catcagcccg acgtgggcga ggaacagcga gccgagcatc aggattaacg
    36601 gcatcacagg taaaatggcg taatagagcg gcgggacatt atcgagagtt ttttgctctg
    36661 cctgttcgtg attgatattt ttatcttttt tgtcaaaagc gcgttgcacg aaaaagtggg
    36721 agatcgccac cgagataatg acgcaagagg cgaccggaag ctggtagtgg aagaagtatg
    36781 tggcaatttt cattcccgcg acctgggctg caaaaatgga gttcgtttcc agaatccccc
    36841 actcaatgga catcgtggtt gcgataaccg ccaccgcaga gaggcgactt actcccagac
    36901 tcaccagcgt cggaaataag gtgaccatca gcaacatacc cagaccggag gcgctggtaa
    36961 taaactgcgc catgatttgg ccgatgacgt aagttgccga cagaataata tacggcgagc
    37021 gaatgagttt taacgggcgg cttagcaagc tcaccatcgc gcaactggct cctatgcgct
    37081 ccatgtagcg ggcataaccg cccaccgcca taatcgacag ccccaatccg gcaatgcggt
    37141 tggacagcat gcgcaggatt tcattataaa tgtcgacgat caagtagccg ctgcttttgg
    37201 cgggatcgag tacaccaccg aaccctaacc agactccgca catcatcagg aagacacctg
    37261 cggcagccag caccacctga ggtttgtagt ttttcaggat caaatagccc atcgtaatta
    37321 atacgatgac agatataatt at
//
LOCUS       DAJTYM010000031        35225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTYM010000031 DAJTYM010000000
VERSION     DAJTYM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..528)
                     /gene="deoR"
                     /locus_tag="OO575_001586"
     CDS             complement(<1..528)
                     /gene="deoR"
                     /locus_tag="OO575_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6664593.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGV"
     gene            complement(586..1182)
                     /gene="ybjG"
                     /locus_tag="OO575_001587"
     CDS             complement(586..1182)
                     /gene="ybjG"
                     /locus_tag="OO575_001587"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6664594.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1467..2693
                     /gene="mdfA"
                     /locus_tag="OO575_001588"
     CDS             1467..2693
                     /gene="mdfA"
                     /locus_tag="OO575_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6664595.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(2734..3018)
                     /locus_tag="OO575_001589"
     CDS             complement(2734..3018)
                     /locus_tag="OO575_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664596.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3104..3919)
                     /gene="ybjI"
                     /locus_tag="OO575_001590"
     CDS             complement(3104..3919)
                     /gene="ybjI"
                     /locus_tag="OO575_001590"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6664597.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(3919..5127)
                     /locus_tag="OO575_001591"
     CDS             complement(3919..5127)
                     /locus_tag="OO575_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6664598.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5211..5747
                     /gene="rcdA"
                     /locus_tag="OO575_001592"
     CDS             5211..5747
                     /gene="rcdA"
                     /locus_tag="OO575_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6664599.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(5922..7607)
                     /locus_tag="OO575_001593"
     CDS             complement(5922..7607)
                     /locus_tag="OO575_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6664600.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            7877..8254
                     /locus_tag="OO575_001594"
     CDS             7877..8254
                     /locus_tag="OO575_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6664601.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8284..8541)
                     /gene="grxA"
                     /locus_tag="OO575_001595"
     CDS             complement(8284..8541)
                     /gene="grxA"
                     /locus_tag="OO575_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6664602.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8701..8988
                     /locus_tag="OO575_001596"
     CDS             8701..8988
                     /locus_tag="OO575_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6664603.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            8972..9694
                     /gene="nfsA"
                     /locus_tag="OO575_001597"
     CDS             8972..9694
                     /gene="nfsA"
                     /locus_tag="OO575_001597"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6664604.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            9755..10657
                     /gene="rimK"
                     /locus_tag="OO575_001598"
     CDS             9755..10657
                     /gene="rimK"
                     /locus_tag="OO575_001598"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6664605.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            10745..11221
                     /locus_tag="OO575_001599"
     CDS             10745..11221
                     /locus_tag="OO575_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6664606.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11573..12685
                     /gene="potF"
                     /locus_tag="OO575_001600"
     CDS             11573..12685
                     /gene="potF"
                     /locus_tag="OO575_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6664607.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            12780..13913
                     /gene="potG"
                     /locus_tag="OO575_001601"
     CDS             12780..13913
                     /gene="potG"
                     /locus_tag="OO575_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6664608.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            13923..14876
                     /gene="potH"
                     /locus_tag="OO575_001602"
     CDS             13923..14876
                     /gene="potH"
                     /locus_tag="OO575_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6664609.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            14873..15718
                     /gene="potI"
                     /locus_tag="OO575_001603"
     CDS             14873..15718
                     /gene="potI"
                     /locus_tag="OO575_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6664610.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            15778..16266
                     /locus_tag="OO575_001604"
     CDS             15778..16266
                     /locus_tag="OO575_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6664611.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16307..17434
                     /gene="rlmC"
                     /locus_tag="OO575_001605"
     CDS             16307..17434
                     /gene="rlmC"
                     /locus_tag="OO575_001605"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6664612.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17609..18340)
                     /gene="artJ"
                     /locus_tag="OO575_001606"
     CDS             complement(17609..18340)
                     /gene="artJ"
                     /locus_tag="OO575_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6664613.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18632..19300)
                     /gene="artM"
                     /locus_tag="OO575_001607"
     CDS             complement(18632..19300)
                     /gene="artM"
                     /locus_tag="OO575_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6664614.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19300..20016)
                     /gene="artQ"
                     /locus_tag="OO575_001608"
     CDS             complement(19300..20016)
                     /gene="artQ"
                     /locus_tag="OO575_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6664615.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20023..20754)
                     /gene="artJ"
                     /locus_tag="OO575_001609"
     CDS             complement(20023..20754)
                     /gene="artJ"
                     /locus_tag="OO575_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6664616.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(20772..21500)
                     /gene="artP"
                     /locus_tag="OO575_001610"
     CDS             complement(20772..21500)
                     /gene="artP"
                     /locus_tag="OO575_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6664617.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(21718..22233)
                     /locus_tag="OO575_001611"
     CDS             complement(21718..22233)
                     /locus_tag="OO575_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6664618.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            22882..24651
                     /locus_tag="OO575_001612"
     CDS             22882..24651
                     /locus_tag="OO575_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6664619.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            24862..25185
                     /locus_tag="OO575_001613"
     CDS             24862..25185
                     /locus_tag="OO575_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6664620.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25182..26012
                     /gene="amiD"
                     /locus_tag="OO575_001614"
     CDS             25182..26012
                     /gene="amiD"
                     /locus_tag="OO575_001614"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6664621.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26009..27022)
                     /locus_tag="OO575_001615"
     CDS             complement(26009..27022)
                     /locus_tag="OO575_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6664622.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27121..28551)
                     /locus_tag="OO575_001616"
     CDS             complement(27121..28551)
                     /locus_tag="OO575_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6664623.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28562..29563)
                     /gene="ltaE"
                     /locus_tag="OO575_001617"
     CDS             complement(28562..29563)
                     /gene="ltaE"
                     /locus_tag="OO575_001617"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6664624.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29600..31318)
                     /gene="poxB"
                     /locus_tag="OO575_001618"
     CDS             complement(29600..31318)
                     /gene="poxB"
                     /locus_tag="OO575_001618"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6664625.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31451..32419)
                     /gene="hcr"
                     /locus_tag="OO575_001619"
     CDS             complement(31451..32419)
                     /gene="hcr"
                     /locus_tag="OO575_001619"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6664626.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32431..34083)
                     /gene="hcp"
                     /locus_tag="OO575_001620"
                     /gene_synonym="priS"
     CDS             complement(32431..34083)
                     /gene="hcp"
                     /locus_tag="OO575_001620"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6664627.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34227..35126)
                     /gene="lysO"
                     /locus_tag="OO575_001621"
     CDS             complement(34227..35126)
                     /gene="lysO"
                     /locus_tag="OO575_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6664628.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8774 a         8706 c         9059 g         8686 t
ORIGIN      
        1 cacgcccgcc gcggaataaa aagcgatatc cgggcaaaaa ttattcaacg tttgctgaaa
       61 atcgatgggt ttgaaaatgg cgttgctggc gtgaaattca ccaccgcaca gaaatgcgcg
      121 gcaatggggt ttctctttca gcgccagaaa ggtatttagc gaataacaaa cggcggtaaa
      181 agggatttca ttatcaatcg cttcaataat ccacggcgtg gtggtgccac agtcaaaaaa
      241 gagggtctga tcgggttcta ccagtgtcgc agccagtttt gccgcccggc gtttttcttc
      301 caccaggcgg gatttttgat cgcttaacag gtaatggctg gcactgcgcg gttccagaac
      361 aatatagccg ccgagcaaaa cgacgggcgc actgtggttg ttcagatcgc gacgaatcgt
      421 catctccgaa accccaagca gggcggcggc gtctttaaga tgtaacttat cgctgcgttt
      481 taattcttgc agcagctgcc cgatacgctc ttcgcgacgt gtttccataa tccctctgaa
      541 tagttattga agcgagccgc tcaatactac actttttagc agagatcagt cacgcaccca
      601 gcctttgcgg atcggtaatg caaaacagac gcgataccac gattgcagac gttgatagag
      661 tttatgcccc atcgcttgcc agataatctg ggcactaagg cagccaatca tacctgccag
      721 caatccaccg agcatatcca gcggccagtg gacgccaaga taaacgcgcg accaggcaat
      781 gacgacggcc agcaccatta aaagtgagcc ggaccacagg cgatgccagc ataaaaatgc
      841 cagtgcaaag gtgaaaatca ccgtaccgtg atcgctcggg aatgagtcat ccgccgcatg
      901 atgcaggaag ttatagccga tattttcgac aaagggtcgg tcgtgcggaa aaagatgtcc
      961 catcgtccag gacacaaaca ggctgaccgc cagcgcgata gcgattttta tcaccagttg
     1021 ccgttgtgct gtaagccccc acaaccaaag taccgcggcc agcaacggca ccacggtaat
     1081 caaatcttta gcaataaaaa tcgccaacga gatcatccac ggagccgagt ctggcgtcgc
     1141 gttaataaga gagaatagag agagattcaa attttccagc ataacttccc gacgcaaagt
     1201 gattaaaagg gagccaatac aggcaagtcg ttgagaataa agtgcaggtt aaactgggta
     1261 aagcggcatc gtcttatttc cctcaagcgg cctgtttacg gtgggtgatt gtaacgggca
     1321 taggttaaat aaaacttaaa gaaagcgtag ctatactcgc aataatgtaa gaatgtgctt
     1381 aaccgtggtt tcagctacaa aattcgcttt ctcgttagct gcgcttttat taaactctgc
     1441 gcgattatta ttggcgaaga aattgcatgc aaaataaatt agcttccggt gccaggcttg
     1501 gacgtcaggc gttacttttc cctctctgtc tggtgcttta cgaattttca acctatatcg
     1561 gcaacgatat gatccaaccc ggtatgttgg ccgtggtgga acaatatcag gcgggcattg
     1621 attgggttcc tacttcgatg accgcgtatc tggcgggcgg gatgttttta caatggctcc
     1681 tggggccgct gtcggatcgt attggtcgcc gtccggtgat gctggcgggt gtggtgtggt
     1741 ttatcgtcac ctgtctggca atattactgg cgcaaaacat tgaacaattc accctgttgc
     1801 gcttcttgca gggcataagc ttctgtttca ttggcgctgt gggatacgcc gcaattcagg
     1861 aatccttcga agaggcggtt tgtatcaaga tcaccgcgct gatggcgaac gtggcgctga
     1921 ttgctccgct acttggtccg ctggtgggcg cggcgtggat ccatgtgctg ccttgggagg
     1981 ggatgtttgt tttgtttgcc gcattggcag cgatctcctt tttcggtctg caacgagcta
     2041 tgcctgaaac cgccatgcgt ataggcgaga aactgtcact gaaagaactc ggtcgtgact
     2101 ataagctggt gctgaagaac ggccgctttg tggcgggggc gctgggattc gttagcctgc
     2161 cattgctggc gtggatcgcc cagtcgccga ttatcatcat taccggggag cagttgagca
     2221 gctatgaata tggcttgctg caagtgccta ttttcggggc gttaattgcg ggtaacttgc
     2281 tgttagcgcg tctgacctcg cgccgcaccg tacgttcgct gattattatg ggcggctggc
     2341 cgattatgat tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt
     2401 tatggatgac cgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac
     2461 tggtgcgatt aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg
     2521 gaatgctgca aatgctgatc tttaccgtcg gtattgaaat cagcaaacat gcctggctga
     2581 acgggggcaa cggactgttt aatctcttca accttgtcaa cggaattttg tggctgtcgc
     2641 tgatggttat ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg
     2701 cctgattgat ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg
     2761 ttcgataacc atcccttcac ctacgctggc aagatggcga acataaggat gcgggcggta
     2821 agccggagct ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc
     2881 ttgtggaacg ctgactgaat caggaaccac aaccactttg tatccactcg gcacatcgac
     2941 ggtgacactt tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag
     3001 acaatttttc ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg
     3061 cagccttcat tcaggcattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg
     3121 cttcgtgttt aagaacttta tcgatcacat ccagtacgcc ttcacggtta ttgtagcctg
     3181 cccggtattt tgccgctgcg acgactgcgc tgccggcatt ttccattgca aaactaaagc
     3241 ctgcctgacg cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt
     3301 cgtctattcc ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga
     3361 taatcagatc gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct
     3421 catgtaatgc tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa
     3481 agaaaatatc ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt
     3541 ccgccaccgt tttcatggca tcgtcatatt ttttgagtgt ataggcgcta ttttttccgc
     3601 aggcaataat ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gcaaacgcat
     3661 ccttcgacag ctcgccatta aaaacatctt tgccttcact cattacccag ccgccgtttt
     3721 cagccacaaa ggcaatttca ttcgcaattt cagggaagaa agagatcaac tgataatatt
     3781 gattcccgct ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca
     3841 taaaccgctc acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg
     3901 caattaattt aatgctcatc aactattctc catcgccgtc tgcgttttgg tatcgggttt
     3961 ggcgacggct ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc
     4021 actacgtaat ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc
     4081 cagataaccg gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct
     4141 ggcggcagaa atggtcagcg ggaagcccag cgaggcaccc agtccccaaa gtacaacaga
     4201 cactcctgcg acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat
     4261 tagtgcactg gcccgaacca cggcaacgcg actgtaactg tcgatgaacc aaccgccggt
     4321 gaagcgtcca acggtcatcc ccagggtaaa accggcataa atcagtgagc cggaagtggg
     4381 gctaaaaccg tgaccatcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc
     4441 aaaggccatc gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg
     4501 aatgcctttt tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat
     4561 tgcctgaatg gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac
     4621 cgttgccgga acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc
     4681 caggctataa aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc
     4741 ggcaccttca acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag
     4801 accaacggca aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc
     4861 gatcaatgcg caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa
     4921 ccacgccgag cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc
     4981 catttcagcg atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga
     5041 cgccattaac aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa
     5101 tgcattacgt gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac
     5161 attagcaagg ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg
     5221 ctaacgatcc gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt
     5281 acggaataca tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat
     5341 cgatgaccta ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta
     5401 ctgagattat gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg
     5461 catgccaggc tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg
     5521 agctgatgta ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc
     5581 aaaactggat gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc
     5641 gcgcgcttga tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc
     5701 tatcgcgcga ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta
     5761 gataacaaaa aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat
     5821 ggggacatca aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg
     5881 cggttgtgca aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat
     5941 accatgacga tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc
     6001 gcatagccca gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc
     6061 gcgcaggtgc gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca
     6121 taagcaccga acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc
     6181 aacatctgac cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca
     6241 acgcctgcca taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga
     6301 atgtaaccga aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac
     6361 aacccggcag cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg
     6421 atcatcagcc caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga
     6481 atcgagataa agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact
     6541 tgtaaaacgt cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta
     6601 cgaatgacgc ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta
     6661 cctacagcgt tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg
     6721 tcacgatcga aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg
     6781 ggatagccta ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt
     6841 gccagaatcc cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc
     6901 agttcacgca gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg
     6961 cggatcaccg gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg
     7021 atttgctggg cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca
     7081 acaatcaaac tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc
     7141 agtgccagtg agagctgcct gctttccatg ccggaatgat gcagtgtatc gccagcaccg
     7201 accagaaccg gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc
     7261 cagccaaaca gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt
     7321 gctaacatta ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct
     7381 tcgaccccga cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg
     7441 ctgaaatgtt gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt
     7501 tggatcgaac caagtcgtaa ctttccgaga catagcccaa gcgcgaggac cacaaataat
     7561 aacagaatgt aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc
     7621 ttgtttacta gtaagctgtt gaaagaaatg ctaatttacg ataatgtgtt ttaccagaat
     7681 tcagggcgca gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta
     7741 gtgtaatcac attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa
     7801 tgcgaagcgg ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca
     7861 gtgtcaggag gaacgagtga aacataaaca acgttgggcg ggggcaatct gctgttttgt
     7921 cctcttcatt gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc
     7981 cgggcatcct gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt
     8041 ttcacagcgt agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc
     8101 aatgctcatt atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg
     8161 gttactaagc gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag
     8221 tttgtttaaa ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga
     8281 cgatcaggcg tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg
     8341 ttgctgatcg acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag
     8401 atcttcttta gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg
     8461 ttcattgctc aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg
     8521 accaaaaata acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg
     8581 ctaaaatgta ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac
     8641 tgttaaacac aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt
     8701 atgcgcgcga tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg
     8761 ctactggcgg tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct
     8821 cggccagaag tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg
     8881 gtggtggtta ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ctgtccaccg
     8941 caatattcac gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg
     9001 ccatcgcgcc attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat
     9061 tattaacagc gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg
     9121 cattaccgac aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt
     9181 agcgcaagcg gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg
     9241 tccggatgct cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat
     9301 gatggcgcag aatgcattaa ccgcagcgga atcgctggga ttgggcgggg tatatatcgg
     9361 cggcctgcgc aataatattg aagcggtgac gaaactgctg aaattaccgc agcatgttct
     9421 gccgctgttt gggctgtgcc ttggctggcc tgcggataat ccggatctca agccgcgttt
     9481 accggcctcc attttggtgc atgaaaacag ctatcaaccg ctggataaag acgcactggc
     9541 gcagtatgac gagcaactgg cggaatatta cctcacccgt ggcagcaata atcgctggga
     9601 tacctggagc gatcatatcc gccgaacaat cattaaagaa agccgcccat ttattctgga
     9661 ttatttgcac aaacagggtt gggcgacgcg ctaaaaccgc cacgtcgatg tatgatacgc
     9721 gggcttttga ccaggtctga cagagaggtg cagggtgaaa attgccatat tgtcccggga
     9781 tggaacgctc tattcgtgta agcggctgcg tgaagccgct atacagcgcg gtcacctggt
     9841 tgaaattctt gatccgcttt cttgctacat gaacataaat cctgcggcgt cttctattta
     9901 ctacaaaggc cgcaagttac cccattttga cgcagtgatc ccgcgtattg gcaccgccat
     9961 taccttttat gggacggcgg cactgcgcca gttcgagatg ctggggagct atccgctcaa
    10021 tgagtcggtc gccattgccc gggcgcgtga caaattgcgt tccatgcaac tgctggcgcg
    10081 tcagggcatc gacctgcctg tcacgggcat tgcgcattcg ccggatgata ccagcgattt
    10141 aatcgacatg gtcggtggtg cgccgctggt ggtcaagttg gttgaaggca cgcagggaat
    10201 tggcgtcgtg ctggcggaga ctcgtcaggc ggcggaaagc gtgattgacg ctttccgcgg
    10261 tctgaacgcg catattctgg tgcaggaata tatcaaagag gcgcaagggt gcgatatccg
    10321 ctgtctggtt gttggtgatg aagtggtcgc tgcgattgaa cggcgggcga aagagggcta
    10381 ttttcgttcc aatttgcatc gtggcggcgc ggcaagcgtc gccagtatca caccacagga
    10441 gcgtgaaatc gcgataaaag ccgcgcgaac gatggcgctg gacgttgctg gtgtggatat
    10501 tctgcgtgct aatcgcgggc cgttggtgat ggaagtgaat gcgtcgccgg ggctggaagg
    10561 aatagaaaaa accaccggta tcgatatcgc gggtaaaatg atccgctgga tcgaacgcca
    10621 cgctacgaca gaatattgcc tgaaaacggg tggttagtcg caatcacatt actgatcatg
    10681 gttttgcctg cgctttttgc gtaagctgtg ccggtctttt tatcgaaaga ggttgtacaa
    10741 aattatgaca tcgctggtcg ttcctggtct ggatacgctg cgtcaatggc tcgatgacct
    10801 ggggatgagt ttttttgaat gtgataactg tcaggctctg catctgcccc atatgcagaa
    10861 tttcgacggt gtctttgatg ccaaaatcga tctgatcgat aacacgatcc tgttttctgc
    10921 catggcggaa gtccgacctt cagccgtatt gccgctggcg gcggatttat ctgccatcaa
    10981 tgccagttcg ctgaccgtga aagcatttct tgatatgcag gatgataatc tgccaaagct
    11041 ggtggtttgc cagtctttat ccgttatgca gggcgtaacc tatgagcagt ttgcatggtt
    11101 cgtgcgtcag agcgaagagc agatttcgat ggtcattctt gaagctaatg cccatcaact
    11161 gctgttaccg actgacgatg aagggcaaaa caacgttacc gaaaactatt ttctccactg
    11221 ataattcctt tcgagcacgc agtcgctgcc gcagtgactg cgcgctgcaa aattatctgc
    11281 tgtttttaac cttttcttaa agattatttc acttctcttg tgtcgatttg gctttatcac
    11341 atagagcaaa tacgcataaa aatttgttaa ataccgtttt ttaatccgag ctatagtctc
    11401 aaaccctggc taaagttatt cttgcgatgc ttctatatag cgagcagtgc tggccgggag
    11461 agagttctct tttcttacac cgcgccgata aaaaaatatg cacgtttatt gcatatcttt
    11521 cagtgtgaca acttttgttc gtttgttaac gaactttcag aaggaaagag atgtgaccgc
    11581 cttaaataaa aaatggctat cgggtctggt tgcgggtgct ctgatggccg tctctgtcgg
    11641 cacgctcgcg gctgaacaaa aaacactcca catttataac tggtctgatt atatcgcccc
    11701 tgacacggtg gccaattttg aaaaagaaac cggtattaaa gtcgtctacg atgttttcga
    11761 ctctaacgaa gtactggaag gcaaattaat ggcgggtagc accggctttg acttggtggt
    11821 tccatctgcc agctttctag aacgccagtt gactgcggga gttttccagc cgctggacaa
    11881 aagcaaattg ccggagtgga agaatctcga cccggagctg ctgaagctgg tcgccaaaca
    11941 cgatcccgac aataaatttg ctatgcccta tatgtgggcg acgaccggga ttggctataa
    12001 cgttgataaa gttaaagctg tgctgggcga aaacgcgccc gtcgatagct gggacttgat
    12061 cctcaaacct gaaaatctgg aaaaactgaa aagctgcggt gtctctttcc tggatgcgcc
    12121 agaagaagtt tttgctaccg tgttgaatta tctcggcaaa gatcccaaca gcactaaagc
    12181 ggatgattac accggaccgg caaccgatct gctgttaaag ctgcgcccga acattcgtta
    12241 tttccattca tctcaataca ttaacgacct ggcaaacggc gatatttgcg tcgctatcgg
    12301 ctgggcaggt gatgtctggc aggcgtcaaa ccgcgcgaag gaagcgaaga atggagtgaa
    12361 tgtctcgttc tcgattccaa aagaaggggc gatggcgttc tttgatgtat tcgccatgcc
    12421 agcggatgcc aaaaacaaag acgaagccta tcagttcctg aattacctgc tgcgcccgga
    12481 tgtagtagcg catatttccg accatgtgtt ctatgtcaac gccaataaag cagccacgcc
    12541 gctggtgagt gcggaagtcc gtgagaaccc gggtatttat ccgcctgcgg atgttcgtgc
    12601 gaagctgttc actctgaaag tgcaggaccc gaaaatcgac cgtgtgcgca cccgcgcgtg
    12661 gaccaaagtg aagagcggaa aataatccgc agccttagat gccgggcggg cgcaccacac
    12721 ccgccggcaa ttcgcaccat catggtgcgc ttgcacacat tcaatgccgg agagcagccg
    12781 tgaatgacgc tatccctcgc ccgcaggcga aaacccgtaa ggcgctgacg ccgctattag
    12841 aaatccgcaa cctcaccaaa tcctacgatg gtcaacatgc ggtggatgat gtcagcctga
    12901 ccatctacaa aggtgaaatc ttcgcgctgc tgggcgcatc cggctgtggc aagtccacgc
    12961 tgctgcgtat gctggcaggt ttcgaacaac cttctgccgg acagataatg cttgatggcg
    13021 tcgatttgtc acaggttccg ccttacctgc gccccatcaa tatgatgttt cagtcttacg
    13081 cgctgtttcc gcatatgacc gtggaacaga acatcgcttt tggcctgaaa caggacaaac
    13141 taccgaaagc ggaaatttcc agtcgggtca atgagatgct cggcctggtg cacatgcagg
    13201 agttcgccaa acgtaaaccg catcagcttt ctggtggtca gcgacagcgt gtggccctgg
    13261 cccgaagcct tgccaagcgc ccgaaactat tactgctcga cgagccgatg ggcgcgctgg
    13321 ataaaaagct gcgtgaccgg atgcagcttg aagtggtgga tattctggag cgcgtcggtg
    13381 tgacttgtgt gatggtcacc cacgatcagg aagaggcgat gaccatggcg gggcgcatcg
    13441 ccattatgaa tcgtgggaaa tttgtccaga ttggcgaacc ggaagagatc tacgagcatc
    13501 cgactacccg ctatagcgcc gaatttattg gctcggtaaa cgtctttgaa ggcgtactca
    13561 aagagcgtca ggaagatggc ttggtgcttg attcgccggg gctggtgcat ccactgaaag
    13621 tcgacgcgga tgcctcggtg gtcgataacg tgccggtaca tgtggcgctg cgcccggaaa
    13681 aaatcatgct ttgcgaagag ccgcccgcca atggttgtaa cttcgcggtg ggggaggtga
    13741 tacacattgc ctatctcggc gatctttcgg tgtatcacgt tcgtctgaaa agtgggcaga
    13801 tgattagtgc ccagctacaa aacgcccatc gtcatcgtaa agggttaccg acctggggcg
    13861 acgaagtgcg tttgtgctgg gaagtggaca gctgtgtggt gctgacggtt taaggagcaa
    13921 agatgaatac acttgaacct gctgcccagt cgaaaccgcc gggcggattt aagctgtggt
    13981 tgtcgcagct gcaaatgaag catgggcgca aactggtcat tgcgttgcca tatatctggt
    14041 tgatcttgct gtttctgctg ccatttctga ttgtctttaa aataagcctg gcggagatgg
    14101 cgcgcgctat tccaccttat accgaactga tggagtgggc tgacgggcaa ctgtccatca
    14161 ctcttaatct cggtaatttt ctgcaactga ccgacgatcc gctctatttc gatgcttatc
    14221 tccagtcgct acaggtggct gcgatttcga cattttgctg tttactgatc ggctatccgc
    14281 tggcgtgggc ggtggcgcac agtaagcctt cgacccgtaa tattttatta ctactggtga
    14341 tcctgccgtc gtggacctcg tttctgatcc gcgtttatgc ttggatggga atattaaaaa
    14401 acaacggtgt gctgaataat tttctgctgt ggctgggggt tatcgatcaa ccgctgacca
    14461 ttcttcatac caatctggca gtttatattg gcattgttta cgcctacgtg ccgtttatgg
    14521 tgctgccgat ttataccgcg ttgattcgta ttgattattc gctggtggaa gcagcgctgg
    14581 atctcggtgc acgaccgctg aaaacgttct ttactgtgat cgtgccgctg actaaaggtg
    14641 ggattattgc cggatcgatg ctggtgttta tcccggctgt gggcgagttt gtgatcccgg
    14701 aactgctcgg tggcccggac agcatcatga tcgggcgcgt gctatggcaa gagtttttca
    14761 ataaccgcga ctggccggtg gcctcggcgg tagcgatcat catgttgctg ctgctaattg
    14821 tgccgataat gtggttccac aaacaccagc aaaaaagcgt gggagaacac ggatgaataa
    14881 tttaccggta gttcgttcgc cctggcggat tgtgattttg ctgctgggct ttacctttct
    14941 ctacgcgcca atgctgatgc tggttatcta ttcgtttaac agctcgaagc tggtgacggt
    15001 gtggggcggc tggtcaacgc gctggtatgg tgagttattg cgcgatgacg cgatgatgag
    15061 tgcggttggt tcaagcctga caattgctgc ctgtgcggca acggcggcgg cgatcctcgg
    15121 gactattgcg gcggtggtgc tggtgcgctt tggcaggttt cgcggatcaa atggctttgc
    15181 ctttatgatc accgcgccgc tagtaatgcc agatgtcatc acgggcttgt cgctgttgtt
    15241 attattcgtc gcgcttgctc atgccattgg ctggcctgcg gaccgcggta tgcttaccat
    15301 ctggctggcg catgtcactt tctgtacggc ttatgtggcg gtcgttattt cgtcgcgtct
    15361 gcgggaactg gatcgctcga tagaagaagc cgcgatggat ctcggtgcga cgccgctgaa
    15421 agtgtttttc gtcattacgc taccgatgat catgcccgcg atcatttctg gctggttact
    15481 ggcttttact ttgtcgcttg atgatctggt gatcgccagc tttgtttccg ggccgggagc
    15541 caccacgtta ccgatgctgg tcttttccag cgtgcggatg ggggtgaatc cggaaatcaa
    15601 cgccctggca acattgatcc tcggcgcggt cggaattgtc ggatttatcg cctggtatct
    15661 gatggcgcgc gcagaaaaac agcggatacg cgacatccaa cgtgcaagac gtggctgaag
    15721 acactaaaat ttgccaacct ggctacataa tgccgcgcat gtcgcggcat tgttttcatg
    15781 gaagacgaaa cgttgggatt ttttaagaaa acatcttcat ctcatgctcg cctgaatgtg
    15841 cctgcgctgg tgcaggtggc ggcgctcgcc attattatga tccgtggcct cgacgtgctg
    15901 atgattttca atacgctggg cgtgcgcggt attggcgagt tcattcatcg cagcgtacaa
    15961 acctggagtt taacgctggt ctttttaagc agtctggtgc tggttttcat tgagatctgg
    16021 tgtgcgtttt cactggtgaa agggcgtcgc tgggcgcgct ggctatatct gctgacacaa
    16081 atcaccgccg caagttactt gtgggcggct tcgctggggt acggttatcc ggagctgttc
    16141 agcattcccg gtgaatcaaa acgtgaaatc ttccatagcc tgatgctgca gaagctgccg
    16201 gatatgctca tcctgatgct gctgttcgtt ccctcgacca gtcggcggtt cttccagttg
    16261 caataatgtg tataatcgtc gcccctgatg atgtgaaggt caatgtatgc agtgcgcact
    16321 ttacgacgcg ggtcgctgtc gttcctgtca gtggataacg cagccgattc cagagcaact
    16381 ctccgctaaa accgccgatc ttaaaaatct gctcgccgat tttccggttg aggaatggtg
    16441 cgcgccggtg tcaggcccgg aacaagggtt tcgtaataaa gccaaaatgg tggtgagtgg
    16501 tagcgttgaa aaaacactgc tcggtatgct gcatcgcgat ggcacgccgg aagacctttg
    16561 tgactgcccg ctttatccag cctcatttgc gcccgttttt gcggcgctaa aaccgtttat
    16621 cgcccgagcg gggttaacgc cctacaacgt ggcgcgtaag cgtggcgaac tgaaatacat
    16681 tctgctgact gaaagccaga gtgatggcgg catgatgctg cgctttgtac tgcgttctga
    16741 taccaagctg gcgcaactgc gtaaggcgct gccgtggtta cacgaacaac taccgcagct
    16801 gaaagttatt accgtcaata ttcagccggt acatatggcg attatggaag gggagacgga
    16861 gatctacctg accgaacaac aggcactggc ggagcgtttt aatgacgtac cgctgtggat
    16921 ccgtccgcaa agtttcttcc agactaatcc ggcggtcgcc agccagttgt acgccaccgc
    16981 gcgcgactgg gtacgacagc tgccggttaa acatatgtgg gatctgttct gcggtgtggg
    17041 gggctttggt ttacactgcg cgacgcctga catgcagtta accgggatcg aaattgcgcc
    17101 agaggccatt gcctgtgcaa agcagtcagc cgctgaactg ggcttaactc gtttacaatt
    17161 tcaggcgctg gactccactc agtttgccac tgctcagggg gaggtgccgg agctggtgct
    17221 ggttaacccg ccgcgccgcg gcattggtaa accgctgtgt gattatcttt caacgatggc
    17281 accgcgtttt attatctact ccagctgtaa cgcccaaacc atggcgaaag atatccgcga
    17341 actgccaggt taccgcatcg aacgggtaca gctttttgat atgttcccgc acaccgcgca
    17401 ctatgaagtg ttgacgctgc tggtgaagca ataaaaaagc cacaggtgcg gcttcagatt
    17461 gctgatcagg tgcgctttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    17521 aatcatgcta aatcaataca ttgcagagtt gatgtgggcc ggataagcgc agcgcatcag
    17581 gcaatcttac ttttgtcatc agtctcaatt actgcggaaa ccactggtca ttgattttct
    17641 gatatgtacc gtcagcttta attgctgcca gcgcgttatt cagtttttcc agcagagctt
    17701 tgttatccgg acgtacagcg atacccaggc cggtgccgaa gtattgcgga tcggtcactt
    17761 tttcagtcgc aacgcccagt tgcggattgg ttttcagcca ttcgtttacc accgcggtgt
    17821 caccaaatac cccatcaata cgaccatttt tcagatcgat aaaggcattc tgataactgt
    17881 cataggagac agttttcact tccgggtgct gatcctgaat atatttctgg tgcgtcgtac
    17941 cgttttccat cccaatgcgt ttgcctttca ggtcggcaaa cgttttgtag gtatcttttt
    18001 tggaaatcac gacggctgag ttttcatagt agggtgtggt aaacgaaacc tgcttgctac
    18061 gctccggggt gatatccata ccggagatta cggcgtcata ttttctgaat ttcagtgacg
    18121 ggatcaggct gtcgaacgcg tgattagtaa aagtacattc tgcctgcatt tgtttgcaca
    18181 aggcttttgc cagatcgata tcaaagccga caatctcatt attagcacct atagattcaa
    18241 agggtggata ggtggctgaa acgccaaaat tgattttctc tgcggcagaa gcaccgaaag
    18301 taaaggaagc aagtaaagcg gcaagaacta actttttcat gatggaactc ccgtctgtca
    18361 atcttatgat ttttggccgt gtctgcggca tgggataaca atgccatcaa gtgaatttat
    18421 atgcaataaa catgattaaa taatttaaat gaaataaaaa aagacggata acttagtgag
    18481 ttgtccgtct tcattataag aatttatgca ctatgtaggc cggataaggc gtccacgccg
    18541 catccggcac aggcaccgtg ctgatgtctg atgcgacgct ggcgcgtctt atcagaccta
    18601 caaacccccc cggcgaatgt acgcagctac attaattccg ccgttcgaat gccagtgctt
    18661 tgcgctcgat cagacgcatc atcagcgtca gcaggccgtt gacgaccaag taaataatcc
    18721 ctgccgcacc gaacaccatt acatcgtagg tgcgtccgta caacaactgg ctgtatccca
    18781 tcacttccat cagcgtaatg gtgtaagcca gagaggtact tttgaacacc agcaccactt
    18841 cgttggaata agaagagagc gagcgtttaa aggcatacgg cagcaggatc gccagcgtat
    18901 cttttttgct cattcccagg gcgctacagg actgccactg accttctggg atcgcacgaa
    18961 ttgcaccgta aaacagctgc gtggtatacg ccgcactgtt cagcgataac gcaatcagcg
    19021 cacataacca cggttctgac aacaaatgcc acagtgccgg atactcctgc aacgtcggaa
    19081 actggcccgg cccgtaataa atcaggaaga tctgcaccag cagcggcgta ccggtaaaca
    19141 gcgtgatata accccgcacc agccacacca gcaccggcgt tttcagcgtc aggatgatgg
    19201 taaaaatcaa tgccagaatc agtgccacaa tcagcgaggc aacggttagc gtcaggctgg
    19261 tgtgcagccc tttcatcagc tcgggtaaat actcaaacat tagctgggcc tccgctcaaa
    19321 acgtgtcgcg cgcaggtcaa tgcgtttgag aatgtactga ctgagcaggg tgatcaccag
    19381 gtaaatcgcc gccgccacaa tgtaccaggt aaatggttcc tgggtacgag tagcaatgct
    19441 tttggtttgt aacattaaat cattcacact aatcaaactg accagcgcgg tatctttcag
    19501 cagcaccagc cactggttac cgaggccagg cagcgcatga cgccacatct gcggcatcac
    19561 cagacggaaa aagatagccg atttcgacag ccccagcgcc tgaccagatt cccactggcc
    19621 caccggtaca gctttcaacg cgccgcgcag cgtttgcgag gcataggcgg catacagcag
    19681 tgacagagcg atgacaccac aaaggaacgg actcacgtcg aagttctcaa tgtccatctg
    19741 caccgggatc tgcacgaacc caagattgat agtgaagcca tccgaaagcg tcagcagtaa
    19801 ctgcgaggag ccaaaataga taaacagcac taccagaatt tctggcaggc cacgcagaat
    19861 ggttaccagc gctgaacctg cccacgcgac aggacgccat ttcgctgact cccataccgc
    19921 aaagaacatc gccagcgcca gcccgacaat caatgcacaa acggcaaggc cgacggtcat
    19981 cccggcggcg cttgctaaag gaaaaaattc attcatcagg aattacttct ggaaccattt
    20041 gttgtagatg gtttcgtaag tgccatcttt cttcactttt tccagcgcag tgttgagttt
    20101 ctgctgcagt tcagtgtttc cctgacgtac cgcgatgccg aggccagtac cgaaataatc
    20161 tttatcggtc actttatcgc ccactgccgc cagttttggg ttatctttca gccactcagt
    20221 gaccactgcg gtgtcaccaa atacgctgtc gatacgcccg ttttgcagat ccagttttgc
    20281 gttctggtag ctgtcatacg gaacggtggt gatttccggg tgcttatcca taatgaactt
    20341 ctggtgtgtc gtcccgttct gaacgccgac ttttttgcct ttcagctgat caacactggt
    20401 gtatttgcct tgctgaccca caaacagggc agagttgtca tagtacgggg tagtaaacag
    20461 tacctgcttt tcacgctccg gagtgatatc catgcccgcc atcactgctt ctacgcgacg
    20521 gaatttcagg cttgggatca ggctgtcaaa cgcctggtta gagaaagtac aggttgcatc
    20581 aatctcttta cacagtgcct gcgccaggtc gacgtcaaaa ccaacgatct ggttgtttgc
    20641 atcaatcgat tcaaacggag gatagggggc ttcggtagca aaacgaatgg tttcggcagc
    20701 tgtggcggaa agactaaaac ctgcaattaa cgcggcaatc agaacttttt tcattgttgt
    20761 tatcccgaat cttagtgaga gagatagttt ttaaatgctt cggtttgcgg ctcggtaaag
    20821 cagctcgcgt cgccttgttc tacgatatga ccattttcca tatacaccac tcggctggcg
    20881 gttttacgcg ccacttcaac ttcgtgggtg acgatcacct gggtaatatt cgtttctgcc
    20941 agctcacgaa tgatgctgac gatttgtgcc gtaatttccg ggtccagtgc ggcggtcggt
    21001 tcatcgaaca gcagtacctg cggttccatc atcaacgcac gggcaatagc aacacgctgc
    21061 tgctgaccac cagaaagatg cagcgggtaa cgatcgctat aaggtttgag acgcagacgt
    21121 tccagcagtt tttctgcacg ggccagcgcc tgatctttac tcaaccccag tacacggcag
    21181 ggcgcttcaa tcaggttttg ctgcacggtc agatgcggcc acaggttgta ttgctgaaac
    21241 accatgccaa cgttacgacg caaatcgcga atcgctttgt cagagggtgt tttggtgaaa
    21301 tcgaaatggt tgcctgcaat gttgagcgta ccggagcgcg gcatctcaag cagattgagt
    21361 acacgcagca gcgagctttt acccgcgccg ctggggccaa gtaacaccag cgtttcgccc
    21421 tgtgggcaat ccagcgtgat atcgaacagc gcctgatgcg cgccgtagaa gcaattaatg
    21481 ccgtttaatt gaatactcat tgacactcgt atactggcag tctgatagct attgaggtcg
    21541 aagatagtac ctttaacaga ataattatgc aatatttatg ctttaaaagt taaaagcaaa
    21601 gcgcattatt caataaacat agcacaaaat aacgggggcg gtggtcggtg agcataaatg
    21661 tcggcattcc tcacgaaatg ccggataatt tacggggttt attggttgat caaggcgtta
    21721 gcgattctcg atggactgac ggagcgtacc cgccgtggca tgaacgctac cgcctaagta
    21781 gcgcacatcg tcgatgaccc aacactggcc ttcctgaatc attaacactt catcctgcca
    21841 gccctggtca ccctgtttga gatccacccg caatggaatg ttacgggcat cacgattagg
    21901 gatagtcgat gcactggcaa cgtgggcgct gtctggcaag gtggttcgac tggagaacgg
    21961 atcgctgctt aacaattcac gatgactatt atcgcggctg gcatcgctaa gttgggttgc
    22021 cagtttgtca ctcaaatagg ggcgcagggc ggtaatgtca ttgctgcgat gttgaatgcg
    22081 gtagtcgtag aattgttgag ccacactgtc aggccctccc tggacacagg aaccggtgcg
    22141 ggggccgtta tctttataag ctggcgtgac ggtggtacag gcactcagca gcagtgcaca
    22201 tggaataagt agagatagtg aacggtagcg cataatcatt tccttattag cttgctgtct
    22261 gcgtcacact acgaccaaac gctatcagat tatttaatac attgttttta atacaattct
    22321 gaaacaatct gaacacctga ttgggtttga gtccattgct ggcgacccac tcaaggttgc
    22381 tcgtagctga cttacttaaa ttacccaaac aatagacagt gtaaaccatt tgtgaggttg
    22441 agcgtgttat atggctgaaa tgatcattta aggtaaatat gatagttctg aaatggtggt
    22501 ataaatcctc ctgtagaggt taagagcatc tgcaagaagc cagtttcaca tttataacgc
    22561 gtgataaatg aatgcggtta ttgtctaatc attacaaaat ttttcacatg cgataagaga
    22621 tcctgcgatt aagatgattg ccaaaattac gttgtatttt tgaaaatgag cgaaatttga
    22681 tagtcccagc tttgactctc gataatatta tttatgctac aattgtgggt aaagaattta
    22741 agctaatgaa tttgagtgaa gaataaatta aattgcttca gtatttcgtt aattaatata
    22801 aatacaaaag gcgttgtgct tttataatta taaagcgcga ggagttcggt ggattatcgt
    22861 ttttcttggg tgatttttaa tatgataaac aatatatctg atcaagcatc atcatttcca
    22921 ggcacacaac taaatcaaag tgataatttc ctggattcac tgagggaatt tttcgcgata
    22981 ttaaatccca gtcgtaaggg tgagttgtct acatgggata ccatctactt gcatctgata
    23041 ttagcaataa atgctgacag tgatctcata aaaaatgatg tgttgctcgc ggaaaatatt
    23101 ccatcagcga attaccaatt taatacattt tttagcaata ctttcgagat tgatgttaaa
    23161 aaatatctgg gtaaatcaga agataacgaa gttgaaataa aagcgggtaa tgaaagaatc
    23221 tctattggaa tacgtaatgt atcgaatgga aaacttgaaa gacaacagtt tcttttccca
    23281 ctggactatg agaataaatt acaagaacaa ttagataagt attttacgat tgaatcgcac
    23341 ccattattat acaggtatac cattggtagt aagattgcga atgtgatttt tgaaaagtta
    23401 tacagtcgta ttgactttaa caaagaacaa tatatatcct ttataaagga cgcatttatt
    23461 catttttatg attatagcag gcgatatgct attagtgaga atatcgataa agacgctgta
    23521 acaaacaata tcgcattgat gagcactttt tatgacagtg ataatacgtc aggtgaggtt
    23581 ttaaataatg attttacaga agaagagtca tttgaaactg cgttagatgt agagcatgca
    23641 atagtgcttg gatttgctga tgataatttt gaaacaaaac ctgttcatta tcaggattta
    23701 ttaacacgat ttagtgcctt tcaagatacc gtgtttaatc tgtttcctga aatgcactct
    23761 tcgcattacc acgatatttg tagcgtaagt gtggatatga caaagggaac gcaatgcatg
    23821 attcacttga tggtgaatga agaagttttc atgtctctgc ctgttccagt tgccacgatg
    23881 gtacgtgaag atgcaagtaa tttagtcaat cttaaaacac tactcaatga tggttgtttc
    23941 attaaatata gtcattttaa tgacgtggca ttaataaagc aaaacataag taatctctat
    24001 ttatcacaca ctgttgtgaa tgaaagcata ttgaaaaaat gttgttttga aaacggcagt
    24061 ttaggtgatg ttaaaataac aaatagcaat gtaataaaca gtgcattcaa aaatatttcg
    24121 tttagatctg ttaaaattaa taacgtaaat actcattctt taaaatttat taactgcaat
    24181 tttttcaatg ttgatatgat aagagttaat ctcagcaaat gtctttttca tgaatgtagt
    24241 atgcatggtg tcaagataaa accctggttg cctgtaaaat ggacaaaaga attaataagt
    24301 gattatttgt atggctgttt actatctctg tactctatct gtgcaaggga tatatataat
    24361 atgaacgccg ggaataacgt gaaggttgct gcggatgctt tccttgaaat tattttctct
    24421 cttaaaaata aatattgtat taaactatta agcgctcagg atcgggcgtt tatatacgag
    24481 tttgcaagaa tgatcttcgc ttatattaat gacaaaagca ttgagatctt attactttct
    24541 tgttttgcgg cggctgacca aaaagcaatc cagcggtata gacctcagtc acaagatggg
    24601 gaagacttta ggagtcattt acaatataag ttgcctcttt ccgctcatta atgttgttgg
    24661 tgcaacataa taagacaggt aagcggtacc ctataatgaa accaatatcg ggttatgcaa
    24721 cggataatcg taaagactta agtgggtgtt tttgacacaa tgagatgatt tcctaaactg
    24781 atcaaaacct tcttcccaac atggcatatc agtccgataa tgctttagag atcgaaggct
    24841 taacaaacaa ggagatcgat catgcaattt tctacaaccc caactctgga aggcctgacc
    24901 atcgttgaat attgcggtgt ggtgaccggc gaagcgattt taggtgccaa tattttccgt
    24961 gatttctttg ccggtatccg cgatatcgtt ggcggacgtt ccggtgcgta tgaaaaagaa
    25021 ctgcgtaaag cacgggagat cgcctttgag gaattaggct cccaggcgcg ggcgctgggg
    25081 gccgatgccg tagttggtat tgatatcgac tacgaaacgg tcgggcaaaa cggcagtatg
    25141 ctgatggtta gcgtcagcgg tacggcggtg aaaacgcgtc gatgagaaga gtcttctggc
    25201 tggtcgctgc cgctctgtta ctggcagggt gtgcaggcga aaaaggcatt gtcgagaaag
    25261 agggatatca gcttgatacc cgacaccagg cgcaggcggc gtatccgcgc attaaagtgc
    25321 tggtgatcca ctacaccgca gatgattttg atagctcgct ggcgacactg actgataagc
    25381 aggtcagctc gcattatctg atccctgcgg tgccaccgcg atacaacggt aaaccgcgca
    25441 tctggcaact ggtgccggaa caagaactgg cctggcatgc ggggattagc gcctggcgcg
    25501 gggcaacgcg ccttaacgac acctctattg gcattgagct ggaaaaccgt ggctggcaaa
    25561 aatcatccgg agtgaaatat tttgccccgt ttgaacaggc acagattcag gcgcttattc
    25621 cgctggcgaa agatattatt gcccgttatc acatcaagcc ggaaaacgta gtggcacatg
    25681 cggatatcgc accgcagcgc aaagacgatc cggggccatt atttccctgg cagcaactgg
    25741 cgcagcaggg gattggtgcc tggccggatg cgcagcgggt gaacttttac cttgccgggc
    25801 gcgcgccgca cacaccggta gataccgcgt cattgctgga gcttttggcg cgctacggtt
    25861 atgacgtaaa acctgatatg acaccgcgcg agcagcggcg cgtgattatg gcattccaga
    25921 tgcatttccg cccgacgtta tataacggcg aagcggatgc agaaactcag gcgattgccg
    25981 aagcattgct ggagaaatac gggcaggatt agcgcggcag ttttccgtgg tcgcgtagcc
    26041 aggcggcagt tttctcgatg ccttcatcca gggtgatgac cggctgataa cctaactctt
    26101 cctgcgcacg cgtaatatcc agcgtaaagt caaaattcaa cttggaggcg ccgtagtggg
    26161 tcagcggcgg ctcttttgct gacttgcggc ctaaacgctc catgctgcgg gcgatcatat
    26221 ccagcatcgg gtaagggacg gaacgaatac gacagtcaat attcaactcg tcgatcagct
    26281 tctgcacgat gctgcgcagt gtgcgatgct cgccgttggt gatgttgtac acacgcccgg
    26341 aaggtagctt atcgcaggct tcctggcttg ccagccacat tgcgtgcacg gcattttcat
    26401 agtaggtcat atcgaccagc gcactgccgc catgcggtaa cagaatactg ccgtagtggt
    26461 gcatcatatg cgccagacgg ggaataaaga ctttatcgtg cggtccgaac agactttgtg
    26521 ggcgcagaat agtaaagcgc gtttgtggat tcgcctgcga aagcatattg atcacttctt
    26581 cgctggctgc tttgctgcgg gcaaactcgt tggcgaagcg gtgagggcga aaatcttctt
    26641 taatattgcg atggtggtga taatcgaagt acagggaggg ggaagagata tgaataaagt
    26701 tacgcacacc ccaggcgaca gcccattcac ccaggcggcg agtggcgcga acgttagcca
    26761 gatcgaaagc ctgttgtgtc ccccagggtg aggtaaagct ggagcagtgc cacagcgtat
    26821 caatgcccgc gagcatcact ttagcttgtg atgaaaccag ctcggtcaga tccgtcggaa
    26881 caaactctgc gcccattttt tccagcaatt tacccattgc ttcgttgcga ccgctcgcac
    26941 gcacgctgat gcctttctgg cataaaaact ctaccgcgtt tcgacctaag ccgctggtgg
    27001 cgccggtaac cagtaccttc atatcaatcc actgttgttg agaaaatgac gtgcgcattc
    27061 ttccgtgatt tcccccatga tgcaatggga aacatgaaag aataacgcag gttttgtcga
    27121 ttaatctgtg ctttgttctg ccagtctggc gatttgtttt gccattccgc gaaaaataaa
    27181 cagatgcgcg gggatcatca ataaccagta aaacagcccc ggcataccgt gcggatgcca
    27241 gaaagcgcgg acatcgatag tacgatagtc acctttatct tccagggtaa aacacagtcg
    27301 tcccagcccc ggcgctttca tgccaaataa caacgtaagt tgctttttcg gttcaacgac
    27361 aatcactttc cagctatcca ccgcatcgcc ggtctgtaaa tattcgcgct ccgggcggcc
    27421 tttcgccaga ttatgaccga tcgcgcggtc catcaacgcc cgtgtctgcc acaaaatatt
    27481 gccaaagaaa taacgctctt taccgccgat ttggttcacc accagccata aagccgcaag
    27541 gctggcggac gttttaacgg taaaccccgc ctgtttggcg aaataaccgt actccggtcg
    27601 ccagcgggca aaggcctgag cgtcgtagcc ccagtcgctg gagttgacca gtttttcttc
    27661 ctctttcaac gtgctacgta ccgcgtcatc gaaagcgatc agccgttgtg ggatgagtgc
    27721 acgtagcgcg gtatcatctg ccagcagatc gtgtttcagc ccctgaatca acgccctggc
    27781 ggtggtgggc ggtacggaag taatcacatt gagaaaccac accgaaatcc agcgggtggg
    27841 gagggggatg gggatcaacc agcggcgctt accgctcacc gccataaaat gttcaaactg
    27901 ttgctgataa ctgagcacct ctggtccggc ggcttcgaag atgcggtgtt cgttggctgg
    27961 atggtctaac agcgccacca gatagtgcag caagttttcc agcgcgatgg gcgtggtgcg
    28021 tgaacgtacc cagcgtggcg gcgttaacac tggcaggttg tagaccatat cgcgcatgac
    28081 ttcgaacgcc gctgaacctg cgccaacgat aattccggcc cgaagttcgg tcactggtac
    28141 attcgcttca cgaagaatgt ccgccgtagc ctgacgagct cgcagatgat ccgactgctc
    28201 atgtggcggg gcctgcaacg aactgagaaa gattaattgc ttaactggta cttcacgtag
    28261 cgcatcgcgg acgttgagag ccagctggcg ctcctgagcg ataaaatcgc cgccttcgcc
    28321 catgctgtgc accagaaaat agacggtatc gatatcctgc aacagggccg gaaggttatc
    28381 cggccagctg agatcgactt tatggcaact gacgtttgcc agttgcagct ttgcaagcct
    28441 gtcgacatga cgtgccgccg ctaggatctg atgcccttgc tggctgagtg tgcgcaccag
    28501 atgctgacca atgtagccac tggcaccgag aactaaaatg cgttgcggca cgtctctctc
    28561 cttaacgcgc caggaatgcg cgccagtggg cggcgacttc cgccagttgt tcgcgcgaga
    28621 cgtcaagatg cgtcaccagg cggacaatcg gcgaggcgtt aatcagcacg tttctcgctt
    28681 tcatgtattc gcctaacgcg gcagcatttt cttccccgac gcgaacaaac agcatattag
    28741 tgtcctgacg catcacatcc gcgcctgctt cacgcagttg ctccgccatc caggcggcgt
    28801 tgtcgtgatc ttcctgcaac cgtgcgacgt tattttccag ggcatacatc ccggcggcag
    28861 ccagaatccc ggactgacgc atcccgccac cggccatttt ccgccagcgg atggcacgtt
    28921 taatgtaatc acgattaccg acgagtaatg aaccgactgg cgtcccaaga cctttcgaca
    28981 ggcaaatggt gaacgaatca caatattgcg tgatctcttt cagttcgcag ccgtaagcca
    29041 ccacggcatt aaagatgcgc gcaccgtcaa catgcagcgc cagattgcgc tcgcgggtaa
    29101 attcccatgc ttctttcagg tattcacgcg gcagcacttt gccgttgtgg gtgttttcca
    29161 gactgagtaa tttggtgcgg gcgaaatgga tatcgtcggg tttgattttc atcgccactt
    29221 tatccagcgg tagcgtgccg tcggcagccg cgtcgatggg ttgcggctga atactgccca
    29281 gcaccgctgc gccaccggct tcaaacagat agttatgcgc ggcctgaccg acaatatact
    29341 cttcgccacg ttcgcagtga ctgagcagag cgaccaggtt ggcctgagtg ccggtaggca
    29401 gaaaaatggc ggcttcttta ccggaaagct ctgctgcata gtcctgcaga gcattaacgg
    29461 tagggtcgtc tccgtaaacg tcgtccccaa ccggggcggc catcatcgct tcgagcatgg
    29521 cgcggctcgg tcgggtaacg gtatcactgc gtaaatcaat catggcatgt ccttattatg
    29581 acgggaaatg ccaccctttt taccttagcc agttcgtttt cgccagttcg atcacttcat
    29641 cgccgcgtcc gctgataatt gcgcgcaaca tatacaggct aaaacctttg gcctgttcga
    29701 gtttgatctg cggtggaatg gctaattctt ctttggcgac caccacatcc accaacaccg
    29761 gaccgtcgat agagaaggcg cgttgcaggg cttcatcaac ttcagaggct ttttctacac
    29821 ggatacccgt aatgccgcac gcttcggcaa tgcgggcaaa gttggtgtcg tgcagctcag
    29881 taccgtctgt caggtaacct ccggccttca tctccatcgc cacaaagccc agcacgctgt
    29941 tattaaagac gataattttc accggcagtt tcatctgcac tactgagagg aaatcgccca
    30001 tcaacatgct aaaaccgcca tcgccgcaca tggcgaccac ctgacgttcc ggctcggttg
    30061 cctgcgcacc caacgcctgc ggcatggcgt tagccatcga accgtggtta aacgaaccta
    30121 acaggcgacg cttgccgttc attttcagat aacgtgccgc ccataccgtt ggcgtgccga
    30181 cgtcacaggt aaagatggcg tcatcggcgg caaaatgact aatttgctgc gccagatatt
    30241 gcgggtgaat ggctttctcg ctcggtttag ctaaatcgtc cagccctttg cgggcatcgc
    30301 ggtaatcttc cagcgcttta tccagaaact tgcgatcggt tttttcttcc accagtggca
    30361 gcaatgcgcg cagggttgat ttgatatcgc cgaccagcgc catatctacc ttgctatgcg
    30421 cgccgatgct ggctggatta atatcaatct gaataatttt ggcatcggtc gggtagaacg
    30481 cacggtaggg gaactgagta ccgagtagca ctaacgtgtc ggcgttcatc atggtatgga
    30541 aacctgacga gaatccgatt aacccggtca ttccaacatc atacggatta tcgtattcga
    30601 catgctcttt accgcgtagg gcatgaacaa taggtgcttt aattttcccg gcaaactcaa
    30661 ctaactcttt atgcgccccc gcgcagccgc tgccacacat cagggcgata ttgctggaat
    30721 aacgcagcag ttgcgccagt ttgcgtaact cttcttcttc cggcatcacg actggctgtg
    30781 gcgcatgata ccagtgcgtg gttgcccctt ctggcgcagg ttttaacgcc acgtcgcccg
    30841 gtaatacaac aacggaaacg ccacggttaa gcaccgcttt gcgcatagca attgccagca
    30901 cttgtgggat ctgctccggg ctggaaacca gctcgcaata gtgactacat tcgcggaata
    30961 gctcttgtgg gtgggtttcc tggaaatagc cgctgccaat ttcgctggag ggaatatgag
    31021 cggcaatcgc cagtaccgga acgttattgc ggtggcaatc gaataggccg ttgatcaagt
    31081 gcaggttgcc tgggccgcac gatccggcac agaccgccag ttctcctgaa agttgtgctt
    31141 cagcgccagc ggcaaacgcc gccacttctt catggcgggt ggacatccac tcgatggtgc
    31201 ccatgcgatt aagactgtcg ctaagaccgt tcagagagtc gcccgtgact ccccagatgc
    31261 gtttcacgcc tgccgattcg agtgttttgg cgatataagc tgcaaccgtt tgtttcatgg
    31321 ttctccatct cctgaatgtg ataacggtaa caagtttagt tcatctgacg gagggggaag
    31381 ggatgggaga gaaaggaggc actaacggtt aaatagcccg atgaaaggaa tatcatcggg
    31441 cataaggcga ttatgcgaga accaaatccc cctgcggatg gcaggagcag gccagtacgt
    31501 aaccttcagc gatttcggcg tcggtcagcg tcattgtact gctcaccgta tattcaccgg
    31561 aaaccacttt tgtcttacag cagccgcaaa cacccgcacg gcaggcagcg acaaccggaa
    31621 cgttattgct ttccagcgcc tccagtagcg tggtgccaac cggggcgtaa aattctcgtg
    31681 ccggttgcag tttggtgaat ttcagaccgc tggtcgccgc ttctgctact ggggtgaaga
    31741 atttctcttt aaagaaacgc gtcacgccga gcgctttcac ttcctgctct acccaatcca
    31801 tatacggagc cgggccgcag gtcatcacgg tacgtgaagc taagtcaggt acatctgcca
    31861 gcagttcgcg agtgaggcga ccagcgataa agccttcggt aacgttattt tctgccacca
    31921 gcgttaccgg atagttacgc cactcatcgg cgaaaataac atcctgcggc gtacgcacgt
    31981 tgtagatcac ccgcacatcg gcctgtggac ggttcttcgc aagccagcga cgcatcgaca
    32041 taatcggcgt gacgccgcag cctgccgcca gcaacaggaa tttatcttct gctttatcgt
    32101 cgcaggtaaa ttcccccatc gcgtccgaaa gccagagata atcaccgcgt tttacatcgc
    32161 gcgtcagcca ctgggagccg acaccgtcat caatccgccg cacggtcagg gtgatatatt
    32221 cactcacgcc tggcgtggag gaaatggtgt aagcacgcag cgtttccgct gagtttcgca
    32281 cgctgaccag tgcatattgc ccggcgcgat atgggtagtg atcgtggcaa atcagggaaa
    32341 tcgtccacac atccggcgtt tcttgcgtaa tgtgatgaac ctgtatccgc cacgggcatt
    32401 gattcgttgg catcgtcatc gacaaactcc ttacgcgttc aacagttgct tcatgtcttc
    32461 ttcaacagtg gtgatagaac gcaggccgaa tttctcgttc agcaccgcca gcaggtctgg
    32521 tgtcaggaaa ccaggtgcag tcgggccggt gacgatattt ttcacgccca gagaaagcag
    32581 cgtcagcaga atgacaatcg ctttctgttc aaaccaggag agcaccagcg acagcggcag
    32641 atcgttcaca ccacagccca gtttctctgc cagagtgaca gccagaataa ttgctgagta
    32701 agcatcgtta cattgacctg catctaccag acgcggcaga ccttcgatat cgccaaactc
    32761 aagtttgtta aagcgatatt taccacaggc gagggtcagg atcaggcagt catccggcac
    32821 gctggtggcg aaatcggtga agtagtggcg ctcgccgcgt gcgccgtcac agccaccaag
    32881 caggaagata tgacgcagtt tttcacggct aaccagatca atcagcgtat cggctgcacc
    32941 aagcagcgtc tggcgaccaa agcccacggt gatcaggtga ggaatttcgc tgtacgggaa
    33001 gcccgccatt tgttgcgcct gggcgatgac cgcagagaaa tcttcgccat ccagatgacg
    33061 cacgccaggc cagccaacga tgctgcgggt ccagatacga tcgtcataag cgcctacggt
    33121 tgggtcgatg atgcagttcg aggtcatcac gatggggcca gggaaacgag cgaactccac
    33181 ttgctgattc tgccagccgc tgccgtagtt accgaccaga tgcttgaatt tacgcagctc
    33241 cgggtagcca tgcgcaggca gcatttcgcc gtgggtgtag acattaacgc ccgtgccttc
    33301 ggtctgttcc agcaggttgt agagatcttt gagatcgtga ccggaaatca gaatgcattt
    33361 acccgccgtc gctttgacgt tgacctgggt tggcgtcggg tgaccgtatt taccggtttc
    33421 gcctgcatcc agaatgctca tcactttgaa gttcatctgg ccgatttcca ttgaacactc
    33481 aagaagcgcg ttcatatcgg caggccaggt ccccagccac gccatgattt tatggtactg
    33541 ggcataaata tcgttgtcgt attgaccgag aacatgcgcg tgttccatat aggccgccgc
    33601 acctttcagg ccatacaggc acagcagacg caggccgaga atgttttcgc caatcgccgc
    33661 tttatctttg ttaggggtaa attctgctgc ctgacgttgc agctcgccga gatcatcgct
    33721 caccagttgc aggtcagcca tcgggttatc gacgcgcgcg ttggcatcta cagccaggca
    33781 ttgtgctttc agcgcctcgc gcaaggcaat cgcttcacga gcgtagccga caatacgcgg
    33841 agaatcgaag ttaacgttgg tcagggttga gaaaaaggca cgtggcgcga agctgtcaac
    33901 atcgtggttg atgatgccgt attcacgcgc ttttaccgcc caggcagaaa gcccttgcag
    33961 cgccgcgatg agtaaatcct gaaggtcaga agtttccgcc gttttaccac acatcccctg
    34021 cgcgtatgag cagccgtttt ctgccggagt acggatagtt tgttcacatt gcacacaaaa
    34081 catgatcaca ccttttaaag ttatatttaa tatacatgtt taagggtaag acgcttaacg
    34141 cggggataaa agggattttt catgcaactt taagggagat tgatttagcg caattttggc
    34201 ggcagggaac taccgccaga gaggtattac gcagagaaaa aggcgatgag gatcggcact
    34261 aacaggctaa gaataaaacc gtgaacaatt gccgccggga ccatatccag cccgccagta
    34321 cgttgaagaa cgggcagggt gaaatccatt gatgtggcac cgcataagcc cagtgcagta
    34381 gagcggctgc ggcgaatcag cccagggatc aacataatag caatcagttc acgggccaga
    34441 tcattaaaaa acgccgcgct cccgattacc ggaccaaaag attcggtcaa taaaataccg
    34501 gaaagagaat accagccgaa accggaggcc attgccagcg cggtattgat ggggagatca
    34561 agaataaagg cgttaattaa accaccaatt aatgaactga caaccaccac cacggcgaca
    34621 atcattcccc ggcgattaag gacaatctgc tttaaggtca tgccattatt gcgcaactga
    34681 ataccaacga ggaaaagtag caaaattaac gtgtattcac tggcttcggt cgcgtgttgt
    34741 aagaaagcca gtccactcag accaatggca aaaccaatca ctactacgcc gcacagtttt
    34801 agcgactcca gcgccatcgc aatacgcgac gggagttttt cttgctgatg gtggttgcgc
    34861 cacggcaggc ctcgctccag ccacatcagg gcggcaatat tacacagtaa aataacggta
    34921 atactgacgg cagaataatg cagaatcgcc aacaggttac tggcgaggtt atcgagaaac
    34981 gccagactga tacccataaa aaagagaata aggtaaacca tccagcttaa tagctgatta
    35041 atagctttta acgcagcttg ttggcgaagc ggaatgaggt aacccacaat caggggaacc
    35101 agaatgatta acagcccaga aaacatgaaa atccagtcct tgcaaagaag aagtcgaaat
    35161 gcgcgatgac acactactga aagcggaagg gcgagtaaag ttgcaattaa aaggaaatgt
    35221 tatgc
//
LOCUS       DAJTYM010000032        34802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTYM010000032 DAJTYM010000000
VERSION     DAJTYM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..2099
                     /locus_tag="OO575_001622"
     CDS             <1..2099
                     /locus_tag="OO575_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6664629.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2096..3184
                     /locus_tag="OO575_001623"
     CDS             2096..3184
                     /locus_tag="OO575_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6664630.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3272..4570)
                     /gene="exuT"
                     /locus_tag="OO575_001624"
     CDS             complement(3272..4570)
                     /gene="exuT"
                     /locus_tag="OO575_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6664631.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5055..6467
                     /gene="uxaC"
                     /locus_tag="OO575_001625"
     CDS             5055..6467
                     /gene="uxaC"
                     /locus_tag="OO575_001625"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6664632.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6482..7969
                     /gene="uxaA"
                     /locus_tag="OO575_001626"
     CDS             6482..7969
                     /gene="uxaA"
                     /locus_tag="OO575_001626"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6664633.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8052..8603
                     /locus_tag="OO575_001627"
     CDS             8052..8603
                     /locus_tag="OO575_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6664634.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8608..9852)
                     /gene="sstT"
                     /locus_tag="OO575_001628"
     CDS             complement(8608..9852)
                     /gene="sstT"
                     /locus_tag="OO575_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6664635.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10448..11413)
                     /gene="alx"
                     /locus_tag="OO575_001629"
     CDS             complement(10448..11413)
                     /gene="alx"
                     /locus_tag="OO575_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6664636.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11684..12682)
                     /locus_tag="OO575_001630"
     CDS             complement(11684..12682)
                     /locus_tag="OO575_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6664637.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12761..13453)
                     /locus_tag="OO575_001631"
     CDS             complement(12761..13453)
                     /locus_tag="OO575_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6664638.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13530..14033)
                     /locus_tag="OO575_001632"
     CDS             complement(13530..14033)
                     /locus_tag="OO575_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6664639.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14118..15254
                     /gene="rlmG"
                     /locus_tag="OO575_001633"
     CDS             14118..15254
                     /gene="rlmG"
                     /locus_tag="OO575_001633"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6664640.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15539..15853
                     /gene="higB"
                     /locus_tag="OO575_001634"
     CDS             15539..15853
                     /gene="higB"
                     /locus_tag="OO575_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6664641.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15850..16266
                     /gene="higA"
                     /locus_tag="OO575_001635"
     CDS             15850..16266
                     /gene="higA"
                     /locus_tag="OO575_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6664642.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16311..18329)
                     /gene="fadH"
                     /locus_tag="OO575_001636"
     CDS             complement(16311..18329)
                     /gene="fadH"
                     /locus_tag="OO575_001636"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6664643.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18555..20906)
                     /gene="ygjK"
                     /locus_tag="OO575_001637"
     CDS             complement(18555..20906)
                     /gene="ygjK"
                     /locus_tag="OO575_001637"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6664644.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20923..21342)
                     /locus_tag="OO575_001638"
     CDS             complement(20923..21342)
                     /locus_tag="OO575_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664645.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21320..21673)
                     /locus_tag="OO575_001639"
     CDS             complement(21320..21673)
                     /locus_tag="OO575_001639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664646.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21674..21994)
                     /locus_tag="OO575_001640"
     CDS             complement(21674..21994)
                     /locus_tag="OO575_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664647.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22128..23561)
                     /locus_tag="OO575_001641"
     CDS             complement(22128..23561)
                     /locus_tag="OO575_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6664648.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23624..24073)
                     /locus_tag="OO575_001642"
     CDS             complement(23624..24073)
                     /locus_tag="OO575_001642"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6664649.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24070..27156)
                     /gene="ebgA"
                     /locus_tag="OO575_001643"
     CDS             complement(24070..27156)
                     /gene="ebgA"
                     /locus_tag="OO575_001643"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6664650.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27339..28322)
                     /gene="ebgR"
                     /locus_tag="OO575_001644"
     CDS             complement(27339..28322)
                     /gene="ebgR"
                     /locus_tag="OO575_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6664651.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28540..28871
                     /locus_tag="OO575_001645"
                     /pseudo
     CDS             28540..28871
                     /locus_tag="OO575_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28913..30292)
                     /gene="ygjG"
                     /locus_tag="OO575_001646"
     CDS             complement(28913..30292)
                     /gene="ygjG"
                     /locus_tag="OO575_001646"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6664652.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30710..32230
                     /gene="aer"
                     /locus_tag="OO575_001647"
                     /pseudo
     CDS             30710..32230
                     /gene="aer"
                     /locus_tag="OO575_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32384..33007)
                     /gene="nfeR"
                     /locus_tag="OO575_001648"
                     /gene_synonym="yqjI"
     CDS             complement(32384..33007)
                     /gene="nfeR"
                     /locus_tag="OO575_001648"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6664653.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33284..34029
                     /gene="nfeF"
                     /locus_tag="OO575_001649"
                     /pseudo
     CDS             33284..34029
                     /gene="nfeF"
                     /locus_tag="OO575_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34083..34158)
                     /locus_tag="OO575_001650"
     tRNA            complement(34083..34158)
                     /locus_tag="OO575_001650"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34123..34125),aa:Ile,seq:cat)
     gene            34283..34789
                     /gene="mug"
                     /locus_tag="OO575_001651"
                     /pseudo
     CDS             34283..34789
                     /gene="mug"
                     /locus_tag="OO575_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8489 a         8949 c         9145 g         8219 t
ORIGIN      
        1 atttcaacgg ttatgatgca gatgatgtca gtgacagtaa tactgacgtc agcgatctct
       61 attatcgtta tgatttttta cgtcgttatt atgtgcaggc gggtcgcatg gacaaccgca
      121 cactatttaa tgcacaaggc gggaacttta cctttaactt tctgccactc ggtgcaatcg
      181 atgggatgcg tatcgggtcg acactgagct atttaaacca ggcgcaaagc cagcaaggaa
      241 ccccagtaat ggttctgctt tcgcgcaatt ctcgtgttga cgcttatcgt aatgagcaac
      301 ttttgggatc gttttatctc aatagtggtt cgcaatttat tgataccagt tcctttccgc
      361 caggcagcta cagcgtggca ttaaaagtct acgaaaataa ccaactcacc cgcaccgagc
      421 ttgtgccgtt taccaaaacc ggcggtctga ctgacggaaa tgcgcaatgg ttcttacagg
      481 caggtaaaac tacatcacag gtttctgatg atgaaagttc agcttatcag ctaggagtac
      541 gcctgccatt acatccgcaa tatgagctct acgcagggct ggcgaatgct gatgatgtga
      601 gtgctttcga gttaggtaat aactggacgg cagatttagg cagggtgggg aatcttgcaa
      661 tcagcgccag cgtgttccgt aacgatgacg gcggcaaagg tgatatgcaa caggccaact
      721 ggagtaatcc gggatggccg acgttgggct tttatcggac caactctgac ggcgatgctt
      781 gtacaaccga cagcagagag agctataacg ccttaagctg ttatgaaagt atttccgcga
      841 cagtttcact gaattttgtc ggctggaata tgatgctggg ttatacctgc acacaaaata
      901 acactgatga tagtttgcgt tgggataaac agcagagctt tgaaaataac tatcttcgcc
      961 agacaacagc gcaaagtatc tctgaaactg tacaacttag cgcttcccgc gctattgtga
     1021 tgcgtgactg gattttgagt acgtcagtcg gtgttttcca tcgtaatgac aacggtggcg
     1081 ataacgacga caacggcttg tacttatcgt tttcgttatc tgacacgcca acgatggata
     1141 gcaataacaa cagccattca accaatgtgc ctacggatta tcgttatagc gaacaggatg
     1201 gcgatcaaac gtcatggcag ttatcgcata ccttttataa cgattcattc agccataaag
     1261 aacttggcgt aaccgtcgga ggcctgaaca ccgataccat aaacagcgcg gttaacgggc
     1321 gttgggatgg gcaatacgga aatgtctacg ctaccgtatc tgatagttat gaccgtaaga
     1381 atcatgatca tctttcggcc tttacgggca cttatagctc tacgctggct gtcagtcgct
     1441 atggcgttaa tttgggagcc agtggtacag acgatttgct gggtgcggta ctggtggatg
     1501 tgaaaggctt ctctgaacag gatgaagaga gtcaggatct gcaactcgaa gcgcgggtgg
     1561 caggcagccg aactttgcag cttggtcaaa gtgacagtgt gttgttccct tatcctggat
     1621 ttcagtctgg ttttgttgag gttaacgaca gtagccaggg caatcagcag gggacaacaa
     1681 acatcattaa cggtgcaggg aatcgtgaat taatgttgtt gcctggcaag ctgcgctatc
     1741 gcgaagtgtc tgccagcttt aactacaact atatcggtcg tttattactg ccggcagcgg
     1801 tgaaaaaatt cccgattgtg gggctgaaca gcgccatgtt actggtagct gaagatggcg
     1861 gatttacact tgaaattaac ggcagcgaaa aagagctcta tctgctttcc gggcagcaat
     1921 tccttaagtg tccactgagt gttgtaaaga aacgcgccag cattcgttac agcggagatg
     1981 ttacttgtag tgtggtgact tattcacaat taccggagtc gattcaggtt caggcacagt
     2041 tgaaacagcc taaattacgt ggaaacgttc agacggcgca aagggaggtt gcaccatgag
     2101 aaatcgattg attgcggcga tattgggttt gtttggcacg ctcactggcg ttcaggcagc
     2161 tcctgacgtg accagtgaaa ttacgtatga tttggcatct ggtagagcgg attattactt
     2221 ctggaaggat gaagcgtcgg caggaaataa tggatatatg tggtatgaat gttcgtatcc
     2281 tgacctccaa caaacctgta cagctaatgg aaatatatcg acagtacaaa tctatttaac
     2341 tgaacaacgc agtgggatgc gttggccggt aaaactcaaa ggatttaaaa cagccattgt
     2401 aagtagcgat gaagcgccgc taggatgcaa ggggggcaaa gggcttcaga cgaatcttaa
     2461 ggattctaat agatcttcat gtacagaaga tggtcaacat tattatatat acgatacaaa
     2521 gtttcttacg ctctaccttg agcagacaga gatgaagaat ttgccgattg gtggcgtctg
     2581 gaaggggaaa gttaaattac attcgaacag cccggcccag gactatttcg caaatattac
     2641 cctgaatacg ctcgacccca accatattga cgtgttcttc ccggagttcg cccacgccac
     2701 gccaagggtg cagttagact tgcatccaac aggaagcgtt aacggcagca actacgcgca
     2761 agatctgacc atgttggaca tgtgcctgta cgatggtttt aacggtaatg ccatcagtta
     2821 tgaaatcatg ctcaaagatg aagggcgacc agctgcaggg cgcagagacg gttacttctc
     2881 tatctatcgt cagggaggga ccaccaccga cgagggagaa cgcattgatt accgggtcaa
     2941 aatgtacaac ccggaaaccg gtgggcaaat tgatgtgcgc aataatgaaa atatggtctg
     3001 gaacagcatt aacctgaaac gtgtgcgtcc ggtggtactg cccggtattc gctatgccgt
     3061 gatgtgtgtg ccgacaccct tgactctggc agtcgataaa ttcagcgtga tggataaaca
     3121 ggccgggtac tacatgggca aattgtcagt aatctttacg ccttccttgc caaccatcaa
     3181 ttaaccctgc aaaccggaca aacgcttttt tctatataaa cgacgaagcc gcctgataag
     3241 gcggcttcgt cgttataact tacgttaaac gttaatgttg cggtgcggga tcgttatgtg
     3301 tttcctgcgc cacctcaatc gccggtttgt tctgcaatac ggtccagata accagcgcac
     3361 ccagcaggtc aaatactgcc agaactgcga acagcgggct gaagccgatg gtgtcagcca
     3421 gtgcaccgac aaccagcgca aacagcgtac ttgccagcca tgcggacatc ccggttaaac
     3481 cgttcgccgt cgccacttcg ttacgaccga acacgtcaga agagagcgta atcagcgcgc
     3541 cagacagtgc ctggtgggca aaaccaccga tacacagcag cataattgcg acatacgggt
     3601 tggtgaacag accgatcata cccgggccaa tcatcagcac agcacccagc gttacgacca
     3661 tcttacggga aacgatcagg ttcacaccaa accaacgctg gaacagcggc ggcaggtaac
     3721 caccgaggat acaaccgagg tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct
     3781 ctttcaggtt aaagccgtaa actttaaaca tgaacagcgg gatccacgcg ttaaaagtac
     3841 cccaggccgg ttctgcaagg aaacgcggca gtgcgatacc ccagaactga cggttacgca
     3901 ggatctgacc aacggacatt ttcttcgccg tgctcacctg gtgctgggct tcctgaccat
     3961 taataatata gtcgcgttct tcatcggtca gatgcttctg gtcgcgcgga tgtttataga
     4021 aaatcagcca tgccatcgcc cagataaagc tcaatgcacc ggagatgata aatgccatct
     4081 gccagctgtg catcacgatt gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg
     4141 aagaacctac gttaaagtag cctactgcga tggaacgctc tttcgccggg aaccattcgg
     4201 agctggcttt cagacccgcc gggatcatcg ctgcttccgc ggcaccgacc gcaccacgag
     4261 caacagctag gccaccccag ctacctgcca gcgcggttgc accacagaac acggcccaca
     4321 gcacagcaaa cattgcataa ccgattttcg tacccagcac atccagtaca taacctgcta
     4381 ccggttgcat gaccgtataa gcagcagaat aggctgcgat gatataggaa tactgttggg
     4441 tggagatgtt taactcttcc atcagagttg gcgcagctgc cgccacagtg ttacgcgtca
     4501 ggtaaccaag cacggtgccg agcgtcacca gtgcgatcat ataccaacgt aaccctttaa
     4561 ttttacgcat gtaaaacctc atcgttttgt tatatccgca ggaagcgcgg ccatcatccc
     4621 gccagaggct gaagatgtta gttacgaccg ggtaaccgaa aaaaaaaacc ggcacgcccc
     4681 gaacgttgcc atgaaagttt cgtgctaatt cggcttccgt accggttaac ccgattcatc
     4741 taacaaatga atgaatcggc aatttgcatt ccgtcgactt atgatttgcg acggcagaaa
     4801 gataacttgt catacaacct taaaaggtga gagccatcac aaatgtgcga atatttgtag
     4861 ggacattacc tgacgacagc gaggccagta ctggcgcggc ctgcagcgag atttaccact
     4921 ttgagagtaa tttttttaac tacgtttatt gatctaactc acgaaaatat cttcggactc
     4981 tggaaattgg tgtgataact ttgtcagcat cgcaccataa gcaagctagc tcactcgttg
     5041 agaggaagac gaaaatgact ccgtttatga ctgaagattt cctgttagat accgaatttg
     5101 cccgccgtct gtatcacgac tacgcaaaag accagccgat tttcgattac cattgccatt
     5161 tgccgccgca gcagattgcg gaagactatc gttttaaaaa cctgtatgac atctggttga
     5221 agggcgatca ctacaaatgg cgcgctatgc gtaccaacgg tgtggctgag cgtctgtgta
     5281 ctggtgatgc gtctgaccgt gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta
     5341 tcggcaaccc gttataccac tggacgcacc tcgaactgcg tcgtccgttc ggtatcactg
     5401 gcaaattgct ttctccgtca actgccgatg aaatctggaa cgaatgtaac gaactgctgg
     5461 cgcaggataa cttctccgcg cgcggcatca tgcagcagat gaacgtgaaa atggtcggca
     5521 ccaccgatga cccgatcgat tctctggagc atcacgcaga gatcgccaaa gacggctctt
     5581 tcaccatcaa agtgctgccg agctggcgtc cggacaaagc cttcaatatc gaacaggcga
     5641 cctttaacga ctacatggcg aagctgggcg aagtttccga taccgacatt cgccgctttg
     5701 ctgacctgca aactgccttg accaaacgtc tggatcactt cgccgctcac ggctgtaaag
     5761 tgtctgacca tgcgctggac gtggtgatgt ttgctgaagc gaacgaagcg gaactggaca
     5821 gcattctggc gcgccgtctg gctggcgaaa ccctgagcga gcacgaagtg gcacagttca
     5881 aaactgcggt actggtgttc cttggtgccg aatatgcacg tcgcggctgg gtacagcagt
     5941 accacattgg cgcgctgcgt aataacaacc tgcgtcagtt caaactgctg gggccggatg
     6001 taggctttga ctccatcaac gaccgtccga tggcggaaga gttgtctaag ctgctgagca
     6061 agcagaacga agaaaacctg ctgccgaaaa ccattctcta ctgcctgaac ccgcgcgata
     6121 acgaagtgct gggcaccatg atcggtaact tccagggcga aggtatgccg ggcaaaatgc
     6181 agttcggttc cggctggtgg tttaacgatc agaaagacgg tatggaacgt cagatgaccc
     6241 aactggcgca gctcggtctg ctgagccgct ttgtcggtat gctgactgac agccgtagct
     6301 tcctgtcata cacccgtcac gaatacttcc gccgcattct gtgccagatg atcggtcgct
     6361 gggtggaagc gggcgaagca ccggcggaca tcaacctgct gggcgagatg gtgaaaaata
     6421 tttgctttaa caatgcgcgt gactacttcg ccattgaact gaactaaggt ctggggttga
     6481 tatgcaatac atcaagatcc atgcgctgga taacgtcgcg gtcgctttag cagatttggc
     6541 tgaaggcaca gaagtcagtg tcgataacca gactgttacg ctgcgccagg atgttgctcg
     6601 tggacataaa tttgcgttaa cggatatcgc aaaaggggcc aacgtcatca aatatggcct
     6661 gccgattggt tatgcattgg cggatattgc ggcgggggaa cacgtccacg cccacaatac
     6721 gcgcacgaat ctgagcgatc tggatcagta tcgctatcaa cttgattttc aggatctgcc
     6781 tgcgcaagcg gcagatcctg aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt
     6841 gcgtaacgag ctgtggatcc tgccaaccgt gggctgtgtc aacggcatcg cgcggcaaat
     6901 ccagaaccgt ttcctgaaag agaccaacaa cgccgaaggt accgacggcg tgttcctctt
     6961 cagccacacc tatggctgct cacaactggg cgacgatcac atcaacaccc gcaccatgct
     7021 gcaaaacatg gtgcgccacc cgaacgcggg cgcagtgctg gtgattggtc tgggctgtga
     7081 aaacaaccag gttgccgcat tccgtgaaac gttgggcgat atcgatcctg aacgcgttca
     7141 tttcatgatc tgccaacagc aggatgatga gatcgaagcc gggatcgagc atttgcatca
     7201 gctgtataac gtgatgcgca acgataaacg cgagccaggc aaactcagcg aactgaagtt
     7261 tggtctggag tgcggtggtt ctgacggtct ttctggtatt actgctaacc cgatgctggg
     7321 gcgtttctct gactacgtga ttgctaacgg cggtactacc gtactgaccg aagtgccgga
     7381 gatgtttggc gcagagcagt tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa
     7441 actggtcacc atggtcaacg acttcaaaca gtactttatt gcccatgatc agccgatcta
     7501 cgaaaaccca tcaccgggga acaaagcggg cggtatcacc acgctggaag acaaatcact
     7561 tggctgtacc cagaaagcgg gttccagtgt cgtggttgac gtgctgcgtt acggcgagcg
     7621 tctgaaaacg ccagggctga acttgttaag tgcgccgggt aacgatgccg tagcgaccag
     7681 cgccctggcg ggtgcgggct gccatatggt gctgttcagt actggtcgcg gcacgccata
     7741 cggtggattt gtgccgacgg tgaaaatcgc caccaacagt gaactggcgg cgaagaaaaa
     7801 acactggatc gactttgacg cgggtcagct gatccacggt aaagcgatgc cgcagttgct
     7861 ggaagaattt atcgacacca tcgttgagtt tgccaacggt aagcaaacct gcaacgagcg
     7921 taacgacttc cgcgaactgg cgatcttcaa aagcggcgta acgctataaa gtcgaaatga
     7981 gttgttaata aaaaacggcg tttcataatg tgaagcgccg tttgcttttc catacctgct
     8041 aaggatttat catgaccgcg tattggctgg cccagggcgt gggtgtcatc gcctttctga
     8101 ttggtatcac aacatttttc aatcgtgacg aacgtcgctt caaaaagcag ctttcggtct
     8161 atagcgccgt tattggcgta catttttttc ttctgggcac ctatcccgct ggtgccagtg
     8221 ccatccttaa tgccattcgt acattgatta ccttacgcac gcgcagctta tgggtaatgg
     8281 cgatttttat tgtgctgact ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac
     8341 tattgccggt tatcggtacg attgtcagta cctgggcgct gttccgctgt aaagggctca
     8401 ccatgcgctg cgtgatgtgg ttttcaacgt gttgctgggt gattcacaac ttctgggcgg
     8461 ggtcgatagg cggcacgatg attgagggta gttttctgct tatgaatggc ctgaatatca
     8521 ttcgtttctg gcggatgcag aaaaggggaa ttgatccgtt taaagtagag aaaacccctc
     8581 ccgccataga cgaaaggggt taaacaatta attacgcagg gcgctatttg ccagacgatc
     8641 gtcttccgcc tggcaagctg ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt
     8701 ttcgcaagag tcctgcaata cgccgataat aaagccgacg gcaaccacct gcatggcgat
     8761 atcgttcgaa ataccgaaca tattacaggc cagtgggatc agcagcaaag aaccccccgc
     8821 cacgccggat gcgccacagg cacacagaga agccactacg ctcaacagca gcgctgtggg
     8881 cagatcgacc ggaataccca gcgtattaac cgcagccagc gtcaacacgg taatggtgat
     8941 tgctgcgccc gccatattga tggtggctcc cagcggaata gaaactgaat aggtatcgcg
     9001 atccagattc agcttttcac acagcgccat attcaccgga atgttcgctg cagagctgcg
     9061 ggtgaagaag gcatacacac cgctttcgcg caggcacagc agcaccagcg ggaacgggtt
     9121 acgacgaatt ttccaccaca ccagcaatgg gttaaccacc agcgccacca gtaacataca
     9181 gccaaccagc acgaccagca gttgcgcgta gccccacagt gtggagaaac cggtggttgc
     9241 cagggtagaa gaaaccagcc caaaaatacc gaacggtgcg aagcgaatga ccagtttcac
     9301 cataaaggta acggcattcg acatatcatt gaccaggttt ttggtggtct cgttaccgtg
     9361 acgcagtgcg aagccgaggc cgatcgccca caccagaatc ccgatgtagt tacctttcag
     9421 cagggcgtcg atggggttgg aaaccatgct cattaccagt ccgcgcatca cttcgacaat
     9481 gcctgacggc ggcgaaatat cacccgcgct actggacaag tgcagggtag aagggaaggc
     9541 aaagctgaag actactgcgg ccagagctgc agagaaggtg cccagcagat agaggaacaa
     9601 aataggacgg atattggttt tctgcccgtg ctggtggtta gcaatagatg ccattaccag
     9661 catcaacacc aggatggggg caacagcttt cagtgcgccg acgaacaaag tacctaacag
     9721 accaacagct tccgccgcgg gttttgagat ccatgccaga agaatcccca gaacaaggcc
     9781 gaccaggatt tgttttacca ggctgccatg agccagacgc cggaatagcc ccggtgaacg
     9841 ttgcgtagtc atttttcgat cctttcattg tgttgtcgca catccctggt gcgttctgtc
     9901 gacgcatctt tcaggatgta acaaagtgtt tgcaaggtcg agtataagga aagttacagc
     9961 ttcgggaagc gaaaaatgct ggattttaaa gagaggtcat gttttgcaac ttaatggttg
    10021 catttattta acataaacag agggaaggac tggctactaa ggctgattag ataacatatg
    10081 ggcaaaacta tttggaatat gagaacggtt gcgctccttt aacgtgttat gccatataaa
    10141 gtcactggcg cagggagcgc gcagggggcg gccaatcgcc gccgccccct gcacccccag
    10201 gctctggcga acaaaatcgc cgctgcgcgg ttccctcagc ctatcccttg cggctaccgg
    10261 gtcgggcgca aggtaacgtc cctgtaagat gcgccctcgc cccggcctcc agggaacgct
    10321 tcggcgattt tgacgccacc aacacccgtg cggtcttttt gtaaagagct ataagccgat
    10381 tacataatta atagctcacc tttgtctgga atgagttatc tctaggaatg gcttaatata
    10441 cagcgaatca ttctccccgc tgcttatcat gccgataatt cacccaggcg ttgataataa
    10501 acgtcatcac cagaatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga
    10561 agtcgacaat cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga
    10621 gcatcgagaa acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga
    10681 ttgcaaacag gtttgaggtc agcacaatga acgggtcagt ggtcacggcg aagatagccg
    10741 gaatgctatc cacggcaaaa atcacgtcgc tcaattccac cagaatcagt accagcatca
    10801 gcggtgtggc gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt
    10861 cggtcatgcg caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt
    10921 catcttcatg ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat
    10981 acaggatcca gtcgaactgc gaaatcagcc agctgcctgt gaagatcatg atggtacgca
    11041 gaacaatcgc cccgagcacg ccatacacca gcacgcgacg ttgtaatgcc gccggaacgg
    11101 agaaatagct gaacaacatc agccagacaa agacgttatc gaccgccagc gatttctcaa
    11161 tcagataacc cgtgagaaag gccagggcct gtggatcggc aacggcgcga ccttcggttt
    11221 gcaccagata ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc cagaccagcg
    11281 accaggccgc agcctgtttc atggtcatgg catgtgcccc acgacgcccc tgcaacaaca
    11341 ggtcgatagc cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc
    11401 cgacagtatt catagaagtt ccttacacat aaaaaaacgg ccaacgtcgg aagacgtcag
    11461 ccgctgcttt ttatgcatag acctcgcctt ccggcaaggt ctcacttaca acaaaaaagg
    11521 aatacgtctg cgtattcctt tcgggttgcc cggtgaccgg atgtggtttt tcactcatcg
    11581 taatgacgat cgaccggcaa tgaagttact cccctttgca ggtaacaaag tatgacagac
    11641 cattcagtca gtcaatggtc tgttacatac cttttacact gttttacttt ggcaagtata
    11701 gttttacgct atctgccgga aaaatcaccc cagtttggcg gcggatctcg gtcagcagtt
    11761 tggctgtgat gcgactgacc gccagccctg gatgatccac cagatgctca tccaccaggg
    11821 tagcgaacag ctctgcttcg tagagcatgg tattaatatg ctgcggctgg gtgagatcct
    11881 gcatctggct gccacgcggc acaaagcaga ctttctggca ttcagacagt ttttcaatca
    11941 ccagcgatcc cgcttcgccc tgaatctcgc ttcccaggac agaatcactg acctttgagt
    12001 gctgcaaggt gacgctgaaa tcaccgtaat ccatcaccac cacgccgtgg gcgtcaacgc
    12061 cgcttgccag caaactcgcc gttgcctgca cgcttttcgg ctcaccaaat aacgccaccg
    12121 ccgacgccag gcagtaaaag ccgatatcca taattgaacc gttagagaat gccggattaa
    12181 aggtgttggg attctcaccg tccaggtaac gttgataccg cgaggaatac tggcaatagt
    12241 tgaaaaagac tttacgcagt ttgcctgctt tcggcagcgc ctggcgcaac aaatgaaagt
    12301 tcggcaggca ggcggtttta aatgcctcaa acagcaccac ctgattttcg cgcgcacagg
    12361 caatggcggc atccacttcc gccagattcg acgccagcgg tttttcgcaa atcacatgaa
    12421 ttttatggct aaggaaaagt tgtgtctggg aaaagtgcag ggaattcggg ctggcaatat
    12481 acaccgcgtc aatggcatcg ctttccgcca tcgcttccag cgacgtaaac agatgctcga
    12541 cagaaaaatc attggcgaag tgctgagcct gctcaaggct gcgggaatat acggcggtta
    12601 acttgtattt accgctctca tgggcggcct cgacgaactg gcgagtgatc cagttcgtac
    12661 caatcacagc gaaacgtatc ataacgcgtg gatactccat aaagggattc tgtcgtcaat
    12721 gtagcacgcc tttttcatct tcgacgtgcg cagatacgca ttattctttc gccggacagc
    12781 catgctcact aaacggccca atcatgaccg atggcccgag atatttcggc tggcgatgga
    12841 ggatttttgc gtcaatcacc gcgctgacac gagcgagttt ctcccgcagt tgagtataaa
    12901 aaccgtgctt catattgagg tctggcgcgt taaacccgat agcgtcgatg ccgtactgtt
    12961 ttgccagcca gatggtgcgt tggttatgaa attcttgcga gataatggtg atatggtttt
    13021 ccccaaacac ctttttggca cgcactaccg aatccagcgt tgagaatccg gcatagtcgc
    13081 aaaagatcac tttcgccggt acgcctttag cgatcaaagc ctgctgcata cctgatgctt
    13141 catcgtaatt tttacgtccg ttatcgccac tcaccagtag ccatttcact tttccggcgt
    13201 ggtacaacgc tgctgcggta tcaatacgtc gggtaaagta gcgattaccc ggcctcgccc
    13261 ccagcaataa acccacgtta cgcgccggga cggcgttgac atcgccccag gtcagctgct
    13321 tgctggcgtt gaccataacg cgatcggcga taaaaatggt cgctcctgcc acaagtaaca
    13381 gtaagcaggc aatctttaga gtccggcgag aaaaacaaat acggagaaga aggcgggcaa
    13441 atgcgcgcag catggtcgtt atccttaacg aaagggggga ggtcgatttt aggcgctaaa
    13501 gaaagaaaag ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc
    13561 gttagccaca gccgggtgtc gaactcaagc tggtggtact gcggctccat atggcagcac
    13621 aattgataga acgctttgtt atgctctttc tccttcaggt gcgccagctc gtgcaccacg
    13681 atcatgcgca aaaacggttc cggcgcgttg cgaaacacgg tagcgacgcg gatctctgct
    13741 tttgctttca gcttgctgcc ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt
    13801 tttagcacat ggatcttatt gtcatacatc actttattga tcggcggggc attacgcaga
    13861 aactgatttt tcagatcctg agtatattgc cagagagctt tatcggtggc gtagtcgtgt
    13921 gttcccggat agcgttttgc cagcacatcg cccagacgct gttcgttaat cagtgttcgc
    13981 acctgcgaaa gcagctgctc gggataaccc tgaagataag taagattgct cataaccgcc
    14041 cacgaaaaag agaaaaaggg tatactcacg cacccttttc aggggcaacg tcgaaatttt
    14101 atcattcagg agggccgatg agccacttag acaacggttt ccgttcactg acactacaac
    14161 gttttccggc gacggatgac gttaacccgc tacaggcgtg ggaagcggca gatgaatatt
    14221 tgctgcaaca gttggacgac acagaaatcc tcggcccggt gttgatcctg aatgatgcct
    14281 ttggtgcgtt aagctgcgcg ctggcggaac ataagccgta cagcattggc gactcataca
    14341 tcagtgaact ggcgacgcgc gagaatttac gcctaaacgg gattgatgag tcgagcgtga
    14401 agtttctcga cagcaccgcc gactacccgc aacagccggg tgtggtgctg atcaaagtgc
    14461 cgaaaacact ggcattgctg gaacagcaac tgcgtgcgct gcgcaaagtg gtcacgtcgg
    14521 atacacgtat tattgccggt gccaaagccc gtgacattca cacttccacg ctggaactgt
    14581 tcgaaaaagt gctcggcccg accaccacca cgctggcatg gaaaaaagcg cgcctgatta
    14641 attgcacgtt caatgaaccg ccgctggccg atgcgccgca gaccgttagc tggaagctgg
    14701 aaggtactga ctggactatc cataaccatg cgaatgtctt ctcccgcacc gggcttgata
    14761 tcggcgcgcg cttctttatg caacatctgc cagagaatct cgaaggggag attgtcgatc
    14821 tcggttgcgg taatggcgtt attggtctga cgctgcttga taaaaacccg caggcgaaag
    14881 tggtgtttgt cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca
    14941 acatgccaga ggcgttggat cgcagcgagt ttatgatcaa caacgcgctc tccggcgtgg
    15001 agcctttccg ctttaatgct gtgctctgca accctccgtt tcaccaacaa catgcgctga
    15061 ccgataacgt cgcctgggag atgttccact acgcccgccg ctgcctgaaa atcaacggcg
    15121 agctgtatat cgttgccaac cgccatctgg attacttcca taaactgaag aaaattttcg
    15181 gcaactgcac cactattgcg acgaataata aatttgtggt gctgaaagcg gtgaagctgg
    15241 gacgtcgtcg gtaagattgg gagttacctg ccggatgcgg cgtaaacgcc ttatccggcc
    15301 tacgttcagc acgttacccc caggccggat aagacgcggc aagcgtcgca tccggcaatg
    15361 tctgctcagt tctggaatcg cgcttccata aaacaaaaaa tccctgtcac catcctctac
    15421 attctcttgt ttagcgtttt tctacgttta ttcttccgtc acacagataa attcatccgt
    15481 tgccaatctg tcaacggatg ttatcatatt gccaatttgt caacggacgt gatgaagaat
    15541 gcacctgata actcaaaaag cattgaaaga tgctgcggaa aaatacccgc aacataaaac
    15601 ggagttggtg gctctgggga acacgattgc taagggatat ttcaaaaaac ctgagtcatt
    15661 aaaagcagta ttcccatctc tggataactt caaatatctg gataagcatt atgttttcaa
    15721 tgttgggggc aatgaattac gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta
    15781 catacgtgaa gttatgacgc ataaagaata cgatttcttt accgctgttc atcgtactaa
    15841 ggggaaaaaa tgattgcgat tgccgacatc ttgcaagcag gagaaaagct aactgctgtg
    15901 gcaccttttc tggcgggtat tcagaacgag gaacaataca cccaggcgct ggaactggta
    15961 gatcatctgc tgctcaacga tcctgaaaac cccttgctgg atctggtgtg tgccaaaata
    16021 accgcgtggg aagaatcagc gcccgaattt gcggaattta atgccatggc tcaagccatg
    16081 cctggcggta tagccgtgat tcgtaccctt atggatcaat atggtttaac cctttccgat
    16141 ctgccggaaa ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa gaggaaatta
    16201 acgctggaac acgctaaaaa attggcaacg cgattcggca tttctcccgc cttgtttatt
    16261 gattaacgcg acgtgccgaa tgcgttgcat ccggcgcttc aaataacgat ttaaatctcc
    16321 agcgccagcc gtgttccctg ggcaattgcc cgtcgtgcgt ccagctccat agccacatcg
    16381 cagccgccaa ttaaatgcac agttttcccg ctatcaatca gcggttgcgc cagcgcgcgg
    16441 tttggctctt gccctgcgca gatcaccaca ttgtccactg ctaatacctg cgtttcgccg
    16501 ttgatcacca catgcagccc gtcatcgtca atcttctgat aacttacgcc tgggatcatt
    16561 ttcacacccc gcgagagcag ggtggtgcga tggatccagc cggtggtttt gcctaacccc
    16621 tgtcccggtt tgctggcttt gcgctggagc atcacaattt gccgtgggct acgcgggatc
    16681 tgcattccct gcgggcttaa gccaccagcc tgttgtaggc tgctgtcgat cccccactca
    16741 ttacagaacc tggcgatatt ctggctggtg gattcgcccg gctgacttaa atacatcgcc
    16801 gtatcaaaac caatcccgcc acaaccgatg atggcaacct tgttgcctac cggcgctttg
    16861 tcgcgcagta catcgagata actcaatacc ttcggatgat cgatcccgtc gataggcgga
    16921 acacgcggca cgatcccact ggcgaggatc gtttcatcga acgcctgtaa ctgatccgcc
    16981 gtcacggtgt gattgagttt cagcgtcacg cccgtcactt cgatcatccg gcggtagtag
    17041 cgcagcgttt cgtaaaactc ctctttgccg gggatctgtt tggcgatatt aaactgcccg
    17101 ccgatctcgc tgtgcgcatc aaacaatgtt acctgatgcc cacgcgccgc cgcgttaatg
    17161 gcaaacgcca gcccagcagg tccagcgccg accaccgcca gatttttttt ctgcacggcg
    17221 ggaaggattg gcattttggt ttcgtggcag gcgcgaggat tcaccaggca cgaggtgact
    17281 ttgccaacga agatctgatc gaggcagccc tggttgcagc caatacaagt gttgatctca
    17341 tcggctcgcc ctgattgcgc ttttgacagc agttccgcat ccgcgagaaa cggtcgcgcc
    17401 atcgatacta tatcggcatc gccgcgcgag agaatatcgt cggcaacctg cggatcgtta
    17461 atccggttgg tggttaccag cggcagcgag acgtggcctt tcagtttgcg cgtgacccag
    17521 ctaaatgcgc cgcgcggcac gggcgtggcg atggtcggaa tccgcgcttc gtgccagcct
    17581 attccggtgt tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag ttctaccgtt
    17641 tcggcaaaag tgccgccgcc ttcgactagg tcgagcatcg acaaccggta aataataatg
    17701 aagtcgttgc cgacgcgttc gcgcaccgta cgcactactt ctacggcaaa tcgcatccgg
    17761 ttgcggtaat cgccgcccca ctggtcacta cgctgattgg tgcgcagcgt cagaaattcg
    17821 ttgatcaaat acccttcaga gcccatcact tcgacgccat cgtatccggc ctcccgcgcc
    17881 agttgcgcgc agcgggcgaa atcgtcgatc agttgcagga tctcttcatg ggtgagttcg
    17941 tgaggaacga aacggttgat gggggcctgc aatgcggacg gtgcgaccag atgcggttgg
    18001 tagctgtagc gcccggtatg caaaatttgc agggcgattt tgccgccttc ctgatgtacg
    18061 gcttcggtaa tggtgcgatg gtgtgggatc tggctggcgt cgttgagcat tgcgccacct
    18121 tccatgccaa cgcctgttaa atctggtgca atgccgccgc tgacaatcag cgccacgccg
    18181 tgacgggcgc gttcggcata aaacgcagcc agccgttcag caccgtccgg gtattcctcc
    18241 agcccggtgt gcattgaacc catcaacacg cggtttttta acgtggtaaa acctaaatcc
    18301 agcggggcga acagcgacgg gtagctcata aaattgtcca gtatgtaaaa taattgttat
    18361 gtagtcggat gagttctaat ttagccgtcg atggagaaaa gggaaaaggg gagtgcgggg
    18421 tttgtgatgg gattcaaaaa gaatacgccc ggtggtaacg ccggatgcga cgcttgccgt
    18481 gttttatccg gcttggggga ttgcacatgt agggcggata aggcgttcac gccgcatccg
    18541 ccagccgttg caaattactg cttacggaaa aaatcgttat acaacatata caaatgagcg
    18601 gcactccagg agaaatttgg tgcgccttgc tgcgcgcctg tcagcgggtt gtaattctcc
    18661 tgaattgggc catcggcggt taatcctttg gcgtgcctga agaacgtatc cgccagcttc
    18721 agggcatcat cacgataacc gtaacgctcc atccctttca gaccaaacca gaactgatcc
    18781 acccatacgc gcccgcgcca gtagatatca gcgccaaaag ccggattggt taacgccgcc
    18841 gttcccagcg gaacaaatgt gttgaactct ttaggatcga gcatcacctt caccaccgcg
    18901 tcggcattgg cctgcgttgc cgcaccgtta aacagcggcg accagccttc cggcccttta
    18961 ccgcgctcaa caatcggttt gcccgcgcag ccgttcgcca gtggtttatc ttcaatacgt
    19021 acgtcatagt agaactgggt agtcgggtcg aacatacagg tgttgatgta gtccgcgagc
    19081 tgctgtgcca actggcgata gcgtttggcc tcttctggct taccgaggat ggttgccatc
    19141 tccgccagat aatggttatc gctgtacata tagctggcct gatccaccga ctcctgcaat
    19201 agcgagtagc ccagcaacgt tccgtcctga ctgcggtttt cggcgaattt caccgtccag
    19261 tcgctacgtt tgccgccatt agcgacatat ttatccaact gttctttgtc gataaaacca
    19321 aagacggcgg catcatcacg ccccgattcc cacgacgcag cgacctgtgc cggaatttcc
    19381 agactgtcgt actggccttt ctccaccacg cgggcgtagt tgttcagccc agactgcgtc
    19441 tcttctttat cgcctttttt caccgtaaac agcatctcgc cgctctcggt gttgtgggct
    19501 ttgtcgcggg tcgcgccata ttccggcacc ccgttgccgt tatgatcgcg gttacgtaac
    19561 caccagtcgt gataggccac cagtttcggg tacatctctg ccaaccaggc tttatcctgg
    19621 gtgacgttgt atacttccat caccgaccag gcggcaaggc tgggtttggt attacgttcg
    19681 ttccagttgc cgccatcgcc accacgctcg gggctaaggt tccacgctat caggtcgggg
    19741 acaaagccca catcctgcgg acgcacgctg tcgccaggct ggatctgcca ggagaagacc
    19801 gcgcggatat tctctttggc gatatccgga ttgaaatgcg ccatcgcaaa cgcctgcttc
    19861 caggtatccc acggccaggt ctgattgccg gagaaccagc gtccggtcac cgacggcgtg
    19921 acggtgttat aattcaccgc accgcccggt gagcgccagt taccgttgag cgtttcgatc
    19981 gctttcaccg cgacgcgcgt ctgttccggc gtcgcatccg gattggttaa ccctttcttc
    20041 aggtattctt cccagcgttg ctgcgaggcg gtgagataaa acgccggacg cgccagaata
    20101 tcgcggatct gcatttgctc tttgctaact tcctgagcgg tcagcagatg ggaataggtg
    20161 gtgtagagcg tggtcgaacc gttgatatgc gccttactgg taaagcgatt accgttgatt
    20221 tcagtctgca ccggcaggga tttatgcacc tgatattccg attcgccgga ggtcagcaga
    20281 tcccaggtgg cgcgcacttt gccaaaggtg actttcaggc catcacgggt ggcgctgatt
    20341 ttgcgctgat agtcagggta ttcgccagca atggttttat cggaaagcgg tttcccttct
    20401 ttcgcttcca gtttttccag cagttcgcca tcccacacca gatccagcgg tttattgctg
    20461 atgattttag tttccagtag tgacgtgcgc ggcgtggcga agcgcagagt catttcgacc
    20521 tgcacatctt ttgctgtcag tttttgcacc agcgcgccgg gaatactgta tgcctccagc
    20581 gtgaagttga ctttcttgcc gtcttgccag acagtcaggc ggtcgaaatt gctggccata
    20641 aagttgatgt attcttccgt cagcagcgca acgcccggaa agccgcccat ggtgttcggg
    20701 ccgtctggca acagatgacc atgccaggct ccgagatcga aaaacggatt aaaacgctgg
    20761 tgatcgtcgt aatcgtaatc cttcatgtac tgcggcgcac cagtacggtt aatcacgttt
    20821 ttataattgt cggcgttagc ggcatgagcg gaaaaactta tcagcagagc gcaggttact
    20881 ggcgttaaaa tagttttgat tttcatgaga ttacagtcct tattaccagt agaaatattg
    20941 catcaggaag atttggtcgc tgggatcgtt agaggcattt ttcatgtaga agcggctatc
    21001 aaaagccgtg gcgaaacgtc cgccgccata ttcgtaccag ataccaaact gcatgaaaga
    21061 ggtggtttca tcgtcagcgt ggtcagggcg atgtttggcg taggtgtaag cggtggagag
    21121 ataaacgcct tttaacgctt catacatggc gctcaccatc atcccgcttt cacttcccgg
    21181 tacgtccagc ccgtcggcac gtccggtatg gtgccaggcg cgagcggcaa agttaggggt
    21241 aaacaggccc gtgagataga gttgtcctgt gccgtcggta atttccagac cattcatcca
    21301 ggtgatgcct tcggcaaggt catactggat gtaaccgttc atccattgcc ggataggtgt
    21361 atttgtcgtc gtagcgatcg tacttgccaa aatgtagcca ggctttgctt tcgtcatgat
    21421 gtccggcagg tgtggcggtg acgctataac gaaagttgcc gcttaagttc tgtaccttca
    21481 gcgcggtcat aatgtcgcgg gtattgggaa tgacgtagcc caaatcgggt gtgaagtcgc
    21541 cccaccattg caggtcatcc agcgaggaat cctgacgcag actgagcatg atttcggtgc
    21601 catcattggt acgataaccg ccatagaaac ggttaatccc accttcgaaa ccaccccagc
    21661 tatggtcagg cacctaggca ttaccgtcgt ggtcggcctg cacggtccag ccttcgccgt
    21721 aaatcaggcc gaaccaggag ccgtacttca tctccaggcc accttcaata taggtgccgt
    21781 cgctgtattt atgtttattc tcaccctcta aatccatata tccaccaagc cctaattcgc
    21841 cgtaaaaacg aaatgaagga gaagatgtcg aggtaattgc gggtgttttt gtgtcgtcat
    21901 gttctgccgg acgtgcttct tctcctgccg cagaaaaggc gtagcaaaac ggcagagcaa
    21961 gtaatgctct gcatggtgca gtgattaatt tcattgtgtt aatccatgag ctaaagaaaa
    22021 agtacagcct tttatcgttg taattataaa aatataaagg cagaaactat tttccctctc
    22081 ctcatccagg aagagggaaa atgcctgctc attttttatt agtctgctta atcaacattg
    22141 ctggctggcg tcgcttcaat atggcgctct tcagccgtta atccctttat atatttgctg
    22201 tatttccacc acgcaaagcc gaggaagata acaataccgc cgacgttata aaaaatgatg
    22261 gtcagaatat tcgcgccagt cgggaatgtc gaagcgacaa accctacggc aaatatcgca
    22321 atcagcattg aaacaacgat aataccggtg cggcgggagc ccatacggaa atcgcgtggc
    22381 aggtgatcta atttggcgcg taaattcagg taagccagca tgatgaataa cggcggaagc
    22441 atggacgctg cggcggtcat attaataatg gtattcatca gatcctgcac ggtattggaa
    22501 ccgagcatcg ggataatcat cagcgggatg acgatcagaa actggatcca tgctgcgcgc
    22561 gccggaacgc cgttttcgtt aagttcgacg gttttcttac caaagatgcc ttccgggatt
    22621 tcggagaaga aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacattgcg
    22681 gtaaaggaca ccagaccgac aaagcgattc atcaacgctt ccggcagacc aaaatacgcc
    22741 gccatgccgt ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg
    22801 aagacgttaa tcagcaccga ggagacggaa tacagtacgc cgataaaaat cccggcgagg
    22861 atgatcactt taacgaacga tttcgaaccg cctttgacgt cgttaacgta caccgcgacg
    22921 gactccgcgc cacctgcggc cataaagatc caggtggtaa cgccgaggaa cgcccagttg
    22981 aagttcggga tcatcgcgtc aacggtgatc ggatcggcag gctgtacgcc gccaaccagt
    23041 gccgtacctg ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg
    23101 gaggtaattg gccccagcat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac
    23161 agcaccatac tgataacggt ggtggcaacc ggcgtcataa tatattcgta gccgaggaag
    23221 gcatacgaag cataagcaat aacgcgcggc aacagcgagg tgaaaaagaa cagatttacg
    23281 aaccagtaag tgtaggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccac
    23341 gcgtagacac cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag
    23401 aagggaataa aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaagc
    23461 tcaatattat tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc
    23521 aagccgaatt tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatgt
    23581 gtagggtagc ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat
    23641 aaccatcttc gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc
    23701 gatacgcttc atggatatcg caaataacga tttgcccttc gtggacctct acggtttctc
    23761 cgcagccttt taaatattca cggtcagttt catcacgata atattccact acctgtaatg
    23821 tctctttcgg cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat
    23881 agcgtcgatg cccggtaaat agcgcatcgg tcgcggagtc attttccaca cggtaggtca
    23941 atgaatcacc gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt
    24001 caacgcagcg ttgccacttc ttgccagagg cgtaaatctg gcggaactgt tctaagttat
    24061 cgatgatcct cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa
    24121 ctcatacgac gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa
    24181 gccgtagctg aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga
    24241 gttggagccg aggccaagta gctggtggtc gaggttcagg gtgatgtcat cactgcgctg
    24301 cagctcgtta cagtgctggg cagcgtggat gttttcctgg gtatagtgcc aggcgctgaa
    24361 gttaattggg cgctgcggaa ccaccagcag accgttgccg tggcggttag tcagtgccgt
    24421 ccagcggaca tgctgacggt tgccgttgtt ctgcgggaag ggatagttct cgaacatggc
    24481 atcgacggtg ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc
    24541 gcccggtcca cgaccgtaat acgccacctg atcgtattcg ccgttaatcc ccatggtgaa
    24601 accgatgcac ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt
    24661 aacctggcca tcggcagcga tgcgccagat gtaggtacag cgcatcccga agtcaaacac
    24721 cggcggtgca ataactgtgc ggctgatgat caacacttca ccatcgctct gttctacggc
    24781 aaagtcgcgc agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta
    24841 ctcctgcttg tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag
    24901 cagcgactcg ccatttacct gccaggatgt cggtttgcca ctcatttttg agaaggtgat
    24961 cgcgaagttg tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg
    25021 cgcattattt ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata
    25081 agtggcgata gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat
    25141 gttgaggaac gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct
    25201 gttcggcgca acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg
    25261 cacctctgcg tgcagggtgt agtcatcaag cgtggtaaac cacagtttat tttcgacttt
    25321 caactcgccg cgagtcagcg cgtggatttt taccggcgcg ataacctgtt tgtactcttt
    25381 caggcccgat cccggcgtct gatcggaata gatcaaacca tcaagacaga agttatagtt
    25441 gttgggatag tcgccgtagt cgccgccgaa tttataccag acattgccgt ggtcgtcctg
    25501 tgcctggatc ccgtggtcgc accactccca gacataatga ccctgaatgc aatcgtgctt
    25561 atagaagacg ttctggtact ccgtcagccc gcccggtccg ttccccatcg catgagcata
    25621 ttcacagatg atgcgcggct tcggatgcgg gtattcacca aactcattca tcagcggcac
    25681 gcgggtgtac atggtggaaa taatatcgac cacttcagca tcgcgatctt cttcgtaatg
    25741 aaccagtcgc gtgtcatcca gcgctttcgc cgcatggtac atcgcgcgga tgttacagcc
    25801 atagccggat tcattgccca gcgaccagat gatgatcgac ggatggtttt tctgtgcgtg
    25861 gatatggcga acaatgcgct cgacgtagac tttttcccac tgcggatcgt cggtaatacg
    25921 gctgatatcg ccgacattag caaagccgtg cgattcgacg tcggtttccg ccatcacaaa
    25981 caggccgtag atatcacaca gttcgtaaaa acgcggatcg ttcgggtagt gagcggtacg
    26041 cacggagttg atattgtgct gcttcatcaa ctggagatct ttctcgacgc gatccattcc
    26101 aacggcgcgg cctttgcgat gatcgttgtc gtgacggttg acgccgtgca gcatcacata
    26161 acggttattg atccagaaca gaccgtcgcg cactttgata tcacggaagc caacgcgttg
    26221 tggcaccact tccagaacgt tgccgttggc gtctttcagc gtcatgacca gatggtaaag
    26281 ataaggggat tctgctgacc attgctgcgg ctgttcgaca gtaaaagcaa agctggcgct
    26341 ggtcagtttt tcaattgcca aatgatcaat ggcgctgctg tgcaccacgc gttcgccatc
    26401 aaacagggta tattccagcg tcgtgacgac aggggaggcg gcgagatttt ccagcaccac
    26461 ttcgcaggaa agcgtggcat cgcaataggc ttcgtcaaag tcggtacgca cagtgaaatc
    26521 gttaatatgc gttaggtgtt ttccgaccag ataaacatcg cggaagatcc ccgccgacca
    26581 ccacatatcc tggtcttcca cgtaggtaga gtccgcccac tgcatcacgc gcacacacaa
    26641 caggttgtcg ccggttttaa ccatcgcgct gatgtcaaac tctgcggtca ggcgactgcc
    26701 cttgctgaaa cccacatact gaccgttaac gtagacttca aaataggttt cgacgccgtc
    26761 aaatttaatc agcgtctgtt taccctgcca gccgtcgctg agggtgaaaa tacgttgata
    26821 ggcaccggtt gggttatcgc tggggacaaa cggcacatcg atggggaacg gaaaaccttc
    26881 gtcggtatat tgcagtttgc cgtgaccttc catttgccac atggcgggga cggtaatatg
    26941 cccccagtca gccattaact cagaggtgaa ggcttctggt acttgcagcg gatggtcaaa
    27001 aaagtggaaa ttccactgac tgcttaaggg cagaaacagg ctgctggttt cgcgggcaaa
    27061 ggtacgcgct tgcgcaacag aatcatatga aaaaaagtac gcacgcggcg caagtcggtt
    27121 ttcgtgggtg agctgaatgt tttcccagcg attcataggg cctcccggta gagagaaatc
    27181 ggaaccattc atggtgatgg tagtttgtgt aattgctggg ggtttagtaa aatttttact
    27241 aaaaagcagc aggtaaatgc atttacttta actggatcac gaaaataaac ggcagcactg
    27301 tccggcaaaa atgattgccg aacagtgtgt aaggggattt aacgggtcgt gccgcgcagt
    27361 tttaatttgc tgggaacgaa gactagcagc ggcagcgcgc gaccatcgcg ggctttttca
    27421 tacaccaggt taacgccctg acttcccatc atctcggaat ggatgcgcac ggtggagagc
    27481 ggcggaaagg taaatcgcgc ggtggggata tcgttaacgc tgataagcga aatatcttgt
    27541 gggatgttca ggccacgttc atgaattgcc cgcagtacgc cgatagcaat ggaatcggaa
    27601 gcaacaaaca gtgccttcgg atagtcttcc cgcgccagca tttgttttgc cagttcataa
    27661 cccgacgaac tggaaaaacc gccgcgccag atatcctctt cgcgtaccac ttgtttcagt
    27721 cggccatatt ccgcaaaggc gacctcacga atatccgcct tgccaggctc atcttcaccg
    27781 ccaataaaac caatacgatt aacgccctgg ttgatataga agtcgatgat ttctttactg
    27841 atgcgtgcca gatcgatatc caccgcatcg taaccgctgc cgggttcgtg aaagtcgata
    27901 aaacagatat tgtcggtcaa cgcgctggca gcggcgcgca gggcgggcgt gggtttgccg
    27961 acaattaaaa taccggtgac gtttttaatg tctggtaatc cgttgtgttc ataacagttg
    28021 gtcagttcga tcgccagctt ttcgcactga gtttcaatgc cgtggcggat cgccagatag
    28081 taaggatcgt tgatctccag ctcctgctgg tagctgtaga tagccagaat atggtgttgg
    28141 ttgactgcgc ctgtctgtag tttacgggcg ctactggtct tgtactccag cttttcggcg
    28201 atctcgagaa tgcgatgttt cgtctcttct ttcacattca atgtcggatc gtcatttaag
    28261 accctggata ctgtcgccag ggatacgcca gcttcgattg cgatgtcttt taatgttgcc
    28321 atgtttacct tttttacttt ccctgatgtg cgcccccatt gtagaggaag tgcttatcgg
    28381 gtcacctgtt ttcagtaaat aaagcgcgtt tctttgacgc tgtaacggta acacagcggc
    28441 ttaggaatgt gatcacgcct gcattctcca tttttagcct acaggattga gaaaaacttt
    28501 tctatagttt agtaaaattt tactcgaagg gaggaagcaa tggaaaccgt ggcttacgct
    28561 gattttgcac gtctggaaat gcgcgtcgga aagattgtgg aagtgaaacg ccatgaaaac
    28621 gccgacaagc tgtacatcgt acaggttgat gtggggaaaa aacactgcaa accgtgacca
    28681 gcctggtgcc gtactacagc gaagaggagc tgatggggaa aacggtggtg gtcttatgca
    28741 atctgcaaaa agcgaaaatg cgtggtgaaa cgtcggaatg catgctgttg tgtgcggaaa
    28801 ccgatgacgg cagtgaaagc gtgttgttaa cgccggagcg gatgatgcct gcgggcgtac
    28861 gcgtcgtata aaaagatcgg atggcgacgt cgtatcgcca tccgatttga tattacgctt
    28921 cttcgacact tactcgcatg gccgccagcg ccttacgcgc cgctttgatc accagttcac
    28981 actgttcaat ggtcagtgtc agtggcggtt caatgcggat cgttttggcg ttattgagcg
    29041 ttccggccac cagtacgcgc tggcggaaca tctcgctggc aaagttatag ccgatttcgt
    29101 tatcaacaaa ctcaatcgcc atcaacatcc ctttaccacg cgcttcctgt accagatcgg
    29161 gatattcccg cgccagttga cggaaaccgt ccagcaacat atcgcctttt tgctcagcct
    29221 gagccggtaa gttctgctcc agcaacacat tgatggtcgc cagcgccgcc gcacaggcca
    29281 gcgggttgcc gccaaaggtg gtggtatgca ggaatgggtt gtcgaacaga actgaaaaca
    29341 cctcttcagt ggcgatggtc gcgccaatcg gcatcacgcc gccgccgagg gcttttgcca
    29401 gacacagaat gtccggctga acgttctcat gttcgcaggc aaacatcttg cccgtgcgcc
    29461 ccatgcccgt ttgtacttca tcgaggatca tcagtgcgcc gaactcatcg cacagcttac
    29521 gcacggcggt gagatagccc ggcggcggca gaattacgcc accttcgccc tgaatcggtt
    29581 cgaggatcac cgcagccaca tcatcaccgg tttttttgca ctcattaaga gccgtgcgca
    29641 tggcttcgat attgccaaac ggcacatgac ggaaccctgg cagcagtggc ataaacggtt
    29701 tgcggaaggt cgatttcgcc gtggccgaca gcgcgccaag tgatttaccg tggaacacgc
    29761 cgctggtggc aataaaagta aatttgccgc gcggcgactg ataagccttc gccagcttca
    29821 gcgccgcttc aacggactcg gtgccgctat tacagaagaa gctgtatttc agtttaccgg
    29881 gcgttagcgc agcaagggtt ttcgccagca tagcccgtaa cggatcgagt agctcctggc
    29941 tgtgcagtgg ttgtttcgca agttgattct gtacggcgga aaccacaact ggattacggt
    30001 gccccacgtt gaaaattcca aaacctccca ggcagtcgat aaactcctgt ccctgggtgt
    30061 cgacaagcgt atttaaacct cccgcttgcc actctacggc tccgtaatcc ccgccggcgg
    30121 taacagattt gcgatactct aaaaaccccg gattgacatg ctctttgaag tattcaatca
    30181 cctctcggtt aagtgctttc atctcctcat gatccagcgt tcgcttctca atgagattca
    30241 gggcgtgggc gctgcacgct aaagccgatg cgctcgaagg taacctgttc aaaatatgct
    30301 ccggaggctc gcgtatcaca tgataccgat ttaagtattg cagggattgc gccactccgg
    30361 ctgcgatcgc gaaaatcggg ataaacacaa atgcggttat catttgtgca atatttaatg
    30421 ctatccgtta acatttgttg ttattttgca cggtttttgt cgggtatgga tttgatgatt
    30481 gctgcgcaaa tgcacgtttt cagggcaata attgcctgat ttttgggcaa gataattaac
    30541 gttttcgtat agtaacagag tgaaagtatt ttaaatcaaa gaattagatt tgtgctttaa
    30601 tcgcaaattg cgatctaaat caaattaatc ggttaaagat aaccgcagcg gggccgacat
    30661 aaactctgac aagaagttaa caaccatata acctgcacag gacgcgaaca tgtcttctca
    30721 tccgtatgtc acccagcaaa ataccccgct ggcggacgat accactctga tgtccactac
    30781 cgatctgcaa agctatatca ctcatgctaa tgacactttt gtgcaggtga gcggctttac
    30841 cttgcaagag ttacaagggc agccgcacaa catggtgcgt cacccggata tgccaaaagc
    30901 ggcgtttgcg gatatgtggt tcaccctgaa aaaaggggag ccctggagcg gcatcgtgaa
    30961 aaatcgccgc aaaaatggcg accattattg ggtgcgggcc aatgcggtac cgatggtgcg
    31021 cgagggaaaa atcagtggct atatgtcgat tcgtacccgg gcgacggatg aagagattgc
    31081 ggcggtggag ccgctgtaca aagcgttgaa ctccggacgt accagtaagc gtattcataa
    31141 aggcctggtg gtgcgtaaag gctggctggg taaactgcct tcattaccgc ttcgctggcg
    31201 gacgcgtgga gtgatgaccc tgatgtttat cttgctggcg gccatgcttt ggtttgttgc
    31261 tgccccggtg gtgacgtatt tcctctgtgt gttagtggta ttgttggcaa gcgcttgttt
    31321 tgaatggcag attgtgcgcc cgatagaaaa tgtcgcccgt caggcactga aggtggcgac
    31381 cggagagcgt aatagtgttg agcacctgaa tcgcagcgat gagctggggc tgacattacg
    31441 cgcggtaggg cagcttggcc tgatgtgccg ttggctaatt aacgatgtct caagccaggt
    31501 gtccagtgtc agaaatggca gtgagacgct ggcgaaaggc accgatgaac tgaacgaaca
    31561 tacccagcag acagttgata acgttcagca aacggtggcg accatgaacc aaatggcggc
    31621 gtcggtgaaa cagaactctg ccacggcgtc ggctgccgat aaactttcta tcaccgccag
    31681 taatgcggca gtgcagggtg gggaggcgat gaccacggtg atcaagacaa tggacgatat
    31741 cgccgacagt acctagcgca ttggcaccat tacttcgctg attaacgata ttgcgtttca
    31801 gaccaatatt ctggccctga atgcggcggt ggaagcggcg cgtgccggcg aacagggcaa
    31861 aggttttgca gtggtggcag gggaagtgcg tcatttagcc agccgcagcg ctaatgctgc
    31921 caacgatatt cgcaagctga ttgatgccag tgctgataag gtgcaatccg gttcgcagca
    31981 ggtacacgcc gccggacgga cgatggaaga tattgtggca caggtgaaaa acgtcaccca
    32041 gttgatcgcc cagattagcc attcaacgct ggaacaggcc gatgggcttt ccagcctgac
    32101 ccgtgcagtg gatgagctta acctgatcac ccagaaaaat gccgagctgg tggaagagag
    32161 tgcgcaggtg tcggcgatgg tgaaacaccg cgccagccga ctggaagacg cggtgacggt
    32221 gctgcattaa tcggtatgcc ggatccggcg gttggagcac aatgcctgat gcgatgctgg
    32281 cgcatcttat caggcctgcg gggtgtgtag cgggtgtagg ccggataagg cgcgtcaagc
    32341 gtcgcatccg gcaattgcac cgcgccactg gcggatgcgg cgattaatcc agttgcgtaa
    32401 tatcaaaagc ggcgcggtcg atcaccgcaa tgatcttttt tatttgtgca tcactgatat
    32461 cgctctggtt gacgcgtaaa tccagcaccg ctttaaaatt atccagcgcc cgcttcattt
    32521 gcgggttctg gcgcagcgcc gcgccaacgc aacgcgcttt gatgcgttct tcaatcatct
    32581 ccacttgttc gcggttttct tccagccact gcgcgccttg ttcggtcagc gcaatctgct
    32641 tcttaccacc ttcctcttcg cggatggtaa tcagcgactg ctcctgcaga aaatccagcg
    32701 tcgggtagat gacgcccggg cttggggtgt aattcccctg ggttagattc tcaatcgctt
    32761 taatcaattc gtaaccgtgg ctgtcatcgc gcgagagaat atccagaatc accagacgca
    32821 attcaccgtg accaaagaaa cgttgccgac gaccgccgcc gcgaccgtgg cgaccaccgc
    32881 aacattgacc gtgttcatgc tggtgaccgt gtccgcagtg ctcgtgttct gacttctcac
    32941 ctttgcagca gccctcatgg cgtggctggc cttcatgttt acaacaccct tcgtgatgat
    33001 ggctcatatc agcctccttt tgcttgatta gatatatcta atttatatct gatgtgagca
    33061 ttactgcaag tacaaaaatg atttttaaac gtggaagcac ctcacatcta ggccggataa
    33121 gacgttcacg ccgcatccgg cagcattgct atggcactga atgccttcac atacttcaga
    33181 catatcagtt ctacaaatcg cttgcattta tcatgattaa caatcattat catttgcgaa
    33241 ttttatttag atatatctga ttaccatcac gaaggcgata acaatgaata acaccccccg
    33301 ctacccgcag cgcgttcgca atgatctgcg cttccgtgaa ctgactgtgc tacgcgttga
    33361 gcgcatcagc gccggttttc agcgcattgt ccttggcggc gaggcgttgg acggttttac
    33421 atcgcgcggc tttgacgatc acagcaaact cttctttcct caacctgacg ctcactttgt
    33481 gccgccaacg gtaacggaag agagcatcgt ctggccggaa ggaccacgcc caccgtcgcg
    33541 tgactatacg ccactgtatg acgaactacg ccatgaactg gcgatcgatt tctttattca
    33601 cgacggtggg gtcgccagcg gctgggcgat aaacttacgg tggcaggtcc gcgcggttcg
    33661 ctggtggtgc cggaagatta cgcgtatcag ctgtatgtct gcgatgaatc cggaatgccc
    33721 gcactgcgcc gccgcctgga aacgttgagc aaacttgccg ttaaaccgca agttagtgcg
    33781 ctggttagcg tgcgggataa cgcctgtcag gattatctcg cgcaccttga tggttttaat
    33841 atcgaatggc tggcacatga tgagcaggcg gtagatgcgc gtctggcgca gatgcaaatc
    33901 cctgccgatg attacttcat ctggataacc ggcgaaggta aagtcgttaa gaatttaagc
    33961 cgccgctttg aagcggaaca gtatgaccca cagcgggtac gtgcagcggc ttactggcac
    34021 gcaaagtaac tgatatataa aaagaaaaag gctgacgatt tctcgtcagc ctttgctata
    34081 tctggtggcc cctgctggac ttgaaccagc gaccaagcga ttatgagtcg cctgctctaa
    34141 ccactgagct aaggggccat ggtagcggat tataaagtaa ctccgtgccg caatccagcc
    34201 attaccgcgc gcctgctgtt tttataaaca atgtattttc aatccgttat acttttcctg
    34261 gtgatgttag aaaggagtaa atatggttga ggatattttg gctccagggt tacgggtcgt
    34321 gttttgcggt atcaaccctg ggctttcatc cgccgggact ggttttccct ttgctcatcc
    34381 ggcaaatcgc ttctggaagg tgatatatca ggccgggttt accgaccgtc agttgaagcc
    34441 gcaggaggca cagcatctgc tggattatcg ttgtggcatc accaaactgg tagaccgtcc
    34501 aacggtgcaa gccaatgaag tttcaaagca ggagctgcac gcaggtgggc gtaagctgat
    34561 tgaaaaaatt gaagattatc agccgcaggc gttggctatt ctgggcaaac aagcatatga
    34621 acagggattc agctagcgcg gtgcacagtg ggggaaacaa acgctcacca ttggttcgac
    34681 gcagatttgg gtgctgccaa atcccagcgg tttaagtcgc gtttcactgg agaaactggt
    34741 tgaagcgtat cgcgagctgg accaggcgct ggtagtgcgt gggcgataaa aaacgtcacc
    34801 gg
//
LOCUS       DAJTYM010000033        34703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTYM010000033 DAJTYM010000000
VERSION     DAJTYM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(24..100)
                     /locus_tag="OO575_001652"
     tRNA            complement(24..100)
                     /locus_tag="OO575_001652"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64..66),aa:Arg,seq:ccg)
     gene            complement(203..1588)
                     /locus_tag="OO575_001653"
     CDS             complement(203..1588)
                     /locus_tag="OO575_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6664654.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1779..2519)
                     /gene="rffM"
                     /locus_tag="OO575_001654"
     CDS             complement(1779..2519)
                     /gene="rffM"
                     /locus_tag="OO575_001654"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6664655.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFECIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2522..3874)
                     /gene="wzyE"
                     /locus_tag="OO575_001655"
     CDS             complement(2522..3874)
                     /gene="wzyE"
                     /locus_tag="OO575_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6664656.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3871..4950)
                     /gene="rffT"
                     /locus_tag="OO575_001656"
                     /gene_synonym="wecF"
     CDS             complement(3871..4950)
                     /gene="rffT"
                     /locus_tag="OO575_001656"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6664657.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(4947..6197)
                     /gene="wzxE"
                     /locus_tag="OO575_001657"
     CDS             complement(4947..6197)
                     /gene="wzxE"
                     /locus_tag="OO575_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6664658.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6199..7329)
                     /gene="rffA"
                     /locus_tag="OO575_001658"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6199..7329)
                     /gene="rffA"
                     /locus_tag="OO575_001658"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6664659.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRLNQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7334..8008)
                     /gene="rffC"
                     /locus_tag="OO575_001659"
                     /gene_synonym="wecD"
     CDS             complement(7334..8008)
                     /gene="rffC"
                     /locus_tag="OO575_001659"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6664660.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(7986..8867)
                     /gene="rfbA"
                     /locus_tag="OO575_001660"
     CDS             complement(7986..8867)
                     /gene="rfbA"
                     /locus_tag="OO575_001660"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6664661.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(8886..9953)
                     /gene="rffG"
                     /locus_tag="OO575_001661"
     CDS             complement(8886..9953)
                     /gene="rffG"
                     /locus_tag="OO575_001661"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6664662.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(9953..11215)
                     /gene="wecC"
                     /locus_tag="OO575_001662"
     CDS             complement(9953..11215)
                     /gene="wecC"
                     /locus_tag="OO575_001662"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6664663.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11212..12342)
                     /gene="wecB"
                     /locus_tag="OO575_001663"
     CDS             complement(11212..12342)
                     /gene="wecB"
                     /locus_tag="OO575_001663"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6664664.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12398..13444)
                     /gene="wzzE"
                     /locus_tag="OO575_001664"
     CDS             complement(12398..13444)
                     /gene="wzzE"
                     /locus_tag="OO575_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6664665.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13456..14559)
                     /gene="wecA"
                     /locus_tag="OO575_001665"
     CDS             complement(13456..14559)
                     /gene="wecA"
                     /locus_tag="OO575_001665"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6664666.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14799..16058)
                     /gene="rho"
                     /locus_tag="OO575_001666"
     CDS             complement(14799..16058)
                     /gene="rho"
                     /locus_tag="OO575_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6664667.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16143..16244)
                     /gene="rhoL"
                     /locus_tag="OO575_001667"
     CDS             complement(16143..16244)
                     /gene="rhoL"
                     /locus_tag="OO575_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6664668.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16385..16714)
                     /gene="trxA"
                     /locus_tag="OO575_001668"
     CDS             complement(16385..16714)
                     /gene="trxA"
                     /locus_tag="OO575_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6664669.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16845..18110
                     /gene="rhlB"
                     /locus_tag="OO575_001669"
     CDS             16845..18110
                     /gene="rhlB"
                     /locus_tag="OO575_001669"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6664670.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18244..19728
                     /gene="gppA"
                     /locus_tag="OO575_001670"
     CDS             18244..19728
                     /gene="gppA"
                     /locus_tag="OO575_001670"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6664671.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19775..21796)
                     /gene="rep"
                     /locus_tag="OO575_001671"
     CDS             complement(19775..21796)
                     /gene="rep"
                     /locus_tag="OO575_001671"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6664672.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            21883..22164
                     /gene="ppiC"
                     /locus_tag="OO575_001672"
     CDS             21883..22164
                     /gene="ppiC"
                     /locus_tag="OO575_001672"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6664673.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22210..23685)
                     /gene="ilvC"
                     /locus_tag="OO575_001673"
     CDS             complement(22210..23685)
                     /gene="ilvC"
                     /locus_tag="OO575_001673"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6664674.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23835..24725
                     /gene="ilvY"
                     /locus_tag="OO575_001674"
     CDS             23835..24725
                     /gene="ilvY"
                     /locus_tag="OO575_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6664675.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24722..26266)
                     /gene="ilvA"
                     /locus_tag="OO575_001675"
     CDS             complement(24722..26266)
                     /gene="ilvA"
                     /locus_tag="OO575_001675"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6664676.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26269..28119)
                     /gene="ilvD"
                     /locus_tag="OO575_001676"
     CDS             complement(26269..28119)
                     /gene="ilvD"
                     /locus_tag="OO575_001676"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6664677.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28184..29113)
                     /gene="ilvE"
                     /locus_tag="OO575_001677"
     CDS             complement(28184..29113)
                     /gene="ilvE"
                     /locus_tag="OO575_001677"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6664678.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29133..29396)
                     /gene="ilvM"
                     /locus_tag="OO575_001678"
     CDS             complement(29133..29396)
                     /gene="ilvM"
                     /locus_tag="OO575_001678"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6664679.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29393..31039)
                     /locus_tag="OO575_001679"
     CDS             complement(29393..31039)
                     /locus_tag="OO575_001679"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6664680.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31042..31092)
                     /locus_tag="OO575_001680"
     CDS             complement(31042..31092)
                     /locus_tag="OO575_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664681.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31178..31276)
                     /gene="ilvL"
                     /locus_tag="OO575_001681"
     CDS             complement(31178..31276)
                     /gene="ilvL"
                     /locus_tag="OO575_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6664682.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31628..33107
                     /locus_tag="OO575_001682"
                     /pseudo
     CDS             31628..33107
                     /locus_tag="OO575_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33132..33470)
                     /gene="maoP"
                     /locus_tag="OO575_001683"
     CDS             complement(33132..33470)
                     /gene="maoP"
                     /locus_tag="OO575_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6664683.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33589..34428
                     /gene="hdfR"
                     /locus_tag="OO575_001684"
     CDS             33589..34428
                     /gene="hdfR"
                     /locus_tag="OO575_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6664684.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(34524..34599)
                     /locus_tag="OO575_001685"
     tRNA            complement(34524..34599)
                     /locus_tag="OO575_001685"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34564..34566),aa:Trp,seq:cca)
     gene            complement(34608..34684)
                     /locus_tag="OO575_001686"
     tRNA            complement(34608..34684)
                     /locus_tag="OO575_001686"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34648..34650),aa:Asp,seq:gtc)
BASE COUNT         8459 a         9495 c         8772 g         7977 t
ORIGIN      
        1 gcagctcaag cgctgggact aaatggcgcg cccgacagga ttcgaacctg agacctctgc
       61 ctccggaggg cagcgctcta tccagctgag ctacgggcgc ttagcgccgt tgcgggggtg
      121 gataatacgg acttcccacc cctctgtcta gtcccttttt aaataaattg ctcgtttggt
      181 tacggtttgt gcgttttgct gcttattcct ccagtttatg cgctttaccg tggcgattaa
      241 gcccaaaaac tttataaatc gccgtcaccg ccagcataaa gatgatgccg acaaacagcg
      301 acatacgcgt atcttcatta aagtacatgc cgatcaaaac gcaaatcagg aatgccattg
      361 ttacgtagtt ggcccacggg aacaggattg agcggaacgg atggctggca atcgccgctt
      421 tatgtgcacg ccgaaaacgc agctggctta tcaatatcac aaaccatggc accatccccg
      481 gaagcacgct ggcactgtag acgtagacaa acacacgctg cggattggga atgatgtagt
      541 tcaggcatga gccaattagc agaatagcaa tagatactgc cacacccgca accggtacgc
      601 cgtgacggga aacttttgcc attgccgccg gtaactgacg gtttttcgcc agtgcgtaga
      661 gcatacgtcc gcaactgtac atgccgctgt tacagccaga gagcgcagcc gtcagcacca
      721 caaagttgat aatgcccgcc gctgcggtaa taccgatttt ggcaaaagtc agtacgaacg
      781 ggctgccgtt gctgccaatt tcattccacg ggaagatggt gacgataacg aaaatcgcgc
      841 ctacgtagaa aatcaggatc cgccacagca ctttgcctac ggcactgcgc agcgtcacct
      901 gcggattctt cgcttcaccg gcagtaatgc caatcagctc cacgccctgg taggacgcca
      961 ccacaataca cagagcggtc aggaaccctt tccagccacc cgcaaagaaa ccgccatgct
     1021 ctgtgagatt gctaaaacca atcgactgcc cgccattgcc aaagccaaag aaaatcacgc
     1081 ccaggccaat gacaatcatc acgataatcg tggtgacttt gatcatcgcg aaccagaact
     1141 cgatttcgcc gtacaaccgt accgccgcca gattcgccaa cgccaccagc gccaccgcga
     1201 tcaatgcggg tatccactgc gccatctccg gggaccaaaa ctggacataa acgccaatgg
     1261 cggtgatttc tgagatcccc accgccatcc acataaacca gtaagaccag gcggtgagat
     1321 agccaaaaaa cgggctcata taacgatgcg cataaacggc gaacgaaccg gtaaccggtt
     1381 cgaggaacaa catttcgccc attgaacgca tgatgaaaaa aacgaacagc ccggcgatga
     1441 tataggccaa caatacggat ggcccggccc atttcagggt actggcggcc cccataaaca
     1501 ggccgacgcc aatggtgccc cccagggcga tgagttcgat atgtcgagct tccagcccac
     1561 gctgtagctc tggtttgtta tctgccataa atcctcgtgt tgtgtttgca tgctttccgg
     1621 tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa tgttttcgta attcaggaga
     1681 aatggcaaat aaagcattaa aaatttgaat gctttgtgta ataaaaaagc agacaggcga
     1741 cgaagtgacc actccgtcgc tttacaaaga gaggaaaatc agaggttgcc ggtgtagtgc
     1801 cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc ggctcggctg cgaaagcagg
     1861 cggtagagcc actccagtcc cagcgtttgc cagattttcg gtgcgcgttt tacgtgaccg
     1921 gtgaaaacat cgtaagtccc gccaacgccc atatacagcg catctggatg caccagacgg
     1981 cagtcgcgca tgaagatctc ctgctttggc gatcccatcg caacggtgac gatttgcgct
     2041 ccgctggcat gaatgcattc aaacagcgcc tgacgctgct cgggtttaaa ataaccatcc
     2101 tgactgccaa cgatattcac attccattgg ttgcgcagtt tagcttcagt ttgcgccagc
     2161 acttcaggtt taccgcccac aagaaatacc ggcgtccctt ctttgcctgc gcgcgccatc
     2221 agctcttccc agagatcggc accggcaacg cgggaaacct gcgcctgcgg gtacttttta
     2281 cgtactgaac gtacaatgct gatgccatcc gcatatttaa attcggcagc gttaattaac
     2341 tccctgacct cggcgttatc ttcaatggtc agcatttttt cggcattaat ggcaaccagc
     2401 gttccctgct taagctgccc gtcagcaaac agataatcga gggcatgctg catatcacgc
     2461 caaccaatca actgtaagcc acgcagcgta taggtcggtg ccgtggtgtt gttattcatt
     2521 gttatccttc aacctgcgtc cggagcgatg attttgtacg tttatgaatg agtccggcgc
     2581 tttcaaaaag ccagtacaac agttttgcga tcatcagaca tgcgccgaag accacgataa
     2641 aaaagactac gcgtgagaca aacgaatcca gcccttcacg tgccagcacg atcatattga
     2701 agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt atagcgatta gtctcgcggt
     2761 tgcccagctc atacagccag tcgaaccatt tgatgatcag tccaaccaca atcgccccga
     2821 gtgggatgaa caacgcgccg cccatcacca ccagtgagcc tataagcgta ggcgagatcg
     2881 ccagtccgga gtggttattc agtacttccc aggtaaagta attggctgag ttcagcacca
     2941 tactcgggcg ccccggccac agccaggaag ggataaagac atagaaatcg cggacaattg
     3001 gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa cagcaacgcc agattctccc
     3061 acggtgaaaa ggtgtcgcga gtgagataga gaaacgtata gaacgcttca tcgccgctca
     3121 cattcattcc atagcgtttt aacgccagcc agaacatgcc aacaatcccc agcacgcccg
     3181 ccgccgccag catccacaac gaaatccagc cgcgaataat gccaataaac aggaagatag
     3241 cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat catataagtc agcaagccaa
     3301 aggcgaccgt gctgacgagg aaaaacagcc acgctttgct gtcctggcgc agaaagtaga
     3361 ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt taacgccacg ccggagactt
     3421 cactggaaaa gatctgactg taggagttaa gccggaacag caaaaagccg ttgtgcatga
     3481 agaagatgcc gacgcttacc agcgcgatac ccatcaggat cacccacgta agattggtct
     3541 ccacgcggtt catggtaaac agcggacggc gcggtacatc agcaacgcgt ttgcgtaggc
     3601 gggttttgta ggtgacatag taaaccgcgt agaagcagcc cgcagaaagc aacacctgca
     3661 acaagatttc tggcggcgcg acaccaacat caaagcgaaa taccagcacg ctggtcagcg
     3721 ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa gaagacattg aagttaaagc
     3781 gcacacggcg aaactcaaac caggtcagcg tggcaataaa cagcgtgcag agcagccaga
     3841 ccacaaacag gccactgaat tgcagcagac tcatgcgacc tccccggcgg caatcgccaa
     3901 cgcccgctgc cagccttgta gatagttagg gctaaagaag gcaatggtgt ttttatccgc
     3961 cgacgccaac tggcgctgcg cttcacgcac aatatcctcg ttgagatcgt cggtagtaaa
     4021 cagcactggc aaatgctgtt ccgtcatatc ctgccagaac ggattttccc tgttaagcac
     4081 acaaggaatg cccgcctgaa tcagtaagca aagcgtacca atgccctgct ggcgggcaaa
     4141 aataaagtaa ccaagatcgc actgacgaag tagcgccaga taggcgtcaa attccagttt
     4201 ttcgctcaaa atttgcagat tttcttcgct gaataactcc agccccgcct gacgaacttc
     4261 ctcaatgtac gcttcgttat taggcggata tcccatcggc accaccactt ttaccgtatc
     4321 gccaaattgc tgatgaacgg cacgtaaagc ggcgacatgc tcattgctgc ggtcgccgga
     4381 gttccccacc agaatggtca tttttccttc acgttgccga tcgttcgcca tcgtattgag
     4441 agaagggtcc atccgcgtcg ggaagtacag cagttctccc cgcacctttg ggtgtgtttt
     4501 ggcaaaaaag ctcaaatcgc cgcgggtggc aaatacacag ccgactcgct tttgcgccag
     4561 gcgacgtagt gggtaaaaaa gcttatatct caagccactg gaaagctcat acaggtctgc
     4621 cccccagata tgccagaaaa actggctggg cttaatgcca ccactcagca gagccagcca
     4681 cagtgtggga ttgaactgac cgtggaaaaa aaatcgctgc tgacggttag cttttgcttt
     4741 cgcgatgacc gcttccgcca gcgatttttt cccagggaaa aattgcacag aaagcgccgg
     4801 acagctatca cttaagccgt cgtccttgcc aacaaccata aactcgcgcg cgtgctcgct
     4861 cgtcgcggcc agcgcgtcat tgaaaaaccg caaaacggtt cggttatggt gagggatatc
     4921 cgatcccagt acgtgaatca gtacagtcat gcccgcctac gccagagtaa aaacacgcca
     4981 caacaaagag aaaaatagac gatataagtt gccatatatg cctgcgccgc gcccagtgca
     5041 ccatgcgcag ggattagcca gtgggcaaat accatcaata aagtgaactg gctgacttcc
     5101 gccagaatat aaaaccgcag tgacgctttg gcgatcacca gataaccaaa gacataagcg
     5161 cccactttta acacatcacc cactaactgc caggcaaaga gatcgcgcat agcggtaaat
     5221 ttattcgaca acagcaacca gatagcaaaa tcacgcagca accagacggt aaaactcgct
     5281 gccgccactg ccggtaagac gaatttcagc gatttaacca cttcccgggt gatatcgcgc
     5341 ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc tgaacgatgc cgtaataaat
     5401 tgcaggtagg catcggaaat actgctcacc ccttgccaga tccccacttc atcccagcta
     5461 tactgcgccg ccagcagttt acgcatcatg atgtaagcaa caggcaaggt caccgacgta
     5521 atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc ccagctgggt
     5581 ttcagatagc ttaacgggat gacaccacgt ttaatcaaca tgatggcggc aggaattacc
     5641 accagcgcgg gaatcagcgc cagacccagc aacgcccctt cataaccgcc caaacggtaa
     5701 ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa tcagcgataa cgcattacct
     5761 gcggcatcgc gaaagccttt catcagcgcc agtaacaggt tgccccaggc gatccccatt
     5821 tgcaccagcg ccactaaacg caccagcccc tgatagtcgg tattaccaaa caatccctgg
     5881 ctgattggcg cagctgccag cacaaaaacc agcgccatca gcgtagagaa accaagtacc
     5941 atcgctgatg aagtgccgac cacgcggcgc agctgttgcg gattatcatg gtactgggca
     6001 acgtatttgg ttacaccgtt aaagatgcca gccccggcaa gcacgccgag cacggtaatc
     6061 aactggcgga aatttgccgc cagcccaagc cccgccggac caaatgacac cgccagcaac
     6121 ttaccgacca gtaacccggc accaatcttg accagtgtac tggccgccgt ccacaaggac
     6181 gcttttgcca acgacatatc aggagaagta gttcaacaaa gtcgcaatta ccgtacgctg
     6241 attgacgggc gacaggttgt agaacagcgg caggcgcagc aggcgctcgc tctctttggt
     6301 ggtgtagcga tcttcaccgt ggaactcacc aaagtgttcc cccgcagggc aaccgtgcag
     6361 cggaatgtaa tgaaacaccg ccatgatttc cgcttctttc agaaagttaa tcaacgcgct
     6421 ccggtcatca atatcccgca gtttaatgta gaacatatgc gcgttctgca cgcagccatc
     6481 gggaatcgac ggcagctcga tacgcccggc tttcgccaga ggcgctaacg tatcgtagta
     6541 gttttgccac agcgccagac gttgctggtt gagacgatcc gctgcttcca gttgcgccca
     6601 caggtacgca gcttgcagat cggacatcaa atagctggag ccaatatcgc gccaggtata
     6661 tttatcgacc tgaccacgga agaactggct gcggttagtg cccttttcac ggatgatctc
     6721 ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg ccgccttcac cacccgccgt
     6781 gtagtttttg gtttcatgga agctaaagca gccaatatga ccaatggttc ccagtgcacg
     6841 ccctttgtaa gtggacatca cgccctgagc ggcatcttcc accacaaaca aattatgctt
     6901 tttcgccaac gccataatgg tgtccatttc gcaggccaca cccgcgtaat ggaccggcac
     6961 gataacgcgc gttttgtcgg tgatcgctgc ttcaatcaac gtttcgtcga tgttcatggt
     7021 gtccgggcga acatccacaa agacgatttt cgcgccacgc agcacaaagg cattggcggt
     7081 ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca ggctggatat cgagcagcag
     7141 cgccgccatc tccagcgaag cggtgcagga cggcgtcagt aacactttgg cgctgccaaa
     7201 acgttgctcc agccactgct ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc
     7261 gctacccatt gccgactgca tatagtcgag ttcggttccc accaccggcg gtgcgttaaa
     7321 tggaatcatg tgatcacctg tataaccagt acgcggtgct ttctacattc gcaccacttt
     7381 gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt cgccacccgc aaagttgttt
     7441 taccgcgagc atacgcccag tttagcgccg tttgcatcag ctcagcacct gcaccgcgtc
     7501 cagccagcag gccaattcgc gcatctgtcg cattgagttc ccgtaaagag acatagccgc
     7561 gaatatcgcc ggacgccgca cgtagaatca gacattgatg atcaaaggtg ccgcgcacgg
     7621 cattttcaat ccactgtgca taaaagcgac cgctggcgtc aggcgcatac cacggcgcac
     7681 gaaaacggct ttgcgcaaat gcggcgctag ctaactgacg taatgcggga atatcggtct
     7741 cttgtgccac gacagcaccg ctatcactga cattgttcac gggtagcgcc aaatcaactt
     7801 caccttctac cagggagaat cccagctgtt gcagggcatc cagttccccc gtatttgatg
     7861 ccgcaatttt ggcctgcacc cgtgaccatg gcgctaaggc gtctgacgtc aggagcggtg
     7921 cttcagacgt aatgcgcacg atggcgctgt taacaccaaa gaaggcgttt tcccaggtta
     7981 gtggctcaat actggcgcgg acgggcacga agtaactcca gcagatattg gccgtagcca
     8041 gttttcgcta atgaactggc agcacgcttc acaccctcgt catcgagcca gccgttacgc
     8101 caggcgatct cttccaggca ggcaatctta aagccctggc gtttttctac tgtctgcaca
     8161 aaagtgctgg cctcaatcag gctgtcgtga gtgccggtat ccagccaggc aaatccgcgc
     8221 ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc
     8281 tccagttcac cgcgctccga cggcttcacc tgctttgcgt actccacgac tttactgtcg
     8341 tagaaataaa gcccggtcac cgcccagttt gacttcggct gttttggctt ttcttcaagc
     8401 gagatagcgc ggaaattgtc gtcaaattcc accacgccaa agcgttccgg gtccatcacc
     8461 tgatagccaa aaaccgtcgc cccttccgtg cgcgccgcaa catgacgcag cttcggactg
     8521 aagccctgac cgaagaagat gttatcgccc agcaccagac aagaaggttc accattaagg
     8581 aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg ttcggcatat
     8641 tccagctgga taccgaactc actaccatcg cccagcaggc gctggaaata acctttatct
     8701 tccggcgtag tgatgatgag aatttcgcgg ataccggcca gcatcagcac cgacagcgga
     8761 tagtaaatca ttggcttatc gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc
     8821 ggatgcaatc gggtaccgga accgcccgcc aggataatac ccttcatgtt tgcctccgct
     8881 gaaaattagc ctttcagacc taaacgctca ccctgatagc tgccgtcctg cacctgcttc
     8941 caccagcttt cattagccag ataccactga accgttttac gcattccact ttcaaaggtt
     9001 tcctgcggca gccagccaag ttcacgggca attttcgaag catcaatggc ataacgcaga
     9061 tcatgccccg gacggtcagc gacaaaggtg atcaggtcac gatagtacgc cacgccgtgc
     9121 ggcttgttcg aagccagttc ttccagcagc tcgcaaatgg tttccacaac atcgagattc
     9181 ttacgctcgt tgtgaccacc aatattatag gtttcaccga ctttcccggt ggtcgccacg
     9241 caatacagcg cgcgggcgtg atcttccaca tacagccagt cacggatttg ctgcccgttg
     9301 ccatataccg gcagcggttt acccgccagc gcgttgagga tcatcagcgg gatcagtttt
     9361 tccggaaagt ggtaaggacc gtagttattc gagcagttgg tgatcagcgt cggcagaccg
     9421 taggtccgca gccaggcgcg caccagatgg tcgctgctgg cttttgacgc ggaataaggg
     9481 ctgctcggcg catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca
     9541 tatacttcgt cggtggagat atgatgaaaa cggaacgctg attttttatc ttccgtcagc
     9601 gtattccagt aagcccgcgc cgcttcaagc aatgtataag tcccgacaat gttggtttca
     9661 ataaacgctg ccgggccgtc aatagaacgg tcaacatggc tttcggctgc cagatgcatg
     9721 acacagtctg gctgatgctc agtgaatacg cgtgccagtt ctgcccgatc gcagatatca
     9781 actttctcaa aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat caggtttccg
     9841 gcgtaggtca gcttatcgac cactaccacc gcatcgctcg tttcgttgat gatataacgc
     9901 accagcgccg agccaataaa cccggcacca cctgttatca gaatttttct catcagcgcc
     9961 agactccttt ggcatcgacg acatactgct gatggacatt gtcgccattg ataactttga
    10021 actgactatg atcgaccagc atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct
    10081 gcgccagagt acaaagcccg gtcagttttt tcggcaactg gtggatgtta ggctcaacaa
    10141 ccagcgtttc gccgctgtgc cactgggcga tcagttcagc gatttccatc gccgggcttt
    10201 cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac
    10261 tggctcgttt atcggtagca gcaaggcagt cggcaaccgc cgctttcacc tgatcgataa
    10321 cccagaacgg tttgtgatcg ttaacttcgc gcgcggtacg gataagccgc gcctgctggg
    10381 ggttctgtgc cacgataaac cacggatcaa cagcaatgca gtgaccgccc acgccagggc
    10441 caggctgaag aatattaacg cgagggtgac gattcgccag gcgaatcagt tcccagacgt
    10501 taatcccctg atcggcacaa atcagcgaca attcattagc aaaagcgata ttcacatcgc
    10561 ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg cgagttagtg acgacacact
    10621 caccttcgag gaaaattttg tacaattcgc tggcgcgggc cgaacaaacc ggcgtcatac
    10681 caccaatcac gcgatcgttt ttaatcagct cgaccataac ctggcccggt aatacacgtt
    10741 ccgggcagta agcaatgttg acgtccgcct gctcgcccac ctgctgtggg aaagtcagat
    10801 ccgggcgcat ctctgctaac cattctgcca tcttctcggt tgaccccacc ggcgaggtgg
    10861 attcaaggat caccagcgcg ccttttttca gcactggcgc aatggagcga gcagccgatt
    10921 caacgtaggt catatctggc tcatgatcgc ctttaaacgg cgtaggtaca gcaatcagcc
    10981 aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt
    11041 ttactacgct cgccaaatca ggctcgacga tatggatttc gccacgattg atggtatcaa
    11101 ccgcgtgttg gttgatatcg acaccaatca cctgtttttg ccgtgaggca aacgctgctg
    11161 ctgtaggcag cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa ctcatagtga
    11221 tatccgatta ttttttaacg cttccagaat gcgagagcat gcctgaccat caccatacgg
    11281 gttatgggcg cggctcatag cttgatattc gttttcgtct tttaaaagac gcgtcacttc
    11341 ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc
    11401 cggacgctca gtggtatcgc gcatcaccag caccggtttc cccagcgaag gcgcttcttc
    11461 ctgaatgccg cctgagtcag tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg
    11521 taaatactcc tggggatcga tcagaatgac atttttcaca tgccccagaa tgcgattgac
    11581 cggctctctg acgttcgggt tgagatgcac cggatagaca atctggatgt cctggtgcgt
    11641 ggtggcgatg tctgccagcg cgtggcagat ttcttcaaag ccacgaccga aactctcacg
    11701 cctatgaccg gtcaccagaa tcatcttttt atcgggggcg ataaacgggt aatttgccgc
    11761 cagttctgaa cgcagcgtgt cgctgctcat cacctggtca cgcacccata acagtgcatc
    11821 aatgactgta ttaccggtaa tgaagattcg gctatccgca acgttttcac gcagcaagtt
    11881 ttgccgggaa gtttcggttg gagagaagtg atacatcgcc agatgcccgg tcaatgtacg
    11941 gttagcctct tccggccacg gcgaatagag agcgcccgtg cgcagaccag cctcaacgtg
    12001 accaacagga atacgctgat aaaacgccgc caggctggtt gccagcgtcg tcgtcgtatc
    12061 gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga ataggtttta gcccttccag
    12121 aatccgacag gttatctctg tcaggccctg tcctggctgc attatgttga gatcgtagtc
    12181 aggtacaatg gaaaagagtt tcagtacctg atcaagcatc tcccgatgct gcgcagtgac
    12241 gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg gagccatctt
    12301 gatggcttcc gggcgcgtac caaatacagt cagtactttc acatcgattc tcttcgaata
    12361 agcggcgagc gcctttgcgc tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta
    12421 atgcgacacc agccccgatc agccccccga caatgcccca cataatcatc aggaaggcac
    12481 gacgtgggct atcgcgtttt accggttctt ccggcgtacg caaatagcga taggtctgaa
    12541 aacgcggatc cagggttgga ccaacattca gggtgtttaa catggctcga ttctgatcat
    12601 agtcgagatc aaaggccgga ccgacggcct gtaaattttc cagtcgagcc tggagcattg
    12661 gacgcccaag caggaacatt tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac
    12721 tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg ctcaatactg ttcatccggc
    12781 ggtcgtagat ggctttcgcc acctcttcct gacgcttcac ctgagctttc atctggatgg
    12841 tgcgtgccgc ccatgcgcct ttcagctcat cattcagatg gctggctgca cgctggctgg
    12901 caaaagcaac atactgacgt aacaggttat tagcgtcagg cgcggtttcg gcaataagct
    12961 tcacgctgtc attgaccgcg cgagtaaagt ctccggggat aaactggatg ttgttaatca
    13021 tttcatccag caacgccgca tcggctttgc tgttgcccac catccgctgt ttgtaatagt
    13081 cggtttgcag ccagaactct ctgcgggtat cccacgaggc cagttgcata acaaactctt
    13141 tataggcttc gtccatgacc gatggttgat cggcagaagc catgtttgaa cggacatcca
    13201 ggttacgcaa aaattgctgc tgcgagtaat atccccccag catattcacc gttggacgat
    13261 cggtaatcgc cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc agcgcgatta
    13321 acgcaaacgc cagccccatg ccaataatcc atagcttccc agcccacaag gtacgaaaca
    13381 acccacgaat atccagttca ttttcagcgt cttcggccgg tttcccaggc attggttgtg
    13441 tcatcacatc ctcatttatt tggttaaatt ggggctgcca ccacgatttc tacgcagtct
    13501 gcgttttacg cgcttaataa agcgagcaac tttccaggca cgcttaatgc aatatccata
    13561 gaggaagaat gctagcaaaa agagcaccag catgacccac tccgggacaa aatgagaata
    13621 ttctgccagc acgccaatgg aagcgagcag tgctgcggca agggtaatca gcacaaacgc
    13681 ctgacgggaa gtaaacccgg cacgcatgat caaatggtga atatgctgac ggtcaggaga
    13741 gaatgggctc atgcctttac gcaggcgacg gtacataatc gccaccatat ccattagcgg
    13801 aatggcgatt atccacaaag cggtaaccgg gctgatggga tgggttttgc cctgggtcgt
    13861 ttcgagcagg atccagataa cggtaaaacc aatcagcgta ctgcccgcat cacccataaa
    13921 gactttgtag cggcgaccca ggataccaag gttaagcatg atgtatggca ggatggcggc
    13981 gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc
    14041 gattgctgca aacgagacgc aggacaaccc gcccagcaag ccatcaatgc catcaaccat
    14101 gttgaacgca ttaatggccg cccagacggc aaatagcgtc aggaagtaac caaacggtcc
    14161 gagcaccatc tcccaggagc caaagatata acccaggcta ctgagataaa gtttgccgaa
    14221 caccatcata acaatgccaa cagcggcctg tatggtggca cggattttta cgctgatatc
    14281 aaaacgatca tccagcgcgc caatgaaaac aagcacaccg gcacaagcga gatagagaga
    14341 tgcatgcgga atatagtaat cgacaattcc gaacgtgaag caaatccctg cgtaaaccga
    14401 aatcccccca acgagaggta tcaatccctg gtgacgtttg cggaagtttg gtttatccac
    14461 taaaccgact ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa
    14521 aatactgatg agatcagtac tcactgtcag taaattcaca atgcatgctc tcagagaaaa
    14581 ttattagcag aagtataacc acgaagacct ttattcagaa gggaaagtcc gttccgaacc
    14641 caccagccct gtgcaaaaat caaccatttg atatgcaaat tgcataacat gccagcaaat
    14701 tccaggaatt ttcctaacat cgccagcgcg acattaagat tacagatata aaagcaaaac
    14761 gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt catcatttcg
    14821 aagaaatcgt cattggtctt ggtcatggcc agtttattaa tgaggaattc cattgcatcg
    14881 atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga
    14941 gtcgtgagca gctcttcttt acgggtaccg gaacggttgt agtcgatagc cgggaagacg
    15001 cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct
    15061 tcgtaaataa cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc gataatggtc
    15121 aggctgccgc cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg acgatgaagg
    15181 gcattggcgt ccacaccacc ggtcaacact ttacctgacg ccggaacaac agtgttgtaa
    15241 gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt gtgctcaacc
    15301 aggcgtttgg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg
    15361 tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc
    15421 ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc
    15481 tgagcaatgt tctgcagcag catagtttta ccggctttcg gcggtgccac aatcagacca
    15541 cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcagttaa atcttcagta
    15601 gaaccgttac cacgttccat acgcagacga gagtttgcgt gcagcggggt taagttctca
    15661 aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc
    15721 agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca
    15781 gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg
    15841 aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtacg
    15901 ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg
    15961 tcctgcttac gcatgcgagc caggttttcc agccccatat tttcgccgag agtgatcagc
    16021 tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt
    16081 ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac
    16141 ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga
    16201 caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga
    16261 agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta
    16321 agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat
    16381 tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca
    16441 ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc
    16501 catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc
    16561 cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc
    16621 actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac
    16681 tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg
    16741 cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg
    16801 atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt
    16861 taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa
    16921 aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg
    16981 gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt
    17041 caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg aatcaaccgc
    17101 gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccacgcc gacgcggaac
    17161 cgctggcgga agctaccggc ctgaagctgg gcctggctta cggtggtgat ggctatgaca
    17221 aacagctgaa agtgctggaa agcggcgttg acattctgat tggcaccacg ggtcgtttaa
    17281 ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtagtg gtactggacg
    17341 aagccgatcg catgtacgat ctaggcttta ttaaagatat ccgctggctg ttccgccgta
    17401 tgccgcctgc aaatcagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac
    17461 gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga
    17521 aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt
    17581 tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc gccaatacca
    17641 aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt
    17701 tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg
    17761 atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga
    17821 cgcacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc
    17881 gtacaggtcg cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat
    17941 tgaatttgcc tgctattgag acctatattg gtcactcaat tccggtaagc aaatacaatc
    18001 cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgcccg cgcacaggca
    18061 atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc
    18121 atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg atgagcttac
    18181 tgtagtaagt gattcgggtg aaagaatgca gccaacacag agacagattg aaggatgaag
    18241 agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat
    18301 atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat taaacgcaaa
    18361 gtgcgtctgg ctgctggcct gaatagcgaa aatgacctct ctaatgaagc aatggagcgc
    18421 ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt
    18481 cgcgttgtcg ctacggcgac attacgactt gccgtcaatg cgggtgattt tattgccaaa
    18541 gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcgcgtctg
    18601 atttatcagg gcgttgctca caccactggt ggtgccgatc agcgcctggt ggtggatata
    18661 ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc gttgttcagc
    18721 ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa tctggggcaa
    18781 gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa
    18841 ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag
    18901 gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaattg
    18961 aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg gctgacgctg
    19021 gaacgtgcat tagtgttccc gagtggtctg gcgatcctga tcgccatttt taccgaactg
    19081 aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg
    19141 ttgcatctgg ccgtcgagca ggatattcgc agccgtacac tgcgtaatat tcaacgccgc
    19201 tttatgatcg atattgatca ggcacagcgc gtagccaaag ttgcggctaa cttcttcgat
    19261 caggtggaaa acgaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt
    19321 cagcttcatg aaatcggcct gagcgttgac ttcaaacaag ctccgcaaca cgctgcttat
    19381 ctggtgcgta acctggatct tcccggtttt acccccgcac agaagaaact gctggcgacg
    19441 ctactgctca accagactaa cccggtcgat ctctcatcgc tgcatcagca aaatgccgta
    19501 ccaccgcgcg tcgcagaaca actctgccgt ttactccgcc tggctatcat ttttgccagc
    19561 cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc
    19621 ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgctcag
    19681 gaaaaccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc
    19741 ggatgcgatg ctgacgcatc ttctccggcc ttgattattt ccctcgtttt gccgccatca
    19801 tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcactgacca
    19861 ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa aagcggctcg
    19921 gctccgggcg caccagttcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt
    19981 ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg atattatctt
    20041 catcgatact gctctggtgc ggcaaaaacc cttcttccat accgaccatg tagacataag
    20101 gaaactccag cccttttgac gcgtggagag tcatcagttg cacctgatcc agctcttctt
    20161 cactctcacc acgctccatc atgtcgcgta aagtaaagcg cgtcaccacc tgggtgagcg
    20221 tcatcggctc atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt
    20281 tgacgttctt catgcgcatt tcggcagctt tcgtgctggg cgatgtttcg tacagccagg
    20341 attcataatc catgccgtgg atcagatcgc gcaccgcggc aatcggctcc cgctccgcca
    20401 gacgctggat ttctgccaac cagtgagtga agcgggtcaa tgcttcataa ccacgtccgc
    20461 taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca
    20521 tcgcccactc acccagcttt ttcagcgtcg ccgggccaat ctctcgcttc ggcgtgttaa
    20581 cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca
    20641 agtccttgat ttcaggacga gagaaaaacg acgtaccacc agaaattttg tacgggatgc
    20701 ggttttgcat caggaatttt tcaaacaccc gcgactgata gttaccgcga taaagaatag
    20761 cgtaatcttt gtactgcgtt ttattgacga agtgatgcgc aatcagttca ccagtgacgc
    20821 gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt tagctctgtg ccgtaaccca
    20881 gttcggagaa cagacgcttt tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg
    20941 ctttcaaaat acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg
    21001 gaaaatcctg gctcagcagc accaggtttt gcggacgtgc gccgcgccag gagtagatcg
    21061 actggtcatc gtcacccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca
    21121 gttcatactg gctggtgttg gtgtcctgat attcgtccac cagcaggtag cgaattttgt
    21181 tctgccagcg ctcgcggact tcttcattgc gttgcagaag tagcgtcggc aataaaatca
    21241 gatcatcgaa gtcgagcaca ttacaggctt tcaggtgtgc atcatacagc ccataacaat
    21301 gggcaaaaat acggtcccgc tcgccaatcg cactggctgc cgcctgggac ggtgttttga
    21361 gatcgttctt ccagttagag atggtcgaaa tcaactgttg caggagaact ttgtcatctt
    21421 caatcagccc ttcggtcaac tctttgagca acgccagctg atcggtatcg tcaaacagcg
    21481 aaaagttcgc cttcatcccc agcgccgcat actcacgctt gatgatatcc agccccagcg
    21541 tgtggaaggt tgagatcatc agcccatgcg cctctttgcg acccagcgtc tgtccgacac
    21601 gctctttcat ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata tgtcgcgcct
    21661 gataaccgca cccgcggatc agatgggcga ttttattggt gataacacga gttttaccgg
    21721 aacccgcacc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc
    21781 cggggtttag acgcatagga aaatcgctca atgaaagtcg ggaggggaaa ataacagcgt
    21841 ggtagtatag ccagcctcat ccttaccact caaggcacga tcatggcaaa aacagcagca
    21901 gcactgcata tccttgtaaa agaagagaaa ctggctctgg atcttctcgt gcagattaag
    21961 aacggtgccg atttcggcaa gctggcgaag aaacactcca tttgcccatc aggcaaacgc
    22021 ggcggtgatt taggtgaatt ccgccagggt cagatggttc cggcgttcga taaagttgtg
    22081 ttctcttgtc cggtactgga gccaaccggc ccgctgcaca cccagttcgg ttaccacatc
    22141 attaaggtgc tgtaccgtaa ctaatagcaa ggccttctcc gggagaaggc cttgaactct
    22201 gaatcgctat taacccgcaa cagcaatacg tttcatatcc gtcatatagc cgcgcagttt
    22261 cttacctact tgctcaatcg catggctgcg aatcgcttca tttacatcac gcagctgcgc
    22321 gttatctacc gcaccttccg gaatagcttt acccaaatcg cccggttgca gttctgccat
    22381 aaacggtttc agcagcggca cacaagcgta agagaacaga tagttaccgt actcggcagt
    22441 atcagagata accacgttca tttcatacag acgcttacgg acgatggtgt tggcaatcag
    22501 cggcagttcg tgcagtgatt catagtaagc agattcttcg atgatgccgg aatcaaccat
    22561 ggtttcgaac gccagttcaa cgcccgcttt caccatcgcg atcatcagta cgcctttatc
    22621 gaagtactcc tgctcgccga ttttgccttc atactgcggc gcggtttcga aggcggtttt
    22681 gccggtctct tcacgccagg tcagcagttt cttatcatcg ttggcccagt ccgccatcat
    22741 accggaggag aattcgccgg agatgatgtc gtccatatgt ttctggaaca gcggtgccat
    22801 gatctctttc agctgttcag acaacgcgta agcacgcagt ttcgccgggt tagagagacg
    22861 gtccatcatc agtgtgatac cgccctgctt cagcgcttcg gtgatggttt cccaaccgaa
    22921 ctgaattagt ttttctgcgt atgccggatc ggtgccctct tccaccagct tgtcgaagca
    22981 cagcagcgag ccagcctgca acataccgca caggatggtt tgctcaccca tcaggtcaga
    23041 tttcacttcc gcaacgaagg acgattccag cacgcccgca cggtgaccac cggttgctgc
    23101 cgcccatgct ttagcgatcg ccatgccttc gcctttcgga tcgttttccg ggtgaacggc
    23161 aatcagcgtc ggtacgccga atccacgttt gtactcttca cgcacttcgg tgccagggca
    23221 tttcggcgca accatgacta cggtgatgtc tttacggatc tgctcgccca cttcgacgat
    23281 gttgaaaccg tgcgagtaac ccagcgccgc gccgtctttc atcagtggct gtacggtgcg
    23341 cactacatca gagtgctgct tgtccggcgt caggttaacc accagatccg cctgtgggat
    23401 cagttcttcg taagtaccca ctttaaaacc attttcggtc gctttacgcc aggacgcgcg
    23461 cttctcggca atcgcttctt tacgcagagc gtaggagata tcgagaccag aatcacgcat
    23521 gttcagaccc tggttcagac cttgtgcgcc acagccgacg atgactactt ttttaccctg
    23581 aaggtagctc gcgccatcgg cgaattcatc gcgccccata aagcgacatt tgcccagctg
    23641 tgccagctgc tgacgcagat tcagtgtatt gaagtagtta gccatggtga ttcctcgtga
    23701 tgttgtgctt cttattgttc ggttcgcgtt tgcgagattg aattcactat atgacaggaa
    23761 atttattgcg gaaattgata tattcacaac gtcacattgc aatttctgca acgtcaacat
    23821 tgagggctgc gccattggat ttacgcgatc tgaaaacctt cctgcatctg gcggaaagcc
    23881 gccattttgg ccgcagcgcg cgggcgatgc acgttagccc gtccacgctc tcacggcaga
    23941 ttcagcgcct ggaagaagat ctcggccagc cgctgtttgt gcgcgataac cgcacggtga
    24001 cgctgactga agcgggcgaa gaactgcgcg ttttcgccca gcaaacgctg ttgcagtatc
    24061 agcagctgcg ccacaccatc gaccagcaag ggccgttgct ctatggcgaa ttacatatct
    24121 tctgctcggt gaccgccgct tacagccatc tgccgccgat tctggatcgc ttccgcgcgg
    24181 aacacccgtc ggtggagatc aaactcacta ccggtgatgc ggcagatgca atggaaaaag
    24241 tcgtcacagg tgaagctgat ctggcgattg ccgggaagcc agagactctg cccggcgcgg
    24301 tggcgttttc gatgttggag aatctggcag tggtgttgat tgcacccgca ttgccctgcc
    24361 cggtgcgtaa tcaggtttct gtcgaaaagc cggactggtc aacggtaccg tttatcatgg
    24421 ccaatcaggg gccggtgcgc cgccgcattg agttgtggtt ccgacgcaat aaaatcagta
    24481 acccgatgat ttacgccacg gttggcgggc atgaagcgat ggtatcgatg gtggcgctcg
    24541 gctgtggcgt ggcattgttg ccggaagtgg tgctggaaaa cagccccgaa ccggtgcgta
    24601 accgcgtgat gattttagag cgcagcgatg agaaaacgcc gtttgagctg ggcgtgtgtg
    24661 cgcagaaaaa gcggctacat gagccgctaa ttgaggcatt ctggaagatt ttgccgaacc
    24721 attaacccgc caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtagc
    24781 ccagctcatt cagccgggtt tcgaaatccg gttcatggtc gccaagttcg aacgccgcca
    24841 gtacgcgccc gtagtcggtg ccatggctgc gatagtggaa caaagaaatg ttccagtgcg
    24901 tacccagcgt gttgaggaag cgcagcagcg cgcccggtga ttccgggaat tcgaagctgt
    24961 agaggcgttc ctgcaacgga tgtgacggac gcccgccgac catatagcgc acatgcaact
    25021 tcgccatttc gtcgtcggag agatccacca cgctgtagcc gccgtcgttg agcatctgca
    25081 aaatttcttt gcgctcttcg aggccgcggc tcaggcgcac gccgacaaag atgcaggcgt
    25141 ttttggcatc ggcaaaacgg tagttgaact cggtgaccga tcgcccgcca agcagttggc
    25201 agaatttgag gaagctgcct ttttcttccg gaatggtcac cgccaacaac gcttcacgct
    25261 gttcgcccag ttcgcagcgt tccgagacgt agcgcaggcc gtggaagttc acgttggcac
    25321 cggaaagaat atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata tattttttca
    25381 ttcccgccag cgccagcgcg ccagagggtt ccgccaccgc acgcacatct tcgaataaat
    25441 ccttcatcgc cgcacagatc gcatcgctat cgacggtgat gatgtcgtcg agatactcct
    25501 ggcataaacg gaaggtttcg tcaccgatgc gttttaccgc gacaccttcg gcgaacagtc
    25561 ccacgcgtgg cagatcaacc ggatgacccg catccagcgc tgctttcagg caggcggagt
    25621 cttccgcttc tacggcgatc actttgattt gcggcatcag ttgtttgatc agcaccgcta
    25681 cgcccgccgc cagaccgccg ccgccgactg gcacaaatac gcggtcgaga tgggcgtcct
    25741 gctggagcag ttccagcgcc agcgtgcctt gcccggcaat caccatcgga tggtcgaacg
    25801 gcggcaccca ggtgaacccc tgctgctgtg acagttcgat cgctttagct ttcgcttcgt
    25861 caaagttcgc gccatgaagc agcacttcgc cgccgaatcc acgtaccgca tcgactttga
    25921 tgtcggcggt ggcggttggc ataacgatca gggccttcac gcctaaccgc gcagaagaaa
    25981 acgcgacgcc ctgcgcgtgg ttacccgcag aagcagtgat cacgccgtgc gctttttgtt
    26041 cttccgtcag gcccgccatc atggcgtatg cgccgcgcag cttaaagctg tgcactggct
    26101 ggcgatcttc gcgcttcacc agaatgacgt tatcaagacg cgacgacagt ttttccattt
    26161 tttgtagcgg cgtaacctgc gccgcctcgt aaaccggcgc gcgcagcact gctcttaaat
    26221 attcggcacc ttccggagca ccggacaggg gttgcgagtc agccattatt aaccccccag
    26281 tttcgattta tcgcgcaccg cacctttgtc ggcgctggtt gccaggctgg cgtaggcgca
    26341 cagggcaaag gaaacctgac gttcacggtt tttcggcgtc caggctttgt caccccgagc
    26401 ttcctgcgct tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaatgccacg
    26461 gttcgggatg tcgatagcga tcaggtcacc atcttcaatc agaccaatgc tgccgccgct
    26521 tgccgcttcc ggtgagacgt ggccgatgga aagaccagag gtgccgccag agaaacgacc
    26581 gtcggtgatc agcgcacagg ctttaccgag acccattgat ttcaggaagc tggttgggta
    26641 gagcatttcc tgcatgccag ggccgccttt cgggccttca tagcggatca ccaccacatc
    26701 gcccgcgaca actttgccac cgaggatcgc ttctaccgcg tcgtcctggc tttcgtacac
    26761 tttcgccggg ccggtgaatt tgaggatgct gtcatcgacg cccgcggttt tcacgatgca
    26821 gccgttttca gcaaaattac cgtagagcac cgccaggccg ccgtctttgc tgtaggcgtg
    26881 ttccagcgag cggatacagc cgtttgcgcg atcgtcatcg agagaatccc aacggcaatc
    26941 ctgcgagaat gcctgtgtag tacgaatgcc cgcagggcct gcgcggaaca tattttttac
    27001 cgcgtcatcc tgggtcagca taacgtcgta ttgttccaga gtttgcggca acgtcaggcc
    27061 aagtacgttt ttcacatcac ggttcagtaa ccccgcgcga tccagttcac cgagaatacc
    27121 gataacacca ccagcacggt gaacatcttc catatggtat ttttgggtgc tcggcgcaac
    27181 tttgcataac tgcgggactt tacgcgaaag tttgtcgata tcactcatgg tgaagtcgat
    27241 ttccgcttcc tgcgccgccg ccagcaggtg aagtaccgtg ttggtagagc cgcccatcgc
    27301 gatatccagc gtcatggcat tttcaaacgc cgctttgcta gcaatattgc gcggcagtgc
    27361 actttcgtcg ttttgctcgt agtaacgttt ggtcaattca acaatgcgtt taccggcatt
    27421 gaggaacaac tgcttacggt ccgcgtgggt tgccagcagc gagccgttgc ccggctgcga
    27481 caggcccagc gcttcggtca ggcagttcat tgagttagcg gtaaacatcc cggagcagga
    27541 gccgcaggtc ggacacgcgg aacgttcaac ctgatcgctc tgggagtcag aaactttcgg
    27601 gtctgcgccc tggatcatcg catcaaccag atcgagtttg attatctgat cggacagttt
    27661 ggttttcccg gcctccatcg ggccgccgga aacaaagatc accggaatat tcaggcgcag
    27721 ggaagccatc agcatccccg gggtgatttt gtcgcagtta gagatgcaga ccatggcgtc
    27781 ggcgcagtgg gcgttgacca tatactcaac ggaatcagcg atcagttcgc gagatggaag
    27841 tgaataaagc atccccccgt ggcccatggc aatcccatca tccaccgcaa tggtgttgaa
    27901 ctctttggca acgccgccag ccgcttcaat ttgttcggcg accagtttac cgagatcgcg
    27961 cagatggacg tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt
    28021 gccaaaatcg gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt
    28081 acgaccatga gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc
    28141 tgtctcgtaa atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat
    28201 ctaaccagcc ccatttatct tcggtttcgc cagtgaagag gccgaagaag gcttgctgaa
    28261 tgcgtttggt aaccgggcca caacggcctt cgccaatctg aataccgtct acgctgcgca
    28321 ctggcgtgat ttctgccgcc gtaccggaca taaagacttc atccgccaag tacagggatt
    28381 cgcgcgacag cacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg
    28441 catcacgggt aatacccggc agcgcggagg aggtgaacgg tggggtgaac agcacaccat
    28501 ctttcacttc aaacaggttt tcgcctgcgc cttcagagat ataaccgttc acatccagcg
    28561 cgataccttc ctgataaccg tggcggcgcg cttcgctacc caccagcagg gaagagagat
    28621 agttaccacc ggcttttgcc gcagttggga tggtgtttgg tgctgcgcgg ttccaggagg
    28681 aaaccatcgc atcgatcccc tgctccagcg cttctgcgcc cagatacgct ccccacggga
    28741 aagcggcgat aatcacatcg gttgagtatc ccgctggcgg gtttactccc atgccaacat
    28801 caccgacgaa gatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt
    28861 cacgacaagc ttccatcagc tcatcaatgc tctgtgaaac cgggaagcga tagattttgg
    28921 cggagtcatg cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgtg
    28981 agtcgtagca acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca
    29041 catgcacctt cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt
    29101 tcttcgtggt catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt
    29161 gtgctctggc agatggcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt
    29221 aagtcgaccg accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg
    29281 gcggcagcca tattcattga gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca
    29341 cgttctaagg tttccggatt gaagcgagcc gatacattga cctgatgttg catcatgata
    29401 atttctccaa catttctgaa ttactggcac ctggcggcac cagcggccag acgttctcaa
    29461 gttcgtcgat tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg
    29521 ccgcttcaac ctggtcttta cgggtgatgt gttggccagg gatgccgaag gcgctggcta
    29581 acatgaggaa atcggggtta tcggtaaggg tggtttcgct gtatcgttcc tgaaaaaaca
    29641 gttgctgcca ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg
    29701 gtaactgctt gcgttttacg gtgcccagct cttgcacatt catcatgaaa gagccgtcac
    29761 cggagataca gacaacggta tcgttcggtc gcgcgacttg tgcgccaact gccgccggta
    29821 aaccaaaacc catggtacct aagccgctgg aggtgatgaa attttccggg cgagtgtggg
    29881 cgatgtgctg cgcagcccac atctggtgct gccccacatc tgtggtcacg acgcaatccg
    29941 caggtttacg atccgacagt tgttttaaca acaacggagc gtagatagcg tcaccgggat
    30001 ggtcgtaacg ccaggaatgt tcatcacgca gctgcgcgca gtgttgctgc cagtcattga
    30061 tatttaacgg ctgctgtaat gctggtaaca gagcatttaa atcaccttgt aatgccacat
    30121 gtgcctgacg cagcttgttc atttctgccg ggtcgatatc catatggata acactggcgt
    30181 gtggtgcgaa ggtgttcagt ttgccggtca cccggtcatc aaaacgtgca cccacggcga
    30241 tcagcaggtc acactcctgc accgcgaagt ttgccgcttt ggttccgtgc atccccagca
    30301 tgcccagata gtacggataa tctgcttcta ctgcgcccag ccctttcagc gtacaggtgg
    30361 caggcatttt tgtggcagcg agaaattcac gcaaagccgg aactgcctgc gccatcccca
    30421 cgccaccgcc aacgtacagc atcggttttt gcgcttttgc cagcatctgg cgcgcttgct
    30481 cgacttcggc atgtgggaaa gtcacttcgt tttcaacggt ggtgaaccac ggttccagat
    30541 cgccgctggc taactggata tcttttggga tatcgaccag aaccggacca ggacgacctg
    30601 agctggcaac gtcgaatgct tcagccatga tgcgcggcaa ctcttccaga gactgcacca
    30661 gaaagctgtg cttggtacag gctaacgaca atcccaggac atccacttcc tgaaatgcgt
    30721 cggtgccgat aaacggtgcg gacacctgac cagtgatggc aacgacaggg atggaatcta
    30781 acagtgcgtc cgcaagcccg gttatcaggt tggttgcgcc cggaccagac gtggcgatac
    30841 atacgccagt tttaccggta gcacgggcat aaccgatagc cgccattgcc gcaccctgct
    30901 catgtcggca cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa accggcataa
    30961 ttgcgccacc cggataaccg aaaacggtgt tcacaccctg tgcccgcaac gcatgtacca
    31021 cccactgtgc gccattcata gttagttccc cgtcctgaat cttgagaaac agaattttgt
    31081 gctgttattc attgtctgct cctcggttat gtttttaagg tcaaaaaaac ccccggacct
    31141 ttcggtgcgg gggtcttagt tcgttaaggc ttgatctcta agcctttcct cgtccaagtg
    31201 cagccccgca cggtgggata ataatcacca ccacgctaat cacgaccagg ctaatcactc
    31261 gtagaagggc tgtcattttg tcttttcttg catcttgttc gaaggaatgc ctaaagagtt
    31321 accatagatt ttgcgaatag cacaagatat ttttgggcct atttttatga tggctttaat
    31381 aatttcaata gaaattgttg ttttttaaaa agaaaatagt gagtgaaaaa attcaatttc
    31441 agtataaatg ttgttccctt cctcgtagtt catcctttgt ccgctgcatt tttgcgagca
    31501 tcattccacc gccagaacaa ctcaacttca cttgcacaaa taaacaggaa tcagccacta
    31561 aaattcacga attagcgggc tacagcagcg atatcttatg cacataatgg tgactcaagg
    31621 agggtttatg tcactgtcaa ttgttcatac ccgcgcagcc ctggaagtaa atgcgccccc
    31681 aatcactgtt gaggtacata tcagtaaagg tctacccggc ttaacgatgg tgggcttaac
    31741 agaaacaacg gtgaaagaag ctcgcgatcg cgtgcgcagc gccattatca atagcggata
    31801 tgaatatccg gcgaaaaaaa tcaccatcaa ccttgctcca gccgatctgc caaaagaagg
    31861 gggacgatat gatttaccta tcgccattgc gttgctggcg gcctcagaac agcttacagc
    31921 caataagtta gatgaatatg aattagtcgg agaactagcg cttacaggcg ctctgcgtgg
    31981 cgttcccggt gcaatctcca gtgcaactga agctattaag tcgggcagaa aaattatcgt
    32041 cgcgaaagat aacgaagatg aagtggggct aattaacggt gaaggatgcc tgatagccga
    32101 tcatctgcag gctgtctgtg cgtttctgga aggtaagaac actctcgaac gcccgaaacc
    32161 aactgatgca gtatcccggg cgctacaaca tgatctcagt gatgttgtcg gtcaggagca
    32221 aggaaaacga ggactggaaa ttaccgccgc tggtgggcac aaccttttac tgattgggcc
    32281 gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat ggcctgttgc cagatttaag
    32341 caatgaagag gcactggaga gcgctgcgat attaagtctg gtaaatgctg aatcagtaca
    32401 aaaacaatgg cggcagcgcc cgttccgctc acctcatcac agtgcgtcgt taactgcgat
    32461 ggtgggcggt ggtgcaattc cagggccagg tgaaatttcg ttggcgcata acggcgtgct
    32521 ttttcttgat gagctacctg aatttgaacg gcgtacactg gatgccttgc gagagccgat
    32581 tgaatccggg cagatccatc tttcacgcac acgagcaaaa ataacctatc cagcccgttt
    32641 ccagcttgtt gcggcgatga atcccagccc taccggacat tatcagggaa accataaccg
    32701 ctgcacgcca gaacagacat tacgttatct cactggagat cccattacca ccccccggca
    32761 ttttgagtaa aacggtagtg ccgggagaaa gcagcgccac cgttaaacaa cgcgtaatgg
    32821 ccgccagaga gcgccaattt aagcggcaga ataaactgaa tgcctggctg gatagtccgg
    32881 aaatacgcca attctgcaaa cttgagagcg aagatgcgat gtggttggaa gaaacgctga
    32941 tccatctggg gttatcgatt cgtgcctggc agcggttact gaaagttgca cgaaccattg
    33001 ctgatattga tcagtctgac attatcacac gtcagcattt gcaggaggca gttagctatc
    33061 gtgcgattga ccgtttgctc atccatctgc aaaaactact gacataaaaa aagggcattt
    33121 cgcccttttt attaatcgtc agaatcggtg tagtcttcag caccttcaac ctgcggttta
    33181 ccgccggaaa gggtgtgaaa acgttttgga cgcttaatac gcgtcatata cttggaccac
    33241 acgcgttctg cttctgtcac tggctcacgt tcgccacggc atactgctac gaagagtttc
    33301 tcttcctcgg taaccggctc gcgtttgcca agatccaact cattgaaggc ataaccatga
    33361 cgctcaagca gttgtgcctc tttgatggtg aaatcaccat gacgagagaa tccacgtgga
    33421 taatgtttat tgtcgaaata tcgattagtc gtcgtaaagc tttccgccat cctgcacgct
    33481 cctaattctt tgaccgagct agttatggcg cggagtatta gttacgcttg acagagtgta
    33541 aaacaaaaca tttaaatcat aacgacaaat aattttgcgg agagcactgt ggatacggaa
    33601 ttgttaaaaa ctttcctgga agttagccga acgcgtcact ttggtcgagc ggctgaatcg
    33661 ctctatctga cccagtcagc agtgagcttt cgaatcagac aactggaaaa tcaactgggt
    33721 gtgaaccttt tcacccgcca cagaaacaat atccgtttaa ccgctgccgg tgaaaaacta
    33781 ctgccttatg cagaaacgct catgagcacc tggcaggccg cccgcaagga ggtggcgcat
    33841 acctcacgac ataacgagtt ttctatcggt gccagcgcct cgttgtggga atgtatgctt
    33901 aatcagtggt tgggacgctt gtatcaaaat caggatgccc atacaggctt acagttcgaa
    33961 gcgcgaattg cccaacggca gtctctggta aaacagctgc atgaacgcca gcttgatctt
    34021 cttattacca ctgaagcgcc caaaatggac gaatttagta gtcagttgct ggggtatttc
    34081 actttagcgc tttataccag tgccccttct aaactaaagg gagatcttaa ctatctgcga
    34141 ctggagtggg ggccagattt tcaacagcat gaggcaggtt tgatcggtgc tgacgaagtt
    34201 cccattctga caaccagttc tgctgaactg gcacagcaac agattgcgat gcttaatggt
    34261 tgcacctggc tacccgtcag ctgggcgcgt aaaaaaggcg gcctgcatac cgttgtcgat
    34321 agcacaacac tttcacggcc gctttatgcc atatggctgc aaaatagcga taaaaatgcg
    34381 ttgattcgcg atcttttgaa aattaacgtg ctggatgatg tgtattaatc tgaatggctg
    34441 gcaaggatgc cggtagaagg atttacttcg gagaggatta tttcagataa aaaaaatcct
    34501 tagctttcgc taaggatgat ttctggcagg ggcggagaga ctcgaactcc caacacccgg
    34561 ttttggagac cggtgctcta ccaattgaac tacgccccta attagggtgg cggaacggac
    34621 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg
    34681 ctccaccgaa ttcttttaca acc
//
LOCUS       DAJTYM010000034        34291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTYM010000034 DAJTYM010000000
VERSION     DAJTYM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..782)
                     /locus_tag="OO575_001687"
     CDS             complement(<1..782)
                     /locus_tag="OO575_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6664685.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            874..960
                     /locus_tag="OO575_001688"
                     /pseudo
     CDS             874..960
                     /locus_tag="OO575_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1365..1841)
                     /locus_tag="OO575_001689"
     CDS             complement(1365..1841)
                     /locus_tag="OO575_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6664686.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1900..3189)
                     /gene="bioA"
                     /locus_tag="OO575_001690"
     CDS             complement(1900..3189)
                     /gene="bioA"
                     /locus_tag="OO575_001690"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6664687.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3276..4316
                     /gene="bioB"
                     /locus_tag="OO575_001691"
     CDS             3276..4316
                     /gene="bioB"
                     /locus_tag="OO575_001691"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6664688.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4313..5467
                     /gene="bioF"
                     /locus_tag="OO575_001692"
     CDS             4313..5467
                     /gene="bioF"
                     /locus_tag="OO575_001692"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6664689.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5454..6209
                     /gene="bioC"
                     /locus_tag="OO575_001693"
     CDS             5454..6209
                     /gene="bioC"
                     /locus_tag="OO575_001693"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6664690.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6202..6879
                     /gene="bioD"
                     /locus_tag="OO575_001694"
     CDS             6202..6879
                     /gene="bioD"
                     /locus_tag="OO575_001694"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6664691.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7458..9479
                     /gene="uvrB"
                     /locus_tag="OO575_001695"
     CDS             7458..9479
                     /gene="uvrB"
                     /locus_tag="OO575_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6664692.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9671..10579)
                     /gene="yvcK"
                     /locus_tag="OO575_001696"
     CDS             complement(9671..10579)
                     /gene="yvcK"
                     /locus_tag="OO575_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6664693.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10844..10988
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10976..11965
                     /gene="moaA"
                     /locus_tag="OO575_001697"
     CDS             10976..11965
                     /gene="moaA"
                     /locus_tag="OO575_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6664694.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11987..12499
                     /gene="moaB"
                     /locus_tag="OO575_001698"
     CDS             11987..12499
                     /gene="moaB"
                     /locus_tag="OO575_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6664695.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12502..12987
                     /gene="moaC"
                     /locus_tag="OO575_001699"
     CDS             12502..12987
                     /gene="moaC"
                     /locus_tag="OO575_001699"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6664696.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12980..13225
                     /gene="moaD"
                     /locus_tag="OO575_001700"
     CDS             12980..13225
                     /gene="moaD"
                     /locus_tag="OO575_001700"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6664697.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13227..13679
                     /gene="moaE"
                     /locus_tag="OO575_001701"
     CDS             13227..13679
                     /gene="moaE"
                     /locus_tag="OO575_001701"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6664698.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13816..14520
                     /locus_tag="OO575_001702"
     CDS             13816..14520
                     /locus_tag="OO575_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6664699.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14725..15438
                     /locus_tag="OO575_001703"
     CDS             14725..15438
                     /locus_tag="OO575_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6664700.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15474..16430)
                     /locus_tag="OO575_001704"
     CDS             complement(15474..16430)
                     /locus_tag="OO575_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6664701.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16430..17671)
                     /gene="clsB"
                     /locus_tag="OO575_001705"
     CDS             complement(16430..17671)
                     /gene="clsB"
                     /locus_tag="OO575_001705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6664702.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17668..18429)
                     /locus_tag="OO575_001706"
     CDS             complement(17668..18429)
                     /locus_tag="OO575_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6664703.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18562..18972
                     /locus_tag="OO575_001707"
     CDS             18562..18972
                     /locus_tag="OO575_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6664704.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18934..20040)
                     /locus_tag="OO575_001708"
     CDS             complement(18934..20040)
                     /locus_tag="OO575_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6664705.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20051..21184)
                     /locus_tag="OO575_001709"
     CDS             complement(20051..21184)
                     /locus_tag="OO575_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6664706.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21177..22913)
                     /locus_tag="OO575_001710"
     CDS             complement(21177..22913)
                     /locus_tag="OO575_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6664707.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22906..23901)
                     /gene="hlyD"
                     /locus_tag="OO575_001711"
     CDS             complement(22906..23901)
                     /gene="hlyD"
                     /locus_tag="OO575_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6664708.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23904..24575)
                     /gene="cecR"
                     /locus_tag="OO575_001712"
     CDS             complement(23904..24575)
                     /gene="cecR"
                     /locus_tag="OO575_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6664709.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24804..25991
                     /gene="rhlE"
                     /locus_tag="OO575_001713"
                     /pseudo
     CDS             24804..25991
                     /gene="rhlE"
                     /locus_tag="OO575_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26198..26680)
                     /gene="ybiA"
                     /locus_tag="OO575_001714"
     CDS             complement(26198..26680)
                     /gene="ybiA"
                     /locus_tag="OO575_001714"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6664710.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26800..28950
                     /gene="dinG"
                     /locus_tag="OO575_001715"
     CDS             26800..28950
                     /gene="dinG"
                     /locus_tag="OO575_001715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6664711.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28978..29940
                     /gene="ybiB"
                     /locus_tag="OO575_001716"
     CDS             28978..29940
                     /gene="ybiB"
                     /locus_tag="OO575_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6664712.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30081..31166
                     /gene="hcxB"
                     /locus_tag="OO575_001717"
     CDS             30081..31166
                     /gene="hcxB"
                     /locus_tag="OO575_001717"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6664713.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31395..31655)
                     /locus_tag="OO575_001718"
     CDS             complement(31395..31655)
                     /locus_tag="OO575_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6664714.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31920..32186)
                     /gene="ybiI"
                     /locus_tag="OO575_001719"
     CDS             complement(31920..32186)
                     /gene="ybiI"
                     /locus_tag="OO575_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6664715.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32260..32937)
                     /gene="ybiX"
                     /locus_tag="OO575_001720"
     CDS             complement(32260..32937)
                     /gene="ybiX"
                     /locus_tag="OO575_001720"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6664716.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32979..34291)
                     /locus_tag="OO575_001721"
                     /pseudo
     CDS             complement(32979..34291)
                     /locus_tag="OO575_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8138 a         8792 c         9249 g         8112 t
ORIGIN      
        1 cgagtcgaca atggattgtt ataaatatta acatctgcat aaatgtgcag attcatgata
       61 ctgtctggca atgtgttcag ctgattgtta gggatatata gtgattgcag acgttgtgga
      121 agttcgggca gtctgtgtag ttggttttcc ccaacgttta aaagcctcag attttgagga
      181 aggggcggta gctcttgtaa tctgttatca aaagcactta gttcctgtag ttcacatggt
      241 aattctggta aggcagtaag ctggttgttg ccaacagaaa gtttttgtag agccggggga
      301 agatcaggta gcgtctccag gctattgaga agggctgaga gtgactgcaa ggaagatgga
      361 agagaaggca aacatgttaa aactctgtta agtgaaacat caagagcgac caggtgaggc
      421 ggaagagccg ggagcctgct cagtctgtta tcgctggctt taagtacagt taaggacgga
      481 gggagttctg gcagagagcg tagctcattg ctagagatgt taagttcttg tatgtgcggg
      541 ggcaggtatg ggagagagcg taatctgagt aaacttaaat tgagggctgg ctcttgaaaa
      601 gccagacata ttttcagtaa tcgaactgcc tgtgttcggt cttctgttgc agcaccttcc
      661 ttggcccagt tatcccagat gcggtgataa tccgtgatat tttgctcttc tacggataaa
      721 cgggatatac atcctaaacc acaaaaggag cggaaaaaag aaggattgtg ggcaggtttc
      781 atttattatt gatcctcatt gtttatatat taatatattc gtgcaaatgg agtgaggatt
      841 ctttaaaaaa acgcgcctgt aaatagttaa gaacaggaag aactgttggg gatgtggctg
      901 gccgaaaatg aaggggtgaa gttctggctc aatgtgctga ctgaactgaa aaaacgcggt
      961 tggtcgccct accttttcat caagccagta gtctgcccac cacagcatca tttctctgcg
     1021 cttatcgaga tactgagcat ggttgtaaat tcagctaacc cagtgcgttg acaaaaaatt
     1081 agcgcaagag gacaaaaaat caccttgcgc taatgctctg tatcaggtca ctaataccat
     1141 ctaagtggtt gattcatagt gactggatat gttgtgtttt gcagtattat gcagtctatt
     1201 ttttaagcta aatgtattat aatgcattga tattaatgat ttttactgtt tctcggtcag
     1261 cttttttata ctaacttgag cgaaacggga aggtaaaaag acaaaaagtt gtttttaata
     1321 cctttaagtg ataccagatg gcattgcgcc atctggcaga gtgattaact aaacatcgcg
     1381 gtaatcgatg cgcttgccag agagtggaaa tgaacgttaa acccgaccat cgcgccgctg
     1441 gctccttcat cgacatcaat acgctctaca tccagcgcgt gaacggtaaa aatgtagcga
     1501 tgggtttcgc ctttcggcgg cgctgcgcca tcgtatccgg ttttaccaaa atcggtacgc
     1561 gtctgcaaaa cgccgtctgg cattgctacc agaccagagc caaacccttg cggtaatacg
     1621 cgggtatcag cgggtaaatt aacaactacc cagtgccacc agccggagcc ggttggcgca
     1681 tccgggtcgt agcaggtgac aacaaaactt ttcgttcccg caggaacatc atcccacgcc
     1741 agatgcggtg aaatattatc gccatcgtaa cccatgccgt taaagacatg acgatgcggc
     1801 aacttatcgc catcgcgcag atcgttactg atgagtttca tgaaccctcc tttcttgttt
     1861 gcagaaagtg tagccagaaa ccctcacgct gacttcccgt tattggcaaa aaaatgtttc
     1921 atcctgtacc gcgcggttaa ccgctgcggt cagacgctgc aactgttgcg ggagaataat
     1981 atagggcggc atcaggtaaa tcagtttgcc aaaaggccgg atccataccc cctgttcgac
     2041 aaagaatttt tgcagcgccg ccatattcac cggacgagtg gtttcgacca caccgattgc
     2101 ccccagtacg cgcacatcgg caaccatttc ggcatcacgg gctggtgcca gttgctcgcg
     2161 cagctgcact tcaatagccg ccacctgctg ctgccattcg ccggattcga taatcgctag
     2221 gctggcgttt gctgccgcgc aggccagcgg attgcccata aaagttggcc catgcataaa
     2281 gcagccggct tcgccgttac tgatggtttc tgcaacctcg cgcgtggtaa gtgtggcgga
     2341 aagggtcatt gtgccgccgg ttaaggcttt accgaggcac aaaatgtccg gcgcgatttc
     2401 tgcatattca caggcaaaca gtttgccggt acgaccaaat ccggtggcga tctcgtcggc
     2461 aatcagcaag ataccttcgc gatcgcacat tttgcggatt cgttttaacc attccggatg
     2521 gtacatgcgc atcccgcctg cgccctggac aatcggctca atgatcaccg ctgcgatttc
     2581 atgacgatgc gccgccatca ggcgggcaaa accaaccata tcgcgttcat cccattcacc
     2641 atccatgcgg ctttgcgggg cgggagcaaa caagttttct ggcagatagc ctttccacag
     2701 gctgtgcatt gagttgtccg gatcgcacac cgacatcgcg ccaaaggtat cgccatgata
     2761 accattgcgg aaggtcagaa aacgctggcg cgcttcgcct ttggcttgcc agtactgcaa
     2821 cgccattttc atcgccactt ccaccgctac ggaaccggag tccgcgagaa aaacgcactc
     2881 cagcggttgc ggcgtcattg ccaccagttt gcggcatagc tcgatggcgg gcgcatgggt
     2941 gataccgcca aacatcacat gcgacatggc atcaatttgc gtcttcatcg ccgcattaag
     3001 ctgcgggtga ttgtagccgt ggatcgccgc ccaccaggac gacataccgt caaccaggcg
     3061 tctgccgtca gacaaaatta gctcgcaacc ttcggcgctc gccaccggat aaaccggcag
     3121 aggggaggtc atggatgtgt atgggtgcca gatatggcgt tggtcaaagg caagatcgtc
     3181 cgttgtcata atcgacttgt aaaccaaatt gaaaagattt aggtttacaa gcctacaccg
     3241 aattaacaac aaaaaacacg ttttggagaa gccccatggc tcaccgccca cgctggacat
     3301 tgtcgcaagt cacagaatta tttgaaaaac cgttgctgga tctgctgttt gaagcgcagc
     3361 aggtgcatcg ccagcatttc gaacctcgtc aggtgcaggt cagcacgttg ctgtcgatta
     3421 agaccggagc ttgtccggaa gattgcaaat actgcccgca aagctcgcgc tacaaaaccg
     3481 ggctggaagc cgagcggttg atggaagttg aacaggtgct ggagtcggcg cgcaaagcga
     3541 aagcggcagg atcgacgcgc ttctgtatgg gcgcggcatg gaagaatccc cacgaacgcg
     3601 atatgccgta cctggaacaa atggtgcagg gggtaaaagc gatggggctg gaggcgtgta
     3661 tgacgctggg cacgttgagt gaatctcagg cgcagcgcct cgcgaacgcc gggctggatt
     3721 actacaacca caacctggac acctcgccgg agttttacgg caatatcatc accacacgca
     3781 cttatcagga acgcctcgat acgctggaaa aagtgcgcga tgccgggatc aaagtctgct
     3841 caggtggcat tgtgggctta ggcgaaacgg taaaagatcg cgccggatta ttgctgcaac
     3901 tggcaaacct gccgacgccg ccggaaagcg taccaatcaa catgctggtg aaggtgaaag
     3961 gcacgccgct tgccgataac gatgacgttg atgcctttga ttttattcgc accattgccg
     4021 tagcgcggat catgatgccg acctcttacg tgcgcctttc tgccggacgc gagcagatga
     4081 acgaacagac tcaggcgatg tgctttatgg caggcgcaaa ctcgattttc tacggttgca
     4141 aactgctgac cacgccgaat ccggaagaag ataaagacct gcaactgttc cgcaaactgg
     4201 ggctaaatcc gcagcaaact gccgtgctgg caggggataa cgaacaacag caacgtcttg
     4261 gacaggcgct gatgaccccg gacaccgacg aatattacaa cgcggcagca ttatgagctg
     4321 gcaggagaaa atcaacgcgg cgctcgatgc gcggcgtgct gccgatgccc tgcgtcgccg
     4381 ttatccggtg gcgcaaggag ccggacgctg gctggtggcg gatgatcgcc agtatctgaa
     4441 cttttccagt aacgattatt taggtttaag ccatcatccg caaattatcc gtgcctggaa
     4501 gcagggggcg gagcaatttg gcatcggtag cggcggctcc ggtcacgtca gcggttatag
     4561 cgtggtgcat caggcgctgg aagaagaact ggccgagtgg cttggctatt cgcgggcgct
     4621 gctgtttatc tctggttttg ccgcgaatca ggcagtcatt gccgcgatga tggcgaaaga
     4681 ggaccgtatt gttgccgacc ggcttagcca tgcctcattg ctggaggctg ccagtttaag
     4741 cccgtcgcag cttcgccgtt ttactcataa cgatgtcgct catctggcgc gactgcttgc
     4801 ttccccctgt ccggggcagc aactggtggt gacagaaggc gtgttcagca tggacggcga
     4861 tagcgcgcca ctggcggaaa tccagcaggt aacgcaacag cacaatggct ggttgatggt
     4921 cgatgatgcc cacggcacgg gcgttatcgg ggagcagggg cgcggcagct gctggctgca
     4981 aaaggtaaaa ccagaattgc tggtagtgac ttttggcaaa ggatttggcg tcagtggggc
     5041 tgcggtgctt tgctcaagta cggtggcgga ttatctgctg caattcgccc gccaccttat
     5101 ctacagcacc agtatgccgc ccgctcaggc gcaggcatta cgtgcgtcgc tggcggtcat
     5161 tcgccgtgat gagggggatg cacggcgcga aaaactggtg tcactcattg cgcgttttcg
     5221 tgccggagta caggatttgc cgtttacgct ggctgattca tgcagcgcca tccagccatt
     5281 gatcgtcggt gataacagcc gtgcgttaca actggcagaa aaactgcgcc agcaaggctg
     5341 ctgggtcacg gcgattcgcc cgccaaccgt acccgctggt actgcgcgac tgcgcttaac
     5401 gctaaccgct gcgcatgaaa tgcaggatat cgaccgtctg ctggaggtgc tgcatggcaa
     5461 cggttaataa acaagccatt gcagcggcat ttggtcgggc agccgcacac tatgagcaac
     5521 atgcagatct acagcgccag agtgctgacg ccttactggc aatgcttcca cagcgtaaat
     5581 acacccacgt actggacgcg ggttgtggac ctggctggat gagccgccac tggcgggaac
     5641 gtcacgcgca ggtgacggcg ttagatctct cgccgccaat gcttgcgcaa gcccgccata
     5701 aagatgccgc agatcattat ctggcaggag atatcgaatc cctgccgtta gcgactgcga
     5761 cgttcgatct tgcatggagc aatctcgcgg tgcagtggtg cggtaattta tccacggcac
     5821 tccgcgagct gtatcgggtg gtgcgccccg gaggcgtggt cgcgtttacc acgctggtgc
     5881 agggatcgtt acccgaactg catcaggcgc ggcaggcggt ggacgagcgt ccgcatgcta
     5941 atcgcttttt accgccagat gaaatcgaag agtcgctgaa cggcgtgcgt tatcaacatc
     6001 atattcagct catcacacta tggtttgatg atgcgctcag tgccatgcgt tcgctgaaag
     6061 gcatcggtgc tacgcatctt catgaagggc gcgacccgcg aatattaacg cgttcgcagt
     6121 tgcagcgatt gcaactggcc tggccgcaac aacaggggcg atatcctctg acgtatcatc
     6181 tttttttggg agtgattgct cgtgagtaaa cgttattttg tcaccggaac ggataccgaa
     6241 gtggggaaaa ccgtcgccag ttgtgcactt ttacaagccg cgaaggcagt aggttatcgg
     6301 acggcaggtt ataaaccggt cgcctctggc agcgaaaaga ccccggaagg cttacgtaat
     6361 agtgacgcgc tggcgttaca gcacaacagc agcctgcagc tggattacgc aacggtaaat
     6421 ccttacacct tcgcagaatc cacctcgccg cacatcatca gcgcccaaga gggcaggccg
     6481 atagaatcat tggtaatgag cgccggatta cgcgcgcttg aacaacaggc tgactgggtg
     6541 ttagtggaag gtgctggcgg ctggtttacg ccgctttctg acactttcac ttttgcagat
     6601 tgggtaacac aggaacaact gccggtgata ctggtagttg gtgtgaaact cggctgtatt
     6661 aatcacgcga tgttgactgc gcaggcaata caacacgccg gactgactct ggcgggttgg
     6721 gtggcgaacg atgttacgcc tccgggaaaa cgtcacgctg aatatatgac cacgctcacc
     6781 cgcatgattc ccgcgccgct gctgggagag atcccctggc ttgcagaaaa tccagaaaat
     6841 gcggcaaccg gaaagtacat aaaccttgcc ttgttgtagc cattctgtat ttggttaaat
     6901 tgcgagcgag atcgcgtctt cgattgactg caatttaacc aattaaattc taaaataatc
     6961 acgaaaaaaa ttttacttcc gcctcatgcg gcgaatgtgg gaattgccca ggcggcgggg
     7021 gataggggct ggagacagtt atccactatt cctgtggata accatgtgta ttagagttag
     7081 aaaacacgag gcaagcgaga gaatacgcgg cttgcacgcg aattggcgtt aaagacggct
     7141 caaagaaata tcttttattt tttaactggt tagataaatg caatggcagt cactgaacag
     7201 gcatctcttg ccataaaact gtcatcactc atcttgacaa atgttaaaaa agccgttgct
     7261 ttggggataa cccggtaagg ctggagtttt atctcgccac agagtaaatt ttgctcatga
     7321 ttgacagcgg agtttacgct gtatcagaaa tattatggtg atgaactgtt tttttatcca
     7381 gtataatttg ttggcataat taagtacgac gagtaaaatt acatacctgc ccgcccaact
     7441 ccttcaggta gcgactcatg agtaaaccgt tcaaactgaa ttccgctttt aaaccttctg
     7501 gcgatcagcc agaggcgatt cgacgtctcg aagaggggct ggaagatggc ctggcgcacc
     7561 agacgttact tggcgtgact ggctccggga aaaccttcac cattgccaat gtcattgctg
     7621 accttcagcg cccaaccatg gtacttgcgc ccaacaaaac gctggcggcc cagctgtatg
     7681 gcgaaatgaa agagttcttc ccggaaaacg cggtggagta tttcgtctcc tactacgact
     7741 actatcagcc ggaagcctat gtaccgagtt ccgacacctt cattgagaaa gatgcctcgg
     7801 ttaacgaaca tattgagcag atgcgtttgt ccgccaccaa agcgatgctg gagcggcgtg
     7861 atgtgattgt agtggcgtct gtttccgcga tttatggtct gggcgatcct gatttatatc
     7921 tcaagatgat gctccatctc acggtcggta tgattatcga tcagcgcgcg attctgcgcc
     7981 gactggcgga gctgcaatac gctcgtaatg atcaagcatt ccagcgtggt actttccgcg
     8041 ttcgtggcga ggtgatagat atcttcccgg cagaatcgga tgacattgca cttcgcgtgg
     8101 aactgtttga cgaggaagtg gaacgattgt cgttatttga cccgctgacc gggcagattg
     8161 tttccactat tccacgtttt accatctacc cgaaaacgca ctacgtcaca ccgcgcgagc
     8221 gcatcgtaca ggcgatggag gagatcaaag aagaactggc cgccagacgc aaagtgctgt
     8281 tggaaaacaa caaactgctg gaagagcagc ggctgaccca gcgtacccag tttgatctgg
     8341 agatgatgga cgagctgggc tactgttcgg ggattgaaaa ctactcgcgc ttcctctccg
     8401 gtcgtggacc gggtgagcca ccgccgacgc tgtttgatta cctgcctgcc gatgggctgc
     8461 tggtcgtcga tgaatctcac gtcaccattc cacaaattgg cggcatgtat cgcggtgacc
     8521 gggcgcgtaa agagacactg gtggagtacg gcttccgcct gccatcagcg ctggataacc
     8581 gtccgcttaa gtttgaagag ttcgaagcat tagcgccgca aaccatctat gtttcggcga
     8641 cgccgggtaa ttacgagctg gaaaaatccg gcggcgatgt ggtggatcag gtggtgcgtc
     8701 caaccggatt gcttgacccg attatcgaag tgcggccggt ggcgacacag gttgatgatc
     8761 ttctttcgga gattcgtcag cgagcggcaa ttaacgaacg cgtgctggtc accacactga
     8821 ccaagcgaat ggcggaagat cttaccgaat atctcgaaga acacggtgag cgcgtgcgtt
     8881 atcttcactc agatatcgac accgtcgagc gtatggagat tatccgcgac ttgcgtctgg
     8941 gtgagttcga cgtattggta gggatcaact tactgcgtga aggtctggat atgccggaag
     9001 tgtcgctggt ggcgatcctc gacgctgaca aagaaggctt cctgcgttcc gaacgttcgt
     9061 tgatccagac cattggtcgt gcggcacgta acgttaacgg taaagcgatt ctctacggcg
     9121 ataggagcac tccatcaatg gcgaaagcga ttggcgaaac cgaacgtcgc cgtgagaaac
     9181 agcagaagta caacgaggaa cacggcatta cgccgcaagg cttgaacaag aaagtggtcg
     9241 atatcctggc gctggggcag aacattgcca aaaccaaagc gaagggcaga ggaaaatcgc
     9301 gcccgattgt tgagccggat aatgtgccga tggatatgtt gccgaaagcg ttgcagcaga
     9361 aaatccatga actggaaggg ctgatgatgc aacacgcgca gaatctggag ttcgaagaag
     9421 cggcacaaat tcgtgaccag ttgcatcagt tgcgtgagtt gtttattgcc gcatcgtaac
     9481 aggatagcga agaagactga tgacaaacgg aaaacagtct gatgcgctac gcttatcagg
     9541 cctacatttt ctccgcaata tattgaattt gcgcggtttg taggccggat aaggcgttca
     9601 cgccgcatcc ggcataaaca acgcgcactt tgtcttcaat aggaagccgg aattttcctt
     9661 cggatttccg ttaacctaaa gcctgtaacg ccttttccag cgcgttatgt aacaactggc
     9721 ggtcatgacg atacggaata tcgctggcct ccagtacctc ctggatcaca atccgctctt
     9781 tcaccgccga gacatccact tttggcccga cgatgaccgc atcaatgacc tttttaccaa
     9841 catactgctc cataattgcc agcttgcttt ccagcttcaa attagccgca ggtaaactca
     9901 actcacgccc cagattgccg atataaacca tcggcgctgg cgtgcggcgt aatgcctggg
     9961 cgatttcctt cagcagcaga attggcatca ggctggtata aaaactgcca ggcccaataa
    10021 tgatgagatc cgcttcattg atagcgtgaa ccgcctcacg cgttgcgggt acattaggcg
    10081 ttaacaataa ctcttgaatc ggcgtagtta actggtcgat attgacctcg ccgtaaactt
    10141 catgcccctg atcgtcaatc gccatcagat caacaggatg ctctgacatt ggaatcaaat
    10201 gcgtatccac tttcagcaga ttacgaatta aattgatggc ttccagaggc cgcacgctaa
    10261 ggtgatccag cgcctttaac atcaagtttc cgagattatg accggaaagt tcgccattgc
    10321 cgccaaaacg gtattcaaac atcgcggagg cgacgttcgg ttccgttatc agctggttga
    10381 ggcagttgcg catatcgccc caagcaatgc cgccttctga acggcgaata cgccccgtcg
    10441 agccaccatt atccgtggtg gtgacgatac ccgttaaacg agaacccaaa gacgaaagtg
    10501 atgagagaac gcgtcccagt ccatgccctc cgccgagagc aacgacacga tcaagatcag
    10561 ccagcgtacg attgcgcata tagtttcctg gagtcagatt atccgcgcta cagtagcgca
    10621 aattgcgggg aaacagcaat taacctgcca atattgagga tacaaactat tttcttctat
    10681 ctctaaagga cgatgcacgc tatgcctccc tgatgatgta tatcaaagta aaaccgccat
    10741 tttcccttat tctgtagcga aatagcacga tcatgacgct atatacatga ttgcatagcg
    10801 aaagtgtgga tggtaaaaat ctcatttaca cgctagtatc ggcttaacca ctaaacactc
    10861 tagcctctgc acctgggtca actgatacgg tgctttggcc gtgacaatgc tcgtaaagat
    10921 tgccaccagg gcgaaggaag aaatgacttc gcctcccgta tctggaaagg tgtacatggc
    10981 ttcacaactg actgatgtat ttgcgcgtaa gttttactac ttgcgcctgt cgattaccga
    11041 tgtgtgtaac tttcgttgca cctactgcct gccggatggc tacaaaccga gcggcgtcac
    11101 caataaaggc tttcttaccg tcgatgaaat tcgccgggtt acgcgcgcct tcgccagtct
    11161 gggcaccgaa aaagtgcgcc tgacaggagg agagccgtct ttacgccgcg actttaccga
    11221 tatcatcgcc gctgtgcggg aaaacgacgc tatccgccag attgcggtca ccaccaatgg
    11281 ttaccgtctg gaacgcgatg tggcgaactg gcgcgatgcg ggacttactg gcattaacgt
    11341 cagtgtcgac agtttggacg cccgccagtt tcacgctatt accgggcagg ataaattcaa
    11401 ccaggtcatg gcagggattg atgctgcatt tgaggccggt tttgagaagg tcaaagtcaa
    11461 taccgtgctg atgcgtgatg ttaatcatca ccagctcgac acctttctga actggatcca
    11521 gcatcgccct atccagctgc gtttcatcga actgatggaa acgggcgagg gcagcgagct
    11581 cttccgtaag catcacatct ctggtcaggt tctgcgtgac gagctactgc gtcgcggctg
    11641 gatccaccaa ttacgtcaac gcagcgacgg tcccgcgcaa gtcttttgcc atccagatta
    11701 cgccggagag attggcctta tcatgccgta tgaaaaagac ttctgcgcca cttgcaaccg
    11761 cctgcgcgtt tcctccattg gtaaactcca tctctgcctg tttggtgaag gcggcgttaa
    11821 cctgcgcgat ctgctggaag gcgataccca gcaacaggcg ctggaagcgc gtatttcagc
    11881 ggcgctgcgg gagaagaaac agacccattt cctgcatcaa aacaacaccg gtattacgca
    11941 aaacttatcg tacattggcg gctaaaacgt caaaaggaga gatcagatga gtcaggtaag
    12001 cactgaattt atcccgaccc gtattgctat tcttacggtt tctaatcgtc gcggtgaaga
    12061 agacgatacc tccggtcact atctgcgcga ttcggcgcaa gaagcgggcc atcacgttgt
    12121 cgatgaagcc attgtgaaag aaaaccgcta cgctattcgc gctcaggtat ctgcgtggat
    12181 cgccagcgac gatgtacaag tggtattgat tacgggtggt actggcctga cggaaggtga
    12241 tcaggctccc gaagcgctgc tgccgttgtt cgaccgtgag gttgaaggtt ttggtgaagt
    12301 gttccgtatg ttgtcgtttg aagagattgg cacttccacg ttgcaatctc gtgcggtagc
    12361 gggcgtcgct aacaaaacgc tgattttcgc catgccgggt tcgaccaaag cgtgccgtac
    12421 cgcatgggaa aatatcatcg cgccgcagct ggatgcccgt acgcgtccgt gtaatttcca
    12481 tccacatttg aagaaataag tatgtcgcaa ctgacccata tcaacgccgc tggcgaagcg
    12541 cacatggtgg atgtctccgc caaagcggaa accgtgcgtg aagcgcgggc ggaagccttt
    12601 gtcaccatgc gcagcgagac gctggcgatg attattgatg gtcgccacca caaaggcgac
    12661 gtatttgcca ctgcgcgtat tgccggtatt caggcggcaa aacgcacttg ggatctgatc
    12721 ccgctgtgtc acccgctgat gctcagcaaa gttgaagtca atttacaggc cgagccggag
    12781 cacaatcggg tgcgtataga aaccttatgc cgcctgaccg ggaaaaccgg tgtcgaaatg
    12841 gaagcgttaa ccgcggcctc cgtggcggcg ctgaccattt atgacatgtg caaagcggtg
    12901 caaaaagata tggtgattgg tccggtacgt ttgctggcga agagcggcgg caagtcgggt
    12961 gactttaagg tggaagcgga tgattaaagt tctttttttc gcccaggtgc gcgagttggt
    13021 gggaacagat gcaaccgaag tggctgcgga ttacccaacc gttgaagcgt tacgccagca
    13081 cctggctgcg cagagcgatc gctgggcgct ggcgctggaa gatggcaaat tgctggctgc
    13141 cgtcaaccag acgctggtga gttttgacca tccgctgact gacggcgacg aagtagcttt
    13201 cttcccgccg gtaaccggag gttaagatgg cagaaaccaa aattgttgtc ggcccgcagc
    13261 cgttcagcgt aggagaagag tacccgtggc tggcggagcg tgacgaagac ggtgcggtag
    13321 tcaccttcac cggaaaggtg cgcaaccata acctgggcga cagcgtcaaa gcattaaccc
    13381 tcgaacacta tccggggatg actgaaaaag cactggcaga aattgttgat gaagcgcgta
    13441 accgctggcc gctggggcgc gtcacggtga ttcaccgcat cggggaatta tggccgggcg
    13501 atgaaatcgt ttttgtcggt gtcaccagtg cgcatcgcag cagtgcgttt gaagccgggc
    13561 agtttattat ggattatctc aaaacccgcg caccgttctg gaagcgcgaa gccacgccgg
    13621 aaggcgaccg ctgggttgaa gcccgggaga gcgatctgca ggcggcaaaa cgctggtagt
    13681 tttttgttag ccggataagg caccgctgcg tccggcaatt tcaccgctca gtacatgcgt
    13741 ttacgctgcg tcgatgcaca gcctcatcat tttgcagtat ccttaagata ttccttatat
    13801 cttcaggaga tcgtcatgga cagattccca cgttctgatt caatcgtaca accctgggct
    13861 ggcttgcaaa cttatatggc gcaagtctat ggctggatga ccgttggctt gttgctgacc
    13921 gcatttgttg cctggtatgc ggctaattcc gcggccgtga tggagctgtt gttcactaac
    13981 cgtgtctttt taatcggtct gatcatcgcg caattagcat tggttattgt gttatcagcg
    14041 atgattcaaa agctgagcgc aggtgtaacg acgatgctct ttatgcttta ttcggcgctg
    14101 acgggtctta cgctttccag tatattcatt gtctataccg ctgcttctat cgccagtact
    14161 ttcgtcgtta ctgccgggat gttcggcgca atgagcctgt acggttacac cacgaagcgc
    14221 gatttaagtg gctttggcaa tatgctgttt atggcgttaa tcggcattgt gctggcatcg
    14281 ctggtcaact tctggctgaa aagcgaagca ttgatgtggg cagttaccta catcggcgtg
    14341 attgtctttg tcggattgac ggcgtatgac actcagaaat tgaaaaatat gggtgagcag
    14401 attgataccc gcgacacgtc gaacctgcgc aaatattcca ttcttggtgc gttaaccttg
    14461 tatctggact tcatcaacct gttcctgatg ttgttacgga tcttcggcaa ccgccgttaa
    14521 ttctttttca ccatcactat cccggccatg tgtcgggatt tttttcttac taaaaacgac
    14581 acttaacaag taccaggtat aaaatatgcc ttatgagcga gcggaatcgt tttccgtatc
    14641 cataaataca aaagtatttt gtatgcgtgg ttttaaattt attgatggtg aatattaatg
    14701 ttttttctaa ggacaactaa aagaatggaa tcatactcgc aaaacagcaa taaattagat
    14761 tttcagcacg aggccaggat attaaacggt atatggctca ttaccgcttt aggtttggtg
    14821 gcaaccgcag gactagcctg gggagctaag tatctcgaaa ttacggcaac caaatatgat
    14881 tcaccaccaa tgtatgtcgc catagggtta ttattacttt gtatgtatgg cttaagtaag
    14941 gatatcaaca agataaatgc cgccatcgcg ggcgtaatat atctgttttt actctctttg
    15001 gtggcgattg tcgttgcaag tttagttcct gtatatgcca ttatcatcgt gttcagcact
    15061 gcgggcgcga tgtttttaat cagtatgctg gccggtttat tatttaatgt tgatcctggt
    15121 tctcaccgtt ttatcattat gatgacgttg acagggttgg ccctggtaat catcgtgaat
    15181 gcggcattaa tgagtgaacg gcccatttgg gtaataagtt gcttaatgat tgtgttatgg
    15241 ccaggcatta tctcgcatgg acgaaataag ctccttgaac tggcaggaaa atgccatagt
    15301 gaagagttgt ggagtccggt tcgttgcgct tttacaggtg cattaacact ctattactat
    15361 tttatcggct tctttgggat acttgccgcg atagctataa cgcttgtctg gcaaaggcat
    15421 acgcgttttt ttcattagtc gtaaatgcag atactaaaga acttaactaa acttcacatc
    15481 gccgcttcat ttttcgcccg tagcttcttc gcttggcttt ccagtagcag gtaacagatc
    15541 agcgccagca gcagcgggat aaagtaatac aggacgcgat aagcgagcag ggcggcgata
    15601 atgctccctt tcgaggtatg ctccccagcc agtagtgcga taaacaccgc ttccagcaca
    15661 ccgatccccg ccggaatatg cacgatgacg ccagcaatac tactaaccag taacacgccc
    15721 agtacaaaga aatagttcac gctttgacca agtaacagcc agataatcgc ccccattacc
    15781 atccagttaa cgctggaaat cagcatctga gcgagggcga atttccatga aggcagcacc
    15841 agcttttgcc ctttgatggt catatggcgg tgcttcgcga aagcgcaaaa ccacaaataa
    15901 accgcgataa tcatcagtaa gccaatgccg agaatacgca gcgtagtttg atcgacatac
    15961 cagtaatccg gcaactccac cacgcctgcg gtaaagataa tccctgccag taaaatatag
    16021 cccagccagt tggtggtaat actgagcgag gaaatccgcg taatagtgct gcccggtaac
    16081 cctagtcggg agtacaaacg atagcgcata ccaatgccgc cgacccaggt actgagcgtc
    16141 aggttgaagg cgtagcagat aaacgacacc agcatcacct ggcgcttcgc cagtttgtga
    16201 ccgcagtaaa agcgggcgag caggtcatag cagccgtaaa tcagatagct gacgaccacc
    16261 agcccgaccg cactaagcag cgcaacgcga ttgtagtcgc ggatgacctt ccagacctct
    16321 tcccagtcca cttttttggc gtagaccacc agcaacacaa tcaccgcgat aaaaaacagc
    16381 caggtgagga tcttctttgc taagcgccag cgcgggtgtg atttactcat caggggttta
    16441 cccccgtgtt ttcagtttct acccgatcct gcgtttccat tgtcggttgt gcggggggat
    16501 caacctgcgt cagacgtggt gtgtgtgccg gaagccagcc aaccagcgcc gggaagtggc
    16561 gtaaaaagtg gaacgccagc acgcttttgg tcaggttcca ccaggtgcgt ttgggcagca
    16621 tggtttcatc cacctgctga caatctgcgg caataatgcc gttcagatta tcgcgcagcg
    16681 tctggttaaa atgacgatcg tggatgatga catttgcttc gagattcagt gacaaactga
    16741 gcggatcgag attactggac cctactgtcg cccagtgatc gtccatcaat gccactttgc
    16801 cgtggagcgg gcggcggcgg tactcaaaaa cctgaacgcc gcctttaacc agatagttat
    16861 acagcaagcg cgcaccgact ctgacaatcg gcatatccgg ttcgccctga atgatcagtt
    16921 tgatccgcac cccgcgccgt gccgctttac gcaaggcgtg taaaaatcga tagccgggga
    16981 agaagtaggc gttggcgata atcacttccc gctgcgcctg agtgagcatt ttcagataat
    17041 ggcgttcaat atcatcgcga tgttcttcgt tatcgcgcca gaccagcaat acctgcgctt
    17101 ctcccggctg gcggttctct tccgctttgt gatgacgtcg ccaccagcgt cgtgccgcgc
    17161 tctgtccagg caggttttcc agctcaaact ggagaatatc ttcgacaatc ggcccttcaa
    17221 ggcgtaccgc gtaatcctgt ttagcctctg gaccgtagct ggacatatgc tcggcggagt
    17281 aattcagccc gccaataaag gcgatacgcg cgtcgatcac cacaattttg cgatgcatcc
    17341 ggcgaaacac attggtgcgc ataccaaaaa ggcgagggcg gggatcgtag tagcggaaca
    17401 ctacgccagc tgccgtcagt tcattgacaa actcatcgct gagatccggc gaaccgtagc
    17461 catccagcaa gacttccgct ttaaccccgc gttgcgctgc tgccagtagt gccgcatgca
    17521 gctgtttgcc gacgttatcc tcaaaccaga taaacgtttc aagaatgatg cgttcttgtg
    17581 cctcgccaat cgccttaaac accgcgggat aatattgctc gccgttttcc agcaactgga
    17641 tcttattgcc ttcgcgccag ctacatttca taaatgaatc tccgcactta aaggggcatg
    17701 atcagaaagg tgtcgccatg tccgcagcgg caacgcggtt ggcgcgctgg cgctggcatt
    17761 tttgacgtag atcctgtcca gtcgtagtag aggaaattgc accggaaacg tgcgcgccgg
    17821 gcgtccgtgg gcgcgggtaa aaatctcatc cagtccggcc tgcactttta acggatgatt
    17881 agctttttgc cgccagtcat tgaaatcacc cgccaccaat accggttcgc cgtccggtag
    17941 ctcattcacc cattcggcga gcatcgcaag ctgcgcctga cggtgcgcct cacgcaggcc
    18001 cagatgtacg cacatcacat gaatcgcttt tccggtcatc ggcggcacaa tgcggcagta
    18061 gagcacgccg cgcttttccg caccatcgac cgaaacatcg cgattctcat aatgttcaat
    18121 gggataacgc gacagtacgg cgttgccgtg atgcccttcc gggtatacgg cattgcgacc
    18181 gtaggcaaaa tcgctccaca tagtgtcggc gagaaactcg tagtgcgagg tatcgggcca
    18241 gttttccaca tgcagcggat gaacttcgtg cgcgcccatc acttcctgca ggcaaacaat
    18301 atcggcgctg acggtacgca cggcatcgcg aagttccggc aaaatgaagc gtcggttaaa
    18361 cgcggtaaag cctttgtgaa tgttgatggt gagcaccttg aacgaaaatt gttgtgtttg
    18421 atcgggcata attttcctgt ctttgcctct tatctcattg aaatagtgta gtcggcgtca
    18481 caaaaaggtg cggtcttacg gaattttccg taaagttcgg tactctgagt aagtagagat
    18541 aaattcttca ggagagaagc catgaagtgg caacaacgtg ttcgtgtcgc aacgggtcta
    18601 agttgctggc agattatgtt gcatttactg gtagtggcgc tgctggtggt gggctggatg
    18661 agtaagactc tggttcacgt cggcgtggga ttatgcgcac tgtattgtgt cacggtagtg
    18721 atgatgctgg tgtttcagcg ccaccccgag caacgctggc gtgaggtggc agacgtgctg
    18781 gaagagctga ccacgacctg gtattttggc gcagcgctga ttgtgctgtg gctgttgtcc
    18841 cgcgtactgg aaaacaactt tttgctggca attgcagggc tggcgatcct tgctggcccg
    18901 gcggtagtgt ctttgctggc gaaagataag aagttacatc accttacgtc taaacatcgc
    18961 gtacgccgct gaccctgtcg tggccgttat caccagtagc ggccacaaac tattccacac
    19021 aatatccaga ctcgcatcct tcaaataaat ctgcttggta atgtccgtaa agtggcgaat
    19081 agggttaatc cacgtcagat tttgcagcca cactggcatg ttttccaccg gcgaaacgta
    19141 accggaaagg agaatggcgg gcatcataaa aacaaacacg ccgataaacg cctgctgttg
    19201 tgtcgagcag agtgatgaaa tcaacagacc gaatcccacc agcgataaac cgtaaatcac
    19261 catcgtaaag tagaacagcg ctagcgatcc ggcgaagggg atttgatacg cccagatacc
    19321 aatcgccagc acaatggtgg cctggaaggt ggcgacaatt aacgccggta cggctttgcc
    19381 gatgaagatc tgccaggtgg tgagcggcga aaccagtagc tgatcgagcg taccttgttc
    19441 acgttcgcgg gcgacggaaa gtgaagtgac gatcattacg ccgatagtgg tgatcatggc
    19501 gatcagcgac ggcaccacaa accatttgta gtcgagattc gggttatacc agttgcgtac
    19561 caccagctcg ctgttgttgg gtttcggttt tccttccagc agctcctgct gataattttt
    19621 gacgatctgt tgcaggtagt tggcggcaat ttgcgcactg ttggagttac gcccgtcgag
    19681 gatcaactgc aacggcgcgg tctggaaggt atccagtttg cgcgagaagt cagccgggaa
    19741 acgcaccagt agtaacgcct tttgtgtgtc gatggtcggg cggatctcct gtgggctttt
    19801 cagcagcagc acatgtgtaa aggcgctggc gcgggcaaaa cgttgagtaa gttccaccga
    19861 atgctcgccg ttatcttcat cgtagatggc gatggtggcg ttagtcactt ccagcgtggc
    19921 ggcgaacggg aacaggatca cctgaattag cacgggtaaa atcagaatcg cgcgggtttg
    19981 tggttcgcgc agcaacgact gcaactcttt gcggattaac gtccataagc gatgaaacat
    20041 gctcttctcc ctaatccagc cgacgtttgg ttttcagcca cgtcaggccg ataaacatca
    20101 ccgccgaagc gatcaaaaac agcacgttta ccaccagcac cactggaata ttcccggcga
    20161 ggaacaggct ttgcagggtg ctgacgaaat aacgagcggg aataatgtac gtcaccgcgc
    20221 ggatcaccgc gggcatactg tcgatctgaa aaataaagcc ggaaagcata atcgacggca
    20281 gaaaagcggc gttcagggcg acctgagcgg cattgaactg gttgcgggta atcgtggaaa
    20341 tcagcagccc cattcccagg gtactgagta aaaacaggct ggagataaaa aacagaatca
    20401 gcagcgaacc gcgatacggc acgccgagaa taaacactga caccagcata cacagcaaca
    20461 tcgccagcat cccgagaaag taataaggga tcagcttaca cagcagcagt tccgtgcggg
    20521 taatctccgt agagagcaga gcctccatgg tgccgcgttc ccattctcgc gccaccacca
    20581 gcgaggtgag aatcgcgccg atgaccgtca tgataatggt caccgcaccc gggataataa
    20641 agtgctggct tatcgccgcc gggttaaacc agtagcgggt ttgtacatca ataagcggtt
    20701 caaaagtctg cccgttgtcc tccgctcgct gcatttgcca gatctgccag atcccttcga
    20761 catacccctg cacaaagtta gcggtattcg gctcactgcc gtcggtgatc acctgaatcg
    20821 gtgcggtggc gttggcgcgc tccatctgtt cggcgaaatc cacgggaata accaccagac
    20881 cgcgaatttt ccccgactgc attttggcga tcagttcctg acggttatcg ctgatggtgg
    20941 cgtcgatgta gggcgaaccg gtcatggtgt gggtgaaatc cagcgccgct tcgctacgct
    21001 gttccagcaa aatcccgacc cgcagcttgc tggagtcgag gttaatgccg taaccaaaaa
    21061 taaacagcag tagcagtggg atcactaccg caatcagcca gctactcgga tcgcgaacga
    21121 tctgccgcgt ctctttgacg cacagcgccc gcacgcgatg ccaggaaagg atcgggttac
    21181 tcattgctat gctccttatc ccagtcgtgg atcaactgaa taaaggcttg ctccatggtg
    21241 ggatcgggtt gctcatcgtt agctgactgt gctttcaaat cgtccggcgt accgctggcg
    21301 attaatttcc cacggtacac caggccgatg cggtcgcaat attccgcttc atccataaag
    21361 tgggtggtga ccatcaccgt gacgcctttc tctaccatgc tgttgatgtg cagccaaaat
    21421 tcacggcggg tgagggggtc aacgccggaa gtcggttcgt cgagaaagag aatatccggt
    21481 tcatgcatca gcgaacaggc cagcgccagc cgctgtttaa aacctaatgg cagttcatcg
    21541 gtggcgtgtg aggcaatact tttcaggcca aatgcctcgc tcatacggga gattttttcg
    21601 ttctgcgccc gaccgcgtaa gccatacaca ccagagaaaa agcgtaaatt ctgttcgacc
    21661 gtcaggttgc cgtagagcga aaatttttgc gccatatagc cgagatgctg gcgcgcttta
    21721 ccggaactct ctttcagatc catccccagc accagcgcct ggccggaagt cggcaccagc
    21781 aaaccgcaca tcatcttaaa ggtggtcgat ttacccgcgc cgtttggccc cagcaaacca
    21841 aaaatctccc cacgtttaac ggcaaagttg acgtgatcgg tggcggcaaa atcaccaaat
    21901 ttcttggtca gttctttcgc ttcgatcacc gtctcgccgg gtgtgccttc taccgtatgc
    21961 aatattgcac ccagcggcga ttctgaggtt ccggcaccgc ccagcaaatc aataaacgca
    22021 tcttcaaaac gcggcgtagt ttcgttgatg ttgatttccg gcatcccgtc ggcatggcga
    22081 atatcgtctg gtgtggcctc ttttttgagg atcagacgta ccgatttccc ctgaatcatg
    22141 ccatcgctga cctgcggcag tttcaaggcg cgttgcaaca gtttgcggtt gccctcgtgt
    22201 ggactggtca tcagaaagct gcgtccggcc atggtttgtg tcagggcttt tggttctccc
    22261 tgatacagca actcgccttc gttcatcagt aacacgtcac ggcactgctc ggcttcgtcg
    22321 agatacgagg tactccagag gattaacatc ccttcgcccg ccagctcatg caccatctgc
    22381 cacagttcgc gccgtgagat agggtcaacg ccgacgccgg gttcatcgag cagcaacact
    22441 ttcggttcgc ccaccagggt acaggccagg ccgagttttt gtttcatccc accggagagc
    22501 ttgcccgcca ggcgtccggt aaacggccca agagacgtaa actccagcag gcgagcaaaa
    22561 gtttgcttac gtgcctcgcc ggtgacgctg cgcaaatccg cgtacagatt gaggttctcc
    22621 atcaccgtga gatcttcata cagaccaaat ttctgcggca tatagccgag cacggcgtgc
    22681 agcgcgccgt cgtttttgat cggatcaaag ccaatcaccg tggcactgcc gctgtcgggt
    22741 ttcagtaatc ccgccaacat ccgcatcagt gtggttttac ctgcaccgtc cggccccacc
    22801 aaccccgtca cataaccggc gtgaatggta caatcgagcg gcgcgacggc gggcttgtcc
    22861 atgcccggaa agcgtttttc caggccgttc agcgtgataa cggcatcatt catgtcctgc
    22921 ctcgtcaccg aattgtaccg tcactggcat tccctggcgt aacgcatcat cggcgtcggt
    22981 caccacaata cgcaggcgat agacgaggtc ggtacgcaga tccggtgttt cgacggtttt
    23041 cggggtaaat tcagcagtcg gcgaaacgaa accaatctgc ccgtgatacg gcttgtccgg
    23101 gcgaccatcg gtataaagca gcactttgcg ccccggctgg gcctggtcaa gattccgttc
    23161 atcaacataa gcgcgcaccc acaccggacg cgttagtgaa acggtaaaca ccgtgccacc
    23221 ttcattgaga accgtgcctg gctctaccgc gcgcgttaac agcgtgccat cagacggtgc
    23281 tatcaacgtt gagtcctgta aattcagctc cgcctgcgcc agttgcgcct gcgcctgttc
    23341 gaggctggct ttcgcctgag cgatgtcctg ttcacggtta ccggaacggt actgacgcaa
    23401 tttatcctgt gctgatttca gcgttgcctg tgcctggtcg cgcgaggagc gggcattttc
    23461 caggtcattt gccgaaatgg tgcggctttt ccacagtcct tgctggcggt tatagaagtt
    23521 ctgcgcatag tcataggcgg cttgcgcctg tttcaccgct gcggcggcct gagcgatttc
    23581 ttcatcgcga tacccggcaa gcatcagatc atactgcgcc tgtgccaccg agacgcccgc
    23641 tttcgcctgc atcagggcaa tctcatacgg cttgtgatcc agttcgccca gcacctggcc
    23701 cgctttgata gcatcacctt cgtccaccgc cagcgattca acgcgccccc caacacggaa
    23761 actaagattc accgtacgaa tatccacgtt gccatacagc gtcaggccgt tatcctggcg
    23821 gctttgatac caccagtagc ctccggcaac cacggcggca agtaccacta ccgccagtcc
    23881 gatcacgaca ggttttttca tcactacaaa ctcctttgcg ataatccttg caaaatcagg
    23941 tcgatatgac aggtcaccgt ctggttgatc agttcggtct tttcttcatc gaacgcggtc
    24001 cagccggtac gtaacagaat tgtttctttg ccaagacgga acgccagaat ctcaccaatc
    24061 aacgcatggg tatgaaggat catgcgggtg tcattagcgt cgcagccggt ccaggcggca
    24121 atcaggcgtg tcaggtggct gtgtagcgga ctaatcacct gttcgtgcac caggtggtag
    24181 gctgccgtgg gagagagctg ctcacgggag ataaacttgc tgaggttgac ggtgtcatcc
    24241 tgggtgagca gcttaatcat gttcctgcag gcgcgaagga tcagttcacg gatggcagcc
    24301 cgatcaggct gtggttgggc gaacaagcgt tcggcttcct cggcatgcgg acggaactgc
    24361 tcgccaataa aatcggcaat ccactgggcg caggcgaggt acaaatcttc tttcgaaccg
    24421 aagtagtagg tgatggcagc gatattctgc ccggcctggg cggctatctc gcgggtggtg
    24481 gcgttcattc catattcacc aaactgcgcc agtgcggcag caatcagctg ttttttcgcc
    24541 tgttcaccct tgattgtcat ggcaggatta ttcatcgcac agtccattct taatcaaatg
    24601 attgattaag attatgactc catatgggag ttgtccagta tggctaagaa ttttagctac
    24661 gccagtcaca gggataattt atgcgctgcg tcacaaaaac tgctacactc cgctccctca
    24721 tgacattgtg gtttttgtca ttttcctttt cagtatctcc ctgaaaacta caccggtaac
    24781 ggtcggggcg gttcggagta gttatgtctt tcgattcttt gggtttaagc cctgatatcc
    24841 tgcgcgccgt tgccgagcag ggttaccgtg aacccacccc tattcagcag caggcgatcc
    24901 ctgctgtgct ggaaggccgc gacctgatgg ctagcgccca gaccggcacc ggcaaaacag
    24961 cgggctttac gctgccgctg ttgcaacacc tgatcactcg ccagccgcac gccaaagggc
    25021 gtcgtccggt acgtgcgctc attcttaccc cgacccgtga actggcggca cagattggcg
    25081 aaaacgtccg tgattacagc aaatacctga acattcgttc gctggtggtg tttggcggtg
    25141 tcagcattaa cccacaaatg atgaagttgc gcggcggcgt tgatgtgctg gtggcaacgc
    25201 cgggacgttt gctggacctg gaacatcaaa atgcagtgaa gctggatcag gttgaaatcc
    25261 tcgtcctcga tgaagctgac cgcatgctcg acatgggctt tatccacgat atccgtcgcg
    25321 tgttaacaaa actgccagcg aaacgccaga acctgctgtt ctctgcgacc ttctctgacg
    25381 atattaaagc cctggcggaa aaactgttgc acaacccgct ggaaatcgaa gtggcacgcc
    25441 gcaataccgc gtctgatcag gtgacccagc acgttcactt tgtcgataag aaacgcaaac
    25501 gcgaattgct gtcgcacatg attggtaaag ggaactggca gcaggtgctg gtgtttaccc
    25561 gtaccaaaca cggcgctaac catctggctg agcagctcaa taaagatggc atccgtagtg
    25621 tggcgatcca cgggaataaa tcgcaaggtg cgcgtacccg tgcgctggct gattttaaat
    25681 cgggtgacat tcgcgtactg gtggcgaccg atatcgctgc gcgcggtctg gatatcgaag
    25741 agttgccgca cgtcgtcaac tatgaactgc caaacgtacc ggaagattat gtccaccgta
    25801 tcgggcgtac cggtcgtgcg gctgctaccg gtgaagcgtt gtcgctggtg tgtgttgatg
    25861 aacacaaact gctgcgtgat atcgaaaaac tgctgaaaaa agagatcccg cgcattgcga
    25921 ttccgggcta tgagccggac ccgtcaatca aagccgaacc gatccagaac ggtcgccagc
    25981 aacgtggcgg catcaggctg atgaacaaac gcaaaactgc ctgatgcgct acgcttatca
    26041 ggcctacgtg aactctgcaa tatattgaat ttgcatgctt ttgtaggccg gataaggcgt
    26101 ttacgccgca tccggcattt cacaacaagc acttgtcagc aatttgagaa cacgggaaaa
    26161 aatttatctg ttttaccgcc cataagccac cgagacgtta cttctctata gccaattgtt
    26221 cgcgcaactc cattaaaagg tagcccagtc gattcttgcc cttaccatta ccaccgtctc
    26281 cccagtaagc atcgttttcc gtatgctcaa ccagttttgc gggcgcggtt gccagcaaga
    26341 gcacacgcag ttctgcatgc tgttcgaatt tagcgcgaag tgctttacgc atcacctgtt
    26401 ctttaaccga ctcccagttt ttacgaagtg gcttggaacg attacgcccc atgcgagcgg
    26461 cgaccatggg ggaagaaacc cgacggatct cttcgcggta tttttcatca aggaattttt
    26521 gtgcctgaaa atagtgttcg gaggtgggcc aggtattccc gtcaaccttg atgggcctgg
    26581 cggcaaagtt agaaaaatca ccgtagtcat cgctggtgct gtagaaattt atgatggtgt
    26641 cttgcatgac gtgttggatc ctttgtgctc gaacgggcat taaaccgcat tatgttggtg
    26701 gttattgcga gccgctttcc agaaacagaa aaaccattac ccctgaaaac cgaaaaatgc
    26761 cacaatattg gctgtttata cagtatttca ggttttctca tggcattaac cgccgcgctt
    26821 aaagcgcaaa ttgccgcctg gtataaggcg cttcaggaac agatccccga ctttattccc
    26881 cgtgcgccgc agcggcagat gattgcggac gtcgccaaaa cgctggccgg agaagaaggg
    26941 cggcatctgg cgattgaagc ccccaccggc gttgggaaaa cgctctccta tttgatcccc
    27001 ggcatcgcca ttgcccgcga agagcaaaaa acgctggtgg tgagtaccgc caacgtggcg
    27061 ttgcaggatc agatttacag caaagattta ccgctgctga aaaagatcat tcccgatctt
    27121 aaattcactg ccgctttcgg gcgtgggcgc tacgtttgtc cacgtaatct gacggcgctc
    27181 gccagcactg aacccacgca acaggatctg ctggcgtttc ttgacgacga actgacgcca
    27241 aataatcagg aagagcaaaa acgttgtgcg aagctgaagg gcgatctcga cacttataaa
    27301 tgggatggtc tgcgcgatca tacggatatc gctatagatg atgatctctg gcgtcgtttg
    27361 agtaccgaca aagccagctg cctcaaccgt aactgttact actatcgtga atgcccgttt
    27421 tttgttactc gtcgggagat tcaggaagcg gaagtggtgg tggcaaacca tgcgctggtg
    27481 atggcggcga tggaaagcga agccgtactg cctgacccga aaaatctgct gctggtgctg
    27541 gacgaaggtc atcacctgcc agatgtggcc cgggatgcgc tggagatgag cgctgaaatc
    27601 accgtgccct ggtatcgact acagctggac ttgttcacga aactggtcgc tacctgcatg
    27661 gagcagtttc gcccgaagac catcccaccg ctggcgatcc ctgaacgttt gaatgcgcat
    27721 tgtgaagagt tgtatgagct tatcgcctcg ttaaacaaca ttctcaatct ctacatgcct
    27781 gccgggcagg aggcagagca ccgttttgcg atgggcgaac tgcctgatga attgctggag
    27841 atctgccagc ggctggcaaa actcaccgag atgctgcgtg gtctggcgga gttatttctt
    27901 aacgatttaa gtgagaaaac cggcagccac gatattgtgc gtctgcatcg gttgatttta
    27961 cagatgaacc gcgcgctggg gatgtttgag gtgcaaagca aactctggcg gctggcgtct
    28021 ctggcgcaat cttccggtgc gccggtgacc aaatgggcga cgcgggaaga gcgcgaaggg
    28081 cagctacacc tctggtttca ctgcgtggga atacgtgtca gcgatcagct ggaaaggctg
    28141 ctgtggcgca gtattccgca cattattgtc acctccgcaa ccttgcgttc gctgaatagt
    28201 ttttcgcgtt tgcaggagat gagtggtctg aaagagaaag cgggcgaccg ttttgtggcg
    28261 ctggattccc cctttaacca ctgcgaacag ggcaaaattg ttattccccg gatgcgcttt
    28321 gagccttcca tcgacaacga agagcaacac attgccgaaa tggcggcctt tttccgtgaa
    28381 caggtggaga gcaaaaaata tctcggtatg ttggtactgt ttgccagcgg acgggcgatg
    28441 cagcgctttc tcgactatgt gacggattta cgcctgatgt tgctggtgca gggcgatcag
    28501 ccgcgttatc gcttagttga actgcaccgc aaacgcgttg ccaacggtga gcgtagcgtg
    28561 ctggtgggct tacagtcatt tgccgaaggg cttgatttga aaggtgatct gctcagccag
    28621 gtgcatatcc acaaaatcgc ttttccgccc atcgacagcc cggtggtgat caccgaaggg
    28681 gaatggctga aaagcctcaa ccgctatccg tttgaggtgc aaagcctgcc gagcgcctcg
    28741 tttaacctga ttcagcaggt tgggcgactg attcgaagcc acggttgctg gggcgaagtg
    28801 gttatctacg acaaacgctt gctgacaaaa aattatggca agcgactact ggatgcatta
    28861 ccggtatttc cgatagagca accggaagtc cctgaaggta tagttaaaaa gaaagaaaaa
    28921 acgaaatccc cacgccgtcg gcggcgttaa tgatgtgagt caggtaagga gtcgtaaatg
    28981 gactatcgca aaatcattaa agagatcggg cgcgggaaaa accacgcgcg cgatttagac
    29041 cgggatactg cgcgcggtct gtatgctcat atgctcaatg gtgaagtccc tgaccttgag
    29101 ttgggcggcg tattgattgc gctgcgtatc aaaggggaag gggaagcaga gatgctcggc
    29161 ttttacgaag ccatgcaaaa tcacaccatc aagctgacgc cgcctgcagg caagccgatg
    29221 ccgattgtca tccccagtta caacggcgcg cgtaaacagg ctaacctgac gccattattg
    29281 gcgattctgc tgcataaact cggttttcct gtggtggttc acggagttag cgaagatcca
    29341 actcgcgtgc tgacagaaac cattttcgaa ctgatgggta ttacaccaac gttgcacggc
    29401 gggcaggcgc aggcgaagct cgacgaacat caaccggtgt ttatgcctgt cggcgcgttt
    29461 tgcccgccgc tggaaaaaca actggcgatg cgctggcgga tgggcgtgcg taacagtgca
    29521 cataccctgg cgaaactggc gacgccattt gccgaaggtg aggcactgtg tctttccagc
    29581 gtttcgcatc cggaatacat tggacgcgtc gcgaagttct ttagcgatat cggcgggtgg
    29641 gcgttactaa tgcatggtac tgaaggcgaa gtgtatgcta atccgcagcg ctgcccgcag
    29701 atcaatctca ttgaccgtga agggatgcgg gtgctgtatg aaaaacagga ccctgctggt
    29761 agcgagttac tgccacaagc aaaagatccg gaaaccacgg cgcagtggac tgagcgttgc
    29821 ctggctggca gcgaagcgat tcccgaatcg ctgaaaatcc agatggcttg ctgcctggtg
    29881 gctacgggtg aagcggcaac tatcagcgat ggcctggcgc gcgttaatca ggcattttaa
    29941 tttttatctc agccggatga gccacgtcat ccggctttta ccccaccgca atatgaaatt
    30001 cctgcatctt tattgacctt cccgcgcccg gcgtgcagca taaaaaaaca acaaacacat
    30061 aacataaaca ggagttaacc atggaaagtg gtcatcgctt tgatgctcag acgctgcaca
    30121 gttttattca ggctgtattt cgtcagatgg gtagcgagga acaagaagcg aaattagttg
    30181 ccgatcattt aatcgcggca aacctggcag ggcatgattc tcatggtatt ggcatgatcc
    30241 caagctatgt gcgttcctgg agtcaggggc atctgcaaat taaccatcat gccaaaatcg
    30301 ttaaagaggc gggggcggca gtcacgctcg atggcgatcg cgcattcggt caggtcgcgg
    30361 cacacgaagc gatggcgctg gggattgaga aagcgcatca gcacggtatt gctgccgtgg
    30421 cgctacataa ctcgcatcat atcggccgta tcggttactg ggcggagcag tgttcagcgg
    30481 cggggtttgt ctctatccac tttgttagcg tggtcggtat tccaatggtc gcgccgttcc
    30541 acggtcgcga cagccgcttt ggcaccaatc ccttctgtgt ggttttccct cgtaaagata
    30601 atttcccgct gttgcttgat tacgccacca gcgccattgc atttggcaaa acccgcgtcg
    30661 cctggcataa aggcgtcccc gtgccgccag gttgcctgat tgacgttaac ggcgtgccga
    30721 cgaccaatcc ggcggtaatg caggagtcgc cgttggggtc gctgttgacc tttgccgaac
    30781 ataaaggcta cgcccttgca gcgatgtgtg aaattcttgg cggggcgctt tctggcggta
    30841 aaacgacgca tcaggaaacg ttgcaaacca gccccgatgc cattcttaac tgcatgacca
    30901 ctatcatcat caacccggaa ctgttcggcg cgccggattg taacgcgcag accgaagcct
    30961 ttgccgagtg ggtgaaagcc tcgccgcatg atgacgataa gccgattttg ctaccgggcg
    31021 agtgggaagt gaacacgcgt cgcgaacggc aggagcaggg gattccattg gatgcgggaa
    31081 gctggcaggc catttgtgat gcggcgcggc agattggtat gtcggaagag acgttgcagg
    31141 ctttctgtca gcagttagcc agctaaaaaa aagcccgtcc agtggcggac gggcaaacaa
    31201 gggtaacata ggatcaatga gggttagagc atatgcgtct gtcggcaaac agacagggaa
    31261 atacttgtgc tggacgtagc gtaaacgcct gatccgtcca acggttcgga ttttgtaggc
    31321 ctgataagac gcggtagcgt cgcatcaggc atctgttgcc ggatgcggca taaacgcctt
    31381 atccgtccta cgaattactt ataaattacc gcagtaccgc ttaatttatt gttgttggtg
    31441 gcggaagtga tgctgtatcc actggcaccg gctgccgccg ctttctcagc cagtttcgct
    31501 tccagggcat cgagggggga tgcgccatcg gcagaaacca cgccgatttt attcatgttc
    31561 tgtgcctggg atgccgttac cggttccgcc gcgaacacgc caaatgacag agttgaaaga
    31621 gccatagcag caacaacagt attgatagtt ttcataatta atctctcgca ggtgattttt
    31681 gtataagtga cgttgtttcg tcgatgtgat gagtatcacg tttttttacg agagataaaa
    31741 tcgaagagaa ttgaccgcct tgttcaaata aattgattga tatctaacat actgataaac
    31801 catttatttg ttgtacttat ggacttgttt tatgccgcgt ggcagtcaca gcagcgttaa
    31861 aggcacggaa tgccacgcaa aatacaaatt atactcgcta tgcatctcgc cagttgtagt
    31921 taacgtaact gtctgtcttt cgaacctctg cgattatatc ctgtatacgc tggtttttgt
    31981 aaatctttct cctgctgaca atgaatacat aagcgcacgc caggaatggc ttcccgacgg
    32041 gcttgcggga tgggggcacc gcactcttca cattcataca ggctttcgcc acgcggaatt
    32101 tcaccccgag cgcgggcaat cgcatcttca attgtactgt tgatctgttc gttgacggcg
    32161 tcatcgttag cccaagcgga tgccatatcg tcctccccat atcaatgctt gtacagttaa
    32221 gtgtagctaa tatagggacg aacctgggca gttcaagcgt cagatctccg accattcccg
    32281 cagcagatta tgataaagat tgagcagcga caggatctct tcattttcac catagcggct
    32341 tttcagcgac tgaatatttt tgtccagttc aaacagcatg gcgcgctttt tatcatcgcg
    32401 gatcatcgac tggatccaca taaatgatgc cactcgtacg ccgcgggtta cgggtgtcac
    32461 gcaatgcagg ctgctggagg gatacaacac cagatcacct gccgggagtt ttacccgatg
    32521 ttgtccaaag gtgtcattaa cgaccagttc gccgccgtcg tagctttgtg gatcgcttaa
    32581 aaacagcgtg gcagaaaggt cagtgcgcat ccagccgttt tgtggatggc tgcgtactgc
    32641 gccatccaca tggaaaccat aggtttcatt gttctgatag cgattaaaca gcggcgtgga
    32701 aagggtacgc ggcaaggcag cggcaaagaa caatgcatgt tggttaaccg cgttcagcac
    32761 ctcattttgc agtgcggcgt ataacgcgct gcgggtgtct acttgctgat tgttcttaac
    32821 ttgtgcacct tgtgctccgg tggtgacgcg tccatccacc cattcggctt gttccagttg
    32881 ttcgcgaaaa tgggcgacgt cctgtggcga taacacgccg ggaatgtggt acatcataca
    32941 agttctccaa aaagtggggc ctgcgcccca catctgaatc agaaatgcat attggctgtg
    33001 agcaagaagg ttcttggctc gcccgggtga taacggtagc cgctcttgtt gattgaggcg
    33061 acgtaatcgg tatcaaacag gttgtaaacg tttagctgga agtcgagatt gcgattaacc
    33121 cgatacccca gtttggcatc ggcgacccag taaccttctg taaatgctgg cgttcccacc
    33181 gcgccgtctg aacctttatg tatactgccg atatagcgtg cgcccgcgcc aacagagata
    33241 tcatcggttg cctgatattg gctccataag gtgaaggcgt gctccggggt atacggcagc
    33301 gatgaggaac catcctgggc aacatctttg ccgtttttga tggttgcttt ttgctgggta
    33361 tagccgccaa tcacctgcca cgcgggagtg atattccctg ccacggatat ctcatagcct
    33421 tcgacgcgtt tcttaccgta ttgcgagtaa gtcccgtcat cattttgctc aacttcattt
    33481 tcgatatcag tacggaacag cgcggcggtg agcaacaggc gtttatccag aacctgccat
    33541 ttggtgccaa tctcgctggt gttggctttt tgcggtttaa aatcggtgcg gttggcactg
    33601 ttaccactgc cagactgcgc aagggcgaag ttgttgccgc ccggaggctg ctgggaaacg
    33661 gcatagttaa tatagacatt gccgttttcc gtcaggtggt acagcgcccc ggctttccag
    33721 ttcaccagat tgcccgactt ggcggtgtcg acggtggtga ctggagaacc ttttgccaca
    33781 ccagctgggc aggtgatggc accgcgtccg ctgccgccgc aggcggtggc actgtcatat
    33841 tcagtatgat aattatccag acggatcccg ccgttcagct caaaatcacg ggtgatttgc
    33901 agcgtatcga aggcgtaaat tgcgaaggta tccgtctgac cattggcgtt tgcgccgttg
    33961 cgcgtcaggc cgccgggatg aattctgctg tcaggatgat aaatatttac cgccggtaac
    34021 gtcaccggat taacgccata gttagtctgc gtttcacggg taaattccac gccggtactg
    34081 acatcatgac cgatagaacc ggtatagaac gtcgaggtca ggttggtctg gttggtgaga
    34141 attttattac tcacatcttt ggtattcgcc gtgcgtgact aggtccagct attcacatcg
    34201 ctggtgggct gagtaatatt cgacgccccg cccataatcg ccgtcatcag gtaatcctgc
    34261 tttacgcgcg accaacgggt agtattgcga a
//
LOCUS       DAJTYM010000035        34007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTYM010000035 DAJTYM010000000
VERSION     DAJTYM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            512..2797
                     /gene="nrdA"
                     /locus_tag="OO575_001722"
     CDS             512..2797
                     /gene="nrdA"
                     /locus_tag="OO575_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6664717.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3031..4161
                     /gene="nrdB"
                     /locus_tag="OO575_001723"
     CDS             3031..4161
                     /gene="nrdB"
                     /locus_tag="OO575_001723"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6664718.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4161..4415
                     /gene="yfaE"
                     /locus_tag="OO575_001724"
     CDS             4161..4415
                     /gene="yfaE"
                     /locus_tag="OO575_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6664719.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4469..5119)
                     /gene="inaA"
                     /locus_tag="OO575_001725"
     CDS             complement(4469..5119)
                     /gene="inaA"
                     /locus_tag="OO575_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6664720.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5298..5540
                     /locus_tag="OO575_001726"
                     /pseudo
     CDS             5298..5540
                     /locus_tag="OO575_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5582..6658)
                     /gene="glpQ"
                     /locus_tag="OO575_001727"
     CDS             complement(5582..6658)
                     /gene="glpQ"
                     /locus_tag="OO575_001727"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6664721.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6663..8021)
                     /gene="glpT"
                     /locus_tag="OO575_001728"
     CDS             complement(6663..8021)
                     /gene="glpT"
                     /locus_tag="OO575_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6664722.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8294..9922
                     /gene="glpA"
                     /locus_tag="OO575_001729"
     CDS             8294..9922
                     /gene="glpA"
                     /locus_tag="OO575_001729"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6664723.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9912..11171
                     /gene="glpB"
                     /locus_tag="OO575_001730"
     CDS             9912..11171
                     /gene="glpB"
                     /locus_tag="OO575_001730"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6664724.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11168..12358
                     /gene="glpC"
                     /locus_tag="OO575_001731"
     CDS             11168..12358
                     /gene="glpC"
                     /locus_tag="OO575_001731"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6664725.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12551..13501
                     /locus_tag="OO575_001732"
     CDS             12551..13501
                     /locus_tag="OO575_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6664726.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13542..14012)
                     /locus_tag="OO575_001733"
     CDS             complement(13542..14012)
                     /locus_tag="OO575_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6664727.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14067..15269)
                     /locus_tag="OO575_001734"
     CDS             complement(14067..15269)
                     /locus_tag="OO575_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6664728.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15356..15898)
                     /locus_tag="OO575_001735"
     CDS             complement(15356..15898)
                     /locus_tag="OO575_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664729.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16177..16602
                     /gene="nudI"
                     /locus_tag="OO575_001736"
     CDS             16177..16602
                     /gene="nudI"
                     /locus_tag="OO575_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6664730.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16641..17243)
                     /locus_tag="OO575_001737"
     CDS             complement(16641..17243)
                     /locus_tag="OO575_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6664731.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17551..18690
                     /gene="arnB"
                     /locus_tag="OO575_001738"
     CDS             17551..18690
                     /gene="arnB"
                     /locus_tag="OO575_001738"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6664732.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18694..19662
                     /gene="arnC"
                     /locus_tag="OO575_001739"
     CDS             18694..19662
                     /gene="arnC"
                     /locus_tag="OO575_001739"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6664733.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19662..21643
                     /gene="arnA"
                     /locus_tag="OO575_001740"
                     /pseudo
     CDS             19662..21643
                     /gene="arnA"
                     /locus_tag="OO575_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21640..22530
                     /locus_tag="OO575_001741"
     CDS             21640..22530
                     /locus_tag="OO575_001741"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6664734.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22530..24182
                     /gene="arnT"
                     /locus_tag="OO575_001742"
     CDS             22530..24182
                     /gene="arnT"
                     /locus_tag="OO575_001742"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6664735.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24179..24514
                     /gene="arnE"
                     /locus_tag="OO575_001743"
     CDS             24179..24514
                     /gene="arnE"
                     /locus_tag="OO575_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6664736.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24514..24900
                     /gene="arnF"
                     /locus_tag="OO575_001744"
     CDS             24514..24900
                     /gene="arnF"
                     /locus_tag="OO575_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6664737.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24894..25166)
                     /gene="pmrD"
                     /locus_tag="OO575_001745"
     CDS             complement(24894..25166)
                     /gene="pmrD"
                     /locus_tag="OO575_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6664738.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25271..26626)
                     /gene="menE"
                     /locus_tag="OO575_001746"
     CDS             complement(25271..26626)
                     /gene="menE"
                     /locus_tag="OO575_001746"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6664739.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26623..27585)
                     /gene="menC"
                     /locus_tag="OO575_001747"
     CDS             complement(26623..27585)
                     /gene="menC"
                     /locus_tag="OO575_001747"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6664740.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27585..28442)
                     /gene="menB"
                     /locus_tag="OO575_001748"
     CDS             complement(27585..28442)
                     /gene="menB"
                     /locus_tag="OO575_001748"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6664741.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28457..29215)
                     /gene="menH"
                     /locus_tag="OO575_001749"
     CDS             complement(28457..29215)
                     /gene="menH"
                     /locus_tag="OO575_001749"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6664742.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29212..30882)
                     /gene="menD"
                     /locus_tag="OO575_001750"
     CDS             complement(29212..30882)
                     /gene="menD"
                     /locus_tag="OO575_001750"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6664743.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30971..32266)
                     /gene="menF"
                     /locus_tag="OO575_001751"
     CDS             complement(30971..32266)
                     /gene="menF"
                     /locus_tag="OO575_001751"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6664744.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32345..32650)
                     /gene="elaB"
                     /locus_tag="OO575_001752"
     CDS             complement(32345..32650)
                     /gene="elaB"
                     /locus_tag="OO575_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6664745.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32705..33166)
                     /locus_tag="OO575_001753"
     CDS             complement(32705..33166)
                     /locus_tag="OO575_001753"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6664746.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33231..>34007
                     /gene="rbn"
                     /locus_tag="OO575_001754"
     CDS             33231..>34007
                     /gene="rbn"
                     /locus_tag="OO575_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6664747.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAR"
BASE COUNT         8260 a         9162 c         8476 g         8109 t
ORIGIN      
        1 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
       61 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      121 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      181 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      241 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      301 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      361 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      421 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      481 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      541 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      601 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
      661 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
      721 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
      781 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
      841 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
      901 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
      961 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1021 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1081 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1141 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1201 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1261 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1321 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1381 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1441 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1501 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1561 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1621 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     1681 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     1741 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     1801 cgcgccagtt cgccagtcta acctgtgcct agaaattgcc ctgccgacca aaccgctgaa
     1861 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     1921 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     1981 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2041 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2101 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2161 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2221 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2281 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2341 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2401 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2461 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2521 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2581 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     2641 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     2701 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     2761 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     2821 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     2881 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     2941 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3001 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3061 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3121 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3181 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3241 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3301 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3361 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3421 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3481 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3541 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3601 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     3661 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     3721 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     3781 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     3841 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     3901 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     3961 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4021 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4081 gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga caccgacgat
     4141 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4201 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4261 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4321 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4381 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4441 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4501 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4561 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4621 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     4681 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     4741 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     4801 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     4861 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     4921 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     4981 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5041 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5101 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5161 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5221 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5281 actaatagtg cgcccagcag ataaaatgtg aatttaatca gtcggataca gcgatgaatt
     5341 ttattcgaca gggattaggc atcgccttac aaccagagtt aacgctgaaa agcattgcag
     5401 gtgaattgtg ttccgttcct cacgaaccaa ccttctatcg acagatttcg ttgctgacta
     5461 aagaaaagcc ggtagaaggc agtccactgt ttttactaca aatgtgcatg gaacaattag
     5521 tggcgattgg aaaaatttga tatgagacag gatggcgcat gaacgccatc ctgcagtaac
     5581 attactcttt attaagaaat tttactgcct tatcagggaa atcagtaaac agcccattta
     5641 cacccgcttt gttatacaga gcatcatata actgattcac atcaggagtg tattcaggca
     5701 gtttatctga ccgcacggta taaggatgca ctaccagttt attctgctgg gcatcttgca
     5761 ccatgccagt gagtttgata ttacccggct gcgatgtctc ctcaatcaac atatggtaat
     5821 ccggaccaat accatctgca tattccgcca cctgtttcat ggctcccggc ttaaacatcc
     5881 agtcgtagtt gtaattaacc cagcttccat ccggctgttt ctgctgcgtt tcattccagt
     5941 cggtataggc aatcagctgt accagattga gctccatgcc cattttgggt tccagctcat
     6001 tcttaatacg cttcagctca tcagcatcaa aacattgcaa ataaacttta tcgtctttac
     6061 cggtgtaacc atatttcttc agcacttcca gcgtttttgc cgcaatatcc ttcccttcct
     6121 gatgatggaa ccacggcgct ttgatttctg gatagatacc gatatttttc ccggtagagt
     6181 gatttaaccc ctgaacaaat tcaatctctt cttcaaaggt gtgcacccgg aagtcggact
     6241 tacccattgg gaaacgcccc ggataagtct gtactttttt accgttttca atatcgaaac
     6301 cttcggtaaa tttcagcgac ttaatttcat ccagcgtgaa atctatcgcg tagtaacgac
     6361 cgtctttgcg cgcccgatcc gggaaacgat cggcaacatc agtaacacga tcgagataat
     6421 ggtcatgcag aacaaccaga tggtcgtctt tggtcatcac caaatcctgt tccagataat
     6481 ccgctccctg cgcatacgcc atcgcttttg ctggcagcgt atgctccggc aaatatccac
     6541 tggcaccgcg atgggcgatg actatttttt cgttgctgtc cgccgccatt gcactgcttc
     6601 ccatgactat agtgctcatc atgatcgcca tgctaaggtt tttcagcttc aatttcatgc
     6661 cattagcctc cgttgcgttt ttgcagtaat tgttcatggc gacgtttttc gccaatcatc
     6721 acaacaatca acaagataac cgccagaatg ctgccgccaa tcattaccat aaagccgcca
     6781 tcccagccga agaagtccac ggtgtagcca acaatcgcgc tcgccgccac cgaaccgccc
     6841 aggtaaccaa acagcccggt aaagcccgct gccgtacctg ccgctttttt cggtgccagt
     6901 tccagcgcat gcagaccgat cagcatcaca ggaccgtaga tcaggaagcc gataacaatc
     6961 atacaaatca tatcgacggt tgggttacct gccgggttca tccagtaaac gatagtcgcg
     7021 atggtcacca gtgtcataaa gaagacgccg gttgccccac ggttaccacg gaagactttg
     7081 tccgacatcc agccgcacag cagggtgccc ggaatacccg catactcata gaagaagtag
     7141 gcccaggagg atttatccag cgcgaaatgc ttaacctctt tcagataagt cggtgaccag
     7201 tcgaggatgc cgtaacgcag cagataaacg aacacgttgg cgatggcgat gtaccacagc
     7261 agtttgttcg gcagcacgta ctgcatgaag atttgcttcg cggtcagctc ctgttccgct
     7321 ttttcgttat agtcgtccgg ataatcattt ttgtactctt cgatcggcgg caagccacag
     7381 gattgcgggg tatcgcgcat catcgcaaag gcgaataatg ccaccagaat ggcgcagaaa
     7441 gcaggcatat agagcgccgc atgccagtca ttgaaccagg ccatccccag caggaacagc
     7501 agcggcggaa taccaccacc gacgttgtgc gcacagttcc acactgacac aatgccgcca
     7561 cgttctttct gcgaccacca gtgcaccata gtacgaccac acggcggcca ccccatcccc
     7621 tggaaccaac cgcagaggaa caacagtaca aacatcaccg caatgctcga cgtcgcccat
     7681 ggcacaaagc ccataaacaa catcactgcc gccgccagaa tcaaacctgc gggcaggaaa
     7741 acgcgcggat tcgagcgatc cgataccgaa cccatgatga atttcgaaaa tccataagca
     7801 atcgagatcc ccgaaagggc aaaacctaaa tcaccgcgtg agaatccctg ctcaaccaga
     7861 taaggcatag caagcgcaaa gttcttacga accaaatagt aagccgcata gccaaagaat
     7921 atccccagga aaatttgcca gcgcaatcga cgataagtcg gatcgatctc cgcggcaggt
     7981 aagcgcgctt tgtgtggcgc tggtttaaaa atactcaaca ttgatagcct ccgtggcccg
     8041 tggtcttatt tatgattaac agcctgattc agtgagagaa cctgccgttt cttgagttgc
     8101 cgcgatgtta agaaaacatt cataaattaa atgtgaattg ccgcacacat tattaaataa
     8161 gatttacaaa atgttcaaaa tgacgcatga aatcacgttt tactttcgaa tcatgagcga
     8221 atatgcgcga aatcaaacaa ttcatgtttt tactatggct aaatggtaaa aaacgaactt
     8281 cagagggata acaatgaaaa ctcgcgactc gcaatcaagt gacgtgatta tcattggcgg
     8341 cggcgcaacg ggagccggga ttgcccgcga ctgtgccctg cgcgggctgc gcgtgatttt
     8401 ggttgagcgc cacgacatcg caaccggtgc caccgggcgt aaccacggcc tgctgcacag
     8461 cggtgcgcgc tatgcagtaa ccgatgcgga atcggcccgc gaatgcatta gtgaaaacca
     8521 gatcctgaaa cgcattgcac gtcactgcgt tgaaccaacc aacggcctgt ttatcaccct
     8581 gccggaagat gacctctcat tccaggccac ttttattcgc gcctgcgaag aagcagggat
     8641 cagcgcagaa gctatagacc cgcagcaagc gcgcattatc gaacctgccg ttaacccggc
     8701 actgattggc gcggtgaaag ttccggatgg caccgttgat ccatttcgtc tgaccgcagc
     8761 aaacatgctg gatgccaaag aacacggtgc cgttatcctt accgctcatg aagtcacggg
     8821 gctgattcgt gaaggcgcga cggtgtgcgg tgttcgtgta cgtaaccatc tcaccggcga
     8881 aactcaggcc cttcatgcac ctgtcgtggt taatgccgct gggatctggg ggcaacacat
     8941 tgccgaatat gccgatctgc gcattcgcat gttcccggcg aaaggatcgc tgctgatcat
     9001 ggatcaccgc attaaccagc atgtgatcaa ccgctgccgt aaaccttccg acgccgatat
     9061 tctggtgcct ggcgatacca tttcgctgat tggtaccacc tctttacgta ttgattacaa
     9121 cgagattgac gataatcgag tgacggcaga agaggttgat attctgctgc gtgaagggga
     9181 aaaactggcc cccgtgatgg cgaaaacgcg cattttgcgg gcctattctg gcgtgcgccc
     9241 gctggttgcc agcgatgacg acccgagcgg acgtaacgtc agccgtggca tcgtgctgct
     9301 cgaccatgct gaacgcgatg gtctggacgg atttatcacc atcaccggtg gcaaactgat
     9361 gacctatcgg ctgatggctg aatgggctac cgacgcggta tgccgcaaac tgggcaacac
     9421 gcgcccctgt acgactgccg atctggcact gcctggttca caagaacccg ctgaagttac
     9481 cttgcgtaaa gtcatctccc tgcctgcccc gctgcgcggt tctgcggttt atcgtcatgg
     9541 cgatcgcacg cctgcctggc tgagcgaagg ccgtctacac cgtagcctgg tatgtgagtg
     9601 cgaagcggta actgcgggtg aagtgcagta cgcggtagaa aatttaaacg ttaatagcct
     9661 gctggattta cgccgtcgta cccgtgtggg gatgggcacc tgccagggcg aactctgcgc
     9721 ctgccgcgct gccggactgc tgcaacggtt taacgtcacg acgtccgcgc aatctatcga
     9781 gcaactttcc accttcctta acgaacgctg gaaaggcgtg caacccatcg cctggggaga
     9841 cgcactgcgc gaaagcgaat ttacccgctg ggtttatcag ggactgtgtg gtctggagaa
     9901 ggagcagaaa gatgcgcttt gatactgtca ttatgggcgg cggcctcgcc ggattactct
     9961 gtggcctgca actgcaaaaa cacggcctgc gctgtgccat tgtcacgcgt ggtcaaagcg
    10021 cgttgcattt ctcatccggt tcgctggatt tgctgagcca tctgccagat ggtcaacccg
    10081 tggcagacat tcacagtgga ctggaatctt tgcgtcagca ggcacctgca catccttact
    10141 ctcttctcgg gccacaacgc gtgctcgatc tcgcttgcca ggcgcaggca ttaatcgctg
    10201 aaagcggtgc gcaattgcag ggcagcgtag aactggctca ccagcggatt acgccgctcg
    10261 gcactttgcg tgcaacctgg ttaagttcgc cagaagttcc cgtctggccg ctacccgcga
    10321 agaagatatg tgtagtgggg ataagcgggc tgatggattt ccaggcgcat cttgcggcgg
    10381 cttcgttgcg cgaactggat ctttcggttg aaaccgcaga aatagagctg ccggaactgg
    10441 atgtcctgcg caataacgcc accgaatttc gcgcggtgaa tatcgcccgt ttccttgata
    10501 atgaagaaaa ctggccgctg ttacttgatg cgcttattcc tgtcgccaat acctgcgaaa
    10561 tgatcctgat gcccgcctgc ttcggcctgg ccgatgacaa actgtggcgt tggttgaatg
    10621 aaaaactacc ttgttcactg atgcttttgc caacgctgcc gccttccgtg ctgggcattc
    10681 gtctgcaaaa ccagttacag cgccagtttg tgcgccaagg tggagtgtgg atgccgggcg
    10741 atgaagtgaa aaaagtgacc tgtaaaaatg gcgtagtgaa tgaaatctgg acccgcaatc
    10801 acgccgatat tccgctacgt ccacgtttcg cggttctcgc cagcggcagt ttctttagtg
    10861 gcggactagt agcggaacgt aacggcattc gcgagccgat tctcggcctt gatgtgctac
    10921 aaaccgccac gcggggtgaa tggtataagg gcgatttttt tgcgccgcaa ccgtggcagc
    10981 agttcggtgt aaccactgat gagacgctac gcccgtcgca ggcagggcaa accattgaga
    11041 acctgtttgc catcggttcg gtgctgggcg gatttgatcc catcgcccag ggatgcggcg
    11101 gcggtgtttg tgccgtcagt gctttacatg ccgctcaaca gattgcccaa cgcgcaggag
    11161 gccaacaatg aacgacacca gcttcgaaaa ctgcattaag tgcaccgtct gcaccaccgc
    11221 ctgcccggtg agccgggtga atcccggtta tccagggcca aaacaagccg ggccggatgg
    11281 cgagcgtctg cgtttgaaag atggcgcact gtatgacgag gcgctgaaat attgcatcaa
    11341 ctgcaaacgt tgtgaagtcg cctgcccgtc cgatgtgaag attggcgata ttatccagcg
    11401 cgcgcgggcg aaatatgaca ccacgcgccc gtcgctgcgt aattttgtgt tgagtcatac
    11461 cgacctgatg ggtagcgttt ccacgccgtt cgcaccaatc gtcaacaccg ctacctcgct
    11521 gaaaccggtg cggcagctgc ttgatgcggc gttaaaaatc gatcatcgtc gcacgctacc
    11581 gaaatactcc ttcggcacgt tccgtcgctg gtatcgcagc atagcggctc agcaagcaca
    11641 atataaagac caggtcgctt tctttcacgg ctgcttcgtt aactacaacc atccgcagtt
    11701 aggtaaagat ttaattaaag tgctcaacgc aatgggtacc ggtgtacaac tgctcagcaa
    11761 agaaaaatgc tgcggcgtac cgctaatcgc caacggcttt accgataaag cacgcaaaca
    11821 ggcaattacg aatgtagagt cgatccgcga agctgtggga gtaaaaggca ttccggtgat
    11881 tgccacctcc tcaacctgta catttgccct gcgcgacgaa tacccggaag tgctgaatgt
    11941 cgacaacaaa ggcttgcgcg atcatatcga actggcaacc cgctggctgt ggcgcaagct
    12001 ggacgaaggc aaaacgttac cgctgaaacc gctgccgctg aaagtggttt atcacactcc
    12061 gtgccatatg gaaaaaatgg gctggacgct ctacaccctg gagctgttgc gtaacatccc
    12121 ggggcttgag ttaacggtgc tggattccca gtgctgcggt attgcgggta cttacggttt
    12181 caaaaaagag aactacccca cctcacaagc catcggcgca ccactgttcc gccagataga
    12241 agaaagcggc gcagatctgg tggtcaccga ctgcgaaacc tgtaaatggc agattgagat
    12301 gtccacaagt cttcgctgcg aacatccgat tacgctactg gcccaggcgc tggcttaaac
    12361 tcccttctga tgcccggtaa gcatgtggtt accgggcatt tttgcgtaca cgattccgtg
    12421 cccaatgtat gcgttgcaac gcagtgaaaa ttcctctgaa aacgtctcgc aaaggctgaa
    12481 actggcagat gtcaaaggcc tgggataacc gtaatgtcgc gtcatcataa atatcaggtg
    12541 acggacaacc atgaccgaat caacaacctc ctccccgcat gatgcggtat ttaaaacctt
    12601 tatattcaca cccgaaaccg cacgggattt tctcgaaata catttaccag aaccactgcg
    12661 caagctttgc aacctgcaaa ccttacgcct ggaacccact agttttattg aaaaaagttt
    12721 acgcgcttac tactcggatg ttttgtggtc cgtggaaacc agcgacggtg acggctatat
    12781 ctactgcgtg attgaacatc aaagctctgc agaaaagaat atggcttttc ggccaatgcg
    12841 ctatgccact gccgccatgc agagtcacct agataaaggc tatgacagag ttccgctggt
    12901 ggtgccattg ctgttttatc atggcgaaac atcgccctac ccgtactcac ttaactggct
    12961 ggatgagttt gacgatccgc aacttgcccg gcagttgtac accgaagctt ttccgttggt
    13021 ggatatcacc atcgtacctg acgatgagat catgcaacat cggcgtatag ctctgctgga
    13081 actgattcaa aagcatattc gcgaccacga tttaatcggc atggtcgaca ggatcaccac
    13141 gcttttggtt agaggcttca ctaatgacag ccagctacaa acactgttta attatctgct
    13201 gcaatgcggc gatacctccc gtttcacccg ttttattcag gagattgccg aacgttcacc
    13261 actacaaaag gagagattaa tgactattgc tgaacggcta cggcaggaag gacatcaaat
    13321 tggctggcaa gaaggtaaat tagtaggttt gcagcaaggt aaattagaag gtttgcagga
    13381 aggtatgcat gaacaagcca ttaaaattgc tttgcgcatg ctggaacagg gcattgatcg
    13441 tgaccaagtg ctcgcggcca cccagctaag cgaagccgat ctggcagcga ataaccacta
    13501 attaacacag gcccacagcc gatccccatg ggcctttgat atcaataact accttttatg
    13561 cgtggcccat ttttgcctga tttaaacatc gccagtcgtt gatccagggc atcgctgtag
    13621 agcatcgtgt caacgccaac agcgacaaag ttcgctcccc acgccaggca ttgctgcgcc
    13681 atatcaggag ccacagccag aaaaccagcc gctttacccg cagcacggat ccgccgaatg
    13741 ctggtttcaa taattcgctg cacttccggg tgcccggcgt tatccgggta gcccaacgac
    13801 gcagaaagat ccgctggtcc aataaacacg ccatcaatcc cttcgacgtc gaggatctcg
    13861 tccaggttat ccagtgccgt tttactttcc acctgcacta acagacaaag cgaatcgttg
    13921 acttgcgcca tgtaattctc aatgcgtccc cagcgcgcag cccgtgccac actggcccca
    13981 acaccacgct caccgtaggg agggttggac aaattttgcc ggagggggaa tgtgccggtg
    14041 cagtaaaccg gcacagagtg gcgaaatcag acggataaag tcatggactc aacaacctca
    14101 atccagccat gctcactagc gatgtcctgg ccgtttaacc aacggcgtag catattcagt
    14161 gccatcattg cgcacacttc ctgacggata gccaggctgt agcgtgtagt gctgaaacgc
    14221 acacgcagag cgaaagtgcc gtctggcgtg gctagcgcaa agttgagatg ctcgttctcg
    14281 aaacccgaaa cagccagtgc cagcccggca aaatggttgg cccgccgttc tgtaatccag
    14341 tgcgcagttt gcgccagggt ttcttcctgt gaaggaatca cttcacacgc cagcaatgga
    14401 gcacctgcgc gagaaagttg caaagccaat aaaccaccgg tgaattgctc gctcaacgtc
    14461 aggctgaact ggcggttttg caattcgcga ctgatctgcg cgggcagtcc ttcggtgcct
    14521 tcgaaaatca cgctctgtcc ggcaacacgt ttaacgtcca gccacagttt ttccatcgcc
    14581 tgttgctcgc ttgccggtcc ggtgagtttc agttcgatga tcggcattga ggagcgatag
    14641 cccattgtta cgcccggcgg cagttgtaga gtgtccaggc tttgtgccag atcgctttcc
    14701 gaacgaccaa aggtagttaa acgcagacaa accggcggct gtggtaaaga aaaacgctcg
    14761 cgcaggcgcg gcaggatttc gtgctcgacc atcaccttaa attctgacgg tacgccggga
    14821 gtaaagaaca tcaggcaacg attaagctgc acggcaaaac cacaggcggt gcctaccggg
    14881 ttattgataa attcagcact ggcaggcagc tccgcttgtt tacggttgct cggtgccatt
    14941 actcgtccac gttcgtggaa atagcgttcc atctctttga gccaggcttc atgcagcacc
    15001 aggccttcac cttttgctgt cgcagcggcg agtgcgctta aatcatcgct ggtcggtccc
    15061 agcccgccgt taacgatcag cacatcggcg tgctgactac gttcgcgaag aatggtgact
    15121 aagtcatcaa ggttatcccc caccgtattg cggcgagata atggcagccc ctgatgaaag
    15181 aaaaaatcgg ccagccaggc agcgttagtg tcaacgattt gcccgtataa cacttcatcc
    15241 ccggtggata gcatttccac ttttaacatg gttatctcct gcttttgtta tcgaatcact
    15301 ataacgcaaa gggagataag gcgtagtaag aaactgaatg ccgcgccgga agggatcaga
    15361 aactggcgtt aaccccaaca tacaggccgt cagccacagc gttgtcgcgg ttaccgtctt
    15421 tacccgacag attcaggtag cgataacccg cctcaatact gactggacgc ataatggtgt
    15481 aacgcgcgcc agcattcgct tcctcataac tttgaatacc gctggagagc gaatccggag
    15541 agtagtaata ctcgccaaac aaacggaagc tgttgccaat tttccactgc aaaccacctc
    15601 ctaccgctgc ggcataacct tcatcgccgt aattcgggtt ggtgtacacg ccttttccgc
    15661 caacggtcgc cattaacggc ccgagaggca gattcaaccc cagccccacg cccgcgacgt
    15721 caccgtcgtc gtcgttatgt gtccagttac cgcttaaagc caggcccgtc gattcagtcc
    15781 caaaaccgac accaatattg gtgtattctt tacccgcctg gccgctgatg ctcattgcat
    15841 tgaccgatgc agaaaccaac agcattccgg ccagacctgc tagcgcaatt tttttcatta
    15901 tcccctttcc cacaagcaat taatcaaaat tcccaaagct gcgggattgt actgcctggc
    15961 acagaggatt caatgagcga aaaaagggat ctagtcactt attgtaacta aatacttctt
    16021 aaataattaa acgccgaaaa aataaacaca ctttaccgat acacacaaaa ataataataa
    16081 gtgcagttat cattgcaaaa gagattactg tcactttcac atatttctga gttaccgtat
    16141 tctcatggtt tctttttatt tataaggaat acccctgtgc gacaacggac tattgtatgc
    16201 cctttgattc aaaatgatgg tgcttatttg ctgtgcaaaa tggccgacga tcgcggcgtt
    16261 ttccccggtc aatgggcgct ttcgggtggc ggcgtggagt ctggcgaacg aattgaagag
    16321 gcactacgcc gcgaaattcg cgaagaactg ggagaacagc tgcttttgac agaaatcacg
    16381 ccgtggacct tcagcgatga tattcgcacc aagacatatg cagatggtcg caaggaagag
    16441 atttatatga tttacctgat ttttgactgc gtttctgcca accgagaagt gaaaataaac
    16501 gaagagtttc aggactacgc gtgggtaaaa cctgaagatc tggtgcatta tgatttaaat
    16561 gttgccaccc gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg acagccatca
    16621 ctggctgtca gattgaaaat ttagtggtta acgaattccc catccagata aactttgcct
    16681 ttttccacat gcatgactaa accatccaga taatcaggtt taaatatcgc atcacgctta
    16741 tttttagcaa tatatgtcag gcaatgatta tgggtgaaaa taacgatatt tttatcaggc
    16801 gctttgcttt gtaagtcctt aattgcacta taaatctcat taccgcactg aagaagtcgt
    16861 ttatctaccg tcagcttttt acccgcggaa aaccaggtcg ccgactgaat ggtccggacg
    16921 gtattactgg aataaagatc gaaatcagag atgtcagcgc taaaagcgtt gcccagttca
    16981 cgggcatcct gggtaccttt aaccgtaata cctgttttat ctgacaagca ttgattggtt
    17041 gaacgatcgc aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt
    17101 ctggccaatg ttttattgtc gatacgtggc aaaccattgc tactccaggc ggcatgagta
    17161 cccagtccgg caattgcagc gagcgccagt aaaatgatga aatatttttt tgacttcaat
    17221 gatgagcggc aaaaagctaa cacgtaaact ccacctatag acaagcgcaa ccagacaatt
    17281 accgtgaaat tgagcgacat ttctggcgat aattagcagt tggtgtaata ttaaaaatcc
    17341 tacgatgtcg gcaaaatgcc tcaaattttt gccaaatgca aagcctaaat aagaaaaaat
    17401 ataaaaattt caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc
    17461 cttaaaattt cgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg
    17521 gacaggtatt caatggcgga aggaaaagca atgtcagaat ttttgccttt ttcgcgacca
    17581 gcaatgggcg tggaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca
    17641 accggtccga agaatcaggc gcttgagcaa gccttttgcc agttgacggg aaatcagcat
    17701 gccatcgcgg tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt
    17761 ggcaagggcg atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt
    17821 tccttgctgg gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg
    17881 cctgaagcga tcgaatcagc cattacgcca cgcactaaag ccatcattcc ggtgcattat
    17941 gccggtgcgc cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca
    18001 gttatcgaag atgctgccca tgccgtcggt acgtattaca aagggcgaca tattggcgca
    18061 aaaggtaccg ctattttttc atttcatgcc attaaaaata ttacctgtgc tgaaggtggc
    18121 ctgattgtaa ctgataatga aaaccttgcc cgccagctac ggatgctgaa atttcacggt
    18181 ctgggtgtcg atgcctatga cagacaaacc tggggccgtg caccgcaggc tgaagtctta
    18241 acaccgggct ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta
    18301 gtcaaattag agcacctcaa cacccgtcgg cgcgaaattg cccagcaata tcagcaagca
    18361 ctggcagctc tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg
    18421 catctgttta ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg
    18481 gaagcgttaa aagaaagagg cattggtacc gggttacatt tccgcgccgc tcacacacaa
    18541 aaatattatc gcgagcgttt tcccacgctg tcgttaccga ataccgaatg gaatagcgaa
    18601 cgcatctgtt cgttgccgct gttcccggat atgactaccg ccgatgccga ccgcgtcatc
    18661 acagcccttc agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa
    18721 gtctcggtgg ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc
    18781 accaccacag cctgtgaatc gttggggaaa gagtatgaga tcctgctgat tgatgacggc
    18841 agtagcgata attccgcgca tatgctggtc gaagcctcac aagcggagaa cagccatatt
    18901 gtgtctattt tgattaaccg caattacggg caacattcag cgattatggc gggattcagt
    18961 cacgttactg gcgacttaat tattaccctt gatgccgatc tccagaatcc gccagaagaa
    19021 atcccccgcc tggtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag
    19081 aaccgccagg acagctggtt tcgtaaaaca gcttcgaaga tgattaaccg gcttattcag
    19141 cgcaccactg gcaaagcgat gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat
    19201 attgtcgatg cgatgttgca ctgccatgaa cgcagcacct ttatcccgat tctggcgaat
    19261 atcttcgccc gccgtgcaat tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa
    19321 tccaaataca gttttatgcg cctgattaat ttgatgtacg acctggtgac ctgccttacc
    19381 acaacgccgc tacgtatgct gagtctgctc ggcagcatta ttgcgattgg aggttttagc
    19441 attgcggtgc tgctggtgat tttacgcctg accttcggac cacaatgggc ggcagaaggc
    19501 gtctttatgc tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga
    19561 ttactcggtg aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt
    19621 gttcagcaag ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg
    19681 cctaccacga tatgggatgc ctcggtattg aagctctgct ggctgccggt tacgaaatta
    19741 gcgccatttt tacccatacc gataatcccg gtgaaaaagc cttttatggt tcggtggctc
    19801 atctggcggc ggaaagagac attccggttt atgcgccgga taacgttaat catccgctgt
    19861 gggtggaacg cattgcccaa ctgtcaccag aggtgatttt ctctttttat tatcgccatc
    19921 ttatttgcga cgagattttt cagctcgctc ccgcaggtgc atttaatctg cacggttcgc
    19981 tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg
    20041 aaacgggcgt tacattgcac cgaatggtaa acgtgccgat gctggggcca ttgtggcgca
    20101 actgcgcgtt gccattgcgc cagacgatat cgctattacg ctgcatcata aattgtgcca
    20161 tgccgcgcgc cagctactgg aacagacatt acccgccatt aaacacggta atattctgga
    20221 aatcgcccag cgcgaaaacg aagccacctg ttttggtcgc agaacgccgg atgacagttt
    20281 ccttgaatgg cataaaccgg catccgtact gcacaacatg gtacgtgccg ttgccgatcc
    20341 gtggccgggt gccttcagct atgttggcaa tcagaaattc accgtctggt cgtcgcgtgt
    20401 tcatcctcat gccagcaaag cacagccggg gagcgtgatt tctgttgcgc cactgctgat
    20461 tgcctgtggc gatggcgcgc tggaaatcgt caccggacag gcgggcgacg gcattactat
    20521 gcagggctcg caattagcgc agatgctggg cctggtgcaa ggttcacgct tgaatagcca
    20581 gcctgcctgc accgcccgac gccgtacccg ggtactcatc ctcggggtga atggctttat
    20641 tggcaaccat ctgacagaac gcctgctgcg cgaagatcat tatgaagttt acggtctgga
    20701 tattggcagc gatgcgataa gccgttttct gaatcatcca cattttcact ttgtcgaagg
    20761 cgatatcagt attcattccg aatggattga gtatcacgtc aaaaaatgtg atgtcgtctt
    20821 gccgctggtg gcgatagcca cgccgattga atatacccgc aacccgctgc gcgtatttga
    20881 actcgatttt gaagagaatc tgcgcattat ccgctactgc gttaagtacc gtaagcgaat
    20941 catcttcccg tcaacttcag aagtttatgg gatgtgtagc gataaatact tcgatgagga
    21001 ccattctaat ttaatcgtcg gcccggtgaa taaaccacgc tggatttatt cggtgtcaaa
    21061 acaattactt gatcgggtga tctgggccta tggcgaaaaa gagggtttac agttcaccct
    21121 cttcctcccg tttaactgga tgggaccacg actggataac cttaatgcag cgcgaattgg
    21181 cagctcccgc gctattacgc aactcattct caatctggta gaaggttcac caattaagct
    21241 aattgatggc ggaaaacaaa aacgctgctt tactgatatc cgggacggta tcgaggcgtt
    21301 gtaccacatt atcgaaaatg cgggaaatcg ctgcgacggc gaaattatca acattggcaa
    21361 tcctgagaac gaagcgagca ttgaggaact gggcgagatg ctgctggcga gcttcgaaaa
    21421 acatccgctg cgccatcatt tcccaccgtt tgcgggcttt cgcgttgtcg aaagtagctg
    21481 ctactacggc aaaggatatc aggacgtaga gcatcgtaaa ccgagcatcc gcaatgccca
    21541 ccgctgcctg gactgggagc cgaaaattga tatgcaggaa accatcgacg aaacgctgga
    21601 tttcttcctg cgcaccgttg atcttacgga taaaccatca tgaccaaagt aggcttacgc
    21661 attgatgtcg atgcctttcg tggcacccgt gaaggcgtgc cgcgtctgct ggaaatcttg
    21721 agtaagcata atattcaggc cagcattttt ttcagcgtcg gcccggacaa tatgggccgc
    21781 catctctggc gactggtgaa gccacagttt ttgtggaaga tgctgcgctc aaacgcggca
    21841 tcgctttatg gctgggatat tttactggca ggtacggcct ggccaggtaa agagattggt
    21901 catgccaatg ccgatatcat tcgtgaagcg gctaaacatc acgaagtcgg tctgcacgcc
    21961 tgggatcacc atgcctggca agcccgtagc ggtaactggg atcggcaaac aatgatcgac
    22021 gatattgcac gcggtcttcg cactctggaa gagattatcg gtcaaccggt aacctgttct
    22081 gccgctgcgg gctggcgtgc cgaccagaag gtgatcgaag caaaagaagc gttccatttg
    22141 cgctacaaca gcgattgtcg tggggccata ccgttccgtc cactgcttga atcaggaaac
    22201 cctggcactg cgcaaattcc ggtgacctta cccacctggg atgaagtgat tggtcgggat
    22261 gtgaaagcag aagattttaa cggttggtta ctcaaccgca tcctgcgaga taaaggcacg
    22321 ccggtttata ccattcatgc agaagttgaa ggctgcgctt atcagcataa ttttgtggat
    22381 ctcctcaaac gcgcagctca ggaaggcgtg acattttgcc ctttaagcga actgttatca
    22441 gagacgttgc cgctcggaca agttgttcgc ggaaatattg ccggacgtga aggctggctg
    22501 ggttgccaac aaattgcggg tagtcgctga tgaaatcggt acgttacctt atcggcatct
    22561 tcgcgtttat tgcctgctat tacctgttac cgatcagcac gcgtctgctc tggcaacccg
    22621 atgaaacgcg ttatgcggaa atcagtcgag aaatgctggc atccggcgac tggattgtgc
    22681 cccatctgtt agggctacgt tatttcgaaa aacccattgc cggatactgg attaacagca
    22741 ttgggcaatg gctatttggc gcgaataact ttggtgtgcg ggcaggcgtt atctttgcga
    22801 ccctgttaac tgccgcgctg gtgacctggt ttactctgcg cttatggcgc gataaacgtc
    22861 tggctctact cgccacagta atttatctct cattgtttat tgtctatgcc atcggcactt
    22921 atgccgtgct cgatccgttt attgcattct ggctggtggc gggaatgtgc agcttctggc
    22981 tggcaatgca ggcacagacg tggaaaggca aaagcgcagg atttttactg ctgggaatca
    23041 cctgcggcat gggggtgatg accaaaggtt ttctcgccct tgccgtgccg gtattaagcg
    23101 tgctgccatg ggtagcgacg caaaaacgct ggaaagatct ctttatttac ggctggctgg
    23161 cggttatcag ttgcgtactg acggttctcc cctggggact ggcgatagcg cagcgggagc
    23221 ctgacttctg gcactatttt ttctgggttg agcatattca acgctttgca ctagatgatg
    23281 cccaacatag agctccgttc tggtactacg tgccggtcat cattgccggt agcctgccgt
    23341 ggctgggatt actccccggt gcactgtaca caggctggaa aaaccgcaag cattccgcaa
    23401 ccgtctattt gttgagctgg acgataatgc cgctgctgtt tttctccgtc gctaaaggca
    23461 aattgcccac ctatattctt tcttgctttg cacctctggc aatgctgttg gcgcattacg
    23521 cgttgctggc agcaaaaaat aatcctctgg cgctgcggat taatggctgg attaacatcg
    23581 cttttggcgt cactggcatt attgccacat ttgtgatctc cccctgggga ccaatgaaca
    23641 cgccggtgtg gcaaaccttc gagagctata aagtcttttg tgcctggtcg attttttcgc
    23701 tatgggcatt tttcggctgg tacaccttaa caaacgtcga aaagacctgg tctttcgccg
    23761 cgctttgccc gctggggctg gcgttgctgg taggattttc aattcctgac agagtcatgg
    23821 aaggaaaaca tccgcaattt tttgtcgaga tgacacaaga atcacttcag ccaagccgct
    23881 atattcttac tgatagcgtc ggtgttgccg caggtctggc atggagcctg caacgcgatg
    23941 acatcatcat gtatcgccag acaggtgagt tgaaatacgg ccttaattat ccggatgcga
    24001 aagggagatt tgtcagcggt gatgagttcg caaactggct taatcaacat cgtcaggagg
    24061 ggattattac actcgtgctt tcggttgacc gcgatgaaga tatcaacagt ctcgccattc
    24121 cgcccgcaga tgccatcgtt cgtcaggagc gtctggtgct gattcagtat cgtcccaaat
    24181 gatctggcta acattagtct ttgccagctt gcttagcgtt gccgggcagt tgtgtcagaa
    24241 acaggcaacc tgctttgtgg cgataaacaa acggcgcaaa catatcgtgc tgtggctggg
    24301 actggcgctg gcttgtcttg gtcttgccat gatgctctgg ctgctggtct tgcagaacgt
    24361 accggtaggc attgcctacc cgatgttaag tctgaatttt gtctgggtga cgctggctgc
    24421 agtaaaactg tggcacgaac cggtatcgcc gcgtcactgg tgtggggtgg cgttcattat
    24481 tggtggcatt gtgatcctcg ggagtacggt gtaatgtgcc tgatatgggg attattcagc
    24541 gtcattattg cctcagttgc gcaattaagc ctgggttttg cggcaagtca tctgccgccg
    24601 atgacgcact tatgggattt tatcgcggct ctgttagctt ttggcctgga tgcccggata
    24661 ctgttactcg ggctgctggg ttatctgctt tccgtttttt gttggtataa aacgctacat
    24721 aaacttgcgc taagcaaagc ctatgccttg ctcagtatga gttatgtgct ggtatggatt
    24781 gcttcaatgg tacttcccgg ctgggaaggg actttttcgc tgaaagcact actgggagta
    24841 gcctgtatta tgagcgggtt gatgctgatt ttcctgccca cgacaaaaca acgttactga
    24901 gttttccctg ccgctgtaca ctgccgttcc cactcatcgg ggctataact gcttgccgag
    24961 aggactttca ccgtatgcag cttttcacga ttaatacaat aaagcgcatt ctgcaaaggc
    25021 gagagtaaat cccccacttt cacggcgaag tcactcttca cttcggcgat catttttatc
    25081 gcgccgccag catcgcagag cataagtaca tgtctgttat cttgtttatt gcagcacgat
    25141 tttttgtgcc agccattcca ttgcattatc ctgtttgcta agagttttca catcaattta
    25201 cagattaaca gataaactct ttatcaacgg caatggaatg gcagaaatga gaagtgaaac
    25261 ggagggttaa ttattgctga cgttgcaccc actcttttag cgcctgacgt gaaattttaa
    25321 taccgccgtt tttcagctcc ggcggtaaag tcagccagcg caccggttgt tgaaaacgag
    25381 ccagcttatc tttcacccat tcgccaagat caacggtttg ctggtcatac tccaccaccg
    25441 ctaccggccg atgaccaaac tccttgtcgg caacggaaac gataaacacc tgcaaaaccg
    25501 caggatgtgc agcaataaca cgctcgactt cttccggctg aataccctca ccgccactga
    25561 agaacagatt gtctaaacgt ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat
    25621 cgcgcgtagc gtaccagcct tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt
    25681 aaccttctgc catactggca gcccgcagcc acacttcatt attaacgatt ttcacttccc
    25741 gacccggcag cggcaaacca acgtctgcca ggccgtcggc ttctttcgca cataccgtgg
    25801 aggcaaactc ggtcagacca tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt
    25861 ccgtcaactc gaccgggata gccgcgccgc caagtaacac cgctttcagg gaaacggaac
    25921 tgcggttaac cagcaaacgc cagagttgtg ttggcaccag tgaagcgtga gtacagcctg
    25981 ccagcatttg ctccaatggc tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc
    26041 agcgccacat aattccctga ccggagacgt gaaacagcgg taaagagagc aaccagtcat
    26101 cgtgatcgcc aaacggaatc agcgataaca caccttgcgc actggcaaga tgggcctgat
    26161 aagtatggac agcggctttc ggcaaaccgg tagaacccga ggtcaacgtc attgagcaca
    26221 gacgcgtcgg ctgccacgca gcggcatgta cgccttcaac cagctgaatg tgcagcgacg
    26281 ttaatgccgg aaacgtgttt tccccatccg gcaccagcgc aaattgcagc gtcagattgg
    26341 gcagcaattc ttcaagcaac ggttgcggca gctgagggtt cacgggcaac acccgcgccc
    26401 cgcattgcag taacgccagc caggcgagca gcgtttgcgg cgtattccac gcccgcaaca
    26461 tcacgccgct gccctcaacc accccctgcg ccgcaaagcc cgtagctaac tcatcgacgc
    26521 gagcacaaag ctcgcgccag ttgagttgct cgtcattaag acgtaaggcg atggcttctc
    26581 cccgcacttg ccgccagtga cgccacggcc agtcagagaa gatcataaca actgctcctg
    26641 tacatcagcg tccactaacg gcagtgggct ccccggccag cgacgaacct gctgcgcctg
    26701 catcagatcc agcgtgtcca gtcccggaat ggtgtctggc gttaaccagg cagcaatccg
    26761 cgccagttgc gttagaccta agctcgactc aatggaagaa ctgatcaccg ccgtcagccc
    26821 cagcgcatgc gccgcctgta cctgctcgcg tactttttcc agactgcccg tgagcgtggg
    26881 tttgataact accgcgcgca cgccctcttc agccacaaag gcaaaatccg gctcgcgcag
    26941 gctttcatcc caggcaatgg caatgccggt ttcacgggca aacgctcgcg aatcatcgcg
    27001 ggttttgcac ggctcttcga gaaacgcgat gcggtggcga taatccgggt taacatattt
    27061 ggcaaactgc tgacctttca gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag
    27121 atccggaatt gcctccagca acagattcac caccatgccg tcgcgcaccg cttcgtacaa
    27181 tcccactttg accttcgcca ctttctcgcc cggcatatcg gcaagtttga ggatcagatc
    27241 gtccggatcg ccattacaca gcggtgccgc acggtagttg gctgcttgcg gcaacgtatc
    27301 tgtcagttct gccaatgcac agcttacgcc aaaggccacg gaaggcatct gcggtagctc
    27361 gcaatcgcct gccagccagt tatttaccca ggcaagcagc acgctttgcg cctcttccca
    27421 gttttcctgg ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcgccctc
    27481 acgcaggcaa acgtacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc
    27541 cgcgtccatg gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta
    27601 aatttgctga aatcaggctg acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct
    27661 tccgtcatgt agaacagcat ggtggcgttg cccgccagct cctgcagccc cgcctgcccg
    27721 tcacagtcgg cgttcagcgc agctttcagg cagcgcagcg ccatcgggct gttttgcagc
    27781 atttcgcggc accaacggac ggtttctttt tccagatccg ccagcggtac cacggtgttg
    27841 acaaggccca tatccagcgc ctgttttgcg tcgtactgac ggcacaggaa ccagatttct
    27901 cgcgcttttt tctgcccgac gatacgagcc atgtaggaag cgccccagcc gccgtcgaag
    27961 gaaccgactt tcgggccagt ctgaccgaag atggcattat ctgctgcgat agtcaggtcg
    28021 cacatcatgt gcagaacgtg accaccgccg atggagtagc cagccaccat cgcgacaacc
    28081 ggtttcggac aggtacggat ctgacgctgg aagtccagca cgttcaggtg atgtacgccg
    28141 gaatcatctt tatagccgcc gtaatcacca cgcactttct ggtcaccacc ggagcagaac
    28201 gctttatcgc ctgcgccagt cagaatgatc acgccgatat tgtcgtcata acgcgcatct
    28261 gccagtgcct ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga
    28321 cgattaatgg tgatttttgc gataccgtcg gtggattttt cataacgaat gtcctcgaaa
    28381 ccttcggagc agtcgtgcca ttcaaccggt gcgtaaggca ttgcttcatc aggataaatc
    28441 atattgtgtc ctttagtcag aaacgcaaga tctgcgccag acttgcgatt acgccagcgg
    28501 gattttcccg atgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt
    28561 ccgccgccag ggcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg
    28621 tgcgggcgct aaggttagca cgtaaatcag gctggacggc gagagaagtc gcctccagca
    28681 tggcggcaag ggttgcgcca ttattgttgc tgcgcagcgc caccagctcc cggcgttgat
    28741 cgtcattgag cgaggcaaaa acaggctgtt gataccagtc ggcaaatacc gccgttaacg
    28801 gttctgtgcg aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcacgttgtt
    28861 cagcattttg cagccccgga tgcccgcctt cgacaataac cccacaaagc cccgccagcc
    28921 cctggcaagc cgccatcatc gccacccgtc cgccaagcga gtaccccacc agccagaagt
    28981 caaggatgtt gtaactaacc aaggttttac gcagtaagtc ggtgacatca tcaaatccat
    29041 cgacgctaat cgccgccgaa ccaccatgac ctaggagatc aacatacaac cgtgagtagt
    29101 cggcaaacgc ctcgcccact tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa
    29161 acaccagcca gggtaaacct agttttccgt gttttgcctg cgcgtgcagg atcataaatg
    29221 gcttacctgc gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat
    29281 ttcaatcacc gtggtggttg gcgtgcgcca ggcgtcggca aatgccgttt caagttcctg
    29341 ccagttttgc ggatgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac
    29401 gttttgcggc atcagataga aacgctcacg ctcgctttgc ggcgttggca acagcgagaa
    29461 aatttgcccg ccgttgttgt tcaccacaat taataccagc ggcgcagaaa cctgacgcaa
    29521 taacgccagc gcgttgagat cgtaaagcgc ggagagatcg cccacaatcg ccagcgtcgg
    29581 tttgccgctt gcccgctgaa cgcctgcggc ggtcgaaagc agtccgtcga taccgctggc
    29641 ccctcggttg ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg
    29701 taccaccagg ctgttaccaa caaacaattg cccctgttca ggcagatagt cgctgatgcg
    29761 atgcgccagt tgcgcttcgc caaacgcatc acggcgggca ataaccgcct gcattgcctg
    29821 ttccgccagg cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc
    29881 cagccagtcg gcaatattgg caattaagcg acgtccgcga tggtgtgccg gatcaagtcg
    29941 cccttcaatg tcatcaacaa tccagtactc ttctggttca cagcttgcct gccattgcag
    30001 gagccgtttc cccgtcaggc tacttcccag ttgcaccaca atttgcgcct gctgcagctc
    30061 gctggtcgct ttggcattgc ctaaccaaag atcggcacac ggcagcggct gcccggtttg
    30121 tgacagcaca tcgccaatca gcggccagcc aagagtttgc gcccacaggg caactttttt
    30181 gccctcttcc gcactcatgc gcccggcaac caccacgccg cgcttttgtc gccagaagaa
    30241 ccagtcgcgc tgtttttcac tttccagacg aggcgcttca cgcagccacg gtttgtcgtc
    30301 ctgccaccag tcacccagac gctgttgcca gctaagcccg gtatcgtcca tttcgccata
    30361 cagcggttca gcaaacgggc agttgatatg gactcccccc gcatgaagcg taccgagagc
    30421 gtggtcgatg gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga
    30481 aatactgtgc gtggggtgag aggcgaacat tcccggctgg cgaattgcct gattcgcgcc
    30541 gcagtcaatt agctccggcg ggcgatcggc ggttaagaga atcagttttt ctccggttaa
    30601 cccggcttca atcagtgccg gatagagatt tgccaccgcc gtgccggagg tcacaatcac
    30661 cgccaccggc tgcttgctga ctttcgccag ccccagcgcc agatgcccca acccacgctc
    30721 atcgaaatgg gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt
    30781 agaacgcgag cctggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag
    30841 aatgaccgcc gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa
    30901 tattgcggct aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg
    30961 cgactcatta ctattccatt tgtaataaag tacgcagccc tgccgctttg ttgtcgattt
    31021 cctgccactc ttgctcgggg tcggaaccac ggacaatgcc cgcgccagca tataatcgca
    31081 cgacattgcc gctaattttt gctgagcgca gggaaacgca gaattcgctt tgttgtaatg
    31141 agagatagcc cgcagaaccg gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa
    31201 actgtcgcgc cagatcgcgc ggtaagccag caactgctgc cgttggcagc aactgatgta
    31261 aacagatcac atcatccgct ttgttgagtg aagtccagat acagcggcga agatgctgca
    31321 ctttacgcag acgcagtacc tgcggcggta aaacatccag cgtctgggta tcggcctgta
    31381 atcgttggca gatatcttcc accaccagca tgttctcgcg ctggttttta tcatccgcca
    31441 tcagccactc tcctaactgc tgcgcctgct tatcatcagg atgatttgct actgttcccg
    31501 ccagcgcttc agtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagagc
    31561 caagaaaagc attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc
    31621 gacgactggc agccatcatc gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg
    31681 cccgagcgag caccactttg tcgagctcac cttcggcgat ggttttcgtt gccagttcga
    31741 ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta
    31801 acccaggcaa gggcttgata ctcaccagtg tggcgataaa ttcttttgcc tgaatcgcat
    31861 cgtgctgaag ggagctttcg ctgaatagcg tcagccgcag cgtggcttta ccgccacagc
    31921 gtcgccattc caggcggggt aaaagtaaat tgccctgcga cgggtcaaaa gcattcagcc
    31981 cccaaatgcg taagtcagcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca
    32041 acgacgtaaa acgggtaatc gcgcccagga cggcagcttc ttcatcacca ttacgttgtt
    32101 gccagtagaa ttgcgggtac gtttgctgac tggccagcca gctcaaggca tcaaaagcgt
    32161 ctttgagagg gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg
    32221 acaaatggcg cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct
    32281 gttaaaagcc tcacattata cggggtacta caaaaaaaat gcagtacccc ggtgtaggga
    32341 ggttttaacg gcgtgccagc aacagtccta gtaccagccc gacggccgcg cccacaccaa
    32401 ttccttgcca gggtttttcg tggacgtagt catcagcacg ataaacagcc tgcttcgccc
    32461 gatagtaata actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt
    32521 ctgcatgcgc tttcagctca acatatttct gatcggcggg atcgccagag gagcggagca
    32581 cctcttccag tgtttcacta agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact
    32641 gattagacat tctcgttctc catttgcgta aaacctgtca ctaactatag acaagagttg
    32701 ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat
    32761 agacctccgt cacagggata aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg
    32821 ccccgaggta aacaggctta tcaggccagt gatgcgtaca tgtttcgagt gttttactca
    32881 tcagctgctg ccccactttt tcaccacgca acgcttcact gacaatcacc cgacctataa
    32941 cgaccggctc aagatcatca tcacttttca gaatcctcgc atacgccacc agttcatcat
    33001 ttttccaacc gagaatatgg cgattatccc ccgtcaaatc atcaccatcg atatcctgat
    33061 aagggcaatt ctgctcgaca acaaacaccg cgcaacgcag ttgtaataag gcataaagct
    33121 gggaaaccga taattcagag tgatgcagat cttgccattc aatcataatg ctctcctttt
    33181 agatgtatgc cttatactag ccggaatatt ttttgaaacg tgatgaactc atggaattaa
    33241 tttttttagg tacttcagcc ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc
    33301 ttaacctgca acatccgacc cagagcggac tttggttgtt tgactgcggt gaaggcaccc
    33361 agcatcagct actgcatacc gcctttaacc ctggaaaact ggacaagatt tttatcagtc
    33421 accttcatgg cgatcatctt tttggtttac ccggcttgct gtgcagtcgt tctatgtcag
    33481 gcattatcca acccttaacg atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg
    33541 cgctgcggat tagcggctca tggaccgatt atccgctgga aattgtcgaa attggcgctg
    33601 gcgaaattct cgatgatggc ctgcgcaaag taaccgctta tccgctggaa cacccactgg
    33661 aatgttatgg ctatcgtatt gaagaacatg ataaaccggg tgcattaaat gcccaggcat
    33721 taaaagctgc tggcgtgccg cctggcccac tgtttcagga attaaaagcg ggcaaaacaa
    33781 tcacgctgga agatggaagg cagattaacg gcgcagatta cttagctgct ccagtgccag
    33841 gtaaagcgct cgctattttc ggcgataccg gcccctgcga tgccgcactt gacctggcta
    33901 aaggtgtcga tgtcatggtg cacgaagcga cgctggatat aaccatggaa gccaaagcca
    33961 atagtcgcgg ccatagctct acacgccagg ctgcgacact agcccgt
//
LOCUS       DAJTYM010000036        33764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTYM010000036 DAJTYM010000000
VERSION     DAJTYM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..248)
                     /locus_tag="OO575_001755"
     CDS             complement(<1..248)
                     /locus_tag="OO575_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6664748.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            864..1001
                     /locus_tag="OO575_001756"
     CDS             864..1001
                     /locus_tag="OO575_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664749.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1601..2380
                     /gene="ipaJ"
                     /locus_tag="OO575_001757"
     CDS             1601..2380
                     /gene="ipaJ"
                     /locus_tag="OO575_001757"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6664750.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3327..4256)
                     /gene="virB"
                     /locus_tag="OO575_001758"
     CDS             complement(3327..4256)
                     /gene="virB"
                     /locus_tag="OO575_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6664751.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4473..4709)
                     /locus_tag="OO575_001759"
     CDS             complement(4473..4709)
                     /locus_tag="OO575_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6664752.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4713..6614)
                     /gene="ipaA"
                     /locus_tag="OO575_001760"
     CDS             complement(4713..6614)
                     /gene="ipaA"
                     /locus_tag="OO575_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6664753.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6623..7621)
                     /gene="ipaD"
                     /locus_tag="OO575_001761"
     CDS             complement(6623..7621)
                     /gene="ipaD"
                     /locus_tag="OO575_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6664754.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7672..8763)
                     /gene="ipaC"
                     /locus_tag="OO575_001762"
     CDS             complement(7672..8763)
                     /gene="ipaC"
                     /locus_tag="OO575_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6664755.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8783..10525)
                     /gene="ipaB"
                     /locus_tag="OO575_001763"
     CDS             complement(8783..10525)
                     /gene="ipaB"
                     /locus_tag="OO575_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6664756.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10531..10998)
                     /gene="ipgC"
                     /locus_tag="OO575_001764"
     CDS             complement(10531..10998)
                     /gene="ipgC"
                     /locus_tag="OO575_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6664757.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11055..11681)
                     /gene="ipgB1"
                     /locus_tag="OO575_001765"
     CDS             complement(11055..11681)
                     /gene="ipgB1"
                     /locus_tag="OO575_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6664758.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11697..12086)
                     /gene="ipgA"
                     /locus_tag="OO575_001766"
     CDS             complement(11697..12086)
                     /gene="ipgA"
                     /locus_tag="OO575_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6664759.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12099..13583)
                     /gene="icsB"
                     /locus_tag="OO575_001767"
     CDS             complement(12099..13583)
                     /gene="icsB"
                     /locus_tag="OO575_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6664760.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13897..15513
                     /locus_tag="OO575_001768"
     CDS             13897..15513
                     /locus_tag="OO575_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6664761.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15523..15885
                     /gene="ipgE"
                     /locus_tag="OO575_001769"
     CDS             15523..15885
                     /gene="ipgE"
                     /locus_tag="OO575_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6664762.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15885..16343
                     /gene="ipgF"
                     /locus_tag="OO575_001770"
     CDS             15885..16343
                     /gene="ipgF"
                     /locus_tag="OO575_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6664763.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16352..17467
                     /gene="mxiG"
                     /locus_tag="OO575_001771"
     CDS             16352..17467
                     /gene="mxiG"
                     /locus_tag="OO575_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6664764.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSNEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQKYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17475..17726
                     /gene="mxiH"
                     /locus_tag="OO575_001772"
     CDS             17475..17726
                     /gene="mxiH"
                     /locus_tag="OO575_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6664765.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17739..18032
                     /gene="mxiI"
                     /locus_tag="OO575_001773"
     CDS             17739..18032
                     /gene="mxiI"
                     /locus_tag="OO575_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6664766.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18038..18763
                     /gene="mxiJ"
                     /locus_tag="OO575_001774"
     CDS             18038..18763
                     /gene="mxiJ"
                     /locus_tag="OO575_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6664767.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18760..19287
                     /locus_tag="OO575_001775"
     CDS             18760..19287
                     /locus_tag="OO575_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6664768.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19229..19924
                     /gene="mxiN"
                     /locus_tag="OO575_001776"
     CDS             19229..19924
                     /gene="mxiN"
                     /locus_tag="OO575_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6664769.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19917..20324
                     /gene="mxiM"
                     /locus_tag="OO575_001777"
     CDS             19917..20324
                     /gene="mxiM"
                     /locus_tag="OO575_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6664770.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20308..20736
                     /gene="mxiM"
                     /locus_tag="OO575_001778"
     CDS             20308..20736
                     /gene="mxiM"
                     /locus_tag="OO575_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6664771.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20866..21498
                     /gene="mxiE"
                     /locus_tag="OO575_001779"
     CDS             20866..21498
                     /gene="mxiE"
                     /locus_tag="OO575_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6664772.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21485..23185
                     /gene="mxiD"
                     /locus_tag="OO575_001780"
     CDS             21485..23185
                     /gene="mxiD"
                     /locus_tag="OO575_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6664773.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23204..24271
                     /gene="mxiC"
                     /locus_tag="OO575_001781"
     CDS             23204..24271
                     /gene="mxiC"
                     /locus_tag="OO575_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6664774.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24284..26344
                     /gene="mxiA"
                     /locus_tag="OO575_001782"
                     /gene_synonym="virH"
     CDS             24284..26344
                     /gene="mxiA"
                     /locus_tag="OO575_001782"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6664775.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26357..26758
                     /gene="spa15"
                     /locus_tag="OO575_001783"
                     /gene_synonym="spaK"
     CDS             26357..26758
                     /gene="spa15"
                     /locus_tag="OO575_001783"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6664776.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26762..28054
                     /gene="spaL"
                     /locus_tag="OO575_001784"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26762..28054
                     /gene="spaL"
                     /locus_tag="OO575_001784"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6664777.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            28147..28485
                     /gene="spa13"
                     /locus_tag="OO575_001785"
                     /gene_synonym="spaM"
     CDS             28147..28485
                     /gene="spa13"
                     /locus_tag="OO575_001785"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6664778.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28472..29350
                     /gene="spa32"
                     /locus_tag="OO575_001786"
     CDS             28472..29350
                     /gene="spa32"
                     /locus_tag="OO575_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6664779.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29344..30225
                     /gene="spa33"
                     /locus_tag="OO575_001787"
                     /gene_synonym="spaO"
     CDS             29344..30225
                     /gene="spa33"
                     /locus_tag="OO575_001787"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6664780.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30230..30880
                     /gene="spa24"
                     /locus_tag="OO575_001788"
                     /gene_synonym="spaP"
     CDS             30230..30880
                     /gene="spa24"
                     /locus_tag="OO575_001788"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6664781.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30895..31155
                     /gene="spaQ"
                     /locus_tag="OO575_001789"
     CDS             30895..31155
                     /gene="spaQ"
                     /locus_tag="OO575_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6664782.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31155..31925
                     /gene="spa29"
                     /locus_tag="OO575_001790"
                     /gene_synonym="spaR"
     CDS             31155..31925
                     /gene="spa29"
                     /locus_tag="OO575_001790"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6664783.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31932..32960
                     /gene="spa40"
                     /locus_tag="OO575_001791"
                     /gene_synonym="spaS"
     CDS             31932..32960
                     /gene="spa40"
                     /locus_tag="OO575_001791"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6664784.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32973..33215
                     /locus_tag="OO575_001792"
     CDS             32973..33215
                     /locus_tag="OO575_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664785.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10516 a         4918 c         6780 g        11550 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgtccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacacttgtt gactacctgt gcggcatctg
      361 gtatcaaggt ggcgtgaagc cggttcaggt tctcatgcgg aacgttggaa cctatatccc
      421 cccgatacca aaggcgaaat gtaagtgaca aaatgcaatg aacacgttct ccgttaactg
      481 aggagcatgg tgataaataa aggtaaatgc agaaggtgta gagtctcgca gatttctgac
      541 atcatagagc ctaacattag atgcataacg ctacagattg tagaatctct acaggtgaaa
      601 tatacactgg ttggcgttta tgtcgcaaaa catgtcattc aggttcatct caccaataag
      661 gatatgagtg aagtggagga taagtgagtc tgctgtcaga gttttctggt gtatgtcagt
      721 aactgcgaac catgtctgct tgatatggga gcttatggag gctctctgta caaggttagg
      781 gagctaacaa agcagggata aaaagtccgc ctgatgcagg cacggttcgt taaatctttt
      841 gccattggta acaaaatggt gcgatgaatg ttcaggctat ctttatcttg atggtggttc
      901 agctgtttgg taaagaaatc gctgtaacaa tagaagaact gcaggcagta ctggtcccac
      961 atcgtttgct cagtcaactg gtgaagttcc gtagcgcgta aattaatgct ttgcatgaaa
     1021 cgttactgga gtggatgaaa gaatacctgc atatatcatt actgcgttgg attaccagta
     1081 caacagattg aatgaattgg acatgttgat agacggcatt gaaaagcagc ttataagcat
     1141 agtgcggcag agttaattgg agagcacttc gccttctgag tattaagtaa gcatggtggt
     1201 acctgcggat actatctatt cactgtgctc ggtcggcagc cttactgacc aaggagttgg
     1261 gggagttgat aacaaagttg aaaaaacagc tctcaaccaa ggtggttatt gtaacaatag
     1321 caaataagct ggtgtgacct gtatcacaat aaccgattat ggacgtaaat acgacaaaaa
     1381 tatgtcagta tcaggcggta ttaattgctg atgctgatgg acattaactc ttaataaaag
     1441 ggtggatgct gaaaggttgt tatggtgacg gacgtgatga cgacggaaaa gagtatgagg
     1501 ttttggattt aaagttcgtt cggggaataa ggacccggtc agctaattct catagtagtt
     1561 cgcattgctg gatgcaatac tgtgctgcaa ttactcgtcg atgtcggaac aacggaagcc
     1621 ttgcaaacgg ggatgtattc atacaggtgt tatgttatat ggggttttat tgcagggggc
     1681 tataccaaga gaatatatga tatctcatca gactgatgtg agggtaaatg agaatagagt
     1741 aaacgaacaa ggttgttttt tggcgaggaa gcagatgtat gacaattctt gtggagctgc
     1801 cagtttactc tgtgctgcaa aggaattagg ggttgataaa ataccacaat acaaagggtc
     1861 tatgtccgaa atgacaagga agagttctct tgatttggat aatcgttgtg agcgggattt
     1921 atacttaata actagtggta attataatcc gagaatacac aaagataata tcgcggatgc
     1981 cggttattct atgcctgata agatagtcat ggctacgagg cttcttggat tgaatgctta
     2041 tgtcgttgaa gaaagtaaca ttttttcgca agtaattagt tttatttatc ccgatgcaag
     2101 ggacttatta attggcatgg gatgtaatat agtacatcaa cgagatgtgt tatcttctaa
     2161 ccaaagagtt ttggaagctg ttgctgtatc ctttattgga gttcctgtcg gcctccactg
     2221 ggtgttgtgt agacctgatg gaagctatat ggatccagct gtaggagaaa attattcttg
     2281 tttttcaact atggaattag gtgcaagaag aagtaattct aactttatag gatatactaa
     2341 aataggtatt tccatagtta taactaatga ggctttgtaa attgtcgttt tttatctgtg
     2401 aaggagcttg aatattttgg ggtggggggc ataacaaatg atattactga gtacaaattt
     2461 agctatattg ggcggattag ttgtgtggga agacgaaatg tttctcttga aataagtgaa
     2521 ctccctcaag gaatgattgt gtgcttaaat atttaagtgc tggtaagttt gagaaggctg
     2581 acatattttg cagagcaaac agtgattgac ttggatgata tttttatttt agtcactcgt
     2641 tgtaattgga gtatgcttta ttttatttga aaatagatgt attttatacc cgcaatttaa
     2701 actgtaagcc ctatgagggg tatggatatt ataagttact ctttgaccaa gcataacttt
     2761 tggggttaga aaaataaatc tcttgttaat aattaatatg attttaaaat caaacttgtt
     2821 tataattata aaataatgta agtcggtcat tttatgggaa gattaggaga ataaaacagt
     2881 aattatattt gagaaatctg ctaatatatt cttaaagctt ttttgatact gtgaacgaag
     2941 tctgttaatg gtggattttt tgtttatcac catgtaaaaa aaattgagta gtaaaagcgt
     3001 tgactgctaa tcctcgctgt ttttattgaa acgattaaaa atacgctgaa tattttcggc
     3061 cagtcactca tattcacata aaattttgac catcatgccg catcccttag ttgcacaatt
     3121 cattttcaat gatatgaata ttactaattt caccagataa ttttctcgtg gtcagcaggg
     3181 tatgagtttt caccgtaatt ttaaggaaac acagtgcgtg gggattgaat aacagatagc
     3241 cgacggaatt accccaccgg ctgaaagtca agttcaacga agatttttat tatctgaatt
     3301 gggcagttta catcagtgtt cgatgtttat gaagacgata gatggcgaga aattatatcc
     3361 cgaatagctt catctatttc tctctgaact gttttgttta ttttagataa tgtaaatatt
     3421 agagttctgt ttgttttatt ctcttttctt tttatgtaag tccgtttgtc tttagaaata
     3481 aataacgagt caacttttag cgaagggttt tgctttctgt ttttgctttt ctttatgatg
     3541 tttaaaattt cttttttata tatgtccggg ggcaaatttg tatcaaggtc ttttatttct
     3601 tcctttaata ttggtagtgt agaactaaga gattcattag ccttttcaag tcctttataa
     3661 taattgaata atatcttgta gtcattaaag ttaagttctg acgcgattgg aaatagagat
     3721 attatttcct gtggaacgct tgctgcctga aaggcacgag tgactttcgc gcgagacaga
     3781 ttctcttttt tggctatgtc tttataggac atccctgata ctttcagaaa attaagacca
     3841 ataccaagtt ctcggatgct atgctctttt gctgtttgta tatcgtttgc tagttttctg
     3901 gcatcaagag tagatatata ttcttttgaa tatagaactt caagatctgc tcctgcatat
     3961 attgcagatg ctcttctacg agtgccatcc agaatttcaa ttctaccatc aatctccctt
     4021 cctattacag ggaagaattg ttgtagcttt atagttttta tgatatcggc taatgattct
     4081 tctgttaggg attcttgatc gcgtacattc gttttttggt tgacgaaggt taaatcttgg
     4141 atccttttat gaggaatctt ggctttgata aaggaaactt tattaccaga atggagtgta
     4201 aactctttct tttggccttc ctttatactt aacaagtcgt tgcacaaatc caccatatca
     4261 caccctgttt attcatattg atttatattg acaaaagtta aatgcagtgt gagcaagatt
     4321 gtatgtaact gttgatgata gtcaacggta gtcttaaaaa ttactatttt aatagctgga
     4381 ttgatgcaat ttatctttat tggataaaag aataatattc ttgtggagct ctgatgtgag
     4441 agctccatag atgaaaaaat gcttatcaat tactatattg attttgtact atttttgact
     4501 acagaaataa gatctccaat agtaataatg tgttctaatg tagattcatc tatacgtaga
     4561 ttgaactcaa aactaatcat atccagcatt tcaatcagag aaagagaatc ggcagaaaga
     4621 tcttcaataa atttagtttc ttcagatagt ttgctgtacg ctataccata gcaaaaggcc
     4681 actattgata atattttttc ttttatcata ttttaatcct tattgatatt ctttaatact
     4741 tttgataggg aagtggttac atcttttgct gctttatata tattattgtt ttcagctgta
     4801 atatcattgt tattttttaa atcagatata tcatctgtaa gtaattctgc agaggtatcg
     4861 tcaatttttg acaatacctt gctgagggcg ctagatactt cttttgcctt ttcatagatc
     4921 gcatgatttt tatcaattgt atctgcgttg gtttctgtta cgtagtttct tatatttgca
     4981 tctgtaactt ttaatttgtt gtttattcta tgttctgttg tggattttgt aatgtccttt
     5041 gatttttcct gtaactctcg ttcttgtgtt cctgatttag ttccaatgag agatataaga
     5101 ttacttaggg agtttgttac tttttttgaa gcttcaaata tcgtttcccg tgttaaatat
     5161 gtgtctccat ttcttgataa aaaatcagtc tgaagtactt ttgaatcagt gtttgagttg
     5221 aatttattga tttgcgaagc tactctatga gaaagagaag cgctactttt agttgtatct
     5281 gatgagttat cattactgct tgtcactcct tccgggtgga gagggggggt ggttgaagag
     5341 ttctgtatgt tcagaggcct gtgtccccga gaaagagagt tatttgcaga tctttcttga
     5401 ctactcgtag ttaacaggtc tgaacctatt ccatccgaat tatctggagt atggttgaaa
     5461 gccttttcta aagctgaatt atttgcaaat gtctggacag tatctgatat agagtgtggt
     5521 agaacggaag ttactttttg aactagtgct ctttgtggat ttagtaatga gtttccgtta
     5581 cgggataata gatcgtttgt ctgagaagta ggagactgtg catcattttc tgggttctcg
     5641 tgatagctat tgtcatatac tctgttgtca aaaactctat tatcgtaagt tctattatca
     5701 ttatttatat gatagtgaat tacaggttgg gatgttttct cattggctcc gggggttggt
     5761 ccgccatctg gcatatcttc gggaggtgta gggaggccat cttggatagg atgcgaatct
     5821 tttatatctt ctaaaatatc attaaggact tttctcaaca gctcaggaaa cacggctact
     5881 gtatttaagt ctcttgacag tttacctata ccaaatcctt tactatcaat agtattttta
     5941 cgttgattgc gcaaactatc aaaggcaagt tttgcctgtg cgtcaactaa atcatctagt
     6001 ttgagagatg aaacagtttc attacttaac tttaattcag caagaatatt attcttgata
     6061 aatgatgata cttcagtatt gaggagtttt tgtcccaatg ataaagcttc accagatgtg
     6121 tgttgttgtg ctaatagtgc aagtctgtgc tcaatgtttt gtcgtaattg atggagtttc
     6181 ttattctttg ccgaccatgg tccaaaggca gaatcttggg ataatttatc ttttataatt
     6241 agttccgaat actgtgcgac aattttttga agtccctttt cgttttcaaa attaagacca
     6301 ttttgatgta ctaaggagta caaaacattt gaaaattcga gaatctcgct tttggcatac
     6361 gcattcattg agttggaata aatgcttctt aacacatctt ctttccctga ggaagaaaat
     6421 aaaaaatcaa gacatttctg attaaaagac gttgcaaagt tttctttttc agacactgat
     6481 gctggtgttt ttagagaagt ttgcgaacta tggatatcgg atattttgct ttttaactcg
     6541 ctgtattctg ttgatgatgg tgaagttgcc ttatataaga atgttggcgc ttgagtatta
     6601 tttacattat gcatggcgca cctcagaaat ggagaaaaag tttatctgta tctgtacatg
     6661 agcttattgt actactcaaa acctttacta aattatcaaa aatactattg gcattactgt
     6721 atttttgaac taaagtttga agattatttt tcattgtttc atcttcggca gagaatccgg
     6781 cattccatgc ctgatatttt gcattatcta gcacaacctc gccatttcca cctagattat
     6841 ctaagctttt taacatattg tctattgggg tcatgtttat actgacaaca tatcccccgt
     6901 ttttttgaga taccttgccg attgttccac ctaattctgt aagccattta tttgcttgtt
     6961 cctgactaac agtattattt gctggatata gcggtttatc tttatatttt tccttgagtt
     7021 cttccaatgc ctttttaagc gagttgactt gtaatttcac ggagtttccg tcgttacctc
     7081 cgggagagat ccagccggca agactggaaa gaacagcgct aaaatcttga tacatttgag
     7141 tatatgaact aacggcatgt tcatatactt tcagatactg ttcattaata tcattgatgg
     7201 agtttgcaat tttagcccac agttctctat gagatatcat ttgatctcca tcaagctcgg
     7261 cttctttcgg agctgaatgt aataattctc ttgcgtcttt attaattgga tattcgtgcc
     7321 tggaaagaat atctaatagt tgagcggatt tatttacatc catgctaatc tgagatgaat
     7381 gtaatgctat ttcttctagc gatgttttag tcaacgtttt ttgtgaaagc tctttcttta
     7441 atgcctgatt tgttgttctg atattatgaa gggtgtcgtt gagcatagta agggaactta
     7501 caggatgaga actggtcgtt gtttttatat cagaattaac tgtttcggtt gatgaaccgt
     7561 tggtattgtt tggactgaat gatgaggtgg aaatactatt agtcagagtt gttatattca
     7621 tggttatttc cttacaggaa gagccatata ttattatggc tcttggatat attaagctcg
     7681 aatgttacca gcaatctgac tggctgtcga attctttgat tggttgatgc tgtcaattat
     7741 attcaataat ttttgtatta attgatttgt cgcttgggat gcttctttag atacttggga
     7801 tgcttcctct gcttgtttac tgctggcctg actgattagt tgttcttctt cttcaagagc
     7861 agatgcataa cgccctcctg atgtggatat attaccagca acggctgatg tttccattgt
     7921 tgcacggcca atgttttgtt tttgctgcgc agaaagggta ttgagctcgt aagttcttct
     7981 ctgggtgtca attttatcct gcaaagaaat atcgggagaa ctaagagaag ttttatgttc
     8041 agttgacagg gatatattat ctggcgccag tttattttta cctaaaaatt ttgtgctaga
     8101 gttagtttgt ggtgaggtga tatttgtatc tatttgttta tttaacccta atttagaacc
     8161 tgcaagctct ttttcaagag attgagcagt ggcaaggttc tttcttaagg ctcctttttg
     8221 gtcgctaatc cctgaatgcg tttttttggc accgataccc gttataccta cttgtgtgac
     8281 tgctccagta atgcttgatg atagggctgc caggccttgc cgaacaatgt tctctgcagc
     8341 tgattttgta gcatcgaacg caatcaatga caattgagag cccaatttag tttctgcagt
     8401 gcggagagca gaaagtagaa cggctacact aataattaaa gaaacagcat tagaagaaag
     8461 actggaaata tccagagtgt tctctggagt aagggtgtga ctcatctctg aaagatcttt
     8521 catctttttg tcagtaaggc gagcaatgat ttctgaatca tgctgaacct gctctgataa
     8581 ctttaaaagt tgatcatcat ttaatgtggt tgttaatacg ggatttttac cgacattaag
     8641 tggaatatgc gctgcaatct gctgataatt ttgtgatttt tgtgtttcgg aagaactttg
     8701 agtttgtttt gtggatatat ctgtatataa aatctgggtt ggttttgtgt tttgaatttc
     8761 cataacattc tccttatttg tatcaagcag tagtttgttg caaaattgct tttgcaacat
     8821 cagttctatt agcctgagag ttggacattg aggctaatag atctgcaatt acttcctgca
     8881 attggccgaa tttttctatt gcttcactga tatattttga cagttgttca acttgatatt
     8941 tcgataatgt caggtctgct agatttgtcg acgcgctgtt ctggaaaaca gcagaagcga
     9001 cacttcctcc cgcttgtgtg gcagagttaa cactttctcc taataaaact gcttggttta
     9061 aatgggtgga aataatttgt ttggatatta cttcatcacc cgctgcacca agaaatttat
     9121 ttaatctggc aacagcatta gtgattaaat cgtccagttg agaagaaaaa ttcttgagaa
     9181 actttggtat aaggtctgtg agggttttac ctattatttt gccaatattt tctgcaagtt
     9241 ttgctgctgc ctgtttacca acagtggcta cgagaacgac tgctgcaact aggacaagag
     9301 cgcctgcgat tgcccccaga atagagccaa tcattttggc ttttttcgag tcgacgccca
     9361 agccttcgag catttttgta aatgcatctg aaaggagttt gattaagggt tcaatgactg
     9421 ctttcatgat cggattcagg gcttgttcca tgaaggaatt gccggtcgct gcttgtacta
     9481 tagcatccgt aaccataaga gctaaaccaa cagctgccag tgctagagag gctcctccag
     9541 aaaaagctgc tgcaacaaca ctaacgatag ttaaaagtgc cccaagtatt ttcccaacac
     9601 aacccattac tctgttgagt tcttctgctt tacgtacttc agcagcatac tcatcagatt
     9661 ttctctccat ttcagttttt cttgattctt ggagagactg gaatagagcc agatcatttt
     9721 ttaaagattc ttcattattt tttccaacta gttgaataaa ggttgccatc aattgagtaa
     9781 cactggcaag tccggttaat gatttctgct gggttgatag ctgttcagca gatgctgtgt
     9841 ttgaaaatgc agaaaaagag tctatttctt tttcgagttg cattgattta tctgtaagtt
     9901 tgctatgaat tgacagggtt ttctgctcaa tcaatgtcct gtctttaact gctgcgtctt
     9961 ttttgatagt gagttgtatt tcttcccggc ttaatttttt cttttctggt gactctgggt
    10021 cgagttcgga taatcttgtt tgaatttggt taattttatt ttctaggtct tttattttag
    10081 aatctgcgtt ttttagttta ttaatttgtt tttcatagtc tctggttagt ccttcagttt
    10141 cagatagaag agtgttaatt ttatcggaga attctaggtt tttttgctgt cttgcctgtt
    10201 gctgggactt ccaagcagta attttatttg ttaatgcagt taaagatttt tcaccgagta
    10261 tttgaataag gtttccaatt aaaagcgtta gttgggaact tgcatttaat gactttggtg
    10321 ctttaagttc agggattaat atatttgaag ttgagtgtgc attagttgta ttaatatttt
    10381 ggttagcagt aagatcagca attgtaagag aaaataattt gttagctgca tcatttgcag
    10441 cttggatagt attgtctcca agctcagtgg aagccaatat tttggcaaga ggaaaaccag
    10501 tggttgtggt gcttacatta tgcataataa ttactccttg atatcctgaa ttgcgtccaa
    10561 gtatgattgt gcttttattt ttaatttttc atcattgctg tgttgaatta cgagttcgaa
    10621 gcactcttta gcttttaagg gggctttcaa ccgaagctgg cattgtccag tatggaatac
    10681 tggtgtatag tcattttttc ctaatgcaaa agcgacagca taaaggtctg ctgcttgttg
    10741 gaactgttct tttatctgat aaatagctgc gagtcccata atgtagtcta cattgtaaaa
    10801 gtcgtatata cataaaaacc tgaagaaaac ttcagcttcc tctattcttc ctttgttgta
    10861 aaagtcataa gcatatgaat aaatgtcatc catcatatca tcaggaattg cattaatatc
    10921 tttcagtgta gcgccagagt taattgcatc aattactgca gtagagatgc tttcattttc
    10981 ggtgatattt aaagacataa ggtctccttt ttgtctgctt tactttaata agtataagat
    11041 ttaatataaa agatttaatt tgtattgctt tgacggtata cagcctttag aacctctgcg
    11101 cttatttcta tttgtaaaac ttttgttgat tcattaaata ttttatcgta gacggaactg
    11161 gatactagat ttgtaagatc cttcatttgt gtgttaaaca aatccatgtc actgctatag
    11221 cgttttaaaa tctttgctct gaggccagat gaggcaatct cgtttagtgc tgtatgactg
    11281 atagatgttt gtgctgattt ttcatcaagt tttatatccg ggaatttttt ttcaatagct
    11341 gcaaatatgt tttttttgtt atctgaggtc aaaacaactc cattgtgtat ggccatttca
    11401 tctatacttc tgtttatcat tgaagagaca taagtggttc gctcttggct catccaacaa
    11461 aatagttgat tccccgaatt actatctttt attttgtcta tgacgctgtc cctgatttta
    11521 tttatattca ccgggcatcc aaaattcttc tgaggaaagc gttgttttaa gttaaaaaca
    11581 gataatattt tctttacaag cttattgcga cttaacgaat caaatgatgg gcgaggaatc
    11641 gcgaattcaa cctgtggaag tattttgttt agaatttgca tgataccccc tatatgttag
    11701 ttcacttctg aagtgatgtt tgcaatggtt agtttaatac aaaataaaaa ttcatacaaa
    11761 gattcaatga gttcatcagg tgaaagatat tttttgtagt gtttcttggt caaaacacat
    11821 tgatgagtat cagggagaag acagatagaa gaaccataat ctctactcaa agagtagtta
    11881 aggatgagta tgtgttctgg attaataata ttttctgtaa aaagttcatg ttcattaaga
    11941 agtccggtca aaaagatgta ttcatcatta tctgttatac aaacaggtat ttgttcttcg
    12001 agcaatataa aggcttgatt tttatcgtta aaatcaagct ttgcacctgt tatttcataa
    12061 agtttatcat atagtttgcg acacatttct atggccttct atatattaga atgagagtta
    12121 ttcaataaaa gcttattgat ctgtaataag tcgtgaatgt aaagtttggt tccaagatct
    12181 ggcgatttaa gagaattgta ataatcaata atttttattg atttttcgat gatttcttct
    12241 tttttatctg ttgcttcggt ttttaattcg tttaatacac gagtcatatt gctgggtgct
    12301 gattttacta agtatgagtg gcaagcgttg atgaccgcat caaagtcttc tgaagataca
    12361 ttttcagtag gatgtttgta aagtgaaatt gataaaggct tgaatgtttt tggtgtgtgg
    12421 ataaggttaa ttttattgaa acttttaaga tagctgcgtc gtaattgagt caatctggaa
    12481 agatcaaaat cagattcaat atacttcatt atttcgttat aagactgatt gagactttca
    12541 atgcgttgcc taactttatt tgcatacgcg tatacatcgt taggtgtagc aaccaattta
    12601 acgtcaggga gtggaaagta tatttcagaa tttcctgctt taagaacatt aagtgccata
    12661 ccagcacagt tttcagattt actgattaat ttataatttg ccaattgtct gaaagcatct
    12721 gctttctttt tgcatataaa ttttgacatg tttgtttcat caaggccaaa aaggttatgg
    12781 ctgattttac catcctttgt tttattatca cctgataatg gaatgtatac tttatcagca
    12841 ctgacccccc aataaccata ggggtctttt ttttgattag ctctgggaaa aaatattttt
    12901 ttgttgttat tttgatcttg ttttgaattt aataagtttc taacctcttg gcctgcatca
    12961 agtctttcgg ctgtttggtc tgaaatcata tttagtttgt cttgcttata ggtatcgaca
    13021 ctgtaacctg agcgtttttc aaaaaggtag tttatttctg tatcttttgt aacattcccg
    13081 ccgggatacc agctgagata ggtatgagtt ggttcctgta gtaaattgtt ttttatactg
    13141 atactagtat gccctaaatg ggttatgcct ctatttcccc agcaacgaaa aactaaatgt
    13201 ctttcaagat ttttggctgt accgatgcca tgaaaacgct ctgcggtatt caacattaat
    13261 ttgatgtctc tacctgtgaa agagtgttga attgagtttt tagctgcttc ataggcggca
    13321 aacgcacttt ctgcagattt ttcgctagct aatgtattga ggaaaagcaa taaggatttt
    13381 tgattctcta gtcgataatt ttttagttgt tcatttaata tctgtgaatt tatctttgca
    13441 ttaatagtgc ttaatgttct gaaaaagaga tcctttaggt tgaatttctg tgctatcaat
    13501 ataggtccat gctctgtatc ttcaacgcgt ataggccctt ttgtattgct tgcgtcaatg
    13561 aaattgctaa ttttgaggat catactttat taactctcca ttacttggtg attgtgggga
    13621 tgtaaaatgt atttggtata taatatattc aattaataaa tttagaaact tgagcctgtt
    13681 aacataatca aattttcttt tgctgtacat aatatgtacc tcgtgagcat atgtagtgct
    13741 cgtttcatca tgaaatccca caagataaag tgcctgatgt atcaggctcg gagtgttata
    13801 gaaaaagaga gaaccctgtt gaataagtat cttttttgtt gtaaaacgag gtacaaaact
    13861 gcaatcaatg ccccatttat gactgaggat aattaaatgc acataactaa tttgggattg
    13921 catcaggttt catttcaaag cggagattcc tataaaggcg cagaagaaac cggaaagcac
    13981 aaaggtgtaa gcgtgatttc atatcaaaga gttaaaaatg gagaaagaaa taaggggatc
    14041 gaagcgctta atcgattata tttacagaac caaacaagct taacaggaaa aagccttttg
    14101 tttgcgcgtg acagagctga agttttttgc gaggcaataa agttggcagg tggtgatacg
    14161 tcaaaaatta aagccatgat ggaacgatta gatacctata aacttggtga agttaataaa
    14221 agacatatta atgagcttaa taaagtaata agtgaagaga taagagcaca gctaggcatt
    14281 aaaaataaaa aggaattgca aacgaaaata aaacaaatat ttactgatta tttaaataat
    14341 aagaattggg gaccggtcga caagaatatt agtcatcatg ggaaaaatta tggttttcaa
    14401 ttaactcctg cctctcatat gaagataggg aataaaaata tatttgtcaa agagtataat
    14461 ggaaaaggaa tttgttgtgc ttctacgaga gagagtgatc atatcgcgaa tatgtggctt
    14521 tcaaaagtgg tggatgatga ggggaaggaa attttttcgg gtatcagaca tggagttata
    14581 tcagcctatg gattgaaaaa aaactcatct gaaagagctg ttgctgctcg taataaagct
    14641 gaggagttag taagcgcagc attatatagc agaccagagt tattatcaca ggctttgtct
    14701 ggtaaaacag tagatttaaa gattgtttcg acttctcttc tgacgccgac cagtttaacc
    14761 gggggagagg agagtatgtt aaaagatcag gtgaacgcat taaaagggtt aaactctaaa
    14821 agggggaagc caacgaaatt attaattcgg aatagtgatg gtcttctgaa agaagtaagc
    14881 gttaatctaa aagtggtgac atttaacttt ggtgtaaatg aattagcgct aaaaatgggc
    14941 ttaggctgga ggaatgttga caagcttaat gatgaatcaa tatgttcttt gttgggggat
    15001 aatttcctca aaaatggtgt gattggaggt tgggctgcag aagcaataga aaaaaatcca
    15061 ccatgtaaga atgatgtgat atatctggct aaccagataa aagagattgt aaccaaaaag
    15121 ttacaaaaaa atgataatgg agagccgtat aaattgtcac aaagaatgac tctattggct
    15181 tatactatag gtgctgtacc ttgctggaat tgtaagagtg ggaaggacag aacaggtatg
    15241 caggatgctg aaattaaaag agaaataata agaaaacatg aaacaggtca gttttctcaa
    15301 ttaaatagta aattatcctc agaagagaaa agattatttt ctactattct aatgaatagt
    15361 ggtaatatgg aaatccaaga gatgaatact ggtgtgcccg gaaataaagt catgaaaaaa
    15421 ttgccgctat cttcgttaga gctatcttat tctgaaagaa taggggaccc aaaaatatgg
    15481 aatatggtga aagggtattc gtcatttgta taagaggaat atatggaaga tttagcagat
    15541 gttatttgcc gagccttggg tatcccttta attgatatcg atgatcaagc aatcatgctt
    15601 gatgatgatg tgctaatata tattgaaaaa gaaggagatt caataaattt attatgccct
    15661 ttttgtgcat tgcctgaaaa tataaatgat cttatatatg cgttgagcct aaattactca
    15721 gaaaagatat gcttagctac tgatgatgaa ggcggaaatc taattgcgcg attagatctt
    15781 accgggataa atgaattcga ggatgtatat gttaatacag aatattatat ttcgcgtgtg
    15841 cgttggctga aggatgaatt tgcgcgaaga atgaaggggt attaatgtcc cgtttcgtat
    15901 ttattttgct gtgttttatc ccatatttag ggagagctga ttgttgggat aaggctggtg
    15961 aaaggtacaa tattccatca agtttgttaa aagcgattgc ggaaaaagaa tccggattta
    16021 ataaatctgc agtcaatgtt aataacaatg gaagtaaaga ttatggtata atgcaaataa
    16081 atgactttca ttctaaaaga cttagagaaa tgggatattc tgaggaaatg ctaattagtc
    16141 atccatgtct ttctgtacat tatgcagcaa agttattaaa tgagtttatg atgatgtatg
    16201 gaagagggtg ggaggctgtt ggagcctata atgcaggaac gtctccgaaa aagaaaaaag
    16261 aaagattaaa gtacgcagag gatatctata gacgctattt gcggatagca gcagagagca
    16321 agcagaataa tcgaaggata taagaggatt tatgtctgag gcaaagaact caaatcttgc
    16381 accattcagg ttattagtga aactcacaaa cggtgtcggg gatgagtttc cgttatatta
    16441 tgggaataac ttaattgttc tcggacggac gatagaaaca cttgagtttg gaaacgacaa
    16501 tttcccagaa aatattattc ctgtaacaga tagtaaatcg gatggtataa tttatttgac
    16561 cataagcaaa gataatatct gtcaatttag taatgaaaaa ggtgagcaaa ttgatatcaa
    16621 ttcacaattt aattcatttg aatatgacgg gatttctttt caccttaaaa atatgaggga
    16681 ggataaatcg agaggccata tactaaacgg aatgtataag aaccactcgg tgtttttttt
    16741 ctttgctgtt attgttgtgt taattataat tttttccctc tctttaaaga aagatgaagt
    16801 aaaagaaatt gctgaaatta tagatgataa gagatatgga atagtcaaca ctggacagtg
    16861 caattatatt cttgccgaaa cacaaaatga tgcggtatgg gcgtctgtag ccttaaataa
    16921 aaccggattt accaaatgca gatatattct ggtgtcaaac aaagaaataa atagaattca
    16981 aaaatatatc aatcaacgat tcccatttat caatttgtat gtgttgaatt tagtgagtga
    17041 taaagcggaa ttactggtgt ttttaagtaa agaaagaaat agttcaaaag ataccgaact
    17101 tgataaattg aaaaatgctc tgattgttga atttccgtat atcaaaaata ttaaattcaa
    17161 ctatcttagc gatcataatg caagggggga tgctaaaggt attttcacaa aggtaaatgt
    17221 ccagtataaa gagatttgtg aaaataataa agttacatat tctgtcagag aagaactgac
    17281 agatgaaaag cttgagctca ttaatagact gatttctgaa cataaaaata tatatggaga
    17341 tcagtatatt gagttttctg tacttttgat agatgatgat tttaaaggta aatcatatct
    17401 taacagcaaa gacagttatg taatgttgaa tgataaacac tggttttttt tagataaaaa
    17461 taagtgagga taaaatgagt gttacagtac cgaatgatga ttggacattg agttcattat
    17521 ctgaaacttt tgatgatgga actcaaacat tacaaggtga actaacattg gcactagata
    17581 aattagctaa aaatccttcg aatccacagt tgctggctga ataccaaagt aaattatctg
    17641 aatatacatt atataggaac gcgcaatcca atacagtgaa agtgattaag gatgttgatg
    17701 ctgcaattat tcaaaacttc agataatagg gagcattcat gaattacatt tatccagtca
    17761 atcaggttga tattatcaaa gccagtgatt ttcaatctca agagatatca agtctggaag
    17821 acgtcgtgtc ggctaaatat agtgatatta agatggatac agatattcaa gtatcacaaa
    17881 taatggagat ggtaagcaat ccagaatcat taaacccaga atctttggcc aagttacaga
    17941 cgacgctctc aaattattca ataggagtat cattagctgg cacgttagca agaaaaacag
    18001 tttcggctgt tgaaacttta ttaaagtctt aatttatatg attaggtata aaggttttat
    18061 tttattcttg ttgctgatgt tgattggatg tgagcaacgt gaagagttaa tttctaattt
    18121 atctcaaaga caggcaaatg aaataatatc tgtgctagaa cgccataata ttactgctag
    18181 aaaagttgat ggaggtaaac aggggatctc ggtacaagtc gaaaagggga catttgcatc
    18241 ggcagttgat ttgatgcgca tgtacgattt gccaaatccg gagagagttg atatctcaca
    18301 aatgtttcct acagattcat tagtgtcttc tccaagagct gaaaaggccc gtttatatag
    18361 tgctattgag caacggctgg aacagtcttt agtttctatt ggtggtgtta tttcggcaaa
    18421 aatacatgtt agctatgatc ttgaagaaaa aaatatatct tcaaaaccga tgcatatatc
    18481 agtaatcgct atatatgact caccgaaaga gtctgaacta ttagttagta atattaagcg
    18541 atttttgaaa aacacctttt ctgatgttaa gtatgaaaat atatctgtca tattaactcc
    18601 gaaagaagaa tatgtttata caaatgtaca acctgttaag gaagttaaat cggaattttt
    18661 aacaaatgaa gtaatatatt tatttctcgg gatggctgta ctagttgtca ttcttttggt
    18721 atgggcattc aaaacagggt ggttcaagag aaacaaaata tgataagaat ggatggaatt
    18781 tataaaaaat atctttcaat aatttttgat ccagcgttct atataaatag aaatcggttg
    18841 aatttgcctt ctgaactgtt agaaaatggc gtaatcagaa gtgagattaa taatctcata
    18901 attaataaat atgatctaaa ttgcgatatt gaacctttaa gcggggtaac cgctatgttt
    18961 gttgccaact ggaatttact tccagctgtt gcttatttta tcggtagtca agagtctcgg
    19021 ttaattaatc atagtgagat ggttattagt tattatgggg gcaaaatcag taagcaaggg
    19081 gaggccgcta ttcgaagtgg tttttggcat cttattgcgt ggaaggaaaa tatatctgtt
    19141 ggtatatatg aaagaataaa cttattattt aatccaattg ctttggaggg aaattatacg
    19201 cctgtagaaa gaaatttgtc ccgattaaat gaaggtatgc aatatgcaaa aaggcacttt
    19261 accggtatcc agacatcatg cctatgatgg tgttgttata aagagaattg aaaaggaatt
    19321 atgtaaaacc ataaaagata gagacactga gagtaagaag aaagcaattt gtgttataaa
    19381 ggaggctacg aaaaaagctg aatcactgcg tatagatgct gtatgtgatg gttatcaaat
    19441 tggtatacag actgcttttg agcatattat tgactatatt tgtgaatgga agttaaagca
    19501 aaatgaaaat agaagaaata ttgaggacta tataactagt ttgctttcag aaaatttaca
    19561 tgatgagagg atcattagca ctttattgga gcagtggctt agttcgttgc gtaatacagt
    19621 aacagaatta aaagtggttt tgcccaagtg taatttagca ctgaggaaaa agttagaact
    19681 tgatctccat aaatatcgct cagatgtcaa gattatttta aaatattctg aagggaataa
    19741 ttatatattc tgtagtggta atcaagtagt ggagtttagt cctcaggatg taatatcagg
    19801 tgttaaaata gaacttgcgg aaaaattaac aaaaaatgat aaaaaatatt tcaaagagtt
    19861 agcacataag aaactgagac aaattgcaga agacttgtta aaagagaatc ctgttaatga
    19921 ttaatcaaat aaatgcaagc aatgctctgc agcaaagatt aaatagtgaa gaagttgtta
    19981 atttaaatga gagattatca tcttcgcaat catttgatga ggatataatc tatgaaataa
    20041 tgcaatattt ctcacagagt gaattaaatt ccatagataa tgatgaactg cacaataaga
    20101 ttgaacaatt attcaattca cgttttcctt atcttacagc tgcacaaaaa agttcacttc
    20161 ttaataaact catagatgca aatcaatatg tcgatttgca tgagggattt tatgcatcat
    20221 tgtccatata taacaatatt gatttctata ttaagactac aaccttcgat tcattaatta
    20281 gtgtctttga agcagggaga gaggcagatg attcgacatg gtagtaataa gttgaaaata
    20341 tttattttaa gtatattgct attaacactg agtgggtgtg ctttaaagtc atcatctaat
    20401 tctgaaaaag aatggcatat tgttcctgta agtaaggatt atttttctat tccaaatgat
    20461 ttattatggt cgtttaatac aaccaataaa agtataaatg tttactctaa atgtattagt
    20521 ggtaaggcgg tttatagttt taatgcaggt aaattcatgg gcaactttaa tgttaaggaa
    20581 gtagatgggt gcttcatgga tgcacaaaag atagctatag ataaactatt ttctatgctg
    20641 aaagacgggg ttgttttaaa aggtaataag ataaatgata ccatccttat agagaaggat
    20701 ggggaagtta aattaaaatt aattcgaggg atataattgt attgtgagta aatataaagg
    20761 tctaaataca agtaatatgt tttacattta ctctagtgga catgaaccag ttaacgttga
    20821 gcttgtaaaa gataaagaac gtaacataat tgagctggct ccagcatgga agggtttttt
    20881 tttgtccgaa atcaaaatat aaaattcagt gataatgtga actatcatta tcgctttaac
    20941 attaatagtt gtgcaaaatt tcttgctttt tgggactatt tcagtggtgc tctagtagag
    21001 catagccatg ctgaaaaatg tattcatttc tatcatgaga atgatcttcg ggatagttgt
    21061 aataccgaat cgatgcttga taagttaatg ttaagattca tcttttcatc tgaccagaat
    21121 gtaagtaatg cactggctat gatacgaatg actgagtcat atcatttggt tctttatctt
    21181 ctgagaacta ttgagaaaga aaaggaggta agaataaaga gtctgacaga gcattatggt
    21241 gtgtcggagg cttattttag atctttatgt agaaaagctt taggggcaaa agtcaaagaa
    21301 caactgaaca cttggcggtt agtaaatggt ttattagatg tatttttgca caatcaaaca
    21361 atcacttcag ctgccatgaa taatgggtat gcatcaacat cccatttttc aaatgagata
    21421 aaaacacgac taggcttttc tgcaagagaa ttatcgaata ttactttttt agtgaaaaaa
    21481 ataaatgaaa aaatttaata ttaaatcttt gactctcttg attgtattgt taccattgat
    21541 agttaatgct aataatattg actctcattt attagaacaa aatgacatag cgaaatatgt
    21601 tgctcaaagt gatacggtag gatctttttt tgaacgtttt tctgcgttat taaattatcc
    21661 tattgttgtt agcaaacaag ctgcaaaaaa gagaatatct ggggagtttg atttatctaa
    21721 tccagaagaa atgcttgaaa aactgacatt acttgtggga ctcatatggt ataaagatgg
    21781 aaatgcatta tacatatatg actctggaga attaataagc aaagttattt tgctggagaa
    21841 tatatcactt aattatctta ttcagtatct gaaggatgct aacctttatg atcacagata
    21901 tcctataaga ggaaacatta gtgataaaac attctatata tctggtcctc cggcattggt
    21961 tgaactggta gctaatacag caactttgct tgataagcag gtttcttcta ttgggacaga
    22021 taaagttaat tttggggtta taaaattaaa aaatacattt gttagtgaca gaacttataa
    22081 tatgagaggg gaggatattg tcatcccagg ggtggctact gttgtagaac gtttattaaa
    22141 taatggtaag gctctgagta acagacaagc ccaaaatgat ccgatgccac cctttaatat
    22201 tacacagaaa gtatctgagg atagtaatga cttttcattt agttcagtta ccaatagttc
    22261 tatattagag gatgtatcac ttattgcata tccagaaacc aattctatat tagtcaaagg
    22321 taatgatcag caaatacaga ttattcgcga tattatcact cagttagata tagccaaacg
    22381 acatatagaa ttatcattat ggataatcga tattgataaa tctgaattaa ataaccttgg
    22441 tgttaattgg caaggaacgg cttcatttgg tgattcgttt ggtgcgtcat ttaatatgag
    22501 ctcatcagca tcgataagca ctttggacgg caataagttt attgcttcag ttatggcatt
    22561 aaatcagaaa aagaaagcaa atgtagtttc aaggccggtg attttgacac aagagaatat
    22621 tccggctata tttgataata acaggacttt ttatgtgtca ttagttggtg agcgcaattc
    22681 ttcgttggag catgttacgt atggaacatt gataaatgtt atacctcgct tttcatctcg
    22741 gggacaaatt gagatgtctt taacgataga ggatggaaca ggaaatagtc agtcaaatta
    22801 caattataat aatgaaaata catctgtcct tccagaagtt ggaaggacca aaatcagtac
    22861 aatagcgaga gttccacaag gcaaaagttt gttgataggt ggttatacgc atgaaacgaa
    22921 ctctaacgaa ataatatcta ttccttttct ctcctctatt cctgtaatcg gtaatgtgtt
    22981 caagtataaa acaagtaata tcagtaatat tgtaagggtt tttctgattc aaccccgtga
    23041 gataaaggag tcaagttatt ataatactgc agaatataaa agcctcatat ctgaaagaga
    23101 aatacaaaag actacacaaa taatcccatc tgaaactact ttgctggaag acgaaaaatc
    23161 attggtttca tacttaaatt actaactata aagtaggtga tgtatgcttg atgttaaaaa
    23221 tacaggagtt tttagctctg cattcattga taagttgaat gcaatgacaa attcagatga
    23281 tggagatgag actgctgatg cagagcttga ttctggcttg gctaatagca agtatattga
    23341 ctcatctgat gagatggctt ccgctctttc gtcatttata aacagaagag accttgagaa
    23401 actgaaagga acaaatagtg atagtcagga acgtatttta gatggggaag aagatgaaat
    23461 taatcacaag atttttgatt taaagagaac gttaaaagat aaccttcctc tagatcggga
    23521 ttttatagac agactaaaga gatattttaa agatccaagt gatcaagtct tagcattaag
    23581 ggaacttttg aatgaaaaag atcttactgc tgaacaagtc gaattattaa ctaaaattat
    23641 taatgagata atatcaggta gtgaaaaaag tgttaatgct ggaataaatt cagctataca
    23701 ggctaaatta tttggcaaca aaatgaaact tgaaccacag cttttgcgtg catgttatcg
    23761 tggttttatc atggggaaca tatcaacaac agatcagtat atagaatggc ttggtaattt
    23821 tggttttaat cacagacata caattgtgaa ttttgtagag cagtcactga ttgtagacat
    23881 ggattctgag aaaccgagct gtaatgctta tgagtttggt tttgtgttat ctaaattaat
    23941 tgcaattaag atgattagaa cttcagacgt aatttttatg aagaaactgg aatcctcaag
    24001 cttgctaaaa gatggcagtt taagtgcaga gcagctattg ctaactttat tatatatttt
    24061 tcaatatcca agtgaaagtg agcaaattct tacttctgtt atagaagtat cacgagccag
    24121 tcatgaggat tctgtagtgt atcaaacata tctatcttct gttaatgaaa gtcctcatga
    24181 tatatttaaa agtgaaagtg aaagagaaat tgcgatcaat attctacgag agcttgtcac
    24241 aagtgcatac aagaaagagc tttctagata acaggagata aaagtgatcc agtcttttct
    24301 taagcaagta agtactaagc ctgaattaat tatattagtg cttatggtta tgatcattgc
    24361 tatgttgatc ataccattac ctacatacct tgttgatttt ttaatagggc ttaatattgt
    24421 acttgctatt ctcgttttta tgggatcatt ttatattgaa agaatattga gtttttctac
    24481 attcccttcg gttttgttga taacgacatt atttcgtctc gctttatcaa taagtactag
    24541 tcgactaatt ttagttgatg cggatgcggg taaaattatt actacatttg gccagtttgt
    24601 cattggtgat agtctggctg taggttttgt tattttttca atagtaactg ttgtgcagtt
    24661 cattgttata acaaaaggat ctgaaagggt tgcggaggta gctgctcgct tttctcttga
    24721 cggtatgccg gggaaacaaa tgagcattga tgcggattta aaggctggaa ttattgatgc
    24781 cgcaggagct aaagaaagac gcagtattct ggaacgtgag agtcaattgt atggttcatt
    24841 tgatggagca atgaagttca ttaaaggtga tgcaattgct ggcatcatta ttatttttgt
    24901 caatttaata ggaggtattt ctgttggtat gagtcaacat ggaatgtccc tatctggtgc
    24961 attatctact tataccatac taactattgg tgacggattg gtctctcaga ttcctgctct
    25021 gttaatttct ataagtgcag gatttatcgt tacaagagtt aatggtgata gcgataatat
    25081 gggacgtaac ataatgtccc agatatttgg gaatcctttt gttctcattg ttacatcagc
    25141 tcttgccttg gcgataggga tgttgccagg ttttccgttt tttgtttttt tcctgatagc
    25201 agttactttg acggctttat tctattataa aaaggtcgta gaaaaagaaa aaagtctgtc
    25261 tgagtctgat tctagtggct atactggtac atttgatatt gataatagcc atgactcatc
    25321 tttggcaatg atagaaaatc tggatgctat tagttcagaa accgttcctt taattttatt
    25381 atttgccgaa aataagataa atgcaaatga tatggaaggt cttattgaaa ggataagaag
    25441 tcagttcttt attgattatg gtgtcaggct tccaactatt ttatatagaa caagcaatga
    25501 gcttaaggtc gatgatattg ttttgttaat aaatgaggtg cgtgccgata gttttaatat
    25561 atattttgac aaagtgtgta ttacagatga aaatggagat atagatgctc taggtattcc
    25621 tgttgtttca acttcatata atgaacgtgt tatttcttgg gtagacgttt catatacaga
    25681 aaatctaact aatattgatg ctaaaattaa aagtgctcaa gatgaatttt atcaccagtt
    25741 gtcacaagct ttattaaaca acataaatga gatttttggt atacaagaaa caaaaaatat
    25801 gttagatcag tttgaaaatc ggtatcctga tctattaaag gaagtcttcc gacatgtgac
    25861 tatacagaga atttctgagg tattacaaag attgcttgga gaaaatattt ctgttcgcaa
    25921 tttaaaactt attatggagt ctttggcgct ttgggctccc agagaaaaag atgtcataac
    25981 attagttgaa catgtccgtg catcactttc taggtatatt tgtagtaaaa tagctgtttc
    26041 tggtgagatt aaagttgtga tgctttccgg atatattgag gatgcaataa gaaaagggat
    26101 aaggcaaacc tctggtggct ctttcttgaa tatggatata gaggtttcgg atgaggtaat
    26161 ggaaacttta gcacatgctt tgagagaatt gagaaatgca aaaaaaaatt tcgttctttt
    26221 ggtatcagta gatatacgta ggtttgttaa aagacttata gataacagat ttaagagtat
    26281 actcgttata tcgtatgctg agattgatga agcatatacc attaatgtat taaagactat
    26341 ttagtgaggt ttaaatatga gtaacattaa tttagttcaa ttagttagag atagtctttt
    26401 cacgattggt tgtcctccat caattattac agatttagac agccattcag cgattaccat
    26461 ttctttggat agtatgcctg ctattaatat agctcttgtc aatgagcagg ttatgttatg
    26521 ggctaatttt gatgcaccaa gtgatgtaaa acttcagtca tctgcttata acatattaaa
    26581 tttaatgtta atgaacttta gttatagcat taatgagctg gtagagcttc atcgttctga
    26641 tgagtactta cagttaagag ttgttattaa agatgactac gttcacgatg ggattgtttt
    26701 tgcagagatt ctgcatgagt tttatcagag gatggaaatc ttaaatgggg tcttataatc
    26761 aatgagctat acaaaattgc tcactcaatt atcttttcct aatagaatct cggggccaat
    26821 cttggaaaca agtcttagcg atgtttcgat tggtgagatt tgtaacattc aggctggaat
    26881 tgaaagtaat gaaattgttg caagagctca ggttgtagga tttcatgatg aaaaaacaat
    26941 attaagcttg attggaaatt ctcgtggact ttcacggcaa acgttgatta agcccactgc
    27001 ccagtttctt catacgcaag tcggccgtgg attattggga gcagtagtca atcctttagg
    27061 ggaggttact gataagtttg ctgttacaga taacagtgaa attctttatc gacctgtaga
    27121 taatgctcct ccgctatata gtgaaagggc tgcaattgag aagccttttt taacaggtat
    27181 taaggttatt gattctttac tcacgtgtgg tgaaggacag cgaatgggga tttttgcgtc
    27241 agctggttgt ggcaaaactt ttctcatgaa tatgctcatt gaacatagtg gtgctgatat
    27301 atatgttatt gggttaattg gtgagcgagg tcgagaggtt actgaaacgg ttgattattt
    27361 gaaaaactct gagaaaaaaa gcaggtgtgt tttagtatat gcaacttcgg attactcttc
    27421 ggttgatcgt tgtaatgctg catatatagc cactgctata gccgaatttt ttaggactga
    27481 aggacataaa gtagcgcttt ttattgattc attaacaagg tatgccagag cattacgtga
    27541 tgtggcctta gccgctggag aatcacctgc cagaagaggc tatccggttt cggtttttga
    27601 tagcttaccc agacttcttg aaaggccagg aaagttaaag gcaggtggct ctattactgc
    27661 attttacact gttcttttgg aggatgatga ttttgctgat ccattagctg aagaggtaag
    27721 atccatttta gatggacata tatatttgag cagaaatcta gcccaaaaag gacaatttcc
    27781 tgcaattgat tccttaaaaa gtataagcag ggtatttaca caggttgttg atgaaaaaca
    27841 tcgtattatg gccgctgcat ttcgggagct actttctgaa atagaagagc taaggacaat
    27901 tattgacttt ggtgaataca aaccggggga gaatgcctct caggataaaa tctacaacaa
    27961 aatatctgtt gttgagagtt ttctgaagca agattatcga ctcggtttta cttatgagca
    28021 gacaatggag cttattggtg aaacaattag ataaggtttt aaaaatcaaa gataaatatc
    28081 aacggtctgt taaattaatt gaagcacaca tattaacact aaaaaaaaaa ctctttgtat
    28141 cgagatgtgg aggcattaga taaaaggatt atatacttcc tgcaactgga gaatgactta
    28201 gaacctgtag gcgctcagag tgttagtcag cttttcaata caagacgtaa gatcgctata
    28261 gtaaaaaagc atattatcca atatcagtct gaacgaattc ttctaaaggg aagaattgaa
    28321 gaaattcaaa aagatattga tgaggcaaat gcttctaaaa gaaaactgct tcataaagag
    28381 agtaaaatat gtaaacggat tggcttgatt aaaagaaata actttgccaa acagttgata
    28441 cttgatgagt tatcacaaga ggatatgaag tatggcatta gataatataa acctaaattt
    28501 tagtagtgac aaacagatag aaaaatgtga gaaactatct tcaatagata atattgatag
    28561 tctcgttttg aagaagaaga ggaaggttga aattcctgag tactctttaa ttgcatctaa
    28621 ctattttact attgataagc actttgaaca taagcatgat aaaggagaaa tttatagtgg
    28681 cattaaaaat gcgttcgaac ttagaaacga acgagcgaca tattctgata ttccagaatc
    28741 aatggccatt aaagaaaata ttttgatacc agatcaagat atcaaagcaa gggaaaaaat
    28801 aaatatcggc gatatgaggg ggatcttttc atataataag agtggaaatg cagacaagaa
    28861 cttcgaaaga agtcatactt cttctgtaaa ccctgataat ctgctagaat ctgataatag
    28921 aaatggtcaa attggtttaa aaaatcatag cttgtctatt gataagaata ttgctgacat
    28981 catttcttta ctaaatggaa gtgttgctaa atcatttgag ctgcctgtaa tgaataaaaa
    29041 tactgcagac ataaccccat ccatgtcatt gcaagaaaaa tcaatagttg aaaatgataa
    29101 aaatgttttt caaaaaaata gtgaaatgac ttaccacttt aaacagtggg gggctggaca
    29161 ttctgttagt atttcagtgg agtctggttc ttttgttcta aaaccgtcag atcaatttgt
    29221 aggaaataaa cttgacttaa ttttgaaaca agatgctgag ggtaattaca gatttgatag
    29281 cagtcaacat aataagggga ataaaaataa tagtacagga tataatgaac agagtgaaga
    29341 agaatgctaa gaattaaaca ttttgacgct aacgaaaaac tacagatttt atatgcaaag
    29401 caactctgcg agcgtttttc gattcagaca ttcaaaaata aatttacagg cagtgaaagt
    29461 ttagtcactc ttacttccgt gtgtggggat tgggtaattc gtattgatac attatctttt
    29521 ttgaaaaaaa aatacgaggt attttcagga ttttctacac aagaatcttt actgcattta
    29581 tcaaaatgtg tctttataga gtcgtcatct gtattttcga ttccagaact gtctgataag
    29641 attactttcc ggatcacgaa tgaaatccag tatgcaacta ctgggagtca tttatgctgt
    29701 ttttcctctt ctttaggtat tatttatttt gacaagatgc cggtattacg taatcaagtt
    29761 tctcttgact cattgcatca tcttttagag ttttgcttag gttcatctaa tgtaaggctg
    29821 gctactttaa aaagaattcg cactggtgat ataatcatag ttcagaaact ttataattta
    29881 ttattgtgta atcaagttat tattggggat tatattgtga atgataataa tgaggcaaaa
    29941 attaatctgt cagaaagtaa tggtgagtca gaacacacag aagtttcttt ggcattattc
    30001 aattatgatg atatcaatgt aaaagtggac tttattcttt tagaaaaaaa tatgacaatc
    30061 aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt ttcccgatga catagttaaa
    30121 catgtaaata ttaaagtaaa tggttctttg gttgggcatg gggaacttgt ttctattgag
    30181 gatggttatg gtatcgagat tagttcttgg atggtaaagg agtaatctca tgctgagtga
    30241 catgtccctc atcgctacac tttctttttt tacgctatta ccatttttag ttgctgctgg
    30301 tacctgttat ataaagtttt ctattgtttt tgtaatggta cgaaatgcac ttggtttgca
    30361 gcaggttcca tcaaatatga ctctcaatgg tattgcattg attatggctt tgttcgtgat
    30421 gaaacccatt attgaagctg gttatgaaaa ttatctaaat ggccctcaaa aatttgatac
    30481 aatatctgat attgtccgtt tttctgattc tggcttgatg gaatataagc aatacttgaa
    30541 aaagcataca gatcttgagt tagcacgttt tttccaaagg tctgaagaag aaaatgctga
    30601 tttaaagagt gcagagaata atgattattc attgttttcc ctcttgcctg catatgcgct
    30661 aagtgaaatc aaagatgcgt tcaaaatagg tttttattta tatttgccat ttgttgttgt
    30721 tgatcttgtt atttcaagca ttttacttgc acttggtatg atgatgatga gtccaatcac
    30781 aatatcagta ccaataaaac ttgtattatt tgttgcgctt gatgggtggg gtatcctgtc
    30841 taaggccttg attgaacaat atatcaatat tcctgcttag atgatggagg taatgtgtct
    30901 gatatagttt atatgggtaa taaggctctt tatttaatcc ttatcttttc cttatggcca
    30961 gtaggtatag ctacggttat tggattatct attggtttgt tacagacagt gactcaactc
    31021 caagagcaga cacttccttt tggtataaag cttataggtg tctcaatatc tttgctactt
    31081 ctttctggat ggtatggtga ggttttattg tctttttgtc atgaaataat gtttttaatt
    31141 aagagtgggg tttgatggac atttcaagct ggttcgaaag tattcatgtg tttttaatac
    31201 tcctgaacgg cgtttttttt agattggctc cattattttt ctttcttcca tttttaaata
    31261 acggtataat ttctccatct attagaatac ctgtgatttt tcttgttgca tcaggattaa
    31321 ttacttctgg taaggtagac ataggttctt ctgtttttga acatgtttat ttccttatgt
    31381 tcaaggaaat aattgttggc ctccttctct ctttttgctt gtctcttccc ttttggatat
    31441 ttcatgctgt tggtagcatt attgacaacc agcgtggggc aacgcttagt agttcaattg
    31501 atcctgccaa tggtgttgat acgtctgagt tggcaaaatt tttcaatctt ttttctgcag
    31561 ttgtatttct atacagtggt ggtatggtct ttattttaga atccatacaa ttgtcttata
    31621 atatatgccc gttattttct caatgttctt tccgtgtctc aaatatctta acatttctga
    31681 ctttattggc aagtcaggct gttattttag ccagtcctgt tatgatagta ttgttactat
    31741 cagaagtatt acttggtgta ttatcgagat ttgctccgca gatgaatgct ttttccgtat
    31801 cattaactat taaaagttta cttgcaatat ttattatttt catctgttct tctactattt
    31861 acttttctaa agttcaattt ttcctcggtg aacataaatt tttcacaaat ctatttgtta
    31921 gataaaatat tatggcaaat aaaacagaaa agccgacacc taaaaaacta aaggatgccg
    31981 caaaaaaagg acagtcattt aaatttaagg atttaacgac tgttgttatt attctggtag
    32041 ggacatttac tataatatca ttcttttcct taagtgatgt aatgctctta tacagatatg
    32101 taataattaa tgacttcgaa attaatgagg gtaaatactt ttttgcagtg gttattgtct
    32161 tttttaagat aattggcttc ccactttttt tctgtgttct ttcggctgtg ttgccaacat
    32221 tggttcaaac aaagtttgtt cttgcgacta aagctatcaa gattgatttt tcagtattaa
    32281 accctgttaa agggttaaaa aaaatattta gtataaagac aataaaagaa tttttcaaaa
    32341 gcattctgct tcttattatt ctagcattaa caacctattt cttttggatt aatgaccgaa
    32401 aaataatttt ttctcaggtg ttttctagtg ttgatggctt atatcttatt tgggggaggc
    32461 tgtttaagga tataatatta tttttcttgg cattttctat tcttgttatt attcttgact
    32521 ttgtgattga gttcatttta tacatgaaag atatgatgat ggataaacag gagataaaaa
    32581 gagaatatat agagcaagag ggacactttg agacaaagtc gagaaggcgt gagttgcata
    32641 tcgagattct ttcagagcag actaaatctg atatacgtaa ttcaaaatta gtggtaatga
    32701 acccgactca tattgcaatt ggtatttatt ttaatccaga aatagcgcct gcacctttta
    32761 tttctctcat tgaaactaac cagtgtgcct tggctgtcag aaaatatgca aatgaagttg
    32821 gtataccgac tgtgcgtgat gtgaaattag ctagaaaact atataaaaca catacaaaat
    32881 atagttttgt tgattttgaa cacttggatg aagtcctacg tcttattgtt tggcttgagc
    32941 aggttgaaaa cactcattaa taagtaaagg agatgtgtta tatgggagtt aatttctgta
    33001 ataaaatagg tattgatcag tctgaatttg aaatagaatc ttccatcatt aactccattg
    33061 ctaatgaggt attgaaccca atatctttcc ttagcaataa ggatataata aatgttttac
    33121 tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga catttatcgt tgtgctctgg
    33181 agttggtcgt tgaaaaaaca cctgatgatt tataatcatt cataataact cttttgctga
    33241 tataaatatt tttgttgaag atatttatgt agtcacagga tcaattgtag aaaattgtta
    33301 tgtttttgtt aggaactgca ctaaataata tatgataaga cagcaaaaac gactgaccat
    33361 aatccttctt ttattaggag ttgataagag agactattca tcctgtaatg ttaaaactct
    33421 gttatatagt attcgcgatt atgcaaagtc tgtaaatgat catgagatac taaccgagag
    33481 taatcgttta ttatctcatt gcatttcaga tagtaacgga gctttcttta aaagtagtaa
    33541 atacgttccc cttaagtact tacgtaaaag gagaatagca cgaaagatac caaatgatta
    33601 gcactttgct agtaagcttg ctggtaaact atattgaccg ttgggtttgt tttgagactg
    33661 taatttaaat tgtgtaattg cctgtttttg atatgtttac tccaacaacg gagacaggta
    33721 aattatggac gaaaagaagc ttaaagccct tgcggctgaa ctgg
//
LOCUS       DAJTYM010000037        33093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTYM010000037 DAJTYM010000000
VERSION     DAJTYM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(2..94)
                     /locus_tag="OO575_001793"
     tRNA            complement(2..94)
                     /locus_tag="OO575_001793"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO575_001794"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO575_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6664786.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO575_001795"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO575_001795"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6664787.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO575_001796"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO575_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6664788.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO575_001797"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO575_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6664789.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO575_001798"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO575_001798"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6664790.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO575_001799"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO575_001799"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6664791.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO575_001800"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO575_001800"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO575_001801"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO575_001801"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6664792.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO575_001802"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO575_001802"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6664793.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO575_001803"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO575_001803"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6664794.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO575_001804"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO575_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6664795.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO575_001805"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO575_001805"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6664796.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDCDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO575_001806"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO575_001806"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6664797.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO575_001807"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO575_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6664798.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO575_001808"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO575_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6664799.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO575_001809"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO575_001809"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6664800.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO575_001810"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO575_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6664801.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO575_001811"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO575_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6664802.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO575_001812"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO575_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6664803.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO575_001813"
     CDS             complement(20900..21019)
                     /locus_tag="OO575_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR6664804.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO575_001814"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO575_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6664805.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO575_001815"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO575_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6664806.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25505)
                     /gene="hycC"
                     /locus_tag="OO575_001816"
     CDS             complement(23682..25505)
                     /gene="hycC"
                     /locus_tag="OO575_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6664807.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLF
                     KSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWV
                     IYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENA
                     TCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIA
                     CPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLR
                     KWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25542..26153)
                     /gene="hycB"
                     /locus_tag="OO575_001817"
     CDS             complement(25542..26153)
                     /gene="hycB"
                     /locus_tag="OO575_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6664808.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26278..26739)
                     /gene="hycA"
                     /locus_tag="OO575_001818"
     CDS             complement(26278..26739)
                     /gene="hycA"
                     /locus_tag="OO575_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6664809.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26951..27301
                     /gene="hypA"
                     /locus_tag="OO575_001819"
     CDS             26951..27301
                     /gene="hypA"
                     /locus_tag="OO575_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6664810.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27305..28177
                     /gene="hypB"
                     /locus_tag="OO575_001820"
     CDS             27305..28177
                     /gene="hypB"
                     /locus_tag="OO575_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6664811.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28168..28440
                     /gene="hypC"
                     /locus_tag="OO575_001821"
     CDS             28168..28440
                     /gene="hypC"
                     /locus_tag="OO575_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6664812.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28440..29561
                     /gene="hypD"
                     /locus_tag="OO575_001822"
     CDS             28440..29561
                     /gene="hypD"
                     /locus_tag="OO575_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6664813.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29558..30568
                     /gene="hypE"
                     /locus_tag="OO575_001823"
     CDS             29558..30568
                     /gene="hypE"
                     /locus_tag="OO575_001823"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6664814.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30642..32720
                     /gene="flhA"
                     /locus_tag="OO575_001824"
     CDS             30642..32720
                     /gene="flhA"
                     /locus_tag="OO575_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6664815.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32757..>33093)
                     /locus_tag="OO575_001825"
     CDS             complement(32757..>33093)
                     /locus_tag="OO575_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6664816.1"
                     /translation="SREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKRLDKCVF
                     GEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRPVPELPE
                     KYRPKKPRE"
BASE COUNT         7794 a         9229 c         8619 g         7451 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga ttgcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagccccag caggataatg
    24661 ccgatatttt ccagggtgtg gtaagccagc aggcgctgga tgttgtgttc catcaacgca
    24721 tacagaccac cgacgaacgc ggtgatcatg ccgagcacca gcagcgcgat cccccaccac
    24781 agcggtgcat taccgcccag cagtgacagg gttaacatgc ccagcaggcc aattttcatg
    24841 actaccgtag aaaacaacgc ggcagctggc gcagaggcgt tagcatgtgc ctgcggcacc
    24901 cagccgtgca gcggaataat cccagccagc aggccaaagc caatcacacc gagcagccag
    24961 atatcggaac cgagcggcag ctgttgcata cgcatatcca gcaggcgcag atccagcgtg
    25021 ccataacgct gccacagcag ccagcaagca atcaccagca gcagagtgcc aagacgcctc
    25081 agcgcaaacc acagtttgcc ctctttgctg ttgctggtga ggaacaccgc acacaaggcc
    25141 atgacttcgg ccattaccac gaacatgccg aggttgctgg cgatgacggc gcagacggcg
    25201 gcagccatca acatattgat ctgcaagccg ttgcacttca cctgcgcgtg gcgatgccag
    25261 tcaatgttat agaggctgac aaacagaccg caaaggccga gggtaatcag ccaaatcgcg
    25321 ttaagcgggg agatttgcac atcgtagctt accagcgaca gcgcaccgct cacgccaacc
    25381 gcgccagtca gtacagtgaa gcccgcggct gccgtataca gactaccaac cgcgccgcca
    25441 attccggcta tccagccact tagcgctttt tgaaaagaga aatgccagaa cagcggcggc
    25501 gacaaaccat gccacgccgc tattgatcag ggaaattgcg ctcatttagc ctctccactt
    25561 tgagcctgct gaaacaaggt gagatcgcca aagtccgtgt taaaggtcag ctcacgctta
    25621 cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt agtcgggcac
    25681 atccgcacac aggccggacc ttgttcatca aagctacaaa ggtcacattt gacggcgatc
    25741 gcgcgaatac ctggcaccca gtcaagcaat gtgctgacac gcgccggagc aggcggtgcc
    25801 ggtggcgctt tcggggtatt ggcgtttgcc ggaatatcca gcggacggct gccggaaaat
    25861 tcaattgcgc caaacgggca ggcgatgccg cacagtttgc agcttacgca cagactttca
    25921 ttcaactgca cggccccatc aacgcgggtg atggcgttaa ccgggcagac caccgcgcag
    25981 ggtgcatctt cacagtggtg acagagctgc ggcgcagatt ctttttcatt gagcatcact
    26041 ctcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca ggcggcctca
    26101 caggtgtggc agccgataca gagcgtggag tcggcaatta caaaacgatt caccaggcat
    26161 tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt ttcgacactc
    26221 atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag cctcagctca
    26281 tgctgccggg ctttgtccct ttaccagttg gcttaaattc accggcacat tgttttcaat
    26341 ggcggtgtat aagctgtcgt aaacgccagc gattttttcg agatagtgtt ccagaacctg
    26401 ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca gttgcgcctg
    26461 cgcaatccgc tgtttgtctt cgccatcttt tgtttcgacg taatactcga tggtgtggct
    26521 gttaaagcca tccgccaggg tgacgtaaat acgaaaatgt tcaaaaagga cgaaacagag
    26581 ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata acgtgatccc
    26641 ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt aggcgacgat gccgctgtgc
    26701 gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac tcgtttaaca
    26761 gagatgccag ctttaagcat tttttgtgcc aacttttaat ttattgttat taaagagatt
    26821 tttaagctaa agatgaattt cgtcgccgtg tcgacgtgtc atttcgacat catcgacatt
    26881 attcaccgca gggataatca acactggcgc aattattgct tgtagctggc aatagttaat
    26941 gggaggcgat atgcacgaaa taaccctctg ccaacgggca ctggaattga tcgaacagca
    27001 ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg gcgcattttc
    27061 ttgtgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg gcagcgtggc
    27121 ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa tgctggtgtg aaacatgcca
    27181 acagtatgtg acgctactga cccagcgcgt ccgccgctgt ccacagtgtc atggtgacat
    27241 gctacagatt gtggcagacg acggtttaca gattcggcgg atagaaatag accaggagtg
    27301 agcgatgtgt acaacatgcg gttgcggtga aggcaacctg tatatcgagg gtgatgaaca
    27361 taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc cgcagatgaa
    27421 aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag gcgacctgca
    27481 ttacggtcat ggcgaagcgg gcactcacac accgggcatg agccagcgtc ggatgctgga
    27541 agtcgaaatt gacgtgctgg acaaaaacaa ccgtctggct gaacgcaacc gcgcgcgctt
    27601 tgctgcccgc aagcaactgg tgctcaacct ggtttccagc cctggttccg gtaaaaccac
    27661 cctgctgacg gaaaccttaa tgcgcctgaa agacagcgtt ccgtgcgcgg ttattgaagg
    27721 cgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggtacac cagcgattca
    27781 ggtgaacacc ggtaaaggct gccatcttga cgcacagatg attgccgacg ccgcaccgcg
    27841 tctgccactg gacgataacg gtattctgtt tatcgaaaac gttggcaacc tcgtatgccc
    27901 ggccagcttc gatctcggtg aaaaacacaa agtagcggtg ctttccgtta ccgaaggtga
    27961 agacaaaccg ctgaaatatc cgcatatgtt tgccgccgcc tcgctgatgc tgctcaacaa
    28021 agttgacctg ttgccgtatc tcaactttga cgttgagaag tgcatcgcct gcgcccgcga
    28081 agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggcgaag ggatggacca
    28141 gtggctgaac tggctggaga cacagcgatg tgcataggcg ttcccggcca gatccgcacc
    28201 attgacggca accaggcgaa agtcgacgtc tgcggcattc agcgcgatgt cgatttaacg
    28261 ttagtcggca gctgcgatga aaacggtcag ccgcgcgtgg gccagtgggt actggttcac
    28321 gttggctttg ccatgagcgt aattaatgaa gccgaagcac gcgacactct cgacgcctta
    28381 caaaacatgt ttgacgttga gccggatgtc ggcgcgctgt tgtatggcga ggaaaaataa
    28441 tgcgttttgt tgatgaatat cgcgcgccgg aacaggtgat gcagttaatt gagcatctgc
    28501 gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt atggaagtgt
    28561 gtggcggtca tacccacgcc atttttaaat tcggcctcga ccagttactg cctgaaaacg
    28621 ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact gccgatgggt agaatcgaca
    28681 cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc gacgccatgc
    28741 gcgtaccagg gaaacaggga tcgctgttgc aggcaaaagc acgcggtgcc gatgtgcgca
    28801 tcgtttactc gccgatggat gcgttgaaac tggcgcagga gaatccaacc cgcaaagtgg
    28861 tgttcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact ctgcaacagg
    28921 cgaaagcgcg tgatgtgcag aatttttact tcttctgcca gcacattacg cttatcccga
    28981 cgctgcgcag tttgctggaa cagccggata acggtatcga cgcgttcctc gcgccgggcc
    29041 acgttagtat ggttatcggc actgatgcct ataattttat cgccagcgat tttcatcgtc
    29101 cgctggtggt cgctggtttc gaaccccttg atctactgca aggcgtggtc atgctggtgg
    29161 agcagaaaat agcggcccac agcaaggtag agaatcagta tcgtcgggtg gtaccggatg
    29221 ccggtaacct actggcgcaa caggcgattg ccgatgtgtt ctgtgtcaac ggcgacagcg
    29281 aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg gattatcaac
    29341 gattcgatgc cgaagcacat ttccgcccgg caccgcagca ggtctgcgat gacccgcgcg
    29401 cgcgttgtgg tgaggtatta acgggcaaat gtaagccgca tcaatgcccg ctgtttggta
    29461 acacctgtaa tcctcaaacc gcatttggtg cactgatggt ctcctccgaa ggagcgtgcg
    29521 ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc aactcgccca
    29581 cggcagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg aagcctttgc
    29641 caacccgtgg ctggcagagc aggaagatca ggcacgtctt gatctggcgc agctggtagc
    29701 ggaaggcgac cgtctggcgt tctccaccga cagttacgtc attgacccgc tgttctttcc
    29761 tggcggtaat atcggcaagc tggcgatttg cggcaccgcg aatgacgttg cgggcagtgg
    29821 cgctattccg cgctatctct cctgtggctt tatcctcgaa gaaggattgc cgatggagac
    29881 actgaaagcc gtagtgacca gcatggcaga aaccgcccgc acggcaggca ttgccatcgt
    29941 tactggcgat actaaagtgg tgcagcgcgg cgcggcagat aaactgttta tcaacaccgc
    30001 tggcatgggc gcaattccgg cgaatattca ctggggcgca cagacgctaa ccgcaggcga
    30061 tgtattgctg gttagcggta cactcggcga ccacggggcg actatcctta acctgcgtga
    30121 gcagctgggg ctggatggcg aactggtcag cgactgcgcg gtgctgacgc cgcttattca
    30181 gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg gtggtgtaaa
    30241 cgcggtggtt catgagttcg cggcagcctg cggttgtggt attgaaattt ctgaatcagc
    30301 gctgccggtt aaacctgccg tgcgcggcgt ttgcgaattg ctgggactgg acgccctgaa
    30361 ctttgccaac gaaggcaaac tggtgatcgc cgttgaacgc aacgcggcag agcaagtgct
    30421 ggcagcgtta cattcccatc cactggggaa agacgcggcg ctgattggtg aagtggtgga
    30481 acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa cgaaccctcg atttaccaca
    30541 cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag ttagtcaatg
    30601 accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat acaccgatga
    30661 gtgatctcgg acagcaaggg ttgttcgaca tcactcggac actattgcag cagcccgatc
    30721 tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc gccgacaacg
    30781 cggctattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgcg tcgcgtgaaa
    30841 aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg gtacgcagca
    30901 ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga attttgtgaa acctggccgc
    30961 agctggccgc aggtgggcta taccccaaat ttggtcacta ttgcctgatg ccactggcgg
    31021 cgggagggca tatttttggt ggctgtgaat ttattcgtta tgacgagcgt ccctggagcg
    31081 aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc accgaacaaa
    31141 tccagagccg cgtcgtcaac aatgtcgact atgagttgtt atgccgggag cgcgataact
    31201 tccgcatcct ggttgccatt accaacgcgg tgctttcccg cctggatatg gacgaactgg
    31261 tcagcgaagt cgccaaagaa atccattact atttcgacat tgacgatatc agcattgtct
    31321 tacgcagcca ccgtaaaaac aaactcaaca tctactccac tcactatctt gataaacaac
    31381 atcccgccca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa cgcgtgttca
    31441 aaagtaaaga gatgctgctg atcaatctct acgagcggga cgatttagcc ccctatgaac
    31501 gcatgttgtt cgacacctgg ggcaaccaga ttcaaacctt gtgcctgtta ccgctgatgt
    31561 ctggcgacac catgctgggc gtgctgaaac tggcgcaatg cgaagagaaa gtgtttacca
    31621 ctaccaatct gaatttgctg cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg
    31681 ccctcgccta tcaggaaatc catcgtctga aagaacggct ggttgatgaa aacctcgccc
    31741 tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc cgcagcgaag
    31801 ccatgtacag cgtgcttaaa caagttgaaa tggtggcgca aagtgacagt accgtgctga
    31861 tcctcggtga aactggctcg ggtaaagagc tgattgcccg tgcgatccat aatctcagtg
    31921 ggcgtaataa tcgccgcatg gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg
    31981 aaagcgatct gtttggtcat gagcgtgggg cttttaccgg tgccaacgcc cagcgcattg
    32041 gtcgttttga actggcggat aaaagctctc tgttcctcga cgaagtgggc gatatgccac
    32101 tggagttaca gccgaagttg ctgcgtgtat tgcaggaaca ggagtttgaa cgtctcggca
    32161 gcaacaaaat cattcagacg gacgtacgtt taattgccgc gactaaccgc gatctgaaaa
    32221 aaatggtcgc cgaccgagag ttccgtagcg atctctatta ccgcctgaac gtattcccga
    32281 ttcacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg aaagccttta
    32341 ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattcctgcc gagacgctgc
    32401 gcaccttgag caacatggag tggccgggta acgtacgcga actggaaaac gtcattgagc
    32461 gcgcagtatt gctaacacgc ggcaacgtgc tgcagctgtc attgccagat attgctttac
    32521 cggagcctga aacgccgcct gccgcaacgg ttgtcgccca ggagggcgaa gatgaatatc
    32581 agttgattgt gcgcgtgctg aaagaaacca acagcgtggt tgccgggcct aaaggcgctg
    32641 cgcaacgtct ggggctgaaa cgcacgaccc tgctgtcacg gatgaagcgg ctgggaattg
    32701 ataaatcggc attgatttaa ctgcaaattg ccggacagat ctgcctgtcc ggcatactat
    32761 tcacgaggtt ttttcggacg atatttttcc ggcagttctg gcaccggacg cttgtcatcg
    32821 atgagatgac gcacggttaa gatcggatga cgccacagca ttctcggccc ggcccaacgc
    32881 ataatctgtt tcatctcttc acgctttgca ggctggtaac agtgcaccgg acactgctta
    32941 caggctggtt tctcttcgcc gaacacacat ttatccagcc gcttttgcgc gtaaacaaac
    33001 aacgcctcgt aatgctccgg ctccgctgac gcctgcgggc atttcgcttg ataaagatcg
    33061 atcatttttt taatcgtcag tttttcacga gag
//
LOCUS       DAJTYM010000038        33060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTYM010000038 DAJTYM010000000
VERSION     DAJTYM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..267
                     /locus_tag="OO575_001826"
     CDS             <1..267
                     /locus_tag="OO575_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="responsible for the influx of magnesium ions;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR6664817.1"
                     /translation="HNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLV
                     ASSYGMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(308..688)
                     /locus_tag="OO575_001827"
     CDS             complement(308..688)
                     /locus_tag="OO575_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6664818.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(702..1118)
                     /locus_tag="OO575_001828"
     CDS             complement(702..1118)
                     /locus_tag="OO575_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664819.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(1177..2067)
                     /gene="rarD"
                     /locus_tag="OO575_001829"
     CDS             complement(1177..2067)
                     /gene="rarD"
                     /locus_tag="OO575_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6664820.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(2119..2586)
                     /locus_tag="OO575_001830"
     CDS             complement(2119..2586)
                     /locus_tag="OO575_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6664821.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2751..3620
                     /gene="pldA"
                     /locus_tag="OO575_001831"
     CDS             2751..3620
                     /gene="pldA"
                     /locus_tag="OO575_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6664822.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3753..5582
                     /gene="recQ"
                     /locus_tag="OO575_001832"
     CDS             3753..5582
                     /gene="recQ"
                     /locus_tag="OO575_001832"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6664823.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5646..6266
                     /gene="rhtC"
                     /locus_tag="OO575_001833"
     CDS             5646..6266
                     /gene="rhtC"
                     /locus_tag="OO575_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6664824.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6328..6948)
                     /gene="rhtB"
                     /locus_tag="OO575_001834"
     CDS             complement(6328..6948)
                     /gene="rhtB"
                     /locus_tag="OO575_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6664825.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7059..8081
                     /gene="pldB"
                     /locus_tag="OO575_001835"
     CDS             7059..8081
                     /gene="pldB"
                     /locus_tag="OO575_001835"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6664826.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8089..8889
                     /gene="yigL"
                     /locus_tag="OO575_001836"
     CDS             8089..8889
                     /gene="yigL"
                     /locus_tag="OO575_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6664827.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8965..9864
                     /gene="bioP"
                     /locus_tag="OO575_001837"
     CDS             8965..9864
                     /gene="bioP"
                     /locus_tag="OO575_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6664828.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9752..10705)
                     /gene="metR"
                     /locus_tag="OO575_001838"
     CDS             complement(9752..10705)
                     /gene="metR"
                     /locus_tag="OO575_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6664829.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10941..13202
                     /gene="metE"
                     /locus_tag="OO575_001839"
     CDS             10941..13202
                     /gene="metE"
                     /locus_tag="OO575_001839"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6664830.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13241..14056)
                     /locus_tag="OO575_001840"
     CDS             complement(13241..14056)
                     /locus_tag="OO575_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6664831.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            14318..15079
                     /gene="udp"
                     /locus_tag="OO575_001841"
     CDS             14318..15079
                     /gene="udp"
                     /locus_tag="OO575_001841"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6664832.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            15220..16647
                     /gene="rmuC"
                     /locus_tag="OO575_001842"
     CDS             15220..16647
                     /gene="rmuC"
                     /locus_tag="OO575_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6664833.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            16742..17497
                     /gene="ubiE"
                     /locus_tag="OO575_001843"
     CDS             16742..17497
                     /gene="ubiE"
                     /locus_tag="OO575_001843"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6664834.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            17511..18116
                     /gene="ubiJ"
                     /locus_tag="OO575_001844"
     CDS             17511..18116
                     /gene="ubiJ"
                     /locus_tag="OO575_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6664835.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18113..19753
                     /gene="ubiB"
                     /locus_tag="OO575_001845"
     CDS             18113..19753
                     /gene="ubiB"
                     /locus_tag="OO575_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6664836.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19832..20101
                     /gene="tatA"
                     /locus_tag="OO575_001846"
     CDS             19832..20101
                     /gene="tatA"
                     /locus_tag="OO575_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6664837.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20105..20620
                     /gene="tatB"
                     /locus_tag="OO575_001847"
     CDS             20105..20620
                     /gene="tatB"
                     /locus_tag="OO575_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6664838.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            20623..21399
                     /gene="tatC"
                     /locus_tag="OO575_001848"
     CDS             20623..21399
                     /gene="tatC"
                     /locus_tag="OO575_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6664839.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            21441..22223
                     /gene="tatD"
                     /locus_tag="OO575_001849"
     CDS             21441..22223
                     /gene="tatD"
                     /locus_tag="OO575_001849"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6664840.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22220..22708)
                     /gene="rfaH"
                     /locus_tag="OO575_001850"
     CDS             complement(22220..22708)
                     /gene="rfaH"
                     /locus_tag="OO575_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6664841.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22875..24368
                     /gene="ubiD"
                     /locus_tag="OO575_001851"
     CDS             22875..24368
                     /gene="ubiD"
                     /locus_tag="OO575_001851"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6664842.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            24414..25115
                     /gene="fre"
                     /locus_tag="OO575_001852"
     CDS             24414..25115
                     /gene="fre"
                     /locus_tag="OO575_001852"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6664843.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(25307..26470)
                     /gene="fadA"
                     /locus_tag="OO575_001853"
     CDS             complement(25307..26470)
                     /gene="fadA"
                     /locus_tag="OO575_001853"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6664844.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(26480..28669)
                     /gene="fadB"
                     /locus_tag="OO575_001854"
     CDS             complement(26480..28669)
                     /gene="fadB"
                     /locus_tag="OO575_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6664845.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28859..30190
                     /gene="pepQ"
                     /locus_tag="OO575_001855"
     CDS             28859..30190
                     /gene="pepQ"
                     /locus_tag="OO575_001855"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6664846.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            30190..30804
                     /locus_tag="OO575_001856"
     CDS             30190..30804
                     /locus_tag="OO575_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6664847.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30843..32294
                     /gene="trkH"
                     /locus_tag="OO575_001857"
     CDS             30843..32294
                     /gene="trkH"
                     /locus_tag="OO575_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6664848.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            32306..32851
                     /gene="hemG"
                     /locus_tag="OO575_001858"
     CDS             32306..32851
                     /gene="hemG"
                     /locus_tag="OO575_001858"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6664849.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7788 a         8347 c         8943 g         7982 t
ORIGIN      
        1 cataacgaat ccctgttcca gaaggtgaac ttcctgatgc aggcggcaat gggttttatc
       61 aacatcgagc agaaccgcat catcaaaatt ttctcggtgg tatccgtcgt gttcctgccg
      121 ccaacgctgg ttgcttccag ctacgggatg aactttgagt ttatgccaga actgaagtgg
      181 agtttcggct accctggcgc gattatcttt atgatcctcg cgggcctggc accgtatctg
      241 tactttaagc ggaagaactg gttgtaaaaa gaaggcggtg gcttagcctg gctaagccac
      301 ctgttattca aaggctccag gtatttaacc cttttacctc tttctcataa aaccatttgt
      361 tcgtgttaac agcaacatag gctgctacgg caattcccag aatgttaacg ccaattaatg
      421 caccaacaaa tagagataga atgccaagta atagaactat aattgctttt ttccataagc
      481 ccagaacaaa taaatagatc caggaacaga agaaagcaat gaaattcatt tgaatagtta
      541 atcgttgtct tacttttaat gctttaaatg ctgctttata ttctggtgtt gctccccaga
      601 atccaggaaa gccatgttga tcataaaaat taaatcggta tttccatttt tcacttaatg
      661 aatcatcgtt catatattcc ttactcataa atactccata actattgttt tgatgaatca
      721 gtaggtgcaa gcattagcat actgaaagtg gaaaaataac aaatcaaaaa aatcatcgaa
      781 ccattgcctg aacaggcaaa atcttcggct atcattgtga tgatagagat gatatatact
      841 gctaatgtac caaaaacata aatttttata tatatgaaac cactatcacg gagtcgttgg
      901 caattcatgt tgatgacgag ataatggagt gcgatggtag agactataac aagaaaacct
      961 gcttccccat cgttaaaaaa gataataaga aaggcaaaaa tgaaatttat taaaataaat
     1021 aaaaatatat atcggtgtct ggaaatctta ccgttagatg ttgggataaa catacgtaac
     1081 ataaatttta catccttgtg tgagtctcct gtcagcatgg caatatgccc actctcatac
     1141 aagagtcggc atattttttc agaatatatt tattttttat ttggacgttc tacgctgcgt
     1201 ataaatcgcg tccatcacaa aaattgccag cgccacccaa ataaaggtga aagtcaccat
     1261 cttatcggca cccggttttt caccataaaa cgtcacagcc agcaggaaca tcagcgtcgg
     1321 gccaatgtac tgaaaaaagc ctaacgttga gagacgcagg cgcgtggcag cggcagtaaa
     1381 acacaacagc ggtacggtag tgacaatacc ggcggcgatc agcagtaaat tcagcgacat
     1441 cgggttttgc cccatatggc tggttgaact gtcggcaata gcaaacaggt aaatcgccgc
     1501 cacgggcagc agccacatgg tttcaattaa catgccggtt tgtgcttcta ccgcaatctt
     1561 cttgcgcacc aggccgtaga aggcaaaact aaatgccagc cccagcgcga taataggtag
     1621 cgaaccaaaa gtccacagct ggaccaacac gccacatatc gccagaatca ccgccagcca
     1681 ttgcatccgg cggaatcgct cgccgaggaa aatcatcccc agcacaatgt tcaccagcgg
     1741 gttaataaag taaccaaggc tcgcttccag catatggtga ttgttcaccg cccagataaa
     1801 cagtagccag ttgccaccaa tcagtacggc agagactgcc agcataaaaa ttttctgtgg
     1861 cgtctgaatc agcgttttta aataggacca ctggcggcaa atgctcatca gcaccaccat
     1921 aaagaaaaac gaccagatca cgcgatgcgt caggatctca tcggcgggca cgtagtaaat
     1981 cagcttgaag tatgctggcg caatacccca aataaaataa gcggcaagag cgagtaatac
     2041 gccctgccgc gtttgttttg catccatcgg gaatactcat ttttaattgg taacagcagt
     2101 ttacctgctt ttatgtcttc aacctaccat ataggtggcg gtggcactgg caatataaag
     2161 ctgttcttca ttgtgtaatt caacgcgggc gacggcgact ttattgcctg cacgcaacag
     2221 gctactggta gcagtaaaac gctcgcccct gcctgggcgc agataatcaa cgcgaagatc
     2281 aatggtcccc atccgcgata gccgctggcg tagttcatct tcactgatgg tttcgtggcg
     2341 ggttaaggta cttcccacgc acaccagacc ggcggcgaca tccagcgccg acgcaatgac
     2401 tccgccgtgc aaaatgcttt gcgcccagtt acccaccatc atcggctgat ttttaaaggc
     2461 cagctgtgcg aactcttttt cgtaacgctc cagttccatc cccaatgcgc ggttaaatgg
     2521 catgtgataa acaaacatct cacccactaa tttcagggct tgttcagcgg tcagtacggc
     2581 agacatatca tccttacact tcattggtta atgaaatgtt gattttatgc ttctttgttg
     2641 ttggtttcta ctttaggaag ggataactaa cggcaatgga gttaagtatg taaaatagcc
     2701 cgcagaaaaa cattcacctt atcaataatt cgttacggag aacacgaccg atgcggactc
     2761 tgcagggctg gttgttgccg gtgtttatgt tgcctatggc agtatatgca caagaggcaa
     2821 cggtgaaaga ggtgcatgac gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc
     2881 aggagcatga caatccgttc acgctctatc cttatgacac caactacctc atctacaccc
     2941 aaaccagcga tctgaataaa gaagcgattg ccagttacga ctgggcggaa aatgcgcgta
     3001 aggatgaagt aaagtttcag ttgagcctgg catttccgct gtggcgtggg attttaggcc
     3061 caaactcggt gttgggtgcg tcttatacgc aaaaatcctg gtggcaactg tccaatagcg
     3121 aagagtcttc accgtttcgt gaaaccaact acgaaccgca attgttcctc ggttttgcca
     3181 ccgattaccg ttttgcaggt tggacgctgc gcgatgtgga gatggggtat aaccacgact
     3241 ctaacgggcg ttcagacccg acctcccgca gctggaaccg cctttatact cgcctgatgg
     3301 cagaaaacgg taactggctg gtagaagtga agccgtggta tgtggtgggt aatactgacg
     3361 ataacccgga tatcaccaaa tatatgggtt actaccagct taaaatcggc tatcacctcg
     3421 gtgatgcggt gctcagtgcg aaaggacagt acaactggaa caccggctac ggcggcgcgg
     3481 agttaggctt aagttacccg atcaccaaac atgtgcgcct ttatactcag gtttacagcg
     3541 gctatggcga atcgctcatc gactataact tcaaccagac ccgtgtcggt gtaggggtta
     3601 tgctaaacga cttgttttga tgaacggttg agtggttggc aaatctggaa tccagcatcc
     3661 aggattaccc tctcagagac taaaagcatt gcagtttctc gcgcaggcgc tgaaaatagc
     3721 gcctgttttt atttcaggca atcggggtga atgtggcgca ggcggaagtg ttgaatctgg
     3781 agtccggagc taaacaggtt ttacaagaaa cctttggcta ccaacagttt cgccccggcc
     3841 aggaagaaat tatcgacact gtgctttccg gtcgcgattg cctggttgtt atgcccactg
     3901 gtggcggaaa atccctttgc tatcaaattc ctgccttatt gctaaacggc cttaccgtgg
     3961 ttgtttcacc gctgatttcg ttgatgaaag atcaggtgga tcaactacag gccaacggcg
     4021 tggcggcggc gtgccttaac tcgacgcaaa cccgcgaaca gcaacttgaa gtgatgacag
     4081 gctgccgcac cgggcaaatt cgcttactgt atatcgcccc ggaacgcctg atgctggata
     4141 actttcttga gcatctggcg cactggaatc cggtgttatt agccgtcgat gaagcgcact
     4201 gtatctccca atggggtcac gatttccgcc cggaatatgc cgcgctcggt cagttgcgcc
     4261 agcggttccc gacgctgccg tttatggcgc tgaccgccac agccgacgac accacgcgcc
     4321 aggatatcgt gcgcctgctg gggctgaacg atccgctgat tcaaatcagc agttttgacc
     4381 gtccgaatat tcgctacatg ctgatggaga aattcaaacc gctcgatcag ttgatgcgct
     4441 acgtgcagga acagcgcggt aagtctggca ttatctactg caacagccgg gcgaaagtag
     4501 aagacaccgc tgcgcgcctg caaagcaggg gtattagcgc ggcggcctat catgccgggc
     4561 tggaaaataa tgtccgcgcc gacgtgcagg agaaattcca gcgcgatgat ctgcaaattg
     4621 tggtggcgac ggtggcgttc ggcatgggca tcaataaacc aaacgttcgc ttcgtggtcc
     4681 actttgatat tccgcgcaat atcgaatcct attatcagga aaccggtcgc gccgggcgtg
     4741 atggcctgcc tgcggaagcg atgctgtttt acgatccggc tgatatggcg tggctgcgcc
     4801 gttgtctgga agagaagccg caggggcagt tgcaggatat cgagcgccac aaactcaatg
     4861 cgatgggcgc gtttgccgaa gcgcaaactt gccgtcgtct ggtattgctg aactattttg
     4921 gcgaagggcg tcaggagccg tgcgggaact gcgatatctg cctcgatccg ccgaaacagt
     4981 acgacggttc aaccgatgct cagattgccc tttccaccat tggtcgtgtg aatcagcggt
     5041 ttgggatggg ttatgtggtg gaagtgattc gtggtgctaa taaccagcgt atccgcgact
     5101 atggtcatga caaactgaaa gtctatggta tgggccgtga taaaagccat gaacattggg
     5161 tgagcgtgat ccgccagctg attcatctcg gcctggtgac gcaaaatatt gcccagcatt
     5221 ctgccctaca actgacagag gccgcgcgcc cggtgctgcg cggcgaatcc tctttgcaac
     5281 ttgccgtgcc gcgtatcgtg gcgctcaaac cgaaagcgat gcagaaatcg ttcggcggca
     5341 actatgatcg caaactgttc gccaaattac gcaaactgcg taaatcgatt gccgatgaaa
     5401 gtaatgtccc gccgtacgtg gtgtttaacg acgcaacctt gattgagatg gctgaacaga
     5461 tgccgatcac cgccagcgaa atgctcagcg ttaacggcgt tgggatgcgc aagctggaac
     5521 gctttggtaa accgtttatg gcgctgattc gtgcgcatgt cgatggcgac gacgaagagt
     5581 agtcagcagc ataaaaaagt gccagtatga agactccgta aacgtttccc ccgcgagtca
     5641 aatgtatgtt gatgttattt ctcaccgtcg ccatggtgca cattgtggcg cttatgagcc
     5701 ccggtcccga tttctttttt gtctctcaga ccgctgtcag tcgttcccgt aaagaagcga
     5761 tgatgggcgt gctgggcatt acctgcggcg taatggtttg ggctgggatt gcgttgcttg
     5821 gcctgcattt gattatcgaa aaaatggcct ggctgcatac gctgattatg gtaggcggtg
     5881 gcctgtatct ctgctggatg ggttaccaga tgctacgcgg tgcactgaaa aaagaggctg
     5941 tatctgcgcc tgcaccacag gttgagctgg cgaaaagcgg gcgcagtttc ctgaaaggct
     6001 tactgaccaa tctcgccaat ccgaaagcga ttatctattt tggttcggta ttctcattgt
     6061 ttgtcggcga taacgttggc actaccgagc gctggggcat tttcgcgttg attattgtcg
     6121 aaacactggc gtggtttacc gtcgttgcca gtctgtttgc cctgccgcaa atgcgccgtg
     6181 gttatcaacg tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg
     6241 gcattcattt gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg
     6301 cgtttacgcc gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc
     6361 caccagcata aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt
     6421 aatccataga gcaatccttt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac
     6481 cacaatagtg gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat
     6541 gaattgcgga aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa
     6601 aactgcgcgc tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taagatcaat
     6661 tgcgccagcg gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca
     6721 ctttaacact tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac
     6781 cagcacaata tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg
     6841 ataaccgtgg ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct
     6901 taaaatgatc gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg
     6961 gtgtgtctat ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgcttaa
     7021 aaaaacgatt ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact
     7081 gggaaacaag agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc
     7141 gtcagcgtga tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt
     7201 ttcgcgcaca gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg
     7261 taaaatatgc ggaactggcc tacgacctgt tccatttggg gtttgatgtc ttaatcatcg
     7321 accatcgcgg gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta
     7381 atcgctttaa tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg
     7441 gtccgtggcg taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat
     7501 ttctgcaacg ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga
     7561 tcgtgattcg tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc
     7621 cacgtttccg tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta
     7681 tcaacgtact gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg
     7741 acccaacgat tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg
     7801 gcgaacaggt gttagccggt gcgggtgatg acgccacgca aacgcttctc ttacaagctg
     7861 aagaggaacg cgtggtggat aaccgcatgc acgaccgttt ttgtgaactc cgcaccgccg
     7921 cgggccatcc tgtcgaagga ggacggccgt tggtaataaa aggtgcttac catgagatcc
     7981 tttttgaaaa ggacgcaatg cgctcagtcg cgctccacgc catcgttgat tttttcaaca
     8041 ggcataactc acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt
     8101 gttgcgtctg atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc
     8161 aaagaaactc tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt
     8221 caccacgttg atgtggggca aattcgcgat aatctggaga ttaagtctta catgattacc
     8281 tccaatggtg cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat
     8341 cgcgacattg ccagcgatct gtttggcgta gtcaacgaca atccggacat cattactaac
     8401 gtttatcgcg acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt
     8461 aaagaagcgg tgttcaaata tgcgctgtat gagccgggat tactggagcc ggaaggcatc
     8521 agcaaagtgt ttttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt
     8581 aacgctcgct ggggcgatcg cgtcaacgtc agtttctcta ccttaacctg tctggaagtg
     8641 atggcgggcg gcgtgtcgaa aggccatgcg ctggaagcag tggcgaagaa actgggctac
     8701 agcctgaagg attgtattgc gtttggtgac gggatgaacg acgccgaaat gctgtcgatg
     8761 gcggggaaag gctgcattat gggcagtgcg caccagcgtc tgaaagatct gcatccggag
     8821 ctggaagtta ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat
     8881 ttatcgtaat cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac
     8941 tcgttaatca aagagttttc cattgtggcg ttacttatca tcaccacgat tctgtgggcc
     9001 ttctccttta gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg
     9061 ctggtgcgcg ttggcttggc ggcactcgtt tttctgccgt ttctgcgtac ccgtagcaat
     9121 agcctgaaaa cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat
     9181 atgctgagtt tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta
     9241 ctgacgccgc tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg
     9301 ggctatgctt ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag
     9361 gtcaccgacc atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc
     9421 atcggcatgg tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gtataacgcc
     9481 tttgcgtggt tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga
     9541 aatgcgcaga aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg
     9601 gtggcttccg ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga
     9661 acgctgggca ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc
     9721 tggcaccaac agccgcactg gccaacgttt attacaggcg cgctggtgat cctggcctca
     9781 ctgtgggtgc atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg
     9841 cgtgattgcg cgctgagcga ataaacgctt ccgtaactgg ctgacgctgc tcgccatcgc
     9901 gcacggcggc gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac
     9961 cctggcgctc aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg
    10021 caaccatttg aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg
    10081 ccggctgaag aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaattaata
    10141 gcgtctcgct ggcgagatct tccggtgtaa tacgcgtttt tgccgccagt ggatggtcag
    10201 gtgctaacac caggcgtact tcataatcga acatcggcga ataatgcagg ccactgcgcg
    10261 gcaggatatc ggaggtcatc accagatcca gctcgccctg ttgcaacgcg ggctgcgggt
    10321 caaacgtcac gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttc
    10381 ctaaagcggg tgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg
    10441 tctgctgcgg ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg
    10501 ccagttgcaa caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa
    10561 atagccggaa gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg
    10621 tctgatgcaa cgttgccgca gcggctgcga gcgagccgca gttccgcaac gcttgtagcg
    10681 ttttcaggtg ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct
    10741 tgaggaatat acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcat
    10801 tcgtctttta aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt
    10861 aagctcccgg cgatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac
    10921 atataattag aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg
    10981 gcctgcgtcg cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg
    11041 aagaactgct gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag
    11101 cgggtatcga cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca
    11161 gtttgctgct gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg
    11221 acaccctgtt ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg
    11281 aaatgaccaa atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc
    11341 aacagttcaa actgacttgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctga
    11401 gccacaacgt taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga
    11461 aaggtgaaca gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag
    11521 tgctggcaga actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg
    11581 tactggaact accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg
    11641 gacaggtgaa actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga
    11701 ttactgcgct gcctgttcag gggctgcatg tggaccttgt acatggtaaa gataacgttg
    11761 ttgaactgca caagcgcctg ccttctgact ggctgctgtc tgcgggtctg atcaatggtc
    11821 gtaacgtctg gcgcgccgat cttactgaga aatatgcgca aattaaggac attgtcggca
    11881 aacgtgattt gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg
    11941 tggaaacgcg tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc
    12001 atgaactggc actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt
    12061 ggagcgcccc aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa
    12121 agcgtctggc ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg
    12181 ctgaagccca gcgtgcgcgt tttaaactgc cagcgtggcc gaccaccacg attggttcct
    12241 tcccgcaaac cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg
    12301 ccaacaacta ccgcacgggc attgcggaac atatcaagca ggccattgtt gagcaggaac
    12361 gtttgggact ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact
    12421 ttggcgagca cctcgacgga tttgtcttta cgcaaaacgg ttgggtacag agctacggtt
    12481 cccgctgcgt gaagccaccg attgtcattg gtgacattag ccgcccggca ccgattaccg
    12541 tggagtgggc gaagtatgcg caatcgctga ccgacaaacc ggtgaaaggg atgctgacgg
    12601 ggccggtgac catactctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg
    12661 ccaaacagat tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg
    12721 gcatcatcca gattgacgaa ccggcgctgc gcgaaggttt accgctgcgt cgtagcgact
    12781 gggatgcgta tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag
    12841 atgacacaca aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga
    12901 ttgcggcgct ggacgcagac gtcatcacca tcgaaacctc gcgttccgac atggagttgc
    12961 tggagtcgtt tgaagagttt gattatccaa atgaaatcgg tcctggcgtc tatgacattc
    13021 actcgccaaa cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac
    13081 gcattccggc agagcgcctg tgggtcaacc cggactgtgg cctgaaaacg cgcggctggc
    13141 cagaaacccg cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcgggggt
    13201 aaatccaaac cgggtggtaa taccacccgg tcttttctca ttacagcgac ttcttcccac
    13261 catactgctt aaaccattcc agcatacgct gccagccatc tttcgcagat tcggcatgat
    13321 agctcgggcg ataatcagcg ttgaatgcat gcccggcatc cgggtacacg ataatctctg
    13381 ctttcgcatt agcagcccgc agcgcctggc gcatcgtttc aacgctctcc tgcggaatgc
    13441 tgttatcctg accaccatat aagccgagaa ccggcgcgtt aagatcggtt gcgatatcaa
    13501 caggttgttt cggtgaattc agcgacttgt cgcccgtcag tttgccgtac cacgccactg
    13561 cggcttttag ctgtggatta tgcgcggcat acagccaggt gatacgtcca ccccagcaga
    13621 atccggtgat cattaaacga tgaacatcgc cgccgttgcg ggaagcccaa ctggcgacat
    13681 gatcgagatc ggccagcacc tgcgagtcag gcacttttgc taccagaccg ctaagcaacg
    13741 tggggatatc ggcaaaatca ttcggatcgc cttcgcggaa gtaaagttca ggtgcgatag
    13801 ccagataccc ctccagcgcc agacggcgac aaatgtcgcg gatatgttca tgcacgccaa
    13861 aaatttcctg cactacaatg accactggca gtgggccatc gctttgcttt ggtctggcat
    13921 ggtaagcagg catgttatcc ccttgtgaag ggatagaggt gaagcccgcc acaatcgcgt
    13981 cgtccggggt ctgaacgatg gtcgaagcga gaggcgatgc agcaggtgca aatccagatt
    14041 gttgtgttgt tgccatggta ttctccgtac ccttataaaa atgttgcgca atgttaacta
    14101 tagtcagcat gcaacaaatc acattgcctg aatcggctca tcttttatgc agtcctgcag
    14161 aatgaagagt gatttatgtg atatgaatca cttttggtgg gtaaatttat gcaacgcatt
    14221 tgcgtcatgg tgatgagtat cacgaaaaaa tgttaaaccc ttcggtaaag tgtctttttg
    14281 cttcttctga ctaaaccgat tcacagagga gttgtatatg tccaagtctg atgtttttca
    14341 tctcggcctc actaaaaacg atttacaagg ggctacgctt gccatcgtcc ctggcgaccc
    14401 ggatcgtgtg gaaaagatcg ccgcgctgat ggataagccg gttaagctgg catctcaccg
    14461 cgaattcact acctggcgtg cagagctgga tggtaaacct gttatcgtct gctctaccgg
    14521 tatcggcggc ccgtctacct ctattgctgt tgaagagctg gcacagctgg gcattcgcac
    14581 cttcctgcgt atcggtacaa cgggcgctat tcagccgcat attaatgtgg gtgatgtcct
    14641 ggttaccacg gcgtctgtcc gtctggatgg cgcgagcctg cacttcgcac cgctggaatt
    14701 cccggctgtc gctgatttcg aatgtacgac tgcgctggtt gaagctgcga aatctattgg
    14761 cgcgacaact cacgttggcg tgacagtttc ttctgatacc ttctacccag gtcaggaacg
    14821 ttacgatact tactctggtc gcgtagttcg tcactttaaa ggttctatgg aagagtggca
    14881 ggcgatgggc gtaatgaact atgaaatgga atctgcaact ctgctgacca tgtgtgcaag
    14941 tcagggcctg cgtgccggta tggtagcggg tgttatcgtt aaccgcaccc agcaagagat
    15001 cccgaatgct gagacgatga aacaaaccga aagccatgcg gtgaaaatcg tggtggaagc
    15061 ggcgcgtcgt ctgctgtaat tctcttctcc tgtctgaagg ccgacgcgtt cggccttttg
    15121 tatttttgcg tagcgcctcg caggaaatgc ctttccaact ggacgtttgt acagcacaat
    15181 tctattttgt gcgggtaagt tgttgcgtca ggaggcgttg tggatttctc aatcatggtt
    15241 tacgcagtta ttgcgttggt gggtgtggca attggctggc tgtttgccag ttatcaacat
    15301 gcgcagcaaa aagccgagca attagctgaa cgtgaagaga tggtcgcgga gttaagcgcg
    15361 gcaaaacaac aaattaccca aagcgagcac tggcgtgcag agtgtgaatt gctcaataac
    15421 gaagtgcgca gcctgcaaag tattaacacc tctctggagg ccgatctgcg tgaagtaacc
    15481 acgcggatgg aagccgcaca gcaacatgct gacgataaaa ttcgccagat gattaacagc
    15541 gagcagcgcc tcagtgagca gtttgaaaac ctcgccaacc gtatttttga gcacagcaat
    15601 cgccgggttg atgagcaaaa ccgtcagagt ctgaacagcc tgttgtcgcc gctacgtgaa
    15661 caactggacg gtttccgccg tcaggttcag gacagcttcg gtaaagaagc acaagaacgc
    15721 cataccctga cccacgaaat tcgcaatctc cagcaactca acgcgcaaat ggcccaggaa
    15781 gcgatcaacc tgacgcgcgc gctgaaaggc gacaataaaa cccagggcaa ctggggcgag
    15841 gtagtattga cgcgggtgct ggaggcttcc ggtctgcgtg aagggtatga atatgaaacc
    15901 caggtcagca tcgaaaatga cgcccgctcg cggatgcagc cggatgtcat cgtgcggctg
    15961 ccgcagggaa aagatgtggt gatcgacgcc aaaatgacgc tggtcgccta tgaacgctat
    16021 tttaacgccg aagacgacta cacccgcgaa agcgcgctac aggaacatat cgcgtcggtg
    16081 cgtaaccata tccgtttgct gggacgcaaa gattatcaac agctgccggg gctgcgaact
    16141 ctggattacg tgctgatgtt tattcccgtt gaacccgctt ttttactggc gcttgaccgc
    16201 cagccggagc tgatcaccga agcgttgaaa aacaatatca tgctggttag cccgactacg
    16261 ctgctggtgg cgttgcgtac tattgccaac ctgtggcgtt atgagcatca aagccgtaac
    16321 gcccagcaaa tcgccgatcg tgccagcaag ctgtacgaca agatgcgttt gttcatcgat
    16381 gacatgtccg cgattggtca aagtctcgac aaagcgcagg ataactaccg ccaggcaatg
    16441 aaaaaactct cttcaggtcg cggaaatgtg ctggcgcagg cagaagcgtt tcgcggttta
    16501 ggagtagaaa ttaaacgcga gattaatccg gatttggctg aacaggcggt gagccaggat
    16561 gaagagtatc gacttcggtc ggttccggag cagccgaatg atgaagctta tcaacgcgat
    16621 gatgaatata atcagcagtc gcgctagccc attaggggta gttaagccgg gtagaaatct
    16681 agggcatcga cgcccaatct gttacacttc tggaacaatt ttttgatgag caggcattga
    16741 gatggtggat aagtcacaag aaacgacgca ctttggtttt cagaccgtcg cgaaggaaca
    16801 aaaagcggat atggtcgccc acgttttcca ttccgtggca tcaaaatacg atgtcatgaa
    16861 tgatttgatg tcatttggta ttcatcgttt gtggaagcga ttcacgattg attgcagcgg
    16921 cgtacgccgt gggcagaccg tgctggatct ggctggtggc accggcgacc tgacagcgaa
    16981 attctcccgc ctggtcggag aaactggcaa agtggtcctt gctgatatca atgaatccat
    17041 gctcaaaatg ggacgcgaga agctgcgtaa tatcggtgtg attggcaacg ttgagtatgt
    17101 tcaggcgaac gctgaggcgc tgccgttccc ggataacacc tttgattgca tcaccatttc
    17161 gtttggtctg cgtaacgtca ccgacaaaga taaagcactg cgttcaatgt atcgcgtgct
    17221 gaaacccggc ggccgcctgc tggtgcttga gttctcgaag ccaattatcg agccgctgag
    17281 caaagcctat gatgcatact ccttccatgt gctgccgcgt attggctcac tggtcgcgaa
    17341 cgacgccgac agctaccgtt atctggcaga atccatccgt atgcatcccg atcaggatac
    17401 cctgaaagcc atgatgcagg atgccggatt cgaaagtgtc gactactaca atctgacggc
    17461 aggggttgtg gcgctgcatc gtggttataa gttctgacag gagaccggaa atgcctttta
    17521 aacctttagt gacggcagga attgaaagtc tgctcaacac cttcctgtat cgctcacccg
    17581 cgctgaaaac agcccgctcg cgtctgctgg gtaaagtatt gcgcgtggag gtaaaaggct
    17641 tttcgacgtc attgattctg gtgttcagcg aacgccaggt tgatgtactg ggcgaatggg
    17701 caggcgatgc tgactgcacc gttatcgcct acgccagtgt gttgccgaaa cttcgcgatc
    17761 gccagcagct taccgcactg attcgcagtg gtgagctgga agtgcagggc gatattcagg
    17821 tggtgcaaaa cttcgttgcg ctggcagatc tggcagagtt cgaccctgcg gaactgctgg
    17881 ccccttatac cggtgatatc gccgctgaag gaatcagcaa agccatgcgc ggaggcgcaa
    17941 agttcctgca tcacggcatt aagcgccagc aacgttatgt ggcggaagcc attactgaag
    18001 agtggcgtat ggcacccggt ccgcttgaag tggcctggtt tgcggaagag acggctgccg
    18061 tcgagcgtgc tgttgatgcc ctgaccaaac ggctggaaaa actggaggct aaatgacgcc
    18121 aggtgaagta cggcgcctat atttcatcat tcgcactttt ttaagctacg gacttgatga
    18181 actgatcccc aaaatgcgta tcaccctgcc gctacggcta tggcgatact cattattctg
    18241 gatgccaaat cggcataaag acaaactttt aggtgagcga ctacgactgg ccctgcaaga
    18301 actggggccg gtttggatca agttcgggca aatgttatca acccgccgcg atctttttcc
    18361 accgcatatt gccgatcagc tggcgttatt gcaggacaaa gttgctccgt ttgatggcaa
    18421 gctggcgaag cagcagattg aagctgcaat gggcggcttg ccggtagaag cgtggtttga
    18481 cgattttgaa atcaagccgc tggcttctgc ttctatcgcc caggttcata ccgcgcgatt
    18541 gaaatcgaat ggtaaagagg tggtgattaa agtcatccgc ccggatattt tgccggtcat
    18601 taaagcggat ctgaaactta tctaccgtct ggctcgctgg gtgccgcgtt tgctgccgga
    18661 tggtcgccgt ctgcgcccaa ccgaagtggt gcgcgagtac gaaaagacct tgattgatga
    18721 actgaatttg ctgcgggaat ctgccaacgc cattcagctt cggcgcaatt ttgaagacag
    18781 cccgatgctc tacatcccgg aagtttaccc tgactattgt agtgaaggga tgatggtgat
    18841 ggagcgcatt tacggcattc cggtgtctga tgttgcggcg ctggagaaaa acggcactaa
    18901 catgaaattg ctggcggaac gcggcgtgca ggtgttcttc actcaggtct ttcgcgacag
    18961 ctttttccat gccgatatgc accctggcaa catcttcgta agctatgaac acccggaaaa
    19021 cccgaaatat atcggcattg attgcgggat tgttggctcg ctaaacaaag aagataaacg
    19081 ctatctggca gaaaacttta tcgccttctt taatcgcgac tatcgcaaag tggcagagct
    19141 acacgtcgat tctggctggg tgccaccaga taccaacgtt gaagagttcg aatttgccat
    19201 tcgtacggtc tgtgaaccta tctttgagaa accgctggcc gaaatttcgt ttggacatgt
    19261 actgttaaat ctgtttaata cggcgcgtcg cttcaatatg gaagtgcagc cgcaactggt
    19321 gttactccag aaaaccctgc tctatgtcga aggggtagga cgccagcttt atccgcagct
    19381 cgatttatgg aaaacagcga agcctttcct ggagtcgtgg attaaagatc aggtcggtat
    19441 tcctgctctg gtgagagcat ttaaagaaaa agcgccgttc tgggtcgaaa aaatgccaga
    19501 actgcctgaa ctggtttacg acagtttgcg ccagggcaag tatttacagc acagtgttga
    19561 taagattgcc cgcgagcttc agtcaaatca tgtacgtcag ggacaatcgc gttattttct
    19621 cggaattggc gctacgttag tattaagtgg cacattcttg ttggtcagcc gacctgagtg
    19681 ggggctgatg cccggctggt taatggcagg tggtctgatc gcctggtttg tcggttggcg
    19741 caaaacacgc tgattttttc atcgctcaag gcgggccgtg taacgtataa tgcggctttg
    19801 tttaatcatc atctaccaca gaggaacatg tatgggtggt atcagtattt ggcagttatt
    19861 gattattgcc gtcatcgttg tactgctttt tggcaccaaa aagctcggct ccatcggttc
    19921 cgatcttggt gcgtcgatca aaggctttaa aaaagcaatg agcgatgatg aaccaaagca
    19981 ggataaaacc agtcaggatg ctgattttac tgcgaaaact atcgccgata agcaggcgga
    20041 tacgaatcag gaacaggcta aaacagaaga cgcgaagcgc cacgataaag agcaggtgta
    20101 atccgtgttt gatatcggtt ttagcgaact gctattggtg ttcatcatcg gcctcgtcgt
    20161 tctggggccg caacgactgc ctgtggcggt aaaaacggta gcgggctgga ttcgcgcgtt
    20221 gcgttcactg gcgacaacgg tgcagaacga actgacccag gagttaaaac tccaggagtt
    20281 tcaggacagt ctgaaaaagg ttgaaaaggc gagcctcact aacctgacgc ccgaactgaa
    20341 agcgtcgatg gatgaactac gccaggccgc ggagtcgatg aagcgttcct acgttgcaaa
    20401 cgatcctgaa aaggcgagcg atgaagcgca caccatccat aacccggtgg tgaaagataa
    20461 tgaagctgcg catgagggcg taacgcctgc cgctgcacaa acgcaggcca gttcgccgga
    20521 acagaagcca gaaaccacgc cagagccggt ggtaaaacct gctgcggacg ctgaaccgaa
    20581 aaccgctgca ccttcccctt cgtcgagtga taaaccgtaa acatgtctgt agaagatact
    20641 caaccgctta tcacgcatct gattgagctg cgtaagcgtc tgctgaactg cattatcgcg
    20701 gtgatcgtga tattcctgtg tctggtctat ttcgccaatg acatctatca cttggtatcc
    20761 gcgccattga tcaagcagtt gccgcaaggt tcaacgatga tcgccaccga cgtggcctcg
    20821 ccgttcttta cgccgatcaa gctgaccttt atggtgtcgc tgattctgtc agcgccggtg
    20881 attctctatc aggtgtgggc atttatcgcc ccagcgctgt ataagcatga acgtcgcctg
    20941 gtggtgccgc tgctggtttc cagctctctg ctgttttata tcggcatggc attcgcctac
    21001 tttgtggtct ttccgctggc atttggcttc cttgccaata ccgcgccgga aggggtgcag
    21061 gtatccaccg acatcgccag ctatttaagc ttcgttatgg cgctgtttat ggcgtttggt
    21121 gtctcctttg aagtgccggt ggcaattgtg ctgctgtgct ggatggggat tacctcgtca
    21181 gaagacttac gcaaaaaacg cccgtatgtg ctggttggtg cattcgttgt cgggatgttg
    21241 ctgacgccgc cggatgtctt ctcgcaaacg ctattggcga tcccgatgta ctgtctgttt
    21301 gaaatcggtg tcttcttctc acgcttttac gttggtaaag ggcgaaatcg ggaagaggaa
    21361 aacgacgctg aagcagaaag cgaaaaaact gaagaataaa ttcaaccgcc cgtcagggcg
    21421 gttgtcatat ggagtacagg atgtttgata tcggcgttaa tttgaccagt tcgcaatttg
    21481 cgaaagaccg tgatgatgtt gtagcgcgcg cttttgacgc gggagttaat gggctactca
    21541 tcaccggcac taacctgcgt gaaagccagc aggcgcaaaa gctggcgcgt cagtattcgt
    21601 cctgttggtc aacggcgggc gtacatcctc acgacagcag ccagtggcaa gctgcgactg
    21661 aagaagcgat tattgagctg gccgcgcagc cagaagtggt ggcgattggt gaatgtggtc
    21721 tcgactttaa ccgtaacttt tcgacgccgg aagagcagga acgcgctttt gttgctcagc
    21781 tacgcattgc cgcagaatta aacatgccgg tatttatgca ctgtcgcgat gcccacgagc
    21841 ggtttatgac attgctggag ccgtggctgg ataaactgcc tggtgcggtt cttcattgct
    21901 ttaccggcac acgcgaagag atgcaggcgt gcgtggcgcg tggaatttat atcggcatta
    21961 ccggttgggt ttgcgatgaa cgacgcgggc tggagctgcg ggaattgttg ccgttgattc
    22021 cggcggagaa attgctgatc gaaacagatg cgccgtatct gctcccgcgc gatctcacgc
    22081 caaagccatc atcccggcgc aacgagccag cccatctgcc ccatattttg caacgtattg
    22141 cgcactggcg tggagaagat gccgcatggc tggctgccac cacggatgcc aatgtcaaaa
    22201 cactgtttgg gattgcgttt tagagtttgc ggaactcggt gttcttcaca ctgtgcttaa
    22261 tctctttatt aataagatta agcaatagca tggagcgagc ctcaccatcg ggttcggtga
    22321 aaatggcctg aaagccttcg aacgcgcctt cggtaataat caccttatca cccggataag
    22381 ggggggccgg atcgacaatg tctttcggtt tatataccga tagctgatga attaccgccg
    22441 atgggactat cgctggcgac gcgccaaagc gcacaaagtg gctgacgcca cgcgtagcgc
    22501 tgatggtcgt ggtatgaatc acttctggat caaattccac aaacaggtag ttagggaata
    22561 acggttcact gactgcggta cgtttacccc gtacgatttt ttccagggtg atcatcggag
    22621 cgaggcaatt aaccgcctgt ctctcaaggt gttcctgggc acgttgaagt tgcccgcgct
    22681 tgcagtacag taaataccag gattgcataa tgactcttat ccgtttaatc ggggcgcaag
    22741 gatagcaaaa gctttacgct aagttaatta tattccccgg tttgcgttat accgtcagag
    22801 ttcacgctaa tttaacaaat ttacagcatt gcaaagatga acgccgtata atgggcgcag
    22861 attaagaggc tacaatggac gccatgaaat ataacgattt acgcgacttc ctgacgctgc
    22921 ttgaacagca gggtgagcta aaacgtatca cgctcccggt ggatccgcat ctggaaatca
    22981 ctgaaattgc tgaccgcact ctgcgtgctg gtgggcctgc gctgttgttc gaaaacccta
    23041 aaggctactc aatgccggtg ctgtgcaacc tgttcggtac gccaaagcgc gtggcgatgg
    23101 gcatggggca ggaagatgtt tcggcgctgc gtgaagttgg taaattattg gcgtttctga
    23161 aagagccgga gccgccaaaa ggtttccgcg acctgtttga taaactgccg cagtttaagc
    23221 aggtgttaaa catgccgaca aagcgactgc gtggtgcgcc ctgccaacaa aaaatcgtct
    23281 ctggcgatga cgtcgatctc aatcgcattc ccattatgac ctgctggccg gaagatgccg
    23341 cgccgctgat tacctggggg ctgaccgtaa cgcgcggccc gcataaagag cggcagaatc
    23401 tgggcattta tcgccagcag ctgattggta aaaacaaact gattatgcgc tggctgtcgc
    23461 atcgcggcgg cgcgctggat tatcaggagt ggtgtgcggc gcatccgggc gaacgtttcc
    23521 cggtttctgt ggcgctgggt gccgatcctg ccacgattct cggtgcagtc acccccgttc
    23581 cggatacgct ttcagagtat gcgtttgccg gattgctacg cggcaccaaa accgaagtag
    23641 taaagtgtat ttccaatgac ctcgaagtgc cagccagtgc cgaaatcgtc ctggaagggt
    23701 atatcgatcc tggtgagatg gcgccggaag ggccgtatgg tgaccacaca ggttactata
    23761 atgaagtcga taatttcccg gtgtttaccg tgacgcatat tacccagcgt gaagatgcga
    23821 tttaccattc cacctatacc gggcgtccgc cagatgagcc cgcggtactg ggcgtggcgt
    23881 tgaacgaagt gtttgtaccg attctgcaaa aacagttccc ggaaattgtc gatttttacc
    23941 tgccgccgga aggctgttct tatcgtctgg cggtagtgac gatcaaaaaa cagtacgccg
    24001 gacacgcgaa gcgcgtcatg atgggcgtct ggtcgttctt acgccagttt atgtacacta
    24061 aatttgtgat cgtttgcgat gatgacgtca acgcacgcga ctggaacgat gtgatttggg
    24121 cgattaccac ccgtatggac ccggcgcggg atactgttct ggtagaaaat acgcctattg
    24181 attatctgga ttttgcctcg cctgtctctg ggctgggttc aaaaatgggg ctggatgcca
    24241 cgaataaatg gccgggggaa acccagcgtg aatggggacg tcccatcaaa aaagatccag
    24301 atgttgtcgc gcatattgac gccatctggg atgaactggc tatttttaac aacggtaaaa
    24361 gcgcctgatg cgcgtttgtt ttgccctatt tatcgatccg acagagaaag cgcatgacaa
    24421 ccttaagctg taaagtgacc tcggtagcag ctatcacgga taccgtatat cgtgtccgca
    24481 tcgtgccaga cgcggccttt tcttttcgtg ctggtcagta tttgatggta gtgatggatg
    24541 agcgcgacaa acgtccgttc tcaatggcct cgacgccgga tgaaaaaggg tttatcgagc
    24601 tgcatattgg cgcttctgaa atcaaccttt acgcgaaagc agtcatggac cgcatcctca
    24661 aagatcatca aatcgtggtc gaccttcccc acggagaagc gtggctgcgc gatgatgaag
    24721 agcgtccgat gattttgatt gcgggcggca ccgggttctc ttatgcccgc tcgattttgc
    24781 tgacagcgtt ggcgcgtaac ccaaaccgtg atatcaccat ttactggggc gggcgtgaag
    24841 agcagcatct gtatgatctc tgcgagcttg aggcgctttc gttaaagcac cttggtctgc
    24901 aagtggtgcc ggtggttgaa caaccggaag caggctggcg tgggcgtact ggtaccgtgt
    24961 taacggcggt attgcaggat catggtacgc tggcagagca tgatatctat attgccggac
    25021 gttttgagat ggcgaaaatt gcccgtgacc tgttttgcag tgagcgtaat gcgcgggaag
    25081 atcgcctgtt tggcgatgcg tttgcattta tctgagatat aaaaaaaccc gcccctgaca
    25141 ggcgggaaga acggcaacta aaactttatt cagtggcatt tagatctatg tcgcatccgg
    25201 caattggtgc acgatgcctg atgcgacgct ggcgcgtctt atcaggccta cattggtgcc
    25261 ggatcggtag accggataag gcgttcacgc cgcatccggc aagtggttaa acccgctcaa
    25321 acaccgtcgc aataccctga cccagaccga tacacatcgt cgccagacca aactgaacgt
    25381 ctttgcgttc catcagattc agcagcgtgg tgctgatacg cgcaccggaa caacccagcg
    25441 gatgacccag cgcgatcgca ccaccattga ggttgatctt ctcgtcaatc tgctccatta
    25501 gcccgagatc tttaatgcac ggcaggatct gcgcggcaaa ggcttcgttc atctcaaaca
    25561 cgccgatatc gctggcagaa agccccgctt ttttcagcgc cagcttcgag gccggaaccg
    25621 ggccgtaacc cataatcgat gggtcacaac caacgaccgc catcgaacgc acacgagcgc
    25681 gcggcttaag acctaattca tgggcgcggc tttcactcat caccagcatg gcagctgcgc
    25741 catcggaaag tgcagaagat gtgcccgccg ttaccgtacc gtttactgga tcaaacgccg
    25801 gacgcagcgt ggcgagggct tccacggtgg tttccgggcg aatcacttcg tcgtaattaa
    25861 actgcttcag gacgccgtcg gcatcgtgac caccagttgg gatgatttca tttttaaatg
    25921 cggccgactg cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag gcatcctgca
    25981 tttcacggct gataccgtgc atacgcgcca gcatttctgc cgttaagccc atcatgcccg
    26041 ccgctttggc gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtga ctcatcggca
    26101 catggcctat atgctccacg ccgccaacca gacatgcctg cgtatcgcca gtcatgatca
    26161 ttcgtgctgc gtcatgcagt gcctgcatgg atgaaccaca caagcgattg acggtaaccg
    26221 ccgggacaga gtgcggcact tctgccagca gcgccgcgtt acgggcgatg ttaaaaccct
    26281 gctccagcgt ctgttgcaca caaccccagt aaatatcgtc gagggccgcc gcttccagcg
    26341 ccgggttacg cgccagcagg ctacgcatta aatgagcgga gagatcttcc gcacgcacgt
    26401 tacgaaaagc accgcccttc gaacggccca tcggggtgcg aattgcatcg acaatgacaa
    26461 cctgttccat tgtgactcct taagccgttt tcaggtcgcc aaccggacgg gctggctcaa
    26521 ccggaggata gtacggttcg ttatgacgcg ctttattacg cagaccttcc ggcacttcat
    26581 acagcgggcc gaggtgctga tattgctgtg ccatatcgag gtattttgcg ctaccgaggg
    26641 tgtccagcca gcggaacgcg ccgccgtgga acggagggaa gcccaggccg tagaccagcg
    26701 ccatatccgc ttccgccgga gtggcgataa tgccctcttc cagacagcgc accacttcgt
    26761 tgaccatcgg gatcatcatg cgggcgataa tctcttcttc gctgaaatcg cgcttcggtt
    26821 ggctcacttc tgccagcagg tcttcaacgg cggcgtcttc ttctttcttc ggcttacctt
    26881 tgctgtcttc tttataacgc cagaaaccga ggccgttctt ctgaccaaag cggttggcat
    26941 caaacagcgc gtcgatggca tcgcggtaat ctttctgcat ccgctgcggg aagcctgctg
    27001 ccatgacagc ctgagcgtga tgcgcggtat caatgcccac aacgtccagc agatatgccg
    27061 ggcccatcgg ccagccaaac tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc
    27121 cgtcgcgcag cagctggctg aaaccggcga aatacgggaa cagcacgcgg ttaacaaaga
    27181 agccggggca gtcgttaacc acaatcggcg tcttgcccat cttgctcgcc caggcgacaa
    27241 ctttcgcgat ggtttcgtcg gagcttttct cgccgcgaat aatttctacc aacggcattc
    27301 ggtggaccgg gttaaagaag tgcatcccgc agaagttttc cgggcgttcc agcgcgttgg
    27361 ccagttcgct gataggaatg gttgaagtgt tagacgccag cacggtatcc tggcgtactt
    27421 tttgttcggt ttctgccagt acggcttttt tcactttcgg gttttcaaca accgcttcta
    27481 ccacaatatc cacgcggtca aatccggcgt agtcgagcgt tgggtggatc gtggagatca
    27541 cgccagccag tttcagacca tcgattttgc cgcgctcaag ctgcttgttc agcagtttcg
    27601 cggcttcggt catgccgagg gttaacgact tgtcgttgat atctttcatg acaaccggca
    27661 cgcctttcca cgcggactgg taagcgatac cgccacccat gatgcctgcg cccagcacag
    27721 cagcctgttt cggggtttca acgtctttgg tgagtttctt cgctttgcct tttacatatt
    27781 gatcgttaag gaaaatgccg accagtgcgc gagcttcgtt ggtatgcgcc agcgggacaa
    27841 aacttttgtt ttccagattt aaggcttctt cacgaccaaa acgggccgca gcttcaatgg
    27901 tttttactgc ggtgatgggg gccggataat gtttccccgc cgtttgcgcg accattcctt
    27961 tagcgatggt gaagctcatg gtggcttcaa tcttgctcag ttttagcggt tcctgcttcg
    28021 gctgacgttt cgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa atcgccatcg
    28081 ccccttcaat cagtttttct gctttgacta cgccatccac cagaccgatt ttcagcgcct
    28141 gatccgcgcc gacatcttta ccggcggcaa tgatttccag tgcactgtca gcgcccagca
    28201 tacgtggcat acgtacagaa ccgccaaagc ccggcatgat gcccagtttg gtttccggca
    28261 gaccgatgcg cagatccggc gtcgccagac gataatcggt cgccagcacg cattcgcagc
    28321 caccacccag cgcatagccg ttgacggcag caatggtcgg caccggcaga tcttccaggc
    28381 gattaaacac gctattggca aagtgcagcc actgacttaa ctgttcttca ggaacgagga
    28441 acagggacaa aaattcggtg atatcagcac cgacgataaa ggctgctttg ttcgaacgca
    28501 gcagcagccc ttttagatct gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg
    28561 cgacggtcgc agtgtcgagt ttattaactg aacctggggc atcaaatacc agttcggcaa
    28621 tgccatcttc cagccagtca aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct
    28681 gaatccgcaa ggtaatctgg tcgtaccaga tgcatcggag tgtgtgtttt gtgttaaaaa
    28741 tatgcaaata aaagattaaa gaaatgccga tctgatcacg ctcggcagaa atcacgctct
    28801 ggatgaacga tgtgctaaga tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat
    28861 ggaatcactg gcctcgctct ataaaaatca tatagctacc ttacaagaac ggactcgcga
    28921 tgcgctggcg cgcttcaagc tggatgcatt acttattcac tccggcgagc tgttcaacgt
    28981 ttttctcgac gatcatccct atccgtttaa agtgaacccg caattcaaag cgtgggtgcc
    29041 ggtaactcag gtgccaaact gctggttgct ggtggatggc gtgaacaagc cgaaactgtg
    29101 gttctatctg ccggttgatt actggcacaa cgtcgaaccg ctgccgacct ccttctggac
    29161 tgaagatgtg gaagtgatcg cgctgccgaa agccgatggc attggtagcc tgctgcctgc
    29221 tgcgcgcggc aatatcggtt atatcggtcc ggtgccggaa cgtgcgctgc aactgggtat
    29281 tgaggccagc aacatcaacc cgaaaggggt gatcgactac ctgcattatt accgctcctt
    29341 caaaaccgag tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg tcaatggtca
    29401 tcgtgcggca gaagaagcgt tccgttctgg catgagcgag ttcgatatca atattgccta
    29461 tctgaccgcg accggtcatc gtgataccga cgtaccttac agcaacattg tggcgcttaa
    29521 cgaacacgct gcggtgttgc attacaccaa actggaccac caggcaccgg aagagatgcg
    29581 cagcttcctg ctggatgccg gggccgaata taacggctat gcggctgacc tgacccgtac
    29641 ctggtcggca aaaagcgaca acgactacgc acagctggtg aaagacgtaa atgatgaaca
    29701 actggcgctg atcgccacca tgaaagcagg cgtcagctat gtggattacc acatccagtt
    29761 ccatcagcgc atcgccaaac tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga
    29821 agcgatggtc gaaaacgatc tcaccggacc gtttatgccg cacggtattg gccatccgct
    29881 gggcctgcag gtgcatgacg tcgccggttt tatgcaggat gatagcggta cacacctcgc
    29941 ggcaccggca aaatatccgt acctgcgcta cacccgtatt ctccagccgg gcatggtgtt
    30001 aaccatcgaa ccgggtatct acttcattga atcgcttctg gcaccgtggc gtgaagggca
    30061 gttcagcaag cacttcaact ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg
    30121 tatcgaagac aacgtggtga tccacgaaaa caacgtggaa aacatgaccc gggatctgaa
    30181 actggcgtga tggaaagctg gttaattcct gcggcaccgg tcacggtcgt tgaagagatc
    30241 aaaaagagcc gcttcataac gatgttggcg cataccgatg gcgttgaggc ggcgaaagcg
    30301 tttgttgaat cggtgcgggc agaacacccc gatgcccgcc accattgcgt ggcgtgggtg
    30361 gcgggtgcgc cggatgattc tcaacagctg ggtttctctg acgacgggga gccggcggga
    30421 acggcaggta aaccgatgct cgcccagcta atgggcagcg gcgtcgggga aattaccgct
    30481 gtggtagtgc gctactacgg cggcatattg cttggcaccg gtgggttagt gaaagcgtat
    30541 ggcggcggcg tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac gccattaacc
    30601 gaatatactt tgcaatgtga atatagtcag ttaaccggca ttgaagcgtt gctggggcag
    30661 tgtgacggca aaattatcaa cagtgattat caggcattcg ttctgctgcg ggtggcgctt
    30721 ccggcggcga aagtggctga attttccgca aagctggcgg attttagccg tggttcattg
    30781 caattgttag cgattgaaga ataatcccca cttcgttttg cagaactaag gaagcggcag
    30841 agatgcattt tcgcgccatt acccgaatcg ttggactact ggtcatctta ttttcaggga
    30901 ccatgattat ccctgggctg gtagcactca tctaccggga tggagcgggc cgcgctttta
    30961 cccagacctt ttttgtcgcc ctcgccattg gctctatgct gtggtggccg aaccgcaaag
    31021 agaagggcga actgaaatcc cgtgaggggt ttctgatagt ggtgctgttt tggaccgtgc
    31081 tgggtagtgt cggtgcgctc ccttttatct tctcggaaag cccgaacctc acgattaccg
    31141 atgcgttttt tgaatctttc tctggcctga ccaccaccgg ggccactacg ctggtggggc
    31201 tggattcgct ccctcatgcc atcctctttt atcgccagat gctgcaatgg tttggcggga
    31261 tggggatcat cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt gggatgcagc
    31321 tctatcgcgc agaaatgccc ggcccgctga aagataacaa aatgcgcccg cgaattgcgg
    31381 aaacggcgaa aactctgtgg ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt
    31441 tgtggtttgc cggaatggat gcctttgatg ccatcggcca tagctttgcg actatcgcta
    31501 ttggcggctt ctcgacacat gatgccagta tcggttattt cgacagtccg actattaaca
    31561 ctatcattgc tatcttcctg ctgatctccg gctgtaacta cggtctgcac ttttcactgt
    31621 taagtgggcg tagtctgaag gtttattggc gcgatccgga atttcgcatg tttatcggcg
    31681 tacagtttac gctggtggtt atttgtacac tcgtactgtg gtttcataat gtctacagtt
    31741 cggcgctgat gacaattaac caggcgtttt tccaggtggt atcgatggcg acaaccgccg
    31801 ggtttacgac tgacagcatt gcccgctggc cgctcttttt gccggtactg cttttatgtt
    31861 cagcttttat cggcggttgc gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc
    31921 tgctgctgtt taagcagggg aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct
    31981 atagcattaa gctggggaat cgcgcactgc cggaacgtat cctcgaagcc gtttggggat
    32041 ttttctccgc ctatgcattg gtgtttattg tcagtatgct ggcgattatc gccacgggcg
    32101 tggatgattt ttctgccttt gcctcagttg ttgcgacatt gaataacctg gggccagggc
    32161 ttggcgtggt tgctgataac tttaccagta tgaacccggt ggctaaatgg atcctgattg
    32221 ccaacatgct gtttggtcgt ctcgaggtct ttacattact ggtgctcttt accccgactt
    32281 tctggcgtga atgatggagt aatacgtgaa aacattaatt cttttctcaa caagggacgg
    32341 acaaacgcgc gagattgcct cctacctggc ttcggaactg aaagaactgg ggatccagac
    32401 ggatgtcgcc aatgtgcacc gcattgaaga accacaatgg gaaaactatg accgtgtggt
    32461 cattggtgct tctattcgct atggtcacta ccactctgcg ttccaggaat ttgtcaaaaa
    32521 acatgcgacg cgactgaatt cgatgccgag cgccttttat tccgtaaacc tggtggcgcg
    32581 caaaccggag aagcgtactc cacagaccaa cagctacgcg cggaagtttc tgatgaactc
    32641 gcaatggcgt cccgatcgct gcgcggtcat tgccggggcg ctgcgttacc cacgttatcg
    32701 ctggtacgac cgttttatgc tcaagctgat tatgaagatg tcaggcggtg aaacggatac
    32761 gcgcaaagaa gttgtctata ccgattggga gcaggtggcg aatttcgccc gagaaatcgc
    32821 ccatttaacc gacaaaccga cgctgaaata agcataaaga ataaaaaatg cgcggtcaga
    32881 aaattatttt aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg
    32941 acacggaaca acggcaaaca cgcagccggg tcagcggggt tctcctgaga attccgacag
    33001 agaaagcaaa aataaatgct tgactttgta gcgggaaagc gtattatgaa caccccgcgc
//
LOCUS       DAJTYM010000039        31726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTYM010000039 DAJTYM010000000
VERSION     DAJTYM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(26..451)
                     /gene="exbD"
                     /locus_tag="OO575_001859"
     CDS             complement(26..451)
                     /gene="exbD"
                     /locus_tag="OO575_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6664850.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(458..1192)
                     /gene="exbB"
                     /locus_tag="OO575_001860"
     CDS             complement(458..1192)
                     /gene="exbB"
                     /locus_tag="OO575_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6664851.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            1444..2631
                     /gene="metC"
                     /locus_tag="OO575_001861"
     CDS             1444..2631
                     /gene="metC"
                     /locus_tag="OO575_001861"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6664852.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            2771..3430
                     /locus_tag="OO575_001862"
     CDS             2771..3430
                     /locus_tag="OO575_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6664853.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(3470..4369)
                     /gene="yqhC"
                     /locus_tag="OO575_001863"
     CDS             complement(3470..4369)
                     /gene="yqhC"
                     /locus_tag="OO575_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6664854.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            4563..5726
                     /gene="yqhD"
                     /locus_tag="OO575_001864"
     CDS             4563..5726
                     /gene="yqhD"
                     /locus_tag="OO575_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6664855.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            5831..6658
                     /gene="dkgA"
                     /locus_tag="OO575_001865"
     CDS             5831..6658
                     /gene="dkgA"
                     /locus_tag="OO575_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6664856.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            6858..7784
                     /locus_tag="OO575_001866"
     CDS             6858..7784
                     /locus_tag="OO575_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6664857.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            7835..8092
                     /gene="yqhH"
                     /locus_tag="OO575_001867"
     CDS             7835..8092
                     /gene="yqhH"
                     /locus_tag="OO575_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6664858.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(8135..10354)
                     /locus_tag="OO575_001868"
     CDS             complement(8135..10354)
                     /locus_tag="OO575_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6664859.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(10465..11877)
                     /gene="ftsP"
                     /locus_tag="OO575_001869"
     CDS             complement(10465..11877)
                     /gene="ftsP"
                     /locus_tag="OO575_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6664860.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(11952..12689)
                     /gene="plsC"
                     /locus_tag="OO575_001870"
                     /gene_synonym="parF"
     CDS             complement(11952..12689)
                     /gene="plsC"
                     /locus_tag="OO575_001870"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6664861.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(12923..15181)
                     /gene="parC"
                     /locus_tag="OO575_001871"
     CDS             complement(12923..15181)
                     /gene="parC"
                     /locus_tag="OO575_001871"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6664862.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(15727..16209)
                     /locus_tag="OO575_001872"
     CDS             complement(15727..16209)
                     /locus_tag="OO575_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6664863.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(16262..16654)
                     /gene="ygiW"
                     /locus_tag="OO575_001873"
     CDS             complement(16262..16654)
                     /gene="ygiW"
                     /locus_tag="OO575_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6664864.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            16806..17465
                     /gene="qseB"
                     /locus_tag="OO575_001874"
     CDS             16806..17465
                     /gene="qseB"
                     /locus_tag="OO575_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6664865.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            17462..18736
                     /gene="qseC"
                     /locus_tag="OO575_001875"
     CDS             17462..18736
                     /gene="qseC"
                     /locus_tag="OO575_001875"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6664866.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(18782..19114)
                     /locus_tag="OO575_001876"
     CDS             complement(18782..19114)
                     /locus_tag="OO575_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6664867.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            19433..20014
                     /gene="mdaB"
                     /locus_tag="OO575_001877"
     CDS             19433..20014
                     /gene="mdaB"
                     /locus_tag="OO575_001877"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6664868.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            20045..20359
                     /locus_tag="OO575_001878"
     CDS             20045..20359
                     /locus_tag="OO575_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6664869.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(20407..22299)
                     /gene="parE"
                     /locus_tag="OO575_001879"
     CDS             complement(20407..22299)
                     /gene="parE"
                     /locus_tag="OO575_001879"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6664870.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(22328..22909)
                     /gene="yqiA"
                     /locus_tag="OO575_001880"
     CDS             complement(22328..22909)
                     /gene="yqiA"
                     /locus_tag="OO575_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6664871.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(22909..23736)
                     /gene="cpdA"
                     /locus_tag="OO575_001881"
     CDS             complement(22909..23736)
                     /gene="cpdA"
                     /locus_tag="OO575_001881"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6664872.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(23761..24183)
                     /locus_tag="OO575_001882"
     CDS             complement(23761..24183)
                     /locus_tag="OO575_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6664873.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(24184..24813)
                     /gene="nudF"
                     /locus_tag="OO575_001883"
     CDS             complement(24184..24813)
                     /gene="nudF"
                     /locus_tag="OO575_001883"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6664874.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            25018..26499
                     /gene="tolC"
                     /locus_tag="OO575_001884"
     CDS             25018..26499
                     /gene="tolC"
                     /locus_tag="OO575_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6664875.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            26647..27318
                     /locus_tag="OO575_001885"
     CDS             26647..27318
                     /locus_tag="OO575_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6664876.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            27324..28484
                     /locus_tag="OO575_001886"
     CDS             27324..28484
                     /locus_tag="OO575_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6664877.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDTGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(28522..29310)
                     /gene="ygiD"
                     /locus_tag="OO575_001887"
     CDS             complement(28522..29310)
                     /gene="ygiD"
                     /locus_tag="OO575_001887"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6664878.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            29453..30226
                     /gene="zupT"
                     /locus_tag="OO575_001888"
     CDS             29453..30226
                     /gene="zupT"
                     /locus_tag="OO575_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6664879.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(30284..30454)
                     /locus_tag="OO575_001889"
     CDS             complement(30284..30454)
                     /locus_tag="OO575_001889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664880.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREEAAEKVE"
     gene            complement(30715..31368)
                     /gene="ribB"
                     /locus_tag="OO575_001890"
     CDS             complement(30715..31368)
                     /gene="ribB"
                     /locus_tag="OO575_001890"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6664881.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(31472..31620)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         7895 a         8428 c         7884 g         7519 t
ORIGIN      
        1 gcgtagcgca tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac
       61 ctatcttcag gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat
      121 cgacggtttt gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat
      181 tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt
      241 ctgccttcac cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag
      301 caggcaagtt caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca
      361 gcagaaccaa catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat
      421 cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta tcctgcgcgt aatttttgtg
      481 cgacacgaac cggatgcgca gcggcgctgg cttccagatc taggtcacgg ctttgcagca
      541 acaatacctg cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa
      601 atacgttata gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca
      661 gagcttctgc gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac
      721 caataaagct gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg
      781 cgccgatggt tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc
      841 gacgctccag acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg
      901 acagttccag ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa
      961 aatcagcggc gatatcgttg gcctggttta aggaacgtgc ttcagccagc agttgctgct
     1021 cgcgcttaag gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc
     1081 aggtgactac agaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt
     1141 actgatacat accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc
     1201 tccaggacgc aagtcacaaa atctgcgcat aataatatca aaacgacgtc gaattgatag
     1261 tcgttctcat tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc
     1321 agtaaaaaag tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag
     1381 gatgcgcccg cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc
     1441 gacatggcgg acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac
     1501 actcttggcg cggtaaatag cgtgattcag cgcgcttctt cgctggtatt tgagagtatg
     1561 gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc
     1621 ggaacgttaa cccatttttc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc
     1681 tgcgcgctat ttccctgcgg ggcggcagcg gttgctaatt cgattcttgc ttttgtcgaa
     1741 cagggcgatc atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc
     1801 aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat
     1861 atcgttaagc atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc gggttccatc
     1921 actatggaag tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtgccggat
     1981 gccatcatta tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt
     2041 ggcatcgatg tttctattca ggccgccacc aaatatctgg ttgggcattc agatgcgatg
     2101 attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa tgcctatctg
     2161 atgggccaga tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta
     2221 ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag tggccgaatg gctggcagaa
     2281 catccgcaag ttgcgcgagt taaccaccct gccctgcctg gcagtaaagg acacgaattc
     2341 tggaaacgag actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc
     2401 aatgatgaag agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg
     2461 tggggcgggt atgaatcgtt gatcctggca aatcaaccag aacatatcgc cgccattcgc
     2521 ccacaaggcg agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat
     2581 gtcgacgatc tgattgccga tctggacgcc ggttttgcac ggattgtata acattgccac
     2641 ttttggacaa ttttgcagac attttattgt gaaaagtctt aaattgttgc gtccgggatc
     2701 aaggcgtccc ggacgattca ggagtacaat aggcagataa aggcttaaac gctgttccac
     2761 aggaaagtcc atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc
     2821 cgcgctggcg gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt
     2881 tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc
     2941 aggcgcattg attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc
     3001 cgcagcaagt ctgggctgct ggctaagtta tattcagggg agctggttag ggaataccaa
     3061 aacggtgaaa ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat
     3121 gtttgaccgc cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac
     3181 gctgctgcca accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa
     3241 ctggttaagt ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat
     3301 gattccgttc gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat
     3361 tgccttgtta accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg
     3421 taacgcctga cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct
     3481 gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact
     3541 cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca
     3601 tgccgtcatg gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca
     3661 acggcgaggt gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg
     3721 cttctgccgc cagttgctcg acgctcaggt tttcggtgta tttattctca attcgtttca
     3781 gcacgcggct aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc
     3841 cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac
     3901 gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt
     3961 ctgataacgt ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt
     4021 cgccaatgtc catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg
     4081 ccagcggcac ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca
     4141 gcaggtattc attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc
     4201 cggaaaagag aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct
     4261 cgccatacaa caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca
     4321 gtttattaac tttatccgcc agcaagcgac aaatctcttc acgtttcata tcgcgtaatt
     4381 tcttaggaat aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct
     4441 ggagaaatag gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac
     4501 aatgaaaaac atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag
     4561 taatgaacaa ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg
     4621 ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca
     4681 gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc
     4741 tggaatttgg cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac
     4801 tggttcgcga acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg
     4861 gcaccaaatt tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc
     4921 tgcaaacggg cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc
     4981 cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca
     5041 agcaggcgtt ccattctgca catgttcagc cggtatttgc cgtgctcgat ccggtttata
     5101 cctacaccct gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg
     5161 tggaacagta tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca
     5221 ttttgctgac gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg
     5281 tgcgcgccaa cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcactggcg
     5341 taccgcagga ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg
     5401 atcacgcgca aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca
     5461 agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgacg
     5521 atgagcgtat tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc
     5581 cgacccacct ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac
     5641 tggaagagca cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc
     5701 gccgtatata cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat
     5761 ttcgtccaga cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa
     5821 ggaggaacgt atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca
     5881 gctgggactg ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc
     5941 gttagaagtg ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt
     6001 cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct
     6061 gtggaacgac gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca
     6121 gcttgattat atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt
     6181 cgaagcatgg aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt
     6241 gtgcaacttc cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt
     6301 gataaaccag atcgaacttc atccgctgat gcaacaacgc cagctacacg cttggaacgc
     6361 gacacacaaa atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt
     6421 cgatcagaaa gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt
     6481 tatccgctgg catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg
     6541 tattgccgaa aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcaaaat
     6601 tgcaaaactc gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaacg
     6661 tgcaaattct cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc
     6721 ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc
     6781 atcatgcaga gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac
     6841 ggagaggtga aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta
     6901 ccgtacacgc gcagcctcct tcacagaccg tagaacaaac agtccggcag atttatcaga
     6961 actataaatc agatgccagt acgccttatt tcggtgaaac cggagagcgg gcgataactt
     7021 cagcgcgtat tcaacaggcg cttaccctga acgacaatct tacactaccg ggcaatattg
     7081 gctggctgga ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgttagaaa
     7141 gcgtagcgat aacccaaact gacgtcgatc atgccgatgc cgttgtgcgc tttcgtatct
     7201 ttaaagatga taaagaaaag accacacaga cactgaaaat ggtggcggaa aatggtcgtt
     7261 gggtcattga tgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga
     7321 atgaaaaaac gctggccgct ttagcttcgt tgcagaaaga acagccagaa tcctttgttg
     7381 ctgaactctt tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact
     7441 cttaccgcca ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg
     7501 atgaagatat gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag
     7561 agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc
     7621 gcattcatgt tcgttttacg ctgaccaatg gcaacaatga agagcaagag ctggttttac
     7681 agcggcgcga aggtaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac
     7741 ttaagcagat tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca
     7801 attagccgga acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc
     7861 tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcaact
     7921 ggcgagcaat gtacaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc
     7981 actacgccca caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga
     8041 tgcacaggac tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa
     8101 tccccggcag catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc
     8161 gtttcttcgc accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag
     8221 caggcgtctg acgctgagtc gccatcggcg tatgtttagt caacgccgga cgggtattgc
     8281 ggttctggcg acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc
     8341 gacggctgcc aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc
     8401 agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct
     8461 tcggtacgaa gacgccttcg ctcttataac caatcttcgc cagcggtttt ttcccggtgt
     8521 aatacatggt ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac
     8581 ggaaacgatg ctttttcagc cacagcgcca aattcaccat atcttcatca cgcgtaccgg
     8641 ggtgcgcaga gatgaaatac gggatcagat actgctcttt acctgcctgt ttcgagtagg
     8701 tatcgaacag ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgaca
     8761 acggcccttc ttcggtatgt tccggagcaa tcttcagata accgccgacg tgatgggtcg
     8821 ccaactcttt gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga
     8881 taaggatttt tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt
     8941 cgtggttagt gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag
     9001 tttgttcagc gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga
     9061 gatcggaaat cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa
     9121 tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga
     9181 aagaacagcc gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag
     9241 ccggaatacg ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa
     9301 cgctgtccat ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt
     9361 caccgtgttt ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc
     9421 gcgaagcatg ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaata
     9481 cgtaggtttt ttcccacggt ttctggcgcg gtggctgcac gattacagct ttcgcttcct
     9541 gctttttcgg cgcaacaggt ttgttatccg cacacggcaa atcttcgcca tacggatgcg
     9601 ggattgggtc gattttccct agggtatcaa gacgggtgga atccacaccg ctccagccag
     9661 gcagcgcctc ttttacgata attgcggtat tacgcacatc gcggatttca ctaatcggct
     9721 cgcccatcgc cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca
     9781 gcatgtcggc tttcgaatct accagaacgg aacggcgcac ggtatcggac cagtaatcat
     9841 aatgcgcggt acggcgcagg ctagcctcaa taccgccgag gatcaccggc acatctttcc
     9901 acgcctcttt acaacgctgg gtataaacca gcgtggcgcg atccggtcgc ttacccgcga
     9961 cattatctgg cgtgtaggca tcatcatgac gtaaacggcg atcggcggta tagcggttga
    10021 tcatcgaatc catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca
    10081 taaagtcgtc tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg
    10141 cttccagcat acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc
    10201 cagtaaccaa aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca
    10261 tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt
    10321 cgcgatccgg ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat
    10381 aatcaaaggc cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc
    10441 gccctcctcg ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga
    10501 gccacggtcc gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga
    10561 aggctgaccg aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca
    10621 gcctctgtct tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc
    10681 ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg
    10741 ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc
    10801 acccagactg atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa
    10861 gcgcatcgga agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac
    10921 caaggtagaa accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct
    10981 cgccgcttca ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc
    11041 gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc
    11101 ctgatcgcca gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg
    11161 agagttcgac gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg
    11221 gctttgtaca ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt
    11281 gtattctggc gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc
    11341 cacaccataa tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat
    11401 tccggcaagg ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca
    11461 cagagtagct gcgttctggc gaataggcag tacgggtgcc cagtcagcgt ttggcgacat
    11521 catccgtgcc ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga
    11581 gacattttct gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg
    11641 gatagtcggc cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt
    11701 aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag
    11761 tagcggcgga acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc
    11821 gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt
    11881 tgctccatgt aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac
    11941 agattgccga cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg
    12001 gcgatttttt gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg
    12061 ccatactgac tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt
    12121 cgattaagat taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc
    12181 gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg
    12241 cgggttcctt ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact
    12301 tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt
    12361 aaccagtaca actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc
    12421 gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg
    12481 atatagatag cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg
    12541 ccgaacagtg gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta
    12601 cgcgggctga aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg
    12661 gtaataataa gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat
    12721 tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat
    12781 tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg
    12841 ttgaatacgc tgccggatga cgacttaacg tttcatccgg cgttccttgc tagcgggagg
    12901 aaacagcgcc ctccccggca tattactctt cgctatcacc gctgctggca cggcgaggag
    12961 agtcgatctc aacacgatcg atacgctgca aaccgcgcat caacgtaccg cggcgtccac
    13021 gttcgccagt gactttctgt agctcttccg gacgcagttt aattttgcgt ttcccaacat
    13081 gaatggtcag cgtgctttgc ggcggcagaa cgtacagttg cgccagacca tcctcgccac
    13141 gcgcggcttc tgccgatgga atgttgataa tcttgttgcc tttgcccttc gacagctgcg
    13201 gcagatcgct taccgggaac atcaacatac ggcctgcctg agtgattgcc agcagcatat
    13261 cggaagcatc ttcaatcacc accggcggca taacatgggc attttccggt aaggtgatca
    13321 aagccttacc cgcacggtta cgcgctacca gatcgttaaa ggtgcagacg aaaccgtaac
    13381 ccgcatcgga agccatcagc agtttctgat cgtcgctttc catcagcata tggtcaacgg
    13441 tcgccccagg cggcaacgtt aatttgccgg tgagtggttc gccctgacca cgcgccgacg
    13501 gcagcgtaat cgggtcgatg gcatagctac gaccggtgga atcaacaaac actaccggtt
    13561 ggttactctt acctttcacc gccgctttga agctatcacc cgctttataa ttcaggcccg
    13621 gcgcgtcgat atcatggcct ttagcgctgc gtacccagcc catctgcgac agcacaatgg
    13681 tgacaggttc agacggcagc atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct
    13741 gcaacggcga acgacgatcg tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca
    13801 gcaggttatt cattttacgc tcggaagcca aaatgccctg caactggtcg cgctcttttt
    13861 ccagctcact ctgctcaccg cgaatcttca tctcttccag tttggcaaga tgacgcagtt
    13921 tcagttcgag gatcgcttcc gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg
    13981 gtttcggttc atcttcgtta cgaatgatct caatcacttc gtcgatgttg agaaacgcca
    14041 ccagcaaacc ttccaggata tgcaggcgct tgaggacttt ctccagacga tagttcagtc
    14101 ggcggcgcac ggtatcgcga cggaacacca gccattcgga gaggatttcc agcaggtttt
    14161 tcaccgccgg acgaccatcc agaccgatca tattgaggtt aatgcgatag ctcttttcca
    14221 gatcggtggt agcgaagagg tggttcatca cctgatccat atccacgcgg ttggaacgcg
    14281 gcacaatcac cagacgggtc gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca
    14341 tcggcagctt tttgttgcgc atttgcgcag caatttgctc cagtacgcgc gcacctgaaa
    14401 cctgatgcgg taatgcgctg ataaccaccg cgccatcttc tttcttccac accgcgcgca
    14461 tacgcactga accacgtccg ttctcgtaga ttttacgaat ctcggcgcgc gaagtgataa
    14521 tttccgcttc agtcggataa tccggcccct gcacgatatc cagcagctga tcgagcgtag
    14581 ttttcggctg gtcgattaat gcgattgccg cctgagctac ttcacgcagg ttatgcggtg
    14641 gaatatcggt cgccatgccg acggcaatac cggtggtgcc gttaagcaaa atgtttggca
    14701 gacgggcagg tagcattttc ggctcctgca aagtgccgtc gaagtttggc acccagtcag
    14761 ccgtcccctg ccccagctcg ctcaatagca gctcggaata tttcgacaac cgggattcgg
    14821 tgtaacgcat tgccgcgaac gatttcggat cgtccggcgc gccccagttc ccctgaccat
    14881 caaccagcgg ataacggtaa gagaacggct gcgccatcag gaccatcgct tcataacagg
    14941 cgctatcgcc gtgcggatgg tatttaccca gtacgtcacc gacggtacgg gccgattttt
    15001 taaatttggc gctggcattc aggcccagtt cagacatcgc atacacaatg cggcgctgaa
    15061 caggtttcag accatcacca ataaacggca acgcacggtc catgatcacg tacatggagt
    15121 agtttaagta ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc atatcgctca
    15181 ttaattctga ttcctcaact tattcgccag ccttcaaagg ggcaatattg ccggagatac
    15241 tacattatct gccgcgtcga gtcacaggag atcagcgtga atagcgccgc cggacgtgct
    15301 ccgacggcga taaaggcatc aatgtgcctt gatatacaac tctttgctgt agacttgatc
    15361 ggcatagagg gcagcacaag gttgcgccca tgccacacca ctcggcatgg gggtccgctc
    15421 cgggcgattc gagaagttga ggttatgcga gtccagggaa gcctaaagca tcaaagtagt
    15481 cgactgtaag tatacggttc agcccagttt agctgtgtct ccaccagtgc cccgcgcctc
    15541 ctgccataag tgcggcttgc tcatggatat ccccgagacg gcttacggcg caccggataa
    15601 taaatttcgc gtatacatgg tattccttac cggagaaatt ataagcccaa caacaatacg
    15661 cctgtgacat tgttcgctca aacttatcgc gagtgatttg gctcacactc cggtaaagat
    15721 tccccatcac gccaacggca cataaacatc cgtttccagt cgctgctctg tcaccccttc
    15781 ggcaagattg gtgtagtgaa acagaatcgg tgctttacgc attttctcgc cgcttgcagg
    15841 cagccagtgg cgaataatgc cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg
    15901 gcgggccacg gcataacgtc caccggtaag ttcaccattg ctcacaccat aacgattatc
    15961 gggaatcggt tcgctaacgc tgccgcagat atcaaagcga aacgcttccg gtgcggtggt
    16021 ggcaggggcg tcccaggcga cgccaaaagt ctggctttgg ttaacaggag aaagccccgt
    16081 ctctttacgc cacatgataa atttcgccac actgtagttg agcaactcag ggctacagcg
    16141 gtgcggcaac atcgccacag ggattgatgg gaaatcgata atgtttacat ccagtgtcag
    16201 gtttgtcatt ttgcctccct gcgaaattat tcccgggaac gcgtcccggg agcggtaaca
    16261 attacggatt tactttgcgg atctgtttga cgtcaatttc aacagaattc cagtctttat
    16321 cgacttcacc ctgaatctca accgtatctt tcggcgtcac cgtcacaccg ttccagcgtt
    16381 tgtgatcgat atcaacattg atagtaccgc tggcatcttt aaacacgtag agatcgtcag
    16441 agatgcgttc aacgatattg ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg
    16501 cgctttctac agtcgttacg ctgccgttcg gcccctgaaa tcctccggcc tgactttgcg
    16561 ttaccgatgg gccagaaaaa ccgccctgct ctgctgccat caccggtgca ctgcacaggg
    16621 ccattactgc gattactgct gcgaattttt tcatgtttat tactcccttt aatgtctgtt
    16681 tccgagcatt taacaagata gtccttaaca acttcttaag ggaaaaaaat aaaatttaat
    16741 gctgtacaga gcgcgttaca acacggttta ctggcagcaa atacggttat cgcagggatg
    16801 aaaaaatgcg aattttactg atagaagatg acatgctgat tggtgacggc atcaaaactg
    16861 gccttagtaa aatgggtttt agtgtcgact ggtttacaca aggtcgtcag ggaaaagagg
    16921 cgctttatag cgcaccttat gatgcggtga tcctggattt aaccttacca ggcatggatg
    16981 gtcgcgatat tttgcgcgaa tggcgagaga aaggtcagcg tgagccggta ctgaacctga
    17041 ccgcgcgtga tgcgttagcg gaacgtgtag aagggctgcg tctgggagct gacgattatc
    17101 tgtgtaaacc ttttgcgttg atagaagtcg ccgccaggct ggaggctctg atgcgccgaa
    17161 ccaacggcca ggccagcaac gagctgcgcc acggtaacgt catgctcgac cccggcaaac
    17221 gtatcgccac gctggctggc gaacccttaa cactgaaacc aaaagaattt gccctgctgg
    17281 aattactgat gcgtaacgct ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt
    17341 atacctggga cgaagaggtc accagtaatg ccgttgaagt gcatgtgcat catctgcgac
    17401 gcaaactcgg tagtgatttt attcgtaccg tgcatggtat tggctacaca ttaggtgaga
    17461 aatgaaattt acccaacgtc ttagtctgcg cgtcaggctg acgctaatct ttttaattct
    17521 ggcctcggtg acctggctgc tttccagctt tgtcgcctgg aaacaaacaa cggataacgt
    17581 cgatgaattg ttcgacaccc aactgatgct gtttgccaaa cggttaagta cgctcgatct
    17641 caacgaaatc aacgcggcgg atcgcatggc acagacgcca aataaattaa aacacggtca
    17701 tgttgatgac gatgcgctga cctttgccat ctttacccac gacggcagaa tggtccttaa
    17761 tgatggcgat aacggagaag atattcccta tagctatcaa cgggaaggtt ttgctgacgg
    17821 gcaactggtc ggtgaagacg atccttggcg ttttgtctgg atgacctcac ctgatggcaa
    17881 atatcgcatc gttgttggcc aggaatggga ataccgtgaa gacatggcgc tggcgattgt
    17941 tgccgggcaa ttgatcccgt ggctggtcgc actgccgatt atgttaatca tcatgatggt
    18001 actactgggt cgtgaactcg cgccgctgaa caaactggcg ctggcactac gtatgcgtga
    18061 ccctgactcg gaaaaaccac taaacgcgac tggcgtaccc agcgaagtgc ggccactggt
    18121 tgagtcgcta aatcaactgt tcgcccgcac acatgcgatg atggttcgag aacgacgctt
    18181 tacctccgat gcagctcacg aactacgtag cccgttaaca gcgctgaaag tgcaaaccga
    18241 agttgcgcag ctctctgacg atgatccgca ggcgcggaaa aaagcactgc tccaattaca
    18301 ttccgggatc gatcgcgcta ctcgtctggt tgatcaactg ctcacgctat cgcggctgga
    18361 ctcactggat aaccttcagg acgtcgcgga gatcccgctt gaagatctcc tacaatcgtc
    18421 ggtgatggat atttaccaca cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa
    18481 tgcccacggc atcaaacgca ccggacaacc gctattgcta agtttgttgg tgcgaaattt
    18541 gctggataac ggccccggtg tgacaccaga ggcactggcg cgaattggcg aacgcttcta
    18601 tcgcccaccc ggacaaaccg ctaccggtag tggacttggg ctatcgattg tccagcgaat
    18661 cgccaagctg catgacatga atgttgagtt tgggaatgcg gaacaaggtg gatttgaggc
    18721 gaaggtaagc tggtaacttc tctgtctatg agagccgtta aaacgactct catagatttt
    18781 actaatagca aaatataaac cgtccccaaa aaagccacca accacaaacc agacaaataa
    18841 cgagcgagaa tacatatagc caaaactcga acatttcccg cataaagagt tttcttaaga
    18901 taagaataat aagtggcgta agaagaaaaa atgctgcaat cattgcttgc aatatacgat
    18961 cttcgcctga aacggaaggt atactgcaaa tattgcctcc cgactcaagc atccagtcat
    19021 acttattgaa agcaaaaaaa gaatctatta accaataagt aagcattata tatggcaatg
    19081 cttcaaatat tgttttaatt ttctgcttta acatatatca gtacgctcat aaaaatccat
    19141 ttataattat caacatgaca cgttgatatt ttcatctttt acttattcaa agccatattt
    19201 tcaatcccca aaaaaaccga accaaacgtg taaattattc cggtgcgagt aagaattatg
    19261 aggaatggct atcagtattg tcattttcag aaaatattta tcctgcatcg gtgagtcaga
    19321 gctagatcag acttttgcta aattcgcaaa agactttgca cattttgcta atttcacagt
    19381 accgctctgt gacgtactat agtcgacaaa cgtctcacct tgagattaaa aaatgagcaa
    19441 catcctgatt atcaacggcg cgaaaaaatt cgcccactcc aatggtcaac tgaacgacac
    19501 cctgaccgaa gtcgcggatg gcacactgcg cgacctcggg catgatgtcc gcatcgttcg
    19561 cgccgacagc gactacaatg tcaaagcgga agtacaaaac tttctctggg ctgatgtggt
    19621 gatctggcag atgccaggct ggtggatggg cgcgccgtgg acagtgaaaa aatatattga
    19681 tgatgtattc accgaaggtc acgggactct gtatgccagc gatggtcgta cccgcaaaga
    19741 tccgtcgaaa aaatacggtt ccggcagcct ggtacaggac aaaaaatata tgctttctct
    19801 aacctggaac gcaccaatgg aagccttcac cgaaaaagat cagttcttcc acggcgttgg
    19861 cgttgacggt gtgtatctgc cgttccataa agcaaaccaa ttcctcggta tggaaccgct
    19921 gccgacattt atcgctaatg acgtgataaa aatgcctgat gttccccgct atactgaaga
    19981 atatcgcaag catcttgtgg aaatttttgg ttaactagag ctcaggcttt agaaggagtt
    20041 aaccatgctt accgtaatcg cagaaatccg tactcgtcct ggtcaacatc accgtcaggc
    20101 ggtattggat cagtttgcta aaatcgttcc aaccgtactg aaagaagaag gttgccacgg
    20161 ctatgcgcca atggtggatt gcgctgctgg cgtgagtttc cagtctatgg caccggattc
    20221 tatcgtgatg attgagcagt gggaaagcat cgcgcacctt gaagcgcatc tgcaaacccc
    20281 gcacatgaag gcgtacagcg aagccgtaaa aggcgacgtg ctggagatga atatccgtat
    20341 tctgcagcca gggatttaat cctgccttgt ttgcccggcc atcctgaccg ggcaatgttc
    20401 tttcctttaa acctcaatct ccgccatgtc gcctttctct tgcaaccagt tgcggcgatc
    20461 ttccgagcgt ttcttcgcca gcagcatatc catcatcgcg tcagtacgct gatcgtcttc
    20521 atcatcgata gtcaactgca ccagacggcg agtgttcgga tcaagcgtgg tttcacgcaa
    20581 ttgcatcggg ttcatttccc ccagaccttt aaaacgctgg acgttcggct tgcctttctt
    20641 gcgttttaat tgctcaagta cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc
    20701 tttcccgaga tcaatacggt agagcggtgg cagtgcgacg taaacgtgac cgtgtttcac
    20761 caacgcgcgg aagtgtttta cgaacaaagc gcagagcagc gtggcaatgt gcagaccatc
    20821 agagtccgca tccgcgagga tacagatttt gccataacga agctggctca gatcgtcgct
    20881 gtcaggatcg ataccgatcg ctaccgaaat atcatgcact tcctgcgagg ccagcacttc
    20941 gtcggaagaa acttcccagg tgttaaggat cttacctttc agcggcatga tcgcctgata
    21001 ttcacgatcg cgcgcctgct tggcagatcc gcctgcggag tcaccttcca cgaggaacag
    21061 ttcggtacgg ttaaggccct gcgcggtaca atcagccagt ttgccaggca gcgccgggcc
    21121 gctggtcagc tttttacgca ccactttttt agccgcacgc atacgacgct gggcgctgga
    21181 aatcgccatc tctgccagca actccgccgc ctgaacgttc tggttcagcc acaggatgaa
    21241 ggcatctttc accacgcccg aaacgaatgc cgcacactga cgcgaagaga gacgctcttt
    21301 ggtctgcccg gcaaactgcg gatcctgcat tttaaccgac agaacatagg cgcagcgatc
    21361 ccagatatct tccgccgaca gcttcacgcc gcgcggcaga atgttgcggt attcacagaa
    21421 ctcacgcatc gcgtccaaca ggccctgacg cagaccatta acatgggtac cgccctgcat
    21481 cgttgggata aggttgacgt agctttcggt cagcagttca ccgccttccg gcagccacag
    21541 tagcgcccag tccacagctt cagtatcgcc agcgaaatta ccgataaacg gtttttctgg
    21601 cagcgtcggt aaaccgttta ccgcttccgc caggtaatca ttcagaccgt cctgatagca
    21661 ccagcgctgt tcggtgttgt tgatctcatc tttaaaggta atctcaacgc cggggcacag
    21721 tactgctttg gccttcagca catgcgtcag gcgtgaaaca gaaaaacgcg ggctgtcaaa
    21781 gaaggtttca tccggccaga agtgcacgct ggtaccggta ttgcgtttac cgcaagtgcc
    21841 gacaacctgt aaatcctgca ccttttcgcc attttcaaag gcgatgttat aaacctgacc
    21901 atcgcggcgc acgttaactt ctacgcgctt cgacaaggcg ttaactaccg aaatccccac
    21961 gccatgcagg ccgccagaga actggtaatt tttgttagag aatttaccgc ctgcatgcag
    22021 acggcaaaga atcagttcaa ccgccggtac tccttcttcc gggtgaatat ccaccggcat
    22081 cccgcgccca tcgtcaatca cttctaacga ctggtcagca tgtaagatta catccacgcg
    22141 ttttgcgtga cccgccagtg cttcatcgac actgttatca ataacttctt gccccaaatg
    22201 gttagggcga gtggtatcgg tatacatccc cggacggcgg cgaaccggct caagcccggt
    22261 gagtacctca atggcatcag cgttataagt ttgcgtcatg gtttaagtta gcaattcgag
    22321 ttgatcgtca gagatggtgc agaccaagaa aatcgacgat cgggttgaaa taatcttcga
    22381 agcccgtgaa tgcgtggttg ccgccttcta tgacagtctg gcggcaggaa gcatagtacg
    22441 ccaccgcctg gcggtaatcc agcacttcat ctcccgtctg ttgcagcagc cagatcaaat
    22501 ccggcgcttc cagcgggtca atctgcatga ctttgagatc gtaaatatgg cgtgactcta
    22561 gcacatattg ctgcccggtg taggggttct cgttctgacc gagatagtcc gtcagcaatt
    22621 caaacgggcg taccgccggg tttaccacca ctgcgggcag cataaaacat tgcgacaacc
    22681 aggtggcgta atatcccccc agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt
    22741 ccaggacaat ggattccagc agttctgccg cgtcggaagg atacggcggc aactgcggaa
    22801 tgatcatctc aacgtcaggg tgatgttccg ccagccagtt ttttaacaag ctcgcttttg
    22861 cagagcgcgg cgagctgttg aaaccgtgta aataaagaag cgtagacatc agtagccttc
    22921 tgaagcggta tcaggttgga aacgtgtgtc cgccaggcga tgcacctcgg tggtcagcgt
    22981 gccatcagca tgtaactcga gagtacgcca gccgggcgcg atagtatcca gcgtaaagtt
    23041 ggaacagtgc ggcttaaact gcacacaggt cgacggcgtt gccagcaggc ggcgaccgtt
    23101 ccagtcgaga tccagctcct gatgaatatg accgcacagc aagtatttga cgtgcggaaa
    23161 cttcgccagc acggtatcca gttcgcccgc gttacgcaga ctgtgttgat cgagccaact
    23221 acaacccgca ggtagcggat gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc
    23281 ggccagttta cgttccagcc actcaagctg aaactcgctc agctcaccgt gcggcacgcc
    23341 aaacacctgg ctatccagca acaggatttg ccattgctca ccaataaaca cccgcttcgc
    23401 cggggagata cccgcatcct gtaacgcgct gtacatcgcg ggctggaaat cgtggttgcc
    23461 cggcagccag acgcagggcg cacgaaaact tgcgatgcct tcagcgaaat gctgataggc
    23521 cgcagaggat tgatcctgcg ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg
    23581 gtgtggacga atcgcctcca gcaccgcctg gtagctttcc caggtgttta cccctaacag
    23641 ggcttcgtgc ttttgtgcaa acaggtgagt atcggtaatt tgtaaaatcc ttcctctggc
    23701 ctcaccagcc agaggaaggg ttaacaggct ttccaaatgg tgtccttagg tttcacgacg
    23761 ctagtaaacc ggaatcgcca tcgctccatg tgctaaacag tatcgcaacc agtccgctaa
    23821 aaactggtta atttgatgct tttcgtcgcg ttgatgcaac tttttattag gataatcata
    23881 ccgcgctttg aagcgaaaaa tctgctggct tgaacacact tcagccacca tcgcgtcatg
    23941 atacagacgc accgtcattg acggaaggct ccagtaactg atcgcgggcg cagtctgttc
    24001 tattgtcacc agggtagtgt atcgggtcga ttccacaatc gtcagccgat attgtgcgtt
    24061 tgccacctga tagcttacag tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa
    24121 ttgtgaaaag ttcatctcgc acaggcgcat catttcagga aagtcaggtg tgtaacgctt
    24181 catttatgcc cactcatttt ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat
    24241 gaccgacgct gcgttgtcga ttttcccctc ttctacccac tggtatgcct gttcccggct
    24301 taccacatga acgcgaatat cttcgttttc atcagccaga ccatgaatac cgcttgcggt
    24361 cgtggcgtcc acttcgccca ccataattga cgaacgctca ctggtgcccc ccgggcttgc
    24421 caggaaactt aacaccggtt tggtccgttt gactatcagt cccgcctctt caatcgcttc
    24481 gcgacgggca acatcttcca cactttcacc ctcttcaatc atcccggcaa ccatctccag
    24541 tagccagggg gtttcgctgg tgtcgtacgc ggcaatccga atctgctcaa tcagcacaac
    24601 ttcatcacgc actgggtcaa agggtagcaa gactgcggcg tgaccgcgct caaaaatttc
    24661 ccgccgtacc tcatgactca tttgcccgtt gaatagacga tgacgaaatc tataaagatc
    24721 taatgaaaaa aagccgcgat aaagtgtttc tcgtgcaata atttctacat cgtttttgcc
    24781 aaatgtaacg ggcaggttgt ctggcttaag cattgttaat gtcctagcac taatagtgaa
    24841 ttaaatgtga atttcagcga cgtttgactg ccgtttgagc agtcatatgt taaattgagg
    24901 cacattaacg ccctatggca cgtaacgcca accttttgcg gtagcggctt ctgctagaat
    24961 ccgcaataat tttacagttt gatcgcgcta aatactgctt caccacaagg aatgcaaatg
    25021 aagaaattgc tccccattct tatcggcctg agcctttctg ggttcagttc gttgagccag
    25081 gccgagaacc tgatgcaagt ttatcagcaa gcacgcctta gtaacccgga attgcgtaag
    25141 tctgccgccg atcgtgatgc tgcctttgaa aaaattaatg aagcgcgcag tccattactg
    25201 ccacagctag gtttaggtgc agattacacc tatagcaacg gctaccgcga cgcgaacggc
    25261 atcaactcga acgcgaccag tgcgtccctg cagttaactc aatccatttt tgatatgtcg
    25321 aaatggcgtg cgttaacgct gcaggaaaaa gcagcaggga ttcaggacgt cacatatcag
    25381 accgatcagc aaaccttgat cctcaacacc gcgaccgctt atttcaacgt gttgaatgct
    25441 attgacgttc tttcctatac acaggcacaa aaagaagcga tctaccgtca attagatcaa
    25501 accacccaac gttttaacgt gggcctggta gcgatcaccg acgtgcagaa cgcccgcgcg
    25561 cagtacgata ccgtgctggc gaacgaagtg accgcacgta ataaccttga taacgcggta
    25621 gagcagctgc gccagatcac cggtaactac tatccggaac tggcggcgct gaatgtcgaa
    25681 aactttaaaa ccgacaaacc acagccggtt aacgcgctgc tgaaagaagc cgaaaaacgc
    25741 aacctgtcgc tgttacaggc acgcttgagc caggacctgg agcgcgagca aattcgccag
    25801 gcgcaggatg gtcacttacc gactctggat ttaacggctt ctaccgggat ttctgacacc
    25861 tcttatagcg gttcgaaaac ccgtggtgcc gctggtaccc agtatgacga tagcaatatg
    25921 ggccagaaca aagttggcct gagcttctcg ctgccgattt atcagggcgg aatggttaac
    25981 tcgcaggtga aacaggcaca gtacaacttt gttggtgcca gtgagcaact ggaaagcgca
    26041 catcgtagcg tcgtgcaaac cgtacgttcc tccttcaaca acattaatgc ttctatcagt
    26101 agtattaacg cctacaaaca agccgtagtt tccgcgcaaa gctcattaga cgcgatggaa
    26161 gcgggctact cggtcggtac gcgtaccatt gttgatgtgt tggatgcgac caccacgctg
    26221 tacaacgcca agcaagagct ggcgaatgcg cgttataact acctgattaa tcagctgaat
    26281 attaagtcag ccctgggtac gttgaacgag caggatttgc tggcactgaa caatgcgctg
    26341 agcaaaccgg tttccactaa tccggaaaac gttgccccgc aaacgccgga acagaatgct
    26401 attgctgatg gttatgcgcc tgatagcccg gcacccgtcg ttcagcaaac atccgcacgc
    26461 actaccacca gtaacggtca taaccctttc cgtaactgat gacgacgacg gggcttcggc
    26521 cccgtctgaa cgtaaggcaa cgtaaagata cgggttatct gccgcattct tcccccttct
    26581 cgcttcaatt tcgaccagcc atcctctatt ctgatgggta tttaccactg gtcccggaag
    26641 acaaaaatga aacggacaaa atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca
    26701 cgccatctga caccagtcgc tctcgcggtt gccactgttt ttatgctggc tggctgtgaa
    26761 aagagtgatg aaacagtgtc tctctatcaa aatgctgacg actgttcagc tgcaaaccca
    26821 ggcaaaagcg ccgaatgtac caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact
    26881 gcgccgaaat acgccacccg tgaagactgt gttgctgaat ttggtgaagg tcagtgtcag
    26941 caagcaccag cccaggctgg catggcacca gaaaaccagg cgcaggctca gcaatccagc
    27001 gggagtttct ggatgccgct gatggccggt tacatgatgg ggcgtctgat gggcggcggc
    27061 gcgggatttg cacagcagcc gctgttctcc tcgaaaaacc cggccagtcc ggcttacggt
    27121 aaatataccg acgcgacggg taaaaactat ggcgcagccc agccaggccg caccatgacc
    27181 gtaccgaaga cggcaatggc accaaaaccg gcgaccacca ctaccgttac ccgtggcggt
    27241 tttggtgaat ctgttgccaa acaaagcact atgcagcgta gcgcaaccgg cacctcttct
    27301 cgttcaatgg gtggctgata ccgatggaaa gagtcagtat taccgagcgc ccggactggc
    27361 gtgagaaagc ccacgaatac ggtttcaatt ttcacaccat gtacggcgag ccgtactggt
    27421 gtgaagatgc ttactacaag ttgaccctcg cccaggttga aaagctggaa gaagtcaccg
    27481 ccgaactgca ccagatgtgc ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga
    27541 tgaccaaatt ccgcatacca aaacacacct ggagttttgt gcgtcaatca tggctgactc
    27601 accagccatc gctttattcg cgtcttgatc tggcgtggga tggcactggc gaacctaaac
    27661 ttctggaaaa taacgccgat acgccaacgt cactatacga ggcggcgttc tttcagtgga
    27721 tctggctgga agatcagctt aacgccggta acttgccgga gggcagcgac cagtttaaca
    27781 gtctgcaaga aaaactgatc gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt
    27841 tgctgcatct cacctgctgt cgcgacacgg tggaagatcg cggaaccatt cagtatttgc
    27901 aggactgcgc aaccgaagct gaaatcgcca ccgagttcct ctacatcgac gagataggtt
    27961 taggcgaaaa aggccagttc acggatttac aggatcaggt gatttctaac ctgttcaagc
    28021 tttatccgtg ggaatttatg ctgcgcgaaa tgttctccac caagctggaa gatactggcg
    28081 tacgctggct ggaaccggcg tggaagagca ttatctccaa caaggcactt ctaccgctac
    28141 tgtgggagat gttcccgaat cacccgaacc tgctgcccgc ttattttgcg gaagatgatc
    28201 atccgcaaat ggaaaaatat gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt
    28261 cgatcattga gaacggcaaa accattgaag cagcggaagg tccgtatggc gaagaaggga
    28321 tgattgttca gcaattccac ccgttaccga aattcggcga cagctatatg ctgattggta
    28381 gctggctggt gaacgatcaa cctgccggaa ttggcattcg tgaagaccgt gcgttgatca
    28441 cccaggatat gtctcggttt tatccacata tttttgttga ataagccacg ataccggatg
    28501 gcactcgcca tccggtaatt gttagcctat ctgcaccgac agcatactta aactgcccat
    28561 ttctatacca tcgaccggaa tggtaattgg ctcctgccca tcccacacac ctaacacata
    28621 caacaacggc agatagtgct ctggcgttgg gttcgataac gcgccacctt catggtcgag
    28681 ataattcacc agaggatgct gctctaccgg cccttgccac gtcagattcg ctttcacata
    28741 ctcattaaac gacgtcgccc acggatacgg tgaactatca ccgtgccact tcactgtgcg
    28801 caggttatgc accacgttac cgctggcgac caacattatt ccttcatcac gcagcgccgc
    28861 gagtttgcgc cccatttcga aatgccaggc ggcaggtttg ctactgtcga tactcaactg
    28921 caccataggg atatcggcgt caggatacat cttaatcagc acgccccacg agccgtggtc
    28981 aaagccccag gcttctttat ccagcgtcac cgggatcggc gctaacagct caaccagacg
    29041 ctgtgccagc gcaggcgaac ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa
    29101 gccaccaaag tcatgaatcg tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt
    29161 aaaccagtga gccgaaacca ccacaatcgc ttgcgggcgt ggcaatgtca tccccaactt
    29221 ctgccagctg cgggtataca aattatcttc cagcacgttc atcggactac cgtgacctaa
    29281 aaacaatgct ggcatacgtg ttgaagacat gatgatatcc ttaactaaag gtgtcatttt
    29341 gatatcctca caatacgctt gttcggcgga gtaagaaccc ggataacaat gatgatgatc
    29401 atcagttatt ttgacgatct gcctgaaggt gaagatttat aaggagttgt cgatgtcagt
    29461 acctctcatt ctgaccatac tggcgggggc agccacgttt attggcgcgt ttctcggcgt
    29521 tctcggacaa aaaccctcga accgcttact ggcgttttcg ctaggttttg cggcggggat
    29581 catgttgctc atctcattaa tggaaatgct tcctgccgca ctagcagctg aaggaatgtc
    29641 gccggtgttg ggttatggaa tgtttatctt cggtctgctt ggctactttg gcctggaccg
    29701 catgttgccg catgcgcatc cgcaggattt aatgcaaaaa tcggtgcagc tgttgccaaa
    29761 atcgatcaag cgcacagcca ttctgctcac tctcggcatc agtctgcata acttcccgga
    29821 agggattgcc acctttgtca cggcgagcag caacctggag ctgggatttg gcatcgcact
    29881 ggccgtcgcg ttgcacaata tccctgaagg tctggcagtg gcaggcccgg tttatgcggc
    29941 aacggggtct aaacgcaccg cgattctgtg ggcggggatt tctggactag cagaaattct
    30001 tggtggtgtg ctggcgtggt taatcctcgg tagcatgatt tccccggtgg tcatggcggc
    30061 aatcatggcg gcggttgcag gaattatggt ggcgctctcg gttgatgaat taatgccgct
    30121 cgccaaagag attgacccta ataataaccc cagctatggc gtactatgtg gaatgtcagt
    30181 gatgggattc agtttagtgc tgctacaaac ggcgggaatt ggttaaaaac aacatcagaa
    30241 gttgtccagc acatggacaa cttctgccag cttcacgaaa gccttattct accttttcgg
    30301 ctgcctcttc tctcgtactg tttaagtatt tgttccgcgc ttcaagcagt gcgtctctgt
    30361 cctggcgaaa cgcccgacaa taacgtttca aatgcaggaa ataacccacc acaaccagag
    30421 caatcaatac aatccagtac caggcgataa acatcctttt acccttaata tttaacaata
    30481 ctaaacattt agcgcataaa tttcacatat cctttttcgg atatattcat atggtcgcat
    30541 aataacggaa caattatatg attttgatca taatgaaaaa gaatgcctga tattaatgaa
    30601 attttctcat agaaagagaa gcggaattaa catcttgcat agcagggcaa aataaaaacc
    30661 gaagccagct acaacttcgg tttcttgatt aaggcagtaa ttaagcagcg gttttcagct
    30721 ggctttacgc tcatgtgcct gacggtatgc caccaggtct tcaatagtca cgagcgccat
    30781 attgtgttta ttggcaaact caatgcactc tggtgcacgc gccatcgtgc catcgtcatt
    30841 agtcagctca cacagcacac cagccggttt aaagcctgcc agcgtcatca aatcaatagt
    30901 cgcttcagta tgaccgccac gcgtcagtac accacctgcc tgcgcgcgaa gtgggaaaac
    30961 gtggcccggg cgattcagat cagaaggttt tgcgccatcg gcaatcgctg cgcgaacggt
    31021 cgtaatacga tcagcggcag aaacaccggt agtcacacct tcagccgctt caatggtcac
    31081 ggtaaaaccg gtgccatagg cgctggtgtt attttctacc atcattggca gatcaagttg
    31141 tttacggcga tcttcagtga tgcacaggca aacaataccg ctaccgtggc gaatggtcag
    31201 cgccatctgc tcaacggtca tggtttctgc cgggaagatc atatcgcctt cgttttcacg
    31261 gtcttcatca tcaagcacca ttacaccgcg tccttcacgc agcgcagcca gtgcattttc
    31321 aacacgttcg aaaggcgtac caaaagagga aagtagcgtc tgattcatgg taaaaaaacc
    31381 tcactaaaat tatggttacc agaatcaggg cagtcttagg agtggcggca tatagccaaa
    31441 ataacgtgag cgggtccatg cccgacagaa tcgttactct ctcccatccg gactctaacc
    31501 gtcggccccg gaattacacc ggatctgctg tcctttgagt tcgcacccaa agcgctcgcg
    31561 ggctttcaac tgagttgatt taccgccggt ggggaatttc gccccgccct gagaataagc
    31621 ggattcacta taacgctaat gattagcggc agcaacgcat agcttcacat aattctggtt
    31681 tatgacttac ccttatcgca ctacaatggt actcaacacc tatcag
//
LOCUS       DAJTYM010000040        31179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTYM010000040 DAJTYM010000000
VERSION     DAJTYM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            383..2041
                     /locus_tag="OO575_001891"
     CDS             383..2041
                     /locus_tag="OO575_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6664882.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2038..2994)
                     /locus_tag="OO575_001892"
     CDS             complement(2038..2994)
                     /locus_tag="OO575_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6664883.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3145..4260
                     /gene="macA"
                     /locus_tag="OO575_001893"
     CDS             3145..4260
                     /gene="macA"
                     /locus_tag="OO575_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6664884.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4257..6203
                     /gene="macB"
                     /locus_tag="OO575_001894"
     CDS             4257..6203
                     /gene="macB"
                     /locus_tag="OO575_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6664885.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6276..6500)
                     /gene="cspD"
                     /locus_tag="OO575_001895"
     CDS             complement(6276..6500)
                     /gene="cspD"
                     /locus_tag="OO575_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6664886.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6823..7143
                     /gene="clpS"
                     /locus_tag="OO575_001896"
     CDS             6823..7143
                     /gene="clpS"
                     /locus_tag="OO575_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6664887.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7174..9450
                     /gene="clpA"
                     /locus_tag="OO575_001897"
     CDS             7174..9450
                     /gene="clpA"
                     /locus_tag="OO575_001897"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6664888.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9853..9940)
                     /locus_tag="OO575_001898"
     tRNA            complement(9853..9940)
                     /locus_tag="OO575_001898"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9904..9906),aa:Ser,seq:gga)
     gene            complement(10194..10412)
                     /gene="infA"
                     /locus_tag="OO575_001899"
     CDS             complement(10194..10412)
                     /gene="infA"
                     /locus_tag="OO575_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6664889.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10697..11401)
                     /gene="aat"
                     /locus_tag="OO575_001900"
     CDS             complement(10697..11401)
                     /gene="aat"
                     /locus_tag="OO575_001900"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6664890.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11443..13164)
                     /gene="cydC"
                     /locus_tag="OO575_001901"
     CDS             complement(11443..13164)
                     /gene="cydC"
                     /locus_tag="OO575_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6664891.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13165..14931)
                     /gene="cydD"
                     /locus_tag="OO575_001902"
     CDS             complement(13165..14931)
                     /gene="cydD"
                     /locus_tag="OO575_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6664892.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15054..16019)
                     /gene="trxB"
                     /locus_tag="OO575_001903"
     CDS             complement(15054..16019)
                     /gene="trxB"
                     /locus_tag="OO575_001903"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6664893.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16563..17057
                     /gene="lrp"
                     /locus_tag="OO575_001904"
     CDS             16563..17057
                     /gene="lrp"
                     /locus_tag="OO575_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6664894.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17192..21220
                     /gene="ftsK"
                     /locus_tag="OO575_001905"
     CDS             17192..21220
                     /gene="ftsK"
                     /locus_tag="OO575_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6664895.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDAPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21375..21986
                     /gene="lolA"
                     /locus_tag="OO575_001906"
     CDS             21375..21986
                     /gene="lolA"
                     /locus_tag="OO575_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6664896.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21997..23340
                     /gene="rarA"
                     /locus_tag="OO575_001907"
                     /gene_synonym="mgsA"
     CDS             21997..23340
                     /gene="rarA"
                     /locus_tag="OO575_001907"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6664897.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23431..24723
                     /gene="serS"
                     /locus_tag="OO575_001908"
     CDS             23431..24723
                     /gene="serS"
                     /locus_tag="OO575_001908"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6664898.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24963..27406
                     /gene="dmsA"
                     /locus_tag="OO575_001909"
                     /pseudo
     CDS             24963..27406
                     /gene="dmsA"
                     /locus_tag="OO575_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27417..28034
                     /gene="dmsB"
                     /locus_tag="OO575_001910"
     CDS             27417..28034
                     /gene="dmsB"
                     /locus_tag="OO575_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6664899.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLLRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28036..28827
                     /locus_tag="OO575_001911"
     CDS             28036..28827
                     /locus_tag="OO575_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6664900.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28863..29489)
                     /locus_tag="OO575_001912"
     CDS             complement(28863..29489)
                     /locus_tag="OO575_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6664901.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29804..30952
                     /locus_tag="OO575_001913"
     CDS             29804..30952
                     /locus_tag="OO575_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6664902.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7689 a         7644 c         8370 g         7476 t
ORIGIN      
        1 ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt ttaaaaacga
       61 aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca acagcgggtt
      121 gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt taatccttta
      181 ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt tgcgggctta
      241 tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg ccatgcttaa
      301 atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg taaaggataa
      361 gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg gttttcgcgg
      421 tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg agaacgcgtg
      481 gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat cagatctcta
      541 ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc gagaacatca
      601 tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg ttcgccgtta
      661 tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta ttttttatcg
      721 tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt ttctcgataa
      781 agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc tggtgcgttt
      841 aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta acggcacggt
      901 gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg cccgtgagtt
      961 gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt ccgcaatggt
     1021 gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat atcgtttaat
     1081 gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca tcaaccggat
     1141 gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt ttgctacttt
     1201 attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat tgctaattga
     1261 agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc tgttgaatct
     1321 tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt taactccagt
     1381 ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc gtctggggcc
     1441 aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt ttaatcgtcc
     1501 gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa cctgggttat
     1561 caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca aggtcattga
     1621 gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg ggattgaatg
     1681 gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg tacgcagcct
     1741 gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg cgctggatat
     1801 ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg cgcaaatccc
     1861 ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc gctcttccaa
     1921 acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg actccgtacc
     1981 aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc gcgcggatta
     2041 accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat agcgccgacg
     2101 gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta tctgtgctgg
     2161 aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga acgcgttgta
     2221 atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa tatgcgtttc
     2281 attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac aggccgcttc
     2341 catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct ggatttcctg
     2401 atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc ttttcccctg
     2461 atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt tgcggaacag
     2521 gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa gcgtgaactg
     2581 ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca aatataagct
     2641 gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta acgcctccag
     2701 caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca caggcagtcg
     2761 cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt tcatccactc
     2821 aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa aactacagcg
     2881 atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa gtgacaggct
     2941 gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat tcatgaaatc
     3001 aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat caatagtgtg
     3061 attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg acccggattg
     3121 gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa gcgttacgtt
     3181 attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct taacgcaccc
     3241 gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag cgtgctggcg
     3301 accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag cggtcagttg
     3361 aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt aggggtcatt
     3421 gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat ggagctacgc
     3481 gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta ttcccgtcag
     3541 caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc cgcgacggag
     3601 atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg caatcaggcc
     3661 tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc gatggctggg
     3721 gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca gcaagcaccg
     3781 aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt ttctgaagcg
     3841 gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg cgatccactg
     3901 acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt aaacgacgct
     3961 attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg gctggatatg
     4021 accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat ccctctgtcg
     4081 gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg taatggtgaa
     4141 acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga gattgtcaaa
     4201 gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc tgcacaatga
     4261 cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat gagcaggttg
     4321 aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg attgttggcg
     4381 cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat aaggccacca
     4441 gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat gcgctggcgc
     4501 aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt ccgcatttaa
     4561 ccgtcgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg aaacagcgac
     4621 tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca gagtattatc
     4681 cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca ttgatgaacg
     4741 gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat tctggcgaag
     4801 aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt atcgtcaccc
     4861 acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac ggcgaaattg
     4921 tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag cctgttgtca
     4981 atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg acgatggcat
     5041 ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg attattatcg
     5101 gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa atggtgctgg
     5161 cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa gattttggcg
     5221 atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc atccaaaaac
     5281 aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg cgttataaca
     5341 atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat gtctatggca
     5401 tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt gcgcaggtcg
     5461 tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat gtggttggcg
     5521 aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa gaaaaacagt
     5581 cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg atgtccgggc
     5641 gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa ggatttgaca
     5701 gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt aagaaggatt
     5761 tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca cgtactttac
     5821 aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt ggtgtaatga
     5881 atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc atggctgtag
     5941 gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg gtttgcctgg
     6001 tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg cagcttttct
     6061 tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt ctctgctcga
     6121 cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga ctggatccag
     6181 tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgatc gggctggcat
     6241 tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc ttctacttcg
     6301 acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc aaactgaacg
     6361 gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata atgagcgaaa
     6421 atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt gttgaaccac
     6481 ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga
     6541 ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa atacaattta
     6601 gagaattagg gcgagccgtc aagcatttga caggggccag ggggcaggta tgaatcaaaa
     6661 atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag
     6721 atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca
     6781 gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac
     6841 tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct
     6901 atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg
     6961 ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac
     7021 taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg
     7081 atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc
     7141 tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt
     7201 ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac
     7261 ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg
     7321 gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg
     7381 cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa
     7441 cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg
     7501 gtcgctatct tcagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc
     7561 agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga gccgacacag
     7621 tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg
     7681 gagaatttca cgacgaacct gaaccagctt gcgcgtgtgg gcggaatcga cccactaatt
     7741 ggtcgtgaga aagagctgga gcgtgctatt caggttctct gccgtcgccg taaaaacaac
     7801 ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg tcttgcctgg
     7861 cgaattgttc agggtgatgt gccggaagtg atggctgact gtacgattta ctctctcgat
     7921 atcggttctc tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg
     7981 ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc
     8041 attatcggtg cgggtgcagc gtctggtggt caggtcgatg cggctaacct aatcaaaccg
     8101 ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga gttcagcaac
     8161 attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg
     8221 tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac
     8281 gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatt
     8341 aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcagg cgctcgcgca
     8401 cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg
     8461 gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg cgataccctg
     8521 aaaaaactca gcgatcgcct gaaaatgctg gtcttcggcc aggataaagc cattgaggcg
     8581 ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt
     8641 tcgttcctgt ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg
     8701 aaagcgttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc
     8761 gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg
     8821 accgatgcgg taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg
     8881 catccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac
     8941 aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccactaa cgccggggta
     9001 cgggaaactg agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg
     9061 gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg
     9121 ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat cgtcgagttg
     9181 caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg
     9241 ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg tgtcatccag
     9301 gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt
     9361 caggtgacgg ttgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca
     9421 caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg gtgaagtccg
     9481 taatctcgtc aggggttacg gactttttat ttatggggtg gaacggtgaa aaccctattt
     9541 ttggaggtga aggtaagttg ttgataatta gtgctgctgg aaggtaagga taaaaaaggg
     9601 tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt gtagtatttg
     9661 agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc tttgcgaacg
     9721 cgttcactgc cgtacaggca gcttagaaat tcacaggtaa catactccac ccgcccacca
     9781 tgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt cgtacgagct
     9841 cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc cgtatacaca
     9901 ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt gctaccaaac
     9961 ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc aagaagaatt
    10021 ttaatgataa ttattgtttg ctcatactgt aaacaacttg tgcagtatat ctacatcgag
    10081 acaagttatg gacttatact tccaaagtac ttcatacata tcacaaaata aaaaggccgg
    10141 ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa caatcagcga
    10201 ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac agtcactttg
    10261 tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg tgcagtaacc
    10321 acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc aagaacggta
    10381 ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc actaccgtaa
    10441 tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc gcgctaaatc
    10501 gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca ccaaaagcgc
    10561 aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa aattccttat
    10621 cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac tctggggaga
    10681 aatgtgccga aaacattcat tcttgtggtg aaaacaagca ccgtggtacc cagaaattat
    10741 tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg cggggaattt
    10801 cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata agcttaccgc
    10861 catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca ttttccatcc
    10921 ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac ataccgccga
    10981 caagctcatc ttcacgccag acttcaatgg agtgggcatg accgagttcg tgaaggcgat
    11041 ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg ctggcacagc
    11101 cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag cgtttatgaa
    11161 atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg cggggatcgg
    11221 gcgaccacca gaggatgggg tcgcctggag aaaaccacgg aaaaatacca cgctggtaag
    11281 ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg ttaggctcac
    11341 gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga accaagcgca
    11401 tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac cctgcttgaa
    11461 ctggtaataa cgcccctgtc tggcaagcag ttctgcgtga gtaccttgct caataatttg
    11521 cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc gatgggtgac
    11581 cattaacacc gttttctcac gcatcatttt tgcaagcaat tcaaggatct ggctttcggt
    11641 tgtggcatct aagccttcgg taggttcatc cagcaacacc agtggcgcat catgtaacag
    11701 cgtacgggcg atagccagac ggcgcagttc accaccggag agctggcgtc cgccttcacc
    11761 taaccaactg ttgagacctg catcctcgag cagcttctcc agaccaacgc gacgcaagat
    11821 ctccgacaga gcctcatcac tactgccagg cgaggcgagt aacagattat cacgcagtgt
    11881 ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta gagccgcttc
    11941 attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg ggtcccatgc
    12001 gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga gaatcgctat
    12061 atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg attgctccgg
    12121 ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga tcggcaacac gagtttgggt
    12181 atcagggaag gtgacctccg gtttttgatc cgttaagtca gagatacgta cggcagaggc
    12241 aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt caaacgcggc
    12301 taacgcacaa aagacaaaca gggcaattaa tgcgccgggt tgagcattgc cgccaacgcc
    12361 gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagca gcattatcgc
    12421 ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt gaatttctgt
    12481 attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct cagcttgccc
    12541 ttgcagccag gccgtcagtt gttggcgata ctgtccgcga agatgagtca gattttgccc
    12601 ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca gtaacataac
    12661 gctgcccagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg tcaccaccat
    12721 aatcaccaca aaagcgccca ccagcggcga gataacgcgc aggtaaagat gatcgagcgt
    12781 atcaacatcc gccaccacgc ggttgagcaa ttcgccctga cgatagcgcg ccagtccggc
    12841 aggggagagg ggcaacaatt tgctgaaggt gtaaatgcgc agatgctgca acacgcggaa
    12901 agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg tgattgcggc
    12961 accacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg caacccccgc
    13021 aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac tggcgagcag
    13081 cgtcacaatt gccagcacaa taccaagact taacatccat ttatgacgtt tatacagtgc
    13141 cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca gtaatgtggc
    13201 gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa tccggccatc
    13261 ctgcataacc caaatgacat cccagtcagc aagatcttct aactggtggg tgaccattaa
    13321 cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt cactgtgagc
    13381 atcaaggctg gcagcgggtt catccaacag taatagcgaa cagggattta gtaacgcacg
    13441 ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt cgccaacagg
    13501 tgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt tatccagcgc
    13561 tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt agtacgttat cccgcaattt
    13621 tgctgccggt aattgtgggt tttgcccaac ccaggagaga tgtttacgcc atgattctgg
    13681 tgataaatca cgtaattcta tcccgttgat tcgtaatgat ccctgatatg agagaaaacc
    13741 agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg cggccaacca acactgcacg
    13801 ttggcctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt ccggcgacgt
    13861 gataaacaga tcctcggcct caatggtcag cggatcggtc aatgctaatt ccgcctcacc
    13921 gcgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag ctgcaccaac
    13981 agcctgggct ttagcatgat aaaacgtacc gagatcgcgt aatggctgga aaaactctgg
    14041 cgcaaggatc agggccagaa aacctgcagc cagcgtcacc ccagtatcgt agtgaccaaa
    14101 atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa ttgacagcga
    14161 ggtaaaaaat tcgagaatgc cggaggataa aaacgccagt cgtagcactt ccattgtccg
    14221 ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac cacgaccaaa
    14281 aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta agcgagcaag
    14341 agcgagaaag ttacgccggt tagcatcggc agcccccatt ccaaccagcg ccataaacaa
    14401 cggaattagc ggtgctgtgc ccagcagaat gagcgccgca gcccagttag aggggaagat
    14461 cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca gatagcgtgc
    14521 atagtaatca tgcatatcgt caatttgctc gagtaccagc gtcgcccagc tccccgcagg
    14581 tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct gacggcggat
    14641 ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc agaccaccca
    14701 tgcgcgcaat acaaaggtca gaaccagtaa cgtaaaggga agcagcaggg cttcacgggg
    14761 aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct gggcaatgat
    14821 caatatgccg cttacaaagc ccagcagacg agaaatattc agccaacgtt gggagatgac
    14881 gctttgctgt tttaaccagc gggttaactc tttttgacga gatttattca ttgcacgctt
    14941 agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacggggaaa aaataaaggc
    15001 gacccatagt cgcaaggtgt cgccttcttt acttttgtta ctgatttgta aaattatttt
    15061 gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca gccagtaccg
    15121 gccgaagtaa tggcctggcg ataaatgtga tccatcacgt cgcctgcggc aaagacgcca
    15181 ggaatgctgg tctgggtggc attaccatga atacccgact gtactttgat gtagccgttt
    15241 tccagttcca gctgcccttc gaaaatcgca gtattcgggc tgtgaccgat agcaacaaac
    15301 agaccggcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg cagacgaacg
    15361 ccagtgacac ccatttgatc gccggtcact tcttccagcg tacggttggt gtgcagaatg
    15421 atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc cgcgcggaaa
    15481 ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata cagcgcctct
    15541 tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata gaagaaaccg
    15601 tcgcaggttg cacaagcaga aaccccacgg cctttaaagg cttcttcaga gggcaggccg
    15661 agatagcgtg cagaagctcc ggtggcaata atcagcgcgt cgcaagtgta ttcgccgtta
    15721 tcgccattca gacggaacgg acggttttgc agatccacct tgttgatatg atcaaaaatg
    15781 atctcagttt caaacttggt ggcatgttcg tgcatgcgcg ccattaataa cggaccggtc
    15841 agatcgtttg gatcaccagg ccagttttcc acttccgtgg tggtggtcag ttggccgcct
    15901 ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgccgcgta gacagcagcg
    15961 gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt cgtgcccatg
    16021 agatccccat agttgttggc agacaatggg caggattgta gggaatttac agacgtaaaa
    16081 aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata agcaggtcaa
    16141 attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc cgattaaatg
    16201 atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc gatgttttgc
    16261 tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaca gtattcttat
    16321 atgcgcataa ccatgtatgt aaataccatg tttaccgtgc tagtgaaatc tacgtatggc
    16381 gtggacagac gccattcgtg atgtcgatag ctgccacaag gcaacggtct tctcaccgta
    16441 gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac gggtagcgac
    16501 tctgaacagt gatgtttcag ggtcagacag gagtagggaa ggaatacaga gagacaataa
    16561 taatggtaga tagcaagaag cgccctggca aagatctcga ccgtatcgat cgtaacattc
    16621 ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa cgtgtgggac
    16681 tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg tttattcagg
    16741 gctatacggc gctgcttaac ccccattatc tggatgcatc acttctggta ttcgttgaga
    16801 ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct gtacaaaaac
    16861 ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg ttgaaaacac
    16921 gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg cgtctgcctg
    16981 gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt aatcgtctgg
    17041 ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat tacactcctg
    17101 ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt tagcatcggg
    17161 caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg aagacaaaga
    17221 agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcattgc tgatccttat
    17281 tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt cggaccccag
    17341 ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc ccggtgcgtg
    17401 gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc ccgtcattat
    17461 tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca ttgattattt
    17521 tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct gtggtctggc
    17581 ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg gcagcttact
    17641 aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc tgctctgcgt
    17701 ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg ctgaaaaact
    17761 cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc gcgatgatac
    17821 ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa atcacggcaa
    17881 acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc gtaaacggtt
    17941 ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt tctccggtaa
    18001 gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg ccgacccgga
    18061 cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat acgatccatt
    18121 attaaacagt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta ccacggcgac
    18181 acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg cctctgttga
    18241 tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac aaacgggaga
    18301 gccggttatt gctcctgcac cggaaggtta cccacagcag tcacaatatg cgcagcctgc
    18361 agtgcaatat aatgagccgc tgcaacaacc agtacagccg cagcagccgt attatgcacc
    18421 tgcagctgaa caacctgcgc aacagccgta ttatgcccct gcgccagaac aaccggtggc
    18481 aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt ctacatacca
    18541 gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac agccgcaacc
    18601 cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa aacccgcgcg
    18661 tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac gtgaacaact
    18721 tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga tcaaatcttc
    18781 gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg ctgtttctcc
    18841 gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa ccgttgccgc
    18901 gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag aggggattgg
    18961 cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat tggcgtctta
    19021 cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag cccagcgcaa
    19081 tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc agcaggatga
    19141 actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt atcaacatga
    19201 tgcgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg ctcgtcagtt
    19261 tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga atccgttctc
    19321 gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc cacacgaacc
    19381 gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg caccacagca
    19441 gcagtatcag cagccacaac agccggttgc gccacagcag cagtatcagc agccacaaca
    19501 gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc cacagcagca
    19561 atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcaacagc cgcaacaacc
    19621 ggttgcaccg cagccgcagt atcagcagcc acaacagcca gttgcgccac agccgcagga
    19681 taccctgctt catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc acaaaccgac
    19741 gacgccactg ccttctctgg atttgctgac accgccgccg agcgaagtgg agccggtaga
    19801 tacctttgcg cttgaacaaa tggcgcgtct ggtggaagcg cgtctggctg atttccgtat
    19861 taaagccgat gtcgttaatt actctccggg gccagttatc actcgctttg aacttaacct
    19921 ggctcctggc gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg cccgttcact
    19981 ttcgacggtg gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg taggtctgga
    20041 gttaccaaat aaaaaacgac aaaccgttta tctgcgcgaa gttttggata acgccaaatt
    20101 ccgcgataat ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg gtgagccggt
    20161 ggttgccgat ctggcgaaaa tgccgcactt gttggttgcg gggactaccg gttccggtaa
    20221 atctgtcggt gtgaacgcga tgatcctgag catgctttat aaagcacagc cagaagatgt
    20281 gcgtttcatc atgatcgacc cgaaaatgct ggagctttcg gtttatgaag gcattccgca
    20341 cctgttaacg gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc gctggtgtgt
    20401 taacgagatg gagcgtcggt ataaactgat gtctgcgctg ggtgtgcgta atctggcggg
    20461 ttataacgaa aaaattgctg aagccgatcg catgatgcgt ccgattccag acccgtactg
    20521 gaagccgggt gacagtatgg atgcccagca tccggtgctg aaaaaagaac catacattgt
    20581 cgtgttggtt gacgaatttg ccgacctgat gatgacggta ggtaaaaaag tggaagagct
    20641 gatagctcgt ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac tggcaactca
    20701 gcgtccatcg gttgatgtta ttactggtct gattaaagcg aatattccga cccgtatcgc
    20761 ctttaccgta tccagtaaga ttgactcacg taccattctt gatcaggctg gcgcggaatc
    20821 actgctgggt atgggggata tgctctactc tgggccgaac tccacgttgc cggtacgtgt
    20881 ccatggtgct tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact ggaaagcgcg
    20941 tggtcgccca cagtatgttg atggcatcac ctccgacagc gaaagcgaag gtggtgcggg
    21001 tggtttcgat ggcgctgaag aactggatcc gttgttcgat caggcggtgc agtttgtcac
    21061 tgaaaaacgc aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg gttataaccg
    21121 tgcagcgcgt attatcgaac agatggaagc gcaggggatt gtcagcgaac aggggcacaa
    21181 cggtaatcgt gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt gccggataag
    21241 gcgcggtagc gtcgcatccg gcactctatc aactgaaaat tcagtatttt cttctttcct
    21301 caagctgatt attagcctgg aatagagagt agagggaact cccgatcggg agtgacgtaa
    21361 tttgaggaat aatgatgaaa aaaattgcta tcacctgtgc attactctca agcttagtag
    21421 caagcagcgt ttgggctgat gccgcaagcg atctgaaaag ccgcctggat aaagtcagca
    21481 gcttccacgc cagcttcaca caaaaagtga ctgacggtag cggcgcggcg gtgcaggaag
    21541 gtcagggcga tctgtgggtg aaacgtccaa acttattcaa ctggcatatg acacaacctg
    21601 atgaaagcat tctggtttct gacggtaaaa cactgtggtt ctataacccg ttcgttgagc
    21661 aagctacggc aacctggctg aaagatgcca ccggtaatac gccgtttatg ctgattgccc
    21721 gcaaccagtc cagcgactgg cagcagtaca atatcaaaca gaatggcgat gactttgtcc
    21781 tgacgccgaa agccagcaat ggcaatctga agcagttcac cattaacgtg ggacgtgatg
    21841 gcacaatcca tcagtttagc gcggtggagc aggacgatca gcgcagcagt tatcagctga
    21901 aatcccagca aaatggggct gtggatgcag cgaaatttac cttcaccccg ccgcaaggcg
    21961 tcacggtaga tgatcaacgt aagtagaggc acctgagtga gcaatctgtc gctcgatttt
    22021 tcggataata cttttcaacc tctggccgcg cgtatgcggc cagaaaattt agcacagtat
    22081 atcggtcagc aacatttgct ggctgcgggg aagccgttgc cgcgcgctat cgaagccggg
    22141 catttgcatt ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac cctcgctgaa
    22201 gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac ctctggcgtg
    22261 aaagagattc gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg tcgccgcact
    22321 attctttttg ttgacgaagt tcaccgtttc aacaaaagcc agcaggatgc atttctgcca
    22381 catattgaag acggcaccat cacttttatt ggcgcaacca ctgaaaaccc gtcgtttgag
    22441 cttaattcgg cactgctttc ccgtgcccgt gtttatctgt tgaaatccct gagtacagag
    22501 gatattgagc aagtactaac tcaggcgatg gaagacaaaa cccgtggcta tggtggtcag
    22561 gatattgttc tgccagatga tacacgacgt gccattgcag aactggtgaa tggcgacgcg
    22621 cgccgggcgt taaatacgct ggaaatgatg gcggatatgg ccgaagtcta tgatagcggt
    22681 aagcgggtcc tgaagcctga attactgacc gaaatcgccg gtgaacgtag cgcccgcttt
    22741 gataacaaag gcgatcgctt ttacgatcta atttccgcac tgcataagtc ggtacgtggt
    22801 agcgcacccg atgcggcgct gtactggtat gcgcgaatta ttaccgctgg tggcgatccg
    22861 ttatatgtcg cgcgtcgctg tctggcgatt gcgtctgaag acgtcggtaa tgccgatcca
    22921 cgggcgatgc aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg cccggcggaa
    22981 ggtgaacgcg ccattgctca ggcgattgtt tacctggcct gcgcgccaaa aagcaacgct
    23041 gtctacactg cgtttaaagc cgcgctggcc gatgctcgcg aacgcccgga ttatgacgtg
    23101 ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta cgggcaggaa
    23161 tatcgttatg ctcatgatga agcaaacgct tatgctgccg gtgaggttta cttcccgccg
    23221 gaaatagcac aaacacgcta ttatttcccg acaaacaggg gccttgaagg caagattggt
    23281 gaaaagctcg cctggctggc tgaacaggat caaaatagcc ccataaaacg ctaccgttaa
    23341 tgttatcgtt gcggtaatgt tgttactgta tccctgtggt cgcaggctgt ggccacatct
    23401 cccatttaat tcgataagca caggataagc atgctcgatc ccaatctgct gcgtaatgag
    23461 ccagacgcag tcgctgaaaa actggcacgc cggggcttta agctggatgt agataagctg
    23521 ggcgctcttg aagagcgtcg taaagtattg caggtcaaaa cggaaaacct gcaagcggag
    23581 cgtaactccc gatcgaaatc cattggccag gcgaaagcgc gcggggaaga tatcgagcct
    23641 ttacgtctgg aagtgaacaa actgggcgaa gagctggatg cagcaaaagc cgagctggat
    23701 gctttacagg ctgaaattcg cgatatcgcg ctgaccatcc ctaacctgcc tgcagatgaa
    23761 gtgccggtag gtaaagacga aaatgacaac gttgaagtca gccgctgggg taccccgcgt
    23821 gagtttgact ttgaagttcg tgaccacgtg acgctgggtg aaatgcactc tggcctcgac
    23881 tttgcagctg cagttaagct gactggttcc cgctttgtgg taatgaaagg gcagattgct
    23941 cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc ataccgaaca gcatggctac
    24001 agtgagaact atgttccgta cctggttaac caggacacgc tgtacggtac gggtcagctg
    24061 ccgaaatttg ctggcgatct gttccatact cgtccgctgg aagaagaagc agacaccagt
    24121 aactatgcgc tgatcccaac ggcagaagtt ccgctgacta acctggtgcg cggtgaaatc
    24181 atcgatgaag atgatctgcc aattaagatg accgcccaca ccccatgctt ccgttctgaa
    24241 gccggttcat atggtcgtga cacccgtggt ctgatccgta tgcaccagtt cgacaaagtt
    24301 gaaatggtgc agatcgtgcg cccagaagac tcaatggcgg cgctggaaga gatgactggt
    24361 catgcagaaa aagtcctgca gttgctgggc ctgccgtacc gtaaaatcat cctttgcact
    24421 ggcgacatgg gctttggcgc ttgcaaaact tacgacctgg aagtatggat cccggcacag
    24481 aacacctacc gtgagatctc ttcctgctcc aacgtttggg atttccaggc acgtcgtatg
    24541 caggcacgtt gccgcagcaa gtcggacaag aaaacccgtc tggttcatac cctgaacggt
    24601 tctggtctgg ctgttggtcg tacgctggtt gcagtaatgg aaaactatca gcaggctgat
    24661 ggtcgtattg aagtaccaga agttctgcgt ccgtatatga acggactgga atatattggc
    24721 taatacccaa tttttctgaa tctaaaaagc gcctttaggg cgcttttttt gtctcccttt
    24781 gataccgaac aataattact cctcacttac acgtaatact actttcgagt gaaaatctac
    24841 ctatctcttt gattttcaaa ttattctatg tatacaagcc tatatagcga gctgctatag
    24901 aaataattac acaatacggt ttgttactgg aatcaatcgt gatcaagctt gagtgagcca
    24961 ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggagagtcgc cgtggtttgg
    25021 taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca ttacctttta
    25081 gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa aaggttatct
    25141 ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac gtcgtggacg
    25201 gtgaaatcaa atatgtcgaa acgacaatac cggcgatgac aattacgacg gcctacacta
    25261 ggttcgcgcc tgcctgcgtg ggcgttccat gcgtcaccgt gtctacaatc cggaccgcct
    25321 gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc gcattagctg
    25381 ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag agtacggcaa
    25441 cgagtctatc tatctgaact atggcaccgg tacgctgggc ggcaccatga cccgctcctg
    25501 gccgccggga aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt atcttaacca
    25561 ttacggtgac tactcttccg cgcaaattgc ggaaggtttg aaccatacct acggcggctg
    25621 ggcagatggt aacagcccgt cggatatcga aaacagtaaa ttggtagtgc tgtttggtaa
    25681 taacccaggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg aacaggcacg
    25741 ccagaaatct aatgctcgca tgatcatcat cgatccgcgc tataccgaca ccggtgccgg
    25801 gcgcgaagat gagtggatcc ctattcgtcc aggaacagat gccgcactgg ttaacggtct
    25861 ggcgtacgtc atgatcacta aaaacctggt ggatcaggca ttcctcgata aatattgcgt
    25921 tggctatgac gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt ataaagctta
    25981 tattctgggt gaagggccag atggcgtggc taaaacaccg gaatgggcct cgcaaatcac
    26041 cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta ccaaaccggc
    26101 gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg caacccgtgc
    26161 tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aatggaggga acagcggcgc
    26221 gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accttggaaa acccgatcca
    26281 gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg aaatgacggc
    26341 gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa tgatctggaa
    26401 ctatgccggt aactgcctga ttaaccagca ttctgaaatc aaccgtaccc atgaaatcct
    26461 ccaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga cctcatcggc
    26521 gaaatatgct gacatcctgc tgcctgactg caccgcttcc gaacagatgg actttgcact
    26581 ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga ttaaaccgcg
    26641 ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc ttggcgttga
    26701 gcaacagttt actgaaggcc gtacccagga agagtggatg cggcatctgt atgcccagtc
    26761 gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgcaagcagg ggatctttaa
    26821 aaagcgcgac ccacaagggc atcacgttgc ttataaagcc ttccgtgaag atccgcaggc
    26881 aaacccactg actacgccat cgggcaaaat tgagatttat tcgcaggcgc tggctgacat
    26941 tgccgctacc tgggaattgc cagaaggcga tgtgatcgat ccactgccga tctacacgcc
    27001 gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc ttacaggttt
    27061 ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga aagcggcttg
    27121 ccgtcaggaa atgtggatca acccgcttga tgcccaaaaa cgcggtatcc acaacggcga
    27181 taaagtcagg atctttaacg atcgtggtga ggttcatatt gaggcgaaag tgacgccacg
    27241 aatgatgccg ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg atgcaaaacg
    27301 tgtcgataag ggcggttgta tcaacgtact gaccactcaa cgtccgtctc ctctcgctaa
    27361 ggggaatccg tcacatacaa accttgttca ggttgaaaag gtgtaaggag taaccgatga
    27421 caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa acctgcgagc
    27481 tggcttgtaa agactacaaa gatttgacgc cagaagtcag tttccgccgc atttatgaat
    27541 atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg tttgcctact
    27601 atctgtcgat ttcgtgcaac cactgcgaag atccggcttg tactaaagtc tgcccgagcg
    27661 gtgcgatgca taaacgcgaa gatggttttg ttgtggtcga tgaagatgta tgcattggct
    27721 gccgttactg tcatatggct tgcccgtatg gcgcaccgca atataacgaa acgaaaggcc
    27781 atatgaccaa atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag ccgatctgtg
    27841 ttgaatcttg cccgctgcgg gcgctggatt tcggccctat cgacgagctg cgtaaaaaac
    27901 atggcgatct ggcggccgtt gcgccgttgc tgcgagctca ctttaccaaa ccgaatattg
    27961 tgatcaaacc caatgccaat agccgcccga ccggggatac cactggctat ctggcaaacc
    28021 cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat cttcacggtc
    28081 ttcgggcaat gtgtggcggg tggttttatc gttctggctt tggcgctgct caaaggcgac
    28141 ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt ttggcttatg ggtactgatg
    28201 ggcattggct ttatcgcctc tatgctccat cttggttcac cgatgcgcgc ctttaactcg
    28261 ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc gatctttttt
    28321 gctgtaggcg gcatcggctg gttgctggca atgctgaaaa agctgtcacc ggcattgcgt
    28381 acgctatggc tggtggtgac gatggttctt ggcgtcgtct ttgtctggat gatggtgcgt
    28441 gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc gatgggctgg
    28501 gcgatgcgtc tgctgccagc catttctgta ctggcactgg tggtgagtgg cgtgatgtca
    28561 gtgatgcagg gcacagagct ggcaactatt catagttctg tgcagcaggc cgcagcgctg
    28621 gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt tgccctgtgc
    28681 ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact atccgtctca
    28741 ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg tttgcatatg
    28801 accgtgggga tggccgtcgc aagctaatca gtaacaaccg gggtttcggc cccggttctc
    28861 ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata atcaggaata
    28921 tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcacg gtgcagctcg
    28981 caagccacgc cgaaccatgt catcaattgc gcaccagctt gcgacatgcg atcccacgcc
    29041 gaatgccggg taatttcatt aaaggtccct gatgcatcag tcacgacaaa gacatcaaac
    29101 ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac cacaccggca
    29161 ataattaact gttttttacc tgtcgctttg actgctttta caaaatcttc gttatcccag
    29221 gcgttaatat tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc tttcagttct
    29281 agaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt tggtaagttg
    29341 aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc gggttcgata
    29401 tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc agcatcattt
    29461 ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc agtggtacgt
    29521 ctgaaatcgg tcagacgcga tgcattgctc tgaaagcata gacgggaaat atgagtttgc
    29581 tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag atcaataaaa
    29641 acaatcagat gagattaatc atctcttcag cattagcgtt atgaatgagt aatataaatc
    29701 gataataccg ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt tttggcggtg
    29761 gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca cgtatacccg
    29821 gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgatcg cggtgttaaa
    29881 tacactcgtg ccgctttggc tcgcccagga acacatgtcc acatggcagg taggcgttgt
    29941 cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt atgtcattaa
    30001 acgcattggc tttaaccgca gctattatct gtcctccttc atttttgctg ctggctgtgc
    30061 cggccttggc ctgatgattg gtttctggag ctggttggcg tggcgttttg tcgcgggcgt
    30121 cggctgtgcc atgatttggg tggttgttga gagcgcgctg atgtgcagtg ggacgtcacg
    30181 taaccgtggg cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa cgtttttagg
    30241 ccagttactg gtcagcaaag tctcaaccga gctgatgagc gtattgccgt gggttacagg
    30301 tttgacattg gcagggatct taccgctgtt gtttacgcgt gtcctgaatc agcaggctga
    30361 aaaccatgat tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc ggcttggcgt
    30421 gaatggctgc attatctcag gaatcgttct gggatctcta tatggcctga tgcctctgta
    30481 cctcaatcac aaaggggtga gcaatgccag tattggtttc tggatggcgg tactggtcag
    30541 tgcgggtatc ctcggacaat ggccgattgg acgtctggcg gataagtttg gtcgactgct
    30601 ggttttgcgt gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc ttagccaggc
    30661 ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc tttacgctat atccggtggc
    30721 gatggcctgg gcttgtgaga aagttgaaca tcatcaactg gtggcgatga accaagcctt
    30781 actgttgagc tataccgtgg gaagtctgct tggcccgtca tttagcgcta tgctaatgca
    30841 gaatttctcc gataatttat tgtttatcat gatcgccagc gtatcgttta tctatttgct
    30901 gatgctgctt cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt aaatgaattc
    30961 aagcaaagtg tgaacttact gtttcacact ctgctttttt gtttcttcta tctgacttgc
    31021 ttttttccaa attttattcg tttaaaaata aaatgtgcag caggttataa ttttgcattt
    31081 cgctatttcc gcacttctta tttgccgcgc ataatccctc gttttacaga tgccccttta
    31141 attttggcga aggatttgtc tatggctggg aatgttcag
//
LOCUS       DAJTYM010000041        31034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTYM010000041 DAJTYM010000000
VERSION     DAJTYM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(135..341)
                     /locus_tag="OO575_001914"
     CDS             complement(135..341)
                     /locus_tag="OO575_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6664903.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            654..743
                     /gene="kdpF"
                     /locus_tag="OO575_001915"
     CDS             654..743
                     /gene="kdpF"
                     /locus_tag="OO575_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6664904.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            743..2416
                     /gene="kdpA"
                     /locus_tag="OO575_001916"
     CDS             743..2416
                     /gene="kdpA"
                     /locus_tag="OO575_001916"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6664905.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2439..4486
                     /gene="kdpB"
                     /locus_tag="OO575_001917"
                     /pseudo
     CDS             2439..4486
                     /gene="kdpB"
                     /locus_tag="OO575_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4495..5067
                     /gene="kdpC"
                     /locus_tag="OO575_001918"
     CDS             4495..5067
                     /gene="kdpC"
                     /locus_tag="OO575_001918"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6664906.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5060..7740
                     /gene="kdpD"
                     /locus_tag="OO575_001919"
                     /pseudo
     CDS             5060..7740
                     /gene="kdpD"
                     /locus_tag="OO575_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7737..8422
                     /gene="kdpE"
                     /locus_tag="OO575_001920"
                     /pseudo
     CDS             7737..8422
                     /gene="kdpE"
                     /locus_tag="OO575_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8697..8801
                     /locus_tag="OO575_001921"
     CDS             8697..8801
                     /locus_tag="OO575_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6664907.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            9112..11309
                     /gene="speF"
                     /locus_tag="OO575_001922"
                     /pseudo
     CDS             9112..11309
                     /gene="speF"
                     /locus_tag="OO575_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11306..12625
                     /gene="potE"
                     /locus_tag="OO575_001923"
     CDS             11306..12625
                     /gene="potE"
                     /locus_tag="OO575_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6664908.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12681..14321)
                     /gene="pgm"
                     /locus_tag="OO575_001924"
     CDS             complement(12681..14321)
                     /gene="pgm"
                     /locus_tag="OO575_001924"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6664909.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14347..14892)
                     /gene="seqA"
                     /locus_tag="OO575_001925"
     CDS             complement(14347..14892)
                     /gene="seqA"
                     /locus_tag="OO575_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6664910.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15077..15841
                     /gene="ybfF"
                     /locus_tag="OO575_001926"
     CDS             15077..15841
                     /gene="ybfF"
                     /locus_tag="OO575_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6664911.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15981..16274
                     /gene="ybfE"
                     /locus_tag="OO575_001927"
     CDS             15981..16274
                     /gene="ybfE"
                     /locus_tag="OO575_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6664912.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16414..16944
                     /gene="fldA"
                     /locus_tag="OO575_001928"
     CDS             16414..16944
                     /gene="fldA"
                     /locus_tag="OO575_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6664913.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17154..17240
                     /gene="uof"
                     /locus_tag="OO575_001929"
     CDS             17154..17240
                     /gene="uof"
                     /locus_tag="OO575_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6664914.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17233..17679
                     /gene="fur"
                     /locus_tag="OO575_001930"
     CDS             17233..17679
                     /gene="fur"
                     /locus_tag="OO575_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6664915.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17763..18089)
                     /locus_tag="OO575_001931"
     CDS             complement(17763..18089)
                     /locus_tag="OO575_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664916.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18126..19532)
                     /gene="chiP"
                     /locus_tag="OO575_001932"
     CDS             complement(18126..19532)
                     /gene="chiP"
                     /locus_tag="OO575_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6664917.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20111..21775)
                     /gene="glnS"
                     /locus_tag="OO575_001933"
     CDS             complement(20111..21775)
                     /gene="glnS"
                     /locus_tag="OO575_001933"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6664918.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(21978..23924)
                     /gene="nagE"
                     /locus_tag="OO575_001934"
     CDS             complement(21978..23924)
                     /gene="nagE"
                     /locus_tag="OO575_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6664919.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24257..25057
                     /gene="nagB"
                     /locus_tag="OO575_001935"
     CDS             24257..25057
                     /gene="nagB"
                     /locus_tag="OO575_001935"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6664920.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25117..26265
                     /gene="nagA"
                     /locus_tag="OO575_001936"
     CDS             25117..26265
                     /gene="nagA"
                     /locus_tag="OO575_001936"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6664921.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26274..27494
                     /gene="nagC"
                     /locus_tag="OO575_001937"
                     /gene_synonym="nagR"
     CDS             26274..27494
                     /gene="nagC"
                     /locus_tag="OO575_001937"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6664922.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27542..28294
                     /gene="nagD"
                     /locus_tag="OO575_001938"
                     /pseudo
     CDS             27542..28294
                     /gene="nagD"
                     /locus_tag="OO575_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28691..30355
                     /gene="asnB"
                     /locus_tag="OO575_001939"
     CDS             28691..30355
                     /gene="asnB"
                     /locus_tag="OO575_001939"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6664923.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            30734..30810
                     /locus_tag="OO575_001940"
     tRNA            30734..30810
                     /locus_tag="OO575_001940"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30768..30770,aa:Met,seq:cat)
     gene            30819..30903
                     /locus_tag="OO575_001941"
     tRNA            30819..30903
                     /locus_tag="OO575_001941"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30853..30855,aa:Leu,seq:tag)
     gene            30927..31001
                     /locus_tag="OO575_001942"
     tRNA            30927..31001
                     /locus_tag="OO575_001942"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30959..30961,aa:Gln,seq:ttg)
BASE COUNT         7369 a         7869 c         8099 g         7697 t
ORIGIN      
        1 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
       61 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      121 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      181 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      241 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      301 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      361 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      421 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      481 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      541 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      601 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      661 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
      721 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
      781 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
      841 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
      901 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
      961 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1021 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1081 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1141 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1201 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1261 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1321 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1381 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1441 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1501 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1561 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1621 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1681 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     1741 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     1801 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     1861 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     1921 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     1981 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2041 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2101 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2161 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2221 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2281 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2341 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2401 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2461 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2521 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2581 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2641 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     2701 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     2761 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     2821 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     2881 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     2941 aatccggcgg cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg
     3001 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3061 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3121 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3181 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3241 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3301 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3361 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3421 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3481 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3541 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3601 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3661 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     3721 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     3781 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     3841 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     3901 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     3961 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4021 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4081 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4141 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4201 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4261 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4321 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4381 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4441 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4501 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4561 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4621 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4681 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     4741 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     4801 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     4861 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     4921 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     4981 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5041 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5101 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5161 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5221 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5281 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5341 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5401 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5461 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5521 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5581 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5641 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     5701 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     5761 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     5821 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcacggcggc gcggctgacg
     5881 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     5941 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6001 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6061 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6121 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6181 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6241 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6301 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6361 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6421 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6481 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6541 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6601 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6661 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     6721 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     6781 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     6841 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     6901 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     6961 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7021 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7081 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7141 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7201 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7261 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7321 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7381 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7441 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7501 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7561 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7621 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7681 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     7741 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     7801 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     7861 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     7921 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     7981 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8041 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8101 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8161 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8221 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8281 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8341 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8401 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8461 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8521 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8581 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8641 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     8701 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttaccc
     8761 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     8821 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     8881 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     8941 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9001 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9061 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9121 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9181 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9241 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9301 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9361 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9421 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9481 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9541 aatcagttcg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9601 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9661 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
     9721 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
     9781 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
     9841 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
     9901 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
     9961 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10021 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10081 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10141 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10201 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10261 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10321 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10381 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10441 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10501 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10561 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10621 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10681 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    10741 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    10801 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    10861 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    10921 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    10981 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11041 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11101 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11161 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11221 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11281 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11341 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11401 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11461 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11521 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11581 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11641 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    11701 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    11761 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    11821 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    11881 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    11941 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12001 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12061 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12121 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12181 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12241 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12301 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12361 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12421 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12481 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12541 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12601 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12661 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    12721 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    12781 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    12841 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    12901 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    12961 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13021 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13081 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13141 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13201 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13261 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13321 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13381 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13441 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13501 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13561 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13621 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc
    13681 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    13741 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct
    13801 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    13861 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    13921 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    13981 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14041 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14101 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14161 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14221 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14281 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14341 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14401 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14461 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14521 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14581 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14641 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    14701 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    14761 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    14821 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    14881 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    14941 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15001 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15061 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15121 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15181 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15241 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15301 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15361 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15421 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15481 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15541 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15601 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15661 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    15721 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    15781 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    15841 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    15901 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    15961 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16021 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16081 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16141 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16201 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16261 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16321 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16381 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16441 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16501 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16561 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16621 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16681 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    16741 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    16801 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    16861 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    16921 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    16981 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17041 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17101 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17161 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17221 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17281 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17341 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17401 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17461 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17521 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17581 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17641 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    17701 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    17761 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    17821 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    17881 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    17941 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18001 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18061 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18121 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18181 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18241 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18301 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18361 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18421 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18481 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18541 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18601 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18661 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    18721 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    18781 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    18841 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    18901 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    18961 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19021 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19081 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19141 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19201 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19261 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19321 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19381 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19441 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19501 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19561 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19621 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19681 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    19741 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    19801 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    19861 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    19921 tttgctattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa
    19981 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
    20041 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
    20101 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
    20161 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
    20221 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
    20281 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
    20341 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
    20401 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
    20461 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
    20521 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
    20581 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
    20641 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
    20701 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
    20761 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
    20821 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
    20881 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
    20941 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
    21001 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
    21061 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
    21121 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
    21181 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
    21241 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
    21301 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
    21361 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
    21421 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
    21481 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
    21541 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
    21601 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
    21661 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
    21721 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
    21781 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
    21841 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
    21901 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
    21961 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
    22021 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
    22081 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
    22141 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
    22201 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
    22261 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
    22321 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
    22381 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
    22441 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
    22501 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
    22561 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
    22621 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
    22681 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
    22741 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
    22801 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
    22861 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
    22921 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
    22981 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
    23041 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
    23101 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
    23161 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
    23221 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
    23281 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
    23341 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
    23401 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
    23461 accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
    23521 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
    23581 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
    23641 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
    23701 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
    23761 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
    23821 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
    23881 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
    23941 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
    24001 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
    24061 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
    24121 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc
    24181 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
    24241 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
    24301 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
    24361 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
    24421 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
    24481 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
    24541 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
    24601 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
    24661 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
    24721 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
    24781 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
    24841 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
    24901 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
    24961 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
    25021 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
    25081 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg
    25141 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
    25201 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
    25261 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
    25321 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
    25381 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
    25441 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
    25501 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
    25561 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
    25621 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
    25681 attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
    25741 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
    25801 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
    25861 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
    25921 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
    25981 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
    26041 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
    26101 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
    26161 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
    26221 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
    26281 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
    26341 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
    26401 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
    26461 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
    26521 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
    26581 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
    26641 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
    26701 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
    26761 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
    26821 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
    26881 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
    26941 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
    27001 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
    27061 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
    27121 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
    27181 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
    27241 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
    27301 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
    27361 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
    27421 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
    27481 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
    27541 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
    27601 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
    27661 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
    27721 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
    27781 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
    27841 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
    27901 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
    27961 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
    28021 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
    28081 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
    28141 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
    28201 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
    28261 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
    28321 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
    28381 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
    28441 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
    28501 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
    28561 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
    28621 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
    28681 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
    28741 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
    28801 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
    28861 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
    28921 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
    28981 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
    29041 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
    29101 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
    29161 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
    29221 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
    29281 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
    29341 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
    29401 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
    29461 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
    29521 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
    29581 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
    29641 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
    29701 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
    29761 ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa
    29821 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
    29881 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
    29941 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    30001 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    30061 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    30121 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    30181 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    30241 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    30301 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    30361 gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    30421 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    30481 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    30541 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    30601 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    30661 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    30721 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    30781 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    30841 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    30901 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    30961 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    31021 tcaccgcccg gtta
//
LOCUS       DAJTYM010000042        30817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTYM010000042 DAJTYM010000000
VERSION     DAJTYM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..723
                     /locus_tag="OO575_001943"
     CDS             <1..723
                     /locus_tag="OO575_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HCR6664924.1"
                     /translation="EDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWA
                     PHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLS
                     YITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIP
                     VLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRSFYGRHAVEVLY
                     QNQGALQRLLQLLEPYLPPKTH"
     gene            731..1210
                     /gene="coaD"
                     /locus_tag="OO575_001944"
     CDS             731..1210
                     /gene="coaD"
                     /locus_tag="OO575_001944"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6664925.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1249..2058)
                     /gene="mutM"
                     /locus_tag="OO575_001945"
     CDS             complement(1249..2058)
                     /gene="mutM"
                     /locus_tag="OO575_001945"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6664926.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2156..2323)
                     /gene="rpmG"
                     /locus_tag="OO575_001946"
     CDS             complement(2156..2323)
                     /gene="rpmG"
                     /locus_tag="OO575_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6664927.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2344..2580)
                     /gene="rpmB"
                     /locus_tag="OO575_001947"
     CDS             complement(2344..2580)
                     /gene="rpmB"
                     /locus_tag="OO575_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6664928.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(2797..3465)
                     /gene="radC"
                     /locus_tag="OO575_001948"
     CDS             complement(2797..3465)
                     /gene="radC"
                     /locus_tag="OO575_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6664929.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            3637..4857
                     /gene="coaBC"
                     /locus_tag="OO575_001949"
     CDS             3637..4857
                     /gene="coaBC"
                     /locus_tag="OO575_001949"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6664930.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            4835..5293
                     /gene="dut"
                     /locus_tag="OO575_001950"
     CDS             4835..5293
                     /gene="dut"
                     /locus_tag="OO575_001950"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6664931.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            5400..5996
                     /gene="slmA"
                     /locus_tag="OO575_001951"
     CDS             5400..5996
                     /gene="slmA"
                     /locus_tag="OO575_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6664932.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6033..6674)
                     /gene="pyrE"
                     /locus_tag="OO575_001952"
     CDS             complement(6033..6674)
                     /gene="pyrE"
                     /locus_tag="OO575_001952"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6664933.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6740..7456)
                     /gene="rph"
                     /locus_tag="OO575_001953"
     CDS             complement(6740..7456)
                     /gene="rph"
                     /locus_tag="OO575_001953"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6664934.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            7583..8446
                     /locus_tag="OO575_001954"
     CDS             7583..8446
                     /locus_tag="OO575_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6664935.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            8667..9491
                     /gene="dinD"
                     /locus_tag="OO575_001955"
     CDS             8667..9491
                     /gene="dinD"
                     /locus_tag="OO575_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6664936.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            9782..10399
                     /locus_tag="OO575_001956"
     CDS             9782..10399
                     /locus_tag="OO575_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6664937.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10396..12078)
                     /gene="ligB"
                     /locus_tag="OO575_001957"
     CDS             complement(10396..12078)
                     /gene="ligB"
                     /locus_tag="OO575_001957"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6664938.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12336..12959
                     /gene="gmk"
                     /locus_tag="OO575_001958"
     CDS             12336..12959
                     /gene="gmk"
                     /locus_tag="OO575_001958"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6664939.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13014..13289
                     /gene="rpoZ"
                     /locus_tag="OO575_001959"
     CDS             13014..13289
                     /gene="rpoZ"
                     /locus_tag="OO575_001959"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6664940.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13308..15416
                     /gene="spoT"
                     /locus_tag="OO575_001960"
     CDS             13308..15416
                     /gene="spoT"
                     /locus_tag="OO575_001960"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6664941.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            15423..16112
                     /gene="trmH"
                     /locus_tag="OO575_001961"
     CDS             15423..16112
                     /gene="trmH"
                     /locus_tag="OO575_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6664942.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16118..18199
                     /gene="recG"
                     /locus_tag="OO575_001962"
     CDS             16118..18199
                     /gene="recG"
                     /locus_tag="OO575_001962"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6664943.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18233..19438)
                     /gene="gltS"
                     /locus_tag="OO575_001963"
     CDS             complement(18233..19438)
                     /gene="gltS"
                     /locus_tag="OO575_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6664944.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            19718..21109
                     /gene="xanP"
                     /locus_tag="OO575_001964"
     CDS             19718..21109
                     /gene="xanP"
                     /locus_tag="OO575_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6664945.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21230..22939
                     /locus_tag="OO575_001965"
     CDS             21230..22939
                     /locus_tag="OO575_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6664946.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(22992..25310)
                     /gene="yicI"
                     /locus_tag="OO575_001966"
     CDS             complement(22992..25310)
                     /gene="yicI"
                     /locus_tag="OO575_001966"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6664947.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25320..26703)
                     /locus_tag="OO575_001967"
                     /pseudo
     CDS             complement(25320..26703)
                     /locus_tag="OO575_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            26996..27090
                     /locus_tag="OO575_001968"
     tRNA            26996..27090
                     /locus_tag="OO575_001968"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27030..27032,aa:Sec,seq:tca)
     gene            27362..28573
                     /locus_tag="OO575_001969"
     CDS             27362..28573
                     /locus_tag="OO575_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6664948.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            28985..30028
                     /locus_tag="OO575_001970"
     CDS             28985..30028
                     /locus_tag="OO575_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6664949.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            30543..>30817
                     /locus_tag="OO575_001971"
     CDS             30543..>30817
                     /locus_tag="OO575_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664950.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVT"
BASE COUNT         7737 a         8110 c         7648 g         7322 t
ORIGIN      
        1 gaagatggtg cacgttttgt ggcgctgggc gcaaaaaata accaggtaac cgttaccggt
       61 agcctgaaat tcgatatttc tgtaactccg cagttggctg ctaaagccgt gacgctacgc
      121 cgccaatggg caccacaccg cccggtatgg attgccacca gcactcacga aggtgaagag
      181 agcgtggtca ttgccgcaca tcaggcattg ttacagcaat tcccgaattt attgctcatc
      241 ctggtacccc gtcatccaga acgcttcccg gatgcgatta accttgtccg ccaggctgga
      301 ctaagctata tcacacgctc ttcaggggaa gtcccctcca ccagcacgca ggttgtggtt
      361 ggcgatacga tgggcgagtt gatgttactg tacggcattg ccgatctcgc ctttgttggc
      421 ggttcactgg ttgaacgtgg tgggcataat ccgctggaag ctgccgcaca cgctattccg
      481 gtattgatgg ggccgcatac ttttaacttt aaagacattt gcgcgcggct ggagcaggca
      541 agcgggctta ttaccgttac cgatgccact acgcttgcaa aagaggtttc ctctttactc
      601 accgacgccg tttaccgtag tttctatggc cgtcatgccg ttgaagtact gtatcaaaac
      661 cagggcgcgc tacagcgtct gcttcaactg ctggaacctt acctgccacc gaaaacgcat
      721 tgaggttgtt atgcaaaaac gggcgattta tccgggtact ttcgatccca ttaccaatgg
      781 tcatatcgat atcgtgacgc gcgccacgca gatgttcgat cacgttattc tggcgattgc
      841 cgccagcccc agtaaaaaac cgatgtttac cctggaagag cgtgtggcac tggcacagca
      901 ggcaaccgcg catctgggga acgtggaagt ggtcgggttt agtgatttga tggcgaactt
      961 cgcccgtaat caacacgcta cggtgctgat tcgtggcctg cgtgcggtgg cggattttga
     1021 atatgaaatg cagctggcgc atatgaatcg ccacttaatg ccggaactgg aaagtgtgtt
     1081 tctgatgccg tcgaaagagt ggtcgtttat ctcttcatcg ttggtgaaag aggtggcgcg
     1141 ccatcagggc gatgtcaccc atttcctgcc ggagaatgtc catcaggcgc tgatggcaaa
     1201 attagcgtag cgtttatgcc ggatggtatg ccatccggct cgcatgaatt acttctggca
     1261 ctgccgacaa taaaacgttg cccgctgcgc atgtttagtc gccacaatcg gcgtaccgca
     1321 cacccgacac ggctcacctt ttcgcccgta aacctgcaat tcctgcgcga aatagcccgg
     1381 tttaccatca ctttgcagaa aatctttcag cgttgtacca ccctgctcaa tcgaacgcag
     1441 caacaccgct ttaatcaccc gagctaacaa ttcacactct gccagcgaca gtgatgacgc
     1501 cagccgatcc ggatggatcc ccgccgcaaa cagtgattcg ctggcataga tattccctac
     1561 ccctaccacc agcttgttat ccatcagcca cggtttaatc gccgtttttt tcttcgcgca
     1621 cttctgatgc agatactcac cattgaaatc gtcgctaagc ggctccggtc caagatgggc
     1681 cagcacatta tgcccttcca gctctttggt ccacagccag gcaccaaagc ggcgcggatc
     1741 ggtgtagcgc agcactttac cgttgctcat caccaaatcc acatggtcat gcttttcagg
     1801 gggaagttct tctggaagga tgcgcaggct gccagacatc cccaaatgaa tgataatcca
     1861 gccctcaggc agctccagca gcagatattt agcccgccgc tgcacgctaa gcactggttg
     1921 atcgcttaat cggtagatct cttctgaaac cggccagcgc aagcgtccgt tgcgcaccac
     1981 cgcatgaaga atggttgcac caacgagatg cggttctatg ccgcgacggc tggtttcaac
     2041 ttcgggtaat tcaggcatag catctccagg aatgaacaga tgcaatcaat atgggggcaa
     2101 gcagataaca aaaaaccccg ccggagcgag gtttttcgtt acatcaaagc gagaattatt
     2161 tgattttcgc ttctttgtag atcacgtgct gacgaacaac tggatcgaat tttttcagtt
     2221 ccagtttttc cggcttagta cgtttgttct tcgtagtggt atagaagtga ccagtaccag
     2281 cagaagaaac cagcttgatt ttctcacgaa tacctttagc catgatttat ttcctctaag
     2341 tacttagtac ttttcgccac gggcacgcag ttcagccaga actgtatcga tgcctttttt
     2401 atcgattaca cgcatacctt tagcagatac gcgcagggtg acaaaacgct tctcgctctc
     2461 aacccagaaa cggtgagagt gcaggttcgg caggaaacgg cgtttagtcg cgttcagtgc
     2521 gtgggaacgg ttgttaccgg tcaccggacg cttgccagta acttggcaga ctcgggacat
     2581 gtctattctc caaaaatcaa attagctcga gcttcgtatg gggtattggc gcctcgtcag
     2641 gctttacagc ccggtcatcg cagttctatg tgaactctcg attgccaggc ccaaatgcca
     2701 aacccgagat tctcaaaggt ggcgtagtat acgctgactc agcgatgtgc tcaagtcccg
     2761 aacagacaaa gatcccgaag gatcgcgcat agcgggttaa atccagccgc gttcggcaaa
     2821 agaaacatac tctccacgcc caatcacgat atggtcgagc acgcgtaaat ccatgaactg
     2881 acaactcttt attatccgtt cagtaatgag tttatccgct ttactgggtt cagcacaacc
     2941 cgaagggtga ttatgtgcaa ggatcagcgc cgaggcgttt atttttatcg cttcgcggat
     3001 aatttcccga ggatggactt caacatggtt tagcgtgccg gaaaaaagac ggctatgcgt
     3061 tataacccgg tgttgtgagt cgagaaagat caccataaag atctcccgct cctcacccgt
     3121 gagctggctt tgtaaaaatt cccgcgtcat ctccgggctg agtaaagggc tttcttcacg
     3181 catccgcacg ttgtagtaac gtcgcgccag ttcagcaatc ccctttaact gggcaaattt
     3241 cgctacgcca attccatgaa caccactaaa ttgttcatat tcagaggtta acaagccata
     3301 aagagagccg aaattctcca gcatctcttt tgccagggtt aatacatctt taccgcgcgt
     3361 tccggtacgc agaaatagcg ccagcagctc gacatccgtt aaggcgctaa taccaaactt
     3421 cagcattttt tcgcgcggca tcaacagctg tgcattgttt ttcaccttca cctcctttgt
     3481 ggtgctcgca tcctgtcata gccgttccgt taaatcgacg gccagttttc aatcctggaa
     3541 agagcctcgc aaagtgaatc tcaggtgaag gcacgacaac aaaagtattg tgataaaatc
     3601 gccaacttct ggtgtcaccc aacaggaaaa atcatcatga gcctggccgg taaaaaaatc
     3661 gttctcggcg ttagcggcgg tattgctgcc tataaaaccc ctgaactggt gcgtcgtttg
     3721 cgcgatcgcg gggccgacgt ccgcgtagcc atgaccgaag cggcaaaagc ctttatcacc
     3781 ccacttagct tgcaggcggt ttctggttat cccgtttccg acagtctgct tgacccggca
     3841 gccgaagccg ctatgggcca tattgagctg ggtaaatggg ctgatttagt gattctcgcc
     3901 cctgccacgg cagatttgat tgcccgtgtt gctgccggaa tggcgaatga cctggtatcg
     3961 acgatttgtc tggctacacc tgcgcctgta gccgtgctcc ccgccatgaa ccagcagatg
     4021 taccgtgccg ctgccacgca gcataattta gaggtgcttg cttcccgtgg tttgctcatc
     4081 tgggggccag acagtggcag tcaggcttgt ggtgatatcg gtcctggacg aatgctcgat
     4141 ccgttaacca ttgtggatat ggcggtggcg catttttcgc ccgtcaacga cctgaaacat
     4201 ctgaacatta tgattaccgc cggtccgacg cgtgaaccgc tcgatccggt gcgttatatc
     4261 tctaatcaca gctccggcaa gatgggtttt gctatcgccg ccgctgctgc ccgtcgtggc
     4321 gcgaacgtca cgctggtatc aggtccagtt tcactaccga cgccaccgtt tgttaaacgt
     4381 gttgatgtga tgaccgcgct ggaaatggaa gccgccgtga atgcttctgt acagcagcaa
     4441 aatattttta tcggttgcgc cgccgtggcg gattatcgcg cagctaccgt ggccccagaa
     4501 aaaatcaaaa agcaggccac gcagggtgat gaattaacaa taaaaatggt taaaaacccc
     4561 gatatcgtcg caggcgttgc cgcactaaaa gaccatcgac cctacgtcgt tggatttgcc
     4621 gccgaaacaa ataatgtgga agaatacgcc cggcaaaaac gtatccgtaa aaaccttgat
     4681 ctgatctgcg cgaacgatgt ttcccagcca actcaaggat ttaacagcga caacaacgca
     4741 ttacaccttt tctggcagga cggagataaa gtcttaccgc ttgagcgcaa agagctcctt
     4801 ggccaattat tactcgacga gatcgtgacc cgttatgatg aaaaaaatcg acgttaagat
     4861 tctggacccg cgcgttagga aggaatttcc gctcccgact tatgccacct ctggctctgc
     4921 cggacttgac ctgcgtgcct gtctcgacga cgccgtagaa ctggctccgg gtgacactac
     4981 gctggttccg accgggctgg cgattcatat tgccgatcct tcactggcgg caatgatgct
     5041 gccgcgctcc ggattgggac ataagcacgg tatcgtgctt ggtaacctgg taggattgat
     5101 cgattccgac tatcagggcc agttgatgat ttccgtgtgg aaccgtggtc aggacagctt
     5161 caccattcaa cctggcgaac gcatcgccca gatgattttt gttccggtag tacaggctga
     5221 atttaatctg gtggaagatt tcgacgccac cgaccgcggt gaaggcggct ttggtcactc
     5281 tggtcgtcag taacacatac gcatccgaat aacgtcataa catagccgca aacatttcgt
     5341 ttgcggtcat agcgtgggtg ccgcctggca agtgcttatt ttcaggggta ttttgtaaca
     5401 tggcagaaaa acaaactgcg aaaaggaacc gtcgcgagga aatacttcag tctctggcgc
     5461 tgatgctgga atccagcgat ggaagccaac gtatcacgac ggcaaaactg gccgcctctg
     5521 tcggcgtttc cgaagcggca ctgtatcgcc acttccccag taagacccgc atgttcgata
     5581 gcctgattga gtttatcgaa gatagcctga ttactcgcat caacctgatt ctgaaagatg
     5641 agaaagacac cacagcgcgc ctgcgtctga ttgtgttgct gcttctcggt tttggtgagc
     5701 gtaatcctgg cctgacccgc atcctcactg gtcatgcgct aatgtttgaa caggatcgcc
     5761 tgcaagggcg catcaaccag ctgttcgagc gtattgaagc gcagctgcgc caggtattgc
     5821 gtgaaaagag aatgcgtgag ggtgaaggtt acaccaccga tgaaaccctg ctggcaagcc
     5881 agatcctggc cttctgtgaa ggtatgctgt cacgttttgt ccgcagcgaa tttaaatacc
     5941 gcccgacgga tgattttgac gcccgctggc cgctaattgc cgcgcagttg cagtaatatg
     6001 acgccggatg acttttcatc cggcgagttt ctttaaacgc caaactcttc gcgataggcc
     6061 ttaaccgccg ccagatgttc cgccatttcc ggcttctctt ccagataggc aatcagatct
     6121 ttcagggtga tgatagagat cactttgcag ttgtaatcac gctcaacttc ctgaatcgcc
     6181 gaaatctcgc cgcgcccgcg ttcctgacga tcgagcgaaa tcaacacgcc agcaagcgtc
     6241 gcgccattgg cctgaataat ctccatcgac tcgcgaatcg ccgttccggc ggtgatcaca
     6301 tcatctacca gcattacgcg tccttgtaac gcgctaccaa ccagattgcc gccttcaccg
     6361 tggtcttttg cttctttgcg gttaaagcag tacggcaggt ccaggtcgtg atgctccgcc
     6421 agtgccacag cggttgtggt ggcaatcggg atccctttgt aagcagggcc aaacagcaga
     6481 tcgaactcaa tgccggaatc caccaacgct tcagcgtaaa aacggcctaa cagtgccaga
     6541 tcgcgcccgg tattaaacag cccggcgttg aagaaatagg ggcttttgcg cccggatttc
     6601 agcgtaaact cgccaaactt taacacctgc ttgctaagcg caaattcaat aaactggcgc
     6661 tgatatgatt tcatgccttc gctcctcatc ttacttttct acagacaaaa aaaaggcgac
     6721 tcatcagtcg ccttaaaaat cagtttgcca gcgccgcctt ctgcgtcgct acaatggatt
     6781 cgattccccc tcgggccagc gccaacaagg tgagtagctc ttcatgggtg aacggctcgc
     6841 cttctgccgt cccctgcact tcaatgatgc gcccgtcttc ggtcatcact acgttcatgt
     6901 cggtctctgc ggctgagtct tcaacgtatt ccagatcgca aattgcttca ccgtttacaa
     6961 taccgacaga aactgcggct accatccctt tcatcgggtt ggttttcagc ttgccgtttt
     7021 ccaccagctt ctgtagcgca tctgccagcg ccacgcaggc acccgtaatc gacgcggtac
     7081 gagtgccacc atcagcctga agcacgtcgc agtccagcgt aatggtgaac tcacccagcg
     7141 ctttcaaatc tactgccgcg cgaagagcac gggcgatcag acgctggatt tccattgtgc
     7201 gtccaccctg cttacctttc gccgcttcac gagcgttacg ggtgtgggta gaacgtggca
     7261 gcatgccgta ctctgcggtg atccagccct ggccctgacc tttcaggaag cgcggcacgc
     7321 cttcttcaat agaggcggta cacaatactt tggtatcgcc aaattcgacc agcaccgagc
     7381 cttctgcatg ttttgtatag ttacgagtca gggtaacggg acgcacctga ttattgctac
     7441 ggcctgctgg acgcatattg aaatctccgg cttgaaacaa atgtggctgc gcattatacg
     7501 gacttccggc ggttattcct atcctgacaa ggcatcgatg gctataatcc ttccacctct
     7561 ccttttataa acaggaacgt ctatgatccg cagtatgacc gcctacgccc ggcgtgaaat
     7621 caagggtgaa tgggggagcg caacctggga aatgcgctcg gtaaaccagc gttatctgga
     7681 aacttacttt cgtctgccgg agcagttccg tagccttgaa cctgtcgttc gcgagcgtat
     7741 tcgttctcgc ctgacgcgcg gtaaagtgga atgtaccctg cgctatgagc cagatgttag
     7801 cgcacaaggt gagctgatcc tcaatgaaaa actggctaaa cagctggtaa ctgccgcgaa
     7861 ctgggtaaaa atgaagagtg acgaagggga aatcaacccg gttgatattc tacgctggcc
     7921 gggcgtgatg gcagcccagg agcaggatct cgacgcgatt gccgctgaaa ttctcgcggc
     7981 gttggatggt acgctggacg actttatcgt cgcgcgcgaa accgaaggtc aggcactgaa
     8041 agcattgatc gagcagcgtc tggaaggcgt caccgccgaa gtggtcaaag tccgcgccca
     8101 tatgccggaa attctgcaat ggcagcgtga gcgtctggtc gcgaagctgg aagatgctca
     8161 ggttcagttg gaaaacaacc gtctggagca ggagttggtt ctgctggcac aacgaattga
     8221 cgttgccgaa gagctggacc gcctcgaagc gcatgttaaa gagacgtaca acattctgaa
     8281 gaaaaaagaa gcggttggtc gtcgtctgga ctttatgatg caggagttca accgtgagtc
     8341 gaacactctt gcgtcgaaat ctatcaatgc cgaagtgaca aactccgcca tcgagctgaa
     8401 agtgttgatt gagcagatgc gcgagcagat tcagaatatt gagtaactcg taacaccaat
     8461 tctgactttt cagccttcac aaatgccgcc cactcaaaca gagcggcatt tttcttcccc
     8521 gcaacttcaa ttctgttttg cgtgcctgct ccagattttg cgatgttttt ttgcccagca
     8581 cactgagaac gtgagatact cacaactgta tataaataca gttacagatt tactttcttt
     8641 gcaatcgata tcacatggag tgggcaatga acgaacatca tcaacctttt gaagagataa
     8701 aactgattaa tgcaaacgga gcagaacaat ggtcagcaag acaacttggg aaactactgg
     8761 gttattcaga gtatcgtcac tttatacctg tattaacgcg cgccaaagag gcctgtgaga
     8821 acagtggtca cacaattgat gaccatttcg aggagatcct cgatatggtc aaaattggct
     8881 caaatgccaa aagagcatta aaagacatcg tactctcccg ctatgcctgt tacctggtag
     8941 tacaaaacgg cgaccccgcg aaaccggtca ttgcggcagg gcagacttat tttactatcc
     9001 agacccgacg gcaggagctt gctgacgatg aagcatttaa gcaacttcgt gaagacgaaa
     9061 agcgtctgtt tctgagaaat gagttgaaag aacataacaa acaattggtt gaggcagcac
     9121 agcaagctgg ggtagcgaca gctactgatt tcgccatatt tcagaatcat ggttaccagg
     9181 ggctgtatgg tggattagat cagaaagcca tccaccaact gaaggggctg aaaaagagtc
     9241 agaagatcct ggatcatatg ggttcaacag aactggcggc taatctcttt cgagctaccc
     9301 aaacagaaga aaaactcaag cgggatagcg tgaattcaaa acagcaagct aataccacac
     9361 actttgacgt gggtagcaag gtgaggcaaa ccattcagga acttggcgga accatacctg
     9421 aggagttgcc gaccccgcag gtcagcatca agcagttgga aaacagtgta aaaattacag
     9481 agaagaaata gcagaaacga aaaactcttt ttgttgattg agacacccga tgcgtaagcc
     9541 aaggtccagg tgcagtgaac acaatggcta aacattgcac ctttcttttc ccccctcagt
     9601 tttaacctat tttttcttat gcattttctc agacaagaag tcagaagaat gcatctacgc
     9661 tacagaaaat agcgatttca cataactaaa agttatcaat ttcctctcac cttaaaaaat
     9721 atcaatcgtg acaatgcgca caaatcgcta ccctgccaga cagattttta gggagagaac
     9781 catgctgtta cacattttgt atttggttgg cattactgcc gaagccatga cgggggcgct
     9841 ggcggccggg cgacggcgca tggatacatt tggcgtaatt attattgcaa ccgccaccgc
     9901 aattggcgga gggtcagtgc gcgatattct gctgggccac tatccgctcg gctgggtcaa
     9961 acacccggaa tatgtgatta tcgtcgctac cgccgcagta ctcaccacta tcgttgctcc
    10021 tgtaatgcct tacctgcgca aagtgtttct ggtgctcgat gcgctcggac tggtggtctt
    10081 ttctatcatc ggcgcacagg ttgcactgga tatggggcac ggcccaatta ttgccgttgt
    10141 cgcggcggta accaccggtg tatttggcgg cgttttgcgc gatatgttct gtaaacgcat
    10201 tccgctggtc tttcaaaaag agttgtatgc cggtgtctca tttgcctccg ccgtgctgta
    10261 catcgcgctg caacactatg tttctaacca tgatgtggtg attatctcca ccctggtatt
    10321 cggctttttt gctcgtttac tggcgctacg tcttaagctg ggattaccgg tcttttacta
    10381 cagccacgaa ggtcactaag gttcaaaacc tgtgatctgc tgggcagcca gccaactgcc
    10441 cagcttcttg atttgcgcat tttccttcca ttcaataacc tgtctggcgc gcccagatcc
    10501 agtccccggc agctgctgcc agaactgctc cgtgctaaat aaaagttgcg accaggaccg
    10561 ttcatcactg gcattaagcg ccgcccgggt tagcggtatt cccattgcca tcacccagcg
    10621 agtaaaaggc tggttacgag ccagattaaa ctgatgccat aactgcgcac ttttactttt
    10681 cgcgatcccc ggcgtgttct gtaattgctc tggcgttaat aaaagccagg aaaagatatg
    10741 ctcaaagcga tgagtctgat gcagcgcgcg ccaaccggcc tcaccaatgc catccagccc
    10801 cagaacctgt tttgacccca gccagactaa gcgtgaaatg aactgttcct gacaaacatc
    10861 agaagcaaag tagcaggtca acgggttaaa gcggttttct ggtggtgtcg gttttgtacg
    10921 ttctgcaccg cgccagacca catcatcaat gcgaggaata ccctgaccgg caaggctgac
    10981 gaggatctga tcaccaggcg caatatccca ctcctgccag cgcctgacgg aaccaatatt
    11041 cacccgctgg acttttttat catccagcat gacaggtgcg agtgacgcaa ccaccgatat
    11101 tttaccgctc ttacccaccg caaactgaat cgccttcact tcggcaacct gagctacagg
    11161 ttgatatttc caggccacca gccactccgc ctggcccggt agccaatgac gggattcggg
    11221 ttccttagcc cctcgcacaa ccacgccatc ggtgacgaag ggtaatttcg ctttccacca
    11281 ctcattgcgt acgcgcgcaa cctcatcagc atttttaacc gcacgggtat acctctgcgt
    11341 tagagtaaaa cctgcggtag ccagctcctt taaacgatcg gtcattaact gcggtccatc
    11401 cggccatgcc cagacaaaaa cagccagtga gtttagcgtg tcgctattcc cctggcgcat
    11461 catcaggcca gcaacttttg cgcgggcatt tattcccccc atttgttgtt ggatatgccc
    11521 ctcgcgctgg agaaatattt ccccctgaag cgtactgttg gctaaaggcc cgctaacggt
    11581 ttgcagcaca gcggaaatta agctaacttt ctgcgtccag tcctcgcctt tcaggccatt
    11641 accgcgactg attgctttgt tcagtttccc gtcccgataa accagggtta ccgcaacgcc
    11701 atcaactttt ggctgcaccc aaagatcgct acgttctcgc atccacagac ttaatgcgtt
    11761 tttatccgcc attttgcgta cacccgtatg ggcaaccgga tgcataaccg caccatttaa
    11821 aggcggcatc atcacgtcgc ggggctcgct cacaaagcag cgttgccact gcgtaagacg
    11881 ggcgcttaac tgatcgtaaa caccgtcttc cacctcactt tttccttcct tccagtagtc
    11941 atcgtcccac tgttttattt gctgttgcag gcgggaaatt tcttcctgtg ctctggctgg
    12001 cgaccaggcc ggacagaccg cccacacaga tgattgccag cacaagatac ttattaatat
    12061 cgccatccat actttcatct tcacctccgc tgtagtcagg ctggcagata tacaacgtgg
    12121 tgaaggcaaa gccgagtggc aaaaacggag tctgcgagga cgcttcctga gaatcgtctt
    12181 tattgcagta aatcacaggc aaatgcggaa acagctacgc aaaacgcaac aactttgcgc
    12241 aaaaagtgtg agcaagggct acgtcacatg gccgcgccgt gtataataag ctcgtatgta
    12301 ggctttattt cgctaatcac atacgaaaga tactcatggc tcaaggcacg ctttatattg
    12361 tttctgcccc cagtggcgcg ggtaaatcca gcctgattca ggctttatta aaaacccaac
    12421 cgttgtatga cacccaggtt tctgtttcac acaccacacg ccaaccgcgt cctggtgaag
    12481 tccacggtga acattatttc tttgttaatc atgatgaatt taaagaaatg attagcagag
    12541 atgcgttcct cgaacacgca gaagtttttg gtaattacta tggcacttcg cgtgaggcca
    12601 ttgagcaagt actggcgacc ggtgtcgatg tttttctcga tatcgactgg cagggcgcgc
    12661 agcaaattcg ccagaagatg ccgcacgcgc ggagtatctt tattttaccg ccgtccaaaa
    12721 ttgaactgga ccgccgtcta cgcggtcgcg gtcaggacag cgaagaggtc attgcaaagc
    12781 gtatggcgca agctgttgca gaaatgagcc attacgccga atatgattat ctgattgtga
    12841 atgatgactt cgataccgcg ttgaccgatt tgaagaccat tattcgcgcc gaacgtctgc
    12901 gcatgagccg ccaaaagcag cgtcatgacg ctttaatcag caaattgttg gcagactgaa
    12961 cctgatttca gtatcatgcc cagtcatttc ttcacctgtg gagcttttta agtatggcac
    13021 gcgtaactgt tcaggacgct gtagagaaaa ttggtaaccg ttttgacctg gtactggtcg
    13081 ccgcgcgtcg cgctcgtcag atgcaggtag gcggaaagga tccgctggta ccggaagaaa
    13141 acgataaaac cactgtaatc gcgctgcgcg aaatcgaaga aggtctgatc aacaaccaga
    13201 tcctcgacgt tcgcgaacgc caggaacagc aagagctgga agccgctgaa ttacaagccg
    13261 ttaccgctat tgctgaaggt cgtcgttaat cacaaagcgg gtcgcccttg tatctgtttg
    13321 aaagcctgaa tcaactgatt caaacctacc tgccggaaga ccaaatcaag cgtctgcggc
    13381 aggcgtatct cgttgcacgt gatgctcacg aggggcaaac acgttcaagc ggtgaaccct
    13441 atatcacgca cccggtagcg gttgcctgca ttctggccga gatgaaactc gactatgaaa
    13501 cgctgatggc ggcgctgctg catgacgtga ttgaagatac tcccgccacc taccaggata
    13561 tggaacagct ttttggtaaa agcgtcgccg agctggtaga gggggtgtcg aaacttgata
    13621 aactcaagtt ccgcgataag aaagaggcgc aggccgaaaa ctttcgcaag atgattatgg
    13681 cgatggtgca ggatatccgc gtcatcctca tcaaacttgc cgaccgtacc cacaacatgc
    13741 gcacgctggg ctcacttcgc ccggacaaac gtcgccgcat cgcccgtgaa actctcgaaa
    13801 tttacagccc gctggcgcac cgtttaggta tccaccacat taaaaccgaa ctcgaagagc
    13861 tgggttttga ggcgctgtat cccaaccgtt atcgcgtaat caaagaagtg gtgaaagccg
    13921 cgcgcggcaa ccgtaaagag atgatccaaa aaatcctctc ggaaatcgaa gggcgtttgc
    13981 aggaagcggg aattccctgc cgcgtcagtg gtcgcgagaa gcatctttat tcgatttact
    14041 gcaaaatggt gctcaaagag cagcgttttc actcaatcat ggacatctac gctttccgcg
    14101 tgatcgtcaa tgattccgac acctgttatc gcgtgctcgg gcaaatgcat agcctgtaca
    14161 agccgcgtcc gggtcgcgtg aaagactaca tcgccattcc aaaagcgaac ggctatcagt
    14221 ctttgcacac ctcgatgatc ggcccgcacg gtgtgccggt tgaggtccag atccgtaccg
    14281 aagatatgga tcagatggcg gagatgggtg ttgccgcgca ctgggcttat aaagagcacg
    14341 gcgaaaccag caccaccgcg caaatccgcg cccagcgctg gatgcaaagc ctgctggagc
    14401 tgcaacagag cgccggtagt tcgtttgaat ttatcgagag cgttaaatcc gatctcttcc
    14461 cggatgagat ttacgttttc acaccggaag ggcgaattgt cgagctgcct gccggtgcaa
    14521 cgcccgtcga cttcgcttat gcagtgcata ccgatatcgg tcatgcctgc gtgggcgcac
    14581 gcgttgaccg ccagccttac ccgctgtcgc agccgcttac cagcggtcaa accgttgaaa
    14641 tcattaccgc accgggcgct cgcccgaatg ccgcgtggct gaactttgtc gttagctcaa
    14701 aagcgcgcgc caaaattcgt cagttgctga aaaacctcaa gcgtgatgat tctgtaagcc
    14761 tgggccgtcg tctgctcaac catgctttgg gtggtagccg taagctgaat gaaatcccgc
    14821 aggaaaatat tcagcgcgag ctggatcgca tgaagctggc aacgcttgac gatctgctgg
    14881 cagaaatcgg acttggtaac gcaatgagcg tggtggtcgc gaaaaatctg caacatgggg
    14941 acgcctccat tccaccggca acccaaagcc acggacatct gcccattaaa ggtgccgatg
    15001 gcgtactgat cacctttgcg aaatgctgcc gccctattcc tggcgacccg attatcgccc
    15061 acgtcagccc cggtaaaggt ctggtgatcc accatgaatc ctgccgtaat atccgtggct
    15121 accagaaaga gccagagaag tttatggctg tggaatggga taaagagacg gcgcaggagt
    15181 tcatcaccga aatcaaggtg gagatgttca atcatcaggg cgcgctggca aacctgacgg
    15241 cggcaattaa caccacgact tcgaatattc aaagtttgaa tacggaagag aaagatggtc
    15301 gcgtctacag cgcctttatt cgtctgaccg ctcgtgaccg tgtgcatctg gcgaatatca
    15361 tgcgcaaaat ccgcgtgatg ccagacgtga ttaaagtcac ccgaaaccga aattaatgtt
    15421 ttatgaaccc aacacgttat gcacgcatct gcgaaatgct cgccaggcgg cagcctgatc
    15481 tgaccgtctg catggagcag gtccacaaac ctcataacgt ttctgcgatt attcgtaccg
    15541 cagatgccgt tggcgtacat gaagttcacg ccgtctggcc tggtagccgc atgcgcacca
    15601 tggcttcggc agcggcgggt agtaacagct gggtacaggt gaaaacacac cgcaccattg
    15661 gcgatgccgt cgctcatctc aaaggccagg gcatgcagat tctggcaacc catctttctg
    15721 ataatgctgt cgatttccgc gaaattgatt acactcgccc gacctgcatt ttgatgggac
    15781 aggagaaaac gggcatcacg caggaagcat tggccctggc ggatcaggac atcatcattc
    15841 cgatgatcgg catggtgcag tcgctgaatg tttccgttgc ctcagccctc attctttacg
    15901 aagcccagcg tcagcggcaa aatgcaggca tgtacctgcg tgaaaacagc atgttgccgg
    15961 aagcagagca acaacgcctg ttgtttgaag gcggctatcc ggtgctggcg aaagtcgcaa
    16021 aacgcaaagg cctgccttat ccccacgtca atcagcaagg cgagatcgaa gctgatgccg
    16081 actggtgggc tactatgcag gctgcagggt aagtgccatg aaaggtcgcc tgttagatgc
    16141 tgtcccactc agttccctaa cgggcgttgg cgcagcactt agtaacaaac tggcgaaaat
    16201 caacctgcat accgtgcagg atctactctt acaccttccc ctgcgctacg aagatcgcac
    16261 ccatctctac cccatcggag aactactgcc gggcgtttat gccacggtgg aaggcgaagt
    16321 gctgaactgc aatatctcct tcggcggtcg gcggatgatg acctgccaga tcagcgacgg
    16381 ttccggcatc ctcaccatgc gctttttcaa tttcagcgcg gcaatgaaaa atagcctggc
    16441 gacgggccgc cgtgtactgg cttatggcga agcaaagcgc ggtaaatatg gtgcggagat
    16501 gatccacccg gaataccgcg tgcagggcga tctcagcacg ccagaattac aggaaacgct
    16561 cacgccggtt tatccaacaa cggaaggcgt aaagcaggcc acgctgcgta aattaaccga
    16621 ccaggcgctg gatctgctcg acacctgcgc cattgaagaa ctcctgccgc cggaactgtc
    16681 acaaggaatg atgacgctac cggaagcgtt gcgcactttg caccgcccgc caccgacgct
    16741 acagcttagc gatctggaaa ccgggcagca tccggcgcaa cgtcgtctga ttctggaaga
    16801 actgctggcg cacaacctca gcatgttagc cttacgtgcc ggagcacagc gttttcatgc
    16861 ccagccgctg agcgccaatg acacgctgaa aaataaactc ctcgccgcct taccgttcaa
    16921 gccaacgggc gcacaggcac gcgtagtggc ggagatcgag catgatatgg cgctggatgt
    16981 gccgatgatg cgcctggtac agggcgatgt aggttccggt aaaacgctgg tcgccgccct
    17041 cgccgcgctg cgtgcgattg cccacggcaa acaggtggca ctgatggcac caaccgaatt
    17101 actcgccgag cagcacgcca ataacttccg caactggttt gcaccgctcg gtatcgaagt
    17161 gggctggctc gccggtaagc agaaaggcaa agcgcgactg tctcagcagg aagccatcgc
    17221 cagcggtcag gtgcagatga ttgtcggtac acacgccatc ttccaggaac aggtgcagtt
    17281 taacggcctg gcgctggtga ttatcgacga acagcatcgt tttggcgtgc atcagcgtct
    17341 ggcattgtgg gagaaaggcc agcagcaggg cttccatccg catcagttga tcatgaccgc
    17401 cacgccgatc ccccgcacgc tggcaatgac ggcgtatgcc gatctcgata cctcggtgat
    17461 agatgagctg ccgccaggcc gcacgccagt gactacggtc gctattcctg atacccgccg
    17521 taccgacatc attgaccgcg tgcgccacgc ctgcataact gaaggtcgtc aggcgtactg
    17581 ggtttgtacg ttgattgaag agtcagagtt actggaagcg caggcggcgg aagctacctg
    17641 ggaagagttg aaactggcgc taccagaatt gaatgttggc ctggtacacg ggcggatgaa
    17701 accagccgag aaacaggcgg tgatggcgtc gtttaaacaa ggtgagctac atctgctggt
    17761 tgccactacc gttattgaag tcggtgttga tgtgcctaac gccagcctga tgattatcga
    17821 aaaccctgag cgtctgggtc tggcgcagtt acaccagctg cgcgggcgcg taggtcgtgg
    17881 cgcggtggct tctcactgcg tgctgctcta caaaacgccg ctttctaaaa cggcgcaaat
    17941 tcgcctgcaa gtgctgcgcg acagtaacga cggttttgtg attgcgcaaa aagatctgga
    18001 gattcgcggc cctggcgaat tgttaggcac gcgtcagacg ggtaatgctg aatttaaagt
    18061 ggcggattta ctgcgcgatc aggcgatgat cccggaagtt cagcgcctgg cgcgacatat
    18121 tcacgaacgt tacccacaac aggcaaaagc cttgatagaa cgctggatgc cggagacaga
    18181 acgttactcg aatgcgtaaa aggcggcggt gagaagaccg ccgcttcatc ggttaaccgg
    18241 caaaaatcgg caacattaaa tacagcttaa tcaccagcgc attgacgata tcgataaaga
    18301 acgcaccgac catcggcacc accaaaaacg ccatgtgcga cgggccaaag cgttcagtga
    18361 tcgcctgcat gttggcgatt gccgttggcg ttgcaccgag gccaaaacca cagtgacccg
    18421 cagccagcac tgccgcatcg tagtttttgc ccatcatgcg ccaggtaacg aagatggcat
    18481 acaacgccat gaagatggtc tgtaccacca gaatcgccag catcggcagc gccagcgaag
    18541 ccagctccca cagtttcagc cccatcaacg ccatcgccag gaacaacgac aagcttacgt
    18601 tacccagcac ggataccgca cgctcaaaga cgcggtaaaa gcccattatt gacagaccgt
    18661 tgctcagaat cacgccaaca aacagtacac agacgaaggt cggcagttca aaagcagtgc
    18721 cagccaaaag ttgcgcaaca attttcccca ccgtcaggca gatagcaatc agcgcgatag
    18781 tttcaatcag caccaacgag gtgatcatgc gtcccacatc cggcttttca aacgccgtcg
    18841 ggacttcctg gtcatccgga ataccgttcg gcgtggtgga gtgtttcacc agatagcgcg
    18901 ccaccggacc gccaatcaag ccgcccagca ccagaccgaa cgttgcacag gccatcgcca
    18961 cttccgtcgc attggtgaag ccataacgtt caatgaacaa tttactccac gcagcgcccg
    19021 taccgtgacc gccggaaagc gtaatagaac cggccaacag ccccatcagc ggatcaagcc
    19081 ctaacaggct agccatacca atgccaatgg cattttgcat caccaacaga ccaacaacca
    19141 caatcaagaa gatgccaacc acacgcccac cggcacgcaa actggcaatg ttggcgttca
    19201 ggccaatggt ggcgaagaaa gccagcatta acggatcgcg cagggacata tcaaagttga
    19261 cttcccagcc catgcttttt ttcagtacta gtagcgccag cgccaccaac aaaccacccg
    19321 caacaggttc cggtatggtg tatttcttca aaaaggagac ggaatggacc aacttacgcc
    19381 cgagcagcaa cgtcagcgtt gcggcaacaa gcgttgctaa agtatcgaga tgaaacatag
    19441 ttactccttt gtatccgcat cactctttca tactcgtcat acttcatcgc gcagtggcaa
    19501 cttcatgaac tatgacgggt atcgagttat gctttcctgg gtcaaattct cagctgttaa
    19561 tgaacaaatg gcatggattt taagcagaaa aataccaaaa gttatataaa aaagtgcatt
    19621 tatctcattt aaatatttag atgaggatgt ggcaaacgtt tgcttttctc tttcggtcag
    19681 ctaaaatgcc cgctttgcta ccacgggatt gttttcgatg tctgtttcca ccctcgagtc
    19741 agaaaatgcg caaccggttg ctcagactca aaacagcgaa ctgatttacc gtcttgaaga
    19801 tcgtccgccg cttcctcaaa ccctgtttgc cgcctgtcag catctgctgg cgatgttcgt
    19861 tgcggtgatc acgccagcgc tattaatctg ccaggcgctg ggtttaccgg cacaagacac
    19921 gcaacacatt attagtatgt cgctgtttgc ctccggtgtg gcatcgatta ttcaaattaa
    19981 ggcctggggt ccggttggct ccgggctgtt gtctattcag ggcaccagct tcaactttgt
    20041 tgccccgctg attatgggcg gtaccgcgct gaaaaccggt ggtgctgatg ttcctaccat
    20101 gatggcggct ttgttcggca cgttgatgct ggcaagttgc accgagatgg tgatctcccg
    20161 cgttctgcat ctggcgcgcc gcattattac gccgctggtt tctggcgttg tggtgatgat
    20221 tatcggcctg tcgctaattc aggtggggct gacctccatt ggcggcggtt acgcagccat
    20281 gagcgataac accttcggcg caccgaaaaa tctgctgctg gcaggcgtgg ttttagcctt
    20341 aattatcctg cttaaccgtc aacgtaaccc ttacttacgc gtggcctcac tggtaattgc
    20401 gatggcggcc ggatatgcgc tggcgtggtt tatgggcatg ttgccagaaa gcaacgaacc
    20461 gatgacgcaa gaactgatta tggtgccaac gccgctctat tacggtcttg gcattgaatg
    20521 gagtctgctg ctgccgctga tgctggtctt tatgatcact tcgctggaaa ccattggcga
    20581 tatcacggcg acctctgacg tttccgaaca gccagtgtcc ggtccgctgt acatgaaacg
    20641 cctgaaaggc ggcgtgctgg caaacggcct gaactcgttt gtttcggcgg tgtttaacac
    20701 cttcccgaac tcctgcttcg ggcaaaacaa cggagtgatc cagttgactg gtgttgccag
    20761 ccgctatgtc ggttttgtcg tcgcgctgat gttgatcgtg ctgggtctgt tcccggcagt
    20821 gagcggtttt gtacaacaca ttccagaacc ggttctgggc ggcgcaacgc ttgtaatgtt
    20881 tggcaccatc gccgcctccg gtgtgcgtat cgtttctcgt gagccgctga accgtcgggc
    20941 gattctgatt atcgcgctgt cgctggcggt tggtctgggc gtgtctcagc agccgctgat
    21001 tttgcagttt gcccctgaat ggctgaaaaa cctgctctcc tccgggatcg ccgcgggcgg
    21061 tattactgcc atcgtgctga atctgatttt cccaccagaa aaacagtaat tcactggcgg
    21121 cggtaagata attaccgccg cagtctcacc tctttcacca ttcccgcctt gaggattgcg
    21181 cgtaaatcgt gcataactcc cttatgtgca tttcacggga tggaagacca tgaaatttat
    21241 tgggaagctg cttctctaca ttctcatcgc tctgttagtg gtgatcgctg gcctctattt
    21301 tcttctgcaa acccgctggg gagcagaaca tatcagcgca tgggtttccg agaatagcga
    21361 ctatcatctg gccttcgggg cgatggatca ccgtttttcc gcgccatctc atatcgtgct
    21421 ggagaatgtc acgtttggtc gtgatggtca gcccgcgccc ctggtggcaa aaagtgtcga
    21481 cattgcgcta agcagtcgcc aactgaccga accacgccat gtcgatacca tcctgctgga
    21541 aaacgggacg ctgaatctca ccgaccagac cgcgccgcta ccgttcaaag ccgatcgtct
    21601 gcaactgcgt gatatggcgt ttaatagccc gaatagcgaa tggaaactga gcgcgcagcg
    21661 ggtaaatggc ggcgtggttc cgtggtcacc agaagccggt aaagtgctgg gtacgaaggc
    21721 gcagattcag tttagtgccg gatcgctttc gctcaatgat gttcctgcca ccaatgtact
    21781 gattgaaggc agtattgata acgatcgcgt tacgctgact aacctgggtg ccgacatcgc
    21841 ccgcgggaca ttaaccggaa acgcgcagcg taacgccgac ggcagctggc aagtggaaaa
    21901 tctgcgcatg gcggatatcc gtctacaaag cgaaaaatcg ctaaccgact tctttgcgcc
    21961 attacgctct gtcccgtcgt tgcagattgg tcgcttggaa gtcatcgatg ctcgtctgca
    22021 aggtccggac tgggcggtga ccgacctcga tctcagcttg cgcaacatga ccttcagtaa
    22081 agatgactgg cagacacagg aaggcaaact gtcgatgaac gccagcgagt tcatttatgg
    22141 ttcgctgcat ttatttgacc cgattataaa cgcggaattt tccccgcagg gcgtagcgct
    22201 gcgccagttc accagccgct gggaaggggg tatggtcaga acgtcaggga actggctgcg
    22261 tgacggtaaa acgttgatcc ttgatgatgc ggcaattgcc gggctggaat ataccttgcc
    22321 gaaaaactgg caacagttgt ggatggaaac gacacccggt tggttaaaca gcctgcaact
    22381 gaagagattt agcgccagcc gcaatctgat cattgatatc gaccctgact tcccgtggca
    22441 gctcaccgcg ctcgatggtt acggtgccaa cctgacgctg gttaccgatc ataaatgggg
    22501 cgtctggagt ggctcggcga atctgaatgc cgccgccgcg acattcaatc gtgttgatgt
    22561 tcgtcgcccg tcgctggcgc tgaccgccaa cagcagcacg gtgaatatca gcgaactgag
    22621 tgcatttact gaaaaaggca ttctggaagc caccgcaagt gtttcacaaa cgccacaacg
    22681 tcagacccat atcagcctga atggacgcgg tgtgccggtg aatattttgc aacaatgggg
    22741 atggcctgaa ttaccgttga ctggcgacgg caatattcag cttaccgcca gtggagatat
    22801 tcaggccaat gccccgctga aacctacggt tagcgggcaa ttgcatgccg tgaacgccgc
    22861 aaagcagcaa gtgactcaaa ccatgaatgc gggcgtcgtt tccagcggtg aagttacatc
    22921 gacggagccg gtgcggtaaa catcaatctc gccgggtacg actcagacgt acccggcatt
    22981 ccgtcaatag attacaacgt aattgtcagc gcattccctt gaggcgtcac caccagcccc
    23041 tgctcacttt cagcctgcga accgccttgc agaccattta ctttcacaac attgcgcaag
    23101 cacagcgtcc agttcttcgc ctcgcccgca ccagtcacag taatcgtgtt gccagtacgt
    23161 gctgctttta aagtaaagat caccgatccg tcagcagcag gcacttcaca gacggcttca
    23221 tgcccgtctt gcagattaaa gaggtggaat gccgtgcctt cgtgccacac gtaatcggga
    23281 cgttgatcgt tgttgcccag cgccagtaga gtgttatcac gcacataaac gggcagactc
    23341 aggaagccgt gctgctgttt atgccagcga ctaccgtcga gttcatcgtt gtgccacagg
    23401 tgtgtccagc gaccttccgg caggtagaac tgcacatcgc ccgcttcagt gaacaccggc
    23461 gcaaccatca cgttgtcgcc taacatgtat tgacggtcaa ggtaatcaca agccggatcg
    23521 tccgggaact ccatcatcat ggcccgcatc atcggcgtac cccgcgcgtt cgcacgcgca
    23581 gcttcacgat acagatacgg catcatgcgg catttcagtt gcgtgaagaa gcgcaccaca
    23641 tcacaggact catcatcgta ggcccacggc acacgataag atttgctacc gtgtaaacgg
    23701 ctatggctgg agagcaaacc aaacgcgcac cagcgtttgt aaacgtgcgc cggagcggta
    23761 ttttcaaagc cgccgatatc gtggctccag aagccaaaac ctgaaaggcc aatagacaaa
    23821 ccaccgcgca ggctttccgc cattgattcg tagttagcgt aacaatcgcc accccagtgt
    23881 accgggaatt tctgcgcacc gacggaggcc gagcgggcaa acaagacagc ttcttcctca
    23941 ccaacggtgt ccttgagcac gttccacacc agttcgttgt agatgtacgc ataatggtta
    24001 tgcattttct gcgaatcgga accgtcaaac cactgaacat caattgggat acgttcgcca
    24061 aagtcggtct taaagcaatc aacgcccatc gcgaccagac ctttcagttt gtcggcgtac
    24121 catttgcagg catccggatt ggtaaagtca taaatcgcca gacctggctg ccatttatcc
    24181 cactgccata gcgaaccgtc cgggcgtttg agtaaatagc ctttctcttg taactcttta
    24241 aagacggggg atttttgacc gatatagggg ttaatccaga cgcagatttt cagccctttc
    24301 gctttcaggc ggcggatcat cccttccggg tcagggaaag tcagcgggtc ccactcaaaa
    24361 tcgcaccact ggaaggcttt catccagaaa cagtcaaagt ggaaaacatg cagcggcaga
    24421 ttgcgttccg ccataccatc gataaagctg tttaccgtcg cttcgtcgta gttggtggta
    24481 aatgaagtgg ttagccacag gccgaaggac cacgggggcg gcagcgccgg acgaccagta
    24541 aagcgggtat aacgatcaag taccgctttc ggcgtcgggc cgtcgataac aaagtattcg
    24601 agatattcac tctcaacgct gaactgcact ttggagactt tctccgatcc cacttcaaaa
    24661 gagacacact ggggatgatt gaccagtacc ccataaccac ggttagtcat gtagaacggg
    24721 atatttttat acgcctgttc agtacttgtg ccgccgtccc ggttccaggt ctctaccgtc
    24781 tggccattgc gcaccagggc agtaaagcgc tctcccagac cgtaaactgt ttcgccaacg
    24841 ccaagatcaa gccgctcaaa catataattg cgttgattat tcgtgtcctg cacgtagcca
    24901 ttatttttca cctgactacc ggtaatacgt tcgccgttgc gcagaaaatc cagtgaccag
    24961 aactcacctt tgctgacacg cgcgcttaag ttgccacttt taaactcagc ataacgttct
    25021 gtgttttcga ttgtgacctt cacgtcctgc aaaatattga gcggataatg aggaccgtta
    25081 ttcagcgccc cctgaaaatg ctcaatccgc acaccgacaa taccttcctg tggggagaaa
    25141 aagcgcagcg taaataaagg cgtatcaagc tgccaggtac gttcacgcac atcacggggg
    25201 gcagcataga ccaccatttc attatcctgc tgttcaacct cgaatacctg aagcgggtga
    25261 atcaaattga ggccaggttg aatcaaccag tttccatcgc taattttcat tgccgttcct
    25321 tagttctgca cttcttgaga ggtgaattgt tgctggcaac ggcgtttacc ctgagccagc
    25381 tgttccataa cggttttcag attgtgcgtc gtgagtgagt agtagcgttt agcgataatc
    25441 gcgctcagca aataacagat cgccggaaca atcgtgaata gcgcaataat gatgctaatc
    25501 gtggcgctgt tctgcgcttt ttctgccgca tcatatccgc cataagccag catccagccg
    25561 ataagagcgc cgccgaaggc caaccccagt ttgagcacaa acagcgtgcc agcaaaactg
    25621 atcccggtca ggcgtttacc attgcaccac tcgccgtagt cgacggtatc gaacatcatt
    25681 acccactgga taggtgtcac cagttgatgc aacacaccaa tcacgaagat gaagacaaac
    25741 atagtgatgc tggcctgcat gggaacaaag aacatcgcga ggctaatcac tgccagcagg
    25801 gcgttcgtcc accagaagat agtgacttta catttccagt cggtcagagg ttttgccagt
    25861 gcggaaccaa tcaggttacc cacgcaataa gtggtgagaa aagcgacaaa cacttccggc
    25921 gtgcccaaaa tccatgtgac gtaatacatc atcgccccac cgcgtacgca caccgccagg
    25981 atattgaaaa tggttagtaa accgacaatc cgccactggt cgttttgcca gatatcacgt
    26041 aaatcttccc gcatagacgt tgttgtaggt ggtgcttcaa cgcgttcttt agtggtgaag
    26101 aaacaaaatg ccagcatcat gaatgccacc acggaaagga ccacgatacc gccctggaaa
    26161 ccgagtggtt tattatcacc gccaattaaa ttaaccagtg gcatcatcag aacagtagaa
    26221 agcatgcctc ccgcggtcgc cagcacaaaa cgccaggatt gcagcgagat acgctgagtc
    26281 gggtcattgg tgattacacc acccaatgcg cagtaaggga tattgacgac ggtataaagt
    26341 aaggtaagtt agggtgtaag taattgctgc atagatcatt ttgccgttca tactgagatc
    26401 tggcgtgcta taggccagta cacagacgat cccgaatggc agtgcgccaa acagtaccca
    26461 cggacgaaat ttaccccagc gagagcgcgt tcgatcggcc aacaacccca tgcaaggatc
    26521 ggaaatcgca tccagtgcac gagcgaccaa aaacatggtt ccgacaaatc cggcaggaat
    26581 gccaaaaata tcggtataaa agaacatcat atataacatt acgttatcga aaataatgtg
    26641 gctggcggcg tctcccatgc cataaccaat tttctcttta acggacaaca cttcactcct
    26701 catctgcttt tccttgatgc tataggggtg ctgagaccga taacgcattt tgtaaaccat
    26761 ccctgtgaga gcgaatattc cgatatctgg ttattaattt atgattcttg ttttatgtga
    26821 tcgtggtagc gttaattccg ctcatatatc attgtaaaat atgggtttta tataaattat
    26881 aatgctttcg tgataatacg ctgcgtttat taggcggaaa aaactgatct gggggatgta
    26941 gaaactcaag gaagtagcta taatgcgccc cgcctccatg tagcaatcga ggcgcggaag
    27001 atcgtcgtct ccggtgaggc ggctggactt caaatccagt tggggccgcc agcggtcccg
    27061 ggcaggttcg actcctgtga tcttccgcca gtttacctct tctgaactct cccaatgtca
    27121 ataaatctct tctaatccct tgtatatcag tatttatttg tatcctgagg tcaacctact
    27181 tcaccccgta tcaagtttct tttgggggta ctttaggggg tgttatttcc tattgaaata
    27241 caagcacccc cataatagga aaaatgtgat acataacaac ggcttttaac aatatctttg
    27301 ttatacaagg ggtgtattgg gggtatcagg ttagttactg gcagaaatcc ccctcaggaa
    27361 catgaattca tcaaaagccg gaggatgtca tggcattaac gatatcaaag tgaaaaccgc
    27421 aaaacccaaa gacaaaccct ataagcttgc tgatggcggc ggtatgtatc tactgattaa
    27481 cactaatggt tctaaatact ggcgcatgaa gtaccgcttt gctggtaaag aaaagatgct
    27541 ttctattggc gtataccccg atgtaacgct agccgatgct cgcgagaaac gcagtgaagc
    27601 caggaaactc ctcgctgctg gaggcgatcc aggggaggca aaaaaggaag aaaaaatcgc
    27661 gcagcaaatg agtctgaaaa atacgttcga ggctattgct cgcgaatggc accagttaaa
    27721 agctgatcgg tggtcattac gctatcgtga tgaaatcatt gatacctttg aaaaagacat
    27781 ttttccctac attggcaagc gccctattgc cgaaatcaaa ccaatgaagt tactggaagc
    27841 actgcgtaaa atggaaaaac gtggagctct ggagaaaatg cgcaaggtcc gccagcgctg
    27901 tggtgaggtg ttccgctatg caattgttac tggccgggca gactataacc cagcccccga
    27961 tcttgccagt gctttagcta caccgaaaaa agtacatttt ccctttctta ctgctaatga
    28021 acttccccac ttcctcaacg atctggcggg ttataccgga agtataatca ctaagacagc
    28081 tactcagatc attatgttaa ccggcgtacg aacacaagaa ctgcgttttg cacgctggga
    28141 agatatcgat tttgagacaa aattgtggga gatcccggca gaagtaatga agatgaagcg
    28201 acctcatatt gtgccgctct ctgagcaggt cattatgcta tttaagcagc ttgaaccaat
    28261 ctcaaaacat catcccctag tctttatcgg tagaaatgat ccccgcaaac caattagtaa
    28321 ggaaagcatt aaccaagtaa ttgaattact tggatataag ggaagactca cagggcacgg
    28381 tttcagacac accatgagta caattctgca tgaacaaggc tttaattccg cctggattga
    28441 aatgcagtta gctcatgtgg ataaaaattc catcaggggt acctataatc atgccctgta
    28501 tctcgatggc cgccgtgaaa tgatgcaatg gtacgctgat tacattgatt cactctccag
    28561 ccgggaaagt taagtcagaa atacttttcc ctgatgtttt cctcaattaa gtattttatc
    28621 ctctggccta caaggctgga ggatttttgt tattatatta tttttttgcg tcttcaaact
    28681 tattacaaaa acataattaa aaacaaattg acatttttaa attggcaatt acaaaataca
    28741 gccttgcagt tatttcgata cctttagcaa aggaatagtt gattcagata taatttagca
    28801 tttcaatcat ctgacatcat tataaaaaat gagattcaca taaagataaa attattgttc
    28861 tcaaggaaag catgaagggg cttcgtaagc aatattcatt cggtgccaag acaggacttg
    28921 atacacagaa tctcaacttt tatgctctca gactatatca ccacatgttt tacaggctca
    28981 gaaaatgaac gatagattat gctttgaagt gcacgataac aagggttatt tcgtatttcc
    29041 ggatacctgg tttggcccat tgctggggga gtttgaagaa gtgcttgatg catacgacgc
    29101 tgatgaaatc agcgagacga gttacatcaa caaactccga cgccttgctc aacgggagcc
    29161 ggactttatt gatatacacg ctcatctggc atatgcattt ctggaacaga atgccccgcg
    29221 taaggcactg aacgccgctc ttaaaggact ggcggcaggt aatcgtatca tcccggaaag
    29281 tttctgtggt gaaataatct ggatgcatcc cgaaaaccgg ccttatctgc gcgcgcttta
    29341 tgcagcaatt ctcgcaaatg tgcacttaca gcgccatcag gatgcggtta tgctcactga
    29401 taaaatactg gcatacaatc ctgaagataa tcagggggct cgctggttgt tgggttcaga
    29461 gctgttacgc accggcgatc atgagcgagc cttcagtgta ctgaaagagc atgctgatga
    29521 attttcacct tactggtatg aactcggtct cctgcatttt ctgaacgggg aacatgttaa
    29581 ggccgccaca gcttttcgcc atggctttgc cacaaacact tacatcgcag aaatgctgtg
    29641 cggaaatctg catccatttc cacttgctgt gtggcatgac ttctccggct ctctggatac
    29701 ggcagaagat tactatgcaa catactcacc attatgggga caatatccgg aagcattgct
    29761 gtttgtaaac tggctctata accattcctc cgtgctgcat gaacgatcag aaatcattaa
    29821 atgcgccgaa atgctgattc aggaagatga tttcgaaata tgcgaaagta ttttaagaca
    29881 gcaggagcac ctgtggaaaa gaattgacaa gacactttct gaggaaattg tacagaaatg
    29941 cagaaatatg aacggtgaat atatctggcc ctggatattg cccttttccg cagcagggat
    30001 gaaacattca agcatccagt atcagtagat attgcattag tgtaacttta aaacaagtaa
    30061 taatttatta tccaataata acactgtgtt ccttccccag gtgattaccc tggggttatt
    30121 ttctgaaagt ttcctttgca aaaggatagc gttgtaaatt actttaacga attactccat
    30181 gaactcatta aagttctttc gtaatacttt atcgtcaaac acgttatagc tggcatcaaa
    30241 aaccacagat ttaccctgcc agcgggtaat cccctcctct ggcgttttcg gttgaactgg
    30301 tatccccgtc caacacaggc agccattata aacagtaaaa aattgtattc gctgttatga
    30361 aatcaatagt cctgacgtac tgttgaatgt gcttgtgttc tgaacctcgt acaaccagta
    30421 catttgatat cgaattataa acaaaacatt gcatccaact tttttaatat ggacaatagt
    30481 tgtccatacg gtggttttat attcgataaa tcaatggatt tcattgagtc acaggactgg
    30541 taatggatga aaatgcttta aggtttgcct catactggcg caactcgctt gcagatgctg
    30601 agtcaggaaa gggctgtttt gaacggaaag acgcccaaaa tttcactcac tggcatggga
    30661 tagcggcggg acgtcttgac gaagcgattg tcaataaatt ttttaaggga gaaaaagacg
    30721 atgtcgaaac tgtcaatgtc atcttacgcc caaaagttta tttccggtta ctgcagcatg
    30781 gtaaggaccg ttccgcaggc acgcctgata ttgttac
//
LOCUS       DAJTYM010000043        29681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTYM010000043 DAJTYM010000000
VERSION     DAJTYM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..200)
                     /gene="wcaD"
                     /locus_tag="OO575_001972"
     CDS             complement(<1..200)
                     /gene="wcaD"
                     /locus_tag="OO575_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6664951.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTA"
     gene            complement(175..1392)
                     /gene="wcaC"
                     /locus_tag="OO575_001973"
     CDS             complement(175..1392)
                     /gene="wcaC"
                     /locus_tag="OO575_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6664952.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1389..1877)
                     /gene="wcaB"
                     /locus_tag="OO575_001974"
     CDS             complement(1389..1877)
                     /gene="wcaB"
                     /locus_tag="OO575_001974"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6664953.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(1880..2719)
                     /gene="wcaA"
                     /locus_tag="OO575_001975"
                     /pseudo
     CDS             complement(1880..2719)
                     /gene="wcaA"
                     /locus_tag="OO575_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(2812..4974)
                     /gene="wzc"
                     /locus_tag="OO575_001976"
     CDS             complement(2812..4974)
                     /gene="wzc"
                     /locus_tag="OO575_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6664954.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(4977..5420)
                     /gene="wzb"
                     /locus_tag="OO575_001977"
     CDS             complement(4977..5420)
                     /gene="wzb"
                     /locus_tag="OO575_001977"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6664955.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5426..6565)
                     /gene="wza"
                     /locus_tag="OO575_001978"
     CDS             complement(5426..6565)
                     /gene="wza"
                     /locus_tag="OO575_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6664956.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            6874..7023
                     /locus_tag="OO575_001979"
     CDS             6874..7023
                     /locus_tag="OO575_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664957.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7224..8807
                     /locus_tag="OO575_001980"
     CDS             7224..8807
                     /locus_tag="OO575_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6664958.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9259..11112)
                     /gene="asmA"
                     /locus_tag="OO575_001981"
     CDS             complement(9259..11112)
                     /gene="asmA"
                     /locus_tag="OO575_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6664959.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11134..11715)
                     /gene="dcd"
                     /locus_tag="OO575_001982"
     CDS             complement(11134..11715)
                     /gene="dcd"
                     /locus_tag="OO575_001982"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6664960.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(11807..12448)
                     /gene="udk"
                     /locus_tag="OO575_001983"
     CDS             complement(11807..12448)
                     /gene="udk"
                     /locus_tag="OO575_001983"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6664961.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            12766..13008
                     /locus_tag="OO575_001984"
                     /pseudo
     CDS             12766..13008
                     /locus_tag="OO575_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13007..13948
                     /gene="mdtA"
                     /locus_tag="OO575_001985"
                     /pseudo
     CDS             13007..13948
                     /gene="mdtA"
                     /locus_tag="OO575_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            13948..17070
                     /gene="mdtB"
                     /locus_tag="OO575_001986"
                     /pseudo
     CDS             13948..17070
                     /gene="mdtB"
                     /locus_tag="OO575_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17071..20136
                     /gene="mdtC"
                     /locus_tag="OO575_001987"
     CDS             17071..20136
                     /gene="mdtC"
                     /locus_tag="OO575_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6664962.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20137..21552
                     /locus_tag="OO575_001988"
     CDS             20137..21552
                     /locus_tag="OO575_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6664963.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            21549..22952
                     /gene="baeS"
                     /locus_tag="OO575_001989"
     CDS             21549..22952
                     /gene="baeS"
                     /locus_tag="OO575_001989"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6664964.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            22949..23671
                     /gene="baeR"
                     /locus_tag="OO575_001990"
     CDS             22949..23671
                     /gene="baeR"
                     /locus_tag="OO575_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6664965.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            23862..24194
                     /locus_tag="OO575_001991"
     CDS             23862..24194
                     /locus_tag="OO575_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6664966.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24341..25702
                     /gene="yegQ"
                     /locus_tag="OO575_001992"
     CDS             24341..25702
                     /gene="yegQ"
                     /locus_tag="OO575_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6664967.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26044..26361)
                     /locus_tag="OO575_001993"
     CDS             complement(26044..26361)
                     /locus_tag="OO575_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664968.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            26767..27666
                     /gene="yegS"
                     /locus_tag="OO575_001994"
     CDS             26767..27666
                     /gene="yegS"
                     /locus_tag="OO575_001994"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6664969.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(27748..28521)
                     /locus_tag="OO575_001995"
     CDS             complement(27748..28521)
                     /locus_tag="OO575_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6664970.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(28627..29667)
                     /gene="gatD"
                     /locus_tag="OO575_001996"
     CDS             complement(28627..29667)
                     /gene="gatD"
                     /locus_tag="OO575_001996"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6664971.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         6806 a         7904 c         7708 g         7263 t
ORIGIN      
        1 gccgtgagtc ccgcgccaat ccctaaggca atcatcaatt ttttaatgct gatgcgttct
       61 aaaaacagca gtaacaagac aggtaaaaaa gtgacgatgg tgatggggaa actttcgcca
      121 agctgggcga ttttgacgtt aaccagcagg tagatcagtg gcagcagcag gtagctacag
      181 attctgatag aagttgacat actcctccag catctgttgt ccactgtagg cggcgcgact
      241 gcgttggctg aactcagcca gcgtggtacc aaatatcgcc tgcgcgattt ccggtttgct
      301 taactgtacc agttgcagca cctcttcttc actgacggtt ttaccaccgg atttttgcag
      361 cacttcccgc gccgcatcgc tatgggtggc aatcaccggc acgccaatcg atagcgcctc
      421 acacaaaatc agcgggtagt tatcgacacg agaactgaac accagcgcat ccatctgatt
      481 gagcgcgctc atcaacttgc gcttgtcagt ttcaaagccg tgattaacca cgttgccagc
      541 ggtgaacggc gagaacttac caaaggtatg cagttcgatt ttgtcgccca gcgccatcat
      601 ctcacgcacc agttgctggt tagttttgcc gtcgtaacgc aggtcatgcg ccaccactgc
      661 gattttcggc ttgccctgag tttcgcgcac cggaggcaga tctgccagaa tcgcttcggt
      721 tgccatatca atgccattat tgataatccg gcaacgccct ggcccgtaca gactattgaa
      781 agcgtcagcc acatgctggc tgggggaaat aaactgacag cccagcgcca gcatctcgcg
      841 gaataactgg cgtttgcccg ccaccagctg gtgcgcgcga tcaatcttca ccggcggata
      901 gttatttaag gtcgggcatt tctggcagcc cgttttccag ccttcgcaac cgtcggtaaa
      961 ggcgcagcgt ccggttacac tccagtggtc gtgcagcgtc cagaccaggg tgacatccgg
     1021 tttatggttt ttcacttttt cgcaaaagcg caccacgctg ttaagattca accagtagct
     1081 gtgcagcaca tgaaaatgca ggaccaccgg acccggtgtg cgagtaatgg tgcgatataa
     1141 ctcattgaaa ttgccgaaca gatcgcgatt aaacagacga aacaacgcaa tgttcgccat
     1201 cgcggtcatc cgcggcgtat gtttgatgac ctgcggatag ttctgatggc tgacgctctc
     1261 tttgccgcct ttgccgtagc cgtagacaaa atgtgacgcc agcccctgtt gcagcgcacg
     1321 ctggtggaga tctaacgcca cacctgctgc cccgccttcc gccagtcgca cattaaattg
     1381 cagaatattc atttaatcac cttcactcgc gctttttctc ccaccaccag cgcgttgtcc
     1441 gggacagagt cgagcaccac gctgcccgca cccacggtga cgttgttacc aagcgtgata
     1501 tcaccaagaa taatgacgtt ggcaccgagt tcgacgccgt tgccaatgtg tggacatgcc
     1561 atgttatcgg caccccgatt gccgatagtg acgccgtggc gaatggtgaa atcatccccc
     1621 gccaccacgt ttttattgat cactacggcg taaccgtgat ggatagtaaa gcggcggcca
     1681 atggtcgcgg cggcctggat ttcataaccg aaaaagcatt cggtgataat gcgatacagc
     1741 accagcagcg gggccgccca cagattgttg aggacgttct ttttgcgcca caccgaacaa
     1801 aaatgagcga cgcgataggc aagtaccatg cagcacgggc gtaaactcca gctgttggcg
     1861 cgcagatctt ccagcatctt tagcgccccc gaataccatc agccagacgt ttgccgttac
     1921 gcaccgacag cagcgtcaac aaggtgcgcc aggtcatacg tttattgcgg atctggtaga
     1981 gggtaaacag ctgatatttt ttactggcgc ggtcgaattt gtctttgtgc ttgcggtaaa
     2041 agtggaagta tccggagaat tttttcggtg acgaggtgat ctgcatttcg ccgtgattga
     2101 tatgcaggat ctgcgtcgcc tcttccactt tccacggttc gccgtactcc accaccatcc
     2161 gcaggaatat gtcgtaatcc tgtgctgctt tcagttcggt atcgaacaaa cactctttga
     2221 aacgccatgc ccaggtaaag acctgattgc caatgatatt acgtttatag aacaggcggc
     2281 gtgagtacgg cgatttggga tacagtggta agctcgccgg ttgggaataa acttctccct
     2341 agcaaacgta gtcgttagcg tacaaaaagg cgtgtgtgac cagttgctgt ttatgggcga
     2401 ggaagacgct caggcggttg ggcgtccatt catcatcatc atcgatcccg gtgatgtatt
     2461 ccccttgcgc cagcataatc gcctggttac gcaccgcgca cgccccgctg ttaatgtcgt
     2521 tgtggatgta agtgatacgc ggatcgttga gggcagtgac gtactgttgc agttgttccc
     2581 aggaggtgga gcaatcatcc acgatgatca tctcccagtt gctgtagtcc tggcgcagga
     2641 ccgattttat cgcccgaatc gccagttgtt ggcggttcca ggttggcata tagattgaga
     2701 tcagcggatt gtctttcata ggttgttctc ccggcttatg agctggtttg cttgccggat
     2761 gcggcgtgag cgccttatcc ggcctacggg gcggtgcgaa tgcaggcgtc gttatttcgc
     2821 atccgactta tattcgtatt cgtaatagcc ataatcctga tacgcgctgg cgcggcggaa
     2881 gatggagttc agaatcaccc ctttcaccgg aataccgttt tgctcaaagc ggctcagact
     2941 ggtttccact tctttcaatg tgttgaccgc ataacgcgcc accattaacg tggttccgac
     3001 atgacgacca acaattgccg catcggtcac tgccagaatc ggcggcgtat caatcaacac
     3061 caggtcatag tttttactcg cccagttcac cagttcggca aagcgttcgc tcattaacag
     3121 ttcagaagga tttggcggca cctgaccgcg cgggatcagg tcaaatttgg caatagaggt
     3181 cggtttagca gcggtagtaa tatcgccctg accaatcaga atctccgaca gcccattgac
     3241 attattggtg cccaacagct cgtgggtgta gcctttgcgc atatcgcagt cgatcaacaa
     3301 cacgcgttta ttggtctggc tgatcaccgc cgccaggttg gcgcagacaa aggttttacc
     3361 gattgacggg ctaaccccgg tcatcatcaa cacattgttc tgcgcctgca tcatcgcgaa
     3421 gtgcagactg gtacgcagac tacggatggc ttcaatcgcc agatcggttg gattccccac
     3481 tgccagtagc tggctctgtt tatagcgttt aacccctttg atggttttga cgctatcgcg
     3541 cgctttctgc cactccgaca gcgggatgct ggcatagacg ctgattccgt gttcttccag
     3601 cacttgcggg ctttcgatgc cgcgattaaa caacgagcgc agcagcacac ccacgataga
     3661 gagcatcagg ccaaggataa tcgcgccgag gataatcagc cctttcttcg gttttagcac
     3721 gccaggctgg gtgattgccg ggtcaataat gcgcacatcg ccgacggtgc tggcttcggt
     3781 gattttcagc tcctgctctt tattcagcag ttgcatatag acctgctgac cagactcgac
     3841 atcgcgggtc agacggacaa tttcctgctg gtttttcggc atcgccgtta cgcgaccgtt
     3901 aagtttggct ttctcttctt ccagcgcctg acgtttctcc agcagcgtgc ggtacgccgg
     3961 gtgaactttg gtgtacagct tggagatttc cgcctcttta aaggtcagtt cgttcaactg
     4021 cgcgtcgatg ttcaccatcg aatcgagcac cgctttcgct tcaagcggca gatcaacaga
     4081 atctttatcc tgacggaagg cattcagttt gttttcggca acatcgaggc ggctacgtac
     4141 ttccggtaac tgttgcgcga ggaaagcgag gcttttcgac gcttccgccg atttgcgctc
     4201 aatattttgt tcctgatagt tacgagcgat gctgttaaga atgtcgcgga tctgttcgcg
     4261 atcttcaccg gtataagtca ggctcagtac gcctgcgtct ttgccgttct ccgttaccgt
     4321 caggctgttt tgcagttggt tgatcatccc caacgtggag tatttggtga cggtaaactc
     4381 actgcccggg cgggcgtgaa tggcttcaac catcagcgtg acgccttctt ttttcagcat
     4441 ctggcccgct tgcccacggg cgctaaagcc gccatcactg ctcagggtgt agtttttgtc
     4501 atccagcaca ttaagcgtaa acacctgatc cgccatctct ttcgggcggt taaaggtagt
     4561 cactttcacc gtctcgttct gacgccccat caggcgatcc cagcccgccc cgaaaatcgg
     4621 gaacgtgttt ttgctcaccg caatatcgag gtcgagatcg tccaccgttt taccaagcac
     4681 cagacgcgaa cgaatcaact ggatctcggc gtccgatgca ggcggtttgt tggctaatgc
     4741 cgagccgata tcctgcacca gcgaattgcc gctgttttgc tcgatttgta ccagtgcgtc
     4801 ggcactataa atcggcgtgg cgaagaaagt gtaaaccacg gcacagaggg caaatacgac
     4861 ggtgatgcca atcacccacc agcgcgcttc aatgacggtg ccgaccaggc gaccaatatc
     4921 gatttcatca ctgcccgtta ccggagcggc atgttgtttt actttttctg tcattcttat
     4981 acctgctctg cgttcaatgc ctgcgcccac tggcgggcag accgttcaag taatgtgtac
     5041 accgctgcaa acgtttcccg gcttttgtga tacggatcgg ggatttcaca ttcgttatcc
     5101 cagtgaccaa acagcatcac tttgccgcgc atctccggtg ccatctcgca taagcgttcg
     5161 atatggcgct tatccatggt caaaatcagg tcatagttgc gacacaggcg gcggctgatt
     5221 tgtcgtgcac agtggccttc cagagacagt tgatgttctg cggcgacgct gatagcggta
     5281 ggatcagcgc ccttaccgac cagcgcgccg agtccagcgg actccacttt cagttccggg
     5341 tgataacgtt gcagtaagcg ttccgccatc ggggaacggc aaatattgcc gacacagaca
     5401 actaagatgt tgttaaacat gacgattacc agttatgaat gtcgctggct gtatccgtca
     5461 tgtaacggac accgctaata gttggcagca actgattgat cagacggttc cagcgggaaa
     5521 ccggggcggt ggtgacatac accacgtcat aaggttgcag gcggaattct gtcgccatca
     5581 ccagcgacgt cgcatcggac atatccagct ggtagatatt ggcaatcttg ccgttacgcc
     5641 cgccctcgcc tttcagcgga cgaatgacaa agatgccgct ggcgttggag gtggtcatgt
     5701 cgataccttc ggcattggcc agagcttcag tcagggtcat gccgctaaag tccattttca
     5761 gggtgctctg tttcttcact tcacccatca caaatacttt cagatcatca ttacgcggca
     5821 cgtagagaat atcgccggga taaagcagtc ggttctgatt gaggtcgccg ttttgcatca
     5881 gcgcctgtaa agaaatgcgt tcttcacgac cattgtgtgt tagcaccacg ttgcgccagt
     5941 cagcggtgtc ggtcaggcca cctgcggcgt tgatggcgtc gagaatggtc agtggtacgt
     6001 tggtgatcgc ctgttgaccg gatttattca cctgacctga gatataggcc ttttgtgagc
     6061 ggaaggcggc gatattaacg tccacctgcg ggtcagcgat gtacgtcgct aagcgcccgg
     6121 taatatcact gcggatttca gcgagcgttt tcccgactac gtgaaccttg ccgatatacg
     6181 ggtaaaacat agtgccgtca ggctgtaccc agttgccggt gtcgctggag ctgcggtact
     6241 gaccagctgg cgtggtgagt tccgggtgat cccagacggt gacattaaga acgtcccccg
     6301 gcccgacgcg atactggtaa ttcgcgatct cgctttccag cgtcatattg gggcgcgcta
     6361 cattcgggcg tgggcgtaat tggtcaatca ggcgcggggt cagcggataa acattcacca
     6421 ttttgtcgag atcgaaatca gcgtcctgct gtttgatgac gtctttgccc atcgtcgaca
     6481 tattgctgcc cggaagtact gtgcaaccgc ttatcaaggt tactgacacc aataatggca
     6541 tcaatttcat tttggatttc atcattgttt atttatcact ttggcagagt aattatcctg
     6601 tgcactatta atagcaatgt cgccatgcac atttaccttg cagttaattg aataaaaatt
     6661 taactggcat cagtcctaaa aaaattgatt tcatccgcag gctattgaca gaataattca
     6721 gactggtctt tcaggcatcc agacacgcta ccgcccctgg ctttttagct accaatacac
     6781 tgattgagct taatttttca aaccctctct gcatacaatc gttgatgaga aagggttatt
     6841 gcggaaatta acttccgaat ataaggtgac attatggtaa ttgaatattg gctcgccaat
     6901 aatgcaggag gaagtgttac agctaacgga atagcaggca aggtaacaat tcggcaattg
     6961 gctattttta agaattatat taagtatcat tcaatatggt ttttaggagt ttctttaggt
     7021 tgacaatatt taatattgcg tctccacatg cgatatttct taaataatgt tttattatta
     7081 ccgcttttaa ttcagggata atgtgaggtt attaccctca aataatatga gataaatagt
     7141 gctgcaacat tgcatttttg ccccgattta tccatgatcg aattgtgaca tttgtcatac
     7201 aacgaatagg ttttgtactt actatggaat ggattgccga tccgtctatc tgggccgggt
     7261 taatcacgct gattgtgatc gaactggtcc tcggcattga taacctggtc tttattgcca
     7321 tcctcgccga aaaactaccg ccgaagcagc gcgaccgcgc acgggttacc gggctgctgc
     7381 tggcgatgtt aatgcgcctg ttactgctag cgtcaatctc ctggctggtc accctgactc
     7441 aaccgctgtt cagcttccgc tcgtttacct ttagcgcccg cgacttaatc atgctgtttg
     7501 gtggtttctt cctgttgttc aaagccacga tggagctgaa cgaacggctg gaagggaaag
     7561 acagcaataa tcccacacaa cgcaaaggtg cgaagttctg gggcgtggtg acgcaaattg
     7621 tggtactgga cgccattttc tcactcgact cagtgattac cgccgtcggg atggtcgacc
     7681 atttactggt catgatggcc gccgtggtta tcgctatcag cctgatgttg atggccagca
     7741 agccgttaac gcaatttgtt aacagtcacc cgaccatcgt tattctctgc ttaagcttcc
     7801 tgttgatgat tggctttagc ctggtggcag aaggtttcgg cttcgtcatt ccgaaaggct
     7861 acctgtacgc tgccattggt ttctcggtga tgatcgaggc actcaatcag ttggctatct
     7921 ttaaccggcg acgttttctt tccgctaatc agacgctacg ccagcggact accgaagcgg
     7981 taatgcgcct gcttagcggg caaaaagaag atgcggaact ggacgccaaa accgcgtcca
     8041 tgctggtgga tcatggaaac cagcagatct ttaatccgca ggaacggcgg atgattgagc
     8101 gggtacttaa tcttaaccag cgtaccgtaa gcagcattat gacgtcgcgc cacgatattg
     8161 agcatatcga tctcaacgcg ccggaagaag aaatccgcca gttgctggag cgaaatcagc
     8221 atacgcggct ggtggttacc gacggtgatg acgcagaaga tttgctcggt gttgttcacg
     8281 ttatcgacct gctacaacag tcactgcgcg gcgaaccgct caacctgcgg gtgttgattc
     8341 gccagccgct ggtgttcccg gaaaccttgc cgttgttacc agccctggag cagttccgta
     8401 atgcccgcac gcactttgct tttgtggtgg atgagtttgg ctcggtggaa gggattgtga
     8461 cattaagtga cgtcactgaa accattgccg gtaacttacc gaacgaagtg gaagagatcg
     8521 acgcccgcca tgatattcag aagaatgcgg acggttcctg gacggcgaat ggtcatatgc
     8581 cgctggagga tctggtgcaa tatgtgccgc tgccgctgga tgaaaaacgt gaatatcaca
     8641 ccattgccgg gctgttgatg gagtatttgc agcgtattcc aaaacctggc gaagaagttc
     8701 aggtggggga ttatttgctt aaaacgttgc aggtggaaag ccatcgcgtg cagaaggtgc
     8761 agattatacc gctgcgtaag gatggcgaga tggagtacga ggtgtgatgg tatcggccat
     8821 tttacccaat gctgcgtaac gccttataac ttcagcaatg aagcgttgtc tgtggtaaac
     8881 ggacatcaca gcgggggtgt gggtccggct gtaaatcgcc gaatcgtttc ctgtaggctg
     8941 gggcagccca catggatgtg ggctgagggc gcgttttaca gggatgttac ctcgcgcccg
     9001 tacccgatag ccgaaaggaa taagatgagg gaaccgcgcc agcggcgatt taccgccggg
     9061 agcccgggtt gccagggtgg tggcgattga gccaccctgg cacgttcacc ggcttgagcg
     9121 ttccatagta gcgaggaaca tcaagtgaac ggaacagcct tcgatcttac atattcagaa
     9181 tataatagtg ccgccgcgaa cgcctgatgc gacgctttac gcgtcttatc aggcctacag
     9241 gacaggtcac ctggtaacct acatcttctc cagcaacttc ttcacatctt tgccattgcg
     9301 ggaatcttta ttccgctcgg cccagtcatt caggcgacgt ttcgcttcgt cctgtagatg
     9361 tttgcgcagt aactgatcca cttgcaggct gtaattgagt tgctgccagt tgccataaac
     9421 ccgcagcggt actggcgttt ctttgaggaa atcaatcagt ttgctttccc cgttccagcc
     9481 acccacgacc cgaatatcaa actgggtgtc gcaggtttga tctgccagat tcaacatgcc
     9541 ttcccctgtc agcgccagca caggcgattg accttgcatg tcgtttaacg tcacgacgcc
     9601 atccttcaac gtcaaatcgg tggtaaagcg gtcaagccgc gtcacgttat cgaagttttc
     9661 agcggccttc acatcaccgc cattacgctc taccgcttgc tgaatcatct gctggaagtt
     9721 catcccttcc atacgtgtgt cggtcatctc aacatgcgcc tgtccttgcc agttgtggcg
     9781 gaatgcgtcg gcatctatgt cagcaccgga gaaatcacca gccagtgaca tttttccggt
     9841 caacgaaatc ggatagttaa acgccttcag aatggtacca atctcaacgt tctccagccg
     9901 tggctggaag tttatccgcg gatttattga tgtcgcgtcc agcgtgcccg gcagtgaaac
     9961 ctttccaccg ttaagcttgc cctgcagttg agtaatttcc agcaaacccg acttgttggt
    10021 catttgcgtg gcaacatctg taaaattcat tccgcgccag cgcacgttac tggcctgcaa
    10081 caaaatatca gctgtaaagc cttgcagtcc ctgataggcc ggttcatcaa tacgcgaaga
    10141 aatgaccggg cgcggcaacg ttgattggct ctgcccctgc tgcgcggcac cgttttcacc
    10201 attcgcggtt tcattaagcg ggatgaggtt gtcaagattc agttgcggga attgcagtct
    10261 cagctgccat tccggtttct ctgtcagcgt gacctgtgct tgtccgctca gcgtactgtc
    10321 attggcggtc aaactaattt ggttaaatga aaggcgttta tgtgactcct gccactggga
    10381 ctggaaactc ccctgcccct gaataccttg ttttggtaaa tcggcaccct gcaactgcca
    10441 gttaatttgt tcaatagccg ctgttaaatc atgcggataa tcagaagcat ctaccgtacc
    10501 gttaagggat atcgtgagat cgcgctgatc gcgattaacc cgcccggaga actcaaatga
    10561 gccacgatgt tggggatctt gttccatttg caggcggata ttgcggattg tcacctgctc
    10621 gtcatcttca tgctggaaca ccagaacgct gtccgccacc ttaagactgg atatatcaaa
    10681 cgaccatccg cgatcgtctg acagatccgg caaggtattg tcgcgcggtg caaccggagc
    10741 gtcttcactg cgcaccgctt ccgtctgcgg cgtcagttgg atcactgccc cttttagcat
    10801 cacctgctta acgctcagtt gatgactcag tagtggtaaa agcgccacgt ccagacgcat
    10861 gttgtcggcg cgaaccagtg gctggcttgc gccctgggcg gtgagagaca ttcgcccgga
    10921 gaggatacta agctgcggcc agacgtgcca acgcagtggc ccgtcgagct gcaattgata
    10981 accgctacgt gcagcaactt gcttgaccat atagtcgcgg aaatcattcg gattcaccag
    11041 caacactaac gcagataacc cggccaccag cacgaccaag agtatcatca gcgtcgtcag
    11101 aaatcgtctc atggtatcct caatgggcct gaattagtct ttatcgattc ggctggctac
    11161 cgcgccctgc tggttgcgat atttcgcatc ttcacggcgg ttgtaaggtc gcgccgccgg
    11221 gccggaaagc ggctcaaagc tcagcgcacc aattaacatg cccggacgca gcgccagcgg
    11281 cagcttaccg gagttgtaga actccagcac aatgcaacca gaccagcccg gatcgatacg
    11341 gtgcgcggtg acgtgcacca tcagccccag acgcgccaaa gaggaacgcc cgtccagcca
    11401 gcccaccaga tcggctggca gcgtcaccga ctccagtgtc accgccagcg ccagctctcc
    11461 tgggtgaaga tagaacgcct cgccctcgtc gagaacgatc tcatcgctca tcacgcggtc
    11521 aagcgcggcg ctcacttcat ctttgggacc gctcagatcg ataaacgctg ccgtgtgacc
    11581 acggaaggta cgaaatttat tgcccaggcg tacatccacc gtcgcgccgt taatacgctc
    11641 cactggcgga cgtgggttga tcgacaaacg gccttcatca agccaggctt caatatctcg
    11701 gtcacacaga cgcatggcat tttctccttt cgcacatcac tcccttaacg ccaattacgt
    11761 caagggcata ccaacttatc actgaacggt acacaattta tcaagcttat tcaaagaact
    11821 gacttatttt cgctttcaat atatcgatcg cgatgcggtt tttcccgccg cgcggcacga
    11881 taatgtccgc atattgttta gaaggctcaa tgaattgcag gaacatcggg cgcacggttt
    11941 tttgatattg cgccatcacg gaatccattg aacgcccacg ctcgttaacg tcacgcttga
    12001 tgcggcgcat caggcagata tccagcgggg tatcaacgaa aatggagaag ttaagttcgt
    12061 cacgcaaacg cgcatccgtc agcaacaaaa tgccttcgag aatgatgacc ttcttcggct
    12121 caaccgtcac cgtttctttc atacgcgtat gttcaacata gctgtaaacc ggcaggtcaa
    12181 ttgccgagcc gcgtttcaac gcttgtaaat gctcaagcaa caggctgtga tccatcgcgc
    12241 tgggatggtc gtagttggtc ttaacgcgtt cttccatcga cagatggctt tgatctttgt
    12301 aatagcagtc ttcgggaatt acgccgatgt gttcatcacc gacttgctca cgtaattcac
    12361 gataaagggt actggcaata agactcttgc cggaagccga tgcgccagcg ataccgataa
    12421 tgacgcactg atgagactga tcagtcatat atttagcgac ctgattaacc tggatgttag
    12481 gaagggggcg acgaagcgcc aaacgcggca attataggga tttcatccgt ctgataccag
    12541 tcgaatagcg ttgccgcgcg ctcagagtta attgttgaca aagaattccc ggtggcaaat
    12601 tacgttgatc agttttatgt aaggtaaaaa atgttatacg cagttgcgca aattatccgc
    12661 ctttacgtca ctttatgagc aattcgcata taaaatgtaa aacttttgta ctagcataaa
    12721 cacagaaacg aatactggcg acctggcctt gcggataaag cggtaataag caaacaatca
    12781 cagcatgtat taattgccct gccccacccg ctgcttcacc tggtcagttt aggtttagtc
    12841 tcgtttatct ttacccttgt ctcgcttgag ctttcgcagt ttggcaccca actcgcccca
    12901 ctgtggttcc cgacgtccat catgatggtg gcgttttatc gccatgccgg gcgcatgtgg
    12961 ccgggaattg cgctgagctg ttcgctggga aatatcgccg catccattcc aggaaggcca
    13021 gcaggtcaaa gcaggcgatt tactggcaga aattgacccc agccagttca aagttgcatt
    13081 agcacaaacc cagggccaac tggcaaaaga taaagccacg cttgctaacg cccgccgtga
    13141 tctggcgcgt tatcagcaac tggcaaaaac caatctcgtc tcccgtcagg agctggatgc
    13201 ccaacaggcg ctggtcagtg aaaccgaagg caccattaag gctgatgaag caagcgtcgc
    13261 cagcgcgcag ttgcaactcg actggagccg catcaccgca ccagtcgatg gtcgcgttgg
    13321 tctcaagcaa gttgatgttg gtaaccaaat ctccagtggt gataccaccg gaattgtggt
    13381 gatcacccag acgcatccta tcgatttagt ctttaccctg ccggaaagcg atatcgctac
    13441 tgtagtgcag gcgcaaaaag ccggaaaacc gctgatggta gaagcctggg atcgcaccaa
    13501 ttcgaagaaa ttaagtgaag gcacgctgtt aagcctcgat aaccaaatcg atgccacaac
    13561 cggtacgatt aaagtgaaag cacgctttaa taatcaggat gatgcgctgt ttcccaatca
    13621 gtttgttaac gcgcgcatgt tagtcgacac cgaacaaaac gccgtagtga tcccaacagc
    13681 cgccctgcaa atgggcaatg aaggccattt tgtctgggtg ctgaatagcg aaaacaaggt
    13741 cagcaaacat ctggtgacgc cgggcattca ggacagtcag aaagtggtga tccgtgcagg
    13801 tatttctgcg ggcgatcgcg tggtgacaga cggcattgat cgcctgaccg aaggggcgaa
    13861 agtggaagtg gtggaagccc agagcgccac cactccggaa gagaaagcca ccagccgcga
    13921 atacgcgaaa aaaggagcac gctcctgatg caggtgttac ccccgagcag cacaggcggc
    13981 ccgtcgcgcc tgtttattat gcgtcctgtc gccaccacgc tgctgatggt ggcgatctta
    14041 ctcgccggga ttatcggtta tcgcgccctg cccgtttcgg cgctgccgga agtggactat
    14101 ccgaccattc aggtggtcac gctctaccca ggtgccagcc cggatgtcat gacctctgcc
    14161 gttaccgcgc cgctcgaacg ccagttcggg cagatgtctg gcctgaaaca gatgtcgtcg
    14221 caaagttccg gcggtgcgtc agttatcact ttgcagttcc agctaacatt accgctcaat
    14281 gtcgccgagc aggaagtgca ggccgcgatt aacgctgcga ccaacttgtt gccgagcgat
    14341 ctgcctaacc cgccggttta cagcaaagtg aacccggcag atccgccgat catgacgctc
    14401 gccgtcacct caaccgccat gccgatgacg caagtggaag atatggtgga aacccgcgtc
    14461 gcgcagaaaa tctcgcagat ttccggcgtc ggcctggtga cgctttccgg cggtcagcgt
    14521 ccggctgttc gcgtcaaact taacgctcag gcgattgccg ccctcggcct gaccagcgaa
    14581 accgtgcgca ccgccattac cggcgctaac gttaactcgg caaaaggtag cctcgacggc
    14641 ccttcccgtg cggtcacgct ttccgcgaac gaccagatgc aatccgccga agagtatcgc
    14701 cagctaatca tcgcctacca gaacggcgcg ccaattcgtc tgggcgatgt cgcaactgta
    14761 gagcaaggtg cagaaaacag ctggctcggc gcgtgggcga acaaagaaca ggccattgtg
    14821 atgaatgttc agcgccagcc cggtgctaac attatctcca ccgccgacag cattcggcag
    14881 atgctgccac agctcactga gagtctgccg aaatcggtga aggtgacagt gctttccgat
    14941 cgcaccacca atatccgcgc atccgtcgat gatactcagt ttgaattgat gatggctatc
    15001 gcgctggtag tcatgattat ctacctgttt ttgcgcaata ttccggcgac catcattccc
    15061 ggtgttgctg taccgctgtc gttaatcggc actttcgcgg ttatggtgtt tctcgatttt
    15121 tcaatcaata acctgacact gatggcttta actatcgcca ccggattcgt ggtcgatgac
    15181 gccatcgtgg tgatcgaaaa catttcccgc tatatcgaaa aaggcgaaaa accgttggcg
    15241 gcggcgctca agggcgcagg tgaaatcggc tttaccatta tctcgctgac cttctcactg
    15301 attgcggtgt tgatcccact gctgtttatg ggcgatatcg tcgggcgact gttccgcgaa
    15361 tttgctatta ccctggcggt agcgattttg atctcagcgg tggtgtcgct gaccctgaca
    15421 ccgatgatgt gcgcgcggat gctcagccag gagtcgttgc gtaaacagaa cagcttctcc
    15481 cgtgcctcgg aaaaaatgtt cgacaggata atcgccgcct atggtcgtgg actggcgaaa
    15541 gtgctgaatc atccgtggct gaccttaagc gtggcactca gcacgctgct gcttagcgtg
    15601 ctgctgtggg tgttcattcc gaaaggtttc ttcccggtac aggacaacgg cattattcag
    15661 ggcactttgc aggcaccgca atccagctcc tttgccaata tggcccagcg acaacgccag
    15721 gtcgcggacg tgattttgca ggatccggca gtgcaaagcc tgacctcatt tgttggcgtt
    15781 gatggcacta acccgtcgct gaatagtgca cgtttacaaa tcaacctcaa accgttggat
    15841 gaacgtgatg atcgggtgca aaaagtcatc gcccgtctgc aaacggcggt agataaagtg
    15901 ccgggcgtcg atctcttcct gcaaccaacg caggacctga ccatcgatac tcaggtcagc
    15961 cgcacccagt accagtttac cttgcaggcc acgtcactgg atgcgctcag tacctgggtg
    16021 ccacagttga tggaaaaact ccagcaactg ccacagcttt ctgatgtctc cagcgactgg
    16081 caggacaaag ggctggtggc gtatgtcaat gttgatcgcg acagcgccag ccgtctgggg
    16141 atcagcatgg cggacgtcga taacgccctg tacaacgcgt ttggtcagcg actgatttcc
    16201 actatttata ctcaggctaa ccagtatcgc gtggtgctgg agcacaatac cgaaaatacc
    16261 ccaggcctcg cggcgctgga taccattcgc ctgaccagca gcgacggtgg cgtggtgccg
    16321 ttaagctcaa ttgccaaaat tgagcagcgt tttgcgccgc tttccatcaa ccatctggat
    16381 cagttcccgg taacaaccat ctcctttaac gtgccggata actattcgct gggcgatgcg
    16441 gtgcaggcga ttatggacac cgaaaagacg ctgaatctgc cggtggatat caccacgcaa
    16501 ttctagggca gcaccctcgc cttccagtcg gcgctgggca gcactgtctg gctgattgtc
    16561 gcggcggtgg tggcgatgta tatcgtgctc ggcattctgt acgagagctt tattcacccg
    16621 atcaccattc tctcgacgct acccaccgca ggggtcggcg cgctgctggc attaatgatt
    16681 gccggtagcg aactggatgt gattgcgatt atcggcatta ttttgctgat cggtattgtg
    16741 aagaagaacg ccatcatgat gatcgacttc gcgctggctg ctgagcgcga gcaaggcatg
    16801 tcgccgcgcg atgcaatcta ccaggcttgt ctgttgcgtt ttcgtccgat cctgatgacc
    16861 accctggcgg ctctgcttgg cgcgctgccg ctgatgttga gtaccggggt cggcgcggaa
    16921 ctgcgtcgtc cgttaggtat cggcatggtc ggcggtctga ttgtcagcca ggtgctgacg
    16981 ctgttcacca cgccggtgat ttatctgctg ttcgaccgcc tggcattgtg gaccaaaagt
    17041 cgctttgctc gccatgaaga ggaggcgtaa gtgaagtttt ttgccctctt catttaccgc
    17101 ccggtggcga cgattttact gtcggtcgcc attaccctgt gcggcatact gggtttccgt
    17161 atgctgccgg tcgccccgct gccgcaggtc gattttccgg tgattatggt cagcgcctcg
    17221 ctgcccggtg cgtcgccgga gacaatggcg tcttccgttg ccacgccgct ggagcgctca
    17281 cttgggcgca ttgccggggt tagtgaaatg acctccagca gttcgctcgg cagcacgcgc
    17341 attattttgc agtttgattt tgaccgggat atcaacggcg cagcgcgtga tgtgcaggcg
    17401 gcgatcaacg ctgcacaaag tttgctgccc agcgggatgc ccagccgccc gacctatcgc
    17461 aaagcgaacc cgtcggatgc gccaattatg atcctcacgc tgacatccga tacttattcg
    17521 cagggtgaac tgtacgattt cgcctcgacg cagctggctc cgacgatttc gcaaatcgac
    17581 ggtgtcggtg atgtcgatgt cggtggcagc tcgctgcccg ccgtgcgcgt cgggctgacc
    17641 ccgcaggcgc tgtttaatca gggcgtgtcg ctggacgacg tacgcactgc catcagcaat
    17701 gccaacgtgc gtaaaccgca gggggcgctg gaagatggca ctcaccgctg gcagatccag
    17761 accaacgatg aactgaaaac cgccgctgaa tatcagccgc tgattattca ctacaacaac
    17821 ggcggcgcgg ttcgtctggg cgatgtggcg acggtgactg actcagtgca ggatgtgcgc
    17881 aacgccggga tgaccaacgc caaaccggct attttactga tgatccgcaa actgccggaa
    17941 gccaatatta tccagacggt agacagcatc cgggcaaaat taccagagtt gcaggagacc
    18001 attccggcgg cgattgatct gcaaattgcc caggatcgct cccccaccat tcgcgcctcg
    18061 ctggaagaag tcgagcaaac gctgattatc tcggtggcgc tggtgattct ggtggtcttt
    18121 ttattcctgc gctcgggtcg cgccactatt attcccgccg ttgcggtgcc ggtttcgctg
    18181 attggtacgt ttgcggcgat gtacctgtgc ggattcagtc tcaataatct ttcgctgatg
    18241 gcgctcacca ttgctactgg tttcgtggtg gatgacgcca ttgtggtgct ggaaaacatt
    18301 gcccgtcatc tggaagcggg gatgaaacca ctacaggccg cgctgcaagg tactcgcgaa
    18361 gtcggcttta cggtgctgtc gatgagtctg tcactggtgg cggtgttcct gccgctgctg
    18421 ttgatgggcg gattgccggg ccgactgtta cgcgagtttg ccgtgacgct ttctgtcgcc
    18481 attggtattt cgttgctggt ttccctgacg ctaacgccaa tgatgtgcgg ctggatgcta
    18541 aaagccagca agccgcgtga gcaaaagcga ctgcgtggtt ttggccgcat gttggtagcc
    18601 ctgcaacaag gctacggcaa gtcgctgaaa tgggtgctca atcatacccg tctggtgggc
    18661 gtggtgctgc ttggcaccat tgcgctgaat atctcgatcc cgaaaacctt cttcccggag
    18721 caggacaccg gcgtactgat gggtgggata caggcggatc agagtatttc gtttcaggcg
    18781 atgcgcggca aattgcagga tttcatgaaa attatccgtg acgatccggc agtggataat
    18841 gtcaccggct ttaccggcgg ttcacgagtt aacagtggga tgatgtttat caccctcaag
    18901 ccacgcgacg aacgcagcga aacggcgcag caaattatcg accgtctgcg cgtgaagctg
    18961 gcgaaagaac cgggggcgaa tctgttcctg atggcggtac aagatattcg cgttggtggg
    19021 cgtcagtcga acgccagcta ccagtacacg ttgttatccg acgacctggc ggcactgcgc
    19081 gaatgggagc cgaaaatccg caaaaaactg gcgacgttgc cggaactggc ggacgtgaac
    19141 tccgatcagc aggataacgg cgcggagatg aatctggttt acgaccgcga caccatggca
    19201 cggctgggaa tcgacgtaca ggccgccaac agtctgttaa ataacgcctt cggtcagcgg
    19261 caaatctcga ccatttacca gccgatgaac cagtataaag tggtgatgga agtggatccg
    19321 cgctataccc aggacatcag tgcgctggaa aaaatgttcg ttatcaataa cgaaggcaaa
    19381 gcgatcccgc tgtcgtattt cgctaaatgg caaccggcga atgccccact atcggtgaat
    19441 catcagggat tatcggcggc ctcgaccatc tcgttcaacc tgccgaccgg aaaatcgctc
    19501 tcggacgcca gtgcggcgat cgatcgcgca atgacccagc ttggtgtgcc ttcgacggtg
    19561 cgcggcagtt ttgccggcac ggcgcaggtt ttccaggaga cgatgaactc gcaggtgatc
    19621 ctaattatcg ccgccatcgc cacggtgtat atcgtgctgg gtatccttta cgagagttac
    19681 gtacatccgc tgacgattct ctccaccctg ccctcggcgg gcgttggagc gctgttggcg
    19741 ctggagctgt tcaatgcccc gttcagccta atcgccctga tagggatcat gctattaatc
    19801 ggcatcgtga agaaaaacgc cattatgatg gtcgattttg cgcttgaagc ccaacggcac
    19861 ggtaacctga cgccgcagga agctattttc caggcctgtc tgctgcgttt tcgcccgatt
    19921 atgatgacca ccctggcggc gctgtttggc gcgctgccgc tggtattgtc gggcggtgac
    19981 ggctcggagc tgcggcaacc cctggagatc accattgtcg gcggtctggt gatgagccag
    20041 ctcctgacgc tgtataccac gccggtggtg tatctctttt tcgaccgtct gcggctgcgt
    20101 ttttcgcgta aacctaaaca aacggtaacc gagtaaatga cagatcttcc cgacagcacc
    20161 cgctggcaat tatggattgt ggctttcggc ttctttatgc agtcgctgga caccaccatc
    20221 gtaaacaccg cccttccctc aatggcgcaa agcctcgggg aaagtccgtt gcatatgcac
    20281 atggtcattg tctcttatgt gctgaccgtg gcggtgatgc tgcccgccag cggctggctg
    20341 gcggacaaag tcggcgtgcg caatattttc tttaccgcca tcgtgctgtt tactctcggt
    20401 tcactgtttt gcgcgctttc cggcacgctg aacgaactgt tgctggcacg cgcgttacag
    20461 ggcgttggcg gtgcgatgat ggtgccggtc ggcaggttga cggtgatgaa aatcgtaccg
    20521 cgcgagcaat atatggcggc gatgaccttt gtcacgttac ccggtcaggt cggtccgctg
    20581 ctcggtccgg cgctcggcgg tctgctggta gaatacgcgt cgtggcactg gatctttttg
    20641 atcaacattc cggtggggat tatcggtgcg atcgccacat tgatgttaat gccgaactac
    20701 accatgcaga cgcggcgctt tgatctttcc ggctttttat tgctggcggt tggcatggcg
    20761 gtattaaccc tggcgctgga cggcagtaaa ggtacaggtt tatcgccgct ggcgattgct
    20821 ggcctggtcg cagttggcgt ggtggcactg gtgctttatc tgctgcacgc cagaaataac
    20881 aaccgcgccc tgttcagtct gaaactgttc cgcactcgta ccttttcgct gggcctggcg
    20941 gggagctttg ccggacgtat tggcagtggc atgttgccct ttatgacacc ggttttcctg
    21001 caaattggcc tcggtttctc gccgtttcat gccggactga tgatgatccc gatggtgctt
    21061 ggcagcatgg gaatgaagcg aattgtggta caggtggtga atcgctttgg ttatcgtcgg
    21121 gtactggtag cgaccacgct gggtctgtcg ctggtcaccc tgttgtttat gactaccgct
    21181 ctgttgggct ggtactacgt tttgccgttc gtcctgtttt tacaagggat ggtcaactcg
    21241 acccgtttct cctccatgaa caccctgacg ctgaaagatc tcccggacaa tctggcgagc
    21301 agcggaaaca gcctgctgtc gatgattatg caattgtcga tgagtatcgg cgtcaccatc
    21361 gccgggctgt tgctggggct ttttggttcg cagcatatca gcgtcgacag cggcaccaca
    21421 caaaccgtct ttatgtacac ctggcttagc atggcgttta tcatcgccct tccggcgttc
    21481 atctttgcca gagtgccgaa cgatacgcat caaaatgtag ctatttcgcg gcgaaaaagg
    21541 agcgcgcaat gaagttctgg cgacccggta ttaccggcaa actgtttctg gcgattttcg
    21601 ccacctgcat tgtcttgctg atcagtatgc actgggcggt gcgtatcagt tttgagcgtg
    21661 gctttattga ttacatcaaa cacggcaatg aacagcgatt acagctgtta agtgatgcgc
    21721 ttggcgagca gtatgcgcag catggtaact ggcgcttcct gcgcaacaac gatcgctttg
    21781 tctttcagat cctgcgttca tttgaacacg ataattcgga agataaaccc ggcccgggta
    21841 tgccgccgca cggctggcgt acccagttct gggtggtcga tcaaaacaac aaagtactgg
    21901 ttggtccgcg agcgccgatt ccacctgacg gtacacggcg acccattctg gtcaacggtg
    21961 cggaagtggg cgcggtgatt gcctcccctg ttgagcggct gacccgcaat actgatatca
    22021 atttcgataa acaacagcgg caaaccagct ggctgatcgt cgccctggca acgttactcg
    22081 cggcgctcgc cacctttctg ctggcgcgcg gtttgctggc accggtaaaa cgacttgtcg
    22141 atggcacgca caaactggcg gcgggcgatt tcactacccg cgtaacgccc accagtgaag
    22201 atgaactggg caaactggcg caagacttca accagctcgc cagcacgctg gagaaaaacc
    22261 agcaaatgcg gcgcgatttt atggccgata tttctcacga actgcgtacg ccattagcgg
    22321 tgctgcgcgg tgaactggaa gccattcagg atggcgtgcg caaattcacg ccggagacgg
    22381 tggcgtcttt acaggcggag gtcggtacac tgaccaaact ggttgacgat cttcatcagt
    22441 tgtcgatgtc tgatgaaggc gctctcgcct atcaaaaagc accggtagat ttgatcccac
    22501 tgctggaagt ggcgggcggc gcatttcgcg aacgattcgc cagccgtggc ctgaaactgc
    22561 aattttccct gccagacagt atcaccgtat ttggcgatcg cgaccgttta atgcagttat
    22621 tcaataactt actggaaaac agcctgcgct acactgacag cggcggcagc ctgaaaatct
    22681 ctgccgagca gcacgacaaa acggtgcgcc tgacctttgc cgacagtgcg ccaggtgtca
    22741 gtgacgatca gctacaaaaa ttgtttgaac gtttttatcg caccgaaggt tcccgcaacc
    22801 gcgccagcgg cggttccggg ctggggctgg cgatttgcct gaacattgtt gaagcccata
    22861 atggccgcat tattgccgcc cattcgcctt ttggcggggt aagcattaca gtagagttac
    22921 cgctggaacg ggatttacag agagaagtat gaccgagtta ccaatcgacg aaaacacacc
    22981 gcgtattttg atcgtggaag atgaaccgaa gctggggcag ttgctcattg attatctgcg
    23041 tgctgcgagc tatgcgccga cgcttatcag ccacggcgat caggtactgg cgtatgtgcg
    23101 ccagacacca ccggatctga tcctgttgga tctgatgctc cctggcaccg atggcctgac
    23161 attatgccgg gaaattcgtc gtttttccga tgttccgatc gtgatggtga cggcaaaaat
    23221 cgaagagatc gaccgcctgc tggggctgga gattggcgcc gatgactaca tctgcaagcc
    23281 gtacagccca cgggaagtgg tagcgcgtgt caaaaccatt ttgcgccgtt gtaaaccgca
    23341 acgcgaactt cagcaacagg atgccgaaag cccgttgatt atcgacgaag gtcgttttca
    23401 ggcttcatgg cgcggtaaaa tgcttgacct gacgcctgcg gaatttcgtc tgcttaaaac
    23461 gctctctcac gaaccaggaa aagtgttctc ccgcgagcaa ttgctcaatc atctttatga
    23521 cgactaccgc gtagtaaccg accgcaccat tgacagccac attaaaaatc tgcgccgcaa
    23581 gctggaatct ctcgacgccg aacagtcatt tatccgcgcc gtttacggcg ttggttaccg
    23641 ctgggaagcc gacgcctgcc gcatcgttta gttttagcga cattactttg ttagccggag
    23701 atggcgttca gacccgatcc ggcaacatta ttccacgcat ggtcagcaaa ctgacattat
    23761 ctctccgccc tggcaggcat cagaaaatcg ctcatacttt aatcagtaaa cagcaccttt
    23821 agatgctgtt ttcgataagc aatttcaatc aaggagtcat tatggctggt tggtttgaac
    23881 tcagtaagag cagtgataat cagttccggt ttgtgctaaa agcgggcaat ggtgagacta
    23941 tcctcaccag cgagctgtat acctcaaaag cctctgcgga aaagggcatc gcgtcggtgc
    24001 gtagcaacag cccgcaagaa gagcgctatg agaaaaaaac ggcaagcaac ggcaaattct
    24061 atttcaatct gaaagccgct aatcatcaaa ttatcggctc cagccagatg tacgccaccg
    24121 cgcaatctcg tgaaaccgga attgcctccg ttaaagccaa tggcacaagc cagacggtga
    24181 aagacaatac gtaattacga tgccgggcgt gggttgttgg cgtccggcgc tttaccagat
    24241 cccccctaca gcgctacaat gcccgccctt aaagtggggg cctcccctaa ccgcttcatc
    24301 aggtgaagcg gatctgacct gtcatcagaa cgagagaatt atgtttaaac cggaactcct
    24361 ttccccggcg ggaacgctga aaaatatgcg ttacgctttc gcttatggcg cagatgctgt
    24421 ttatgcgggc cagccgcgtt actccctacg tgtgcgcaac aacgaattca accacgaaaa
    24481 tcttcagctc ggcatcaatg aagcccacgc gctggggaaa aagttttatg tcgtggtcaa
    24541 cattgcaccg cacaacgcca agctgaaaac ctttatccgt gacctgaaac cggtggtgga
    24601 aatggggccg gatgcgctga ttatgtccga tccagggctg attatgctgg tgcgtgagca
    24661 cttccctgaa atgccgatcc acctctcggt gcaggctaac gccgtgaact gggcgacggt
    24721 gaaattctgg cagcaaatgg gactgacccg cgtgatcctc tctcgcgagc tgtcgctgga
    24781 agagattgaa gagatccata atcaggtgcc ggatatggag atcgagatct tcgttcacgg
    24841 cgcactgtgc atggcctact ccggtcgctg cctgctctct ggctatatca acaagcgcga
    24901 cccgaaccag ggcacctgca ccaacgcctg ccgctgggag tacaacgtcc aggaagggaa
    24961 agaagatgac gttggcaaca tcgtacacaa gtacgagccg attccggtgc aaaatgttga
    25021 gccgacgctg ggtatcggcg cgccaaccga caaagtgttt atgatcgaag aagcccagcg
    25081 tccgggcgag tatatgaccg cgtttgaaga tgagcacggc acttacatca tgaactcgaa
    25141 agatctgcgc gccatcgccc atgtagaacg cctgaccaaa atgggcgtgc attcgctgaa
    25201 aatcgaaggt cgtaccaaat ctttctacta ttgcgcacgc accgcacagg tttaccgcaa
    25261 agctatcgat gacgccgctg cgggaaaacc gttcgatacc agcctgctgg aaactctgga
    25321 aggtctggcg catcgtggct ataccgaagg tttcctgcgt cgtcatactc acgacgatta
    25381 tcagaactac gaatacggtt attcagtttc tgaccgccag cagtttgttg gtgagtttac
    25441 cggtgagcgc aagggggacc tcgcagcggt agcggtgaaa aataaattct ccgttggcga
    25501 cagtcttgag ctgatgacgc cgcaaggcaa cattaacttt acccttgagc acatggaaaa
    25561 cgccaaaggc gaagctatgc cggtcgcacc aggcgatggt tatactgtgt ggctcccggt
    25621 cccgcaggat cttgagctaa attacgcgct gctaatgcgt aatttctccg gggaaaccac
    25681 gcgtaacccc cacggtaagt gattaatttc gattattttt cccggatgga aaattcttag
    25741 aaaccgatca catacagctg catttattaa ggttatcatc cgtttcgctg aaaaacataa
    25801 tccataaaat gctagctgta ccaggaacca cctccttagc ctgtgtaatc tcccttacac
    25861 gggcttattt tttgtacata agctgtaaag aaggattgat ccctgataac cgtcttccat
    25921 tgaccacatc gatagaatcc cccttcatag cacaatgcct ttcacgtaac ggtatcgtgc
    25981 tcgcccaggt tccggctacg cacagccaga acgcgcatgt ttgacgctta caaaaaaata
    26041 ttctcactct ccacatttga atgtcagacg agcgacgcca tgtaatcctg caccttctgt
    26101 cttcaggtca actatctgca tttttttgcc ctgagtaaca cagaaatggg ctgcatcatt
    26161 ttttactata ttttctgcac cagatattct gcccctggct aaagaagctt cggcttcggt
    26221 gtagtattgg ttatcgagtt tacgctgaat attactttta tatgcaagac caaatttacc
    26281 gatacttgtt tcatcattat gcacagcaca accagatata agaaaaatac taattaatga
    26341 tatagcagct atctttttca tctcaccttc ccccattaaa taccaacgac actctctagt
    26401 gtttaaatat aataatggca tgattattat aattgaatag gattataata aatgttctgt
    26461 acaacatttc ctacataagt aggaattacg gacattgagg cccttcaggg taactccatg
    26521 ggggctttaa tatattatat tgaagatgcc actgtttagt tgaatattag gtatatgctc
    26581 ttttttgaaa tttatcggtg gcagccgtta gtattcgctg tccccattgc aagctcctgg
    26641 tggtaaccac tgaatcctcc atacttgaac tgacttttta tcctccgact ttcatcctgt
    26701 tctgactcca ccttttgttt tctgctctac actatctaca gaccaatcat aaaggcacat
    26761 acgatcatgg cagaatttcc cgccagctta ctgattctta atggtaaaag tactgacaat
    26821 ctacccttgc gcgaagcaat tatgctgttg cgtgaggaag gaatgacgat ccatgtgcgg
    26881 gtcacctggg agaaaggtga tgccgcacga tttgtagagg aggcccggaa gttgggcgtc
    26941 gcaacggtga ttgccggtgg tggcgatggc accattaatg aagtttctac ggcgttgatt
    27001 cagtgtgagg gggatgacat acccgcgctg ggaattttgc cattaggaac cgccaatgat
    27061 tttgccacca gtgtagggat tcctgaggca ctggataagg cgctgaaact ggcaattgcc
    27121 ggtaatgcca ttgcgataga tatggcgcag gtcaacaaac aaacctgttt tattaatatg
    27181 gcgacaggcg gatttgggac gcgtattacc acagaaacgc cggaaaaatt aaaagccgcg
    27241 ctgggtggcg tctcttacat cattcatggc ttaatgcgca tggatactct gcaaccggac
    27301 cgttgtgaaa tccgcggtga aaactttcac tggcaaggtg acgccctggt cattggtatt
    27361 ggtaacgggc gtcaggccgg tggcggtcaa caattgtgcc cgaacgcgtt aattaacgat
    27421 ggcttgctgc aactgcgcat ttttaccggc gatgaaattc ttccggctct cgtatcaacc
    27481 ttaaaatctg acgaagataa cccgaatatt atcgaaggcg cttcgtcgtg gtttgatata
    27541 caagccccac acgaaatcac ttttaatctt gatggcgaac cgttgagtgg gcaaaacttc
    27601 catattgaaa tacttccggc ggcgttgcgt tgtcgattac caccagattg tccattattg
    27661 cgttaatcaa actattctct cttgaaccag ggagaatccg taatgccgga tgcggtgtaa
    27721 acaccttatc cggcatacag aacaatacta caccgtaatt aattttaccc gtgtcttctg
    27781 caatgccaat ttatccacct ctgacacagc atcatcggta acaataatgt caattttttc
    27841 gattggcagt acctgattaa aaccgcgacg attaaatttc gatgaatcga gaacggcaat
    27901 cactttattt gcggcggtga ccatcgcccc gctaatcgaa taaccttcat taaaggtcgt
    27961 aataccatta accgcatcaa taccatcagc accgacaaac attaaatcgg cattaatatc
    28021 ctgcaatgaa cgctcggcaa tggaaccatg catcgagcgc gttttatggc ggaccgtgcc
    28081 accacagacg accagagtaa tgtctttatt ttcggaaagg gcaaacgctg ccgggagact
    28141 gttggtaatc accgtgatat ttttagcggt cattaatcct tcggcgataa gcattgtggt
    28201 acttccgctg tcgagaataa cggtcatccc ttcatggatc attttgactg ccgcctgcgc
    28261 tattctgttt tttggcgcgc tggcgagctg aaatcgctct ttaaacccga cttcctgggt
    28321 ctcggtttca ctattaccgg acattatttt cgccgcaccg ccatgaaagc gcgtaaccac
    28381 gcctttttgt tcgagaaagc gcaaatcggc acggattgtc gcttccgagg cagcaaatac
    28441 tcccgccaga tcctgaacaa gcacggttcc ctgttcattc actaattgga tgatcttatt
    28501 ccttcgctcg aatgaattca tcgtcattta cccatattca attgtggcta gtgtaaacga
    28561 agggggacca ttggtgaatg atctgttttg cacaataccg ggccagcatc acgctggccc
    28621 gcggtttcat ggaatgagca acactttgcc ctgcatggca ttacgagcga tgtcacgcac
    28681 cgcctgggtg aagctttcaa agcttccacg gtgagcgatt aatggctcca ggcttaactt
    28741 acgttctgtc agcaaccggc tcgccgtttc ccactcctgc cccggccaag ggctggaata
    28801 gttcatccaa ctgccgataa ccgtcagctc tttacgcaat attttgccaa acgttgccga
    28861 tgttaaatgc agatcctgat gcaacgtgcc caccagcgcc agttgggcat gaggaccggc
    28921 aatctctacc gccagttcga cagtttgcgg tacgccagcc gtctcgagga taagctgatt
    28981 aaagcgcagt tcgcgtaaaa cgctctgcat ttgcggcgcg ctcatttcgc tactgttaaa
    29041 tgtttgcatc gcaccgaaag attttgccag tgccagtttt tctgagctaa tgtcgatcgc
    29101 cgtcacactc tttgctccca gcgcgacagc gcactgaata gccagcaggc caatggttcc
    29161 ggcaccaata ataataacgt ttttattctc acaaccttgc gctaaatgaa aagcatgcag
    29221 gccaacggta atcggctcaa taaaagcccc atcctcaata ggcatatccg tgggtagagc
    29281 aaagacattt tttcgcttaa cgacaatata ttcagcaaat ccaccatcac gccgcgagcc
    29341 aataaaatca tattttgcgc actgggaata aaaccctttc aaacactctg gacaagtaaa
    29401 acagggtaat aacggcacac aggcaaccgc atcgccaggg tgtaaatcat caacaccgga
    29461 tcccaccgca tcaatatagc cgctaaattc atggcctaac gttattggat aataatgtgc
    29521 accattttta aatatcctgg gtaaatcgga accacataag cccgagctgg caatttttac
    29581 ccgcacctca tcctgatgtt taatttcagg aatgacgctt tttgcaacgc gcacgatacc
    29641 atcagtatca ttcactactg atttcataaa aactcctgat t
//
LOCUS       DAJTYM010000044        29603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTYM010000044 DAJTYM010000000
VERSION     DAJTYM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO575_001997"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO575_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6664972.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO575_001998"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO575_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6664973.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO575_001999"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO575_001999"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6664974.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO575_002000"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO575_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6664975.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO575_002001"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO575_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6664976.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO575_002002"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO575_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6664977.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO575_002003"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO575_002003"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6664978.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO575_002004"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO575_002004"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6664979.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO575_002005"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO575_002005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6664980.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO575_002006"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO575_002006"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6664981.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO575_002007"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO575_002007"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6664982.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO575_002008"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO575_002008"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6664983.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO575_002009"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO575_002009"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6664984.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO575_002010"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO575_002010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6664985.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO575_002011"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO575_002011"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6664986.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO575_002012"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO575_002012"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6664987.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO575_002013"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO575_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6664988.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO575_002014"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO575_002014"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6664989.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19508..20329
                     /locus_tag="OO575_002015"
     CDS             19508..20329
                     /locus_tag="OO575_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6664990.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20316..22424
                     /locus_tag="OO575_002016"
     CDS             20316..22424
                     /locus_tag="OO575_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6664991.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22421..24088
                     /locus_tag="OO575_002017"
     CDS             22421..24088
                     /locus_tag="OO575_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6664992.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24091..25617
                     /locus_tag="OO575_002018"
     CDS             24091..25617
                     /locus_tag="OO575_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR6664993.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25618..27234
                     /locus_tag="OO575_002019"
     CDS             25618..27234
                     /locus_tag="OO575_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6664994.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27290..27442)
                     /locus_tag="OO575_002020"
                     /pseudo
     CDS             complement(27290..27442)
                     /locus_tag="OO575_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27465..27842)
                     /locus_tag="OO575_002021"
     CDS             complement(27465..27842)
                     /locus_tag="OO575_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664995.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28252..28623)
                     /locus_tag="OO575_002022"
     CDS             complement(28252..28623)
                     /locus_tag="OO575_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664996.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28684..29181)
                     /locus_tag="OO575_002023"
     CDS             complement(28684..29181)
                     /locus_tag="OO575_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6664997.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29257..>29603)
                     /locus_tag="OO575_002024"
     CDS             complement(29257..>29603)
                     /locus_tag="OO575_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HCR6664998.1"
                     /translation="AAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSR
                     IWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEDRLASRADQLEEFVH
                     YVKGEITKVVGK"
BASE COUNT         7627 a         6930 c         7620 g         7426 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc
    19381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
    19441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
    19501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
    19561 agaggggcgt ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt
    19621 tccttcttca ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt
    19681 gctatctgac ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga
    19741 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
    19801 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
    19861 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
    19921 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
    19981 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
    20041 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
    20101 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
    20161 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
    20221 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
    20281 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
    20341 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
    20401 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
    20461 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
    20521 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
    20581 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
    20641 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
    20701 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
    20761 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
    20821 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
    20881 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
    20941 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
    21001 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
    21061 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
    21121 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
    21181 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
    21241 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
    21301 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
    21361 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
    21421 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
    21481 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
    21541 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
    21601 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
    21661 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
    21721 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
    21781 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
    21841 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
    21901 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
    21961 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
    22021 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
    22081 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
    22141 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
    22201 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
    22261 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
    22321 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
    22381 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
    22441 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
    22501 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
    22561 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
    22621 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
    22681 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa
    22741 tggttatgag cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc
    22801 tacggcacaa agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca
    22861 tcgtattcta gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt
    22921 ggtgtatttg aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt
    22981 tttcagagcg ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg
    23041 tcatggtata gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg
    23101 gttgttggtt attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga
    23161 gatgctgaac ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg
    23221 tacccctaaa gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg
    23281 gtttggagct atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga
    23341 gtggatcgct tttacggata atctttggca attacagctt ttacaacgca aagatgcgct
    23401 gttatcggat gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt
    23461 agtaaaactt tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac
    23521 cgctggttta ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga
    23581 ggcattacgc tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga
    23641 gattgataaa cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc
    23701 agaagaaaaa gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct
    23761 gaaagaggat tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa
    23821 tccaacgatg acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga
    23881 aacggtagtg tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt
    23941 agtcaagccc agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca
    24001 acgccaacct gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat
    24061 ggcgagctta tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat
    24121 gagctgattt atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag
    24181 cagctgttgg atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg
    24241 catttaggtg tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt
    24301 atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac
    24361 gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc
    24421 gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt
    24481 cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca
    24541 tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat
    24601 caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct
    24661 caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag
    24721 ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg
    24781 ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt
    24841 agtgatgagg cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg
    24901 ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg
    24961 tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt
    25021 actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat
    25081 ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg
    25141 gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat
    25201 tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt
    25261 gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta
    25321 gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac
    25381 ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt
    25441 tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt
    25501 aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa
    25561 gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg
    25621 gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt
    25681 aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa
    25741 tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca
    25801 aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc
    25861 aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg
    25921 cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca
    25981 cagttagagc tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg
    26041 agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta
    26101 gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta
    26161 gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt
    26221 tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc
    26281 tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag
    26341 gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc
    26401 acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag
    26461 cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag
    26521 gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac atggataagt
    26581 tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt
    26641 ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca
    26701 cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac
    26761 aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact
    26821 aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc
    26881 cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt
    26941 aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct
    27001 accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg
    27061 cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga
    27121 caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga
    27181 gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat
    27241 gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc
    27301 catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga
    27361 tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg
    27421 attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg
    27481 tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg
    27541 gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt
    27601 gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga
    27661 aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa
    27721 gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca
    27781 tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac
    27841 acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt
    27901 cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt
    27961 gatatttgaa acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc
    28021 ttgtaaatat cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta
    28081 tagaaatcgt cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca
    28141 tcgccatacc ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga
    28201 gcttgtacgc aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc
    28261 catccattcg gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta
    28321 tgttgcctgc accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc
    28381 ccacttgccc acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc
    28441 ctcctttaac tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc
    28501 gacatagtct ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt
    28561 gtcatacatg acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga
    28621 cactagtggt tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc
    28681 aggctagttg cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg
    28741 gccatttatg acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg
    28801 cccccctttt ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc
    28861 attgttggca atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag
    28921 cctacgattt ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc
    28981 agagttgtcg taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct
    29041 tggagaaatg tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa
    29101 aacaattggc aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac
    29161 caccttcaac agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc
    29221 cgcgaagcgg cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat
    29281 ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc
    29341 ttcttgtcca agataagcct gcctagcttc aagtatgacg ggctgatact gggccggcag
    29401 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
    29461 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
    29521 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
    29581 atcaaagagt tcctccgccg ctg
//
LOCUS       DAJTYM010000045        29077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTYM010000045 DAJTYM010000000
VERSION     DAJTYM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..311)
                     /locus_tag="OO575_002025"
                     /pseudo
     CDS             complement(1..311)
                     /locus_tag="OO575_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(286..1086)
                     /gene="otsB"
                     /locus_tag="OO575_002026"
     CDS             complement(286..1086)
                     /gene="otsB"
                     /locus_tag="OO575_002026"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6664999.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1253..2239)
                     /gene="araH"
                     /locus_tag="OO575_002027"
     CDS             complement(1253..2239)
                     /gene="araH"
                     /locus_tag="OO575_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6665000.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2254..3768)
                     /gene="araG"
                     /locus_tag="OO575_002028"
     CDS             complement(2254..3768)
                     /gene="araG"
                     /locus_tag="OO575_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6665001.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3838..4827)
                     /gene="araF"
                     /locus_tag="OO575_002029"
                     /pseudo
     CDS             complement(3838..4827)
                     /gene="araF"
                     /locus_tag="OO575_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5624..6127
                     /locus_tag="OO575_002030"
     CDS             5624..6127
                     /locus_tag="OO575_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6665002.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6207..6458)
                     /locus_tag="OO575_002031"
     CDS             complement(6207..6458)
                     /locus_tag="OO575_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6665003.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6573..6659)
                     /gene="azuC"
                     /locus_tag="OO575_002032"
     CDS             complement(6573..6659)
                     /gene="azuC"
                     /locus_tag="OO575_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6665004.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            6922..7245
                     /locus_tag="OO575_002033"
     CDS             6922..7245
                     /locus_tag="OO575_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6665005.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7416..7913
                     /gene="ftnA"
                     /locus_tag="OO575_002034"
     CDS             7416..7913
                     /gene="ftnA"
                     /locus_tag="OO575_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6665006.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(7950..8189)
                     /locus_tag="OO575_002035"
     CDS             complement(7950..8189)
                     /locus_tag="OO575_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6665007.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8379..9590
                     /gene="tyrP"
                     /locus_tag="OO575_002036"
     CDS             8379..9590
                     /gene="tyrP"
                     /locus_tag="OO575_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6665008.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9652..10317)
                     /gene="yecA"
                     /locus_tag="OO575_002037"
     CDS             complement(9652..10317)
                     /gene="yecA"
                     /locus_tag="OO575_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6665009.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10513..10599)
                     /locus_tag="OO575_002038"
     tRNA            complement(10513..10599)
                     /locus_tag="OO575_002038"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10563..10565),aa:Leu,seq:taa)
     gene            complement(10612..10685)
                     /locus_tag="OO575_002039"
     tRNA            complement(10612..10685)
                     /locus_tag="OO575_002039"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10651..10653),aa:Cys,seq:gca)
     gene            complement(10740..10815)
                     /locus_tag="OO575_002040"
     tRNA            complement(10740..10815)
                     /locus_tag="OO575_002040"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10780..10782),aa:Gly,seq:gcc)
     gene            complement(10967..11515)
                     /gene="pgsA"
                     /locus_tag="OO575_002041"
     CDS             complement(10967..11515)
                     /gene="pgsA"
                     /locus_tag="OO575_002041"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6665010.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11572..13404)
                     /gene="uvrC"
                     /locus_tag="OO575_002042"
     CDS             complement(11572..13404)
                     /gene="uvrC"
                     /locus_tag="OO575_002042"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6665011.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13401..14057)
                     /gene="uvrY"
                     /locus_tag="OO575_002043"
     CDS             complement(13401..14057)
                     /gene="uvrY"
                     /locus_tag="OO575_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6665012.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14353..14529
                     /locus_tag="OO575_002044"
     CDS             14353..14529
                     /locus_tag="OO575_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665013.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14516..14740
                     /locus_tag="OO575_002045"
     CDS             14516..14740
                     /locus_tag="OO575_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6665014.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14773..15393)
                     /gene="sdiA"
                     /locus_tag="OO575_002046"
                     /pseudo
     CDS             complement(14773..15393)
                     /gene="sdiA"
                     /locus_tag="OO575_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15623..16375)
                     /gene="yecC"
                     /locus_tag="OO575_002047"
     CDS             complement(15623..16375)
                     /gene="yecC"
                     /locus_tag="OO575_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6665015.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16372..17040)
                     /gene="tcyL"
                     /locus_tag="OO575_002048"
     CDS             complement(16372..17040)
                     /gene="tcyL"
                     /locus_tag="OO575_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6665016.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17055..18041)
                     /gene="dcyD"
                     /locus_tag="OO575_002049"
     CDS             complement(17055..18041)
                     /gene="dcyD"
                     /locus_tag="OO575_002049"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6665017.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18146..18946)
                     /gene="tcyJ"
                     /locus_tag="OO575_002050"
     CDS             complement(18146..18946)
                     /gene="tcyJ"
                     /locus_tag="OO575_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6665018.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19034..19585)
                     /gene="fliZ"
                     /locus_tag="OO575_002051"
     CDS             complement(19034..19585)
                     /gene="fliZ"
                     /locus_tag="OO575_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6665019.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19631..20350)
                     /gene="fliA"
                     /locus_tag="OO575_002052"
                     /pseudo
     CDS             complement(19631..20350)
                     /gene="fliA"
                     /locus_tag="OO575_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20515..22167)
                     /gene="fliC"
                     /locus_tag="OO575_002053"
     CDS             complement(20515..22167)
                     /gene="fliC"
                     /locus_tag="OO575_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6665020.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22433..23839
                     /gene="fliD"
                     /locus_tag="OO575_002054"
     CDS             22433..23839
                     /gene="fliD"
                     /locus_tag="OO575_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6665021.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23864..24274
                     /gene="fliS"
                     /locus_tag="OO575_002055"
     CDS             23864..24274
                     /gene="fliS"
                     /locus_tag="OO575_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6665022.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24274..24639
                     /gene="fliT"
                     /locus_tag="OO575_002056"
     CDS             24274..24639
                     /gene="fliT"
                     /locus_tag="OO575_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6665023.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24717..26204
                     /gene="amyA"
                     /locus_tag="OO575_002057"
     CDS             24717..26204
                     /gene="amyA"
                     /locus_tag="OO575_002057"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6665024.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26238..26651)
                     /locus_tag="OO575_002058"
     CDS             complement(26238..26651)
                     /locus_tag="OO575_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6665025.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26838..28043
                     /gene="yedE"
                     /locus_tag="OO575_002059"
     CDS             26838..28043
                     /gene="yedE"
                     /locus_tag="OO575_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6665026.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28040..28273
                     /gene="yedF"
                     /locus_tag="OO575_002060"
     CDS             28040..28273
                     /gene="yedF"
                     /locus_tag="OO575_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6665027.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28380..29050
                     /locus_tag="OO575_002061"
                     /pseudo
     CDS             28380..29050
                     /locus_tag="OO575_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         7246 a         7497 c         6924 g         7410 t
ORIGIN      
        1 tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat aatgaaaagc
       61 gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct gttcgctgag
      121 gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt ccctgtttca
      181 ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat gccaacggca
      241 aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga tactacgact
      301 aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg cggcggttat
      361 catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg cctgagttgc
      421 acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga agccagattc
      481 atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag cttcctgcat
      541 aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga caacacactt
      601 tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta atgtcattaa
      661 tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc ctttcgcctc
      721 cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa tatcacgcgc
      781 aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc gctccgcccc
      841 atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct ccaccattga
      901 gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct gtagtccttg
      961 cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg ccagcgttcc
     1021 atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg ttaacggttc
     1081 tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc gctttacaga
     1141 catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca gccaaccgtt
     1201 gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag catcagacag
     1261 tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg attaagccgc
     1321 gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt tccacggtgc
     1381 ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct ttcagagaaa
     1441 cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac gtcattggct
     1501 gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc aggccagaga
     1561 gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc gcagcctctt
     1621 cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc agcaaaccaa
     1681 agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac cagttagcgt
     1741 aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct gaaatgatgt
     1801 acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca tttattttca
     1861 gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac aaacccgctg
     1921 ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg acacccgcac
     1981 aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag aacaacatgc
     2041 cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata ttaatgaagg
     2101 tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac accaccagca
     2161 tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg ccagaccccg
     2221 atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc tgactttagg
     2281 catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt caccggcgat
     2341 ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag gtaagtcgct
     2401 ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt tatatatttc
     2461 gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga ccttcatctc
     2521 ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga gattcatgat
     2581 cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat cggcattgtt
     2641 ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc tggcactgat
     2701 gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc gagcttccgg
     2761 gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga tcggctgttg
     2821 gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt tcattaattc
     2881 gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc gaaccgccag
     2941 acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc gctcctcgcc
     3001 ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca tcgcctgcac
     3061 cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac gtccatcttt
     3121 aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag aaacgtataa
     3181 gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc cgatttcgcg
     3241 ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac gcgccagcgc
     3301 tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg gcgtgtccgg
     3361 gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca gcaatgagcg
     3421 attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg cgacggtcat
     3481 ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa gtgctgcggt
     3541 cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg ttggcgcata
     3601 gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac ccatcaacgc
     3661 atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa cgccgggaaa
     3721 cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa cgtctccgtg
     3781 aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc cggcaaatta
     3841 cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg tgatcagtac
     3901 cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc agttgtaaag
     3961 catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac cgtagaagcc
     4021 ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac caataccgat
     4081 gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa gtacggtgct
     4141 gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca gcattgagtt
     4201 ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat aaatttgttt
     4261 ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc ggcgggcggt
     4321 atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc agccacgttt
     4381 ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg ccgccatcat
     4441 caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt catccactgc
     4501 aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga gtttcgggtc
     4561 cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga tcgcgttcaa
     4621 tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat ccttcccggc
     4681 tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct tcaccagaaa
     4741 accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca gaccaatggc
     4801 tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag
     4861 ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc ttttatctaa
     4921 aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat aggagagcaa
     4981 tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa gacataagga
     5041 aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata agattagcct
     5101 ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc agtcaatagg
     5161 ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca aagatgtgga
     5221 aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta acagaacacg
     5281 tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc acttcatcat
     5341 gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat taaaattcat
     5401 tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat gatttaagca
     5461 ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa ttgactccac
     5521 gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat ggtgttttga
     5581 tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa ccgctggaat
     5641 gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc tctaccttca
     5701 cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc ttcgcgccca
     5761 ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc
     5821 tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc tggaagaact
     5881 gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc agttagccga
     5941 tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga
     6001 acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact
     6061 tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt cacaccagct
     6121 gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag aaaccgctgc
     6181 ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt aaacttgagt
     6241 tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc ggcagcggga
     6301 tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac ttcaacgggc
     6361 gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc taccacatca
     6421 gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg atagcatttt
     6481 gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac cgtcatcagg
     6541 acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc tggcggtacg
     6601 tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg cagtttcatc
     6661 gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata atatgacaac
     6721 catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt aatgttataa
     6781 aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca agaaatagcg
     6841 cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa tagtgctttc
     6901 tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt aataacgctg
     6961 gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt
     7021 gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg ttatgtcagt
     7081 cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat cccctttggt
     7141 cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac ggaattcact
     7201 ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt ttaatcagcg
     7261 aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat tcgtattttt
     7321 aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt atagtgacgc
     7381 cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa atgattgaaa
     7441 aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag caaatgagcg
     7501 cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg
     7561 aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc aatttaccgc
     7621 gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa ttattccagg
     7681 aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct catgctgcaa
     7741 tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct gagcagcatg
     7801 aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc aaaagcggcg
     7861 aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac taatgctctc
     7921 cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact ctgatcggtc
     7981 gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc taccagctct
     8041 cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc aaaacgcgct
     8101 ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat catcatattt
     8161 aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc tagcgtagcg
     8221 attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt gtacatttat
     8281 atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa cctgcgccgc
     8341 agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga accctgggaa
     8401 gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg ccgctggctg
     8461 cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg gcgttgatgt
     8521 gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat accggtctgg
     8581 gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc ttcagtatga
     8641 tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa ttgttggcct
     8701 ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca
     8761 cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt
     8821 ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg ctaccgcata
     8881 ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg tctgcaatcc
     8941 cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc agctatatgg
     9001 atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc cccctggtgg
     9061 catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc tttatgggat
     9121 tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa atggtggcct
     9181 ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc acgtcatttc
     9241 tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt tcaaataccg
     9301 ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg tttgcactgt
     9361 tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg gcggtactgg
     9421 cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct caggcgggtt
     9481 accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt attgctgtga
     9541 ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga tcagatagcc
     9601 tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct cttaatgcag
     9661 gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac gccccggttt
     9721 ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca tccagtatgc
     9781 atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg cttctggcga
     9841 cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg cctccagcgc
     9901 tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc gcatatagcc
     9961 aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa
    10021 cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa
    10081 agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca catagtcagc
    10141 cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag aactcaacac
    10201 cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag tgttgtattt
    10261 ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg
    10321 cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc gtatcagaga
    10381 tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg acaagttgca
    10441 ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt gattttattc
    10501 ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga
    10561 ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc
    10621 gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac
    10681 gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt cttttaaact
    10741 ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt gctctaccaa
    10801 ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat tatcgtctgg
    10861 caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat
    10921 atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac tgatcaagca
    10981 aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt accgcagcca
    11041 caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac agcagccagg
    11101 ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg gccacgctac
    11161 tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga
    11221 tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt accagcacca
    11281 tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca aaccgggtac
    11341 tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga
    11401 aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga
    11461 atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga
    11521 cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt
    11581 caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc
    11641 ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg
    11701 acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg
    11761 ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat gctggataac
    11821 atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct caaagaacag
    11881 cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca gcggatgatt
    11941 tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa gctggccttt
    12001 gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt taccataacg
    12061 ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt
    12121 atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac
    12181 ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa
    12241 tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt
    12301 gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg ccctatcgcc
    12361 gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag
    12421 cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg
    12481 gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca gttccgtacc
    12541 gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac gaatgaacaa
    12601 tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc
    12661 ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat
    12721 acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc taatgagttg
    12781 cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc
    12841 gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctggta
    12901 ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa
    12961 aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa
    13021 atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt gggtatcgcc
    13081 actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt aacgcggctg
    13141 atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gcgtaaccgt
    13201 tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct
    13261 gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt
    13321 accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc
    13381 ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg
    13441 cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga
    13501 tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc
    13561 ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat
    13621 gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatatcagaa
    13681 gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc ctgcggagcc
    13741 gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg taaagggttt
    13801 tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc gattttacgc
    13861 gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca
    13921 tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac gacttttata
    13981 cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca
    14041 agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt
    14101 cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga
    14161 tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc gtcaccaacg
    14221 gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat tgactttatt
    14281 cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg
    14341 caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt ttatagcact
    14401 gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat ggtatactcc
    14461 gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa aataaatgag
    14521 tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag tctcctgcct
    14581 gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat
    14641 gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg cagtattttc
    14701 caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg gctgagagcg
    14761 tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct tcccttccgc
    14821 cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg tcatcactat
    14881 ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact gcatttttaa
    14941 ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa
    15001 gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc
    15061 gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc acattaaatg
    15121 gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat tttttgcctg
    15181 ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa
    15241 tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct gagcctgaag
    15301 ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca acagcatcgt
    15361 gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc ctgagagtga
    15421 gtatcattta taatgataat tcaaactatc acataaataa gatttatata taatttatat
    15481 tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat taatgagtta
    15541 tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg acaggtgaat
    15601 cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac tggcgggtgc
    15661 gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact atccgcccct
    15721 ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg tgtgtcacaa
    15781 tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact tcgcccacca
    15841 gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga cgcattgcca
    15901 gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt ggatagctgg
    15961 tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt
    16021 ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc accgtacgat
    16081 gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac tggcgaatca
    16141 gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca acggtgatcg
    16201 ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt ttgccggaac
    16261 cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg ataccgtgca
    16321 gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca ctcattttgg
    16381 ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg ataacacagt
    16441 cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg
    16501 cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg cggccagcga
    16561 cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc
    16621 ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac tggcagcggc
    16681 ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg cggcataggc
    16741 ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt cgataccaaa
    16801 ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat
    16861 agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc gcatcagcgc
    16921 gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt gcagggtata
    16981 cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac tttcttgcat
    17041 gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc gccgccggta
    17101 tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc gtcaatcagc
    17161 cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag acgcgccagc
    17221 aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca
    17281 tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt
    17341 aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac gccaatcagt
    17401 tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt tccggcactg
    17461 cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg cgcgatttcc
    17521 agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac cggaatgaca
    17581 tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg ggcattggga
    17641 tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa caaacgattg
    17701 ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag cgccacgcag
    17761 tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga ctggatcgcg
    17821 ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag aaattccagc
    17881 ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt gatgaaaatt
    17941 tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg cgcgccgata
    18001 aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc cgatgtgtgt
    18061 tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa
    18121 gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac catttttcgg
    18181 aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc accgctttca
    18241 gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag aatgcttcac
    18301 cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc agacgatcaa
    18361 cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg tcatcatcat
    18421 aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag ttggtgccca
    18481 gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg ccttcgttac
    18541 cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa tcgtattttt
    18601 tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt ttagagtcca
    18661 gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg ccaagatgtt
    18721 ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca tccccctgaa
    18781 aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc tctttgactt
    18841 tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc agcgccacgg
    18901 ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc accccgaatg
    18961 ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca actccgctac
    19021 atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc tgcacaagtc
    19081 cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg taattgttgg
    19141 tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat
    19201 cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga acgacatatt
    19261 cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta aagccgataa
    19321 tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg
    19381 cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc aggcgggaaa
    19441 tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg agtaatttac
    19501 ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg cttaatggcc
    19561 gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta aagtgcggca
    19621 tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc tggctgtgta
    19681 actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg agattcagct
    19741 cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt tcgatggctt
    19801 ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt tctcgctgat
    19861 gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca tcgtaggaga
    19921 agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata tcgatcccta
    19981 aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc agttgcccta
    20041 ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag tcacggctgc
    20101 gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta aatgccgttc
    20161 cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc gcctgtagca
    20221 gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca
    20281 ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca gcggtataga
    20341 gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta tccgtttctg
    20401 cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg gttacgcaaa
    20461 aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag acgattaacc
    20521 ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca cggagttacc
    20581 ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat agtcggcgtc
    20641 ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc
    20701 ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga tggagctgat
    20761 ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt cagcatctgt
    20821 cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag tcactgaacc
    20881 atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt tgttatcaac
    20941 ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc tataacctgc
    21001 agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag ctgcctgatc
    21061 aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat taccggtaaa
    21121 ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg ctgcaagtgt
    21181 ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc gcccagttgt
    21241 atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc tgatctgaac
    21301 atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa ctcccggggt
    21361 caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt atgacttact
    21421 tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt tattaatggt
    21481 tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt tagcagcatc
    21541 gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag ctaccaagtc
    21601 agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa acccattcag
    21661 ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc cgtcattcgc
    21721 accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt tgaactgggt
    21781 ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct gaatggagtc
    21841 cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac ggatacgctg
    21901 taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag aaataccgtc
    21961 gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac ggttagcaat
    22021 cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag acagacgctc
    22081 gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga tcagcgagag
    22141 gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag tcgttgatta
    22201 cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt ctgtcgtaaa
    22261 acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga aatactcttt
    22321 tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt gcgcaattca
    22381 ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc gcatggcaag
    22441 tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg atagcctcac
    22501 cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt ttaccgctaa
    22561 acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg ccaatactgc
    22621 attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca ccgcgttcag
    22681 tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca cccatctggc
    22741 gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg cgatcgccac
    22801 cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta ccattgatat
    22861 cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg caaaagcggg
    22921 cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag tcacatcaaa
    22981 tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg cgctacaaag
    23041 ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg tcgccgccca
    23101 gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca ctatcagcga
    23161 cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc agacgctaac
    23221 catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga atgcctataa
    23281 ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg cgggagctga
    23341 tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga cgattcagac
    23401 gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa cgctggcgca
    23461 gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg acaaactcac
    23521 cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg atggtaaaaa
    23581 aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga caacgggcat
    23641 tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta aagactacaa
    23701 cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat ttacccaact
    23761 ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc agttcgaaaa
    23821 caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg cggcaaaagg
    23881 cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca gccaacagca
    23941 gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc gactgtttat
    24001 gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca acatcattga
    24061 gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc aaaacttaat
    24121 tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca acgatgtctc
    24181 agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga aagagtcttt
    24241 actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca tttgtatttc
    24301 gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac ggaagaacaa
    24361 tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga gatcgcacac
    24421 ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc gatgctgcac
    24481 ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat ggatgaactg
    24541 gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta tggcgatcag
    24601 ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag ccgatggctc
    24661 gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt cgaagaatgc
    24721 gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt aagctctggc
    24781 ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg gtctggttgc
    24841 cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc tatgatttat
    24901 ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt gataaagcac
    24961 aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg ctggacgtgg
    25021 tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag cgtgtaaatg
    25081 ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg acgcgttaca
    25141 ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa tgttttagcg
    25201 gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt aacgactata
    25261 ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat tatctgatgg
    25321 gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac tgggcgcgct
    25381 gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa catattccgg
    25441 catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag ccgctgttta
    25501 ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt gatcaggtgg
    25561 aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa gcatcgcgca
    25621 tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa gccgatcctt
    25681 ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc ctcgaagcac
    25741 cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg gaaaatggcg
    25801 ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc ggtggtgacg
    25861 ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta attctcgccc
    25921 gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac tgcattgcct
    25981 ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg aacggagatg
    26041 atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg cgtgattttc
    26101 tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc ttcttttgca
    26161 acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat ccccggcggc
    26221 aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc cagcgcggcg
    26281 gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta ctcaatggtg
    26341 ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt gagatcatca
    26401 gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg gcaatccaag
    26461 gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac cagcgcacgt
    26521 tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt tttcacgaca
    26581 ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc tgcaattgcg
    26641 agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt aaagctctga
    26701 tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt ttgtgaatta
    26761 ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca aaccttctga
    26821 gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg attaaattct
    26881 gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat tttggcatta
    26941 ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag ctcctgcagc
    27001 tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg gaaggatcgc
    27061 cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc tttgctgctg
    27121 cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc attatgcagg
    27181 ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc tgtaacctgg
    27241 cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt gccatcgcca
    27301 ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc cgtattcccg
    27361 tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat caggcgcggc
    27421 gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg ctgctcacgg
    27481 cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt ggtttactga
    27541 ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc accggacgta
    27601 cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg atcttcagtt
    27661 acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg gtaattggtg
    27721 gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc tggatgtacc
    27781 gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg atcggctcaa
    27841 cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac tgggacaaaa
    27901 tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat ttgctgctgt
    27961 ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt gaggcaccgc
    28021 agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg atatggtggg
    28081 tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat taaaaaaagg
    28141 tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc cactggatgc
    28201 gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca ttcgttattt
    28261 aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg ttacctctga
    28321 cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag gaggttgcta
    28381 tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt ctcgcggaag
    28441 atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat gtcgcgccgg
    28501 gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat ccggttttct
    28561 ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc gtagaaactg
    28621 cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg
    28681 atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta tctatcgcgc
    28741 tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc gtggtgacga
    28801 agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag atattcatga
    28861 ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc aagacattgg
    28921 tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc agttcacctg
    28981 gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt taattcaacc
    29041 tgtttgttga catcctccac gccctgaatg acgaggc
//
LOCUS       DAJTYM010000046        28829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTYM010000046 DAJTYM010000000
VERSION     DAJTYM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..203)
                     /locus_tag="OO575_002062"
     CDS             complement(<1..203)
                     /locus_tag="OO575_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6665028.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALK"
     gene            513..2285
                     /gene="aspS"
                     /locus_tag="OO575_002063"
     CDS             513..2285
                     /gene="aspS"
                     /locus_tag="OO575_002063"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6665029.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2403..2855
                     /gene="nudB"
                     /locus_tag="OO575_002064"
     CDS             2403..2855
                     /gene="nudB"
                     /locus_tag="OO575_002064"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6665030.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2884..3624
                     /locus_tag="OO575_002065"
     CDS             2884..3624
                     /locus_tag="OO575_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6665031.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3659..4180
                     /gene="ruvC"
                     /locus_tag="OO575_002066"
     CDS             3659..4180
                     /gene="ruvC"
                     /locus_tag="OO575_002066"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6665032.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4182..4784)
                     /locus_tag="OO575_002067"
     CDS             complement(4182..4784)
                     /locus_tag="OO575_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6665033.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4854..4919
                     /gene="yobI"
                     /locus_tag="OO575_002068"
     CDS             4854..4919
                     /gene="yobI"
                     /locus_tag="OO575_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6665034.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5058..5669
                     /gene="ruvA"
                     /locus_tag="OO575_002069"
     CDS             5058..5669
                     /gene="ruvA"
                     /locus_tag="OO575_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6665035.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5678..6688
                     /gene="ruvB"
                     /locus_tag="OO575_002070"
     CDS             5678..6688
                     /gene="ruvB"
                     /locus_tag="OO575_002070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6665036.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6835..7620)
                     /gene="znuB"
                     /locus_tag="OO575_002071"
     CDS             complement(6835..7620)
                     /gene="znuB"
                     /locus_tag="OO575_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6665037.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7617..8372)
                     /gene="znuC"
                     /locus_tag="OO575_002072"
     CDS             complement(7617..8372)
                     /gene="znuC"
                     /locus_tag="OO575_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6665038.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8451..9383
                     /gene="znuA"
                     /locus_tag="OO575_002073"
     CDS             8451..9383
                     /gene="znuA"
                     /locus_tag="OO575_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6665039.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9399..10721
                     /gene="mepM"
                     /locus_tag="OO575_002074"
     CDS             9399..10721
                     /gene="mepM"
                     /locus_tag="OO575_002074"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6665040.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10841..11812
                     /gene="lpxM"
                     /locus_tag="OO575_002075"
                     /gene_synonym="msbB"
     CDS             10841..11812
                     /gene="lpxM"
                     /locus_tag="OO575_002075"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6665041.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11943..13385)
                     /gene="pyk"
                     /locus_tag="OO575_002076"
     CDS             complement(11943..13385)
                     /gene="pyk"
                     /locus_tag="OO575_002076"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6665042.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13513..14382)
                     /gene="hexR"
                     /locus_tag="OO575_002077"
                     /gene_synonym="yebK"
     CDS             complement(13513..14382)
                     /gene="hexR"
                     /locus_tag="OO575_002077"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6665043.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14719..16194
                     /gene="zwf"
                     /locus_tag="OO575_002078"
     CDS             14719..16194
                     /gene="zwf"
                     /locus_tag="OO575_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6665044.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16429..18240
                     /gene="edd"
                     /locus_tag="OO575_002079"
     CDS             16429..18240
                     /gene="edd"
                     /locus_tag="OO575_002079"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6665045.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18277..18918
                     /gene="kdgA"
                     /locus_tag="OO575_002080"
                     /gene_synonym="eda"
     CDS             18277..18918
                     /gene="kdgA"
                     /locus_tag="OO575_002080"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6665046.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18974..20152)
                     /gene="purT"
                     /locus_tag="OO575_002081"
     CDS             complement(18974..20152)
                     /gene="purT"
                     /locus_tag="OO575_002081"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6665047.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20286..20576
                     /gene="yebG"
                     /locus_tag="OO575_002082"
                     /pseudo
     CDS             20286..20576
                     /gene="yebG"
                     /locus_tag="OO575_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20643..20999
                     /locus_tag="OO575_002083"
     CDS             20643..20999
                     /locus_tag="OO575_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6665048.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21326..21985
                     /locus_tag="OO575_002084"
     CDS             21326..21985
                     /locus_tag="OO575_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6665049.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22195..24255
                     /gene="ptrB"
                     /locus_tag="OO575_002085"
     CDS             22195..24255
                     /gene="ptrB"
                     /locus_tag="OO575_002085"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6665050.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24252..24914)
                     /gene="exoX"
                     /locus_tag="OO575_002086"
     CDS             complement(24252..24914)
                     /gene="exoX"
                     /locus_tag="OO575_002086"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6665051.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24938..25594)
                     /locus_tag="OO575_002087"
                     /pseudo
     CDS             complement(24938..25594)
                     /locus_tag="OO575_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25696..25926)
                     /gene="holE"
                     /locus_tag="OO575_002088"
     CDS             complement(25696..25926)
                     /gene="holE"
                     /locus_tag="OO575_002088"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6665052.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26065..26439
                     /gene="yobA"
                     /locus_tag="OO575_002089"
     CDS             26065..26439
                     /gene="yobA"
                     /locus_tag="OO575_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6665053.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTNT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26443..27315
                     /gene="copD"
                     /locus_tag="OO575_002090"
                     /pseudo
     CDS             26443..27315
                     /gene="copD"
                     /locus_tag="OO575_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27328..27669
                     /locus_tag="OO575_002091"
     CDS             27328..27669
                     /locus_tag="OO575_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6665054.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28062..28829)
                     /locus_tag="OO575_002092"
                     /pseudo
     CDS             complement(28062..28829)
                     /locus_tag="OO575_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         7295 a         7098 c         7439 g         6997 t
ORIGIN      
        1 tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa cacgggctga
       61 ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta
      121 tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac caacgcagtg
      181 gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg cctcacacct
      241 taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac
      301 acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg
      361 cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt
      421 ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg
      481 catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt ggacagctcc
      541 gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc
      601 ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg
      661 atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag ttctgcattc
      721 aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg
      781 gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat gttctgccgc
      841 ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac ctcgacctgc
      901 gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc
      961 gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc aaagccacgc
     1021 cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcac
     1081 tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt gaccgttact
     1141 atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta
     1201 ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag
     1261 cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat ttcccggtaa
     1321 tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg cgtaacccga
     1381 tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta tttgcggggc
     1441 cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc gcatcgctga
     1501 cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg
     1561 cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga
     1621 agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg caagatggcg
     1681 atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg ggcgcactgc
     1741 tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca ccgctgtggg
     1801 ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca atgcaccatc
     1861 cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg gaaaatgcgg
     1921 tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt tcagtacgta
     1981 tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac gaagaggaac
     2041 agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg ccgcacgcag
     2101 gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac aatatccgtg
     2161 acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa gcaccgagct
     2221 ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag gctgagaata
     2281 actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca actgagtgaa
     2341 ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa cgaagaggca
     2401 gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc tatgcacaag
     2461 atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg cagtcggtaa
     2521 ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag
     2581 aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag cgcacggtag
     2641 agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg cgtaatacgg
     2701 aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact gaacatctgg
     2761 cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg agcaaccggc
     2821 aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc tggagatatt
     2881 tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc gcaggatgct
     2941 aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc taagctgggc
     3001 ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc actgtctaac
     3061 aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat
     3121 gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc aatcatgatt
     3181 gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc attcagcaaa
     3241 tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa aaaaggcgtg
     3301 atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga agcaggtgct
     3361 gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg ggaagaaatg
     3421 ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc
     3481 atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact gatgcgtctg
     3541 atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg tgaaatctct
     3601 gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga gacgcgtgat
     3661 ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg tcatccgcca
     3721 ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag tggatgattt
     3781 accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc agttccagcc
     3841 tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag ccctgaaact
     3901 gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc cggtatttga
     3961 atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg aaaaaagcca
     4021 ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg cggatgccgc
     4081 cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga tgcagatgag
     4141 cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc ctcggtcagt
     4201 tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg atgatgccac
     4261 aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa ccagaatttc
     4321 cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt ctctatttca
     4381 cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga acagaactgg
     4441 cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga gggaacctgt
     4501 tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt tatagcatag
     4561 cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga aaggggaacc
     4621 cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc gacgtgatta
     4681 ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat acatgtaaag
     4741 acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc attccattga
     4801 aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc gcaatgtata
     4861 tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt ccaatataac
     4921 ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg caagttttct
     4981 tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat ctttgattca
     5041 ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa aaacaacccc
     5101 cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg atgacctgtt
     5161 tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg
     5221 aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg ttcaaagagt
     5281 tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc ggaatgtcag
     5341 cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg aaactgccgg
     5401 gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt
     5461 tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct gctagcccgg
     5521 cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc tataaaccac
     5581 aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt gaaacattaa
     5641 ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag accgtctgat
     5701 ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc ctaaattact
     5761 ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca tcaaagcagc
     5821 gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg ggttgggtaa
     5881 aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca cgacttctgg
     5941 tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg aaccgcatga
     6001 cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag tgctgtaccc
     6061 ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg cacgctccat
     6121 taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag gttcgctgac
     6181 atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc aggtgccgga
     6241 tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga gtgatgacgg
     6301 tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg
     6361 tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag atatcgctgc
     6421 tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg accgcaaatt
     6481 gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata acctggcggc
     6541 agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt tgattcagca
     6601 aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt ggaatcactt
     6661 tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg catccgacac
     6721 tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga gatagcgaac
     6781 cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga tagattagct
     6841 ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca ccaccgacgg
     6901 acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg ccaccatccc
     6961 caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc
     7021 tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca tcgctacacc
     7081 aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt tcacaccatc
     7141 aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa
     7201 aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg gcgtcactgc
     7261 cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca tcagactaac
     7321 gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg tgtcgatcgc
     7381 cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg taacggcaat
     7441 caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc
     7501 atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg aacccagcgg
     7561 acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata attcaatcat
     7621 gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg atgattatga
     7681 tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga aataaactcc
     7741 ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt caggcacagc
     7801 acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa aacgccacaa
     7861 tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc atttacatcc
     7921 acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa tgctcgcgct
     7981 aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt aatcaggtgc
     8041 ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc agggcgtaag
     8101 cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt ctgcggtaca
     8161 tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt taccagcccg
     8221 agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa agtcaaaatt
     8281 tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc aaaagaaacc
     8341 gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca gtagtcatga
     8401 aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt atgttacata
     8461 aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca acacaggccg
     8521 cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct gccattgctg
     8581 atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat gattattcac
     8641 tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg gttggcccgg
     8701 agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag caggtaacga
     8761 ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc gatgatgatg
     8821 atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc aacatgcatc
     8881 tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga aaattagtgg
     8941 aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt gaggcacaat
     9001 tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg aaaggttatt
     9061 tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca ccgcttggtc
     9121 attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa ataagaacac
     9181 agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg ccagcggtcg
     9241 ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc ctggggacga
     9301 atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc aaccagtatg
     9361 cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata gcccgctctg
     9421 tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg
     9481 ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat gccacgccaa
     9541 ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct gaagccagtg
     9601 agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa ctggatgaca
     9661 aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat acgctaagca
     9721 gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct gctgccgaca
     9781 aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc gcggacggcg
     9841 aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg
     9901 ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt aacaatctgc
     9961 tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt ttaaccagcg
    10021 ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc aaactgaaaa
    10081 aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa
    10141 gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca atccgcgctg
    10201 aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc ttgcgattcc
    10261 cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact aatccggtga
    10321 caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt acgccagtgc
    10381 tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca ggttattatg
    10441 tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc aagattctgg
    10501 tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt aataccggac
    10561 gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc gtaaacccgc
    10621 tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc gaattcctgg
    10681 cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc attcgcagcc
    10741 ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc cgctacacta
    10801 tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa
    10861 tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact ggggagcatg
    10921 gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc gtgatcccat
    10981 tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt
    11041 aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag cgattgttga
    11101 tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg
    11161 gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg aagagatgcg
    11221 gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg atattcctgc
    11281 catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc agggcaaccc
    11341 ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag
    11401 aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg gatattattt
    11461 acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg ccacctataa
    11521 agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg ttgtaccgct
    11581 gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc caccgatgga
    11641 tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag aagtcgagat
    11701 ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga aaactcgcaa
    11761 accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat aaaaaagcct
    11821 ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa tattcgaaat
    11881 ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat ccggcaacgt
    11941 acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact catcacgtcg
    12001 ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg cagcagatta
    12061 accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac cggcgtaacg
    12121 ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc gaaaattggt
    12181 agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt catggtaatg
    12241 atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc aatagcttct
    12301 tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct cgggattttt
    12361 tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata ctgcccagcg
    12421 gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt tgctacgtcc
    12481 atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat ctgggtcgcc
    12541 gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg aatgccgacc
    12601 agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac cacgtcagag
    12661 gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg ttcaaccttg
    12721 gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata gttcagatct
    12781 tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc cgcagtctta
    12841 atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc aagtttgttg
    12901 atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac tttcatgccc
    12961 tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat gtcaccaggc
    13021 acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc gccttcacct
    13081 ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac tttgccttct
    13141 ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc cacatgacgc
    13201 cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc ttcaggcgag
    13261 ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt ttcaagatta
    13321 ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg aagccttttg
    13381 gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga aatccgaatg
    13441 aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag attaggacag
    13501 tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa aacgcgattc
    13561 cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc gtcgcaaagt
    13621 aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa ccatgggcat
    13681 ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt cccgggcgag
    13741 cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg ccagctgcgc
    13801 cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca cgtctccgtc
    13861 gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca ccaccggaac
    13921 attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag agcctaatcc
    13981 gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga tggcagattt
    14041 atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata ttttccccgt
    14101 gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag tgccattcgc
    14161 cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca tgctgcgaca
    14221 gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag caatactcga
    14281 atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct ctgatttgct
    14341 caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa gagactcacg
    14401 ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga tattacgcct
    14461 gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca taacatgatc
    14521 agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg ccgcgctttg
    14581 cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca agctcgtaaa
    14641 agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta ccgggttagt
    14701 taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga cctggtcatt
    14761 ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta tcaactggaa
    14821 aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc tgactgggat
    14881 aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa agaaaccatt
    14941 gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct cgatgtcaat
    15001 gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg tatcactatt
    15061 aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct tggcgaggca
    15121 aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac gtcgctggcg
    15181 acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca ggtttaccgt
    15241 atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg ttttgctaac
    15301 tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat taccgtggca
    15361 gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca gatgcgtgac
    15421 atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc gccatctgac
    15481 ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct gcgccgcatc
    15541 gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg cttcgcccag
    15601 ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag caatacagaa
    15661 accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt gccattctac
    15721 ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta tttcaaaaca
    15781 cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa actgactatc
    15841 cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc tggccttgac
    15901 cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac ctttaatcag
    15961 acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg tattcaggca
    16021 ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc cattactgag
    16081 gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg gggacccgtt
    16141 gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga gtaatatcag
    16201 cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg tagaacaatt
    16261 tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta cactgactga
    16321 aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc tctgaatgaa
    16381 acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat gaatccacaa
    16441 ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc tgcttatctc
    16501 gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc atgcggtaac
    16561 ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa aagcatgttg
    16621 cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca ccagccttat
    16681 gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt tggtcaggtt
    16741 gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg aatggaattg
    16801 tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc ccataacatg
    16861 tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct gacgatggca
    16921 gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat ggcaagcggt
    16981 ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa agtggaccgc
    17041 atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg tactttctac
    17101 ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt gccaggctct
    17161 tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc tgcgcgtcag
    17221 gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat gatcgatgag
    17281 aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac taaccacacc
    17341 atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga tgacttctct
    17401 gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc ggccgatatt
    17461 aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct caaagcaggc
    17521 ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta tacccttgaa
    17581 ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc actcgacagc
    17641 aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa agtgttaagc
    17701 ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa ccaggtgatt
    17761 gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt tgaagcgggt
    17821 ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc gaacggaatg
    17881 ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg tttcaaaatt
    17941 gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc agctatccac
    18001 gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg ggacatcatt
    18061 cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga actggctgct
    18121 cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg tgaattattc
    18181 agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat cactttttaa
    18241 gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa aacaagtgca
    18301 gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa actggaacac
    18361 gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga agtgactctg
    18421 cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc tgaagcgatt
    18481 gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga agcgggtgca
    18541 cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac cgaagggact
    18601 attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat ggactacggt
    18661 ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc cctgcaggcg
    18721 atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc tccggctaac
    18781 taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg gctggttccg
    18841 gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg tgaagctgta
    18901 gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt tttgtagcgt
    18961 ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc gcgcgttcaa
    19021 tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg ctgccatcaa
    19081 tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc tgcacattat
    19141 caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct gcaggaccat
    19201 actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg gcaaactctg
    19261 agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg gagacctcac
    19321 tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca taaccgccca
    19381 gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc gggctcattt
    19441 gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct actggtgcac
    19501 agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag tcaaacttaa
    19561 cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga gcgtattccc
    19621 atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg gaagagctca
    19681 tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg aaaaggcttt
    19741 tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc gccaggcgac
    19801 ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca acattcagtc
    19861 cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc ggcacgatat
    19921 aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc atattaatga
    19981 catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg acggcaatca
    20041 cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca ccggagccta
    20101 ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc ataacgaata
    20161 tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc tttattgctg
    20221 atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat tatgagaggc
    20281 tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag aaaatgtcgt
    20341 ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg gatctgcttg
    20401 acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa gccctttcgc
    20461 tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc aaattaccgt
    20521 ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg gcctgaagca
    20581 acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct atggagaaaa
    20641 acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc gcatcagttt
    20701 tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa ggtctggatg
    20761 ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg gcgcgctggg
    20821 cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt ttgcaggaca
    20881 ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa agtgccgata
    20941 ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag cggcgttaat
    21001 ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg tccgtcccag
    21061 aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc aatcagcatc
    21121 aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat cacctgatat
    21181 acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc cccgcttatt
    21241 tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg ctacaggcac
    21301 taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc aatccctgct
    21361 tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca aactgttagt
    21421 gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag cacgtaaact
    21481 tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag cgggtacggt
    21541 gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac cgcagtttgg
    21601 cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc tggtctttgc
    21661 cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc agcaattgcg
    21721 tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg aacaaccgct
    21781 ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg aaatctattt
    21841 cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc tgaatgcact
    21901 gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg atctcgaaca
    21961 gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt tgactatttc
    22021 gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct gcataacatt
    22081 cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt cgcttgcatc
    22141 cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa taacatgcta
    22201 ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg catcgataat
    22261 tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta cctgcaacaa
    22321 gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga tcgcatctta
    22381 aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat aaaaaatggc
    22441 taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca gcgtcaatcg
    22501 gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa gcgcgcagct
    22561 catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac cattatggcg
    22621 ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa tctggaaaca
    22681 ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg ggcaaatgac
    22741 tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta tcaggtctgg
    22801 cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga aaaagacgat
    22861 acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat tcatctggcc
    22921 agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc cgagccgttt
    22981 gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca gcatcgtttt
    23041 tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg tatgcgtgat
    23101 gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga agggtttacg
    23161 ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa
    23221 attaaccgca agacccggga agtcattggc attgcctttg atgatccggc ctatgtgacc
    23281 tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta ttcttccatg
    23341 accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg tgtattaaaa
    23401 caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct gtggatagtc
    23461 gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca ttttcgcaaa
    23521 ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat tgatgccgat
    23581 ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat tgtccatgtt
    23641 cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct gaagaagaaa
    23701 aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg ctatggctct
    23761 ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg cgttgcaatt
    23821 aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt tgatgttgta
    23881 acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga gtggggaaat
    23941 ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga caacgtcacc
    24001 gcacaggctt atccgcattt actggtaacg accggtttac acgattctca ggtgcaatat
    24061 tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga ccatctttta
    24121 ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt taaatcgtac
    24181 gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct
    24241 acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc
    24301 atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc ggcaacgtcg
    24361 gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga cggacgtccg
    24421 gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac
    24481 gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag cggtgtctgt
    24541 acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc tggccacaaa
    24601 cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg
    24661 tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct
    24721 tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt
    24781 ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat ttttccgtca
    24841 atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg
    24901 ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga atgatatcgc
    24961 cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa
    25021 cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt gccatcagca
    25081 cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg
    25141 gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata tccatacctt
    25201 gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac
    25261 tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa
    25321 agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag gtcgttgcgg
    25381 cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca cgtcgcgaat
    25441 aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg
    25501 cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct
    25561 catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc
    25621 aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt
    25681 aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gattgaccga
    25741 agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt ctggctgttc
    25801 acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag ccaccccggc
    25861 cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt
    25921 cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gcctggatgg
    25981 caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag
    26041 ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg
    26101 gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat
    26161 cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac tttaaacttc
    26221 tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa aaacgaaaat
    26281 attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat tgttccactg
    26341 gccgattcgc tgaaacccgg aaccaacacc gttgactggc atgttgtttc ggtggacggg
    26401 cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc gtttacctgg
    26461 atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt tgcaatgtat
    26521 ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc tctgcgttta
    26581 caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc cgttcagggc
    26641 gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca ggctgttttg
    26701 caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt aacgttgatc
    26761 gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa
    26821 tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac agcgaaaatc
    26881 catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg gggattactg
    26941 ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc tattcaggcg
    27001 ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg
    27061 cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg gggccaactc
    27121 ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc taatcgttat
    27181 gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg
    27241 actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct gcttgcgact
    27301 ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta
    27361 ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag tcgttttgaa
    27421 gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg ccgaccgggt
    27481 aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa cgatatcgca
    27541 caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat tcagattgat
    27601 gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa
    27661 ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc tcagttcttt
    27721 caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt tattgaccat
    27781 tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca aggcaactaa
    27841 gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt tccctggacc
    27901 gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt agttgcaaac
    27961 gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt ttttggttat
    28021 atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag taagtacgtt
    28081 ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc aagtagacgc
    28141 tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct tctgatttcg
    28201 tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg ctgcgacaaa
    28261 tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc taatcccggc
    28321 actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg caccaagtcg
    28381 taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc ctgtcttaat
    28441 ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg ttatgtcttc
    28501 gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg ccggaagttg
    28561 atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc ttgtaactac
    28621 tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt caacaatggc
    28681 ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt ttttccctcc
    28741 ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg tcagtaaaac
    28801 ccctcctaat ttttctttta ttgtctcca
//
LOCUS       DAJTYM010000047        28760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTYM010000047 DAJTYM010000000
VERSION     DAJTYM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..2126)
                     /gene="yccS"
                     /locus_tag="OO575_002093"
     CDS             complement(<1..2126)
                     /gene="yccS"
                     /locus_tag="OO575_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6665055.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQ"
     gene            complement(2136..2582)
                     /locus_tag="OO575_002094"
     CDS             complement(2136..2582)
                     /locus_tag="OO575_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6665056.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2705..4759
                     /gene="helD"
                     /locus_tag="OO575_002095"
     CDS             2705..4759
                     /gene="helD"
                     /locus_tag="OO575_002095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6665057.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4791..5249)
                     /gene="mgsA"
                     /locus_tag="OO575_002096"
     CDS             complement(4791..5249)
                     /gene="mgsA"
                     /locus_tag="OO575_002096"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6665058.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5345..6007)
                     /locus_tag="OO575_002097"
     CDS             complement(5345..6007)
                     /locus_tag="OO575_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6665059.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6180..6593
                     /locus_tag="OO575_002098"
     CDS             6180..6593
                     /locus_tag="OO575_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6665060.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6638..6955)
                     /gene="hspQ"
                     /locus_tag="OO575_002099"
     CDS             complement(6638..6955)
                     /gene="hspQ"
                     /locus_tag="OO575_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6665061.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7013..8203)
                     /gene="rlmI"
                     /locus_tag="OO575_002100"
     CDS             complement(7013..8203)
                     /gene="rlmI"
                     /locus_tag="OO575_002100"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6665062.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8298..8576
                     /gene="yccX"
                     /locus_tag="OO575_002101"
     CDS             8298..8576
                     /gene="yccX"
                     /locus_tag="OO575_002101"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6665063.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8573..8902)
                     /gene="tusE"
                     /locus_tag="OO575_002102"
     CDS             complement(8573..8902)
                     /gene="tusE"
                     /locus_tag="OO575_002102"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6665064.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8993..9652)
                     /gene="yccA"
                     /locus_tag="OO575_002103"
     CDS             complement(8993..9652)
                     /gene="yccA"
                     /locus_tag="OO575_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6665065.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9859..9946)
                     /locus_tag="OO575_002104"
     tRNA            complement(9859..9946)
                     /locus_tag="OO575_002104"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:tga)
     gene            10372..11490
                     /gene="hyaA"
                     /locus_tag="OO575_002105"
     CDS             10372..11490
                     /gene="hyaA"
                     /locus_tag="OO575_002105"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6665066.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11487..13280
                     /gene="hyaB"
                     /locus_tag="OO575_002106"
     CDS             11487..13280
                     /gene="hyaB"
                     /locus_tag="OO575_002106"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6665067.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13299..14006
                     /gene="hyaC"
                     /locus_tag="OO575_002107"
     CDS             13299..14006
                     /gene="hyaC"
                     /locus_tag="OO575_002107"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6665068.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14003..14590
                     /locus_tag="OO575_002108"
     CDS             14003..14590
                     /locus_tag="OO575_002108"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6665069.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14587..14985
                     /gene="hyaE"
                     /locus_tag="OO575_002109"
     CDS             14587..14985
                     /gene="hyaE"
                     /locus_tag="OO575_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6665070.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14982..15839
                     /locus_tag="OO575_002110"
     CDS             14982..15839
                     /locus_tag="OO575_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6665071.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15973..17517
                     /gene="appC"
                     /locus_tag="OO575_002111"
     CDS             15973..17517
                     /gene="appC"
                     /locus_tag="OO575_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6665072.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17529..18665
                     /gene="appB"
                     /locus_tag="OO575_002112"
     CDS             17529..18665
                     /gene="appB"
                     /locus_tag="OO575_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6665073.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18677..18769
                     /gene="cbdX"
                     /locus_tag="OO575_002113"
     CDS             18677..18769
                     /gene="cbdX"
                     /locus_tag="OO575_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6665074.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18849..20147
                     /gene="appA"
                     /locus_tag="OO575_002114"
     CDS             18849..20147
                     /gene="appA"
                     /locus_tag="OO575_002114"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6665075.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20262..22428)
                     /gene="etk"
                     /locus_tag="OO575_002115"
                     /pseudo
     CDS             complement(20262..22428)
                     /gene="etk"
                     /locus_tag="OO575_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22448..22894)
                     /gene="etp"
                     /locus_tag="OO575_002116"
                     /gene_synonym="yccY"
     CDS             complement(22448..22894)
                     /gene="etp"
                     /locus_tag="OO575_002116"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6665076.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22882..24021)
                     /locus_tag="OO575_002117"
     CDS             complement(22882..24021)
                     /locus_tag="OO575_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6665077.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24067..26163)
                     /locus_tag="OO575_002118"
     CDS             complement(24067..26163)
                     /locus_tag="OO575_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6665078.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26163..26909)
                     /locus_tag="OO575_002119"
     CDS             complement(26163..26909)
                     /locus_tag="OO575_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6665079.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26906..27550)
                     /locus_tag="OO575_002120"
     CDS             complement(26906..27550)
                     /locus_tag="OO575_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6665080.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27657..27962)
                     /locus_tag="OO575_002121"
     CDS             complement(27657..27962)
                     /locus_tag="OO575_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665081.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28404..28616)
                     /gene="cspH"
                     /locus_tag="OO575_002122"
     CDS             complement(28404..28616)
                     /gene="cspH"
                     /locus_tag="OO575_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6665082.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         6953 a         7288 c         7574 g         6945 t
ORIGIN      
        1 atctggcgtt gcagacgacc aatctctggc agtaatgcaa tcaataaccc aacttgttgt
       61 acgaccagag gttctttgct atctgcccgt ggttcaagtt gctgcatccg ctgtttcagg
      121 ccagctaatg cctgattgac gcgttcttca tcagcaggtt gatgatgtaa cgcgtcatca
      181 acatagcaaa ctgcgtcatc aagaaacgcc agaatttcag gattagttaa ctgctcccgg
      241 tgagcaccga gggctgagat atagctggta aacgtatggt taaggcacag caagcgaaac
      301 gcggcttcgc gaatttgcgg ggtaacgttc ggttcgctgg acatatttga taccaccgac
      361 gccagttcag catcacggtt gtgtgcatcg cggcgggcaa tacgatacgc cagacggtta
      421 tcacgcccct gatggtattg ctccagtatg gcatcgagat agcgacagtt ggcctctgtg
      481 gcgcgttcga gcattcgcgg cagattgcga aacttccagt caggccagat gtagctcact
      541 gccgcccacg caatggcaca accaatcagc gtatcgatta cgcgaggtaa cgctacttca
      601 aaaccttcac ccagtaagtt aaaacacagt agcaccaaaa gtgtgatgaa catcgttgca
      661 tgagcgtatt gcacgttacg gaaggcaaaa aagagcacgc cggtaataac cagcagcacc
      721 agctgccctt ccagtgatgg cacaaaccac agcacaggaa tgccaatcgc gatacctacc
      781 agcgtaccaa taatccttaa cttcaggcgg tggcgcgtgg cgttatagtt tggctggcag
      841 acaaacaaac ttgtcagcaa gatccaatac ccgtgatgca ttccggttat ttgaatgatg
      901 gcgtagccga agcacaacac cagcgacatt cttaccgcat gacggaagag ggcggattcc
      961 ggcgtgaagt gacggctaag acgcagccag atatcactca atccgtgcgg gctgtcatca
     1021 gcgagctcat tttcgtcatt attacggggt agtgtctggg cctgttctga ttcaattgtt
     1081 gccagttggg catcaatggc gcgtaaattg ttcagcaaaa atcccagtgt tttgagtaaa
     1141 tcggcgggtg cgccgttatc gcgtatccgc tccagcgcag catcaatatg cgtaaaagcg
     1201 cgctcaaaat gcggatcatg ttgataagac tgacgcaaca aaatacagcg tgacagttgc
     1261 tggcacgcct ggccctgcat cgacatcagc cgctgaaaac ggaacagcac gtcgctgtgg
     1321 cgaaaatgtt cacgcaatgt ttgatactga atatgagaag agctggcacg ctcgtgaata
     1381 tcctgtgcga caaagtaata atgcagcgtg cgacgcgttc cccgttgacc acgatcgcca
     1441 cgtaagcggg tcagcagcga gagtttcgtc tgattcaatg tcgccatcag ctgaccgttg
     1501 gcgagagcca aatcgtacag cggtgcctgg ctttcatctt caatatcagg atcaaacatg
     1561 cgcgacttga gctcaagata acgcgccagt tgttcatagc aacgcgccag gttgtcctgc
     1621 agcgggcgga ccgggaacag cagatgacca ataagtgtca ggacgttgta ccagacggca
     1681 ccggccagca gatacatcgg ctgctgatac cagtgctcat acagtgatgt tcccaacata
     1741 gtgtaaatgg cgatcagcaa tgcaccgaag gcaattgttg catagcgttg acccagaccg
     1801 ccgagcaaaa tgaagccgct ggtagagagc gttaagccaa tcgcaaatag ccagggccag
     1861 ggaaacagca attctactga ggccgaggcg ataaaaaagc agaacagcgt aatgatgagg
     1921 ttacgcaaac gtcccgccag tcggtcatcg agatcggtca gcgccgctgc caccatcccc
     1981 agcgttagcg gaatcgtcag ttttacatca cccagccacc acggaaacgc tgtggttcca
     2041 caaagcgcaa taaaaatacg cgcgtaatac agccaggcgc tgttccaggt atagcgtttg
     2101 agcaaaggac ttagcataaa ggccatatta gtggattatt caaaacgacg acgtgcattg
     2161 gcttcacgcg cagcttgcgc tgtttctacc gataccacgc gacgaccaac cggccatagt
     2221 gcaatagcgg caattttaaa gttcgcaatg ccgacaggaa tgccaatgat tgaaatacat
     2281 tgtgcgatgc ccgttgcaat gtgcatcagg cataaccacc agccaaagaa aatcagccag
     2341 aaaatattca ataccgtacc gccagtattc agcaacacat ttttgccagc cgggttcagt
     2401 tcatcgacat gaatagcttc attgccataa ggcaccagag acagtttagt gatctcccag
     2461 caggatcgtg tcagcggtaa ggtaaaaatc agcacaatac tgaccagagt cgccaacagc
     2521 cagcccagag tggtggcaaa tccgccaagc acaaagttca gaatgttcaa aacggtacgc
     2581 ataaaacctc gctttactgt ggttttcagt aatgggcggc aattgtaacg tttttttggg
     2641 ctggagcacg ttttctctga cggttacact gataagaaat aatttcgtgt ggatctacag
     2701 agtcatggaa ctgaaagcga caacgcttgg aaaacgtctg gcacagcacc cttacgatcg
     2761 ggcggtgatc ctcaatgccg ggattaaagt ctccggcgat cgccacgaat accttattcc
     2821 tttcaatcaa ttactggcga ttcactgtaa gcgcggtctg gtatggggcg agctggaatt
     2881 tgtactgccg gacgaaaaag tggtgcgtct gcacggcaca gaatggggcg agacgcagcg
     2941 tttttaccat catcttgatg ctcactggcg gcggtggagt ggcgagatga gcgaaattgc
     3001 gtctggtgtt ttacgccagc aactggattt gattgccacg cgcactggag aaaataaatg
     3061 gctgacgcgt gagcaaacct ctggcgttca gcaacaaatc cgccaggctt tgtcggcgtt
     3121 gccgttgccg gttaaccgac tggaagaatt cgataactgc cgtgaggcgt ggcgtaaatg
     3181 tcaggcttgg ttgaaagata ttgaaagcgc tcggttgcag cataaccagg cgtataccga
     3241 agccatgctt accgagtatg cggatttttt ccgccaggtc gagtcttcac cgctgaatcc
     3301 ggcgcaggcc cgggcagtcg ttaatggcga gcattctctg ttagtgctgg ctggtgcagg
     3361 aagcggaaaa acgtcggtgc tggtggcccg tgcaggctgg ttgctggcgc gtggtgaagc
     3421 gtcccctgag caaattttat tgctggcgtt tggtcgcaaa gccgctgaag agatggacga
     3481 gcggattcgc gaacggctac ataccgaaga cattaccgca cgcacgtttc atgcgttggc
     3541 gctgcatatt attcagcagg gcagcaaaaa agttccgata gtcagcaaac tggaaaatga
     3601 taccgctgcc cgtcatgaac tctttattgc tgagtggcgc aagcaatgca gcgaaaagaa
     3661 agcgcaggcg aagggctggc ggcaatggct gacggaagaa atgcagtggt cagtgccaga
     3721 aggtaacttc tgggatgatg aaaaattaca gcgtcgcctt gcctctcgcc tcgatcgctg
     3781 ggtaagtctg atgcggatgc acggtggtgc acaggcagaa atgattgcca gtgcacccga
     3841 agagattcgc gatctgttca gtaaacgtat caagttgatg gccccgttat taaaagcctg
     3901 gaaaggtgcg ctgaaggcag aaaacgctgt cgatttttcg ggccttattc atcaggcgat
     3961 tgtgattctg gagaaaggcc gctttatcag cccgtggaag catattctgg ttgatgaatt
     4021 tcaggatatc tcgccgcagc gcgcagcgtt gttagcggca ttacgcaagc aaaacagtca
     4081 gacgacgttg ttcgccgttg gtgatgactg gcaggcgatt taccgattca gcggtgcgca
     4141 aatgtcgctc accaccgctt tccatgaaaa ctttggtgaa ggcgatcgct gtgatttaga
     4201 cacgacttac cgttttaaca gtcgtatcgg tgaggtggca aaccggttta ttcagcagaa
     4261 cccaggccag ctgaaaaagc cgctaaacag cttaaccaat ggagacaaaa aagccgtcac
     4321 gttattggat gagagtcaac ttgacgcttt gctggataag ctctctggtt atgccaaacc
     4381 ggaagagcgc attctgatcc tggcgcgtta ccatcacatg aggcctgcca gcctggaaaa
     4441 agcggcaaca cgctggccga agttgcaaat cgactttatg accattcatg ccagcaaagg
     4501 gcaacaggcg gattacgtca tcatcgttgg cttgcaggag gggagtggtg gttttccggc
     4561 tgcggcgcgg gagtcgatta tggaagaggc gctactgcca ccggttgagg atttcccgga
     4621 cgctgaagaa cggcgtttaa tgtacgtggc gctgacccgg gcacgccatc gggtatgggc
     4681 actgtttaac aaagagaatc cctctccctt tgtggaaata ctgaaaaatc tggatgtgcc
     4741 ggtggcgaga aaaccgtaag aaacaggtgg cgtttgccac ctgtgcaata ttacttcaga
     4801 cggtcagcga gataacgctg ataatcgggg ataagaatat cgaccgcgtc gttgaaatgc
     4861 ggcgactgga taatgaagtc tgccgttgcc acgttggtgg cgaccggaat gttccatacc
     4921 gtcgccagac gcagcaaggc tttcacgtca ggatcgtgcg gcacggcatt tagtggatcc
     4981 cagaagaata tcaatacatc aattttccct tctgagatca atgcgccaac ctgctggtca
     5041 ccccccattg ggccactcaa catcgcgttg acgttcatgc cggtcgcgcg ggaaatcaag
     5101 ttaccggtag tgcctgttgc atacagtacg tgttgttcca gtaacggttg atgccgttcc
     5161 acccagctca tcagcatttg tttgcagtga tcgtgtgcca ccagcgcaat atgtttccgc
     5221 gcaggtaaag tgcgagtcgt cagttccata atgtacatcc gtagttaact ttcctacaga
     5281 ttactgtaag cacttatcgc tgcaagataa agaccgaaaa agcctgcgca caggcacaaa
     5341 aatctcagga agatggttgt ttttccgccc attgcaggaa agtatttcgc gtttgtgggt
     5401 cagccagttt aaaccaatac ttcagccgtt gttctgtgag cacctgagac tgcggtggaa
     5461 tagcatccag ctcggaaaca cccgaaagca gcgtactgtc atctgccatc atcgtggcaa
     5521 attgcggtag tgaagcgcgt ttcgcggatt tgttatagcg ttcaacctcg acctcataat
     5581 caatcgtttt tgccgttgag gtaatggcga gaatatccag ctttaccgga atcggcgtcg
     5641 catcgccatc caacaattca aggcgcggcg cggcatcaaa atggttagct tcccgctcat
     5701 tctccaggcg aggcaggcga aaattgacct ggttgatgag ctgggtatta aaactgacca
     5761 ccagcggtgg ggagatatac agccgttctt cactattgga aagatgaatc gtcttctcaa
     5821 cgcgaaacac taactgatgc ggtccattgt ccagttcaat gctgtccgca ccgcgcagca
     5881 gagagctgga aaccttcttg ccgtcgagta ccagaagatc gacatcggtt gaaagccgca
     5941 gcgtggtggc aaaaacggat accggcaaac ataatgcaat caaggtggtc acgatgccgg
     6001 ttttcatagg tagctcctgt caaaagaccg cccgaataat aatgtgtcaa aatttttctt
     6061 taatcatact tactaacata tagacatatt tcccgctttt gctctcattc attcgtatta
     6121 gctgcatggt tggcatgtcg gctttgtcgt acactttgtt aaacagtctg gaggaaataa
     6181 tgaaagaaac cgatattgca ggcattttaa cgtccactca caccattgcg ctggtggggg
     6241 caagcgacaa acccgatcgc cccagctatc gcgtgatgaa atatctactc gaccaggggt
     6301 atcacgtcat tccggtttcg ccaaaagttg ccggcaaaac gctgctgggc cagcaggggt
     6361 atggcacgct ggcagacgtt ccggaaaagg tcgatatggt cgatgttttc cgtaattcag
     6421 aagcggcgtg gggcgtggca caggaagcta ttgccattgg cgcgaaaacc ctctggatgc
     6481 aactgggggt gattaatgag caagcggcag tactggcacg ggatgccggg ttaaatgtgg
     6541 tgatggatcg ctgcccggct attgagatcc ctcgcctggg tctggccaaa taaaaaatcc
     6601 ccggaaggca aaaaccttcc ggggatttgt tcagggatta gttacgcaga cgcggggcct
     6661 ggagttgttt gcggatggtc tgcgccagct catccatgga tggctgttca ggatgttcat
     6721 cctgcaattc gctacttagc tgggcttcag ccaggtaggt atgaaccggt aggccgttat
     6781 cgtcctccat caccacgtga taccacggag cggcgcgaag ctcgtcattc accgccagct
     6841 catcgggcga cggttcagag agcgaataaa ccgggtcgat atccacgacc actccgagat
     6901 aacctaacag ggaatggcgg acctgctggc cgataccgaa tttgctggca atcatagtca
     6961 cctcccggga aatctttacc tttgatataa gggtaaaatt ccacatttca agttacatga
     7021 cgcgacaggc aaaccctttc agatatagcc cttccgggta ggtagcgatc accggatgat
     7081 cggctgcctg acggaactgc tctataaatt gtacatcacg accggcatca attgcggcat
     7141 ccgcgatgat tttctgaaat aaatcgctgg tcatcagacc ggaacaggag aaagtcagga
     7201 gaataccgcc ttcattcagc aactgaatcg ccagcatgtt gatgtcttta taaccacgac
     7261 acgcgcccat caactggctt ttattctcaa caaacttcgg cgggtccatc acgataacgt
     7321 caaatttttc accgcgatcg cgataagtac gcagcaattt aaagacatca tcacggacaa
     7381 actcagcctt gctcagatcc agtttgttca gctcaacgtt ctgccgtgca atatccagcg
     7441 cttcctggga ggtatcaacg ctgacaacct ggctgcaacc gcccatcagt gccgatacgg
     7501 cgaaaccacc ggtataggag aaacagttca gcacacgttt attttcaacg tagcggcggg
     7561 tagccaggcg gctgtctcgc tggtccagat agtagcccgt tttgtgtccg tgctgaatat
     7621 ccaccagcag cttcatgccg tgttcttcaa tcggcagcag ggcaggcggc aactcgccgg
     7681 tgacgaggcc ctgggtcagc tccatccctt cttttttacg taccgcaaca tcgctgcgat
     7741 cgtaaatcgc acattccggg tacagcgttt gcagggcact aattaatgcc gcgcgctgat
     7801 attctgcgcc agcactcagc agttgcagca ccagaaaatt accgaaacga tcgatagtaa
     7861 tacccggcag gccatcagat tctccggcga ttaaacgata gctgtcgagg ccatcttttt
     7921 gcgccagcca gtcacgccat ttttgtgctt gttgcaaacg gcgggaaaaa aaagcaatgt
     7981 cgatagactc agacgggtca aacgtccaga cgcgcgcccg gatttgcgaa gctggcgaat
     8041 aagcgccgcg tgctaaccat tttccctgat gatcaacaat atcgatggtt tcaccgaggc
     8101 tggctttacc ttccatgcgg gcaacggccc cggaaaagac ccacggatgg cgacgaagta
     8161 atgatttttc gcgccctttg gctaacacta aacgtacact cataatttac ttttctgtcg
     8221 atgcaaagaa atgggcgtca ttgtccggag ttttccatgg atttgcaacg cgtccattaa
     8281 ggataaggag agcagcaatg tcgaaaatct gcataattgc ctggatttac gggcgagttc
     8341 agggcgtagg atttcgctac accacacagt acgaagcgaa aagactgggc ttaaccgggt
     8401 acgccaaaaa tcttgacgat ggcagcgtgg aagtggttgc ctgcggtgaa gaagggcagg
     8461 tagaaaaatt aatgcagtgg ttaaagagtg gcggcccgcg ttctgcgcgt gtagaacggg
     8521 tgctcagcga gccgcatcat ccctcggggg aattaaccga ttttcgtatc cgttaaatac
     8581 attttaccgg ttttggcagg ccagcaattt tggtggcttg cttagccggg cctttcggga
     8641 acagtcggta taaatagcgg ctgttacctt tttcttcacc aaatttattc gccatcgctt
     8701 ttaccagcat acgaatcgcc ggagacgtat taaattccag gtagaagtca cgtacaaaac
     8761 gcaccacttc ccagtgttct ggcgacagcg aaatcccttc gttctctgca atcaccactg
     8821 ccagcggttc actccactgg ctgctttctt tgagatagcc ttcggtatcc gtttctatct
     8881 ctttaccttc gaagatcagc ataattatta ctaccttaat cagactggcg gcagtgtaac
     8941 aaaaaacaaa gccccgcata aagcgaggct atgaaagtgt tagcgggtga gattaatcgc
     9001 ggctggcgaa gcccagaatg ctcagtaggc tgacgaagat gttgtacagc gaaacataca
     9061 ggctaaccgt ggcacgaata tagttcgttt caccgccatg aatgatgttg ctggtttcaa
     9121 acaagatagc gccagaggag atcagaatga agaccgcgct gatcgccaga tgcagagcag
     9181 gcagttgcag gaagatatta gcaatcatac caatcagtac caccacaata cccgccatca
     9241 gcataccgcc gaggaacgac atatctttgc gggtggtcag cacatatgca gagcagcaga
     9301 agaacactaa cgccgttccg cccagtgcca tagcgattac gtcacccatt ccggcagaca
     9361 gataggtgtt cagaattggt ccgaggatat aacccagaaa accggtaaag gcgaatgcgg
     9421 agataatccc ggtcggctta ttcgccgttt tataggtcag gaacatcaaa ccatacatac
     9481 ccaccagagt cagaatcaga cccggagatg gcagcatcag caccgtactg gcagttgcgg
     9541 taatcgccga aaaggccagc gtcaggctca gcagaaaata ggtattacgc agcaccttat
     9601 gggtgctaag cagtgatgta cggtcatgtg aagaactaac aatacgatcc attgagtcac
     9661 tctctatgac agatgtaatt aattaagcag cataatgata atgcgtaagg gcacccagaa
     9721 gttttaccca tctttacgca tttgatctgg aacaggttta acagcggatt atcaggtcat
     9781 taagcaaata taacgccctg agaattccga caggcaaaag aaaaaggggg tagcgtttag
     9841 ctaacccctt atcttatttg gcggaagcgc agagattcga actctggaac cctttcgggt
     9901 cgccggtttt caagaccggt gccttcaacc gctcggccac acttccggaa tgacgcgcac
     9961 tataaacatc ccgatgcggc gtgtaaaccc ctaatttgtt tgtttgcctg aaaaacagcc
    10021 aaaagtgcat tgatagcgtg aaataacagc agattgatca tttcatcacc atgaattcct
    10081 tctcttttac tcgtttagca accggctaaa catccccatc gcccggctaa agaaaaatag
    10141 gtccattttt atcgctaaaa gataaatcca cacagtttgt attgttttgt gcaaaagttt
    10201 cactacgctt tattaacaat actttctggc gacgtgtgcc agtgcagaag gatgagcttt
    10261 cgttttcagc atctcacgtg aagcgatggt ttgccttgct acagggacgt cgcttgccga
    10321 ccataagcgc ccggtgtcct gccggtgttg ctaggagaag agacgtgcga tatgaataac
    10381 gaggaaacat tttaccaggc catgcggcgt cagggcgtta cccggcgcag ctttctcaaa
    10441 tattgtagtc tggctgccac gtcgctggga ttaggcgcgg gaatggcacc aaagattgcc
    10501 tgggcgctgg agaacaaacc gcgcattccg gtggtatgga tccacggtct ggaatgtacc
    10561 tgctgtaccg aatcttttat ccgctccgct cacccactgg cgaaggacgt catcctttcc
    10621 ctgatttccc tcgattacga cgataccctg atggctgccg ccggaaccca ggcggaagaa
    10681 gtctttgaag acatcatcac gcaatataat ggcaaatata tcctcgcagt agaaggtaat
    10741 ccgccgctgg gcgagcaggg gatgttctgt atcagcagcg gtcgaccgtt tattgagaaa
    10801 ctcaaacgtg ccgctgccgg agccagcgcg attatcgcct ggggaacctg cgcgtcctgg
    10861 ggctgcgtgc aggccgcgcg acctaatccg acacaggcaa cgcctatcga caaagtcatc
    10921 accgacaaac ccattatcaa agtacctggc tgcccgccga tcccggatgt gatgagcgcc
    10981 atcattactt acatggtgac ctttgatcgc ttgccagatg tcgacagaat gggtcgtcca
    11041 ctgatgtttt atggtcagcg tatccacgat aaatgctatc gccgcgctca cttcgacgcc
    11101 ggagagttcg tccagagttg ggatgatgac gctgcccgca aaggttattg cctgtacaaa
    11161 atgggctgca aagggcctac cacctataac gcctgttcct ccacacgctg gaatgatggc
    11221 gtttctttcc caatccagtc tggtcacggc tgcctgggct gtgcggaaaa tggtttctgg
    11281 gatcgcggtt cgttctacag ccgcgtggtc gatattccgc aaatgggtac tcattccacc
    11341 gccgataccg tcggtttaac cgcgcttggc gtggtggcag cagctgttgg tgtgcacgca
    11401 gttgccagcg ccgttgacca acgcagacgt cataaccagc aacctacaga aaccgaacat
    11461 cagccaggca atgaggataa acaggcatga gcactcagta cgaaactcag ggatacacca
    11521 tcaataatgc cggacgccgc ctggtggtcg acccgattac gcgcatcgaa ggccacatgc
    11581 gctgtgaagt gaatattaac gatcagaatg tgatcaccaa tgccgtctcc tgcggcacca
    11641 tgtttcgcgg gctggagatc atcctgcaag ggcgcgaccc gcgcgatgcg tgggcgttcg
    11701 ttgaacgtat ctgcggcgtc tgtactggcg tacacgccct ggcttcggtt tacgccatcg
    11761 aagatgccat cggtattaaa gtgccggaca acgccaatat catccgcaac attatgctgg
    11821 caacgctctg gtgccacgat catctggtgc acttctatca gcttgccggg atggactgga
    11881 tcgacgtgtt agatgcgctg aaagccgacc cgcgaaaaac ctccgaactg gcgcaaagtc
    11941 tctcctcttg gccgaaatca tcccctggct atttcttcga cgtacaaaat cgcctgaaga
    12001 aattcgttga aggcgggcag ttggggatct tccgcaatgg ctactggggg cacccgcagt
    12061 acaaactgcc gccagaagcc aacctgatgg gctttgccca ctatctcgaa gctctcgatt
    12121 tccaacgtga aattgtcaaa attcacgcgg tctttggcgg taaaaacccg catccaaact
    12181 ggattgtcgg tgggatgcct tgcgccatta acattgacga aagcggcgcg gtcggggcgg
    12241 tcaatatgga acgcctgaac ctggtgcagt cgattatcac ccgcacggca gacttcatta
    12301 acaacgtgat gatccccgac gccttagcca tcggtcagtt caacaaaccg tggagcgaaa
    12361 tcggcacggg tctttctgat aaatgtgtcc tcagctacgg cgcattcccg gatattgcca
    12421 acgactttgg tgagaaaagt ctgctgatgc ctggcggcgc ggtgattaac ggcgacttca
    12481 acaatgtgct gccagtggat ttggttgatc cgcagcaggt gcaggagttt gtcgatcacg
    12541 cctggtatcg ttatcccaac gatcaggtcg ggcgtcatcc attcgatggc attaccgacc
    12601 cgtggtacaa ccccggcgat gtcaaaggca gcgataccaa cattcagcag ctgaatgaac
    12661 aggaacgcta ctcgtggatc aaagcgccac gctggcgcgg taacgcgatg gaagtggggc
    12721 cgctggcacg cacgttaatc gcttatcaca aaggcgatgc tgcgaccgtt gagtcggtcg
    12781 atcgcatgat gtcggcgttg aacctgccgc tttccggtat ccagtcaacg ttgggccgca
    12841 ttttgtgccg cgcgcacgaa gcgcagtggg ccgcaggtaa gttgcagtat ttcttcgaca
    12901 agctgatgac caacctgaaa aacggcaatc tcgccactgc ttccactgaa aaatgggaac
    12961 ctgcaacctg gccgacagag tgccgtggcg tcggctttac cgaagcgccg cgcggagcgt
    13021 taggccactg ggccgccatt cgcgatggca agattgatct ctaccagtgc gtggtgccga
    13081 ccacctggaa cgccagtccg cgcgatccca aagggcagat tggcgcttat gaagcggcgc
    13141 tgatgaacac caaaatggcg atccccgagc aaccgctgga gatactgcgt actctgcaca
    13201 gctttgatcc gtgcctcgcc tgttcaactc atgtgctggg cgacgacggt agcgagctga
    13261 tctccgtgca ggtgcgttaa cagcgaagga gaatcatcat gcaacagaaa agcgacaacg
    13321 ttgtcagcca ctatgtcttt gaagcaccag ttcgtatctg gcactggctg acggtgttat
    13381 gcatggcggt gttgatggtc accggttact ttatcggcaa gccgctacct tccgtcagtg
    13441 gcgaggcgac gtatctgttc tatatgggtt acatcaggtt aattcacttc agcgccggga
    13501 tgatttttac cgtggtcttg ctgatgcgga tctactgggc ttttgttggc aatcgttact
    13561 cccgcgagct gtttatcgtg ccggtatggc gtaaaagctg gtggcagggc gtgtggtatg
    13621 aaatccgctg gtatctgttt ctggcaaaac gtccgagtgc cgatataggc cataacccca
    13681 tcgcccaggc ggcgatgttc ggctatttcc tgatgtcggt ctttatgatc atcaccggtt
    13741 ttgcgctgta cagcgaacac agccagtacg ctatttttgc gccgttccgt tatgtggtgg
    13801 aatttttcta ctggacgggt ggcaactcaa tggacattca cagctggcat cggctgggga
    13861 tgtggctgat tggggcgttt gtgatcggtc atgtctacat ggcgctgcgt gaagacatca
    13921 tgtccgacga cacggtgatc tccaccatgg tcaacggcta ccgtagccac aaatttggca
    13981 aaataagtaa caaggagcgt tcatgagcga gcaacacgtg gtggtcatgg ggctgggcaa
    14041 cctgctgtgg gccgatgaag gcttcggcgt gcgggtggcg gaacggctgt atgcccatta
    14101 ccactggccc gagtatgtgg agattgtcga tggcggtact cagggactga acttgctggg
    14161 gtatgtcgaa agcgccagcc atctgttgat tctcgatgcc attgactacg ggctggaacc
    14221 tggaacgctg cgaacctatg ccggagaacg cattccggcc tatctcagcg cgaagaaaat
    14281 gagcctgcat cagaacagtt tctccgaagt gctggcgctg gcagatatcc gcggacatct
    14341 gccagcacat attgccctcg tcgggctgca acccgcgatg ctcgacgact acggcggcag
    14401 cctgagcgaa ctggcacggg agcaactgcc cgctgcggaa caatcggcgc tggcgcagtt
    14461 ggctgcgtgg ggaagtgtgc cgcaaccggc taatgaatcg cgttgtctca attatgactg
    14521 tctgtcgatg gaaaattacg aaggcgttcg cttgcgccag taccggatga cacaggagga
    14581 gcagggatga gcaacgacac gccatttgat gcgttgtggc aacgaatgct ggcgcgcggc
    14641 tggacgccgg tcagtgaatc ccgtcttgac gactggctta cgcaagcgcc agacggcgtg
    14701 gtgttattaa gcagtgaccc gaaacgcacg ccagaggtca gcgataatcc ggtaatgatt
    14761 ggcgaattac tgcacgagtt tcccgactat acatggcagg tggcgattgc tgaccttgag
    14821 cagagcgaag ccatcggcga tcgctttggc gcctttcgct ttcctgccac tttagtgttt
    14881 accggcggaa actatcgcgg cgtgctgaat ggtattcacc cgtgggcgga actgataaac
    14941 ctgatgcgcg ggcttgtcga accgcagcag gagcgtgcct catgagcgaa acttttttcc
    15001 atctgctggg gccaggaacg caaccgaacg atgacagttt cagcatgaat ccactgccga
    15061 tcacctgtca ggtgaatgat gaaccgagta tggcggcctt ggagcaatgt gctcacagcc
    15121 cgcaggtgat tgcgctgtta aacgagttac aacatcaact aagcgaacgc cagccgccgt
    15181 tgggcgaggt gctggcagtc gatctgttaa atctcaacgc cgacgatcgt cactttatca
    15241 atacgcttct cggggaaggg gaagtgtcag tgcgcattca gcaggctgac gacagtaaaa
    15301 gtgaaataca ggaggcgatc ttctgcggat tatggcgggt gcgcagacgt cgcggcgaaa
    15361 agttgctgga ggacaaactg gaggctggct gcgcgccgct ggcgttgtgg caggcggcaa
    15421 cgcaaaatct cttgccgaca gattcgctgt taccgccgcc cattgatggc ctgatgaatg
    15481 gcctaccgtt ggcgcatgag ttactggcac atgtacgtaa ccccgacgcg cagccgcaca
    15541 gcattaatct gacgcaatta cccatcagcg aggctgatcg gctttttctc tcgcgtctct
    15601 gtgggccggg aaatattcag attcgtacca ttggctatgg cgagagctat atcaacgcca
    15661 cggggttacg ccatgtctgg catttacgct gtacggacac cttaaaaggc ccgttactgg
    15721 aaagttatga aatctgccca ataccggaag tggtgctggc agcgccagaa gatttggtcg
    15781 actctgcgca gcggcttagc gaggtatgtc agtggctggc ggaagctgca ccgacgtaaa
    15841 aagacggtaa gtatcgcttt cagtcttatg aatatcgcaa tcggcgaata cctctggtcg
    15901 tagagtttca ggataaagag ggagatctaa cattatcggg ttatttttct ctcttcgcct
    15961 acaggagtgc gcatgtggga tgtcattgat ttatcgcgct ggcagtttgc tctgaccgcg
    16021 ctgtatcact ttttatttgt accccttacc ctggggctga tttttttgct ggctatcatg
    16081 gaaaccattt acgtggttac cggcaaaaca atctaccgcg atatgacgcg cttctggggt
    16141 aagctcttcg gtatcaattt tgctcttggc gtggctaccg gcctgaccat ggagtttcag
    16201 tttggtacta actggtcatt ctattccaac tatgtgggcg atatttttgg cgcaccactg
    16261 gcgatggaag cattaatggc cttcttcctc gaatccacct ttgtcgggct gttcttcttc
    16321 ggctggcaac ggctgaataa ataccagcac ctgctggtga cgtggctggt ggcgttcggt
    16381 tcaaatctct ctgcattgtg gatattgaat gccaacggtt ggatgcaata cccgaccggt
    16441 gcgcattttg atatcgacac cctgcgtatg gagatgacca gcttcagcga gctggtcttt
    16501 aacccggtca gccaggtgaa atttgtgcac accgtcatgg cgggctacgt gaccggggcc
    16561 atgtttatta tggcgatcag cgcctggtat ttactgcgcg gacgggagcg cgatgtcgca
    16621 ttacgctcgt ttgccatcgg ttccgtcttc ggtactctgg cgattatcgg taccctgcaa
    16681 ctcggagaca gttctgcgta tgaagtcgcg caagtacaac cggtaaaact ggcggcgatg
    16741 gaaggggagt ggcaaacgga acctgctcct gcaccgttcc atgtggttgc ctggccggaa
    16801 caggatcaag agcgtaacgc ctttgccatc aaaattcccg cgctgctagg gatcctcgcc
    16861 actcactcat tagataaacc cgtgccgggt ctgaagaatt tgatggctga aacctaccca
    16921 cgcttgcaac gcggacgtat ggcctggctg ttaatgcagg aaatatctca aggcaatcgt
    16981 gagccgcatg tgttgcaggc attccgggag ctggaaggcg accttggtta cggcatgttg
    17041 ctctcccgct atgcgccgga tatgaatcat gtcacagccg cacagtacca ggcggcgatg
    17101 cgtggcgcga tacctcaggt tgcgccggta ttctggagtt tccgcatcat ggtgggctgt
    17161 ggttccctgc tgctactggt gatgctgatt gcgcttgtcc agacgctgcg tggcaaaatc
    17221 gaccagcatc gctgggtgct gaaaatggcg ctctggagtt tgccgttgcc gtggattgcg
    17281 attgaagccg ggtggtttat gaccgagttt ggtcgtcagc cgtgggcgat acaggacatc
    17341 ttaccgacat actccgcgca ctccgcttta accacaggac aactggcttt ctcactgatc
    17401 atgatcgtag ggctttacac cctgttctta atcgccgaag tctacctgat gcagaaatat
    17461 gcccgtctgg ggccgagcgc gatgcagagt gaacaaccga cgcagcaaca ggggtaaagg
    17521 agaaaatcat gtttgattat gaaacattgc gcttcatctg gtggctgctg attggcgtga
    17581 tcctggtggt ctttatgatc tccgacggat ttgacatggg gatcggctgt ttgctgccgc
    17641 tggtggcgcg taatgatgat gaacgccgga tagtgattaa tagcgttggt gcgcactggg
    17701 aaggcaatca ggtctggttg atcctcgcag gtggggcatt atttgcagcc tggcccagag
    17761 tgtatgcagc ggcgttttcc ggcttttatg tggcgatgat cctggtgctg tgctcgctgt
    17821 tcttccgtcc gctggccttt gattatcgcg gaaaaattgc cgatgcacgc tggcgtaaaa
    17881 tgtgggatgc cggtctggtc atcggcagtc tggtgccgcc ggtagtcttc ggtatcgcct
    17941 tcggcaactt gttgctcggc gtgccgtttg ccttcacacc gcaattacgc gtggagtatc
    18001 tcggcagctt ctggcaactg ctgacgccat tccctttatt gtgtggtttg ctcagccttg
    18061 ggatggtgat tttgcaaggt ggcgtctggt tacaactgaa aactgttggt gtgattcatc
    18121 tgcgttcaca gctggcgact aaacgcgctg cactgttggt gatgctgtgc tttttgctgg
    18181 caggttactg gctgtgggtc ggtattgatg gctttgtact gctcgcccag gatgctaacg
    18241 gtccttccca tccgttaatg aaactggtgg cagtgctacc tggtgcctgg atgaataatt
    18301 tcgtcgaatc gcccgttttg tggatcttcc cgctgctggg gttcttctgc ccattgctga
    18361 cggtgatggc gatttatcgt ggtcgcccgg gttggggatt tttgatggca tcattgatgc
    18421 aatttggcgt gattttcacg gcaggcatca cgctgttccc ctttgtcatg ccgtcaagtg
    18481 tgagtccaat ctccagcctg acgttgtggg acagcacttc cagtcagctg acgctgagca
    18541 ttatgttggt aatcgtgctg atatttttgc ccattgtgtt gctctacact ctctggagct
    18601 actacaaaat gtggggacgc atgacaacag aaactctccg ccgtaacaaa aacgagttgt
    18661 actaaggagc aggaacatgt ggtatttact ttggttcgtc ggcattttgt tgatgtgttc
    18721 gctctccacc cttgtgttgg tatggctgga cccgcgtctg aaaagttaac gaacgtaggc
    18781 ctgatgcggc gcattagcat cgcatcaggc aatcaataat gttagatatg aaaagcggaa
    18841 acatatcgat gaaagcgatc ttaatcccat ttttatctct tctgattccg ttaaccccgc
    18901 aatctgcatt cgctcagagt gagccggagc tgaagctgga aagtatggtg attgtcagtc
    18961 gtcatggtgt gcgtgctcca accaaggcca cgcaactgat gcaggatgtc accccagacg
    19021 catggccaac ctggccggta aaactgggtt ggctgacacc gcgcggtggt gagctaatcg
    19081 cctatctcgg acattaccaa cgccagcgtc tggtagccga cggattgctg gcgaaaaagg
    19141 gctgcccgca gtctgctcag gtcgcgatta ttgctgatgt cgacgagcgt acccgtaaaa
    19201 caggcgaagc cttcgccgcc gggctggcac ctgactgtgc aataaccgta catacccagg
    19261 cagatacgtc cagtcccgat ccgttattta atcctctaaa aactggtgtt tgccaactgg
    19321 ataacgcgaa cgtgactgac gcgatcctct gcagggcagg agggtcaatt gctgacttta
    19381 ccgggcatcg gcaaacggtg tttcgcgaac tggaacgggt gcttaatttc ccgcaatcaa
    19441 acttgtgcct taaccgtgag aaacaggacg aaagctgttc attaacgcag gcattaccat
    19501 cggaactcaa ggtgagcgcc gacaatgtct cattaaccgg tgcggtaagc ctcgcatcaa
    19561 tgctgacgga gatatttctc ctgcaacagg cacaaggaat gccggagccg gggtggggaa
    19621 ggatcaccga ttcacaccag tggaacacct tgctaagttt gcataacgcg caattttatt
    19681 tactacaacg cacgccggag gttgcccgca gtcgcgccac cccgttattg gatttgatca
    19741 tggcagcgtt gacgccccat ccaccgcaaa aacaggcgta tggtgtgaca ttacccactt
    19801 cagtgctgtt tattgccgga cacgatacta atctggcaaa tctcggcggc gcactggagc
    19861 tcaactggac gcttccaggt cagccggata acacgccgcc aggtggtgaa ctggtgtttg
    19921 aacgctggcg tcggctaagc gataacagcc agtggattca ggtttcgctg gtcttccaga
    19981 ctttacagca gatgcgtgat aaaacgccgc tgtcattaaa tacgccgccc ggagaggtga
    20041 aactgaccct ggcaggatgt gaagagcgaa atgcgcaggg catgtgttcg ttggcaggtt
    20101 ttacgcaaat cgtgaatgaa gcacgcatac cggcgtgcag tttgtaatgc ataaaaaaga
    20161 gcattcagtt acctgaatgc tctgaggctg atgacaaacg aagaactgtc taatgcgtag
    20221 accggaaaag gcgttcacgc cgcatccggt cactttcagt tttactcttt ctcggagtaa
    20281 ctataaccgt aatagttata gccgtaactg taagcggtgc tggcgcgttt aatcacacca
    20341 ttgaggattg cgcctttaat attaacgcca gcctgttcca gacgctgcat tgacaagctc
    20401 acctctttgg cggtgttcaa gccaaaacgc gcaaccagca ggctggtgcc gacagagcgt
    20461 cccacgaccg cagcattgct caccgccagc atcggcggcg tatcgacaat caccagatcg
    20521 taatggtcgt tcgcccattc cagtaattga cgcatccgat cgcgcatcag cagttcagac
    20581 gggttaggtg gcacctgacc gcgagtaatc acatcaaagt ctcctttgcc aaaatgctgg
    20641 atcactttgt tgagctcatc tttacctgcc agatattccg acaagccatg ttcattactc
    20701 acggtaaaca ggttatgcga ataaccacgg cgtaagtcgg catcaataaa taacactttt
    20761 tgatcggact gggcgatcac cgctgccaga gttgaactga caaacgtttt accactgtct
    20821 ggcgtcgcac cggtgatcat cagaatgtta ttctcagtct ccatcatagc gaaatgcaga
    20881 ctggttcgta gcgcacgtac ggcttccaca gcagaatccg ccgggttatc caccgccagg
    20941 aaggggatat ttttagtacg atggcgctgc tgattagaaa ataaattttt cttacgcaga
    21001 cgggtgcgtt tatccagcca ctcggacatt gggatagtgg aataaacgct gatgccgtgc
    21061 tcttccagtt gttccggggc ttctacacca cgacgcaaca tcgcacgcgc cagcacggca
    21121 ctcaccgaaa taaacaggcc aagaataaaa ccaagcacca cattcaacgc ttttttcggt
    21181 ttcactggct gcggctgagt gactgccggg tcgataatcc gcacgttacc aatggcactg
    21241 gatttcgaaa tactcaactc ctgctggcgg ttaagtaatt gcagatatac cgcacggccc
    21301 gcttctacgt cacgacttaa acgcaacact tcctgttggg tggaaggcat tgccgatacc
    21361 cgcttattca ggcgtttgcg ttcttgctcc agcgtctggc gtttttccag cagcgcacga
    21421 taagttgggt gatctttctt atacagctgg gagatctctg cctcgcggaa ggtcagctca
    21481 ttgagttgat tatcaacgtt cacaatctgc tcaagaacgg ctttggcttc caggttaagg
    21541 tcaaccgaat cgcgctgctg gcgataaacg ttgagttttt cttccgcctg gtccagctcg
    21601 ctgcgcactt caggtaactg gcgctgtaag aattcaaggc tttgtgaatc ctgcgccgcc
    21661 tggcgagcga tattctgttg caaatagttg ttagcgatgc tgttcagaat acgagtaatc
    21721 aactggggat catcaccagt catggtaagt tccagcatcc cgctttcttt actgcgttcg
    21781 ctaacggtaa aggtttcctg caatgcgtta atcgcttcca gttcggtacg ctggctcagg
    21841 acaaactgtg ttcctggttt ggccttaatg tccgcgatag tcagcgcaac gccatctttt
    21901 tccagacgct ggccgaccat accattgacg gtgaactctt caccttccag tgtatagtgg
    21961 ccgttttccc caaccgtgag tgtcagttgc tgatcctgac cattcagttg tggaatatgc
    22021 atccagctga tcgccagctc acctagtttt tctttggtta atctcgccca gccgcgaccc
    22081 acaatcggaa aatacttctg ctcaactatg tcgcgcagat tcagttcagc aatggtttta
    22141 ccgagaatca tgcgcgattg cagcagttgg atctccggtg cagactcggg cgatgagtta
    22201 gggatcatat cgctcaggcc gctgagaatg gcgttgccct gtttttgctc aacctggacc
    22261 agagtatctg cctgataaat tggtgtgctt aacagcgatc agcgtgaata acgcggtcac
    22321 gctgataata aacttacggt gatcccataa ctcgccgacc agacgaagca gatcgatctc
    22381 attttcctga gtgctgcctg gtggcgtatt catattttta gttgtcatcg tattttataa
    22441 actcaaatta ccggctgagg cgcttcgccc attcctgact ggcgcgctcc aacatactgt
    22501 agacatgttc aaatgcgtcc tgacttttac gatagggatc cgggatctct ttctgttcca
    22561 gccattgccc aaacagcatt gtcttcccgc ggacctcggg agcaattgcg gtaacctgag
    22621 caatatgttc cgactccatt gccagaatca gatcgtaatt tcgtgccatc tcagcagtga
    22681 gcttacgtcc ggcatgtcct tcaagagata cgccatgatt agcggcaaca tctgccgctg
    22741 tcgcatctgc agggtgtttt accagaccat gaacacctgc cgatttgact tttacgcccg
    22801 gcagacgttt acgcagcaag cgctcgccaa tgggcgaacg gcaaatgttg ccggtacaaa
    22861 ccactaggat tgagttaaat tttagttggg ccatgtttga atccaccgtg aagtctctgt
    22921 caggtcatgc accccggaaa tcgtcggtac cagcagagaa atcacacggt tccaacgagc
    22981 cagcggcgcg gtagtgacat aaacaatgtc gtaaggttca agctggaatt ccgttccgag
    23041 tatcattgcc gatgcgtcct tcgcattcag ctgataaata ttggcgattt tgccattctg
    23101 cttgttttgc gttgcacgaa taacaaaaat ccccgtcgca tcagcgacat cctgattcat
    23161 accctcagcg ttacccagcg cttctgccag ggtcataccg ctgcggtcca ttttcagcgt
    23221 gctctgttta cccacttcac ccatcacgaa gactttcaga tcatcgttgc gcggaataaa
    23281 taaaatatcg cccgggtgca gcagtttgtt ctggcgcaag tcgccacgct gcatcagcgc
    23341 atagaggttc actttggtct ttacgccatt ctgggtgagc accacgttgc gccagtctgc
    23401 atctgcggtc agaccgcccg cggcgttaat cgcatccatg atggtcagag gaatattggt
    23461 aatcggctgc tgacccgatt tgctgacttc gccggtgacg tacgcttttt gcgatcggaa
    23521 cgccgcgacg ctgacatcga cttgcgggct ttcaatgact gaatcgagac gcgctgtaat
    23581 ctcgttgcgt acctgggtaa gtgttttccc ggccactttc aggcgaccaa tataggggta
    23641 gaaaatcgca ccatcggcgt taacccagtt gccggtatca ctggcgctac ggtattgccc
    23701 tgcgggggtg gttagttccg ggtgatccca gaccgtaacc atcaacacat caccaatccc
    23761 gatacgatat tcatagtttg cgatttgctg ctccagctcc gggttagcct gcgacataat
    23821 cggtttggcg cgcagctgat caatcaatcc tggcgtgacg ggatagatat tgaccatttt
    23881 gtccaggtca tactgattgt caggtagttc aatgacatcc ttgttactgg tcgataaatt
    23941 ctgaccagga acaagggtac atgctgtcaa agacagaaca gccaatgtca gtaccgaaaa
    24001 cttaaaaatg ttctttttca ttggtttata tttatcagtc aatgtaatgt tatgaattac
    24061 gtcaggttat tgtccaaccg gtacttctcg ctctgccgtc attggatcga gctggtattt
    24121 gcgtgacagc atttgtccac catcacgcgt caatggtgtc cacgaaacca ccgcgcggtt
    24181 gcggttaggt ccaatggtca tcaagtcgaa cggaatcgag atataaaaac ctttactaaa
    24241 gccgccttcg ccgtagtcat ctttagatac gttactgatt gccgcccata cccctaccgc
    24301 cacgccgctg tcaaagagtt tggcgacgtc gatcgttgcg cctttatctt ttgccagata
    24361 ttgcccaacg ctaagtttca tcagtacgcc attgagcgtc ggcgggttcc agtaagccgt
    24421 cacgaaacct gtgggcgtgg aataatcggt gaagcgcatc atgttgtccc agtcacgctg
    24481 cttcacgtag ttaacgtcca cacccagcgc ccagctggca tctagcgggc gataaagaag
    24541 ctcggaaccg acaccggcat acatcgtttc cagataaccg ccatacacct ggccatagaa
    24601 accattgcct aagtcggcaa agtaattcgc ttgcaagttg ttgagataaa cgtcattgcg
    24661 aacgtaatca cggatatgcg tgcgcacgcg gggcagggta gagtccgcgg gtaatagtga
    24721 agacttaaac ttgtcgtagt tgttgtaaat attggtgaaa ataccgtcat caagcagcag
    24781 gtggtcggta aaccagtagc gggcgctgga catcagcccc agctggaaca tatagaaatc
    24841 ttccgggccg cccagcgact gtgaaagtgt tgggttgaaa gagtagctaa agcgatcttc
    24901 acgaatacgg tagcaccgac caaaggcaga aagatcttct gcttcaacac gttgttgttg
    24961 cagttgctct gatttaccag gcgctgtgcc ggccagctgt ttgcgcaggc tggctacgtc
    25021 ggtttcggta gtcaccatcg ccatatgctc gcgcttttgc gtcacgctaa tcttctcaac
    25081 gccttgcggc aggttattca ccagaatccg gttggcacga tcgaccgctt cgcgagagtc
    25141 acggtatttg tattgctgac cagacatata cagcgtctta tcgcgtagct gaatttctgg
    25201 cgcgtcaaag cccgcgttat acttcagggc ggtaagttgg tttgctaccg tggtgtactg
    25261 caaaccttca gactctggcg caggctgcca ggccggtttt ggcgtatcgc gtagggcagg
    25321 gcgcagatcg ttgaaattgg tccgtaaagt gaaaccaaac atcaacgtgt ttccgcgttc
    25381 ataactgagg ttgaggtctg cccagctggc agcgcgataa actgcaccga cgttgaaatg
    25441 gcttgcctga ggcagtttgc cagcgaaatc attctggtaa ttgttgccgt cgtattcgag
    25501 tttcagacgc aggggattcc acggcgtttg atactcaatg ccgccaaaga tggaagccgg
    25561 gccacgaaag atatcgctaa agctgatatc gcccgcatcg tgagactctg cgcgatgaca
    25621 atatttatcg ctcacccggc aaaacgggtt ggtaatattg cccgcattac cggcgtagcc
    25681 ccatgccatc ccgagggtga aatcaaatgg ccccgcttgc ttgctggcca ccagatattc
    25741 accgtcaaac aaacccgtac cagcaatatc acgtttacca aacgccactt gcggtagcca
    25801 gtaaccttct tcccacaggc gaagtttaaa atcgaatgat ttgtctttgt atgactgatc
    25861 gccgctgaaa tcttcccact ggctatattt gcgtgtgcgc acatccgtat aacgaatggt
    25921 gccttccagc catgggaaca gcgccacgga ggtggagtag aaccggtatt gatcgttatc
    25981 ccggtagttg acgctgaatt caccttccgg tgcgatgcgc gcatttggca tctgcaacaa
    26041 tcctgtgccg ccaaagtctg attgcgacga acccagcgga tccgggtagg ttaatacttc
    26101 agcatgacag gcactggaga cggcaatggc caggcagctt aaaagataag aattcttctt
    26161 catcagtcag gaacacgttg cgttaaaaca gacacaatct ggtcatttaa atcagcatat
    26221 ttttcgggca gtacatgcgc agaaaagccc aaccaaagct ggctgccagg cggtggttcc
    26281 acatgtcttt tattccacag ggcaacaggg gcgacgacag tctcaccctc aggcgtaatc
    26341 accataacgt tattcttgtc tgcgcctgca aggcgggggt gatcttgcag ataatcggta
    26401 acgctacggc ctgcctgcca gggcaattgc ccagccccag aaaccgcacc cagcagagta
    26461 atagtcaccg gacgctgtac ggtatagagc gtatagtccc cgaccaacgg gggattgctg
    26521 ttctcatcca ctcgtacaaa atcagggtcc agtttgaccg gtaagcgacc agtgatattg
    26581 agattgagta actgctggcg cacggattta atcgttgccg ccacatcgtc gtcagcttct
    26641 gcttcccagg aagcaagctg tgccatgaca tgttggtagt ctttaagcgc tttagctttc
    26701 gccgcagagt cggtcagcaa ggcaccaggc caccagaggc gatcgcgcag ttgcggttgt
    26761 gtcaccaact ggacaacatt ttccacgggg ccaacggaaa gtgtctgttg ttcgctaggc
    26821 agataaatag tcaccgttcc ttgcgcaaag gcatggggtg tcattacgta aagtacgctg
    26881 gcaatgaaat acgactgtaa tttattcatg gtgcgggttt aagaaacgtc atttctacgg
    26941 gaataacccc tgcgcctaac atttgccgac tttgacgaac ctggccagta gccgaatcga
    27001 cccagaatgt gttttgccac tgtttttctg gcagcgtgga agtcacctct tcctgccaga
    27061 cattacacag cacattgtga cccgcaatat tcagcgtctc taatccagca aaggaaaaag
    27121 tagaacttag ggtcgcagag cgaaaatggt tgtcttcact ccagagaata tcgcgcgtcc
    27181 agcggctgcc atcttttatt gccagcgcgt tacgcagagg ggcccgcccg ggatttgtca
    27241 cttccagcag attattatta agtttgacgg tctttaaaag acgtccattg tgggtaacca
    27301 gcatggcgtt atcctgggat aaccatttgc tttgttcttg ttctatatat cccagtacca
    27361 caaagattcg ttgcccttca ttaaggcgta aatacatcgt ggaataaggc aacgcctgga
    27421 tctgctgagc cgctacagtg atatcctgat tatcgaaaag gctggcgcga aatgtatcga
    27481 ccatactttg ctggctgtgc gtacatcccg caagaaatag tgcgaaaatc gataaaataa
    27541 gagggcgcac ctttaatcct tgaaaggtta attaaaaaaa tgcaaatctc taaagcccgg
    27601 tacgaatatc gcaaccgggt attatttaac ttgaatttcg acgcgtttaa gcagaattag
    27661 cgcgtagtgg acgtggtggt tgaggtgtta tgagaaccgc catcgccact gtcatttgct
    27721 gcaactactg ccaacgtcgc cacaccaaca gccgttgcca caccgacaac tgtcgccgtg
    27781 ccgacagcgc cagcagccac gccactagtg gtagccgcag tcgtggttgc acctgtagtg
    27841 gtcgttgtgg tgccagtagt acccgtcgtt gcctcggtcc cgtttgctgc ttcaggggca
    27901 gcaaacgcag ctggcgtcgt caacatgaag gccataagga ttgcagaaag cttgtgtttc
    27961 ataacttttc ctttattcat cgcatggaca atacgtacgc ttgagatcag gattgtctac
    28021 tttgagtata acatccttgt gaaatctccc tttcctcata ccgctagcac tatatactgc
    28081 gggtgggaaa ggcgcgcaga gggaaatata agattgttaa cgatttccct ctggaaatac
    28141 gacttgtgag cataagataa atcttagaaa aatacgaatt aaagataaga aaattgttac
    28201 gaaagctatt aattattgtt ggtaatatag tttcaagtgg tactataact ggctgttaaa
    28261 tggcatttat aactattagg tgcagagata ttcgcttaaa tggattagtt tcttagtgga
    28321 aaagtaactg atgttataat gtatggtgtt gactattgaa ctattccaat aaaaccagtc
    28381 agctttaaac aagcagcgtc atattaagag agataaacat ttgccgctgt tggtcctcgc
    28441 aggccattta cgcggcaaaa ttccacgcgt aatcctggta taagcacttc tgcgtcgcgg
    28501 ggagtgaatg cggaaatatg gacctggact tctttacgac cgtcggaggg gataatgaat
    28561 cctttgccgc ttttgcgatc aaaggttttg acaattcctg tcattttacg ggacaaacaa
    28621 attccttact gaaaatactg cgctgcacta tacggggtta ataaaataaa gccagcgata
    28681 tttaagaccg ccggacggct aaaataaaat ttgcttaatc tcaattatca tgccttaata
    28741 gctgcgtcgg tttgaaagac
//
LOCUS       DAJTYM010000048        28602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTYM010000048 DAJTYM010000000
VERSION     DAJTYM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..275
                     /locus_tag="OO575_002123"
     CDS             <1..275
                     /locus_tag="OO575_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6665083.1"
                     /translation="TNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            297..1706
                     /locus_tag="OO575_002124"
     CDS             297..1706
                     /locus_tag="OO575_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6665084.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            1684..2463
                     /locus_tag="OO575_002125"
     CDS             1684..2463
                     /locus_tag="OO575_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6665085.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2460..3320
                     /locus_tag="OO575_002126"
     CDS             2460..3320
                     /locus_tag="OO575_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6665086.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3468..4043)
                     /locus_tag="OO575_002127"
                     /pseudo
     CDS             complement(3468..4043)
                     /locus_tag="OO575_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4060..4320)
                     /locus_tag="OO575_002128"
     CDS             complement(4060..4320)
                     /locus_tag="OO575_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6665087.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4311..5582)
                     /locus_tag="OO575_002129"
     CDS             complement(4311..5582)
                     /locus_tag="OO575_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6665088.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5660..6022)
                     /gene="queD"
                     /locus_tag="OO575_002130"
     CDS             complement(5660..6022)
                     /gene="queD"
                     /locus_tag="OO575_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6665089.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6341..8140
                     /gene="cysJ"
                     /locus_tag="OO575_002131"
     CDS             6341..8140
                     /gene="cysJ"
                     /locus_tag="OO575_002131"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6665090.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8140..9852
                     /gene="cysI"
                     /locus_tag="OO575_002132"
     CDS             8140..9852
                     /gene="cysI"
                     /locus_tag="OO575_002132"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6665091.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            9926..10660
                     /gene="cysH"
                     /locus_tag="OO575_002133"
     CDS             9926..10660
                     /gene="cysH"
                     /locus_tag="OO575_002133"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6665092.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            10924..11076
                     /locus_tag="OO575_002134"
     CDS             10924..11076
                     /locus_tag="OO575_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6665093.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11270..12361
                     /locus_tag="OO575_002135"
                     /pseudo
     CDS             11270..12361
                     /locus_tag="OO575_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12507..13544)
                     /gene="iap"
                     /locus_tag="OO575_002136"
     CDS             complement(12507..13544)
                     /gene="iap"
                     /locus_tag="OO575_002136"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6665094.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            13796..14704
                     /gene="cysD"
                     /locus_tag="OO575_002137"
     CDS             13796..14704
                     /gene="cysD"
                     /locus_tag="OO575_002137"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6665095.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            14706..16133
                     /gene="cysN"
                     /locus_tag="OO575_002138"
     CDS             14706..16133
                     /gene="cysN"
                     /locus_tag="OO575_002138"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6665096.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16133..16738
                     /gene="cysC"
                     /locus_tag="OO575_002139"
     CDS             16133..16738
                     /gene="cysC"
                     /locus_tag="OO575_002139"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6665097.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            16788..17111
                     /locus_tag="OO575_002140"
     CDS             16788..17111
                     /locus_tag="OO575_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6665098.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17305..17616
                     /gene="ftsB"
                     /locus_tag="OO575_002141"
     CDS             17305..17616
                     /gene="ftsB"
                     /locus_tag="OO575_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6665099.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17635..18345
                     /gene="ispD"
                     /locus_tag="OO575_002142"
     CDS             17635..18345
                     /gene="ispD"
                     /locus_tag="OO575_002142"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6665100.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18345..18824
                     /gene="ispF"
                     /locus_tag="OO575_002143"
     CDS             18345..18824
                     /gene="ispF"
                     /locus_tag="OO575_002143"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6665101.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            18821..19870
                     /gene="truD"
                     /locus_tag="OO575_002144"
     CDS             18821..19870
                     /gene="truD"
                     /locus_tag="OO575_002144"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6665102.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            19851..20612
                     /gene="surE"
                     /locus_tag="OO575_002145"
     CDS             19851..20612
                     /gene="surE"
                     /locus_tag="OO575_002145"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6665103.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20606..21232
                     /gene="pcm"
                     /locus_tag="OO575_002146"
     CDS             20606..21232
                     /gene="pcm"
                     /locus_tag="OO575_002146"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6665104.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21372..22511
                     /gene="nlpD"
                     /locus_tag="OO575_002147"
     CDS             21372..22511
                     /gene="nlpD"
                     /locus_tag="OO575_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6665105.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22574..23566
                     /gene="rpoS"
                     /locus_tag="OO575_002148"
     CDS             22574..23566
                     /gene="rpoS"
                     /locus_tag="OO575_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6665106.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(23686..24093)
                     /locus_tag="OO575_002149"
     CDS             complement(23686..24093)
                     /locus_tag="OO575_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6665107.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24240..24833
                     /locus_tag="OO575_002150"
     CDS             24240..24833
                     /locus_tag="OO575_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6665108.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            24833..25780
                     /locus_tag="OO575_002151"
                     /pseudo
     CDS             24833..25780
                     /locus_tag="OO575_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26053..26616)
                     /locus_tag="OO575_002152"
     CDS             complement(26053..26616)
                     /locus_tag="OO575_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665109.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            26781..27032
                     /locus_tag="OO575_002153"
     CDS             26781..27032
                     /locus_tag="OO575_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6665110.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27034..>28602
                     /locus_tag="OO575_002154"
     CDS             27034..>28602
                     /locus_tag="OO575_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6665111.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEED"
BASE COUNT         6949 a         7110 c         7749 g         6794 t
ORIGIN      
        1 gcaccaacat gtacttcgtc tatgactaca acgttgttga ctgtaagccg gaagaggaaa
       61 tcgacaagta ccacaatccg ctcaacaaaa tcatctgcga agaaaccatt cgcctcggcg
      121 gttcgatggt gcaccaccat ggcattggta aacatcgcgt tcactggagc aagctggaac
      181 acggcagcgc gtgggcgttg ctggaagggc tgaaaaagca gttcgatcct aacggcatta
      241 tgaatacggg tactatctat ccgattgaaa aataatgtat caggcagcgt tccgcgatga
      301 cggggcgttg ccttttcggc ttctcaggcg agaagccgtt cttattaccg gacaatgaag
      361 gggtaaagat gaacacttca ccggtgcgaa tggatgattt accgcttaac cgttttcact
      421 gtcgaattgc tacgctcact ttcggcgcac acctgaccga cggttatgtt ctaggcgtca
      481 ttggttacgc cattattcaa cttacgcccg ccatgcaact gacgccgttt atggcgggaa
      541 tgatcggcgg ctcggcgctc cttggtttgt ttcttggcag cctggttctt gggtggatct
      601 ccgaccatat tggtcggcaa aaaatcttca ccttcagctt tttgctgatt acgctcgctt
      661 cgttcttgca attttttgcc accacgccag agcatcttat tgggctgcgc attttgatcg
      721 gcattggtct gggaggcgat tactcagtag gtcacacctt gctggctgaa ttttccccgc
      781 gccgccatcg cggtattttg ctgggcgcat tcagcgtggt gtggaccgta ggctatgtgt
      841 tggcaagtat tgccggacat cactttattt ccgaaaaccc ggaggcctgg cgctggttgc
      901 tggcatcggc agctctgccc gcgttgttga ttacgttatt acgctgggga acgccagaat
      961 cgccacgttg gctactgtgc caggggcgtt ttgcagaagc tcacgctatc gtgcatcgct
     1021 attttggtcc ccatgtttta ctgggcgatg aagtggtaac ggcgacccat aaacacatca
     1081 aaaccttgtt ctcttcgcgt tactggcggc gcacggcgtt taacagcgtc ttctttgtct
     1141 gcctcgtaat cccatggttt gtgatttata cctggctgcc aactatcgcc cagactattg
     1201 gtctggaaga tgcgctgact gccagcctga tgcttaatgc gttgttaatt gtgggcgcgc
     1261 tgctgggatt agttctgacg cacctgctgg cacatcgcaa atttttgctg ggaagttttt
     1321 tgctgctggc ggcaacgctg gtagtaatgg cctgtttgcc ttccggcagt tcattaacgc
     1381 tgctgctttt tgttctcttc agcaccacca tttcggcagt cagtaatctg gtgggcattt
     1441 tgcctgcgga aagttttcct actgacattc gctcgctggg agtcggtttt gccaccgcca
     1501 tgagtcgact tggcgcggcg gtaagtactg gcctgctgcc gtgggtgctg gcgcagtggg
     1561 gaatgcaagt caccttattg ctcctagcga cagtgttgtt ggttggtttt gttgtgacct
     1621 ggctatgggc accagaaact aaagccctcc cgctggtggc ggcgggaaat gtaggaggtg
     1681 cgaatgaaca ttctgttagc gtttaaagcc gaaccggatg ccggaatgct ggcggaaaaa
     1741 gagtggcagg cggcggctca gggtaatagc ggaccggatg tttcgctact gcgaagttta
     1801 ctcggtgctg atgaacaggc cgctgccgcg ctgctgttag cgcagagaaa aaacggtacg
     1861 ccgatgtctt taaccgcgtt gagcatgggg gatgaacggg cgttgcactg gctgcgctat
     1921 ctcatggctc ttgggtttga ggaagctgta ttgctggaga cggcggcaga tctgcgcttt
     1981 gcgccggaat ttgtcgctcg ccatattgcc gaatggcagc gtcagaatcc gctggatctg
     2041 ataattaccg gcagccaaag cagcgaaggg cagaatgggc aaacgccatt tttgctggcg
     2101 gaaatgctgg gctggccctg cttcacccag gtggagcgtt tcactctcga cgcgctgttt
     2161 atcaccctcg aacagcgtac tgaacatggg ttgcgctgtt gccgggttcg cttgcctgcg
     2221 gtgattgccg tgcgtcagtg cggtgaagtg gccttgccag taccgggaat gcgccagcga
     2281 atggctgcgg ggaaagcaga aattatccgt aaaactgttg ctgccgaaat gccagcgatg
     2341 cagtgtctgc aactggcaag agcagagcaa cgacgcggag cgacgctgat tgatgggcaa
     2401 acggtagcag aaaaagctca aaagctatgg caggactatt tacgccaaag gatgcagcca
     2461 tgaatatcgc aattgtgaca ataaatcagg agaatgccgc aatagcgagc tggctggcgg
     2521 cgcaggattt cagcggttgt acgctggcgc actggcaaat agaacctcag cccgtagtgg
     2581 cggaacaggt gctggatgcg atggtggaac agtggcagcg aacgcctgcg gacgttgttc
     2641 tctttccgcc gggaacgttt ggcgatgaac tgtcgacccg gctggcctgg cgtttacatg
     2701 gtgcaagcat ctgtcaggtg acttcgttag atattccaac cgtaagcatg cgcaaatccc
     2761 actggggaaa tgcgttaacg gcgacgctgc aaacagaaaa acgcccgtta tgcctttccc
     2821 tggcgcgtca ggcgggagca gcgaaaaacg ccacgttgcc ctctggaatg cagcaactga
     2881 acattgtccc aggtgcgctt cccgactggc tggttagcac agaggatctg aaaaatgtta
     2941 gccgcgaccc attagctgag gccagacggg tgctggtggt gggacagggc ggagaggcag
     3001 ataaccagga aatcgttatg ctggcagaaa agctgggagc cgaagtgggc tacagtcggg
     3061 cgcgagtgat gaacggtggc gttgatgctg aaaaagtgat tggtatttcc ggacatctgc
     3121 tggcacctga ggtgtgcatt gttgttggcg cttccggcgc agcagcgtta atggcgggcg
     3181 tacgcaatag caagtttatg gtggcgatta atcatgacgc cagcgcagcg gtattttcac
     3241 aggcagatat tggcgtggtt gatgactgga aagtggtgct ggaggcactg gtgacaaaca
     3301 ttcacgcaga ataccaataa cgtgctgccg gatgatcctg cctacggttc tggactgggg
     3361 acctgtaggc ctgataagac gcgttaagcg tcgcatcagg cgctgaatgt cggatgtggc
     3421 gtgcggccaa caaatcaaaa caaattcaat tggttacgat tatcccgtca aagcaatttt
     3481 ttcgctaacg tccagactcc ggtattcgtg gtggaaagcg ccacgacacc cgcgttaatc
     3541 gcattacgcg catcttcttc atcgcatacc agcccgccag caatcagcgg ttggcggatt
     3601 ttttccgtca cccagcccag cactttgggc atgcagcctg gcaggatctc aatacaatcc
     3661 ggattcgatt gcgcaactta cttatcaatg ttgtgaaacg aaatcgaatc aacaataaac
     3721 aggtgatgaa tacagaaaaa aacttccgct cttgccgctt ttagcatcga tgctttggtg
     3781 ctgataattc catccgcctc ggtcaccagt ttcaaaaact gaatcaccac ttctttattt
     3841 gacgcgcctt ccagcaaatc aacatgaata aaagcatatt tcccggcgtt cttaatcttt
     3901 ttaacgatat tactgatggt gcagatattg ccgtacaaca cggaaataaa ctggcattca
     3961 gaatcgatgg ccagttgcag gctggcatta tctttaacgg cagcaatcac cggattctgg
     4021 cggagcaggt gtaagagggg cataaattat ccttattatt tatccaaagc ggtaggtgat
     4081 gccgaatccg gaaggcggat agcgccactg ttgtagtgtg gattcgtcgc acagcaaacg
     4141 gcaggtgcca cactccaggc agccgcgata gtcaatacgt aagtcacctt cctgtgtgag
     4201 agaaaaaaga cctaccggac aggcgttaat caaccgttgt accatctgac gctcaacgga
     4261 gtcagccgga acaatgtgcg gcgcatcagc aacgcgccag agattacggg ctacagacat
     4321 cgcagactcc tgataacatc gcccgccaga tgccacaggc catgacgacg taaatgatgc
     4381 cagaataact ggcgcagcgg tggaacaggt ttatcaccct gatcccataa atcgcgggaa
     4441 atatcctgca ttaacgcagg ccacgcccga tacctgccag ggcgttgcaa aagcgccgga
     4501 acatgctgat aacgttgtag aacatcccat agcaggctgc gctctacgtt gtgatgataa
     4561 agcgcaaaca gattttgcgg ctcgcggtgc tggcaggcgc ttattagcgt ttgtgccgcc
     4621 gcctgtgcgc cagtcagcgc catatccatg ccgcgcacgg aaattccggt attgacgcaa
     4681 ctgcgcaacg catcgcccac cagcagccag ccgttaccgg cgtattgcac cggcatactg
     4741 tgcaagccac cttctggcac cagatgcgca ccatactcca gtgattccgt gtttttgata
     4801 agcgtgcgca ctgccggatg cgctttaaag cgagtcagca gctcgcttgc cggaacacga
     4861 ctttgcgtaa gggaagagag cgggcaaaca atccctaacg agagcgtttg ttgattagta
     4921 taaagaaatg cgccgccggg taagtcatca cagattccgc cgctgaacag caacgctgcg
     4981 ccttcgttat tctccagatg aaaacgttct tcaatagcgg atgtttccag cgacagcact
     5041 tctttgatcc ccaacgccat cgcttcgcca gcaggacgag tcactaaccc gtgacgttca
     5101 gccaggacgc tgttggcacc ttctgccagc accacataac gggcgcggag aatatcgtca
     5161 ccacaaataa cgccacagac tctgccgttt tcttcataca gtgcatccac cgtcgctccg
     5221 gggatgcatt cgacaccttc tttttcggct tcggcaacca gccacggatc gaaccgtgca
     5281 cgtaatacat tccaggattc accgccgggc tgtaagctgg aaaacgtcgt tgcgccatcc
     5341 ggcgttaaca gggaaaggct ttcgtgagtg atgcgtcgtt caagaggcgc ggtcagatga
     5401 aattgcggga ggagttccgc gagtgcatgg gtatataacc gcctgccgga aagatttttg
     5461 ctgccgggga tttcagcgcg ttcgagtaac aaaacggata aatccgctcg cgcgcagcgt
     5521 aacgcgcaag cggtgcctgc aataccggca ccaataataa taatgtcgca gtcgtcttcc
     5581 atgtcatgcc ccagatatcc agaacacaac accctaacat agcgttacta aagggaaatt
     5641 gaccgccgac acactcttat cattcgccgc gatagataca ccctgcggtg caggtttctt
     5701 ttaccatcac cgcacttaac agcggcacaa cgggtttaac ctgatcccaa atccattttg
     5761 ctaaaacttc gctggttgga ttttccagac ctggaatatc attgagataa tggtgatcga
     5821 ggcgctcata agttggctta aacgccgctt tcagttcagc gaaatcgata atccagcccg
     5881 tatgcggatc gacttccccg gtaatttcca gtcgcaccat aaaggaatgc ccgtgcaggc
     5941 gaccacattt atgcccttcc gggacatgtg gtaagcggtg agcggcttcg aaggtgaaat
     6001 ctttaaataa cgtggtggac atcataattt ctctacagga cgataaaacc gccgtagagt
     6061 accggaaagg ctgttttttc gcattatcta aaacgacttt gttgcgcaaa atcgctgatt
     6121 tatcttaatg attggctaaa ttcatttgtt tttcattagg ttggttaatc tattttgttg
     6181 ttaaagacta ttgctaaaac aggttagtcg atttggttat tagttatcgc tatcccgtct
     6241 ttaatccaca ccgttcaccc cgttaacctt accttctctt ctgttttatg ggcgctgaca
     6301 gggcgcagaa acagctttgc ttactggaac ataacgacgc atgacgacac aggtcccacc
     6361 ttccgcgttg cttccgttga acccggagca actggcacgc cttcaggcgg ccacgaccga
     6421 tttaacgcct acccagcttg cctgggtttc tggctatttc tggggcgtgc tcaaccagca
     6481 gcctgttgcg cttgcagtga cgccagcgcc cgccgcagaa atgccgggta taacgattat
     6541 ctctgcttcg caaaccggca atgcgcgccg ggttgctgaa gcattacgcg atgatttatt
     6601 agcagcaaaa ctgaacgtta agctggtgaa cgcgggcgac tataaattca aacaaatcgc
     6661 cagcgaaaaa ctgctcatcg tagtgacgtc aacgcaaggg gaaggggaac cgccggaaga
     6721 agccgtcgcg ctgcataagt tcctgttctc caaaaaagcg ccaaagctgg aaaacaccgc
     6781 gtttgccgtg tttagcctcg gcgatagctc ttatgaattt ttctgccagt ccgggaaaga
     6841 ttttgacagc aagctggcgg aactgggggg tgaacgcctg ctcgaccgtg tcgattctga
     6901 tgttgaatac caggctgctg ccagcgagtg gcgcgcccgc gtggttgatg cgcttaaatc
     6961 gcgtgcgcct gtcgcggcac cttcgcaatc cgtcgctact ggcgcggtaa atgaaatcca
     7021 caccagcccg tacagcaaag acgcgccgct ggtggctagc ctctctgtta accagaaaat
     7081 tactgggcgt aactctgaaa aagacgttcg ccatatcgaa attgacttag gcgactcggg
     7141 cctgcgttac cagccgggtg acgcgctggg cgtctggtat cagaacgatc cggcactggt
     7201 gaaagaactt gtcgaactgc tgtggctgac aggcgatgaa cctgtcactg tcgagggcaa
     7261 aacgttgcct ctgaacgaag cgctacagtg gcacttcgaa ctgaccgtca acaccgccaa
     7321 cattgttgag aattacgcca cgcttacccg cagtgaaaca ctgctgccgc tggtgggcga
     7381 taaagcgaag ttacagcatt acgccgcgac gacgctgatt gttgacatgg tgcgtttctc
     7441 cccggcacag cttgatgccg aagcgctaat taatctgctg cgcccgctga ccccgcgtct
     7501 ctattccatt gcctcctcgc aggcggaagt cgagaacgaa gtacacgtca ccgttggtgt
     7561 ggtgcgttac gacgtggaag gccgcgcccg tgccggtggt gcctccagct tcctcgctga
     7621 ccgcgtggaa gaagagggcg aagtccgcgt atttatcgaa cataacgata acttccgcct
     7681 gcccgctaac ctggaaaccc cggtgattat gattggccca ggcaccggta ttgcgccgtt
     7741 ccgcgccttt atgcagcaac gcgccgctga cgaagcgcca ggtaaaaact ggctgttctt
     7801 tggcaatccg cattttacgg aagacttcct gtatcaggtg gagtggcagc gctacgtcaa
     7861 agagggtgtg ctgacgcgga tcgatctcgc ctggtcgcgc gaccaaaaag aaaaagttta
     7921 cgtacaagat aaactgcgcg aacagggcgc ggagctgtgg cgctggatca atgacggtgc
     7981 ccacatttat gtctgcggcg acgctaatcg catggcgaaa gacgttgagc aggcacttct
     8041 ggaagtgatt gccgaatttg gtggcatgga caccgaagcg gcggatgaat ttttaagtga
     8101 gctgcgcgta gagcgccgtt atcagcgaga tgtctactaa tgagcgaaaa acatccaggg
     8161 cctttagtgg tcgaaggaaa actgacagac gccgagcgca tgaagcttga aagcaactac
     8221 ctgcgcggca ccattgcgga agatttaaac gacggtctga ccggcggctt taagggcgat
     8281 aacttcctgc tgatccgctt ccacggcatg tatcagcagg atgaccgcga catccgcgcc
     8341 gaacgtgctg aacagaagct ggagccgcgc cacgcgatgc tgttgcgctg ccgtctgccg
     8401 ggtggagtta tcaccaccaa acagtggcag gcgatcgata aatttgccgg tgaaaacacc
     8461 atctatggca gcattcgcct gaccaaccgc cagacgtttc agttccacgg cattctgaaa
     8521 aagaacgtca aaccggtgca ccagatgctg cactcggtcg gtcttgatgc gctggcgact
     8581 gctaacgaca tgaaccgtaa cgtactctgc acctcgaacc cttacgagtc gcagctgcac
     8641 gcagaagcgt acgagtgggc gaagaagatt tctgaacatc tgctgccgcg tacccgcgcg
     8701 tatgcggaga tctggctcga tcaggaaaaa gtcgccacca ccgatgaaga accgatcctt
     8761 ggtcagactt acctgccgcg taagttcaaa accacggtag tgatcccgcc gcagaacgat
     8821 attgatctgc acgccaacga catgaacttc gtggcgatcg cggaaaacgg caagctggtg
     8881 ggcttcaacc tgctggtggg cggtgggctt tctatcgaac acggtaacaa gaaaacctac
     8941 gcccgcacgg cgagtgagtt tggctatctg ccgctagagc atacgctagc ggtggcggaa
     9001 gccgtcgtga caactcagcg tgactggggt aaccgaaccg atcgtaaaaa tgccaaaacc
     9061 aaatacacac tggagcgcgt gggggttgag acgtttaaag cggaagtgga gcgtcgcgcg
     9121 gggatcaaat ttgaaccgat ccgcccgtat gaatttacag gacgcggcga tcgtattggc
     9181 tgggttaagg gcattgatga taactggcac ctgacgctgt ttatcgaaaa tggtcgcatc
     9241 cttgattatc cggggcgtcc gctgaaaact ggcctgctgg agatcgcgaa gatccacaaa
     9301 ggtgatttcc gcattacggc gaaccagaat ctgatcatcg ccggtgtgcc ggaaagcgag
     9361 aaagcgaaga tcgagaaaat tgccaaagag agcgggttaa tgaatgccgt cacgccgcag
     9421 cgtgaaaact cgatggcctg cgtgtcattc ccgacttgcc cgctggcgat ggcggaagcg
     9481 gagcgtttcc tgccgtcttt tatcgacaac atcgataatt taatggtgaa acatggtgtc
     9541 agcgatgagc atatcgtgat gcgtgtaaca ggctgcccga acggttgtgg tcgcgcgatg
     9601 ctggcggaag tgggcctggt gggtaaagca ccgggtcgct acaacctgca tcttggcggc
     9661 aaccgcattg ggacacgtat cccacggatg tataaagaaa acatcaccga gccggaaatc
     9721 ctggcgtcgc ttgatgaact gatagggcgc tgggcgaaag agcgcgaagc gggtgaaggc
     9781 ttcggcgact ttacggtgcg tgcgggcatc attcgcccgg tgctcgatcc ggcgcgcgat
     9841 ttgtgggatt aaccatcagc ccggtcttgt aggcctgata agacgcgtga gcgtcgcatc
     9901 aggcaaggca aacagtgagg aatctatgtc caaactcgat ctaaacgccc tgaacgaact
     9961 gccgaaggta gatcgcattc tggcgctggc agaaactaac gccgaactgg aaaaactgga
    10021 cgctgaaggc cgcgttgcct gggcgctgga taatctgccc ggtgaatatg tactttcttc
    10081 cagctttggc attcaggcgg cggtgagcct gcatctggtg aatcagattc acccggatat
    10141 tccggtgatc ctcaccgaca ccggctatct gttcccggaa acctaccgct ttattgacga
    10201 gttaacggac aaactcaagc tcaacctgaa agtgtaccgc gctaccgaaa gcgccgcctg
    10261 gcaggaagca cgctacggca aactgtggga acagggcgtt gaaggcattg aaaagtacaa
    10321 tgacatcaac aaagtcgaac cgatgaaccg ggcgctgaaa gaactgaatg cgcaaacgtg
    10381 gtttgctggc ctgcggcgtg aacaatccgg cagccgtgcc aatttaccgg tgctggcaat
    10441 tcagcgtggc gtatttaaag tgctgccgat tatcgactgg gataaccgaa ctatttatca
    10501 gtacctgcaa aaacatggcc tgaaatatca cccattatgg gacgaaggat atttatcggt
    10561 gggcgatacc catacaaccc gtaaatggga acccggcatg gcggaagaag aaacgcgttt
    10621 ctttggctta aaaagggaat gtggattaca cgaagggtaa atattgccgg ataaattaaa
    10681 cgcctctgtc agaaatgatg ggggcgtttt tattttgcga gcagcattcc ggcatttaaa
    10741 ggtgaacatc gcagggaaaa ttaacaggta tagaatggcg ttgggtgctt gaggctgtct
    10801 ggcgttaagc attcgcgagg ttccagatgg acaaaagccc caggcgatat ttctatcaac
    10861 ctgaggctcg cgttcgaacg tagcaattcg gatgttagtc tcttctttgc agggaggcaa
    10921 gacatgctga caaaatatgc ccttgtggca atcatcgtac tgtgttgtac agtactggga
    10981 ttcacgttga tggtaggtga ctcgttatgt gagttgagta tcagagaacg tggtatggag
    11041 tttaaggcag ttctcgctta cgaatcgaag aagtagccac cacgcggggg taaaaccccg
    11101 cgtactgaat tgtgatggtt tggtctcctg cacccttttt ttaaatttat attttttatg
    11161 tgaatatata gtctctattt attattatga atttcaaatg aaaatcatta atttattcat
    11221 aatgtaaatg ttttattgac tgatatatat catagatagt ttttaaaata tgcgtaaata
    11281 tcctttaagt ttactgaagg ataaaaatat tgtgactttc tttgatttct ggggaaaaaa
    11341 ccgacgtggc gagaaagagg gtatcgacga ctatcacctt ctttgctggc atttgctgga
    11401 tgtggccgca atgggctatt taatggttaa aagaaattgc ttcgggctgg ctgattattt
    11461 tcgtcaatta gggatttctg acaaggaaca ggcagctcga tttttcgcct ggttgctgtg
    11521 ctggcacgat attggaaaat ttgcccgctc ttttcaacaa ctttacctgc cccctgaatt
    11581 caagattcag gaaggtgcca gaaaaaatta cgaaaaaatc tctcattcaa cgctgggcta
    11641 ctggttgtgg aatcattatt taagtgaatg tcaggagtta cttccttcat cttcgctctc
    11701 tccgcgcaaa cttaggcgag tgatagaaat gtggatgccc gtgacgacag ggcatcatgg
    11761 tcggccccct gatcgtatgg atgaattgga taattttctg cctgaagaca aagctgcagc
    11821 acgagatttt ctccttgaaa tcaagccact gtttccgcga atagagatcc ccacattctg
    11881 ggatgatgac gagggcgttg aacttataaa acaactttcc tggtatatct ctgcaaccgt
    11941 cgtacttgca gactggacag ggtcgtcaac gcgatttttt ccacgcgtcg cacaggcaat
    12001 ggatattgaa gattactggc aaaaggcttt agttcaggct caaaacgcat taaccgtctt
    12061 tccgccacaa gcagaaaccg cgcctttcac cggaattaat acgctgtttc cttttattga
    12121 gaatacgaca ccgttacagc aaaaggtgct ggatctggat atcaatcagc caggaccaca
    12181 gttgtttatt ctggaagatg tgaccggcgc aggtaaaaca gaagcggcgc ttatcctggc
    12241 gcacaggttg atggctgcgg gtaacgctca ggggctgttt tttggcctgc caacaatggc
    12301 aacggccaat gccatgtacg atcgactggt caaaacctgg cttgctttct attcgccaga
    12361 ggtgttcccc gctccagcgg agataaaccg gcgcaatacg gtgcggtttt cccggaagta
    12421 aaccggacgc actggatgcg atgatgggta tcacttagaa ttccccgccc ctgcggtaga
    12481 actcccagct cccattttca aacccatcaa gacgccttcg ccagctcctt caccaacggt
    12541 agcattatcc gcataacgtc acggcagcga cgttctattc ttccaggaag tgccttatca
    12601 atatgttgtt gattatccag tcttacgtca tgccagctat ttcccgccgg gaaggcaggt
    12661 gtttttgcgc gttgctgata accatcctta tttccaagat tccagttagt cgcttccacc
    12721 gaaagtacag caatgcccgc tttgtcgaat atttctgcgt cattacaacc cccagtgcct
    12781 ttcggataat ttttattcaa acccggattg gtcgttgcgg ctattccgtg actgcgtgca
    12841 attgccagcg ccctgtcgcg cgttaatttc cttactgctt caggggtttt tacaccgctg
    12901 ttgaaataca atttatcgcc aacaattaag ttatcgagat taatcaccag cagcgtattt
    12961 ttcttttcgg tgtcactcat ccgcttgagt aaattctcag cgcctaattt tccttcctct
    13021 tcgccgctgg tcgccacaaa tcgaatacca tactcggtag gcgtattttt caggcgttct
    13081 gccagttcca gcatgacacc taaacctgcg gcgttatcat ctattccttg taacgtcagt
    13141 ccgccgagat tggcatctgc gtcagcatcg ctcagcgggg cgtaagtatc cagatgcgcc
    13201 ataatgatga tctgctgcgg cgctttgcct tcatgagcgg caatcaccgt acttcctgtc
    13261 acgttatgcc agttcttgcg attatcgcgg gcggtataaa tataccgact attaaatgtc
    13321 cgaatatcac tgcgataacc catttgctga aactgttggc gaatataatc ggcagataac
    13381 atttctgctg gagttccggt catgcgtccc ggaaagaaag tagcaatatg tcgtgcctgg
    13441 gtattagcaa aatcgccagg tttaggtgac gaggcgtgta cggggagaat aaagcatacg
    13501 ccgagcgcca gggcagcggt acggtggcgc aatgcggaaa acatagtgag tccttaaata
    13561 ccatgcaaat ttttttaccg ccatagtatg aaactgccgc tgcgctaaaa caatttcaaa
    13621 tcttcctaaa cgcccgaaat ccggtgcctt aagcactttt tgatattagc tttgccaaat
    13681 cgttattccg ttaaggaact actcattcta attggtaatt tcattcgttc tcttacgctc
    13741 cctatagtcg aaacatctga tggcaagaaa atagcggtat tgcaaaggaa cggttatggc
    13801 tcaaaaacga cttactcacc tgcggcaact ggaggcggaa agcatccaca ttattcgcga
    13861 ggtggcggca gaattctcaa atccggtgat gctctactct atcggtaaag attccagcgt
    13921 catgctgcat ctggcgcgca aggcgtttta tccaggtacg ctgcctttcc cgttgctgca
    13981 tgtcgatacc ggctggaaat tccgcgagat gtatgagttc cgcgatcgta cggcgaaagc
    14041 ctacggctgc gagctgctgg tgcataaaaa cccggaaggc gtggcgatgg ggattaatcc
    14101 attcgtgcac ggcagcgcga aacataccga tattatgaaa actgaaggcc tgaaacaggc
    14161 gctgaacaaa tacggttttg atgccgcctt cggtggtgcg cgccgtgacg aagagaaatc
    14221 ccgcgctaaa gagcgaatct actctttccg tgaccgcttc catcgctggg atccgaaaaa
    14281 tcagcgcccg gagctgtggc acaactacaa cgggcaaatt aacaaaggcg aaagcatccg
    14341 cgtcttcccg ctctctaact ggaccgagca ggatatctgg caatacatct ggctggaaaa
    14401 tatcgacatt gtgccgctgt atctcgctgc ggaacgtccg gttctggaac gcgacggtat
    14461 gttgatgatg attgatgaca accgtatcga cctgcaaccg ggcgaagtga tcaaaaaacg
    14521 gatggtgcgt ttccgtacgc tgggctgctg gccgctgacc ggtgcggtgg agtcaaatgc
    14581 acaaacactg ccggaaatca tcgaagagat gctggtttcc accaccagtg aacgtcaggg
    14641 tcgcgtgatt gaccgcgacc aggcgggatc catggagctg aaaaaacgtc aggggtattt
    14701 ttaagatgaa caccgtactt gcacaacaaa tcgccaatga aggcggcgtc gaagcctgga
    14761 tgattgcgca acaacataaa agcctgctgc gttttctgac ctgtggtagc gtcgatgacg
    14821 gcaaaagtac cctgattggt cgtctgctgc acgatacccg ccaaatctac gaagatcagc
    14881 tctcatcgct gcataacgac agtaagcgtc acggaaccca gggcgaaaag ctggatctgg
    14941 ctctgctggt ggacggcctg caagctgagc gcgaacaggg catcactatt gatgtggcct
    15001 accgctattt ctctaccgag aagcgtaaat ttattatcgc cgacacccca gggcacgagc
    15061 agtacactcg caatatggcg actggcgcat cgacatgtga actggcgatc ttactgattg
    15121 atgcccgtaa aggcgtgctc gatcaaaccc gtcgtcacag ttttatctcc acactgttgg
    15181 ggatcaaaca tctggtcgtg gcgatcaaca aaatggatct ggtggattac agtgaagaga
    15241 cgttcacccg tattcgtgaa gattatctga cctttgccgg gcagctgccg ggtaatctgg
    15301 atatccgctt tgtgccgctc tccgcactgg aaggtgacaa cgtggcttcg caaagtgaaa
    15361 gtatgccgtg gtacagcggt ctgacactgc tcgaagtgct ggaaaccgtg gagatccagc
    15421 gagtggtgga tgcccagcca atgcgcttcc cggtgcagta cgttaaccgc ccaaatctcg
    15481 attttcgtgg ctacgccgga atgctggcat ccggtcgcgt ggaagtcggg caacgtgtaa
    15541 aagtgctgcc ctctggtgtg gaatcaaacg tcgcgcggat cgtgactttt gatggtgatc
    15601 gcgaagaagt ctttgccgga gaagcgatca ccctggtgct gacggatgag atcgatatca
    15661 gccgtggtga tctgctgctg gcggcagacg aagcgttacc ggcggtgcag agtgcgtcgg
    15721 tggatgtggt atggatggcg gaacagccgc tttccccggg ccagagttat gacatcaaaa
    15781 ttgccggtaa gaagacgcgt gctcgtgttg atggcattcg ttatcaggtt gatattaata
    15841 accttaccca acgcgaagtt gaaaacctgc cgctgaacgg catcggcctg gtggatctca
    15901 cttttgacga gccactggtg ttagatcgct atcagcaaaa tccggttacc ggtgggctga
    15961 tttttatcga tcgcctgagc aatgtgaccg tgggtgccgg tatggtgcac gagccagtta
    16021 gccaggcaac agctgcgtca tctgaattca gtgcattcga actggaattg aatgccctgg
    16081 ttcgtcgcca ctttccgcac tggggcgcgc gcgatttgct gggggataaa taatggcgct
    16141 gcatgacgaa aacgtcgtct ggcatagtca tccggtcact gtgcaacaac gcgaactaca
    16201 ccacggtcat cgtggtgtag tgctgtggtt taccggcctc tccgggtccg gtaaatcaac
    16261 ggtcgccggg gcgctggagg aggcgttaca taaactcagc gtcagtacgt atctgctgga
    16321 tggcgacaat gttcgccacg gattatgcag cgatctcggt tttagcgatg ccgatcgtaa
    16381 agagaatatc cgtcgcgtcg gtgaagtggc gaatttgatg gttgaagccg gactggtggt
    16441 gctgaccgca tttatctcgc cacaccgcgc cgaacgccag atggttcgcg aacgcgtagg
    16501 agaagggcgc tttattgaag tgtttgtcga tacgccgctg gcgatttgcg aagcccgcga
    16561 tccaaaaggc ttatataaga aagcgcgtgc cggtgaactg cgcaacttta cgggaataga
    16621 ttccgtttac gaagcgcctg aatcggcaga aattcatctc aatggtgaac aattagtaac
    16681 aaatttggta cagcaattat tagatctgtt gagacagaac gatattatca gatcctgaga
    16741 caccaccggg tttccatgcc cggttcgtca aagtaacagg atcaaatatg cgtaatagcc
    16801 ataacattac actaacaaat aacgacagcc ttactgagga tgaagaaact acatggtcac
    16861 tgcctggtgc cgtggtcggt tttatctcct ggttatttgc gctggcgatg ccgatgttga
    16921 tttatggctc taacacgctg ttcttctttc tctacacctg gcctttcttt ctggcactga
    16981 tgcctgtcgc ggtagtggtg gggattgcgc tgcattcatt gatggacgga aagctacgct
    17041 acagtattgt tttcaccctg gtgacagtag gcattatgtt tggcgcactg tttatgtggc
    17101 tactgggcta atttgtactt tcttgtctcc tctgttcata attcaaaccg taactaataa
    17161 tgagattatg ttctgcacgc cctgggtata cgtaacaatg ggcaaatgtg gtacatttgc
    17221 ccgcgttgtc gcggtatccc caacagagga tgtagagtcg tcttcggatg catgggatga
    17281 tgatgcagtt tttcaggggg caggatgggt aaactaacgc tgctgttgct ggctattctg
    17341 gtctggctac agtattcgct gtggttcggt aagaacggta tacatgacta tacccgcgtc
    17401 aatgatgatg tggcggcact gcaagctaca aacgcgaaac ttaaagcgcg aaacgatcaa
    17461 ctttttgccg aaattgacga tctcaatggc ggccaggagg cgctcgaaga gcgtgcgcgt
    17521 aatgaactca gcatgaccag gccgggcgaa actttttatc gtctggtgcc tgacgcgtcg
    17581 aagcgcgcac agtctgcggg gcaaaacaat cgataaatca gcccgggaat taacatggca
    17641 accactcatt tggatgtttg cgccgtggtt ccggcggccg gatttggccg tcgaatgcaa
    17701 acggaatgtc ctaagcaata tctctcaatc ggtaatcaaa ccattcttga acactcggtg
    17761 catgcgctgc tggcgcatcc ccgggtgaca cgtgtcgtca ttgccataag tcctggcgat
    17821 agccgttttg cacaacttcc tctggcgaat catccgcaaa tcaccgttgt agatggcggt
    17881 gatgagcgtg ccgattccgt gctggcaggt ctgaaagccg ctggcgatgt gcagtgggta
    17941 ttggtgcatg acgccgctcg tccttgtttg catcaggatg accttgcgcg attgttggcg
    18001 ttgagcgaaa ccagccgcac gggagggatc ctcgccgcac cagtgcgcga tactatgaaa
    18061 cgtgccgaac cgggcaaaaa tgccattgct cataccgttg atcgcaacgg cttatggcac
    18121 gcgctgacgc cgcaattttt ccctcgtgag ctgttacatg actgtctgac gcgcgctcta
    18181 aatgaaggcg ctaccattac cgacgaagcc tcggcgctgg aatattgcgg atttcatccg
    18241 cagttggtcg aaggccgcgc ggataacatt aaagtcacgc gcccggaaga tttggcactg
    18301 gccgagtttt acctcacccg aaccatccat caggagaata cataatgcga attggacacg
    18361 gttttgacgt acacgccttt ggcggtgaag gcccaattat cattggtggc gtacgcattc
    18421 cttacgaaaa aggattgctg gcgcattctg atggcgacgt ggcgcttcat gcgttgaccg
    18481 atgcattgct tggcgcggcg gcgctggggg atatcggcaa actgttcccg gataccgatc
    18541 cggcatttaa aggtgccgac agccgcgagc tgctacgcga agcctggcgt cgtattcagg
    18601 cgaagggtta tacccttggc aacgtcgatg tcactatcat cgctcaggca ccgaagatgt
    18661 tgccgcacat tccacaaatg cgcgtgttta ttgccgaaga tctcggctgc catatggatg
    18721 atgttaacgt gaaagccact actacggaaa aacttggatt taccggacgt ggggaaggga
    18781 ttgcctgtga agcggtggcg ctactcatta aggcaacaaa atgattgagt ttgataatct
    18841 cacttacctc cacggtaaac cgcaaggcac cgggctgctg aaagccaatc cggaagactt
    18901 tgtggtggtg gaagatttgg gctttgagcc tgatggtgaa ggtgagcata ttctggttag
    18961 aatcctcaaa aacggctgca atacccgttt tgtggcggat gcactggcga aattcctgaa
    19021 aattcattcc cgtgaagtca gcttcgctgg gcaaaaagac aaacatgctg taacggaaca
    19081 gtggttatgt gctcgcgtgc cgggcaagga aatgcccgat ctgagcgcct ttcaactgga
    19141 aggctgccag gtgctggagt atgcgcggca caagcgcaag ctgcgtttag gggcgctgaa
    19201 aggtaacgcc tttaccctgg ttctgcgcga agtgagcaat cgcgatgacg ttgaacaacg
    19261 tctgatcgat atttgcgtaa aaggtgtacc gaactacttc ggtgcccaac gttttgggat
    19321 tggcggtagc aacttgcagg gcgcgctgcg ctgggcgcaa accaatactc cggtgcgcga
    19381 tcgcaataaa cggagttttt ggttgtcggc agcccgcagt gcgttgttta atcagattgt
    19441 tgctgagcgc ctcaaaaaag cagacgttaa tcaagttgtt gacggcgatg cgctacaatt
    19501 agccggacgt ggtagctggt ttgtagcaac caccgaagaa ctggcggaat tacagcgtcg
    19561 cgtcaacgat aaagagctga tgataaccgc cgcattgccg ggcagtggcg aatggggaac
    19621 tcagcgtgaa gcgctggcat tcgaacaagc agctgtcgcc gcagaaactg aattacaagc
    19681 tttactggtg cgcgaaaaag ttgaagccgc gcgcagagcg atgctgctgt atccgcaaca
    19741 attaagctgg aactggtggg atgacgtcac cgtagagatc cgtttctggc ttccggcggg
    19801 tagttttgca accagcgttg tcagggaact tatcaacaca acaggtgatt atgcgcatat
    19861 tgctgagtaa ttatgacggg gtacatgcac ccggtataca aacgctggcg aaagccttgc
    19921 gtgagtttgc tgacgttcag gtggtcgccc ccgatcgtaa ccgcagcggc gcttcaaatt
    19981 ctctgacact ggaatcctcc ctgcgcacgt ttacctttga aaatggtgat attgctgtgc
    20041 aaatgggaac cccgaccgat tgtgtctatc ttggcgtgaa tgctctgatg cgtccgcgcc
    20101 cggacattgt tgtgtccgga attaacgccg ggccgaatct gggggatgat gttatttatt
    20161 ccggtacggt agccgccgcg atggaaggcc gtcatttagg ttttccggcg ctggccgtct
    20221 cgcttgacgg gcataaacat tacgacactg ccgcggcggt aacctgttca attttgcgcg
    20281 cactgtgtaa agagccgctg cgcaccggac gtattcttaa tattaacgtt caggatttac
    20341 ccttggatca aatcaaaggt attcgcgtga cgcgctgcgg tacacgacat ccggcagatc
    20401 aggtgatccc gcagcaagat ccgcgcggca atacgctgta ctggattggc ccgccgggcg
    20461 gtaaatgtga tgccggtccg gggaccgatt ttgctgcggt agatgagggc tatgtctcca
    20521 tcacgccgct gcatgtggat ttaactgcgc atagcgcgca agatgtggtt tcagactggt
    20581 taaacagcgt gggagttggc acgcaatggt aagcagacgc gtacaagcac ttctggatca
    20641 attacgtgcg caaggtattc aggatgagca ggtgctgaat gcacttgccg ccgtgccgcg
    20701 tgaaaaattc gttgatgaag cgtttgaaca aaaagcctgg gacaatatcg ccttgccgat
    20761 aggtcagggg cagacaattt cgcagccgta tatggtggcg cgaatgaccg aattactcga
    20821 gctgacgccg cagtcgcggg tgctggaaat tggcactggt tcgggatatc aaacggcaat
    20881 cctggcgcat cttgtccagc atgtttgctc ggttgaacgg attaaaggct tgcagtggca
    20941 ggcacgtcgc cgcctgaaaa atcttgattt acataatgtt tcaacccgtc atggtgatgg
    21001 atggcaaggt tggcaggcac gtgcgccgtt tgacgctatc attgttacgg cggcaccgcc
    21061 ggaaattcca actgcgctaa tgacgcagct ggacgaaggc gggattctcg tcttacccgt
    21121 aggggaggag caccagtatt tgaaacgggt gcgtcgtcgg ggaggcgaat ttattatcga
    21181 taccgtggag gccgtgcgct ttgtcccttt agtgaagggt gagctggctt aaaacgtgag
    21241 gaaatacctg gatttttcct ggttattttg ccgtaggtca gcgtatcgtg aacatctttt
    21301 ccagtgttca gtagggtgcc ttgcacggta attatgtcac tggttattaa ccaatttttc
    21361 ctgggggata aatgagcgcg ggaagcccaa aattcaccgt tcgccgcatt gcggctttgt
    21421 cactggtttc gctatggctg gcaggctgtt ctgacacttc aaatccaccg gcaccggtca
    21481 gctccgttaa tggcaatgcg cctgcaaata ctaattctgg tatgttgatt acgccgccgc
    21541 cgaaaatggg gacgacgtct acagcgcagc aaccgcaaat tcagccggta cagcagccac
    21601 aaattcaggc tactcaacaa ccgcaaatcc agccagtgca gccagtagct cagcagccgg
    21661 tacagatgga aaacggacgc atcgtctata accgtcagta tgggaacatt ccgaaaggca
    21721 gttatagcgg cagtacctat accgtgaaaa aaggcgacac acttttctat atcgcctgga
    21781 ttactggcaa cgatttccgt gaccttgctc agcgcaacaa tattcaggca ccatacgcgc
    21841 tgaacgttgg tcagaccttg caggtgggta atgcttccgg tacgccaatc actggcggaa
    21901 atgccattac ccaggccgac gcagcagagc aaggagttgt gatcaagcct gcacaaaatt
    21961 ccaccgttgc tgttgcgtcg caaccgacaa ttacgtattc tgagtcttcg ggtgaacaga
    22021 gtgctaacaa aatgttgccg aacaacaagc caactgcgac cacggtcaca gcgcctgtaa
    22081 cggtaccaac agcaagcaca accgagccga ctgtcagcag tacatcaacc agtacgccta
    22141 tctccacctg gcgctggccg actgagggca aagtgatcga aacctttggc gcttctgagg
    22201 ggggcaacaa ggggattgat atcgcaggca gcaaaggaca ggcaattatc gcgaccgcag
    22261 atggccgcgt tgtttatgct ggtaacgcgc tgcgcggcta cggtaatctg attatcatca
    22321 aacataatga tgattacctg agtgcctacg cccataacga cacaatgctg gtccgggaac
    22381 aacaagaagt gaaggcgggg caaaaaatag caaccatggg tagcaccgga accagttcaa
    22441 cacgcttgca ttttgaaatt cgttacaagg ggaaatccgt aaacccgctg cgttatttgc
    22501 cgcagcgata aatcggcgga accaggcttt tgcttgaatg ttccgtcaag ggatcacggg
    22561 taggagccac cttatgagtc agaatacgct gaaagttcat gatttaaatg aagatgcgga
    22621 atttgatgag aacggagttg aggtttttga cgaaaaggcc ttagtagaag aggaacccag
    22681 tgataacgat ttggccgaag aggaactgtt atcgcaggga gccacacagc gtgtgttgga
    22741 cgcgactcag ctttaccttg gtgagattgg ttattcacca ctgttaacgg ccgaagaaga
    22801 agtttatttt gcgcgtcgcg cactgcgtgg agatgtcgcc tctcgccgcc ggatgatcga
    22861 gagtaacttg cgtctggtgg taaaaattgc ccgccgttat ggcaatcgtg gtctggcgtt
    22921 gctggacctt atcgaagagg gcaacttggg gctgatccgc gcggtagaga agtttgaccc
    22981 ggaacgtggt ttccgcttct caacatacgc aacctggtgg attcgccaga cgattgaacg
    23041 ggcgattatg aaccaaaccc gtactattcg tttgccgatt cacatcgtaa aggagctgaa
    23101 cgtttacctg cgaaccgcac gtgagttgtc ccataagctg gaccatgaac caagtgcgga
    23161 agagatcgca gagcaactgg ataagccagt tgatgacgtc agccgtatgc ttcgtcttaa
    23221 cgagcgcatt acctcggtag acaccccgct gggtggtgat tccgaaaaag cgttgctgga
    23281 catcctggcc gatgaaaaag agaacggtcc ggaagatacc acgcaagatg acgatatgaa
    23341 gcagagcatc gtcaaatggc tgttcgagct gaacgccaaa cagcgtgaag tactggcacg
    23401 tcgattcggt ttgctggggt atgaagcggc aacactggaa gatgtaggtc gtgaaattgg
    23461 cctcacccgt gaacgtgttc gccagattca ggttgaaggc ctgcgccgtt tgcgcgaaat
    23521 cctgcaaacg caggggctga atatcgaagc gctgttccgc aagtaagtaa gcatctgtca
    23581 gaaaggccag tctcaagcga ggctggcctt tttcttttgt ttgggaagct tgtgtttttg
    23641 tcgcctggat aagacacgtc agcgtcctac ccggcaatca gcattctatg tgctcatcat
    23701 cttgcgcacc aactgcataa acagctcctg ctcttctcca ctcaaacgcc ccaaaaattc
    23761 ctcatccacg ctgtcaccaa taggtatcgc cgcagcaagt agcttttctc cttcggcagt
    23821 cagccagaca aagcgtcgcc gcttgtcagc gacgtcatgt tcacgtctga ctaagccacg
    23881 attttccatt cttgccaaca tttctgctag cgttgctttg gtgctgactg ctgcttctat
    23941 cagtgccacc tgttcaatac caggtttatc agcaatggcg cgcatcaccg cgtactgtgg
    24001 tttggtcagg tcaggtaact cgtgctgcca gcgagccgtg tgctgctgaa aaagctgtcg
    24061 taactgatgg aacgctttat ttcgtaacgc catgtaaact ccgggctaat tctctgtcgc
    24121 tatcatagcg gttttttagt gacgaagaag atgttgttac ttttcaatcc ttgccgctgt
    24181 ggaaatgaag gagtatgtta ataataacgt tcgtatacga ataattaaga gaataagcaa
    24241 tgaaactgat cgtcgggatg acaggggcta ccggtgcgcc tcttggtgtg gcattactgc
    24301 aagcgctgcg tgagatgccg aatgtcgaga ctcatctggt gatgtcgaag tgggcgaaaa
    24361 ccaccattga actggaaacg ccttacagcg ctcgcgatgt tgctgccctc gcagacttca
    24421 gccataaccc ggcggatcag gcggcgatca tctcatccgg ttcttttcgt accgacggca
    24481 tgatcgttat tccgtgcagt atgaaaacgc tcgccggtat ccgcgctggt tacgccgatg
    24541 gcctggtagg gcgcgcggcg gacgtcgtgc tcaaagaagg ccgcaaactg gtgctggtgc
    24601 cgcgtgaaat gccgcttagc accatccatc tcgaaaatat gctcgcactt tcacgcatgg
    24661 gcgtggcgat ggtgccgccg atgcctgcct tttataacca tcccgaaacg gtagatgaca
    24721 ttgtccacca tgtggtagcc cgcgtgctgg atcaatttgg cctcgaacat ccccacgcca
    24781 ggcgctggca aggattgccg caggcccgga atttttctca ggagaatgaa taatggcatt
    24841 tgatgattta cgcagctttt tacaggcgct tgatgaccac ggccagttac tgaaaatcag
    24901 cgaagaagtg aacgctgagc cggatctggc agcagcagct aacgccaccg ggcgtatcgg
    24961 cgacggcgcg cccgcgctgt ggtttgataa tattcgcggc tttaccgatg cccgcgtggc
    25021 gatgaatacc atcggttcct ggcagaacca cgcgatttcc cttggcctgc caccaaacac
    25081 cccggtcaaa aagcagattg atgagtttat ccgccgctgg gataacttcc cgattgcccc
    25141 ggagcgccgc gccaatccag cctgggcgca gaacaccgtc gatggcgaag agatcaacct
    25201 gttcgatatt ctgccgctgt ttcgcctgaa tgatggcgat ggcggtttct atctcgacaa
    25261 agcgtgcgtg gtttcccgcg atccgctcga cccggataac ttcggcaagc agaacgtcgg
    25321 catctaccgc atggaagtga agggcaagcg taagctcggc ctgcaaccgg tgccgatgca
    25381 cgatatcgcc ctgcatctgc ataaagcaga agagcgcggt gaagatctgc cgattgcgat
    25441 cacgctcggt aacgatccga tcatcacgct gatgggggcc acgccgctga aatacgatca
    25501 gtctgagtat gaaatggcag gcgcgctgcg tgaaagcccg tacccgatcg ccaccgctcc
    25561 gctgaccggt tttgatgtgc cgtggggttc agaagtgatc ctcgaagggg ttatcgaaag
    25621 ccgtaaacgc gaaatcgaag ggccgttcgg tgagtttacc gggcactact ccggtgggcg
    25681 caacatgacc gtggtgcgca tcgataaagt ctcttatcgc agcaaaccga ttttcgaatc
    25741 actctatctc ggtatgccgt ggaccgaaat cgactaccta tgactaggta atttattcaa
    25801 gataaccggg aagagattaa aaaagacggg aatagattgg aatcaacaag ttaggaactc
    25861 aaggttttca agataaagcg agtccaaaaa tgttaaacgg ttcgatatca ataaaacatt
    25921 caagataact cacaggttct tacaatagcc tttaaagacc gttcaaaaac gattcaggtg
    25981 tttattatag cgctgatggg tgcaagcgcc aaaaccaatg taattttagc acagctaggc
    26041 acttgcactg ggttatgcgc tggctgcttt ctgaggtaga tttaagtgat cgcccttagc
    26101 ttcggacaac gcgtaaatct ctctaacctt ttcgtctggc aaattaccaa ggcttaacgc
    26161 tatggctcta cgaactccaa ctagagcgtg caactcttgg atgtgctcat ccagaagaat
    26221 gagtaattgc tccgctcctt ttctcccaag aacatcagct acttgttgtt tttcttgtgg
    26281 tttaagaaat gacaagaaca ttagaatttg agcatcaaca ttatccgcat tctctttttt
    26341 gggttctttg gaatctttta gttcaacatc accctctccc gtcatgagcc attccaaggt
    26401 aacattttcg ctttctgcta tcttttttaa ctgatacggg cgtggtatcg gtgcgtaatc
    26461 ccgcctgttg taataatttt ttaaagtgcc ttcattgatc ccccatgccc gagcagccgc
    26521 atttcttgaa gggtatcgtt ctactaacct aaagattctt tcgataatcg gttcttttcc
    26581 atcggcagga aaagaacctt ggggtgcttt tctcattctt ggttcctttc tatctaatcc
    26641 attgataaat aataaaatta aaaaataaac cataaaaaca ccaaagaacc ctttgcaggg
    26701 aatctttggt atcttaatct ttatccatac ggatatccga ccggatatcc gcgcgggtaa
    26761 ttttgtaggt taaaggaaag atgagacaaa gagaagatag tgaccgcgat tggccttcgc
    26821 tgaagattca gctggcgctc aaagataacg ggtggtcttc cttccgggag ctttcactcc
    26881 atgctggtta tgctcaggat tcactgaaag cggtagttcg cacaccaaac aaaaaatacg
    26941 aacgcgtaat ttctgacgct ataggtgtac cgccagaaat catctggccc agccggtatc
    27001 gtaacgatgg atttatcagg aaggcgtcgt gatatgcagt cgtacgttac cgtaaatgac
    27061 ctgctcggcg taccaggaat gccagccacc acaaaaggca tccgacaggc gttacagcgt
    27121 ttttctcgtg atcttggtga cgttagtcgc cgtcgggaag gcacaaaagc cattgagtac
    27181 cacattgatt gtctgcctga aataacacga aaggcactgc gtgaacgcta tgtggaacag
    27241 ctggtggcta cagagaacaa tgtttctgaa gtgaaagctg ttaccagaaa aacacgcaat
    27301 cctgacgctg tccaggcaat cgaagcatat cgcggttctc cacaactgat ggaagaacgc
    27361 ctgaatgcgc tgaccgaaaa ccagcgctgg gtatctgaag caagagctgc gctggtggtg
    27421 gaagtgctga agctggaaag cgccggtaac cccgggcgac tgaaagccat taactttctt
    27481 gttgaaaaag cccgtaaagg tgagctgccg gagcgcctgc aacaggccgc agttaacgcc
    27541 aatgcaaaac gtggcgctaa tcgtactatc agccgcgatc cactttacca gtgggttctg
    27601 aaatacaacc agtcgcagaa cgccgccgaa cggctgctgt tactggcacc gggaaaacgc
    27661 gacgaaataa aaccggaaga aatcagctgg ctgcctgaat ttctggcgca gtatcgccag
    27721 gtgaacggca gacccatgtc agaagcctac gaggattttg tcgcagaatg gcagcgtcgc
    27781 catgcggacg aaccgtacat gctggaggtg atgccttcct atgatgttgt ccggtatgcc
    27841 atgaaaaaac tgccggaagt ggtgaaacaa aaagggcgcg ttaccggcag tgaataccgt
    27901 cagcttgaag gattcacccg ccgcgactgg acagctatgc cggtgaatta tgtctggatt
    27961 ggtgacggtc acggtatgaa gctgaaatgt gcgcatccga tccacgggcg tccattctca
    28021 ccggaagtca cgtttgtgat cgatggtgga acacggtttg ttgttggctg gagtcttgac
    28081 cttgccgaaa acgtgttcgc cgttgccggt gcgatacagc acggcattcg caaccacgga
    28141 aaaccgttcc tgtattactc ggataacggt tccggtgaaa cggctgacat gctggacaaa
    28201 gaggtcgtgg gtattctgcc acgcctgggg attaaacacc ctaccggtat tgccggtaat
    28261 ccacagggac gcggcattat tgaacgcctg aaccggacac tgccgatgcg tatagccagg
    28321 aggtatcgca cctattttgg caagggtgct gaccgggaat cgttgcgtgt acttaaccgt
    28381 gatctgcgtt cagcgtttaa cgccctgcaa caggacaagc ctctgaacga ccgccagaaa
    28441 gcggcaatgc gcgagctgcc atcgtgggcg gagcttattg aggctattcg tgaaggcgtg
    28501 gaatggtaca acaaccgccc gcattctgag ctgccgatga aaccgaacgg acggcattac
    28561 agcccgacag agttcagaaa aaaacgccag gcagaagaag ac
//
LOCUS       DAJTYM010000049        28355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTYM010000049 DAJTYM010000000
VERSION     DAJTYM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(31..1026)
                     /gene="yjfF"
                     /locus_tag="OO575_002155"
                     /pseudo
     CDS             complement(31..1026)
                     /gene="yjfF"
                     /locus_tag="OO575_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1013..2035)
                     /locus_tag="OO575_002156"
     CDS             complement(1013..2035)
                     /locus_tag="OO575_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6665112.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2049..3551)
                     /locus_tag="OO575_002157"
     CDS             complement(2049..3551)
                     /locus_tag="OO575_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6665113.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3691..4647)
                     /gene="ytfQ"
                     /locus_tag="OO575_002158"
                     /pseudo
     CDS             complement(3691..4647)
                     /gene="ytfQ"
                     /locus_tag="OO575_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4957..5487
                     /gene="ppa"
                     /locus_tag="OO575_002159"
     CDS             4957..5487
                     /gene="ppa"
                     /locus_tag="OO575_002159"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6665114.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5867..6208)
                     /locus_tag="OO575_002160"
     CDS             complement(5867..6208)
                     /locus_tag="OO575_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6665115.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6211..9990)
                     /gene="tamB"
                     /locus_tag="OO575_002161"
     CDS             complement(6211..9990)
                     /gene="tamB"
                     /locus_tag="OO575_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6665116.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9987..11720)
                     /gene="tamA"
                     /locus_tag="OO575_002162"
     CDS             complement(9987..11720)
                     /gene="tamA"
                     /locus_tag="OO575_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6665117.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11926..12564
                     /gene="msrA"
                     /locus_tag="OO575_002163"
     CDS             11926..12564
                     /gene="msrA"
                     /locus_tag="OO575_002163"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6665118.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12887..14230
                     /locus_tag="OO575_002164"
     CDS             12887..14230
                     /locus_tag="OO575_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6665119.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14292..14498)
                     /locus_tag="OO575_002165"
     CDS             complement(14292..14498)
                     /locus_tag="OO575_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6665120.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14823..15377
                     /locus_tag="OO575_002166"
     CDS             14823..15377
                     /locus_tag="OO575_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6665121.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15440..16378)
                     /locus_tag="OO575_002167"
     CDS             complement(15440..16378)
                     /locus_tag="OO575_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665122.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16590..17330)
                     /gene="cysQ"
                     /locus_tag="OO575_002168"
     CDS             complement(16590..17330)
                     /gene="cysQ"
                     /locus_tag="OO575_002168"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6665123.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17520..19463
                     /gene="cpdB"
                     /locus_tag="OO575_002169"
     CDS             17520..19463
                     /gene="cpdB"
                     /locus_tag="OO575_002169"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6665124.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19589..19969)
                     /locus_tag="OO575_002170"
     CDS             complement(19589..19969)
                     /locus_tag="OO575_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6665125.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20058..20918
                     /gene="qorB"
                     /locus_tag="OO575_002171"
     CDS             20058..20918
                     /gene="qorB"
                     /locus_tag="OO575_002171"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6665126.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21027..21992
                     /locus_tag="OO575_002172"
     CDS             21027..21992
                     /locus_tag="OO575_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6665127.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22100..22762
                     /gene="ytfE"
                     /locus_tag="OO575_002173"
     CDS             22100..22762
                     /gene="ytfE"
                     /locus_tag="OO575_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6665128.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22807..24219)
                     /gene="cycA"
                     /locus_tag="OO575_002174"
                     /gene_synonym="dagA"
     CDS             complement(22807..24219)
                     /gene="cycA"
                     /locus_tag="OO575_002174"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6665129.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24527..25147)
                     /gene="fklB"
                     /locus_tag="OO575_002175"
     CDS             complement(24527..25147)
                     /gene="fklB"
                     /locus_tag="OO575_002175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6665130.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25366..25911
                     /locus_tag="OO575_002176"
     CDS             25366..25911
                     /locus_tag="OO575_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR6665131.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25895..26542)
                     /locus_tag="OO575_002177"
     CDS             complement(25895..26542)
                     /locus_tag="OO575_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6665132.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26754..27545
                     /locus_tag="OO575_002178"
     CDS             26754..27545
                     /locus_tag="OO575_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR6665133.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27555..>28355
                     /locus_tag="OO575_002179"
     CDS             27555..>28355
                     /locus_tag="OO575_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6665134.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT         6957 a         7549 c         6992 g         6857 t
ORIGIN      
        1 atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga ctcttgtcac
       61 tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg ctataaaaat
      121 aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc cgtcaaagtt
      181 gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa gcaccgtccc
      241 gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt ccagttccac
      301 acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa tccccgccag
      361 tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag tagaaatccc
      421 catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc caaaacgggt
      481 acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc ccatcgcact
      541 aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat aaatgggatg
      601 gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa agaacatccc
      661 cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc ccataaatgc
      721 gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg agaggccgaa
      781 atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac cgacggagag
      841 atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc caagaaaggc
      901 gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa actgggtcag
      961 gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca agttacgttt
     1021 tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga acaatcagca
     1081 cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag cccgaaagca
     1141 aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc agcaggttaa
     1201 agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg tccagctcca
     1261 gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg ataatgcccg
     1321 caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt cgcgtgttta
     1381 ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac atccccagcg
     1441 ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata atgaccggcg
     1501 ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag ttaaacgtga
     1561 cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc aggatcaggg
     1621 tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac aatcccgcca
     1681 ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc gcgaccgtca
     1741 tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga tcgataccac
     1801 cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct gcacggttaa
     1861 gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc cagaaatgcg
     1921 gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc ggcatccccg
     1981 tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga ggcatcacac
     2041 tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg tgccagcggg
     2101 atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata gcccaccagc
     2161 tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag cgtttcaatc
     2221 aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc atcaaggatc
     2281 agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg attgccaccg
     2341 gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg gcggataaag
     2401 cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc gcgctgggcc
     2461 tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc atcggttttc
     2521 ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga tcgcagggtt
     2581 tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc gaagatcact
     2641 tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat ctcgccgggg
     2701 cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt gaacgcggca
     2761 acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt atccagctcg
     2821 cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc ccgacagcct
     2881 acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac ctgatcgaga
     2941 aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat caggccaaat
     3001 aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat cagcactttg
     3061 gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac cgaaaaacgg
     3121 ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc ggtggcgcgc
     3181 ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc tatgaataga
     3241 ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt gccgataccg
     3301 agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag ccagatggtg
     3361 ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt tcccgccccg
     3421 ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac gttgtctaac
     3481 gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc ctggtgttgg
     3541 tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga taagacgcgg
     3601 taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg ccttatccgg
     3661 cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct tcttctctaa
     3721 ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca gcttttcagg
     3781 catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg ccatgttcgg
     3841 cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga tgtccggtac
     3901 accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt ctttaattgc
     3961 ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga tgtttttgcc
     4021 gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc gggtgaagtc
     4081 acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt cggcaaagcc
     4141 tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca ccacgttgca
     4201 tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt cgaggatgtt
     4261 gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg aacggtcgag
     4321 caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc aacccgtcgc
     4381 aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac gtaccgcttt
     4441 aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc cgcgtttttc
     4501 ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg atccgacctg
     4561 cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg ccgagactgc
     4621 agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat tattattggt
     4681 gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg cgttacatca
     4741 cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc atctcactat
     4801 gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa aactggcgaa
     4861 aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct ataatacttg
     4921 gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa cgtccctgcg
     4981 ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa cgcagatccg
     5041 atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt catgtccacc
     5101 gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct ggacggtgac
     5161 ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt gatccgttgc
     5221 cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa actggttgcg
     5281 gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa cgatctgcct
     5341 gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct cgaaaaaggc
     5401 aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga aatcgttgct
     5461 tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg aacgccgggc
     5521 ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc caccatcatt
     5581 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
     5641 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
     5701 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
     5761 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
     5821 tacgtcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac ttatccctgt
     5881 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
     5941 tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
     6001 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
     6061 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
     6121 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
     6181 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
     6241 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
     6301 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
     6361 tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt cgagcgctaa
     6421 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
     6481 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
     6541 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
     6601 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat cgtcttctgt
     6661 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
     6721 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
     6781 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
     6841 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
     6901 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
     6961 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
     7021 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
     7081 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
     7141 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
     7201 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
     7261 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
     7321 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
     7381 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
     7441 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
     7501 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
     7561 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
     7621 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
     7681 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
     7741 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
     7801 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
     7861 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
     7921 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc gggtattgct
     7981 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
     8041 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
     8101 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
     8161 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
     8221 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
     8281 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
     8341 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
     8401 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
     8461 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
     8521 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
     8581 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
     8641 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
     8701 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
     8761 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
     8821 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
     8881 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
     8941 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
     9001 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
     9061 acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg gccagttgtc
     9121 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
     9181 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
     9241 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
     9301 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
     9361 ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga ccacttcttc
     9421 ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
     9481 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
     9541 ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg tgatgggata
     9601 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
     9661 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
     9721 gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg cttttaccgc
     9781 cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
     9841 cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
     9901 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
     9961 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    10021 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    10081 ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg gcgaatatcg
    10141 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    10201 tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc gttggcgtat
    10261 ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc gacgaagaaa
    10321 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    10381 gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc ctggaaaacg
    10441 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    10501 tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    10561 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    10621 aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc caccagcgta
    10681 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    10741 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag ggtctgttcc
    10801 ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat ccacggcttt
    10861 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    10921 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    10981 ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga aagtcggcgg
    11041 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    11101 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    11161 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    11221 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    11281 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    11341 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    11401 ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    11461 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    11521 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    11581 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    11641 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    11701 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc cctggaacgc
    11761 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    11821 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    11881 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    11941 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    12001 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    12061 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    12121 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    12181 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    12241 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    12301 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    12361 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    12421 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    12481 gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg cggaattggc
    12541 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    12601 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgcccaaatt
    12661 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    12721 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    12781 actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    12841 atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt taaacagtat
    12901 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    12961 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    13021 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    13081 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    13141 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    13201 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    13261 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    13321 cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    13381 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    13441 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    13501 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    13561 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    13621 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    13681 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    13741 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    13801 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    13861 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    13921 tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg acgacgtcat
    13981 gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca tcggcggctt
    14041 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    14101 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    14161 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    14221 cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct tattactgca
    14281 tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt aacttcggac
    14341 atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc cttatcaaat
    14401 tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt tacgtacttc
    14461 gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg ctgagtatta
    14521 cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta atgcttagtg
    14581 actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct aaaggttaca
    14641 attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt catttttcat
    14701 atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg ataaatagca
    14761 catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa gaaggaaaca
    14821 ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg atggcttccg
    14881 cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat cggggcgagt
    14941 tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag ttagtgggaa
    15001 aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa aacgcgacgc
    15061 tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc accaccattg
    15121 ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt ctggagagta
    15181 ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc gcgcgcggtg
    15241 cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac ggtcgcgtgc
    15301 aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg gacctgctgc
    15361 aaaagttgct caaataatgg caataacata aattctggaa aaataatttt cccctatcgt
    15421 tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat actggtaacg
    15481 ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa atcttttctt
    15541 tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact tgttcggcta
    15601 attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag taatcttcat
    15661 cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag attttaagcg
    15721 caaagcccaa agattctatg actatggcaa aattatcatc aattattttt tcgtaaatat
    15781 accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat ttaccccgtt
    15841 ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca ccaaacgcct
    15901 ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc aaaatatcac
    15961 aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa tccatgaatg
    16021 agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg cctcgcaatg
    16081 ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa ttatccatgg
    16141 cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca atctgtgttg
    16201 cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc caatcttttt
    16261 tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt gtatgcgatg
    16321 actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg ggtaacatat
    16381 ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt atagaagtaa
    16441 actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt tttgcacttt
    16501 aaatacaatc tctttttatt tataactttt taggttggga taaatcagga tggcagaatc
    16561 aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa ccccggattc
    16621 aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg aacgtgcgct
    16681 ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt tggtccgaag
    16741 cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga agagccgatg
    16801 gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc atctgcatgg
    16861 gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag cttgcgcaca
    16921 ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt cattaccggc
    16981 gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt aacggtgaat
    17041 tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag ccagtaacgc
    17101 tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac cggaatatcc
    17161 ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc cgctgccgtt
    17221 accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc gtagacctgc
    17281 ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat ttctccacct
    17341 cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta atgaatgcgc
    17401 caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc actgttctat
    17461 aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg atgtccctga
    17521 tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg aatgcagcga
    17581 cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg gatttcgatt
    17641 attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc ctgattaacg
    17701 atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg attcagggga
    17761 gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt catccggttt
    17821 ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat gaatttaact
    17881 acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat gtaaatgcca
    17941 acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt aaagataccg
    18001 aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt ggcgtcgtgc
    18061 cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg gtgaatgata
    18121 ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc gatgtcattg
    18181 tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg gaaaactcag
    18241 tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat gctcacgccg
    18301 ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa ggcacgctga
    18361 atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg gtcgacttac
    18421 aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa gcacggccga
    18481 tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg gtagaaacac
    18541 tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt aaatctgccg
    18601 acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa gtcgtgaaca
    18661 acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg gcaaaactgc
    18721 cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac ccggcaagct
    18781 atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt tatctctacc
    18841 ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg ctggagtgct
    18901 ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca ctcatcaact
    18961 gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat cagattgatg
    19021 ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat gcagagcgga
    19081 ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc ctggttgcca
    19141 ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc catatcgctt
    19201 ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat gagtcgaaac
    19261 gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg atagctgccg
    19321 ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca gcgtttatta
    19381 aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg tttgcgattt
    19441 atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca accgcatccg
    19501 gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc atgaaccgta
    19561 gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc accgccaaca
    19621 cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg ctcacctgct
    19681 cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac gcgatacggt
    19741 taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt tcactcaccc
    19801 caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc gccaccagaa
    19861 tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg cactgttccg
    19921 caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt tcatacttac
    19981 ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa cacaagacaa
    20041 aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt ggtcactata
    20101 ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc gttcgtaatc
    20161 cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct gactacagcg
    20221 atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg atctcttcca
    20281 gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca aaggcggctg
    20341 gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg ctcggcctcg
    20401 ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt tacaccctgc
    20461 tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca ctggaacacg
    20521 gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg gcagattatg
    20581 cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt tacgaactgg
    20641 cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa cagagcggca
    20701 aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg aaaagcgtcg
    20761 gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca tcgaaaggtg
    20821 gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg acaacgttag
    20881 ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg gcatcctccc
    20941 gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc tgaacgtttc
    21001 ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc ctgttagcag
    21061 ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac ccggcgatgt
    21121 tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt gcctggctgg
    21181 gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg atgctcacta
    21241 tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt actggcgcgc
    21301 ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt gccaatctgc
    21361 tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca ctgatttgta
    21421 ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc cccgattttg
    21481 actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc tgctgggcat
    21541 ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat ccgatgatgt
    21601 gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat ctcgtcgcct
    21661 gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc cgtccgctgg
    21721 tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc gcactctgct
    21781 ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt gttttcgaaa
    21841 cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg ctaatgacgc
    21901 tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca aaaccagaaa
    21961 aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta aataagcctc
    22021 tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat cttatcttat
    22081 taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac tggcgctctc
    22141 tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct gtggcggtaa
    22201 gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca ttgaagctga
    22261 actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc cgctggcaga
    22321 aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc tgccggagct
    22381 gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg tgccaaaagg
    22441 gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga tgaaagaaga
    22501 gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg ggccaatcag
    22561 cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca aacacactac
    22621 caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt ataacggcat
    22681 taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg tactgttccc
    22741 acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc cagcatgata
    22801 atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc agcccaaccc
    22861 cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat cttccagtgt
    22921 cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca gcttgccgag
    22981 cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt acaccaggta
    23041 agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg ttgtaatcat
    23101 cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc cgagcagaca
    23161 gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt tagcgaacgc
    23221 tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag agaagacacc
    23281 gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc tggcagccgc
    23341 aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga ctaccgaact
    23401 ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa tgatacggat
    23461 cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt ccgcagctgt
    23521 tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc cggcaaagaa
    23581 gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga atgacgcttc
    23641 cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga caatcagcga
    23701 cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga acattttcac
    23761 ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga cccagtcgga
    23821 gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg ccataccggt
    23881 tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg gccccagtaa
    23941 atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca attcccccat
    24001 tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa tgatcgacgg
    24061 cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa tggcacctcc
    24121 aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact gttcagccgg
    24181 agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc ctgtacctgt
    24241 ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat ctgtatcggg
    24301 actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat ctaatgttaa
    24361 ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc acaaaaatac
    24421 acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa gggtttattg
    24481 ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag aggatttcca
    24541 gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct gcgccgcgct
    24601 cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc ggcatcagag
    24661 tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg ggttcaccac
    24721 gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa tgaacacgaa
    24781 cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg aattgcaggc
    24841 cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat ttcacgcctt
    24901 cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg tggatttcac
    24961 gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc agcgcatcgg
    25021 caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca ctcagttgtt
    25081 gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca aaagttgggg
    25141 tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc agccacactt
    25201 ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag aaacaatagc
    25261 aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc gttagcttat
    25321 tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc cgggcgcttt
    25381 gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg cttcatggac
    25441 ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt ggtggtcgga
    25501 tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga agcgcagtta
    25561 gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac ccaaacgcaa
    25621 ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt
    25681 aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg
    25741 gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca gatggtgaaa
    25801 atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa
    25861 caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt
    25921 caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct
    25981 ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg atcaaataat
    26041 atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct ggcttaaaaa
    26101 tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga gcatggttaa
    26161 tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt tcactatgca
    26221 caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca ataattttct
    26281 caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt tgtcgaatat
    26341 cgttccaggt atattccagc acttcgataa ataaattttc tttattacca aagtggtgat
    26401 aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg gccccttcat
    26461 aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc aggctctgta
    26521 cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg actatcataa
    26581 cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat caggttatca
    26641 aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt ttatcctcca
    26701 gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat tgtatgaaat
    26761 ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag ggctgtgcgc
    26821 aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat gccgacagcg
    26881 tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa gtgatttgct
    26941 tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg gcgcaaaacc
    27001 agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa aaacgcggtg
    27061 acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac acccgcgcgc
    27121 cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa ccggaagcgg
    27181 agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg ctggcgatga
    27241 atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt ctgtttgctg
    27301 ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc atcaaaactg
    27361 atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt ctggtgccgt
    27421 ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg gcggaaggta
    27481 aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt atgccgcgct
    27541 tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt atcaataccg
    27601 cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc gcacgcggta
    27661 ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag caaatcaccc
    27721 gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact tactacaccg
    27781 cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg ctggacgacg
    27841 cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca acgggcacta
    27901 aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg ctgccacatt
    27961 cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca gctgccgatc
    28021 gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc gatcccgatg
    28081 gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat ccggatctcg
    28141 ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc gactggttaa
    28201 tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc atcaatattc
    28261 cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa ctggaaatat
    28321 tctcgcctta ctggtggcag aaagatatta acagc
//
LOCUS       DAJTYM010000050        28169 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTYM010000050 DAJTYM010000000
VERSION     DAJTYM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..496
                     /locus_tag="OO575_002180"
     CDS             <1..496
                     /locus_tag="OO575_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665135.1"
                     /translation="AIFMRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRI
                     ISASQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIP
                     HEAGHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLL
                     NVTK"
     gene            493..879
                     /locus_tag="OO575_002181"
     CDS             493..879
                     /locus_tag="OO575_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665136.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            904..3117
                     /gene="tssI"
                     /locus_tag="OO575_002182"
     CDS             904..3117
                     /gene="tssI"
                     /locus_tag="OO575_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6665137.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3140..3585
                     /locus_tag="OO575_002183"
                     /pseudo
     CDS             3140..3585
                     /locus_tag="OO575_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3607..4014
                     /locus_tag="OO575_002184"
     CDS             3607..4014
                     /locus_tag="OO575_002184"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR6665138.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4011..6299
                     /locus_tag="OO575_002185"
     CDS             4011..6299
                     /locus_tag="OO575_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6665139.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6296..7285
                     /locus_tag="OO575_002186"
     CDS             6296..7285
                     /locus_tag="OO575_002186"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665140.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7385..7588
                     /locus_tag="OO575_002187"
     CDS             7385..7588
                     /locus_tag="OO575_002187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665141.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7606..7926
                     /locus_tag="OO575_002188"
     CDS             7606..7926
                     /locus_tag="OO575_002188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665142.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(7992..8224)
                     /locus_tag="OO575_002189"
                     /pseudo
     CDS             complement(7992..8224)
                     /locus_tag="OO575_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8334..9104)
                     /gene="yafV"
                     /locus_tag="OO575_002190"
     CDS             complement(8334..9104)
                     /gene="yafV"
                     /locus_tag="OO575_002190"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6665143.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9285..9731
                     /gene="ivy"
                     /locus_tag="OO575_002191"
     CDS             9285..9731
                     /gene="ivy"
                     /locus_tag="OO575_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6665144.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9774..12218)
                     /gene="fadE"
                     /locus_tag="OO575_002192"
     CDS             complement(9774..12218)
                     /gene="fadE"
                     /locus_tag="OO575_002192"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6665145.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12458..13036
                     /gene="lpcA"
                     /locus_tag="OO575_002193"
     CDS             12458..13036
                     /gene="lpcA"
                     /locus_tag="OO575_002193"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6665146.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13242..14009
                     /locus_tag="OO575_002194"
     CDS             13242..14009
                     /locus_tag="OO575_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6665147.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13980..14720)
                     /locus_tag="OO575_002195"
     CDS             complement(13980..14720)
                     /locus_tag="OO575_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6665148.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15104..15625
                     /locus_tag="OO575_002196"
                     /pseudo
     CDS             15104..15625
                     /locus_tag="OO575_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15801..16298
                     /gene="rayT"
                     /locus_tag="OO575_002197"
     CDS             15801..16298
                     /gene="rayT"
                     /locus_tag="OO575_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6665149.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16515..18227)
                     /locus_tag="OO575_002198"
                     /pseudo
     CDS             complement(16515..18227)
                     /locus_tag="OO575_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18199..18984
                     /gene="lafU"
                     /locus_tag="OO575_002199"
     CDS             18199..18984
                     /gene="lafU"
                     /locus_tag="OO575_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6665150.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19055..20110
                     /gene="dinB"
                     /locus_tag="OO575_002200"
     CDS             19055..20110
                     /gene="dinB"
                     /locus_tag="OO575_002200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6665151.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20162..20455
                     /gene="yafN"
                     /locus_tag="OO575_002201"
     CDS             20162..20455
                     /gene="yafN"
                     /locus_tag="OO575_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6665152.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20458..20856
                     /gene="yafO"
                     /locus_tag="OO575_002202"
     CDS             20458..20856
                     /gene="yafO"
                     /locus_tag="OO575_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6665153.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            20866..21318
                     /locus_tag="OO575_002203"
     CDS             20866..21318
                     /locus_tag="OO575_002203"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6665154.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21564..22607
                     /locus_tag="OO575_002204"
     CDS             21564..22607
                     /locus_tag="OO575_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6665155.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22669..23283
                     /locus_tag="OO575_002205"
                     /pseudo
     CDS             22669..23283
                     /locus_tag="OO575_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23340..24797)
                     /gene="pepD"
                     /locus_tag="OO575_002206"
     CDS             complement(23340..24797)
                     /gene="pepD"
                     /locus_tag="OO575_002206"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6665156.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25059..25517
                     /gene="gpt"
                     /locus_tag="OO575_002207"
     CDS             25059..25517
                     /gene="gpt"
                     /locus_tag="OO575_002207"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6665157.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25609..26853
                     /gene="frsA"
                     /locus_tag="OO575_002208"
     CDS             25609..26853
                     /gene="frsA"
                     /locus_tag="OO575_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6665158.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            26911..27312
                     /gene="crl"
                     /locus_tag="OO575_002209"
     CDS             26911..27312
                     /gene="crl"
                     /locus_tag="OO575_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6665159.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27351..>28169)
                     /gene="phoE"
                     /locus_tag="OO575_002210"
     CDS             complement(27351..>28169)
                     /gene="phoE"
                     /locus_tag="OO575_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6665160.1"
                     /translation="EFAGNKAESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALYDVE
                     AWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDV
                     KKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDA
                     NNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGI
                     GDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDTVAVGMTYQF"
BASE COUNT         6924 a         7030 c         7514 g         6701 t
ORIGIN      
        1 agctattttt atgcgagcga tatatttatc tgttcagcag gcatggaatg ggaaaataac
       61 ttattctgtt tccggtgaat ctgagttcgc taaaaaattt cagggtaaag ctttgccttt
      121 tgatgtaaga ataatttctg catctcagaa tgaagactgg ttggttattg caacaaaggt
      181 tttgcctggt gctgatttaa gaacatatgt tgattttaaa aacagtacgg ttcatgttga
      241 ttcagcgggc ctggaaaaag tagcgaaatg tattaactgt aataatacgt tgcaagtaaa
      301 tatacctcat gaagccggac atgtacttgg ttacttagat gatgattatg attcttcgtc
      361 tccgtatgtt ggagatatta gtgggttgat gaatgtggga atggagcttt gggaacgata
      421 ccttaaaaac gcaactataa cgttgaatat cataatgcct gaaactaaat ttactttatt
      481 gaatgtaacc aaatgaaatt tttctgtatt atagtgattt tcctttcaat aaattacata
      541 gctatagtga atgttttcaa gtcttctgca gttgctcaat ccaccaaata ttatgaagaa
      601 aatgatatac atgatggaag taatgacatc ttttttatga agaaatatgg gccgcctgct
      661 catgagttcg tgaggccacg atataaattt gttggtgagt attatatcgg cttgaatcaa
      721 acttatcgca taacagatcc tgaatttcaa gatgttccta ttaaggaaat gttctggaat
      781 ttgcaggggg atttgaatct gacatgttgg tttcattata aagacgggca atggagagtg
      841 attacttata ttttttggcc accaggggct agattttaat ccggtattaa aggagcaaat
      901 atcatgagtt ttgtatctac gggtaataaa ccagttggaa acggtggacc cgtcatcacc
      961 acaccaccaa tagctggcga aagtggcggt atgagcacag gttcagctgt aaccgatgta
     1021 tctggtgccg ccgaggagat ggcagaacag gctgcggcgg acctgtttgg tgcgctgccg
     1081 gaaccgtcag gtctggttaa agccgcagta gctgctgcgc aggctgccgc tgcagcagca
     1141 gggatctcgg atatggctgg tgcagttcag gatgcagcgg cgtcgcttgc agccggtgct
     1201 ccgggggcgc ataacgtcac agtaagtggt agtgcggtgc cgccgcagat gcttttgttt
     1261 gcaggcatga atggttcgga agggcttggg aatctgtttt cttacacagt acagcttaaa
     1321 acgctggacg ccctgaacct ggggtacgtt tcccccgctg ctaacctgcc gcttcagctc
     1381 atggtgggca aagacctgtg cgtcagtatc gaactggacg gcggcggaaa acggtatatc
     1441 agcgggctgg tgacggcggc gagggtggtc ggccatgaga gccgttcggt aacgtatgaa
     1501 ttacgcattg agccatggct gaagctgctg acccatacca gcgactacaa agcgttccag
     1561 aacaaaaccg tggtggatat tctcgatgaa gtactggatg aatattcctt tccggtggaa
     1621 aagcggctgg tggaaaatta tccaccccgc gcctggcagg tgcagtatgg tgaaaccgac
     1681 ttcgatttca tccagcgcct gatgcaggaa tgggggattt actggtggtt tgagcacagc
     1741 gagaacagcc acacgctggt gctggtggat gccatcaatg gtcataaagc gtgccctgac
     1801 tcgccgctgg tggagtggca ccaggaaggg ctgaaactgg ataaggaatt tatccacacc
     1861 attacggcca gtgagaggct gcgtactgga aagtgggtga tggacgattt tgattttatg
     1921 aagcctcgtt cgctgcttaa aagtaccgtg gccagtccgc gtgataccgg tcatgctgaa
     1981 tacgagcatt acgaatggcc tggtgattac ttcactacgg gcgaaggcga gatgctgacg
     2041 cgcattcgta tggaagcgca gcgcagccca ggcagccggg cacatggggc cggtcatatc
     2101 cgtaccctca tgaccgggta cacctttacg ttgatgaatc acccgacggc agaaattaat
     2161 caggaatatc tgctggtaca gaccactctg tttttacggg ataatgcgca gcacagcggt
     2221 caggatcagc attttaccta tgtcaccacg tttgaactgc accccaccag cgaagtttac
     2281 cgcccacaac gaaccctgag caaaccccac accaaaggcc cgcagagcgc catcgtcacc
     2341 ggcccggcgg ggcaggaaat ctggacggat aaatatggcc gggtgaaggt gcagtttggc
     2401 tgggaccgtt acggaaagaa tgacgaaaac agttcctgct ggatccgcgt cagctatccg
     2461 tgggcgggta aaggattcgg catgatccag atcccgcgta ttggccagga agtactggtg
     2521 gatttcaaaa acggcgatcc ggatttgccc atcatcgtgg ggcgcaccta taaccaggac
     2581 accatgccgc cgtgggggct gccgggcatg gcgtcgcaga gtgggatatt cagccactca
     2641 ttgcagggag ggccgactaa cggaaatatg ctgcgcttcg atgataaaac gggtgcggaa
     2701 gaggtgaagt tccatgcgga aaaagacctc aataccacgg taaaaaacaa tgaaacccac
     2761 acggtcatgg tcgatcgcat aaaaaccatt gtcaaaaatg aaaccatcag cgtgggagaa
     2821 gaccgtaatg ccaccatcac aaaaaatgat ggtctttccg tcaaactggc tcaaaccatc
     2881 aatgtcggaa cgacatatcg tctggatgtt ggcgatcaat ttacccttcg ttgcggcaat
     2941 gcagcgcttg tattacacaa ggatggctcg attgagtttt gcggaaaaca actgttactg
     3001 cacaccagcg atgttatgca actcattggc agtggcattg atatgaatcc ggatggcggc
     3061 acagccatga ctgcggatga tattgccccg ttacctaccc ccgacgtttc tgaataaaaa
     3121 atataaaagg aatttttcta tgaccagcct ttaccgtatt caggagggct gctttgccct
     3181 gcctgaaact tttcttgatc gcaccgtcaa tatcttcgtc ccatcgggca atgaaagggc
     3241 aactcccagt ctgaatattt tccgcgatac gctcaggcca gatgaaaatc tgaccaccta
     3301 tattgaccgc cagatagcct tgatgaaaaa aaaaacctag gccagcaccg ggtactttcg
     3361 cggggaacgg cactggcggg aactggcgaa gctgccatga caggtgaaca gattctcgca
     3421 actcatcgtt ctggaaaaac agaagtgtac cagcgtcagg ccgggtttat caccgggcca
     3481 gcaaaggtac taatgttgac gctgaccact cagcgtcctt ttgatgatca caccgaccag
     3541 ctatggactg catggttgac tagttttcag cctgctaaaa gctaaaaaaa acagggaggt
     3601 tttgtcatgt atgaagccgc acgggtggat gaccccatct accacaccag cgcccttgcc
     3661 gggtttttga taggggccat cataggtatc gccattattg ccgtcgccag ctttgctttt
     3721 tttacctgcg gctttttggc cgggctgatt ttgagttttc tggctgatca gatagcttcc
     3781 ggagtcctgc aactgggtga ggctattggg cgctcgatac attccacagt gggaaaaatt
     3841 ctgaccggtt ctgataatgt cagcaccaac agtcgcccgg ccgcccgggc cgtactgagt
     3901 acggtgatat gcgaagacca ttccccggaa atccgcattg cccaaggttc cggcaacatc
     3961 tacatcaaca gccagctgcc cgtaaggatg accacactga gtgtgatgca gtgattgagg
     4021 acggctcccc caatgtcttt ctcggcggcg gtacgcagac ggtactggaa atcagcccgg
     4081 aaatccctga ctggctgcgt caggtggtgg atgtgctgta tgtggtcgcc ggaatgcttg
     4141 gcgggctggc tggcgcctgg cgtcaggcgg caaagatggg gtcgaaattt ggtacaaaat
     4201 gtgcggcaaa atttattggt ggcgaaatgg tcgggatggc catctctgac acagtcatgg
     4261 gattgttcag caatccggta gatgtcacca ctgggcaaaa aattttgctg ccggagacgg
     4321 attttaccct gccaggacgc ctgcctgtca cctgttcgcg cttttacgcc agccacctgg
     4381 aaactgaagg attgctgggc agaggatggc ggctgaactg ggagataaac ctgcgtgagg
     4441 atgaaacata tatcaccttc atcggtgtac agggtcggga actgagttat ccgaaagaga
     4501 tgctgatacc gggccaccag atattcgacc cggaagagca gttttacctg agccgtctgc
     4561 atgacggatg ctatgtgctg cattacaccg actgcagcta ttacgtgttt gatgaatttg
     4621 atgaccacgg cgtcgcgccg ttacttttta tggagacgcc gtaccggcag cgtattgctt
     4681 tcgggcgtga gaacggcagg ctggtgcggg tggcctccag cagcggacac catctgttat
     4741 tgcaccgcac gatgacgcaa gcaggggaac gcctgtcgca tattgaactt ttgaagggcg
     4801 ggcgtcccgg taacctggtg gaatatcgtt acgacgataa cgggcaactg actggcgtgg
     4861 tgaaccgggc tggtgtgacg gtgcgccagt ttgcctatga aaatggcctg atgacagagc
     4921 atcgcaacgc gacgggcttt acctgtacct accactggga agagattgaa ggtttcccgc
     4981 gcgtggtgga acacacgacc agcgacggcg aggattaccg gttccactat gattttgctg
     5041 gcgggcagac ggtggtaacc ggcaggccgg agcagaagtg gcagtggtgg tttgatgaag
     5101 agacgtatgt gactgcgcac cggacgccgg gcggcggaat gtaccgcttc acgtacaacg
     5161 aaaaccattt tccggtagcg gtggaactcc ccggcgaacg cagggtaacg ctggaatacg
     5221 acaccctgag ccgggtggtg aaagagacgg acccggcagg ccgggtgaca cagacgcagt
     5281 ggaacggcag ttttgctgaa atcacgcgcc gggcgctgga tgacgaccac gtatggaagg
     5341 cggattacaa cgaacacggc caggtgatac gggagacgga cccggaaggg cgcgtcacgc
     5401 ggtacgggta tgatgatcag gggctgccgg agacggtctg ccaccccggt aaacagcagg
     5461 accgttacac ctggaacgcg ctggggttac tgagcagcca ccgtcgcatc acgggcagtg
     5521 tgcagtcctg gcagtatacg cagcgcggga tgctggcgcg ccatacggat gaagagaaac
     5581 gggagacgcg ctggcagtac accccggaag ggctggttgc cagtctgagc aacggcaacg
     5641 gggctcagta ccggttcagc tacgacggcg acggacggct gacgggagaa cagcgcccgg
     5701 acggcctgat aaggatgttt gcgctgaatg cggacgggtt tccggtcatc atcccgacgc
     5761 agggcacaga gggcggggtg cgcaatgaac agcaggagcg tgatgcgctg ggccgcctgc
     5821 tgcgcagtga cacgcagcac agtacacgga ccttcagcta caaccgtctt gaccagataa
     5881 cggaagtgac gctcaccccc acggaagaag gggaacggct gcaccacatg caggcggaca
     5941 cggtgcgttt tgcgtatgac cgcagcgggt ggctgacggc agagcacagc gtgcacggca
     6001 gtatcaaata ccggcgcgat gcgctgggca atcccaccga tatcacgttg ccggacgggc
     6061 agcatctcag tcatctttac tacggcagcg ggcatctgct ccagacggca ctggacggta
     6121 tcacggtcag tgaatatgag cgtgacagcc tgcaccgtca ggtgatacgc acgcagggga
     6181 aactggcgac gttcagcggc tacaacgccg acaaccggtt aagctggcag cgcagcctgc
     6241 caggtggcag ccagaatcca cagcaggcgg tactcccccg gcgcagaacg acagcgtgac
     6301 ggcgcgggac tatatctgga acgcagacgg tgaagtcggc ggcatcaatg acaaactgcg
     6361 cggttgcctg gtgttcagct atgaccgcag cggctggctg accagccgca cggggcagat
     6421 gtatgaccat gaccattatt attacgacaa agcgggcaac ctgctgacgg atgagtatca
     6481 gggagcggtg atggataacc gcctgccggg ttacgggcgt gaccgctacc gctataacga
     6541 atggggcgaa ctgacagagc gcagggacca gcagctggag tggaacgcgc agggccagtt
     6601 aacgcgggtc atcagcagca acagcgagac gcggtatcag tacgatgcgc tgggcaggcg
     6661 catcagcaag gcgaccagca atctccatac cgaccgggga gaacgaagcc ggacaacgtt
     6721 tgtgtgggaa gggttccggc tgttacagga gacaacgtgg cagggaaagc ggacatacct
     6781 gtacgatgcg gaacagccgt acacgccggt ggctgccatc acggggcgcg gagagagcca
     6841 gaaaatctgg tattaccaca cggacctgac gggcacggta cacgaggtaa ccgcgccgga
     6901 cgggacgctg gtgtgggcgg gctaccaggc gggatttggt gaaaaccggg gcgacatcag
     6961 caacagcggg gcgtactttg agcagccgca gcggctgccg gggcagtact ttgacgaaga
     7021 aacagggctg cattacaatc tgttcaggta ttatgcgccg gagtgcggga ggttcgtaag
     7081 ccaggacccg ataggattaa acggtgggtt gaacttatat gcttatgcgc ctaatccgtt
     7141 aggttggatc gatccgctag gactgaattc tttaggaata gattcaaata atatatttag
     7201 aggagatggt aactataaag gtggcggaat tggacagcca aagagtggca caatatcagc
     7261 acaggacatt attgatcatc tctaagaaaa aggtgatggg cgtttaacat cttttagtac
     7321 taaaaaaaca tctagttcca actctcgtgg tgcagatttt ttctcaaaaa aacatgtctt
     7381 caaagtggca gtttctgatc tcgatgattt agttaaaaat aatcagatca ctgttttaac
     7441 acaagaaaaa gtaaaagcaa tatttggttt agaatcaagc aagaaaatta gaaaagaact
     7501 tggtaatatt cttcagaata tggctaggaa taatgaggtg ttagttcatg ggcaaattct
     7561 tggaaataaa gttagccgat gtaattagtt agtggagagg aagttgtgga cgaaattaga
     7621 ataagaattg ctggtaatac ggctgcgcct tgtttccatg tgctaaaaaa taagggatat
     7681 aaagtaacaa tggaaactca tgcattatct gacgattgtt tagattgtgt atatgattgg
     7741 tatgcggaaa aaaatggaca attttttagt gcaacgagtc cagaagagtt acttggatta
     7801 gttgcaatgt gggaagttcg tggtaatgac tggagagcta atttgaatga gataaatact
     7861 tatcaaaaag ccatagaaga atccccgttt tttgataaaa acggcaatga aatagagtct
     7921 gactaaattt tattgatata gcaacgataa taaccggaaa tttcttccag atctttccgg
     7981 ctttttatta tttaactttc gttcagcaca ctaaacatcc cgtctcgcat ccccttaact
     8041 ggctgtttaa cctgataaag ttccctgcgc agttcctgta cttcgttgtt atcccaagca
     8101 ggatcaaaca tccctccgag atcttcacgg taacgcccgt gccgatctca aatcccgctt
     8161 ttctcagcca tttggcgctg attttgatca ccggcgtgtc gctgcggttg ggcagataac
     8221 ccacggtaca gcgacgctgt gttttggaaa tggtgacttc tggcgtaata ttgtgtttaa
     8281 gttaggggct ttcgtaatct agcccagccc ccggccctaa acacaacccc cactcaccac
     8341 aacctaaact catccgcatc ccgccatgcc ggaaactttt ctctatattc ccgcaatgcc
     8401 accatcgaca gctccgcatc aatacgcgtt gccttatgcg cgtcggcggt agcgataatc
     8461 tcgccttgcg gattaatcac ccggctgtca ccgcgataat ggcagccgtt gccatcgctg
     8521 ccgacgcgat tgcatcccgc cacatacgcc tgattctcaa tcgcgcgggc cgtcagcaat
     8581 gcctgccagt gcagagagcg cggagctggc cagttggcga cgtacagggc gaggtcataa
     8641 tcgttgagat tgcgcgacca cactggaaaa cgtaagtcgt agcacaccag cggcaaaata
     8701 cgccagccgc gccattccac aatcactcgc gcattgcccg ctttataatg cagatgctca
     8761 tctgccatgc ggaacagatg acgcttatca taaaaatgta ccgtgccgcc cggctcaacc
     8821 agcaaaaagc ggttaaccga accagactcc gtttgtaatg caacactgcc tgcaatcagc
     8881 gcattgcact gctgcgcctt agctgtcatc cagttcacta cgtcatcttg tgcaagcgac
     8941 gaagctgccg cttccatggc aaagccgctg gtaaacatct ccggtagaac gatcacatcg
     9001 cgcccggtaa taccttccag ttgacgatca aaatggcgca ggttggcagg accatccacc
     9061 cacaccaacg gttgctgcaa aagcgtaatc ttcaaactag gcacggtgta caactccttt
     9121 atgcgaaggg ttttataact ttaacacctt atcaggcagt tgccttagcg cagaataaat
     9181 tgataacaaa tgctgatatt ggaaatatct gatttgcaaa ttatcgtgtt atcgctaggc
     9241 tttaggaggt taataacatg ggcaggataa gctcgggagg aatgatgttt aaggcaataa
     9301 cgacagtcgc cgcactggtc atcgccacca gtgcaatggc gcaggatgat ttaaccatta
     9361 gcagccttgc aaagggcgaa accaccaaag ctgcgtttaa tcagatggtg caagggcata
     9421 agctacctgc atgggtgatg aaaggcggta cttatactcc cgcacaaacc gtaacgttgg
     9481 gagatgagac gtatcaggtg atgagcgcgt gcaaaccgca tgactgtggc tcgcaacgta
     9541 tcgctgtgat gtggtccgag aaatctaatc agatgacggg gctgttctcg gctattgatg
     9601 agaaaacgtc gcaagagaaa ctcacctggc tgaatgtgaa cgatgcgctt tcgattgatg
     9661 gtaaaacggt gctgttcgcg gcgttgaccg gcagcctgga aaaccatccg gatggcttta
     9721 attttaaata attagcggat aaagaaacgg agcctttcgg ctccgttatt caattacgcg
     9781 gcttcaactt tccgcacttt ctccggcaac tttaccggct tcgtcgccag ctcttccgga
     9841 tcaaagtcat caacgttaat actgcgcaga cggctttctt ccgctttcac cagaatagcg
     9901 gcttcatctt tatcaatcag ccccttcgtc agcgcgttgt gcgccagttc atccagacgg
     9961 gtaaatggca gatttttacc cagctcttta cagatccgct gatgaatcgg gtcggcggca
    10021 atcacatcca ccaacgcctc ttccagtaaa ccaaccgggt tatgctcgct tggcgtcagg
    10081 tactgaccgc gaccaatacg tgaacgagtg gcgttcggca cttgtaaaat cttcgccatt
    10141 ttatgatcca gcttgtcaga cggtgccaga taatgacgtc cggtcgggaa gatcaccaca
    10201 ttcagcagcc cggcaaccac gcggttcggg aagttttgca gtaaattgtc cattgcctgt
    10261 tctgcctgat acagcgcatc ctgtacgccc cagtgcacca gcggcagatc ggcttcatta
    10321 cggccttcgt cgtcataacg cttcagaacg gcagaggcga ggtaaagctg gcttaaaata
    10381 tcccccagac gtgccgagat acgctcacga cgtttcaggc tgccgcccag cagtgccatc
    10441 gagacatcag aaagcagggc gaggttggcg ctcaggcggt tcaggtgctg atagtagcgt
    10501 ttagtggcat cgccggttgg cgtgctgctg gttaaaccgc gcgtcaggcc cagccagaag
    10561 ctgcgaactt tgttgctacc gacgtgaccg atatgtttga acaacagttt atcgaacgcg
    10621 ttgacgtcat tgttcttcgc cgcttccatc tcttccagca cgtacggatg gcaacgaatc
    10681 gctccttgtc cgaagatcat catgctgcgg gtcagaatgt tagccccttc aacggtgatg
    10741 gcaatcggtg cgccctggta agcacgcgcc aggaagttgc tttgcccgag cataatgcct
    10801 ttaccgccgg taatatccat cgcatcaata atcgactgct gcccgcggtg ggtacagtga
    10861 tacttaacga tagccgacag cacggcaggt ttttcgccga gcataatgcc gtaggtaatc
    10921 agcgatgccg cagcatccat tacataagcg ttcccggcaa tacgcgccag cggctcttca
    10981 atcccttcca tcttaccgat agagattttg aactgacggc gaatgtgaga atacgcgccc
    11041 gttgccagcg ctaccgattt cacgccgccg gttgagttgg aaggcagggt gatgccgcgg
    11101 cctaccgaga ggcactccac cagcatccgc cagccttgcc cggccatttt cggcccgccg
    11161 atgatgtaat cgatcggcac gaagacatct ttaccgcgcg tcggtccgtt ctggaacggt
    11221 acgttcagcg ggaagtggcg acgaccaatt tccacgcccg gcgtggtggt tgggatcagc
    11281 gcacaggtaa tgcctaaatc ttctgcaccg ccgagtaatt tttccgggtc ggagagttta
    11341 aacgccagcc caagcacggt cgcaatcggt gccagcgtaa tgtagcgttt gttccaggtc
    11401 agacgcatcc ccagcacctg ctggccctgc cattcgccca tgcagacaat cccggtgtcc
    11461 ggaatcgcgc ccgcatcgga acccgcttcc gggctggtca gtgcaaagca ggggatctcc
    11521 tgaccacgcg ccagacgcgg cagatagtga tctttctgct cgtcagtgcc gtaatgttgc
    11581 aacagttcgc ccgggcctaa tgagtttggc acgccgacgg taatcgccag gatcccgctc
    11641 acgccagaga gtttttgcag cacacgagac tgggcataag ctgagaactc cagtccgccg
    11701 tactcttttt tgataatcat cgcgaagaag cgatgctctt taaggtacgc ccacaactcc
    11761 ggtggcagat ccgccagctc atgagtaatc tggaaatcat tcgccatccg gcaggcttct
    11821 tctaccgggc cgtcgagaaa cgcttgctct tcagcggtca ggcgcggctg cggatagtta
    11881 tgcagctttt tccagtccgg cttgccctgg aacaagtcgc cctcccacca ggtggtgccc
    11941 gcatcaatcg cttctttctc agtgcgcgac atcggcggca tcaccttacg gaaaccgcga
    12001 aataccggcg cggaaatcat cgacttacgc ataggcgcaa agttaaatgg cacgaggata
    12061 atggtcagag gcaccagtac ccacgccgac cacagaccag caacgccgag ggcggctgtc
    12121 caggcgagca aaatcagact gctgataaat aagctcacgc ggtgatagaa caacgcgccg
    12181 agcaggacaa ccgtagcgag aatactcaaa atcatcataa cgaaaagccc cttacttgta
    12241 ggaggtctga ccacttgtga tgatatggtt gtagtggatg taaaaacatt tagcaatgtg
    12301 tttacaatat aattacaaca aagctcacat tgttgctgtt tttatccgca cttcaggtca
    12361 aaaagtcctg gtcatagcac ctgcccgtac ttctcgcttt tggcggtatc cggtacactg
    12421 cattttgtct attacattta tgctgaagga tatcctcatg taccaggatc ttattcgtaa
    12481 cgaactgaac gaagcggcgg aaacgctggc taacttttta aaagatgacg ccaatattca
    12541 cgccattcag cgcgcggcgg tcctgttagc agacagcttt aaagccggtg gcaaagtgct
    12601 ttcctgcggc aacggcggtt cccattgcga cgctatgcac tttgccgaag agttgaccgg
    12661 tcgctaccgt gaaaaccgtc cgggctaccc ggcgattgct atttctgacg ttagtcatat
    12721 ttcctgcgtc ggtaatgatt tcggtttcaa tgatattttc tcccgctacg ttgaagcggt
    12781 aggtcgcgaa ggcgatgtac tgctggggat ctccacctcc ggtaactctg caaacgtgat
    12841 caaagcgatc gcagcggcgc gtgagaaggg gatgaaagtg atcaccctga ccggtaaaga
    12901 cggcggtaaa atggctggca cggcggatat cgaaattcgc gttccgcact ttggttatgc
    12961 cgaccgcatt caggagattc acattaaagt gatccatatc ctgatccagt taattgaaaa
    13021 agagatggtt aagtaagtct ggcgtaggcc ggataaggcg tttacgccgc atccggcact
    13081 tgtgcgctga tgcctgatgc gatgctgacg cgtcttatca tgcctacaaa tctgtacccg
    13141 aaccgtaggc cgaataattc gtttacgccg catccgacct gaaaattctt aaatcaatct
    13201 tcgccggggt ccatgcgctc ccgctgttgt ggaggttacc catgtgcgaa ttgctcggga
    13261 tgagcgccaa cgtccctacc gatatctgct ttagtttcac cggacttgta cagcgtggtg
    13321 gtggaaccgg gccacataaa gatggctggg gcattacctt ttacgaaggt aaaggctgtc
    13381 gcacatttaa agatccacaa cccagcttta attcccccat cgccaaactt gtccaggact
    13441 acccgataaa atcctgttcg gtggtggctc atattcgcca ggctaatcgg ggcgaggtgg
    13501 cgctggaaaa tactcaccca tttacccgcg agttatgggg gcgtaactgg acttatgccc
    13561 ataacggaca actgacgggc tacaaatcac tggaaaccgg caacttccgc ccggtcggtg
    13621 aaaccgacag cgaaaaagcc ttttgctggc tgctgcataa attaacgcag cgttacccgc
    13681 gcacgccggg caacatggcg gcagtgttta aatatatcgc ctcactggcg gatgaactgc
    13741 gggcgaaggg cgttttcaac atgctgcttt cggacgggcg ctatgtaatg gcgtattgct
    13801 cgactaattt acactggatc acccgccgcg cgccgtttgg cgtggcaacg ttgctggatc
    13861 aggatgtgga aatcgacttc agctcgcaga ccacaccgaa tgatgtggtc acggtgattg
    13921 cgacacagcc gctgacgggc aatgaaacct ggcaaaaaat tatgccaggc gaatggcgct
    13981 tattttgcct cggggagcgt gtagtttgat gccagttgtg gctgcaccac ttcgtggctt
    14041 aacggcttgc tgaccacgta acggccattg gccacagaaa cggtcggtgg cttacgggtt
    14101 tgctcaaagt agtcgtagcc cggcttcagc tgctcccaga agtccttaaa gttggaatat
    14161 ttatggcgct tcatattggc gtcggtcatg cggaatgggt aaatactcac ctgcacgctc
    14221 ggctgaccaa acaccagcgc accagtaacg aactggaaga tctcatcaat accctgattg
    14281 gtcattgcgt agcagccgat ggatacacaa tcgccgtgga tcatcaggta tttcccttca
    14341 taaccatgcg cacggtcata agcattgggg aatccaatat taatcgcttt gtagtaacgg
    14401 ctgtctggtt ttaactgatt acgctggacg ctataaaacc cttccgggct tttgaaatcg
    14461 ccctgacgct gttttggccc taagccgccg gaatatttac agattttata actgtcgagc
    14521 agttgatatt gctcgcccat tttgacgtaa agatcgagcg tacgttcttc cttgaagatc
    14581 tggatgtaga caggggatcc catcaactgc tgcttatact ctttgctcac tggcgtggtc
    14641 gagctactgc tgcccagcaa accggcaaac gaaacgcacg ggatcaacaa catcgcaaga
    14701 attaatgcga ttttacgcat actgcttatt ccttgataaa acggttacac acgccaggac
    14761 ggcaaaatgg atcccaaatc ggaatagtct ggatttggaa ggctcacatt atcaccaaaa
    14821 gagttttacg caagcctgtc ggcgctgggt ttacaaattt agcacagggg cagttttcaa
    14881 tagtccgaaa agcgcatgta tcatttattt cctaatatta tgaaatctca atagtaatat
    14941 gcaaataagc aattaataat cccgtttact cctccggtct tttaaaattc tcaccttcat
    15001 tcagccggtt tatgtctttc atgtcttcgt tcttgctcgg cagatttttg catccaggcg
    15061 tgttttcgtt atgcgttttg ctccctttac tcgccagcgc caccacaacc gggagcagat
    15121 aaaacagcat ccagaatggt ttcctgcgcc gctcaaggcg agtgacagac gctggcaggc
    15181 gctggcggaa aacaaccact ttttaagcag cgatcacctg cataacatca ccgaagtggc
    15241 gattcaccgc ctggagcagc agcttggcaa gccttacgtc tggggcggca cgcggcccga
    15301 tcaaggcttc gattgtagtg ggttagtttt ttatgcctac aacaagatcc ttgaggcgaa
    15361 actcccgcgc acggccaatg agatgtacca ctaccgtcgg gcaacgattg tggcgaataa
    15421 cgacctgcgc cggggagatt tgctattttt ccatatccac agccgcgaga ttgccgacca
    15481 tatgggggtg tatctgggcg acggtcagtt tattgagtca ctgcgcaccg gggaaaccat
    15541 tcgggtaagc cggttagccg aacctttctg gcaggaccat tttttaggcg cgcgcaggat
    15601 tttgacggaa gagatgattt tgtaggccgg ataaggcgtt tacgccgcat ccggcggttg
    15661 tgcacggatg cctgatgcga cgctgacgcg tcttatcatg cctacaaatc tgtgcgtttt
    15721 tgcgagccgc tttcccgata taacacagcg tcattgtcgg cctttccttt tcctcttacc
    15781 gttgagaaaa aggagtcgct atgtcggaat atcgtcgtta ttacatcaag gggggaacat
    15841 ggtttttcac ggtaaattta cgcaatcgtc gaagccatct tctgaccacc cagttccaga
    15901 cgctccgtaa cgccattatt aacgttaagc gagacagacc ttttgaaatt aacgcctggg
    15961 tcgttttgcc ggagcatatg cattgcatat ggacattacc cgaaagcgat gatgattttt
    16021 cctctcgctg gcgggagatt aaaaagcagt tcacccatgc ttgcggattg aaaaatatct
    16081 ggcaaccgcg tttttgggag catgccattc gcaatacaaa agattatcgc catcatgttg
    16141 attatattta tataaatcca gagaaacatg gctgggtaaa gcaggtgagt gattggccat
    16201 tctcaacgtt ccatcgcgat gtcgcgcgag ggttatatcc catcgattgg gcgggagaca
    16261 taacggattt aagtgccggg gagcgtatca ttttataact gtgcgctggt gccggatgcg
    16321 cttacgctta tccggcctac aaatcgtgct ttaacggtag gccggataag gcgttcacgc
    16381 cgcatccggc agttatgcgc agatgcctga tgcgacgctg gcgcgtctta tcatgcctaa
    16441 aaacttgtgc ctgaacggta ggccggataa ggcgtttacg ccgcatccgg caattgtgca
    16501 ccgatgccgg attcttacat caacgccccc ataatcttca actccagctc atccggcact
    16561 tcgttatacg acagcacatg cagccccggc gcaaacaacc ttgcataacg cggcagcaaa
    16621 gggcgtagct gcggcggcac cagcagcacc ggatctttcc ccgccgcttt catctgctct
    16681 ttcacctgtg gcatcgtgct ctggaactgg ttgagcatat tcgggtcaac cggcacgctg
    16741 tcgagcatca ctttgccggc ctgttgcgcc tgattcacta cgttggtcag cagattttcc
    16801 agctcattat tcagcgtata caccgtcagc tcctgcttgc gaacgaacgg atgagtaatg
    16861 ctgcgccgca gcgccaggcg cacatcggcc gccagcagaa tatgatcttt agtcaccgtg
    16921 ctactggcga ccagcacggt ggcgatggtg acgatatcgc gcagggaaac gccttcggtc
    16981 agcagcgccc ggtacacttt cagcaattgg ctgtaattga gcgccgcgct taaatcctcc
    17041 gccaggcgcg gtgccatcga cgacaaacgg ttatgcaact gcgtgatgtc atcatagtta
    17101 aacaaatcag gaatatagct gcgcacaatc ttgttcacat gggtggcaat cacactggcg
    17161 ctgtcgatca cctgataccc catattcaga gccttcgctt tctgcgcagg ctggatccag
    17221 gtgaccggta tcccgtacgc cgggtcgttc cccagcacgc cgtcaatctc gccgtaggtt
    17281 tcgctggagg gcagcgccat cagtttatcc gccggaatat ccgcttcatc agcctcaatg
    17341 ccgttgatga aaatagcgta ctggctgggc ttgaggcgga agttttcccg aatgcggatc
    17401 tccggcagca gcacgccgtt accgtcggaa atcacctgcc gaacgccgcg aatccgctgg
    17461 gtgagcgggt tgccctgcgc tttgtccacc agcgccacca gcttgtagcc gaggcttaag
    17521 ctgatgggct cgatcagcgg aatagtttcc caactgacct gttgttcgct ggtttcagtg
    17581 atggtgcggg tcagcgtttc gaggcttttc tcttccgcct ccgccgcctg cgggcgtttg
    17641 ctcatccgcc agccggtaaa gccaagcagg gcgctgaaca gcaaaaacgg cagatgcggc
    17701 atccccggca ccaccgccag cacgaacatg atcccggtgg cggtgtagag caccgacggg
    17761 cttgccagca actggtggcg cacgtcatgg gcgatatcgc cgttgtcgct gacgcgggtg
    17821 acgataatcg ccgccgcggt ggagagcagc agggaaggga tctgcgccac caggccgtcg
    17881 ccgatggtca tcagcacgta ttgctggaag gcggcgtcgg cgctcaggtt gtatttgaag
    17941 atgccgatac agacgccgcc gctcaggttg atcgccagaa tcatcatccc ggcgatggcg
    18001 tctccgcgta caaacttcga tgccccgtcc atcgcgccgt agaaatcggc ctcgctggca
    18061 acatctttac gccgggtttg cgcctgcgcc tggttgatca atccggcgtt aagatcggcg
    18121 tcaatcgcca tctgtttgcc gggcatcgcg tccagggtaa agcgggcaga aacctcggaa
    18181 atacgctcgg cctctttggt gacgacgata aagttgattg tcaacagcgt cagtaaatcc
    18241 gagcgtgaaa gcattatcgc cgcgctgcac gggcagtcga tttttaacgg tggtgggctg
    18301 tcgccgttaa ataaaatcag cccgtctcat ccgccaaaac ctgccaccgt ggcggtgccg
    18361 gaagagacgg agaaaaaagc gcgtgatgtg aatgagaaaa cggcgctgct gaagaagaaa
    18421 tccgccaccg agcttggcga gttggcaacc agtatcaaca ccattgcccg tgatgcgcat
    18481 atggaagcga atctggagat ggagattgtt ccccagggat tacgcgtgct gattaaagac
    18541 gaccagaatc gcaatatgtt cgaacgcggc agcgcgcaga ttatgccgtt ctttaaaact
    18601 ttgctggtgg agctcgcgcc agtgttcgac tcgctctata acaaaattat tattaccggg
    18661 cataccgatg cgatggccta caaaaacaat atctacaaca actggaacct ttcgggcgat
    18721 cgcgcgcttt cagctcgtcg ggtgctggaa gaggccggaa tgccggaaga taaagtgatg
    18781 caggtaagcg caatggcgga ccagatgctg ctggacgcga agaatccgca aagcgcgggc
    18841 aatcggcgta ttgagattat ggtgctgacc aaaagtgcgt ccgatacgct gtatcaatac
    18901 tttggtcagc atggggataa agtggtgcag ccgctggtgc aaaagctgga taagcagcag
    18961 gtgctttcgc agcgaatgcg ctaaatgctg aatctttacg catttctcaa aacctgaaat
    19021 cactgtatac tttaccagtg ttgagaggtg agcaatgcgt aaaatcattc atgtggatat
    19081 ggactgcttt ttcgcagcgg tggagatgcg cgacaatccc gctctgcgcg atatccctat
    19141 tgctattggc ggcagccgcg aacgtcgggg ggtgatcagc accgccaatt atcccgcgcg
    19201 taaatttggc gtacgtagcg ctatgcctac tgggatggcg ctcaaattat gcccacatct
    19261 caccttgctt ccggggcgct ttgacgccta caaagaagcc tcaaatcata tccgtgaaat
    19321 cttctcgcgc tacacctcgc gcattgaacc gttgtcactg gatgaggctt atctcgatgt
    19381 caccgatagc gtccattgcc acggttctgc gaccctcatc gcccaggaaa tccgccagac
    19441 gatcttcaac gagctgcaac tgacggcatc tgcgggcgtg gcaccagtaa agtttctcgc
    19501 caaaatcgcc tccgacatga ataaacccaa cggtcagttt gtgattacgc cggcagaagt
    19561 tccggcattt ttacaaacct taccactgga aaaaatcccc ggcgtcggca aagtctcggc
    19621 ggcaaaactg gaagcgatgg ggctgcggac ctgcggtgat gtacaaaagt gtgatctggt
    19681 gattctgctt aaacgctttg gcaaatttgg ccgcattttg tgggagcgta gtcaggggat
    19741 tgacgagcgc gacgttaaca gcgaacggtt acgaaaatcc gtcggcgtgg aacgcacgat
    19801 ggcggaagat attcatcact ggtctgaatg tgaagcgatt atcgagcggc tgtatccgga
    19861 acttgaacgc cgtctggcaa aggtaaaacc tgatttactg attgctcgcc agggggtgaa
    19921 attaaagttc gacgattttc agcagaccac ccaggagcac gtctggccgc ggctgaataa
    19981 agctgactta atcgccaccg cgcgtaaaac ctgggatgaa cgccgcggcg ggcgcggtgt
    20041 gcgtctggtg gggctgcatg tgacgttgct tgacccgcaa atggaaagac aactggtgct
    20101 gggattatga tgtatactat tatgtatatt ctggtgtgca ttattatgag ggtatcactg
    20161 tatgcatcga attctcgctg aaaaatcggt caatatcact gagttacgta aaaacccagc
    20221 taaatacttt attgatcaac cagttgcggt tctttctaat aatcgccccg caggatatct
    20281 cttaagtgcc agcgcattcg aagcgttaat ggacatgctt gctgaacaag aggagaaaaa
    20341 gcccataaag gcgcgctttc gtccaagtgc tgcaagatta gaggaaatta cacgccgcgc
    20401 taaacaatat cttaatgata tgacggatga tgatttcaat gactttaagg aataaggatg
    20461 cgggtattca aaacaaaact tattcgcctg caacttacag cagaggaact tgatgcgtta
    20521 acggcggatt ttatttccta taagcgtgac ggtgttttgc cagatatatt tggtcgcgat
    20581 gcactctacg acgactcgtt tacctggcca ttaatcaaat ttgagcgagt tgctcatatt
    20641 catctggcaa atgtgaataa tccatttccg ccacagttgc gccaattcag cagaacgaat
    20701 gacgaagcgc atttggtata ttgtcagggt gcgtttgatg agcaagcatg gttgctcatt
    20761 gccattctga agcctgaacc tcataaactg gctcgagata acaaccaaat gcataaaatt
    20821 ggaaaaatgg cagaagcgtt tcgcatgcgt ttttgaattt atattatgaa taacatacaa
    20881 ataagagatt atcagcctgg cgattttcag caactatgcg ctattttcat tagagcggtt
    20941 acgatgaccg tcagtcagca ttattcacta caacaaattg ccgcctgggc gcagattgac
    21001 gaatctcgct ggaaggagaa actcgcgaaa tcacaagtgc gggttgcagt tattaatgca
    21061 aaactggtcg gctttattac ctgcgttgaa cactatatcg atatgttatt tgttgaccct
    21121 gaatacaccc gccgtggggt tgccagcgct ttgttaaaac cttttattaa gtctgaatcc
    21181 gaacttacgg tggacgcaag cataaccgca aaaccctttt ttgaacgtta tggttttcag
    21241 acagttaagc agcagcgcgt tgaatgccgg ggagcgtggt ttattaattt ttatatgcgt
    21301 tataaaccgc aacattaaat ccagcttgca atgaaaataa cgcccgccta gtatgtgcgc
    21361 gttttcttat tccacaaact gcaaggaggt aaatcatgac aaatccttta tcaatgactc
    21421 tttgcagacc tttccaggat taattctttt tttcttgccc tggattcgtc tgccatttcc
    21481 tgatttttta attgttactc tcaataattc aagtcgcagc acatgcaact tgaagtatga
    21541 cgagtctatt tacggaatgg tatatgggca attatattcg tcccttatcc gatgcggtat
    21601 ttaccatcgc atctgatgac ctgtggatcg agagtttagc gatccaacaa ttacacacca
    21661 cggcaaattt acccaacatg cagcgcgtag tggggatgcc agatttacac cccggacgcg
    21721 gctacccgat tggcgcagcg ttcttctccg caggccgttt ttacccagca ctggtcggca
    21781 atgatatcgg ctgtggcatg gcgctatggc aaacagatat tctcgctcgc aaatacaacg
    21841 ccgataagtt tgaaaagcga ttatcagctt tggatgacgt tgctgaagaa agctggctgg
    21901 aggaaaacct gccgtcagca tttgcacagc atccgtggcg cagctcgctt ggctctatcg
    21961 gtggtggcaa tcatttcgct gaactgcaac aagttgatca aatcatcaac gctgaactgt
    22021 ttgcactggc aggtctggat gcgcagcatc tgcaactgct ggttcatagc ggctcgaggg
    22081 ggctagggca gtctatttta cagcggcata ttgcctcgtt ttcgcatcat ggtttgcctg
    22141 aaggcagtga cgacgcgctg gcgtttgcgc gttttaatcg ccacctgatc gctttgcgca
    22201 taatgcaaca ggttaaggcc accggaagtc cggttctgga tgtggcgcat aactttgtta
    22261 gcgcgtgtcg aatcggtgat caacaagggg tcttacatcg taaaggtgcc acgcctgatg
    22321 actgtggtct ggtggttatt cccggttcac gcggtgatta ctccaggctg gttcagcccg
    22381 tcaggagtga ggaaacattg cattcgctgg cgcatggggc tgggcgtaaa tgggggcgca
    22441 ccgagtgtaa agggcgtctg gcagcgaaat acacagcgac gcaactctcg cgtactgaac
    22501 ttggcagtcg ggtaatttgt cgcgataaac aactcatctt cgaagaagcg ccacaagcct
    22561 ataaatcggc tgaaagtgtg gtgcaatgtc agggcaggcc ggattaatta ttcctgtcgc
    22621 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
    22681 taactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggac
    22741 aggctaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgctgga aacagaaaca
    22801 ggccgctact ctgacacgct gcgttcggcg ctgatttctc tggatggcga caacgcatgg
    22861 gcgttaagcg aaagctggtg tggcactatt cagtggattt gcccgagccc gtatcgccca
    22921 catcacgggc gtaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggag
    22981 caatccgatg cgatccggta tgagacgctg cgttcgtcgg ggccgggcgg tcaacatgtc
    23041 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
    23101 cagtcagagc gcagccagca tgctaacaaa cggctggcgc gattgctgat tgcctggaag
    23161 ctggatcagc agcaatagga aaatagcgcg gtgctgaaat cacagcggcg aatgttccat
    23221 caccagattg aacgtggcaa cccgcgacga acgtttacag ggatggcttt tatcgaagga
    23281 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
    23341 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
    23401 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
    23461 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
    23521 tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt cacgtaccag atgcatcacc
    23581 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
    23641 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgattagt
    23701 gaacggatca ggcagtgaat ttctacgtta ttgtcagtca tggtcaccac accgacgttc
    23761 agggaggttt cgaccacacc ttttgccacg tcggaattgc ggatcacacc gttcggggtg
    23821 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
    23881 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
    23941 atctcctgat aggtattcac cagagatttc agtgcgtcga ctttatcagc tgcgacagca
    24001 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
    24061 aggcgcaggt ccagttcttc tgcatgaccc gccaggaagc gcaccagcag tttgttggca
    24121 ttacccaggc caacgtggat ttcaccgccg gagtgaccgc ctttcagacc ttttaaggtt
    24181 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
    24241 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
    24301 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
    24361 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
    24421 accgccagtg cagaggccat accaatgccg ttatccgcac ccagcgtggt gccgcgcgct
    24481 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
    24541 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccgg tttacgattt
    24601 tccatacctg cggtagcagg ttgacgaatc aggatattac ctacctgatc gcgttcgaca
    24661 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
    24721 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
    24781 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
    24841 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtattgagaa
    24901 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
    24961 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
    25021 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
    25081 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
    25141 aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggggcg ttactggcgc
    25201 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
    25261 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
    25321 atgacctggt ggataccggt ggtactgctg ttgcgattcg tgaaatgtat ccaaaagcgc
    25381 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
    25441 atatcccgca agatacctgg atcgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
    25501 caatctccgg tcgctaatct tttcaacgcc tggcattgcc gggcgttgtt ctttttaact
    25561 tcaggcaggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
    25621 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
    25681 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg ctcttgatgg taaaaccatc
    25741 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggtat tgacccacgt
    25801 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ctgaacgtac cgacgatgat
    25861 ttatacgata ccgtgattgg ctaccgtggt ggcaactgga tttatgagtg ggccacccag
    25921 gcgatggtgt ggcaacaaaa agcctgtacg gaagaagatc cgcaactcag tggtcgacac
    25981 tggctgcatg cggctacgtt gtacaacatt gccgcctatc ctcatctgaa aggagatgac
    26041 ctggccgagc aagcgcaggc tttatcaaac cgcgcctatg aagaggccgc ccagcgtctg
    26101 ccgggcacga tgcggcagat ggagtttacc gtacccggcg gtgcgcccat caccggcttt
    26161 ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg tggtctggat
    26221 gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg
    26281 atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct cacccaggac
    26341 tccagcctgt tgcatcagca cgtcttaaag gcgctgccta acgtaccgtg ggtggatcac
    26401 actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg tctggcatac
    26461 cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc cggtagttca taccctgttg
    26521 agtgatttta agtgccagca acaggtgccg gaaatgtatc ttgacgttct ggcgagtcgt
    26581 ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg agctgaatcg ctattcatta
    26641 aaagtgcaag gattgctggg gcgtcgctgc ccaacgccaa tgttatcagg ctactggaag
    26701 aacgatccgt tcagcccgga agaggactca cgcttaatca cctcatcatc tgctgacggt
    26761 aaattattag agatcccatt taacccggtg tatcggaatt ttgacaaagg tcttcaggaa
    26821 atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg ctaaattttg ccaatttggt
    26881 aaaacagttg catcacaaca ggagatagca atgacgttac cgagtggaca cccgaagagc
    26941 agattgatca aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa gtgcgaagat
    27001 aatcgattct ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc accggaagtg
    27061 cgtgaattct ggggctggtg gatggagctg gaagcgcagg aatcccgttt tacctacagc
    27121 taccagtttg gtctgttcga taaagcaggc gactggaaga gtgttccggt aaaagatact
    27181 gaagtggttg aacgactgga gcacaccctg cgtgagtttc acgagaagct gcgtgaactg
    27241 ctgacgacgc tgaatctgaa gctggaaccg gcgaatgatt ttcgtgatga accggtgaag
    27301 ttaacggcgt gagtgaaatg tgccggatgc atcacatccg gcaatattca ttaaaactga
    27361 tacgtcatcc caaccgcgac agtatcatca ttattaatat tcagcttatt atcgctatcc
    27421 agttggttga ttttataatc aacaaacgct gacatatttt tgttgaaata atacgtagca
    27481 ccgacgtcga tataattgac cagatcttca tcaccgatgc cctcaatatc tttccctttc
    27541 gataagacat aacccagcga tggacgcaga ccaaagtcaa actggtattg agcgaccgct
    27601 tcaaagttct gtgtcttatt ggcaaagccg ccagttattg gcgtcatttt gcgtgtttca
    27661 gaatagaaag ttgccagata aatattattg gcatcgtatt tcagacctgt tgcccatgct
    27721 tctgcacgct tgcctgtgcc acggctttgc aggttctgct cgttggtgcg atctgagttg
    27781 gtataggccc cactaatggc gaaatcgctg ccgccaaagt catatgtcaa tgacgtgccg
    27841 aagccatcgc cgttttgctt tttaacgtcg cggttttcgt ttttcccttg atattgcagg
    27901 gttaagttca ggccatcgat aacgccgaag aagtcggtgt tccgatacgt cgccagaccg
    27961 ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg aatcgccacc aaattccggg
    28021 aacatatcgg tccaggcttc cacgtcatac aacgcgccca ggttacgacc ataatcgaaa
    28081 gaacccaaat ctttatattt caacccggca aaagcgagac gcgttttttg ctgtgcagta
    28141 tcactctccg ctttatttcc ggcaaactc
//
LOCUS       DAJTYM010000051        28032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTYM010000051 DAJTYM010000000
VERSION     DAJTYM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1581
                     /gene="dcuS"
                     /locus_tag="OO575_002211"
     CDS             <1..1581
                     /gene="dcuS"
                     /locus_tag="OO575_002211"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6665161.1"
                     /translation="STTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVAR
                     TLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQP
                     FKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQIGVVAIGLELSRVTQQIN
                     DSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGV
                     VAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEE
                     ITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRLDGLVNYADALRERSHEF
                     MNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGFLISKINRAT
                     DLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHG
                     WLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPG
                     IFTQFFVQIPWDGERSNR"
     gene            1578..2297
                     /gene="dcuR"
                     /locus_tag="OO575_002212"
     CDS             1578..2297
                     /gene="dcuR"
                     /locus_tag="OO575_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6665162.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            2868..4208
                     /gene="dcuB"
                     /locus_tag="OO575_002213"
     CDS             2868..4208
                     /gene="dcuB"
                     /locus_tag="OO575_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6665163.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4286..5932
                     /gene="fumB"
                     /locus_tag="OO575_002214"
     CDS             4286..5932
                     /gene="fumB"
                     /locus_tag="OO575_002214"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6665164.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6055..6684
                     /locus_tag="OO575_002215"
     CDS             6055..6684
                     /locus_tag="OO575_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6665165.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(6823..8244)
                     /gene="melB"
                     /locus_tag="OO575_002216"
     CDS             complement(6823..8244)
                     /gene="melB"
                     /locus_tag="OO575_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6665166.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8347..9702)
                     /gene="melA"
                     /locus_tag="OO575_002217"
     CDS             complement(8347..9702)
                     /gene="melA"
                     /locus_tag="OO575_002217"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6665167.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            9985..10893
                     /gene="melR"
                     /locus_tag="OO575_002218"
     CDS             9985..10893
                     /gene="melR"
                     /locus_tag="OO575_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6665168.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11092..13359
                     /gene="adiA"
                     /locus_tag="OO575_002219"
     CDS             11092..13359
                     /gene="adiA"
                     /locus_tag="OO575_002219"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6665169.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            13684..14445
                     /gene="adiY"
                     /locus_tag="OO575_002220"
     CDS             13684..14445
                     /gene="adiY"
                     /locus_tag="OO575_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6665170.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14582..15919
                     /gene="adiC"
                     /locus_tag="OO575_002221"
     CDS             14582..15919
                     /gene="adiC"
                     /locus_tag="OO575_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6665171.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16023..17666
                     /gene="eptA"
                     /locus_tag="OO575_002222"
     CDS             16023..17666
                     /gene="eptA"
                     /locus_tag="OO575_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6665172.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            17663..18331
                     /gene="basR"
                     /locus_tag="OO575_002223"
     CDS             17663..18331
                     /gene="basR"
                     /locus_tag="OO575_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6665173.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18332..19432
                     /gene="pmrB"
                     /locus_tag="OO575_002224"
     CDS             18332..19432
                     /gene="pmrB"
                     /locus_tag="OO575_002224"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6665174.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19408..19497)
                     /gene="pmrR"
                     /locus_tag="OO575_002225"
     CDS             complement(19408..19497)
                     /gene="pmrR"
                     /locus_tag="OO575_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6665175.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19609..21111)
                     /gene="proP"
                     /locus_tag="OO575_002226"
     CDS             complement(19609..21111)
                     /gene="proP"
                     /locus_tag="OO575_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6665176.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21375..22253)
                     /locus_tag="OO575_002227"
     CDS             complement(21375..22253)
                     /locus_tag="OO575_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6665177.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22250..24478)
                     /gene="crfC"
                     /locus_tag="OO575_002228"
     CDS             complement(22250..24478)
                     /gene="crfC"
                     /locus_tag="OO575_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6665178.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            24881..25216
                     /locus_tag="OO575_002229"
     CDS             24881..25216
                     /locus_tag="OO575_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6665179.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25374..25817
                     /gene="yjdN"
                     /locus_tag="OO575_002230"
     CDS             25374..25817
                     /gene="yjdN"
                     /locus_tag="OO575_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6665180.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            25950..26738
                     /gene="phnC"
                     /locus_tag="OO575_002231"
     CDS             25950..26738
                     /gene="phnC"
                     /locus_tag="OO575_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6665181.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            26763..27779
                     /gene="phnD"
                     /locus_tag="OO575_002232"
     CDS             26763..27779
                     /gene="phnD"
                     /locus_tag="OO575_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6665182.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            27885..>28032
                     /locus_tag="OO575_002233"
     CDS             27885..>28032
                     /locus_tag="OO575_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR6665183.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7195 a         7317 c         6870 g         6650 t
ORIGIN      
        1 agtaccactg tgatcttaat ggtcagtgcg gtactgttct cggtgctatt ggtggtgcat
       61 ctgatttact tctcgcaaat cagtgatatg acgcgagacg ggctggccaa caaggcactg
      121 gcagtggcgc gtaccctcgc cgactcgccg gaaatccgtc agggcttgca gaaaaaaccg
      181 caggagagtg gcatccaggc catcgcggaa gccgtgcgca aacgcaacga tctgctgttt
      241 attgtcgtta ccgatatgca aagtcttcgc tactcacatc ctgaagccca gcgtattggt
      301 cagccattta aaggtgatga catccttaat gcgctgaatg gcgaagaaaa tgtcgctatc
      361 aatcgcggtt ttctggcgca ggctttacgc gtatttaccc ccatctacga tgaaaatcat
      421 aaacaaattg gcgtggtggc gatcggcctt gagttaagcc gcgtgaccca acagatcaat
      481 gacagtcgct ggagcattat ctggtcggta ttatttggca tgctggtcgg actgattggc
      541 acctgcattc tggttaaggt actgaaaaaa atccttttcg gcctggaacc ctacgaaatc
      601 tccacgctgt ttgagcaacg ccaggccatg ttgcagtcta tcaaagaagg cgtcgttgcc
      661 gtggacgatc gcggcgaggt cacgctgatc aacgatgccg cacaagaatt gctgaattac
      721 cgtaagtcgc aggacgatga gaaactgtcg acgctcagcc actcatggtc acaggtggta
      781 gatgtctcgg aagtgttacg cgacggtacg ccgcgccgcg acgaagagat tacgattaaa
      841 gaccggctat tactgatcaa caccgttccg gtgcgcagta atggcgttat catcggtgcc
      901 atttcaacct tcagggacaa aactgaagtt cgtaaactga tgcagcgact cgacggtctg
      961 gtcaactatg ctgacgcact tcgtgaacga tcccacgaat ttatgaataa attgcatgtg
     1021 attctcggat tattgcatct gaagagttat aagcagttgg aagattacat tctcaaaaca
     1081 gccaataact atcaggaaga gattggctct ctgctgggta agatcaaatc tccggttatc
     1141 gctggttttt taatcagcaa gattaaccgc gcgaccgatt taggccatac gctgatttta
     1201 aacagtgaaa gccagctgcc agacagcggc agtgaggacc aggtcgcgac gctgattacc
     1261 acattgggaa atctgataga aaacgcgctg gaagcattag ggccggaacc cggtggcgaa
     1321 attagcgtaa cattgcacta ccgtcacggc tggctgcact gcgaagttaa cgatgatgga
     1381 ccggggatcg cacccgacaa aatcgatcac atttttgaca aaggtgtctc gacaaaagga
     1441 agcgagcgag gcgtcggttt agcacttgtc aaacaacagg tagaaaatct cggcggcagc
     1501 atcgccgtgg aatcggaacc ggggattttc acacaatttt ttgtccagat accctgggac
     1561 ggggagaggt cgaacagatg atcaatgtat taattatcga tgacgacgca atggtcgcgg
     1621 agctgaatcg ccgatacgta gcacaaatcc caggctttca atgctgtgga acagcctcga
     1681 cgctggagaa agccaaagag attatcttca atagcgatgc gcctatcgac ctgatattgc
     1741 tcgatatcta tatgcaaaaa gagaacgggc tcgatttact gcctgtcctg cataacgcgc
     1801 gttgcaaaag tgatgtgatt gtcatctcct ccgcagccga tgcggcaacc attaaagatt
     1861 cgctgcatta cggtgtcgtg gattacctga tcaaaccctt ccaggcttcc cgctttgaag
     1921 aggcgctcac cggctggcgg caaaagaaaa tggcgctgga aaaacatcag tattatgacc
     1981 aggccgagct tgatcagcta atccacggca gcagctccaa cgaacaggat cctcgtcgct
     2041 tgccaaaagg cttaacgccg cagacgttac gcacgctgtg ccagtggatt gacgcgcatc
     2101 aggactatga gttttcaacc gacgagctgg ctaacgaggt taacatctcg cgtgtttcct
     2161 gccgaaaata cctcatctgg ctggtcaact gccacatctt gttcaccagt atccattatg
     2221 gcgtcacggg gcgtccggtt taccgttacc gtattcaggc agaacactac tcactgctga
     2281 aacaatattg ccaataacgg atcgctggtt atctgtaagt aataattatt accgtaatgc
     2341 aaaatacccc tggcacaagt tacatcgtta atcagtaaaa cttgtgccag atcaaatata
     2401 attatccctc catcatcgtt aaaaattaat atctcttcag atgaacggtg tttttaattt
     2461 caaaacgata acaaaagtta attaactatt atgtcacccg cattatgtgt atttttaccc
     2521 acaaatgggt agatcagatt aatctataaa cctaatgata tctgccctga gaacaaaaaa
     2581 tagaccgata aatatcaata agataacagc aaacaaaaca ttaacatctg cgcagtacaa
     2641 actataaacc catcgccaga gagtttttct ctctgaaaaa gccgcttatc acagtgcata
     2701 aatttgccgc tgctttaatc agccaatatt cactgtgagg tatttgctaa agccggtaac
     2761 gaccaaacgg atatttagtc aggctctgaa aacagttcat acaaaacaga acgtgactgt
     2821 gatctattca gcaaaaattt aaataggatt atcgcgaggg ttcacacatg ttatttacta
     2881 tccaacttat cataatacta atatgtctgt tttatggtgc cagaaagggt ggtatcgcgc
     2941 tgggtttatt aggcggtatc ggtctggtca ttctggtctt cgtcttccac cttcagccag
     3001 gtaaaccacc agttgatgtc atgctggtta tcattgcggt ggtggcggca tcggcgacct
     3061 tgcaagcttc gggcggtctt gatgtcatgc tgcaaattgc cgagaagctg ctgcgccgca
     3121 acccgaaata tgtctcaatt gtcgcgccgt ttgtgacctg tacactgacc attctttgcg
     3181 gtacgggtca tgtggtttac accattctgc cgatcatcta cgacgtcgcc attaagaaca
     3241 acatccgtcc ggaacgtccg atggcggcaa gttctatcgg tgcacagatg gggattatcg
     3301 ccagtccggt gtcggttgcg gtcgtgtctc tggttgcgat gctgggtaat gtcacctttg
     3361 atggtcgcca tcttgagttc ctcgatctgc tggcaatcac cattccatcg acgttaatcg
     3421 gtatcctggc gatcggtatc ttcagctggt tccgcggtaa agatctggat aaagacgaag
     3481 agttccagaa attcatctcc gtaccggaaa accgtgagta tgtttacggt gataccgcga
     3541 cgctgctgga taaaaaactg ccgaaaagca actggctggc aatgtggatt ttcctcgggg
     3601 caatcgctgt agtcgccctt cttggtgctg attcggacct gcgtccatcc ttcggcggca
     3661 aaccgctgtc gatggtactg gttattcaga tgtttatgct gctgaccggg gcgctgatta
     3721 ttatcctgac caaaaccaat cccgcgtcta tctcaaaaaa cgaagtcttc cgttccggta
     3781 tgatcgccat cgtggcggtg tacggtatcg catggatggc agaaaccatg ttcggtgcgc
     3841 atatgtctga aattcagggc gtactgggtg aaatggtgaa agagtatccg tgggcgtatg
     3901 ccattgttct gctgctggtt tccaagtttg taaactctca ggctgcggcg ctggcggcga
     3961 ttgttccggt cgcgctggcg atcggcgttg atccggcata catcgtggct tcagcaccgg
     4021 cttgctacgg ttattacatc ctgccgactt atccgagcga tctggcagcg attcagtttg
     4081 accgttccgg caccacccac atcggtcgct tcgtcatcaa ccacagtttt attctgccgg
     4141 ggttgattgg tgtgagcgta tcgtgcgtct tcggctggat cttcgccgcg atgtacgggt
     4201 tcttataaat gcactttgcg tgccgcccgt gactacgcgg cacgccattt tcgaataaca
     4261 aatacagagt tacaggctgg aagctatgtc aaacaaaccc tttatctacc aggcaccttt
     4321 cccgatgggg aaagacaata ccgaatacta tctactcact tccgattacg ttagcgttgc
     4381 cgacttcgac ggcgaaacca tcctgaaagt ggaaccagaa gccctgaccc tgctggcgca
     4441 gcaagccttt cacgacgctt ctttcatgct acgtccggca caccagaaac aggttgcggc
     4501 tattcttcac gatccagaag ccagcgaaaa cgacaagtac gtggcactgc aattcttaag
     4561 aaactccgaa atcgccgcca aaggcgtgct gccgacctgc caggataccg gcaccgcgat
     4621 catcgtcggt aaaaaaggcc agcgcgtgtg gaccggtggc ggtgatgaag aagcgctgtc
     4681 gaaaggcgtc tataacacct atatcgaaga taacctgcgc tactcgcaaa acgcggcgct
     4741 ggacatgtac aaagaggtca acaccggcac taacctgcct gcgcaaatcg acctgtacgc
     4801 ggtagatggc gatgagtaca aattcctttg cgttgcgaaa ggcggcggct ctgccaacaa
     4861 aacgtatctc taccaggaaa ccaaagccct gctgactccc ggcaaactga aaaacttcct
     4921 cgtcgagaaa atgcgtaccc tcggtactgc agcctgcccg ccgtaccata tcgcgtttgt
     4981 gattggcggt acgtctgcgg aaaacaacct gaaaaccgtc aaattagcaa gcgctcacta
     5041 ttacgatgaa ctgccgacgg aagggaacga acatggtcag gcgttccgcg atgtccagct
     5101 ggaacaggaa ctgctggaag aggcccagaa actcggtctt ggcgcgcagt ttggcggtaa
     5161 atacttcgcg cacgacattc gcgttatccg tctgccacgt cacggcgcat cctgcccggt
     5221 aggcatgggc gtctcctgct ccgctgaccg taacattaaa gcgaaaatca accgcgaagg
     5281 tatctggatc gaaaaactgg aacacaatct aggtcagtac attccacaag aactgcgtca
     5341 ggccggtgaa ggcgaagcgg tgaaggtcga ccttaaccgc ccgatgaaag agatcctcgc
     5401 ccagctttcg caatatccgg tttccactcg tttgtcgctc actggcacca ttattgtggg
     5461 tcgagatatt gcacacgcca agctgaaaga gctgattgac gccggtaaag aacttccgca
     5521 gtacatcaaa gatcacccga tctactacgc gggtccggcg aaaacccctg ccggttatcc
     5581 atcaggttca cttggcccaa ccaccgcagg tcgtatggac tcctatgtgg atctgctgca
     5641 atcccacggc ggcagcatga tcatgctggc gaaaggcaac cgcagccagc aggttaccga
     5701 cgcgtgtcat aaacacggcg gcttctacct cggtagcatc ggcggtccgg cggcggtact
     5761 ggcgcagcag agcatcaagc atctggaatg cgtcgcttat ccggagctgg gtatggaagc
     5821 tatctggaaa atcgaagtag aagatttccc ggcgtttatc ctggtcgatg acaaaggtaa
     5881 cgacttcttc cagcaaatcg tcaacaaaca gtgcgcgaac tgcactaagt aacctcttcg
     5941 gcccagcgcc tggcagcatg ctgccaggtg atccccctgg gccacctctt ttgcgattgt
     6001 aatttcatgc ttgctggtga atagtcagta ttttccctga cttgcgaact caccatgacc
     6061 cgcacactca agccgttaat tcttaacact ggcgcactgg cgctaacgtt aatcctgatt
     6121 tataccggca tttcggccca tgacaaactc acctggctgc tggaagtgac accggtgatt
     6181 atcgtcgtgc cgctactact tgccaccgcc agacgttacc cgttaacgct gctgctctat
     6241 acgctcatct tctttcacgc catcatcctg atggtaggcg ggcagtacac ctacgcgaaa
     6301 gtccccgttg gttttgaggt gcaggaatgg ctggggttga gtcgtaatcc gtatgacaag
     6361 ctggggcact ttttccaggg gctggtgcct gcactggtgg cacgagaaat tctcgttcgc
     6421 gggatgtacg tgcgcggacg taaaatggtg gcgtttctgg tctgttgcgt ggcgctggcg
     6481 ataagcgcga tgtatgaatt aatcgagtgg tgggcggcac tggcgatggg ccagggagcg
     6541 gatgattttc tcggtaccca gggcgaccag tgggacacgc aatccgatat gttctgcgcg
     6601 ctgcttggcg cattaacgac agtgatattg ctcgctcgct ttcattgccg ccagctacgg
     6661 cgctatggct tgataactgg ataacaacgt gcactttgtt gatgccggat gcggcgtgaa
     6721 ttccttatcc ggtctacaaa atttgcaaat ccaaagcatt acattaagac gtggcctgat
     6781 aaacatatcg catcaggcca ttttcgttag ctattgtcca cgtcaggctt tcacatcact
     6841 cactgcaccg accggaatat cagcatgaac aggctcgggc ggtactttgc gatatttatc
     6901 cagcagatga atctggatcc tgcgcagcgt gtcgccattg aggcgataga aacggaagta
     6961 gagaatcagc gttaccatga aaaacagagt cggtagagca atcataataa actgcatacc
     7021 taatagggct tgcgtagact gttcaacatt cggtacatag ccaatcatcc ctaacacaac
     7081 cgcaatgaaa aaagccgcaa aggctgaacc gcccttcacc accatagtct gcacggagta
     7141 agcgatgctt tcacagcgta cgtgcagttt atattcaccg taatcaacgg tatctgccac
     7201 catgatgacc tgtaataccc agaaaagcgc cgttcccaca ttcagcaaaa tacccgcaat
     7261 cacaatgagg acgacgttgt gatagctcat taatgccatt aacaggagaa caccacagct
     7321 taacaccgga agaatagatg ctccggccca taaaatgcgt cgggataatg atttaactaa
     7381 gcgtgggaag aatactaacg tcaccaggtt agcagctccc gcatacgaca gataataggg
     7441 gaacaaatcc gcatcaccga taacatatga gaaataatag atagcaaatc cggtaataat
     7501 gttgctggct acattataag caagagccat acccaagagg catgaaagct gatcgttttt
     7561 ataaataagc gcaacgatgg ctttaagtgt cagatggctt ccttcagcag acgattgatt
     7621 gtctgacgaa aagacttcat gcacattgcg cagagtgatg atggttgaaa caataaaaaa
     7681 ggcgatcaat accagagtga acatctgaaa gccaaatccc cgatcgccac cgccgacata
     7741 attaacaaat ggtagcgtca cacctgccgt aacaaagcct gccagactgg caaaaaaacg
     7801 cggataagga accagttgtt cgcgttcacg tttatcgagc gtgatggttg gaaccagcga
     7861 ccagaaggga atatccataa tggtgtaagt catgccccag aggatgtagg tcacgcaaac
     7921 aaagacaatc tgagtagtac cttcaaacag atgcgcacta aagaggagaa ataagattac
     7981 agagtttgcc aacgtaccga tcaggatcca gggtttgaac ttaccccatc gcgatcgcgt
     8041 agcatttaca atccatccca taatcggatc gttaatagca tcccagatcc tcgccaccag
     8101 aaacaaagta ccgaccaaac ccacagacag cccgacgaca tcggtgtagt aatacatgag
     8161 gtacatatac acaatgccga tcgcaaaatc cttcccgaac gctccaaatc cataactgag
     8221 ttttgtagtc attgaaatgc tcatagggta tcgggtcgct gttaccaacg cccgccttct
     8281 gttattggta tcgtcagagg tgtgcgccgg aataaatccc ccggcgcagt agcgtttagt
     8341 cgcgttttaa cggtgcaacc agcttggcag ccagtcgccg tgggcggcaa tcaggtcgtc
     8401 aacaagagca tatatttcgt caatgcccag cacggcggca gtatgcgggt ccatcatcgc
     8461 ggcgtggtaa acacggtcgc gattttccgt aagaatagct tcggtcagca gcgtctgtac
     8521 gttgatgttg gtttgcatca gggcggccag atgcgaaggt agcgtaccga ctttggtcgg
     8581 ctgaatgcca ttagcatcaa ccagacaggc tacttccacg caacatcctt gtggcaggtt
     8641 atcaatcaaa ccatcgttac ggacgttgcc gtaaatcaca ctcggctcgc cagtccagat
     8701 agcgttcatg attgtgctgg catattcccg tgacggttta atatcaatcc gggaggcgtt
     8761 tttatactcc tccagctctt tatgccagtt cgccagctgc tcgacgcagc gtttcgggta
     8821 ctcatccagc ggtactttat aacgctcaat caaatcctca cgacctagct taataaacca
     8881 cggcgtatat tcggcaaaat gttctgatga ttccgtgacg aagtagccca gctttttgaa
     8941 catttcgtag cgcacaatat tctggcagcg agtattacca tgaatatttg gcttcggtgc
     9001 ctgccctgct tcataagccg ccagcagttc cgggtagaga ttcacgtaac tgccgtcggc
     9061 ggttttgcgc tccagctcca ggtaaaacgc catatggttg atacctgcgc aacggtaacg
     9121 cagcgtagcc gggtcgatat tgaggtcacg cgccagctct tccgccgttc cctgcaccga
     9181 atggcacagc ccgacctgtt tgatatacgg atagcgggca tacatcgccc aggtattcat
     9241 cgccattggg ttaacatagt tgagcatggt ggcatcgggg cagacttccg tcatgttctc
     9301 gcaaatttgc cacagatgcg gaatggtacg tagcgcgcgc ataataccgc ccggccccaa
     9361 cgtatcggca atggtttgtt ccagaccatg ccgcttacag acctcgaaat cagtcaccgt
     9421 gcaaggttca taaccgccaa tctgaaatgc caccacgaca aaatcggcat cctctaaggc
     9481 ttctttctgt tgggtgtggc aggtgatttt gccgctggcc cctgctgaat ccatcagctt
     9541 acgcaccaca atatgcgact cttccaggcg ggtggggtca atgtccatca gggcaatatg
     9601 cgccgttttc agcgcctcgc gatggaacac atcaccaaga atatttttaa cgaaaatcgt
     9661 cgaaccagcg ccgataaatg taattttggg tgcagacatc atgcagatct cctggcttgc
     9721 ttgaataact tcatcatggc aggcgaatct gcagaaaacc tccggcttcc aggttgagca
     9781 ttcccataaa ctcagatcct gcgtgaagca gcagtaaatc tgagtttatg ggaataaatt
     9841 acctgaaaag tagagttttc ggcctcatga tgctcggaca gtcccctcgc ccctctggga
     9901 agagggtgaa aaccgtgctc ccactcgcag tcatcctccc tcactcctgc cataattctg
     9961 atattctagg aaagagagcc atccatgaat acagatacgt ttatgtgcag cagcgacgaa
    10021 aaacagaccc gcagcccgct gtcgctgtat tcggaatacc agcggatgga gattgagttt
    10081 cgcgcaccgc atatcatgcc caccagccac tggcatggtc aggtcgaagt gaatgtgcct
    10141 ttcgatggcg atgtggaata cctgatcaac aatgaaaaag tgaatatcaa tcagggtcat
    10201 atcacgctgt tctgggcctg tacaccgcac caactaacag ataccggaac ctgtcagagc
    10261 atggcgattt ttaatctgcc gatgcatctg tttctctcct ggccgctgga taaagacctg
    10321 attaaccacg tcactcacgg catggtgatc aaatcacttg ccacacagca acttagcccg
    10381 tttgaagtac gccgctggca gcaggaatta aacagtccga atgagcaaat tcgccagctc
    10441 gccattgatg aaattggcct gatgctcaag cgatttagcc tctctagctg ggaaccgatt
    10501 ctggtcaata aaacctcgcg cacacacaaa aacagcgtct cgcgccatgc gcaattttat
    10561 gttagccaga tgctgggctt tattgccgaa aactatgatc aggcgctgac catcaacgat
    10621 gtggctgagc acgtcaaact taacgccaac tatgcaatgg ggatattcca gcgggtcatg
    10681 caattgacga tgaaacagta cattaccgcg atgcgcatca accacgttcg cgcgttactg
    10741 agcgataccg ataaaagtat tctcgatatt gccctgacgg caggctttcg ttcaagtagt
    10801 cgtttttaca gcacgttcgg caaatatgtc ggcatgtcgc cgcaacaata ccgcaaactt
    10861 agccaacagc gccgccagac gtttcccggc taaagacgat atcagtatca gccaaaaaaa
    10921 tagtttagcc gtcgataata agcagtgaaa ataatttaaa ataatcacat aacgtattgt
    10981 ttataaaact aaatatttca aaaatgtttg tttttcacgc gctttacagc ccgaaaaggc
    11041 cggaagatac ttgcccgcaa cgaagattcc ttcataaccg ggtaagcaat gatgaaagta
    11101 ttaattgttg aaagcgagtt tctccatcaa gacacctggg tcggtaacgc cgttgagcgt
    11161 ctggcagatg ctttaagcca gcaaaatgtt accgtgatta aatccacctc ctttgatgat
    11221 ggttttgcca ttctctcttc aaacgaagcc attgactgcc tgatgttcag ctatcaaatg
    11281 gagcatccgg acgaacatca aaacgtcaga caattgatcg gtaagcttca tgagcgccaa
    11341 caaaacgtgc cggtcttcct gttgggcgat cgggaaaaag ccctcgccgc aatggaccgg
    11401 gatctgctgg agcttgtcga tgaattcgcc tggattctgg aagataccgc cgactttatc
    11461 gccggacgcg ccgttgccgc gatgacccgc taccgccagc agctgttgcc gccactgttc
    11521 agcgcgctga tgaaatatag tgacatccat gaatattcct gggcagcgcc aggccaccag
    11581 ggcggcgttg gttttaccaa aacacccgcc ggacgtttct accatgacta ctatggtgaa
    11641 aatctgttcc gtaccgacat gggcatcgaa cgaacttccc tcggttcttt gcttgaccat
    11701 actggcgcat ttggcgaaag cgaaaaatat gccgcacgcg tatttggtgc cgatcgctcc
    11761 tggtcggtag tcgtcggtac ttccggctct aaccgcacca tcatgcaggc ttgcatgacc
    11821 gataacgatg tcgtggtcgt tgaccgtaac tgccataaat ccatcgaaca aggtttgatg
    11881 ctgacaggcg cgaaaccggt ctatatggtg ccaagccgca accgctacgg cattatcggg
    11941 ccaatctatc cgcaggaaat gcaacctgaa accttgcaaa agaaaatcag tgaaagcccg
    12001 ctgaccaaag acaaagccgg gcaaaaaccg tcttactgcg tggtaaccaa ctgcacctat
    12061 gacggcgtgt gttataacgc taaagaagcg caggatctgc tggaaaaaac ctccgatcgt
    12121 ctgcactttg acgaagcctg gtacggctat gcacgtttca acccgatcta tgccgatcac
    12181 tatgccatgc gcggcgaacc tggcgatcac aacggtccta ccgttttcgc cacccactcc
    12241 atccacaaac tgctgaatgc gctgtcacag gcttcttata ttcatgtacg tgaaggtcgt
    12301 ggggcgatta acttctcccg cttcaaccag gcctacatga tgcatgccac cacctccccg
    12361 ctgtatgcca tctgcgcatc caacgacgtg gcggtgtcga tgatggacgg caacagcggc
    12421 ctgtcactga cacaggaagt gattgacgaa gcggttgatt tccgtcaggc gatggcgcgg
    12481 ctatataaag agttcaccgc tgacggtagc tggttcttca aaccgtggaa caaagaagtc
    12541 gtcaccgacc cacaaaccgg caaaacctat gactttgctg acgcaccaac caaactgctg
    12601 accaccgttc aggactgctg ggtaatgcat ccgggcgaaa gctggcacgg cttcaaagat
    12661 attccggata actggagtat gctcgaccca attaaagtca gcatccttgc tccgggaatg
    12721 ggtgaagatg gtgaactgga agaaaccggt gttccggcgg cgctggtcac tgcctggctt
    12781 ggtcgccacg gcattgtgcc tacccgcacc actgacttcc aaattatgtt cctgttctct
    12841 atgggcgtaa cccgtgggaa atgggggact ctggttaaca ccctttgctc cttcaaacac
    12901 cactatgacg ccaacacacc gctggcgcag gtgatgccgg aacttgttga acaatatcct
    12961 gacacttacg cgaacatggg gattcacgat ctgggtgaca ccatgtttgc ctggctgaaa
    13021 gaaaacaacc ctggcgcacg gttgaacgaa gcctattccg gcctgccggt ggcggaaatc
    13081 accccgcgtg aagcgtacaa cgcgattgtc gacaacaatg tcgaactggt atccattgaa
    13141 aatctgccag gacgcatcgc ggcaaactca gttatcccgt atccgccagg aatcccgatg
    13201 ctgctgtctg gtgaaaactt cggcgataaa aacagtccgc aagtaagtta tttacgctcg
    13261 ctgcaatcct gggaccacca tttccctaga tttgaacacg aaactgaagg gactgaaatt
    13321 attgacggta tttaccacgt tatgtgcgtg aaagcgtaac cactattccg ctgaaggcgt
    13381 aattgtttaa ataacattac gccgcctggc cttaggcctt ttgagtatgg caacgttttc
    13441 ataaaaattg ctgcaaacaa aaatgtcata ctttttgcgc ggccccaccc cacgcttttt
    13501 tttgcctgtt atttatcctg taaaaaaata tgtacatgag aaattactat aaaaatttgt
    13561 aatattagta aaactcgtta tttttatgca tgtttatatt catcatgcaa ttatataacc
    13621 atttcccggt atcgctttgc tttagcgaga actggtcttt tattcgcact caggagtaca
    13681 tgtatgagga tttgcagcga ccaaccttgt attgttttat tgactgaaaa agatgtctgg
    13741 ataagggtga atgggaaaga acctattagc cttaaagcta accatatggc gttattaaat
    13801 tgtgaaaata atattatcga cgtctcctct cttaacaaca ctttggttgc tcatattagt
    13861 cacgacatca tcaaagatta cctccggttt ctgaataaag atctctcgca aataccagta
    13921 tggcaacgta gcgctacgcc catactcacc ctgccatgcc tgacgccaga cgtctttcgc
    13981 gttgccgcgc aacacagcat gatgcccgca gaaactgagt cagaaaagga acgaacacgc
    14041 gcattattat tcacggtgct atcccgtttt ctcgacagta aaaaattcct ttcattaatg
    14101 atgtatatgt tacgtaattg tgtaagtgac agcgtttatc aaattattga aagcgatatt
    14161 cacaaagact ggaatcttag tatggtagcc agttgtttat gtcttagccc aagtctgtta
    14221 aagaaaaagt tgaaaagcga aaacaccagt tatagccaaa taatcaccac ctgccgcatg
    14281 cgttatgccg taaatgaatt aatgatggac ggtaaaaata tctctcaggt atcacagtcc
    14341 tgcggctaca acagtacgtc gtactttatt tctgtcttta aagacttcta cggtatgacg
    14401 ccgctgcatt atgttagtca gcacagagaa cgcactgtcg cctgattttt aaccttaacg
    14461 aagagctata ttaataacgg catcagcgat aacccggtcg ataataattc aactatcgaa
    14521 tgcaggcgta tgatatgacg taatttattg tcacgaagct cgccttcgca ggagtttaat
    14581 tatgtcttcg gatgctgatg ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc
    14641 ggggaatatt atggggtcag gtgtttttct gttacctgca aacctggcct ccactggcgg
    14701 gattgccatt tatggatggt tggtgacgat tatcggtgcg ctggggctct cgatggtata
    14761 cgccaaaatg tcgttcctcg acccaagtcc tggtggttct tacgcttacg cccgccgctg
    14821 ctttggcccg tttctcggtt atcaaaccaa cgtcctctac tggctggcct gctggatcgg
    14881 caatatcgcc atggtggtca ttggcgtagg atatttaagt tacttcttcc cgattctgaa
    14941 agatccattg gtattaacca tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa
    15001 cattgtcggt ccgaaaatga tcacccgcgt gcaggccgtt gccactgtgc tggcgctgat
    15061 ccccatcgtc gggattgccg tatttggctg gttctggttc cgtggtgaaa cctatatggc
    15121 ggcatggaac gtcagcggcc tgggcacctt cggtgcgatt caaagtaccc ttaacgttac
    15181 gctgtggtcg ttcatcggtg tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc
    15241 gaaacgcaat gtccctatcg ccaccattgg tggggtattg attgccgccg tttgctatgt
    15301 gctctccacc accgcgatta tggggatgat ccctaatgcc gcactgcgcg tttctgcctc
    15361 gccattcggt gatgccgcac ggatggcgtt gggtgacacc gctggggcca ttgtttcctt
    15421 ctgcgcagct gcgggttgct taggttcact gggcggctgg acgttgctgg cgggtcaaac
    15481 ggcgaaagcc gctgccgatg acggactgtt cccaccgatt tttgcccgtg taaataaagc
    15541 gggtacgcca gtggcggggc tgattatcgt cggtattttg atgaccatct tccagctcag
    15601 cagcatttca ccaaacgcga ccaaagagtt cggtctggtt tcttccgtct cggtcatctt
    15661 tacactggtg ccatatcttt acacctgtgc ggcgttactg ctgctcggcc acggtcactt
    15721 tggtaaagca cgcccggcat atctggctgt tactaccatt gccttcctct actgcatttg
    15781 ggccgtggtg gggtccgggg cgaaagaggt tatgtggtca tttgtcaccc tgatggtcat
    15841 caccgccatg tatgcgctga attacaaccg gctacataaa aacccgtatc ccttagatgc
    15901 accaataagc aaagattaat tccccttaat ccagcaaaca taaaagccaa ccttaagaac
    15961 ttaaggttgg cttaattttg ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg
    16021 tgatgttgaa gcgcctacta aaaagaccct ctttgaattt actcgcctgg ctattgttgg
    16081 ccgcttttta tatctctatc tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc
    16141 tgccgctgga ttcgctgcat aacgtactgg ttttcttgtc gatgccggtt gtcgctttca
    16201 gcgtgattaa tattgtcctg acactaagct ctttcttatg gcttaatcga ccactggcct
    16261 gcctgtttat tctgattggc gcggctgcac aatatttcat aatgacttac ggcatcgtca
    16321 tcgaccgctc gatgattgcc aatattattg ataccactcc agcagaaagt tatgcgctga
    16381 tgacaccgca aatgttatta acgctgggat tcagcggcgt gcttgctgag ctgattgcct
    16441 gctggctaaa aatcaaacct gccacctcgc gtctgcgcag tgttcttttc cgtggagcca
    16501 atattctggt ttctgtactg ctgattttgc tggtcgccgc actgttttat aaagactacg
    16561 cctcgttgtt ccgcaataac aaagagctgg tgaaatcctt aagcccctct aacagcattg
    16621 ttgccagctg gtcatggtac tcccatcagc gactggcaaa tctgccgctg gtgcgaattg
    16681 gtgaagacgc gcaccgcaac ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc
    16741 tgattgtcgg cgaaacctcg cgggcggaga acttctccct caacggctac ccgcgtgaaa
    16801 ctaacccgcg gctggcgaaa gataacgtgg tctatttccc taataccgca tcttgcggca
    16861 cggcaacggc agtttcagta ccgtgcatgt tctcggatat gccgcgtgag cactacaaag
    16921 aagagctggc acagcaccag gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg
    16981 tgctgtggaa tgacaacgat ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga
    17041 acgtcaccgc gctgaatctg cctgatcagt gcatcaacgg cgaatgctat gacgaagtgc
    17101 tgttccacgg gctggaagag tacatcaata acctgcaggg tgatggcgtg attgtcttac
    17161 acaccatcgg cagccacggt ccgacctatt acaaccgcta tccgccgcag ttcaggaaat
    17221 ttaccccaac ctgcgacacc aatgagatcc agacctgtac caaagagcaa ctggtgaaca
    17281 cttacgacaa cacgctggtt tacgtcgact atattgttga taaagcgatt aatctgctta
    17341 aagaacatca ggataaattt accaccagcc tggtttatct ttctgaccac ggtgaatcgt
    17401 taggtgaaaa tggcatctat ctgcacggtc tgccttatgc catcgccccg gatagccaaa
    17461 aacaggtgcc aatgctgctg tggctgtcgg aggattatca aaagcggtat caggttgacc
    17521 agaactgcct gcaaaaacag gcgcaaacgc aacactattc acaagacaat ttattctcca
    17581 cgctattggg attaactggc gttgagacga agtattacca ggccgcggat gatattctgc
    17641 aaacttgcag gagagtgagt aaatgaaaat tctgattgtt gaagacgata cgctgttatt
    17701 gcagggactg attctggcgg cgcaaaccga aggctacgcg tgcgatggcg tgacaaccgc
    17761 gcggatggcg gaacaaagcc ttgaggacgg tcattacagc ctggtggtac tggatttagg
    17821 gttacccgac gaagatggac tgcattttct cgcccgtatc cggcagaaaa aatacaccct
    17881 gccggtactg atcctcaccg ctcgcgatac gctgaccgac aaaatcgccg ggctggatgt
    17941 cggtgccgat gactatctgg tgaagccttt tgcgctggaa gagttacacg cccgtatccg
    18001 cgccctgcta cgacgccata ataatcaggg cgaaagtgag ctgattgttg gcaatctgac
    18061 gctgaatatg ggtcgccgtc aggtatggat gggcggtgaa gagttgattc tgacgcccaa
    18121 agaatatgct ctgctgtcac ggttaatgct caaagcaggc agtccggtac atcgggaaat
    18181 tctctacaac gacatctata actgggacaa tgaaccctcg accaacaccc tggaagtgca
    18241 tatccacaat ctgcgcgaca aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg
    18301 ctatatgctg gtcgcgaatg aggaaaacta attgaatctg atgcgttttc tgcgccgacc
    18361 tatatcgctg cgccaacggc tgatattgac catcggggcc attttgttgg tgtttgagct
    18421 gatcagcgtc ttctggctat ggcatgaaag taccgagcag attcagctgt ttgagcaggc
    18481 gctacgcgac aatcgcaaca acgatcgcca catcatgcgt gagatccgcg aggcggtcgc
    18541 cagcctgatt gtccccggcg tctttatggt cagcctgacg ctatttatct gctatcaggc
    18601 ggtacgccgc atcacccgcc cgctggcgga gctgcaaaaa gagctggaag cgcgcaccgc
    18661 cgacaactta acgcccattg ccattcacag cgccaccctc gaaatcgaag cggtggtttc
    18721 ggcgttgaac gatctggtca gtcgcctgac cagcacgctg gataacgaaa ggttgtttac
    18781 cgctgacgtc gcgcacgaac tgcgaacgcc actggcgggg gtgcgtttgc atctggaact
    18841 gctggcgaaa acgcatcaca ttgatgtagc accgttagtg gcacggcttg atcagatgat
    18901 ggagagcgtc tcccagctgc tgcaactggc gcgtgccgga cagtcatttt cttccggtaa
    18961 ttatcaacat gtaaaactgc tggaagatgt cattctcccc tcgtatgacg aactcagtac
    19021 catgctcgac cagcgacagc aaaccctgct attgccagag agcgccgccg acatcaccgt
    19081 tcagggtgat gcaaccctgc tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg
    19141 ttacagcccg caaggcagca acattatgat taagctgcaa gaagatggcg gagcggtcat
    19201 ggcagttgaa gatgaaggac caggtattga tgagagtaaa tgcggggagt tgagtaaagc
    19261 gtttgtacgt atggacagcc gttatggcgg gattggtctg gggttaagta ttgtcagccg
    19321 cattacacag ttgcatcacg ggcagttttt cctgcaaaac cggcaagaga cttccggcac
    19381 gcgggcctgg gtacggctga agaaagatca gaacgtggca aaccagatat aaagaaagct
    19441 gctgaccacc agcacgctga acacggtagt taaactttca taaacacggt ttttcattgc
    19501 actctcctcc gttaacctgg aggagagtat gcgcgtcaga gattaagtga accttaagag
    19561 ttcaattggc ctgataagac agcgtcacat caggccatcc gtttcagctt attcatcaat
    19621 tcgcggatgt tgctgcacca ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc
    19681 aatattatcg attttctgct cgatattatc gtaatgctcg acgagaattt ccttcgcttc
    19741 ctgtatatct gacgccgccg gtgtcgcacc tttcaacgga cgatttgccg tctctttcat
    19801 ggttacgccg gtgattaaac caatcaccgc cactaccatc aggtaatagg caggcatcat
    19861 cagattctgc gagctttcga ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag
    19921 caccgaaata ttaaatgccg ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc
    19981 tggcaaggta gaagccataa cgcccataaa gcagttaagg atcaccgcca gcatcagtaa
    20041 cccggcaaaa atcaggccga tgacgttact gttaatcaga ataaacgccg ggatcgccaa
    20101 cacaaacagt gcaacactac caagtagcac aaacggacga cggccaaaac ggtcactcag
    20161 caagcccatc accggctgga caaacagcat accgatcata atggcgataa taatcagcac
    20221 cccgtggtct tcggagtaat gcaggttatg cgacaaataa ctcggcatat aggtcagcaa
    20281 catgtagtaa gtcacgttgg tggcaattac cagaccaata catgtcaaca ggctgcgcca
    20341 gtatttagtg gcaatctctt taaacgagac tttcgggcca tcctgcaaac cttcgcggtc
    20401 gccctgttcc agtttatcaa catgctgctg gaacgccgga gtttcttcca gcgcatggcg
    20461 caggtaaagc ccgataatcc ctaacggcag agcaataaag aacggaatac gccagcccca
    20521 gtcgaggaag ttcgcttcgc cgacaatggt cgaaattaac accaccacgc ccgcacccag
    20581 cacaaacccg gcaatagaac cgaagtccag ccagctgccc ataaagccac gtttacggtc
    20641 aggggagtat tccgcaacaa atatcgacgc cccggtatat tcaccaccga ccgagaaacc
    20701 ttgtgccatc ttacagatca acagcagaat cggtgcccaa ataccaatcg tgtcgtagga
    20761 cggtattaag ccaatacaga acgtactgat cgacataatc acaatagtga tagcgaggat
    20821 cttctggcga ccatatttat cgcccaacat accaaagaag agtccgccaa gcggtcgaat
    20881 cagaaaggga acggagaaag tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc
    20941 cgggaaaaaa actttaccta atgcgtaagc aacaaaacca taaacaccaa aatcaaacca
    21001 ttccattgca ttacccagtg atgctgcggt aatggcttta cgcagtttac cgtcatcaat
    21061 aatggtgaca tcacgaaggg taatcggttt tacttttttc cttttcagca tagctttcct
    21121 cgcagagttg gcccatttcc gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg
    21181 agcgggaatt gcaggatgca atctctgtaa caagcgtagc gggtttgctt acaccctccg
    21241 gttaaggaat gtacaatcaa accagttgac gcctgaattg ggcagttaat gaccttttta
    21301 ccctaccagt gtttaaattt gtgatcaact tcacacatgg atttcgttta ggactcattg
    21361 atgtaactga ttatttacgc caaatttaca gagaagattt cctcgaccat tgcccccacc
    21421 aaacgctgcg ctgaacagag tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc
    21481 actgaccagc aaacgggttg tttatctgcc gtggtccact ttcccagcca acttaaaaaa
    21541 tcagcatgat ccccgcccgt cggaacggtc gaagaggcaa accactgatg gtaatagtga
    21601 cgcgtagtcg gtgccgcatt gatactctct gccagatgtt gtgtcgcttt ctcgcgcaaa
    21661 tcacccagca acaacaccac cgcttgcggg cttcccagac ggcaggcatc gccaaatgca
    21721 ccccagcgca actcctccag aacgataaaa cagcgcccag gcaacgacca ggcgttatac
    21781 gccccagcac gccagcgggt aaaaatttgc tcgctctgat cacctgcctg caccagtagg
    21841 cctcgttgcg ttaacgcctt ttccttccag cgacaacggg ctgaaaacca ctcatacaag
    21901 gcggcaattt gctgggggag ctccgggaaa gcagagtgct ggcggtgatc cattttttgc
    21961 agcagcgcca gatgctgcgc gatctgcgtc agcgccagat gaccgggatc cagcatcgac
    22021 gccagttttt ccaccattgt cagtcgcagg ctaagcggcg catttaacat gccgccgttc
    22081 gtccatgtct gaagctgcac acgcgaaaga agttcctgca tcagtcgttc acgaaactgc
    22141 tgctgatggg cttgaggatg gcgggcatca tcgccctgcg ccagatccac taaaaagcgc
    22201 ggatgaaccg actccagcac gcgaccgggg ccgtccagta acgtcttggt caatatcgtt
    22261 ctgccgtgaa aagtgtttga atatcatcgc gtaacagctg agcgtcggtg taaatccagc
    22321 tgtgagtcat cacagtctgc tgcaattgct gctgcatcag cctgaccacc gattcatttt
    22381 gttgacgcag agccagactt tcgcgtaaac gcgtctgtaa ttctgtcaaa catgaagcga
    22441 actcagcgaa aaaagtattc atgcctgccg taacagattc atcgacctgc tctgccagaa
    22501 ctttagccat ttgttggcaa tagagatcga cttctgcgct taatgctcgt tgcaacacac
    22561 tgtaatcaac cgtttctgtc ggggatttct catttccccg tccccagtcg ggctgattca
    22621 accagcgcga aaaagtctca cgcacaacgc ctaaacgcgt gctctgctcg tccgttgctt
    22681 cctggcgcga aatgacttca ctgaacagct ggcgagtgtt gaagtgggga actacgccgt
    22741 gaaaaacagg aaaatgaaac ccaggacgaa accctgactc gctcaattcc attttgactt
    22801 gttgctcaat ggggcgaata acatcggtta acacccggca aagggtggat tccatctcgg
    22861 caaaacgcag cgtaaagtcg cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac
    22921 agcgggtacg aatctcgctt aacggctccg aatcatcctg aaacaaggcg gctaactgcg
    22981 cattcaccgc atcttgctgt tgacgcagaa agtggttggc ggaggtcagg gccagctcga
    23041 tttcatgttt aatctcgccg ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt
    23101 cttcgacctg atggatattt tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac
    23161 gaaaatccag gtattcccgc gcctgctgcg cgtaattcaa cagtttatgc gcagcagatc
    23221 gcagagcata caatgaggcg ttagcgtaag cggcatgaag caacgcctga attggctggg
    23281 cgaacagcga atcttcccac aactgatcgg cagcatgacg aatatgttcg aggtccgcca
    23341 gatcggcatg acgccagcgc ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt
    23401 gttgctctgg cgctggtaac ttaccgttgt tggctaactc atagcgcgcc cgattcgcca
    23461 ggtagcccca catcgacgac accgggaata tctgctgtgg cgtaatacag cctttcatca
    23521 gcgtcccgga aatcagtgcc cgcacctggt cggcgtcgtc actgttacga tcctgttgat
    23581 cgaacttatt gaccagcaca tacagcggca ccgattgccc caccgccaaa atcgcctcac
    23641 ggacctcttc atcggagatc gatttcagtt gcgtataatc cagcaccgcc agtaccgccg
    23701 aggcgcgtgc cagctgctgg ttaagcattt tttgcagatg cggttgcccg gcttcatttg
    23761 gcccgggggt atccagtaac gtcaactgac cgggataact ttccagcccc gccagatgga
    23821 caaactccac ttcaatcacg ggaatatgct caatggcggc gtaagcagaa aaaggaaaat
    23881 cgacgtccag cgccttcgcc agtcgcacta aatcattcaa acttttcaga caatgaaaaa
    23941 taggctgggc acccagataa tatttttcga aagcgacgcc attttcgatc cgctgcataa
    24001 gcgcacgcat atctttatct atttccagca catcggtcag atgcttaata tcgcaatcac
    24061 gcaggcgctg ttgtaattgt tgaattaaac aatcgattgg cgcgacatgt gaaaaatgca
    24121 gtaccggttc cttttgcccg ggcgtatggc gaataagcgt cggcagcgca gtcattgggc
    24181 gattacgatt aggcagaacc tccgtaccaa caatggcatt aatggtggtt gatttccctg
    24241 ctttcatggt accgacaatt gccagcacca tttccagtcg ggaaatttta cacaactcat
    24301 tattcagcat cgcgtgacgt tcggcgatat taggctgact ccagggtaaa gccagttgtg
    24361 gcgcgtcgtc tccgggtaca gagagaggca ttttttccag taactgcaac tgttggcgag
    24421 aaagctgtaa caggcgttca gcctcctgac ttaactcata cagggtctgt gtgtacatag
    24481 aaaattcttc cttaaagcaa attttgttac ttttatttag ccagattgtt tttgagttct
    24541 gttttcggct tttataatta ctgcaagaaa taattttata tttagtgtgt tgttttttta
    24601 tcagaataaa taacgtcttc tgatgcgttt aaaacgtcag aaagataaaa atatcatgtg
    24661 aataaaaaaa agaacaagta gagcattaac attatcttaa ataataaata gaggcaaaaa
    24721 gattattttc tttttgcgtt tcctttcaaa tgaaaacgat cgtcgtctaa aatcagcagt
    24781 acccccgaca aactcaggga ttttgtgtat aattgcggcc tttttcggca atctgccgtt
    24841 ttttggcgct tttgccctgc tgacttttga ggaaatccac atgtcattac cacactgccc
    24901 aaaatgcaac tccgaataca cttacgaaga taacggcatg tacatctgcc cggaatgtgc
    24961 ctacgaatgg aacgacgcag aacctgcaca ggaaagcgac gagctgatcg ttaaagatgc
    25021 taacggcaat ctgctggctg acggcgacag cgttaccatc attaaagatc tgaaggtgaa
    25081 aggtagctct tcgatgctga aaattggcac caaagtgaaa aacatccgcc tggttgaagg
    25141 cgaccataac atcgattgca aaatcgacgg ttttggtccg atgaaactga aatctgagtt
    25201 tgtgaaaaag aactgattgt attgtgatcg gtaagccgga taaggcgctc gcgccgcatc
    25261 cggcaacgat gccgactgcc tgatgcgacg cttgcgcgtc ttatcaggca gcctctcatc
    25321 tgtataaatt tcgaactaca cttaactggc ttctcttaac tgaggtcacc atcatgccgt
    25381 taagtcccta cctctctttt gccggtaact gttccgacgc gattgcctat tatcaacgta
    25441 cgttgggcgc ggaactgctc tataaaatca gcttcggcga aatgccgaaa tcagcgcagg
    25501 acagcgccga gaactgccct tccggaatgc aatttcccga taccgccatc gctcatgcca
    25561 acgtgcgcat tgccggaagc gacatcatga tgagcgatgc tataccgtca ggaaaagcca
    25621 gctactccgg ctttacgctg gtgctcgatt cgcaacaggt cgaagaagga aaacgctggt
    25681 ttgataacct tgccgctaac ggaaaaatcg aaatggcctg gcaggaaact ttctgggcgc
    25741 atggctttgg caaagtcacc gataaatttg gcgtaccgtg gatgattaat gtcgtcaaac
    25801 aacaaccaac gcaataaccc gccgggaggc ccgccctccc gcactgtcat cgaattcccg
    25861 ttaactcttc atctgttagt cacttttaat taaccaaatc gtcacaataa tccgccacga
    25921 tggagccact tttttaggga ggctgcatca tgcaaacgat tatccgtgtc gagaagctcg
    25981 ccaaaacctt caatcagcat caagcgctgc atgcggttga tctgaacatt catcacagtg
    26041 aaatggtggc tctgcttggg ccgtcgggtt ccggcaaatc caccctttta cgtcatttaa
    26101 gcggtttgat taccggcgat aaatccgccg gtagccatat cgagctactg ggccgcactg
    26161 tccagcgcga aggccgcctg gcccgcgata tccgcaaaag ccgcgccaac accggctaca
    26221 tctttcaaca attcaacctg gtgaaccgcc tgagcgtact ggagaacgtg ctgattggcg
    26281 cgctcggcag cacgccgttc tggcgcacct gttttagctg gttcaccggc gagcagaaac
    26341 agcgcgcgtt acaggcgctg acccgcgttg gcatggtgca ttttgcccat cagcgcgttt
    26401 ccaccctctc cggcggccag cagcaacgtg tggcgattgc ccgtgcgctg atgcagcagg
    26461 cgaaagtgat tctggccgat gaacccatcg cctcgctgga cccggaatca gcgcgcatcg
    26521 tgatggacac cctgcgcgac atcaaccaga acgacggcat caccgtggtc gtcacgctgc
    26581 atcaggtgga ttacgccctg cgctactgcg aacgcatcgt cgccctgcgt caggggcacg
    26641 tcttctacga cggcagcagc caactgtttg ataacgaacg ttttgaccat ctctaccgca
    26701 gcattaaccg catcgaagag aacgcgaaag ctgcctgata tccccatcat tgaggaaaac
    26761 gaatgaacgc taagataatt gcctcgctgg ccttcaccag catgttcagc ctcagcaccc
    26821 tgttaacccc ggcacacgcc gaagagcagg aaaaggcgct gaatttcggc attatttcaa
    26881 cggaatcaca gcaaaacctg aaaccgcaat ggacgccgtt tttacaggat atggagaaga
    26941 aactgggcgt gaaggtcaac gccttctttg ccccggacta cgcgggcatt atccagggga
    27001 tgcgcttcaa taaagtggat attgcctggt acggcaacct gtcggcgatg gaagcggtgg
    27061 atcgcgccaa tggccaggtc ttcgcccaga cggtcgcggc ggatggatcg ccgggttact
    27121 ggagcgtgtt gatcgtcaac aaagacagcc cgatcaacaa cctgaacgat ctgctggcga
    27181 agcggaaaga tctcaccttc ggcaatggcg atcctaactc cacctctggc ttcctcgtcc
    27241 ccggttacta cgtcttcgct aaaaacaata tctccgccag cgacttcaag cgcaccgtca
    27301 acgccgggca tgaaaccaac gcgctggccg tcgccaacaa gcaagtggat gttgccacca
    27361 acaacaccga aaacctcgac aagctgaaaa cctccgcgcc ggagaagctt aaagagctga
    27421 aggtgatctg gaaatcgccg ctgatcctag gcgatccgat cgtctggcgc aagaatctgt
    27481 ccgaaactac caaagacaag atctacgact tctttatgaa ctacggaaaa acgccggaag
    27541 agaaagcggt gctggaacgc ctgggctggg cgccgttccg cgcttccagc gacctgcaac
    27601 tggtgccgat tcgccagctc gcactgttta aagagataca aagcgtgaaa ggcaataaag
    27661 ggctgaatga gcaggacaag ctggcgaaaa ccaccgagat tcaggcgcag ctggatgacc
    27721 tggaccgcct gaacaacgcg ttaagcgcga tgagttcggt gagtaaagcg gtgcagtaaa
    27781 tcgtaggtcg gataagacgc cccggcgtcg catccgacaa tgtgtaggcg ttgatgccgg
    27841 atgcggtgca agcaccttat ccggcctaca gaccggagcc aaacatgcaa accatcacca
    27901 tcgccccacc caagcgtagc tggttctcgc ttctgagctg ggccgttgtt ctcgacgtgc
    27961 tggtcgtctc gtggcagggc gcggaaatgg ccccgcttac gctgattaaa gacggcggca
    28021 acatggcaac ct
//
LOCUS       DAJTYM010000052        27977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTYM010000052 DAJTYM010000000
VERSION     DAJTYM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            99..1130
                     /gene="mdtN"
                     /locus_tag="OO575_002234"
     CDS             99..1130
                     /gene="mdtN"
                     /locus_tag="OO575_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6665184.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1130..3172
                     /gene="mdtO"
                     /locus_tag="OO575_002235"
     CDS             1130..3172
                     /gene="mdtO"
                     /locus_tag="OO575_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6665185.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3169..4635
                     /gene="mdtP"
                     /locus_tag="OO575_002236"
     CDS             3169..4635
                     /gene="mdtP"
                     /locus_tag="OO575_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6665186.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4833..5072
                     /locus_tag="OO575_002237"
     CDS             4833..5072
                     /locus_tag="OO575_002237"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665187.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5082..5252
                     /locus_tag="OO575_002238"
     CDS             5082..5252
                     /locus_tag="OO575_002238"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6665188.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5301..6713
                     /gene="fdhF"
                     /locus_tag="OO575_002239"
     CDS             5301..6713
                     /gene="fdhF"
                     /locus_tag="OO575_002239"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6665189.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6714..6980
                     /locus_tag="OO575_002240"
     CDS             6714..6980
                     /locus_tag="OO575_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR6665190.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7074..7763
                     /locus_tag="OO575_002241"
     CDS             7074..7763
                     /locus_tag="OO575_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6665191.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7841..9154)
                     /gene="gltP"
                     /locus_tag="OO575_002242"
     CDS             complement(7841..9154)
                     /gene="gltP"
                     /locus_tag="OO575_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6665192.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9496..10092)
                     /gene="nrfG"
                     /locus_tag="OO575_002243"
     CDS             complement(9496..10092)
                     /gene="nrfG"
                     /locus_tag="OO575_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6665193.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10089..10472)
                     /gene="nrfF"
                     /locus_tag="OO575_002244"
     CDS             complement(10089..10472)
                     /gene="nrfF"
                     /locus_tag="OO575_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6665194.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10465..12183)
                     /locus_tag="OO575_002245"
     CDS             complement(10465..12183)
                     /locus_tag="OO575_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6665195.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12203..13159)
                     /gene="nrfD"
                     /locus_tag="OO575_002246"
     CDS             complement(12203..13159)
                     /gene="nrfD"
                     /locus_tag="OO575_002246"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6665196.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13156..13827)
                     /gene="nrfC"
                     /locus_tag="OO575_002247"
     CDS             complement(13156..13827)
                     /gene="nrfC"
                     /locus_tag="OO575_002247"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6665197.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13824..14390)
                     /gene="nrfB"
                     /locus_tag="OO575_002248"
     CDS             complement(13824..14390)
                     /gene="nrfB"
                     /locus_tag="OO575_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6665198.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14435..15871)
                     /gene="nrfA"
                     /locus_tag="OO575_002249"
     CDS             complement(14435..15871)
                     /gene="nrfA"
                     /locus_tag="OO575_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6665199.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16264..18222
                     /gene="acs"
                     /locus_tag="OO575_002250"
                     /pseudo
     CDS             16264..18222
                     /gene="acs"
                     /locus_tag="OO575_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18422..18736
                     /locus_tag="OO575_002251"
     CDS             18422..18736
                     /locus_tag="OO575_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6665200.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18733..20382
                     /gene="actP"
                     /locus_tag="OO575_002252"
     CDS             18733..20382
                     /gene="actP"
                     /locus_tag="OO575_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6665201.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20560..21852
                     /locus_tag="OO575_002253"
     CDS             20560..21852
                     /locus_tag="OO575_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6665202.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22006..23655)
                     /locus_tag="OO575_002254"
     CDS             complement(22006..23655)
                     /locus_tag="OO575_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6665203.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23806..25155)
                     /gene="ghxP"
                     /locus_tag="OO575_002255"
                     /gene_synonym="yjcD"
     CDS             complement(23806..25155)
                     /gene="ghxP"
                     /locus_tag="OO575_002255"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6665204.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25861..26325)
                     /gene="soxR"
                     /locus_tag="OO575_002256"
     CDS             complement(25861..26325)
                     /gene="soxR"
                     /locus_tag="OO575_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6665205.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26411..26734
                     /gene="soxS"
                     /locus_tag="OO575_002257"
     CDS             26411..26734
                     /gene="soxS"
                     /locus_tag="OO575_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6665206.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26737..27977)
                     /gene="pdeC"
                     /locus_tag="OO575_002258"
                     /pseudo
     CDS             complement(26737..27977)
                     /gene="pdeC"
                     /locus_tag="OO575_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
BASE COUNT         6985 a         7537 c         7112 g         6343 t
ORIGIN      
        1 tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac gccatgctgt
       61 tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg ccgaaaaaag
      121 cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt gcccttgttt
      181 tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg tcagcagata
      241 ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc accgacaacc
      301 aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac gaagccaacc
      361 tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc acccagcgta
      421 gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa aaagcccgtg
      481 ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg aaagagggtt
      541 ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca gaagcggatc
      601 ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc gtcgatgcgc
      661 tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta catctggaaa
      721 tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc tccgtcgggc
      781 aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac tggtacgtga
      841 tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc gcaacgattc
      901 gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt ggctacggcg
      961 tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct cgttctatta
     1021 actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa cctgacccgg
     1081 aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa tgagcgcgct
     1141 caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag agttaagcga
     1201 gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc tgctggtgat
     1261 tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag tgctgtttta
     1321 cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg tggttgccac
     1381 ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg aaccgttgat
     1441 ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc gcacccatcg
     1501 gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg ccatgctcga
     1561 ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc tctatccaac
     1621 cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta cgcaaatgca
     1681 tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca gcctcgcacc
     1741 gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga atgtcttttg
     1801 tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct gcgtggcaac
     1861 ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg ctgattctca
     1921 ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc agcatgccgt
     1981 tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag cggtggcggc
     2041 acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc agatgaaccc
     2101 gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg cttttaccaa
     2161 tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct gttacacctt
     2221 ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg tgatcgtcgc
     2281 taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg gggccttttg
     2341 cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg acaatattgt
     2401 cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg ccaccagctc
     2461 tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg ccacgctcga
     2521 aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc tgggtatcat
     2581 cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa gtgaagcgcg
     2641 cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa tgcggatccc
     2701 acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg gtctgcatgc
     2761 ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc aactggacag
     2821 cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg gccgcgatat
     2881 ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg cactacagcc
     2941 ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc tggcaaccgc
     3001 actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg ccatcctgcc
     3061 caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc cagactggac
     3121 agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat gatcaatcgt
     3181 caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat ttccggctgt
     3241 gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca aatcaaactg
     3301 gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg gaaacaactc
     3361 aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca caccctcgcc
     3421 gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga tgccggttca
     3481 cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc gaacggcttt
     3541 ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta ctatacggaa
     3601 gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca tcgctcagcg
     3661 gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc agtagagcta
     3721 tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta tcagatgctc
     3781 gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca ccaaagtaaa
     3841 gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca gattctggcg
     3901 gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga atctctgcgc
     3961 gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc attaccacga
     4021 gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg cccggatctg
     4081 caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc gcgggcgttg
     4141 ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca cctggatacc
     4201 ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt gccgctgttt
     4261 gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa catgatgatt
     4321 gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa cggcacgcgt
     4381 ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc aacgcgcttc
     4441 acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt acaggccact
     4501 gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga cagccgccgg
     4561 gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc ggctcccgtc
     4621 gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt cgtttcgtca
     4681 cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt gcccgtaata
     4741 tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt cgagagcgcg
     4801 tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg gtttgcccct
     4861 attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc gtccgggcgg
     4921 aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat tatggctggg
     4981 atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg atccgtcgcc
     5041 agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac gttgccgagc
     5101 gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc ggctcctcgc
     5161 gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc gttattggta
     5221 ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca ggtctgcacc
     5281 aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat accgatttag
     5341 tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat cacgtaatta
     5401 acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt gaaaccgcgc
     5461 gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg ttgaatgcga
     5521 tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct tcccgtacag
     5581 aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg gttgaagata
     5641 tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc caggcgaaaa
     5701 gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg gaaaccgtgc
     5761 gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat gcgggtgtaa
     5821 acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg ctgccggata
     5881 cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc gccaaagcct
     5941 ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg ccgcaccgcg
     6001 cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg caaactgatg
     6061 cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc gttcaggaca
     6121 tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg tcgtggggcg
     6181 agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc aaagcagttg
     6241 aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc acccgtatgg
     6301 gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt catctgtgcc
     6361 cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt cagtggcctt
     6421 gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag aagtttgata
     6481 ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc gacaaactca
     6541 ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac tcttgccgtt
     6601 cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc tacgcacaaa
     6661 tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt tgagtgcact
     6721 cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac aaaggggcga
     6781 tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc gaaagcttaa
     6841 gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag ccgatctccg
     6901 atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa actcgcctgc
     6961 gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag gctgtttttt
     7021 tatccattcg aactctttat actggttact ccctacccaa tcgtattatc aaaatgaaaa
     7081 aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac tccgagcctg
     7141 gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat tttcttgccg
     7201 acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag aaagccgccg
     7261 acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc aatccggtca
     7321 gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg ggcagtaaag
     7381 aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa ccggattatc
     7441 caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac tccgccgtcg
     7501 atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag gccgacgatg
     7561 acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt tattccgagt
     7621 actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag aacaaacaaa
     7681 aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg tgtgaagagt
     7741 ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg tcgcatcagg
     7801 catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc gcagttttat
     7861 caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca aatttgtgtt
     7921 cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc gcagtacgcg
     7981 ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc ggaataccta
     8041 cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca gcaatccctt
     8101 tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac aggtcaatgc
     8161 catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa ccatcaaggt
     8221 taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct ccgtaggctt
     8281 ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag tacgccagaa
     8341 tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac agacgtgcca
     8401 caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc agcaccagtt
     8461 tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca atcagcgcaa
     8521 acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg gtttcagaga
     8581 tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa gaaagcccca
     8641 gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc atcgacgcca
     8701 caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa ctgctttgta
     8761 ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc gacatatcca
     8821 ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg gcgacggtgg
     8881 tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt ttggcatcac
     8941 caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca atcattttga
     9001 tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc cagtcgcggc
     9061 tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata gcaaacagaa
     9121 tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca atggagcgcc
     9181 gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt gtgttgcgtt
     9241 ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg ttgcgcaacc
     9301 ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta tggcaggatt
     9361 aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat aatactgatt
     9421 ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa tgacataatc
     9481 ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat taatcgactc
     9541 gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt gccatagttc
     9601 gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca tcagagcggt
     9661 gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct gggcggtcat
     9721 atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt cagcgttctc
     9781 accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat cgttttgcca
     9841 cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag cgcggatttt
     9901 atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact gatgtagcgg
     9961 atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta acagataact
    10021 gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca ccggtatttg
    10081 aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag caataacaac
    10141 accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt atagcgaaca
    10201 aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt cccctccgac
    10261 accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga ttcgagtaag
    10321 ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc ctgttgctgt
    10381 tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa acaggtaaat
    10441 aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc atccgcttaa
    10501 caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac cgctttgtac
    10561 atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg cataccagtc
    10621 atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg cttcataaaa
    10681 acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg ccactatcgc
    10741 tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac ggaaggtgta
    10801 tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct gacgactgac
    10861 actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta ccagcgccgg
    10921 aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca gcatcaacag
    10981 accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc gtccccagcc
    11041 cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta gcactgcgca
    11101 aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa aacgcaccgc
    11161 cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc taatcagtgc
    11221 aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat gaaccgaaac
    11281 cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag taactatcgc
    11341 cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca aactgtgcag
    11401 cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat cccagaacca
    11461 ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga tccccgccgt
    11521 taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg ccgcatcaaa
    11581 ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac cgccatagcc
    11641 aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg gattgagatc
    11701 gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca gcaccacaaa
    11761 cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa atagcggatc
    11821 gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca gcagcaccca
    11881 aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt cccacgacag
    11941 caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga gaaaacaata
    12001 cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc cgatgcaagt
    12061 gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca acggggtcaa
    12121 cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa ggaatacgtc
    12181 cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag agaatgaaaa
    12241 agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg gcaggaatgc
    12301 cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc agcccggcga
    12361 caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc agcgcacgaa
    12421 ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg aagatttcac
    12481 cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa tgcgggttac
    12541 tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag atgccggaga
    12601 agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc agcgccgaca
    12661 gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc gtttccagtc
    12721 cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc ttcggcaacc
    12781 agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac agcaccagca
    12841 ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta aaactgtagt
    12901 ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg atcaacagac
    12961 cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta ctgtctgcac
    13021 cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga ccggcagaaa
    13081 taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat tcaaaatgaa
    13141 atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc ggtacagctt
    13201 cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca gcagttgcga
    13261 aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg ggcaagcttc
    13321 aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat cgcatttgtc
    13381 cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg cgatgcagta
    13441 ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg catcacgaaa
    13501 agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct ggcaagactt
    13561 acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac gaataatcgt
    13621 caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca tacaggcggt
    13681 acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat tgatattcaa
    13741 cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc cgacgccggt
    13801 gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac agacgccggg
    13861 ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt aatccgtcct
    13921 ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca gctggcgcac
    13981 gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg ttccggcaga
    14041 tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc gttaaagcgc
    14101 atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca gttggtgcag
    14161 gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg cataccttcg
    14221 gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg ggtaacttcg
    14281 tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag gctccacaac
    14341 aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat attcactcca
    14401 ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga ccgtttttac
    14461 gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc tcggccttga
    14521 tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag atatccggga
    14581 tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg gtgcgcgcat
    14641 ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt gcgtgcatat
    14701 gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg atatcgtcct
    14761 gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc gcttcgaagt
    14821 gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat tgcttacgtt
    14881 ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca caagtctggg
    14941 cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct tcggcgttct
    15001 gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga atgcccgctg
    15061 tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac agggagttag
    15121 tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact ttcatgccgt
    15181 catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc acatggcact
    15241 gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt ttaccaatgg
    15301 cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg cctttggcga
    15361 actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg atttccgggc
    15421 cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg atcagacgcg
    15481 ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct tcagcgtttt
    15541 tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca tgtccacgag
    15601 gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc cgtggatctt
    15661 ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag agatattgat
    15721 cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt tttgcgggag
    15781 ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat aagctgaaga
    15841 tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg cgccattgtt
    15901 gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt catgacattg
    15961 ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc aattgtaagt
    16021 gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac agcatgcata
    16081 actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa attaccctgc
    16141 cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa acaaaactgc
    16201 caatacccct acatttaacg cttatgccac atattattaa catcctacaa ggagaacaaa
    16261 agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg ttgcctgata
    16321 aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga taccttctgg
    16381 ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa aaatacctcc
    16441 tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa tctggcggca
    16501 aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat ctgggaaggc
    16561 gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga cgtctgccgc
    16621 ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc gatttatatg
    16681 ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg cgcgatgcat
    16741 tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat tgattccaac
    16801 tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat tccgctgaag
    16861 aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca tgtggtggta
    16921 ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg gtggcacgac
    16981 ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga agatccgctg
    17041 tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca cactaccggc
    17101 ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca tccgggtgat
    17161 atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt gctgtacggc
    17221 ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg gccgacgcct
    17281 gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac cgcacccacg
    17341 gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga ccgttcgtcg
    17401 ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga gtggtactgg
    17461 aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac cgaaaccggc
    17521 ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc ggcaacacgt
    17581 ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct ggaaggggct
    17641 accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac gctgtttggc
    17701 gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta tttcagcggc
    17761 gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt ggacgacgtg
    17821 ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct ggtggcgcat
    17881 ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg tcaggcgatc
    17941 tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta cgcagaagtc
    18001 cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct gcactggacc
    18061 gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg caaaattgcg
    18121 gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg cgtggtcgag
    18181 aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat tgccggatgc
    18241 gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag gcattgattg
    18301 ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc cgagcgttaa
    18361 ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg gagaatctgt
    18421 gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg agttagtcga
    18481 aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt atatcggctt
    18541 tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc cgaacaccag
    18601 cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg ttctcaccgg
    18661 tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag tcctgcatga
    18721 ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc tttcgcagct
    18781 aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg gcaggcgatt
    18841 attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc atcaaaacgc
    18901 gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt ccagaacggg
    18961 ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc cgcgctggtg
    19021 tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg ctggccaatc
    19081 attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc tgccgatgtg
    19141 gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg ttctctggta
    19201 gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat cgagctgctg
    19261 tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat gatgtacgtc
    19321 ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt gctgttgctg
    19381 ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag cttcaacaat
    19441 ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa accgggcgga
    19501 ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt tggtacggcg
    19561 ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga agcacgtaag
    19621 agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt tattatcggc
    19681 ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc gggccatctg
    19741 attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg caacctattc
    19801 ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc ggatctgacg
    19861 ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa aaaaggcgcg
    19921 accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg cgtgattgcg
    19981 attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg ccttgcgttt
    20041 gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg gtcgaaactg
    20101 actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt ggtgctgatg
    20161 atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat cttcccgtat
    20221 gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt cttctcggca
    20281 accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca gtttatccgc
    20341 tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc cttccccggt
    20401 cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca taatattaaa
    20461 caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga taatgcagag
    20521 aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa tggtttaaat
    20581 aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag ttcatcacat
    20641 acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt aattgacaat
    20701 aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt gattcagaaa
    20761 atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac ggacaggaaa
    20821 atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga cgatagaatt
    20881 acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt atattttata
    20941 aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa tggtggttac
    21001 gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt atccaattcg
    21061 aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat gtataaaacg
    21121 aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct cgatgactca
    21181 aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc tatgcatgtt
    21241 caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct tgactattca
    21301 aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc gccatatgtt
    21361 aatttattta aagccgacct tagtttctct aaactcgatt taattaactt tgaacatgct
    21421 gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt aattgatagc
    21481 aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg taccggctct
    21541 aatatggcta atgttaattt taataatgcc aatttaagca actgccattt caactgttct
    21601 attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt cgatgaggct
    21661 agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc cattgatagt
    21721 gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca tactggcatg
    21781 tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga tattatgcaa
    21841 cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa gagagataac
    21901 ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt gcaaaagatt
    21961 gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact gattttcctc
    22021 aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg tttcgttgct
    22081 gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca atgccgccag
    22141 acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt tacgtccatc
    22201 ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca actggttatc
    22261 gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg ccacaatcgc
    22321 cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg aatggtccgc
    22381 gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca gcgagaagag
    22441 aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt taccatccgg
    22501 caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca ccccagcgaa
    22561 agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct tcttcttcag
    22621 gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca gcatcagcgc
    22681 cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag ggtcgatttc
    22741 tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca gcaggaacac
    22801 catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca ggcgcattgc
    22861 cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg caaccgcagc
    22921 gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg gcagcaagaa
    22981 cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga atcgcagcga
    23041 gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg ctactttcat
    23101 aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca ctgccacggc
    23161 aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct gcaaaatgcc
    23221 catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca cagcatcggt
    23281 cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg gcaccaccca
    23341 gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg cgaggccgaa
    23401 aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat cagcgaacag
    23461 caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat gcaaaccaaa
    23521 cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg ggatctgaaa
    23581 gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta tcagtatggt
    23641 gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag aaagacgtta
    23701 tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc ggagaaaata
    23761 aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga tagcccagcc
    23821 acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt tcaacttgcg
    23881 ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca tgatgcctgt
    23941 tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca tcgcatcaac
    24001 aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa cgtacatcag
    24061 cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga acagaatcag
    24121 caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg ccgccgtacc
    24181 cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag agaaaacgct
    24241 gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat ctttatccag
    24301 caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata ctgccgtcat
    24361 caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa tgtccaggct
    24421 gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa cgccggagaa
    24481 atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg tcagcagaat
    24541 gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg ccagtgacat
    24601 aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa gcgggttttt
    24661 aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga taccgatccc
    24721 cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga tacccgtggc
    24781 agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga caggtacgct
    24841 aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga tggcgcaacc
    24901 aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc cggcaaccag
    24961 acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca tacctggaac
    25021 gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca cttcctgacg
    25081 gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc cggtacgcgc
    25141 tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa gatgcgtggc
    25201 ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc aatttggtgc
    25261 gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa aaaaagcaaa
    25321 cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt ttctctttta
    25381 ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg atgtgcaaga
    25441 ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt ctgagatgac
    25501 aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg gtgacataca
    25561 attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt gtgttgacgt
    25621 cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc ggaaaacaaa
    25681 ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa agagctcgat
    25741 cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag caccagtgca
    25801 aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc ttgtggcgct
    25861 ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta agcggtcgcc
    25921 cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac atccgtccag
    25981 ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc gccattggga
    26041 cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca cgccaaacgc
    26101 ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa ttgcgacata
    26161 tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac tagtaatcaa
    26221 ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt tcgccacttc
    26281 gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc gctttacctc
    26341 aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa cactgaaaag
    26401 aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga ttgacgtgca
    26461 tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt caaagtggta
    26521 cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca ttcgccaacg
    26581 ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt ttgatatcgc
    26641 aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc ggcagtttga
    26701 tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg ttaactcccg
    26761 cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct tcgcaaaaaa
    26821 ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct cctcagtttc
    26881 gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga tgtgttccgc
    26941 aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat cgattttcaa
    27001 aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac caaaatcatc
    27061 aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc attttttcga
    27121 catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt
    27181 gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca
    27241 ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga tcgcggaaga
    27301 cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa
    27361 taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac aacgcttcag
    27421 cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat
    27481 gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta
    27541 gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat
    27601 gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc
    27661 gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt aaatccttca
    27721 aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt
    27781 tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc
    27841 agaagagagg gttgataacc gccacataat ttccccgctg catataggtc attttatagc
    27901 cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat
    27961 cggccggtgc aatcgta
//
LOCUS       DAJTYM010000053        26942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTYM010000053 DAJTYM010000000
VERSION     DAJTYM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..410
                     /locus_tag="OO575_002259"
     CDS             <1..410
                     /locus_tag="OO575_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR6665207.1"
                     /translation="MVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQRHLHP
                     QINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMFKGHIN
                     TGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            401..874
                     /locus_tag="OO575_002260"
     CDS             401..874
                     /locus_tag="OO575_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6665208.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            981..1520
                     /gene="dnaT"
                     /locus_tag="OO575_002261"
     CDS             981..1520
                     /gene="dnaT"
                     /locus_tag="OO575_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6665209.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            1523..2260
                     /gene="dnaC"
                     /locus_tag="OO575_002262"
     CDS             1523..2260
                     /gene="dnaC"
                     /locus_tag="OO575_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6665210.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2309..2803
                     /locus_tag="OO575_002263"
     CDS             2309..2803
                     /locus_tag="OO575_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6665211.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3043..5334
                     /gene="opgB"
                     /locus_tag="OO575_002264"
     CDS             3043..5334
                     /gene="opgB"
                     /locus_tag="OO575_002264"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6665212.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(5472..6494)
                     /gene="lgoD"
                     /locus_tag="OO575_002265"
                     /pseudo
     CDS             complement(5472..6494)
                     /gene="lgoD"
                     /locus_tag="OO575_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            6633..7547
                     /locus_tag="OO575_002266"
     CDS             6633..7547
                     /locus_tag="OO575_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6665213.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            7762..9123
                     /locus_tag="OO575_002267"
     CDS             7762..9123
                     /locus_tag="OO575_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6665214.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9172..10836)
                     /gene="tsr"
                     /locus_tag="OO575_002268"
     CDS             complement(9172..10836)
                     /gene="tsr"
                     /locus_tag="OO575_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6665215.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(10955..11401)
                     /gene="hpaR"
                     /locus_tag="OO575_002269"
     CDS             complement(10955..11401)
                     /gene="hpaR"
                     /locus_tag="OO575_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6665216.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            11673..12962
                     /locus_tag="OO575_002270"
     CDS             11673..12962
                     /locus_tag="OO575_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6665217.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            12959..14425
                     /gene="hpaE"
                     /locus_tag="OO575_002271"
     CDS             12959..14425
                     /gene="hpaE"
                     /locus_tag="OO575_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6665218.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            14427..15278
                     /gene="hpaD"
                     /locus_tag="OO575_002272"
     CDS             14427..15278
                     /gene="hpaD"
                     /locus_tag="OO575_002272"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6665219.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15288..15668
                     /locus_tag="OO575_002273"
     CDS             15288..15668
                     /locus_tag="OO575_002273"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6665220.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            15736..16539
                     /gene="hpaH"
                     /locus_tag="OO575_002274"
     CDS             15736..16539
                     /gene="hpaH"
                     /locus_tag="OO575_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6665221.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            16550..17353
                     /gene="hpaI"
                     /locus_tag="OO575_002275"
     CDS             16550..17353
                     /gene="hpaI"
                     /locus_tag="OO575_002275"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6665222.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            17375..18730
                     /gene="hpaX"
                     /locus_tag="OO575_002276"
     CDS             17375..18730
                     /gene="hpaX"
                     /locus_tag="OO575_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6665223.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            18740..19630
                     /gene="hpaA"
                     /locus_tag="OO575_002277"
     CDS             18740..19630
                     /gene="hpaA"
                     /locus_tag="OO575_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6665224.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            19657..20594
                     /locus_tag="OO575_002278"
                     /pseudo
     CDS             19657..20594
                     /locus_tag="OO575_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            20821..22379
                     /gene="hpaB"
                     /locus_tag="OO575_002279"
                     /pseudo
     CDS             20821..22379
                     /gene="hpaB"
                     /locus_tag="OO575_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            22397..22909
                     /locus_tag="OO575_002280"
     CDS             22397..22909
                     /locus_tag="OO575_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6665225.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23302..25452
                     /gene="btsT"
                     /locus_tag="OO575_002281"
     CDS             23302..25452
                     /gene="btsT"
                     /locus_tag="OO575_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6665226.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            25502..25705
                     /locus_tag="OO575_002282"
     CDS             25502..25705
                     /locus_tag="OO575_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6665227.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            25716..26672
                     /gene="yjiA"
                     /locus_tag="OO575_002283"
     CDS             25716..26672
                     /gene="yjiA"
                     /locus_tag="OO575_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6665228.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(26741..>26942)
                     /locus_tag="OO575_002284"
     CDS             complement(26741..>26942)
                     /locus_tag="OO575_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6665229.1"
                     /translation="TLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKDVCNSSEW
                     GLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6487 a         7129 c         6867 g         6459 t
ORIGIN      
        1 taatggttgg cctttcttgc gcctgtttct gtaaactcaa taacggtggc tgggatggtg
       61 ccgtcatcac cttctttgcc agtacgaccg cgatgtatat ccgccagctg ctggcacaac
      121 gtcatcttca tccacagatc aacttttgcc ttaccgcttt cgccgccacc accatttccg
      181 gattgctttt gcaactcccc actttcagca atacccccac cattgcgatg gccgccagcg
      241 ttctgctgct agtgccgggc tttccgttga ttaatgccgt cgccgatatg tttaaaggcc
      301 acatcaatac cggactggca cgctgggcga tcgccagtct gctgacactg gctacctgcg
      361 tcggcgtagt gatggcactg acgatttggg ggctacgcgg atgggtgtga tcgaatttct
      421 gttagcgttg gcgcaggata tgatcctcgc cgccattcct gcggtcggct ttgcgatggt
      481 gttcaacgtt cccgtgcggg cgttacgctg gtgtgcgctg cttggctcga taggtcatgg
      541 ttcccgaatg atcttgatga ccagcgggtt gaatattgag tggtcaacct ttatggcttc
      601 tatgctggtc ggtaccattg gtattcaatg gtcgcgctgg tatctggcgc atccgaaagt
      661 gtttaccgtg gcggccgtta tccctatgtt cccgggcata tcggcttata ccgcaatgat
      721 ttcgtcggta aaaatcagcc agttaggtta cagcgaaccg ttgatgatta ccctgttaac
      781 caactttctt acagcttcat cgattgttgg tgcgttatcc atcggtcttt ccattcctgg
      841 attatggttg taccgcaagc gccctcgcgt ataaaattgc ctcgcttaag cacacggatg
      901 agagacagcc tcctctcctc cgtgtgttac tataaaagtt atctcccttc tcgttcatcg
      961 ttccatattt gagaaacagt atgtcttcca gagttttgac cccggacgtc gttggtattg
     1021 acgccctggt acacgatcac caaaccgttc tggcaaaagc tgaaggcggt gtggttgccg
     1081 tatttgctaa caatgccccg gcgttttatg ccgtcacgcc tgcacgcctg gctgaactgc
     1141 tggcgctgga agaaaagcta gcgcgtccgg gaagcgatgt cgctctggac gatcaactct
     1201 atcaggaacc gcaagccgct cccgttgctg tacccatggg gaaattcgcc atgtatccgg
     1261 actggcaacc cgatgccgat tttatccgcc tggcggcgct atggggcgtg gcgctaagag
     1321 agccggtgac caccgaagaa ctggcctcat tcattgccta ctggcaggcg gaaggtaaag
     1381 tctttcacca tgtgcagtgg caacaaaaac tggcgcgcag cctgcaaatc ggtcgtgcca
     1441 gcaacggcgg actgccgaaa cgagatgtga atacggtcag tgaacctgac agccaaattc
     1501 caccaggatt cagagggtaa cgatgaaaaa cgttggcgac ctgatgcaac gcctgcaaaa
     1561 aatgatgcct gcccatatca aacctgcatt caaaacgggt gaagagctac tggcctggca
     1621 gaaagaacaa ggggcgatcc gctccgccgc tctcgaacgt gaaaatcggg cgatgaaaat
     1681 gcagcgcacc tttaaccgct ccggtattcg tccactgcat cagaactgct cctttgagaa
     1741 ctatcgcgtt gagtgtgaag ggcagatgaa tgcgttaagc aaagcgcgcc agtatgtcga
     1801 agagtttgac ggcaacatcg ccagcttcat cttttctggt aagccaggaa ccggcaaaaa
     1861 ccatctggcg gcggcaatct gcaacgagct gctgctacgc ggtaaatccg tattgatcat
     1921 caccgtggcc gatattatgt cggcgatgaa agataccttc aggaatagcg gtaccagcga
     1981 agaacaactg cttaacgatc tgagcaacgt tgatctgctg gtgatcgatg agatcggcgt
     2041 gcagaccgaa tcgaaatacg aaaaagtgat catcaaccag atcgtcgatc gccgctcttc
     2101 ttccaaacgc ccaaccggga tgctgaccaa cagtaatatg gaagagatga ccaagttgct
     2161 gggcgaacgc gtgatggacc gtatgcgcct gggtaacagt ttatgggtga tcttcaactg
     2221 ggatagctac cgtagccggg taacgggtaa agagtattaa tccccgtttc tggcggtata
     2281 ctgcgacaca attccacctt gagtaatgat gatgaaaact attaaacatc ttctgtgctg
     2341 tgccattgcc gccagcgcat taatttccac cggggtgcat gctgcgtcct ggaaagatgc
     2401 gctctccagc gccgccagcg aacttggcaa ccaaaacagc acgacacagg aaggcggttg
     2461 gtcgctcgcg tcattaacta acttgcttag cagcggaaac caagccttaa gcgcagataa
     2521 catgaacaat gccgcaggca ttctgcaata ctgcgcgaag caaaagctgg cttcggtaac
     2581 cgatgccgaa aacatcaaga accaggtgct ggaaaagctg ggcctgaaca gtgaagagca
     2641 aaaagaagac accaactatc tggacggtat tcagggtttg ctgaaaacaa aagatggtca
     2701 gcaactcaat ctggataaca tcggaacgac tccgctggca gaaaaggtga aaaccaaagc
     2761 ctgcgatctg gtgttaaagc aggggctgaa cttcatttcc tgaaccgcga aatgttaccc
     2821 ggctgacgcg gctctctcct gcccttttcc ctgctcctgg acggttttcc ccctgttaca
     2881 cgggaagcga ccgcgatcaa aaaagtctgt ttataaacta attcctaaca acatcgcatt
     2941 tttttgacac tagaattgca gcaatatggc gacgccatta cattgtgctt ctcaaaaagc
     3001 ttaaacgttg gctaaacggc ccggtttagt gaggatcgcc cattgtcaga actactctct
     3061 ttcgcccttt ttctcgcctc tgtgctgatt tacgcatgga aagcgggacg taacacctgg
     3121 tggtttgcag ccacgttaac ggtgctgggg ctatttgtcg ttttaaatat caccctgttt
     3181 gccagcgact attttactgg cgatggtatt aacgacgcgg ttctctatac cttaaccaac
     3241 agcctgaccg gtgctggcgt cagcaaatac atcctgccgg gtatcggcat tgtgctgggg
     3301 ctgacggcgg tgttcggtgc gctgggctgg atcctgcgcc gtcgtcgtca tcatccgcac
     3361 cattttggtt acagcctgct ggcgctctta ctggcgctgg gttcggtgga cgccagcccg
     3421 gcatttcgtc agataacgga actggtgaaa tcccagtcac gcgatggcga cccggacttt
     3481 gcggcttatt ataaagagcc gtcgaaaact atccctgacc cgaaactcaa cctggtttat
     3541 atctacggcg aaagtctcga gcggacctat tttgataacg aagctttccc ggatctcacg
     3601 cctgaactgg gcgcgttgaa aaatgaaggc ctggatttca gccacacgca gcagctgcca
     3661 ggaacggatt acacgattgc gggcatggtg gcttctcagt gcggcatacc gctgttcgcc
     3721 ccctttgaag gcaacgcctc cgcctctgtc tccagcttct tcccgcagaa catctgtctg
     3781 ggcgatatcc tgaaaaactc gggttatcag aactatttcg tgcagggcgc aaatctgcgt
     3841 tttgccggta aagatgtgtt cctgaaatcg cacggcttcg accacttata cggctcagaa
     3901 gagctgaaaa gcgtggtagc cgacccgcac tatcgcaacg actggggatt ctacgacgat
     3961 accgttctcg atgaagcgtg gaaaaagttt gaagagcttt cccgctcagg tcagcgattc
     4021 tcactgttta ccctgacagt cgatacccat cacccggatg gttttatctc gcgcacctgt
     4081 aaccgcaaaa aatatgattt tgacggtaag ccgaatcagt cattcagcgc ggtaagttgc
     4141 agccaggaga atatcgcgac gtttatcaac aaaatcaaag cgtcaccgtg gtttaaagat
     4201 actgtcatcg tcgtctcttc tgaccattta gcgatgaaca acacggcgtg gaaatacctc
     4261 aataagcagg atcgcaataa cctgtttttt gtcattcgtg gcgacaagcc gcagcaaaag
     4321 acgctggcag tgaagcgtaa cacgatggat aacggcgcga cggtgctgga cattctcggt
     4381 ggcgataact atctcggact tggtcgtagc agtttatccg ggcagtcgat gtcggaaatc
     4441 ttcctcaata tcaaagagaa aacattggcg tggaagccag atatcatccg cctgtggaaa
     4501 ttccctaaag agatgaaaga gttcaccatc gaccagcaga aaaacatgat tgccttctcg
     4561 ggtagccatt tccgtttgcc gctgctgttg cgggtttcag acaaacgcgt ggaaccgctg
     4621 ccggaaagcg aatactcagc accgctgcgt ttccagctgg ccgatttcgc tccacgcgac
     4681 aatttcgtct gggttgacag ttgctacaag atggcacaac tctgggctcc ggaactggca
     4741 ctttccaccg actggtgtgt ctcgcagggg cagcttggcg gtcagcaaat tgttcagcat
     4801 gttgacaaaa caacatggaa gagcaagacg gcatttaaag atacggtcat cgacatggcg
     4861 cgttacaaag gcaatgtcga tacgctgaag attgttgata acgatattcg ctacaaagcc
     4921 gacagtttca tctttaacgt cgccggtgcg ccagaagagg tgaaacagtt tagcgggatt
     4981 tcccgtccgg agtcgtgggg ccgctggtcc aacgcgcagc tgggcgatga agtaaaaatc
     5041 gagtacaagc atccgctgcc gaagaaattt gacctggtga ttaccgccaa agcatacggc
     5101 aataacgcca gccgtcctat tccggtacgc gtaggcaatg aagaacaaac tcttgtgctg
     5161 ggcaatgaag tgaccaccac cacactgcat ttcgataacc caaccgatgc cgacacactg
     5221 gtaattgtgc cgccggaacc tgtctcaacc aacgaaggga atatcctcgg acactcgccg
     5281 cgtaagctcg ggatcggcat ggtggaaatt aaagtggtag aacgtgaagg gtaattcctg
     5341 actgatgtgt aggtcgcata gggtgcgacc tacacgcctt ctccggctta caacacatta
     5401 ttaagaaaca aaaaaaacag cgcccaacac ttccccggac gctgcatggc aactttaata
     5461 atctcgcctt atcagaaagt aattacaccc ttaatcaact cacgattgtt aatcacatcg
     5521 cgctcgtagg tttctgccag cgtggcgaac ggatagcgat gggttaacat catgtcggca
     5581 gtgattttcc cttccgccat cagtcgaccg actttagcga aatcttccgg cgtggcgttg
     5641 cggctgccca tcatcgtcgt ttctttttta tggaattccg gatcggagaa ctgcaactca
     5701 cctttaaaca gaccgacaaa taccaccgtg ccgccgtgac gaatcagatt cacggtatta
     5761 ttcatcgcat gttgattacc tgtcgcgtcg atcactttct gcgccagcga accaccaaac
     5821 tgcgcccgca gctgcgcgtc aaaatcttcg gctgacgggt ccagcacggg caactccaga
     5881 cgcgttgcca catgttcacg gcgcgccgga ctggtatccg ccaccaccac ctgtgcgcca
     5941 tcggctttag cgattgccgc cgcgcccaga ccgattggcc ccgccccgac caccagcacc
     6001 tgctcgccgg gagcaatggc tgcgcgacgc accgcatgag cgctaatagc gaaaggttca
     6061 atcaatgcgg ccgcctgcgg gtcaataccg tctgcgggca aaatgttcgc caccggcacg
     6121 ctcaaatact cactaaaacc gccatcctga tgcacgccaa tgactgaaat tttttcacag
     6181 cagttggtac gcccgctttt acacgccggg cattgctgac aggcaacata agggatcaca
     6241 gcaacttgct gaccattttt aagatcagca atatttttac ccagcccaac aatctcccca
     6301 catatttcat ggcctaaaac ccgtggataa ctaaaaaatg gttgatttcc accctaggca
     6361 tgaatatcgg taccgcaaat cccgacagac ttaattttta ttaatgcttc attgtcaccc
     6421 ggaataggta tctcgcgttg tttccagact aattctttcg gctgctggca aattaaaaca
     6481 ttcatcgtag acatgattcg gctatccatt ttttgttgcc ttagatatca atgaaaatga
     6541 aaaatggcag cggaacggaa aatctgtttt gtgaaaacac acgcataaaa atgttttaaa
     6601 tcgggtttta atggactcac aaggaggaaa atatgagtcg ttcacaaaat ttacgccaca
     6661 atgtgattaa ccaggtcatt gatgatatgg cgcggggtca tatcccttcc ccacttccat
     6721 cgcaaagtgc gctggcggaa atgtacaaca tcagccgcac tacagtgcgt catattctca
     6781 gccaattacg cgaatgcggc gtcttaacgc aggttggaaa cgactatgtt attgcgcgta
     6841 aacctgacca tgatgacggt ttcgcctgta ctaccgcttc aatgagtgaa caaaacaaag
     6901 tgtttgagca ggcttttttc acgatgataa accagcgtca gttacgccct agggaaactt
     6961 tttctgaact gcaactggct cgggccgcgg gtgtcagccc ggtggttgta agagaatatc
     7021 ttttaaaatt cggccgttat aatcttattc aaagcgaaaa gcgcggccag tggagcatga
     7081 agcaattcga tcaatcctac gccgagcagc tgtttgagct acgcgaaatg ctcgaaacac
     7141 attcacttca gcactttctc aacctgcctg atcatgatcc ccgctggctg caggcgaaaa
     7201 ccatgctaga acgtcatcgg ctactgcgtg acaacatcgg caatagcttt cgcatgttct
     7261 cgcaacttga cagagatttt cactccttac tgctttcagc tgccgacaat atcttttttg
     7321 atcaatcgct tgagatcatc tcagtcatct ttcattttca ctatcaatgg gacgaaagcg
     7381 atcttaaaca gcgcaatatc attgccgtcg atgaacatat gaccattctc agcgctctga
     7441 tttgccgcag tgaccttgat gccacccttg cgctccgtaa ccatttgaat tcagctaaac
     7501 aatcaatgat tcgatccatt aatgagaaca cgcgttatgc tcattaagta cggattaaaa
     7561 acagcaaact attagcgggt gcacggtttt atcaaaagat aaataccggg aaattcccgc
     7621 ttacctatgc tcacaatcag tcgactaaaa atgtttttgt gagcggtagt aaagtcctaa
     7681 aactttaacc tgccgttaat gtcaaccttg cgtaatgcac aattacgtaa acaattaaaa
     7741 ttataaatac aggagtccgg cgtggaaaaa gaaaatatca ccctcgatcc gcgttcttca
     7801 tttactccat cttcgtcggc agatattccc gtgccaccag atggattagt tcaacgcagt
     7861 acccgaatta aacgcattca aaccaccgcc atgttgttat tattttttgc ggcggtaatc
     7921 aattatctcg accgcagttc gctgtcggta gcaaatttaa cgattcgtga agaattggga
     7981 ttaagtgcca ccgaaatcgg cgctttgctc tccgtgtttt cactcgctta cggcattgcg
     8041 caacttcctt gcggcccact attggatcgt aaaggcccac gcctgatgct gggactgggg
     8101 atgttcttct ggtcactgtt ccaggccatg tctggcatgg tgcacagctt tacgcagttc
     8161 gtgttggtgc gtatcggtat ggggattggt gaagcgccga tgaacccatg cggtgtaaaa
     8221 gtcattaacg actggttcaa catcaaagag cgcggacgcc cgatgggctt cttcaacgca
     8281 gcttctacca ttggcgttgc tgtaagccca ccgattctgg cggcaatgat gctggtgatg
     8341 ggctggcgcg ggatgtttat taccattggt gtactgggga tttttctcgc catcggctgg
     8401 tatatgctct atcgcaaccg cgagcacgta gaactgactg ccgttgaaca agcttatctc
     8461 aatgcaggta gcgtcaatgc ccgccgagat ccgctcaatt ttgccgaatg gcgcagcctg
     8521 ttccgtaacc gcacaatgtg gggaatgatg ctcggattca gtggcatcaa ctacactgcg
     8581 tggctgtatc tggcctggct tcctggttac ctgcaaacag cctataacct ggatttaaaa
     8641 agcacagggt tgatggcggc tatccctttc ctgtttgggg ctgccgggat gctggtcaac
     8701 ggttacgtta ctgactggct ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc
     8761 tgcattatta ccgggatgtt ctgttctgcc gcctttacgc tgatagtacc acaagcgaca
     8821 acatccatga tggcggttct gctgattggc atggcactgt tctgtattca ctttgccgga
     8881 acatcctgct ggggcttgat ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc
     8941 agtatccaga actttgccag cttcatctgc gcctctttcg cgccgatcat tactggtttt
     9001 attgttgata caacccactc attccgtctg gcactaatca tttgcggttg cgtcaccgca
     9061 gcgggagcac tggcgtacat cttcctggtt cgccagccga tcaacgaccc gcgtaaagat
     9121 taaactccct atgcccgata acattttgct tatcgggcat ttttatggcg attaaaacgt
     9181 ttcccagttc tccccgctat ctgccactgc cattttacgc ggcgtagctg gcgtcacggt
     9241 ttttaccaca gccgatgttt cacgctgctg ttgctgctgt tgctgaatcc ggaacactgc
     9301 cacagcttcg gtcaggcgac tggcctgctc ttccagcgcg gcggcggcag cggcagattc
     9361 ttccaccagc gcggcgttct gttgagttac ccggtccatc tcagcaaccg ctaagccaac
     9421 ctgatcgata ccacggctct gctcatcaga agcagaagct atttcgccca taatgtccgt
     9481 cacgcgggtc acagcactga caatctccgc cattgtttcc ccggcgcttt cgaccagcgt
     9541 agagccaaca tccactttcc ccaccgagtc ttcaatcagg cttttaattt cacgaaccgc
     9601 ctgggcgcta cgctgggcca gattacgcac ttctcccgca accaccgcaa aaccacgtcc
     9661 ttgctcaccc gcacgcgctg cttcaaccgc cgcgttcaaa gccagaatat tggtctggaa
     9721 ggtaatgccg tcaattacgc tgataatatc ggcgattttc tgcgaactgg tggagatatc
     9781 gcgcatagtc tgcaccacgt tatctaccac tttgccgccg cgttgcgccg tttcagaagc
     9841 acttaatgcc agatggctgg cctggcgcgc attctcggcg ttctgtttca ccgttgcggt
     9901 cagttgctcc atgctggctg ccgtctcttc cagcgaagcg gcctgttgct cggtgcgcga
     9961 agagagatca ttattgccgg tagcgatttc gctggcaccg ctatagatgg cattggcccc
    10021 gttgcgcaca tcaccgacgg tacgcatcag ctctccctgc atatggcgca aactctctgc
    10081 cagttgcccc atctcattag agccatccac ctcaatcggt ttcaccagat cgccgcctgc
    10141 aatatgacga atgctgtcaa tcaggcgatt cattggcgct accagcgagg ctttaatacc
    10201 gaaccagacg gcgaagatga ccgccagtac gacgatcatc acgcccacca gaatccacat
    10261 cgcctggctg taggaggcat tgttatcgct gacggcgata tcatagagcc gatcgttttg
    10321 ctccatgtaa gccacatact gcttctcgaa accgtcctga tatccctggg tcggctgatc
    10381 aaagaactcg ttgattttgc ctgcacctaa cagttggatc agctccgcca gcgcattgtg
    10441 ataaatatcg taattacgtt tgatctctgc cgctgcggcg gtgctctgac gcgggtcacg
    10501 cggcaacgct tcgtaatccg cccagttttt ttccgcctgt ttcagcgaaa tactggcact
    10561 ctgcatcagc tcagcaacgg ttgaaccgct accaatatta ttctgatcca tcatgtagcg
    10621 gatacccgcg cggttgaggg tgttacgcgt ctgcaacaac gcgacccagc tgccattcag
    10681 cgtgggttgc tgctggcgaa tggtttgtaa aacagtgaaa ttttctttgt cattctttaa
    10741 ggcattaaaa aacagaccgc ctgatgtcag ttgtaaaagg ccaaaaacgg ccaaaaccag
    10801 cagtaagctg gtcacgatct tgatacgttt taacatggtt tctctttcct gtggaccgat
    10861 gcaagatttt cggcctggaa agcaaaaact ttatgaaaag gtgtaacccg ccatccatgg
    10921 aaaaaccagg cgctggttat cagatactaa aaagttattc attatcgccc gggatgtctt
    10981 cttgtcggga attgcccaga gcaataaatt cctctaacag atgggttaac tgttgcattt
    11041 tctcggcagt aaactgagct tcaatctgcc gataggcttc ttcaatctgt gtctgagcgc
    11101 ggttatacag cgcctgcccc tctttggtca gcgagatata gagcttccgc tgatcattga
    11161 tcggctttaa tcgcaacact aaaccgtcgc gttccatccg cgtgaggatc ccggtcaggc
    11221 ttgggcgcaa aatgcaggcg cgatacgcca gatcgtgaaa atccattgat gggctttccg
    11281 ccagaatacg cacgatgcgc cactgttgct cagtcaaatt atgccgttta acgattgggc
    11341 gaaaataact catcgccgct tctcgcgcct ggagcaacgc aatggttagt gagtcgtgca
    11401 ttatctttcc cctgaaaaat tcaaaatcat taatatagaa acaattaatc atcacgttac
    11461 cgggactgag ctgcccccgc aattcttcat tttatcaaaa ttgatgatat tttattaact
    11521 tactgatatt taatatttta ttatctttaa atgtaaatag tttgttaatt agatcacatt
    11581 tatatcactt acttttgcca aactacgcaa tcgggaataa aagtaatcat taacatatta
    11641 atgattaaga tgatcgaatc cgaggagtgg taatgaaagg cactatcttc gccgtagcgt
    11701 tgaaccatcg cagccaactt gatgcctggc aggaagcgtt ccagcaatct ccctacaaag
    11761 ccccgcctaa aactgcggtc tggtttatta aaccgcgcaa tacggtgatt ggttgcggtg
    11821 aaccgattcc ctttccacag ggtgaaaaag tactgagcgg tgcgactgtt gcgctgattg
    11881 tggggaaaac ggcgacgaaa gtacgtgaag aagatgcggc agagtacatc gccggatatg
    11941 cgctggataa cgacgtcagc ctgccggaag agagctttta ccgtccggca atcaaagcaa
    12001 aatgtcgtga tggattctgc cccattggcg aaaccgtggc tctcagcaat gtcgataatc
    12061 tgactatcta taccgagatc aacgggcgtc ctgccgatca ctggaatacc gccgatttac
    12121 aacgtaacgc cgcacagctg ctgagcgccc tgagcgaatt tgccacgctg aatccaggcg
    12181 atgctattct gctcggcacg ccacaggcgc gcgtggaaat acagccaagc gatcgcgtgc
    12241 gtgttctcgc agaaggtttc ccgccgctgg aaaatccggt agtggacgaa cgtgaagtga
    12301 ccacgcgcaa gagcttccca acgctgccac acccgcacgg tacgctgttt gccctcggcc
    12361 tgaactacgc cgaccacgcc agcgaactgg aatttaagcc accggaagag ccgctggtgt
    12421 tcctgaaagc gccgaatacc ctcactggcg acaaccagac ctccgtgcgc ccgaacaata
    12481 ttgaatacat gcactacgaa gcggagctgg tggtagttat tggcaagcag gcgcgtaacg
    12541 tcagcgaagc cgatgccatg gattatgtcg cgggctacac cgtgtgtaac gactacgcca
    12601 ttcgcgacta tctggaaaac tactaccgcc ctaacctgcg ggtcaaaagc cgcgacggac
    12661 tgacgccgat gctttcaacc atcgtgccga aagaggcgat cccggacccg cataatctga
    12721 cccttcgcac cttcgtcaac ggcgagttac gccagcaagg caccaccgcc gatctgatct
    12781 tcagcgtgcc cttcctgatc gcctacttaa gcgaatttat gaccctgaat ccgggcgaca
    12841 tgatcgccac cggcacacca aaaggcttat ctgacgtggt gcctggcgat gaagtagtgg
    12901 tggaagtaga aggcgtgggc cgcctggtga accgaattgt gagtgaggaa acagcgaaat
    12961 gaaaaaagta aatcattgga tcaacggcaa aaatgttgca ggtaacgact acttccagac
    13021 taccaatccg gcaacgggtg aagtgctggc ggatgtggcc tctggcggtg aagcggagat
    13081 caatcaggcg gtagcggcag cgaaagaggc gttcccgaaa tgggctgatc tgccgatgaa
    13141 agagcgtgcg cgcctgatgc gccgtctggg cgatctgatc gaccagaacg tgcctgagat
    13201 cgccgcgatg gaaaccgcgg acacgggcct gccgatccat cagaccaaaa atgtgttgat
    13261 cccacgcgct tctcacaact ttgaattttt cgcggaagtc tgccagcaga tgaacggcaa
    13321 gacctatccg gttgacgaca agatgctcaa ctacacgctg gtgcaaccgg taggcgtttg
    13381 tgcgctggcg tcgccgtgga acgtgccgtt tatgaccgcc acctggaagg tcgcgccgtg
    13441 tctggcgctg ggcaataccg cggtgctgaa gatgtcggaa ctctccccgc tgaccgccga
    13501 ccgcctgggt gagctggcgc tggaagctgg tattccggcg ggcgtgctaa acgtggtaca
    13561 gggctacggc gcaaccgcag gcgatgcgct ggtccgtcat catgacgtgc gtgccgtgtc
    13621 gttcaccggc ggtaccgcca ccgggcgcaa catcatgaaa aacgccgggc tgaaaaaata
    13681 ctccatggag ctgggcggta aatcaccggt gctgattttt gaagatgccg atattgagcg
    13741 tgcgctggac gccgccctgt tcaccatctt ctcaatcaac ggcgaacgct gcaccgccgg
    13801 ttcgcgcatc tttattcagc agagcatcta tccagaattc gttaaacgct ttgccgaacg
    13861 cgccaaccgt ctgcgcgtgg gcgatccgaa cgatccgaat acccaggtta gggcgcttat
    13921 cagtcagcaa cactgggaaa aagtctccgg ctatatccgt ctcggcattg aagaaggcgc
    13981 aaccctgttg gcgggcggcc cggataaacc gtccgacctg cctgcgcacc tgaaaggcgg
    14041 caacttcctg cgcccaacgg tgctggcgga cgtagataac cgtatgcgcg ttgcccagga
    14101 agagattttc gggccggtcg cctgcctgct gccgtttaaa gacgaagccg aaggcttacg
    14161 cctggcaaac gacgtggagt acggcctcgc gtcgtacatc tggacacagg atgtcagcaa
    14221 agtgttacgc ctggcgcgtg gcattgaagc aggcatggtg ttcgtcaaca cccagaacgt
    14281 gcgcgacctg cgccagccat ttggcggcgt aaaagcctcc ggcaccgggc gtgaaggcgg
    14341 tgagtacagc ttcgaagtgt tcgcggaaat gaagaacgtc tgcatttcca tgggcgacca
    14401 tccaattccg aaatggggag tctgatatgg gtaagttagc gttagccgcc aagatcacgc
    14461 acgtaccgtc gatgtatctc tctgaactac caggaaaaaa ccacggttgt cgccagggtg
    14521 cgatcgacgg gcataaagag atcagcaagc gttgccggga aatgggcgtc gataccatta
    14581 tcgttttcga tactcactgg ctggtcaaca gtgcttatca catcaactgt gcagaccatt
    14641 ttgaaggcgt ctacaccagc aacgagctgc cgcattttat tcgcgacatg acctacaact
    14701 acgagggcaa cccggagttg gggcagctaa ttgccgagga agccttaaag ctcggcgtgc
    14761 gggcaaaagc gcacaatatt cccagcctga aactggaata cggcacgctg gtaccgatgc
    14821 gctacatgaa tgaagacaag cacttcaaag tggtctccat ttcggctttc tgcacggttc
    14881 acgattttgc cgacagccgc aagctgggcg aagcgattct gaaagcgatc gaacagtacg
    14941 acggcaccgt ggcggtcctt gccagcggtt cgttatcgca ccgctttatt gacgatcagc
    15001 gtgcggaaga agggatgaac agctacaccc gcgagttcga ccgccagatg gacgagcgtg
    15061 tggtgaagct gtggcgcgaa ggccagttca aagagttttg caatatgctg ccggagtacg
    15121 ccgactactg ctacggcgaa ggcaatatgc acgacacggt gatgctgctg gggatgctcg
    15181 gctgggataa atacgacggc aaggtggagt ttattaccga gctgttccca agctctggca
    15241 ccggtcaggt taacgctgtt ttcccgctgc ccgcgtaagg acgttttatg ccgcacttta
    15301 tcgtggaatg tactgaaaat attcgtgaag aagcccgctt acctgagctg tttgccagtg
    15361 ttaacactgc actggcggca acggggattt ttccgcttgg gggaatacgt agccgggcgc
    15421 actggatcga tacctggcaa atggctgatg gcaagcacga ttacgccttt gtgcatatga
    15481 cgctaaaaat cggcagcgga cgcagtctgg aaagccgtca ggaagtgggc gaaatgctgt
    15541 ttgatctgat taaaacgcac ttcgcctcac tgatggagag ccgttaccta gcgctttcat
    15601 ttgagattgc agagctgcac ccgacgttga atttcaaaca gaacaacgta cacgcgttgt
    15661 ttaaataaat ttgcaccagc ttccaggccg gatgataagg cgctgccgca tccggcaatg
    15721 ccacaggata tcgctatgtt cgataaacac acccacaccc tgatcgccca gcgtctggat
    15781 caggcagaaa aacagcgcga acagatccgc gcgatctcgc tggattaccc ggagatcacc
    15841 atcgaagacg cctacgccgt gcagcgcgaa tgggttcgcc tgaaaatcgc cgaaagtcgc
    15901 acgctgaaag gccacaaaat cggtctgacc tcgaaagcga tgcaggcaag ctcgcagatc
    15961 agcgaaccgg attacggtgc actgctggac gacatgttct tccacgatgg cagcgatatc
    16021 ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc tggcttttgt gctggcaaaa
    16081 ccgctgcgtg ggccaaactg cacgctgttc gacgtttaca acgccacgga ctatgtgatc
    16141 ccggcgctgg agctgatcga cgctcgctgc cacaacatcg atccggaaac ccagcgcccg
    16201 cgcaaagtat tcgacaccat ttccgataac gccgccaacg ccggagtgat cctcggaggt
    16261 cgtccgatca aaccagacga actggatctg cgttggatct ccgccctgat gtatcgcaat
    16321 ggcgtgattg aagaaaccgg cgtcgccgct ggtgtgctga atcatccggc aaacggcgtg
    16381 gcctggctgg cgaacaaact cgcaccgtat gacgtacaac tggaaaccgg gcaaatcatt
    16441 ctcggtggtt cgttcaccct cccggttccg gcgcgtaagg gcgacacctt ccacgtcgat
    16501 tacggcaaca tgggctccat tagctgccgc tttgtttaag gagagaacga tggaaaacag
    16561 ttttaaagcg gcgctgaaag caggccgtcc gcagattgga ttatggctgg ggctgagcag
    16621 cagctacagc gcggagttac tggccggacc aggattcgac tggttattga tcgacggtga
    16681 gcacgcgccg aataacgtgc aaaccgtgct cacccaacta caggcgattg cgccctaccc
    16741 cagccagcca gtggtacgcc catcgtggaa cgatccggtg caaatcaaac aactgctgga
    16801 cgtaggcaca caaaccttac tggtgccgat ggtacaaaac gccgacgaag cccgtaaagc
    16861 ggtacgcgcc acccgttatc cccccgccgg tattcgcggt gtgggcagtg cgctggctcg
    16921 cgcctcgcgc tggaatcgca ttcctgatta cctgcaaaaa gccaacgatc aaatgtgcgt
    16981 gctggtgcag atcgaaacgc gtgaggcaat gaagaactta ccgcagattc tggacgtgga
    17041 aggggtcgac ggcgtgttta tcggcccggc ggatctgagc gccgatatgg gttatgccgg
    17101 taatccgcag cacccggaag tacaggccgc cattgagcag gcgatcgtgc agatccgcga
    17161 agcgggcaaa gcgccgggga tcctgatcgc caatgagcaa ctggcaaaac gctatctgga
    17221 actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc ctgctcgccc gcgccgccga
    17281 agcgctggca gcacggtttg gtaatagttc ttctatttcg atggcgaagc aaaacaacaa
    17341 ctctgtctac taaacgtacg atgtggagcg catcatgacg acctctaccc tgcaatataa
    17401 agataataaa actgttgaaa ttgaacatcg tgtcattaat aaattatttc gtcgcctaat
    17461 catttttctt tttattctgt ttgttttttc gtttcttgat cgcatcaata taggatttgc
    17521 cggactgaca atgggcaagg atcttggcct gacgtccaca atgtttggcc tcgccgcaac
    17581 actcttttat gtcacgtacg tcatgtgcgg gatccccagt aatatgatgt tagcgaaaat
    17641 tggcgctcgt cgatggatcg ctgggatcat ggtggtctgg ggaattgctt ccacctgcac
    17701 aatgtttgca acaagccccg aaacactcta tctactgcgt atgctggttg gtattgctga
    17761 ggcgggtttt ttacctggta ttttggttta tctgacctgg tggttcccgg catatcatcg
    17821 ggccagagcc aatgccttat ttatgatagc gatgccagtc actatgatgc tgggattgat
    17881 cctttcaggc tacattctgg ccttggatgg actgtggaat ctaaaaggct ggcaatggct
    17941 ctttttgctg gaaggtctgc catcggtcat tcttggtgtc gtcacctggt tttatctcaa
    18001 cgacacacca gaccaggcga cctggcttga tgaaaacgaa aaacaggctt taaaaacgat
    18061 gattctccgt gaacaagaga gtgcatttgc ccatgcatct gttccacgct caaccttacg
    18121 tgaagtcctg accccagcca tcatgctata tacacttgct tatttttgcc cgactaatac
    18181 tctgagtgcg attaatatct ggacgccgca aatcctacag agtttcaata ctggcagtag
    18241 caacatcatg attggtttgc tggcggccat cccccaattc tgcacaattt ttggcatgat
    18301 ctggtggagt cgtcgttccg accgtcttaa agaacgcaaa aagcatacga ttcttcccta
    18361 cctgtttgcc gctgccggat ggttactcgc ttctgcgaca gatcacagcc tcattcaact
    18421 gagcagcatc attatggcct caatgggatc atttaccgcg atgactatct tttggacaac
    18481 accggatcaa gttataagct tgcaatcgcg tgccgttgcg atggctgtca tcaatgctgt
    18541 cggcaatgta ggttcagcag tcagtccatt acttatcggt attttgaaag atgcaaccgg
    18601 cagttttagc tcaggtttgt ggtttgtagc aggattgctg gtactgggcg cgttagtcat
    18661 gacccgaatc cctatgggga gtcgggaaca ctctctttca tctgaactta tcgcaccaaa
    18721 accgcaataa ggagtcatga tgtccagtga agctatcttt gaacatggtt atgaaaatat
    18781 tgatttggtc cgggaatatg atgcccgcta cgctggtgaa gatgtgcatt atgaaacatt
    18841 tgcccggtta gcgaatttct ttggacgcga tatgcgccct cactggcacg atagatattt
    18901 tcaactccat tatctggtca ctggacgcat tactttacaa ctggatgagc acttctattc
    18961 tgtgcgcgca ccgctttttg tactaacacc accatcggtg ccacatactt tctttactga
    19021 tcctgatagc gatgggcatg ttctgaccgt acgtcaggag ttaatttggc ccctggtaga
    19081 aaagctctgg cctggtagtg gagatgccgc aatgctacaa ggtatctgtc tgtcactgga
    19141 gggaatggaa catacactgg cggcattaaa tcattattgg ccgataatat ccctggagtt
    19201 tcaacaaaat aagcaaggac gggaaattct attgcaatca ctggcgcaaa gtatttttac
    19261 aatcctactg cgtgaagcac caccaaatga tatttcaaca tgcagcgtgc gcggcgagat
    19321 gtgtttattc cagaagttta atcggctgat tgacgaaaat tatcgccaac atttaatggt
    19381 ccctgaatat gcaacaatgc tcggtatgag tgaatcacgg cttaccgagc tgtgccgacg
    19441 ttttgccaat caatcgccga agagattgat tttcgaacga gtgagccggg aagccagacg
    19501 gcttctgctc tatagcgagc aaagcatcaa ccagatagcc cttgatcttg gctacaaaga
    19561 tcccgcctat ttcgcccgtt ttttcaaccg aatgatgggt tgctcaccaa cacaattccg
    19621 tggtcgctga ccatttatca acctgagaga ctcgacatga aaatatcttt acgctcacta
    19681 tttgtggcgt gcctgattgc gccagtggca ttcacctacg cctccgaacc gcaataaatg
    19741 aaaattgtac tacttggaac gaaaggtggc ccttctttat taaaagctga ccgtttaccg
    19801 atggcaacgg cacttattat tggcaataaa acctggttaa ttgatgctgg ttacggggcc
    19861 agcttacaac tggtaaaaaa taatattcca ctgcgccaga ttgataatat tttactcacc
    19921 catttacaca gtgatcatat tctggactat ccatcagtct taatgaatgc atgggctagc
    19981 ggtctgcagg acaaaactat tcatgtattc ggtccgccag gcacccgagc catgaccgct
    20041 gccagttggc aggtcttcga tcgggacatc acactgcgta tggaagaaga aggaaaaccc
    20101 gatcctcgca aactcgttac agctcaagat gttggtgaag gcgtagttta taaagatgat
    20161 accgtgaccg tgactgcatt aaaggtccct cattctcctt tccctgacgg tgaggctttt
    20221 gcttatcgct tcgacacaca aggcaagcga attgtcttct caggagatac ctcgtacttt
    20281 cccccactgg caactttctc agaaggcgct gatatacttg tgcatgaggc cgtacatgag
    20341 ccctccgtcg ctaagctggc agacagtatt ggcaatggta aaacactggc aaaagccatt
    20401 gcttctcacc atacgactat tgaggatgta ggcaaaattg ccaatgaagc aaaagtgaaa
    20461 aaactcatcc ttagccacct ggtaccaatc accgttccgg atgaaacatg gaagcaagaa
    20521 gcacagaaaa ctattcaggt cctatcgtcg ttggtcatga aaatatgacg attaccattc
    20581 cattcgcccc ataaaaaatt ccgtccccgt tagcccttgt tagcggggaa tattatgatt
    20641 tgccttactg taagttgatc acgatcgcag tttactattt aactccacct tgctgtcgaa
    20701 aagtaccaac gttcgcctga aacgtctctt cccgacaacc gcgttcaacg cctcaattaa
    20761 ataacaaaaa caacacacaa aattaacaat cgtattccga ttaatactgt agaggtcgac
    20821 atgaaaccag aagatttccg cgccagtacc caacgtcctt tcaccgggga agagtatctg
    20881 aaaagcctgc aggatggtcg cgagatctat atctatggcg agcgagtgaa agacgtcacc
    20941 actcatccgg catttcgtaa tgcggcagcg tctgttgccc aactgtacga cgcgctgcac
    21001 aaaccggaga tgcaggactc tctgtgttgg aacaccgaca ccggcagcgg cggctatacc
    21061 cataaattct tccgcgtggc gaaaagtgcc gacgacctgc gccagcaacg cgacgccatc
    21121 gctgaatggt cacgcctgag ctatggctgg atgggccgta ccccagacta caaagccgct
    21181 ttcggttgcg cactgggcgc gaatccgggc ttttacggtc agttcgagca gaacgcccgt
    21241 aactggtata cccgtattca ggaaactggc ctctacttta accacgcgat tgttaaccca
    21301 ccgatcgtca tttgccgacc gataaagtga aagacgttta catcaagctg gaaaaagaga
    21361 ctgacgccgg gattatcgtc agcggtgcga aagtggttgc caccaactcg gcgctgactc
    21421 actacaacat gattggcttc ggctcggcac aagtgatggg cgaaaacccg gacttcgcac
    21481 tgatgttcgt tgcgccaatg gatgccgatg gcgtgaaatt aatctcccgc gcctcttatg
    21541 agatggtcgc gggtgctacc ggctcgccat acgactaccc gctctccagc cgcttcgatg
    21601 agaacgatgc gattctggtg atggataacg tactgatccc atgggaaaac gtgctgatct
    21661 accgcgattt tgatcgctgc cgtcgctgga cgatggaagg cggtttcgcc cgtatgtatc
    21721 cgctgcaagc ctgtgtgcgc ctggcagtga aactcgactt cattacggca ctgctgaaaa
    21781 aatcactcga atgtaccggc accctggagt tccgtggtgt gcaggccgat ctcggtgaag
    21841 tggtggcgtg gcgcaacacc ttctgggcat tgagtgactc gatgtgttca gaagcgacgc
    21901 cgtgggtcaa cggggcttat ttaccggatc atgccgcact gcaaacctat cgcgtactgg
    21961 caccaatggc ctacgcgaag atcaaaaaca ttatagaacg caacgttacc agtggcctga
    22021 tctacctccc ttccagtgcc cgtgacctga acaatccgca gatcgaccag tatctggcga
    22081 agtatgtgcg cggttcgaac ggtatggatc acgtccagcg catcaagatc ctcaaactga
    22141 tgtgggacgc cattggcagc gaatttggtg gtcgtcacga gctgtatgaa atcaactact
    22201 ccggtagcca ggatgagatt cgcctgcaat gtctgcgcca ggcacaaagc tccggcaata
    22261 tggacaagat gatggcgatg gttgatcgct gcctgtcgga atacgaccag aacggctgga
    22321 ctgtgccgca cctgcacaac aacgacgata tcaacatgct ggataagctg ctgaaataac
    22381 gcagcaggag gttaagatgc aattagatga acaacgcctg cgctttcatg acgcgatggc
    22441 cagcctgtcg gcagcggtaa atattatcac caccgagggc gacgccggac aatgcgggat
    22501 tacggcaacg gctgtctgct cggtcacgga tacaccaccg tcgctgatgg tgtgcattaa
    22561 cgccaacagt gcgatgaacc cggtttttca gggcaacggc aagttgtgcg tcaacgtcct
    22621 caaccatgag caggaactga tggcgcgcca cttcgcgggc atgacaggca tggcgatgga
    22681 agagcgtttt agcctctcat gctggcaaaa aggtccgctg gcgcagccgg tgctaaaagg
    22741 ttcgctggcc agtcttgaag gagagatccg cgatgtgcag gcgattggca cacatctggt
    22801 gtatctggtg gagattaaaa acatcatcct cagtgcagaa ggtcacggac ttatttactt
    22861 taaacgccgt ttccatccgg tgatgctgga aatggaagct gcgatttaag ctccagcccc
    22921 ttcacgcatt cgtcgaaggg gcatttttat tgtgatctca atcacatatc tacccgatga
    22981 ataattcttg cccaaccgct caccgccatt cctgaccgct tacatcccta aaataaccac
    23041 tcagttattt accttacttt acgcgcacgt atctctggca acatcactgc aggatagcgg
    23101 tcaatttacc tcctcaaacg caacgcaaac ctagaacggc ttcggcctat gattaacaaa
    23161 ttactctatg gattttggtt tgcaggaagg cagcaagtga gtgaatcccc gggagcttac
    23221 aaaagtaagt gtcagaggtg aacgaacgca gctgccgcac ctgtaagcca aaagacgacg
    23281 agtaaaatgc caggttttac tatggatacg aaaaaactat tcaagcacat accctgggtg
    23341 attctcggaa tcatcggtgc attctgcctc gcggtagttg cattacgtcg gggggagcac
    23401 gtcagcgccc tgtggatcgt ggtcgcctct gtgtcggtgt atctggtggc gtatcgctac
    23461 tacagtctgt acatcgccca gaaggtgatg aaactcgacc ccacgcgcgc gacgcctgcg
    23521 gttattaaca acgacggtct gaactacgtt ccgaccaacc gttacgtgtt gtttggtcac
    23581 cacttcgccg ctatcgccgg tgctggtccg ctggtgggac cggttctcgc cgcgcagatg
    23641 ggctacctgc ctggcacgct gtggctgctg gcgggggtag tgctggccgg tgcggtacag
    23701 gactttatgg tgctgtttat ctcctctcgc cgtaacggcg catctctcgg tgagatgatc
    23761 aaagaagaga tggggccagt accggggact atcgcgctgt ttggctgttt cttaatcatg
    23821 atcatcatcc tcgccgttct ggcgctgatt gtggttaaag ccctggccga aagtccgtgg
    23881 ggtgtcttca ccgtttgctc aaccgtaccg attgcgctgt ttatgggtat ctacatgcgc
    23941 tttatccgtc cggggcgtgt gggtgaagtc tctgttattg gtatcgtgct gctggttgcc
    24001 tctatctact tcggtggcgt gattgctcac gatccgtact ggggtccggc actgaccttt
    24061 aaagacacca ccattacctt cgcgctgatt ggctatgcgt ttgtttccgc actgctgccg
    24121 gtgtggctga tcctcgcacc gcgcgactat ctggcaacct tcctgaaaat cggcgttatc
    24181 gtcggcctgg cgctgggtat cgtggtgctg aacccggaac tgaaaatgcc tgcgatgacc
    24241 cagtacattg acggtactgg cccgctgtgg aaaggcgctc tgttcccgtt cctgttcatc
    24301 accatcgcct gtggtgcggt atctggcttc cacgcgctga tctcttccgg tacgacgccg
    24361 aaactgctgg ctaacgaaac cgacgcgcgt ttcattggct acggcgcaat gctgatggag
    24421 tccttcgtgg cgattatggc gctggttgca gcgtccatca tcgaaccggg tctttacttc
    24481 gcgatgaaca ccccgcctgc tggccttggc atcaccatgc ctaacctgca tgaaatgggt
    24541 ggcgagaacg cgccgatcat catggcgcag ctgaaagacg ttaccgcaca cgcggcagcg
    24601 accgtcagct cctggggctt cgtgatttcg ccagagcaga tcctgcaaac cgcgaaagac
    24661 attggtgagc cttctgtcct gaaccgtgca ggtggtgcgc cgacgctggc ggtaggtatc
    24721 gctcacgtgt tccacaaagt gctgccgatg gctgacatgg gcttctggta tcacttcggt
    24781 attctgttcg aagccctgtt catcctgacc gcgctggatg cgggtacccg ttctggccgc
    24841 tttatgctgc aagacctgct gggtaacttc atcccgttcc tgaaaaaaac cgactctctg
    24901 gttgccggta tcatcggtac tgcgggctgt gtgggtctgt ggggctacct gctgtatcag
    24961 ggcgtggtcg atccgctggg cggcgttaag agcctgtggc cgctgttcgg tatctccaac
    25021 cagatgctgg cagccgtagc gctggtactg ggcaccgttg tgctgattaa gatgaagcgc
    25081 acccaataca tctgggtaac tgttgttccg gctgtatggc tgcttatctg caccacctgg
    25141 gcgctgggtc tgaaactgtt cagcaccaac ccgcagatgg aaggcttctt ctacatggca
    25201 agccagtaca aagagaagat tgctaacggt actgacctga cggcgcagca gattgccaac
    25261 atgaaccaca tcgttgtgaa caactacacc aacgcaggcc tgagtattct gttcctgatt
    25321 gtggtgtaca gcatcatctt ctacggtttc aaaacctggc ttgcggtgcg taacagcgac
    25381 aaacgtactg acaaagaaac accgtacgtt ccaatcccgg aaggcggcgt gaagatctct
    25441 tcgcaccact aaccgtgttt agccccgctt cggcggggct ttgttcttat cagagtgaac
    25501 tatgtttggt aacttaggac aggcaaaaaa atatctcggc caggcggcga agatgttgat
    25561 tggtattcca gactacgaca actacgtcga gcatatgaag accaaccatc ccgacaagcc
    25621 gtacatgagc tatgaagaat tcttccgcga acgccagaac gcgcgctacg gcggcgatgg
    25681 taaaggcggt atgcgctgtt gttaatggag agagcatgaa cccgattgca gttaccctac
    25741 tcaccggttt tttaggcgcg ggcaaaacta ccctgctgcg ccatattctt aacgaacaac
    25801 acggctacaa gattgccgtg attgaaaacg aattcggcga agtctccgtt gatgaccaac
    25861 tgattggcga ccgcgccacg cagatcaaaa cgctgaccaa cggctgcatc tgctgttcgc
    25921 gctccaacga gctggaggac gcactactcg acctgctgga taatctcgac aagggcaata
    25981 ttcagttcga tcgtctggtg attgaatgca ccggcatggc cgatcccggc ccgattattc
    26041 agaccttttt ctcccatgag attttatgcc agctctacct gctggacggc gtgattgcgc
    26101 tggtggatgc ggtacatgcc gatgagcaga tgaaccagtt caccatcgcc cagtcccagg
    26161 tgggctacgc cgaccgtatt ctgctgacta aaaccgacgt cgcaggcgaa gcagagaaac
    26221 tgcgtgaacg cctggcgcgc atcaacgccc gcgcaccggt ctacaccgtc acccacggcg
    26281 acatcgacct gggcctgctg ttcaacacca acggttttat gctggaagag aacgtcgtca
    26341 gcaccaaacc gcgtttccac tttatcgcgg ataaacaaaa cgatatttcg tcaattgtgg
    26401 tggaactgga ttacccggta gatatcagcg aagtttcccg cgtgatggaa aacctgctgc
    26461 tggaatcggc ggataaactg ctgcgttaca aagggatgct gtggattgac ggcgaaccta
    26521 accgcctgct gttccagggc gtccagcgcc tctacagcgc cgactgggac aggccgtggg
    26581 gcgatgaaaa accgcatagc acaatggtgt ttatcgggat tcagttgccg gaggaagaga
    26641 ttcgggcggc gtttgcggga ttgaggaagt aagttcggga aagatcaact ttttgagagt
    26701 tccaccgcaa cattattacg agtggaactc tttagatatg tcagaccaac caaagcttct
    26761 tcaattgcgt tttcattttt tcatgtctca ttttcagttc agccaatccc cattctgaag
    26821 aattacaaac atcttttgtt aaataaaaag agcttttact attaagtttc tcataaatag
    26881 atttcttttt gataaaatca tagtgttgtg tttcagaatt tttggaacca cttatgagtg
    26941 tt
//
LOCUS       DAJTYM010000054        26916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTYM010000054 DAJTYM010000000
VERSION     DAJTYM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO575_002285"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO575_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6665230.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO575_002286"
     CDS             complement(1614..1937)
                     /locus_tag="OO575_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6665231.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO575_002287"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO575_002287"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6665232.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO575_002288"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO575_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6665233.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO575_002289"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO575_002289"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6665234.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO575_002290"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO575_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6665235.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO575_002291"
     CDS             7537..8574
                     /locus_tag="OO575_002291"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6665236.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO575_002292"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO575_002292"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6665237.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO575_002293"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO575_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6665238.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO575_002294"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO575_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6665239.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO575_002295"
     CDS             10745..11626
                     /locus_tag="OO575_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6665240.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO575_002296"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO575_002296"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6665241.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO575_002297"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO575_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6665242.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO575_002298"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO575_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO575_002299"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO575_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6665243.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO575_002300"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO575_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6665244.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO575_002301"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO575_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6665245.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO575_002302"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO575_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6665246.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO575_002303"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO575_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6665247.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO575_002304"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO575_002304"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6665248.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO575_002305"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO575_002305"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6665249.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO575_002306"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO575_002306"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6665250.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO575_002307"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO575_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6665251.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO575_002308"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO575_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6665252.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO575_002309"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO575_002309"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6665253.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO575_002310"
     CDS             24893..25273
                     /locus_tag="OO575_002310"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6665254.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO575_002311"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO575_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6665255.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO575_002312"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO575_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6665256.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..26916
                     /locus_tag="OO575_002313"
                     /pseudo
     CDS             26442..26916
                     /locus_tag="OO575_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6645 a         6693 c         7060 g         6518 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcgg
//
LOCUS       DAJTYM010000055        25839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTYM010000055 DAJTYM010000000
VERSION     DAJTYM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            299..592
                     /locus_tag="OO575_002314"
     CDS             299..592
                     /locus_tag="OO575_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6665257.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            664..1323
                     /locus_tag="OO575_002315"
     CDS             664..1323
                     /locus_tag="OO575_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6665258.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1415..1861
                     /locus_tag="OO575_002316"
     CDS             1415..1861
                     /locus_tag="OO575_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6665259.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2068..2968)
                     /gene="hlyE"
                     /locus_tag="OO575_002317"
                     /pseudo
     CDS             complement(2068..2968)
                     /gene="hlyE"
                     /locus_tag="OO575_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3341..3760
                     /locus_tag="OO575_002318"
     CDS             3341..3760
                     /locus_tag="OO575_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6665260.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3760..5028
                     /gene="umuC"
                     /locus_tag="OO575_002319"
     CDS             3760..5028
                     /gene="umuC"
                     /locus_tag="OO575_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6665261.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5074..5604)
                     /gene="dsbB"
                     /locus_tag="OO575_002320"
     CDS             complement(5074..5604)
                     /gene="dsbB"
                     /locus_tag="OO575_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6665262.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5750..7291)
                     /gene="nhaB"
                     /locus_tag="OO575_002321"
     CDS             complement(5750..7291)
                     /gene="nhaB"
                     /locus_tag="OO575_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6665263.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7512..8231
                     /gene="fadR"
                     /locus_tag="OO575_002322"
     CDS             7512..8231
                     /gene="fadR"
                     /locus_tag="OO575_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6665264.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8283..9815)
                     /locus_tag="OO575_002323"
     CDS             complement(8283..9815)
                     /locus_tag="OO575_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6665265.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10145..11443
                     /gene="dadA"
                     /locus_tag="OO575_002324"
     CDS             10145..11443
                     /gene="dadA"
                     /locus_tag="OO575_002324"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6665266.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11453..12523
                     /gene="dadX"
                     /locus_tag="OO575_002325"
     CDS             11453..12523
                     /gene="dadX"
                     /locus_tag="OO575_002325"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6665267.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12691..14105)
                     /locus_tag="OO575_002326"
                     /pseudo
     CDS             complement(12691..14105)
                     /locus_tag="OO575_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14716..16452)
                     /locus_tag="OO575_002327"
     CDS             complement(14716..16452)
                     /locus_tag="OO575_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6665268.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16547..17461)
                     /gene="ldcA"
                     /locus_tag="OO575_002328"
     CDS             complement(16547..17461)
                     /gene="ldcA"
                     /locus_tag="OO575_002328"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6665269.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17561..18172
                     /gene="emtA"
                     /locus_tag="OO575_002329"
     CDS             17561..18172
                     /gene="emtA"
                     /locus_tag="OO575_002329"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6665270.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18174..18908)
                     /locus_tag="OO575_002330"
     CDS             complement(18174..18908)
                     /locus_tag="OO575_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6665271.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19109..19363
                     /locus_tag="OO575_002331"
     CDS             19109..19363
                     /locus_tag="OO575_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6665272.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19413..20873)
                     /locus_tag="OO575_002332"
     CDS             complement(19413..20873)
                     /locus_tag="OO575_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6665273.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20810..21367)
                     /locus_tag="OO575_002333"
     CDS             complement(20810..21367)
                     /locus_tag="OO575_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6665274.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21393..22247)
                     /locus_tag="OO575_002334"
     CDS             complement(21393..22247)
                     /locus_tag="OO575_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6665275.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22247..23056)
                     /locus_tag="OO575_002335"
     CDS             complement(22247..23056)
                     /locus_tag="OO575_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6665276.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23066..23824)
                     /locus_tag="OO575_002336"
     CDS             complement(23066..23824)
                     /locus_tag="OO575_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6665277.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23821..24801)
                     /locus_tag="OO575_002337"
     CDS             complement(23821..24801)
                     /locus_tag="OO575_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6665278.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24801..25823)
                     /locus_tag="OO575_002338"
     CDS             complement(24801..25823)
                     /locus_tag="OO575_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6665279.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6438 a         6356 c         6717 g         6328 t
ORIGIN      
        1 cgtggaggtt agcagtgcaa gcagcaatac agccttactg atgctcttga ttttcattgg
       61 tagtctcctg ttcagaatga gtacgatgaa ctgttataat ataacaatcc cttacgggag
      121 aattccagtg gcttttctgc gttcggtaaa atcccggcgc ggcatttttt gccgggggga
      181 ttacttataa aggggcatgg actcaacaaa ccgcagaaat ctacagctgg ctggcttgtg
      241 gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga gtaaaagtat
      301 gttttgtgcg atttatcgaa gcagcaagcg tgaccagacc tatttatatg tcgaaaaaaa
      361 agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc ctcagttagc
      421 gatgattctg ccgttggatg ggcggaagaa actggtcaat gctgatattg aaaaggttaa
      481 actggcatta accgagcaag gttactattt gcagttaccg ccaccacccg aagatttgct
      541 gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat aaccgatatc
      601 cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg tgaggggaac
      661 gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt gagtaaagta
      721 gtctgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc agtgcccgaa
      781 gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca gccattggcg
      841 atcccatccg atttcggttc agttcatcat gaagtcgaac tggcggtgtt gattggcgcg
      901 acgctgcgtc aggcaacgga agagcatgtc cgcaaagcca ttgccggtta cggcgtggcg
      961 cttgatctga cattgcgtga tgttcaggga aaaatgaaga aagccgggca gccgtgggaa
     1021 aaggctaaag cgtttgataa ctcttgtccg ctttccgggt ttatccccgc ggcggaattt
     1081 accggcgatc cacaaaatac aacgctgggc ctgagcgtaa acggcgaaaa acgccagcaa
     1141 ggtacgactg cggacatgat ccataaaatc gttccgttga tcgcttatat gagcaagttt
     1201 tttaccctca aggccggtga cgttgtgttg acaggcacgc ctgatggcgt cggcccgttg
     1261 caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac tcgcgttttg
     1321 taatactttt tgccgccaga aagcggcggc aaaacttgca tcgctgtgcc agactggtta
     1381 taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt tctggcaaag
     1441 taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg gttgcggtca
     1501 gtgttgcttg cataaactga tggatgaaga caccgacgaa atctacttca ctaacgtcgc
     1561 ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc gtttcgagtt
     1621 tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat ggctgccaat
     1681 gacctgcgct tatcggttgc tggcggaagg taaagattta cctgcgtggc atccgctact
     1741 tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata tcgcagtgaa
     1801 agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact gggcgcagtg
     1861 ataaatttaa tagatgctga tgggggcttt cttaatatac gtctggatgc tcgccatttc
     1921 gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc ttattatcaa
     1981 caggtaactc tcaatcttta aaaaacgtct gtgtttttaa atgtgataaa ccttatacct
     2041 tgagtacatg gagagaataa tcgcttatca gacttcaggt acttcaaaga gtgtcttttt
     2101 accgtgtctt ttctgatact cattacaggt gttaatcatt tttttggccg ctgcttttag
     2161 caaagaaagc attaaatcat cataatcaac atagaatctg gttgtttcag tttccgtttt
     2221 tatctccccg atggcggcta tttcggtggt taatttcaat ttggcggcat cgatatcttt
     2281 attcgcttgt ttaaccgtgt tagacagggt ggtaaagaaa ctctgcacag attttaactt
     2341 gttcttcaat tctggaatca gtttcccttc aactacgccc gcagcaatag aataggaaat
     2401 gattaaacca aatggaccgg cgacgacatc ggctgcggca ccggcatacg cttccttcct
     2461 gattttatct acctgtgact ggaaatagct gcttttttct gaaaaatcat tggttaactg
     2521 gctatctaac gccagcagtt tcccggaagc gttgttgaaa ctttgtgagc ttaccagcag
     2581 ggatttttgc gcttcattca gcttcgtgat gccgtcatcc agtaccttaa tgaggggcgg
     2641 atgctttctt ctcattgtac tcatcaaata gcaaaatata cgctgcgagc aattgcgtcg
     2701 caacaccaca ccattcatac actgtttggg ttgcttcaaa atacttatcc tggctatcca
     2761 taagtaaggt tttaatatcg ccgactaaaa cggaggctgc ctgtgaatac tcctgtttaa
     2821 agcgacttaa ctcttttatg gtttcatcaa aggtctgcca ggggatgacc tgatcgagat
     2881 atttattata aagatctaat gctccatctg cggtttcgat tgcgttttta actacttcta
     2941 ccgttttatc tgcaacgatt tcagtcataa tcattcgcct ctttaaatat ataaattgta
     3001 atgaaactcc tgttttacaa ctattaataa attttacttc atctaattca tagttagccg
     3061 ggcgggatgc gtcaatgtct ttatttctat taatatgata aatatcaaac aatgtttaat
     3121 gtcattatgg cgaatgcttc tattctattt ttttgccggg tgatattttt catttctgct
     3181 ggatgagcgt cgtcgccaga aggccacgtg agcacaggat aagagaacga aaaatcagca
     3241 gtctatgcag cgacaaatat tgatagcctg aatcagtatt gatctgctgg caagaacaga
     3301 ccactgtata taaaaacagt ataacttcag gcagattatt atgttgttta tcaagcctgc
     3361 ggatctccgc gaaattgtga cttttccgct atttagcgat cttgttcagt gtggctttcc
     3421 ttcaccggca gcagattacg ttgaacagcg catcgatctg aatcaactgt tgatccagca
     3481 tcccagcgcg acttacttcg tcaaagcaag tggtgattct atgattgatg gtggaattag
     3541 tgacggtgat ttactgattg tcgatagtgc tattaccgcc agccatggtg atattgtcat
     3601 cgctgctgtt gacggcgagt ttacggtgaa aaaattgcaa ctacgcccga cggtacagct
     3661 tattcccatg aacagtgcgt actcgcccat taccatcagt agcgaagata cgctggatgt
     3721 ctttggtgtg gtgatccacg tcgttaaggc gatgcgctga tgtttgccct ctgtgatgta
     3781 aacgcgtttt atgccagctg tgagacggtg tttcgccctg atttatgggg taaaccggtg
     3841 gttgtgctat cgaataatga cggttgcgtt atcgcccgaa acgctgaggc aaaggcgctg
     3901 ggcgttaaaa tgggcgatcc ctggttcaaa caaaaagatc tgtttcgtcg ctgtggtgtg
     3961 gtttgcttta gcagcaatta tgagctttac gcagacatga gcaatcgggt gatgtcgacg
     4021 ctggaagaac tatcgccccg cgtcgagatt tacagtattg atgaagcatt ctgcgatctg
     4081 acaggtgtgc gtaattgtcg cgatctgact gattttggca gagaaattcg cgcaacggtg
     4141 ctacaacgta cccatcttac tgttggtgtg gggatcgccc agaccaaaac gttggctaag
     4201 cttgccaatc atgcggcaaa aaaatggcag cggcagacgg gtggggtggt ggatttatca
     4261 aatctggaac gccagcgtaa attaatgtct gctctccccg tggatgacgt ctgggggatt
     4321 ggacggcgga tcagcaaaaa actggacgcg atggggatca aaaccgttct cgatttggcg
     4381 gatacagata tccggtttat ccgtaaacat tttaatgtca tgctcgaaag aacggtgcgt
     4441 gaactgcgcg gcgaaccctg tttgcaactg gaagagtttg caccgacgaa gcaggaaatt
     4501 atctgttccc gctcgtttgg tgaacgcatc acggattata cgtcgatgcg gcaggccatt
     4561 tgtagttacg ctgcccgggc ggcggaaaaa cttcgcagtg agcatcaata ttgtcggttt
     4621 atctccacat ttattaagac gtcaccattt gcgctcaatg aaccttatta cggcaatagc
     4681 gcgtcggtaa aactgctgac gcccactcag gacagcaggg atatcattaa cgctgctacg
     4741 cgatctctgg atgccatctg gcaagcgggc catcgctacc aaaaagcggg cgtgatgctg
     4801 ggggatttct tcagtcaggg agtcgcgcag ctcaatttat tcgatgacaa cgcaccgcgc
     4861 cccgggagtg agcaattgat ggcggtaatg gatactctga atgctaaaga gggcagagga
     4921 acactctatt ttgccgggca ggggatccaa caacaatggc agatgaagcg agctatgctt
     4981 tcaccacgtt atacaacgcg aagttctgat ttactgcggg tcaaataaat atagcggcag
     5041 gaaaaaagcg ctcccgcagg agcgccgaat ggattagcga ccgaacagat cacgtttttt
     5101 cgctttaaac ggctgggaaa tcaccaccag caccgcgaca atcaggtaag cgataaaaat
     5161 accgagcagc cactgcggca tttccatacc taaaaaatcc cactgacgct cggcgcaatc
     5221 gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt ccgggaaacg
     5281 aaccataaaa tcacaggtgg caaatggcga aggatagagc tgaagcatgg tgtgctcgta
     5341 agttaactgc acaccgcgga acgcactata caaccagata accatcgcta cataacgcag
     5401 cggagttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga ataacgcgca
     5461 gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga accacagcgc
     5521 cgtcagttcc agtgccagag cagtaaacgc catcaacagc cacgcgcccc ggccttgtga
     5581 acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga gtgcgcagtt
     5641 taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca attggacaat
     5701 tatcgggcga aaaggcaaac cgggcattta tatgcccggt aagttgttat caaagcgttg
     5761 ctatccagcc catttgcata aaccattcgg ttacaggggc aagcgtaaac tcgacgcaga
     5821 gcaagccgac gagtgtcagt acgagggtgt aaggcagggc catccacacc atgcggccat
     5881 aagagaggcg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac gcagcctgac
     5941 cgttcggcgt agcgacagag ggcagattgg taccggtatt aatggcgacc gccagcagct
     6001 cgtattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg ttgatataaa
     6061 tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa atatagaaca
     6121 gcgacagctg agcatgttca gatgcctgca acacaaactg aataatcggc gaaaacagtt
     6181 gttggtcgat aatcaccgcg actaccgaga aaaacaccgt caacagtgcg gtgaatggca
     6241 gagattcggt gaaggcttta ccgatagcat gttcatcggt gacaccggtc aatgatgttg
     6301 ccagaataat gactgacaaa ccaatcaagc caacttccgc cagatgcaac gccagcgcag
     6361 tcaccagcca gacgccaata atcgcctgga caatcagacg gattttatcc tgacgggtac
     6421 gctggtggcg gctttgatcg tcaaactgtt gcaacacttc gcggactttc tccggcagcg
     6481 tttcaccgta gccaaaccaa cgcagcttct ctaccagcag gcaggttaac aggccacaaa
     6541 tcagaaccgg aacggtcacc ggcgacatgc gcaggaagaa atcgccaaaa tgccagccag
     6601 ccgctttagc gatgatcagg ttctgtggtt cgcccaccat ggtcattacg ccgcctaatg
     6661 cggtgccgac accggcatgc atcatcaggc tacgcagaaa gccacggaac tgttccagaa
     6721 ccacgttgta atgcttgtcg atatgactat cgtcttgcag gtcggtgtct tcggtacggg
     6781 aagaggctac gcgatgataa ataccataaa aaccgactgc aacgctgatc accaccgcca
     6841 cgacggttaa ggcatcgagg aacgcggaga ggaacgctgc cgccacgcaa aaagagagcg
     6901 acagcagcat tttggagcga atgcttaaca gcaaacgggt aaatatgaac agcaacagct
     6961 gtttcataaa atagataccc gccaccataa acatcaataa cagcaagact tcaagatttg
     7021 ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatacc gcttcgatag
     7081 ccaacagacc accggggagt agcgggtagc atttcagggc catcgccaga gtgaaaataa
     7141 attccgctac cagcaaccag cccgcgacga aagggctgat gaggaaaatt aacgggttta
     7201 cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc aaaaaattgc
     7261 gccatagcgc gcggccccag gagatctcca tgatggtttc ccttacctta caaataatca
     7321 atgatgtttt tatgtttaaa cgcaaagctt aacggtcagg caggagtgag gcaagtcttg
     7381 atagtcaagg ggaaagagat gcggaaaatg aagccttgat ccctttttct tctttttgtc
     7441 tgctatcagc gtagttagcc ctctggtatg atgagtccaa ctttgttttg ctgtgttatg
     7501 gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg tttcgcggaa gagtacatta
     7561 ttgaaagtat ctggaataac cgcttccctc ccgggactat tttgcccgca gaacgtgaac
     7621 tttcagaatt aattggcgta acgcgtacta cgttacgtga agtgttacag cgtctggcac
     7681 gagatggctg gttgaccatt caacatggca agccgacgaa ggtgaataat ttctgggaaa
     7741 cttccggttt aaatatcctt gaaacactgg cgcgactgga tcacgaaagt gtgccgcagc
     7801 ttattgataa tttgctgtcg gtgcgtacca atatttccac tatttttatt cgcaccgcgt
     7861 ttcgtcagca tcccgataaa gcgcaggaag tgctggctac cgctaatgaa gtggccgatc
     7921 acgccgatgc ctttgccgag ctggattaca acatattccg cggcctggcg tttgcttccg
     7981 gcaacccgat ttacggtctg atccttaacg ggatgaaagg gctgtatacg cgcattggtc
     8041 gtcactattt cgccaatccg gaagcgcgca gtctggcgct gggcttctac cacaaactgt
     8101 cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga aacagtgcgt cgctatgggc
     8161 atgagagtgg cgagatttgg caccggatgc agaaaaatct gccgggtgat ttagccattc
     8221 aggggcgata atcccttccg tttaaagagc aaccccctca aacgaggggt tttttgttgt
     8281 ttttacagat ttcccattct tggcgggcaa cgttccagca actcgatgct gccgtcttcg
     8341 ttttgctgtt cgagcatcac atcaaatccc cacaggcgat gcacatgctt caggacttct
     8401 ttgcgccccc gatccagcgg tgcgcgatta tgtggaatat aacgcagcgt cagcgaacgg
     8461 tcgccgcgca aatccacgtt ccagatctga atattcggct ccagattact taagttatat
     8521 tgcgacgata accggttacg gatctcccga taaccttctt cattatgaat agcggaaatc
     8581 tccagataat tatgccgatc atcatccagc acggtgaaga agcggaaatc acgcatcact
     8641 ttcggtgaca gaaactggct gataaaactc tcatctttga aatcacgcat cgcgaaatgc
     8701 agcgtttcca gccagtcgga accggcgata tccgggaacc agtatttgtc ttcttccgtt
     8761 ggcgactgac aaatccgttt aatatcctgg aacatggcga acccgagggc atacgggttg
     8821 atgccgctgt accacgggct gttatagggg ggctggaaga ccacattggt gtggctgtgc
     8881 aaaaactcca gcataaaacg ttccgttact ttcccttcat catacagatg gttaaggatg
     8941 gtatagtgcc agaaggtcgc acagccttcg ttcatcacct gagtctgttt ttgcggataa
     9001 aaatactggc tcaccttacg cacaatacgc aggatttcac gctgccatga ttccagcagt
     9061 ggcgcatttt tctccataaa atagagcagg ttttcttgtg gttcggacgg atagcggcgc
     9121 gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag catattgacc
     9181 tgactttgta gatactcttc gcgacttttc tgccgggctt tctcttcttg cagcgagatt
     9241 ttttgcgggc gtttgtaccg gtccacgccg tagttcatca gcgcgtggca cgagtccaga
     9301 agccgttcta cttcatcaac gccataacgt tcttcgcact cggtaatata tttacgggca
     9361 aaaatcagat aatcgacaat cgaactggcg tcggtccaac tgcggaataa gcaattgttt
     9421 ttaaagaaag agttatgtcc atagcaggcg tgtgccatca ccagcgcctg catagtaatg
     9481 gtgttctctt ccatcaagta agcgatacac gggttagagt taatgacgat ttcataggcc
     9541 agcccttgct gaccgtgctt atacagccgt tcagtctcga taaacttttt accgaatgac
     9601 cagtgcggat agttaattgg catgccgacg ctggaatagg catccatcat ctgttctgag
     9661 gttatcactt caatctggtg cgggtaggta tccagccggt agagtttcgc cacccggtct
     9721 atctctgcca gataaacatc cagcaggtcg aacgtccagt cgggtccatc gctcaaacgt
     9781 gtggtgtcct tattcataga atcgatcgtc gccatacgcg cacctcattg ttgtcggcgc
     9841 tctctgtgtg gagcacctca tttcaagcat agaacacctg ttaaaaaccg cgtcgccgga
     9901 gaattttttt ctttgcgatt tcttattatc agagtgccac taatccgctt ctgaacggaa
     9961 ttttatgctg gataaaaagg gcgttcagca ggagatacta aagacgccat attgccgcag
    10021 agtcagggag atgtgagtca gctcaccata aaaaagccgt atgttgaata atattttcaa
    10081 ctgagttatc aagatgtaat tagattatta ttcttttact gtatctaccg ttatcggagt
    10141 ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc gttgccagcg cctggtactt
    10201 aaatcaggca ggacatgagg tcaccgtcat tgatcgggag ccgggggccg ccctggaaac
    10261 cagtgccgcg aatgccgggc aaatctcccc cggatatgct gcaccgtggg cagcaccagg
    10321 tgtgccttta aaagcgatta aatggatgtt ccagcgccat gcgccgctgg cggttcgtct
    10381 cgacggtacg cagtttcagt tgaaatggat gtggcaaatg ttacgtaact gcgacaccag
    10441 ccactacatg gaaaacaaag ggcggatggt gcgtctggcg gaatacagcc gtgattgcct
    10501 gaaagcattg cgcgccgaaa ccaatattca gtatgaaggg cgtcagggtg ggacgctgca
    10561 actgttccgt accgaacaac agtatgaaaa tgcgacccgc gatatcgccg tgctggaaga
    10621 tgccggcgta ccgtatcagc tgctggaatc cagccgcctg gcggaagtgg atcccgcgct
    10681 ggcagaagta gcgcacaaac tgacgggcgg cctgcagtta cccaatgatg aaaccggaga
    10741 ctgccagctg tttacccaga atctggcgcg gatggcagag caggcggggg ttaaattccg
    10801 ctttaatacg cccgttgacc aactgctttg cgacggcgag caaatctacg gcgtgaagtt
    10861 tggcgatgaa gtgattaagg ccgatgcgta tgtgatggcg tttggttctt actcgacggc
    10921 gatgctcaaa ggcattgttg atattccggt gtatccgctg aaaggctact cgctgactat
    10981 tccaattgcg caggaagatg gtgcgccagt atccaccatt cttgatgaaa cctacaaaat
    11041 cgccattacc cgtttcgata accgcattcg tgttggcgga atggcggaaa ttgttggttt
    11101 taacaccgag ctgttgcaac cgcgtcgtga aacgctggaa atggtggttc gcgatctcta
    11161 tccacgcggc ggtcatgtcg agcaggcgac tttctggacg ggtttgcgcc cgatgacgcc
    11221 agacggtacg ccggttgtcg ggagtacacg ctttaaaaat ctgtggctga ataccggtca
    11281 cggcacgctc ggctggacga tggcttgcgg ttccggtcag ttgttaagcg atctgctctc
    11341 tggtcgcacg ccagcgatcc catatgagga tctaagcgta gcgcgctaca gccgtggatt
    11401 tacgccatca cgtccgggcc atttacatgg cgcacacagc taaggaaacg agatgacccg
    11461 tccgatacag gccagcctcg atctgcaggc attaaaacag aatctgtcca ttgtccgcca
    11521 ggccgcgccg cacgcgcgcg tctggtcggt ggtaaaagcg aacgcttacg ggcatggtat
    11581 tgagcgtatc tggagcgcgc tcggggccac cgatggcttt gcattactta acctggaaga
    11641 ggcaataacg ttacgtgagc gcggctggaa ggggccgatc ctgatgctgg aaggattttt
    11701 ccatgctcag gatctggaga tttatgacca gcaccgcctg accacctgcg tacacagcaa
    11761 ctggcagctc aaagcactgc aaaatgcgcg gctaaaagca ccgttggata tttatcttaa
    11821 agtgaacagt gggatgaatc ggttgggctt ccagtccgat cgcgtgctta ccgtctggca
    11881 gcagttgcgg gcaatggcga atgttggcga aatgaccctg atgtcgcatt ttgctgaagc
    11941 ggaacatcct gatggaattt ccggcgcgat ggcgcgtatt gagcaggcgg cggaggggct
    12001 ggagtgtcgg cgttcgttgt ccaattcggc ggcgactctg tggcacccgg aagcgcattt
    12061 tgactgggtt cggcctggca ttattttgta tggcgcttcg ccgtccggtc agtggcgtga
    12121 tatcgccaat accggattac gtccggtgat gacgctaagc agtgagatta ttggtgtcca
    12181 gacgctaaaa gcgggcgagc gtgtgggcta cggcggtcgc tatactgcgc gcgatgaaca
    12241 gcgaatcggc attgtcgccg caggatacgc cgacggttat ccgcgccatg cgcctaccgg
    12301 tgctcctgtt ttagtggacg gcgtgcgcac catgacggtg gggaccgtct cgatggatat
    12361 gctggcggtt gatttaacgc cttgcccgca ggcggggatt ggtacgccgg ttgagctgtg
    12421 gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc ggaacggtgg gctatgagtt
    12481 gatgtgcgca ctggcgttac gcgtcccggt tgtgacagtg taacttgttg taagccggat
    12541 cggaggcagc gtcttctggt tgcgaaaaaa tcatccatcc ggctggtcag caactgtagt
    12601 tgttaatgtg acagagccat tgcccatgat agtgtccatt aaaagaatgg acactatttc
    12661 cccggaacct gaactcgccg cacaggcgtt ctacataaaa cgcttacgct tcattgttga
    12721 ctcggagtcg acttcagata aatcacgctt cacccttgat gcagattcgg gttcatgttc
    12781 agattgttga ttaacaggct gcgagtcctg ttgtctttcc agactcaaca ggcccaactg
    12841 ttgatccagg agctccaggt cctcctgtac cttcacgcgc tccaggagtt ccgggtcatc
    12901 caggaactcc agatccataa ggtcctccag ctcctcgagt ccttccaggt caacctagcc
    12961 ttccttgtgc aaaaagtcca aaaggtcctt caggccctgc tgttttttca ggccctcgac
    13021 ggctttgagc agcgtcggta aattgtcttt gtcccacggg ttaccattac agtcaaacac
    13081 cttcaggctc tggggctgcg gcacctgtac cgttttaaac ttgttttctc cacagtagta
    13141 aattattagt ttgggcggca gccgtggtga agagatcaaa tcgttactag aacagtcaaa
    13201 tactttcagg cgagtcaagt tctccagcat ctcatcaggt attgagttaa tattgttgcc
    13261 ggcacaggat agtttataca ggtactgggg catcgacggc ggtaaaaatt gcaagaagtt
    13321 ttcagaacag ttaatcactc tcaggttagt cgacagctca ggcagccaga taatactgtt
    13381 ttcatagcac ttcagtacca tcagttttga gggctgcacc ttcggtaaca agatcaagac
    13441 gttatctgta cagtcaagca ccagcagcca ctttggcatc accttcggta acgcggtcaa
    13501 cacgttttta ctgcagtaaa attcaatcag atgtggaggc aaattctcag ggagtgaagt
    13561 caatcccagg ttgctaagat tgagcatact ccgtcttgta atgagacatt ccttcattct
    13621 gcgcaatgct tctgcccggt tttcaatccg gcttgctggc aggttttctt ccaggatatt
    13681 ttccttgatt tcttccagac tttctttcag gtcttcttgg ggaatttctg actgtttgtt
    13741 ttgctggttt tcttccagga tttcgtacag gtttttttgc aggttttctt gcaaaccatc
    13801 ctgctgtata gcccattcgc accatttggc gaccatcatg aactcgttat gttgctgatt
    13861 cagacgagtc cccaacgtga tgatggtatt gttgtccacc tgtatgttca ctttgcccgc
    13921 gatactgagg tcaatgatct gccagggggc atcaccccag ctgaattttt gcgggctgag
    13981 atacttcatg ttctgcaaaa tttgattctt gagaagagcc gccctggcgg gcgtcgtacg
    14041 cctgggttaa cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc tttaaagcta
    14101 gccataatgc tacgtttcct tgaaataaat atcctctggc agcgccggag acgtgaatgt
    14161 ggtcaccgca aaggagccac aggacgtcat ttgcgctgat atcactactc atcgttgact
    14221 tttttcctgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg gcctaggtag
    14281 ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt tattaagcaa
    14341 aggaataact ataagaaatc gctcatgcat ccggtgctac aggcggatga aacgcctatt
    14401 gtgtgggcta tgtcagtgcg gcaggttgcg tgggggaata tgttcaggcg atgtcaggca
    14461 ggggcccctg atggttgacc ggtaatgcgg ggctggtttt tattacctgt tacagtcgga
    14521 aaaagccctt acgctgactt tcttgagccg gtgttttgag ttcattagcc agagcctgca
    14581 atggtttgtc atccataatt taaccttctt ctgatgttgg gatgagcata tcaaaatcag
    14641 gaaatgacaa gtttattaca ggctcaggct gcgttcagta aagcatcgca tccggcgata
    14701 gatgctgcat taaatttaag attcagcttc ctcttcagcc acccgaacgc caatcttcaa
    14761 cacttcatta tcttctttct cggccaccgt ccagatcatc ccggcaaact ctacctggtc
    14821 accgacaacc ggtgccgcgc ctagcaactg ctggacaatt tcacccagcg tttgctgttt
    14881 atcacgatat tctcgcccgt cttccaggcc atatatcagc gcaacatcag catatttggc
    14941 gctggcttcg agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg gtggcgactg
    15001 gctgaacagt ttaccgagcg ccgggagatc gcgttcccga ccaattacac acaatacatc
    15061 gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac tggttatcac gaaacagggc
    15121 tgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt gccgcaccta cacaccattt
    15181 atcagcactc aattgataaa caaactgctc ccacggattt tccggatgaa tatccaggcc
    15241 aacgcgtgac accggacgtc cgacgggcgg aaccaccact ttggcttttt tcgccgccca
    15301 cgagagtgat gttccctgca agagcagtga aaccagaacc acaaagaagg cgacattaaa
    15361 gaacagtcgc gcattctcca gccccgccat catcgggaac acggctagga tgattggcac
    15421 cgcgccgcgt aatcccaccc agctgataaa cacgcgctca cgcagattga aaccgcggaa
    15481 agggagcaat ccagcaaata ccgaaagggg acgggcgaag aatatcatcc atgccgacaa
    15541 aatgagcgcc ggaatggcaa taggcagcag atcgcttggg ttaaccaata gccccagcac
    15601 caggaacatg gcgatttgcg ccagccaggc gaggccgtcg aaattttgca ggatgccgta
    15661 gcggttgcga atcggccgat tacccagcag aaaaccgcac agataaacag ccagaatacc
    15721 gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata ccgccgctta atgccagcaa
    15781 tggatataat ccggcaggca gggcgatgcg attaatcatt tgcagcagta aataaccacc
    15841 gccaagaccg atgacaatcc cgaggccaaa ttgctgcaga atatcgacca cgaacatcca
    15901 actgacactg ctttcatgct gctggatcat tgcaatcagg gtaatcgtca gaaagaccgc
    15961 cattggatca ttactgccgg attcaatttc cagcgtcgag ccaacacgtt cgttaagccc
    16021 cttaccaccc agcagagaaa agaccgctgc agcatcggtg gagccgacga tagcgccgat
    16081 taataagcct tcaatcaaat caagattaaa cagccacgcc gccatcatgc cggttaaacc
    16141 agaggtgata agtacgccca gcgtcgccag cgacagcgcc ggtcctaacg ccacacgaaa
    16201 ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata atcgccagcg ccaggttact
    16261 taccatgtag gcgaaggggt aattatcaaa cgggataccg ccgacgccat cgactcccgc
    16321 cagcatgccg atcgccagaa aaataaccag aataggaatg ccaagacggg aagaaaatga
    16381 actaagtaat atactgctgg tgacaaggat ggatcctaag atgaaaaggc taattattgt
    16441 tgtggcatcc aatggtcggt tactcctgat tacgctgtct cttatataaa ccctaccata
    16501 ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg atttttttac attttaagaa
    16561 caggatgacc gctaatcgtc aactgagcgc cttcccgggt gttattcaga atggcatgtg
    16621 cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa atcgaggccg gtaatgagtg
    16681 gaatcgacag gcgggaacgc aaaaacgcgt acactgactc caggttgtaa cctgcgtcat
    16741 aatcattggg cgtgctgccg ctaaagctac cgagaataat cgccttctga cgtggcaaaa
    16801 ttcccgcatg atagagctgc aacagcatac gttcgacgcg gaaagggtgc acgttaatat
    16861 cttccagcac cagaataccg ttctcaattt ttggcatcca cggtgtacca atcagtgaaa
    16921 tcagcatcgc aagattgcct ccccacaacg tgccttcggt ctgacatgtc gggccttccc
    16981 cttgccattc aatggtgaag gtttcattgc gtaacgccag ccagaaatgg tgctcggtaa
    17041 aggcgttcag ttcatccgca ccaaagtttg ctaccagcat ggggccgcta aaggtgatga
    17101 cattgccttg cgccagaaga ccgcactgaa tggcggtaaa atcgctatgt ccgcaaatga
    17161 gtaacgggtc atgttgttgg cgagccacca gtgcctgcca gtcaatatct gccagtaaac
    17221 gactggcacc gtaaccgccg cgtacagcca gcacgatggt gttgggggta gtcagtctgg
    17281 caagggaatt gagatcttcc agacgctccg tttccgtacc cgcaaaacgc tcacaacgac
    17341 gggcaataac ctcgacgtta ttgacttgat gccccgcgtc ggtaaggcgc tggataccac
    17401 gcagcgcggc gtgctgttta atgctgtaac ccgatggggc aattaagtga aacagagaca
    17461 tggcaattcc ttgctgacaa cagaaacgaa atgtatatca tgccgcttag gtgtgccgtt
    17521 gtcacctcaa cggcgattcc aggctataag gatagaagaa gtgaaattga gatggtttgc
    17581 ctttttgatt gtgttattag cgggttgttc atcaaagcat gactatacga acccgccgtg
    17641 gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg ccaataagcc agaaagccgg
    17701 tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt atcgctatcg aatcgggtgg
    17761 taatccgaac gcggtgagta aatcgaatgc cattggtttg atgcagttaa aagcttcaac
    17821 ctccggacgt gatgtttatc gccgtatggg ctggagtggt gagccgacga ccagcgagct
    17881 gaaaaatcca gagcgtaata tttcaatggg ggcggcttac ctgaatattc tggaaaccgg
    17941 cccgctggca ggcattgaag atccgaaggt actgcaatat gcgctggtgg tgtcatacgc
    18001 taacggggca ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg cgatcagcaa
    18061 aatcaacgat ttggatgctg acgagttcct cgatcacgta gcgcgaaatc atcctgcgcc
    18121 gcaggctccg cgctatatct acaaacttga gcaggcactg gacgcgatgt aaatcagtcg
    18181 cgcactttgt ccgctttttc ccgggcttct cgctcgagag agaaaataat ccgctgtaat
    18241 tgccgctcca ccgtcgggct gacgttaaga aaacggaagc tcagacgggg agtggtgatg
    18301 gtttcattct tgccatcaat cactttgcgc tcgctggtgg agattaactg ggcgtcaaag
    18361 tgaaaaacac cccattgccc catgttgact tcaatttgag cgaagcgcat accttcttgt
    18421 aattcggcag gctttgctgt ttccagtaat gcgcccatgc cgcctaacga caaatcatac
    18481 aagcggaaac gtaacgtact gttatccgcc agtttggtct ggcaaaaata aggcggatgg
    18541 agtggggcgg agatgcggaa atatcggcgt cgttgtacaa accataaggt gggaggcggt
    18601 acggtaataa atgccggaag ctgcaagtat tcactctgct gtagttgttc aacagtaaac
    18661 tcgactttcg caccctgagt ttcggcggta atggtaatgt gctgtgcctt tagcacggcg
    18721 atgttgtctt cggcttgact gccgaaatcc agcaccagtt tatccggggt tattgccagt
    18781 attttgctga tcagctgccc gccattccaa ctgagacgca aaggaattgc ggctttgtgc
    18841 aaatcgcgta acacgcccag gacggctaac ggattttgtt tcaggaactg ctcatggtaa
    18901 tgactcacgc ggaaaaactc ctgactgaca aactgtcttt ggtttatcgg tcatagttaa
    18961 caaaacttaa tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc agccgacaaa
    19021 cgcccagcat cgatcccgcc cttgcctata cttagagcgt tgacgtaagc atttcttgcg
    19081 accgattcat cgaaacgagg gcctgaacat gggaattatt gcctggatta tttttggcct
    19141 gatagccggc attatcggca agctaatcat gccggggcgt gatggtggtg gatttttcct
    19201 gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc tggctggcga ccatgtttgg
    19261 cattgacggc tccatcagcg gttttaattt gcacagcttc ctggtggcgg tggtgggcgc
    19321 tatcctggtg ctgggcgtat tccgcctcct gcgaagagaa taagattttc ataaggcgga
    19381 tagcgataca gatgccgcta tccgctttca catcagaacg tatactcgac acctgcccag
    19441 aaattgcgac cttcttcaat aaacccttca ctgtaggcat attgggtatc aaacaggtta
    19501 ttgaccgacg cattgacgct gaagccatga cctaaggtgt aatcggctcg aatgtgggtc
    19561 accgcaaaac cggcggcttt ttgtgaaccg tcactgttgc tgtagctgga ggaacgcgcc
    19621 tcttccgaca gcgttacgct taacggctcc catggtttga gtgtcatcca tgcggtcatt
    19681 gtctgcgttg gaagatcggt tatcttgccg atgtctttgc gtttggcgtc agcgtggatc
    19741 agagcgtagc tcaatcctac atccagtata ttgctgattt tccccttaat accggcatcc
    19801 agaccgctgt aatccaccgt gccgctgttc tgattttgaa tggtatcggc attgatattg
    19861 tgcgagagga tggcatcact cacccggtta tagtaaacgc tgacctcaaa tccccagtcg
    19921 cgcgtgaagg caccattcca ggttaaatcc accccgcgag cgcgttccgg cttgagctgc
    19981 gggttaacaa tcgctatctg gttatacgca ggtttggatg tggtatagcg ttctttcagc
    20041 gtcggaaagc gtgtgcggtc atagtacgaa agcgccagag tgtcttcatt ggcaaagtgg
    20101 tatttcccca tcacctgcca gttaaaagct gactgattgt tgtcgtcata atgggtgatg
    20161 ctgccatctt tctcgtgttt tttcgcttct acgctatcgc gccagtcata gctgattccc
    20221 gccacgacat cgacattatc agcagcagcc cattgatatt cactggcgag cgaccaggta
    20281 cgatcttcat agcgatcgta agcggcgtgc ggcgcacctt tttcccggtg tacgtcatct
    20341 ttccagttaa cggcaaacga cagcagatcg ttttcacgca catcggctgc cagttgtaat
    20401 ccggcaccgt cgctgtaatc ggaataatgg ctgtagctgc cttttttatt tttcaaatca
    20461 gccagcgagt tgtacatcat cagcgtattt tcaaaggtgt cgcgatacag ccgacttttc
    20521 agggtaaaac ggtcgtttag ttgggtcgct ccctgataat aaaaactttc tttgtcatac
    20581 tctggccact gccagtagcg tgatttttga ccacaatttc cgctgtatgg cgggttatct
    20641 ttttcaccat cctgcttaat gtaagtcagc gtgtattcat cgttttcgcg cggtgtaaaa
    20701 ccgagcttca caatgccgcg tttatcatca gccgatgaat taatcatctt gccgtgtttg
    20761 cctgcaatat cattatttac accatgcggc aggccgagaa aatcctgctt tagctggcta
    20821 ccgctgactt gcaaataccc aagatcgctg ctggcggcaa atgaagcatg catatcgtag
    20881 gcattgtcct gactacggct ccatccctgg cgatatccca gacttgcttc ttcagggtgg
    20941 tgatattaat ggctccgccc atctgattag gcccatgaag cagcgacgaa taccctttgg
    21001 aaacttcgac tgcccccaga ttgttggtca gaatccgcgc cagatcgagg ttgccgtcat
    21061 aggggacata aatgggcaca ccgtcgaaat agaccggcac ctgccgacta tcaaagccac
    21121 gaacttttac ctgttcttcg ttgcggctgc ctgacttttg cagcaccacg ccggggacga
    21181 cacttaatgc ctgagcgaca ttctgtttat ccaaggcctt catggtgggt tgatcgagaa
    21241 cggtggtcgt cgtcgatgat accggactgg accatacggt cagcgtgtcg ctctcctgag
    21301 ccgctacggc ctgctgggta aaggcaagtg ataatgctgt gcagagataa cgttttttga
    21361 gtctcataga ataaattccg tttaacaaag aattaaatag aggctgtggg ttgcagactg
    21421 actttaatgt cagctggtgc cgcgtaataa ggcgcggagg taataaaaag gcgaatgccg
    21481 cagtcgaggt aatttttgag tgtcgtcaga ttaattccgc cggtcagagc cagcgtgcag
    21541 tgaggagcca gcgacggcgc tatttgcgca atttcagttg cctgttgcgg actaaatttg
    21601 tcgagctgaa gtacgtctgg ttgtgcgcgt aacgcggcga tagcctcttt cggcgtgtcg
    21661 gcttcaacaa caattttctt ctcgggtgcg tggcgacgta actgagcgat tgcgcctgac
    21721 cagccctgat tgtcatgaag aaaatggcgg tggttggcaa acaataatat ggtttccgca
    21781 catccggcac gatgaattag tcctccggca gccagaattg cctgcgaagc cagtaagcgg
    21841 gtgcccggaa ttgcttttcg ggtgcaggcg atattgccgt caggataacg ttcacgaagc
    21901 aatgccagca tttgagcgag ataatcagaa acaccgcaac tccactccag cacattctgg
    21961 actgccttcc atccctgatg aagtgctgcg gcattaccct gcgcacggat tagccgttga
    22021 cccgcgttcg cttgtgaacc gtcgctgacc gcgtcatcaa tagttaatcc cagcgtagtt
    22081 aacatcttac acgcgacaga aacgccgctg acgcagccgc cctgacggtg aaaaaacgct
    22141 atatagccag gctggtgtcc aatatttaat gctcgcgtgg tcaggtcacc tccctggata
    22201 tcttccagca gtaacgcatc gatttgtgcc tgagaaagga aaatcatcac agtgcctcct
    22261 gcggtacgct atcccaccag atgagcgccc agtcgcggct ggcaggggcg agggcacgag
    22321 catcttgttg ttgataccag tgatataacc gctcacgctc atcgtcgtta agcgcgccaa
    22381 gactccagct gacgttctgc tcaaaacgtt cccaggtgct gttatcctgt tggcaatttt
    22441 gtccacggat aaaatcaacg tgggcataaa ttcccatctg atacaggacg ttgagcgcaa
    22501 agatatagtt gggtaattcg atcacttccc gaccagccgc ccgctgaatg gctggagaga
    22561 cgaacgaggt gctgaccaga tgagttgtat agacgcgaag ccgtgcctga ttattcagtt
    22621 tgctcattgc atggcgcata tcagccacca gagtggagcg ggaagccacg gcaatatcgc
    22681 agcgtggcag gtcactccag tcctcttccc aggcgcgctg gatccagtgg acattgtcag
    22741 ccttcagtgc ggcggcgcgg cgggcggcaa cgttcagcat cccctgactg taatcaacgc
    22801 cataaaccgt agtgagtttg tcagccagcg ccagcgatac ggtgcctggt ccgcagccca
    22861 tatcgaataa ggtctgagcg ccttgcaggt caattttagc gatcagttgt tgcaggtagc
    22921 tgtccgttgg cgaggcgcaa ttttccgcca ttttctccgc ccgttgatcc cagtgatcgg
    22981 gcgttttttc tgtgcggtga gccagtttga gttgctgcag ataaaggtct gcaaaatcaa
    23041 tatcatcaat gagcatgatt gttccttagc ggctaattgc agagaggtga tgatgaattt
    23101 ggtcggcaga aacccgatac agggccgcca gtttgttcgt tgttaactgt tcggtcggta
    23161 agccttgtgt tactcgtcca tctggctcta cttggataat gctgtcggca atggcgttgg
    23221 catggagcgt atggtgagtc gacattaaca ttgtcatgcc gttatttttg agctgcgcca
    23281 gcgtgtccag aagttggatc tgatgaccga aatcgaggct ggaggcgggc tcatccagta
    23341 acaacaagcg cggttgttgg acgagtgcac gggcgattaa agccagctga cgttcaccgc
    23401 cgctaagcgt attccagcgt cgtgtcgcca gatgcaaaat gcccagcttt tccagttgct
    23461 ccgtggcttt caggcgttct tgcttaccgg gaacggaaaa agcgcctatg gtgggggcca
    23521 ggcccatcaa caccatatcc agcacactga aagcaaaaat accgtcgtgc gcctgcggca
    23581 cccaggcaat agcctgtgcg cgctggcgat ggctgagtgt ctgcacagag aggctatcca
    23641 gtaggatctc gccttttaat aacggtataa cgccaagaat ggagcgcatg agtgtggttt
    23701 tgccgcaacc attcgcgcca agcagacaac aaattgttcc ttgcgacagg gtgaaggaca
    23761 cgtcgcgtag cactggatgg cgggagtaac ccagagacgc atgacgaacg gcaagcaaag
    23821 tcactggcga cctccgcgta acaacaaaag caggaaaaac ggtgcgccaa caaaagcggt
    23881 cagaatgcca agtgggattt ctgttgttcc aatgctgcgc gccagcgtat cggtaagcag
    23941 gagcagaata gcgccagtac acattgccat aggcaacagc tgctgatggt tatgaccggt
    24001 caacaagcgg gcaatgtgcg gaacgaccag accgatccaa ccaattatgc ccgctattgc
    24061 taccgtgctg gcggtgatta gtgtggcgca gacaatcaat ccaaagcgca gacgcgtcac
    24121 gttcagtccc agcgaacggg cttcgtcgtc ggaaagcgtg agtaaattca tgcgccagcg
    24181 gagaaaaaac aggggcaagg agcctatgag aatgatcggt gcagcatagc agagatcgcg
    24241 taaggtcacg gtggacagtc cccccagcaa ccagaaggta attgacggta actgggtgta
    24301 aggatcggcc agcgttttaa ttagcgatat ccccgctccg cacaacgttc ctaacgcaat
    24361 gcctaccagc accagggtta agatcggatc gtggcgggtc acccgacgcg taatcaacca
    24421 gacgcccgcg accaccatca gcccgccgca aaatgcgtac agctggatgt aaacaatggg
    24481 taatccccac aggattgccg cgcaggcacc aagtccggca ccggcggaga cgcctaaaat
    24541 atccggtgaa actaagggat tacgcaacat tccctgatag gtggttccgg ctcctgccag
    24601 cgctgcgccc agtaacagcg cagcaagaat acgcggcacg cggatttgcc agaagacaat
    24661 ttgttcctgc acagactcat tttcttgacg aagaattagc gccagcagtt gctgcatatc
    24721 gagatgataa gccccactta cggcggcaaa agtgatactg atagctgcca gcagcaaaat
    24781 acatccgttt acgattctca ttagctcgcc accaattttt gatagtcggc atcactgagt
    24841 gaacaatgcc agaacagttg ggcgtaatgc tgcatgtcac ttttaaactg gcgattgatg
    24901 gcgggatcca gccaggcgtg aagtctgcgt aatcccagaa gacggttgat tcccggcggg
    24961 gcatcgagcc agccaaaggg caagccactt aaaaataaga tgcgttgttc cgcaacggct
    25021 ttcacgccct gccagagagg atcacgacga ataaaatctg cagtaacggc ctcctgaacc
    25081 agaataatat ccggctgcca gcgcaggaga ttttccatgg aaacctgtgt caggccgtgg
    25141 cgatcggcta tttgcgcgac gttgtgcaaa cctaataatt ccgccgcctc ggtatgcaac
    25201 gaaccctgta agcccgtttc cagccctcgg ggaccgcgcg cagcataaaa gcggaggtta
    25261 gcggcgggtg aggtggcgaa tgcctgagct tcaccaacga agtggctggc gagattggct
    25321 tgttctgcgg cgcggtgctc ttcgcctaac gttttgccaa gcgttgttaa ctgttctgct
    25381 gattgcgcca gttttccgtt aagcaataac caggggattt gtgtctgttc gctaacctgc
    25441 cgtgcctggg agatcaaggt ttcatccgta ttgccgcaat caacgaccaa atcgggatgt
    25501 aacgccataa gcccttccag cgatagtgtg ctggcgcgcc cggcgagtcg tcccagcctg
    25561 ggtaactggc gaatgtgctc tggcaatgga attaatgcct gacgggcaaa atcaaaagag
    25621 gaaaaaccga ccattttttc tggcgcgaca gccagcaaga gcaaatcggc gggcgcgcct
    25681 gcgctgacga tgcgtgtaat gttttgggct tcgggaagag attgcgaagc aaaacgatta
    25741 actgtttgtg aaaaggacgg tagagactgc aggagcaggg tagatgcaag tgcctgcgca
    25801 aacgtccgtc tggttatggg catggtgaac atcctgaaa
//
LOCUS       DAJTYM010000056        25824 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTYM010000056 DAJTYM010000000
VERSION     DAJTYM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO575_002339"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO575_002339"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6665280.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO575_002340"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO575_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6665281.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO575_002341"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO575_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6665282.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO575_002342"
     CDS             3807..3995
                     /locus_tag="OO575_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6665283.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO575_002343"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO575_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6665284.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO575_002344"
     CDS             4756..5115
                     /locus_tag="OO575_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6665285.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO575_002345"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO575_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO575_002346"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO575_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6665286.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO575_002347"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO575_002347"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6665287.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO575_002348"
     CDS             10913..11254
                     /locus_tag="OO575_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6665288.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO575_002349"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO575_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO575_002350"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO575_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6665289.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13680..14966
                     /gene="dctA"
                     /locus_tag="OO575_002351"
     CDS             13680..14966
                     /gene="dctA"
                     /locus_tag="OO575_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6665290.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15187..16683
                     /locus_tag="OO575_002352"
     CDS             15187..16683
                     /locus_tag="OO575_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6665291.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16779..17708)
                     /gene="kdgK"
                     /locus_tag="OO575_002353"
     CDS             complement(16779..17708)
                     /gene="kdgK"
                     /locus_tag="OO575_002353"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6665292.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17940..18707
                     /gene="pdeH"
                     /locus_tag="OO575_002354"
     CDS             17940..18707
                     /gene="pdeH"
                     /locus_tag="OO575_002354"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6665293.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18777..20837
                     /locus_tag="OO575_002355"
     CDS             18777..20837
                     /locus_tag="OO575_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6665294.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21019..22341)
                     /locus_tag="OO575_002356"
     CDS             complement(21019..22341)
                     /locus_tag="OO575_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6665295.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22752..23765)
                     /gene="yhjD"
                     /locus_tag="OO575_002357"
     CDS             complement(22752..23765)
                     /gene="yhjD"
                     /locus_tag="OO575_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6665296.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23814..24713)
                     /locus_tag="OO575_002358"
     CDS             complement(23814..24713)
                     /locus_tag="OO575_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6665297.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25233..>25824
                     /locus_tag="OO575_002359"
     CDS             25233..>25824
                     /locus_tag="OO575_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6665298.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLN"
BASE COUNT         6156 a         6486 c         7029 g         6153 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc ccccccccca taaccttaca agacctgtgg ttttactaaa ggacacccta
    13681 tgaaaacctc tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta
    13741 ttctccttgg ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct
    13801 tcgttaagct cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca
    13861 ttgcgggcat ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact
    13921 ttgaaattgt cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc
    13981 ctggtgccgg aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg
    14041 ccgatcaggc gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg
    14101 tcattggcgc atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg
    14161 gttttgcgct ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg atcgaaagtt
    14221 tctcgcaggt catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgc
    14281 tcggggcaat ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc
    14341 agctgattat ctgtttctac attacctgta tcctgtttgt ggtgctggta ttgggttcaa
    14401 tcgctaaagc gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc
    14461 tgattgtact ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg
    14521 agaaactcgg ctgccgtaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta
    14581 accttgatgg cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta
    14641 acagtcagat ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta
    14701 aaggggcggc aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg
    14761 tgggccattt gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag
    14821 aagctcgtgc gctaactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt
    14881 gggtgaaaga actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg
    14941 gcaaaacgca cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag
    15001 tgcgggtgtt tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg
    15061 tttatttcac ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta
    15121 acgtcattta gactgaccga caacgggcag tttttttatt accaggatag ttgatcaggg
    15181 gttcacatgc agggcataaa aattcgactt ttagcgggcg gtttgctgat gatggccact
    15241 gctggctatg tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt
    15301 tccaacggtt tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa
    15361 attcgcctgc tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt
    15421 cacgccatcc ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt
    15481 tcattgtggc agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat
    15541 gacaccacgc tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg
    15601 ctctcttatc tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac
    15661 gcgctgcaaa gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg
    15721 cgctatcgtc tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa
    15781 cccgttgaag cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg
    15841 acgctactgg tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg
    15901 tttggcgaac tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg
    15961 cgtgcggaag cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg
    16021 tgggatacgc cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg
    16081 gacctggccc gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtgacagc
    16141 aaagacatcg gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc
    16201 aacatcgaat caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg
    16261 gcgaaggttc gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag
    16321 aaactggagc tgcagaaact gtttgccgcc tatgcacggg ctgataccga tattctgatg
    16381 ggccagcgga tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag
    16441 aaactgcggc aggatttcct taacagcctg actgtggaga tgttaaatca ggatctgcgt
    16501 cagcagttgt cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt
    16561 aacatgaaag cgttgcaggc ggcctgggat caaatcatgg ccccatctac cgccgctgcg
    16621 accacctctg tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag
    16681 taatgcctga tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga
    16741 ggggtaacac tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc
    16801 gggataatcg cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc
    16861 gcgttttccg cactgccgcc cgtcaaacgt accgccagat aaccggcgct gaaagagtcg
    16921 ccagctgcgg tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatcaact
    16981 aacgcttcac cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct
    17041 ttcacgcccg cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc
    17101 gcgtcttcgt cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgctggtac
    17161 acctgctgtg tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc
    17221 acttttccgc ctttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc
    17281 gggcttaaga tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct
    17341 tcgcaaatcg ccgcagactg ctcgctcgcc agccagaatt tggcggcggc ttcgttacgc
    17401 cagtagtaga acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag gcccggaaga
    17461 cggttttcca tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc
    17521 atctgctggc taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca
    17581 ggatcgacct gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc
    17641 ttaacgtccg cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt
    17701 ttggacatag tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt
    17761 tgctggtcaa ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg
    17821 aaagattccg ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgccctta
    17881 cgcgccgata atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga
    17941 tgataaggca ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg
    18001 aacggcgttt ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat
    18061 gcgggcggtt aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc
    18121 aacgcctgcc gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg
    18181 tgaaagagca gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacggcctgc
    18241 tggcatcggt caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc
    18301 tgcgccagat tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc
    18361 cgaaagattc aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg
    18421 gtaccgggat ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg
    18481 cgcgagaact gtttgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc
    18541 ttttacatct aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg
    18601 aagagtggcg tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac
    18661 gcccggcacc gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt
    18721 ccgccgggct ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga
    18781 gcaaggcagg caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg
    18841 cgatcatttt gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag
    18901 tctctgcgga gttgaatcgt ccgttcgcta tccgtggcaa tctgggcgtg gtgtgggagc
    18961 ggcaaaaaca agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag
    19021 acatcattct tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag
    19081 tagaggcaac gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca
    19141 agctcgaaaa gcccgacgcg cgcctgattc gcctctctga aaagaacaat aactggacgt
    19201 ttaatcttgc caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc
    19261 ggctggataa tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag
    19321 cggatctgga aatttttgtt gatccgttag gcaagccgct gccattcagc gaagttactg
    19381 gatcgaaagg taaagcggat aaagaaaagg tgggcgatta catttttggc ctgaaggcgc
    19441 agggacgtta taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc
    19501 tgcgtggcga agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg
    19561 ttgcttttga tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta
    19621 aattttctgg cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata
    19681 ccccgccgtt tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg
    19741 tctttgatta tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg
    19801 tctacaccac cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc
    19861 ggctggcgga cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga
    19921 aacggtctga acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt
    19981 atgaccgctt cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc
    20041 ggcgcattga gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca
    20101 aaaatgctga cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcgg
    20161 cgaatattca tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc
    20221 gtcgactgaa actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg
    20281 aaatgaacgg tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca
    20341 acagtaacgg caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg
    20401 agattgttgg gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg
    20461 tgcgggtgaa ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga
    20521 tttttgcttt cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt
    20581 cggaacagct ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc
    20641 gttcgccgct gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac
    20701 cgctgattgc ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccggcggcgg
    20761 cgttactggc actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt
    20821 cgcagatgaa gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt
    20881 agatttttgg caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc
    20941 tacaaatcct tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca
    21001 tcaggctttt cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc
    21061 gattaacgtc aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt
    21121 agcctgcaac caggctgcga tatacggcgc aacggaagcc ccgagaatcg acgatacgtt
    21181 gtaagagaac gatgctccgg tgtaacgcac ttctgtcgga aacagctctg gtaacagcgc
    21241 acccatcggc ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac
    21301 cagaatcggg ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat
    21361 cagcgtggtg atgatcacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc
    21421 gactggcacc atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt
    21481 acgcggcagg ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt
    21541 catgatgtaa aacagcgtat aggttgccag cataatgaac gtacccagta cggttacgcg
    21601 aacatgtttg gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac
    21661 tttctcaaac accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag
    21721 caccgccgag aagataaatg gcacacgcca gccccagctc ataaactgct catcggtcag
    21781 tagccaggaa agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcctag
    21841 ctgtgggaag gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag
    21901 cgccgcgccg ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag
    21961 cagcagtggg gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt
    22021 cgaaatcccc atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc
    22081 aaaatgacca aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc
    22141 gagcgactgt agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac
    22201 aataacggcc gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag
    22261 agaggcgaca aggactttat tacgcgagtt gatcggcgtg tattcttgtt cgtggtcgag
    22321 tgttgtggct gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta
    22381 cgcacagcaa aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc
    22441 aaaaagggga taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt
    22501 ataaatagtg gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc
    22561 tggggttatg agttggagtg aaattgtttg ctcccaaata gatggctgaa gaggctctgg
    22621 ggaagagttt caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat
    22681 ccggcctacg gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca
    22741 tccggcctat tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg
    22801 gtggcaatcc acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc
    22861 agccccagca cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg
    22921 taggtcatca cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc
    22981 agggcttttt tgcgcggacg gtggcgcggc aaccgccaga agatccagaa gaaaagcaga
    23041 tagttggcga agatggaaat cgccaggcca atcaatcgcc acgtcggttt cagccactca
    23101 atgctgttca ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg
    23161 atcgaaagcg tcacaatcag cgcaatcaac aaaccaatca gtgaaataaa atcacgcaga
    23221 tattttaccc agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg
    23281 gcacgaatcg cttcacgcag gttacccatc cagttgatgc cggaataaag tgccaccgcc
    23341 aggccgacaa gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac
    23401 gtggcggcta gtgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc
    23461 aacatcggat gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg
    23521 atcatcgaca aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg
    23581 ttaaagcgtt ctgtcgcgcg aataaggtgc gcaataaccg gttggcgttg gactttttcg
    23641 gcagtggtgg tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc
    23701 aactgcttaa tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc
    23761 gtcatccgct cttttgtcct tctgtcgtgg tcattcttaa aaagtatagt cggtcagtcc
    23821 aggtgctctt tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc
    23881 tcccgacgct gcgggtaaac cagcgaaagg gagagcggct cggcacggta gcctggcaac
    23941 acttcaataa gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata
    24001 atccccagac cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg
    24061 ccgccagact taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt
    24121 acacccaggt gcggtgtata acgcactatt gcgtgtgaag tcagatcatc ggggctttgc
    24181 ggataaccaa agcgcgtcag atagtgcgga ctggcacagt tgaccatggt tagtttgccg
    24241 aggggacggg cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa
    24301 ccatcatgaa gaatatctac cggacggtta tggctactca gttccagctc aattcccgga
    24361 tgctgataga gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg
    24421 atgtcgatgc gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt
    24481 tcgctgagat tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg
    24541 agtttgaccc gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc
    24601 tgtatctggc gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa
    24661 ctttccagct ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg
    24721 accgattgtt gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct
    24781 aacatctaat gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga
    24841 aaagggcgga atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt
    24901 gattcccttt aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg
    24961 ccatttgtta ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc
    25021 accaatataa ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg
    25081 cgtttagctg taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg
    25141 ccaccgccaa atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag
    25201 tctttttaac gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt
    25261 caatatttat tcatggaata aaaatcagtt tacagcagcg tattccagga gtgagtattc
    25321 agggggtcag tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag
    25381 ttatgctcga tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg
    25441 tgcagtttcc tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac
    25501 aggaagttat ccattttaat gtgctggcca ttgtgcctcg cgattcaacc gtcgagacat
    25561 ttgcgttggc ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga
    25621 cgacgccgga aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt
    25681 taaccatgtt agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta
    25741 ccggaacggt aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca
    25801 cccaggcggc aatgatgtta aata
//
LOCUS       DAJTYM010000057        25389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTYM010000057 DAJTYM010000000
VERSION     DAJTYM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..132)
                     /locus_tag="OO575_002360"
     CDS             complement(<1..132)
                     /locus_tag="OO575_002360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6665299.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     "
     gene            complement(265..570)
                     /locus_tag="OO575_002361"
                     /pseudo
     CDS             complement(265..570)
                     /locus_tag="OO575_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            638..1253
                     /gene="tnpA"
                     /locus_tag="OO575_002362"
                     /pseudo
     CDS             638..1253
                     /gene="tnpA"
                     /locus_tag="OO575_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1581..2663
                     /locus_tag="OO575_002363"
     CDS             1581..2663
                     /locus_tag="OO575_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6665300.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3152..3950)
                     /locus_tag="OO575_002364"
                     /pseudo
     CDS             complement(3152..3950)
                     /locus_tag="OO575_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3960..4511)
                     /gene="ybcL"
                     /locus_tag="OO575_002365"
     CDS             complement(3960..4511)
                     /gene="ybcL"
                     /locus_tag="OO575_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6665301.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4680..5012)
                     /gene="emrE"
                     /locus_tag="OO575_002366"
     CDS             complement(4680..5012)
                     /gene="emrE"
                     /locus_tag="OO575_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6665302.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5357..5671)
                     /gene="fliE"
                     /locus_tag="OO575_002367"
     CDS             complement(5357..5671)
                     /gene="fliE"
                     /locus_tag="OO575_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6665303.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5885..7544
                     /gene="fliF"
                     /locus_tag="OO575_002368"
                     /pseudo
     CDS             5885..7544
                     /gene="fliF"
                     /locus_tag="OO575_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7537..8532
                     /gene="fliG"
                     /locus_tag="OO575_002369"
     CDS             7537..8532
                     /gene="fliG"
                     /locus_tag="OO575_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6665304.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEEHAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8525..9211
                     /gene="fliH"
                     /locus_tag="OO575_002370"
     CDS             8525..9211
                     /gene="fliH"
                     /locus_tag="OO575_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6665305.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9211..10584
                     /gene="fliI"
                     /locus_tag="OO575_002371"
     CDS             9211..10584
                     /gene="fliI"
                     /locus_tag="OO575_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6665306.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10606..10947
                     /gene="fliJ"
                     /locus_tag="OO575_002372"
                     /pseudo
     CDS             10606..10947
                     /gene="fliJ"
                     /locus_tag="OO575_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10944..12071
                     /gene="fliK"
                     /locus_tag="OO575_002373"
     CDS             10944..12071
                     /gene="fliK"
                     /locus_tag="OO575_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6665307.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12176..12640
                     /gene="fliL"
                     /locus_tag="OO575_002374"
     CDS             12176..12640
                     /gene="fliL"
                     /locus_tag="OO575_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6665308.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12645..13649
                     /gene="fliM"
                     /locus_tag="OO575_002375"
     CDS             12645..13649
                     /gene="fliM"
                     /locus_tag="OO575_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6665309.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13646..14059
                     /gene="fliN"
                     /locus_tag="OO575_002376"
     CDS             13646..14059
                     /gene="fliN"
                     /locus_tag="OO575_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6665310.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14062..14427
                     /gene="fliO"
                     /locus_tag="OO575_002377"
     CDS             14062..14427
                     /gene="fliO"
                     /locus_tag="OO575_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6665311.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14427..15192
                     /gene="fliP"
                     /locus_tag="OO575_002378"
                     /pseudo
     CDS             14427..15192
                     /gene="fliP"
                     /locus_tag="OO575_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            15202..15471
                     /gene="fliQ"
                     /locus_tag="OO575_002379"
     CDS             15202..15471
                     /gene="fliQ"
                     /locus_tag="OO575_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6665312.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15480..16265
                     /gene="fliR"
                     /locus_tag="OO575_002380"
     CDS             15480..16265
                     /gene="fliR"
                     /locus_tag="OO575_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6665313.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16550..17173
                     /gene="rcsA"
                     /locus_tag="OO575_002381"
     CDS             16550..17173
                     /gene="rcsA"
                     /locus_tag="OO575_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6665314.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17217..17405)
                     /gene="dsrB"
                     /locus_tag="OO575_002382"
     CDS             complement(17217..17405)
                     /gene="dsrB"
                     /locus_tag="OO575_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6665315.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17568..17795
                     /gene="yodD"
                     /locus_tag="OO575_002383"
     CDS             17568..17795
                     /gene="yodD"
                     /locus_tag="OO575_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6665316.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18093..18908
                     /locus_tag="OO575_002384"
                     /pseudo
     CDS             18093..18908
                     /locus_tag="OO575_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18905..20599)
                     /gene="yedQ"
                     /locus_tag="OO575_002385"
                     /pseudo
     CDS             complement(18905..20599)
                     /gene="yedQ"
                     /locus_tag="OO575_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20770..20952)
                     /locus_tag="OO575_002386"
     CDS             complement(20770..20952)
                     /locus_tag="OO575_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6665317.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21031..21948)
                     /locus_tag="OO575_002387"
     CDS             complement(21031..21948)
                     /locus_tag="OO575_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6665318.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22121..23041
                     /gene="yedA"
                     /locus_tag="OO575_002388"
     CDS             22121..23041
                     /gene="yedA"
                     /locus_tag="OO575_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6665319.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23030..23500)
                     /gene="vsr"
                     /locus_tag="OO575_002389"
     CDS             complement(23030..23500)
                     /gene="vsr"
                     /locus_tag="OO575_002389"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6665320.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23481..24899)
                     /gene="dcm"
                     /locus_tag="OO575_002390"
     CDS             complement(23481..24899)
                     /gene="dcm"
                     /locus_tag="OO575_002390"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6665321.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24966..25389)
                     /locus_tag="OO575_002391"
                     /pseudo
     CDS             complement(24966..25389)
                     /locus_tag="OO575_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
BASE COUNT         6401 a         6493 c         6263 g         6232 t
ORIGIN      
        1 ggcaagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
       61 tgatacttca ctttctgcct gaatactgat gtaccactta ccgcaggact ggctgacagt
      121 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agccaagttt
      181 tggcagaaaa atacggctgt tttcctgagc gagcttaacg ccctgcggat agcggaatgc
      241 atcattctgt caccgctttt tgaatcaggg gaacgccgag cgcttctgga aaaagttttt
      301 gcaggcctgc tcagggtctt tcagtgactg ttgcaatggc tgtgacggag catctttaat
      361 ccattgcgtt tcagtggcat ttttccactc aaccaatcag gaagccattt tcgcgtaagg
      421 gatgtctttg ttaccggcct tatgattctc attctgacgt gccagagtac gactgaaaac
      481 gaaacgacat gcgcctgcga agcgcctcat ttcacgcgcc tgttgaccac cgggtcttaa
      541 ccggaatttg aatacctgta gtcgtttcac agggcaaatt atactttggt ctgtgaaaaa
      601 aactgatatc cgacgcggaa ggcactgtgt tttcctgatg catgctcacc gggtctttgt
      661 taccaagtac cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt
      721 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      781 cgtccatctg ttaattaact atccccccaa actggcgata tccagtctgg taaacaaaca
      841 gcctcaaagg cgtatcgggt aggttactgc gacgggatcg accagatatt gctgccaggt
      901 attaccgcaa agatgttttg tggcgtcccg gctattttgc cagtagctgc ggaggtgcgc
      961 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag atgaaaaaac
     1021 gagccttata tccccgccct gaaggacggg cttttacggt gcactggata agcctggaga
     1081 ttatctacac ctgcatggac atcattgtac tatgccccgt acatgctcca tcacatcctc
     1141 aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt
     1201 acgcaaaaaa aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaaaccacc
     1261 tcaattacat taataaatct aaataaatat catgcaaata atttccatgg ttatttttag
     1321 gctatttaaa ttaaaaaaat aaattatgag tcaatcataa ataacaacct ttaatataat
     1381 ttgactacca aatttatatt cgttcacaaa atagtcatgc attgtgtgca atagaaacag
     1441 ttcagataaa aatagggatc tacttcacaa tttaaatgag aaactaaaac ttacatcttg
     1501 aaataatctc attgattgga tgaatatttg tagtgcagtg acatcatttt taaataatag
     1561 ttcaaataaa agggctcacg atgaaaaaat taacagtggc aatttctgct gtagctgcat
     1621 cagtactgat ggcgatgtct gctcaggcag cagagattta caataaagac agtaacaagc
     1681 tggatctgta cggaaaagtt aatgctaagc actacttctc ttctaacgat gcagatgatg
     1741 gcgacacaac ttatgttcgt ctgggcttca aaggcgaaac tcaaatcaac gatcaactga
     1801 ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg tgctgaatct caaggttctt
     1861 ccaaagataa aacccgtctt gccttcgctg gcctgaaatt cggtgactac ggctccatcg
     1921 attatggccg taactacggt gtagcatacg atatcggtgc atggaccgac gttctgccag
     1981 aattcggtgg cgatacctgg acccaaacag atgtgttcat gactggtcgc accacaggtg
     2041 ttgcaaccta tcgtaacaac gacttctttg gtctggttga tggtctgaac tttgctgctc
     2101 agtatcaggg taaaaatgac cgcactgacg taactgaagc taacggtgat ggtttcggtt
     2161 tctccactac ttatgagtat gaaggattcg gtgtaggtgc aacctatgct aaatctgacc
     2221 gcactaatga tcaggttatc tacggtaaca acagcctgaa tgcatctggt caaaatgctg
     2281 aagtatgggc agctggtctg aaatatgatg cgaacaacat ctatctggct accacctatt
     2341 ctgaaaccca gaacatgact gtttttggta ataaccatat tgccaacaaa gcacaaaact
     2401 tcgaagtagt tgcacaatat cagttcgact tcggtctgcg tccgtccgtt gcttacctgc
     2461 aatctaaagg aaaagacttg ggtgcgtggg gtgatcagga cctgattgaa tatattgatg
     2521 taggtgcaac ctattacttc aacaaaaata tgtccacttt tgttgattac aaaatcaacc
     2581 tgattgataa gagcgatttc acgaaagcat ctggcgttgc taccgatgat atcgttgctg
     2641 taggattggt ttaccagttc taatctgatt actcaaagat atgttgtggg aggctttgcc
     2701 tccccaacat ataagtggcg ctctcaagcc actttcctta gaagcactat cttgcttctg
     2761 actatataaa ttacccttta ttttctggga cgtttcaacg ccccattttt aataatactc
     2821 agtgaacaat taacgtatca attagattta ataacaacga tatccgtacc taacctgata
     2881 tctaatgtca taagcatccc tttaattatc ccctcaacct cttgtaaatt tcccactctt
     2941 gagctccaca tagcccaaac gccggatgca aaaaaaatca tacgtgcatc tctgaaattg
     3001 tgatggagga agtgagtatc aattatatgt gcttataaac gttcccgatt ggcacagaag
     3061 tcaccggatt gttcaggctc caataggttg agtatgtcgt atttattttc caaaataaaa
     3121 atcgatgctg ttgctcctta ataaaatact tttactctct ggacgctttt cttagctctc
     3181 ttggtgttat attaaaacga ttatggaacc tttcagtaaa acgagaagga cacttgtaac
     3241 cattttccct ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca ggacaacgca
     3301 ttatttaacc tcacgtcgtc caatatactt tgaaaactta ttccctcgct tgccagacgg
     3361 cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc tgtccatttt
     3421 gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg tttactatct
     3481 gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc cattgctaag
     3541 tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg ctcccaacaa
     3601 tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat ttgatcagtt
     3661 atcgcataaa atttttgaaa cttttcaatt agatcaatgg gataataaag catttctgca
     3721 agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca gggaaataat
     3781 attatctgat tattatcaac agttagacta tgcgtctccc aattgataac tttttttccc
     3841 tgacggatac gacataaagc tggcataaga ggcttaaccc tgtgaatctc atgatgttta
     3901 tgcatccgta tttcttcaat ctttaggtta gacttatctc ttgccagcat actcttaccc
     3961 tattttatct cataaactgg tgttatctca gcggttgcaa ttttattggc attaagcata
     4021 taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt ttttagagcc
     4081 catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc tccaccaaat
     4141 cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt tgtaccatca
     4201 cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc aacagtccag
     4261 tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc aaagcttttg
     4321 gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc ttcacaccca
     4381 aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc attactagtg
     4441 acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact tgaaacgata
     4501 agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga tgtgccttga
     4561 tctcattatg cataaaggcg caatgatcgc gcaaatgcaa tcataattat aaaggtgccc
     4621 cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag aattattttt
     4681 taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca aatcaacatc
     4741 atgcctatga tagctggcag gtccagtcgt tgaccaaaaa atccccagga aagcaaacta
     4801 atcagaacga taccgactcc tgaccagata gcataagcaa ttcctgtagg tatataagcc
     4861 agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc aacagagggc
     4921 cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac ctctgcaagt
     4981 attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt catactggag
     5041 agaattgtac tacagtttga acacaactca ccgttttcat cctggtgtat gtgtatccaa
     5101 ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag gaaccccagg
     5161 ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt tatcgcgttt
     5221 acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac cagaaattga
     5281 gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa caagcatagt
     5341 caacaggtta cacaacctac acctgcatgg acatcacttc ctgatacgcc gccaccagct
     5401 tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata tcggtcatca
     5461 catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc gtgcgggcga
     5521 ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca aaactaatgg
     5581 tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt aactggctga
     5641 taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt tctggtaaca
     5701 aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa actggtttat
     5761 tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg agtgtgctga
     5821 cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag ccacgaagtg
     5881 cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc gcctgcgtgc
     5941 gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca tggtcgcact
     6001 gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt ccgatcagga
     6061 tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct tcagcgaagc
     6121 cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgg cgcaacaagg
     6181 tttgcccaaa ggcggcgcgg tcggtttcga actgctcgat caggaaaagt ttggtatcag
     6241 ccagttcagc gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat
     6301 cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt
     6361 attcgtccgt gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg
     6421 cgcactcgat gaagggcaaa ttagcgctat tgtacatctg gtttccagcg cggttgctgg
     6481 tctgccgcct ggaaacgtca cgctggtgga tcagggcggg catctgttaa cccagtccaa
     6541 taccagcggg cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg
     6601 tattcagcag cgtattgaag cgatcctgtc acctattgtt ggtaacggta atattcacgc
     6661 ctaggtcacg gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc
     6721 taacggtgat gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag
     6781 cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa
     6841 taacgcgcca atcagcacgc ctccgacaaa tcaaaataac cgccagcagc aggcgagcac
     6901 caccagcaat agtgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga
     6961 tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt
     7021 agtggtgaat tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat
     7081 gaagcaaatt gaagctctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgattc
     7141 gctcaatgtt gttaactcgc cgttcaatag cagtgacgaa agcggcggag cactgccgtt
     7201 ttggcaacag caagtgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggttgc
     7261 tggtactgct ggtggcgtgg ttgctgtggc ggaaagcggt acgcccgcag ctaacacgtc
     7321 gcgccgaggc ggtgaagact gtgcagcaac aggcgcaggc ccgcgaggaa gtggaagatg
     7381 cggtggaagt ccgtctgagc aaagacgaac aactccaaca acggcgcgct aaccaacgac
     7441 tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg
     7501 tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac aggcaccgat
     7561 aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt gttcaagcac
     7621 ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac gcagatctcc
     7681 aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca gtttgctgca
     7741 ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa
     7801 catgccgcca gcctgctgga agatattctc gaaacccgcg ataccgccag cggtattgaa
     7861 acgctcaact ttatggagcc gcagagcgcc gccgatctga ttcgcgatga gcatccgcaa
     7921 attatcgcca ccattctggt gcatctgagg cgcgcccagg ccgccgatat tctggcgttg
     7981 ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag
     8041 ccagccgcgc tggccgaact gaccgaagta ctgaatggct tgctcgacgg tcagaatctc
     8101 aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct gatgaaaacg
     8161 cagcaggaag aagccgttat taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa
     8221 atcatcgacg agatgttcct gttcgagaat ctggtggatg tcgatgatcg cagcattcag
     8281 cgtctgttgc aggaagtgga ttccgaatcg ctgctgatcg cgctgaaagg ggccgagcag
     8341 ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac
     8401 gatctcgcca agcgtggtcc ggtgcgtctg tcgcaggtcg aaaacgaaca gaaagcgatt
     8461 ctgctgattg tgcgccgtct tgccgaaact ggcgagatgg tgattggcag cggcgaggat
     8521 acctatgtct gataatctgc cgtggaaaac ctggacgccg gacgatctcg cgccaccacc
     8581 ggcagagttt gtgccaatgg tcgagtcgga agaaaccatt attgaagagg ccgaacccag
     8641 ccttgagcag caactggcgc aactgcaaat gcaggcccac gagcagggtt atcaggcggg
     8701 gattgccgaa ggtcgccagc aaggtcacga acagggctat caggaaggac tggcccaggg
     8761 gctggaacaa ggtctggcag aggcgaaggc tcaacaagcg ccaattcatg cccggatgca
     8821 gcaactggtc agcgaatttc aaactaccct tgatgcgctt gatagtgtga tagcttcgcg
     8881 cctgatgcag atggcgctgg aggcagcacg tcaggtgatc ggtcagacac caacggtgga
     8941 taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt tattcagcgg
     9001 gaaaccacag ctgcgcgtac acccggatga tctgcaacgt gtggatgata tgctcggcgc
     9061 aactttaagt ttgcatggct ggcgcttgcg gggcgatccc accctccacc ctggcggatg
     9121 taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct ggcaagaact
     9181 ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg ctggctaacc
     9241 acgctggata actttgaagc caaaatggcg cagttgcctg cggtacgtcg ctacgggcga
     9301 ttaacccgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc gctcggcgca
     9361 acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag cgaagtcgtt
     9421 ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtggaagg tgtcctgccc
     9481 ggcgcgcgtg tatatgccaa aaacatttcg gcagaagggc tgcaaagcgg caagcagttg
     9541 ccgctcggtc cggcgttatt aggtcgtgtt ctggacggta gcggtaaacc gctcgatggc
     9601 ctgcccttcc ccgatacgac ggaaaccggt gcgctgatta ccccgccatt taacccgttg
     9661 caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa tgccctgctt
     9721 accgtcggac gcgggcagcg tatggggctg tttgccgggt cgggcgttgg taaaagtgtg
     9781 ctgctgggga tgatggcacg ctacacccgc gccgatgtca ttgtcgtggg tttgattggt
     9841 gaacgtgggc gcgaagtaaa agattttatt gagaatatcc tcggtgccga aggacgtgca
     9901 cgctcagtgg tgattgccgc tccggcggat gtttctccgc tcctgcgaat gcaaggtgcc
     9961 gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt
    10021 atggactccc tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa
    10081 ccacctgcca ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc actggtcgag
    10141 cgtgccggta atggcattag cggcggcggc tcgattaccg cgttttatac cgtgctcacc
    10201 gaaggcgatg accagcagga ccccattgct gactccgcgc gggcaatcct cgacggtcac
    10261 attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga tattgaagcg
    10321 tcgatcagtc gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg agtgcgcacc
    10381 ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt cggcgcgtat
    10441 gccaaaggca gcgatccgat gctcgataaa gccatcgccc tgtggccgca gctggagggc
    10501 tatttgcaac aaggcatttt tgaacgcgcg gactgggaag cgtctctcca ggggctggag
    10561 cgtattttcc cgacagtgtc ataacccagg agataacggc agatggcgga agaacagctc
    10621 aaaatgttga tcgattatca gaatgaatat cgcaataacc tcaatagcga tatgagtgcc
    10681 gggatgacca gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc
    10741 attactcagc atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac
    10801 agctggcgag aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc
    10861 acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag
    10921 cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc cttgattacc gccgacgttg
    10981 acaccaccac attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcacgttgt
    11041 tgagcgaagc attagcaggc gagacaacaa ctgacaaagc ggccccccag ttgctggtag
    11101 caacagataa gcccacgaca aaaggcgagc cgctggtcag cgatattctt gccgatgcgc
    11161 aacaagcgga tttactgatc cctgtggatg aaacacttcc tgtcatcaac gacgaacaat
    11221 ccacatcaac accgttaacc accgctcaga cgatgacgtt ggctgcggtg gctgacaaaa
    11281 atacgacaaa agacgaaaaa gcggatgatc ttaatgaaga cgtcaccgca agtctgagcg
    11341 ccctttttgc gatgttgccg ggttttgaca atacgcccaa agtgactgat gcaccgtcaa
    11401 ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacta
    11461 cagcacagcc tgatgacgcc cccggcacac cagctcagcc attaacaccg ctggtagcag
    11521 aagcccagag taaagcggaa gtcatcagca cgccttcgcc ggtgaccgct gacgccagcc
    11581 cgctaatcac tccacaccag acacagccac tgcccaccgt cgccgcgcct gttttgagtg
    11641 caccgctggg ttctcacgaa tggcaacaat cattgagcca gcatatttcg ctgttcaccc
    11701 gccaggggca acaaagtgca gagttgcgtc tgcacccaca ggatttaggt gaagtgcaaa
    11761 tttccctcaa agtggatgat aaccaggcgc agatccagat gatttcaccg catcagcacg
    11821 tacgcgccgc cctggaagca gcgctgccgg tactgcgtac gcagctggcc gaaagtggca
    11881 ttcagttagg gcaaagcaac attagtggcg aaagctttag tggtcagcag caggccgctt
    11941 cccagcaaca gcaaagccaa cgcacagcaa accatgaacc tctggcgggg gaagacgacg
    12001 atacgcttcc ggttcccgtc tctttacaag ggcgtgtaac aggtaacagc ggcgttgata
    12061 ttttcgccta acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct
    12121 caagacgcag gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac
    12181 tgattacgcg ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat
    12241 taccctcgcg gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc
    12301 tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt
    12361 tacggtcaat ttgggcgatg cggatcgcgt gctttatatc ggcataaccc tgcgactgaa
    12421 agatgaagca acccgctcgc ggctgagtga gtatttgccg gaagtccgta gtcgcttgct
    12481 gttactgttt tcacgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct
    12541 gattgccgag attaaaacca cactttccac cccgcttgtt gccgggcaac cgaaacagga
    12601 tgtcaccgac gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc
    12661 tttctcaagc tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga
    12721 cagccagtat tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg
    12781 ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc
    12841 gtatggggct gttcaacctg ctgcgccgta gcccggacat taccgtcggg gccatccgca
    12901 ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aacctaatcc
    12961 acctgaaacc gctacgcggc acagggctgg tagtgttctc gccgagtctg gtgtttatcg
    13021 ccgtggataa cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt
    13081 ttacccatac tgaacagcgc gtcatcaacc gtatgttgaa gctggcgctg gaaggctata
    13141 gcgacgcctg gaaagcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg
    13201 tggaatttac caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg
    13261 tggagattgg caacctgacc ggcgaattta atatctgcct gccattcagc atgatcgagc
    13321 cgctacggga attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact
    13381 ggcgcgataa cctggtgcgc caggtgcagc attcacagct ggaactggtg gccaactttg
    13441 ccgatatctc gctacgcctg tcgcagattt taaaactgaa acccggcgac gtcctgccga
    13501 tagaaaaacc cgatcgcatc atcgcccatg ttgacggcgt cccggttctg accagtcagt
    13561 acggtactct caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa
    13621 attctctgaa cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa
    13681 cggcgcaatg gacgatctgt gggctgaagc gttgagcgag caaaaatcaa ccagcgaaaa
    13741 aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca
    13801 ggatatcgac ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg
    13861 gatgaccatc aaagagctgt tgcgtctgac gcaagggtca gtcgtggcgc tggacggtct
    13921 ggcgggcgaa ccgctggata ttctgatcaa cggttattta atcgcccagg gcgaagtggt
    13981 ggttgttgcc gataaatatg gcgtgcggat caccgatatc attaccccgt ccgagcggat
    14041 gcgccgcctg agccgttagt gatgaataac catgctactg tgcaatcttc cgcgccggtt
    14101 tctgctgagc cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc
    14161 gctgctgcct ggctggtaaa acggctggga tttgccccta aacgcactgg cgttaacggt
    14221 ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac gggtcgtggt ggtcgatgtg
    14281 gaagatgcac ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt
    14341 cccccttcag caccaacgga agagataccg cagaccgatt ttcagtcggt catgaaaaat
    14401 ttgcttaagc gtggcgggag atcctgatgc gtcgtttgtt ttctgtcgca ccggtccttc
    14461 tctggctgat tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctac
    14521 ctggcgggta tcaccagcca gccgctacct ggcggtggac aaagctggtc gctcccggtg
    14581 cagacgctgg tgttcatcac ctcgttgacg ttcattccag cgattttact gatgatgacc
    14641 agtttcaccc gcatcattat tgtttttggt ttattgcgta acgcgctggg aacgccctcc
    14701 gcgccaccta accaggtatt gctggggctg gcactgtttt tgaccttttt tattatgtcg
    14761 ccagtgatcg acaaaattta tgtcgatgcg taccagccat tcagcgaaga gaaaatctca
    14821 atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg agtttatgct gcgtcagacc
    14881 cgcgaggcag atttagggtt gtttgccaga ctggcgaata ccggcccgtt gcaggggcct
    14941 gaagctgtgc cgatgcgcat tttgctcccg gcctacgtga ccagcgagtt gaaaaccgca
    15001 tttcagatag gcttcacgat tttcatccct tttttgatta tcgacctggt gattgccagc
    15061 gtgttgatgg cattggggat gatgatggtt ccccccgcca ccattgctct gccctttaaa
    15121 ctgatgctgt ttgtactggt ggatggctgg caattgctgg tcggttcgct ggcgcagagc
    15181 ttttacagct agagaggcaa aatgacacct gaatcggtca tgatgatggg aactgaagcg
    15241 atgaaagtcg cgctggcact ggcagccccg ctgttgctgg tagcgctggt cacgggcctt
    15301 atcatcagta ttttgcaggc agccacgcag attaacgaaa tgacgctgtc gtttattccg
    15361 aaaatcatcg ccgtatttat cgccatcatt attgccggac cgtggatgct caatctgttg
    15421 ctggattacg tccgcacctt gttcactaac ctgccgtata tcatcgggta gcccgtacaa
    15481 tgatgcagga gacaagcgat caatggcttt cctggttaag cctgtacttc tggccgttac
    15541 tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag cgaacgcagc gtgccgaaac
    15601 gggtaaaact gggtctggca atgatgatca cgttcgccat tgccccatca ttacctgcca
    15661 acgatgttcc tgttttttcg ttctttgctc tgtggctggc cgtacagcag atcctgatcg
    15721 gcattgcgct tggttttacc atgcaatttg cctttgccgc tgtgcgaacc gctggcgaaa
    15781 ttatcggtct gcaaatgggg ctgtcatttg cgacatttgt cgatcctggc agccatctca
    15841 atatgcccgt tttagcgcgc atcatggata tgctggcatt actgctgttc ctgacattta
    15901 acggtcattt atggttgatt tcactgctgg tcgatacctt tcacaccctg ccgattggta
    15961 gcgaaccgtt gaacagcaat gcgtttctgg cacccaccaa agcagggagt ttgattttcc
    16021 ttaacgggct gatgctggcg ttaccgctca ttactctgtt gctgaccctg aatctggcat
    16081 taggtttact gaatcgtatg gccccgcaat tatccatttt tgttattgga tttccattaa
    16141 ctctgactgt cggcatctct ttaatggcgg cattaatgcc gttaattgcg cctttttgcg
    16201 aacatttatt cagtgaaatt tttaatttgc tggctgatat tattagtgaa ttaccattaa
    16261 tataattccg taacgtttat catgctaccc taaggattat ccgaaaaata atatctgcga
    16321 acatcttcca ggatactcct gtagcgaaat atttgtttta agctcactca catatcgcaa
    16381 catttacttt aagacaattc caggcaaatt atacaacact ttacgggata gtaagtccgc
    16441 ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt gttccgttta gtcatgatga
    16501 aatattcagg taaggggaat tatcgttacg cattgagtga gggtatgcca tgtcaacgat
    16561 tattatggat ttatgtagtt acacccgact aggtttaacc gggtatctgt tgagtagagg
    16621 ggttaaaaaa agagaaatca acgacattga aaccgttgat gaccttgcca tagcttgtga
    16681 ttcacagcgt ccttcagtgg tgtttattaa tgaggactgt ttcatccacg atgcttctaa
    16741 cagtcagcgc atcaagcaca tcattaatca acatcccaat acgttattta tcgtttttat
    16801 ggcaattgcc aatgttcatt ttgatgaata tctattggtc agaaaaaatt tattgatcag
    16861 ttctaaatcg attaaaccgg aatctctcga cgatatcctt ggcgatattc tgaaaaaaga
    16921 gacaacgata acctcgtttt taaatatgcc gacgttatca ttgagccgaa ccgaatcgag
    16981 tatgttgcga atgtggatgg caggtcaggg aaccattcaa atctccgacc aaatgaatat
    17041 caaagccaag accgtttcat cgcataaagg taatattaaa cgtaagatca aaacgcataa
    17101 taaacaggtt atctaccatg tcgtccgact gacggataat gtgactaatg gtatttttgt
    17161 caacatgcgc taacacattc tgactggtgg tttcccacca gtcaggctga ataagattac
    17221 tctgctttct caacaaagat accgtcctga tgccctgctt cattaaagaa ccagatgccg
    17281 agcgggtagt cttccagcgc aaccaggtac attgtgcctt cactaaactc ctcaactgcc
    17341 agtaccactc caggacgacg cggaccgcca tccgttttga ctgttacccg atcattcacc
    17401 ttcatcgttt tcctcctgtg gctttgtgcc agtgtagaac aatttcgttt ttctggcagc
    17461 gccaagcgcg cgcgagtgct gattttctcg acggtctata cttaagagat gccaggcgga
    17521 cttaacgact ggcggcaaca acagagtaac ggttgcgagg aaagatgatg aaaaccgcaa
    17581 aagtgtacag cgataccgca aaacgtgagg tcagcgtcga tgtcgatgcc ctgctggcgg
    17641 cgatcaatga aattagcgaa agcgaagttc atcgcagcca gaacgattct gaacacgtta
    17701 gcgtcgatgg acgtgaatat catacatggc gtgaattggc ggatgccttc gaactggata
    17761 ttcatgactt cagcgtctct gaagtgaatc gttgaatgca caataaaaaa atcccgaccc
    17821 tgagggtgtc gggatgaaac ttgcttaagc aagaagcact taaaaaattc gttacaccag
    17881 gaaatctgat gtgttcatca ccttatctgc aatttttttc gctgacaaga aaatattcgc
    17941 gctatgaatg attaacgcta cttatgaata gaaatgtgac gcgcgtcacc ccttggtggc
    18001 tctggtgtca acgggatcgc tcccgaaaaa atcatctttg attatttgtg ctgttttgag
    18061 aatcatcctg gtaaggggag tactgcgcga ccatgttttc aattcaacaa ccactactgg
    18121 tttttagcga tcttgatggc accctgctgg acagtcatag ttatgactgg caaccggcag
    18181 ccccctggct cagccgttta cacgaagcca atattcccgt cattctctgt agcagtaaaa
    18241 ctttagcgga aatgctgtac ttgcaaaaaa tgttggggct acaaggctta ccgctgattg
    18301 cagagaatgg cgcagtgatc cagcttgctg aacaatggca ggatatagac ggttttccac
    18361 gcatcatctc aggtattagc catggcgaaa tctgccaggt tttaaatacg ctacgcgaga
    18421 aagaacattt taaattcacg acttttgatg atgtcgacga tgcaaccatt gccgaatgga
    18481 cgggattaag ccgtagctag gcggcgctga cgcagctgca tgaggcgtcg gtaacgctaa
    18541 tctggcgcga cagtgacgag cgtatggcac aatttatcgc tcgtctgaac gaactgggct
    18601 tgcagtttat gcagggtgcg cgcttctggc acgtactgga tgcctctgcc ggaaaagatc
    18661 aggctgccaa ctggattatc gcgacctatc aacaattgtc aggcagacgc ccaaccacac
    18721 ttggcctggg cgatgggcca aacgatgcgc ctttactgga ggtaatggat tacgcggtga
    18781 ttgtgaaagg gctaaaccgt gaaggggtac atctgcatga tgaggatccg gcccgcgtct
    18841 ggcgaacgca gcgtgaagga ccggaaggct ggcgtgaagg gctggaccat tttttctccg
    18901 cccgttaagc gttatcgctc gcgaataccc gattgcgccc ggcctgtttt gcgaggtaaa
    18961 gccgacggtc ggccaatgac tggagttgtt caaaatcata atcaccggtt tcctcgctgc
    19021 tacttacccc cagcgaggca ctgatgcgta tcgttgtact cttggcgatc agcatctctt
    19081 tttcatttaa cttcaggcga atacgttctg cgacctccgc agcctgcgtc agattcgcgc
    19141 ctggcagaat cacacaaaac tcctcaccac cgacccgccc ggcaacgtcc tgcgcacgca
    19201 aggaactgct aattaatccg gcagcatgag aaagaacacg gtcgcccgcc tgatgaccaa
    19261 agcggtcatt aatcgcttta aaatggtcaa gatcgacctg aatgacagaa aaaggatgtt
    19321 ggtgcgtctg acacaattta gcgagcggac gggctttttc gaacagtgcg ccacggttat
    19381 ataaacgcgt taaggtgtcg tgccacgcct gccactgcaa cgagctttgc agaacataca
    19441 tgttgctgac catccggcga atcacatacc aggagaggag taacatggtg gtaaagagcg
    19501 cccacagcag ggttaatgca atgctgatac tgccgaaatc gccgcgcacg ccttcgctta
    19561 gcgtatggac gcgcgccagc acaccgtcga aatggtccag acgttcccag ctaacatagc
    19621 gactgtccat acgaatgccg ccccgcgtgt catgttccat tgcctgcgcc agcaaggcca
    19681 gttcacgagg atcaaaaata ttccctgttg gatggtcagg attggaagag gtcaaaaatc
    19741 tcagcttact gtcatagagc tgatactcac catcgaggtt tttatcgatg gcgtttctta
    19801 aaaattgctg catagtacgc acgggaatac tcatccccag cacgccatac cagtagttat
    19861 tactgtctac cggaacactg acggtaacct gcggttcagt attgctggcg tgttccggtt
    19921 gcgaagtgaa ccagcgtacc gcgcggtgac gattttctcg ttgcgaatgg ccgataaacc
    19981 agggttgggt gacatagccg taataacgcg ttggtacatt gcgcgtaaac aaggtcggct
    20041 gcgtcgaaac gtaaaatcca gcacgcgaga catacatcgc ctgttcaacc atcgacgagg
    20101 agttgtgcgc cagtcgcagc aagtaaccaa cttccagtgc agcggtaatt tcattgtcga
    20161 ggctttcatt ttcgcgagac aggaaattcc cctcgctgac taatgcatcc gatacgccat
    20221 tgactggcag ggtgcgccgt cggttgagtt cgatttgcca ggcgtgctcg tcgcgatgct
    20281 gttcgaactc ggttacggca ttacgcagag aggtgaaatc cagtggcgcc acgagagctt
    20341 cgcgcatgcc attacgcaaa aagatcagtt tgtcgacgtt atactgcaaa tgcttatcga
    20401 gcgcgttggc tacgttttcc agatgattac gctagctgga gatataggca tcttccagca
    20461 ccaccacttc acgccaggtg agcaaggtgg aaaaaagcaa taccacgata aagcagagat
    20521 taacgacatg accgggcccc aggcggcgtg cgagtttttt caaccagctc tggttttcca
    20581 tttttgtctc gtgctgcacc ctgacgactc ccaacctgct tttttatgat tctggcataa
    20641 cccggcaata gcgtaccgca aaacagcgat aattttataa tggtggcatg ataagacgcg
    20701 actatcattc attgcaggca ttgggacacc aggccggatg tgatccggcc tggtgttaat
    20761 gatacccttt caaacttctt ccgcagaacg actcccctcc cccggaacaa gatcggtttc
    20821 atgaaaagct tcccgcttga ccccgtaacc gtcataccaa cgacactcaa ccataccgct
    20881 ggagtatcca gtgacaatca tccgcgggcc gccctcttta accgtaactt cctcactaac
    20941 cataaagctc atactcgcct ccttttttcg agtgaaactt gttcacctta gttgaagatg
    21001 gcgaattttg cataatagcc agtgcgagta ttaatgtgcc tgaccgcgca ttttcgctac
    21061 tgctttcact cccagcacca cgatgccgcc gatgataaat ccaagaatca gatttaaaac
    21121 agtcggtaat atcatcgcca ccactgcact ttgctgcccg gcgatatgtt caatagcgtg
    21181 atgcagcggc gcaataccat gtaccacaat cccgccgccg acgaggaaca tcgccagcgt
    21241 gccgacaatc gataatgctt tcatcagcca aggcgcgata atcaataacc ctttacctaa
    21301 tgcctgcatc agcgcgctag atttttccgc cagccaatac cctaggtcat caatcttaac
    21361 gataacccct accagaccgt agacgcccac ggtcactacc agcgcgatgc ctgaaagcac
    21421 cagcacctga ttaagcaacg gcgcctctgc cacaatcccc agcgtgatgg cgacgatttc
    21481 cgcagacaaa ataaaatcgg tacgaatcgc ccctttaatt ttgtcctttt caaacttcag
    21541 cggatcctgc gccgccagct tctccagacg ctgctggctc tgcgctggat cttctttatg
    21601 tttacgcgcc tccagcatat gcagcacttt ctctacccct tcaaagcaga gaaacgcgcc
    21661 accaatcatc aacagtggcg taatcgccca cgggataaac gcactgatga tcagcgccag
    21721 cggcaccaga atcactttat tgatcagcga tcctttcgcc acgccccaga ccacgggaag
    21781 ttcccggttg gcccgcacgc ctgaaacttg ttgcgcattg agcgataaat catcccctaa
    21841 tacaccggcg gttttcttcg ccgccagttt gcccatcaca gagatatcgt ccagcagtgt
    21901 ggcgatatca tcgagcaacg tcagtaaact actcccggcc aaaagcattc cctctcattt
    21961 ttattgttga agggtgtaag tatgaagcaa aaacggcagc tctgatactt gcttcacagg
    22021 tcaacattta tttaacaatc aagtgcaatt atatctctcg ccagcagaat gattttcatg
    22081 tttactatga accacctttt tatttccgcc gtgagggatt atgcgtttcc gccagttgtt
    22141 accgcttttt ggcgcgctgt ttgcgttgta tatcatttgg ggctcaacct attttgtcat
    22201 tcggattggc gtgggaagct ggcctccgtt aatgatggcg ggcgttcgat tccaggcagc
    22261 cggtatttta ttgctggcat ttttgctact gcgcggacac aaacttcccc cgctacgtcc
    22321 gctgctcaat gccgcgctga ttggcctgtt attgctggct gtcggtaatg gcatggtgac
    22381 ggttgccgaa catcaaaatg ttccttccgg catcgccgcc gtagtgattg caaccgtgcc
    22441 cctctttacc ctgtgcttca gtcgcctgtt tggcattaaa acgcgcaaac tggaatgggt
    22501 gggtattgcc attgggcttg ccggaatcat catgctcaat agcggtggaa atttaagcgg
    22561 caatccgtgg ggcgcgattc tgattttaat cggctcgatt agctgggcgt ttggctcagt
    22621 ttatggctcg cgcattacct tacctgtagg gatgatggcg ggtgcgattg agatgctggc
    22681 ggcaggcgtg gtgttaatga tcgcatcgat gattgcgggt gaaaaactga cggcgctccc
    22741 ttccctgtca ggcttccttg cggtcggcta tctggcgctg tttggttcga ttatcgccat
    22801 taacgcttat atgtatttaa tccgtaatgt cagtccggct ctcgccacca gctacgccta
    22861 cgttaacccg gtggtcgcgg tcttgctggg tacgggactg ggtggcgaaa cgctgtcgaa
    22921 gattgaatgg ctggcgctcg gcgtaattgt tttcgcggtg gtactggtca cgttgggaaa
    22981 atatctcttc ccggccaaac ccgttgtcgc gccagttatt caggatgcat caagcgagta
    23041 aatgaatccc ctgcgtgtcg atctgcgcgc tggcaccttc gccgcagatc cactcttcca
    23101 gacgctcggt aagcgccgca tccgtcagct tctcgcgcct acgtaacgca cactcccaga
    23161 caatcagtac gcgccagccg agctcctgca agcgactgat atcgcggcga tcgcgttcaa
    23221 catttttacc tatcttctcc agccagaact cagttcgagt cgcaggcact ttaaacagat
    23281 agcagtgatg atgatgccag aagcagccgt gggtaaatat cacgcagcga tattcatcaa
    23341 cgacaaaatc cgggcgtccg ggcagactgg cgtcctgaac gcgaaacgca aggccctgcc
    23401 cggttaacaa actggcgagg cgcttctcaa tcgccgtatc acgcgtggca atcgcgcgca
    23461 tatttttgct gcgggtggcc ttatcgtgaa cgtcgaccat gttgtgcctc ttgctgacgc
    23521 aacgccaccg cctgtttgat ttttggctca agcagttttg ccaccgcggc aaagaccggc
    23581 acgaccaccg agttgccgaa ctggcgatag gcctgagtgt ccgaaaccgg aatgcggaat
    23641 ttcgcttctc ccggcgcttc aaaacccatt aagcgcgcgc attcacgagg cgttaaccga
    23701 cgtggacgat gttgctgatt cagcggatcg tcaaagtctt tctcacccgt ggccatatcc
    23761 caaccgcgat cgattaaaat ttccgcgcca tctttgtaat aacgagcaga cagcgtacgg
    23821 gtgacgcttt gcggattgtt cggataaacc attccataac cgaagccgtt accgcgcgcc
    23881 tgatgttttt tcgcatatcg atagaggtac ttccacagca ccggcgtcag gatatatttc
    23941 gcctcaacca tcgggtccag cagctgcgcc agcgtcactc gctgtgcagg gaaacattcg
    24001 ctgatatcac gcagagtaaa atcggctttc agattcagat cgcgacgaaa acccaccagc
    24061 acgatgcgtt cacggtgctg cggcaggaaa tgtttaccat cgatgatttt cggatcgtcc
    24121 ggcccgttat cttctgcatc ggcaacgtca tagcccagtt cgtccagcgt ctgcatgatg
    24181 atgcggaacg tttttccctt atcgtgactc ttcaggtttt tgacgttttc gagcacaaac
    24241 atcgccggac gacgcgcgtc gataatgcgc accacatcaa agaacagcgt gccctgggta
    24301 tcgcaggcaa aaccgtgcgc ccgcccgagc gagttctttt tcgatacgcc agccagcgaa
    24361 aatggctgac aagggaaacc ggccagtaaa acatcgtgtt caggaatgtg ttgacgaata
    24421 tgttccgccg ccgcctcatc actcacgcct tcttgatgac tgagggtgat gtcgcggata
    24481 tcttcattaa aatgatgcgt cgccgggtcg caataatggt tggctttata agtgcgtacc
    24541 gcatgtttgt tccattcgct ggtaaacacg cactgtccac caatcgattc aaaaccacga
    24601 cggatgccgc caattccggc aaatagatcg ataaagcgaa acgcataatg cggatgatgt
    24661 tccggtggtt tgggtaacaa cgtttgcaga tgggcaaact ctttctcact taaacggtgc
    24721 catgccgagt cattcgccag cgcacgtttt aaaattgccg cgctccagtg attctcaccc
    24781 acaccattaa gctgcgccac cagcatttta acatcataaa tttgcagcag tttctccagc
    24841 attgcctgtg cggcattccc ggtgctgtat gaatcggtta ctgatatatt ttcctgcata
    24901 gatttaaccg gccatctgag atggccgaca ggttaacata attactgccg tttagggcca
    24961 cgcgctcagc cagcgggtga aaacgcatct atcaccttgt gatcgatacc ttcattctcc
    25021 cccgccagtt cggcaccgag cttcgccatc aaacagcgcc aagctcaatg cccctgaaac
    25081 ggcaaatacg cgcgccaggc caatcgcccc cagggcttcc agccgatccg catcctggac
    25141 gattttagct tccgttgtta agggggcgat ttgcgcgctg aaactgtgag cggcaatggc
    25201 atgacaaacg gcctcaattt tctccgccgg aaattgcacg aactcttcac ggagcaggcg
    25261 acgcgtctct tctgccgcca ggattgaaga acgctgccgt tgcggatgat ttttcgccag
    25321 gctcacgata tcgtgaaaat aacaggcggt taaaatcacc agcatatcga catcgtcatc
    25381 tgccgccag
//
LOCUS       DAJTYM010000058        24922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTYM010000058 DAJTYM010000000
VERSION     DAJTYM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1284)
                     /gene="yeaG"
                     /locus_tag="OO575_002392"
     CDS             complement(<1..1284)
                     /gene="yeaG"
                     /locus_tag="OO575_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6665322.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKIL"
     gene            1720..2466
                     /gene="mipA"
                     /locus_tag="OO575_002393"
     CDS             1720..2466
                     /gene="mipA"
                     /locus_tag="OO575_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6665323.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2556..3317
                     /locus_tag="OO575_002394"
     CDS             2556..3317
                     /locus_tag="OO575_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6665324.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3368..4252)
                     /locus_tag="OO575_002395"
     CDS             complement(3368..4252)
                     /locus_tag="OO575_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6665325.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4336..5331)
                     /gene="gapA"
                     /locus_tag="OO575_002396"
     CDS             complement(4336..5331)
                     /gene="gapA"
                     /locus_tag="OO575_002396"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6665326.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5673..6086
                     /gene="msrB"
                     /locus_tag="OO575_002397"
     CDS             5673..6086
                     /gene="msrB"
                     /locus_tag="OO575_002397"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6665327.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6128..6400
                     /locus_tag="OO575_002398"
     CDS             6128..6400
                     /locus_tag="OO575_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6665328.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6770..7846
                     /locus_tag="OO575_002399"
     CDS             6770..7846
                     /locus_tag="OO575_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6665329.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7873..9252
                     /locus_tag="OO575_002400"
     CDS             7873..9252
                     /locus_tag="OO575_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6665330.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9269..10314
                     /locus_tag="OO575_002401"
                     /pseudo
     CDS             9269..10314
                     /locus_tag="OO575_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10335..11171
                     /locus_tag="OO575_002402"
     CDS             10335..11171
                     /locus_tag="OO575_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6665331.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11176..12123
                     /locus_tag="OO575_002403"
     CDS             11176..12123
                     /locus_tag="OO575_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6665332.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12133..13113
                     /gene="ydjG"
                     /locus_tag="OO575_002404"
     CDS             12133..13113
                     /gene="ydjG"
                     /locus_tag="OO575_002404"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6665333.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13250..14008
                     /locus_tag="OO575_002405"
     CDS             13250..14008
                     /locus_tag="OO575_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6665334.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14126..15484
                     /locus_tag="OO575_002406"
     CDS             14126..15484
                     /locus_tag="OO575_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6665335.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15577..16218)
                     /gene="pncA"
                     /locus_tag="OO575_002407"
     CDS             complement(15577..16218)
                     /gene="pncA"
                     /locus_tag="OO575_002407"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6665336.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16229..17245)
                     /gene="ansA"
                     /locus_tag="OO575_002408"
     CDS             complement(16229..17245)
                     /gene="ansA"
                     /locus_tag="OO575_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6665337.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17412..19268)
                     /gene="sppA"
                     /locus_tag="OO575_002409"
     CDS             complement(17412..19268)
                     /gene="sppA"
                     /locus_tag="OO575_002409"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6665338.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19429..19980
                     /locus_tag="OO575_002410"
     CDS             19429..19980
                     /locus_tag="OO575_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6665339.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20097..21140
                     /gene="selD"
                     /locus_tag="OO575_002411"
     CDS             20097..21140
                     /gene="selD"
                     /locus_tag="OO575_002411"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6665340.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21145..23106
                     /gene="topB"
                     /locus_tag="OO575_002412"
     CDS             21145..23106
                     /gene="topB"
                     /locus_tag="OO575_002412"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6665341.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23233..24273
                     /locus_tag="OO575_002413"
     CDS             23233..24273
                     /locus_tag="OO575_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6665342.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24390..>24922)
                     /locus_tag="OO575_002414"
     CDS             complement(24390..>24922)
                     /locus_tag="OO575_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR6665343.1"
                     /translation="SGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPV
                     DIALPCATQNELDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAAN
                     AGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANI
                     AGFVKVADAMLAQGVI"
BASE COUNT         6334 a         5762 c         6441 g         6385 t
ORIGIN      
        1 gaggatctta aacgcaaaac gcgtcgacag accgttcatc ccttcatcga caccggcgta
       61 gtcacgatat tcctgatacg acttggcttt cggatcggtg tctttcagac tttcgccatc
      121 ataaacccgc atctttgaat aaatgctgga gttttctggc tctttcaggc gcgaaagaat
      181 ggaaaaacgt gacagtgttt cgagcgtgcc aggggcgcat ggggcgtgag tcaattcact
      241 gtgattaagc aatttctcgt agattttgat ctcttcggaa atgcgcaagc aatacggcac
      301 cttcacgatg taaacacggt cgaggaaggc ttcgttgttt ttgttattac ggaaagtgac
      361 ccattcggac ttgttcgagt gggcgagaat aatcccgttg aacggcaggg cggagatacc
      421 ttccgtcccg ttgtagttac cttcctgagt ggcggttaac aagggatgca gcacttttat
      481 cggtgcttta aacatctcaa cgaattccat gatcccctga ttggcgcggc acagcgcacc
      541 ggaatagccg taggcgtccg ggtcattctg cgcgtagtgt tcgagtttac gaatatcgac
      601 tttcccaacc agcgcggaga tgtcctggtt gttctcatca ccgggttccg ttttggcgat
      661 agcaatttgt tgcagaattg acggccagac cttcactacc cggaacttag tgatatcgcc
      721 accaaattca tgcaggcgtt ttgccgccca tggcgacatg atggtgccga gataacggcg
      781 aggaatgcca tactcttttt ccagaatctg agcatcttcc tgcggattga aaagacagaa
      841 cggatgatcg ttgaccgggc tacgctcacc gttggcgctc aatacataaa tcggtacgag
      901 ctgcattaat gatttcagtc gctcagcaag cgatgattta cccccaccca caggccccag
      961 cagatacagg atttgtttct tctcttccag cccctgagcc gcgtgtttca gataagagac
     1021 aatctgttca atcgcgtctt ccatgccgta aaactcttca aatgccggat aacgtgcaat
     1081 gacccggtta gaaaagagtc gagaaagtct ggggtcctgg gctgtatcga ccatgacagg
     1141 ctcaccgata gccatcaata gccgctcagc cgcgttggca taagcactgc gatcttgccg
     1201 acaagtggta agaaactcct gcagtgtgaa ctcttcgtcc ttggcagctt catatcgctg
     1261 gcgatagtga tcgaatatat tcatggtatg ccgtcctttc gttttttagc acaggttaag
     1321 agccgttcat atgaatagta gaggctcccg gaagaggtaa actgaacgac gtctcttgct
     1381 aaaacacagc aactctcatg ccattttatc gacgatgaga acaacgtgcc cggtgataca
     1441 ccttcaaaat taagcgtaga tggcaaatgg aaaacttgcc tgcacggctt tcttaatttc
     1501 aatggcatat caacaactca tccataaatt ttgcataatt aatgtaaaga ccaggctcgc
     1561 cagtaacgct aaatccattt ggctgtaagc gcggtgtcat ccgcggcagg aaaattaaac
     1621 agttacttta aaaaatgaaa acgtaaaaag gttgggtttc gatgtattga cgggtaaact
     1681 ttgtcgcccg ctaaacattt gtttaaggaa tgattaattg tgaccaaact caaacttctg
     1741 gcacttggag tgcttatcgc aacgtctgca ggcgtagcgc acgctgaagg taaattttcc
     1801 ctgggcgcag gcgtaggtgt cgttgagcac ccatataaag attacgatac cgatgtttac
     1861 ccagtaccgg taatcaacta tgaaggcgat aacttctggt tccgtggctt aggtggtggt
     1921 tactacctgt ggaatgacgc aacggataaa ctttcaatta ccgcttactg gtcgccgctt
     1981 tacttcaaag cgaaagacag tggcgatcac caaatgcgtc acctggatga ccgtaagagc
     2041 accatgatgg ctggtctgtc ttatgctcac tttacccagt acggttacct gcgtaccacc
     2101 ctggctggcg ataccctgga taacagcaac ggcattgtct gggatatggc ctggttgtat
     2161 cgttacacca acggtggcct gaccgtgact ccgggtattg gtgtgcagtg gaacagcgaa
     2221 aaccagaacg aatactatta tggcgtatcg cgcaaagagt ccgctcgcag cggtctgcgt
     2281 ggctataacc cgaatgacag ctggagccct taccttgagc tgagcgccag ctacaacttc
     2341 ctcggcgact ggagtgttta cggtaccgcg cgctacaccc gtctgtctga tgaagttact
     2401 gacagcccga tggtggataa atcctggact ggcctgattt ctaccgggat cacctacaaa
     2461 ttctgataat gcatctttcc agggcatttt gtgcataaaa acagggcgtc acgccctgtt
     2521 ttgcattacg gtggtgcaat acctgggagg aaacaatgca acaaaaaatg attcaattta
     2581 gtggcgatgt ctcactgcca gccatagggc agggaacatg gtatatgggc gaagatgcca
     2641 gtcagcgcaa aacagaagtt gctgcactac gcgcaggcat tgaactcggt ttaaccctca
     2701 ttgataccgc cgaaatgtat gccgatggcg gtgccgaaaa ggtggttggg gaagcattaa
     2761 ccggtctgcg agagaaggtc tttctcgtct ctaaagtcta tccgtggaat gctggcgggc
     2821 aaaaagcgat aaatgcatgc gaagccagtt tacgccgtct caatactgac tatctcgatc
     2881 tctacttatt acactggtct ggcagtttcg cttttgaaga gactgttgct gcgatggaaa
     2941 aattgatcgc ccagggaaaa atccgtcgct ggggcgtttc taaccttggt tatgccgata
     3001 tgcaggaact ctggcagctg ccggggggaa atcagtgtgc cactaatcag gtgctttacc
     3061 atctcggttc acgagggatt gagtacgatc tactcccctg gtgccagcaa cagcagatgc
     3121 cggtgatggc ttacagtcca ttagcccagg ccgggcggtt gcgcaatgga ctgttaaaaa
     3181 acgcggtagt caacgaaatt gcacatgtcc aacaacatgc agctgcgctt gaggtcgaac
     3241 tttcgtcagc ggaattagct atgctggata aggcatatcc ggcaccaaaa ggaaaaactg
     3301 cgctggatat ggtgtgatgt tgatacgtgc cggatgcatc gcacccggca cacaacatta
     3361 acgcaaatta acgtttggca acgcgaatgg attgcgccag atgtgcaggt ttctctttgg
     3421 tgactttttg cgtttctgaa gcgtaagccg tttctacaca aacaaaggtc ttgtagccat
     3481 cgtccggcat gtcgcccatg ctaactgaaa gcgccggtcc cgggttccag ccgacaacgt
     3541 tcagatgatg ctggtggcct acggcgataa tacgattcag cgcttcatca ttaatcacgc
     3601 tgcaatcttg cggattcaga tacacgcgat cggtacggtc agggaaggtc tgaataccgt
     3661 cggtcagtac atcttctttc gcgtcattca ctttatcaat gaagcgatcg cccagcccac
     3721 tgacgcttac cttagcgata tcacccacgt taaagtaggt atgcagggca gaggtggttt
     3781 caaattcgcc atgtgattca agatcgattt cacaggtttt acccacgcgg aaatgcgcta
     3841 acagcgtaaa gtcgtgcggc cagaattttt tcgtctcttc gctttgtgtc aattcaaaag
     3901 tcagcgctac gccatcagca tcttcatgat gtgatttcag cgtccacggc aggttgcggg
     3961 caaaaccgtg cgcaggcaga ccttgttgtg ccgccggacc aaaccacggc cagcaaaccg
     4021 gtacgccacc gcgaatagcg acgccatttt tgaacggtgt gttgttgctc aaccacagaa
     4081 cttcttcttc acccgcaggt ttccacgaga gaaggtgtgc gccctgtaat gcaaaagagg
     4141 cttttacctg gggatgatcg accacaatga ggtccagttc atccagttta cgacgggaga
     4201 ggacagggga gatttgttcg atgaccggaa gggcaaaaat tttcttaatc atgacgcagt
     4261 cctttaactt cattttatca ggtaaaaaaa agagcgaccg aagtcgctct ttttagatca
     4321 cagtgtcatc tcaacttatt tggagatgtg agcgatcagg tccagaactt tgttggagta
     4381 accggtttcg ttgtcgtacc aggataccag tttcacgaag ttgtcgttca gagcgatacc
     4441 agctttagca tcgaacacgg aagtgcaaac ttcgccgttg aaatcggtag atactacgtc
     4501 atcttcggtg tagcccagaa cgcctttcat ttcgccttca gcagcagctt taacggcagc
     4561 tttgatctgc tcgtaagttg cagctttttc cagacgaacg gtcaggtcaa ctacggatac
     4621 gttcggggtc ggaacgcgga acgccatacc agtcagtttg ccattcagtt ctggcagtac
     4681 tttacctaca gctttagcag caccggtaga ggacgggatg atgttctggg aagcgccgcg
     4741 gccgccgcgc cagtctttgt gagacgggcc atcaacggtt ttctgagtag cggtagtagc
     4801 gtgaacggtg gtcatcagac cttcgatgat gccgaagtta tcgttgataa ctttagccag
     4861 cggagccagg cagttggtgg tgcaggaagc gttggaaacg atgtcctggc cagcatattt
     4921 gtcgaagtta gcgcctttaa cgaacatcgg agtgttgtct ttagacggac cagtcataac
     4981 cactttcttc gcaccagcgg tgatgtgttt acgagcagtt tcgtcagtca ggaacagacc
     5041 agttgcttca gcgacaacgt caacacccac ttcgtcccat ttcaggttag ccggatcacg
     5101 ttcagcggta acacggattt ttttaccgtt aacgatcaga tgaccgtctt tcacttcaac
     5161 ggtaccgtcg aaacggccgt gagtggagtc atatttcagc atgtatgcca tgtaatcagc
     5221 gtctaacagg tcgttgattg caacgatctc gatgtcagaa cgtttctgag cagcacggaa
     5281 aacaatgcga ccgatacggc caaaaccgtt gatacctact ttgatagtca tatattccac
     5341 cagctatttg ttagtgaata aaaggttgcc tgtaaaatta caaaaacctt acgcagcgtc
     5401 aagcggaatc gtgtcaatca ttgcgacaaa tcaatcctgt gcctaagcat tacgcgactg
     5461 actcgcctca cttttccttt gggcttgaga ccacatgggg tcggcgcccc gaattttaaa
     5521 gggcaattac gataaaaatg tgattttcat cacgatttag gtgcagccat ttcgtcgaga
     5581 tcaagtttag gacaaaattt tgccagttgg tgttaatgtt ttgttagaat cagtcacgta
     5641 atgtgagcac gattaaagtg agatgtgagt aaatggcgaa taaaccttcg gcagaagaac
     5701 tgaaaaaaaa tttgtccgag atgcagtttt acgtgacgca gaatcatggg acagaaccgc
     5761 catttacggg tcgtttactg cataacaagc gtgacggcgt atatcactgt ttgatctgcg
     5821 atgccccgct gtttcattcc gaaaccaagt atgattccgg ctgtggctgg cccagtttct
     5881 acgaaccggt gagtgaagaa tccattcgtt atatcaaaga cttgtcacat ggaatgcagc
     5941 gcatagaaat tcgttgcggt aactgtgatg cccatctggg gcatgtcttt cccgacgggc
     6001 cgcaaccaac aggcgaacgt tattgtgtta actctgcttc tttacgcttc actgatggcg
     6061 aaaacggcga agaaatcaac ggttgaaaaa acgattcagc aacttattcc acaggagcgg
     6121 aaaaattatg aatcttgatg acattatcaa cagcatgacg cctgaggtat accagcgttt
     6181 gtcgaccgcc gttgaactgg ggaaatggcc tgatggcgtt gcgttgaccg aggaacaaaa
     6241 agagaattgc ctgcaactgg tgatgctgtg gcaagcccgc cataataccg aagcacagca
     6301 tatgacaatt gacacaaatg gtcagatggt catgaagagc aaacagcagt taaaagaaga
     6361 ttttggtatc tcagcaaagc ctattgccat gtttaagtaa gtggttcatc ccctttttgc
     6421 cgcggctatg aaggggatag ccaacaatga ccccacgact cgtctcaatt atctcgaccc
     6481 gacctggcta agtcccagtc cgatctcttg cagtcgcgtt cacgcctgaa tgacttctcc
     6541 tgaacttaag tctcagacct atttggccgg taatccctct cgaatttgct ctttttgcgt
     6601 cccgccttcc accaacctgt aacagaccca gccattttct gcgtaattgt gatttgagtt
     6661 gtaaaatgca ggtcattatc tgtgttgtgc agatttaata ttgacctcaa aaatcatgtg
     6721 ggtaagtatg atggccatct aagcaaaaca aaacacagag gatgacgcaa tgaaagcact
     6781 ggctcggttt ggcaaggcct ttggcggcta caagatgatt catgtcccac aacccatttg
     6841 tggcccggaa gacgtagtga ctgaaattaa agccgcggca atctgcggcg cagacatgaa
     6901 gcactacaat gtcgatagcg gttctgatga gtttaactct atccgcggcc atgagttcgc
     6961 aggttgtatt gcgcaggttg gtgaaaaagt caaagactgg aaagtggggc aacgcgtcgt
     7021 gtcggataac agcggtcacg tttgcggcgt ttgtccggcc tgtgaacagg gtgattttct
     7081 gtgttgtaca gaaaaagtaa accttggtct ggataacaat acctggggcg gtggtttttc
     7141 caaatattgt ctggttcctg gtgaaattct caaaattcat cgtcatgcgt tgtgggaaat
     7201 ccctgatggt gttgattatg aggacgcagc cgtacttgac cctatctgta atgcctataa
     7261 atccatcgcg cagcaatcga aattccttcc tggtcaggat gttgtcgtca ttggcactgg
     7321 cccactcggg ctgttttccg tacaaatggc gcggattatg ggggcggtaa atatcgtcgt
     7381 cgttggtctg caagaagatg tggcggtccg cttcccggtt gcaaaagaac tgggggcgac
     7441 ggcagtagta aatggttcta ccgaagatgt ggtggcgcgc tgccagcaaa tttgtggcaa
     7501 agataatctg ggactggtga ttgaatgctc cggtgccaat atcgcactga aacaagccat
     7561 cgaaatgctc cgcccgaatg gggaagtggt acgcgttgga atgggcttca aacctcttga
     7621 tttctcgatt aatgacatta ccgcctggaa taaaagcatc attgggcata tggcctatga
     7681 ctccacctca tggcgtaacg ctatccggct attagccagc ggcgctatca aagtcaaacc
     7741 gatgatcacg catcgtatcg gcctgtcgca atggcgcgaa gggtttgatg cgatggtcga
     7801 taaaaccgca atcaaagtga tcatgactta cgactttgat gaataactac tctcctgaag
     7861 gaacgttatc gcatggaaca gataacaaaa ccgcattgtg gtgcacggct ggatcgctta
     7921 ccggattgcc gctggcattc gtcaatgttt gccatcgtcg cgtttggctt gcttgtctgc
     7981 tggagtaatg ccgttggtgg cttgatcctc gcgcagctga aagcgttggg ctggacagat
     8041 aattccacta cagccacatt ctcagcaatc acgaccgccg gaatgtttct cggtgcgctg
     8101 gtgggcggca tcattggcga caaaaccggt cgcagaaatg cgttcatcct ctatgaggcc
     8161 attcatattg cctcgatggt tgtcggtgct ttctcaccga atatggattt tcttattgcc
     8221 tgccgttttg tgatggacgt tgggctggga gctttactgg ttacactgtt tgctggtttc
     8281 accgaatata tgcccggtag aaatcgcgga acgtggtcca gtcgggtttc ctttattggc
     8341 aactggtcat atccgctctg ctcactgatt gcgatgggac tcacgccgct gattagtgca
     8401 gagtggaact ggcgggtaca actgcttatt cctgcaatat tgtcgcttat cgctacggcg
     8461 ctggcctggc gctactttcc tgaatccccg cgctggctgg aatcgcgcgg acggtatcag
     8521 gaagccgaga aagtcatgcg gagtatagaa gaaggcgtca tacgccagac gggtaaacct
     8581 ttgccgcctg tggttattgc tgatgacggt aaagcgccac aagcggtgcc gtattcagcc
     8641 ttactgacag gagtattact gaaacgcgtg atattaggct cttgtgtgct gattaccatg
     8701 aacgttgtgc agtacacact aattaactgg ttgccaacaa tattcatgac ccaggggatt
     8761 aatttaaaag actcgattgt tttaaatacc atgagtatgt ttggcgcgcc atttggtatt
     8821 tttattgcca tgctggtgat ggataaaatt ccgcgtaaaa caatgggtgt ggggctatta
     8881 atcctgattg cggtgctcgg atatatctat tcactgcaaa ccagtatgtt gctcataacg
     8941 ctgattggtt tcttcctgat tactttcgtc tatatgtacg tttgctatgc ctcggcagtg
     9001 tatgtccctg aaatctggcc gacagaggcc aaactccgtg gctccggtct ggcgaatgcg
     9061 gtagggcgaa tcagtggtat tgccgcacct tatgccgttg cagtgctgct cagtagttat
     9121 ggtgtaacgg gagtctttat tcttctgggg gctgtttcaa atattgtcgc aattgctatc
     9181 gccaccattg gaattgaaac caaaggtgtc tccgttgaaa gtttaagtat tgatgcagta
     9241 gccaataaat aatacgggtg gattcaaaat gaaaaattca aaagcaatat tgcaggtgcc
     9301 gggcacaatg aaaattattt cagcagaaat accagtgcct aaagaagatg aagttttgat
     9361 taaagtagaa tatgtcggta tttgtggttc agatgtacat ggttttgaat caggcccgtt
     9421 tattccgcct aaagacccaa atcaagaaat tggcctgggt catgaatgcg ccgggacggt
     9481 tgtggctgtg ggaagccgcg tgcgcaaatt taaaccgggg gatcgggtaa atatcgaacc
     9541 tggcgttcct tgcggtcact gtcgttactg tctggaaggc aaatataaca tctgcccgga
     9601 cgttgatttt atggcgacac aacccaacta ccgcggcgca ttaacgcact atctgtgtca
     9661 tccggagagc tttacttaca aactgcccga caatatggac acgatggaag gggcgctggt
     9721 ggagcctgcc gcagtcggga tgcatgccgc gatgctggca gatgttaaac cgggtaagaa
     9781 gataattatt ctgggagcag gttgtattgg tttgatgacg ttgcaagcgt gcaaatgcct
     9841 gggcgcaacg gaaattgccg tcgttgatgt actggaaaaa cgtctggcaa taggggaaca
     9901 gcctggtgcg acagtggtta ttaatggcgc aaaagaggac actattgcac gctgtcagca
     9961 atttaccgaa gacatgggcg cagatattgt tttcgaaaca gcgggttctg cggtcaccgt
    10021 taaacaggca cctttaatct ggtaatgcgc ggcggtaaaa ttatgattgt tggtactgta
    10081 cccggcgatt cggcaatcaa tttcctcaaa atcaatcgcg aagtcactat ccagacggta
    10141 ttccgctatg ccaatcgtta tccggtcacg attgaagcta tttcttcagg gcgattcgat
    10201 gtgaaatcga tggtgacgca tatttacgat tatcgggatg tacaacaggc atttgaagag
    10261 tcagttaaca acaaacgcga cattattaaa ggcgttatta aaattagcga ttaattaata
    10321 cgggagaaca gaacatgctc gcagatatca gatattggga aaacgatgcc acaaataaat
    10381 attatgcaat tgcccatttt aacgtatgga atgcagaaat gttgatgggc gttaaagacg
    10441 cagccgaaga agcgaaatcc ccggttatta tttcttttag tacaggtttt gtcggtaaca
    10501 cctcatttga agatttctct cacatgatgg tatcaatggc acaaaaagca acggtgccgg
    10561 taataactca ttgggatcat ggtcggagta ttgagattat tcataacgcc tggactcatg
    10621 gtatgaattc attaatgcgt gatgcttctg catttgattt cgaagaaaat attcgtttaa
    10681 ccaaagaggc tgttgatttc tttcatccgc tgggtattcc ggtagaggcg gaattagggc
    10741 atgtcggtaa tgaaaccgtt tatgaggaag cgctggcggg ctatcactat accgatcctg
    10801 accaggcggc tgaatttgtt gaacgtacgg gctgtgactc attggctgtc gccatcggca
    10861 accagcatgg ggtttatacg tcagagccgc aattgaactt tgaggtcgtc aaacgcgtac
    10921 gcgatgctgt ttctgttccg ctggttttgc atggcgcatc ggggatcagt gatgccgaca
    10981 ttaaaactgc aatttcgttg ggtattgcga aaatcaacat tcatacggag ctctgtcagg
    11041 ccgcaatggt agcagtgaaa gaaaaccagg atcagccttt cctgcattta gaacgtgaag
    11101 tacgtaaagc tgtaaaagaa cgggcattgg aaaaaattga actgttcggt tctgatggca
    11161 aagcggaata atgatatgga taatctcgac gttatttgta taggtgccgc tattgttgat
    11221 attccattac aaccggtcag taaaaatatc tttgatgtag attcttatcc tcttgaaaga
    11281 atcgcaatga gcaccggggg cgatgcaata aatgaagcaa caattatttc tcgtctgggg
    11341 catcgtaccg cattaatgag ccgcattggt aaagatgcct cagggcaatt tattctcgac
    11401 cattgtcgca aagagaatat tgatattcaa agcctgaaac aggacgtcag catagataca
    11461 tctattaacg ttggactggt gacggaggat ggcgagcgga cgtttgtcac gaaccgtaac
    11521 ggcagtttgt ggaaattaaa tattgacgat gttgattttg cgcggtttcc tcaggcgaaa
    11581 ttattatcac tggccagtat tttcaacagt cctctattgg atggtaaagc gctaacagaa
    11641 atttttacgc aagcgaaagc ccggcagatg atcatttgtg ccgatatgat caaaccgcgg
    11701 ttgaatgaaa cgctggatga tatttgcgaa gcattgagtt atgtcgatta tctgtttcca
    11761 aattttgccg aggaaaaatt actcacaggg aaagagacac tggatgaaat tgctgactgc
    11821 tttcttgcgt gcggcgtaaa aacggtggtg attaaaacgg gtaaagacgg ctgctttatc
    11881 aagcgtggtg acatgacgat gaaggtgccg gcggtcgcag gaataaccgc catcgacacc
    11941 attggcgcgg gcgataactt tgcttcaggt tttattgcgg cactgttaga aggcaaaaat
    12001 ctgcgtgaat gcgcacgctt tgccaatgca acggcggcta tctcggttct aagcgtcggt
    12061 gccaccaccg gcgtaaaaaa cagaaagctg gtcgaacaat tgctggaaga atacgaagga
    12121 taatgaaggc aaatgaaaaa gataccttta ggcacgacgg atataacgct ttcgcgaatg
    12181 gggttgggga catgggccat tggcggcggt cctgcatgga atggcgatct cgatcggcaa
    12241 atatgtattg atacgattct tgaagcccat cgttgtggca ttaatctgat tgatactgcg
    12301 ctaggatata actttggcaa tagtgaagtt atcgtcggtc aggcgttaaa aaaactgccc
    12361 cgtgaacagg ttgtagtaga aaccaaatgc ggcattgtct gggaacgaaa aggaagttta
    12421 ttcaacaaag ttggcgatcg gcagttgtat aaaaaccttt ccccggaatc tatccgcgaa
    12481 gaggtagaag ccagcttgca acgtctgggt attgattaca tcgatatcta tatgacgcac
    12541 tggcagtcgg tgccgccatt ttttacgccg atagctgaaa ctgtcgcagt gcttaatgag
    12601 ttaaaggctg aagggaaaat tcgcgctata ggcgctgcta acgtcgatgc tgaccatatc
    12661 cgcgagtatc tgcaatatgg tgaactggat attattcagg cgaaatacag tattctcgac
    12721 cgggcaatgg aaaacgaact gctgccgcta tgtcgtgata atggcattgt ggttcaggtt
    12781 tattccccgc tagagcaggg attgttgacc ggcactatca ctcgtgatta cgttccgggc
    12841 ggcgctcggg caaataaagt ctggttccag cgtgaaaaca tgctgaaagt gattgatatg
    12901 cttgaacagt ggcagccact ttgtgctcgt tatcagtgca caattcccac tctggcactg
    12961 gcgtggatat taaaacagag tgatttaatc tccattctta gtggggctac tgcaccggaa
    13021 caggtacgcg aaaatgtcgc ggcactgaat atcaacttat cggatgcaga cgcaacattg
    13081 atgagggaaa tggcagaggc cctggagcgt taaatactca acacgattca cctctgtttc
    13141 tgtgaaaact gtgatgaaaa tctgtgattc aaataggtta ttttgaaagt aaacatcggt
    13201 tatgcgataa tcgcgctaat gtgatgtaaa aagatacagg ggtgtaatcg tggcggcaaa
    13261 agacaggatt caggcaatta agcaaatggt tgccaacgat aaaaaggtga cagtctcaaa
    13321 tttgagtggg atttttcagg taaccgaaga aaccattcgc cgcgatcttg agaagctgga
    13381 agatgaaggc tttttgacca gaacctatgg tggtgctgtt ttaaatacag cggtgttgac
    13441 ggagaatatc catttttata agcgcgcttc atcgttttat gaagagaagc tgcttattgc
    13501 acgcaaggca ctacccttta tcgacaataa aaccaccatg gcagccgatt ccagtagtac
    13561 cgtaatggaa ttgctcaaat tgttacagga ccgtagtgac ctgacgttgc taaccaactc
    13621 ggcggaagcg attcatgttc tggctcagtc agaaattaaa gtcgtttcaa cgggtgggga
    13681 actaaacaaa aatacacttt ccctgcaagg aagaattact aaagagatca tcagccgcta
    13741 ccatgtcgat atcatggtaa tgagttgtaa aggtcttgat attaacagcg gcgcgctgga
    13801 ctctaatgaa gcggaagctg aaatcaaaaa gacaatgatc cgtcaggcga cagaagttgc
    13861 gttattggtt gatcactcta agtttgatcg taaagctttt gtccagttag ctgactttag
    13921 tcatattaat tatataataa ctgataaatc accgggtgca gagtggatag cattttgcaa
    13981 agacaataat atccagctgg tgtggtaatt taagcacacg ttgtcaatat tatttgttcc
    14041 tgcgactgga aataaaatga tgataaagat gcggcaagtg aatttgccgc aattgctacc
    14101 ttttatagca aatccgaggc acccaatgga acaatatgat caaattggcg caagactgga
    14161 ccgcttgcct ttggcccggt ttcattatcg tatatttggt ataataagct ttagtctgtt
    14221 attaacgggg tttttgagtt actcaggtaa tgtcgtctta gcaaagctgg taagcaatgg
    14281 atggtcaaat aatttcctct atgccgcctt tacctcggca ttaatgtttg gttatttcat
    14341 cggctcactt actggtgggt ttattggtga ctactttggg cggcgcaggg cgtttcgcat
    14401 aaatcttctc atcgtcggta ttgctgcaac aggggccgct tttgtccctg atatgtactg
    14461 gctcattttc tttcgcttcc tgatgggaac aggaatgggg gcgctgatta tggttggcta
    14521 tgcctcattt acggagttta tccccgcgac ggtgcgtgga aaatggtccg cgcggctctc
    14581 atttgttggt aactggtcgc ccatgctgtc tgcggcgata ggcgtggtgg ttatcgcttt
    14641 ttttagttgg cgaataatgt ttcttctggg tggtattggc atactgttag cctggtttct
    14701 ctcaggtaaa tactttatcg agtcgccacg atggctggaa gggaaagggc aaatcgccgg
    14761 tgcagaaagc caacttcgtg aagtagagca gcaaattgaa agggagaaga gtattcgttt
    14821 accccagctt actttgaacc agagcaacag caaggttaag gtaatcaagg gtaccttcag
    14881 gctcctgttt aaaggggaaa tgttacgacg tacattagtt gcgataactg ttttaattgc
    14941 aatgaacatt tcgctttata ccattaccgt atggataccg accatatttg ttaactccgg
    15001 cattgatgtc gataaatcaa tattaatgac cgctgttatt atgattggtg ctccggtagg
    15061 aatatttatt gcggcattaa ttattgatca ttttcctcgt cgattatttg gctccgcctt
    15121 acttattatt attgccgtgt taggctatat ctattcaagt cagactacag agtgggcgat
    15181 tttaatctat ggtctggtga tgatcttctt tttatacatg tatgtttgct tcgcgtcggc
    15241 ggtttatatc ccggagcttt ggccaacgca tttacgcctg cgcggttcgg gtttcgttaa
    15301 tgccgtcgga cggatcgtcg cagttttcac gccctatggc gttgcggcat tattaacaca
    15361 ttatgggtcg atcacggtgt ttatggtgct tggtgtcatg ttagtgctct gtgcgctggt
    15421 tctctccatt tttggcatcg aaacgcggaa ggtgtcgctg gaagagattt ctgaggtgaa
    15481 ttaaatcttt cgttgatggc atcaattgcc agatgtacca ctgacgtgtt ttatttggcc
    15541 tacaatgcca gtacagaatt gtaggccagc gtaaaattac ccctgcgtct cttcccagtc
    15601 tgccagcgta tatagcgttg ccccagctgc tgacatctcc ataaacgcgt gtgcactgtc
    15661 ctggggctgg atattcacgc cacgacagcc atcggtaatc acgtttacct tataacctaa
    15721 ctgtaacgcg tccagcacgg taaacttcac gcaatagtca gtagccaggc ccataacgat
    15781 caattcatcg atttcatgat cgcgcaacca gtcatcaagc gcggttttct gccgacgtcc
    15841 gttatcaaaa aaggcactgt aactgtcaac taaaggattt tcgcctttat ggaacaccgc
    15901 tgcgatctct ttttgattca gtaacggatg taattgtgcg ccttcactgt tctgcacaca
    15961 gtgttcaggc cagaaagttt gccgcaggcc gccgagttgg cctggcgtat aaggctctac
    16021 gccgtgctgg ctggcaaagc tgccgtgatt cgccggatgc cagtcctggc tggcgataac
    16081 cgcttctccg cgcgactggc accagtcaat aaggcggtta gcaacctcca ccgtactgtc
    16141 accttccggt acggcgagcg cgccaccagc acagaaatca ttttgtaaat cgaccagtaa
    16201 cagggcgcga tggggcattg cagtctcctt aatcatccgg cgtcagttcg ccgcgcaggt
    16261 tttggctcat ggccttgcga atggtttcag tatccagttc ctggctcagc aggtaatgca
    16321 gtttggttag cgtggcttct acagtcatat ctgcaccgtc aataacgccg gcatgggcga
    16381 gggcgttacc ggtggcataa ccactcatat tcactttacc ggacatacat tgtgtcaggt
    16441 tgaccaccac aataccgcga tcgctggctt cttgtaattc ctgcaggaag gctttgtttt
    16501 gtggcgcatt acccacgcca taggagcgca gaatcaatgc tttcaccggt tggcgcagaa
    16561 aattgcgcac gacgtcagca gaaatccctg gataaatcgt caccacgcca attggttgtg
    16621 gcgtgattgg atgaacgatt aattcccctt caccgtgcgg ggcgggtggc gtattcaaac
    16681 gacgaatatg gataccagct tccagtaacg gaggaaggtt tggagaggca aacgcatcaa
    16741 aaccatcggc atgggctttg gtagtgcggt tgccgcgata caatcggtta ttgaaaaaga
    16801 gcgttacttc gttgatcgga taattcggcg caacgtacaa cgcattcagc agattaattt
    16861 gtccgtcaga gcgtaactca gccagcggga tttgtgaccc tgtcacaatg accggtttac
    16921 cgagattctc gagcatgaac gacagcgcag aggcggtata cgccatcgtg tcggtaccgt
    16981 gtagaatgac aaaaccatca tagtcgtcat agtgcgcttt aatatcttca gcaatatgct
    17041 gccagtcttc cggcgtcata tcagaagaat ccatcaacgg cgtatattca tgaatggtga
    17101 aatctggcat ctccgggcga tggaattccg gcatcagcgc cagttggcgt tgtagatgac
    17161 ctgacaccgg aatataaccc tgctcggaac gctgcatccc gatggtcccg cccgtgtagg
    17221 cgacgtaaat tgatttcttt tgcatgatat tgatatgaat gatatcgaaa gagcaaaagt
    17281 atatacgtga ggcaggagga gcgcacctga tactatgcgg ataaggaaat atggggcgag
    17341 acagcgggaa gaggattcgt cgaggtaaaa aataatgtgg ccgacgatct gtcggccact
    17401 cagtacaaga cttaacgcac gttggcgcag gtcaggcaaa acgcataacg gttttgtggg
    17461 tcgttaaacg cggccagctt gtcgctttca cttttaacgg tagaggctac cgaggcaagc
    17521 ggtgcaggta acatggcctg gaacgcatct ggcaacattg cccggacaga accagacatg
    17581 ttgtccatca ctttgtcgaa gaaggtcggt tcatcaacgt agtattccag atgccactgt
    17641 ttcacttttg cgagctctgc tgctttggca accgcatcat cgaaatcccc gagactatcg
    17701 accagcccgt tagcttttgc atcctgaccg gtccagacgt ggccctgggc gattttatca
    17761 atttgctccg gcgtcgaatg acgcgcatca gcaaccagcg tgataaagcg tttatagcca
    17821 ttctcaatgc ttaattgcat catctgctgc gcttccggcg gcagtgccct ggtgatagaa
    17881 acatccgcca gcggtgaagt tgagacacca tcagtatgaa caccaatcga atccagacta
    17941 ttttctacgg tggtgatcac gccgaagata ccgatagaac cggtcagggt gctggggtta
    18001 gccacaatgt aattagctgg cgtggaaatc cagtaaccac cagatgccgc catgccgccc
    18061 atcgatacaa ccacagactt acccgctgcc cgggctgctg ccagttcagc gcgaatcact
    18121 tcagacgcgg taacgctgcc gcctgggcta ttaacacgca ggacaatcgc tttcactttc
    18181 gggtcaaggc gagcgtcgcg gatttgtgcc gcagtggtat caccgccaac attcccctga
    18241 gtttcctcgc catccataat tgcgccatta gcgaagacga caccgatgct gtcaccggta
    18301 tctgccggcg ttttcaatgc gtaatcgtaa taactgatgg cgcgataatt tttatcagtc
    18361 ttactccagc cgaactcttt ggtcagagct ttttcgattt cggcactcga tgccagtgca
    18421 tcgaccagct tgttttccag tgcatatttc gcggtatcgc caccggtttt ggttaaaccc
    18481 tcaagcaacc cttgcgcgcc agggaatacc tgctgagcag ggatctgccg gttagcggca
    18541 acagtattca gatagttttg ccacagctca ccaatccagc ggctgtcagc ttcgcgggct
    18601 gccggtgaca tatcatcacg aataaacggt tcaacggcag atttatacgt acccacgcgg
    18661 aacacatggg tggaaacttt cagcttatcc agcaacgatt tgtagtacag accgttggta
    18721 gcaaaaccgt gcagatcaac cacgccttgc ggtgacagcc aaattttatt ggcgaaactg
    18781 gcgagataat attgcccctg gctgtagttc tcgccaacgg cataaaccgg tttcccgctg
    18841 tcacgaaact ctttcagggc tttgccgatg tactgcatag acggctggtc gccgcctgcg
    18901 aagtttttca gatccatcac gataccggtg atattgcggt cgtccttcgc ctggcgaatg
    18961 gtgttgacga tatcaaacag tgagttttcc tgcagacgat cggaactggc accaagcagc
    19021 tggcggctta atttactaaa ccgctgagaa ctgtctggtt tatcgacgat cacaccagaa
    19081 atgtccagca gcagtgcgcc acgactggcc gtttctttcg aatcaccacc actgacctgc
    19141 atccagatcc ccacaccaac cagtacgagg aaaataaaga acaggttaag taccatttca
    19201 cggacgaaat tcagcagacg ccacgtccat ttaaaaaatc cggcaataaa tcgccaaagg
    19261 gttcgcatgt attctcccaa cttaaggtca cacctgtcac aggcgcaata gatgtggtta
    19321 tcgtaatgac ccaaccggca cttgtcagca ggaattgcgg actgtgctgt aacaaaatcc
    19381 actctcgtgt taattttgtg aataaatatc acgacaggag ttaatcaaat ggatgcactc
    19441 gaactattga tcaatcgccg tagcgcctcc cgcctggctg aacccgcgcc aacgggtgaa
    19501 caactgcaaa acatcctgcg tgcgggtatg cgtgcgccgg accataagtc catgcaaccg
    19561 tggcattttt ttgtgattga aggggaaggg tgcgagcgtt tcagcgccgt actggaacag
    19621 ggggcgattg ctgccggtag tgatgataaa gctatcgaca aagcccgtaa tgcgccgttc
    19681 cgcgcaccgc tcatcatcac ggtggtggcg aaatgcaaag aaaatcataa agtcccgcga
    19741 tgggaacagg aaatgtctgc cggatgcgcg gtcatggcga tgcaaatggc agccgttgcc
    19801 cagggatttg gcggcatctg gcgcagtggc gcattaactg aaagtccggt agtgcgtgaa
    19861 gcattcggtt gccgtgagca ggataaaatt gtcggttttc tctacctcgg cacgccgcag
    19921 ttgaaagcat ctacgtcgat taacgtcccg gacccgacgc cgtttgtaac ttatttctga
    19981 taattgtcgc gaaactgtct ggattgtgag caaaggcgca ggaattcaga cactctcact
    20041 tatcacttca cggaatgagg gttaccatac ccggattgca ttgacaggag atgtccatga
    20101 gcgagaactc gattcgtttg acccaataca gccacggagc tggttgcggc tgtaaaattt
    20161 ccccaaaagt gttggaaacc atcctgcaca gtgagcaggc gaagtttgtt gatccgaatt
    20221 tgcttgtggg taatgaaacc cgcgacgatg cggcggtgta cgatctgggc aatggcacca
    20281 gcgttatcag taccaccgac ttctttatgc cgatcgttga taatcctttc gattttggcc
    20341 gcattgcggc gactaacgcc atcagcgata tcttcgcgat ggggggcaaa ccgattatgg
    20401 cgattgcgat cctcggctgg ccgattaaca aactttcccc ggaaattgcc cgcgaagtaa
    20461 ccgaaggtgg acgctatgca tgccgtcagg cgggtattgc gctggctggc ggtcactcca
    20521 tcgatgcgcc ggagccgatt tttggtctgg cggtaacggg gatcgtaccg accgagcggg
    20581 tgaagaaaaa cagtaccgca caagccggat gcaaactgtt cctgacgaaa ccgctgggga
    20641 tcggcgttct taccacggct gagaaaaaat cactgttgaa accagaacat cagggactgg
    20701 cgacggaagt gatgtgccgg atgaacatcg caggcgcgtc ctttgccaac atcgaaggcg
    20761 taaaagcgat gaccgacgtt acgggctttg gtctgctggg ccacttgagc gaaatgtgtc
    20821 agggggctgg tgtgcaggca cgcgtcgact atgaagcgat cccgaaactc cccggtgttg
    20881 aagagtacat taagttgggc gcagtacctg gcggcactga acgtaacttt gccagctacg
    20941 gtcatctgat gggtgaaatg ccgcgtgaag tgcgcgatct gctgtgcgat ccgcaaactt
    21001 ctggcggttt gctgctggcg gtcatgccgg aagcagaaaa tgaggttaaa actacagccg
    21061 ccgagtttgg cattgaactg acggcgattg gcgaactggt gccagcgcgc ggcggtcgtg
    21121 ccatggttga gatccgttaa tttaatgcgg ttgtttattg ccgaaaaacc gagtctggcg
    21181 cgcgccattg ctgatgtcct gcccaaaccg caccggaaag gcgatggctt tatcgagtgc
    21241 ggtaatggtc aggtggtgac ttggtgtatc ggtcacctgc ttgagcaggc gcagccagac
    21301 gcctacgaca gccgctatgc gcgctggaat cttgcggatt tgccgattgt cccggaaaag
    21361 tggcaattac agccacgacc ctccgtgacc aaacaactta acgtcatcaa acgcttcctg
    21421 catgaagcca gcgaaatcgt tcacgccggg gacccggatc gtgaagggca attgctggtg
    21481 gatgaagtgc tggactatct gcaactggca ccggaaaagc gccagcaggt gcagcgttgc
    21541 ttgataaacg acctgaaccc gcaggcggtt gagcgggcga tcgaccgtct tcgttccaac
    21601 agtgagtttg tgccattgtg cgtttctgcg ctggcgcgag cgcgtgccga ctggctgtac
    21661 ggcatcaata tgacccgtgc gtataccatt ctcggtcgca atgccggtta tcagggcgta
    21721 ctttccgtgg gacgcgtgca gacacccgtg ctcgggctgg tggtgcgccg cgatgaagag
    21781 attgaaaact tcgtggcgaa agacttcttt gaagtcaaag cacatatcgt gacacctgcc
    21841 gatgagcggt ttaccgctat ctggcaaccg agcgaagcgt gtgaaccgta ccaggatgaa
    21901 gaagggcgct tgttacatcg tccactggcg gagcatgtgg ttaaccgcat tagtggtcaa
    21961 ccggctattg tcaccagcta taacgataaa cgggaatcag aatccgcgcc gctgccgttt
    22021 tcgctttcgg cgttgcagat tgaagcggca aaacgcttcg gtctgagcgc gcagaacgtg
    22081 cttgatatct gccagaagct gtacgaaacg cacaagctaa tcacttatcc gcgttccgat
    22141 tgtcgctatt tgccagaaga acattttgcc ggacgccacg cggtgatgaa tgccatcagc
    22201 gttcatgcac cggatctgtt gccgcagcca gtggtagatc cagatatacg caaccgctgt
    22261 tgggatgaca aaaaggtcga tgcgcaccac gccatcatcc cgaccgcacg gagttctgcg
    22321 atcaacctga cggagaacga agcgaaggtc tataacctga ttgcccgtca atacctgatg
    22381 cagttctgcc cggatgcggt gttccgcaag tgtgttatcg aactggacat tgccaaaggc
    22441 aaatttgtcg ctaaagcccg ttttcttgct gaagcaggct ggcgcacgct gttaggcagc
    22501 aaagagcgcg atgaagaaaa cgacggcacg ccattgcctg tggtggcgaa aggcgatgag
    22561 ttgctgtgtg aaaaaggtga agtggtagag cggcaaaccc agccgccgcg ccattttacc
    22621 gatgcaacac tgctttcggc gatgaccggg atcgcgcgct ttgtgcagga caaagatctg
    22681 aaaaaggtcc ttcgtgcgac cgatggtctg gggacagaag caacccgtgc cgggattatt
    22741 gaactgctgt tcaagcgtgg ttttctgacc aaaaaagggc gctatatcca ctccaccgac
    22801 gccggaaaag cgttattcca ttcgctgcca gaaatggcga cgcgaccgga catgaccgcg
    22861 cactgggaat cggtgctgac gcaaatcagc gaaaagcagt gtcgctatca ggactttatg
    22921 cagccgctgg tggggacgct atatcagctt attgatcaag ccaaacgtac gccggtgcgg
    22981 cagtttcgcg gcattgtggc tccgggcagt ggtggcagtg ctgataagaa aaaggctgca
    23041 ccgcgtaaac gtagtgcgaa aaaaagtccg ccagcagatg aagccggaag cggggcgata
    23101 gcgtaagcga gcgaatcttt cgtgctattc gagtcatatt ctgaaatatc cagcggatca
    23161 agaaagttcg ttggatattt ttttgcatgg ataaaattat cgcctctaaa gtatgtaata
    23221 acagggaatg tgatgaaaaa agttttattg caaaaccatc ctgggagcga gaagtattct
    23281 tttaatggct ggggaatatt taatagtaat tttgaacggg tgattaaaga aaataaggcc
    23341 atgctgcttt gtaagtgggg gttttattta acatgtgttg tcgctgtaat gtttgtattc
    23401 gcagcgataa catccaacgg tttgaatgaa agaggcctga ttaccgcggg atgctctttt
    23461 ctttatctat taattatgat ggggcttatt gttcgggccg gttttaaagc aaaaaaagaa
    23521 caactgcatt attatcaggc taaaggtatt gagccgctca gtatcgagaa gttacaggcg
    23581 ctacaattga tcgcacctta tcgattctat cataagcaat ggtctgaaac gctagagttc
    23641 tggccgcgaa agcctgaacc tggcaaagat accttccaat atcatgtgct tccttttgac
    23701 tcgatcgata tcatcagtaa aagacgcgag tctttagaga atcaatgggg tatcgaagat
    23761 agcgaaagtt attgtgcctt aatggagcat tttctttctg gcgaccatgg agccaatacc
    23821 tttaaagcaa acatggagga agccccagag caggttatcg ccttgttgaa taaatttgct
    23881 gtttttccct cagactatat ctctgattgc gctaataatc gctccggtaa atcctcggcg
    23941 aagctaatat gggcggcgga attatcatgg atgatctcga tatcaagcac agcttttcaa
    24001 aacgggacaa ttgaagaaga actggcctgg cattatataa tgcttgcttc tcgaaaggcg
    24061 cacgagttgt tcgaaagcga agaagattat caaaaaaata gtctaatggg atttctttac
    24121 tggcatatct gctgctatcg cagaaagtta acggatgctg aacttgaagc gtgttatcgt
    24181 tacgacaagc agttttggga gcactacagt aagaaatgcc gttggcccat aagaaatgtt
    24241 ccctggggag catcatccgt taaatactca taaacgcctg aaattttgcc gggggcgctg
    24301 cgcttgaccg gcctacatta gagcgtaact gcaatgaatt gtgcccatct gtaggcctga
    24361 taagcgtagc gccatcaggc atttacaact taaatcacac cctgcgccag catcgcatcg
    24421 gcaaccttca caaaaccggc aatgttcgcg ccctgcacgt agttggtttg ctcaccttca
    24481 ccaccatgct caacacaggc atggtggata tccagcatga tgtgatgcaa acgtgcgtca
    24541 actttctcgg ctttccagcc caggcgcgca gcgttttgtg ccatttccag gcccgacgtt
    24601 gctacaccgc cagcattagc cgctttaccc ggtgcaaaca gtacgcctgc ctgctggaac
    24661 agttcagtcg cttcgatggt ggtcggcata tatgcccctt cagcgacggc tttaacgcca
    24721 ttggcgataa gctgatgcgc ggcgtcaaca tccagttcgt tctgggtggc gcaaggcagg
    24781 gcgatatcaa ccggtacaga ccacggctgt tggccttcga gatagaccag accaaattct
    24841 ttggcgtaat ctgccgctcg accatcgcgg ctggctttga tttcgataag acgtgccagt
    24901 ttctctttcg tgaatccgct tt
//
LOCUS       DAJTYM010000059        24164 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTYM010000059 DAJTYM010000000
VERSION     DAJTYM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(3..575)
                     /locus_tag="OO575_002415"
     CDS             complement(3..575)
                     /locus_tag="OO575_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6665344.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            793..1653
                     /gene="tesB"
                     /locus_tag="OO575_002416"
     CDS             793..1653
                     /gene="tesB"
                     /locus_tag="OO575_002416"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6665345.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1702..2988)
                     /gene="amtB"
                     /locus_tag="OO575_002417"
     CDS             complement(1702..2988)
                     /gene="amtB"
                     /locus_tag="OO575_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6665346.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3018..3356)
                     /gene="glnK"
                     /locus_tag="OO575_002418"
     CDS             complement(3018..3356)
                     /gene="glnK"
                     /locus_tag="OO575_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6665347.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3537..5318)
                     /locus_tag="OO575_002419"
     CDS             complement(3537..5318)
                     /locus_tag="OO575_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6665348.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5311..7083)
                     /locus_tag="OO575_002420"
     CDS             complement(5311..7083)
                     /locus_tag="OO575_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6665349.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7113..7571)
                     /gene="decR"
                     /locus_tag="OO575_002421"
     CDS             complement(7113..7571)
                     /gene="decR"
                     /locus_tag="OO575_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6665350.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7725..8543)
                     /gene="cof"
                     /locus_tag="OO575_002422"
     CDS             complement(7725..8543)
                     /gene="cof"
                     /locus_tag="OO575_002422"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6665351.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8643..10335
                     /locus_tag="OO575_002423"
                     /pseudo
     CDS             8643..10335
                     /locus_tag="OO575_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10400..11095
                     /gene="queC"
                     /locus_tag="OO575_002424"
     CDS             10400..11095
                     /gene="queC"
                     /locus_tag="OO575_002424"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6665352.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11147..11545)
                     /gene="fadM"
                     /locus_tag="OO575_002425"
     CDS             complement(11147..11545)
                     /gene="fadM"
                     /locus_tag="OO575_002425"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6665353.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11639..12010)
                     /locus_tag="OO575_002426"
     CDS             complement(11639..12010)
                     /locus_tag="OO575_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6665354.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12161..14032)
                     /gene="ppiD"
                     /locus_tag="OO575_002427"
     CDS             complement(12161..14032)
                     /gene="ppiD"
                     /locus_tag="OO575_002427"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6665355.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14224..14496)
                     /gene="hupB"
                     /locus_tag="OO575_002428"
     CDS             complement(14224..14496)
                     /gene="hupB"
                     /locus_tag="OO575_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6665356.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14705..17059)
                     /gene="lon"
                     /locus_tag="OO575_002429"
     CDS             complement(14705..17059)
                     /gene="lon"
                     /locus_tag="OO575_002429"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6665357.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17247..18521)
                     /gene="clpX"
                     /locus_tag="OO575_002430"
     CDS             complement(17247..18521)
                     /gene="clpX"
                     /locus_tag="OO575_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6665358.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18647..19270)
                     /gene="clpP"
                     /locus_tag="OO575_002431"
     CDS             complement(18647..19270)
                     /gene="clpP"
                     /locus_tag="OO575_002431"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6665359.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19517..20815)
                     /gene="tig"
                     /locus_tag="OO575_002432"
     CDS             complement(19517..20815)
                     /gene="tig"
                     /locus_tag="OO575_002432"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6665360.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21159..21476)
                     /gene="bolA"
                     /locus_tag="OO575_002433"
     CDS             complement(21159..21476)
                     /gene="bolA"
                     /locus_tag="OO575_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6665361.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21781..22359
                     /locus_tag="OO575_002434"
     CDS             21781..22359
                     /locus_tag="OO575_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6665362.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22403..23878
                     /gene="ampG"
                     /locus_tag="OO575_002435"
     CDS             22403..23878
                     /gene="ampG"
                     /locus_tag="OO575_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6665363.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5663 a         6661 c         5818 g         6022 t
ORIGIN      
        1 gcttaatatt gcgtaggagc gggaactgcc gaagacggat tcacctgagt tggtgaagtc
       61 gaaggtacgg tagtcgttgc gccaccgctg gcctgaaccg gcacggcggt ttgctgtacc
      121 ggcaccaatg tcaggtcggc tttagttccg ccctggttga tcaccggctg aacggtatcg
      181 gtgataaata ccagtttgtc attcacggta atcgccgcac tcaacagaat acgcgcgttc
      241 ggctgaacat ctgccgggtt aaatggcaga acaaagctga atggtgactg tttaccttca
      301 gtacgcaccg ctttctgcgc cagcactttt gacggtgcat cggctaacga cgcgtcagaa
      361 agtgtcacgg tcagcacagc atcaggcggc agtgcgactt tctgacggat ccagacagta
      421 ccggagacat tcggttgctg gatagcaggt tgttgtgttg ctgaaataga cgtatttgca
      481 gccggggctg gcgtctgaat atccgcgctt ttatctgcgc aagccgccaa cgcaatcgca
      541 accgctaaac cactggccat gtgcacgagt ttcattcatt tctccttatt atcaatgcac
      601 cagcgggcta actttcctcg ccggaagagt ggttaacaaa atagtaacgt caacaagtgt
      661 ggcacacatc acgcatttct gcctgtaatt agcccgtaat tcagactatt tcacccatcg
      721 gaccagtggc aaatttgcgt tatactcaac tcactttggc ttgctgcggc agctttgtta
      781 ctggagagtt atatgagtca ggcgctaaaa aatttactga cattgttaaa tctggaaaaa
      841 attgaggaag gactctttcg cggccagagt gaagatttag gtttacgcca ggtgtttggc
      901 ggccaggtcg tgggtcaggc cttgtatgct gcaaaagaga ccgtcccgga agagcggctg
      961 gtacattcgt ttcacagcta ctttcttcgc ccaggcgata gtaagaaacc gattatttat
     1021 gatgtcgaaa cgctgcgtga cggtaacagc ttcagcgccc gccgggttgc tgctattcaa
     1081 aacggcaaac cgatttttta tatgacggcc tctttccagg caccagaagc gggtttcgaa
     1141 catcaaaaaa caatgccgtc cgcgccagcg cctgatggcc tgccttcgga aacgcaaatc
     1201 gcccaatcgc tggcgcacct gctgccgcca gtgcttaaag ataaattcat ctgcgatcgt
     1261 ccgctggaag tccgtccggt ggagtttcat aacccactga aaggtcacgt cgcagaacca
     1321 catcgtcagg tgtggatccg cgcaaatggt agcgtgccgg atgacctgcg cgttcatcag
     1381 tatctgctcg gttacgcttc tgatcttaac ttcctgccgg tagctctaca gccgcactgc
     1441 atcggttttc tcgaaccggg gattcagatt gccaccattg accattccat gtggttccat
     1501 cgcccgttta atttgaatga atggctgctg tatagcgtgg agagcacctc ggcgtccagc
     1561 gcacgcggct ttgtgcgcgg tgagttttat acccaagacg gcgtgctggt tgcctcgacc
     1621 gttcaggaag gggtaatgcg taatcacaat taaaaaaata gccggaggtg aaaaccgtcc
     1681 ggctattttt tgcagtgctt gttacgcgtt ataggcattc tcgccgtggc tgttgacgtc
     1741 cagcccttct cgctcttgtt cttccggcac acgtaggcca accgtcagat ccgccagttt
     1801 gtagccaata aatgccacca caccggacca gacgatcgta atggcgatgc tttccaactg
     1861 taccagcaac tgatggccca tcgtcacacc ttcagcgaag cccacgccgc ccagagaact
     1921 ggcggcaaaa atcccggtca tgatacagcc gacaatgcca caaacgccgt gcacaccgaa
     1981 gacatcgcag ggatcatcca cccgcagcaa gcgtttgagc atggtaacgc cccacaaacc
     2041 cgccagacca gctaccacgc cgataatcaa cgcgccgcca accccaatgt agccgcacgc
     2101 tggcgtcacg ccgaccagac cggcaatcgc gccagaacac gctcccagca gagaaggctt
     2161 accacgcagc gcccattcac cgaagatcca gccaagaatt gccgccgccg ttgcgaccac
     2221 agtattcaca aatgccagtg ccgcgatttc attcgccgtg cccactgacc cggcgttaaa
     2281 gccaaaccaa ccgatataga gaatggcagt cccggtgaag accatcggca ggttgtgcgg
     2341 tttaaacgcc tctttaccga agcctacgcg tttgcctatc agatacgcgc ccaccagacc
     2401 ggcgattgct gcgttaatgt gcaccaccgt gccacccgcg aaatccagcg caccgtgaga
     2461 agccagcaaa ccaccgcccc acaccatatg cgcaatcgga atgtaagaga gcgtcagcca
     2521 taccaccacg aaaatcaaca cagctgagaa gcggattcgt tccgccagcg ccccaactat
     2581 caagcctacg gtaatgcagg caaacgatcc ctgaaacgct acgtggatat actgataaat
     2641 gctgcccatc accgccgtca gttcgatgtt ttttagcatc aaccagttaa tgttgccgaa
     2701 gaagttgttg ccctcaccaa acgccagcga ataaccgtaa accacccaga gaatacagac
     2761 cagtgcaaac gtcaccgtca cctgcgtcag catcgacagc acgtttttgc cgcgaatcaa
     2821 cccaccgtaa aacagggcaa tccccggaat agtcataaac agcaccagcg cagtacaaat
     2881 catcataaac gcattgtcgg ctttatcggc caccgcaggt gcagccatta ccagtcccgg
     2941 cagcatcgcc agtgaagcaa gcccagtttt tatcgtcgct atcttcattt ttcgttcctg
     3001 ttgctgtgtg ccagagatta cagcgccgct tcgtcggctt cgccggtacg aatacgaatg
     3061 acgcgttgca attcagcgac gaagattttg ccgtcgccaa tttttccggt gtaagccgcc
     3121 ttactgacga tatcgatcac ttcatcgagt tggtcatcag caatcgccac atcaattttt
     3181 acttttggca ggaagttgac gctgtattcc gccccccggt acagctcggc atgccctttc
     3241 tgacgcccga aacctttcac ttcggtgacg gtcaggccct gaataccaat ggaagataac
     3301 gcttcacgaa cgtcttccag cttgaatggt ttgattatca cggtcaccag cttcatagat
     3361 cccctccggt cagaattcgg taatggttct gctacacgaa gaaggtattg caagcggtgt
     3421 gccagaaatg aaaaacgaga aagagagcag gaagttaaga agattgaaaa tgaaaacgca
     3481 ctatgacagt gcacagtgcg ttacattttt gcaccaacga tgaaatattg cgctattcag
     3541 gcgctcaatg attcctcttc acgcacgctg gctgccagct cttcgcccgc aagttgcagt
     3601 tgatacatct gccagtagcg tccctgggcc gccagcagtt gctggtgagt gccctgctcc
     3661 acggcttgcc cacgatgaag caccagaatg gtgtcggcat caataatagt tgataagcgg
     3721 tgagcaatca ccaccagcgt ggtatgttca cgcaccgccg ccagagcatg ttgaatcgcc
     3781 tgttcagtac cggagtcaat gctggcggtt gcctcatcaa ggatcaggat ttgcggcgtc
     3841 tccaccagca cgcgcgccag tgccagcagt tgcttttgcc cgactgagag attatttccc
     3901 tgctcgccca gcggcgtata aatacaatca ctcatgctgc gcgccagctc cgccagttgc
     3961 acggtttcca gcgcctgcca gacgcgttct tcggagatat cccgccccag cgtcacatta
     4021 gcgaggaagg tatccgccag caccaccgga tcttgctgca ccattgccac gccctggcgc
     4081 agcgcgctat gacttagcga acttaatgga cgaccatcaa ggtgaatctc accttccgtt
     4141 agcgggtaat agcccatcaa taaactggcg agggtacttt taccactgcc ggtatgcccg
     4201 accagcgcca caaagttacg cgaaggcaca gagagattaa tgttctttag caccagattg
     4261 tcatcgcgat aagcaaatga cacgttatcg acttcgatgg tgccactctg taacgggtaa
     4321 tcatcattgc catattgctg gcgcggtccg tccatcagtt caaacacgcg ctcaccagca
     4381 acaacagcct gttgcagcat cgcctgttgc gtggtcagtt cgattaaggg ttcgttaagt
     4441 cgcccaagat agctgataaa cgcatacaac acgcccactt caatggtgcc gctggcggag
     4501 aagccaaaca gcatcaataa gccacaaaga atgagcgatg aaaacagact cagcagcgga
     4561 cgcagcagaa aaccgtcgag gcgcagggtt tgcatcctag ccatatagtg tgaacgactg
     4621 gcctccccca tacgttcgcc aaatcgcgcc tgctgacgaa actgctggat aacgctcatg
     4681 ccattgatga tttcgttaaa gccgtcgttg atatccgcca gataggcgcg cacacggcgg
     4741 acaatcggcg tgctgtaacg ctggtatatc accattacca ccagcaccac cgggaaaatc
     4801 attatcgcca ccagcgccat tcgccagtcg aggctgaaca tcgccaccag catcgcgccc
     4861 accagtgcgg cactgcgcag gacagttgcc actacggtaa cgtagagatc gcggatcact
     4921 tcagtgtcat tagtgacgcg ggaaatcacc tgcccgacgg gttgggtatc aaactcgctt
     4981 aatggctggc gtaacgcagc atccatcacg tcggtacgca actgttgcac tgcgcctact
     5041 gccgcccgat taaacagcag cgactgcgcg taatgtagcc cggcggcaaa cagttgtagc
     5101 ccaacatacg ccgcagccag ccctgcaacc actttcaacg gcaggttatt tttcgctacc
     5161 atattgtcga taaaatagct gataagcagc ggcccactga cttctgccgc cgccgcaacc
     5221 cacatcatca ggaccgcaat ccccagcggt ttacgccacg gcgaaccata cgctaacagg
     5281 cgcttgagag tcggccacag ttggctaaaa ctacgcatcg acggcctcct cgcgaatttc
     5341 cggaacgtcg tcgagcgccg cctccagttg ttgatagcga tacatatcgc gataccaacc
     5401 gctttgttgc gccagctcat catgattgcc acgctgggcg atatgtccgt gctgcatcac
     5461 aataatttca ctggcttccg tcagtgccga aaggcgatgg gcactgatga ttaccgttct
     5521 tccctgtccc cactgacgca ggttatgcag gatctggtgc tctgtgcgtc cgtccaccgc
     5581 cgaaagcgca tcatcaagga tgaggatttc cgcgttgact aataacgctc gagcaatgga
     5641 gatacgctgt ttttgcccac cggaaagcat cacgccgcgc tcgcccactt ctgtatcgta
     5701 accttgcggt agacgcaaaa tatcgtcatg tacgctggct aaccgcgcga catgctcaat
     5761 ctcttgctgg gtggcattcg ggcaacccag cgcgatgtta ttcgccacgg tgtcagaaaa
     5821 aaggaatggc gtctggctga ccaccgccag gcggctacgc cagctatcga gttgtaactt
     5881 cgtcagagga atatcatgaa agcgaatatc cccctcgctg acgtcgaaat gacgctgaat
     5941 gagcgacaac agggtacttt tgccggaacc agtcggcccg caaataccca gcatctgacc
     6001 gggtttcagg gcgaaattga cgttttccag cgcaggatgg tcagtctgcg gatacgtgaa
     6061 ctggtgaata tttacatcca gttcgccacg cccttccggc acgggttcgc taccatcgtt
     6121 caccaccggc gcttccgcca gcatggcgcg aatacggctg tatgcagcac taccacgttc
     6181 cacaatgtta aacatccatg ccagcgccag cattggccaa atcatcagac ctaaatacat
     6241 cataaaactg gtgagctggc ccagcgttaa actgccctgc accaccatcc aactaccgcc
     6301 gccaatcgcc agcaagttcg ccataccaat cgcgatatag atggtcgggt cgaagcgagc
     6361 atcaatacgc gccacccgca tgtttttttt gccggtatct tcggcatccg cggcaaataa
     6421 cgccgactgg cgatcttcca gaccaaaggc tttgatcatg cggatactgg tgaggctttc
     6481 ctgggtgcgg tcattaagac tggaaaacgc cgcctgtgcc agcttaaagc gttcatgcaa
     6541 ggcatcgccg ttgcgcttaa tcatgatcgc catcactggc atcggtaaca gggcaaataa
     6601 ggtcaactgc cagctaattt gcgtagacat cataatcaac acagcgcagc ccattaccag
     6661 tgaatccacc agcgtcagca ccccttctcc ggcggcaaac acgacgcgat cgacgtcatt
     6721 ggtcgcacga gccatgagat cacccgtgcg atgacgcagg taaaactccg ggtgctgccg
     6781 gcttagctga cggtaataat cttcacgcag ttcaacagcc agttggtaag acgcgccaaa
     6841 cagcaatacg cgccagacat aacgcaggag ataaaccaca acggcaatca gcaccatggt
     6901 ggcgatccac atcaggctca gcccggtagt aaagtgttgt tctgtcacgc catcgacaac
     6961 aataccaacc acttttggcg gaaccagttg cagcatcgcg ataatgacaa gcaaggcgac
     7021 agccccaaga tagcgacgcc attcccgacg gaaataccag cttaattgag caaataatcg
     7081 cacgcgttgt gtcctgacct gattctggat atttattcga tgggtaaaga agtggtgtat
     7141 ttaatctgtt ccatcgcgaa gctggaagtg acgtccgaca gccccggcac gctgtttacc
     7201 agacgcttat aaaactcgtc gtagcgtttc atgtcggcaa cctggacgcg catcagataa
     7261 tcgtattcac cagccatgcg ccagaccccc agcacttctg gcatttcggt aaccaccgtg
     7321 acaaagcggc aataccattc gctgctgtga tgttgcgttt ttatcagcac aaaggcggtc
     7381 aggccgaggc ctattttttc cggatccagc agggcgactt tgccgataag gataccgtcg
     7441 tcctccagcc gtttcaggcg cttccagcag ggggtggttg tcagattaac ggcttcagcc
     7501 agtgcctgca aagagagggt gcaatcctgc tgcagtaagg ccagcagctt acggtcaatt
     7561 ttatctaaca tagcccttcc acggagaatt tttttctctt ttaattctat tttaaaggtg
     7621 aaatcgcgac aatttattct gtgcggttta aacaccacgg cacaaaatga caaattcaac
     7681 gcaaaaaaaa accgggcaat tgcccggtgc tggaagggat ctcgttattc gggggaataa
     7741 ggtagatgtg gatagtccag ccagtgcgtc aaatagtgag agacagcctg atttcggcaa
     7801 tgtccaatca ccggtaaatg cgggagctcc gcgcgcagtt gcggcatcgc attgcccata
     7861 ataaacccgc taccgacgct gcctaacatt tcgcgatcgt tcatcgcatc accaaaggcc
     7921 atgcaatcgc gcaacgataa acctaaatgt tgggtccgca ccgtcaatgc agcgccttta
     7981 ttgcagccca ccggcagcac ttcgaggcaa tccgtggcgg aaaaacacaa atgtgcacgc
     8041 tcgcctaatg cttcgtatag ctggatctgc aagcgtgtaa gatcgtcgtg atcgccacag
     8101 aagcagatct tggtgacgct gccgagtggc atttttttga catcgattat ctgataacga
     8161 aaaccgctat agacaaatac ctgcaacaac gcagggctct ctttcccggt aaaccaaccg
     8221 tcgtcattga agatatgcat gctggctcgg gtatcccatt gctgatacag caccagctcc
     8281 gcgacatccg caggtaaatc atcacgatgt aaaagttcac cttccagaga atgcacgcgc
     8341 gttccgttgc cggtaatcaa atatgcatcc agcgatagcg ccccgagaat atgctgcatc
     8401 tccagcgcat gacgccccgt ggcaaaagtg agggtaatgt cgcgttcacg cagtcgcgcc
     8461 aaagtagaga gggttttctc acctaaatga tggtcaggca tcaataaagt gccatccata
     8521 tcaaatgcta ccagacgagc catttctttc tccactctgt gacacggtta atggtgattg
     8581 agtatcacct gatatatacg gaagtaatag tgaatagtta aataatattg ttccgggttt
     8641 atatgcgatt gctcaaccgt cttaaccagt atcaacgtct gtggcaacct tcagccgcaa
     8701 accgtcaccg tcagcgaact ggccgaacgc tgtttttgca gcgaacgcca tgttcgtacg
     8761 ctgttgcgtc aggcacagga ggcgggatgg ctggagtggc aggcgcagtc aggacgcgga
     8821 aagcgcggac aattacgctt tctggtcacg ccggaatcgc tacgcaatgc gatgatggaa
     8881 caggcactgg aaaccggaaa gcagcaagat gtgctggagc tggcgcaact ggccccaggt
     8941 gagctgcgca ctctgttaca gccatttatg ggcggacaat ggcaaaacga tacacccacg
     9001 ttgcgtattc cctactatcg cccgctcgaa ccgctacaac caggcttttt gcccggccgt
     9061 gccgagcagc atctcgccgg gcagatattt tccggcctga cccgcttcga taataatact
     9121 cagcgcccga ttggcgattt agcgcatcac tgggaaacct ctactgacag gttacgctgg
     9181 gacttttatc ttcgttcaac cctacactgg cataacggcg atgcagtaaa agcctcacac
     9241 ttacaccagc gattattgat gctgttacaa ctgccagcac tggatcaatt atttattagc
     9301 gtgaagcgta ttgaagtcac ccatccgcag tgtctgacct tctttttaca tcgccccgat
     9361 tactggcttg cgcaccggct ggcgagctat tgcagccatc tggcgcatcc gcaattccca
     9421 ctgatcggca ctggtccttt tcgcttaaca caattcacag cggagctggt gcgcctggag
     9481 agccatgatt attaccattt gcgtcatccg ctgcttaaag cggttgagta ctggataact
     9541 ccgccgcttt tcgaaaaaga tttgggaacc agttgtcggc atcccgtgca aatcaccatc
     9601 ggcaaaccgg aggagctgca acgggtcagc caagtcagta gtggcatcag tttaggtttt
     9661 tgctatttaa cgttgcgcaa aagtccccga ctctccctct ggcaggcgcg aaaagtgatc
     9721 tccattattc atcaatccgg tttattacaa acgttagaag tcggagaaaa cctgatcacc
     9781 gccagtcatg cattactgcc aggctggact attccgcact ggcaagtacc ggatgaagtc
     9841 aaactaccga aaaccttgac gctggtttat cacctaccga tagaacttca taccatggca
     9901 gaacgcctac aggcgacact ggcagcggaa ggctgtgaac tcacaattat ttttcataac
     9961 gcaaaaaact gggacgacac gaccctactg gcacacgcag acctcatgat gggcgacaga
    10021 ttaattggcg aagcaccgga atatactctg gagcaatggc tacgttgcga tccactatgg
    10081 ccacatgttt tcgacgctcc agcatatgca catctgcaat cgacgctgga cgcggtgcaa
    10141 gtaatgcctg atgaggaaaa ccgatttaat gccctgaaag cggtttttag ccagttaatg
    10201 gcagacgcga cgctgacgcc gctgttcaac tatcactatc gcattagtgc ccctcccggc
    10261 gtgaacggtg tgcgactgac accgcgcggc tggtttgaat ttaccgaagc ctggcttccc
    10321 gcgccgtcgc aatgaatagc tggtccgggt taatcgcagg cgttaccata acgcttttat
    10381 tgttcaagca ggattatcta tgaaacgtgc tgtcgttgtg ttcagtggag gccaggattc
    10441 caccacctgt ctggtgcagg cattacaaca atatgatgaa gtccattgcg tgacgttcga
    10501 ttacggtcag cgacatcgcg cagaaatcga cgtggcacgc gaactggcgc tgaaactggg
    10561 agcacgcgcg cataaggttc ttgatgtaac gctgctcaac gagctggcgg tcagcagcct
    10621 gacacgtgac agcattccgg tgcctgatta tgaacctgaa gccgatggca tcccgaatac
    10681 gtttgtccca gggcgtaata ttttgttcct gacgctggcg gcaatatatg cgtatcaggt
    10741 aaaagcagaa gccgtaatta ccggcgtctg tgaaacggat ttctccggct acccggattg
    10801 ccgcgatgag tttgtgaaag cactaaacca tgccgtcagt ttgggcatgg cgaaagatat
    10861 tcgttttgaa acgccgctga tgtggattga taaagcggaa acctgggcgc tggcagatta
    10921 ttacggcaaa ctggatttag tccgtaacga aacgttgacc tgctataacg gcattaaagg
    10981 cgacggttgc ggtcattgtg cggcatgtaa tttacgcgcc aacggtttga atcattatct
    11041 ggccgataaa ccgacggtga tggcagcgat gaagcagaaa accgggttga ggtaattatt
    11101 ccgggtgtcg ccggatgcgg cttgagcatc cggcaccaca aaacgtttac ttaaccatct
    11161 gctccagctt ttcgcgcaat tccccttcca gagctaatgc tttctgcgtt ttaagatcaa
    11221 tacaaacaaa cgtaataagc gcatccgcta ccacctgccc ttccggctcc agtgtaatga
    11281 cctggtttaa gatgccgctt ttaccgttta attgctgcaa ctgactggta atagttaaca
    11341 ggtcgcttaa taccgctgga cgacgatagt taatattgat attgaccacg acgaaggcga
    11401 tgttatgggc cgtcatccac tgaaaactgt cgctattttc caccccatcc cagcgggctt
    11461 cttcgagaaa ttcaaggtag cgggcgttgt tgacgtgctg gtaaacgtcg agatgatatc
    11521 cacgaacttt gatttgtgtt tgcatagcgc aataacctta cgttgttgtt ataagtacag
    11581 aggtcataac tggtatgacc tcttcagtct ggcaaaaaat tgccactatg caaattaatt
    11641 acagggttaa taccgccaga ttacgttcca ccagcgaatt gcccatcccc ggcacctgct
    11701 ttagatcctc caccgtttta aacggaccgt actcttcgcg ataactgaca atcgcctgcg
    11761 ctttcttcag gccaacgcca ttcatcgcgc gggctagctc ttccgcgctg gcattattaa
    11821 tgctgacccg ggtgccttct tcgtcactgg ctttcgccgg tactgctgct ttactttgtg
    11881 ctgcaggagc ttccgctttg gtttctaccg ccgtcggttt cgccacagaa gctgccgcca
    11941 gcgcgctatg agacattccg gcacaggcca gggacagtgt aatgagcagt gctttaattc
    12001 cgtgtttcat actgttttct ccttgtttgt taacagtgga atcaccttaa cgggagtgaa
    12061 aacagtaaac aaatggcaat gttcagaaat ggaaaaggcc gcgaaagcga ccttttcttg
    12121 ttacagctgc aacgcatttt tttgcgaggc ggcttcagga ttattgctgt tccagcgcat
    12181 cgccaatttt gattttcgcc tctttacgca ggttactcat cagagcttca aagacgattt
    12241 gtgcgttgtt ctgggtgata ccctgcacca tcgctttttt ctgatcttcc ggcattgaac
    12301 cttgtttcac ttcatccagc gccagcagaa ccacattacc ttgcatatcg gtcgccatac
    12361 cgtagctcgg tttgtctttc gctggcagtg gcagtgcaaa cgccgcctgg ctaatcgggt
    12421 cacgaccgga acggcttaag gttttcggct cgccaaattt cagaccggca gcctgcatag
    12481 cttccgcacc tttgccggct ttcaaatcaa ccagcagttt ctcagcatcc actttcgcct
    12541 gttgttcagc tttgttgtgc tgaaccagtg ccttaacttg ttcctgaaca tctgccaacg
    12601 gtttcaccgc ttccggtttg tgctcgctga cgcgcagcac gaatgcgcgg tcgccgtcta
    12661 cggtgatgat gtcagagttg atgcccgacg cgccgttttc acctaccaga ccgccgttaa
    12721 agatagcgtc ggcaaccggc ttgaagttca actcttccgg caggttatct ttgctgaacc
    12781 aacccgtctg agtggcttta acgccggcag cttgctctgc accggccaga gactcggtgt
    12841 cgttgcttgc cgcatcgctc actttctgct gcagcgcata gtacgcatcg agggcttttt
    12901 cgtgtttcac tttcgccgca atgtcgtcac gtacttcgtc taacgatttc actttcgctg
    12961 gctgaatgtc gtccagacgt acaatcagga aaccgaccga agatttgatg acaccagaca
    13021 gttggccttt ttctttcaga ccagcatttt tcagttcgtc ggggatagtg gcatcttcta
    13081 accaacccat atcgccgccg ttacgggcag agataatatc ggcagatttt tctttggcta
    13141 atgcagcaaa atcaccgcct ttattcagct catcaagtac cgctttcgct tcatcttcag
    13201 ttttggtctg gatgatgctg tagcgggtac gctgcggctg ggtgaattga tcctgatgct
    13261 gatcgtagta gctctggata tccgcatcgc taaccggttg ctgcatcgtt gcggcaacca
    13321 gcttgatgta actcacgcgg aattgttccg gcgtcatgaa attgtttttg ttttgttcgt
    13381 agtagctggc aatctcctgt tcggtcacag gctgcttcgc cgccagcgcg ttaacatcga
    13441 tagtcgcctc acgcaccacg cgttgttgcg cgaccagtgc cgccagctcg tcggtttcac
    13501 ctttcagcat aaaatcggta ccggcaacgc cgttaatcag ctgttgggta gtgagctggt
    13561 tacgcagcgc ctgggcgtac tgatcggcgg tcatccccat ctggttgagg ataccgttat
    13621 agcggctgtt atcaaatttg ccgtcaacct ggaaggctgg ggtcgcgaaa atcgcctgtt
    13681 taacctgctc atcactgata cccagtttca gctcacgagc gtactgatcc aacagcgcct
    13741 cgtcgatcag acgattcagc acctgttgac gcagggtttt catatagcct tcgttcgctg
    13801 ccagctcgga gtattgatcg cccagctgtt gctgcatacg attacgctcg ctgttaaagg
    13861 cgttctcgaa ctgcccacgg ctgatttcct ggtcattcac ttttgcggcg tagttattgc
    13921 ctccgccaat caggtaacca ctcacgccgg tcaatatgaa cgacacgata atgataccga
    13981 aaataatctt gagcacgaga ctgtttgcag ccgtgcgtaa gctgtccatc atggtgtaac
    14041 aacactccgc tgtagatgac tgtgtacctc acgcaacgta tcgatgcgcg caaaggccta
    14101 ttgtgacaag aaacgggggc aattgtcagc ccacaagaca cagaaagtca ccgaatacaa
    14161 ataaaaaagg cacatcagta gatgcgccct tgaacttcgt cacatcccca ctggggacaa
    14221 cgcttagttt accgcgtctt tcagtgcttt acctgcacgg aagctcggta ctttagcggc
    14281 agcgatggtg atctctttac cggtctgcgg gttgcggcca gtacgggcag cacgctcttt
    14341 aacggcaaaa gtaccaaaac ctaccagtgc tacatcatcc ccttctttca gagattcagt
    14401 tacggaagca ataatagcat ctaacgcacg gccagccgca gctttagaga tatcagcccc
    14461 tgcagcaatc ttgtcgatca attgagattt attcactctt ctcttcctct ttataattta
    14521 tatcgcactt gaatccttca aggtacgaac gcgcagcagt tatatcaggc cagccatccc
    14581 cttacaacac cccttaataa gggcaagccc gatccgctat ctaacttagc gagacaaaaa
    14641 aaggctggca agcccgaatt agcctgccag ccctgttttt attagtgcat tttgcgcgag
    14701 gtcactattt tgcagtcaca acctgcatgc cagacggttc attttgcagc gccagagtca
    14761 gaacttcctc aatgcgcttc acaggatgaa tgtccagatc ggcaattacg ttgtcaggaa
    14821 tctcttccag atcgcgttta ttttcgaacg gaattagcac tgttttaatc ccgccgcgat
    14881 gcgctgccag gagtttttct ttcaaaccac cgatcggcag tacctgacca cgcagagtga
    14941 tctcaccggt cattgccaca tcggcacgaa ccgggttacc ggtcaggcaa gaaaccagcg
    15001 cggtgcacat agcaataccg gcactcggac catctttcgg cgtcgcacct tccggtacgt
    15061 ggacgtggat gtcacgtttt tcgtaaaaat cagggttgat ccccagtttt tccgcacgcg
    15121 cacgaaccac cgttaacgcc gcctgaatag actcctgcat cacttcgccg agcgaaccgg
    15181 tataggtcag tttgccttta cccggaacac atgcggtttc aatggtcagc aagtcaccgc
    15241 ccacttccgt ccacgccaga ccgattacct gaccgacacg gttttcgtta tcagcgcgac
    15301 catagtcgaa acgctgaaca ccgaggtagt catgcaggtt atcgccgtta atttcgatat
    15361 gttttaatga cttatcgagc agtaactgct taaccgcttt gcggcacagt ttggagattt
    15421 cacgctccag accacgcacg cccgcctcac gggtgtagta acgaataatg ccgataatgg
    15481 cgctatcgtc gacggtcagc tcaccttttt tcagtgcatt acgttcaatc tgcttcggca
    15541 gcaggtgacg tttggcgatg ttcagttttt catcttcggt ataaccggag aggcgaatca
    15601 cttccatacg atccagcagc ggtgccggaa tgttcatgga gttcgacgtc gcgacaaaca
    15661 tcacgtcgct gagatcgtaa tccacttcca ggtagtggtc gctgaacgct acgttctgct
    15721 ctggatccag cacttcaagc agtgcagagg ccggatcgcc tcgcatgtca gaagacattt
    15781 tgtcgatctc atcgagcagg aacagcggat ttttcacgcc cactttcgcc attttctgga
    15841 tcagtttacc cggcatagaa ccgatgtaag tacggcggtg accacggatt tccgcttcat
    15901 cacgcacgcc gcccagcgcc atacggacat atttacgccc ggtggctttg gcaatggact
    15961 gaccaagaga ggttttacct acccccggcg gccctaccag gcagaggatc ggtcccttga
    16021 ttttgttgac acggctttga accgcaaggt actcaaggat gcgatctttc acgcgctcca
    16081 gaccataatg gtcggtatca aggatttcct gcgcctgacg caggtctttt ttgaccttgc
    16141 tgcgcgcatt ccacggtacc tgtaccatcc agtcgatata accacgcact acggtcgctt
    16201 ctgccgacat cggagacatc attttcagct tctgcaactc tgcttccgct ttctcttttg
    16261 cctctttcgg catcttcgcc gcgtcgattt tgcgcttcag ggcttcgttt tcgtccggca
    16321 cgtcgtccat ttcaccgagt tctttctgaa tagctttcat ttgctcgttc agatagtact
    16381 cacgctggga tttctccatc tgctttttaa cgcggttgcg aatgcgtttc tcaacctgca
    16441 gcagatcgat ttccgattcc atcattgcca tcagatattc cagacgttcg ttaacgtcgg
    16501 acatctccag aacggactgt ttgtcagcca gtttcagcgg catatgtgca gcaatggtat
    16561 ccgccagacg cgccggatcg tcgatgctat tcagcgacgt cagcacttct ggtgggattt
    16621 ttttgttcag cttgatgtag ccttcgaact ggctgattgc agtacgcacc agcacttcct
    16681 gttcccgctc atcaatggtc ggcgactcca gatactccgc cttcgcagaa aagtgttcgc
    16741 cattgtcaga gagcgcagaa atacgcgcgc gctgtaaccc ctcgaccagc actttgacgg
    16801 tgccgtcagg cagtttcagc atctgcaata tagaggccac ggtcccgacg gtgaaaagat
    16861 cgtttacacc cggctcatcc gttgaagctt ctttctgcgc gaccagcata atttttttat
    16921 catggtccat cgccgcttcc agacaacgga tagatttttc ccgcccgaca aataagggga
    16981 tgaccatgtg cggataaacc accacatcgc gcagcggcaa tacggggatt tcaatgcgtt
    17041 cagaacgctc aggattcata gagctctctc ttagtttaat ttccgccagg taatcagatg
    17101 acacgactgt gcttcacgcc atctattaac atgtacgtca gtatatgggg atgtttcccc
    17161 cacattcaac gccgagaata gaggaaaaat taaaggggag ataaaatccc ccctttttgg
    17221 ttaactaatt gtatgggaat ggttaattat tcaccagatg cctgttgcgc ttccggctta
    17281 ccataaatca gcaacggttt gctttgacca tcaattaccg actcgtcgat aaccactttt
    17341 tcgacgtctt ccatggacgg cagatcgtac atggtatcga gcagtgcggc ttctacgatg
    17401 gaacgcaggc cacgggcacc ggttttacgc gccatcgctt tcttagcgat agcatccagc
    17461 gcctcgtcac ggaattccag atccacgcct tccagattaa acagcgcctg atactgcttg
    17521 gtcagggcgt ttttcggctc tttgaggatc tgaatcagag cttcttcgct cagttcattc
    17581 aacgttgcta caaccggcag acgaccaatg aactcaggga taagaccaaa cttgatcaga
    17641 tcttccggtt caacctgcgc cagcagctcg ccttcgcttg ctttgtcgga cttcgctttt
    17701 accgtcgcgc caaaaccaat gccggagccg gtttctacac ggtgggaaat cactttatcc
    17761 agaccggcaa acgcaccgcc acaaataaac aggatcttag aggtatcaac ctgcaagaat
    17821 tcctgctgcg gatgtttacg cccaccttgc ggtggaacag cagctaccgt accttcgatc
    17881 agtttcaaca gtgcctgctg tactccttca ccggaaacgt ctcgggtaat ggacgggttg
    17941 tctgacttac gagaaatctt gtcgatttca tcgatgtaga caatcccgcg ctgcgctttc
    18001 tgtacgtcgt aatcgcactt ttgcaacagc ttctgaatga tgttttcaac gtcttcgccc
    18061 acataaccgg cttcggtcag cgtggttgcg tcggccatgg tgaacgggac gtccagcagg
    18121 cgcgccagcg tttcagccag cagcgtttta ccggaaccgg tcggaccgat cagcagaatg
    18181 ttacttttgc ccaactcgac accattgctg gtatcgccgt tgcgcagacg tttgtaatgg
    18241 ttgtataccg cgaccgccag cacttttttc gcctgttcct ggccgataac gtaatcgtcc
    18301 aggtggttgc gaatttcatg cggcgtcggt agcgcactgc gttcacgatg cggtgcaact
    18361 tctttaatct cttcgcgaat gatgtcgtta cataaatcaa cacattcgtc gcagatatac
    18421 acggatggac cggcaatcag cttgcgcact tcatgctggc ttttgccgca aaaagagcaa
    18481 tacagcaatt tgcctgagcc atctttgcgt ttatctgtca tgggtcaaaa cctcttcttt
    18541 gttctttgtg ccgcacacga cgacgcaaat gccattctca ggcgcaagcc gcttacagcg
    18601 ttgtgccgcc ctagataagt atagcggcac agttgcgcct ctggcatcaa ttacgatggg
    18661 tcagaatcga atcgaccaga ccgtattcca ccgcttcagg ggcggaaagg aagcgatcgc
    18721 gctcggtatc acgttcaatc tgttctaatg attgacccgt atgaagcgcc ataagttcat
    18781 tcatgcgccc tttaactttc agaatttcac gggcatgaat ttcgatatcg gtcgcctggc
    18841 cctggtagcc gcccaacggt tggtgaatca tcacgcgcga attcggcagg caaaaacgtt
    18901 taccttttgc ccctgcggtc aacaagaaag cgcccatcga ggccgcctgg cccatacaga
    18961 tggtgctgac atcaggcttg ataaactgca tggtgtcata gatagacatc ccggcagtga
    19021 tcaccccgcc tggggagtta atgtacagat agatatcttt ttctgggttt tccgcttcca
    19081 ggaacagcat ctgcgccaca atcaggttag ccatgtggtc ttcaacctgg ccagtcagaa
    19141 aaatgacgcg ttccttaagt agacgagaat agatatcaaa agagcgctca ccgcgtgagg
    19201 tctgttcaat gaccatcggc accagcgcca tatggggtgc aaagttatct cgttcgccgc
    19261 tgtatgacat ttccgtctcc tggataaaat tgaaaaaaac ctgctgtacc gattgtaacc
    19321 ttatggacgg attatcagct agtccctgtg ttatgggtac ggcatcacca tatttcaagc
    19381 ataacaatct tttgttgtta cgctaacacc gaaagaggct tttcgcacgg ctccatgcaa
    19441 aattcatgac aaaaaaaagc ccaccacctg gcggtgacgg gcctttgtgc gaatttagcg
    19501 cgttatgctg cgtaaattac gcctgctggt tcatcagctc gttgaaagtg gtttcttttt
    19561 cagtcacttt cgctttcgcc agtacagctt caacagcctg ttcttccaga gcaacattgc
    19621 gcatgttgtc catcagttct ttgtttttgc tgtagaactc gataacttct ttcggatctt
    19681 cgtacgcaga agccatctct tcgatcaggc ctttcacgcg ctcttcgtca gctttcagct
    19741 cgttggtgcg gataacttcg cccagcagca ggccaacaac tacgcggcgt ttagcctgtt
    19801 cttcgaacag ttcgcgcggc agttccagag cttgtttttc gttgccaccg aaacgctgtg
    19861 cagcctggcg acgcagaacg tcgatttcgc tgtcgatcag cgcagccggt acgtcgatgt
    19921 cgttagcttt taccagacct tcgatcgcct gagacttaac gcggttacgg atggcgctct
    19981 tcagctcgcg ctccatgttt ttacgcactt cagcgcgcag accttctacg gaaccatctt
    20041 caacgccgaa acgtttgatg aattctgcag tcagttccgg cagttcacgc tcttcaactt
    20101 tcttcaggtt gatagcgaat ttcgctgctt tacctttcag gttttctgcg tggtattctt
    20161 ccgggaaggt cacgtcgatg gtgaactctt cgccagcttt gtggcctttg ataccgtctt
    20221 caaagcccgg gatcatacga ccctggccca tcgccagtac gaaatcagac gctttaccgc
    20281 cttcgaactc ttcgccgtct acagaaccgg tgaagtcgat ggttacgcgg tcttctgctt
    20341 caacagcgcc gtctttttct ttccaggtcg cctgctgttt acgcagagta tccagcatgc
    20401 cgtcaacgtc agcgtcggtc acttcaacga tcggtttttc aacttcgatc gcttccagac
    20461 cctgcagttc aacttccgga taaacttcaa actctacaga gtaagtgaag tcttcaccca
    20521 gcttgtattc gcccggaaca taagtcggtg cgccagccgg attgattttt tctttaatga
    20581 tggcgtcaat gaagttacgg ctcatcaggt cacccagaac gtcctggcgt acagacgcgc
    20641 cataacgctg agcaacgata ttcattggca ctttgccttt gcggaagccg tcaatacgta
    20701 cttttttcgc aacgttgacc agctcgcttt taacagcggt ctcgatgctg tcagcagcga
    20761 tagtaatcgt tacacggcgg ccaaggcctt gagtggtttc aactgaaact tgcatcttgt
    20821 tacctcaaaa aatcacagtg ctcggtcaac tcttaccccg caagcacagg ttgttggctt
    20881 cgcggaaccg ggatgttctt ataatcaatc acattccctg ttaccagaat catcccgaaa
    20941 gcattcaaat aggacgcggc attatagcgg catcactttt atgagtcgag aacggttatt
    21001 gcgcgctgtc gcgtattttt tcacaattcc cgggcaattt tcgtctgaat gctgggcaaa
    21061 aagcgcaaaa tccagacaaa acaaaacggc ccgcaggccg tttttcataa aatctttagc
    21121 aacatactgg aaaagcgccg acagttgcaa atgcgttttt acgcgatgct tcctgctcca
    21181 cgacagggag gagaggcaaa gacggtgtcc tgcaaccctt cccactcctt aatagtgtaa
    21241 gtatgcagag ccagcgcatg aacggtagta gagagttcct ccgctaaagt actgtaaatc
    21301 attcgatgac gattcagaaa acgttcaccc gtaaaacgat cgctgaccag cacaacttta
    21361 aaatggcttt cagagccggc tgggacattg tgacgatagc tttcatccac tacttcgagg
    21421 aatacgggtt ggaacgccgc ccttaatttt tcttctatcc gctcacgtat catcatgaaa
    21481 tatctcctcc gacaacgctg agatgtcatc catcccttta aatactagcc gcttttaccg
    21541 tttccattgc agcttaacaa caaatattta ggtttcgtcc tggtttttcc gtcaagcgta
    21601 tgaagtttac tgatttaaag attgaaacac aggcctcgca tcagcgtttc ttttaccagg
    21661 ggtgattatt tcgtcaacat gatgaattta catgccttac ccacttcccc tcgccgttgg
    21721 caatgttatg atggcgggaa tttttaccaa acacgcttaa gagtctctga gagcccgaac
    21781 atgttcaaaa aaatcctctt cccgttagtt gctctgttta tgcttgcagg atgcgcaaaa
    21841 ccgccaacaa ctattgaagt ttccccgacg attacactgc cacagcagga tccaagcctg
    21901 atgggcgtca ccgtaagcat taatggtgcc gatcagcgta ccgatcaggc gctggcaaaa
    21961 gtcacccgcg ataatcaaat cgttaccctg accgcctccc gcgctctgcg tttcctgctg
    22021 caagaagtgc tggaaaaaca gatgaccgcg cgtggttaca tggttggtcc gaatggcccg
    22081 gttaatctgc aaatcatcgt tagccaactg tatgctgacg tgtcccaggg caacgtgcgc
    22141 tacaacatcg ctaccaaagc ggatatcgcc atcatcgcta ccgcgcagaa tggcaacaaa
    22201 atgaccaaaa actatcgtgc cagctacaac gttgaaggtg cgttccaggc ctccaacaaa
    22261 aatatcgccg atgcggttaa cagtgtgctg accgatacca tcgctgatat gtctcaggac
    22321 accagcattc acgaattcat caagcagaac gcgcgttaat ttctgccctc tggcccggtg
    22381 caagccgggc ctgtagacgc ccatgtccag tcaatattta cgtatttttc aacagccgcg
    22441 ttcagccata ttgctgatcc tgggttttgc ttccgggcta ccgctcgccc tgacatccgg
    22501 caccttacag gcctggatga cggtcgaaaa tatcgatctc aaaaccattg gtttcttctc
    22561 tctggtaggc caggcttacg tttttaaatt cctctggtca ccgctgatgg accgctacac
    22621 gcccccattt tttgggcggc ggcgcggttg gctgctcgcc acgcaaatcc tgttattagt
    22681 cgccattgcg gcgatgggtt ttctcgaacc aggcacccaa ctccgctgga tggcggcgct
    22741 ggcggtggtg atcgcttttt gctctgcctc ccaggatatt gtcttcgatg cgtggaaaac
    22801 cgatgtgctc ccggcagaag aacgtggtgc gggcgcggca atcagcgtgc tgggttaccg
    22861 tttagggatg ctggtttccg gcggcctagc cctgtggctg gcagataaat ggttgggctg
    22921 gcagggcatg tactggttga tggcggcgct gttgatcccc tgtattatcg cgacgttgct
    22981 tgcaccagaa ccaaccgaca ccattcctgt gcccaaaacg ctggaacaag cggttgttgc
    23041 acctctgcga gatttctttg gtcgcaataa tgcctggctt attttgcttc ttatcgtgct
    23101 gtataagctg ggcgacgcat tcgccatgag cctgacaacc acgtttttga ttcgcggcgt
    23161 cgggtttgat gcgggtgaag taggcgtggt aaacaaaacg cttggcttac tggcgaccat
    23221 tgttggcgca ttgtacggtg ggattttgat gcagcgcctg tcgctgttcc gggcgctgct
    23281 gattttcggc attttacaag gtgcgtctaa cgctggttac tggctgctgt cgattactga
    23341 taagcatctc tacagcatgg gcgcagccgt ctttttcgaa aacctctgtg gcgggatggg
    23401 cacatcagcc tttgtcgcgc tgttaatgac gctgtgtaat aagtcatttt ccgctactca
    23461 atttgccctg ctctcagcgc tttccgctgt agggcgagtt tatgtcggcc ccgtggcggg
    23521 ttggtttgtt gaagcacacg gctggtcgac attctatcta ttctccgtcg ccgctgccgt
    23581 accggggctt attttgctgc tggtttgccg ccagacgctt gaatatacac gagtaaatga
    23641 caactttatc tcccgtaccg aatatccggc aggttatgcc tttgccatgt ggacactggc
    23701 ggcgggcgtc agcctgttgg ccgtgtggtt acttctgttg acgatggacg cgctggattt
    23761 gacgcacttc tctttcctgc ctgctctgct ggaagtcggg gttttagtcg ccctttctgg
    23821 cgtcgtgctt ggtggtttgc tggattatct ggcgctacga aaaacgcatc tgacgtaatc
    23881 tgtaaatatt atttagctgt agtaatcact cgccgtaatt attacggcta aataaacatc
    23941 agcatcgctt attaatattt gttcttttgt gcggcttagc gtttggaaat tattgacgcc
    24001 atttataaat tctatttttc ttacacgatt cagctaatga gtctttattt tctcaccacc
    24061 cagttgtcac tttaatgata attatttgtt aaataattgt tttatttcgc attggttata
    24121 ccaattgccc accaagactc cttctgcact ccccttttgt tata
//
LOCUS       DAJTYM010000060        24079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTYM010000060 DAJTYM010000000
VERSION     DAJTYM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..168
                     /gene="yjbD"
                     /locus_tag="OO575_002436"
     CDS             <1..168
                     /gene="yjbD"
                     /locus_tag="OO575_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6665364.1"
                     /translation="NSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFS
                     WSANTSTRGRR"
     gene            complement(301..1173)
                     /gene="rluF"
                     /locus_tag="OO575_002437"
     CDS             complement(301..1173)
                     /gene="rluF"
                     /locus_tag="OO575_002437"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6665365.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1521..2468
                     /locus_tag="OO575_002438"
     CDS             1521..2468
                     /locus_tag="OO575_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6665366.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2538..3344
                     /locus_tag="OO575_002439"
     CDS             2538..3344
                     /locus_tag="OO575_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6665367.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3354..3761
                     /locus_tag="OO575_002440"
     CDS             3354..3761
                     /locus_tag="OO575_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6665368.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3761..4255
                     /locus_tag="OO575_002441"
     CDS             3761..4255
                     /locus_tag="OO575_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6665369.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4321..5118
                     /locus_tag="OO575_002442"
     CDS             4321..5118
                     /locus_tag="OO575_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6665370.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5129..5917
                     /locus_tag="OO575_002443"
     CDS             5129..5917
                     /locus_tag="OO575_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6665371.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5969..7198
                     /gene="sorE"
                     /locus_tag="OO575_002444"
     CDS             5969..7198
                     /gene="sorE"
                     /locus_tag="OO575_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6665372.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7580..8269
                     /gene="pepE"
                     /locus_tag="OO575_002445"
     CDS             7580..8269
                     /gene="pepE"
                     /locus_tag="OO575_002445"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6665373.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8360..9991)
                     /locus_tag="OO575_002446"
     CDS             complement(8360..9991)
                     /locus_tag="OO575_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6665374.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10211..13894)
                     /gene="metH"
                     /locus_tag="OO575_002447"
     CDS             complement(10211..13894)
                     /gene="metH"
                     /locus_tag="OO575_002447"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6665375.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14094..14918
                     /gene="iclR"
                     /locus_tag="OO575_002448"
     CDS             14094..14918
                     /gene="iclR"
                     /locus_tag="OO575_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6665376.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15235..17419
                     /gene="espL4"
                     /locus_tag="OO575_002449"
                     /pseudo
     CDS             15235..17419
                     /gene="espL4"
                     /locus_tag="OO575_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17388..19125)
                     /gene="aceK"
                     /locus_tag="OO575_002450"
                     /pseudo
     CDS             complement(17388..19125)
                     /gene="aceK"
                     /locus_tag="OO575_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19309..20613)
                     /gene="aceA"
                     /locus_tag="OO575_002451"
     CDS             complement(19309..20613)
                     /gene="aceA"
                     /locus_tag="OO575_002451"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6665377.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20643..22244)
                     /gene="aceB"
                     /locus_tag="OO575_002452"
                     /pseudo
     CDS             complement(20643..22244)
                     /gene="aceB"
                     /locus_tag="OO575_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22513..23442)
                     /gene="metA"
                     /locus_tag="OO575_002453"
     CDS             complement(22513..23442)
                     /gene="metA"
                     /locus_tag="OO575_002453"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6665378.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23599..24042
                     /locus_tag="OO575_002454"
     CDS             23599..24042
                     /locus_tag="OO575_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6665379.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5942 a         6142 c         6106 g         5889 t
ORIGIN      
        1 aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag
       61 gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc ggatcctgaa
      121 gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg aacaatcaac
      181 gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa ccctctcccc
      241 gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct gaggcgttaa
      301 tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg
      361 accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc ccgctgtttt
      421 cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga gcttaaagag
      481 gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc cgcttaagct
      541 gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat gctcgcacat
      601 gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa acggcgcttc
      661 ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg cccccatacc
      721 acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt tctcatgatc
      781 gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga aaatcagccc
      841 ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc
      901 gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg gcttgttcag
      961 ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt ttactttcac
     1021 aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa ggaacacatt
     1081 gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta
     1141 tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac gcggaatata
     1201 ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta aatgtgatct
     1261 gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc ctggcaaaat
     1321 aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac tacgcagaga
     1381 aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa atcgctttga
     1441 aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt tgcacaaatg
     1501 tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt gtgaagattg
     1561 cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt
     1621 accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg gtcaccatcg
     1681 ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag caaaagtttg
     1741 gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact caactggcga
     1801 tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc gatattgtcg
     1861 gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat
     1921 cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc gaaagccgct
     1981 atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg
     2041 cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg cagtctcagc
     2101 actttaaaac catctctgcc tactgggata atctggatat cgccctggtg ggaattggct
     2161 caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa gagagtgacg
     2221 acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat attcacggcg
     2281 caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat aaattaaagc
     2341 aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga attattggtg
     2401 cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct gaactattac
     2461 tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc cctattatct
     2521 aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc attattgtca
     2581 ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca caaggcgcga
     2641 atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat aaaggctatc
     2701 agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta gcggaaatta
     2761 tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc
     2821 tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct gcattcgaaa
     2881 aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg gcgcgacaga
     2941 tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg ctggaaggct
     3001 cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc acgcgctcct
     3061 ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc gggattctgg
     3121 aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg cgcaatatca
     3181 ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg cgcgccggaa
     3241 gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc agctacatca
     3301 ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc tttatggtca
     3361 acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg gttcagatgg
     3421 tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac gccggagaca
     3481 ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg ctgattgccg
     3541 tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg cgcaatcctc
     3601 gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt gataaccagg
     3661 acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg
     3721 ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta cgctcgcccg
     3781 cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag tggctaacgc
     3841 ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc ggacattatt
     3901 gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc
     3961 ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca ctcgcccaca
     4021 ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata ttggcggcat
     4081 ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg atgatgatat
     4141 taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg tcgtagcgtc
     4201 agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa attaaataat
     4261 agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag gtgacaaatc
     4321 atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat tgccggaatg
     4381 ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac ggtgattggt
     4441 ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga attgatagct
     4501 ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc cagcataatc
     4561 tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg
     4621 cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac cgtagtgttc
     4681 cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga tattctgcat
     4741 gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat tgtctcactg
     4801 ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt tgtgacccga
     4861 ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt gctacgtatg
     4921 atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg tggctacctc
     4981 gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt ctacatccag
     5041 ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc caccgccctt
     5101 gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac gcagcgatct
     5161 ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg aacgtattca
     5221 cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc cattaaaaga
     5281 ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc cagccgtatg
     5341 tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca aagtcggtct
     5401 gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc gcccgattac
     5461 cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc tgctgttctt
     5521 ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc tcggctttcg
     5581 caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac tcaccgaagg
     5641 cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga cgtcaatcaa
     5701 cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca ccatgaccgt
     5761 gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga cgctactaat
     5821 ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc tgtttggctt
     5881 agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg cacgactgcc
     5941 gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc tttatggtaa
     6001 acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg aaatcctcgc
     6061 aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc tgggtgaaaa
     6121 ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc acgaattttg
     6181 cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg gtcagcgtta
     6241 tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct actccttccc
     6301 gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg aacaatattg
     6361 cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac cgctttcctg
     6421 cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata accacacgat
     6481 ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa tgggactgtt
     6541 ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca ttaccgatac
     6601 cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc aaacactgat
     6661 tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga gtggcggtca
     6721 cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc tcgcctcttc
     6781 cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata aacatttcag
     6841 cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg gcacgtcagg
     6901 cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa aagtgcaggc
     6961 cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca cgcttgaatt
     7021 gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc cgctgacgtc
     7081 actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc aggggatctg
     7141 gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc atgattaatc
     7201 gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg accggatgtg
     7261 cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc agcgccttaa
     7321 ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga atgaagcctt
     7381 attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag gccaggtaag
     7441 cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg caatacgtga
     7501 catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct tccgtgattt
     7561 cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc tgccgggtaa
     7621 agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc gccgctcagc
     7681 ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag agaaaacggc
     7741 tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg tcgatcccgt
     7801 tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt tccagttgct
     7861 gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta aacgtggcgc
     7921 gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc gtaccaccaa
     7981 cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc cgttgcaaat
     8041 caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc gtgagcagcg
     8101 tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc cggaaggtaa
     8161 ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca cttatgtgtt
     8221 taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag tgaattgccg
     8281 gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc gtcttatcag
     8341 gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc ggctgttcca
     8401 gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct cccagtaagc
     8461 ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg cgatcgacgt
     8521 gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga cgcaaacgac
     8581 gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa ttactgagca
     8641 attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc cgccgtgctg
     8701 ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag gcctgttcaa
     8761 ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc gccaactctt
     8821 cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg atatcatctg
     8881 ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta ttcagccctt
     8941 ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg ttcgccagag
     9001 caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc tgggtatcca
     9061 gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca acaaatggca
     9121 gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc agttccgctt
     9181 ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc aggataatca
     9241 ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct gcggcattgg
     9301 cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca atcaccagac
     9361 agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc agcagtacag
     9421 cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca tccagcagaa
     9481 tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg atcggcgtta
     9541 cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata ccgacgcggc
     9601 ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg ataaaaataa
     9661 gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc gccgtcccga
     9721 catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc tgagcgacga
     9781 acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta acgccgatcc
     9841 ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta cgcaaacgcg
     9901 cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc agcagggcga
     9961 cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat cgttatatgt
    10021 tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc tttactgtgc
    10081 cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta taagtgtttc
    10141 atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg aaaagtgaca
    10201 gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc tcaacatcgg
    10261 agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga atttgcgcca
    10321 ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat gcaccaggcc
    10381 acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc agcagctccc
    10441 agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc ggacggatgc
    10501 cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc gcatagcccc
    10561 agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct aaacggtcgg
    10621 caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag gcatcagcca
    10681 gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct gctttaccag
    10741 aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt ttttcggtct
    10801 gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca cggtagattt
    10861 caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc ggattcagcg
    10921 ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc cgctgcgcct
    10981 caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc gaccaggtca
    11041 taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct tccacttcct
    11101 gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat gcgaaatcgt
    11161 tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc ccgtgctgaa
    11221 tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc tgagtatcgg
    11281 aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac accgtagggc
    11341 cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc gcgccgccaa
    11401 tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta accatctcgt
    11461 ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact tctttggcga
    11521 tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg tagttgttac
    11581 attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct ttcacggtgg
    11641 cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata aacggttcga
    11701 gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc aggaacattt
    11761 tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg ccttcaatca
    11821 cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg ataaactcgg
    11881 ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag cgccactccg
    11941 cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg gcaagctcca
    12001 gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc gcgtcgcgca
    12061 gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc cccatatcca
    12121 tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc accggatcgt
    12181 tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg tgcggcagtt
    12241 cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg tgctcttcaa
    12301 taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc gggaagccaa
    12361 cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt gcgcgagtat
    12421 cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag cgacgcaaca
    12481 atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag ttaacaatgc
    12541 ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag gagtcgatca
    12601 taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa cgcaccatcg
    12661 ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc gcgccgtttt
    12721 ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg atcaggcgct
    12781 tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac aggctatcgt
    12841 cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt tccggcagtt
    12901 tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc ggcgtggtcc
    12961 cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt atctgttttg
    13021 ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg gcgttcgggt
    13081 gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg cgcagttcat
    13141 cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc aatgagttgt
    13201 aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg atggtgccgg
    13261 agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc gcaaagaccg
    13321 ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc gcaccacctt
    13381 ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa gtgatgttgc
    13441 ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt gggccaagaa
    13501 caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca gcacaggcgc
    13561 gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc tggtaatccg
    13621 ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg cccgcttcaa
    13681 agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc aggtcgttgt
    13741 tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg gcttcgttca
    13801 gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata cgttcattta
    13861 actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg cttaacccag
    13921 atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa aagcgaccac
    13981 cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa ctattgcatt
    14041 agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct gtcatggtcg
    14101 cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca gcgactggac
    14161 aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa tccaatggca
    14221 gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg acccaccgcc
    14281 tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg ggacactggg
    14341 caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt aatttgttag
    14401 caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg gtcaatatgg
    14461 cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag tgtacccacc
    14521 tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc ggtgcgggta
    14581 aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac cgcaaagggt
    14641 tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa gatctcgccc
    14701 aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg ctacgttgcc
    14761 ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct atttccggac
    14821 cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg attaaagcgg
    14881 cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg ggcagaggca
    14941 atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa tcgcattgac
    15001 gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca ttatttttaa
    15061 acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga aacttttacg
    15121 gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat attaaatttt
    15181 ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat ttaaatgatt
    15241 actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca gacaaatgaa
    15301 caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc tggcaaggag
    15361 ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt tcgtcatatc
    15421 tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc tgataagaaa
    15481 gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat tgctacaata
    15541 tttgaagaat acctaatgga aattttacct caacgctcag acactcttga taaacaagaa
    15601 ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact taatggtcaa
    15661 tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct gcacaatata
    15721 ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac tcttcagctg
    15781 aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg ttgatttcca
    15841 gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt atgaaagcat
    15901 attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta ctggtggata
    15961 accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt acccattacg
    16021 tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg agcgaaagca
    16081 aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat agcctcctga
    16141 aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca cttccgaaag
    16201 aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta tcaccagcaa
    16261 cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg atgattcagc
    16321 tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat agtattaacc
    16381 tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag actattttca
    16441 attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc atgcatattc
    16501 tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca cgtaaggata
    16561 aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca catcatttag
    16621 ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc catgttttat
    16681 accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc gcgttacctt
    16741 tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg aaggcaaagg
    16801 atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc gatatcgtga
    16861 aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca gcttccgata
    16921 ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt atggccatgc
    16981 agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact ctttttaata
    17041 cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt ctaatgaata
    17101 tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga cggtttatct
    17161 tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta tggatttttg
    17221 gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat ttggtcaccg
    17281 ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct ttggctttac
    17341 ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca aaaaagcatc
    17401 tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc ttccacatgc
    17461 ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc
    17521 atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg aaactcttcc
    17581 gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc gtcttccgga
    17641 tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa
    17701 tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag catgtcgcca
    17761 gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc aatggcgtcg
    17821 cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat ccgccgctcg
    17881 attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt ccgctgcttc
    17941 ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca caaagttttc
    18001 aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca gttgatagca
    18061 ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt tgatgacttt
    18121 gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac gaatacccgg
    18181 cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc ggtagctttc
    18241 ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg ttttacctgg
    18301 cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa ccataaaata
    18361 agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc aggtatcaat
    18421 aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg tgccggaagg
    18481 cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt cgttcgccac
    18541 ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat ggcgaatgat
    18601 gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt cgctgataac
    18661 gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca gcgggcgggg
    18721 aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct cgggagtaag
    18781 cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg caatctcgaa
    18841 gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta aaaatgccgc
    18901 gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac caacgtgatg
    18961 atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt cggcctgttc
    19021 gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag cgtcgaagcc
    19081 ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc gccctcatca
    19141 ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt cggcaacggt
    19201 tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc
    19261 ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt agaactgcga
    19321 ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa taatagtcgt
    19381 cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg tatagccatc
    19441 tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca taccttcgcc
    19501 ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga tacctgctag
    19561 ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc tggcaatagt
    19621 tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag ccagcagttt
    19681 gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca gatccggcgt
    19741 ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac ggctgatcgc
    19801 ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc cggtaataaa
    19861 ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag catcggtacg
    19921 tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg cgaccagttt
    19981 ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc atttcttcac
    20041 tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca tcgctttcat
    20101 cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa cgatcggcag
    20161 gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt ggatctgatc
    20221 ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt ttgccggata
    20281 gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta cctgccatcc
    20341 cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac cgccagtcag
    20401 tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac gccacatttt
    20461 cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta atttcaccac
    20521 atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc actctttctg
    20581 taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag ttatgtggtg
    20641 gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc atcggaagtg
    20701 gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa acgttcttcg
    20761 cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa caaggctttg
    20821 gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca gatcgaggta
    20881 cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac gcagccgttg
    20941 ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg cataccttct
    21001 tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg cgcgtcttgt
    21061 tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa taccgccatt
    21121 gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt attggcttcc
    21181 agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc atctttgctc
    21241 ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca ggttttaatc
    21301 agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg tctgtctggc
    21361 aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta atcccagcga
    21421 ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc catctggaac
    21481 acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg cagattaaag
    21541 cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta ggactgggtt
    21601 ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata gttgtggaag
    21661 aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca ggtgacatgt
    21721 ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt gggcttgagc
    21781 tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc atcgcgcagg
    21841 ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc gaaatcggcc
    21901 ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc taccgggcca
    21961 gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt ccaatcagca
    22021 tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc aatatcttgc
    22081 tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg cgtcaccagc
    22141 tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc gccatacggc
    22201 cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca gctcctcgtc
    22261 atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt cgttcagcac
    22321 aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta attaaaaata
    22381 cttgataact tattgataac aatatgaata aaatttattc acttgctgag gtgcgtttcg
    22441 gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt aacgatcgac
    22501 tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg gcgtgatctg
    22561 atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac gccagctcgc
    22621 tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat ccgggtctag
    22681 tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat attcgggatg
    22741 gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat ccccttcttc
    22801 cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg gaaagtcagc
    22861 atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa gcgcatgagg
    22921 atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt gcttaggaat
    22981 gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg tcgaggtgac
    23041 gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga catcattaaa
    23101 ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct gttcctgaat
    23161 atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc gcgattcacg
    23221 ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa gcaggcgcag
    23281 aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa ccttaagtgg
    23341 acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt cttcacgcaa
    23401 gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat tacctcacta
    23461 catacgctta tacgtttaga catccagata gctgaagata accagaaaat ttgccaatgt
    23521 cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag aatataagaa
    23581 tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga ggaaggggag
    23641 gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt atcagcagag
    23701 tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc gttgtgggtc
    23761 gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca gcatatggat
    23821 gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct ggtggagcat
    23881 gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga gcaggcggtt
    23941 gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga cgatttgggg
    24001 aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc tgccgtgcct
    24061 gcccaagctg gagaagacg
//
LOCUS       DAJTYM010000061        23933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTYM010000061 DAJTYM010000000
VERSION     DAJTYM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..386)
                     /locus_tag="OO575_002455"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO575_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO575_002456"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO575_002456"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6665380.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO575_002457"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO575_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6665381.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO575_002458"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO575_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6665382.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO575_002459"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO575_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6665383.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO575_002460"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO575_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6665384.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO575_002461"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO575_002461"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6665385.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO575_002462"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO575_002462"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6665386.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO575_002463"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO575_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6665387.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO575_002464"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO575_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6665388.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO575_002465"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO575_002465"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6665389.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO575_002466"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO575_002466"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6665390.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO575_002467"
     CDS             10748..11212
                     /locus_tag="OO575_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6665391.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OO575_002468"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OO575_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6665392.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OO575_002469"
     CDS             12236..13672
                     /locus_tag="OO575_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6665393.1"
                     /translation="MPLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO575_002470"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO575_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6665394.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO575_002471"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO575_002471"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6665395.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO575_002472"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO575_002472"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6665396.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OO575_002473"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OO575_002473"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6665397.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO575_002474"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO575_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR6665398.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OO575_002475"
     CDS             complement(20505..20798)
                     /locus_tag="OO575_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6665399.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OO575_002476"
     CDS             complement(20795..22084)
                     /locus_tag="OO575_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6665400.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OO575_002477"
     CDS             complement(22140..23078)
                     /locus_tag="OO575_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6665401.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OO575_002478"
     CDS             complement(23098..23862)
                     /locus_tag="OO575_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6665402.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5911 a         5805 c         6301 g         5916 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga actgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgcc
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cga
//
LOCUS       DAJTYM010000062        22639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTYM010000062 DAJTYM010000000
VERSION     DAJTYM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..539
                     /locus_tag="OO575_002479"
     CDS             <1..539
                     /locus_tag="OO575_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCR6665403.1"
                     /translation="QAADNNMEFANYGPFELLQARQLIESNIAEFAATQVTKQDIMKL
                     MAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALAAIVEKMWTQRSHNPYWKKLHEH
                     IDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHLENTKIMLFNETSDDFEFNADRY
                     LFAENPVVHLDTATSGSK"
     gene            884..1546
                     /locus_tag="OO575_002480"
     CDS             884..1546
                     /locus_tag="OO575_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6665404.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1550..1933
                     /gene="mzrA"
                     /locus_tag="OO575_002481"
     CDS             1550..1933
                     /gene="mzrA"
                     /locus_tag="OO575_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6665405.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2080..2434
                     /locus_tag="OO575_002482"
                     /pseudo
     CDS             2080..2434
                     /locus_tag="OO575_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2472..2777
                     /locus_tag="OO575_002483"
     CDS             2472..2777
                     /locus_tag="OO575_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6665406.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            2780..3184
                     /locus_tag="OO575_002484"
     CDS             2780..3184
                     /locus_tag="OO575_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6665407.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3174..3473
                     /locus_tag="OO575_002485"
     CDS             3174..3473
                     /locus_tag="OO575_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6665408.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3659..3955
                     /locus_tag="OO575_002486"
     CDS             3659..3955
                     /locus_tag="OO575_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6665409.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4025..5011
                     /gene="yqjG"
                     /locus_tag="OO575_002487"
     CDS             4025..5011
                     /gene="yqjG"
                     /locus_tag="OO575_002487"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6665410.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5304..5672
                     /locus_tag="OO575_002488"
     CDS             5304..5672
                     /locus_tag="OO575_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6665411.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(5879..6775)
                     /gene="yhaJ"
                     /locus_tag="OO575_002489"
     CDS             complement(5879..6775)
                     /gene="yhaJ"
                     /locus_tag="OO575_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6665412.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            6880..7581
                     /locus_tag="OO575_002490"
     CDS             6880..7581
                     /locus_tag="OO575_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6665413.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7604..7768
                     /locus_tag="OO575_002491"
     CDS             7604..7768
                     /locus_tag="OO575_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665414.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(7980..9290)
                     /locus_tag="OO575_002492"
     CDS             complement(7980..9290)
                     /locus_tag="OO575_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6665415.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9318..10649)
                     /locus_tag="OO575_002493"
     CDS             complement(9318..10649)
                     /locus_tag="OO575_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6665416.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(10924..12288)
                     /gene="tdcG"
                     /locus_tag="OO575_002494"
     CDS             complement(10924..12288)
                     /gene="tdcG"
                     /locus_tag="OO575_002494"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6665417.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12360..12749)
                     /locus_tag="OO575_002495"
     CDS             complement(12360..12749)
                     /locus_tag="OO575_002495"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6665418.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(12763..15057)
                     /gene="tdcE"
                     /locus_tag="OO575_002496"
     CDS             complement(12763..15057)
                     /gene="tdcE"
                     /locus_tag="OO575_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6665419.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15091..16299)
                     /gene="tdcD"
                     /locus_tag="OO575_002497"
     CDS             complement(15091..16299)
                     /gene="tdcD"
                     /locus_tag="OO575_002497"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6665420.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16325..17656)
                     /gene="tdcC"
                     /locus_tag="OO575_002498"
     CDS             complement(16325..17656)
                     /gene="tdcC"
                     /locus_tag="OO575_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6665421.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(17678..18667)
                     /gene="tdcB"
                     /locus_tag="OO575_002499"
     CDS             complement(17678..18667)
                     /gene="tdcB"
                     /locus_tag="OO575_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6665422.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(18766..19704)
                     /gene="tdcA"
                     /locus_tag="OO575_002500"
     CDS             complement(18766..19704)
                     /gene="tdcA"
                     /locus_tag="OO575_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6665423.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            19894..20238
                     /gene="tdcR"
                     /locus_tag="OO575_002501"
     CDS             19894..20238
                     /gene="tdcR"
                     /locus_tag="OO575_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6665424.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20494..21034
                     /locus_tag="OO575_002502"
                     /pseudo
     CDS             20494..21034
                     /locus_tag="OO575_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21056..21403
                     /locus_tag="OO575_002503"
     CDS             21056..21403
                     /locus_tag="OO575_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665425.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21572..22222
                     /locus_tag="OO575_002504"
     CDS             21572..22222
                     /locus_tag="OO575_002504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665426.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNYLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6107 a         5715 c         5212 g         5605 t
ORIGIN      
        1 agcaggcggc tgacaataat atggaattcg ccaattacgg tccgtttgag ttgcttcagg
       61 ctcgccagct catcgaaagt aatattgccg agttcgcggc aactcaggta acgaaacaag
      121 acatcatgaa actgatggcc attcaggaac aggcgcgcgg cgaacaatgc ttccgtgatt
      181 ccgagtggga tttacagttc cacattcagg tagccctggc gacgcagaac tccgccctgg
      241 cggccatcgt tgaaaaaatg tggacccagc gtagccataa cccgtactgg aaaaaactac
      301 acgaacacat tgattcccgt accgttgata actggtgtga tgaccacgat caaattctca
      361 aggcgttaat tcgcaaagat cctcatgctg ctaagctggc aatgtggcag catctggaaa
      421 acaccaaaat catgttattt aacgaaacca gcgacgattt cgagttcaat gccgaccgct
      481 atctgttcgc cgaaaacccg gtagtgcatc tcgatacggc caccagcggc agcaaatgaa
      541 attcctgtcc gacaggcgct gcaatgcgcc tgtttgtctc gtcggtaagc gaaaagtata
      601 aagtgtcagc ctgtgtaaat cctctcgcca ccctcccctg caatcagcaa aatcagactc
      661 cacggacatg taattttgat aacgaacaac gttgaccttt gttacaatta gattcaattt
      721 gaatttatgc ttttgaatgc tttcttatct cacgttttaa cagggaatag ttcaggctgt
      781 gttgatgtat caaacccgca gaacatacca aaacagcaat aacattgcgg tagtgcatct
      841 tttaaaacca gcgtggcgtt aaccgattca ccaggaataa tgaatggaac ttttgaccca
      901 attgctgcaa gccctgtggg cgcaggattt tgaaaccctg gccaatccat cgatgattgg
      961 catgttgtat tttgtcttgt ttgtaatttt gttccttgaa aatggcttgc ttccggcggc
     1021 ctttttaccg ggcgacagtt tactggtatt ggtcggcgtg ttgattgcga aaggcgcgat
     1081 gggttacccg caaacgattc tgctgctgac cgttgccgcc agcctcggtt gctgggtcag
     1141 ctatattcag gggcgatggc tgggcaatac ccgcaccgta caaaactggc tatctcattt
     1201 acccgcgcat tatcatcaac gcgcacacca tctttttcat aaacacggtt tatcggcgct
     1261 gttaattggt cgctttattg catttgtcag aacactgctg ccgacgattg ccgggttatc
     1321 agggctgaat aacgcgcgct ttcagttctt caactggatg agcggtctgc tgtgggtatt
     1381 gatcctgaca actctgggtt acatgctcgg caaaacgccg gtatttttaa agtacgagga
     1441 ccagctgatg tcatgcctga tgctgctccc ggtggtgctg ctggtgtttg gcctggcagg
     1501 ttctctggtc gtgttatgga aaaagaaata tggaaatcgg gggtaaggga tgcaaatacc
     1561 tcgcatgtcg cttcgccagc tagcctggtc cggcactgtt ttacttctgg tcggcacgct
     1621 gttgctggcc tggtccgcgg ttcgccagca agagtctacg ctggcgattc gtgccgttca
     1681 tcaaggcaca acgatgccag acggtttttc gatctggcat caccttgacg ctcatggcat
     1741 tcctttcaaa agtatcaccc ccaaaaacga cactctgtta attacatttg actccagcga
     1801 ccagagcgcc gccgcaaaag cggtcctcga cagaacattg ccccatggct acatcattgc
     1861 gcagcaggac aataacagtc aggctatgca gtggctgacc cggttacggg ataactctca
     1921 tcgcttcgga taacttcctg ttatccgaaa catatcactc actttggtga tttcaccgta
     1981 actgtctatg attaatgagg cggtggttaa cactaccgct tcgttgtctg gacaatcgtt
     2041 cctttgtaat aggtccgaat cacaatggaa ggttcaagaa tgaaataccg catcgcttta
     2101 gctgtttctc tctttgctct tagtgccggt agttatgcca ctaccctgtg tcaggaaaag
     2161 gagcaaaata tccttaagga gatcagctat gccgaaaaac accaaaacca gaatcgtatt
     2221 gacggtctga ataaagccct gagtgaagtc cgggccaact gttcagatag ccagctgcgt
     2281 gccgatcatc acaagaaaat cgcaaagcag aaagatgagg tggcggaacg ccagcaagat
     2341 ttagccgagg cgaagcaaaa aggcgatgcc gatagaacgg aaactggcag aagcgcagga
     2401 agagctgaaa aagctggaag cgcgcgacta ctaactcaca atagtcacta cttactcacc
     2461 tggagaaaac tatgtcgaaa gaacacacta cggaacatct gcgtgctgag ttgaaatccc
     2521 tttccgatac gctggaagag gtgcttagct catctggcga gaagtcgaaa gaagagttga
     2581 gtaagattcg tagcaaagcg gagcaggcac tgaaacagag ccgttatcgc ctgggtgaaa
     2641 ccggtgatgc catcgccaaa caaacccgtg tcgcggcggc gcgtgctgat gagtatgtgc
     2701 gtgaaaatcc gtggaccggc gtgggaattg gcgctgcaat cggtgtagtg ctcggcgttc
     2761 tgctgtcgcg tcgttaatta tggcggacac tcatcacgcg caagggcccg gtaaaagcgt
     2821 tctgggcatc gggcagcgaa ttgtttccat catggttgaa atggtggaaa cacgtctgcg
     2881 gctggcggtg gtggagctgg aagaggaaaa agctaatctc tttcaacttt tactgatgct
     2941 gggcctgacg atgcttttcg ctgcatttgg tcttatgagc ctgatggtgc taattatttg
     3001 ggcggttgat ccgcaatatc gcctgaatgc gatgattgcc accaccgtgg tgttgctgct
     3061 actggcactg attggcggta tctggacgtt acgtaaatcg cgtaaatcca ccttgctgcg
     3121 ccatacacgc catgagctgg caaacgatcg gcaactgctc gaggaggagt cccgtgagca
     3181 gtaaagtcga acgtgaacga cgtaaggcgc aactgcttag ccagatccag caacaacggc
     3241 tggatatttc cgccagtagt cgtgaatggc tggaggcaac aggcgcttac gatcgtcgct
     3301 ggaatatgct gctaagtctg cgctcctggg cgctggttgg cagtagagtg atggcgatct
     3361 ggacgattcg ccatcctaat atgctggtcc gctgggccag acgcggtttt ggcgtatgga
     3421 gcgcctggcg tctggttaaa acgaccctca agcagcaaca gcttcgcggt taacttccct
     3481 ctggccggag caattccggc cttatccatc aaattttttg aatatttttg acagttttcc
     3541 ttgctaacaa tcatcattca ccacgtttat gattctctcc atcgacagca acgacgctaa
     3601 tagcgcgccc ttgcacaaaa aaacaatcag cagcctgagt ggcgcagtgg agagtatgat
     3661 gaaaaaatta gaagatgttg gtgtactggt agcgcacatt ttaatgccga ttctgtttat
     3721 taccgcaggc tggggaaaaa tcaccgctta ctctggtacc caacaatata tggaagcaat
     3781 gggtgtcccg ggttttatgc tgctgacggc atttttattt cacagcaact ttgctgaagg
     3841 cgtcaactcg ctgatgttca tgaaaaacct gacaatttct ggcggattcc tgctgctggc
     3901 aattaccggt ccgggcgcgt atagcatcgg ccgcctgctg aataaaaagt ggtaagcacg
     3961 cgctatactt aacgataaaa aacgaggagg aagctcctcg tttttgctat tggaggagag
     4021 aaaaatgggt caactgattg acggcgtctg gcatgacacc tggtacgata ccaaatctac
     4081 cggcggtaaa tttcaacgtt cagcttccgc atttcgtaac tggctcactg ccgatggcgc
     4141 acctggcccc actggcacag gcggttttat cgcagagaaa gatcgttatc atctctatgt
     4201 ttcactcgcc tgcccgtggg cgcaccgcac gctgatcatg cgcaaactca aaggactgga
     4261 accgtttatt tccgtttccg tagtgaaccc gctgatgctg gaaaacggct ggacctttga
     4321 tgacagtttt ccgggagcaa ccggagatac gctctatcaa catgaatttc tgtatcagct
     4381 ttatctccac gccgatccac actacagcgg acgagttact gttcccgtgc tgtgggacaa
     4441 aaagaaccac accatcgtca gcaacgaatc agcagaaatc atccgcatgt tcaataccgc
     4501 gtttgatgcg ctgggcgcaa aagcgggtga ttactaccca ccagccctgc aaacgaaaat
     4561 tgacgaactt aacggctgga tttatgacac cgttaacaac ggcgtgtata aagctggttt
     4621 tgccaccagc cagcaagcct acgacgaggc ggtggcgaaa gtgtttgaat cgctggcgcg
     4681 actggagcag attttaggcc agcatcgtta cctgaccggc aaccagctaa ccgaagccga
     4741 tattcgcctg tggactacgc tggtgcgttt tgatccagtg tatgtgaccc acttcaagtg
     4801 tgataagcac cgcatcagcg attacctgaa tctgtatggc ttcctgcgcg atatctacca
     4861 gatgccgggg atcgccgaaa ctgtcaattt cgatcacatc cgtaatcatt acttccgcag
     4921 ccataagacc atcaacccta cggggattat ttcaattggt ccgtggcagg atctcgatga
     4981 accgcatgga cgagatgttc gcttcggtta aaaaaaggca ccttacgagg tgcctctgat
     5041 aattagctaa gtgcatgttg ttcatgtcgg atgcggcgtg aacgccttat caaacctaca
     5101 aagcgtttat attcaatatg ttacaatgac cgcgtaggcc agataagcgt agcgcatctg
     5161 gcgattttgc gcttgccatc agtcttagcg cgccttcacg gcactttatt aattccttga
     5221 acgaatattt actgccattt cattcacgtc tattcttaat ttgctgcttt aaaaacaagt
     5281 gattgagcaa aattgaggca aaaatggact ggtatctgaa agtactaaaa aattatgtcg
     5341 gtttccgggg tcgtgcgcgg cgcaaagagt actggatgtt tattctggtc aacatcatct
     5401 ttacgttcgt gctggggctg ctggataaaa tgttaggctg gcaacgcgct ggtggcgaag
     5461 gcatcctgac gataatctac ggtattctgg tgtttttacc gtggtgggcg gttcagttcc
     5521 gccgcctgca cgacaccgac cgctcggcgt ggtgggcact gctattctta atcccgttta
     5581 tcggctggct tattatcatc gtctttaact gccaggcggg tacgccaggc gaaaaccgct
     5641 ttggaccaga tccaaaactg gaacaagaat aatgattaag caccttgtaa aaggtgcttc
     5701 aaattgttga caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccagcc
     5761 tacaaattgc caaaattcaa tatattgcag tgactgcgta ggcctgataa gcgtagcgca
     5821 tcaggcagtt ttgcgtttgt cagcagtcag aagcaccttg tgaaaggtgc ttaatagatt
     5881 attttccagc gaacaacttt ggaatttccc gcagacacca ggatttcgct tcccccatac
     5941 tgtcacgacg ccaggccata ataatatcga tctcgctggt cgattccggg ctgacgacac
     6001 gcaaccgccc ttccgcaata tctttttcga ccatcggata cggcatcgtc gccacgccaa
     6061 gccccgccag taatgcctga cgtttatctt caatcgtgct caccgttaag cgcggctgtt
     6121 tgtccagcag ctgtacggtc aacaccgggc gctcacgagc ggtatccgct accgcaattc
     6181 cacgatattt cacacgcgtc acttcagata acggttccgg ctcctgatga atcgggtgat
     6241 caggcgcggc aacgtagacg ttcattaacg tatagagctt gcgcgagttg atctccgacg
     6301 aggaacgaaa atgcatatcc ggcgcgataa cgatatccgc ccgcccctgc tccagccgtt
     6361 cccacgcccc cgccagcact tctgtgatga ttgccagttg ggtattggct tttgccgcca
     6421 gtttgtcgat taacgggaaa aaggcaggtg tcggtaccag cgcttcggtc acaatggtga
     6481 gatgcgtttc ccaaccgcgc gcgagagctt ccgcatcagt agtcagttta tctgcggctt
     6541 ccagcaaaac gcgccctcgc tccagcaaca tcctcccgac attggtgaat ttggtgcgat
     6601 ggcccgagcg gtcaaacagc accacatcca gctcttcttc cagtttttgc atggtgtagc
     6661 taagtgcgga aggcacgcgt cccagctcat ccgctgccgc cgcaaaactg ccccggcgat
     6721 cgatcgcatc cataacccgt agtgcttcca gcgttaatgc cctttctttg gccatttcgt
     6781 tctcattcag gaaatttgaa cataccgggc agaatatctg gctaacaatg tagcgtccag
     6841 ccccttacca taaaaggaag taaagagagg tcaagaatta tgattactac ccgaactgcc
     6901 aggcagtgtg gacaagcaga ctacggatgg ttgcaggccc ggtatacttt ttcctttgga
     6961 cactacttcg acccgaaatt gttaggctat gcctccctgc gtgtgcttaa ccaggaagtg
     7021 ctggccccag gtgccgcctt tcagccgcga acctatccca aagtcgatat tttaaatgtg
     7081 attctggatg gagaagcaga gtatcgcgac agcgaaggca atcatgttca ggccagcgcc
     7141 ggagaggcgt tgctgctctc tacccagccg ggtgtcagct atagcgaaca caatctcagc
     7201 aaagacaaac cgttaacgcg aatgcagctt tggctggata cctgcccgca gcgagagaat
     7261 ccgctgattc aaaagctggc gcttaatatg gacaagcagc aattaatcgc ctcgccagag
     7321 ggtgcgatgg gaagcctgca attacgccaa caagtgtggc tgcaccatat cgtgctcgac
     7381 aaaggcgaaa gtgcgaattt ccagttgcat gggccacgcg cgtatttgca atcgattcac
     7441 gggaaatttc atgcgcttac gcatcatgaa gagaaagcgg cgctaacctg cggtgatggg
     7501 gcgtttattc gtgacgaggc taacattacg ctggttgccg attccccact gcgcgctttg
     7561 ctgatagatt tgcctgtcta gttgttttta cagggagatg atgatgagta aaaaatcgac
     7621 caaaaagcgc cagccagcga agcccgtggt ggcgaaagaa cctgctcgca ccgccaaaaa
     7681 ttttggctat gaagagatgt tgagcgagct ggaagctatc gtcgcggatg ctgaaacgcg
     7741 tttagccgag gatgaagcta ccgcgtaaag acaggttgcc ggatgcggcg taaacgcctt
     7801 atccggccta caaaacacaa caacatcaac aaattaaaca ccccgtaggc cggataagat
     7861 gcgccagcat cgcatccggc atcaggtaca aaacacaaca acatcaacaa gttgagcacc
     7921 ccgtaggccg gataaggtgc gccagcatcg catccggcac aatactcaaa acctggcgtt
     7981 tatctggcct tgctcgccat aatctcgata atctgccgat ccgtttgctg catcgaatgg
     8041 cttgctaacg cacacaggtt ggcaatcgac tgctcaacat catgcgccac gatcccttca
     8101 ttgccggtca cggcggtatc atccagcgcc attaacaccg ctttccacgc agccgaagca
     8161 ctggtcgaaa ccttcatcgc gcagctgttc gacgcaccat cgcaaatcat gccgctgaca
     8221 tcgccgatca tactgctgat cgccatcgag atagtttcat aacgcccatc caccagccat
     8281 gccatcccgg cggcggcccc cattgctgcg gtcgttgcgg cacaaagtgc agacaaacgc
     8341 ggtaactggt tatggatgta aattgcgctc aaatgagaaa gcatcagcgc acgcgccagt
     8401 cgttcatcat ccgctccgaa gtgttctgct acaaccacca caggcattat tgcagtgatc
     8461 ccctggttac ccgagccgga gttactcata gccggaagcg tagcgccgcc catacgcgca
     8521 tcggatgccg cgctggtacg aatcacaatg gatgaagaga gatctttcgc cagcaaaccg
     8581 cgctcgcact gtttttccag cgtcgcgcca atatgcagcc cccacttacc gctcaaacct
     8641 tcctgcgata acgcacaatt tagcttcgcg gaatcgagaa taaagcggat cgccgcaaat
     8701 gggacttcat tgacgaactt caggatctca gccagcgtcg ttctggaaag caccgtcagc
     8761 ggcgactctt gctcgccctc tgccacacac gcctggtggg taaacaccac accattgtgc
     8821 gtctcaatat gcacaatgtt ggtatgcccg ccgacgatgg tgacacacgc ccacttctca
     8881 ccgttccaga ctttggcgcg tgagaagagg atttcattgc aaggttcctg gatcttaaca
     8941 gagactttcc ccgccgccag cagtgctttg gcatcggtaa ttgcctgcgc agttgcgtct
     9001 ttcagcactt ccagcccggc gttggcattt ccacctaacg cccccagcgc cgccgcaatc
     9061 ggcagcccca ccattcccgt gccgggaacg gtgacgccca gaccgttctt catcagattt
     9121 ggcgaaaccc aggcttctac acgttcaacc ggaccttcca gttctgccgc agcaaccgcc
     9181 gccgccagcg ccagtgaaat cggttcagta catcccagcg ccggttttac ttcctcctga
     9241 acggcgagga tgtaacgctg ccataacgga tttaaagtcg aatcaaacat attaaaaacc
     9301 ttaaaatttc aggtaaatca agaaaatgcc aggaacagag aaacacaaag caacaaacca
     9361 gtgacgataa tcaggtacag agacatccct ttgtatttgt gcaatgccgg tactttgtaa
     9421 accagccacg ccgggatcag gcaccctacc atgccgaaaa tcgggctaca gatagaggtg
     9481 aagctcaaca ccggtgcgtt cagtacgatg gcgctccagg ccagcaaaat ggcgaaaatc
     9541 atgatgccgc gctgaacaag attttcgtta atcttctcgg caggcatctt gcgacgcagg
     9601 atgttcatta cgatcccttg cgttgcttcg cgaaagccta aatagacgcc aaagaatgca
     9661 gtcatgacag caaagatatt gagaatgacg ctgaccactt tcacccacgc tgcgccgtca
     9721 ccgctaataa actgtgcggc aatcgccagc gcggaaatat tctgctcgta ggcttttacc
     9781 gcttcgtcat gtcccatcgc cagcgtgaac gacacggcgt agaaaaagac ggtgacaaac
     9841 aaaatgccaa acgcgatatt cattgcccgc aatgctttat gccgcgccac ttcaattgat
     9901 ttttcccgcg agcgataaga gatcaccatc gggcttaacg tctggataaa cagaatcgac
     9961 gtcagggtaa acggcagcgt aataatggcg tttttcacca gcagccccag cggcggtagc
    10021 gaaccgacgt tatacagatg ccacattccc accatcgaca cgcccagcgc agcgactacc
    10081 agcagcttgg tcagcaccat gtcggtcgaa attttgaata acaatttctc gccgcgtgaa
    10141 gagatcgcca ccagaatgca aatcagtacc agaccataaa agggactgtc tgacagcaac
    10201 ccttccgtca cgccgaaggt atgcagatag gaagcactat cgttggtgat ggcggtggaa
    10261 tagacgaaca tccaaatcac cagcattacg aaatagagcg cacctaacag gatgccccag
    10321 tttttaccta aataaccgct aatgacactc gggtaatctt tacattctgg tgattctgcc
    10381 agcgtattaa taaacaaccg ctgaaacaga tacattgccg ggtaaccaat caccgatgag
    10441 agcaaaaata cccacaatcc catcaaaccg acctgcaccg ggagaaaaac aatccccgcg
    10501 ccaatcgcca tcccgatact cataatcacc cagccggtgt cagtactgtc gaatttgatc
    10561 gcttctcgcc actcgctctc actcattcca gcacgacccg ccggggtcga agcgtctgca
    10621 atgacgcctt tattcgatgc aatttccata atttctcgct caatattttg tagggcttat
    10681 tatttttttc cgagccgcat caaggcgata tgcggtctac gtttttgcag gcgagttatt
    10741 agaataaaga aatgatacgc gcgaggtcag agaaaatctt cacaatcaag actttaaaat
    10801 aacggagatg gataagaatt ttctactaaa ttaatcacaa gagaatattc attctctatt
    10861 tgcggcagat cacaaaaaaa aggtgcacat ttgtgcaccc aaggatgaaa gctgacagca
    10921 atgtcagccg cagaccactt taatggccag tcctccgcgt gatgtttcgc ggtatttatc
    10981 gttcatatct ttgccggttt catacatcgt ctcgatcact ttatcgagtg aaacacgcgg
    11041 tgccgaggtg cggcgcatcg ccatccgcgc ggcgtttact gctttcacgg cattaatggc
    11101 attacgttca atgcacggga tttgtacctg tccggcaacc ggatcgcagg tcagcccaag
    11161 gttatgctcc atcgcgattt ccgccgcatt gcatacctgc gccggactgc cgcccagtag
    11221 ttcagttaac cctgccgctg ccattgaaca ggccacgcca atctccccct gacagccgac
    11281 ctccgcgcca gagatggagg cgttcatttt atacagcgcg ccaatagccc ccgctgccag
    11341 aaaatagcgg gcaattgacc gctcgtttac cggacgacgg aacttatcgt aataagccag
    11401 cactgccgga ataatgccgc acgcaccgtt agtcggtgcc gttaccacgc gcccgccagc
    11461 tgcgttttct tcactaaccg ccagcgcgta catgttgatc cagtcgatga cattcatcgg
    11521 atcgttagag atgttatcgc tagaaaccag ctgacgacgc agcgctacgg cacgacacgg
    11581 cacattgagc ggaccaggca gcacgccttc agtgttcatc ccgcgttcaa taccgtcatg
    11641 catcacttgc cagatacggg caaaccctgc gtcaatttcc gctttgctgc gcagcgccag
    11701 ctcgttgtgc atcatcaggc cagatataga caggccgttg taatcacaca ttttcagcag
    11761 ttcacctgct gagtggaaat cgtaaggtac ggacgtttcg acatcgtgcg acaggccgaa
    11821 gtgttcttct tcgacaataa acccgccgcc gacagagtaa taggttttac ttaatagcgc
    11881 ttcctgtccc ttccaggcag tgatccgcat tccgttctca tggcgaggca acatttcggg
    11941 atggaagata atgttctttg ctacaggaaa atcaacaata tgcgcaccag atgccactgg
    12001 cagccgcccg ctgcgcgtta ctaactctat aaatgcaggg atctcatcaa tgacaacatc
    12061 ctgcggactg tttcctgcca gtcccatgat aatggcgaca tccgtggcat ggcctttgcc
    12121 cgtcagtgac aacgacccgt acagatcgac cacaatatgg ctcgtcgcgg ttaataagcc
    12181 gctactttcc agccgatcaa taaaactttt tccggcattc attggcccca cggtatgcga
    12241 actggaggga ccaatcccaa ttttgaaaat atcgaatgca ctaatcatat ccacaccctc
    12301 ggattgccgt tcagtgaagt ggagcggaac gaccttgcga ccgtcccgct cacgaggctt
    12361 tacgcactac gtactgcgat agcttcaatt tccagcttca catctttcgg caaacgcgca
    12421 acctgcacac agctccgggt cgggtaggtc gcctgatgct catcgaagaa ctgcttatag
    12481 acttcattga tggtggcaaa atcattcaga tcggtgatga acacggtcat cttgatgata
    12541 tcgcccacgc tcagcccggc agcaaccacg atcgctttga cgttttcgag gcttaaacgc
    12601 gcctgatctt gcacatcagc cgggatctca ccggtctgcg ggcaaaccgg aatttgcccg
    12661 gaggtgaaga ccatgctgcc taaatcaacg ccctgaacat aagggccgat tgcgcctggg
    12721 gcacgttgcg tttcgataat ctttttcata catcctccgg cgtcagagcg cctgggtaaa
    12781 agtacgtgaa ataacatcct gttgctgttc acgggtcagt gcgttgaagc gcacggcgta
    12841 gccagagaca cggattgtca ggttaggata tttttccggg tgctcgatgg catccagcag
    12901 catttcccga ttcattacgt tgacgttgag gtgttgaccg ccttcgacat ccgcttcgtg
    12961 gtggaaatac ccatccagca ggccgacaag gttggtttta cgtactggat cttctttgcc
    13021 cagcgccgca ggaacgattg agaaggtgta cgagatccca tctttggcgt aggtgaacgg
    13081 cagtttcgcc accgacgtca atgaggccac ggcacctttg cggtcacgac catgcatcgg
    13141 gttagcgccc ggcgcgaacg gtgttccggc gcgacgaccg tccggcgtat taccggtttt
    13201 ctggccgtac accacgttag aagtgatagt cagaatcgac tgggtaggga cggcgttgcg
    13261 ataggttggc agcgctttaa ttttcttcat aaagcgttca accaggtcgc aggcaatgct
    13321 gtctacgcgc tcgtcgttgt tgccgtactg cggatattca ccgtcgattt caaagtccac
    13381 cgccaggccg ttttcgtcac ggattggttt cacgcgggca tatttgatgg cagacaggga
    13441 gtccgtcgcc accgacaggc ccgcgatgcc gcatgccata gtgcgataga catcacgatc
    13501 gtgcagcgcc atcagcgaag cttcgtagct gtacttgtcg tgcatgtagt gaatgatatt
    13561 cagcgcgctg atgtactgca ccgccagcca gtccatgaag tgatcgaggc tgtccatcac
    13621 tttgtcgtaa tccagcacgt cgtccatcag cggtgctgtt ttcggcccga cctgaatctt
    13681 cagcttctcg tccaccccgc cgttaattgc gtagagcagc gttttcgcca ggttagcgcg
    13741 tgcaccaaag aactgcattt gcttaccaat caccattggg ctgacgcagc aggcaatcgc
    13801 gtaatcgtcg ctgttgaagt cagtacgcat cagatcgtca ttttcatact gcaaggaaga
    13861 ggtgacgatc gacacctgcg cggcatattt tttgaaggca atcggtaatt cttccgacca
    13921 aagaatggtc aggttaggtt ccggtgccgg ccccatagtg tgcagggtgt gcaaatagcg
    13981 gaaggagttt ttggtcacca gcgtacgacc gtccagcccc atcccgccga tcacttccgt
    14041 cgcccagatt gggtcgccgg agaacagcga atcaaattcc ggtgtacgca ggaagcgtac
    14101 catacggatc ttcatgatga agtgatcgat cagttcctgt gcctgctgct cattgaggac
    14161 gccagcttta aagtcgcgct caatgtagat atccaggaac gatgccgtgc gccccagcga
    14221 catcgcgccg ccattttgcg atttcactgc cgccagataa gcgaagtaga gccactgcac
    14281 cgcttcctgt gcattctgcg ccgggcgaga gatatcaaag ccatatttcg ccgccatttc
    14341 ctgaatctgc aacagcgcat gacgatgctc tgccagctcc tcacgcagac ggatggtggc
    14401 ttccagatcc tcgccttttt ccagacgaga ctggagatcg gcaaattgca gttcgcgttc
    14461 acgcaccaga taactgatgc catacagcgc tacgcggcga tagtcaccga taatgcgccc
    14521 acgaccatag ccatctggta aaccggtcag cacgccagat ttacggcagc gcagcatatc
    14581 cggtgagtaa acatcaaata cgccctggtt atgggtttta cgcagatcgg taaacagata
    14641 ttcaaattca ctgtccattt cacggccata ggcgtggaat gaacttttaa tcatattaat
    14701 gccaccgaac gggtgtagcg cacgtttcaa cggcgcatcc gtttgcaggc caacaatttt
    14761 ttccagcggc tggttaatat atcccgcatc atgagcggta attgtggtgg caatattggt
    14821 atcgaaatca accggcgcgt gggttgcgtt ttcgatacgg atgccttcca ttaccttttc
    14881 ccacaattcc gtggtggcag gcgtcgcttc ggcgagaaaa gattcatcgc cttcatacgg
    14941 tgtatagtta tgttgaataa aatcgcggac attaatttcg cttttccagt ccgtaccttt
    15001 aaagccaagc catgcgtcag cgtacagctt atcgctggta tcaatatcta ccttcatgaa
    15061 aaataatctc tctacaatac ttcaactaaa ttatgcaaat tctgcgggcg cgttaacttt
    15121 acctaaatga atggcatcca aagcaatcat tttttcttcg ttagtcggaa taacgacgca
    15181 aatgacatgc gcattttcac tggaaacaat tcgctcgcca aaagagttag aacgattatt
    15241 catttctgta tcaatctcta cgcctaatac agccaaatgt tccatgacca gacgacgaat
    15301 taaacttgaa ttctctccta ttccgccggt gaatataatt ccatccagcc gacgtaatga
    15361 agctgcatgt ccggcaatat gacgggcaat tcggtgaaca aaggttttaa ttgccagttg
    15421 cgcgcgttcg tgaccttcat gccaggcttt ttccagaaca cgtaaatccg aagaaagacc
    15481 ggaaatacct aataatcccg actctttatt cactacgcgt tccaggtcac ccaggctctg
    15541 gttggtttgg ctggcgaccc aggacatcgc accaaagtcg acatcgccac tgcgggtacc
    15601 catcatcaag ccttccagcg gcgtcattcc cattgaggtg tcaacactct gaccgttgcg
    15661 aactgcgcag attgacgcgc cattgccaag atgcgccaca accaggccgg agtcatcttc
    15721 cgccagattc agcagcgaat gtgcgcgctg ggaaacatag cggtgcgacg tgccgtggaa
    15781 accataacgg cgtacaccta actcttcata atatttccac ggcaggccgt ataaataagc
    15841 ttccggagcc atcgtctggt ggaaactggt atcaaatacc gccacctgag ttacgcccgg
    15901 aaataattgc tgcgccgatt caataccact taaatttgcg taattatgca ggggtgccag
    15961 tggagaaacg cgacggatat tatcaatgac ttcatcggta ataatggcgg actcggtaaa
    16021 aatactgccg ccgtgagcga tgcggtggcc aattaaggcc acgctgtcat ttaaattccg
    16081 tttttccagt tcaaatgcaa ttgccttcaa tgcaccttcg tagctgtggt gagccagcgg
    16141 tgctggctct cccccattta cggataagaa tgcattttcc gagttaatac cgtcggcaat
    16201 acctgacatt aatacttcac aatcgctggc atcgagtacg gaaaacttaa tcgaagacga
    16261 accacagtta ataaccaaaa caaccggaaa ttcattcatc tcttttctca tcctgagtta
    16321 cggattaaaa cagtttgtat acgatgttca ggatggtcag cagaccaatc acggtaacaa
    16381 acacgttatc cagacgacca cggtatttcg ccagagacgg cgctttacgg atagcataca
    16441 tcggcaacag gcacagcagg gatgcgataa tcggtgcgcc catggcttca atcaggtcga
    16501 ggatgttcgg gttggcgtag gcaacaaccc aggtggagcc catgatgaag atcatgctga
    16561 gagtattcag tttacccagc gacactttgg ttttgtcacc tttataaccg aacttcagaa
    16621 tcagaccatt caagccttcc agcgtcccca gatagtgacc gaagaaagat ttgaagatag
    16681 ccacgagtgc gatgatggaa gccgcatatt ccagtgtaat cgcgaacgtt gttttcgtac
    16741 cggtcatgga cgcaaagtgg ttagccagat aagaaagcac tggaatattc tgcgctttgg
    16801 cttccgccat gttggccgga gacagagtaa acaggcagct aaaggcaaag aacatcacca
    16861 ctgcaaccat cagcatgctg gcacgagaaa tgatttggga acatttacgt tcggtgaagt
    16921 cgcgaccgaa gtctttctca tactcttcac gtttagaaac cacgaaggaa gagacgattg
    16981 gcgagaagtt aaaggagaaa accatgatgg aaatccccag ccagacagtg atcaggatac
    17041 cgtcatgacc ggttaacgac agcgaaccga ggtcaacctg gtcgataact gcagagttcc
    17101 agtaagggat cagcgacaga gaaatcagca ccaggctggc gataaacggc cataccaggt
    17161 agctcatcac tttaaccatc agatccttac caaaccagat gacgaaagcc atcagcagca
    17221 acaggaacag cgccacaaag ccgcgattca gcggtgcaaa tccgagctgg ttttcccaga
    17281 acgtcataaa ggtattggta atagtaacgc cataaatcca cagcagtggg caaatcgcga
    17341 agaagtacag gaacgtgata accacgccgc cagttttacc aaaatgctct tccaccgttt
    17401 ccgtaatgtt gccggaaggg ttagagccag aaagacacag acgcgccagc gcccggtggc
    17461 aataaaatgc gatggggtat gccaatacca acatcagaag aatcgggatc agtccgccaa
    17521 aacctgcgcg gatagggaag aacagcaccc cggcgccgat tgccgtacca aacaagccta
    17581 acgtccatgt ggtatctgat ttacgccagg acgattgttt tgtctggctg gatacaatgc
    17641 tatctgaagt actcatatcc tatcctcaac gaattaatta agcgtcaacg aaaccggtga
    17701 tttgagagac gcgagaaaga tcgatattgc cgccggaaat aatactgacg gtttttctat
    17761 tttgaatata ttggtctaat ttaccgctta ataatgcagc acatgccaga gcgcctgcgc
    17821 cttcggtgac gactttattg cgctgaatta aggcaatcat actgtttctg atttcgtctt
    17881 cgctgaccag cacgatgtca tcgactaatt cacgaacgat ttcgtaagtt aaattacccg
    17941 ggcgggagac atcacaacca tccgccaggg tgccggtagt tcggtgcgtg gttatttctc
    18001 cggagtggaa agaagccgcc atgccgtgaa cgttttctga ctgtacgcca ataacacgaa
    18061 tggtcgggtt aatagattta attgccaccg caataccagc aattaaacca ccaccaccaa
    18121 ttggcacaat cacgttatcg acatcataga gatcttccat aatttccaga ccaatcgttc
    18181 cctggccagc aatcactttc ggatcatcgt aaggtgggat aaaaatacga ccttccattt
    18241 cgacaatttc gctcacttta gcgatagtgt cgttgaagtt atcaccatgc agaacgactt
    18301 ctgcggagta gtcgcacgtt gccgctactt tggattttgg cgcacctttt ggcatcacca
    18361 ctttaccgtc gatacccagc atcgcgcagg agagcgaaac cccttgcgca tggttgcccg
    18421 cagaacaggc caccacgcct ttgcgttttt ccgcatcggt cagtgaactt aatttattaa
    18481 atgcgccacg aattttaaat gaacccgtac gctgcatatt ttcaaacttc aggaatattt
    18541 cacctttgca acgttcacta aaatagttgg agcgaggcat gcctgtttta taaattcgcc
    18601 cagccagtcg ttgtttcgct tcaataatat catcaatagc aaccggcaga tcgtatgtaa
    18661 tatgcattat aaaacctctt cgcctgaatt aaatatgtag gtaaccgtaa ccgacacctg
    18721 caaaacagac aggtggatta tttatgttta aataaaaaca aacaactaac caacttctat
    18781 taattgcctt cgtctacacc cattataaga tgaatactct ttggctaatt ccaccaaaac
    18841 cgatgctgct tttttaatac gataattttt cgaccatacc gcggcatatt gtgccacagg
    18901 taatgtttct tcaaccggaa tagtaataaa ttgattagaa ccaaaaggtg acgtcatatc
    18961 acaaggaatt acagttaaga aatcagcatt gagaacaaga ttataaattg tcacgactga
    19021 gtcggtttta acgatgtttt caatactgat gccatttctt tgtaacgtag taagcagttc
    19081 gctgtagtac cccatattag tttgtggcaa cacccactgt tcgttcttca acgactccag
    19141 cgtggtggtg ccggtgcatg ttcgggactt actggctacc agcacaaact cggactcgaa
    19201 cagcggctca acatgtaaat cctgaagctt catttctgca cttaacgtac caatcgcaaa
    19261 atccagtcga ccgtcgcgga ttgccggtaa gaacgaagac agttgcgctt catacataga
    19321 aacctgcgct ttcgggaaca cctctttgaa cttgttgatc atccctgaca taaaagtaaa
    19381 accaatcaat gaaggaaaac caaatgagac ttccaccacc gcttcagaag acataccgct
    19441 tatctcatta accatatttt tcatttcacg ggtaatggat tcggaacggg agagtaacaa
    19501 ttgaccggca ggtgttaatg ttacaccggt atttttccgc accactaatt ccacaccaaa
    19561 ataatcttca atatcgttaa tgattttact gacggccggt tgagttaacc ctaattcttt
    19621 tgcagccgag ccgatagagc cacttctaat gacttcctga aagactacca ggtgctgcgt
    19681 tttcggaaga agaatagtgc tcataacgac ctacgttaat tacctcattg acggcatgaa
    19741 gtgtatcaaa atgaaataaa caggatatgt gcgaccactc acaaattaac tttcattact
    19801 ttccaaggta tcgttattac aattaaacat ctttaaatat caacaagtta aagcataaaa
    19861 atcagcataa accctgattt ttatcaaaaa aaaatgacgg ggataaccat attttatggc
    19921 gataacatca ttcgttatgt ggttaataca aaaaaggggc tgagaccata tttcaagcaa
    19981 ttaccggata attaccaggc aaaatttgag ttaaatttaa tgagcaagtt ctcaaatttt
    20041 attataaata aaccattttc agcgataaat actgcggcac gtcacatttt ttcacgctat
    20101 ttgttggaga acaaacattt attttatcaa tattttaaaa tttcgaatac aggtattgat
    20161 catctcgaac aattgattaa cgtcaacttt ttctcttctg acaggacgtc attttgtgaa
    20221 tgcaatcgtt ttccataaat tcttctcccc tcataggcga cgaatagcat tttgtgttga
    20281 ggatcacaaa acgaataatt gctggtcgcc gcgataaggt cagacgaagt caacaagggg
    20341 gattttcact gtgctttaca tcagcgtagg acacagaatg ttgcttcgtt tactgtaacg
    20401 ccgggtaaat gagcgttttt tgatagtgcg taagtgtcgt gcgaggaaaa taagcatcta
    20461 ttattgtttg tcatgacgac aaggcaggca tcaatgaaag gcttcccaat tgcacatatt
    20521 tttcaccctt caatcccgcc aatgcacgca gtggttaaca atcacaatag aaatattgat
    20581 tattggacgg taaaaagaaa gtttgcagaa attgtctcca ccaatgacgt taataaaatt
    20641 tacagtataa gtaatgaact ccggagagta ttatctgcaa ttactgcatt gaatttctat
    20701 caaggcgatg ttccttctgt catgattccg aatccaaccg gaaaatatga gtccattcat
    20761 catagatatt tctacaggag aacatgatga ttatatcata caaacattag atgtaggcac
    20821 ttttgcacct tttggtgaac aatgtacttg ctcagccgtc aataaaaaag agctggaatg
    20881 tattaaggag acgatttcta agtattgcgc aaagttcacc caaaaagaag ccatcttaac
    20941 accccctagg cactttaata aaacctgcat tacttcagat tgttggcaaa ttctcttttt
    21001 ttcacccgat cattttaata atgattttta ttgattattt ataaaggata aaataatgtt
    21061 ccctgtttca tcaattggta atgatattag tagtgattta gtccgtcgaa aaatgaatga
    21121 ccttcctgaa agccccatag gaaataacct cgaagcacta gcaccaggta tagaaaaact
    21181 aaaacagacc tctattcaaa tggtcacttt acttaacgcg ttacaaccaa gtggaaaatg
    21241 cattatcact ggtgattttc aaaaagaatt agcgtactta caaaatgtaa ttctttataa
    21301 tgactcgtct cttcgtctgg attttttagg ttataacacc caaattattc aacgatcgga
    21361 caatacctgc gaacttacca ttaatgaacc gttaaaaaac taggaaatat ctacaggcaa
    21421 tatcaatgtt aattgcccat taaaagatat ttacaatgaa atcaggaggt tgaacgtaat
    21481 ctttagttgt gggactggag atatagttga tctatcctct ctggacttac gtaatgtcga
    21541 tttagattat tatgatttca cagataaaca tatggctaat actattctaa atcattttaa
    21601 attggattca acaaatttta ctaatgccaa catgtttcag gttaattttg ttagttcaac
    21661 acaaaacgcc acaatctcct gggattattt actaaaaata acgcctgttt taataagcat
    21721 tagcgatatg tattctgaag aaaaaatcaa gtttgtcgaa agttgtttaa atgagcttgg
    21781 agacattacc gaagaacaat taaaaattat gagatttgca attataaaat ctataccaag
    21841 ggcaactctt acagataaat tagaaaatga attaacaaaa gaaatatata aaagctcatc
    21901 gaaaatcaac aattacttga acagaattaa attaccagag atgaaagaat tctcatcaaa
    21961 aaaaacatat gattacatcg atacaatcat tgaagattat gaaaatatca aagaaaatgc
    22021 ttatctggtc gtcccccaaa ttaattatac tatggattta aacatagaag actctagctc
    22081 agaagagtta ataccctcga gaaagacgaa aattctccgg acaatggctt tgaggtcggg
    22141 gaatataaca catatgaagc atataactca gagaatcaat attttaccag agaggactat
    22201 acgtataatt acgacctttt aaatgcaata tagacttaat aacttcttac aaagtctatt
    22261 attaataccg tagatattca tcttaccctg tgcaccaaaa gcaaaaactt atcgacgcgg
    22321 gtttgaaact gatggcaacc gcaaaaatgc ctgatgcgct acgcttatca ggcctacgcc
    22381 atctctgcaa tatattgaat ttgcgtgctt ttgtaggcag gataaggcgt tcacgcagca
    22441 tccggcatga ataaagcgca ctcaacaatc tgaaacccgc cggaacggtt tattacgtac
    22501 atcaggtgaa actgaccgat aagccgcttt cttttgggta tagtgtcgtg gacagtcatt
    22561 catctttctg cccctccaaa agcaaaaacc cgccgaagcg ggtttttacg tatatcaggt
    22621 gaaactgacc gataagccg
//
LOCUS       DAJTYM010000063        22596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTYM010000063 DAJTYM010000000
VERSION     DAJTYM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            426..1052
                     /locus_tag="OO575_002505"
     CDS             426..1052
                     /locus_tag="OO575_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6665427.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1073..3174
                     /locus_tag="OO575_002506"
                     /pseudo
     CDS             1073..3174
                     /locus_tag="OO575_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3187..3825
                     /locus_tag="OO575_002507"
     CDS             3187..3825
                     /locus_tag="OO575_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6665428.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3889..4557
                     /locus_tag="OO575_002508"
     CDS             3889..4557
                     /locus_tag="OO575_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6665429.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4554..5339
                     /locus_tag="OO575_002509"
     CDS             4554..5339
                     /locus_tag="OO575_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6665430.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5343..6155
                     /locus_tag="OO575_002510"
     CDS             5343..6155
                     /locus_tag="OO575_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665431.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6167..7589)
                     /locus_tag="OO575_002511"
                     /pseudo
     CDS             complement(6167..7589)
                     /locus_tag="OO575_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7715..8020)
                     /locus_tag="OO575_002512"
     CDS             complement(7715..8020)
                     /locus_tag="OO575_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6665432.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8128..8204)
                     /locus_tag="OO575_002513"
     tRNA            complement(8128..8204)
                     /locus_tag="OO575_002513"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8168..8170),aa:Val,seq:gac)
     gene            complement(8209..8285)
                     /locus_tag="OO575_002514"
     tRNA            complement(8209..8285)
                     /locus_tag="OO575_002514"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8249..8251),aa:Val,seq:gac)
     gene            8593..9849
                     /locus_tag="OO575_002515"
     CDS             8593..9849
                     /locus_tag="OO575_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6665433.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9890..11263)
                     /gene="mdtK"
                     /locus_tag="OO575_002516"
     CDS             complement(9890..11263)
                     /gene="mdtK"
                     /locus_tag="OO575_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6665434.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11478..12119
                     /gene="ribE"
                     /locus_tag="OO575_002517"
     CDS             11478..12119
                     /gene="ribE"
                     /locus_tag="OO575_002517"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6665435.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12159..13307)
                     /gene="cfa"
                     /locus_tag="OO575_002518"
     CDS             complement(12159..13307)
                     /gene="cfa"
                     /locus_tag="OO575_002518"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6665436.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13598..14809)
                     /locus_tag="OO575_002519"
     CDS             complement(13598..14809)
                     /locus_tag="OO575_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6665437.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            14922..15854
                     /locus_tag="OO575_002520"
     CDS             14922..15854
                     /locus_tag="OO575_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6665438.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(15851..16876)
                     /gene="purR"
                     /locus_tag="OO575_002521"
     CDS             complement(15851..16876)
                     /gene="purR"
                     /locus_tag="OO575_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6665439.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17175..17264
                     /gene="ynhF"
                     /locus_tag="OO575_002522"
     CDS             17175..17264
                     /gene="ynhF"
                     /locus_tag="OO575_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6665440.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17430..18599
                     /locus_tag="OO575_002523"
     CDS             17430..18599
                     /locus_tag="OO575_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6665441.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18745..19326)
                     /gene="sodB"
                     /locus_tag="OO575_002524"
     CDS             complement(18745..19326)
                     /gene="sodB"
                     /locus_tag="OO575_002524"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6665442.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19454..20281)
                     /gene="mepH"
                     /locus_tag="OO575_002525"
     CDS             complement(19454..20281)
                     /gene="mepH"
                     /locus_tag="OO575_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6665443.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20616..20963
                     /gene="grxD"
                     /locus_tag="OO575_002526"
     CDS             20616..20963
                     /gene="grxD"
                     /locus_tag="OO575_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6665444.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21002..21502)
                     /gene="tnpA"
                     /locus_tag="OO575_002527"
     CDS             complement(21002..21502)
                     /gene="tnpA"
                     /locus_tag="OO575_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6665445.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            21510..>22596
                     /locus_tag="OO575_002528"
     CDS             21510..>22596
                     /locus_tag="OO575_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6665446.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANA"
BASE COUNT         5852 a         5276 c         5849 g         5619 t
ORIGIN      
        1 tgatattctt gcttcagttg cggtccagtt aatcattcta aataacaact gctgaaatta
       61 tacgaatttc ggtatgtatt ttaatttata gcgataatgt tatttttatt ttctgcaatg
      121 aacgtcacaa atgattgtcg cttaagttaa atttatttca aatgataata aatatcagtt
      181 cgcttataat ttcttgcttt gtaattttaa ttgataacga tcaatgttaa gggctcagaa
      241 taatcacatt attttttctc ctaaccataa aggattagtt tatcggcgtt aaatcttttc
      301 tcatacacta tcgggttagg gaaaatatct ggtgttgggg acagagtcag cagcgtatta
      361 gttgaatgct taatatcagg ctgctgaatt gtcagcactg tgtttgcgaa gaggatgcga
      421 aataaatgaa cccggttgat cgtccactat tagatattgg cttaacgcga ttagaattct
      481 tgcgtatatc aggaaaaggc cttgctggtc taacaattgc ccctgcgtta ctgtcacttt
      541 tgggttgcaa acaggaagat attgatagcg gcacagtagg gttgataaat acacccaaag
      601 gggtgctggt gacccagcga gcgcgatgca cgggttgcca ccgctgtgaa atctcttgta
      661 ccaacttcaa cgatggctca gtagggacat tcttctcccg tatcaaaatc catcgcaatt
      721 atttctttgg cgacaacggg gttggctctg gcggcggcct gtatggcgat ctcaactata
      781 ccgcggacac ctgccgtcaa tgcaaagagc cgcaatgcat gaacgtctgc ccgattggtg
      841 cgattacctg gcagcagaaa gaaggctgta ttaccgtcga tcataaacgt tgtattggct
      901 gtagcgcctg tactacggca tgcccgtgga tgatggccac cgtaaatacc gaaagtaaaa
      961 aatcctcgaa atgtgtgtta tgcggtgaat gcgcaaacgc ctgcccaaca ggggcgttaa
     1021 aaattatcga gtggaaagat attactgtgt gaatcttgca aaggaaaaaa tcatggctaa
     1081 cggttggaca ggtaatatat taagagtcaa tctcacgaca ggaaatatta ccctcgaaga
     1141 ttccagtaag tttaaaagtt ttgtcggtgg catgggcttc ggctacaaaa ttatgtatga
     1201 cgaagtaccg ctaggcacga aacctttcga tgaagcgaat aaattagtct ttgctaccgg
     1261 cccattacct ggatctggtg ccccctgtag ttctcgcgta aatatcacct cactttctac
     1321 ttttaccaaa ggaaatttag tcgcgatgcc catatgggtg gcttttttgc agcgcaaatg
     1381 aaattcgctg gatacgacgt cattattatc gaagggaagg cgaaatcacc ggtatggctg
     1441 aagattaaag atgacaaagt tagcctggaa aaagccgatt tcttatgggg aaaagggacg
     1501 cgcgcaacga cggaagaaat ttgtcgattg accagcccgg aaacctgtgt ggcggctatt
     1561 ggtcaggctg gggaaaacct tattcctctc tctggcatgt tgaatagccg taaccacagc
     1621 ggcggtgcgg gaactggcgc aataatgggt tcgaaaaacc tgaaagcgat tgcggttgaa
     1681 gggacgaaag gggtcaacat tgccgatcgt caggagatga agcgtctcaa tgattacatg
     1741 atgactgaac ttattggtgc gaataacaac catgtcgtgc caagtacgcc acaatcgtgg
     1801 gcagagtatt cagatcccaa gtcacgctgg acagcacgta aagggctgtt ttggggcgcg
     1861 gctgaaggtg gtccgattga aacgggtgaa attccgccag gcaatcagaa tacggtcggc
     1921 tttcgtacct ataaatccgt ttttgactta ggaccggcgg cagagaaata cacagtaaaa
     1981 atgagcggct gccactcttg cccgatccgt tgtatgaccc aaatgaatat tcctcgggtg
     2041 aaagagtttg gcgtgcccag cacaggtggt aacacttgtg tagcaaactt tgtccatacc
     2101 accatcttcc cgaacgggcc gaaagatttt gaagataaag acgatggtcg tgtgattggt
     2161 aacctggttg gtctgaattt gttcgatgac tacggcctat ggtgtaacta cgggcagttg
     2221 catcgcgact ttacttattg ttacagcaaa ggtgtgttca agcgtgttct gccagctgaa
     2281 gagtatgcag aaattcgctg ggatcaactg gaagcgggtg acgttaactt cattaaagat
     2341 ttttactacc gtctggcgca tcgtgtgggt gagctgagtc acctggctga tggttcatat
     2401 gccatcgcag aacgctggaa tttgggtaaa gagtactggg gctacgcgaa aaataaactc
     2461 tggtcgccgt ttggctatcc ggttcaccat gccaatgaag cgtcagcgca ggtcggttcc
     2521 attgttaagt gtatgttcaa ccgtgactgc atgacgcata cccatatcaa ctttattggt
     2581 tccggcttgc cattgaaact gcaacgtgaa gtggcgaaag aactttttgg ttctgaagat
     2641 gcttacgatg aaaccaaaaa ctacacgcca atcaatgacg caaaaattaa atatgcaaag
     2701 tggtcgctgt tgcgggtctg tttgcataac gccgtcactc tgtgcaactg ggtctggcca
     2761 atgaccgttt cgccgctgaa aagccgtaat taccggggcg atctggcgct tgaagccaaa
     2821 ttcttcaaag cgatcaccgg cgaagagatg actcaggaaa aattagattt agctgcagag
     2881 cgtatcttta cgttgcatcg tgcctacacg gtaaaaccga tgcaaaccaa agatatgcgt
     2941 aatgaacacg atcttatctg ttcctgggtt ttcgacaagg atccgcagat cccggtcttt
     3001 actgaaggta ctgacaaaat ggatcgtgac gatatgcatg cttcgctcac gatgttctac
     3061 aaagaaatgg gctgggaccc acagcttggt tgtccaaccc gcgaaacatt gcagcgtctg
     3121 gggctggaag atattgccgc cgatctggca gcacacaatc tactgcctgc gtaaggaatg
     3181 ggtaagatga accatcagga tgaattgccg ttagcgaaag tcagcgaagt tgacgaggcg
     3241 aagcggcaat ggttgcaggg gatgcgtcac cccgttgata cggtaaccga accagaacct
     3301 gctgaaatcc tggctgagtt cattcgccag cattctgcgg ctgggcagct ggtggcgcgc
     3361 accgtgtttc tctcgccacc ctattcggtt gcagaagagg aactgtcagt tttgctggaa
     3421 agcattaagc agaacggaga ctacgcggat atcgcttgta tgacgggaag tcaggatgat
     3481 tactattact caacgcaggc catgagtgaa aactacgcgg cgatgtcttt gcaagttgtg
     3541 gagcaggaca tttgtcgcgc cattgcacac gccgttcgtt ttgagtgcca gacgtatcct
     3601 cgtccttaca aagtcgccat gctgatgcaa gcgccatatt acttccagga agcgcagatt
     3661 gaagccgcga tcgccgcgat ggatgttgca cctgaatatg ccgatatacg gcaggtggag
     3721 tcatcgacgg cggtgttgta cttgtttagc gagcgattta tgacctatgg caaggcttat
     3781 ggcttatgcg aatggttcga ggtagagcag tttcagaacc cctgaaattt cagggagatg
     3841 tcatggatag ggtggacagt tgcagccact gcattagggg ataatcagat gtccttcact
     3901 cgacgcaaat ttgttctggg gatgggaaca gtaatctttt ttactggttc tgcgtcttct
     3961 ctgttagcga acacgaggca agaaaaggaa gttcggtatg ccatgattca tgacgagtca
     4021 cggtgcaatg gctgtaatat ttgcgcccgc gcatgccgta aaactaacca cgtccccgct
     4081 cagggaagcc gtttatcgat agcacacatt ccagtcaccg ataacgacaa cgaaacgcag
     4141 tatcactttt ttcgccagtc atgtcagcac tgcgaagatg ctccgtgcat cgacgtatgc
     4201 ccgacggggg catcgtggcg tgatgagcag gggatcgtgc gggtagaaaa atcgcagtgc
     4261 atcggctgca gttattgcat tggcgcttgt ccgtacctgg ttcgctacct taaccctgtc
     4321 actaaagtgg cagataaatg tgatttctgc gctgagtccc gactggcaaa aggctttccg
     4381 cccatttgcg tcagcgcctg tccggaacac gcattgatct ttggtcgcga agatagccca
     4441 gaaattcagg cgtggttgca ggataataaa tactatcaat atcagctacc tggtgccgga
     4501 aaaccgcatc tgtatcgccg gttcggtcaa catttgatta aaaaggaaaa tgtatgaacc
     4561 cgtcgcaaca tgctgaacag tttcagagcc agttagcgaa ctatgtgcca cagttcactc
     4621 ccgaattttg gccggtgtgg ttgatcattg ccggagtatt gctggttggg atgtggttgg
     4681 tgctggggct gcacgccttg cttcgtgctc gtggcgtgaa gaaatcagcc accgatcatg
     4741 gtgagaaggt ttatctttac agcaaagcgg tcagattatg gcactggtcg aatgcgttac
     4801 tctttgtatt gttgctggca agtgggctga taaatcactt tgcgatggtg ggcgcaactg
     4861 cggttaaaag tctggttgcg gtgcatgaag tttgcggatt tttgttactg gcatgctggc
     4921 tcggctttgt gctgatcaat gccgttgggg ataatggtca ccactatcgc attcgtcgtc
     4981 aggggtggct ggaacgagcg gcaaaacaaa cgcgatttta tttgtttggc attatgcagg
     5041 gggaagaaca tcctttcccg gcaacaacac agtctaaatt taatccctta cagcaggtcg
     5101 cctatgttgg tgtcatgtat ggattgctgc cgttgttact attgacgggg ctgttgtgtc
     5161 tctatccgca agccgtggga gatgtgtttc ctggcgtaag atactggtta ttgcaggcgc
     5221 attttgctct ggcatttata agcctctttt ttatcttcgg tcatctttat ctttgcacca
     5281 cggggcgtac gccacacgaa acctttaaaa gcatggtcga tggctatcac cggcactaac
     5341 gaatgatcat tacacgagca gacctgcgag aatggcgtat cggagcggtg atgtatcgct
     5401 ggttcctgcg ccatttccca cgcggcggta gttatgccga tattcgtcat gcactgattg
     5461 aggaaggata tacggactgg gcggagagtc tggttgagta tgcgtggaaa aaatggctgg
     5521 cggatgaaaa cttcgcgcat caggaagttt cctctatgca aaaactggcc acagaccccg
     5581 gagagatacc attttgcagt cagtttgccc gcagcgacga tcatgctcgc atcggctgtt
     5641 gtgaggataa cgcccgaatc gcaaccgctg ggtatgccgc gcaaattgcc agtatgggtt
     5701 atagcgtgcg gattggaagt gtcggcttta acagtcacat tggcagctcc ggtgaacgcg
     5761 cacgggttgc tgtaaccggt aattcttcgc ggataagcag cgctggagat agtagtcgga
     5821 ttgcgaatac cggaatgcga gtacgcgttt gtacattggg tgaacggtgt catgtcgcca
     5881 gtaatggtga cctggtgcaa atcgccagct ttggcgcaaa cgccagaatt gccaacagcg
     5941 gcgacaatgt acacatcatt gccagcggtg aaaattcaac ggtagtgagc acgggcgtgg
     6001 tcgactcaat tattctcggg ctagggggaa gcgccgcact ggcatatcat gacggcgaaa
     6061 gagttcgctt cgccgtcgcc attgaaggtg aaaacaatat tcgaaccgga gtccgctatc
     6121 gtcttaatga acagcatcag tttgttgagt gctgatagct ttacctttaa tcaaacgaaa
     6181 gacgccgacg agcacgggat gcaggcttta cgaccgcccg agccatcgcc tcaccaattt
     6241 ctgcacatgc cgtaagtccc tgaacgaaag gctggtcgtg catcaaccag ggcaacgcgc
     6301 cgaacgctac gcgtccacga atatcttcgg gttgctgtaa cgtataatct tcgccaacat
     6361 cagggatttc agatccccgc ttcacgtaag gccagtagtc gacgaataga ctctgaaggg
     6421 attgccgcat aattatcgat gaaaaccctg gcaaggcctt tactgaaccg tttatggtcg
     6481 tgttcattca gatgaggaac aatttcctgt acggcttcat gcaggcgcag aatgacataa
     6541 cgccagggaa cagtatgttt ttctcgttta ttgcgttcga cttcctggag atttttttct
     6601 gcccagtcaa atgggtcgcg ttgtttgcgc tcggcaaacc aggcttgagc aaaggaatcg
     6661 acattcaggc tctctaaggc tatgcgttgg ctccagtctg gatcagcaaa cttgatttcc
     6721 tctactatca atctaaatac ccgatccaaa aggccctctt cacctttttg aatctcagca
     6781 tttaatgcct gatcggtgac gatgtgtaag ggctcgtagg gaatagggca atagaaatcg
     6841 gcttcgggta aaatacccgt gcgcgacatc aacgtaatat ttagcttttc acttgcgtta
     6901 tcgcggtgaa aaacgacgtg ttgtttatca tcttcaatga acgaaccatg ctgaatagcc
     6961 actgccattg ccgcatccag tccgctcaag gatgttccca taatacccac gttacacgca
     7021 tcgacctttg cttccatcag gcctgaccac gggctgggaa aatacgttct ggttgcttct
     7081 tcttcatcag gccagacgtg acccgtggca atcaccgcta aatcaaacgt ctcgctgggt
     7141 aaatcctgat ttgtagcgag catgacgcca gcatttgtaa tttgcagatc ggtaacctgg
     7201 catgattcat aaacagccac tgcaaatttt tgctgtcgtg cctggtctac taatcgtaaa
     7261 aattgatcgc ggaaatattc gcccagcaga attcgcggta aaaactgacg atcgtgcaag
     7321 gtttcttttt taacgccata acgctggaga tggctggctt cttgcttttg tagccattcg
     7381 agatacgtac aattaatcgg cggtatttca atactggcaa tatttgccag catcattttt
     7441 gagttttcct catcactgta tggcatcccg acaccggcct cgtcagcctg ctcgaagata
     7501 gaaatagaaa gtggagtttg ttgctgtaga agcgagaata aggtgtagat ccccgtaggc
     7561 ccggcaccca caatagcaat ttttttcatt gttacttacc tgtacgcatt ctttcgaatt
     7621 tagtgttatc agataagttt ggttgagatt tttagaattt gagaaatttc agacagggat
     7681 gaaaagggcg ctaaccgccc ttgattaaat tctgtcaggc gagttttgcc tgattgattt
     7741 gactaagtgg ctcattgaca tcgaaaactt tggcgacaac ttcctcaacg ccgagatttt
     7801 tcagtcgggc agtatgtttt tccagatagg caagggcgct tttttcatca gtgaaaaggt
     7861 agatcccacc ggcttcgtgg ttcttttcac tttctgtcca taccttccac agaaaaccag
     7921 gttcctgatt aatcgactca gcaagtggtt taagctgctc agccattgcg tcgccaaatg
     7981 ggccattaaa agcaaaatga agttgtaaca aagttgccat agcgattctc cttctgataa
     8041 cgcatacgaa gacagtgtaa atcttttaac gtattggata cagagaagaa acagagggaa
     8101 aaaggaaaag accagattaa gaaaatctgg tgcgtccgag tggactcgaa ccaccgaccc
     8161 ccaccatgtc aaggtggtgc tctaaccaac tgagctacgg acgcaggatg gtgcgttcaa
     8221 ttggactcga accaacgacc cccaccatgt caaggtggtg ctctaaccaa ctgagctatg
     8281 aacgcaacgt tgtaggtgac aacggggacg aatattagcg gcagagtgga aaggtggcaa
     8341 gaggcaaaac gtaattttct gcgctatttc gaccgtttgc agagctttta agcaaattgg
     8401 ctatattttg ttgatttgca agggcgattt ttattcagga tcgcatttac atctgataca
     8461 acccaataat tcgtgtatca gagtttaaac agagttgttt gccaccatct caaatctgtc
     8521 gtggataaat cctccgtagc ctttacactt aggtagcaat aaaggcacac agatgcagca
     8581 aaggaataaa tcatgggatc tgatgcgaaa aacttgatga gcgacgggaa tgtgcaaatt
     8641 gttaagaccg gcgaggtcct tggcgcgacg caacttactg aaggcgagtt gattgttgaa
     8701 gctggcggaa gagccgaaaa taccgtggtc acgggggctg gctggttgaa agtggcaacc
     8761 ggtgggatcg ccaaatgcac acagtacggt aacaatggca cgctatcggt cagcgacggt
     8821 gccattgcca cagatattgt tcagtccgag ggaggcgcaa ttagtctctc tacgctcgct
     8881 acggttaatg gccgccatcc cgaaggtgaa ttcagcgttg atcagggtta tgcctgcggt
     8941 ttgttgctgg aaaatggcgg taacctgcgt gtactggaag gacatcgcgc ggaaaaaatc
     9001 attctcgatc aagagggcgg cctgttggtt aatgggacaa cctcagcggt cgtggtagat
     9061 gaaggtggtg aattgttggt gtatccaagt ggagaagcca gcaattgtga gattaatcag
     9121 ggcggcgttt ttatgctggc cgggaaagcc agtgatacgt tgcttgctgg tggcaccatg
     9181 aataatctcg gtggtgaaga ctctgacact attgttgaga atggatccat ttatcgtctg
     9241 gggacggatg gccttcagct ctacagttcc ggtaagacgc aaaacctgtc ggtgaatgtg
     9301 ggggggcggg ctgaagtgca tgccggtacg ctggaaaatg cggtaataca aggtggaaca
     9361 gtgatcctgt tgtcacccac cagcgcggac gaaaattttg tcgtagagga agatcgcgca
     9421 ccggttgaac tgaccgggag tgttgcatta ctggacggcg cttcaatgat tattggttat
     9481 ggcgcagagc tgcaacaatc aacgattact gtacagcagg gcggtgtgtt gattctcgac
     9541 ggcagtacgg taaaaggtga cagcgtcact ttcattgttg gtaacatcaa tctgaatggc
     9601 ggaaaactgt ggctgatcac tggtgcggca acgcatgtgc aactgaaagt gaaacgcctg
     9661 cgcggagagg gagcgatttg cctgcaaacc agtgcgaaag aaatttcacc tgacttcatc
     9721 aatgtgaaag gggaagttaa cggggatata cgcgttgaga taacagatgc cagtcggcaa
     9781 actctgtgta acgctctgaa attacagcca gacgaagacg ggattggcgc aacgctccag
     9841 cctgcgtaaa aacacaggcg caacccgtga aggttgcgcc ttgtctttat tagcgggacg
     9901 ctcgttgcag aatgatggct gacggcatac gttgcaggaa ccgcatacgc agcatcatca
     9961 taatggctgc cgatgtcagg ccgataataa agcctatcca gaagcctgct ggccccatag
    10021 gttcaacgac cagatcggtc agtgccagaa tatagccgct tggcaagccc agcacccagt
    10081 aagctgtaaa ggtgatatag aaaatggaac gcgtatcttt ataaccacgc aaaatcccac
    10141 tgccaatcac ctggattgag tcagaaatct gatataccgc cgccagcaac atcaaatgcg
    10201 cagccagcgt tacaacctcg ggattgtcgt tgtacaacag ggcgatttgc tcccgcagtg
    10261 aaaccgtgaa aatggccgtc agggttgcca tacagacacc caccataagc ccggtccgcg
    10321 cagcggtttg cgcatccagc gttgagccct gacccagacg ataacctacg cggatagtta
    10381 ccgctgccgc cagcgacatt ggaagcacaa acatcagtga actaaagttc agggcaatct
    10441 ggtgtcctgc gacatcaaca ataccgagcg gagacactaa cagagccacg acggcaaaca
    10501 gtgtcacttc aaagaacagt gccagcgcaa tcggcaaacc gagttgaatc agtcgtttca
    10561 taaccgcagg ctcgggctta gcggtgcctt tttcgttacg aatatcgcgc atggagcggg
    10621 cgcgtttaat gtaagaaacc atggcaagga acatgaccca atacaccgcc gcagtagcca
    10681 cgccacaacc aacgccaccg agctcaggca taccgaaatg accataaata aagatatagt
    10741 tcaccgggat gttcaccagc aggccgataa agcccattac catacccggc ttggtttttg
    10801 ccagaccttc acattggtta cgggcaacct ggaagaacag atatcccggc gcgccccaca
    10861 acaacgcacg cagataaccc acggctttgt ccgccagagc cggatcgatg ttttccatgt
    10921 agcggataat gtaacctgca ttccacagca ccagcataat gagaacggaa acaaaacctg
    10981 ccagccagaa accttgtcgc acctgatgcg caatgcgctc acgtcgaccg gaaccattta
    11041 attgcgcgat aaccggcgtt aatgccagca gcagtccgtg accaaagagg atcgccggaa
    11101 gccagataga agtaccgata gcgaccgccg ccatgtcggt ggcactatag ccgcccgcca
    11161 tcacggtatc gacaaaaccc atcgcagttt gggcgatttg cgcgagaatc accgggattg
    11221 ctaatgctaa taacagacgc gcttcactga tatacttctg cacgtgaaca ccttttattt
    11281 gtagttatat gagagactaa aaagccgcct gaatgggcag caaaaagaag aagcagggga
    11341 aattcagtct attgtagcga ggatttatta attctccagt gaaaaaatag ccattcttac
    11401 cccttcactg gtaagggcgt tttccacctg ctattgtgct gaacagaatg tcttaactga
    11461 tttcaggagt tgtaagtatg tttacgggga ttgtacaggg caccgcaaaa ctggtgtcga
    11521 ttgacgagaa accaaatttt cgtacgcatg tggtggagtt acccgaccac atgctggacg
    11581 gcctggaaac cggtgcttcc gtggcgcata acggttgctg cctgaccgtg acggaaatta
    11641 acggcaacca tgtcagtttt gacctgatga aagaaacgtt acgcattacc aatcttggcg
    11701 atttaaaagt gggggattgg gtaaacgttg agcgtgcggc gaaattcagt gatgaaattg
    11761 gcggacactt aatgtcaggt catattatga ccaccgctga agtggcgaaa atattaacct
    11821 cggaaaataa tcgccagatc tggtttaaag tccaggatag tcagttgatg aaatatatcc
    11881 tgtacaaagg atttattggc atcgacggta ttagcctgac cgtcggcgaa gtcacgccaa
    11941 cgcgtttttg cgtccattta attccggaaa cactggaacg cacgactctt gggaagaaaa
    12001 aacttggcgc acgcgtcaac attgaaatcg atccacaaac tcaggcagtg gtagatacgg
    12061 tagaacgtgt gctggcggca cgagaaaatg ccatgaatca accaggtaca gaagcctgat
    12121 ctgaactcgc ccccgaagag ggggcgatag aatagccttt agcgagcgac tcgaaggccg
    12181 ttttcaacac cgcgtgagaa caccacctgc cagagctgaa tatcacgggc gcggaaagca
    12241 cctgcgcagg cattcagata ataggtaaac atccgtttaa agcgttcact gtagttatcc
    12301 gcaatttctg gccatgcggc gaggaatcgt tcataccacg ccatcaacgt agtatcgtaa
    12361 tcagcaccga agttatgcca gtcttccatc acaaagtggg gttcgctgga ttgagcaatc
    12421 tggcgtacag agggcaggca accgttcgga aaaatatatt tattaatcca gggatcaaca
    12481 ttcagatcgg tttttttcga accgatagta tggagcagga atataccttc cggtttcaaa
    12541 ttacgatcca ccaccgcaaa ataggtatcg taatttttcg gtccgacgtg ctcgaacatc
    12601 cccacagaaa caatacgatc aaactggtcg ttcaggtcac gataatcttg cagcaaaatg
    12661 gtgacatcca ggcctttaca gcgttcctga gccatttttt gctgttcggc agaaatggtg
    12721 acgccgacca cgcttacgtc atagttagat gccatgtagt gcgctagtcc gccccagccg
    12781 cagccaatat ccagtacgcg catccctggt tttaactgca atttttcaca aatcattttg
    12841 agcttcgcct gctgggcaga ttccagatta tcggcatctt tccagtaagc gcaggaatat
    12901 tgcatgaagg gatcaagcat gcggctgaac aagtcattac ccaaatcgta atgctccttg
    12961 ccgactatcc aggcacgttt tttactctgc agattgaaga gacgagcgcc ggcaatacgc
    13021 agcgtgtctt tgaaatgatg ggggagttgg ttctcgagac ctgcgcgtaa gactttgcta
    13081 aaaaacatat ccagtcggtc acattcccac cagccatcca tataactttc gcctaacccc
    13141 aaagagcctt cctgcagaac gcgtttaaaa aaatcggggt ttttcacacg aatatccgcc
    13201 ggggcagaac cgttaatggc tataccggca cggctaagta attcgttggc gatacggtac
    13261 cagttgtcat ccggtacact gacttcttct atacacgatg aactcatagt ttctccatca
    13321 ctggtcggtg atcagaacct taaaacagcg tagacgcttt ttgggctttg tgagaaatct
    13381 cgcagaaaaa accgcgagtc tataatccga cgtagaacag agggaaagga ggaacccttg
    13441 ccgaaatgac catccatagt gagtcgggaa cgatcctgtc cccgtaaaaa cataatattt
    13501 atcgtagctt taacaactcg aaattagtat aggccgctaa tttcaatcat acaacaaaat
    13561 tttgttagca gcctaagtat aagtatatcg atatagatca gtgtgattcg ctatgagcga
    13621 cttcggcatt gccatgattc gggcagtcaa cttcttcaca acgttgcatc atgtaaccca
    13681 gcgcgaccag cactactgtt gataacatca cgctggtggt ggtgagcaat ggcgtgctga
    13741 tactaataag ccaggaaact accagacttg cgaggaagca cagacccagt tgcagagtgt
    13801 tctgcaacgc tgcggcgcga ccggttgcat gtgggaaggg acgcagcgcc tgggcgacaa
    13861 caatagggta gatcgcgcca ttggcaatcg ccatcacaca gaatgggatc aggatttcga
    13921 ccagcgacac atggctaatg aagcccgcag cccaggtcgc aatgacgctg acagcaaaca
    13981 gcaccagcaa ccacggtaat aactgcttgc cttgccattt ctgcagtgcg gcgcgacagc
    14041 cataaccacc aatcagaaac gcgatagttt gcgggacata acttaaacca ataactgccg
    14101 ggctgtagcc catttcacta aggatgaacg gtgaaccggt cagccatgca aaaaaactgg
    14161 ctgaacaggc tgcgtatatc agcacgttgc cgcgataggt tttagaacgt agcaggtcgg
    14221 taaaggtcag accatcctga ctattgttac gggccttcgt cgtgggtttg agccagaaaa
    14281 taggcagaat cagcaccacg gtaatggcaa acagggtggc gaaaatcgcc tgccaggaaa
    14341 aatggaccag cagccagctt cctaacaggg gagccagtgc cggagacaga cccaccagcg
    14401 gcatgatggt cgcaaaaata cggttgactt tctgtgaagg ataataatct gtcactaatg
    14461 cttgccagat aaccgccgcg gcgcagaccc ccacagcctg tacaaaacgc aataccagca
    14521 gcgtggcggc gttttctacc cacagcatcc ccagactacc taacgcaaaa attgtcaggc
    14581 cgattaataa caccggttta cgaccataac ggtcggagag tggcccccac agaagctggg
    14641 ctgcggcaaa accggcaagg aacagactaa ggctggcact gacagcagac gcaggcgttt
    14701 gcaggtcggc ctgtatggcg gcgaaagcag gcagatacat atcggttgcc agaaaaccga
    14761 gtacgctcaa acccgccagc cagactaaaa atcttttccc aggttgcatg tatattctct
    14821 aaatctctca attaaacacc gccgcaaagt gtagggagtg aaattagtgt tgtgaaacgg
    14881 taatatttga tgactggttt caaaaatttt gcaggcagac tatgtggtca gaatattcac
    14941 tcgaagttgt tgatgcggta gcgcgtaatg gtagttttag cgctgcggca caggagctgc
    15001 atcgtgttcc ttctgcggtc agctataccg tgcgtcagct ggaagagtgg ctggcggtac
    15061 cgctctttga acgacggcac cgtgatgtgg agctgaccgc tgctggagcg tggtttctca
    15121 aagaagggcg ctctgttgtc aaaaaaatgc agatcacccg ccagcaatgt cagcagatag
    15181 cgaacggctg gcgcggtcag ttagctatcg cagtggataa tattgtcagg ccagaacgta
    15241 cacggcagat gatcgttgat ttttatcgcc attttgatga tgtcgaactt cttgtctttc
    15301 aggaagtgtt caacggtgtc tgggatgcgc tttccgacgg gcgcgtggaa ctggcaattg
    15361 gcgcaacacg cgcgattccg gtaggcggtc gttatgcctt ccgggatatg gggatgctaa
    15421 gctggagttg cgttgttgcc agtcaccacc cgctggcgtt gatggatggc ccgttcagcg
    15481 atgatacgtt gcgcaactgg ccgtcgttgg tgcgcgaaga cacctcgcga acgctaccta
    15541 aacgtattac ctggttgctg gataaccaaa aaagacttgt cgtacctgac tgggaatcat
    15601 cggcaacctg tatctcggca ggattatgca tagggatggt gccaacacat ttcgccaaac
    15661 cgtggcttaa tgaggggaag tgggtagcac ttgaactgga aaaccccttc ccggattcgg
    15721 catgttgtct gacatggcag caaaatgata tgtcgcctgc gttaacctgg ctgcttgagt
    15781 atctcagcga tagtgagacg ctgaataagg agtggctgcg ggagccggaa gagactcccg
    15841 caacgggtga ttaacgacga tagtcgcgga acgggccgtc agccacggag cggcgttcaa
    15901 tcaaacgcgg atgcacttca atggactgcg gttcttcacg tttgttgacg atacgatcca
    15961 acagcatgtt gaacgctgtt tcacccagcg aatcttttgg ctgatggatc gtggtcagcg
    16021 ccggcgtaaa atagcgcgcg ttgcgcacgt tatcataacc gatcagcgaa acatcctgcg
    16081 ggacgcgcag gcccatttca tcagcagcac aaagtgcgcc cattgccatg atatcgccac
    16141 cacagaagac ggcagtaggg cgatgcggct gcgacaggat ttgctgcatg gcgcgataac
    16201 cggattcagg ttcaaagtca ccctgcacaa tccagctttc cggcaccttg atcatcgctt
    16261 cttccatcgc cttcataaaa ccggcaaggc ggcctgcgcc ggtgttacgt tccagcgggc
    16321 cggggatgac gccgatttcg cggtgaccgc gttcaatcag ataacgcccg gccatgtagc
    16381 cgccttcgaa cgcgttatca atgaccgcat cggtgaagtc agcttttgct tcaccccagt
    16441 ccatcaccac cattgggata tggcgatact cttccagcat cgccagcaac ggctctgggt
    16501 actcagaaca catcaccagc agaccatcga cgcgtttttg cgccatcatc gacagataag
    16561 cccgctgttt ctcaagattg ttccacgcat tacccagaat cagggtgtaa cctttctgga
    16621 agcaattttt ttcaactgct tcaatgatct cggcaaaata ggccgcttcg ctgctggtcg
    16681 ccagcaaacc gatagacttg gtgtggttaa ccttcaggct acgcgccacc gcgctagggg
    16741 agtagtgtaa ttctttaatc gctgcccaca cggcgttgcg cgtttcttca gcgacgaaac
    16801 gtgttttgtt gatcacgtgt gacacagttg tagtggaaac gtttgctcgt ttcgctacat
    16861 cttttattgt tgccattcca tttcactcca gaccctaact tcagctcctg caaaatcgca
    16921 aggtaaacgt ttgcctttac acaccttacg cacaacaaaa gtgttgcggt acgccggaaa
    16981 aacgacacgg agcgtcagga aggggtcaat ggccagtacg caaatagtgt aagcgtggaa
    17041 ttttggccga tcttgacgaa aaggggaagt ggtaaaaaca cttatcatta taaatgacgg
    17101 gagatttttt catcacagtg tgtaaaaatg cgtaatatca ctttttgtgt ggtaataaaa
    17161 aaggagaaaa cttgatgagc accgacctta aattttcact ggtaacaacg gttatcgtcc
    17221 tcggtttgat cgtagccgtg ggtttgactg ccgcgctgca ctgatttcct ctcgagggag
    17281 cgcgttctcc ctcgcatctc cttcctgttt gttagcaaaa tggcaaaagt gttttctctt
    17341 tttgttattt tcattttttt gtgattgctg tcatgctttc gccactttaa tgaatatcgc
    17401 gtaatgacgc gaagaggtgg agataaggca tgaaaattaa ctatccgttg ctggcgctgg
    17461 cgattggcgc gtttggtatc gggacaacgg agttctcgcc aatgggcttg ttgcccgtca
    17521 ttgcgcgcgg tgtggatgtc tcgattcccg ctgccggaat gttaatcagt gcctatgcag
    17581 ttggcgtaat ggtgggcgcg ccgctgatga cgcttctact ttctcatcgt gcccgccgca
    17641 gtgcgttgat tttcctgatg gcaattttca cgctaggcaa cgttctttcc gccatcgcgc
    17701 cggattatat gaccctgatg ctttcacgta ttttgaccag cctgaatcac ggagcatttt
    17761 ttggtttggg ttcagtcgtg gccgcaagcg tggtgccaaa acataaacag gccagcgcag
    17821 ttgccactat gtttatgggg ttaaccctgg caaatatcgg tggcgtgccg gcggcgacct
    17881 ggttgggtga aaccatcggc tggcggatgt catttctggc aacggcgggg ctgggagtga
    17941 tttcaatggt aagtctgttc ttctcattac ctaaaggtgg tacaggggca cgacctgaag
    18001 tgaaaaaaga gctggcggta ttaatgcgtc cgcaggtgct gtctgcattg ctgacgacgg
    18061 tactgggagc tggtgcaatg tttaccctct acacctatat ctctccggta ctgcaaagta
    18121 ttacccacgc aacaccggtg ttcgtcacgg caatgctggt gctgattggt gtcggattct
    18181 ctatcggtaa ctatctcggc ggcaaactgg cagatcgttc agttaacggc acgttgaaag
    18241 gctttttgtt gctgctgatg gtgattatgc tggcaatccc gttcctggcc cgcaatgagt
    18301 tcggcgcagc tattagcatg gtggtgtggg gcgcagcaac ctttgcggtt gtacctccgt
    18361 tacagatgcg cgtgatgcat gtcgccagtg aagcgccagg tctgtcttca tcagtcaata
    18421 ttggtgcctt taatcttgga aatgcgctgg gagcagctgc tggtggtgcg gtaatttccg
    18481 ctgggctggg atacagcttt gtgccggtga cgggggcgat tgtcgcggga ctggcattat
    18541 tgctggtgtt tatgtcagcc agaaaacaac ctgaaacagt ttgcgttgct aacagctaat
    18601 taaacaaaaa cgcagaagga taaccttctg cgttacagtt gctgacaacg tgcgatttat
    18661 cttgccgaat gcgctgttaa cgcctcatcc gcgttggcat ggtaagcgaa gcgcatcagg
    18721 caatgctgca tttgccatca gttattatgc agcgagattt ttcgctacga attcccagtt
    18781 caccagcgcc cagaagtgct ccagatagcc aggacgtgca ttgcgatagt cgatgtaata
    18841 agcgtgttcc cagacatcaa cggtcagcag cggagtcgca tcggtggtca gcggagtacc
    18901 cgcgttagag gttgaaacga tagccagttt gccatcgctg tttttcacca gccaggtcca
    18961 gccagaacca aagtttttga tcgctgcatc agtaaactgc gctttgaaat cggcaaagct
    19021 gccaaaagat gcggcgatag cttcagcgac ttttccagtc ggttcgccac cggcgttcgg
    19081 tgccaggcag ttccagtaga aagtatggtt ccagacctga gctgcgttgt tgaatacgcc
    19141 accttcagag ctgcgaataa tctcttccag tgatttacct tcaaacgcgg tacctttaat
    19201 caggttgttc aggttagtga cataagtctg atgatgcttg ccgtagtgat actcgatagt
    19261 ttccgcagaa atgtgcggtg ccagagcatc tttagcatat ggtagtgcag gtaattcgaa
    19321 tgacattgct actctccttt attattaatt tgcatacgta caatagcctt attgtgcgta
    19381 tggatagggt agcaaattaa aaggtgagac aaaagatgaa cttaccctgt tgccgtaaca
    19441 acagggtaaa gttttagcga agtgtttttg gggtcattac ccgacgagcg ccaacatagt
    19501 ggcgctgcca gtagtcttca ctgagagaag tgatttgaat ttcctgacct gtgcgcggtg
    19561 actgaataaa tttgccgttg ccgacataca cgccgacatg atcggctgta ccgcgtccct
    19621 gagtacggaa aaagaccagg tcgccgtttt tcagctcact acgttcgata ggtcctgcat
    19681 cacgcaggtg atacatttca ttcgccgtac gcggaatacg aattttcacc aaatctttat
    19741 aagcgtaata aaccaggccg ctgcaatcaa aaccggtacg cggtgagctg ccgccccaac
    19801 gatatggctt accaatttgc tgcatcagtt tattcattgc cactttcgtg gctttttgca
    19861 ccttcgcttt gtgcgcgtcg gcaagggttc ctgacgcggc atttttgact ttcacacaat
    19921 gcgatttata gcccttacgt ttggtacact tttcggtaaa gctggctgag gccgttttat
    19981 tgacggcgtg tttgctgcga gttttggcgt ttttagaagc ggtaatggaa gattttttaa
    20041 cagaagaagc ggttttgctt ttggtcatac ttttactggc ggcttttttc gctgttttct
    20101 ggctggcttt ctgggtcttt ttggtggttg ccgtgctttt ctttttatct gcttttttgg
    20161 tgatatgagt ggtagcagaa ctctccctgg cttgctttga agcatgggcc ataggcgtta
    20221 aaggcagggt ggtaaaaagt aaagcacaga gcgtgatcga aatacggttt atccgcgcca
    20281 ctgagcgtcc ctctgttaag gcaggtctat catgcctgcg tctgatttac aaaacttgat
    20341 gattctaatg catagaagaa gagaaagtta atagctttta ttgttaaaaa atacgtttcg
    20401 ttggcgagcg caaaaaaatg cagcagtgaa agctaaaaat ggttataaat acgccagttt
    20461 gccgcgcatc agagaagaaa cgaatatttg aaatgcaaca aatagagcaa agggataaaa
    20521 tagcagaagc gtgatgaaaa tgttattttc cgatgccgca ctgacccgct acaatgtcag
    20581 attactgccg taaagaagtt tgaggaagca agacaatgag caccactatc gaaaaaatcc
    20641 aacgccagat tgctgaaaac ccgatcctgc tgtacatgaa aggttcaccg aaactgccga
    20701 gctgcggttt ctctgcccag gcagtccagg cgcttgccgc atgtggcgaa cgttttgcct
    20761 atgttgatat tctgcagaat ccggacattc gtgcggaact gccgaaatat gctaactggc
    20821 cgaccttccc gcaactgtgg gttgacggcg agctggtcgg cggttgtgat atcgtgatcg
    20881 aaatgtatca gcgtggcgaa ctgcagcagc tgatcaaaga aactgccgct aaatacaagt
    20941 ctgaagagcc ggacgcggaa taatccgtcc tgcaatactg acggatgcga acgggcatcc
    21001 gtcagtcctt tatccggtgc gccgtaaaac ctcgtccttc agggcgggga tataaggcgc
    21061 ggttttccac ctgaccaggt gtttgctgtt gttcaatgta ctggcggatg acggatattg
    21121 gcgcaccttc gcagctactg gcaaaatagc caggactcca caaaacgcct ttgtagtaat
    21181 acctgactgc aatatctggt cgatctcgtc gcagtaacct attcgatatg cctttgaggc
    21241 tgtttaccag actggatatc gccagtttgg gaggatagtt aattaacaaa tggacgtgat
    21301 ctggttcgcc atccatttca accagttcag ctgcaaaatc agcacataca tttgaaaagt
    21361 aagtgcgtag tttttctgtc gtgtcatggt caaaaatctg gcgtcggtat ttggtgacaa
    21421 agaccaggtg aacatgcatc aggaaaacac aatgccttcc acgccggata tcggtttctt
    21481 ttttcacaga ccaaagtatc atttgacctg tgaaacgatt acaggcattt aaattccagt
    21541 taagacccgg tggtcaacag gagcgtgaaa tgaggcgctt cgcaggcgca tgtcgtttcg
    21601 ttttcaatcg tgctctggcg cttcagaatg agaatcatga ggccggtaat aaatacatcc
    21661 cttacgggaa aatggcttcc tggctggttg agtggaaaaa cgccactgaa acgcaatggc
    21721 ttaaagattc gccctcacag ccattgcaac agtcactgaa agaccttgag cgggcttaca
    21781 aaaacttctt ccagaagcgg gctgcttttc cccgattcaa aaagcgggga cagaatgctg
    21841 cattccgcta cccgcaggac gttaagctcg atcaggaaaa cagccgtatt tttctgccga
    21901 aacttggctg gatgcgctac cggaatagcc gtcaggtcac gggagttgtg aaaaatgtca
    21961 ctgtcagcca gtcctgcggt acgtggtaca tcagtattca gacagaaagt gaagtatcaa
    22021 ctccggttca cccttcagca tcaatgatcg ggctggatgc tggcgtggct aaactcgcca
    22081 cgctgtcaga tggcacagtc tttgagcctg taaacagttt tcaaaaaaac cagaagacgc
    22141 tggcgagact tcagcgacag ttaagccgga aggtcaaatt cagcaacaac tggcagaagc
    22201 agaaacgcaa aatacagcga ctgcattcct gtatcgcaaa tatccgcagg gactaccttc
    22261 acaaagtcac aacgaccgtc agcaaaaacc acgcaatgat agtcattgag gatttgaagg
    22321 tcagcaacat gtcaaagtca gcagcgggta cggtcagcca gccggggcgc aatgtccggg
    22381 caaaatcagg tttaaaccgt tcgatactgg atcagggctg gtatgaaatg cgccgccagc
    22441 ttgagtacaa gcagctctgg agtggcggtc aggtgcttgc tgttccgcca gcgtacacaa
    22501 gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa ccgcctgtca caaagtaaat
    22561 tcagatgcca ggtatgtgga tatacagcga acgccg
//
LOCUS       DAJTYM010000064        22496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTYM010000064 DAJTYM010000000
VERSION     DAJTYM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO575_002529"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO575_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6665447.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO575_002530"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO575_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6665448.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO575_002531"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO575_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6665449.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO575_002532"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO575_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6665450.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO575_002533"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO575_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6665451.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO575_002534"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO575_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6665452.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO575_002535"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO575_002535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6665453.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO575_002536"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO575_002536"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6665454.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO575_002537"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO575_002537"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6665455.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO575_002538"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO575_002538"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6665456.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO575_002539"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO575_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6665457.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO575_002540"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO575_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6665458.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO575_002541"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO575_002541"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6665459.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO575_002542"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO575_002542"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6665460.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO575_002543"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO575_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6665461.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO575_002544"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO575_002544"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6665462.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO575_002545"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO575_002545"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6665463.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO575_002546"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO575_002546"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6665464.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO575_002547"
     CDS             21622..22212
                     /locus_tag="OO575_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6665465.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..>22496
                     /locus_tag="OO575_002548"
     CDS             22399..>22496
                     /locus_tag="OO575_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR6665466.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAIT"
BASE COUNT         5361 a         5806 c         6152 g         5177 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattac
//
LOCUS       DAJTYM010000065        22268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTYM010000065 DAJTYM010000000
VERSION     DAJTYM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1015)
                     /locus_tag="OO575_002549"
     CDS             complement(<1..1015)
                     /locus_tag="OO575_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6665467.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAP"
     gene            complement(1117..2097)
                     /locus_tag="OO575_002550"
     CDS             complement(1117..2097)
                     /locus_tag="OO575_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6665468.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2105..2815)
                     /locus_tag="OO575_002551"
     CDS             complement(2105..2815)
                     /locus_tag="OO575_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6665469.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3032..4855)
                     /gene="typA"
                     /locus_tag="OO575_002552"
     CDS             complement(3032..4855)
                     /gene="typA"
                     /locus_tag="OO575_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6665470.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5228..6637
                     /gene="glnA"
                     /locus_tag="OO575_002553"
     CDS             5228..6637
                     /gene="glnA"
                     /locus_tag="OO575_002553"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6665471.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6810..7859
                     /gene="glnL"
                     /locus_tag="OO575_002554"
     CDS             6810..7859
                     /gene="glnL"
                     /locus_tag="OO575_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6665472.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7871..9280
                     /gene="glnG"
                     /locus_tag="OO575_002555"
     CDS             7871..9280
                     /gene="glnG"
                     /locus_tag="OO575_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6665473.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9392..9502
                     /locus_tag="OO575_002556"
     CDS             9392..9502
                     /locus_tag="OO575_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6665474.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9731..11104)
                     /gene="hemN"
                     /locus_tag="OO575_002557"
     CDS             complement(9731..11104)
                     /gene="hemN"
                     /locus_tag="OO575_002557"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6665475.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11293..11802)
                     /gene="yihI"
                     /locus_tag="OO575_002558"
     CDS             complement(11293..11802)
                     /gene="yihI"
                     /locus_tag="OO575_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6665476.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12384..13016
                     /locus_tag="OO575_002559"
     CDS             12384..13016
                     /locus_tag="OO575_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6665477.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13398..16184)
                     /gene="polA"
                     /locus_tag="OO575_002560"
     CDS             complement(13398..16184)
                     /gene="polA"
                     /locus_tag="OO575_002560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6665478.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16548..17480
                     /locus_tag="OO575_002561"
     CDS             16548..17480
                     /locus_tag="OO575_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6665479.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17521..18951)
                     /locus_tag="OO575_002562"
     CDS             complement(17521..18951)
                     /locus_tag="OO575_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6665480.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19106..19732)
                     /gene="dsbA"
                     /locus_tag="OO575_002563"
     CDS             complement(19106..19732)
                     /gene="dsbA"
                     /locus_tag="OO575_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6665481.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19749..20735)
                     /gene="srkA"
                     /locus_tag="OO575_002564"
     CDS             complement(19749..20735)
                     /gene="srkA"
                     /locus_tag="OO575_002564"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6665482.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20812..21081)
                     /locus_tag="OO575_002565"
     CDS             complement(20812..21081)
                     /locus_tag="OO575_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6665483.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21151..21735
                     /gene="mobA"
                     /locus_tag="OO575_002566"
     CDS             21151..21735
                     /gene="mobA"
                     /locus_tag="OO575_002566"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6665484.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21717..22244
                     /gene="mobB"
                     /locus_tag="OO575_002567"
     CDS             21717..22244
                     /gene="mobB"
                     /locus_tag="OO575_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6665485.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5514 a         5579 c         5330 g         5845 t
ORIGIN      
        1 taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc agcgtacatg
       61 ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt acgagcgcag
      121 cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg atcttatctg
      181 aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta atgatgccgt
      241 aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga atgaagaatg
      301 tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc cagatggttt
      361 tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct tttttcacct
      421 caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg cctacggcaa
      481 tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca ctgccaaacc
      541 aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca cgccctgttt
      601 cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt accgacttga
      661 gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag acaaagagga
      721 tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc gcaatgagtg
      781 agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac agaaaaaagc
      841 ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca ttggtcaaat
      901 ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat ttataaattg
      961 taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg agcataacat
     1021 cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac cattcatatg
     1081 gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa gatggtttgc
     1141 ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa ttgcatcttc
     1201 tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg tttcactcat
     1261 ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg ccgacgcata
     1321 gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat cgccaaggca
     1381 gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa tacaggcttt
     1441 atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga cctgggtaat
     1501 atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga tcatgttagc
     1561 aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg acaccagctc
     1621 atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt cctgatacgt
     1681 ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca aattaccttc
     1741 ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg cgacggcttc
     1801 gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt tcagacggcg
     1861 ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag aaaaacgctc
     1921 gccatccaga acatggattt tgacgccacg tagattattt tcactggcga tatcaagtaa
     1981 atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag catgtaaata
     2041 aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg tcaccataaa
     2101 acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca atatctatct
     2161 ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct ggcatacccg
     2221 cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg aaaatcaatt
     2281 tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat gagtggtttt
     2341 caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg tgatagaaaa
     2401 ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt gatggcgtca
     2461 ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc tcggtaaacc
     2521 cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc gacagctccg
     2581 gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta cggaagattt
     2641 tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta atgcccagca
     2701 gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg ccatctttca
     2761 accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc gccattgttt
     2821 aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa gaaagcaatt
     2881 aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta atcacagact
     2941 tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa cctgccatcg
     3001 ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg cggttggcgc
     3061 ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc ggagtcactt
     3121 ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg cggataggcg
     3181 gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt ttcttaccgg
     3241 tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc tgaccttcgt
     3301 aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg aacagcgcga
     3361 acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc tgacccactt
     3421 cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca gtaccggaag
     3481 tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc acgtagtcga
     3541 gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta cgttcgccca
     3601 gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg ttttcatacg
     3661 gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat accgccagtt
     3721 cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca ccacgaccag
     3781 aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg ttgtgtacca
     3841 gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct tctttaccgc
     3901 agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca acggagagtg
     3961 ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc agttcgccca
     4021 ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata cgttccagac
     4081 cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg ctatcgatga
     4141 tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg ccgataacgc
     4201 caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg tcaaggtcaa
     4261 cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg tcttccgcca
     4321 tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg atcgggaagt
     4381 ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga tccacaaccc
     4441 aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc ttcaggccgt
     4501 aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca aatgcgtcaa
     4561 ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca ccgaagtcgg
     4621 cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata gcggtgtttt
     4681 tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc acgcgctctt
     4741 gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct accagggtgg
     4801 ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg atcacaactt
     4861 tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact acaaaacagg
     4921 atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc tttcacggag
     4981 cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt tccattgaag
     5041 cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg ttaagcatga
     5101 taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct tttttacggc
     5161 gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa tccaggagag
     5221 ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag tgaagtttgt
     5281 tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc ctgctcatca
     5341 ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga ttggcggctg
     5401 gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg cagtgattga
     5461 cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac ctggcaccct
     5521 gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt acctgcgttc
     5581 cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc tgttcgatga
     5641 catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata tcgaaggcgc
     5701 atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg cagtgaaagg
     5761 cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg aaatgtgtct
     5821 ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag cgactgctgg
     5881 tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg aaattcagat
     5941 ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga cctttatgcc
     6001 aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc tgtctaaaaa
     6061 cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg cgctgtacta
     6121 cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc cgaccaccaa
     6181 ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt actctgcgcg
     6241 taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac gtcgtatcga
     6301 agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc tgctgatggc
     6361 cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca aaaacctgta
     6421 tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc tggaagaagc
     6481 actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt tcacggatga
     6541 agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc gtatgactcc
     6601 gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt actacccggc
     6661 gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca tgggcttttt
     6721 tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata atgcactaaa
     6781 atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc gatgctgggc
     6841 agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg gcgatccatt
     6901 acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg tttggtacgc
     6961 cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa gaaagtctgg
     7021 aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt cgctcgcata
     7081 tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag atggcaccga
     7141 tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa gtagctgccc
     7201 gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt ttacgtggcg
     7261 cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat accaaagtga
     7321 ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg ccgcagctgc
     7381 ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta acgctggtgt
     7441 cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc ctgccggaac
     7501 tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc aatgcgctac
     7561 aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg tttcaactga
     7621 ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat aacgggccgg
     7681 gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc cgcgaaggtg
     7741 gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca ggcaaaattg
     7801 aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc aggaaataaa
     7861 ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt ccatccgttg
     7921 ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg agaacggcgc
     7981 ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag atatccgtat
     8041 gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc caatgcttcc
     8101 ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct atcaacaagg
     8161 ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc tggttgagcg
     8221 cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta acggcccaac
     8281 gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta tcggtcggct
     8341 ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta aagaactggt
     8401 cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg cgctgaatat
     8461 ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga aaggcgcgtt
     8521 tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg gcacgttatt
     8581 tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc
     8641 agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg tgcggattat
     8701 cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc gtgaggatct
     8761 gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac gtcgggaaga
     8821 tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg gcgtagaagc
     8881 gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc caggcaacgt
     8941 gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc aggaagtgtt
     9001 gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta cttcgcaaat
     9061 gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc tgcgttccgg
     9121 tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac tgacgaccgc
     9181 gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct ggggccgcaa
     9241 cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt gtgtgtaaag
     9301 attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat aagttcagta
     9361 tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta atctggtatc
     9421 gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc tgtgtgccgc
     9481 gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga aagtcgtagg
     9541 cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg atgcggcgta
     9601 aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc cagcgcatca
     9661 ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta aacgaagcgc
     9721 cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg cgcagatagg
     9781 tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc acctgtattc
     9841 ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc ttgagatctt
     9901 ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg taatccagac
     9961 ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca cgcgttagcg
    10021 caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac tgcttcaact
    10081 ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg cccatcccca
    10141 gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc acgccttcac
    10201 gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg ataaactgat
    10261 agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt tgctgcggac
    10321 tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc agatgcgcgt
    10381 agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg gtaaaggcga
    10441 aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg gaggtaaaac
    10501 caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc tcgcggttaa
    10561 ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga ttaaagcctt
    10621 cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact tcgatcgaaa
    10681 tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga cggctgattt
    10741 gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg acgtgacgcc
    10801 cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga tactgatcgg
    10861 ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa agcttatggc
    10921 agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc acggcttgta
    10981 aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac gaggtgtatc
    11041 gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt tgctgtacag
    11101 acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg gatgcccgtt
    11161 tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct gtagtttaac
    11221 gaataaccac accagataac atataaccgg aagggttatc agcagaacgg gaaggcccac
    11281 ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct tctcgtcttc
    11341 ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact catcaatacg
    11401 atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc ctgcttccag
    11461 acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact ccgcctgcgg
    11521 tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt cagtcactcc
    11581 caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct ggcctttgct
    11641 gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac gacgtttttt
    11701 ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc gacgcgcttt
    11761 tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc gtcttttagc
    11821 cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa gaaaaaaggc
    11881 gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc ttacaatcct
    11941 tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt tgattccatt
    12001 tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct tcgcctcctg
    12061 gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc tatgggtgct
    12121 actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca cattttcaga
    12181 gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat cgcagaattt
    12241 tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg gcttttactt
    12301 acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc gccacaaagc
    12361 tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg cattttgtga
    12421 tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg gcttttgcag
    12481 gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag aaaagcctgg
    12541 ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa gtggctgacg
    12601 gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa gagatgaagc
    12661 gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg caaggtctgg
    12721 tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg attgagtggg
    12781 cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa ctggcaagcg
    12841 gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt aacggtgatg
    12901 tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg aggcagaaac
    12961 tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc gaataatttt
    13021 cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa tagattacaa
    13081 ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt ctttcgccca
    13141 ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc aaatccgatt
    13201 acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac gcgactaact
    13261 ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag ttgttgcata
    13321 ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta cgaaaaaagg
    13381 gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc cacttccacc
    13441 agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg cttcgcgacg
    13501 gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg catgatcata
    13561 cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc ccgtttgata
    13621 atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc cgctgcacga
    13681 cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc cagcgtttca
    13741 acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag cacgccaggg
    13801 tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat gttcaattgc
    13861 cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc tttcgcgcta
    13921 cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc cgccgttgcc
    13981 cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg cgagagatgc
    14041 gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata atcctctggc
    14101 gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat gttttgcagg
    14161 ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata agaggtatgc
    14221 acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt cgacttcagc
    14281 ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag cgccagttct
    14341 tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag cggtttaata
    14401 ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt aaattcttca
    14461 cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag ctcttcagaa
    14521 tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg tgaaagcacc
    14581 ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg tttctgcaga
    14641 tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc ggcgtaacgt
    14701 ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt acccgcaatc
    14761 tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc catatcgtga
    14821 cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc aatcccacgc
    14881 agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg cccgaccttc
    14941 agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc gtgagagatt
    15001 tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac gcctggctcg
    15061 atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc ggtttcggta
    15121 tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag tgtttcttca
    15181 tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc tggcgcttcg
    15241 tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc ctgtaaccat
    15301 ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa cagccccaac
    15361 aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc cagttcaaca
    15421 tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt ttgctcgagc
    15481 ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc tggctcggca
    15541 tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt ttcaccgacg
    15601 cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa gtcgatgatc
    15661 aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat ggtattcgtc
    15721 atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt atcgccagtg
    15781 ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc gataacgtcg
    15841 tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac catcgcgtgc
    15901 agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga tttgtaatgt
    15961 tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc tgcatgcgtc
    16021 ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat cgcaccggtc
    16081 ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata aagataagat
    16141 gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc cgtgcctgtt
    16201 tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt ttcaccgctt
    16261 ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt ggataacttt
    16321 gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag catgatgttt
    16381 gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga taatgggatt
    16441 ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg ttatttgggt
    16501 cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg gctaaccttt
    16561 tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg agtatcgtat
    16621 tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg attgtaaaac
    16681 tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat tttatgatgt
    16741 attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg caatgggaag
    16801 ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt aaccaccgta
    16861 gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg atgaataaat
    16921 atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc tgggcgctgg
    16981 atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg gagcgacgtg
    17041 gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat cccaccacca
    17101 ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa acccactcca
    17161 cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta aatgtactgg
    17221 gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat aaccgtcagc
    17281 cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg gatttacagc
    17341 ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag cgccattttc
    17401 agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct ctcatgtcat
    17461 cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg agtgagtatt
    17521 ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct cctgtccgtt
    17581 aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt tgtcaatcgt
    17641 gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat cagcctgctt
    17701 ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg tttctgtgac
    17761 gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt ttgtggatga
    17821 tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt cgccgagtgc
    17881 gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata acggtaaata
    17941 ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg ctaactcagg
    18001 gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt ggcgcactgc
    18061 tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt gctgattccc
    18121 ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt tgtctttaac
    18181 gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt ccccgtgatt
    18241 gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt tcttttcttc
    18301 taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca ttggtgataa
    18361 ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg aactaccatc
    18421 accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg caggggcaac
    18481 atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt ccacgggcga
    18541 tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta agttcacttt
    18601 tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg gcaacggacc
    18661 gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga gatcgggtgc
    18721 accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt tgtcaaagtt
    18781 tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat cgttaaaatc
    18841 tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg tgccaccacc
    18901 ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca tataaatatt
    18961 ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt ccagcggcag
    19021 gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg tgaatattca
    19081 cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc accgtatcag
    19141 catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga ttcagctgat
    19201 atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct gcggcttttt
    19261 cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg tactcttcac
    19321 ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga atggtctggg
    19381 ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc agcgccatcg
    19441 ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag ttgacgtggt
    19501 atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa atatgcagaa
    19561 cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc acttgcggcg
    19621 cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac tgcgccgccg
    19681 atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc atgatctact
    19741 ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac ttttgcctgt
    19801 tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca cgggaaattt
    19861 ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata aaccaaacgc
    19921 atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct aaattcttca
    19981 taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc attgagcaac
    20041 atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac aaacattgga
    20101 ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag aacggtgaaa
    20161 tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt cagaaatgcc
    20221 gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg ctcaatgaga
    20281 tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc cgtctggtgc
    20341 atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc ttcgaactgg
    20401 cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa taaagtctga
    20461 ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg caacgcaaat
    20521 tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa tttgacgaca
    20581 aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct gttaagcggg
    20641 gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat ggtatccggg
    20701 tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa acattacgaa
    20761 tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct tttactcttt
    20821 aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat ctttctgcaa
    20881 acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga tcagaatgtc
    20941 atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt tctgcaaaaa
    21001 ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt caataacttc
    21061 attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca cgttagcagg
    21121 gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat aacaggcgtt
    21181 gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt gctggactta
    21241 aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct ctctcacgtc
    21301 gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa agtgattgaa
    21361 gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat gcagcaggaa
    21421 gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca tgatttagcc
    21481 gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga cggtgaacgc
    21541 gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact ggaatatctg
    21601 caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca tgcggttgat
    21661 ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct tgcccgatgg
    21721 caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc ggaaaaacta
    21781 cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg gggctgatta
    21841 agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat gagctgcgca
    21901 aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg atgacggaaa
    21961 caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat acctcaaagc
    22021 tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt gtgctgtttc
    22081 gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt attgctgtag
    22141 ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat gttgaggggc
    22201 tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga gtaggctgga
    22261 tcagacgc
//
LOCUS       DAJTYM010000066        21714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTYM010000066 DAJTYM010000000
VERSION     DAJTYM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..2237
                     /locus_tag="OO575_002568"
     CDS             <1..2237
                     /locus_tag="OO575_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR6665486.1"
                     /translation="QVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPE
                     RWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSS
                     GQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLK
                     GFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADG
                     SVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWY
                     GGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQ
                     LGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKL
                     QVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQ
                     PDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLL
                     DDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSL
                     VAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIA
                     LDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATL
                     DGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMD
                     AICR"
     gene            2228..3211
                     /locus_tag="OO575_002569"
     CDS             2228..3211
                     /locus_tag="OO575_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6665487.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3309..3849
                     /locus_tag="OO575_002570"
                     /pseudo
     CDS             3309..3849
                     /locus_tag="OO575_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3857..4372
                     /locus_tag="OO575_002571"
     CDS             3857..4372
                     /locus_tag="OO575_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6665488.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4338..5075
                     /locus_tag="OO575_002572"
     CDS             4338..5075
                     /locus_tag="OO575_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6665489.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5186..6196
                     /gene="pyrD"
                     /locus_tag="OO575_002573"
     CDS             5186..6196
                     /gene="pyrD"
                     /locus_tag="OO575_002573"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6665490.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6370..6912
                     /gene="zapC"
                     /locus_tag="OO575_002574"
     CDS             6370..6912
                     /gene="zapC"
                     /locus_tag="OO575_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6665491.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(6909..8018)
                     /gene="ycbX"
                     /locus_tag="OO575_002575"
     CDS             complement(6909..8018)
                     /gene="ycbX"
                     /locus_tag="OO575_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6665492.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8262..10370
                     /gene="rlmKL"
                     /locus_tag="OO575_002576"
     CDS             8262..10370
                     /gene="rlmKL"
                     /locus_tag="OO575_002576"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6665493.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10382..12289
                     /locus_tag="OO575_002577"
     CDS             10382..12289
                     /locus_tag="OO575_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6665494.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12419..13672
                     /gene="pqiA"
                     /locus_tag="OO575_002578"
     CDS             12419..13672
                     /gene="pqiA"
                     /locus_tag="OO575_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6665495.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            13677..15317
                     /gene="pqiB"
                     /locus_tag="OO575_002579"
     CDS             13677..15317
                     /gene="pqiB"
                     /locus_tag="OO575_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6665496.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15314..15877
                     /gene="pqiC"
                     /locus_tag="OO575_002580"
     CDS             15314..15877
                     /gene="pqiC"
                     /locus_tag="OO575_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6665497.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16133..16300
                     /gene="rmf"
                     /locus_tag="OO575_002581"
     CDS             16133..16300
                     /gene="rmf"
                     /locus_tag="OO575_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6665498.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16370..16888)
                     /gene="fabA"
                     /locus_tag="OO575_002582"
     CDS             complement(16370..16888)
                     /gene="fabA"
                     /locus_tag="OO575_002582"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6665499.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(16957..18717)
                     /locus_tag="OO575_002583"
     CDS             complement(16957..18717)
                     /locus_tag="OO575_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6665500.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            18902..19354
                     /gene="matP"
                     /locus_tag="OO575_002584"
     CDS             18902..19354
                     /gene="matP"
                     /locus_tag="OO575_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6665501.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19430..20476)
                     /gene="ompA"
                     /locus_tag="OO575_002585"
     CDS             complement(19430..20476)
                     /gene="ompA"
                     /locus_tag="OO575_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6665502.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(20832..21341)
                     /gene="sulA"
                     /locus_tag="OO575_002586"
     CDS             complement(20832..21341)
                     /gene="sulA"
                     /locus_tag="OO575_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6665503.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21560..>21714
                     /locus_tag="OO575_002587"
     CDS             21560..>21714
                     /locus_tag="OO575_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCR6665504.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRA"
BASE COUNT         5531 a         5113 c         5692 g         5378 t
ORIGIN      
        1 aacaagtgtg tgtgccattg gcggagatta ttccggatgc cagcgtaacg tttaatgtga
       61 ataaactccg tctggaaatt tcagtaccgc aaatcgccat caaaagtaat gcccgtggtt
      121 atgtcccccc tgaacgttgg gatgaaggga tcaacgcgct attactggga tattcgttta
      181 gcggggctaa cagtattcat agcagcgcag acagtgattc tggcgacagc tattttctga
      241 atttaaacag tggcgttaat ttaggcccat ggagattacg caacaattca acatggagtc
      301 gcagtagtgg ccaaaccgca gaatggaaga atctcagcag ctatttgcag cgggcggtta
      361 ttccactaaa aggcgaactg accgtaggtg atgattatac tgcaggcgat tttttcgata
      421 gtgtcagctt tcgtggtgtg cagctggcgt cagatgacaa catgctgcca gacagcctga
      481 aagggtttgc gcctgtggtg cgtggtatcg ccaaaagcaa tgcccagata acgattaagc
      541 aaaatggtta caccatttac caaacttatg tatcgcctgg tgcttttgaa attagtgatc
      601 tctattccac gtcgtcgagc ggtgatttgt tagttgaaat caaagaagcg gacggcagcg
      661 tcaatagcta cagcgtaccg ttttccagcg tgccattact ccagcgtcag gggcgaatca
      721 aatacgcggt gacactggcg aaatacagaa ccaatagtaa tgaacagcag gagagcaaat
      781 ttgcccagac cacgttgcag tggggcggac cgtggggaac gacatggtat ggtggtggac
      841 aatatgctga atattaccgt gccgccatgt ttggtctggg atttaacctt ggcgatttcg
      901 gagcaatttc gttcgatgcg acccaggcga agagtacgct ggcagaccaa agcgaacata
      961 aaggtcagtc atatcgtttt ctgtatgcca aaacgctcaa ccaattgggc actaattttc
     1021 aattgatggg ctatcgctat tcgacgtcgg gtttctacac cctttccgac accatgtata
     1081 aacatatgga tggctacgaa tttaatgacg gtgatgatga agatacgccg atgtggtcgc
     1141 gttattacaa tttgttttac accaaacgtg gcaaactgca ggtcaatatc tcccagcaat
     1201 taggcgagta cggttcgttt tatttaagtg gtagccagca aacttactgg cataccgatc
     1261 aacaggatcg gctattacag tttggctaca acacgcaaat taaagatctc tcgctggggg
     1321 tttcctggaa ctacagtaag tcccgtggtc aacctgatgc tgatcaggtg tttgcactaa
     1381 atttttccct gccgctcaat ctgttgctcc ccagaagtaa tgatagctat accaggaaaa
     1441 aaaattacgc ctggatgacc tctaacacca gtatcgataa cgaagggcac attacacaaa
     1501 acctgggttt aacggagaca ctactcgatg acggtaacct gagctacagc gtgcaacagg
     1561 gatataacag cgaggggaaa acggctaatg gtagcgccag catggactac aaaggggcgt
     1621 ttgcagatgc ccgagtgggc tacaactaca gcgataacgg cagtcaacaa caactgaact
     1681 acgctctttc aggcagttta gttgcccatt cacagggcat taccctgggg caatcgctgg
     1741 gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa tactcgtgtg gcgaacagca
     1801 ccgggctgaa aactgactgg cgcggatata ccgttgtccc ctatgccact tcttatcggg
     1861 aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa cgtggatctt gaaaatgcag
     1921 tagtcaacgt ggttcccacc aaaggggcgt tggttctggc ggagttcaat gcccatgcgg
     1981 gagcaagggt attaatgaaa acatcaaagc agggtatacc gctgcgtttt ggcgcgatag
     2041 cgacgctgga cggcatacag actaatagcg gcattattga tgatgatggc tcgctctata
     2101 tgtccggttt gcccgctcag ggagcgataa ctgtacgctg gggcgaagct cccgatcaaa
     2161 tttgtcatat cagttaccag cttaccgaac aacaaattaa ctctgcgatt acgcgaatgg
     2221 atgccatatg cagataatct ttggagaaaa atgcgtgtta ttactacgac tattttttgc
     2281 cgccgtctta atgctatggt gcgctcaaac tgctgcttat atcgggcagt gtcataccac
     2341 tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa actctggatg aagaagaaaa
     2401 tacggctgga gtagttaaag acaaatttta tcagtggaac gaatcgaatg attattatgt
     2461 ttcctgtgat tgcgataaag acaatgtcag aagtggccga tgggcattcg ccgcggattc
     2521 accgttagtc tatttaggcg acaactggta caaaattaat gactatcttg ccgccaaagt
     2581 tttattgcag gttaaaggca gttctcctac tgcggttcct ttcgaaaacg tgggcacagg
     2641 ggcagataca cgatggcata tttgcgatcc cggcggtcaa cgtttaggtg gccagggggc
     2701 tagcggtaat agcggtagct tttccctgaa aatattgcag ccgttcgttg gttcggtcgt
     2761 cattcctcct atggcgctgg cgcgattatt tgaatgctac aacatacccg caggtgattc
     2821 ctgcacgact actggtacac cggttttagt gtattacctg tctggtacga tcaattcact
     2881 tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt gatctgggcg acgtatttgc
     2941 ggctaacttt cgtgttgtag ggcataagcc tcttggggcc agaacggcag aacttgcaat
     3001 tccggtcagg tgtaacacgg gaaacgcagg gttagttaac gtcaatctga gtctgacggc
     3061 aacctcagac cccagctatc cccaggcgat taagacgtca cgtcctggcg tgggcgtggt
     3121 ggtgaccgat agccagcaca acattatttc ccctgctggt gggacactac cgctctctat
     3181 tcctgatgat gcagacagta tccgcgaatg aatgtctatc cagtcagcac gacaagcgta
     3241 ccaccagaaa ccgggcgatt tgaagccacg gcaacggtga gaataaattt tgattaacgc
     3301 gtgaacgtat gaagaaaaaa acgatatatc agtgcgttat tttgttcttt agccttctta
     3361 acatccatgt cgggatggct gggcctgaac aagtcagtat gcatatttat gggaatgtgg
     3421 tcgatcaggg ctgtgatgtc gccaccaaaa gtgcattaca aaatattcat attggtgatt
     3481 ttaatatcag tgattttcag gccgcgaata ccgtaagcac tgctgctgat ttgaatattg
     3541 atatcaccgg ttgtgccgct ggtattactg gcgcggacgt cctttttagc ggcgaggctg
     3601 acacccttgc gccgacactg ctcaaactaa ctgacacagg cggaagcggt ggtatggcaa
     3661 cggggattgc cgtgcattct tgatgcgcaa agtcagcaag aaatcccgct caatcaggtc
     3721 cagcctctta cgcccttaaa agccggggat aacacactca aatatcaact tcgttataag
     3781 tccacaaagg cgggagcaac gggcggtaat gcgacggcgg ttctctattt tgatctggtt
     3841 taccagtgaa ggagtgatgt tgaaacggat aatctggatt ctgttcttac tgggattaac
     3901 ggggggctgt gagctatttg cccatgatgg cacggtcaac attagcggat cgtttcgccg
     3961 taatacatgc gtgctggcac aggatagcaa gcaaatcaat gtgcagttag gcgatgtctc
     4021 gctgacacgt ttttttcatg ggaattatgg cccggagaaa tctttcatca ttaatttgca
     4081 ggattgcgga acagatgtca gcacggttga tgtcaccttt tcaggaaccc ccgacggagt
     4141 gcagagcgaa atgctgtcca ttgaaagtgg tacggatgca gccagtgggc tggcaattgc
     4201 gattctggat gatgcgaaga tattgattcc gcttaaccag gcgagtaaag actacagcct
     4261 acatagcggt aaggtgccat taacttttta tgcgcaattg cgacctgtca atagcgatgt
     4321 gcagtcgggt aaagtgaatg ccagcgcgac atttgtactt cattatgact aatacgtgga
     4381 acagattagc gctcttgatt ttcgccgttt tatcgctgct ggtggcgggt gaattgcagg
     4441 caggagtggt ggtcggcgga acgcgattta tctttccggc agacagagaa tcgatatcta
     4501 ttttactgac taataccagt caggaatctt ggcttattaa cagcaaaatc aatcgcccaa
     4561 cgcgttgggc cgggggggaa gcgtcgacag tgccagcact attactggcc gctccgccac
     4621 ttattctcct gaagcccggt actacaggca cgttgcgctt gctgagaacg gaaagcgaca
     4681 tcttgcctgt ggatcgcgaa acgctatttg agttaagcat tgccagcgtg ccatccggca
     4741 aagttgaaaa tcagagcgta aaagtggcga tgcgctcggt atttaaactg ttctggcgac
     4801 ccgaaggttt gccgggcgac ccgctggaag cttatcaaca attacgctgg acacggaatt
     4861 cgcagggtgt acaactcact aacccaacgc cttactacat taacctgatt caggtgagcg
     4921 taaatggtaa agcgttaagt aatgcgggag tcgtgccgcc taaaagccag cgtcagacaa
     4981 actggtgtca ggtaatagca ccgtgtcatg tcgcctggcg gacgataaat gattacggcg
     5041 ggttgagtgc aaagaaggag caaaatctgc cctgaaacag gttctgaaaa cgtttgcgtt
     5101 ttttttgccg caggtcaatt cccttttggt ccgaactcgc acataatacg cccccggttt
     5161 gcacaccggg aatccaggag agttcatgta ctaccccttc gttcgtaaag cccttttcca
     5221 gctcgatcca gagcgcgctc atgagtttac ttttcagcaa ttacgccgca ttaccggaac
     5281 accgtttgaa gcactggtgc ggcagaaagt gcctgcgaaa cctgttaact gcatgggcct
     5341 gacgtttaaa aatccgcttg gtctggcagc cggtcttgat aaagacgggg agtgcattga
     5401 tgcgttaggc gcgatgggat ttggatcgat cgagatcggt accgtcacgc cacgtccaca
     5461 gcctggtaat gacaagccgc gtctctttcg tctggtagat gccgaaggtt tgatcaaccg
     5521 tatgggcttt aataatcttg gcgttgataa cctcgtagag aacgtaaaaa aggcccatta
     5581 tgacggcgtc ttgggtatta atatcggcaa aaataaagat acgccagtgg agcagggcaa
     5641 agatgactat ctgatttgta tggaaaaaat ctatgcctat gcgggatata tcgccatcaa
     5701 tatttcatcg ccgaataccc caggattacg cacgctgcaa tatggtgaag cgctggatga
     5761 tctcttaacc gcgattaaaa ataagcaaaa tgatttgcaa gcgatgcacc ataaatatgt
     5821 gccgatcgca gtgaagatcg cgccggatct ttctgaagac gaattgatcc aggttgccga
     5881 tagtttagtt cgccataata ttgatggcgt tattgcaacc aataccactc tcgatcgttc
     5941 tctggttcag ggaatgaaaa attgcgatca aaccggtggc ttaagtggtc gtccgcttca
     6001 gttaaaaagc acagaaatta ttcgccgctt gtcactagaa ttaaacggtc gcttaccgat
     6061 catcggtgtt ggcggcatcg actcggttat cgctgcgcgt gaaaagattg ctgcgggtgc
     6121 ctcactggtg caaatttatt ctggttttat ttttaaaggt ccgccgctga ttaaagaaat
     6181 cgttacccat atctaattat ttcttcgaat tcctctttat aagataatca gggctttatt
     6241 ttcagacctg gttgttttat attcatcgtt gttgcttatt tagacatttt gtacttttat
     6301 tgttgaggtt attaagcgaa gcgacaatgg attgtgttgc tgcggtttta tagggtaggg
     6361 gagaggcaga tgcgaattaa accagacgat aactggcgtt ggtattacga tgaagagcac
     6421 gatcgaatga tgctcgattt agccaatggt atgctatttc gctcacgttt tgcgcgcaaa
     6481 atgttgactc cagatgcctt ttctcccgca ggcttttgtg ttgatgatgc cgcgctctat
     6541 ttctcttttg aagaaaagtg ccgcgatttt aatttatcta aagaccaaaa agccgaactg
     6601 gtgctgaatg cactggtagc aattcgttat ctcaaaccgc aaatgccgaa aagctggcat
     6661 tttgtctccc atggtgaaat gtgggtgccc atgccgggcg atgcggcctg cgtttggttg
     6721 agcgatactc acgagcaggt caatttgctg gtcgttgaat ctggcgaaaa tgccgcgcta
     6781 tgcctgctgg cacaaccctg cgttgttatt gcgggtcgcg cgatgcagtt gggcgacgcc
     6841 attaaaatca tgaacgacag gctgaaaccg caggtgaatg ttgacagctt cagcctcgaa
     6901 caggcagtct aacgcgtcag cttaagggca gtcttcggta cacagctaca gcaaagaatg
     6961 gtgccatcat cgcccattgc tgattttttc agcggtgtga cttcgccttc taaaagctga
     7021 acacggcaac ttccacaaat gcccgcgcgg caagaataag ggatacgaat tccctgattt
     7081 tctaattgct ccagcaacac ttgttggtta tttccacgaa atgcctgtcc ctgccagtca
     7141 atatctacat ttgcgtccgg ttgttgcgtg atgttggcgg tatcatcagc ggcagctgcg
     7201 ccgtaaattt ttgccggagc cgttgccaga atttccacct catcgccaac gcgaatcacg
     7261 ccgctattac gggcaattaa attctgacca aaatcgacat cgccgttatc ctgggcagtg
     7321 cggaaagatt gcaatgtttt taatggttcg cctgccggat gtttttgccc tttttctggg
     7381 ctgacggtgg tgaaaataca gcggctacaa ggtttaacca catcaaacac cacatcacca
     7441 atgcgaatca ctttccagct atcttcttcc caggctgacg cgccggaaac caccagattg
     7501 gggcggaatt gctccatttt tacactggcc ggacaacgtt gttggagatc acgtaacgag
     7561 gcttcgttag caagaaggta aggatagcca tcagcaaatg acagaggtac agtgttgtgg
     7621 cgtttcacgc gccgggtcat ttgtggcccc acccagcgta attgcacttc gcgggagaaa
     7681 aatccactta gccatttgtt gatcgcgtct ggcgcaattc gcgcggtaaa atgtgtgccc
     7741 caaacttcgg ttggtgcgtc ttgtgtggcg aaatcagcaa aacgaacata tgcgctactg
     7801 ccatctggtg cggtgagatg caagccatca tgcacaggtg aaggagtaaa ccgtaccatc
     7861 tggggaaact ggcgagcggt aataaacgta ccgtcaggtt ccgtgatcat aaagatgcga
     7921 tcgaaggcca gaccactgac atcagccaga gcatgtgtaa gaccaatgcc gcgcatcgat
     7981 ttaacaggat gaataaaaag ccgggttaat gtcgccacgg tgcggtcctc aaatgaaaat
     8041 aagccctcaa ctttatgaca tgacgcgctt attagctata atgcgcaaca attttcttag
     8101 cgcctgtccc gttaggctat tttatttgtc agtttggccc tgggttgtgc tcgaaatcct
     8161 cacgtacttg tgtacgctcc ggtttctccg cgctctccat atccagactg actgcaacaa
     8221 ttacgcctac tgcggtaggc tcttagagta aaagtgacga tatgaattct ctgtttgcca
     8281 gtacggcccg tgggctggaa gagctgttaa aaactgaact ggaaaatctg ggggccgttg
     8341 aatgccaggt ggttcagggt ggggtccatt tcaagggcga cacacggctt gtttaccaga
     8401 gcctgatgtg gagccgcctg gcctcgcgta ttatgttgcc gctgggcgag tgtaaggttt
     8461 acagcgattt agacctctat ctcggtgttc aggcgatcaa ctggacagag atgtttaatc
     8521 ctggcgcgac cttcgctgtc cacttcagtg gtttgaatga caccatccgc aacagtcagt
     8581 acggcgcgat gaaagtgaaa gacgcgatcg tcgatgcttt cacacggaaa aatctgccgc
     8641 gtccaaatgt tgatcgcgat gcgccggata tccgcgttaa cgtctggctg cataaagaaa
     8701 ccgccagtat cgctcttgat ctgagtggtg atggtttaca tctgcgtggc tatcgcgatc
     8761 gtgctggtat tgcgccgatc aaagaaaccc tggcagccgc tattgtgatg cgatccggct
     8821 ggcagtcagg aacaccgctg ctcgatccga tgtgtggttc cggtacgttg ctgattgaag
     8881 cggcgatgct ggcgaccgat cgcgcaccag gcttgcaccg tgggcgttgg ggctttagcg
     8941 gctgggcgca gcatgatgaa gctatctggc aggaagtgaa agcggaagcg caaactcgcg
     9001 cccgtaaagg cctagctgag tatagctctc atttttacgg ttcggacagc gacgcacggg
     9061 tgattcaacg tgcgcgcact aacgcccgtc ttgcggggat tggtgaactg atcacctttg
     9121 aggtgaaaga tgtcgcgcaa ctgaccaatc cgctgccgaa agggccgtac ggtacagtgt
     9181 tgagcaaccc gccatacggt gaacgtctgg acagcgaacc ggcgctgatt gcgctgcata
     9241 gtctgctggg ccgaatcatg aaaaaccagt tcggtggctg gaatctctct ttgtttagtg
     9301 cctcgccgga tctgctaagc tgcctgcagc tgcgtgcaga caaacagtac aaggcgaaaa
     9361 acggcccgct ggactgcgta cagaaaaatt accacgttgc cgaaagcaca ccagacagta
     9421 aaccggcaat ggtagcggaa gattacgcca accgcctgcg taagaacctc aaaaaattcg
     9481 agaagtgggc acgccaggaa gggattgaat gttaccgcct gtatgacgcc gatctgccgg
     9541 aatataacgt tgccgttgac cgttatgccg actgggtggt ggtgcaggag tatgcgccgc
     9601 caaaaactat tgatgcgcac aaagcgcgtc agcgtctgtt cgatattatc gctgcaacca
     9661 tttcggtact ggggattgcg ccaaacaaac tggtgctgaa aacccgtgaa cgccataagg
     9721 gcaaaaacca gtaccagaaa ctgggcgaga agggcgagtt tcttgaagtt accgaatata
     9781 acgctcactt gtgggtgaac ctgacggatt atctcgatac tggtctgttc ctcgatcacc
     9841 gtatcgcccg ccgtatgctc ggtcagatga gcaaaggcaa agatttcctc aacctgtttt
     9901 cttataccgg cagcgccacc gtgcatgcgg gattaggcgg tgcacgcagc accaccaccg
     9961 tggatatgtc gcgtacttat ctggaatggg cagaacgcaa cctgcgtctg aatggcctga
    10021 ctaggcgtgc gcatcgcctg attcaggccg attgcctggc gtggctgcgt gaggcaaatg
    10081 aacagttcga tctgatcttt atcgatccgc caaccttctc taactcaaaa cgaatggaag
    10141 atgcgtttga tgttcagcgc gatcatctgg cgctgatgaa agatttgaaa cgtctgctgc
    10201 gtgcaggtgg gacgatcatg ttctcgaaca acaaacgtgg cttccgtatg gatctcgacg
    10261 gcctggcaaa actgggactg aaagcacaag aaattacgca aaaaacgctc tcccaggatt
    10321 tcgcccgtaa ccgccagatc cacaactgct ggctgattac cgcagcctga aaggaatagt
    10381 aatgtcatta atcagtatgc atggcgcatg gctgtcgttc agcgacgcgc cgcttctcga
    10441 taacgcagaa ctgcatatcg aagataacga acgtgtttgt ctggtgggcc gcaacggcgc
    10501 aggaaaatcg acgttaatga aaatcctcaa ccgtgaacaa gggctggatg acggtcgcat
    10561 tatttatgag caagatttga ttgtagctcg tctgcaacag gatccgccgc gtaacgttga
    10621 gggtagcgtt tatgatttcg ttgccgaagg cattgaagaa caagcggaat atctgaaacg
    10681 ctatcacgat atttcgcgcc tggtgatgaa cgacccgagc gagaaaaatc tcaacgaact
    10741 ggcgaaggtt caggaacagc tggatcacca caacctgtgg cagctggaaa accgcatcaa
    10801 cgaagtgctg gcgcaactgg ggttagatcc taacgttgcg ctgtcgtcgc tttccggcgg
    10861 ctggttgcgt aaagcggcat taggacgcgc gctggtgagc aatccgcgcg tattgctgct
    10921 ggacgaaccg actaaccatc tggatatcga aaccatcgac tggctggagg ggttccttaa
    10981 aaccttcaat gggacgatta ttttcatctc ccacgaccgt tcgtttatcc gcaatatggc
    11041 gacgcgcatt gttgatctcg atcgcggcaa gctggtgacc tatccaggga attacgacca
    11101 gtacctgcag gaaaaagaag aagccctgcg cgtggaagaa ctacaaaatg ccgagttcga
    11161 tctcaaactg gcgcaggaag aagtgtggat tcgccagggg atcaaagcac gccgtacccg
    11221 taatgaaggc cgcgtacgcg ccctgaaagc gatgcgtcgc gaacgtggtg aacgtcgcga
    11281 agtgatgggt accgcaaaga tgcaggtgga agaggccagc cgctccggta agatcgtttt
    11341 cgaaatggaa gacgtttgct accaggttaa cggtaagcaa ctggtgaaag atttttctgc
    11401 ccaggttcta cgtggcgaca aaattgccct gattggtccg aatgggtgcg gcaaaaccac
    11461 gctgctgaaa ctgatgctcg gtcagcttca agcggacagc gggcgtattc acgttggcac
    11521 caaactggaa gtggcttatt tcgatcagca ccgcgcggaa ctggatcccg ataaaacggt
    11581 gatggataac cttgccgaag gtaagcaaga ggtgatggtt aatggcaagc cacgccacgt
    11641 attgggctat ttgcaggact ttctgttcca tccgaaacgg gcgatgacgc cagtacgtgc
    11701 gttttctggc ggtgagcgga accgcttgct gctggcgcgt ttgttcctca aaccaagcaa
    11761 cttattgatt cttgacgaac cgaccaacga tcttgatgtc gaaacgctgg aactgctgga
    11821 agaactgatc gacagctatc agggcacggt attgctggtt agccacgatc gtcagtttgt
    11881 cgataacacc gttacagaat gctggatctt cgaaggcggc ggtaaaattg gtcgttatgt
    11941 tggcggttat catgatgccc gtggtcagca agaacaatat gtggcactca aacagcctgc
    12001 ggtgaaaaaa agcgaagaag ccgccgcgcc aaaagcagaa actgtaaaac gcagcagtag
    12061 caaactaagc tataaattgc agcgcgaact ggagcagcta ccgcaattgc tcgaagatct
    12121 ggaggcgaag ctggaagccc tacagacgca agtggcggat gcttccttct tcagtcagcc
    12181 gcatgagcag acgcaaaaag tgcttgctga tatggctgct gcagagcagg agctggagca
    12241 agcctttgaa cgctgggagt atcttgaagc gttaaaaaat ggtggctgat cgcaaaatag
    12301 tcgatttaaa cagagacagc tgacaacggt aaaattgcct gatgaactgg gtttatcagg
    12361 catgaaaacc aacactttgt tagtcatctg taaaatagcg catcattaag gagtaccaat
    12421 gtgcgaacat catcatgccg cgaagcacat cctgtgctcg cagtgtgaca tgctggtggc
    12481 gttaccgcgc cttgagcatg gtcagaaagc ggcatgtccc cggtgtggca caacgttaac
    12541 cgtggcgtgg gatgcccctc ggcagcgtcc caccgcctat gcgttggctg cactgttcat
    12601 gctgttgctg tccaacttgt ttccttttgt gaatatgaac gttgcaggag ttaccagtga
    12661 aattacatta ctggaaattc ccggcgtgct tttttctgag gactacgcca gcctcggcac
    12721 ctttttcctg ttgtttgtgc aactggttcc cgcgttttgt ctgataacca ttctgttact
    12781 ggtgaatcgc gcggaattac cggtccgttt aaaagagcaa ctggcacggg tgctttttca
    12841 actcaaaacc tggggaatgg cggagatttt cctcgcgggt gtgctggtca gtttcgttaa
    12901 actgatggct tacggcagca ttggggtagg cagcagcttt ctcccctggt gtttattttg
    12961 tgtcctgcaa ctgcgcgctt ttcagtgcgt tgatcgtcgc tggttatggg acgacatcgc
    13021 cccgatgcca gaactgcgcc agccgctaaa accaggcgtc acggggatac gtcaggggct
    13081 gcgttcgtgc tcctgttgta cggcaatcct tcctgctgat gaacccgtgt gcccgcgttg
    13141 tagtaccaaa gggtacgttc ggcgtagaaa cagcctgcag tggacactcg cgctgcttgt
    13201 aacgtccatc atgctgtatc ttccggctaa tattttgccc atcatggtga cggatttatt
    13261 aggctcgaag atgccgtcga cgattctcgc tggggtcatt ctgttatgga gcgaaggctc
    13321 ttatcccgtc gctgcggtga tctttctggc cagtattatg gtgccaacgt taaagatgat
    13381 cgccatcgcg tggctgtgtt gggatgccaa agggcatggc aagcgcgaca gtgaaagaat
    13441 gcatttgatt tatgaagttg ttgagtttgt aggccgctgg tcgatgattg acgttttcgt
    13501 tatcgcggtg ctctcggcgc tggtgcgtat gggaggttta atgagtattt atccggcaat
    13561 gggtgcatta atgtttgctt tagtcgtcat aatgacaatg ttttctgcta tgacgtttga
    13621 cccgcgtttg tcgtgggatc gtcaacctga atcagagcat gaggagtcct gacagtatgg
    13681 aatctaataa tggggaagcc aaaatccaga aagtgaagaa ctggtctccc gtgtggatat
    13741 ttcctatcgt cacggcgctc attggggcct gggttctttt ttatcattac agccatcagg
    13801 gaccggaagt gaccctgatc accgcgaatg cggaaggaat tgaaggtggc aaaaccacca
    13861 ttaaaagccg tagcgttgac gtcggcgtgg ttgaaagcgc cacactggct gatgatttga
    13921 cgcacgttga aatcaaagcg cggctgaatt ccggtatgga aaaattgctg cataaagaca
    13981 ccgtcttttg ggtggtgaaa ccgcagattg gtcgcgaagg gattagcggc ctgggaacgc
    14041 tgctgtctgg agtttatatc gaactgcagc caggcgcgaa aggcagcaaa atggataaat
    14101 acgatttgct ggactcacca ccgttggccc cgcctgatgc gaaaggtatc cgtgtgattc
    14161 tcgatagcaa aaaagccggg cagctctcgc caggagatcc ggtgctgttc cgtggctatc
    14221 gggtaggttc ggttgaaacc agcaccttcg atacgcaaaa acgcaatatc agttatcaac
    14281 tgttcatcaa tgcaccttat gaccgactgg tgaccagcaa tgttcgcttc tggaaagata
    14341 gtggcattgc ggttgatctg acgtcagcgg ggatgcgtgt ggagatgggc tcattgacaa
    14401 cgctgcttag tggcggtgtt agctttgatg tgccggaagg tctggattta gggcagccag
    14461 tggcaccgaa aacagctttc gttttgtatg atgatcagaa gagcattcag gattcgttgt
    14521 acaccgatca cattgattat ctgatgttct ttaaagattc ggtacgcggt ctgcaaccgg
    14581 gagctccggt agaattccgg ggtattcgcc tgggtaccgt aagcaaagtg ccattctttg
    14641 cgccgaatat gcgtcagaca tttaacgatg attaccgtat tccggtactg attcgtatcg
    14701 agccagagcg gctgaaaatg cagcttggcg aaaatgcgga tgttgttgag caccttggcg
    14761 aattgttgaa acgtggttta cgcggatcgc tgaaaaccgg aaacctggtc actggtgcac
    14821 tgtatgttga tctcgatttc tatccaaata cgcctgcaat aaccggtatt cgtgaattta
    14881 atggttatca gattatcccg actgttagcg gcggcttggc gcaaatccag caacgactga
    14941 tggaagcgtt ggataagatc aacaaactgc cattgaatcc gatgattgaa caggcaacca
    15001 gtacgctttc tgaaagtcag cgcacaatga aaaacctgca aacgacgctg gatagcatga
    15061 acaagatcct cgccagccag tcgatgcagc agttgccgac ggatatgcag tcaacgttgc
    15121 gtgaattgaa tcgcagcatg cagggcttcc agcctggctc cgcagcctac aacaagatgg
    15181 tggcggatat gcagcgcctt gatcaggtgt tgcgagaact gcaaccggtg ctgaaaacgc
    15241 tcaatgagaa gagtaacgcg ctggtatttg aagcgaagga caaaaaagat ccagagccga
    15301 agagggcgaa acaatgaaaa agtggctagt gacgattgca gcactgtggc tggccggatg
    15361 cagctccggc gaaattaata aaaactatta ccagttacct gtggtgcaga gcggtacaca
    15421 aagtaccgct agccagggca atcgtctgtt atgggtagag caggtcactg ttcctgacta
    15481 tctggcgggg aatggtgtgg tttatcaaac cagtgatgtg aagtatgtga ttgccaacaa
    15541 caacttgtgg gccagcccgt tggatcaaca gttgcgcaac accctggttg ccaacctgag
    15601 tacgcaactg cccggctggg tggttgcctc ccagcctctg ggaagcgccc aggacacgct
    15661 caatgttacc gtaacggagt ttaacggtcg ctatgatggc aaggtcattg tcagtggtga
    15721 gtggctgttg aaccaccagg gacaactgat caaacgtccg ttccgtctgg aaggagtgca
    15781 aactcaggat ggttacgatg agatggttaa agtgctggcc ggtgtctgga gtcaggaagc
    15841 cgcttctatt gcacaagaga taaagcgtct accttaatta taaagatttg taaatataac
    15901 cgtctccggt atgttgcctg aggcggtttt tttgtttcta acgtgcggaa aaatttgttc
    15961 ctcttcacat tttttgtaca accgacatgc ccgtgtagct cacaaatatg acagtggcgt
    16021 gaattttgcg cattgacggc agttatgatt cgcggtattg cttaactgtg attgcacatt
    16081 tagtaatcac tgttttcttt tccaccagaa accagtatga gggaaacgag gcatgaagag
    16141 acaaaaacga gatcgcctgg aacgggcaca tcaacgtggt tatcaggccg gcatcgccgg
    16201 acgctcaaaa gaaatgtgtc cctatcagac gctgagtcaa aggtcacaat ggctgggagg
    16261 ctggcgagaa gccatggcgg acagggtagt aatggcctga ttctgtctct ttaaaaagaa
    16321 acctccgcat tgcggaggtt tcgccttttg atactctgtc tgatgataat cagaaggcag
    16381 acgtatcctg gaacagaccg actttcaggt cgctggcggt atagatcaga cgacaatcaa
    16441 ccagcacttc gccatctgcc agacccataa tcagacgacg attaacaatg cgtttaaagt
    16501 gaatacggta ggtcactttt ttcgctgtcg gcagtacctg accagtgaat ttcacttcgc
    16561 caacgcccag cgcgcggcct ttaccttcgc cgcccagcca gccgaggtag aaccctacca
    16621 gctgccacat tgcgtccagg cccaggcatc ccggcataac cggatcgcca atgaagtggc
    16681 atccgaagaa ccacagatcc ggattgatat ccagttctgc ttcaacatac cctttgtcga
    16741 agttaccacc cgtttcggtc attttgacca cacggtctat catcagcatg ttcggtgctg
    16801 gcaattgcgg gcctttagcg ccaaacagtt caccgtgacc agaggcaaga aggtcttctt
    16861 ttgtatagga ttcgcgttta tctaccatgt tctctgtaag ccttatttta ttgaagcacg
    16921 caggatagct aacacgtgta cgctgaacaa gtccgatcag ttcggaataa accagttcag
    16981 ccaacgtaat ggccatggaa aacggtgacg tccttcctgt tgcgatgctt gcgcgatacg
    17041 ttcctggatg gtttgcatca gcgtcgtttg gccttcgcca tcccacacca gatttaataa
    17101 taacggcagt gcgtcagtca catcgtctac tgcccagatg gtgaatttgc cttcttctac
    17161 cgctttcacc agttcactgt gaagacttaa atggcgaacg ttagcagtgg ggataatgac
    17221 accttgtttc cccgttaact cacgttgctg gcaaatagca aagaagcctt cgattttctc
    17281 atttaaacca ccgaccggct ggacgcggcc gaactgatcg actgaacctg tgatagcgat
    17341 actctgattc actggcacat cagcgagggc gcttatcagg gcgcagagtt cagccatcga
    17401 ggcactatcg ccatccactt cactgtatga ctgctcaaat gtcagcgatg ctgagaaggg
    17461 gatctgttgc tcaagctgta gttccgacat caggaacgcc tgcatgatca tcatcccttt
    17521 cgcatggata ttgccgccaa gctccgcttt gcgttcgatg tcggtgaatt caccatcgcc
    17581 aatatgcaca acgcagctaa tgcgagaagg ttcgccaaaa gcgcgtggat gacccggaaa
    17641 ttcaatgacc gaaagggcgt taatttgccc gatgcgttcg ccttcggttt caatcaggat
    17701 ttgctcctga aggatctcat cctgcatacg ttcagcgagg aaaccttcgc gccattcacg
    17761 ctgctgcagc attaagttta gctgctcgcc ggagaaggtg tcgccatcac acagggaggc
    17821 gacctcttta cactggcgga ggatccactg cgggctaagc ggaagtgttt cttgttcacc
    17881 ggtgtagcgt gctgcttcgc ggataagtac cggccaagca tccgctcccg gggcaggtaa
    17941 gtgattatgt ctggcggtaa atgtcaccca gcgacaccac tggcttactg actccgcatc
    18001 gacaatctgc agagtatctt caaattcgct ataaatagcc tgctctgaaa gctctggctc
    18061 catctcctgg aaatcagcca atgattcgcg ttcgcctacc agaatgactt tcagcttcaa
    18121 tggcattgaa ggcacagaga cggggagagg gcgcgactca tcaaacgcaa cccagtcaaa
    18181 acgctcgcgg ttaacgatat ttttcagccg catccacagc agaggttgcg ccagcagtgt
    18241 acgcaaagag atgatgagaa taccgccatt tgcctgatgc accagaccag gctgcagggt
    18301 aatgtcgcca ttaaactggc gcaggcagcc aaagagttgc tccgcttcta cccagtcggc
    18361 agcgacaact tgcgttaaag tcgcaaaatt atcatctgca ctcactgcgt gacgtaagcg
    18421 gatggagtgg ccggaaacct catagtgatc gcccaccagt tggcctgcat cgctttgtag
    18481 cgtacgcgcg gcatcggcaa taagattcag atactcagac tcttccgggg ccttagccag
    18541 cataaaagag gaggatgctc gcgtatgcag caactgctcc agcgcaaatt gcagtcgcgg
    18601 ttgagtatca ctgaataaag gatcgttttc gtcaatcaaa tgtggctgag caaatatttc
    18661 ctgatagcta tcggtatcag gaaccaggtc acgccatgca agtttcgtaa tggtcaaagt
    18721 tgatgttttt tagtctgttg tcaaagccgc gattataccg taaccggcac tacagcacac
    18781 gtagaaagca ccgacaatac tcctggctgg gcgttaaagc tcacaggatg gagattcttt
    18841 tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca ttgagatcgc
    18901 tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt atctggtgaa
    18961 gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg cccaggaagc
    19021 cgtcgatgtg ttgctctcgc tcgaaaatga acccgtgctg gtaaatggct ggattgacaa
    19081 gcatatgaat ccggaactgg tcaatcgcat gaagcagacc attcgggcca gacggaagcg
    19141 ccattttaat gcggaacacc agcatacgcg caaaaaatcg atcgatctgg aatttattgt
    19201 ctggcaacgt ctggctggtc ttgcacagcg tcgcggtaaa acgctttctg aaacgattgt
    19261 tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt cttctcttaa
    19321 gcaggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt caatttacta
    19381 aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact taagcctgcg
    19441 gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga tccggagcca
    19501 agcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt
    19561 tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg gagatcaggt
    19621 aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg tcagaaccga
    19681 tgcggtcagt gtaacccaga acaactacgg aaccgtcttt cggatctagg ttgctcagct
    19741 ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct ttgttgaagt
    19801 tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt gccggagctg
    19861 gagcaactac cggagctgct tcgccctgac cgaaacggta ggaaacaccc aggctcagca
    19921 gaccgttgtc cggacgagta ccgatggtgt ttgcgtcacc gatgttgttg gtccactggt
    19981 attccagacg ggtagcgatt tcaggagtga tcgcgtactc aacaccacct gcgaaaaccg
    20041 gagaaacgcc agtgtcgtgg tctttaaagg atgcgccacc aggtacgtta gccttggtgt
    20101 ctgcacgcca taccatacca cccagacgag tgtagatgtc cagatcgtca gtgattgggt
    20161 aacccagttt agcggtcagc tgaacgccct gagctttgta tgcgccgttg atgttgtcgc
    20221 ctttgtacgg catacgacct aaccagtcgt aacccatttc aaaaccaaca tacgggttaa
    20281 cctggtaacc accaaaagca cctgcaccca gttggttttc gtgggtcgga ccattgttag
    20341 gaataaaacc agtgtcatgg tactgggacc agcccagttt agcaccagtg taccaggtgt
    20401 tatctttcgg agcggcctgc gctacggtag cgaaaccagc cagtgccact gcaatcgcga
    20461 tagctgtctt tttcattttt tgcgcctcgt tatcatccaa aatacgccat gaatatctcc
    20521 aacgagataa cacggttaaa tccttcaccg ggggatctgc tcaatattaa ctctaccgat
    20581 atcttcggct tatgccgagc acccctggcg atgtaaagtc tacaacgtag ttgaaaactt
    20641 acaagtgtga actccgtcag gcatatgaaa aaaaagtctt gtataaggta tgtttaatct
    20701 ttttgtcagc gacaatttac agaaaaaaaa cgcggaaacc gcttcagaca agccttcgca
    20761 aggaaaatta gtcacgactg aaagcattgg ctgggcgaca aaaaaagttc taggattaat
    20821 cctaaattta cttaatgata caaattagag tgaattttta gcccggaaag ttgtctcgtt
    20881 gtgtgagagg atgcgcttac cggacgcata ataaacccca tagcgttacc ttcatttgcc
    20941 gcatcaacaa gttcagcatg ctcttcttca gtcaaatcat ctgtcaacca accgatcacc
    21001 acactgtaat tgcccgtgcg taaagcgcga accattgact ccactgtgtg gcaaggggag
    21061 agctggctaa tctgcattac tttcgttaag ggtagcccag atgcctgaac ccattcccga
    21121 ctcagttttt gttgcggtgt taaccagagt tgccagcgcg attgctgacc gagttgctgt
    21181 aacaatggca acagtagaag ttgcgtcatc atgggctgat cttcgcgata gacaacttca
    21241 ctgataagcc cggctgtagt gttttccgta gagacacgcg caattttact tgctgcggat
    21301 gagaacgacg aagaacgatg tgcatagcct gaagtgtaca taatcaatcc agcccctgtg
    21361 agttactgta tggatgtgca gtacatccag tgacaacaaa gatcaaccct attttcggaa
    21421 agagcctcgc aaattttgtc gttggtgacg ggaaaacata aattaatctt gccccttaag
    21481 aataagttgc ctattttcgt agttaacgga tccgttaatg tgaatcattc ttttatgtta
    21541 tgattttaaa aggaatttta tgaaaagcct ctcctataag cggatctata aatcacaaga
    21601 atacctggca acgttgggca caattgaata ccgatcattg tttggcagtt acagcctgac
    21661 cgttgacgac acggtgtttg cgatggtttc tgatggtgag ttgtatcttc gggc
//
LOCUS       DAJTYM010000067        20557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTYM010000067 DAJTYM010000000
VERSION     DAJTYM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..446
                     /locus_tag="OO575_002588"
     CDS             <1..446
                     /locus_tag="OO575_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6665505.1"
                     /translation="RKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPH
                     EHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLD
                     EFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            548..700
                     /gene="hokA"
                     /locus_tag="OO575_002589"
     CDS             548..700
                     /gene="hokA"
                     /locus_tag="OO575_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6665506.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(887..1099)
                     /gene="cspA"
                     /locus_tag="OO575_002590"
     CDS             complement(887..1099)
                     /gene="cspA"
                     /locus_tag="OO575_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6665507.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1380..1670)
                     /locus_tag="OO575_002591"
                     /pseudo
     CDS             complement(1380..1670)
                     /locus_tag="OO575_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2104..2814
                     /locus_tag="OO575_002592"
     CDS             2104..2814
                     /locus_tag="OO575_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6665508.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2864..3838)
                     /gene="ghrB"
                     /locus_tag="OO575_002593"
     CDS             complement(2864..3838)
                     /gene="ghrB"
                     /locus_tag="OO575_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6665509.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3942..4592)
                     /locus_tag="OO575_002594"
     CDS             complement(3942..4592)
                     /locus_tag="OO575_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6665510.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4755..6953
                     /locus_tag="OO575_002595"
     CDS             4755..6953
                     /locus_tag="OO575_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6665511.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6922..7362)
                     /locus_tag="OO575_002596"
     CDS             complement(6922..7362)
                     /locus_tag="OO575_002596"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6665512.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7359..7922)
                     /gene="tag"
                     /locus_tag="OO575_002597"
     CDS             complement(7359..7922)
                     /gene="tag"
                     /locus_tag="OO575_002597"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6665513.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8080..8778
                     /locus_tag="OO575_002598"
     CDS             8080..8778
                     /locus_tag="OO575_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6665514.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9007..10215
                     /locus_tag="OO575_002599"
     CDS             9007..10215
                     /locus_tag="OO575_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6665515.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10539..12230
                     /gene="eptB"
                     /locus_tag="OO575_002600"
     CDS             10539..12230
                     /gene="eptB"
                     /locus_tag="OO575_002600"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6665516.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12322..12398
                     /locus_tag="OO575_002601"
     tRNA            12322..12398
                     /locus_tag="OO575_002601"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12356..12358,aa:Pro,seq:cgg)
     gene            13142..14749
                     /gene="dppA"
                     /locus_tag="OO575_002602"
     CDS             13142..14749
                     /gene="dppA"
                     /locus_tag="OO575_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6665517.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15057..16076
                     /gene="dppB"
                     /locus_tag="OO575_002603"
     CDS             15057..16076
                     /gene="dppB"
                     /locus_tag="OO575_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6665518.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16086..16988
                     /gene="dppC"
                     /locus_tag="OO575_002604"
     CDS             16086..16988
                     /gene="dppC"
                     /locus_tag="OO575_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6665519.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16999..17982
                     /gene="dppD"
                     /locus_tag="OO575_002605"
     CDS             16999..17982
                     /gene="dppD"
                     /locus_tag="OO575_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6665520.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17979..18983
                     /gene="dppF"
                     /locus_tag="OO575_002606"
     CDS             17979..18983
                     /gene="dppF"
                     /locus_tag="OO575_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6665521.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19013..20284)
                     /locus_tag="OO575_002607"
     CDS             complement(19013..20284)
                     /locus_tag="OO575_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6665522.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4963 a         5057 c         5377 g         5160 t
ORIGIN      
        1 ggcgcaagct gtatttgtct ccagtaatag atctcttcaa caacgaagtt atttcttaca
       61 gcctttcgga aagaccagtg atgaacatgg ttgagaatat gctcgatcag gcattcaaaa
      121 agcttaatcc tcacgagcat cctgttctgc actctgacca gggatggcag tatcgtatga
      181 gaagatatca aaatatcctt aaagaacatg gtattaaaca aagcatgtcc agaaaaggca
      241 attgtctgga taatgctgtg gtggagtgtt tctttggaac cttaaagtcg gagtgttttt
      301 atcttgatga gttcagtaat ataagcgaac tgaaggatgc tgttacggaa tatattgaat
      361 actataacag cagaagaatt agcctgaaat taaaaggtct gactccaatt gaatatcgga
      421 atcagaccta tatgcctcgt gtataactgt ccaacttttt ggggtcagta caaagtgagg
      481 ctaccctatg cctgaacacc ataaggttag cctcttactc gttggaaatc aacacagggg
      541 gctgggaatg ccgcagaaat atagattact ttctttaata gtgatttgtt tcacgctttt
      601 atttttcacc tggatgataa gagattcact gtgtgaattg catattaaac aggagagtta
      661 tgagctggcg gcgtttttag cctacaaatt gaaagagtaa gagtcttcga cgggaaatta
      721 ttcccgcctt acttacggcg ttgcgcattc tcattgcacc catatttatt cttcacaaaa
      781 ataataatag attttattac gcgatcgatt atttatttcc tgaaaaaaaa taaaaaaatc
      841 cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac aggctggtta
      901 cgttaccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact ttctgacctt
      961 cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac acatctttag
     1021 agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat tttacgatac
     1081 cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct ttcggcgata
     1141 tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg gtatcaacga
     1201 taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt caaaccgttg
     1261 atgtgcatta agccactcat tggcgggtga tgcaacaatt atttttcata tttatgatta
     1321 atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg gataaaagtc
     1381 tatttcatca actgcttact taatgccggg ttggcttgaa tcaaacgcat caattttagt
     1441 tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc gactgatacg
     1501 cccaaaaccc gggcgaaatc atcgattttt aatcctgtcc cttttcataa ctgctcaatt
     1561 tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc tttaaaaaca
     1621 atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat tgaaaactcc
     1681 tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag tcgggaaaag
     1741 cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca gcctgtcatt
     1801 tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct tatcactcct
     1861 gttttgtaaa gatttcgcaa atctaacctt ctgcgaaggc gtaaatctct ggctacaaaa
     1921 aaggcaacgg cactatcatt ttttaacaat gaaacaacaa attaaccttg tttcattttc
     1981 tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg tcctggacta
     2041 tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga aaggagtaag
     2101 aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc gttattaatg
     2161 gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc gtttatcgac
     2221 ttcctgcaaa atacagtgat gcgtagcggt gaacgtctac caaccctgac tgccgatcag
     2281 aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta ttctcagcag
     2341 gtgaatcagg cgatggattc cggtctgcgc ccggtcgtag acagcgttaa cgcaattcgc
     2401 gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg ttcgctgggt
     2461 gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca ctctgcgcta
     2521 aaacagagcg atgacctgaa accggtattt gatcaggcat tcaccaaagt ggtaacgacg
     2581 ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca acaactggtg
     2641 atggttggag actacattgc tcagcaaggt actcaggtga gctttgtcgc aaatggcatc
     2701 cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc attaccggca
     2761 caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca ataaagagta
     2821 aaagcccgag cgattatcgg gctttacttc acgcagtcgc agcttagtcc gcgacgtgag
     2881 gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta tccacggcac
     2941 aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt actgcgacga
     3001 cgttggccat tgagagcaac ggcgaatcta cggacagtgg ctcttgttcg aagacatcca
     3061 gcccggcggc gtgaatttcg cctttctgca atgctgcgat cagtgcattt tcgtcaacca
     3121 ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg aattgttctg
     3181 cgccaaacag atgatgcgtc tcatcagtta acggcaggat caggcaaacg aaatctgact
     3241 cttgtaacag agtatccaaa tcgcagtagc gggcgttgaa gcgttcttct gcttctttat
     3301 ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcaca cgttgtgcca
     3361 gcgccatgcc gatccgtccc atcccgacaa tgcccagtgt tttatggtga acgtcagtgc
     3421 cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt tctgctacct
     3481 ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg acggtttctg
     3541 ttaatacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca tcgacatcaa
     3601 aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt ttttccagca
     3661 atgcggcatc tacattctcg tttgaaccca gtaaaccttc agcttctgca aaaattgctg
     3721 cattttgaac gacggtttgt gggctgaggt ttgccacctg gtgaacggtg aaatgcgctt
     3781 gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac ggcttcatgc
     3841 ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa ggaaaactga
     3901 agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg gcttaaggta
     3961 atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc gattgggtta
     4021 gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt gatcaacgcg
     4081 ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcaggtcgtg accccccgtg
     4141 ctgtcggtat aaccaatcac gttaaccgcc gttttcggat actctttcag caccattgcc
     4201 acgccggtca gggtgttagc gcctgccggt ttcaggggag cgctgctgct gtcgaaggtc
     4261 acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct aacaccagtg
     4321 ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc cacgccgccg
     4381 cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc tttcttcgaa
     4441 gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc gatagcagat
     4501 ttgcctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac cgccagagcg
     4561 ccactcacta cggcggcaag taagataaac acgtttcttc attgttaatc cttaataacc
     4621 tttttattct ttgtcacggg ttccgtggcg ggagattatg ccgcgtgaac atgaagatta
     4681 ttcctgggaa tactcggaaa tttgtaagta atatttaact gctcaataca tctaaccttt
     4741 caggagtctt cggtttggcc aactcatcct cacgatattc cgttctgact gccgcccact
     4801 gggggcccat gctggttgaa accgacggcg aaaccgtgtt tagctcgcgt ggcgcgttag
     4861 ccacaggaat ggaaaactcc ttgcagagcg cggttcgcga ccaggttcac agcaatacgc
     4921 gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc accggaaaac ccgcaaggca
     4981 ttcgtgggca ggatgaattt gttcgcgtga gttgggatga ggcgctggca ggcggttata
     5041 ccgggcatct gggggattat tcgaccggtg cggcacaggc gatcatgccg tatgtcgtag
     5101 gtggcagcga agtttatcaa cagcaaacca gttggccgct ggtgctggaa catagcgatg
     5161 tcgtggtgtt gtggagtgct aacccactca atacgctgaa aattgcgtgg aatgcatccg
     5221 atgagcaggg gctttcttac ttttccgcac tgcgtgacag tgggaaaaag ctgatctgca
     5281 ttgatccaat gcgatcggaa accgtcgatt tctttggcga taaaatggag tgggtggcac
     5341 cgcacatggg caccgatgtt gcgctgatgc tggggatcgc ccatacgctg gtggaaaatg
     5401 gttggcacga cgaagcgttt ctggcgcgtt gcaccacagg ttatgccgtc ttcgcctctt
     5461 atttgctggg cgagagtgac ggaatagcga aaaacgccga atgggcggca gagatttgtg
     5521 gtgttggcgc agcgaaaatc cgcgagctgg cggctatttt ccaccaaaat accaccatgc
     5581 tgatggcagg ctggggaatg cagcgccaac agtttggtga gcaaaaacac tggatgatcg
     5641 tcacgctggc agcaatgttg gggcaaatcg gcacacccgg cggcggtttt ggtctttctt
     5701 accattttgc caatggtggt gaccccacgc ggcgttctgc ggtgctctct tccatgcagg
     5761 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     5821 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     5881 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     5941 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     6001 cggcgaaaca cgcggatatt gttctgcctg cgactacctc ttttgagcgt aatgatctca
     6061 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     6121 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     6181 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     6241 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     6301 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     6361 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     6421 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     6481 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     6541 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     6601 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     6661 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     6721 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     6781 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     6841 ccagctcgcg gctggggaat ggctgtgcgg gtaatacgac gctggcatgg ctggaaaaat
     6901 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     6961 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     7021 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     7081 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     7141 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     7201 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     7261 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     7321 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccagatag
     7381 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     7441 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ccttagatag ggcgtcggag
     7501 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     7561 tttacaaacg accagacaaa gtcgggaaac ggttcgccgt tctgttccat ttgcaggtac
     7621 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     7681 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     7741 tgaaagtagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     7801 tgctgccctt caaagcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     7861 cactcattat catggtaggc aatataaagc gggccctgac tcacccagcc gcaacgttcc
     7921 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     7981 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     8041 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     8101 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     8161 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     8221 tacacctggg atagtgatca tcaacctgat tacaacgata ttttgtcgca acgtattcaa
     8281 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     8341 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     8401 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     8461 actaccacgc tgaccgatcc gttatggcat gccagcgtga gtagcttagg ctggcgtgtt
     8521 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     8581 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     8641 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     8701 gctgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     8761 gttagcgcca gattttaacg taacagtcac aattgaaacc attgaataac aacagttgtg
     8821 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     8881 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaagc
     8941 ataaaatgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     9001 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     9061 attacccagt ttgcgctggg gtcggtttat acccggagcc tgtttaatgg cgcgctttct
     9121 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     9181 ctggcaattt cgtcttctgt tgcgggcaaa ttgcaggaac gttttggcgt taaacgcgtc
     9241 accgtggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     9301 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     9361 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     9421 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     9481 ctcgaaacgg tgggtctgga aaaaaccttt gtgatttggg gagcgattgc gctggtgatg
     9541 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     9601 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     9661 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     9721 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     9781 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     9841 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     9901 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     9961 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
    10021 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
    10081 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
    10141 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
    10201 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
    10261 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
    10321 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
    10381 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
    10441 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgctaa
    10501 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
    10561 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
    10621 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
    10681 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
    10741 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
    10801 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
    10861 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
    10921 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
    10981 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggtggtcgtc gtactggcgg
    11041 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
    11101 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
    11161 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
    11221 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgata
    11281 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
    11341 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
    11401 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
    11461 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
    11521 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
    11581 tggcagacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
    11641 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
    11701 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
    11761 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
    11821 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
    11881 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
    11941 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
    12001 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
    12061 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
    12121 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
    12181 acaacaactg gtgtcacatc ccgcagacaa aagtggcagc ggctaactaa gcggcctgct
    12241 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
    12301 cagtaagatg cgccccgcat tcggtgattg gcgcagcctg gtagcgcact tcgttcggga
    12361 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcaacg
    12421 agcaggtttt tttgcatttg tagctcatga ggatgagaac ctccgggggc agggaggctc
    12481 gactcgacgt aggcctgata agacgcgcca gcgtcgcatc aggcaacggc accgcactta
    12541 ttgtcggatg cggcgcgacc gccttattcg accaacacac cacacatttt cctgccaact
    12601 ttgcgcccca tatctcacta tccattaacg gatttgtgac agaaaccatt gtcttttttt
    12661 atatttgctt aaatcttttt tgcgttactt atggctggcg ctggcatttt ccgctgtgcg
    12721 ttttagcgtt catggtatta atcgctcaga tatccacctt ttcctcagat ttttttcatt
    12781 aaatgcataa aacttaatca ccaaatgttg ctaaatatta agctgattgt tctggttatg
    12841 ttggagtagc acaaatttct ctgctgcaaa tagctgtttg aagttttttt aatctttgtt
    12901 tgtggattct caccgttggt gtaaatcccg gttggctgta ttgacgtttt cacattctgt
    12961 tgacagattg taggtcacga ggggcatttt atggaggatc cgcactgtta cactgatgtt
    13021 aattagtacg gcatccccac ctcataacgt tgacccgacc gggcaaaaaa caaaaaaggt
    13081 caggcagcga caacccactg caaagggtta aaacaacaaa catcacaatt ggagcagaat
    13141 aatgcgtatt tccttgaaaa agtcagggat gctgaagctt ggtctcagcc tggtggctat
    13201 gaccgtcgca gcaagtgttc aggctaaaac tctggtttat tgctcagaag gatctccgga
    13261 agggtttaac ccgcagctgt ttacctccgg caccacctat gacgcctctt ccgtcccgct
    13321 ttataaccgc ctggttgaat ttaaaatcgg caccaccgaa gtgatcccgg gcctcgctga
    13381 aaagtgggaa gtcagcgaag acggtaaaac ctataccttc catctgcgta aaggtgtgaa
    13441 gtggcacgac aataaagaat tcaaaccgac gcgcgaaatg aacgccgatg acgtggtgtt
    13501 ctcgttcgat cgtcagaaaa acgcgcaaaa cccgtaccat aaagtttctg gcggcagcta
    13561 cgaatacttc gaaggtatgg gcttgccgga gctgatcagc gaagtgaaaa aggtggacga
    13621 taacaccgtt cagtttgtgc tgactcgacc ggaagcgccg ttcctcgctg acctggcaat
    13681 ggacttcgcc tctattctgt caaaagaata tgctgacgcg atgatgaaag ccggtacacc
    13741 ggaaaaactg gacctcaacc caatcggaac cggtccgttc cagttacagc agtaccaaaa
    13801 agattcccgt atccgctaca aagcgtttga tggctactgg ggcaccaaac cgcagatcga
    13861 tacgctggtc ttctctatta cccctgatgc ttccgtgcgt tacgcgaaat tgcagaagaa
    13921 tgaatgccag gtgatgccgt acccgaaccc ggcagacatc gctcgcatga agcaggataa
    13981 atccatcaac ctgatggaaa tgccggggct gaacgtcggc tacctctcgt ataacgtgca
    14041 gaaaaaaccg ctggatgacg tgaaagttcg ccaagcacta acctacgcgg tgaacaaaga
    14101 cgcgatcatc aaagcggttt atcagggcgc gggtgtatca gcgaaaaacc tgatcccgcc
    14161 aaccatgtgg ggctataacg acgacgttca ggattacacc tacgatcctg aaaaagcgaa
    14221 agccttgctg aaagaagcgg gtctggaaaa aggtttctcc atcgacctgt gggcgatgcc
    14281 ggtacaacgt ccgtataacc cgaacgctcg ccgcatggcg gagatgattc aggcagactg
    14341 ggcgaaagtg ggcgtgcagg ccaaaatcgt cacctacgaa tggggtgagt acctcaagcg
    14401 tgcgaaagat ggcgaacatc agacggtaat gatgggctgg actggcgata acggggaccc
    14461 ggataacttc ttcgccaccc tgttcagctg cgccgcctct gaacaaggtt ctaactactc
    14521 aaaatggtgc tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta ccgacgacca
    14581 taacaaacgc gttgaactgt acaagcaagc gcaggtggtg atgcacgatc aggctccggc
    14641 actgatcatc gctcactcca ccgtgtttga accggtacgc aaagaagtca aaggctatgt
    14701 ggttgatcca ttaggcaaac atcacttcga aaacgtctct atcgaataat taaaagccat
    14761 acaagactga tggcaaaggc aaaactgcct gatgtgctcc gcttatcagg cctacgaaaa
    14821 ttctgcaatg tattgaattt gcacgatttt gtaggccgga taaggcgtta acgccgcatc
    14881 cggcataaac aaagcgcact ttgtcaacaa tctgtatacc cggtggcgct gtgcctctgc
    14941 gtgcagcgcc atccggcagc aatacttcat tctctgtccg acaagggcgg gaattgattt
    15001 gtgagcaata cagacacgca gttccaggct gcgagtcact atagagaatc cgggttatgt
    15061 tgcagtttat tctccgacgt ttgggactcg tcatccccac gtttatcggt attacccttc
    15121 tcacatttgc ctttgtccac atgatcccgg gcgatccggt gatgatcatg gcaggcgaac
    15181 gtgggatctc ccctgagcgt cacgtgcagc tgctggctga actcggctta gataaaccga
    15241 tgtggcagca gtatctccat tacatttggg gcgtaatgca cggcgatcta ggcatttcaa
    15301 tgaaaagccg aattccagta tgggaagagt tcgtgccgcg ctttcaggcc acgctggaac
    15361 ttggcgtctg cgcgatgatt tttgctacgg cagtcggtat tccggttggt gtgctggctg
    15421 cggttaaacg cggttccatt ttcgatcaca ctgcagttgg cctggcgctg accggctact
    15481 cgatgccgat cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg cactggaacc
    15541 tgacgcccgt ctccggtcgc gtgagcgata tggtgttcct cgatgactcc aatccgttaa
    15601 ccggttttat gctaatcgac accgccatct ggggtgaaga cggcaacttt atcgatgccg
    15661 tcgcccatat gatcttgccc gccattgtgc tgggtactat tccgctagcg gtcattgtgc
    15721 gtatgacacg ctcctcgatg ctggaagtgc tgggcgagga ttacatccgc accgcgcgcg
    15781 ccaaagggct aacccgcatg cgggtgatta tcgtccatgc gctgcgtaac gcgatgctgc
    15841 cggtagtgac cgttatcggc ctccaggtgg gaacattgct ggcgggggcg attctgaccg
    15901 aaaccatctt ctcgtggccc ggtctggggc gctggttgat tgacgcactg caacgccgcg
    15961 actatccggt agtgcagggc ggcgtattgc tggtggcgac gatgattatc ctcgtcaact
    16021 tgctggtcga tctgctgtac ggcgtggtga acccgcgtat tcgtcataag aagtaagggg
    16081 acatcatgtc acaggttact gaaaataaag tgattagcgc accggtgccg atgaccccgt
    16141 tacaggagtt ctggcactat tttaaacgca acaaaggcgc ggtcgtcggg ctggtttacg
    16201 tcgccatcgt gctgttcatc gcgatctttg ccaactggat tgcgccttat aacccggcgg
    16261 atcagttccg cgatgcgctg ctcgccccgc cagcctggca ggaaggcggc agtatggcac
    16321 acttgctggg caccgatgac gtcggtcgtg atgtgctgtc gcggctgatg tacggcgcgc
    16381 gtttatcgct gctggttggc tgtctggtgg tggtgttgtc gctgattatg ggcgttattc
    16441 tcggcctgat cgccggttac ttcggcggcc tggttgataa cattatcatg cgcgtggtcg
    16501 atatcatgct ggcgctgcca agtctgctgt tggcactggt gctggtggcg attttcggcc
    16561 cgtcgattgg taacgccgcg ctggcgctga ccttcgttgc cttgccgcac tatgtgcgct
    16621 taacccgcgc cgccgtgctg gtggaggtga atcgcgatta cgtcaccgcc tctcgcgtgg
    16681 caggtgccgg ggcgatgcgc cagatgttta ttaacatctt cccgaactgc cttgcgccgc
    16741 tgattgttca ggcgtcgctc ggtttctcta acgccattct cgatatggct gctcttggtt
    16801 tcctcggcat gggggcacag ccgccaacgc ctgagtgggg caccatgctc tccgacgtgt
    16861 tgcagttcgc gcaaagcgcc tggtgggtcg tgaccttccc tggtctggcg atcctgttga
    16921 cggtgctggc atttaacctg atgggtgacg gtctgcgtga cgcgctcgat cccaaactaa
    16981 agcagtaaga ggttcgagat ggcgttatta aatgtagata aattatcggt gcatttcggc
    17041 gacgaaagcg caccgttccg cgccgtagac cgcatcagct acagcgtaaa acagggcgaa
    17101 gtggtcggga ttgtgggtga gtccggctcc ggtaagtcgg tcagttcact ggcgattatg
    17161 gggctgattg attatccggg ccgcgtaatg gcagaaaaac tggagtttaa cggccaggat
    17221 ttgcagcgta tctcagaaaa agagcgccgc aacctggtgg gtgccgaagt ggcgatgatc
    17281 ttccaggacc cgatgaccag ccttaacccg tgctacaccg tgggtttcca gattatggaa
    17341 gcgattaaag tgcatcaggg cggcaacaaa agtacccgcc gtcagcgggc gattgacctg
    17401 ctgaatcagg tcggtattcc cgatccggct tcgcgtctgg atgtttaccc gcatcagctt
    17461 tccggcggca tgagccagcg cgtgatgatc gccatggcga ttgcctgtcg gccaaaactg
    17521 ctgattgccg atgaaccgac caccgcgctg gacgtgacca ttcaggcgca aatcatcgaa
    17581 ctactgctgg agctacagca gaaagagaac atggcgctgg tgttaattac ccatgacctg
    17641 gcgctggtgg cggaagctgc gcataaaatc attgtgatgt atgcaggtca ggtagtagaa
    17701 acgggcgatg cacacgccat cttccatgcg ccgcgtcacc cgtatactca ggcattgctg
    17761 cgtgcgctgc cagaatttgc tcaggacaaa gaacgtctgg cgtcgttgcc aggtgttgtt
    17821 cccggcaagt atgaccgccc gaacggctgc ctgcttaacc cgcgctgccc ctatgccact
    17881 gacagatgtc gcgctgaaga accggcgctg aatatgctcg ctgatgggcg tcagtccaaa
    17941 tgccattacc cacttgatga tgccgggagg cccacactat gagtacgcaa gaggccacct
    18001 cgcaacaacc gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg aagaaaggca
    18061 tgttcgtgcc ggaacgtctg gtgaaggcgc tggacggcgt ttcgtttaac cttgaacgtg
    18121 gcaaaacgct ggcagtagtg ggcgaatctg gctgcggtaa atcgaccctc ggtcggttgc
    18181 tgacgatgat cgaaacgccc accggcggcg agctgtatta tcaggggcag gatctgctca
    18241 agcacgatcc gcaggcgcag aagctgcgtc ggcagaaaat ccagatcgtc ttccagaatc
    18301 cctacggttc gctgaatccg cgtaagaaag ttgggcaaat tctcgaagag ccgctgctta
    18361 ttaacaccag cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg atggcaaaag
    18421 tcggcctgaa aaccgagcac tatgaccgct atccgcacat gttttccggc ggacaacgtc
    18481 agcgtatcgc tattgcccgt ggtctgatgc tcgacccgga tgtggtgatt gccgatgagc
    18541 cggtttctgc gctggacgtc tcagtgcgtg cgcaggtgct gaatctgatg atggatttgc
    18601 agcaggagtt ggggctttct tatgtcttta tctcccacga cctgtcggtt gtggagcaca
    18661 ttgctgatga agtgatggtg atgtacctag gccgctgcgt ggagaaagga acgaaggacc
    18721 aaatcttcaa taacccgcgc catccgtaca cccaggcgct gctctccgcg acgccacgcc
    18781 tgaacccgga cgatcgtcgt gaacgcatca aactcaccgg tgaactgcca agcccgctga
    18841 atcctccgcc gggttgcgcc ttcaacgccc gctgtcgtcg gcgcttcggc ccctgcaccc
    18901 agttgcagcc gcagctaaaa gactacggag gtcaactggt agcttgtttt gctgttgatc
    18961 aggatgaaaa tccgcagcgt taactcacaa aaccggggcc atgcgctccg gtttactcaa
    19021 tcagcttcaa cagtggcgac aagcatagca aaatgccgta aagcagaatc agccaggctt
    19081 taaatccgcg aagtttttcc agttgtgcga ctttatagat caggaaaaac ggaatgaggc
    19141 acgacacaat gccatataac gggcttccca actgaaagaa caccagcact gatacacgaa
    19201 acgaaaccca aatcgtcaac gtaatgacga taaaagcgca gatcgccaga gtcagcatgc
    19261 gtgagttaat tttcttggta tcaataattc ggcttaacag attgagaata atgcctttaa
    19321 tagcctcgtg gaaaccgaga taaatgccaa agaatgcggt cagtacggca aagatattca
    19381 gcaccgtaga ggtgatatga atgatatgtc cagggatcac ctgcgcggcc agtgccagtg
    19441 ccgagatatt ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag aaggtaaacg
    19501 aaaaggcaaa aaacaggatc accgcgatga gcgtgatata actaattcgg tgggtacgca
    19561 gcgcgagccg ggttgccagt actttatctg cttcccgttt acgataggca atattcattg
    19621 ggttaagtac ctgaataaat accgcagaaa agaagcaaaa tggaatggta agcaggacat
    19681 cgcggaaaaa gacggaggct tgcgggaagg cggttatatt ggcgaaattc cagtgcggga
    19741 tcatcgcaaa accgaacacg acaataatcc ctactttgac caccaccatt ggcccagaaa
    19801 tcttaaacag taatcgttca ccaccagacg caatcgctat cagtacggcg aaaatagcga
    19861 ctttataaag tagagattgt gaaagatcgg catcggttaa accgaaggtt ttcaggtacg
    19921 aggcgctgtc gaaaaccacg gagagagagt agataaaaat cccgtggata atcatcaaaa
    19981 agtagataac cccgaggaaa attccccagt tcttccccag gtaatgactg ataatatcgg
    20041 tgtagtcatt acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc tgcactaccc
    20101 aggtgacagg ataagcaatg atcgctgcgg taataaatac ccaaattccc ttcaagccaa
    20161 tttgtactgg catcagcacg gttccggcac caatcgccat gcctatgcat aataaaaccc
    20221 agccgaagtc gtagcgtgta aacggcaatt tctgattgtg tttcgatagt gtgttgtgct
    20281 gcataaagtc ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc cagtttctct
    20341 tgcgcaacag gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa cagattcact
    20401 ggggaggtcg cgaggaggcg caattctaca aaattgcgca tttcactacc agtctggagt
    20461 cagcccggat atgaactgca actattgcaa ataaagaaat taagctggaa ggcatcgcgc
    20521 aatgaggaag attgccgggt gctgacagta aaaagca
//
LOCUS       DAJTYM010000068        20538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTYM010000068 DAJTYM010000000
VERSION     DAJTYM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..856)
                     /gene="fbp"
                     /locus_tag="OO575_002608"
     CDS             complement(<1..856)
                     /gene="fbp"
                     /locus_tag="OO575_002608"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6665523.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQ"
     gene            1032..2405
                     /gene="mpl"
                     /locus_tag="OO575_002609"
     CDS             1032..2405
                     /gene="mpl"
                     /locus_tag="OO575_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6665524.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2488..3039)
                     /locus_tag="OO575_002610"
     CDS             complement(2488..3039)
                     /locus_tag="OO575_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6665525.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3133..4485
                     /gene="pmbA"
                     /locus_tag="OO575_002611"
     CDS             3133..4485
                     /gene="pmbA"
                     /locus_tag="OO575_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6665526.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4668..5050
                     /gene="cybC"
                     /locus_tag="OO575_002612"
                     /pseudo
     CDS             4668..5050
                     /gene="cybC"
                     /locus_tag="OO575_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5095..5559)
                     /gene="nrdG"
                     /locus_tag="OO575_002613"
     CDS             complement(5095..5559)
                     /gene="nrdG"
                     /locus_tag="OO575_002613"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6665527.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5717..7855)
                     /gene="nrdD"
                     /locus_tag="OO575_002614"
     CDS             complement(5717..7855)
                     /gene="nrdD"
                     /locus_tag="OO575_002614"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6665528.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8249..9904)
                     /gene="treC"
                     /locus_tag="OO575_002615"
     CDS             complement(8249..9904)
                     /gene="treC"
                     /locus_tag="OO575_002615"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6665529.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(9954..11375)
                     /gene="treB"
                     /locus_tag="OO575_002616"
     CDS             complement(9954..11375)
                     /gene="treB"
                     /locus_tag="OO575_002616"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6665530.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11494..12441)
                     /gene="treR"
                     /locus_tag="OO575_002617"
     CDS             complement(11494..12441)
                     /gene="treR"
                     /locus_tag="OO575_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6665531.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12626..12679
                     /gene="mgtL"
                     /locus_tag="OO575_002618"
     CDS             12626..12679
                     /gene="mgtL"
                     /locus_tag="OO575_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6665532.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12820..15516
                     /gene="mgtA"
                     /locus_tag="OO575_002619"
     CDS             12820..15516
                     /gene="mgtA"
                     /locus_tag="OO575_002619"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6665533.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15722..16108)
                     /gene="ridA"
                     /locus_tag="OO575_002620"
     CDS             complement(15722..16108)
                     /gene="ridA"
                     /locus_tag="OO575_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6665534.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16181..16642)
                     /gene="pyrI"
                     /locus_tag="OO575_002621"
     CDS             complement(16181..16642)
                     /gene="pyrI"
                     /locus_tag="OO575_002621"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6665535.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16655..17590)
                     /gene="pyrB"
                     /locus_tag="OO575_002622"
     CDS             complement(16655..17590)
                     /gene="pyrB"
                     /locus_tag="OO575_002622"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6665536.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17594..17728)
                     /gene="pyrL"
                     /locus_tag="OO575_002623"
     CDS             complement(17594..17728)
                     /gene="pyrL"
                     /locus_tag="OO575_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6665537.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18009..18404)
                     /locus_tag="OO575_002624"
     CDS             complement(18009..18404)
                     /locus_tag="OO575_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6665538.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18536..19249)
                     /gene="bdcA"
                     /locus_tag="OO575_002625"
     CDS             complement(18536..19249)
                     /gene="bdcA"
                     /locus_tag="OO575_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6665539.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19320..19913
                     /locus_tag="OO575_002626"
     CDS             19320..19913
                     /locus_tag="OO575_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6665540.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20058..20510
                     /gene="tabA"
                     /locus_tag="OO575_002627"
     CDS             20058..20510
                     /gene="tabA"
                     /locus_tag="OO575_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6665541.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4881 a         5166 c         5449 g         5042 t
ORIGIN      
        1 cttgttccgc caggaatgcc atcgggttgc actcataaag caaacgcagt ttgccgtccg
       61 ggtggctggc ggtgcttggg tagagataaa taccgccttt cagcaggtta cggtggaaat
      121 ccgcaaccag tgaaccgata taacgcgagg tatacgggcg attggtggat ttatcttctt
      181 cctggcagaa tttaatgtac ttcttcaccc cgttcggaaa cttaatgtag tttccttcgt
      241 tgatggagta ggttttgcct ttctccggga agcgcatccg ttcctggcac aggcagaaaa
      301 cgcccagcga aggatcgtaa gtaaaggcgt gaacaccgca tccggtggtg taaaccagca
      361 tggtagagga gccgtatacc acgtaacctg ccgcaacctg tttgttacca ggctggagga
      421 aatcttcttc cgttaccggc gtgccaacag gcgtaacgcg gcggtagatg gagaaaatgg
      481 taccgacaga gacgttaaca tcgatgttgg acgagccatc cagggggtcc atcagcacca
      541 cgtattttgc gtgttcacag ccttcaaaga cgacaatctc atcttcttct tcagaggcaa
      601 tccccgcaac gatatcgcgt gctttcagtg cggctttcag tttttcatta gcgaacaagt
      661 cgagtttttg ctgaacctcg ccctgcacgt tctcagcacc gctggcaccc aggatatcaa
      721 ccagtcctgc tttgttgata tcgcgatgga taatcttggc gcccagtttt attgccgaca
      781 gcaaagcagt gagctcaccg gtagcatgag aaaactcgtg ctgcttttcg acaataaatt
      841 cacctaacgt tttcataaaa ctttccctgc aatgtttatg gagtaaagcg accgcaacaa
      901 tcttaacaaa taatctcaat gttgcgctca ggtgaatcgc gccagcaaat tgcggattat
      961 cctgaaatgc gtttctcact tgcccgacat atgcgtaaaa tgagcggcag attaaaaaag
     1021 gatagtgacg tatgcgcatt catattttag gaatttgtgg cacatttatg ggcggtctgg
     1081 cgatgctggc gcgccagtta ggccatgaag taacgggttc ggacgccaat gtgtatccgc
     1141 cgatgagcac cttacttgag aagcaaggca ttgagctgat tcagggttac gatgccagcc
     1201 agctcgagcc gcagccggat ctggtgatta ttggcaacgc catgacccgt ggcaatccgt
     1261 gtgtggaagc ggtactggaa aaaaacatcc cttatatgtc aggtccacag tggctgcacg
     1321 attttgtgct gcgcgaccgc tgggtgctgg ccgttgccgg tacacacggc aaaaccacca
     1381 ccgcgggaat ggcgacctgg attctggaac agtgtggtta caaaccggga tttgtgatcg
     1441 gcggtgtgcc ggggaacttt gaggtttcgg cgcgtctggg cgaaagcgac ttctttgtta
     1501 tcgaagcgga tgagtacgac tgcgccttct tcgacaaacg ctctaaattt gtccattact
     1561 gcccgcgtac gctgatcctc aacaaccttg agttcgatca cgccgatatc tttgacgacc
     1621 tgaaagcgat ccagaaacag ttccaccatc tggtgcgtat cgttccgggg cagggccgta
     1681 ttatctggcc ggaaaatgac atcaacctga aacagaccat ggcgatgggc tgctggagcg
     1741 agcaggagct ggtgggtgag cagggtcact ggcaggcgaa aaagctgacc accgatgctt
     1801 ccgaatggga agttttgctg gatggcgaaa aagtgggcga agtgaaatgg tcgctggtag
     1861 gcgaacataa tatgcacaat ggcctgatgg cgattgcagc ggctcgccat gttggtgtag
     1921 cgccggcaga tgccgctaac gcgctgggtt cgtttattaa tgctcgtcgc cgtctggagt
     1981 tgcgtggtga agcgaatggc gtcacggtat atgacgattt tgcccatcac ccgacggcga
     2041 ttctggcaac gctggcggcg ctgcgtggca aagttggtgg tacggcgcgc attattgctg
     2101 tgctggaacc gcgctcgaat accatgaaaa tggggatctg caaagacgat ctggcacctt
     2161 cattaggtcg tgctgatgaa gtcttcctgc tgcaaccggc gcatattccg tggcaggtgg
     2221 cagaagtggc agaagcctgc gttcagcctg cacactggag tggcgatgtg gatacgctgg
     2281 cagatatggt ggtgaaaacc gctcagcctg gcgaccatat tctggtgatg agcaacggcg
     2341 gttttggtgg gatccatcag aaactgctgg atggtctggc gaagaaggcg gaagctgcgc
     2401 agtaattcgg cttcagcctg atatagcatt gccggataag gcgtttacgc cgcatccggc
     2461 aaaccatact cacatcaaca acgaaaatta cccttcattc tccgccagtt cgcgcagata
     2521 ctggaaaatc tggcgtgcgg atttcggcgg cttattccct tctttctctt tcttcgcgtt
     2581 gcggatcagt gtacgcagtt gctgacgatc ggcatcgggc cacagattca atacttcagc
     2641 gatggcgtca tcaccttgat cgatcagacg atcgcgcagg ttttccagtt tatggaacag
     2701 caccacctgc tggttgtgac ggtttttcag cttatccagc gcctgacgaa taggctctac
     2761 gtcgcgctgg cgcagcatct taccgatgag ctgcaactgg cggcggcgac cttccatctt
     2821 aatacgctgg gcgagctcaa tagccgggcg cagatccgca tctaacggga tcttatccag
     2881 cgcgtttttc cccagatcaa caatttccgc gccaaggcgt tttagctcct cggcatcacg
     2941 tttaatttca cttttactga cccagataat ttcatcgtct tcgtcttcga tgtcatcacc
     3001 gggaacgtcg tcgagccagt cttcgggctg cttagtcatc tcaggctcct taaaaaaaga
     3061 ggctaatgtt accagttaag atgcgcactg aaaaacggtt ctctgttaga cttcagagaa
     3121 actctctaca ttatggcact tgcaatgaaa gtaatctctc aagttgaagc gcagcgcaag
     3181 attctggaag aagcagtttc cactgcgctg gagttggcct caggcaaatc ggacggtgcg
     3241 gaagttgccg tcagcaagac caccggcatt agcgtaagca cgcgttatgg tgaagtggag
     3301 aatgttgaat tcaatagtga tggcgcgctg gggatcactg tttatcacca gaaccgtaaa
     3361 ggtagcgcat cgtctaccga tttaagcccg caggccattg cccgcaccgt acaggcggcg
     3421 ctggatattg cccgttatac ctcgccagat ccctgtgccg gcgtggcaga caaagagctg
     3481 ctggcctttg acgcaccgaa tctcgacttg ttccaccctg cggaagtttc cccggatgaa
     3541 gccattgaac tggcggcccg cgcagaacag gcggcattgc aggcggacaa acgcatcacc
     3601 aataccgaag gtggcagctt taacagccac tacggtgtca aagtttttgg caacagccac
     3661 ggcatgttgc agggttactg ctcaacgcgt cattcgctct ccagctgtgt aattgccgaa
     3721 gaaaatggcg atatggagcg tgattacgcc tacaccattg gccgcgcgat gagcgatctg
     3781 caaacgccag agtgggtcgg ggccgactgt gctcgccgta ctttatcgcg cctgtcaccg
     3841 cgtaaactct ccaccatgaa agcgccggtc atttttgcca atgaagtggc aaccgggctt
     3901 tttggccatc tggtgggggc gatagcgggt ggctcggttt accgtaaatc tacctttctg
     3961 ctggattcgc tgggtaaaca aattctgccg gactggctga ccattgaaga gcatccgcat
     4021 ctgctgaaag ggctggcgtc gacgccattc gacagcgaag gtgtgcgcac cgagcgtcgc
     4081 gatattatta aagatggcat cctgactcag tggctgctga ccagctactc ggcgcggaaa
     4141 ctggggctga aaagcaccgg acatgcgggc ggtattcaca actggcggat tgccggacaa
     4201 gggctaagct tcgaacaaat gctcaaagag atgggcaccg ggctggtggt gacggagctg
     4261 atgggccagg gcgtgagtgc cattaccggt gattattccc gtggtgcagc gggcttctgg
     4321 gtagagaatg gcgaaattca gtatcccgtt agtgaaatca ccatcgcagg taatttaaaa
     4381 gatatgtggc gcaatattgt caccgtcggt aacgatattg aaacacgcag taatatacag
     4441 tgtggttctg tgctgttgcc ggagatgaaa atcgccgggc agtaaaaaac tggcgcgacc
     4501 tgccgcgcta taaactaaaa aattacacaa aatcattcgc actgcatcga gacggaaagt
     4561 gaatgagggc cggggagcgt actcgcagta cgtgacccgg gcgaatgagc gtagccgtcg
     4621 atgaggcagt gtgaaggatg aagtgtaaat aaaaaaggaa gtgagcaatg cgtaaaagcc
     4681 tgttagctat tcttgcagtc tcctcgttgg tattcagttc tgcgtcgttt gccgctgatc
     4741 ttgaagacaa tatggaaacc ctcaacgaca atttaaaagt gatcgaaaaa gcggataacg
     4801 cggcgcaagt caaagacgcg ttaacgaaga tgcgcgccgc agcgctggat gcgcaaaaag
     4861 caacgccgcc gaagctcgaa gataaatcac cggacagccc ggaaatgaaa gatttccgcc
     4921 acggtttcga cattctggtc ggtcagattg acgacgcgct gaagctggca aatgaaggta
     4981 aaaagaagcg caggctgctg cagagcaact gaaaacgacc cgcaacgcct atcaccagaa
     5041 gtatcgttaa ttcctcattt ccctgttgcc tgcactcagg taacagggaa cttatcatcg
     5101 caaatgatgc accacctgat tgctgctgcc gcgccagatc agggaagggt cttttaaatc
     5161 ctgcacaaat ttgccgtcga ccagcacgtt aatcaaatca acaacctgca tttgcgcagc
     5221 gttgagttcg tcgagtttat agcctgtcca cacccagatg tctttacccg gacactcggc
     5281 gcggatgcgt tttaccagtt tcagaatatc cggcacgttt tgcggatgca gcggatcgcc
     5341 gccggagagg gaaatcccct ggcgtttgat acgagtgtca ttcagatctt taatgatctt
     5401 gtcttccatt gctttggtaa atggctgacc agaatttacc cgccatgtgc ttttgttata
     5461 gcaaccgggg cattcatgaa cacacccgga gacaaacagg gtgcagcgag tgccggggcc
     5521 gttgacgatg tcgacaggat agtactgatg ataattcatt atttcgcctg gcttatcagt
     5581 ccgcgtgtag gctgcatccg gtaatcaatg cctgatgcga cgctgtcgcg tcttatcagg
     5641 cctacagccg ttgccaaatg taggccggat aaggcgttta cgccgcatcc ggcatttgct
     5701 tagcgcagag tgaagattaa cctatctgcc cattccccaa atgtttaacg cggcgcttaa
     5761 cttcttcctg cttaccagcg ttaaacggac gtgcatccgg gctacctaaa tatccgcaca
     5821 cgcggcgagt taccgacaca cgggaggcgt catggttacc acatttcggg caagtgaagc
     5881 ctttgctggt gcactcgaac tcaccggtaa agccacactc gtagcactca tcaatcggtg
     5941 tattggtgcc gtaatacggt acatgctgat agctgtaatc ccagacatct tccagcgctt
     6001 tcaggttgtg ctgaatgttt ggatactcgc cgtagcaaat gaaaccaccg ttcgccagcg
     6061 gcgggtaagg cgcttcaaag tcgatcttgt cgtacgggtt caccttcttc tccacatcaa
     6121 ggtggaaact gttggtgtag taacctttat cggtcacgcc cggcaccacg ccaaactcag
     6181 cagtatcgag acggcagaag cggtcgcaca ggttttcact cggcgtgctg taaagactga
     6241 aaccataacc cgtttcttct ttccactgat ccactgcctg acgcagacgt tcaacaatgg
     6301 cgataccttt cgcgcgaagc ttctcgttgt cgtaaacatg ctcgccgctg aacagcgcgt
     6361 taatggtttc gtggatgccg atgtaaccca gcgaaataga cgcacgaccg tttttgaaga
     6421 tttcagaaac atcatcgtca gcattcagac gtacgccaca agcaccttcc atatagagga
     6481 tcggggcgac gcgcgctttc acgccttcga gacgagcgat acgggtcatc agtgccttac
     6541 gtgccagcac cagacgttca tccagcagct tccagaaggt ggcttcatcg ccttttgctt
     6601 ccagagcaat acgcggcagg ttcaggctga tcacgccgag gttgttacga ccatcgtgga
     6661 tctgctcgcc gttttcattt tcccacacgc cgaggaagct gcggcagccc atcggggttt
     6721 taaacgaacc ggtgacgttc actacctgat cgtagttcag gatatccgga tacatgcgct
     6781 tgcttgcgca ctccagcgcc agctgtttga tgtcgtagtt cggatcgcct tttttatggt
     6841 tcaggccatc gcgaatcgca aacaccagtt tcgggaacac cgcagtttta cggtttttac
     6901 cgaggcctgc gatacggtta cgcaggatcg attcctgaat caggcgcgat tcccagctgg
     6961 tgcccagacc aaaaccaaag gttacaaacg gcgtctgacc gttggcggtg tgcagggtgt
     7021 ttacttcgta ctccagcgac tggaaggcat cgtagcactc tttgatggta cgagagttag
     7081 cgtagccttc ggcgtccggg atgttccact cttctgcggt cttgcgatgt ttgttgtagc
     7141 tggcggtaac aaacggcgcc agcacttcat cgatacggtt aatggtggtg ccgccataaa
     7201 tatggctggc gacctgagca ataatctgcg cagttaccgc ggttgccgta gagatcgact
     7261 tcggcggttc aatctcggcg ttccccattt taaaaccctg ggtcagcatg cctttcaggt
     7321 cgatcaacat gcagttgaac atcgggaaga acggcgagta atcgagatcg tgatagtgaa
     7381 tatcgccacg ctcatgtgcc tgcaccacgt cacgcggcag caggtgctgg cgtgcatagt
     7441 gtttagccac gatcccggcc agcaggtcgc gctgggttgg aatcaccttg ctgtctttgt
     7501 tggcgttttc gttgagtaac gaggcgttgg tctgctcgac cagaccacgg atctcctggt
     7561 tcaggcgacc gcgtttttca cgttcaatgt cgcgatcgtg acggtactcg atgtaagcac
     7621 gagccagttg tttgtatgga cccgacatca gctggttttc aactgcggtc tggatctcgt
     7681 tgatatccac ctggttgcgc ccctgcatct gctcgttgac aaccgcggca acagtggcgc
     7741 aataatcggc atcatcgact tccgctgctt tagctgcacg cagaatcgct tctttgatgc
     7801 gctctgattt aaacggcact ttgcagccgt ctcgtttcat cacatgcggt gtcatgatcg
     7861 ctccatattt ttaagaacag gttatccaca gaaattggga aaggcgtttt ccggtttttt
     7921 cgtttcgttt tccggtgctt tccgcaatcg ccatccgctt atccacaacg ccgcaccttt
     7981 ttcgtgggga gctgtagtag caattatagt cgattaatac aacatattgg gttgggacgc
     8041 attttaaagt ctatatatag tgctttgcat caaggatgtt ttaacttttt ttgatgtagc
     8101 tcaaagtaaa aagcagagcg tacggatgac gggcgctaca gcgttatgta aattttttaa
     8161 tgaatttgct ggttgaaaaa tcaacaaaaa caacatgctg acagacaaaa ccccggaatg
     8221 accggcatta tcggggctta gggaagattt acttctgtaa ccaccagaca gcctcaaaag
     8281 gccgtaaatt catggcacag ggttgtggtg aggcttcttc gtagttatgc atcacaagct
     8341 gccagttgcc gcgcatttgc cctggctgcc agggttggat ctcacggcta aggttggcaa
     8401 tgaccagcaa ggtttgcccc ttccattcac ggcgatagca ccacaataca gggctgtttg
     8461 gcagcagatc ctggtaattg ccccatgtca ggatggcttc ttgcttacgc agtgcgatta
     8521 acttttggta ggtgtaaaac acagaggagt catcggccag cgcggcttct acgttgattt
     8581 gttgatagtt atcgcctagg ccaatccacg gttcgccagc cgtaaacccg gcattatcgc
     8641 cgttgctcca ttgcatgggc gtgcggctgt tgtcacggga tttactggcg aggattgcca
     8701 ataactcgtc ggcatcacga ccatcgttgc gcagctcggc aaacatattg aggctctcca
     8761 cgtcgcgata gtcagtaatg cgcgtgaaat gcgggttggt catgccaatc tcttcgccct
     8821 ggtagatata cggcgttccc tgcatgccat gcagcaccat cgccagcatt tttgccgcag
     8881 gcacgcggta ttcaccttca tcaccaaagc gagaaacaat gcgcggctga tcgtggttac
     8941 accagaacaa ggcattccat gccacgttgt gcattccttg ttgccagtgg cggaacaatg
     9001 ttttcaacgc cacaaagtca ggtttagcca gcgtccattt ttcaccaccg ggataatcga
     9061 ccttcaggtg atgaaaatta aaggtcatcg acaattcact gcctgtcagt gccgcgtatc
     9121 gctggcaatg ctcaaggctg gtggaggaca tttcacctac ggtcattaac ccgcgtggcg
     9181 taaacacatc gcggttcatc tcgtgcaaaa actcgtgtgc tcgtggcccg tcggtgtaga
     9241 agcgacgccc gtcgccatcc aggtcttcag ggaaacgcgg gtctttggag atcagattca
     9301 ccacatccag gcgcaacccg tcgaccccac gatcggccca gaattcacag acttttttca
     9361 gctctgcgcg taccgctgga ttctcccagt tgagatccgc ctgttctggt gcaaagagat
     9421 gcaaatagta ctgttcgctt tccgcatgcc agcgccacgc actaccgcca aattttgaac
     9481 gccagttgtt cggtggcgtt tctggttcgc catcgcgcca gatataaaac tggcggtaag
     9541 ggctttcttt gttcagcgcc tcgcgaaacc aggcatgttg ggtagaggta tggttaaaca
     9601 ccatatcgag aatgatacga atcccgcgcg attttgcctg cgtcaccagt tcgtcaaaat
     9661 cgtccagcgt gccgtaggtg ggatcaatcg ccgtatagtt cgctacatcg taaccgttat
     9721 cgacctgggg agagacataa aagggggtta gccagatggc atcaacgccc agtttatgca
     9781 gatagtccag gcgttggata acgccacgta aatcgccggt gccgctaccc gtggtgtcct
     9841 gaaaactctt tggataaatc tggtagataa cgccgttttg ccaccagtgg ggaagattag
     9901 tcattacgtt attcctgcaa atgcgagggg gcgcaattgc gccccgaaga aaattaaaca
     9961 atgtccagcg tgcccaggcg gtatttccgc tgatagataa acgaggtgag tacaatcggg
    10021 atgatgatgg cgatagccat tgccagcgca aatacctgcc agtagctcgg ttgaatagag
    10081 agaattcccg gcaggccccc tacgccgata ccattcgcca taacgccgtt caggccgcat
    10141 agcaatcctg ccagaccaga accaatcatc gcgcacagca tcgggaagcg atatttcagg
    10201 ttgatgccgt acattgcagg ctcagtgacc ccaagccagg cggagatagc ggcaggcacg
    10261 gagatctcgc gttcattgtg cttgcggctg gaaatgataa tgcctatcac ggcggagccc
    10321 tgagcgatat tcgacagcgc aatcagcggc cacactggcg tgccacccat gctttgaatc
    10381 atctgcaaat caatagcaag ggtggtctgg tgtacgccgg tgatcaccag cggcgcgtac
    10441 aggaagccaa acaatgccgc accaatcgga gcaaagctgc cggtcatcag gtgacgtacc
    10501 gcaaaggcaa cgccatcgcc aatcatgcga ccaaacggac caatcagcgc atgggcgagg
    10561 aacaccgcga ggatcagcga acagacgggc accaccacca gatagaggta atccggtacg
    10621 atgcgtttaa ggcgagtttc aataacgccc agcgccagcc cggctaacag tgccgggatc
    10681 acctgcgcct gatagcccac tttggcgatg ctgaacatgc caaagtccca cacttccggc
    10741 agctgctgcc cgagcagata agcgttcatc agctgggggg aaaccagtgt cacgccaagc
    10801 acgataccaa ggatcggcgt gccgcccatt tttttcaccg ctgaccagca aatcccgacc
    10861 ggcaggtaga agaaaatcgc ttcaccgatc aaccacagaa aatcgtagat cgtttgcagg
    10921 gaagggtaca tttgcgccag cgtctgaccg ttgctcatgg gcaaatcgcc gatcacattg
    10981 cgaaaaccga ggatcaaacc gccgctaatc aacgcgggca gcaacgggaa gaagatctcc
    11041 gcgaaatgag agatcaactg ctcatgccat ttcatatttt gccgggcggc tttttttacc
    11101 tgctctttat caacctgcgc ctgtccggtt gacgctatta gtgcttgata gtaatcaccc
    11161 acgttggtgc caatcaccac ctgaaattgc ccggcattgg tgaaacagcc tttcaccatg
    11221 gggagttgct caatttcttt cggtctggca ttggccggtt ggttgaggac aaagcgcagg
    11281 cgagtaatac agtggctcac cgtcgcaata ttgccgcgcc cgccgaccag ttcaatcaac
    11341 cgatcgatat ccgtttggtt tattttgctc atcataaagc cccatggcag atgacatttt
    11401 tggttggctg cagaatatat tgcgcggaaa aatttaaaaa cgggaacgtt cccgaaacgc
    11461 agcgaagatc acaatttatc gttcaggaaa cgatcaggac agggtggcgg ggatgatgat
    11521 ttgttgcggt tcgctgcgcc cggttacctg tgcgatcaat tggcaggccg cctggcgtcc
    11581 agcttcggcg tagccaggat ctacggtgac gatctccgga tgaaggaatt tcattaacgg
    11641 cgtattacct acgctcgcca gttgcaaggt gtcgatgcgt tgctcttgca ggtatttact
    11701 tgcgccaaga gccagcgtgt cggttgcgca cagtaacgcg gtagtttcag gcgtaatcac
    11761 ttttgctacg ttttcatagc cttgcttcat agcaagccct ggcagagcgg caacgggatg
    11821 cagtttatgc gctttgcaga acgccaggta ggcttcgtga cgtcgcttcc cggtcgtcac
    11881 gtcactatgc ggcacgccga gataactgat attgcgatgc ccctggtcat agagccgttg
    11941 catcagaatc ttgattgccc cttcgtcgtc ataacagacc gaagcaaagc cttttgcgtc
    12001 acgcgccagc agaaccagcg atgactgcca gtgggctaac atttcttcgg ttatgccggt
    12061 aaaaccgaac agcactacgc cgtcgatatt acgccgtttc agcaccccca aatgttcggc
    12121 aactaattgt ggggaaaact gattttccat catgattgga tcgtaacctt gttcatagaa
    12181 cgctggcagc atggtttgaa cggcgagatt ttctgacaac gaatccagac gggtaacaat
    12241 gatggcgacc actttatcgc tttgcccacg catagcgcgc gcagagcggg aaggggaaaa
    12301 tccatgctga ttcatcactg cttcaacacg ctcgcgcgtg cgctgactca cgccgctttc
    12361 gttattcagc acccgggaaa ccgtagattt ccccacgccg cttaagcgcg cgatgtcttt
    12421 gatggtcagc cgattttgca tcctgttgtc ccataacgtg ttgtttaatt atttgagcct
    12481 aactttaccc gtgcattcag caatgggtaa agtctggttt atcgttggtt tagttgccag
    12541 caggtattat atcgccataa atgctacgaa tattattgga ttctccttat tatttgcggc
    12601 gcttttttca cttaccggag gttatatgga acctgatccc acgcctctcc ctcgacggag
    12661 attaaaactt ttccggtaag cccgtctttt cacggcgtta ccggatgcgt aaggccgtga
    12721 cgttttaatg tccctgctca gctttattac cttcaggtaa ggcttcgaca cgcctgaaga
    12781 catttctgta ctgtttcaga cagtgcggag ggactcctta tgtttaaaga aatttttacc
    12841 cggttcattc gccatttacc ttcccgtctg gttcatcgcg atccattgcc tggcgcgcag
    12901 cagacagtga atacggcggt cccgccgtcc ttaagtgcgc attgcctgaa aatggcggtg
    12961 atgcccgaag aagaattgtg gaaaacgttc gacacccatc cggaaggatt aaatcaggcg
    13021 gaagtggaat ctgcccgcga acaacatggt gataataaat tacccgccca acaaccgtcg
    13081 ccgtggtggg tacatttatg ggtctgctat cgcaacccct ttaatatttt gctcaccatt
    13141 cttggtgcta tttcttacgc cacggaagat ttatttgccg caggtgttat cgcgctaatg
    13201 gtcgctattt ctacgttgct gaactttatt caggaagctc gttccaccaa agcggcagat
    13261 gccctgaaag cgatggtcag taataccgcg acggtactgc gagtaattaa cgacaaaggc
    13321 gaaaatggct ggctggagat cccgatagac cagctggtgc ccggcgatat tataaaactg
    13381 gcggcggggg atatgatccc ggcagattta cgtatcttgc aggcgcggga tctgttcgtc
    13441 gctcaggcgt cgttaaccgg tgagtctctg cccgtggaaa aagccgctac tactcgccag
    13501 ccggagcaca gcaatccgct ggagtgcgac accctgtgtt ttatgggcac caccgtggtg
    13561 agcggcacgg cacaagcaat ggtgattgct acaggagcca acacctggtt tggtcaactg
    13621 gcggggcgtg ttagtgagca ggaaagcgag ccgaatgcct ttcagcaagg gatcagccgc
    13681 gttagtatgc tgctgattcg ctttatgctg gtgatggctc cggtggtgtt gcttatcaac
    13741 ggttacacca aaggcgactg gtgggaagcg gcactgtttg cgctttcggt cgcggtaggc
    13801 ttaacgccgg aaatgttgcc gatgattgtt acctcgacgc tggcgcgcgg cgcagtaaag
    13861 ctgtcgaaac agaaagtgat cgtcaaacat ctggatgcca ttcagaactt tggcgcaatg
    13921 gatattctgt gcactgataa aaccggcacc ctgacgcagg acaaaattgt gctggagaat
    13981 cataccgata tctccggtaa aaccagcgaa cgcgtgctgc atagcgcgtg gttgaacagt
    14041 cattaccaga ccggacttaa aaatctgctt gataccgcgg tgctcgaagg tacggatgaa
    14101 gagtcagcgc gttcgctggc cagccgttgg caaaaaattg atgagattcc gtttgatttc
    14161 gagcgtcgtc ggatgtcggt ggtagtggca gaaaataccg aacaccatca gctggtttgc
    14221 aaaggtgcat tgcaggaaat cctcaatgtg tgttcgcagg tgcgtcacaa tggcgagatt
    14281 gtaccgctcg atgacaccat gctgcgtaag attaagcggg ttactgatac gctgaatcgt
    14341 caggggctgc gcgtggttgc ggtggcgacg aaatacctgc cagcgcgtga aggagattac
    14401 cagcgggcgg atgaatccga cctgatcctc gaaggatata ttgctttcct tgatccgcca
    14461 aaagagacaa ccgctccggc actgaaggca ttaaaagcga gtggaattac cgtaaaaatc
    14521 cttactggcg acagtgagtt agttgcagcg aaagtgtgcc atgaagtggg gctggatgcg
    14581 ggagaggtgg tcattggtag tgatattgaa acgctatctg acgatgaact ggcaaatctc
    14641 gcacagcgca ccacgctgtt tgcccgcctg acgccgatgc ataaagaacg cattgtgact
    14701 ttactgaagc gcgaagggca tgtggttggc tttatgggcg atggtattaa tgatgcgccc
    14761 gccttacgcg ctgcggatat cggcatttct gtggacggcg cggtagatat tgcccgtgaa
    14821 gcggctgata tcatcctgct ggaaaaaagc ctgatggtgc tggaagaggg ggttattgag
    14881 ggacgtcgca ctttcgccaa catgctgaaa tacatcaaat tgacggcgag ttctaacttc
    14941 ggtaatgtgt tcagcgtgct ggtggcgagt gctttcttgc ccttcctgcc gatgttgccg
    15001 ttacacttgc ttattcagaa cctgctgtac gatgtgtcac aggtggcgat cccgtttgat
    15061 aacgtcgacg acgagcaaat tcaaaagccg cagcgttgga atccggcgga tctggggcgc
    15121 tttatggtct tcttcggacc gatcagctcg attttcgata ttctgacgtt ttgcttgatg
    15181 tggtgggtat tccatgccaa cacgccggaa acgcaaacgc tgttccagtc gggctggttt
    15241 gtggtgggat tactgtcgca aacgctgatt gtgcatatga tccgtacccg ccgtgtgccg
    15301 tttattcaga gctgtgcatc gtggccgtta atgatcatga ccgtgatcgt gatgattgtc
    15361 gggatcgcat tgccgttttc accgctggcc agttatctgc agctgcaggc gctgccgtta
    15421 agctatttcc cgtggctggt tgcgattctg gcagggtata tgacgttaac ccagttggtg
    15481 aaagggttct atagccgtcg ttacggctgg cagtaaagaa taaactgggc atggtggccc
    15541 ggattcagat tgcagacaaa gtgagctttg ttcatgccgg atgcggcgta aacgcccaat
    15601 ccggcctaca aattcaataa attgcaggaa tcacgtaggc ctgataagcg tagcgcatcg
    15661 ggcgattttg cttttgtcat cagacttaat ccgggcatga tagcccggat ttccatcaag
    15721 attagcgacg aacagcgatt gcttcgatct caatcttcac gtctttcggc agacgggcaa
    15781 cttcaacgca agaacgtgcc gggaaagtgg cgttgtgttc ggtgaagaag gcttcgtaag
    15841 tggcgtttac ggttgcgaag tcgttcagat ctttcacgaa cacagtggtt ttaacgatgt
    15901 cgcccacttt caggccagcg gcttcgacga ttgcttttac attatccagc gactgacgtg
    15961 cctgtgcagc gacgtctgcc ggtacttcac cagttttcgg atttaccggg atctgaccgg
    16021 aggtgatgat catattgccc agatcaacgc cctgtacgta aggaccgata gctgccggtg
    16081 cattttccgt cgcgatagtt ttgctcatga tttctccttt attacagcgg taaaaagtct
    16141 ggctcattat agggagccag acttttatta ccaaccgcaa ttaattggcc agcaccacat
    16201 tatgggaaaa ctctttttca cagtatttgc atttaagcgc gatgtcattg gcgcgttttc
    16261 gcacggcaaa gctggatgaa accggttcgg catggctgat acagttgctg ttcgggcaga
    16321 ccagcacatt gtcgatgcgc tctggcagac ttgggcgcga tttacccacc acttcatagt
    16381 tgtcgatacg gttaaccgtg gcttgcggtg catacaatgc cagttggtct acttgctctt
    16441 cactcaaaaa ggtattttcg attttgatca gatctttgcg gcccatctcg ccagaaggca
    16501 aattcagacc aatggtgatg cgctgatctg tttcggtcaa cttgaacaga ctcaacagct
    16561 taaaaccgat ctgggcgggg atatggtcaa ttaccgtgcc gcgtttaata gcttcaacct
    16621 gcaatttatt atcgtgtgtc atctctattt ccccttacag taccagatcg cgattcagaa
    16681 ccagtgccag taacgcctgg cgagcgaaaa tcccgttgcc tgcttgctgg aagtaccagg
    16741 cgtgaggcgt tttatccaca tccgtcgcaa tctcatcaac acgcggcagc ggatgcagca
    16801 ctttcatatt ggctttggcg ttgtggagat cgctggcgcg aagaacaaac tgcgctttca
    16861 cgttggcgta ctcggacggg tccagacgct ctttttgcac gcgggtcatg tacaggatgt
    16921 ctacttccgc catcacttct tcaatagagc tgtgcagact ccaggcgatc cctttttcat
    16981 cgagcatatc cagaatgtat tgcggcattg ccagcgcatc cggcgcgata aagtaaaaac
    17041 ggttgccgtc gaacttcgct agcgcctggg tcagggagtg aacggtgcgg ccatatttca
    17101 ggtcaccaac cattgcgacg tggagattgt ccagacgccc ctgggtttcc tgaatggtga
    17161 ataagtccag caaggtttgc gtcggatgtt ggttggagcc atcaccggca ttcagtaccg
    17221 gtacattgcc agaaaattcg gtggctaggc gtgccgcacc ttcctgcgga tgacgcatca
    17281 ctatcgcatc gacgtaagtg ctgataaccg aaatggtatc ggccagcgtt tcgccttttt
    17341 tacccagtga tgtattggcg ctgtcggaga agcccaccac gctggcaccc aggcggtgca
    17401 tggaagtttc gaaagagagg cgggtacggg tagaggcttc gaagaaacag ctggcaatga
    17461 ctttgtgctt caacagctct ggttgcgggt ttgctttcag tttcgccgct gtcgccagca
    17521 ccagattaag gtcatcgcga ctaaggtcgt ttatggaaat gatatgtttc tgatatagcg
    17581 gattagccat cttttatctc ctgacaccta ggcaaaaaaa agcccctcga ttgaggggct
    17641 gggaatgggt gatcaacggg aagaaaaacg gcaggccagc gtcttttttc agacgcggta
    17701 agacaaaatg tcgaacacac tgaaccatac atcctcccgg caaattgtcc ggcattatac
    17761 tcatagccag aagcggtgca agcatttgat gcaatatttt gtcagcgcaa acggtttatt
    17821 tgaattaaaa gtcaaaatat atgcattttt attttctatg attctggagg ggaagtgtcg
    17881 ttcggtatca ataattgcag acatttcctg cttttttctt tttcattttc acgcaatcag
    17941 aaatgcattt tattccattc atccgactta tagggcggag ggtgaaaacg aacggctaac
    18001 actattgctt atagctcagg gatacgcgct atcactttaa tttcaaaatc aaagcctgcc
    18061 agccaggtaa cacccaccgc cgtccagttt ggataaggtg gggcgctaaa tatttcattt
    18121 ttcaccgtca tgatgtcttc agattgtttt tccggatcgg tatgaaagct cgtaacatca
    18181 atgatatcgt caaaagtgca tcccgcagct gccagggtcg catgcaaatt atcaaaggcc
    18241 agtctgactt gttgctgaaa atcgggttct ggtgttccgt cctctcgact tcctacttgc
    18301 ccggaaacaa acagcaaatc gccggaacga atagccgcag aataacgatg ctcagcatac
    18361 agtgaatgtc ggccagcagg gaaaacagcg gttctttcta ccatttggtt atcctcaaga
    18421 tttacgacat gaacagaaga tttatattta tcgggagccg cttttagggg gacgacgtta
    18481 gtaaacaaaa cccagacatc atggataatg gctgggctta attgagcgta gtcggttatg
    18541 cgccaaacgc gccatcaatg gtatgcatcg cgccggtaac aaaactggct tctggccctg
    18601 ctaaccatgc gaccatacca gcgacctctt ccggttgccc atgtcttttg atggccataa
    18661 aactatgcaa catatcgcgc attggcccgt tggcgggatt aacgtcggta tcaattggtc
    18721 ctggctggac gacgttgatg gtgatccctc gcggtccaaa atcacgggcc agcccgcgcg
    18781 ccatgccttg cagggcagat ttactggcgg cataagcagc catgcctgca acaggcatac
    18841 gatcgccatt cacggagccg atgattaaga tgcgcccgcc ttcgggcatc tgccgggcgg
    18901 cttcaacaga ggcatgataa ggagcatgaa tattgatttt gaaaaggcga tcaatatcgt
    18961 cggcatttaa ttccagggca tcgccaaaga cgccaatacc tgcatttacc accaggatat
    19021 ccaatgcgcc gctcttacga acgacatcaa taacagcgtc tctgtcagca ctatctgtga
    19081 atactgctgt cgctccagtc tcttgtgcca gacgttcagc ggcatctttc gaccccgcat
    19141 aggtgaatcg tacattggcc ccatcggtga cgaaacgacg tacgatagcg gcaccgattc
    19201 cacgactgcc accgaggatg agaactgtct taccggtaaa agcgcccata aggactcctt
    19261 gatttattat gtaacatgca ttacaaaact gttttaactt tctgtcaaca ggttttgtaa
    19321 tggtcactaa aaaacaatct cgcgttccag gtcgtcccag acgtttcgct cctgagcagg
    19381 cagtctctgc ggcaaaagtg ctttttcacc aaaaaggttt cgatgcagta agtgtctctg
    19441 aagttactga ttatcttggt attaaccccc cgagtctcta cgcggctttt ggcagtaagg
    19501 ctgggttatt tagccgtgta ctcaatgaat atgtcggtac ggaagctatt ccgcttgccg
    19561 atattcttcg tgatgatcgt ccggtaggcg agtgcctggt tgaggtatta aaagaagcgg
    19621 cgcgcagata tagccaaaac ggcggctgcg ctggctgtat ggttcttgaa ggtattcata
    19681 gtcatgatcc acaagcgcgt gatattgcca ttcaatatta tcacgccgca gaaacgacca
    19741 tttatgacta tatcgccagg cggcatccac aaagcgcaca atgtgtgact gattttatga
    19801 gtaccgtgat gtcagggctt tctgcgaagg cacgggaggg gcactccatc gaacaactct
    19861 gtgcaacagc tgccctggca ggggaagcga taaaaactct tctcaaggag tgatgctggc
    19921 cttgatccga aaggcgggaa cgcgcctgcc gataagttgt gataagacaa taattcacgc
    19981 gttaaggctg gcggaattga tcatcttttc gtataacgat agaaatgaaa cattgtttta
    20041 attaaggagt ggaaaagatg atcatcggaa atattcataa tcttcagccg tggctacccc
    20101 aggagttacg ccaggcgatt gagcatatca aagcacacgt tacggcagaa acgccaaagg
    20161 gcaagcatga tatcgaaggc aatcgtctgt tttatcttat ctcggaagat atgaccgagc
    20221 cgtacgaagc tcgccgtgcg gagtaccatg cccgctatct tgacattcag attgtgttaa
    20281 aaggtcagga aggcatgacc ttcagcacgc aacctgcagg cgcgccggat accgactggt
    20341 tagctgataa agacatcgca tttttgccgg aaggcgttga tgagaaaaca gttatcctaa
    20401 atgaaggtga ttttgttgtg ttttatccgg gggaagtgca taaaccgctg tgcgcagtgg
    20461 gcgcgccagc ccaggttcgc aaagcagtag tgaagatgct gatggcgtga tgacttttcg
    20521 ccgtaaataa ctccaggt
//
LOCUS       DAJTYM010000069        20431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTYM010000069 DAJTYM010000000
VERSION     DAJTYM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..214
                     /locus_tag="OO575_002628"
     CDS             <1..214
                     /locus_tag="OO575_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6665542.1"
                     /translation="LCMLSGSPGTSAGELTRITGLSASATSQHLARMRDEGLINSQRD
                     AQRILYSIKNEAVNAIIATLKNVYCP"
     gene            224..748
                     /gene="ygaP"
                     /locus_tag="OO575_002629"
     CDS             224..748
                     /gene="ygaP"
                     /locus_tag="OO575_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6665543.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(795..1199)
                     /gene="stpA"
                     /locus_tag="OO575_002630"
     CDS             complement(795..1199)
                     /gene="stpA"
                     /locus_tag="OO575_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6665544.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            1867..2316
                     /gene="alaE"
                     /locus_tag="OO575_002631"
     CDS             1867..2316
                     /gene="alaE"
                     /locus_tag="OO575_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6665545.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2353..2697)
                     /locus_tag="OO575_002632"
     CDS             complement(2353..2697)
                     /locus_tag="OO575_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6665546.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            2849..3079
                     /locus_tag="OO575_002633"
     CDS             2849..3079
                     /locus_tag="OO575_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665547.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3426..3671
                     /gene="nrdH"
                     /locus_tag="OO575_002634"
     CDS             3426..3671
                     /gene="nrdH"
                     /locus_tag="OO575_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6665548.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3668..4078
                     /gene="nrdI"
                     /locus_tag="OO575_002635"
     CDS             3668..4078
                     /gene="nrdI"
                     /locus_tag="OO575_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6665549.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4051..6195
                     /gene="nrdE"
                     /locus_tag="OO575_002636"
     CDS             4051..6195
                     /gene="nrdE"
                     /locus_tag="OO575_002636"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6665550.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6205..7164
                     /gene="nrdF"
                     /locus_tag="OO575_002637"
     CDS             6205..7164
                     /gene="nrdF"
                     /locus_tag="OO575_002637"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6665551.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7519..8721
                     /gene="proV"
                     /locus_tag="OO575_002638"
     CDS             7519..8721
                     /gene="proV"
                     /locus_tag="OO575_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6665552.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8714..9778
                     /gene="proW"
                     /locus_tag="OO575_002639"
     CDS             8714..9778
                     /gene="proW"
                     /locus_tag="OO575_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6665553.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9836..10828
                     /gene="proX"
                     /locus_tag="OO575_002640"
     CDS             9836..10828
                     /gene="proX"
                     /locus_tag="OO575_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6665554.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11020..12199
                     /locus_tag="OO575_002641"
                     /pseudo
     CDS             11020..12199
                     /locus_tag="OO575_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12323..13060
                     /gene="ygaZ"
                     /locus_tag="OO575_002642"
     CDS             12323..13060
                     /gene="ygaZ"
                     /locus_tag="OO575_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6665555.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13050..13385
                     /gene="ygaH"
                     /locus_tag="OO575_002643"
     CDS             13050..13385
                     /gene="ygaH"
                     /locus_tag="OO575_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6665556.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13476..14006
                     /gene="emrR"
                     /locus_tag="OO575_002644"
     CDS             13476..14006
                     /gene="emrR"
                     /locus_tag="OO575_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6665557.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14133..15305
                     /gene="emrA"
                     /locus_tag="OO575_002645"
                     /pseudo
     CDS             14133..15305
                     /gene="emrA"
                     /locus_tag="OO575_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15322..16860
                     /gene="emrB"
                     /locus_tag="OO575_002646"
     CDS             15322..16860
                     /gene="emrB"
                     /locus_tag="OO575_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6665558.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(16924..17439)
                     /gene="luxS"
                     /locus_tag="OO575_002647"
     CDS             complement(16924..17439)
                     /gene="luxS"
                     /locus_tag="OO575_002647"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6665559.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17589..19145)
                     /gene="gshA"
                     /locus_tag="OO575_002648"
     CDS             complement(17589..19145)
                     /gene="gshA"
                     /locus_tag="OO575_002648"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6665560.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19218..19646)
                     /locus_tag="OO575_002649"
     CDS             complement(19218..19646)
                     /locus_tag="OO575_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6665561.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19643..20209)
                     /gene="yqaB"
                     /locus_tag="OO575_002650"
     CDS             complement(19643..20209)
                     /gene="yqaB"
                     /locus_tag="OO575_002650"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6665562.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4921 a         5237 c         5112 g         5161 t
ORIGIN      
        1 tctgtgcatg cttagcggtt cccccggcac cagcgcggga gagctgacgc gcattaccgg
       61 actgagcgcc tctgcgacat cacagcatct cgctcgtatg cgggacgaag ggcttatcaa
      121 cagccaacgg gatgcccaac gcattctata ttccattaaa aatgaggcgg taaatgccat
      181 tatcgccacc ctgaaaaatg tctattgtcc gtaaggagtc actatggctt tgacaaccat
      241 ttcgccgcat gatgcacaag aattaatcgc acgcggcgca aagttaatcg atattcgtga
      301 cgctgatgaa tatcttcgtg aacatattcc tgaagcagat ctggctccat tatccgtgct
      361 ggaacagtca ggtcttccac ctaaattacg tcgcgagcaa attatattcc actgccaggc
      421 aggtaaacgc accagtaata acgccgataa attagcagcg attgccgccc ccgcagaaat
      481 atttttactc gaagatggga ttgatggctg gaaaagagct ggattgccag tagcggtaaa
      541 taaatctcaa cccttgccgt taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt
      601 aaacggcgtt gtactgggtt ataccgtaaa tagcggtttc ttcttattaa gtggctttgt
      661 tggtgccggg ttactgtttg caggaatcag cggtttttgt ggaatggcaa ggttgttaga
      721 taagatgccg tggaaccaac gagcttgaga agcgacgccg gacgcgccct agcagcgaca
      781 tccggcctca gtaattagat caggaaatcg tcgagagatt taccttctgc cagcgcctga
      841 gcaattggct tcggtgtacg gccctgaccg gtccaggttt tagtttcacc gttaacatca
      901 gtgaatttat atttcgccgg acgcggctgg cgttttttac cagcacgtgg tgcagcagca
      961 gagctattac ccaataactc ttccgggtta attccgtcag ctttcatcag ctccagccag
     1021 gtgctaattt tttcctgacg ctcagccagt tcacgctgct gctgttcttc ttcttcacgt
     1081 ctttctttag tgacaaccct gaatttttcg agcatttctt caagaacgtc aatggagaat
     1141 tcgcgagcca tcgcacggag ggtgcgaatg ttatttaaac tttgtaacat tacggacata
     1201 ataaagaaaa ccttttaacg ccaaaacaaa aaagtatttc gtcgcgtagt gtaagcgatc
     1261 acttattata tttccacaaa atttaatcta tttacagtcc tgcgcgagat tatttcagct
     1321 gttattttct gaaactctca gaataatttc aagattatat attagccatt ttgatgagta
     1381 atttaggtta ttggtgaaca gagtggcgct ctctgctcgc taactccgca agcctgacaa
     1441 aaccgaagtg aataaaaatg caatataaca gaataaaaga aaacacccct cactggtgct
     1501 ttctgctgtt aatgtacatt ttctggcatc aaaaatgctg taaatgaccc ggtcatgaca
     1561 aacaccgaat tctctacgct tatagcgtta cctccccccc aaactttcat tagtgaaatt
     1621 aaaggcaaga tcatatctaa ttgatttaat tgaaaataaa tcatattggc atgtaaaaat
     1681 ccacaatgta caaaaaacga gccgttacgg aatattttat ctacaaaaac taactaaata
     1741 aaaatttttc actaattgat tagtcataga cagcgatata cgctatgcaa aaatgcagat
     1801 ggcaatgaga tccactgctt tcatctccat taacatccca ttacgctttt attaaggagc
     1861 attagcatgt tctcaccgca gtcacgcttg cgtcatgcag ttgcagatac gttcgcgatg
     1921 gttgtttact gttctgtcgt gaacatgtgt attgaagttt tcctctccgg aatgagcttc
     1981 gaacagtctt tttattccag attggtagcg attccggtga acatcttaat tgcatggcca
     2041 tacggtatgt accgcgatct gtttatgcgc gcggcacgca aagttagccc gtcgggctgg
     2101 ataaaaaatc tggctgatat cctggcttat gtgacgttcc agtcaccggt gtatgtggcg
     2161 atcttgttag tggtgggcgc agactggcat cagattatgg cggcggtcag ttcaaacatc
     2221 gttgtttcga tgttgatggg ggcggtttat ggctacttcc tcgattattg ccgccgactg
     2281 tttaaagtca gccgttacca gcaggtaaaa gcctgactct tcttttcgcg actggcatcg
     2341 ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc aaataacgtt caagcgcaac
     2401 acgcgaacta aagccatgcg ggataccgta atgctcgtgt cgctcgcctg ctaaatagag
     2461 cggaaattgc tggtaatgat cgcaagtttt aatatcggaa gcgggttcag ccagtgttga
     2521 actgcgttct tttaatgttg gatgaattac cagagcggta cgtcccattc gcgcttcgcg
     2581 attaacatag acataatttt ccccacggcg gtaaccatac gctttctggg ttaccacatc
     2641 gacagtaaaa ccgacttttt caagtacgcg cgccacctcg tctggtcgta aatacatatt
     2701 tgatcctcgt tattttcaac cgcgggccta ccttacctga ttgcgcattc acaacgcttt
     2761 cagaaaagtc cataaagccg cgatgaacct cagttaagaa aattatggtc tacactgaaa
     2821 attacatcga attctaatgg gggatcatat gtttaaccga ccgaaccgca acgacgttga
     2881 tgatggcgtg caggatatcc agaatgatgt caatcaatta gctgacagcc tcgaatctgt
     2941 attgaagtcc tggggcagcg acgccaaagg ggaagctgaa gccgcacgca gtaaagctca
     3001 ggcgttgctg aaagaaacca gggcgcgaat gcatggtcgt actcgcgtcc agcaagccgc
     3061 gcgcgatgcc gttggctgag cggattcttt tgttcgtgaa agaccctggt gtagcgtggg
     3121 tacagcagct gcggtaggta tttttatcgg cgcactgtta agcatgcgca aatcgtagtg
     3181 caaaaatgat aataaatacg cgtctttgac cccgaagcct gtcttcgggg tttctttttg
     3241 cctggtgaat cacaaaaatc cccctccccc gtcacgctca tatccagggt aatttcgatc
     3301 actatttgct atatattgtg tagttgaatc ttttttcaac tacatctagt atctctgtat
     3361 caacagagag acaacccgac gcgtatcatc gcgccgtatc ttcattttaa acggaaatac
     3421 gaatcatgcg cataactatt tatactcgta acgattgcgt tcagtgccac gccaccaaac
     3481 gggcgatgga aaaccggggc tttgattttg aaatgattaa tgtcgatcgc gttcctgaag
     3541 cagcggaagc gttgcgtgct cagggctttc gtcagttgcc ggtagtgatt gctggcgatc
     3601 ttagctggtc tggtttccgt ccggacatga ttaaccgtct gcatccagcg ccacacgcgg
     3661 ccagtgcatg agccagctcg tctacttctc cagcagctcc gaaaacacgc agcgttttat
     3721 cgaacgttta ggtctgcccg cggtgcgcat cccgctcaat gagcgggaac ggattcaggt
     3781 agacgagcct tacatcctga tcgtgccctc ttacggcggc ggcggtacgg ctggcgcggt
     3841 gccacgacag gtaattcgct ttttaaacga cgagcacaac cgggcgttgc ttcgcggcgt
     3901 tattgcttct ggtaatcgca actttggtga ggcgtatggc cgcgccggag atgtgattgc
     3961 ccggaaatgc ggcgtgccgt ggctgtaccg ttttgaactc atgggtacgc aaagcgatat
     4021 cgaaaacgtt cgtaaaggag taaccgaatt ttggcaacga caaccgcaga atgcctgacg
     4081 caggaaacga tggattacca cgcgctgaat gcgatgctta atctttacga tagcgcaggt
     4141 cgcattcagt tcgataaaga ccgccaggcc gttgacgcct ttattgcgac gcatgtgcgt
     4201 ccgaacagtg tgaccttcag tagccagcag cagcgcctga actggctggt caacgaaggt
     4261 tactatgatg aaagcgttct taatcgctac tctcgcgact ttgtcattac gctgtttgcc
     4321 cacgcacaca ccagcggttt tcgtttccag acattcctcg gggcatggaa gttttacacc
     4381 agctatacgt tgaagacatt cgacggtaaa cgttatctgg aagattttgc cgatcgagtc
     4441 acgatggtgg cgctgacgct ggcacaaggc gatgagacgc tggcgttgca actgaccgat
     4501 gaaatgctgt caggacgctt tcagccagcc acgccaacat tcctcaactg cggtaagcag
     4561 cagcgcggcg aactggtttc ctgttttttg ctgcgtattg aagacaatat ggagtcgatt
     4621 ggtcgggcgg taaattccgc actgcagctg tcgaaacgcg gcggcggcgt agcatttttg
     4681 ctgtcgaatc tgcgggaagc gggcgcgcca attaaacgta ttgaaaatca atcttctggc
     4741 gtaattccgg tgatgaaaat gttggaagac gcattttcct atgccaacca actcggcgcg
     4801 cgtcaggggg caggtgcagt ctatttacat gctcatcatc ccgatattct gcgttttctc
     4861 gacacgaaac gggaaaatgc cgacgaaaaa atccgcatta aaacactgtc gctgggcgtg
     4921 gtgatcccgg atatcacttt ccatctggca aaagagaatg cgcagatggc gctgttttcg
     4981 ccctatgacg ttgagcgagt ttatggcaag ccgtttgccg atatcgccat tagcgaacac
     5041 tatgacgaac tggttgccga tgaacgcatc cgcaaaaaat acctcaacgc ccgtgatttc
     5101 ttccagcgac tggcagaaat ccagtttgag tccggctatc cctacatcat gtatgaagac
     5161 acagtaaacc gtgctaaccc tatcgccggg cgcataaata tgagtaatct ctgctcagaa
     5221 attttgcagg ttaacagcgc ctcagagtat gacgagaatc tcgactatgc ccgcacaggc
     5281 catgatattt cctgcaattt aggttcgttg aatattgcgc acaccatgga ttcccccgat
     5341 ttttcccgca cggtagagac tgccgtgcgc ggtttaacgg cggtgtcaga tatgagtcat
     5401 atccgcagcg tgccgtccat cgaagccgga aatgccgcct cgcacgccat cggactgggg
     5461 cagatgaatt tacatggtta tctggcgagg gaaggcatcg cttatggttc gccggaagcc
     5521 ctggatttca ccaatctcta tttctatacc atcacctggc acgcactgcg tacctcgatg
     5581 ttgctggcgc gcgaacgcgg tgaaaccttc gccgggttca aacagtcacg ctatgccagt
     5641 ggtgaatatt tcagccaata tctgcaaggt aactggcagc cgaaaacggc gaaagttggc
     5701 gaactgtttg cccgcagctg tattacgtta cctacccgtg agatgtgggc gcaactgcgc
     5761 gacgacgtga tgcgctacgg catatacaac cagaatctcc aggcggtgcc gccaaccggg
     5821 tctatctctt atatcaacca tgctacgtcg agtattcatc cgattgtggc gaaagtagag
     5881 atacgcaaag agggcaaaac aggacgcgtt tactaccctg ccccgtttat gactaacgaa
     5941 aatctggcgc tgtatcagga cgcttacgaa attggcgcag aaaagatcat cgacacctac
     6001 gcggaagcga ctcgccatgt cgatcagggg ctgtcgctga cgctgttttt ccccgatacc
     6061 gccaccactc gcgatatcaa caaagcgcag atttacgcct ggcgcaaagg tatcaaaacg
     6121 ctctattaca tccgcctgcg tcagatggcg ctggaaggca ctgaaattga aggctgcgtc
     6181 tcctgtgcac tttaaggaat atctatgaaa ctctcacgta tcagcgccat caactggaac
     6241 aagatatctg acgataaaga tctggaggtg tggaatcgcc tgaccagcaa tttctggcta
     6301 ccagaaaagg tgccgctgtc gaacgatatt cccgcctggc agacattaac tgtcgtagaa
     6361 caacaactga cgatgcgcgt ttttactggc ctgacgctgc tcgacacgct gcaaaatgtt
     6421 atcggcgcgc cttcgctgat gcccgatgcg ctcacgcctc atgaagaggc ggtattatcg
     6481 aatatcagct ttatggaagc ggttcatgcc cgctcttaca gttcgatttt ctcgacgctg
     6541 tgccagacca aagatgtcga tgccgcctac gcctggagtg aagaaaacgc accgttgcag
     6601 cgaaaagctc agattattca gcaacattat cgcggtgatg atccgctgaa aaagaaaatc
     6661 gccagtgtgt ttcttgaatc ttttttgttc tattccggtt tctggctgcc gatgtatttt
     6721 tccagccgcg gaaagctgac caataccgcg gacctgatcc gtctgattat ccgcgatgaa
     6781 gcagtccacg gttactacat aggctataaa tatcagaaaa acatggaaaa gatatctctg
     6841 ggacaacgtg aagagttgaa gagtttcgcc ttcgatttgt tgctggaact ctacgacaac
     6901 gagttgcaat acaccgatga gctgtacgcc gaaaccccgt gggctgacga tgtgaaagcg
     6961 tttctctgtt acaacaccaa taaggctttg atgaatctgg gctacgaacc gttatttccc
     7021 gcagaaatgg cggaagtgaa tccggcaatc ctcgccgcgc tttcgccgaa tgccgatgaa
     7081 aatcacgatt tcttttccgg ttcaggctcc tcttatgtga tggggaaagc ggttgaaaca
     7141 gaagatgaag actggaattt ctgagggtgt tattttcaaa aatatcacta cccgcagcag
     7201 ggaaataatt cccgccaaat agctttttat cacgcaaata ttctgtagtg atctacactg
     7261 atactctgtt gcattattcg cctgaaacta caatattcag gcgttttttc gctatctatg
     7321 acaaaaaata tcaacttttc tcgatttgct ctcagccctt atatcacgga aaattccggc
     7381 gatttgctcg catcaatatt catgccacat ttgccctcag gggttgcctc agattctcag
     7441 tatgttaggg tagaaaaaag tgactattcc cattgggtaa tatatcgaca taaacaaata
     7501 aagaaatcat tctattgcat ggccattaaa ttaaaaatta aaaatcttta taaaatattt
     7561 ggtgagcatc cacagcgagc gttcaaatat atcgaacaag gactttcaaa agaacaaatt
     7621 ctggaaaaaa ctgggctatc gcttggcgta aaagacgcca gtctggccat tgaagaaggc
     7681 gagatatttg tcatcatggg attatccggc tcgggtaaat ccacaatggt acgccttctc
     7741 aatcgcctga ttgaacccac ccgcgggcaa gtgctgattg atggtgtgga tattgccaaa
     7801 atatccgacg ccgaactccg tgaggtgcgc agaaaaaaga ttgcgatggt cttccagtcc
     7861 tttgccttaa tgccgcatat gaccgtgctg gacaatactg cgttcggtat ggaattggcc
     7921 ggaattaatg ccgaagaacg ccgggaaaaa gcccttgatg cactgcgtca ggtcgggctg
     7981 gaaaattatg cccacagcta cccggatgaa ctctctggcg ggatgcgtca acgtgtggga
     8041 ttagcccgcg cgttagcgat taatccggat atattattaa tggacgaagc cttctcggcg
     8101 ctcgatccat taattcgcac cgagatgcag gatgagctgg taaaattaca ggcgaaacat
     8161 cagcgcacca ttgtctttat ttcccacgat cttgatgaag ccatgcgtat tggcgaccga
     8221 attgccatta tgcaaaatgg tgaagtggta caggtcggca caccggatga aattctcaat
     8281 aatccggcga atgattatgt ccgtaccttc ttccgtggcg ttgatattag tcaggtgttc
     8341 agtgcgaaag atattgcccg ccggacaccg aatggcttaa ttcgtaaaac tcctggcttc
     8401 ggcccacgtt cggcactgaa attattgcag gatgaagatc gcgaatatgg ctacgttatc
     8461 gaacgcggta ataagtttgt cggcgcagtc tccatcgatt cgcttaaaac cgcgttaacg
     8521 cagcagcaag gtcttgatgc ggcgctgatt gatgcgccgt tagcagtcga tgcacaaacg
     8581 cctcttagcg agttgctctc tcatgtcgga caggcaccct gtgcggtgcc cgtggtcgac
     8641 gaggaccaac agtatgtcgg catcatttcg aaaggaatgc tgctgcgcgc tttagatcgt
     8701 gagggggtaa ataatggctg atcaaaataa tccgtgggat accacgccag cggcggacag
     8761 tgccgcgcaa tccgcagacg cctggggtac accgacgact gcaccggctg acggcggtgg
     8821 tgctgactgg ctgaccagta cgcctgcgcc aaacgtcgag cattttaata ttctcgatcc
     8881 gttccataaa acgctgatcc cgctcgacag ttgggtcact gaagggatcg actgggttgt
     8941 tacccatttc cgtcccgtct tccagggcgt gcgcgttccg gttgattata tcctcaacgg
     9001 tttccagcaa ttgctgctgg gtatgccagc gccagtggcg attatcgttt tcgctctcat
     9061 cacctggcag atttccgggg tcggaatggg cgtggcgacg ctggtctcgc tgattgccat
     9121 cggcgcaatc ggtgcctggt cgcaggcaat ggtgactctg gcgctggtgt taactgccct
     9181 gctgttctgt atcgtcatcg gtttgccgtt ggggatatgg ctggcgagaa gtccgcgagc
     9241 ggcgaaaatt attcgtccac tgcttgatgc catgcagacc acgccagcgt ttgtttatct
     9301 ggtgccaatc gtcatgctat ttggtatcgg taacgtgccg ggcgtggtgg tgacgatcat
     9361 ctttgcgctg ccgccgatta tccgtctgac cattctgggg attaaccagg ttccggcgga
     9421 tctgattgaa gcctcgcgct cattcggtgc cagcccgcgc cagatgctgt tcaaagttca
     9481 gttaccgctg gcgatgccga ccattatggc gggcgttaac cagacgctga tgctggccct
     9541 ttctatggtg gtcattgcct cgatgattgc cgtcggcggg ctgggtcaga tggtacttcg
     9601 cggtatcggt cgtctggata tggggcttgc caccgttggc ggcgtcggga ttgtgatcct
     9661 cgccattatc ctcgatcgcc tgacgcaggc cgttgggcgc gactcacgca gtcgcggcaa
     9721 ccgtcgctgg tacaccactg gccctgttgg tctgctgacc cgcccattca ttaagtaact
     9781 ctgcacttgc ccggtgacgc cgggcattat caccctgcca aaaaaaggaa taacaatgcg
     9841 acatagcgta ctttttgcga cagcgtttgc cacgcttatc tctacacaaa cttttgctgc
     9901 cgatctgccg ggcaaaggca ttactgttaa tccagttcag agcaccatca ctgaagaaac
     9961 cttccagacg ctgctggtca gtcgtgcgct ggagaaatta ggttataccg tcaacaaacc
    10021 cagcgaagtg gattacaacg ttggctacac ctcgcttgct tccggcgatg caaccttcac
    10081 cgccgtgaac tggacgccac tccatgacaa catgtacgaa gctgccggtg gcgataagaa
    10141 attttatcgt gaaggggtat ttgttaacgg cgcagcacag ggttacctga tcgataagaa
    10201 aaccgccgac caatacaaaa tcaccaacat cgcacaactg aaagatccga agatcgccaa
    10261 actgttcgat accaacggcg acggtaaagc ggatttaacc ggttgtaacc ctggctgggg
    10321 ctgcgaaggt gcgatcaacc accagcttgc cgcgtatgaa ctgaccaaca ccgtgacgca
    10381 taatcagggg aactacgcag cgatgatggc cgacaccatc agtcgctaca aagagggcaa
    10441 accggtgttt tattacacct ggacgccgta ctgggtgagt aatgagctga agccagggaa
    10501 agatgtggtc tggttgcagg tgccgttctc cgcactgccg ggcgataaaa atgccgatac
    10561 caaactgccg aatggcgcga attacggctt cccggtcagc accatgcata tcgttgccaa
    10621 caaagcctgg accgagaaaa atccggcggc agccaaactg tttgccatta tgcagttgcc
    10681 agtggccgat attaacgccc agaacgccat tatgcatgac ggcaaagcct cagaaggcga
    10741 tattcagggc cacgttgatg gctggatcaa agcccaccag cagcagttcg atggctgggt
    10801 gaatgaggcg ctggcagcgc agaagtaatt tttattcggg cggataaggc gttttcgcct
    10861 catccgccgc tctgtacaca atgcctgatg cgacgctgac gcgtcctatc atgcctacaa
    10921 accacatcgc acatttcaac aagctatctt tcatcccata ttcatcaaca tccgctatta
    10981 ttgatttcca gcttaatcat cacctgatga acaaaaataa tgactaaacc taatcatgag
    11041 cttagcccgg cgctgatcgt gctgatgtct atcgccaccg gtctggcggt agccagtaac
    11101 tattacgccc agccattgct cgacaccatc gcgcgtaact tttccctttc cgccagttcg
    11161 gcaggcttta ttgttaccgc cgcgcagttg ggctatgccg caggtctact gtttcttgtt
    11221 cccctcggtg atatgtttga acgccgccgc ctgattgtct cgatgacctt actggcagcg
    11281 ggcggtatgt taatcactgc cagcagtcag tcactggcga tgatgatcct cggtacggca
    11341 ttaaccggtt tattctcagt cgtggcacaa attctggttc cgctggcagc gacgctggct
    11401 tcaccggata aacgcggcaa agtggttggc accattatga gcggtctgct gttggggata
    11461 ttgctggcac gaaccgttgc cgggttactg gcaaacctcg gcggctggcg caccgtcttt
    11521 tgggttgctt cggtgttaat ggcactgatg gcgctggcat tatggcgtgg tctgccacaa
    11581 atgaaatcag aaacccacct caactaccca cagttgttgg gttccgtttt cagcatgttc
    11641 atcagcgata agatcctgcg cacccgcgcg ttgctgggct gcatgacctt tgccaacttc
    11701 agcattctct ggacctcaat ggcctttttg cttgccgctc caccttttaa ctacagcgat
    11761 ggtgacattg gtctgtttgg gcttgcggga gctgccggag cgttgggcgc tcgtccggcg
    11821 ggcggttttg ccgacaaggg caaatcgcac cacaactttc ggtctgcttc tgctgttact
    11881 ttcatggctg gcaatctggt ttggtcacac ttccgtactg gcgttgatta tcggcattct
    11941 ggtgctggac ctcaccgtac agggcgtgca tatcactaac cagacggtaa tttatcgaat
    12001 acatcctgat gcgcgtaatc gcctgaccgc aggttacatg accagctact ttattggcgg
    12061 tgccgccggt tcgctaattt cagcctcagc ctggcaacat ggcggttggg ctggcgtttg
    12121 tctggctggc gcgacgattg ccctggttaa cttactggtc tggtggcgag gttttcatcg
    12181 tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt ggctttacac ttataagggt
    12241 gttaagaagc ccatcagtct gataaggtta agatattcat tcagtctatt tataatatta
    12301 acaatcgtta agcgtacact ctatggaaag ccctactcca cagcctgctc ctggttcggc
    12361 gaccttcatg gaaggatgca aagacagttt accgattgtt attagttata ttccggtggc
    12421 ctttgcgttc ggtctgaatg cgacccgtct gggattctct cctctcgaaa gcgttttttt
    12481 ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt accgcgatgc tggcagccgg
    12541 gagtagtttg tgggttgctg cactgagcgt catggcaatg gatgttcgcc atgttttgta
    12601 tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa aaatcgaaaa ccgccctatg
    12661 ggcgtttggc ctgacggatg aggtttttgc cgccgccacc gcaaaactgg tacgcaataa
    12721 tcgccgctgg agcgagaact ggatgatcgg cattgccttc agttcatggt catcgtgggt
    12781 atttggtacg gtaatagggg cattctccgg cagcggcttg ctgcaaggtt atcccgccgt
    12841 tgaagctgca ttaggtttta tgcttccggc actctttatg agtttcctgc tcgcctcttt
    12901 ccagcgcaaa caatctcttt gcgttaccgc agctttagtt ggtgcccttg caggcgtaac
    12961 gctattttct attcccgtcg ccattctggc aggcattgtc tgtggctgcc tcactgcgtt
    13021 aatccaggca ttctggcaag gagcgcccga tgagctatga ggttctgctg cttgggttac
    13081 tggttggcgc ggcgaattat tgcttccgct atttgccgct gcgcctgcgt gtgggtaatg
    13141 cccgcccaac caaacgtggc gcggtaggta ttttgctcga caccattggc atcgcctcga
    13201 tatgcgctct gctggttgtc tctaccgcac cagaagtgat gcacgataca cgccgtttcg
    13261 tgcccacgct ggtcggcttc gcggtactgg gtgccagttt ctataaaaca cgcagcatta
    13321 tcatcccaac actgcttagt gcgctggcct atgggctcgc ctggaaagtg atggcgatta
    13381 tataacccac aagaatcatt tttctaaaac aatacattta ctttatttgt cactgtcgtt
    13441 actatatcgg ctgaaattaa tgaggtcata cccaaatgga tagttcgttt acgcccattg
    13501 aacaaatgct aaaatttcgc gccagccgcc acgaagattt tccttatcag gagatccttc
    13561 tgactcgtct ttgcatgcac atgcaaagca agctgctgga gaaccgcaat aaaatgctga
    13621 aggctcaggg gattaacgag acgttgttta tggcgttgat tacgctggag tctcaggaaa
    13681 accacagtat tcagccttct gaattaagtt gtgctcttgg atcatcccgt accaacgcga
    13741 cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga acgtcgtaaa agcgataacg
    13801 atcgccgctg cctgcatctg caattaacgg aaaaaggtca cgagtttttg cgcgaggttt
    13861 taccaccgca gcataactgc ctgcatcaac tctggtccgc gctcagcaca acagaaaaag
    13921 atcagctcga gcaaatcacc cgcaaattgc tctcccgtct cgaccagatg gaacaagacg
    13981 gtgtggttct cgaagcgatg agctaacgcg tcatctcgct caaaaatcca gatttataaa
    14041 agaaaaaatg accggccagc atcgcaacat gctggccttt ttggcaagca ggtcggctca
    14101 gccgatgagt taagaagatc gtggagaaca atatgagcgc aaatgcggag actcaaaccc
    14161 cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct gctcctcctt ctcaccttgc
    14221 tctttataat tattgccgta gcgataggga tttattggtt tttggtactg cgtcatttcg
    14281 aagaaaccga tgacgcatac gtggcaggga atcagattca aattatgtct caggtgtctg
    14341 gcagcgtgac gaaagtctgg gccgataaca ccgattttgt aaaagaaggc gatgttctgg
    14401 tcactctcga cccgacagat gctcgccagg cgtttgaaaa agccaaaact gcactggctt
    14461 ccagcgttcg ccaaacccac cagctgatga ttaacagcaa acagttgcag gcgaatattg
    14521 aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta caaccgccgt gtgccgctgg
    14581 gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc ccgcgacgcc gtcaccagtg
    14641 cccaggcgca actggacgtc gctattcaac aatacaatgc caatcaggcg atgattctgg
    14701 ggactaaact ggaagatcag cctgccgtgc aacaggctgc caccgaagta cgtaacgcct
    14761 agctggcgct ggagcgtacc cgtattgtca gtccgatgac cggttatgtt tcccgtcgtg
    14821 ccgtgcaacc gggtgcgcaa attagcccaa cgacgccgct gatggcggtc gttccagcca
    14881 ccaatatgtg ggtggatgcc aactttaaag agacgcagat tgccaatatg cgcatcggtc
    14941 agccggtcac catcaccacc gatatttacg gcgatgatgt gaaatacacc ggtaaagtgg
    15001 ttggtctgga tatgggcaca ggtagcgcgt tctcactgct tccggcgcaa aatgcgaccg
    15061 gtaactggat caaagtcgtt cagcgtctac ctgtgcgtat cgaactggac cagaaacagc
    15121 tggagcaata cccgctgcgc attggtttgt ccacgctggt gagcgtcaat accactaacc
    15181 gtgacggtca ggtactggca aataaagtac gttcaacacc ggtcgcggta agtaccgcgc
    15241 gtgaaatcag cctggcacct gtcaataaac tgatcgacga tatcgtaaaa gctaacgctg
    15301 gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc tggaaggcgc gcaactggtc
    15361 attatgacga ttgcgctgtc actggcgaca ttcatgcagg tgctgaactc caccattgct
    15421 aacgtggcga tccccactat cgccgggaat ctgggctcat cgctcagcca aggaacgtgg
    15481 gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc cgcttaccgg ctggctggca
    15541 aagcgcgtcg gggaagtgaa actgttcctt tggtccacca tcgcctttgc tattgcgtct
    15601 tgggcgtgtg gtgtctccag cagcctgaat atgctgatct tcttccgcgt gattcagggg
    15661 attgtcgccg ggccgttgat cccgctttcg caaagtctat tgctgaataa ctacccgcca
    15721 gccaaacgct cgatcgcgct ggcgttgtgg tcgatgacgg tgattgtcgc gccaatttgc
    15781 ggcccgatcc tcggcggtta tatcagcgat aattaccact ggggctggat attcttcatc
    15841 aacgtgccga ttggcgtggc ggtggtgttg atgacactgc aaaccctgcg cggacgtgaa
    15901 acccgcaccg aacggcggcg gattgatgcc gtggggctgg cactgctggt tattggtatc
    15961 ggcagcctgc agattatgct cgaccgcggt aaagagctgg actgtttttc atcacaggaa
    16021 attatcattc ttaccgtggt ggcggtggtg gctatctgct tcctgattgt ctgggagctg
    16081 accgacgata acccgatagt cgatctgtcg ttgtttaagt cgcgcaactt caccatcggc
    16141 tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg ctattgttct gctgccgcag
    16201 ttgttgcagg aggtctacgg ttacacggcg acctgggcag gtttggcctc tgcgccggta
    16261 gggattattc cggtgattct gtcgccgatt atcggccgct tcgcgcataa actggatatg
    16321 cggcggctgg taaccttcag ctttattatg tatgccgtct gcttctactg gcgtgcctat
    16381 acctttgaac caggtatgga tttttgcgcg tcggcctggc cgcagtttat ccaggggttt
    16441 gcggtggtct gcttctttat gccgctgacc accattacgc tgtctggttt gccaccggaa
    16501 cgactggcgg cggcatcgag cctctctaac tttacgcgaa cgctggcggg gtctatcggc
    16561 acgtcgataa ccacgaccat gtggaccaac cgcgagtcga tgcaccatgc gcagttgact
    16621 gagtcggtaa acccgttcaa cccgaatgcc caggcgatgt acagtcaact ggaagggctt
    16681 gggatgacgc aacagcaggc atcaggctgg attgcccagc agatcaccaa tcaggggctg
    16741 attatttccg ccaatgagat cttctggatg tcagccggga tattcctcgt actgctgggg
    16801 ctggtgtggt ttgctaaacc gccatttggc gcaggtggcg gcggaggcgg tgcgcactaa
    16861 gtacaactaa gccagttcat ttgaactggc ttttttcaat taattgtgaa gatagtttac
    16921 tgactagatg tgcagttcct gcaacttctc tttcggcagt gccagttctt cgttgctgtt
    16981 gatgcgtacc tcacgttcca gaatgctacg cgcaatatcc tgcgcttcct gcaacgagtg
    17041 catctggtaa gtgccacact ggtagacgtt cagttccggg atctgattct gatcctgcac
    17101 tttcagcacg tcttccattg ccgctttcca ggcatcagca acacgctgct catctggcgt
    17161 accaatcaga ctcatataaa aaccggtgcg gcagcccatt ggcgagatat cgataatctc
    17221 tacaccatta ccgttaagat ggttacgcat aaaaccagca aacaggtgct ccagggtatg
    17281 gatccctctt tctggcatca cttctttgtt cggcacgcag aagcgcagat cgaacacggt
    17341 gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc cgaactgcag gcgcttccat
    17401 ccgggtatga tcgactgtga agctatctaa caacggcatt tagccacctc cggtaatttt
    17461 tttaaaaatt ttctgaactc tttcttccca ggcgagtctg agtatatgaa agacgcgcat
    17521 ttgttatcat catccctgaa ttcagagatg aaattttggc cactcccgag tggccttttt
    17581 cttttctgtc aggcgtgttt ttccagccac accgcaaacg gttcggtatc agcggtttcc
    17641 atttcctgct gacggcgttc agatgcctcg cgctcggcta caaaatcctc ttcgcgcaga
    17701 atttccagcg gctcttcacg cagcagatta cggtaggctt ctgcaaacgc tttgcctgtt
    17761 ccgccaatac cagtatcaat catagacctt aagatacggg cagagaaagt cagatcggga
    17821 ttatcgaagc aggcaaccag ttcatcacac actttctgat acgcttcgcc gccgttaatg
    17881 ctatccagcg tttgcgcgac gcgtttcaga tcgcggaaca gatctttacc cacctgcagt
    17941 aacgggaact gcgcggtttc gcagccgata cccagcgtca ggcccggttt gcgaccttca
    18001 aggatcaccc ggttccagtt aacgcgcgta caggcaagtt cgctactgct catttccggt
    18061 gcatcagcca gcgcacacca gaccataaac aggtcgagga atcgcacctg ctgttcatct
    18121 acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca cttcaatata ttcaatgccg
    18181 ccacgtaaca gcgcatcaga aggtgactcg ccgctgcggg taacgcgttt tggacgaatc
    18241 ggcgcataca gttcgttttc aatctgcagc acgttgctat tgatttgcag cctcttaccg
    18301 tctttctcaa taccaatctt cgcgtactct tccgatggcg ttttgattgc ctgtttaagg
    18361 cccgctacgt actcgtaaag atcgttgaag gtaataccaa gattgctttg cgatttattg
    18421 gtatagccga gatcgctcaa acgaagagag gtcgcatacg gcaggtaata cataccgcac
    18481 tcggttttct caaacggcag cgacgttggt tttccttgca ggaaagaaga acaaatcgcc
    18541 ggagatgcac caaacagata aggaataacc caaccgaaac gatagtaatt gcggataacg
    18601 cggaaatagc ccgcagaaat tttctctttg gcatcagcgc ccgagatatc accgcacttc
    18661 gcttgccaga atgccattgg caaagagaaa ttgtagtgca cgccggaaat ggtttgcatc
    18721 agcgcgccgt agcgattttt cagcccttca cgatacagcg ttttaaagcg tccggtgtta
    18781 gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg cgatgtagca tggcatactt
    18841 aacggccaca tccgctcatc gcccatattg cgcgccgtat aacgatgcag atcgcgcata
    18901 aaggtcagca tatgttcaat atcaccatcc actggtgtaa tgaattccag caatgcttcc
    18961 gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac ctaatgcttc aggatgacct
    19021 gttgttgcca gtgtgccatc agcattaaca cgcaaagttt cgcgctccag cccacgctgt
    19081 atccccttta acgcctgagg atgtttttcc agccaggcca gcgcctgtga tacgtccggg
    19141 atcaaattga cctcccgcct gtcaaaatcg ttttaattag cataactgta atggtgacca
    19201 tatgtgcagg cctacaatta gtgccaccac atcatgccct gaacggtcgc tgctgcaact
    19261 gcaacatagc gtaacgcttt accaaggcac aaaaaaaaga ttactggccc ccaggagatg
    19321 cgcatccatc ccgctaacag acacagtaaa tcgccaacca cgggcatcca gcttaataat
    19381 agcgtgactg caccatagcg tttcagccag ccggtagctt tctcttgcca gcgcgatgtt
    19441 ttacgcaatg gaaagaaacg cccaaggata acgttagtta accctccaag gctattaccc
    19501 attgttgctg ttaaaactaa aacccaggga tgactgatcc cggaaagcaa cattgccacc
    19561 agcacgactt cggagttgcc gggtaatagt gtagcgctga gaaaactact ggcaaacaac
    19621 gagaaaagcg ataacgcttc actcacagca agcgaacatc cacagcgtcc atgcctgctg
    19681 cacgggccgc ctgaataccg aaatcggcat cttcaaagac cacacactgc gtcggttgca
    19741 cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc gggtttatgg tgtttgacgt
    19801 gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc caggtgcgcc agcaatgcct
    19861 cagcgatggc gctttcactc cccgttccta cagccattgg gcgacgacca tgccaacttt
    19921 tcaccacttc aacaagagga agcggctcga cactatccag cagcatactt cttactgctt
    19981 ctgttttttc acgcgctaac gcatgcgggt cgagatcggc ctgattcagc tcaataattg
    20041 cctgagcaat acgccaggtg ggcgatccat taagcgcaat catcgcctga atatcgtact
    20101 gaagaccgta gtgccctaat acttcgcgcc acgctttacg gtgcgtaggc gccgtatcca
    20161 ggattgtgcc atccatatca aaaattaaac ctgcataacg ctcgtacatg gtcttctcgc
    20221 aagtcgaaaa atatgacgct actttagcgt aattgcttga ttttgtcgct gatggcgaag
    20281 gagaagaaaa ttcaggaaat ggataaagta gttatttaga aatgagatat ttttgaagga
    20341 aattttttgg agaatatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta
    20401 gccgagtact ctatccagct gagctacgga t
//
LOCUS       DAJTYM010000070        20402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTYM010000070 DAJTYM010000000
VERSION     DAJTYM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..4226)
                     /gene="mukB"
                     /locus_tag="OO575_002651"
     CDS             complement(<1..4226)
                     /gene="mukB"
                     /locus_tag="OO575_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6665563.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAA"
     gene            complement(4226..4930)
                     /gene="mukE"
                     /locus_tag="OO575_002652"
     CDS             complement(4226..4930)
                     /gene="mukE"
                     /locus_tag="OO575_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6665564.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4911..6233)
                     /gene="mukF"
                     /locus_tag="OO575_002653"
     CDS             complement(4911..6233)
                     /gene="mukF"
                     /locus_tag="OO575_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6665565.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6230..7015)
                     /gene="cmoM"
                     /locus_tag="OO575_002654"
     CDS             complement(6230..7015)
                     /gene="cmoM"
                     /locus_tag="OO575_002654"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6665566.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7151..7930
                     /gene="elyC"
                     /locus_tag="OO575_002655"
     CDS             7151..7930
                     /gene="elyC"
                     /locus_tag="OO575_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6665567.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7907..8800)
                     /locus_tag="OO575_002656"
     CDS             complement(7907..8800)
                     /locus_tag="OO575_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6665568.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8954..9700)
                     /gene="kdsB"
                     /locus_tag="OO575_002657"
     CDS             complement(8954..9700)
                     /gene="kdsB"
                     /locus_tag="OO575_002657"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6665569.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9697..9879)
                     /gene="ycaR"
                     /locus_tag="OO575_002658"
     CDS             complement(9697..9879)
                     /gene="ycaR"
                     /locus_tag="OO575_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6665570.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9931..11163)
                     /locus_tag="OO575_002659"
     CDS             complement(9931..11163)
                     /locus_tag="OO575_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6665571.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11200..12186)
                     /gene="lpxK"
                     /locus_tag="OO575_002660"
     CDS             complement(11200..12186)
                     /gene="lpxK"
                     /locus_tag="OO575_002660"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6665572.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12183..13931)
                     /gene="msbA"
                     /locus_tag="OO575_002661"
     CDS             complement(12183..13931)
                     /gene="msbA"
                     /locus_tag="OO575_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6665573.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(13968..16227)
                     /locus_tag="OO575_002662"
                     /pseudo
     CDS             complement(13968..16227)
                     /locus_tag="OO575_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16434..16718)
                     /gene="ihfB"
                     /locus_tag="OO575_002663"
     CDS             complement(16434..16718)
                     /gene="ihfB"
                     /locus_tag="OO575_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6665574.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16878..18551)
                     /gene="rpsA"
                     /locus_tag="OO575_002664"
     CDS             complement(16878..18551)
                     /gene="rpsA"
                     /locus_tag="OO575_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6665575.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18662..19345)
                     /gene="cmk"
                     /locus_tag="OO575_002665"
     CDS             complement(18662..19345)
                     /gene="cmk"
                     /locus_tag="OO575_002665"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6665576.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19518..20282)
                     /gene="ycaL"
                     /locus_tag="OO575_002666"
     CDS             complement(19518..20282)
                     /gene="ycaL"
                     /locus_tag="OO575_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6665577.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4909 a         5656 c         4948 g         4889 t
ORIGIN      
        1 gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg caggcggcga
       61 gattcatctt cccagctttg taccaccatc accagaatcg acataccggt accaatcgcc
      121 tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc acggttaacc
      181 tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat ggtctgcggc
      241 gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc caacgcttcc
      301 gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg ttcagagagc
      361 acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac gctgttcacc
      421 tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt ctgctcgcgc
      481 tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag tttctgttca
      541 cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc gatagcttcc
      601 accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg ctgataaacc
      661 gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat gcgcagcacg
      721 tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc cttatccgac
      781 atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa gcggcgctca
      841 acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc ggtcactacc
      901 tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt caggttgtcc
      961 atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga acggttattg
     1021 ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc cccgctatca
     1081 gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc ttttttggtg
     1141 tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg cgctgcgtga
     1201 ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag acgttcacgc
     1261 agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga atagctaaag
     1321 tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc atcgcgctgc
     1381 atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg gtcgctctgc
     1441 aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg aacaaaacgc
     1501 gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc cgccaggctg
     1561 tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac gccttctttc
     1621 gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact caacgcccgc
     1681 tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc agactcaaac
     1741 gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg ctgggttttc
     1801 tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc ggcatggagg
     1861 ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg gaaacgtgaa
     1921 taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc atcaacgctg
     1981 aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc ttccgggcaa
     2041 tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac gatggcgtgg
     2101 cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac gtcgtcataa
     2161 atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg ctgatcttca
     2221 gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt gcgcgcgcct
     2281 acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg cagatattca
     2341 gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg gttgagactg
     2401 ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg agactgcagc
     2461 tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac gctatcggaa
     2521 agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc aatatcaaaa
     2581 ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg ctgctcacgc
     2641 agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc tgccaggtga
     2701 cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc gttacgcgcc
     2761 agcgggccgt tgattgccac caccagctga taagcctgct caaactggct gtgcgcggtt
     2821 tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc cagctctttc
     2881 gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg cagatggcac
     2941 agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc gcgcgtttgc
     3001 tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc cacttccagt
     3061 tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc tgccaccact
     3121 tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta gcgttcgatc
     3181 ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc cgcctgataa
     3241 tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc catatcgacg
     3301 tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg acgaaactcc
     3361 agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc cgccgccacg
     3421 tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg ggtgacacga
     3481 atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg gaacgcttta
     3541 cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat ggcactggag
     3601 atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc agatgcggag
     3661 cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc ggtaatggag
     3721 ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag ctcgttaagc
     3781 ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt cggctgcacc
     3841 gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc gcgtccggca
     3901 acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat ggtgtcgagc
     3961 atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg cgaaccgctg
     4021 gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg gatcagcgcc
     4081 gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga aagcgtcgtg
     4141 accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat cagcgtcagt
     4201 gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc tgactctctt
     4261 cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca tcacgaatca
     4321 ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg aagcggaaca
     4381 ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc atgcctaaac
     4441 gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg tcaacgtctg
     4501 aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc agggtgagca
     4561 gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt tccgggctga
     4621 gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag acggaacgcg
     4681 ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg cgaataagct
     4741 caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc aggaatgcat
     4801 gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg tccagcgccg
     4861 gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt tcaatatttg
     4921 tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca tttcgctggc
     4981 agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat aacaatacgc
     5041 gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac caccagacca
     5101 agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat gatcgccgcc
     5161 agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag ttccccagtc
     5221 acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg atcggcattg
     5281 gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg taaccgctga
     5341 gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg tacgtggcgg
     5401 tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg atcgagtttg
     5461 ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt catcgtcgca
     5521 tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag cgtatcctgc
     5581 aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga aatcgccgcc
     5641 cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg ctgttcgtcc
     5701 atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata tttcagtggc
     5761 gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc atctgctgcg
     5821 cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt agaaaactcg
     5881 cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg gtaaattgcg
     5941 ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac catgtcgttg
     6001 atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc atcactcaca
     6061 tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg ctcgccgttc
     6121 agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat ggagaagtca
     6181 ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact catactttat
     6241 ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta atatacggtt
     6301 cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg cgctgctggt
     6361 acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata atttgccaac
     6421 cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct ggcgaaagcg
     6481 tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg gcgaccatgt
     6541 tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg cctggacgca
     6601 acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg agcaccgcat
     6661 ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga gcggcgcaat
     6721 gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt gcgcggtcga
     6781 tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc attttgattg
     6841 cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg cccatttccg
     6901 ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct ttggtggtgc
     6961 cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc tgcattgctt
     7021 tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac agccacggcg
     7081 agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt taaaaaagga
     7141 accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc tgccacttcc
     7201 gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc gttttcaaaa
     7261 aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga gtttgcaacc
     7321 ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga ataattcgca
     7381 aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc agtgggcacc
     7441 gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc gtttatggcg
     7501 cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca atacggtgag
     7561 tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg agcaaattat
     7621 caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac aggcgattgg
     7681 tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga tgatcttttt
     7741 tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca tcgactcgcc
     7801 gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata gcgatcgtgt
     7861 tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag gcgagccacg
     7921 gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg gtattaacca
     7981 gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac gactccgcca
     8041 ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta tccattagtc
     8101 ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc atcgccagta
     8161 actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg tgaatcaaca
     8221 cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg cgaaacagga
     8281 tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg gtccatagca
     8341 cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg tcgaccgcgc
     8401 cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg atttggtctt
     8461 tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca ccacgcatcc
     8521 gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca ccataaatag
     8581 tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc agttgtcggg
     8641 cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc tggctgtcat
     8701 acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga ctgagttttt
     8761 cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc ctcataaaag
     8821 ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga tcaattgttg
     8881 aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt ggctgaagtg
     8941 tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca ggggtatcca
     9001 cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg taccacagaa
     9061 cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag ttgacgtaac
     9121 gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag ttatcgccaa
     9181 cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca cgtgagaagt
     9241 acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta aacgcttctt
     9301 ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga gcgaggttat
     9361 cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc acattaacga
     9421 tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt tctgttcctg
     9481 actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg gcgcgggcaa
     9541 catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc gcgcgttcaa
     9601 gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg ggcagacgcg
     9661 tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct catcagcagt
     9721 cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg ggaaagccag
     9781 gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt ttccgttgca
     9841 aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag atggatcggg
     9901 ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt cccagccagt
     9961 gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg ttgcctgctg
    10021 ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg tcgttggttt
    10081 aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc gatgcatttt
    10141 ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa aatagccata
    10201 ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa aaagctgctc
    10261 tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc tgtgcgttgc
    10321 agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat gcagccaaag
    10381 attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc tcgcttcgcg
    10441 ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact gttcgcggaa
    10501 tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg tttgcgaaac
    10561 gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat aaacgcgctg
    10621 gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt ccagatgccg
    10681 tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct caaaatcggc
    10741 tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt ccgcctcatg
    10801 ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca gcatgcggtg
    10861 gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc agtattccat
    10921 taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc ccagacgact
    10981 gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt gcagcaagga
    11041 catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg gtttgtttaa
    11101 caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt gcggcagcga
    11161 cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag aagccagcga
    11221 ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt ctacaggcaa
    11281 ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt cagtcattac
    11341 cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag actgatgatc
    11401 ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg caaaaaagcg
    11461 cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa cgtcacaacg
    11521 cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga tttcaccgct
    11581 gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc ccgctcgctc
    11641 acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac catcaataac
    11701 gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg ttacgatgat
    11761 ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa cgggagaaac
    11821 cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg cctgtgctgt
    11881 ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat atccccgcga
    11941 tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga caacgaccgg
    12001 ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta cgggggcacg
    12061 ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca ccaggccata
    12121 caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc agattttttc
    12181 gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg ctcaagcaaa
    12241 tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat ttcgtctgcc
    12301 ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt ctgcaactca
    12361 tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt agcttcatcc
    12421 agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg ctgacgctga
    12481 ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt atccatctta
    12541 ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc acggctgtac
    12601 tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag atggacattc
    12661 tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg cagatcgtga
    12721 ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag gctggcgatg
    12781 gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc cggaattttc
    12841 aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt gacattgcgg
    12901 aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt ctcctgctca
    12961 ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa ctgggcgtta
    13021 acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa aacaacggta
    13081 atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata cagaacaaac
    13141 gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga ggctgaaacc
    13201 attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt cgtttccact
    13261 tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc tgcactggtg
    13321 gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg cttcgatact
    13381 acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga cagttgccaa
    13441 ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg cacaacagta
    13501 atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat acgtgacaac
    13561 agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat gtgaccaaac
    13621 aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca gtagctggag
    13681 acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg catccacacc
    13741 agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag gagcgataac
    13801 atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat cagacccgct
    13861 ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga gagatctttg
    13921 tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta cccgttatcc
    13981 actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct gaggctgcta
    14041 atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg agtatcaagc
    14101 cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa tcgccatgcg
    14161 ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat taatggcaac
    14221 gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg cacttgctgc
    14281 cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt tagaagaatg
    14341 ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt atcgttgcta
    14401 ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc ttcgccacgc
    14461 acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat cggccatata
    14521 tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag aataacgcct
    14581 tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc actatcccct
    14641 tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca ccattgccag
    14701 cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt tttgccacag
    14761 cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc gccaggcgtt
    14821 taatcgccat accaccatta agaaccatgg ggaaaatgat agccattgcc aacattcaac
    14881 aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag ccaaagaccg
    14941 gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat gcacaaccat
    15001 cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa atagatttgc
    15061 aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca taagcaggag
    15121 tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg gcggcagttg
    15181 ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg cagcggcaga
    15241 gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg ccgccagaca
    15301 acatatccat acatcccagc cactccactg tcgcccactt aacttaagca ttccccacgt
    15361 agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa
    15421 agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc gcccaggcag
    15481 aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa
    15541 tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt tgatttcttt
    15601 ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc acggataggg
    15661 ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat tcggttcaat
    15721 gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg cataacgctg
    15781 gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga gtttcattga
    15841 ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca caaggcctgg
    15901 cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag tggtcatgcc
    15961 gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc
    16021 ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat
    16081 ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag tcagaaacgc
    16141 aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc cgcaaattat
    16201 gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa gtgtgcaact
    16261 ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca gaagaattta
    16321 acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct gattagagaa
    16381 ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg
    16441 taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac gtattttcct
    16501 tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc
    16561 aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag agtcgaggcc
    16621 atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat gtgcgattgc
    16681 tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt tccttaaagg
    16741 caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc ccttagtgct
    16801 tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg gaataaaaat
    16861 cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc cattgcgttg
    16921 ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct
    16981 tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac gccggtgaat
    17041 ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac gcggtcacgg
    17101 gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac ggttgcgcct
    17161 ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt cagagcaacc
    17221 cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg ttcacgttct
    17281 gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct
    17341 tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg
    17401 tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc aacacggtcg
    17461 cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg tttcagaccc
    17521 agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac tacatcgcca
    17581 acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt
    17641 accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt caggttggtc
    17701 acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac ccacggatct
    17761 tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa cttcagcact
    17821 ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac gcgtttccag
    17881 gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa tgcaccgtag
    17941 tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt ttccagcagc
    18001 tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac aacaacgttg
    18061 ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc caggtgcaga
    18121 gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg aataccgttc
    18181 agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac agtttcagca
    18241 tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt ctcacgggac
    18301 agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac ttcgtcacct
    18361 acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat ggcggactca
    18421 gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac aacgccacga
    18481 acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag ttgagcaaaa
    18541 gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc catgccggat
    18601 ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca aattcggtcg
    18661 cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc aattgctcaa
    18721 tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt ggcgctaccg
    18781 ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc tcgaagttaa
    18841 cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct tccgaggagg
    18901 cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg tcgcggccat
    18961 cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa
    19021 cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta cgaatttcgc
    19081 cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac acaaaacgta
    19141 catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca acatggtgat
    19201 gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga tgccattgca
    19261 acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg ccatcaatgg
    19321 taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac cgttaacata
    19381 accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt
    19441 ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag aagaccagcc
    19501 cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg ttgcgcacgc
    19561 tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc caggctagcc
    19621 agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt caacagatca
    19681 aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt aatagcgcct
    19741 tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct ggtagctgaa
    19801 atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag cgagtgaccc
    19861 aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc attcatcatg
    19921 tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc gttgacgtcg
    19981 ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc caatgctttg
    20041 gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg attgccgctg
    20101 tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct taatgttgct
    20161 gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc gtgggtattt
    20221 tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt agtattcttc
    20281 atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac ttacattgac
    20341 aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt
    20401 gc
//
LOCUS       DAJTYM010000071        20392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTYM010000071 DAJTYM010000000
VERSION     DAJTYM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..132
                     /locus_tag="OO575_002667"
     CDS             <1..132
                     /locus_tag="OO575_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6665578.1"
                     /translation="FTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(192..1490)
                     /gene="eno"
                     /locus_tag="OO575_002668"
     CDS             complement(192..1490)
                     /gene="eno"
                     /locus_tag="OO575_002668"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6665579.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1578..3215)
                     /gene="pyrG"
                     /locus_tag="OO575_002669"
     CDS             complement(1578..3215)
                     /gene="pyrG"
                     /locus_tag="OO575_002669"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6665580.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3443..4234)
                     /gene="mazG"
                     /locus_tag="OO575_002670"
     CDS             complement(3443..4234)
                     /gene="mazG"
                     /locus_tag="OO575_002670"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6665581.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4330..6564)
                     /locus_tag="OO575_002671"
     CDS             complement(4330..6564)
                     /locus_tag="OO575_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6665582.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(6612..7913)
                     /gene="rlmD"
                     /locus_tag="OO575_002672"
     CDS             complement(6612..7913)
                     /gene="rlmD"
                     /locus_tag="OO575_002672"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6665583.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            7970..10726
                     /gene="barA"
                     /locus_tag="OO575_002673"
     CDS             7970..10726
                     /gene="barA"
                     /locus_tag="OO575_002673"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6665584.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(10958..12298)
                     /gene="gudD"
                     /locus_tag="OO575_002674"
     CDS             complement(10958..12298)
                     /gene="gudD"
                     /locus_tag="OO575_002674"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6665585.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12319..13659)
                     /locus_tag="OO575_002675"
     CDS             complement(12319..13659)
                     /locus_tag="OO575_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6665586.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(13661..15013)
                     /gene="gudP"
                     /locus_tag="OO575_002676"
     CDS             complement(13661..15013)
                     /gene="gudP"
                     /locus_tag="OO575_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6665587.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15447..15896)
                     /locus_tag="OO575_002677"
     CDS             complement(15447..15896)
                     /locus_tag="OO575_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6665588.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(15914..16696)
                     /gene="truC"
                     /locus_tag="OO575_002678"
     CDS             complement(15914..16696)
                     /gene="truC"
                     /locus_tag="OO575_002678"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6665589.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(16696..17025)
                     /locus_tag="OO575_002679"
     CDS             complement(16696..17025)
                     /locus_tag="OO575_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6665590.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(17647..18192)
                     /gene="syd"
                     /locus_tag="OO575_002680"
     CDS             complement(17647..18192)
                     /gene="syd"
                     /locus_tag="OO575_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6665591.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18260..19108
                     /gene="queF"
                     /locus_tag="OO575_002681"
     CDS             18260..19108
                     /gene="queF"
                     /locus_tag="OO575_002681"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6665592.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19220..>20392
                     /gene="ppnN"
                     /locus_tag="OO575_002682"
                     /gene_synonym="ygdH"
     CDS             19220..>20392
                     /gene="ppnN"
                     /locus_tag="OO575_002682"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6665593.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRA"
BASE COUNT         4982 a         5486 c         4898 g         5026 t
ORIGIN      
        1 tttactttca ccatatttat ggtctttacc tgtttatgtg tacttcagaa aagagcatct
       61 ggtagaagtt atcattcaga caacagcggt tccgctggag gctcaagtgg cggcgcatcc
      121 ggccgctggt aactccacca taaaaaatgc cagcccgaag gctggcatta tcaaatcaga
      181 taaagtcaga cttatgcctg gcctttgatc tctttacgac cgttgtacgg tgctttttcg
      241 cccagagctt cttcgatacg aatcagctgg ttgtatttag caacacggtc agaacggctc
      301 atagaaccag ttttgatctg gcctgcagca gtaccaacag ccagatcagc gatggtagca
      361 tcttcagttt caccagaacg gtgagagata actgcagtgt agccagcatc tttcgccatc
      421 ttgattgcag ccagagtttc ggtcagagaa ccgatctggt tgaatttgat caggatggag
      481 ttagcgatac ctttttcgat accttctttc aggatcttgg tgttggttac gaacaggtcg
      541 tcaccaacca gctggatttt gtcgcccaga actttggtct ggtatgcgaa accgtcccag
      601 tcagattcgt ccagaccgtc ttcgatagaa acgatcgggt actgtttggt cagttcttcc
      661 aggaagtgag tgaattcttc agaggtgaac gctttgttgc cttcgccagc cagaacgtat
      721 ttaccatctt tgtagaattc agaagctgcg cagtccatcg ccagagtgat gtctttgccc
      781 agttcataac cagcagcttt aacagcttca gcgataacag ccagagcttc agcgttggaa
      841 cccaggttcg gcgcatagcc accttcgtca ccaacagcag tgttcatgcc tttcgctttc
      901 agaacttttg ccaggtgatg gaaaacttca gaacccatgc ggatggcttc tttcacagtt
      961 ttcgcgccaa ccggctgaat catgaattcc tggatatcaa cgttgttgtc agcgtgctca
     1021 ccaccgttga tgatgttcat catcggaacc ggcatggagt atttgcccgg agtgccgttc
     1081 agttcagcga tgtgctcgta cagcggcata cctttagcag ctgcagcagc tttggcgtta
     1141 gccagagata cagccaggat tgcgttcgcg ccgaatttgg atttgttttc ggtgccgtcc
     1201 aggtcgatca tgatcttgtc aatgccagcc tgatctttag catctttgcc aatcagcgcc
     1261 tgagcgatcg ggccgtttac cgcagcaaca gctttggtta cgcctttacc caggaaacgg
     1321 gatttgtcgc catcgcgcag ttccagagct tcacgggaac cagtagaagc acctgacgga
     1381 gcagctgcca taccgacgaa accaccctcc agatgtactt cggcttcaac agtcgggtta
     1441 ccacgggagt cgatgatttc acgaccgatg atttttacga ttttggacat taggttttcc
     1501 tcaagtcact agttaaactg aaactccaga caaacaacgc gtacccaagg tacgcgttgc
     1561 cgttctaact tttttactta cttcgcctga cgtttctgga actcgctggc ggctttcaca
     1621 aagcctgcaa acagcgggtg accatcacgt ggagtagaag taaactccgg atggaactgg
     1681 caagccacga accacgggtg attcggaact tcgatgatct cgaccaactg atcatccccg
     1741 gaacggcccg caacgcgcag acctgcatct tcaatctgtt tcaacagcat gttgttgact
     1801 tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat tgtatagctg gcgaaccagg
     1861 ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc gagatcgctc
     1921 ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc actcggtaat cagcgccaca
     1981 accgggtact tacagtctgg cacaaattct gtagagttgg cgttctccat gttggcaaca
     2041 tggcgagcgt aatcaattaa cgccacctgc atacccaggc aaatgcccag ataaggaata
     2101 ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg atagccgaaa
     2161 ccgccaggta cgaggattgc gtccagacct ttaaggattt caacgccgcg cgtttcaaca
     2221 tcttgtgaat cgatcagttt gatgttgacg ctgacacgat tcttcagccc accgtgtttc
     2281 agtgcttcga tcactgattt ataagcatcc ggcagttcaa tgtacttgcc gaccataccg
     2341 atggtgactt cacttaccgg attcgcttct tcgaagataa cctgttccca ttcggacaga
     2401 tttgcttccg ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag cccctgagat
     2461 ttcaacaggc ccggaatttt atagatggaa tcgacgtctt tcagagaaat aaccgctttt
     2521 tccggaacat tacagaacaa tgcaatcttc gcacgttcgt tcgccggtac agcgcgatct
     2581 gaacgacaaa tcaggatgtc aggctggata ccgatggaga gcagctcttt tacagagtgc
     2641 tgagtcggtt tggttttgac ttcaccagac gctgccatgt acggcaccag cgtcaggtgc
     2701 ataaacagag tgtgctcacg gccaatttca acagccatct ggcgaatagc ttcgaggaac
     2761 ggcaaggatt cgatatcacc tactgtaccg ccgatttcta ccagtactac gtcatgacct
     2821 tcgccacctt ccagcacgcg ctctttgatt gcgttagtga tgtgcggaat aacctgcacg
     2881 gttgcgccga ggtagtcacc gcggcgttct ttacgcagaa cgtcagagta gatacgaccc
     2941 gtggtgaagt tgttgcggcg gctcattttg gtacgaatga aacgctcgta gtgccccagg
     3001 tccaagtcgg tttcagcgcc gtcttcagta acgaacactt ccccgtgttg gattgggctc
     3061 atagtacctg gatcgacgtt gatgtaagga tccagtttca tgatggtcac attgaggcca
     3121 cgggcttcaa gaatggctgc gagggaggct gcggcaatgc ctttacccag agaggatacg
     3181 accccgccgg tcacaaaaat atagttcgtt gtcatgctga acctgagaag ttaggttgaa
     3241 aagacgatgg aataaccaag acgggaaagc agtatacccg aacatgacct gtgccacaaa
     3301 ctttcattat ccctcctctt cgccagcgca ctattgaaat caggagtgag aaaatagccc
     3361 ctttggggta aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa tgacacaaat
     3421 ggcgcttgac cgcgtaattc ccttagagat caatttcctg ccgttttacc tgttgccaga
     3481 cttcttccat tgtttcgagg tcaacacctg tcatttccag tccacgcgcg gcaacaatac
     3541 gctccacttc gcgaaaacga cgctcgaatt tttcgttcac tttttgcaat gcgatttctg
     3601 ctttcgtccc taaatggcga gccagattaa ccgtggcaaa cagcaggtcc cccatttcct
     3661 cctccagttt agcctggtcg acaacagcct gccgcgcctc gtacatcacc tcgtcgatct
     3721 cttcgtagac tttatcaacc accgggccaa gcgtcgtcca gtcgaagcca acgttggcgc
     3781 aacgtttctg gattttttgc gcccgcatta aagccggtaa actacgagga atatcgtcca
     3841 gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc caacgggcaa
     3901 gcacttcact actgttttcg gcagaactat cagcaaaaac atgcggatgg cgacgctcta
     3961 atttatcgct aatagcagcg caaatatcat taaagtcaaa gcgcccttct tcctgagcca
     4021 tttgcgcgta aaacaccacc tggaatagca gatcgcccag ttcaccgcga agatcgtcaa
     4081 aatcttcacg ggcgatggcg tccagcactt cgtaggtttc ttcaagggtg taaggcgcaa
     4141 tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt ttccggatcg cgcaggcgct
     4201 gcataatagt gagcaaacgg tcgatttgat tcattgaatt gtcctgaaaa ttgcgggtct
     4261 gtcaggtgta ataccttcga gtaaaagata tcgctggata cggctgacgc catatccagc
     4321 tatgtaagac taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4381 tttacccagc acgcgcccca gcacttgcag gttgtaaatc tcaatggtca tgtcgatggt
     4441 cgccagttgc tgtttggtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4501 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     4561 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     4621 cgcatgggag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     4681 tccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     4741 gtggtgcatc aggttaccaa caccttcaac caccacacga ccgttatctt tactgcggtt
     4801 ttgcggtgtg tagctttttt gctgaagctg tttcagcgcg gcggcgtcct gctcttcggc
     4861 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     4921 gccaccaatc gccgccagca actcgtcgac atcattgaag ttgtaacgcg gcagcagatg
     4981 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5041 cccagccaga atgtttttgt cacggtcctg tttacggaac caggcgtgaa ttttcgaacg
     5101 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5161 ctgtttctgg gtgataattt caatctggtc gcccatttgc agctggtagg tgaacggcac
     5221 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5281 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5341 gacatacacc cggtcgtcaa acacctgact acgtacttcg tcgagcattt cgccggaatc
     5401 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5461 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     5521 ctccgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt ttccacccgg
     5581 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacgtaatc
     5641 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcataaca
     5701 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcgaaggc
     5761 gaggttcttt ttctgcattt tgcgccagat gctgtagatg tgtttcggac gaccatacac
     5821 ttcagcttta acgccttcag ctttcatctc agcgcgcaga tggccgacaa actcttcgat
     5881 atagtgttcg cggtcgagac ggcgttcatg cagcagtttt gcgatgcgtt tgtattcggt
     5941 cgggtggaga taacggaagc agtaatcttc cagctcccat ttcagttgcc caatcccaag
     6001 acggttcgcc aacggcgcgt agatattggt gcactctttt gccgccagta cacgttcatc
     6061 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6121 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6181 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6241 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6301 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6361 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6421 cccctgcgtc tgttgcagac aatacgccca ggtttcggct aagcactcac acgacttctg
     6481 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     6541 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     6601 gcaaatcgta actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     6661 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     6721 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     6781 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     6841 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     6901 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgca
     6961 ggaacacctt ctacaccgac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7021 cccataccgc agaacagatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7081 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7141 aaagttaagc gcaacccgtt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7201 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7261 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7321 gttgcctgta ccagttcaac atgaccaaga tggcgcatag cttgtaagct gcccagacat
     7381 gccctgactt tgggcagcaa tgcttcaagt tggggcgcta aaatggggca ttgtttgacg
     7441 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7501 aagtagttca gacttaaacg cgcgcggcgg cgatagcccc agggaacatc ggcgatcact
     7561 tcagagacat cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     7621 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     7681 gtttcgcgtt ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     7741 ttatcttcag taacagcaac ttccgcgttt tcctgcggca ataatccggg gataaataac
     7801 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     7861 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     7921 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     7981 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8041 gctgagtatc tttttcgtcg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8101 cggtgctagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8161 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8221 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8281 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8341 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8401 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgcttgagct
     8461 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcct gcgtgatgat
     8521 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     8581 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     8641 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     8701 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     8761 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttggatc tggcgaaaaa
     8821 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     8881 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     8941 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9001 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9061 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9121 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9181 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9241 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9301 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9361 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccgggct
     9421 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9481 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
     9541 tattatcgaa gggccatcca tccagtgcct cgcaggtaag cgcctggcct atgtcgaacc
     9601 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
     9661 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
     9721 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
     9781 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagttaatg ctgaaaaact
     9841 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
     9901 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
     9961 taagctggca atgacagtca tggcggttga tgacaacccc gctaacctga aacttatcgg
    10021 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcagt
    10081 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgccggacat
    10141 ggatggcatt cgggcctgcg aactcatcca ccaactcccg catcagcgac aaacgccggt
    10201 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10261 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10321 caagcctggc agcggtattt cgtctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10381 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10441 tttagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10501 tgaggaacag ctggctggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    10561 tggcagttgc ggctatagcg gcgtaccacg tatgaagaat ctctgccagc tgatcgaaca
    10621 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    10681 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    10741 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    10801 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    10861 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    10921 aaccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    10981 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11041 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11101 cacccagccc cggtttttct ggcacctgta ccagcccgcc tttgatctca aacggttctt
    11161 tggtcaggcg ctgattacct tcctgccaga tccagtgcgt atcaatagca gtaattttac
    11221 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11281 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11341 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11401 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11461 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca cacggatctt
    11521 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    11581 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    11641 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    11701 catatttttc atacgccgct tccgccaggc gcaccaccgc atccggcgtc atcgcttctt
    11761 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    11821 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    11881 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    11941 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12001 cgcgcccact agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12061 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12121 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12181 tattacgcgt gaagaacggt gcgtgtgcac cactcagatt catcagcata ctgtcatgac
    12241 ccgccaccgg gatgacctgc atttcagtaa caacaggcgt cgtaaattga gaactcatat
    12301 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12361 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgcaag gcagacgttt
    12421 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12481 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    12541 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    12601 cacatgggta aatatcgcca gagagatatc gaaatggttg ttggaatggc agccccaggt
    12661 cagcccccag tcgtcgcaaa gctgcgccac gcggactgca ccggaaagcg tccagaagtg
    12721 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    12781 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgtcgaa actccgccat
    12841 cacttcacgt ccggagaagc cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    12901 atcattcagc cctttgcata aagaaatggc ttcatcaagc agccatgcac cgttgggatc
    12961 aacggtaatc cgcgcatccg ggaagctttt cttcaatgca cgaacagtgt cgatttcttg
    13021 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13081 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13141 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13201 tatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13261 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13321 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccaaagg tatcaaaatc
    13381 ggctgcctga ttacctttat gtacctgctg aaccacttta ttcaggcgcg caacttcctg
    13441 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13501 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acgatattgc gggtgaagta
    13561 tgcgttatgc gcaccaccaa tattgagcaa catgctgtca tgtccggcga ccggaatgac
    13621 tttcatatca gtaataacag gactggattg tgtcgccatc ttattgcccc gcgacaggtt
    13681 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaggg
    13741 catgaacacc aacataaatc agcgccccat taaacgagcc agtcgtgcca acgatataac
    13801 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    13861 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    13921 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    13981 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14041 caatgttcag cgatcccgtg cggcgcatca gccagtcgga aataattcca cccagcacac
    14101 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14161 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14221 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agctgtttga
    14281 tctgccccca cttcacgctg aacggcactt taactttggt gctttgctga tccatattga
    14341 tcagcgcgcc acccgcggcg atatactcca gctctttctg atttaccccc ggatgttgat
    14401 ttggctcgtg gatgactttc aaccagataa agctgatgac aatgcccaga ccgcccataa
    14461 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    14521 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgca
    14581 ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct tcagcaagcc
    14641 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    14701 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    14761 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    14821 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    14881 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    14941 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15001 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15061 gacatcgcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15121 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15181 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15241 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggtaaaaacg
    15301 aagaaataaa gaagagtatg aaatggatcg cttgactcta ggcaaacgcc agtaaaaatc
    15361 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15421 aacgacattt accgctcgct gacatatcag gacaacagcg tgccccactg ttcgacccac
    15481 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    15541 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcaaaaa
    15601 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    15661 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    15721 gtcgtggacg taaccaccag aacatactta tcctgatagg gcagccagtc gcttaattca
    15781 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    15841 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    15901 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    15961 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16021 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16081 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16141 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16201 gtcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16261 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16321 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16381 ccaccagtgg ataatccaac accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16441 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16501 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    16561 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    16621 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    16681 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    16741 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    16801 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    16861 cagcccccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    16921 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    16981 ttcaagcgcc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacctcaata
    17041 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17101 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17161 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17221 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17281 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17341 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17401 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17461 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    17521 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    17581 cttatttcct ggcgatctcc tacagagtct gtaagagatc tctcacaacc agatggggta
    17641 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatt
    17701 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    17761 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    17821 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    17881 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    17941 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18001 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18061 agttgaaata atgcacggtg aaggaacacc gtacaattcc tcacttaacg gccagctttt
    18121 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18181 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18241 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18301 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    18361 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18421 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    18481 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    18541 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    18601 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    18661 tgatgaactg gaaggccagc cgataggtca tttcaatggc acttgcattg atgaccagga
    18721 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca ccagcggtga
    18781 aaaagtagtg gaagagacgc tcgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    18841 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    18901 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    18961 acgcatcttt aatgacctgt tacgcttctg ccagccagag aaattgagcg tttacgcacg
    19021 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19081 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19141 cagaaggttg tgaaaggtca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19201 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19261 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19321 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19381 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19441 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgctttgca
    19501 ggccaacttg ttcgcggttc tgcgagacat tctcttcgtt tacgggcaaa tccatcatac
    19561 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    19621 catcttgcgt aacgctcgcg cgctgcatgt gggtgaagcg ccaaatatgg tggtctgctg
    19681 gggcggtcac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaaccagct
    19741 gggcctgcgt gagctgaata tctgcaccgg ctgtggtcca ggagcgatgg aagcgccgat
    19801 gaaaggtgct gcggtcggac acgcgcagca gcgttacaaa gacagtcgtt ttattggtat
    19861 gacagagccg tcgattatcg ccgctgaacc gcctaaccca ctggtcaacg aattgatcat
    19921 catgccggat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    19981 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20041 cccggccaac aaagatcagg ttttaccatt gatcctcacc ggcccgaaag agagcgccga
    20101 ctacttccgc gtactggacg agtttgtcgt acatacgctg ggcgaaaacg cgcgccgcca
    20161 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20221 gctggtgaaa gaaaatcgcc gtgatacagg cgatgcctac agctttaact ggtcaatgcg
    20281 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20341 gctttacccg gatcaacctg ttgaagtgct ggctgccgac ctgcgccgtg cg
//
LOCUS       DAJTYM010000072        20317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTYM010000072 DAJTYM010000000
VERSION     DAJTYM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..447)
                     /locus_tag="OO575_002683"
     CDS             complement(<1..447)
                     /locus_tag="OO575_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6665594.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO575_002684"
     CDS             complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO575_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6665595.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO575_002685"
     CDS             complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO575_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6665596.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO575_002686"
     CDS             complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO575_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6665597.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3663..5213
                     /gene="yoaE"
                     /locus_tag="OO575_002687"
     CDS             3663..5213
                     /gene="yoaE"
                     /locus_tag="OO575_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6665598.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5217..6815)
                     /locus_tag="OO575_002688"
     CDS             complement(5217..6815)
                     /locus_tag="OO575_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6665599.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO575_002689"
     CDS             complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO575_002689"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6665600.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8494..9072)
                     /locus_tag="OO575_002690"
     CDS             complement(8494..9072)
                     /locus_tag="OO575_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6665601.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO575_002691"
     CDS             complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO575_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6665602.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10511..10690
                     /locus_tag="OO575_002692"
     CDS             10511..10690
                     /locus_tag="OO575_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6665603.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10810..11169)
                     /locus_tag="OO575_002693"
     CDS             complement(10810..11169)
                     /locus_tag="OO575_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6665604.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11531..11875)
                     /locus_tag="OO575_002694"
     CDS             complement(11531..11875)
                     /locus_tag="OO575_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6665605.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12007..13917
                     /locus_tag="OO575_002695"
     CDS             12007..13917
                     /locus_tag="OO575_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6665606.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13975..14670
                     /gene="tsaB"
                     /locus_tag="OO575_002696"
     CDS             13975..14670
                     /gene="tsaB"
                     /locus_tag="OO575_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6665607.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14710..15291
                     /gene="yeaY"
                     /locus_tag="OO575_002697"
     CDS             14710..15291
                     /gene="yeaY"
                     /locus_tag="OO575_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6665608.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15497..17182
                     /gene="fadD"
                     /locus_tag="OO575_002698"
     CDS             15497..17182
                     /gene="fadD"
                     /locus_tag="OO575_002698"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6665609.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17264..18379
                     /gene="rnd"
                     /locus_tag="OO575_002699"
     CDS             17264..18379
                     /gene="rnd"
                     /locus_tag="OO575_002699"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6665610.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18433..19398)
                     /gene="yeaX"
                     /locus_tag="OO575_002700"
     CDS             complement(18433..19398)
                     /gene="yeaX"
                     /locus_tag="OO575_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6665611.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19454..20071)
                     /gene="yeaW"
                     /locus_tag="OO575_002701"
                     /pseudo
     CDS             complement(19454..20071)
                     /gene="yeaW"
                     /locus_tag="OO575_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         5010 a         5080 c         5269 g         4958 t
ORIGIN      
        1 tacgagaagc ttataaattt tttccagatc gtcgatattt cgaacgcgga ttaacagccg
       61 acgctgttct aattgcatca ccaacacgcc atcttccgac aagttcatcg ctttgattcg
      121 gctatattca atccagacat tggcgaagaa aaaacctttt tgtttaaaga tgatcttcgg
      181 aatgcggatc cagaatatat aaaaacccat cagagccagt gcacttaata accatgttgt
      241 tattagcgcg ccatgattcg tgacgttgtt atagataaga atgacaatca atccgacgaa
      301 gataacgcta tcgatgcgac cacgccggag caaaggaatt gccagcaggg tggggccgtt
      361 acggcggggc atgatgaact gatcgtagat cgcgaaggcc aggagtgcgg cgatgaaaag
      421 aatcagtacc aggtccgtga ttgtcattaa tcctccagat aaaaaaacgg ggccaaaagg
      481 ccccggtagt gtacgacagt cttacagtcc cagcaggccg caagcgtaac cagcgatacc
      541 gatgacgaag aagccaacga tgatccacag cgggttaact tttttgcgca gcagccacat
      601 acaagcaaat gtcagcagca gtggtacaag gcctggcatt aactggtcaa gaatagtttg
      661 cacggtggtg acacgggtct gtccatcctg cccggtgatg gtcgaaacca ccagcgggat
      721 gttgacatgt gtccacttgt taaccaatgc ccccatgaca aacaggccga ggatagacgc
      781 cccttccgtc agtttttgca ggaagccacc acccatatct ttaacgatat cgataccttt
      841 ggagtaacca tacgctacgc cgtagtaacg ggttgccaga cgcaccaggt taaacaggat
      901 gaagaacagc agcggaccta acaggctgcc gctcatcgcg ataccggcac ccagtgctgc
      961 aaataccgga cgtacggttc cccagaagat cgggtcgcct acaccagcca gcggccccat
     1021 caaaccgact ttgataccgt tgatagcacc gtcatcgatc tctgcgccat tagcacgctg
     1081 ttcttccagc gccagggtta cgccgagaat cggtgcagcc acgaacggct gggtgttaaa
     1141 gaactccagg tgacggcgaa tagcttgttt acgagcttcg ttgttctcag ggtagaggcg
     1201 acgaattgcc ggtaccatag agaagcagaa acccagtgcc tgcatacgtt cgaagttcca
     1261 tgaaccctgg aagaggttag aacgcaggaa tacgccacga atatcacttt gagtgagttt
     1321 tttctcggtg gtagtttgag ttgtatcaac catttcgctc acctgttagt ccagttcgtt
     1381 atcgagatcg ttgttaccag ctgcctgagc aggcgcaccg gctacgcggt tgtatttcgg
     1441 gctaagttgg atgtagagca ctgccataac agtaccaatc acacccagag caaccaggtt
     1501 aaagttggtg aatgctgcgg ttacgaagcc gaggtagaag aacggcatca ggtagccagc
     1561 acgcatcatg ttgataacca tcgcataacc aaccaccacg atcatgcccc cggcgatatt
     1621 cagaccattg gtcaccactt ccggaatcgc attcagcatg ttctgtactt cgctggtacc
     1681 aacagacagc gcaacgatga cggccggaat agccacacgc attgcttgca ggaacagaga
     1741 agaaacgtgg atccaggaaa tcgctgtcag gttgccgtta tcagcagcct tatccgcagc
     1801 gtgctggaaa gcaacggtaa tagtacgaac gatgatggtc agtacctggc ccgcagcggc
     1861 cagagggatt gccagtgcga tacctgcacc aatgctctga tgacctgcga taaccagaat
     1921 ggtagaaatg atagaagcca gagcggcgtc aggcgcaact gcagcaccga tgttcatcca
     1981 gcccagcgcg atcatttcca gcgtaccacc gataataata ccggttttca tatccccaag
     2041 aacgataccc accagggtac acgcgattag cggacggtga aactgaaatt catcgaggat
     2101 tgatcccata cctgcgatac aggctacgat aaataccagc acaatttgaa gagtggtaat
     2161 ctccattgta cttctcctgt ttacgtaaga cttaagtgtg aaaactgaat gataatcaac
     2221 acaatacgtt acttatcgat tttgctgatc agatccatca ttttcagttt cggatcggtg
     2281 gaaaccttac ggacttccag ctcaataccg cgtgcattca gttttttgaa cgcctcgata
     2341 tctttttcat caaccgaaac cgcgttattc acctgggttt taccctgacg gaatgccata
     2401 ccaccgacgt taacagaggt gattttcacg ccgccttcaa cgagacgctc tacatctgtt
     2461 gggttggtaa ataacagcat tacgcgttcg ccagcatatt tcgggttgtt gtagacgcga
     2521 atcattttgg caacatcaac tacgtgtgct gttacgcccg gaggtgcaac ctgggtgagc
     2581 agtgtcttac gaacggtatc cgcagccact tcatcactaa caacaataat acgggagaca
     2641 ttggtttctt tggtccagcg ggtggcgacc tgaccgtgaa tcagacggtc gtcgatacgc
     2701 gcaaggccaa taaccatgta gtcgtttggc cccattggtt ttgccggagt tggagccgct
     2761 tttggtgctg cggcagcggg tgctggcgcg gctttttcaa ccggtttggc tttcagtgct
     2821 ttcacgcctt cacggcctgt ttctactgcc agcgcaacca gttcatcaaa gctcgggtca
     2881 tcatcacggg ccattaacgt ttccacgagc attggaatgt taacgcctgc aatgacttca
     2941 taatgctctt tgtcgacgac aatgcggctg gcagcattga acgggctgcc tccccatgta
     3001 tcaacgagaa acagcacgcc tttagtggtg tcgagttttg ccaactgagc gttgtatttt
     3061 tcaatcagcg tttcggcatt ttcacctgga acgaaatcga tccagccgac gttttcctgc
     3121 tcgcctaaca gcatttctgc cgttttaagc aactgctctg cagcccaacc atgtgtgcct
     3181 ataacaatag caatggtcac ttgctacctc ctttattatc gttaacacct caacgtgcca
     3241 gatgtatttt tgaatcgtcg tccacaatcg aatcgattca gataagggct acaaccaaaa
     3301 acccctgcgt ttcgcgattt attttagata tcgaaaaaat tattttatgt gatgaagatc
     3361 cgtaatttaa ctttcgatta tcagaaattt cgaaaggtaa aatatccttg tcacattcgt
     3421 ttgcaaagga aggtaaatct ttgccaaatc agaggcgtct ctgatatgtt taactcccgt
     3481 ttaacaacca tggagtatag ggcagtagcc cgcagtatgg atcgtcaccg acgtcatttc
     3541 agcatcaggc cttttaacgc ctgcctttct ggcactctat gccgcacctt tcgtttgcat
     3601 tttgtcgtta cgcctgcatt atttctggcg tcgaatagct attccttaag caggagcttg
     3661 tcatggaatt cttaatggac ccctcaattt gggcggggct actcacgctt gttgttctcg
     3721 aaattgtgct gggtatcgat aacctggtct tcatcgccat tcttgctgac aaactgccgc
     3781 caaaacaacg cgataaagcg cgtttgctgg ggttatcact ggcgctgatt atgcgtctgg
     3841 ggctgctgtc gctgatttca tggatggtca cgctgaccaa accgctattt accgtcatgg
     3901 atttctcctt ctccggacgc gatctgatta tgttgttcgg ggggatattc ttgctgttca
     3961 aagcaacaac cgaactgcat gaacggctgg aaaaccgcga tcatgattcc ggccacggta
     4021 aaggctacgc cagtttctgg gtggtcgtca cacagattgt catccttgac gccgtcttct
     4081 cgttggatgc ggtaattact gcagtaggga tggttaacca tctgccggtg atgatggcgg
     4141 cggtagtgat tgcgatggcg gttatgttgc tggcatcgaa accgctgacg cgattcgtta
     4201 accagcaccc cacggtggtg gtgctctgtc tgagcttcct gttaatgatt ggtctgagtc
     4261 tggtggcaga aggtttcggt ttccacattc cgaaaggtta cctgtatgcc gcgattggct
     4321 tctcgatcat tatcgaagtg tttaaccaga ttgcgcgtcg caactttatt cgtcaccagt
     4381 cgactttgcc gctgcgagcg cgtactgccg atgccatcct gcgtttgatg ggcgggaaac
     4441 gtcaggccaa tgttcagcac gatgccgata acccgatgcc gatcccggaa ggtgcatttg
     4501 ccgaagaaga acggtacatg attaacggcg tactgacgct ggcgtcgcgt tctctgcgcg
     4561 ggatcatgac gccgcgcggt gaaataagct gggttgacgc taatctcggg gtcgatgaaa
     4621 tccgcgagca actgctctct tcaccgcaca gtctgttccc ggtatgtcgc ggtgaactgg
     4681 atgaaatcat cggtatcgta cgtgctaaag aactgctggt ggcgctggaa gagggcgttg
     4741 atgtggcggc gattgcttcg gcgtctccgg cgattatcgt cccggaaacc ctcgatccga
     4801 tcaacctgct gggcgtgctg cgtcgcgctc gcgggagctt tgttatcgtg accaacgagt
     4861 ttggtgtggt acaaggcttg gtcacgccgc tggatgtgct ggaagccatt gcgggtgaat
     4921 tcccggacgc tgacgaaacg ccggaaatca ttaccgatgg taacggctgg ctggtaaaag
     4981 gcggtacaga tttgcatgcc ttgcagcagg cgcttgatgt tgagcacctt gccgatgacg
     5041 atgatatcgc gacggtcgcg ggcctcgtga tctcggcaaa tggtcacatt ccccgtgtgg
     5101 gcgatgtgat tgatgtaggg ccactgcata tcaccatctt tgaagccaat gattatcgtg
     5161 ttgatctggt tcgcattgtt aaagagcaac cggcgcacga tgaagatgag taagcgttaa
     5221 cgtaacgaca taatgggcgt gatatgtcca ttatgccggg cgggcggcgg ttggctgccc
     5281 gccagccatt tgggaaaatc acgtagcggc atcggctgtg cgtacaaata cccttgcaaa
     5341 atatgcacgc catgacggcg caaatacttc gcctgttctt gcgtttccac gccctccgcc
     5401 accagttcaa tattcagtct ttgccccagc gcgatgatga tatcggtcac cgtcgagtta
     5461 accgcgtcag aacctatagc tgcggtaaat gacttatcaa ttttcagcac gtcaggacgt
     5521 aatgtttcaa gccaggaaaa cgagctgttg ccggtgccga agtcatcaat cgccagtttg
     5581 acgtttttac gatgcagctc gcgggcaatc cgataatcaa catccagtaa ggcatcgcgt
     5641 tcggtgattt cgaggatcag ttgctgaatc gggtgagcgc taaaccagta ctgattgaga
     5701 tcttttatca gcacaccacg gcgaaaatgg ctgggtgcga cgttaatgcc aacatgaaac
     5761 tgactactca tcgggaaaac atggtgctga cgaatggttt ctgccatcac atagtgggtc
     5821 agtggcacaa ttaaatgatg ttcttccgcg ataggaataa acacatccgg tgaaatccag
     5881 ccctgacgcg gattattcca gcgcagcaaa atctctacac caatacattg ctggctgcgc
     5941 gcattaagca aaggctgaca gaacaattcg aactcatgtt gcgccaggcc cagattaatt
     6001 tcgcgggaaa agctcatccg gtaagcggtc gccagccagg cgatatagcc caccagtaaa
     6061 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
     6121 cccggaccat ttacgttaat ggtaaaagga aagctttgcg aagaaacctg atagttttca
     6181 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
     6241 tccaccgtca ggctggcgct actgatttgc ggcagttgtg gctcaaggag cattgccgtc
     6301 agtaagtcga tgttaatcat ctctatgacc ccagcattgc tactgcccgc tgcaggcgtc
     6361 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
     6421 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
     6481 atgctggagc aataaagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
     6541 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
     6601 agcaagactt tgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
     6661 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
     6721 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgg
     6781 cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc
     6841 gacaaacgcc tgacgcaaca gtggaagtgt agtggggaat attacagcag acgagaaagc
     6901 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
     6961 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
     7021 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     7081 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     7141 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     7201 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     7261 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     7321 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     7381 gtctgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     7441 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     7501 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     7561 gctggaaact aacatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     7621 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     7681 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     7741 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     7801 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     7861 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     7921 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     7981 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttt
     8041 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     8101 aagacccata ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     8161 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc
     8221 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     8281 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     8341 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     8401 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     8461 agtagtgttt tgtagatgta aagataagta tagtcagggg gtcacaccaa tttgcagcgc
     8521 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     8581 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     8641 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     8701 cggcagatcg ggcgggataa tgccgaccac tggggttact tgatagccag ttacgctatc
     8761 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     8821 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     8881 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     8941 cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttaggggttc
     9001 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     9061 acggtattcc acgtcttact tctccagttg gcgtaatatc ttattaactt tatcaaaagt
     9121 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     9181 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     9241 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     9301 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     9361 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     9421 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     9481 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     9541 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     9601 gcgtggtagc gtgcctttaa tcgggcgagt ctggatttca ctattatcac aaagaataaa
     9661 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     9721 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     9781 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     9841 ctggcgaaat ttttcgccgt actgctcgtg ggtcatattg gattgccagt cggaagtgag
     9901 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     9961 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
    10021 gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
    10081 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
    10141 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag
    10201 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttacctaa
    10261 agtggttaaa gtgcaaatcg gatcggcgac cacaatatca aagcggctat acggatgatc
    10321 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
    10381 ttcagcggcg tcctgacgcc agggtaaagt aatcacagtg ggagataacg tcttcatcag
    10441 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
    10501 ggagttaaca atgtttgcag gtttaccttc actcatccat gaacagcagc aaaaagctgt
    10561 cgagcggatc caggaactga tggcacaagg gatgagtagc ggccaggcaa tcgcgctggt
    10621 ggcagaagag ctgcgtgcca accactccgg cgagctaatc gtggcgcgtt ttgaggatga
    10681 agacgagtaa cacactaatc cgtatcgatt tcgttataga agccgatgat catttgattg
    10741 tgagaaaaag gacgcttttt atatgtgatt aaaagcgtcc tgacccattt atgctgatat
    10801 aaatttaact cagaccagtg cttttagttc ttcctgcata tatgttgaaa agtattcagg
    10861 atttttaatc acagagcgtt cacctgtctc catttttttt gcccatccaa ccattttttc
    10921 cgtgaaccca gggttccagc cttcctggtc agctcgcgcc gtaatatcag cggcactcgc
    10981 gggtactccc agttctttta aatatttaaa gattcccttc gccgtgcttt cattcatcga
    11041 acttggtgtt ggcgctgata catccatggc accaatgaaa tccagggctt tatctattac
    11101 agcaggcata ttcttatcct taaaattaac catgttatcg ataaggctat atatgacctt
    11161 aatttgcatc taatatcaaa gggattataa cgatatggca cgagttaacc tgttgacatt
    11221 caggtgtttt aaaggtagca gctcatggtg tgttatccag taagatggag aaaattaacg
    11281 gtcagcccgc acgctataac cagattgttc agctaagaaa gaaaaccaga gttgctaatc
    11341 aaagggcgaa cacaacggca tggttatgtt tatttatata gttagtttta tagataaaat
    11401 tgaaattata aatataaata atgaattagc agaagcatca acagtaatat cttattctct
    11461 ggtcgattta tgtaaaaccg accagtatta tcgataaatt atcgtctgac ttattttgct
    11521 tcgataaagc ttacaccgca gccacaatct taatttcaac tttatacttc gggttcatca
    11581 aacccgcttg taccgtgcag cgcaccggcg catgacccgc gacaacccag gcatcccacg
    11641 ctttattcat cgccgcgaag tcgtttttat cggccaggaa aatggtggca tccagaatgc
    11701 tcgatttatt gctgccctgt ttttccagca cggcgtcaat ctgtgccagc gtgttggcgg
    11761 tttgctcaaa ggcatcggcg tcgaggtttt ccggtacacc agtgtagtag agcgtgttgt
    11821 tgtggattac tacatcagac cagcgggctt cagcatcgat acgaacgata gtcatcattt
    11881 tgtcctcatt atacttccat tttcgtgctg caaagactgc cacaatgtga ggttgccgtc
    11941 accacttcaa ctggcgaaag cgccctcatc ctgacataat ccctgttcaa atcaacaggg
    12001 ggtagtgtga cggacgattt tgcaccagac ggtcagctgg cgaaagcgat accaggcttt
    12061 aagccgcgag aaccacagcg acagatggcg gtagccgtca cccaggcgat agaaaaaggc
    12121 cagccgctgg tggtggaagc aggaaccggt acgggcaaaa cctacgctta cctggctcct
    12181 gcgctgcggg cgaaaaagaa agtcattatc tcgaccggct caaaagcgtt gcaggatcag
    12241 ctctacagcc gcgatttgcc aacagtctca aaggcattga aatatacggg caacgtggcg
    12301 ctgctgaaag ggcgctcaaa ctacctctgc ctcgaacgtc tcgaacagca ggcgctggcg
    12361 gggggcgatc tgccggtaca aatcttaagc gatgtgatcc tgctgcgctc ctggtctaat
    12421 caaacagtcg atggtgatat cagcacctgc gtcagcgtgg cggaagattc acaggcgtgg
    12481 ccgctggtca ccagcaccaa cgacaactgt cttggcagcg actgcccgat gtataaagat
    12541 tgctttgtgg tcaaagcacg taaaaaagcg atggacgccg atgtggtggt ggtaaaccat
    12601 catctctttc tggcggatat ggtggttaaa gagagtggat ttggcgagtt gatccctgaa
    12661 gcggacgtca tgatcttcga cgaagcccac cagctaccgg acattgccag ccagtatttt
    12721 ggtcagtcac tctccagtcg acaactgctc gacctggcaa aagacatcac catcgcctac
    12781 cgcaccgaat taaaagacac ccagcagtta caaaagtgcg ctgatcgtct tgcccagagt
    12841 gcgcaggatt ttcgtctgca actcggtgag ccaggttatc gcggtaacct gcgtgagctg
    12901 ttagctaatc cgcaaattca gcgggcattt ttactgctcg atgacacctt ggaactttgt
    12961 tatgatgtgg cgaaactgtc actggggcgt tccgccttgc tggatgcggc atttgagcgc
    13021 gccacgttgt atcgcacacg gctgaagcgg ctaaaagaga tcaatcagcc gggctacagc
    13081 tactggtacg aatgcacttc gcgccatttt actctggctc tcacgccgct cagcgtggcg
    13141 gataaattca aagagttaat ggcgcaaaag cccggtagct ggatcttcac ctcagcaacg
    13201 ctgtcggtga acgacgatct gcatcatttc acctcgcggc ttgggataga acaggcggag
    13261 tcgttgctat tgccaagccc gtttgattac agccgccagg cgttactctg tgtgccgcgc
    13321 aatctgccgc aaaccaacca gccaggttct gctcgccagt tagcggcaat gctgcgaccg
    13381 atcatcgaag ctaacaacgg tcgttgtttt atgctttgta cctcgcacgc catgatgcgc
    13441 gatctggccg agcagttccg cgctaccatg acgcttcccg ttttgttgca gggggaaacc
    13501 agtaaaggcc aactgttgca gcagtttgtc agtgccggta atgcgcttct tgtggcaacc
    13561 agtagtttct gggaaggggt ggacgtgcgt ggcgatacat tgtcattggt aattatcgac
    13621 aaattgccgt ttacctcgcc ggatgatcca ctgttaaaag cgcgcatgga agattgtcgt
    13681 ttgcgcggtg gcgacccgtt cgatgaagtg caactaccag atgccgtcat tactctcaaa
    13741 cagggggtag gtcgactgat tcgcgacgcc gacgatcgtg gcgtgctggt gatttgtgac
    13801 tatcggctgg tgatgcgtcc ttacggcgcg acgtttctcg ccagtctgcc gcccgcgcca
    13861 cgcacccgtg acattgcccg tgcggttcgt ttccttgcga taccatcctc caggtaattt
    13921 gttacggagt atggtatgat gcgcgccttt ttattgatct aagcacgaag acccatgcga
    13981 attctggcta tcgataccgc gacagaggcc tgctctgtcg ccctgtggaa cgacggtact
    14041 gtcaacgctc attttgagct ttgccctcgt gaacatactc aacgaatctt accgatggtg
    14101 caggatatcc tgaccaccag cggaacttcc ctgactgata ttaacgctct ggcttacggg
    14161 cgcggccccg gtagctttac cggcgtgcgc attggtattg gcatcgcgca agggctggcg
    14221 cttggcgcgg aattaccgat gattggcgtc tccacgctaa tgacgatggc gcaaggggcg
    14281 tggcgcaaaa acggcgcaac acgcgtgctg tcagccattg atgcgcgaat gggcgaagtt
    14341 tactgggccg aatatcagcg tgatgaaaac ggtatctggc acggtgaaga aaccgaagcc
    14401 gtactcaaac ccgaactcgt tcatgaacga atgcaacagc tttccggcga atgggtgacg
    14461 gtagggacgg gctggcaagc ctggccggat ctcggtaaag agagcgggct ggttttgcgc
    14521 gatggcgaag tgttactccc cgctgcagaa gatatgctgc cgattgcttg tcagatgttt
    14581 gccgagggta aaacggtggc ggtggaacat gccgagccgg tttatttgcg taacaacgtc
    14641 gcatggaaga aacttccggg caaagaatga atcttaagag tatgccctga gaaaaaaagg
    14701 agtcgcatca tggcggttca aaagaatgtt atcaaaggca tactggcagg tacgtttgcg
    14761 ctaatgctga gcggttgtgt cactgtgccg gacgccatta aaggcagcag taccacgccg
    14821 caacaagatt tagttcgggt gatgagtgcg ccgcagctgt acgttggtca ggaggcacgc
    14881 tttggtggca aagtggttgc ggtacaaaac cagcaaggga aaacccgcct ggaaattgct
    14941 accgtaccgc tggacagcgg agctaggccg acgctgggag aaccttctcg cggtcgcatt
    15001 tatgccgatg tgaacggttt tctggacccg gtggatttcc gtggacaact ggttacggta
    15061 gtcgggccaa tcaccggtgc ggttgacggc aaaatcggca atacgcccta taaatttatg
    15121 gtgatgcaag taacgggtta caaacgttgg catttaaccc agcaggtgat tatgccgcct
    15181 cagccgattg atccatggtt ttatggcggt cgtggctggc cctatggcta cggcggatgg
    15241 ggctggtata atcccggccc cgcgagagta caaacagttg taaccgaata attgcttgtt
    15301 tttaaagaat gcagaaacag cggctggtcc gctgtttctg cattcttacg gtaaagataa
    15361 aaataaatag tgacgcgctt cgcaaccttt tcgttgggta attatcaagc tggtatgatg
    15421 agttaatatt atgttaacgg cgtgtatatc atttggggtt gcgatgacga cgaacacgca
    15481 ttttagaggt gaagaattga agaaggtttg gcttaaccgt tatcccgcgg acgttccgac
    15541 ggagatcaac cctgaccgtt atcaatctct ggtagatatg tttgagcagt cggtcgcgcg
    15601 ctacgccgat cagcctgcgt ttgtgaatat gggggaggtg atgaccttcc gcaagctgga
    15661 agaacgcagt cgcgcgtttg ccgcttattt gcaacaaggg ttggggctga agaaaggcga
    15721 tcgcgttgcg ttgatgatgc ctaacttatt gcaatatccg gtggcgctat ttggcatttt
    15781 gcgtgccggg ataatcgtcg taaacgttaa cccgttgtat accccgcgtg agcttgagca
    15841 tcagcttaac gatagcggcg catcggcgat tgttatcgtg tctaactttg cccacactct
    15901 ggaaaaagtg gttgataaaa ccgccgttca gcacgtaatt ctgacccgta tgggcgatca
    15961 gctatctacg gcaaaaggca cggtagtcaa tttcgttgtt aaatacatca agcgcttggt
    16021 gccgaaatac catctgccag atgccatttc atttcgtagc gcactgcata acggctaccg
    16081 gatgcagtac gtcaaacccg aactggtgcc ggaagattta gccttcctgc aatacaccgg
    16141 cggcaccaca ggtgtggcga aaggcgcgat gctgactcac cgcaatatgc tggcgaacct
    16201 ggaacaggtc aacgccacct atgggccgtt gctgcatcca ggtaaagaac tggtagtaac
    16261 ggcactgccg ctgtatcaca tttttgccct gaccattaac tgcctgctgt ttatcgaact
    16321 gggtgggcag aacctgctta tcactaaccc gcgtgatatt ccagggttgg taaaagagtt
    16381 agcgaaatat ccgtttaccg ctatcactgg cgttaacacc ttgttcaatg cgttgctgaa
    16441 caataaagag ttccagcagc tggatttctc cagtctgcat ctttccgcag gcggtgggat
    16501 gcctgttcag caagtggtgg cagaacgctg ggtgaaactg accggacagt atctgctgga
    16561 aggttatggc ctgaccgagt gcgcgccgct ggtgagcgtt aacccgtatg atattgatta
    16621 tcatagcggt agcatcggtt tgccggtgcc atcgacggaa gccaaactgg tggatgacga
    16681 cgacaacgaa gtaccaccgg gtcaaccggg tgagctttgt gtcaaaggac cgcaggtgat
    16741 gctgggttac tggcagcgtc cggatgctac agatgagatc atcaaaaatg gctggttaca
    16801 caccggcgac atcgcggtga tggatgaaga aggattcctg cgcattgtcg atcgtaaaaa
    16861 agacatgatt ttggtttccg gttttaacgt ttatcccaac gagattgaag atgtcgtcat
    16921 gcagcatcct ggcgtacagg aagtcgcggc tgttggcgta ccttccggct ccagtggtga
    16981 agcggtgaaa atcttcgtag tgaaaaaaga tccatcgctt accgaagagt cactggtgac
    17041 tttttgccgc cgtcagctca cgggatacaa agtaccgaag ctggtggagt ttcgtgatga
    17101 gttaccgaaa tctaacgtcg gaaaaatttt gcgacgagaa ttacgtgacg aagcgcgcgg
    17161 caaagtggac aataaagcct gagcgttaag tcagtcgtca gacgccggtt aatccggcgt
    17221 tttttttgac gcccactaaa gagaaaacaa tttgaattac caaatgatta ccacggacga
    17281 tgcgctggct tctttgtgtg aagccgtccg tgtctttccg gcgatagccc tggatactga
    17341 atttgttcgt acgcgcactt attacccgca gctggggttg attcaacttt tcgatggcga
    17401 gcatctggcg ctaattgatc ctctcgggat caccgactgg tcaccgctga aagcgatcct
    17461 gcgcgatccg tccatcacaa aatttctcca tgcaggcagt gaagatctgg aagtgttcct
    17521 caatgtcttt ggcgaattac cacaaccctt gattgacacg caaatccttg ctgccttctg
    17581 cggacgcccg atgtcatggg gtttcgcttc catggtggaa gagtattccg gcgttacgct
    17641 ggacaagagt gaatcgcgca ccgactggct ggccagaccg ctgaccgaac gtcagtgtga
    17701 atacgcagcg gcggatgtct ggtatctgtt accgatcacc gccaagctta tggtagaaac
    17761 ggaggcctcc ggctggctac ctgcggcgct ggatgaatgc cgcctgatgc aaatgcgtcg
    17821 tcaggaagtc gttgcgccgg aagatgcctg gcgtgatatc accaatgcct ggcagttacg
    17881 cacacgccaa ctggcctgtc tgcaactgtt agccgactgg cgactgcgca aggcgcgaga
    17941 gcgtgatctg gcggtgaact ttgtcgtgcg tgaagagcat ttgtggtcgg tcgcgcgtta
    18001 tatgccggga agtttaggcg aactggacag cctgggttta tccggtagcg aaatccgctt
    18061 tcacggtaaa acgctgctgg cgctggtgga aaaagcgcag acattgccgg aagatgcctt
    18121 accgcagccg atgcttaacc tgatggacat gcctggttat cgtaaagcgt ttaaagcgat
    18181 taagtcgctg attactgacg tgagcgaaac gcataagatc agcgccgaat tgctggcatc
    18241 gcgtcggcaa atcaaccaac tgctgaactg gcactggaaa ctgaaaccgc agaacaattt
    18301 gccggagctg atttccggct ggcgtggtga gctgatggcg gaagcattac acaatttatt
    18361 gcaggaatat ccgcagtaaa atcttccgaa gccggactgg gcgcgctcag cgccacatcc
    18421 ggctttggca aactacaaat ccaacaccag gcgtttaccc ttcgcacgcg aacaacagat
    18481 caacatactt tgctggctgg cacgctcttc atcgctaaaa tactgatccc gatggtcagc
    18541 ttcaccttcc agtattgccg tttcgcaggt tccgcatacc ccttcacgac ataaacattc
    18601 cactttcgcg gctttattat tttcaataac ctgcaaaata gtcatctctt ccggcaccac
    18661 aaactctttt ccggaacggg caagcaccag ggtaaatgca tcgccggttt tgtcttcgat
    18721 agcaaattgc tcaaagtgca gtgtatcggc ggcgatgtcc agacgcgcag cttcacttct
    18781 taccgcttca attagcgcct cggggccaca ggtataaacg tgtgtgccag gttcgatatc
    18841 cgccaataat cgcgctaatt ccagtcgtgt tccggttgat gaatgatgca aatggacttt
    18901 ctcagcctgc ggatgctgga ctagctcagc acgaaatgcg caactttctg gatttcgcga
    18961 gcagtaatgt agctgccagt cgatgtcgct gtgttgcaac tctgccatgt gcgacaggaa
    19021 aggggtgata ccaataccgc ccacgataaa cagatgcttt ctggctgagg gaattagcgc
    19081 aaacaggtta ttaggcgttg agatcgttaa ccgatcgccc acttttacct gctgatgcaa
    19141 aaagcgggaa ccgccgcgcg agttttcttc cagtcgaacg gcaatctgat aactagaggt
    19201 gttatgcggt gaactcagta gtgaatacgc attgctgtac tggttatcac catcgctcat
    19261 ctgcactagg atgtgacttc ctccggtgaa cgcaggcagc ggtttgccat cggttgccac
    19321 cagcgtgaag cgtttcacct gttcggtaag gggttcaacc tggctcacct gtacttcaaa
    19381 catttgatag tctgacattg ctgtactcca tcaaaaatca gcccagcggc ggaaaatacc
    19441 gccgccgatg tcattagtcc ttaaacacct gcgccagcag attatggaaa tgggcgatac
    19501 catgttcgga aatgccacta ccgctactgt cggccatgat gcgcccctga ccacgatagc
    19561 cacgcgattt cagccctttc tgtacgcttt caaccagacg taaatcttcc ggatggaaca
    19621 catcgcgata ccactcaatc agcgattttt gctcgtcggt taactcttca ttggtgaagt
    19681 aaatatcgta gttttgcagg gtagtttcag aatccaccgg gaattcataa atgaccgtca
    19741 tcatcccttt gatcggggtg acgttcagca tcgtgcacgg ccacagccag aaaccgtgga
    19801 atgccgcatc cgtaccctct tcaaatttaa acgactgttc ggacggtttg gcgaaaccgt
    19861 attgcagcgt ccagttaccg tgcatggtgt gccagtaacg atcaacctgt acggagtcgg
    19921 agaaacctag atgcgccgga ccacagtgat agcactcgag atagttatcg acaatgttct
    19981 tccagttggc aggcgtgcgg gtggtaaagc gggccgctag tttcagattg tggacttccg
    20041 ggcaggcttc cagcactttc gcgcccagac cggtgtattg attcacttga agtacgaaaa
    20101 aaaaccggga ggacattgga ttattcgggt tctgatggga ttagatttgg tggggcttgc
    20161 aagcctgtag cgcaaatttt agtcgttaat caatgaaacg cgaaagatag taaaaaattg
    20221 cttttgtttc attgaaaata cgaaaaacaa aaacactgca aatcatttca ataacagctt
    20281 caaaaaacgt tcaaaaccga taacaaccag gctgtaa
//
LOCUS       DAJTYM010000073        20219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTYM010000073 DAJTYM010000000
VERSION     DAJTYM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..569
                     /locus_tag="OO575_002702"
     CDS             <1..569
                     /locus_tag="OO575_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6665612.1"
                     /translation="NAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQR
                     AKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHA
                     QKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDF
                     SHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            587..1225
                     /locus_tag="OO575_002703"
     CDS             587..1225
                     /locus_tag="OO575_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6665613.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1516..2244
                     /gene="mpaA"
                     /locus_tag="OO575_002704"
     CDS             1516..2244
                     /gene="mpaA"
                     /locus_tag="OO575_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6665614.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2219..3184)
                     /gene="ycjG"
                     /locus_tag="OO575_002705"
     CDS             complement(2219..3184)
                     /gene="ycjG"
                     /locus_tag="OO575_002705"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6665615.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3303..3809
                     /gene="tpx"
                     /locus_tag="OO575_002706"
     CDS             3303..3809
                     /gene="tpx"
                     /locus_tag="OO575_002706"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6665616.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3853..5394)
                     /gene="tyrR"
                     /locus_tag="OO575_002707"
     CDS             complement(3853..5394)
                     /gene="tyrR"
                     /locus_tag="OO575_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6665617.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5542..6603)
                     /locus_tag="OO575_002708"
     CDS             complement(5542..6603)
                     /locus_tag="OO575_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6665618.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6600..7997)
                     /locus_tag="OO575_002709"
     CDS             complement(6600..7997)
                     /locus_tag="OO575_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6665619.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8153..9151
                     /locus_tag="OO575_002710"
     CDS             8153..9151
                     /locus_tag="OO575_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6665620.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9262..10134)
                     /gene="ompG"
                     /locus_tag="OO575_002711"
                     /pseudo
     CDS             complement(9262..10134)
                     /gene="ompG"
                     /locus_tag="OO575_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10207..11288)
                     /locus_tag="OO575_002712"
                     /pseudo
     CDS             complement(10207..11288)
                     /locus_tag="OO575_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11302..11961)
                     /gene="pgmB"
                     /locus_tag="OO575_002713"
     CDS             complement(11302..11961)
                     /gene="pgmB"
                     /locus_tag="OO575_002713"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6665621.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11958..14225)
                     /gene="ycjT"
                     /locus_tag="OO575_002714"
     CDS             complement(11958..14225)
                     /gene="ycjT"
                     /locus_tag="OO575_002714"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6665622.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14222..14845)
                     /locus_tag="OO575_002715"
     CDS             complement(14222..14845)
                     /locus_tag="OO575_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6665623.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14842..15264)
                     /locus_tag="OO575_002716"
     CDS             complement(14842..15264)
                     /locus_tag="OO575_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6665624.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15286..16061)
                     /locus_tag="OO575_002717"
                     /pseudo
     CDS             complement(15286..16061)
                     /locus_tag="OO575_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16079..17131)
                     /locus_tag="OO575_002718"
     CDS             complement(16079..17131)
                     /locus_tag="OO575_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6665625.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17162..18004)
                     /locus_tag="OO575_002719"
     CDS             complement(17162..18004)
                     /locus_tag="OO575_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6665626.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(17991..18872)
                     /locus_tag="OO575_002720"
     CDS             complement(17991..18872)
                     /locus_tag="OO575_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6665627.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18893..20176)
                     /locus_tag="OO575_002721"
                     /pseudo
     CDS             complement(18893..20176)
                     /locus_tag="OO575_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
BASE COUNT         4914 a         5542 c         4947 g         4816 t
ORIGIN      
        1 ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg ggtgttaata
       61 tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta ttgaatgcaa
      121 tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac gaattacttc
      181 ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt tatgaagcaa
      241 ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa atgttgttct
      301 cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg ttactgaccc
      361 agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat cgtgatggac
      421 tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg ttacccgatt
      481 tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg gcaaaagtta
      541 ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga aaaatgtact
      601 gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg cagataaaca
      661 aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac cctatccaac
      721 taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc aggccatgca
      781 gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc aggcaaattc
      841 agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc tttccctcgg
      901 tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta ttggtgaacc
      961 cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg aatatacaat
     1021 tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta cctctcgcaa
     1081 tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa ttacagatat
     1141 catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac ccgggactga
     1201 tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac tgtactatgt
     1261 gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga ttaacatgtg
     1321 tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc taatatctgc
     1381 acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg atgacatagc
     1441 gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt aatacttcct
     1501 gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc atttccgccc
     1561 ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt tcctgcccct
     1621 gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga aaactcttcg
     1681 gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg ccatcatgtg
     1741 gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc taatggtgtg
     1801 gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt ttatcgctgg
     1861 aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc tggctccgaa
     1921 cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg ggtggtctca
     1981 ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg cgaatggctg
     2041 gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc tggctctttc
     2101 ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc gcccatctcc
     2161 tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg ctggcatcct
     2221 aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc gccagccagg
     2281 tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg gcgctaatgg
     2341 cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca cgcgcttcag
     2401 tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg ttaaccatct
     2461 catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca tcagcacaaa
     2521 tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga agcggttgtt
     2581 caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac ccttctgcac
     2641 gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg cgaattgcca
     2701 ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc gcgcctgcct
     2761 gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca accgtctgtg
     2821 cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat tgctgctgtt
     2881 ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg ccggcaggga
     2941 gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg acgctcataa
     3001 tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg cattcgccgg
     3061 tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca ctgcgacttc
     3121 cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc ttaacgattc
     3181 tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag cgtttgctgt
     3241 gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca ggaaagataa
     3301 tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat tccatcccgc
     3361 aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct gacgtcaccc
     3421 tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt gataccggtg
     3481 tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac aacaccgttg
     3541 tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc gcagaaggtc
     3601 tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa gcctacggtg
     3661 tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg gttattgacg
     3721 aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag ccggattacg
     3781 aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt gcaccatcag
     3841 gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc gcaacttatt
     3901 ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc tgggataatt
     3961 gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt ccagcgaacc
     4021 ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca acaaaatatc
     4081 ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag cgttctttaa
     4141 ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt cagcggccag
     4201 tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct cagttaacgg
     4261 cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca cgttcagacg
     4321 ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat tcttctgcgt
     4381 agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc gacggaaagt
     4441 gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt cccctatttc
     4501 atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc cttccggagc
     4561 atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat tcagcgccag
     4621 gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa gatctttacc
     4681 tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt tctgcgcctg
     4741 ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg cgctgacgtc
     4801 ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca acatcaccac
     4861 cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg taatctccat
     4921 caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg gttcgctttc
     4981 cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt tgcgcaggcg
     5041 atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca ctttgctttt
     5101 catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca acgccagatg
     5161 ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg caatacggcg
     5221 tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat tgaggtagat
     5281 tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca cgagtagatc
     5341 gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac gcatgggaac
     5401 cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat agcaggaagg
     5461 tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg taaagatatc
     5521 cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt gcagagtttc
     5581 tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt catcgtcaat
     5641 ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg tcagaagtcc
     5701 agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt gcgacatcca
     5761 gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat tcagcaatac
     5821 cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca gcgtggcaat
     5881 gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca acgccagcgg
     5941 gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc gccgcgcctg
     6001 ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac gatcgttctg
     6061 cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat caatacccgc
     6121 ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc tatgcaataa
     6181 atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc gccgccactc
     6241 tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac atccacccag
     6301 cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct gcccgacaac
     6361 gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc tgcgtttcgg
     6421 acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg cttcgtcgag
     6481 cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg ccctgaattt
     6541 tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta acggttcggt
     6601 catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat atgcggcagc
     6661 ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc ttgcttgtcc
     6721 cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt gagaagcgca
     6781 ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac gtcaataatg
     6841 ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat cctttcaaac
     6901 gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat attggcgtgc
     6961 tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc gataaccggc
     7021 gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat cagctgcgtc
     7081 agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg caggcaatcc
     7141 accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc cttcaccact
     7201 ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg cttatcggcc
     7261 tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa ttgcagcgcg
     7321 ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa gtgcagcccc
     7381 tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc cagacggttt
     7441 tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt cgccgaccat
     7501 tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa gtaatcctgc
     7561 gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc cagatacagc
     7621 gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg taacgccagg
     7681 cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc atataactgc
     7741 gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg aatgcgtttt
     7801 acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc tccggcatga
     7861 atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct gcggctaagt
     7921 ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc attgagttca
     7981 tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa aaatcattcg
     8041 taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt gtcatcgacg
     8101 ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac ctatgtcccc
     8161 tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat cacgcgtgct
     8221 gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg ccattgaaga
     8281 attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt ttgatgccat
     8341 tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt tctctgaagt
     8401 tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat tacaaacgtc
     8461 gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga aaatgattaa
     8521 aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac tcgataaata
     8581 cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg tttattctgt
     8641 cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg aaagtgggca
     8701 tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg atcgcgttaa
     8761 tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat ggattgttga
     8821 cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa gtcagtcacc
     8881 gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt atcgtgcggc
     8941 ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata gtaatgaaac
     9001 gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc aggagttagg
     9061 tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac cacagaatat
     9121 taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg aatgccggat
     9181 gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta ctcgcgccgc
     9241 atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg cataatggaa
     9301 tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca gcgaaacgga
     9361 aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat catggccttc
     9421 acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa tgcgcgtata
     9481 cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt cttgtgtgga
     9541 aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat aatagttcac
     9601 tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt cgacacgggt
     9661 atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca accaaccgtt
     9721 gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc agcgctgcat
     9781 attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac ggaaaccgcc
     9841 ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct ccagctccgg
     9901 gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt cctgataata
     9961 ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag gctccgccag
    10021 cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg caccgatatt
    10081 aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac acatcaccag
    10141 tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa tgccttcccc
    10201 ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt tcgtcatatc
    10261 aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag cacggaccac
    10321 taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa ttctgccacg
    10381 ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc ttccgggcgt
    10441 attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc taatttctct
    10501 tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat cgtgccgcga
    10561 ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc gggttggtta
    10621 taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat cacaatccgc
    10681 gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt ggtgttcagt
    10741 ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc atcaaggtta
    10801 gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg cccgagcgcc
    10861 acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta ctcacgcagg
    10921 ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc gattttttgc
    10981 atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa cgcgtagttc
    11041 tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg tttgccgtcg
    11101 atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag ggtggtcgat
    11161 ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat ttccaggttg
    11221 aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg taacgaaagc
    11281 tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc gcgaccaggt
    11341 cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac cgatccccac
    11401 cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt caatgccgat
    11461 acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt ccggatccgg
    11521 tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac gcagctccag
    11581 cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg gaatctgctg
    11641 tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa ccgtcagctc
    11701 gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc gctcctgcgg
    11761 gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact catcgcggct
    11821 gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt cagcggcaat
    11881 ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat ccagatcgaa
    11941 aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc attgaagggc
    12001 aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag ctgaccgtcc
    12061 aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc gagagtgacc
    12121 tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg ctcaagtaac
    12181 gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc ctgaatcgcc
    12241 cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact atgcggatca
    12301 gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt cagcaggcca
    12361 cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg aatagtgcgc
    12421 ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc tggcagcatg
    12481 taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac ttctgcgcgt
    12541 gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc cagattaatc
    12601 gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg aatttctggc
    12661 agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc actacagcca
    12721 aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata gctggtgaag
    12781 gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg aatttccaga
    12841 cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag aagtagcgcc
    12901 atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg aataaccgcc
    12961 caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt ttgccgcagc
    13021 ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct gcgcgcgctt
    13081 tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg cgcgcctggc
    13141 aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct aaacagatgg
    13201 aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc ttcgccggtt
    13261 aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat gcgcagatga
    13321 taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac cgtgatacgg
    13381 cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg gtcgtaactc
    13441 tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc taaagcagct
    13501 tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt ctcgcctgca
    13561 tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata gcattgctgc
    13621 acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc atcctgggtg
    13681 gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc gtcgagatgc
    13741 tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac tgacgcgtcg
    13801 gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc tgccgaaaca
    13861 aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac aacgctacgg
    13921 cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga taacgagaaa
    13981 acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac cagttcgttg
    14041 atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg cgtctggcgc
    14101 gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg cgccatcagc
    14161 gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt tactggcctg
    14221 gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc accagttgct
    14281 ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt acgtgattga
    14341 taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt tgcatcagcg
    14401 tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc gcaccggctt
    14461 tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt gcaaatgacg
    14521 tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc gaatcttcga
    14581 cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc gtgccgatct
    14641 tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca tacatcgcag
    14701 catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt tctttattgg
    14761 taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg gtaacgtaaa
    14821 tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg agcgcaaacg
    14881 atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc acatttccca
    14941 cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc ctgcttccag
    15001 tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa ccacatcagg
    15061 tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc cgtatttttc
    15121 tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt ccagatcgtt
    15181 gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc ctattatcgc
    15241 gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc aggtacgcaa
    15301 ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac gcccttcata
    15361 caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt ggaaatcgag
    15421 ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac cgagcaagtc
    15481 acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt gatagaaatc
    15541 gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag catcggcgag
    15601 ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca cggttccggt
    15661 acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc ggtcgccgtc
    15721 caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc acgctgccgg
    15781 aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa gccattaaga
    15841 cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc ggtggtcact
    15901 ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac cagcagtttg
    15961 ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct ctcgcggata
    16021 ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt cctttgaatt
    16081 aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac tgcatgtagc
    16141 tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa ttgagataac
    16201 cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag cgcggataat
    16261 ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg aaatgcgctt
    16321 cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag gagatggtgc
    16381 cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg ctggtttcga
    16441 taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct acgtcagtgc
    16501 caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag cgatgggcga
    16561 taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg atggcaattt
    16621 gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg ttggcatcgc
    16681 gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc caggagctgc
    16741 cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc gtctcggaga
    16801 gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg ctgccgcact
    16861 cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca atgccgcgcg
    16921 gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct tcatcgataa
    16981 acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg ccgaaacgca
    17041 cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc agcgcagcaa
    17101 cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga aactggccgc
    17161 tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc aaaagcgcat
    17221 acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt ccccagatgt
    17281 aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg ctggcgctgg
    17341 agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag tagacgaaca
    17401 ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt tgcagacggt
    17461 tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg aagtaacttt
    17521 tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc agcgccatct
    17581 cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc agcaaaatgc
    17641 cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct ttaaagcgca
    17701 ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg gaggaaacca
    17761 ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc gggttaaaaa
    17821 tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc agcgatatcg
    17881 cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac agcgtgatga
    17941 tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt ttatttgttg
    18001 ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat gatgaagagc
    18061 accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt tttgtagaga
    18121 tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa catgtagaac
    18181 atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc gggcagcgtg
    18241 acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc gtacagcgat
    18301 ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa gtagcgccag
    18361 atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca cagcggtgcc
    18421 tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta gccgttgttg
    18481 aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga gaggatcacc
    18541 agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc cacggccagc
    18601 ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat ccacagcgag
    18661 tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac aaacgttgac
    18721 tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga caccatcggc
    18781 cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag cgcaaacggc
    18841 atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg gtttagtgct
    18901 gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg agatcggctt
    18961 tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc acatcgccca
    19021 tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc agttcaccga
    19081 ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc caggtggcgg
    19141 tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc acccagtcgg
    19201 caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg gtcttttgtc
    19261 cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc tcggttggca
    19321 ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg atataggtgg
    19381 agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc atgatgtcgt
    19441 tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc tgcatcatct
    19501 ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag ttggataacg
    19561 caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc gcaataccgt
    19621 attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt tgccagtttt
    19681 tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg ccgccaatcc
    19741 aggcgctgac aggaacacca gtccatgcgc taccatcttc ggtacgcaca atacgcagta
    19801 cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc gctttgcgat
    19861 cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg atcacttccg
    19921 gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct tcttccacgg
    19981 gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc aatttgctga
    20041 taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat acatgggttt
    20101 tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat aacagcacga
    20161 tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg ccaggggcg
//
LOCUS       DAJTYM010000074        20177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTYM010000074 DAJTYM010000000
VERSION     DAJTYM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(40..2109)
                     /gene="glyS"
                     /locus_tag="OO575_002722"
     CDS             complement(40..2109)
                     /gene="glyS"
                     /locus_tag="OO575_002722"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6665628.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2119..3030)
                     /gene="glyQ"
                     /locus_tag="OO575_002723"
     CDS             complement(2119..3030)
                     /gene="glyQ"
                     /locus_tag="OO575_002723"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6665629.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3125..3424)
                     /locus_tag="OO575_002724"
     CDS             complement(3125..3424)
                     /locus_tag="OO575_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6665630.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3599..4594
                     /gene="wecH"
                     /locus_tag="OO575_002725"
     CDS             3599..4594
                     /gene="wecH"
                     /locus_tag="OO575_002725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6665631.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4636..5073)
                     /locus_tag="OO575_002726"
     CDS             complement(4636..5073)
                     /locus_tag="OO575_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6665632.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5119..5460)
                     /locus_tag="OO575_002727"
     CDS             complement(5119..5460)
                     /locus_tag="OO575_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665633.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5629..7083)
                     /gene="xylB"
                     /locus_tag="OO575_002728"
     CDS             complement(5629..7083)
                     /gene="xylB"
                     /locus_tag="OO575_002728"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6665634.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7155..8477)
                     /gene="xylA"
                     /locus_tag="OO575_002729"
                     /pseudo
     CDS             complement(7155..8477)
                     /gene="xylA"
                     /locus_tag="OO575_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8843..9835
                     /gene="xylF"
                     /locus_tag="OO575_002730"
     CDS             8843..9835
                     /gene="xylF"
                     /locus_tag="OO575_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6665635.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9913..11454
                     /gene="xylG"
                     /locus_tag="OO575_002731"
     CDS             9913..11454
                     /gene="xylG"
                     /locus_tag="OO575_002731"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6665636.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11432..12613
                     /gene="xylH"
                     /locus_tag="OO575_002732"
     CDS             11432..12613
                     /gene="xylH"
                     /locus_tag="OO575_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6665637.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12691..13221
                     /locus_tag="OO575_002733"
     CDS             12691..13221
                     /locus_tag="OO575_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6665638.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13333..13869
                     /locus_tag="OO575_002734"
     CDS             13333..13869
                     /locus_tag="OO575_002734"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6665639.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13977..14801)
                     /locus_tag="OO575_002735"
     CDS             complement(13977..14801)
                     /locus_tag="OO575_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6665640.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15121..17151
                     /gene="malS"
                     /locus_tag="OO575_002736"
     CDS             15121..17151
                     /gene="malS"
                     /locus_tag="OO575_002736"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6665641.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLCVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17329..18582
                     /gene="avtA"
                     /locus_tag="OO575_002737"
     CDS             17329..18582
                     /gene="avtA"
                     /locus_tag="OO575_002737"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6665642.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18733..19206)
                     /locus_tag="OO575_002738"
     CDS             complement(18733..19206)
                     /locus_tag="OO575_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6665643.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19308..20156)
                     /gene="yiaJ"
                     /locus_tag="OO575_002739"
     CDS             complement(19308..20156)
                     /gene="yiaJ"
                     /locus_tag="OO575_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6665644.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
BASE COUNT         5071 a         5146 c         4944 g         5016 t
ORIGIN      
        1 aaagcctgcc agatggcagg ctttttaata acggcgttat tactgcaaca gcgaaatatc
       61 cgcaacgcgc aggaacagtt cgcgcagttt ctccagcatg gtcagacggt tgagacgcaa
      121 ttctttgtca tcaaccatga ccatcacttt atcgaagaaa gcatcaaccg gctcacgcag
      181 ctctgccagt tcgaccagcg catcctggta acgaccttcc gcaaagtacg gctccagctt
      241 gtcacgtagc accacaacct gcatcgccag tttaatttct tccggctctt tcagggtaga
      301 ggcattcacg cgatcgctca gcacttcgtc agatttcgcc aggatgttag atacacgttt
      361 gttcgccgcc gccagtgctg cagctgcttc cagggtacgg aagtgcgata ccgctttcat
      421 gcgggcatcg aaatcagccg gacgagtcgg acgacgcgcc agtaccgcct ggatggtgtc
      481 aacggtataa ccttcgtcct gataccaggc gcggaagcga ccgagcataa agtcgataac
      541 atcatcaact acgttggcat tagtcagctt atcgccatac agacgcaccg cttcttcggt
      601 cagcgtttgc agatcaaggt tgaggttctt ctcaacgata attcgcagca cgccaagtgc
      661 ggcacgacgc agcgcaaacg ggtctttgtc gcctttcgga tgctgaccga taccgaagat
      721 acccgccaga gtatccatct tatcagcaat cgccagcgca caagctaccg ggttggacgg
      781 caggtcatca ccggcaaagc gcggctgata ctgctcattc agcgcgacgg ctacgtcttc
      841 cgcttcgcca tcgtgacgcg cgtagtgcat ccccataacg ccttgggtgt cggtgaactc
      901 gaagaccatg ttggtcatca ggtcacactt agacagcagg cccgcacggg ttgcgtggtt
      961 aacgtcagcg ccaatctgtt cggcgatcca gccagccagc gcctggatgc ggtcagtctt
     1021 gtcacgcagc gtacccagct gttgctggaa cagcacggtt tgcaggcgcg gcaggttatc
     1081 ttccagacgt tttttgcggt cggtgttgaa gaagaactcg gcatccgcca gacgtggacg
     1141 aacgactttc tcgttaccag agataatttg ctgcggatct ttcgattcga tgttggcaac
     1201 gaagataaag ttcggcagca gtttgccgtc gttcgcatac accgggaagt acttctggtc
     1261 acctttcatg gtgtaaacca gggcttcagc cggcaccgcg aggaatttct cttcgaattt
     1321 tgcggtcaga acgaccggcc actccaccag cgaagccact tcttccagca ggctttcgct
     1381 taagtcagcg ttaccgccaa tcttacgcgc tgcttcttcg gcatcggctt taatcttcgc
     1441 tttacgttct tcgtaatcgg cgatgacttt cccgcgctca cgcagaattt ccggatactg
     1501 atcggcgttg tcgatggtga attccggttc gcccataaag cggtggccgc gaatcacgcg
     1561 atcggactga atgcccagaa tggttgccgg aatgactttg tcgcccagca gcagggtcac
     1621 ggtgtgcacc ggacgcacga agtgcacgtc gcttgcaccc cagcgcatca gtttcgggat
     1681 cggcagtttc gccagcgaag tcgcaaccat attcggcagc agtgcttcgg tgctttcgcc
     1741 cttcacatgg gcgcgataca gcagccattc gcctttatcg gtagtcagac gctcagcctg
     1801 gtcaacggta ataccacaac cacgcgccca accttctgcc gctttgctcg gttttccttc
     1861 agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg atttcacgat ctggttgtgc
     1921 ttccgccagg ttagctactt tcagcgccag acgacgcgga gcagcaaacc attgaacggt
     1981 gccgtgtgcg aggcctgcgt tatccagctc cgcagtaaag ttcgcagcaa aggactctgc
     2041 caggctgcgc agtgcttttg gtggcagctc ttcagtgccg atttccacca gaaaagtttt
     2101 ctcagacata gccgcctctt acttatcttt gttgcacatc gggaagccga gggcttcacg
     2161 ggaagcgtaa tatgcttctg ccactgcttt ggtcagggtg cgaatgcgca gaatgtagcg
     2221 ctgacgctcg gtgacggaga tggctttacg cgcatccagc aggttgaagc tgtgggcggc
     2281 tttcagaata cgctcgtagg ctggcagcgg cagcggattt tccagcgcca gcagctgctg
     2341 cgcttctttc tcgtactgct caaagcaggt gaacaggaag tccacatccg cgtattcgaa
     2401 gttgtaagtg gactgctcca cttcgttctg atggaacacg tcgccgtagg tggttttacc
     2461 cagcgggccg tcgctccaga ctaggtcgta aacgctgtct acgccctgaa tgtacatggc
     2521 cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt ttacactcca gaccaccaac
     2581 ctgctggaag taagtgaact gcgtcacttc catgccgttt agccacactt cccagcccag
     2641 tccccaggcc cccagagtcg ggttttccca gttatcttcc acgaaacgga tgtcgtgaat
     2701 agtcgggtcc atgcccagct ctttcagaga accgaggtac agctcctgaa tattgtccgg
     2761 cgatggctta atgaccacct ggaactgata gtagtgctgt aaacggttgg ggttttcgcc
     2821 gtagcgacca tcggtcgggc gacgagaagg ctgaacataa gcagccgcca tcggttctgg
     2881 ccccagcgcg cgcagacagg tcattgggtg agaggttccc gcgccgactt ccatgtccaa
     2941 tggttgaaca atggtgcagc cctggcgagc ccagtaatcc tgtaaggtca ggatcaagcc
     3001 ctggaaggtc ctggtatcaa acttttgcat attatttcgt gctggatacg tgtggattta
     3061 aaggaaggga tcagtatacc cgctggatgg aagatataca gtacgaaacg ggaaaaagca
     3121 gggcttaacg catggaaaga tgcaaaaaat ggccatctgc gtcaaaagaa cagacaaact
     3181 gctctttacg gaaggtataa ccgcgcattt catagcttcc ctggaactgt tcgaaggcgg
     3241 tgacgtcgat tttttgctcg ccggtgttat aacgttttgc cgcctgatcc ttgcataacg
     3301 cttccatatt caacgaacgc agaggatcaa cccttacccg ttgtgctttc tgcacgggtg
     3361 acggtgtaga acaacccact agcaccataa gcgccattgc cggaaagaaa gcgttcatca
     3421 tcattaaatt accgtctggt ttgcaggtga gtcttattat ttatatcggt atagtggctg
     3481 atatcttacg aatttcctta agcctctata ggctgtagtg ataaaactca gattattcca
     3541 gtgcgccaac caaatgattt atcggtcaga ctttttttaa ccagatacag agacacagat
     3601 gcagcccaaa atttactgga ttgataacct gcgagggata gcgtgtttaa tggtggtgat
     3661 gattcacacc actacctggt atgtgaccaa tgctcatagt gttagccccg tcacatggga
     3721 tatcgccaat gttctgaatt ctgcctctcg tgtcagcgtg ccgctatttt tcatgatttc
     3781 cggctatctc ttttttggcg aacgcagcgc ccagccgcgc catttcttgc gtatcggctt
     3841 atgtctgatt ttttatagcg caatcgcact gctctacatt gcgctgttta cctccatcaa
     3901 tatggagtta gcgctgaaaa acctgctgca aaagccagtg ttttaccact tgtggttttt
     3961 cttcgcgatt gcggtgattt atctggtttc accgctgatt caggtgaaga acgtcggcgg
     4021 aaaaatgttg ctggtgctaa tggtggtgat tggcattatc gctaacccaa acacagtgcc
     4081 gcagaaaatt gacggttttg aatggctgcc aattaactta tatatcaatg gcgatacttt
     4141 ttactacatt ctgtatggca tgttgggccg cgctataggg atgatggaca cacagcataa
     4201 agcactgtcg tgggtgagcg ccgcgctgtt tgcgacgggg gtttttatta tctctcgcgg
     4261 gacattatat gaattgcagt ggcgcggaaa ttttgccgat acctggtatc tttactgtgg
     4321 gccgatggtt tttatctgcg caatcgcgct attgactctg gttaaaaaca cgctggatac
     4381 gcgtaccatt cgcggacttg gcttaatctc ccgccattca ttgggtatat acggattcca
     4441 cgccttgatt atccatgcgc tgcgcacccg gggaattgag cttaaaaatt ggccaatact
     4501 ggatattatt tggatctttt gcgcgacgtt ggcagcgagt ttgttacttt ctatgctggt
     4561 acaacgaatc gacagaaaca gattagtgag ttaagtaaaa gcccgttcac attggactga
     4621 ccgggcttac gtgaattatt cgctgtattc ttcctgggta acctgtgttt cttttggcgg
     4681 gtttattccg gcatcacgaa tattcttctg caccgctact gcaccaaaca agcttaagaa
     4741 gaaagccagt ccataaaaac ctttttcact gagtaataat gtcgcattcc acagacctac
     4801 catcagtaga gcaacagaga taataaagac agtcaggcag gtcatataat aaatggaagt
     4861 ggtcggtatg ccttcatact tgtcccgaac ggtcttttga taagacgcag cagaaaacag
     4921 tcctaacacc agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca
     4981 tagccctaac aggtaggtaa cgataccacc aacgagagct atccatgaca caatactaaa
     5041 ggccggtgaa taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt
     5101 gtaagagctt tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac
     5161 aaaaaaggcg atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc
     5221 gataaacaag agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt
     5281 atccagttgc ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat
     5341 taacacgcca agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac
     5401 cagataaacc agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat
     5461 aacgatctcc atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaaa
     5521 aatgccatta acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag
     5581 atatatagat gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa
     5641 tggcagaagt tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata
     5701 acgctgcgca tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag
     5761 cgatttctct ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac
     5821 atcccccccc gtacggtaat cgagctgctg accgctgata tccgccagca tctgacgcca
     5881 gtactcacta cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc
     5941 atgcacgaca tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc
     6001 cagttcattg gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt
     6061 attgtgtggt gtacgctcgc cggaaagata aggcagaaac caaactggct cggcactttc
     6121 atcagcctgt tgagctgcag cgattaaagc tgggacattg cacaggccgg ttaatttcgc
     6181 ggcccaatcc agacacgatg ctgcactcag cattacagac attaaatgcc aacgctgcgg
     6241 tagcgcatgg caaaagctgt gtacagcgct ttctggcttg cttaagaacc cttcgctgac
     6301 agcaaaatag acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc
     6361 cacaccaact gcaccagctg cattgtcgcc accgcctgcg acaactggca ccgtcgccat
     6421 accccacgct ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa
     6481 tgcgggcatc tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg
     6541 ctttgcgaca tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc
     6601 cgtcatacgc agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat
     6661 ctccggctca tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag
     6721 gttgccggta atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc
     6781 acagcgcccg tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa
     6841 ggttgctcca tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg
     6901 atcgcccaga gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc
     6961 cgaccaaagt ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaag caaccacctc
     7021 accctgctcg ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata
     7081 catatcgatc gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac
     7141 tgcacagtta gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc
     7201 tggcgaccac tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct
     7261 gccagtgaca tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa
     7321 cgctgcgcga tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc
     7381 gccagtgcca tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta
     7441 ctttgacgac gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg
     7501 agaatttcat acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc
     7561 cagcccagtt gcgcatcgcc acggttggcg tcgacagaac cgaacaggcc aagcgcaatg
     7621 gcggtggcta tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg
     7681 ttcagtttaa tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca
     7741 tcgtaatcat attgatgttt agtcggttct tgcggtttcg gttcgataag caacgtgccc
     7801 tagaagccga ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca
     7861 cgctcctgac gcaagtcggt atttaacagc gtttcgtaac cttcacgacc accccacagg
     7921 acatagtttt caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc
     7981 gcccagctga agacttcagg atctgggttc gtcgccgcac ccgcgccata gcgagggttt
     8041 gtaaagcagt tagcggttcc ccacagcagc ttcacgccgc tctcttcttg cttggctgcc
     8101 aggacatcaa ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcaggg
     8161 gaaacatcca catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat
     8221 gcgacatctg ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga
     8281 ttaaacgccc ccacaccaaa catgtccgcc ccgttccagc agaaggtgtg ccagtagcag
     8341 gcggcaaaac gcaagtgctc ttccatacgc ttacccaaca ccagttcgtc gggattgtag
     8401 tgacggaatg ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg
     8461 tcaaaatagg cttgcatatt gaactccata atcaggtaat gccgcgggtg atggatgatg
     8521 tcgtaatatt gggcactccc tttcaattgc tcaattatgt tatttcacac tgctattgag
     8581 ataattcaca agtgtgcgct cgctcgcaaa ataaaatggg atgatgaaaa tgggtaattc
     8641 ctcgaagata aaaatgcaat aagtacaatt gcgcaacaaa agtaagatct cggtcataaa
     8701 tcaagaaata aaccaaaaat cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt
     8761 ttagttgcta aaaattggtt acgtttatcg cggtgattgt tacttattaa agctgtcctc
     8821 taactacaga aggccctaca ccatgaaaat aaagaacatt ctactcaccc tttgcacctc
     8881 acttctgctt accaacgttg cggcacacgc caaagaagtc aaaataggta tggcgattga
     8941 tgatctccgt cttgaacgct ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc
     9001 tctcggtgcg aaagtatttg tacagtctgc aaatggcaat gaagaaacac aaatgtcgca
     9061 gattgaaaac atgattaacc ggggtgtcga tgttcttgtc attattccgt ataacggtca
     9121 ggtattaagt aacgttgtaa aaaaagccaa acaagaaggt attaaagtat tagcttacga
     9181 ccgtatgatt aacgatgcgg atatcgattt ttatatttct ttcgataacg aaaaagtcgg
     9241 tgaactgcag gcaaaagcct tggtcgatat tgttccgcaa ggtaattact tcctgatggg
     9301 cggctcgccg gtagataaca acgccaagct gttccgcgcc ggacaaatga aagtgttaaa
     9361 accttacgtt gattctggaa aaattaaagt cgttggtgac caatgggttg atggctggtt
     9421 accggaaaac gcattgaaaa ttatggaaaa cgcgctaacc gccaataata acaaaattga
     9481 tgctgtagtt gcctcaaacg atgccaccgc aggtggggca attcaggcat taagcgcgca
     9541 aggtttatca gggaaagtag caatttccgg ccaggatgcg gatctcgcag gtattaaacg
     9601 tattgctgcc ggtacgcaaa ctatgacggt gtataaacct attacattgc tggcaaatac
     9661 tgccgcagaa attgccgttg aattgggcaa tggtcaggag ccaaaagcag atacctcact
     9721 gaataatggc ctgaaagatg tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa
     9781 caacatcaaa gatacggtaa ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt
     9841 acgccccggc gcggagcggg ggcgtgattt ctctccatgc cgcgtgaatg aattggctta
     9901 ggtggagtcg ttatgcctta tctacttgaa atgaagaaca ttaccaaaac cttcggcagt
     9961 gtgaaggcga ttgataacgt cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt
    10021 ggggaaaatg ggtctggtaa atcaacgctg atgaaagtgc tgtgtggtat ttatccccat
    10081 ggctcctacg aaggcgaaat tatttttgcg ggagaagaga ttcaggcgag tcacatccgc
    10141 gataccgaac gcaaaggtat cgccatcatt caccaggaat tggccctggt gaaagaattg
    10201 accgtgctgg aaaatatctt cctgggtaac gaaataaccc acaatggcat tatggattat
    10261 gacctgatga cgctacgcta tcagaagctg ctcgcacagg tcagtttatc catttcacct
    10321 gatacccgcg ttggcgattt agggcttggg caacaacaac tggttgaaat tgccaaggca
    10381 cttaataaac aggtgcgctt gttaattctc gatgaaccga cagcctcatt aactgagcag
    10441 gaaacgtcgg ttttactgga tattattcgc gatctacaac agcacggtat cgcctgtatt
    10501 tatatttcgc acaaactcaa cgaagtcaaa gcgatttccg atacgatttg cgttattcgc
    10561 gacggacagc acattggtac gcgtgatgct gccggaatga gtgaagacga tattatcacc
    10621 atgatggtcg agcgagagtt aaccgcgatt taccctaatg aaccacatac caccggagat
    10681 gaaatattac gtattgaaca tctgacggca tggcatccgg tcaatcgtca tattaaacga
    10741 gttaatgatg tctcgttttc cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt
    10801 ggtgccggac gtaccgagac cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa
    10861 ggaaaaattt atattgatgg caaacaggta gatattcgta actgtcagca agccatcacc
    10921 caggggattg cgatggtacc cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg
    10981 gttggtaaaa atattaccct cgccgcactc aataaattta ccggtggcat tagccagctt
    11041 gatgacgcgg cagagcaaaa atgtattctg gaatcaattc agcaactcaa agttaaaacg
    11101 tcgtcccccg accttgctat tggacgtttg agcggcggca atcagcaaaa agcgatcctc
    11161 gcccgctgtc tgttacttaa cccgcgcatc ctcattcttg atgaacctac caggggtatt
    11221 gatattggcg cgaaatatga gatctacaaa ttaattaacc aactcgtcca gcagggtatt
    11281 gccgttattg tcatctcttc cgaattacct gaagtgctcg gccttagcga tcgtgtactg
    11341 gtgatgcatg aagggaaact aaaagccaac ctgataaatc ataacctgac tcaggagcag
    11401 gtgatggaag ccgcattgag gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa
    11461 attggccgta ccgacatccg gtggcttttc tgggctgaaa tcactgaatt tgcaggtctt
    11521 cgtgatgatt gcagctatca tcgcaatcat gctgttcttt acctggacca ccgatggtgc
    11581 ctacttaagc gcccgtaacg tctccaacct gttacgccag accgcgatta ccggcatcct
    11641 cgcggtagga atggtgttcg tcataatttc tgctgaaatc gacctttccg tcggctcaat
    11701 gatggggcta ttaggtggcg tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc
    11761 acttaccatc attgtgacgt tggttctggg actgcttctc ggtgcctgga acggatggtg
    11821 ggtcgcgtac cgcaaagtcc cttcatttat tgtcaccctc gcgggcatgt tggcatttcg
    11881 cggcatactc attggcatca ccaacggcac gactgtatcc cccaccagcg ccgcgatgtc
    11941 acaaattggg caaagctatc tccccgccag taccggcttc atcattggcg cgcttggctt
    12001 aatggctttt gttggttggc aatggcgcgg aagaatgcgc cgtcaggctt tgggtttaca
    12061 gtctccggcc tctaccgcag tagtcggtcg ccaagcttta accgctatca tcgtattagg
    12121 cgcaatctgg ctgttgaatg attaccgtgg cgttcccact cctgttctgc tgctgacgtt
    12181 gctgttactc ggcggaatgt ttatggcaac gcggacggca tttggacgac gcatttatgc
    12241 catcggcggc aatctggaag cagcacgtct ctccgggatt aacgttgaac gcaccaaact
    12301 tgccgtgttc gcgattaacg gattaatggt agccatcgcc ggattaatcc ttagttctcg
    12361 acttggcgct ggttcacctt ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg
    12421 cgtgattggc ggcaccagcc tggctggcgg tgtgggaagc gttgccggag cagtaatggg
    12481 ggcatttatc atggcttcac tggataacgg catgagtatg atggatgtac cgaccttctg
    12541 gcagtatatc gttaaaggtg cgattctgtt gctggcagta tggatggact ccgcaaccaa
    12601 acgccgttct tgattttgat aaaaattttc tcaaagccgg ttacgtatta ccggttttga
    12661 gtttttgcat gattcagcag gaaaagaacc atgtttacta aacgtcaccg catcacatta
    12721 ctgttcaatg ccaataaagc ctatgaccgg caggtagtag aaggcgtagg ggaatattta
    12781 caggcatcac aatcagaatg ggatattttc attgaagaag atttccgcgc ccgcattgat
    12841 aaaatcaagg actggttagg agatgtcgtc attgccgact tcgacgacaa acagatcgag
    12901 caagcgctgg ctgatgtcga cgtccccatt gttggggttg gcggctcgta tcaccttgca
    12961 gaaagttacc cacccgttca ttacattgcc accgataact atgcgctggt tgaaagcgca
    13021 tttttgcatt taaaagagaa aggcgttaac cgctttgctt tttatggtct tccggaatca
    13081 agcggcaaac gttgggccac tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa
    13141 aagtatcgcg gagtggttta tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg
    13201 caaaatcggc tggcagactg actacaaacg ctgccaccgc aaaccgggat tattgccgtt
    13261 actgacgccc gagcgcggca tattctgcaa gtatgtgaac atctacatat tcccgtaccg
    13321 gaaaaattat gcgtgattgg catcgataac gaagaactga cccgctatct gtcgcgtgtc
    13381 gccctttctt cggtcgctca gggcgcgcgg caaatgggct atcaggcggc aaaactgttg
    13441 catcgattat tagataaaga agaaatgccg ctacagcgaa ttttggtccc accagttcgc
    13501 gtcattgaac ggcgctcaac agattatcgc tcgctgaccg atcccgccgt tattcaggcc
    13561 atgcattaca ttcgtaatca cgcctgtaaa gggattaaag tggatcaggt actggatgcg
    13621 gtcgggatct cgcgctccaa tcttgagaag cgttttaaag aagaggtggg tgaaaccatc
    13681 catgcaatga ttcatgccga gaagctggag aaagcgcgca gtctgctgat ttcaaccacc
    13741 ttgtcgatca atgagatatc gcaaatgtgc ggttatccat cgctgcaata tttctactct
    13801 gtttttaaaa aagcatatga cacaacgcca aaagagtatc gcgatgtaaa tagcgaggtc
    13861 atgttgtagc gatgatgaga attgtcggcg tcacatcagg gaactcagca cagccacctg
    13921 atgcgagcaa aagccttatc cgaccgacaa atcagataag gctcgaggaa atgaaattac
    13981 atatgcgccg cgattaaccg ttggttatcc tggtacattg cgaacaggta gttgttataa
    14041 ctcttccctt tggtcgagta gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc
    14101 tgatccgctt tacgcagctg tgcacgcgat ttacggaacg aagagtaagc cgggtgcgta
    14161 ttcaggttag tgacataggc gctcaccgat tctttgacag aactaaactg tgagtaccct
    14221 ttcactttac ccggcgcatt ggtacaacgt cctttcatgc atttcatgcc gaacagattg
    14281 ttattgttgc gcgccagctt cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc
    14341 accatactgg tagggataat gtctacgcgt tcaagcaagg tattccacgg gatttttcgc
    14401 gtattaccag accacttcac cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca
    14461 gcaggtgccc attggccctg atactgtttt gaaatcagcc agttacgttc tgcagtaatg
    14521 gctgcatttt ggctggtaat gtaaggcatt acggtccgga gaaacgcctt tttccttggt
    14581 gttccggaag ggtattttcg caaatcagga agtgaactac tctttgcact attgcgagaa
    14641 tactcttgtt tactgcttac ctgtttatta ctagctttag ttaagtggga cttttgactc
    14701 gctgttgttg tgtgcgtctt cgctaacacc tcactcgaaa acaccagagt gagtaacata
    14761 agaatcatcg ccccatatcg tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt
    14821 taatcattcc aacaccttat atttttcaca aatttgagag ttgaatctca aatcatatca
    14881 aaaatagctg tcaagagcac cccaaggaat agtccaaatc tgaaactatg tcacgagtta
    14941 acgattcaga ttggcgctaa atcgcagaaa atgtgggggt tatcgcaaaa ttcagccgtt
    15001 ttttgcgcgc gatcgctcac ccttgcttct catcctgtgg acttaccgct cagggatgag
    15061 ttttgtctgg cttatcgctg gcaaactgtc tgaaatcgca gcaataagga ctcatccacc
    15121 atgaaactcg ccgcctgttt tctgacactc cttcctggct tcgccgttgc cgccagctgg
    15181 acttctccgg ggttccctgc ctttagcgaa cagggaacgg gaacatttgt cagccacgcg
    15241 cagttgccca aaggtacgcg tccactcacg ctaaattttg accaacagtg ctggcagcct
    15301 gcagatgcga taaaactcaa tcagatgctt tccctgcaac cttgtagcaa cacgccgcct
    15361 caatggcgat tgttcaggga cggcaaatat acgctgcaaa tagacacccg ctccggtacg
    15421 ccaacattga tgatttccat ccagaacgcc gccgaaccgg tagcaaacct ggtccgtgaa
    15481 tgcccgaaat gggatggatt accgctcacg ctggatgtca gcgccacttt cccggaagga
    15541 gccgccgtac gggattatta cagccaacaa attgcgatag tgaagaacgg tcaaataacg
    15601 ttacaacccg ctgctaccag caacggttta ctcctgctgg aacgggcaga aactgacaca
    15661 tccgcccctt tcgactggca taacgccacg gtttactttg tgctgacaga tcgtttcgaa
    15721 aacggcgatc ccagtaatga ccagagttac ggacgtcata aagacggtat ggcggaaatt
    15781 ggcacttttc acggcggcga tttacgcggc ctgaccaaca aactggatta cctccagcag
    15841 ttgtgcgtta atgctttatg gataagcgcc ccatttgagc aaattcacgg ctgggtcggc
    15901 ggtggtacaa aaggcgattt cccgcattat gcctaccacg gttattacac acaggactgg
    15961 acgaatcttg atgccaatat gggcaacgaa gccgatctac ggacgctggt tgatagcgca
    16021 catcagcgcg gtattcgtat tctctttgat gtcgtgatga accacaccag ctatgccacg
    16081 ctggcggata tgcaggagta tcagtttggc gcgttatatc tttctggtga cgaagtgaaa
    16141 aaaacgctgg gtgaacgctg gagcgactgg aaacctgccg ccggacaaac ctggcatagc
    16201 tttaacgatt acattaattt cagcgacaaa acaggctggg ataaatggtg gggaaaaaac
    16261 tggatcagaa cggatatcgg cgattacgac aatcctggat tcgatgatct cactatgtcg
    16321 ctggcctttt tgccggatat caaaaccgaa tcaactaccg cttctggtct gccggtgttc
    16381 tataaaaaca aaacggatac tcacgctaaa gtcatcgaag gctttacacc tcgcgattac
    16441 ttaacccact ggttaagtca gtgggtccgc gactatggga ttgatggttt tcgggtcgat
    16501 accgccaaac atgttgagtt gcccgcctgg cagcaactga aaaccgaagc cagctccgcg
    16561 cttcgcgaat ggaaaaaaac taaccccgac aaagcattag atgacaaacc tttctggatg
    16621 accggtgaag cctggggcca cggcgtgatg caaagtgact actatcgcca cggcttcgat
    16681 gcgatgatca atttcgatta tcaggagcag gcggcgaaag cagtcgattg tctggcgcag
    16741 atggatacga cctggcagca aatggcggag aaattgcagg gtttcaacgt gttgagctac
    16801 ctctcgtcgc atgatacccg tctgttccgt gaagggggcg acaaagcagc agagttatta
    16861 ctattagcgc caggcgcggt acaaatcttt tatggcgatg aatcctcgcg tccgttcggt
    16921 cctacaggtt ctgatccgct gcaaggtaca cgttcggata tgaactggca ggatgttagc
    16981 ggtaaatctg ccgccaacgt cgcgcactgg cagaaaatca gccagttccg cgcccgccat
    17041 cccgcaattg gcgcgggcaa acaaacgaca ctttcgctga agcagggcta cggctttgtt
    17101 cgtgagcatg gcgacgataa agtgctggtc atctgggctg ggcaacagtg acttttccgg
    17161 cttcccgttc gtcagtacct cgggaagccg ccaaccagga taaaatgtca gccctaatca
    17221 gcgttgcggg ataaagcacc gctcactctt caacagaccg atttgcaccc cagcaaatgt
    17281 agcgttattg ttaccttcct tgctacagag ttcgacagat atcccgctat gacattctcc
    17341 ctttttggtg acaaatttac ccgccactcc ggcattacgc tgctgatgga agatctgaac
    17401 gacggtttac gcacgcctgg cgcgattatg ctcggcggcg gtaatccggc gcagatcccg
    17461 gaaatgcagg actacttcca gacgctactg accgacatgc tggaaagtgg caaagcgact
    17521 gatgcactgt gtaactacga cggtccacag gggaaaacgg agctactcac actgcttgcc
    17581 ggaatgctgc gcgagaagtt gggttgggat atcgaaccac agaatattgc actaacaaac
    17641 ggcagccaga gcgcgttttt ctacttattt aacctgtttg ccggacgccg tgccgatggt
    17701 cgggtcaaaa aagtgctgtt cccgctggca ccggaataca ttggctacgc tgacgccgga
    17761 ctggaagaag atctgtttgt ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag
    17821 tttaaatacc acgtcgattt tgagcatctg catattggcg aagaaaccgg gatgatttgc
    17881 gtctcccggc cgacgaatcc aacaggtaat gtgattactg acgaagagtt gctgaagctt
    17941 gacgcgctgg cgaatcaaca cggcattccg ctggtgattg ataacgctta tggcgtcccg
    18001 ttcccgggta tcatcttcag tgaagggcgc ccgctatgga atccgaatat cgtactgtgc
    18061 atgagtcttt ccaagctggg gctgcctggc tcccgctgcg gcattatcat cgccaatgaa
    18121 aaaatcatca ctgccatcac caatatgaac ggcattatca gcctggcacc tggcggtatt
    18181 ggtccggcga tgatgtgtga aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca
    18241 gtcatcaaac cgttttacta ccagcgtgtt caggaaacta tcgccatcat tcgccgctat
    18301 ttaccggaaa atcgctgcct gattcataaa ccggaaggag ccattttcct ctggctatgg
    18361 tttaaggatt tgcccattac gaccgagcag ctctatcagc gcctgaaagc acgcggcgtg
    18421 ctgatggtgc cggggcacaa cttcttcccg ggactggata aaccgtggcc gcatacgcat
    18481 caatgtatgc gcatgaacta cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt
    18541 ctggcggaag agatagaaag agcctgggct gaaagtcact aaaaattgcc tgatgcgcta
    18601 cgcttatcag gcctacatga tttctgcaat atattgaatt tggaagaatt tgtaggccgg
    18661 ataaggcgtt tacgccgcat ctggcataaa caaagcgcac tattccggca ttgagaaacg
    18721 ccggaaaacg tttcaaacag gctgctgctg tagccgatgc cgctgcactt tctcgacatc
    18781 catgcactgc aacgcctttg tcgggcaggc ttcaacacag gccggccccg tctcccgatg
    18841 ccagcacaga tcgcacttaa tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc
    18901 accaaacggg caagccagca tacagctttt acagccaatg caacgtgttt gttcaacgaa
    18961 aatatgccca tgttcgcggc ttatcgcgtc aacagggcag acattcgcgc acggtgcatc
    19021 ttcacactga tgacaggcta ctggcgtggt ccagctgtgg tctttaatga cacgaatacg
    19081 ggaaataaac tcgtctggtg acaacgcagc gcaatcctga ttctcctgat gcgacactgc
    19141 gcaagccact tcacaggtac ggcaaccgat acatttcgtc gcatccgcaa taataaaccg
    19201 gttcatgatc attcctttgc actaaaacag cagagtgcca gagagcgcgt ccctactgct
    19261 ttgatccaac gaaggaaatg ttaaagtttt ggcacttgtc caaagcgtta tgtgatcgcg
    19321 cccaggtcat ccctgacagt aaatcccagt tcattagaaa tagcctgcgc ggtttcacgc
    19381 agtggtttca ggagattttt ctctcccacc tgtttcagac gtgatgtcga aagcgaaatc
    19441 gacacggcgt acggcacccg cccatgaata tcaaacaccg gaacagcaat acaggagacg
    19501 ccgagttcgt tttcttctct gtccatcgcc gctccgcttt cacgaatgtg cgccagttcg
    19561 tcgaacatcg cgggcagctc ggtaatggta ttgcgggtta acggctggat ctcatgctga
    19621 tggttttccc agtatgactt cacgtagtcc gggtgaccaa acgccatgta gatcttgccc
    19681 attgcggaac agtagagcgg catatgctgg ccaatatagg cacgggttcg cagcatcccg
    19741 gttgtgggtt ccagcttata aatcaaaata gcgtgatcgt cttcgcggct ggagaagtta
    19801 atggtttcac cagtggcgat gttcagtgcc tcaagatgcg gagcggcgat atgaatgata
    19861 ttcagcgaag acagcgcctt ctgcccgacg gcaataaatt tggtagtcag gcggtaactc
    19921 cctgcggcgg gcgcggtggt cacatagccg caggactgta atccctgcaa taagcgatgg
    19981 acggtactct tatttaaacc agccagctcc gaaagatgcg ccaacggaca accgtttgga
    20041 tagttgctca aaatctcaat cagcatcaac ccgcgaaaca gactctggct tccggctgga
    20101 cgctcttttt cctgcgccat ctcgttctct ttttttccca tcacttcttt ccccattttg
    20161 tcgcgtcctg atggtag
//
LOCUS       DAJTYM010000075        19967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTYM010000075 DAJTYM010000000
VERSION     DAJTYM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(23..781)
                     /gene="aroD"
                     /locus_tag="OO575_002740"
     CDS             complement(23..781)
                     /gene="aroD"
                     /locus_tag="OO575_002740"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6665645.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(812..1678)
                     /gene="ydiB"
                     /locus_tag="OO575_002741"
     CDS             complement(812..1678)
                     /gene="ydiB"
                     /locus_tag="OO575_002741"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6665646.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1690..2962)
                     /locus_tag="OO575_002742"
                     /pseudo
     CDS             complement(1690..2962)
                     /locus_tag="OO575_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3145..4350)
                     /locus_tag="OO575_002743"
     CDS             complement(3145..4350)
                     /locus_tag="OO575_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6665647.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4450..4806)
                     /locus_tag="OO575_002744"
     CDS             complement(4450..4806)
                     /locus_tag="OO575_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6665648.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4942..5049)
                     /gene="rprA"
                     /locus_tag="OO575_002745"
     ncRNA           complement(4942..5049)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO575_002745"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5235..6347)
                     /gene="ydiK"
                     /locus_tag="OO575_002746"
     CDS             complement(5235..6347)
                     /gene="ydiK"
                     /locus_tag="OO575_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6665649.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6736..9792
                     /locus_tag="OO575_002747"
     CDS             6736..9792
                     /locus_tag="OO575_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6665650.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9789..10199
                     /gene="menI"
                     /locus_tag="OO575_002748"
     CDS             9789..10199
                     /gene="menI"
                     /locus_tag="OO575_002748"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6665651.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10299..10487
                     /locus_tag="OO575_002749"
     CDS             10299..10487
                     /locus_tag="OO575_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6665652.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11035..11403
                     /gene="sufA"
                     /locus_tag="OO575_002750"
     CDS             11035..11403
                     /gene="sufA"
                     /locus_tag="OO575_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6665653.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11412..12899
                     /gene="sufB"
                     /locus_tag="OO575_002751"
     CDS             11412..12899
                     /gene="sufB"
                     /locus_tag="OO575_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6665654.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12909..13655
                     /gene="sufC"
                     /locus_tag="OO575_002752"
     CDS             12909..13655
                     /gene="sufC"
                     /locus_tag="OO575_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6665655.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13630..14901
                     /gene="sufD"
                     /locus_tag="OO575_002753"
     CDS             13630..14901
                     /gene="sufD"
                     /locus_tag="OO575_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6665656.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14898..16118
                     /gene="sufS"
                     /locus_tag="OO575_002754"
     CDS             14898..16118
                     /gene="sufS"
                     /locus_tag="OO575_002754"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6665657.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16131..16547
                     /gene="sufE"
                     /locus_tag="OO575_002755"
     CDS             16131..16547
                     /gene="sufE"
                     /locus_tag="OO575_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6665658.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16696..17700
                     /gene="ldtE"
                     /locus_tag="OO575_002756"
     CDS             16696..17700
                     /gene="ldtE"
                     /locus_tag="OO575_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6665659.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17763..17999)
                     /gene="lpp"
                     /locus_tag="OO575_002757"
     CDS             complement(17763..17999)
                     /gene="lpp"
                     /locus_tag="OO575_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6665660.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18310..19722)
                     /gene="pykF"
                     /locus_tag="OO575_002758"
     CDS             complement(18310..19722)
                     /gene="pykF"
                     /locus_tag="OO575_002758"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6665661.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5212 a         4820 c         5111 g         4824 t
ORIGIN      
        1 atttccgccg aaatattatt gcttatgcct gatgtaaaat agttaatacc gtgcgcaaat
       61 catttaccga gatttgccct ggcgcagagg ctttttttac cgcaccaaaa gttgccgccg
      121 agccaaatac ttcaccaacc agacgagaaa ttacgccagt ttttgccatc gacatcgtga
      181 tgattggacg atcggcatac tgctcctgca tctccagggt cgcggcaagc aacgtcagca
      241 catcgctggt actttgcggc atcagcgcaa tcttaggaat atcggcgtcg aaggactgca
      301 ttttgcgcag acgggcaatg atttcttcgg cttccggcgt tttatggaag tcatggttgg
      361 acatgacaac tttcacatca tgcgcgtggg cgtaggcgac ggtttctttg acctgatcat
      421 cgccggtaaa taactccaga tcgatcatat caaccaggcc gctgtcgatg gctgcacgat
      481 tgagagcaat ataagcctcg gtggaaatcg cctgctcgcc gccttctttg gcactgcgga
      541 aggtaaacag cagcggtttt tctggcatgg tttcacggag aatttttgcc gccgccatga
      601 cagactccac attggagagg tcggcaaagt ggtccacacg ccattccaga atatcaaagt
      661 ccgcttcacg ataggcgaga gcttcggatt tcacgcgggc gatatctttc gccatcagcg
      721 agacgatgat tttaggtgcg cccgcaccaa tgacgagatc ttttacagtt acggttttca
      781 ttttttaccc tttctgcacg cggtcagcct gtcaggcacc gaaccccatg acctgtttaa
      841 catattccag agggaaatct ttgccagtcc ataacgtgaa ctgttcagcc ccttgccaca
      901 acaacatgcc gtatccatca atcgttttgc aaccagcttg ttgtgcctgc tgcaataact
      961 tcgtcatatg cgggttatac acgcattcag tgaccagcag tcccggatgt aacagactga
     1021 tatcattaac caatgattca ttctcaaggg gtttcatacc tacttttgtg ccattggtta
     1081 aaatgtcggc ggaagccagg gcttcagcaa aggcttgctg atcggcgaga tcggtatccg
     1141 tgacgacaca atcggtgttt tcgttaaccc gctgcgcgaa ggcgagggct ttatcgaaga
     1201 actcatcccg acggttaaag agtttaattt cttttaaacc ttcaattgcc ccctgcgcgc
     1261 caatcgccgt tgaggcacct ccggccccta acagcaccat cgttttgcct ttgatatcaa
     1321 aaccgctctc tttaatggcg cgaatatggc ccgtgccgtc ggtgttatag ccacgcagat
     1381 agccatcatc attaacgatg gtgttgatgg cacccaccag tttggctgct ggcgttaatt
     1441 catcaacata ttcacacgcc agttgtttgt tcggcatcga cacaccagtt ccgcgcattt
     1501 tgagggcttt taatccttca attgctccgg gaaagctatc gttatccact tcgaaggcca
     1561 tataggtaaa tggcaatccc gctttttcta aggctttatt ctgcatttca ggcgataaac
     1621 tgtggcggat aggataagcc atcaatccaa tcaattcgta ttttgcggta acatccataa
     1681 ttaactcctt taacctctat gcttaattgt gtttaaccgg gtgctaaatt tgcgttcgcc
     1741 aaaccgcaca tcattctcag gaataataaa aacgcggtaa tagcggctaa aaacaataat
     1801 tgcggtaatc agggccagca gcgcgaaagt aaaatcgaga acaatgatat attgcaggcc
     1861 gatgttcgac agataaccgg taatcagcgg aataacaaag ttagccagtc cacccatcat
     1921 catataaata ctggtgactt tggctttgct tttggggaaa aactctgaca tgaccgaaac
     1981 accgagctgt aaaatgccgc cagctgctga gaaaccgata acaaaggcgc cggcattaca
     2041 caccagtgga gaagggtaca ggtagataat ggcagctgtt attgttgcca gcgcagagtt
     2101 aaatacatta gcccagatgg gccggaccat ttttttcagt agtgcggcaa aaataaagac
     2161 acagaccaac gagcccatac tgtaataaga gatggttttt aatgcctcag cttctgacat
     2221 accagcaaaa gccatcgcat atttgggcat ccacaccaca atcacataaa aggtcgagaa
     2281 tgcggctaca ccgaacagta ccgatgaaac accttccagc cagactaaag gttttgctgt
     2341 tcatttgcgg taattcatta gctacgctgg cgtccaccaa ctggctgggg aatttgcttt
     2401 tcaacagcat cagcgtgatc agtacaaaca gaatacccgg aataatcagc ccatagccgt
     2461 accagatatt attgagcaac atatagctca ccagcattgg gtagaacatt tgcccaaatg
     2521 acaccatcgc tttaaccagt atgaccgccg aaccagaggc tttcggaaag cattccatga
     2581 gcgcggggta gccacccgta tccagcgctg agttagcgat acctacgcac actgccagac
     2641 cgtaggcgag agttaaattc gggcaagcgg gaataccaaa gaagaatagc agatacatta
     2701 ttactgccat taatatcacc gcccgacgac caaacttatc ggagatcaca ccgaagaata
     2761 aaatactgat caatcgcccc aaaccgatac cggaaattaa ataggcaatg cccgcgttgt
     2821 cagtggaaaa cttttctgac agagatgtca tattttgggc aagcgtaata acactaatgc
     2881 cgtgcaggaa atagctgaag taaatacaaa gaacagccag gataaatggc gtgctgaaag
     2941 ataatttcgt taccgtacgt attgttctcc gacggtttac ataattacta tactgaagat
     3001 gattttccct gggtacatgg cataaagttt atgtttccag gaatattgca tgtcatagtt
     3061 tgtttttgat cgcgattttc cttagttagc gcagaaagag ttatttatcc gtaagatctt
     3121 gcatgacctg aaaagaatat tgcattaccc acccggagcg acattttcct ttagcgagtg
     3181 atgccgcgtt tttttgcggc tgcgtaagcc gataaacagt gccagtaaaa aaccgatggc
     3241 agtatcgaac cacataatat cggcaatact tctttgggac agatgagccg taatcaacgg
     3301 aatagtaaag gtcgcaatac tgcccgtact gtaatagatc cctgtagctt tacctttagc
     3361 gtaagggaaa cgttcagcca ttaacgtcag gccaatttgc acaacacctc cagcagaggt
     3421 aaaaccaatg acaaaagcaa atattatcac cacataaaat gtgggatgca ggcagacggt
     3481 aaacagagca ataaatgaga taaaggtgta cagcatcagt aatgttgttg ggcgaacggt
     3541 attacgaatg agtggagcgg taataaatac acaaagcagc gaacccacgg tgtagatact
     3601 gagtagtttg atcgacatag tgtatgacat gcctgcaaca aattgtccgt actgtgccag
     3661 ccactggcta accagataaa acgttgccat tgagatatag ccatataagg tataactggc
     3721 taaatcgata attgaacagc gatgttccgt agagctggtg gttttcttta tgacaggtaa
     3781 gcgacgaccc ggatggggtg ggaacgtaca acggtataaa aacagagcgt taataaacat
     3841 aatgcctgca gcaatcataa aggaccaacc gaaccacagt tcagcccaca ctaacaggct
     3901 aatgattagc ggtaataaaa attgtccgct ggaaacaaat gctttaatta aaatattggc
     3961 tgtcccaggt gagcgtggaa aagcttccat caaactggga taagtgcctg catcgagaaa
     4021 actgtttgcc attcccgcca gaaagccaaa aacataagcg ataatgatgt tattggtctg
     4081 caggatgtca aaaaagaagg ccatatagca gcacatcccg agcatgataa aagggcggcg
     4141 accaaagcga tcggataata atcctgcaaa aagcaagaca cttaatcgac caatgcccag
     4201 cgatgagata actatcgaga cacccgcggc attagtctgc caaagtgtct ccagcgaggc
     4261 catattcagg ctcatcaaaa tgacgcccat accatgcacc aggtaattaa aatacaaccc
     4321 aagtgcggta gggtaatagg gatttttcat aggttctact tagcaaaggt gctttaaaaa
     4381 gtgtagcgat gttgtgtcag tatccattca ttattgttgt aacaacgaca attcctttct
     4441 gtggaaaaat taacaaagcc gctccagatc gtgtgcatat aattcagcgg ctaccgattg
     4501 atagatcttc cagtcaataa aattgccatc agggtagact tgttgaaacg cggttaagtc
     4561 ggggaaaaaa cgcatggtgt tgttgccgat gcggttattt attttctcta tgatggcatg
     4621 aagatgtttt tttcgttgct gacgcatagc caacagttgc tcgactacac aatcaggaat
     4681 ggcgcatttg ccattttccc actgacgcca gctttcactg tcaccggtct gggcaatcca
     4741 ggtcgcgcat tcatcaatag tcatggcaaa aatatggcgg agtgcctgga gttcataagc
     4801 gttcatcgtg taatggctgc atgaaataaa caataaaata taaataacat tatcagcctg
     4861 ctgacggctt gaagagagtc acagtatctt gtgcaacatt attggaaaga gtgaggggcg
     4921 aggtagcgaa gcggaaaaat gttaaaaaaa agcccatcgt gggagatggg aaaagactac
     4981 acacagcaat tcgttgtttc actcagggga tttccatgct tataaatcaa tatgttgatt
     5041 tataaccgtg agctaatagt aggcatacgg actttttgcg tcgatcagat tcgtctcatt
     5101 agtttaaaaa ctgtaaagaa tttgcgacag gtactaccag ctgaatggcg gtaagcagga
     5161 aaatatgggg aagagcaagc aaatatcaat tagcggtgat taacaatcag gcggcagagc
     5221 cgcctgatga gaaattactt attcggtttc tcgatttcgc caagttcttc gagaatttct
     5281 tccggttggt ccgtcggtgg cgggacttct tccacccacg cggcaaacag acgccaggaa
     5341 acggctaaca gaaccggacc aataaacaga ccgatcatcc cgaaagcaat caaaccacca
     5401 ataacgccag agaggatcag gatcagcggt aaatcggcac ccatgcgaat taacattggg
     5461 cggatgacgt tatccagcgt gccaaccaca ccgctccaca ctaacaatac cgttccccag
     5521 gtggtatcgc cggtccagta gagccagata atcgccggaa tcagtaccgg caacgggcca
     5581 agctggacaa ggcaggagag gatcattaac accgttagca acgttgcata aggtacgccg
     5641 gatacggcga ggccgatacc gccaagcact gcctgtacta acgccgtcac caccacaccc
     5701 agcgccaccg cgcggatagc ctgtgccgcc agcagcacgg cggcatctcc gcgaacgcct
     5761 gccagacggg ttgcaaaatg gcgaatgcct tgtgccacct gttcaccgcg ccagtacagc
     5821 agggcactga agagaagcat cagcgcacaa tgcaccataa agcgcccgat atgcgccgcc
     5881 tgcccaacga accaggtggt ggtggtgcca atataagggc ggactttcgc catgatcgcc
     5941 gtacccccca tatccagcaa gttgtgccag cctgcataca gcttcgcgcc aatcaccgga
     6001 atggtattaa gccacgccag atcgggtaac gtcatgtcgc cgctggaaat ggctttaatt
     6061 agcgggccgc tgccgtcgac gatactgtta accagcaaag caatagggat gataaacacc
     6121 atcactaata acagcgtcat caccagaacg gcgagagagc ggcggccaaa catgatcttt
     6181 tgcaaacgta acaataccgg ccaggtggcg ataaccaccg taccggccca tgcaaagccg
     6241 agaataaagg gttgaacaat ccacagacat gccacaatca tgatggctaa aaacagcacc
     6301 gaaagcagaa tttgtgcgac atccctgggc tgacgaacat ttaccataac tacttttcac
     6361 cttttttgtg cgtcaaatcg tcgacgcgac gtgaacacgc gaaactcacc tgcataatga
     6421 tgagcaattt cagcggtttt taacaggccg attctgccct taattctgat gggcgtgtta
     6481 gaaaaaaatg tggtacaaca actcaggcaa cacgcaaacg atttactcgt cgtatttcaa
     6541 gttgcatgtg ctgcggctac gttcgttcac cccagtcact tactattgta agctcctgga
     6601 gttcactcgc ttgccgcctt cctgcaactc gaaatccata gagtaaataa acatcacctt
     6661 tcagtgcgct attgcagccg cctgaaagaa tagtgtatcg acctacatca cagaccgcag
     6721 gaaagggtca atataatgat tccacagatt tccctggcac ccggcgtcgt tcaactggtg
     6781 cttaattttt tgcaagagct ggagcaacaa ggttttaccg gcgatgcggc gacaagttat
     6841 gccgatcgtc tgacaatgtc gaccgacaac agtatttacc aacttctccc cgatgcggtg
     6901 gtatttccgc gttcaaccgc agatgtggcg ctgatcgccc gtcttgccgc gcaggaacgc
     6961 tattcatcgc tgatctttac cccccgcggc ggcggcaccg gcactaacgg tcaggcgctc
     7021 aaccagggga ttattgttga tatgtcccgc catatgaacc gcatcatcga aattaaccct
     7081 gaagagggct gggtgcgcgt tgaggccggg gtgataaaag accaactcaa tcagtacctg
     7141 aaaccgttcg gctacttttt tgcgccggaa ctttcgacca gcaaccgggc aacgctcggt
     7201 gggatgatca ataccgatgc atccggtcag ggatcgctgg tctatggcaa aacgtcagat
     7261 cacgtacttg gcgtacgcgc ggtgttgttg gggggcgata ttctcgatac gcaaccttta
     7321 cccgtcgagc tggcggaaac gctgggtaaa tccaatacca caatcgggcg aatttataac
     7381 acggtttatc aacgttgccg tcagcaacgc cagttaatca tcgacaactt ccccaaactt
     7441 aaccgctttc ttaccggtta cgatctgcgt catgtcttta acgatgagat gaccgagttc
     7501 gacctgacgc gcattctgac gggttcagaa gggacgctgg cctttattac cgaagcgcgg
     7561 ctggatatta cgcgcttgcc taaagtgcgc cgtctggtga atgtcaaata tgactctttt
     7621 gactctgcgc tgcgtaacgc gccgtttatg gttgaggcgc gggcgctttc ggtagagacg
     7681 gtggactcaa aagtgctgaa tctggcgcgg gaagatattg tctggcattc cgtcagcgag
     7741 ttgattaccg atatgcctga caaagagatg ctcggactga acattgtgga atttgctggt
     7801 gatgatgagg cgctgattga cgagcgagtt aatgcactct gtgtgcggct tgatgagctg
     7861 attgccagcc aacaggcagg tgtgattggc tggcaggtgt gccgcgagct ggcgggggtt
     7921 gaacgtatct atgcgatgcg caaaaaagcc gttggtctgc ttggcaatgc caaaggtgcc
     7981 gctaagccaa ttccgtttgc tgaggatacc tgcgtaccgc cggaacatct ggccgattat
     8041 attgctgaat ttcgcgcgct gctcgacagc cacggcttaa gctacggtat gttcggtcac
     8101 gtcgacgcag gtgtcttgca cgtccgtccg gcactggata tgtgcgatcc gcaacaagag
     8161 attttgatga agcaaatctc tgatgacgtg gtggcgctga ctgcgaaata cggtggtttg
     8221 ttgtggggcg agcacggcaa aggttttcgc gctgaataca gcccggcgtt tttcggtgag
     8281 gaactttttg cagaactgcg caaagtgaaa gcggcatttg acccgcataa ccgactcaac
     8341 ccagggaaga tttgcccgcc agaaggtctc gatgcgccga tgatgaaagt ggacgcggtg
     8401 aagcgcggta cattcgatcg gcagatcccc attgcggtac gccagcagtg gcgcggtgcg
     8461 atggagtgta acggcaacgg tttatgcttc aactttgatg cccgtagtcc gatgtgtccg
     8521 tcgatgaaga tcacccagaa ccggattcat tcaccgaaag ggcgcgcaac gctggtgcgt
     8581 gaatggctgc gcttgttggc ggatcgcggc gttgacccac tcaaactgga acaagaactg
     8641 cctgaatccg gcgtcagttt gcggacgtta attgcccgca cgcgcaatag ctggcatgcg
     8701 aataaaggcg aatatgactt ctcacacgaa gtcaaagagg cgatgtcggg ctgtctggcc
     8761 tgtaaagctt gttcgaccca gtgccccatc aaaattgatg tgccggagtt tcgctctcgt
     8821 tttctgcagc tctatcacac ccgttattta cgcccgcttc gcgaccacct cgttgctacg
     8881 gtcgagagct acgcaccgct gatggcacgc gcgccgaaaa cctttaactt cttcattaac
     8941 cagccgctgg tgcgcaaact ctcggaaaaa catatcggca tggttgattt gccactgctg
     9001 tcggtcccct cgctacaaca acaaatggtg gggcatcgct cggcaaacat gacgctggaa
     9061 cagcttgaag ctctcaatgc agagcagaaa gcgcgcacgg tgttggtggt gcaggacccc
     9121 tttaccagct attacgatgc gcaagtggtg gcggattttg tccgtctggt tgaaaaatta
     9181 ggtttccagc ctgtgttact gccattttcg ccaaatggca aagcccagca tatcaaaggc
     9241 tttcttaatc gttttgcgaa gacggcgaaa aagacggcgg atttcctcaa ccgtatggct
     9301 aagcttggga tgccaatggt gggcgtcgat ccggcgctgg tactttgtta tcgcgatgaa
     9361 tataaactgg cgctgggcga ggaacgtggc gagtttaacg tcttacttgc gaatgaatgg
     9421 ctggcaagcg cacttgagtc acatccagtg gctacagtca gcggtgaatc atggtatttc
     9481 tttggtcact gtaccgaggt taccgctttg ccgggcgcgc cagcacaatg ggccgcgata
     9541 tttgcccgtt ttggcgcgaa actggaaaat gtcagcgtgg gttgctgcgg catggcaggg
     9601 acttacggac atgaagcgaa aaaccatgaa aattcgctcg ggatctatga gttatcctgg
     9661 catcaggcta tgcagcgact gccgcgtaac cgctgtctgg cgaccggata ttcctgccgt
     9721 agccaggtaa aacgggttga aggcacgggg gtacgccatc ctgtgcaggc tttactggag
     9781 attattaaat gatatggaaa cggaaaatca ccctggaagc actgaatgct atgggtgaag
     9841 gaaacatggt ggggttcctg gatattcgct ttgaacatat tggtgatgac acccttgaag
     9901 cgacaatgcc agtagactcg cggacaaagc agcctttcgg gttgctgcat ggaggagcat
     9961 ccgtggtact ggccgaaagt atcggttccg ttgccggtta tttatgtacc gaaggtgagc
    10021 aaaaagtggt tggtctggaa atcaatgcta atcacgtccg ctcggcacga gaagggcggg
    10081 tgcgcggcat atgtaaaccg ttgcatctcg gttcgcgtca ccaggtctgg cagattgaaa
    10141 tcttcgatga gaaagggcgt ttgtgctgtt cgtcacgatt gacgaccgcc attttgtgac
    10201 gttgtcacca gaaaagtgtg acgcatattg cttttggtca ttatctgcac agtaaacggt
    10261 ggtatactgt gcaggtcttg tctaaaagcg aggatgctat gtctactcaa cttgatccca
    10321 cccagctggc cattgaattt ttacgccgtg accagagcaa tctttcacct gcccaatatc
    10381 tcaaacgtct gaaacaacta gaacttgaat ttgctgacct gcttactctc tcttctgctg
    10441 aattaaaaga agaaatctac ttcgcctggc gtctgggcgt gcattgatcc tgacttaatg
    10501 ccgcaagttc gccaaatcga cctggattaa cctccgtcca ggttcgatga ccttcttaat
    10561 actgcttatc taccgcctga tattgttcaa aaataatact aatcgcagtt tgtgttaaaa
    10621 cggcgggtta gctttatgag attattctta tcgccccttc aagagctaag ccactgagag
    10681 tgccggagat aagcgccgga tggggtagaa acccttaagc ctgtgtcgca cagacttaag
    10741 ggttttctta tttctgaacg ctgcactttt ttacctatct cttaaatagt cattttcaat
    10801 acaacttttt ctttttcatc acagccttaa tgctccttag ataataacca ttatctaaca
    10861 atgagatacc taattcttag cgtgcctgtt aacccacaca tcaggggcta tgcttattaa
    10921 aagcaggcaa aaagaatgtt ttacggtaaa gcccctgcgt ttgctgggtt gaactgataa
    10981 tcattatcac taacatgctg ttatacgctg aaagcgatga agtgaggtaa atagatggac
    11041 atgcattcag gaacctttaa cccacaggat ttcgcctggc aaggcttaac gctgacaccc
    11101 gcagcggcga tacacatccg cgagctggtg gcaaagcagc caggtatggt cggcgtgcgc
    11161 ttaggtgtga agcaaacggg ctgcgcgggc tttggctatg tgctcgacag tgttagcgag
    11221 ccggacaaag acgatctgct gtttgaacac gacggcgcga agctgtttgt cccgctgcaa
    11281 tcgatgccgt ttattgatgg cacggaagtc gatttcgttc gtgaaggact taatcagata
    11341 ttcaaatttc acaaccctaa agcccagaat gaatgtggct gtggcgaaag ctttagggta
    11401 taggcggtac tatgtctcgt aatactgaag caactgacga tgtcaaaacc tggaccggcg
    11461 gcccgctgaa ctataaagaa ggattcttca cccagttagc caccgatgag ctggcaaagg
    11521 ggataaacga agaggtggtg cgcgcaattt cggcgaagcg taatgagccg gagtggatgc
    11581 tggagtttcg tctaaacgcc tatcgcgcat ggctggagat ggaagaaccg cactggttga
    11641 aagcgcacta cgacaagctg aattatcagg attacagcta ctactcagca ccatcgtgcg
    11701 gtaattgtga cgacacttgc gcgtctgaac ctggcgcggt gcagcaaact ggcgcgaacg
    11761 cctttttaag taaagaggtg gaggcggcgt ttgagcagtt gggcgttccc gtgcgggaag
    11821 gcaaagaggt ggcggtggat gccattttcg actctgtttc ggttgccacc acttatcgcg
    11881 aaaaactggc ggagcaggga attattttct gttcctttgg tgaggcgatc cacgatcacc
    11941 cggaactggt gcgtaaatat ctcggcaccg tggtgccggg gaatgacaac ttctttgcag
    12001 cacttaatgc ggcggtagcc tctgacggta cgtttattta tgtgcctaaa ggcgtgcgct
    12061 gcccgatgga actttccacc tattttcgca ttaacgcgga aaaaaccggg cagtttgagc
    12121 gcacaattct ggtggccgac gaagacagct acgtcagcta cattgaaggc tgttccgctc
    12181 cggtgcgtga cagctatcag ttgcacgcgg cagtggtgga agtcatcatc cataaaaacg
    12241 ccgaggtgaa atattccacg gtacaaaact ggttccctgg cgataacaac accggcggta
    12301 ttctcaactt cgtcaccaag cgtgctttgt gcgaaggcga aaacagcaaa atgtcatgga
    12361 cgcaatcaga aaccgggtca gcgattacgt ggaaatatcc cagctgcatt ttgcgcggcg
    12421 ataactccat tggtgagttt tactcagtgg cgctgaccag cggtcatcag caagcggata
    12481 ccggcaccaa gatgatccac atcggtaaaa acaccaaatc gaccattatc tcgaaaggga
    12541 tctctgccgg acatagtcag aacagttatc gcggcttagt gaaaatcatg ccgacggcaa
    12601 ccaatgcgcg caatttcact cagtgcgact caatgctgat tggcgctaat tgtggggcgc
    12661 ataccttccc gtatgtcgaa tgtcgcaata acagcgcaca actggagcac gaggcaacga
    12721 catcacgtat tggtgaagat caactgtttt actgcctgca acgcgggatc agcgaagaag
    12781 acgccatctc gatgattgtt aacggtttct gcaaagacgt gttctcagaa ctgccgctgg
    12841 aatttgccgt tgaagcacaa aaactcctcg ccatcagtct tgaacacagc gtcggataag
    12901 gaataaacat gttaagtatt aaagatttac acgtcagcgt ggaagataaa gctatcctgc
    12961 gtggattaag cctcgacgtt cgtcccggcg aagttcacgc cattatgggg ccaaacggtt
    13021 cgggcaaaag taccttatcg gcaacgcttg ccgggcgcga agattatgaa gtgacgggcg
    13081 gcacggttga gttcaaaggc aaagatttgc ttgcactgtc gccggaagat cgtgcaggcg
    13141 aaggcatctt tatggccttc cagtatccgg tggagattcc aggcgtcagt aaccagtttt
    13201 tcctgcaaac ggcacttaat gcagtgcgca gctatcgcgg ccaggaaacg ctcgaccgct
    13261 ttgattttca ggatttgatg gaagagaaaa tcgctctcct gaagatgccg gaagatttat
    13321 taacccgttc ggtaaacgtt ggtttttccg gcggcgagaa aaagcgcaac gatattttgc
    13381 aaatggcggt gctggaaccg gagttatgca ttcttgatga gtcggactcc gggctggata
    13441 ttgacgcatt aaaagtggtc gccgatggcg tgaactcgct gcgtgatggc aagcgctcat
    13501 tcatcattgt tacgcactac caacgcattc tcgactacat caagcctgat tacgttcatg
    13561 tgctatatca gggacgaatt gtgaaatccg gcgatttcac gttggtcaaa caactggagg
    13621 agcagggtta tggctggctt accgaacagc agtaatgcgc tgcaacagtg gcatcacttg
    13681 tttgaagctg aagggacaaa acgctcaccg caagcacagc agcatttaca acaattgctg
    13741 cgtaccggac tgccgacacg taaacatgaa aactggaaat atacgccgct ggaagggctg
    13801 accaatagtc agtttgtcag cattgcggga gatatatccc cacagcagcg tgatgcctta
    13861 gcgttaacgt tagacgccgt gcggctggtg tttgtcgatg gacgttacgt gcccgcgctg
    13921 agcgatgcaa ttgaaggcag cggatatgaa gtgagcatta acgacgaccg tcaggggtta
    13981 cccgacgcta ttcaggcgga agtgtttctg catttgacgg aaagcctggc acaaagcgtg
    14041 acgcatatcg ccgtgaagcg cggtcaacgg ccggcaaagc cattgctgtt aatgcatatc
    14101 acccagggcg tggcaggtga agaggtgaac actgcccatt accggcatca tctggatctg
    14161 gcggaaggtg ccgaagcaac ggtgatcgaa cattttgtca gccttaatga tgctcgtcat
    14221 tttaccgggg cacggttcac tatcaatgtc gcagcgaacg cccacttgca gcatatcaag
    14281 ctggcgtttg aaaacccggt cagtcaccac tttgcccata acgatttgtt gctggctgac
    14341 gatgccaccg catttagcca tagtttcctg ctgggtggcg cagtgttacg acacaacacc
    14401 agtacgcaac tcaatggcga aaacagcacg ctgcggatca atagcctggc gatgccggtg
    14461 aaaaacgagg tgtgtgatac ccgtacctgg ctggaacaca ataaaggttt ttgtaacagc
    14521 cgacagttgc acaaaactat cgtcagcgac aaaggccgcg cggtatttaa cggtttgatc
    14581 aacgtcgcgc agcacgccat caaaacggat ggtcagatga ccaacaataa tctgctgatg
    14641 ggcaaactgg cggaagtgga tacaaaaccg cagctggaaa tctatgcaga tgatgtgaaa
    14701 tgcagccacg gcgcgacggt ggggcgtatt gatgatgaac agatgttcta tctgcgctcg
    14761 cgcgggatca atcagcagga tgcccagcag atgatcattt acgcctttgc tgccgaactg
    14821 acggaagcac tgcgtgatga ggggcttaaa cagcaggtgc tggcccgaat cggtcaacgg
    14881 ctgccaggag gtgcaagatg actttttccg tcgacaaagt gcgggccgac tttccggtgc
    14941 tttcgcgtga ggtaaacggt ttgccgctgg cttatctcga cagcgccgcc agtgcacaga
    15001 aaccgagcca ggtgattgac gccgaggccg agttttatcg tcatggctac gcggcggtgc
    15061 atcgcggtat tcatacctta agcgcccagg cgaccgagaa aatggagaac gtgcgcaagc
    15121 gggcatcgct gtttattaat gcccgttcgg cggaagagct ggtgtttgtc cgcggcacga
    15181 cggaagggat caatctggtc gccaatagct ggggcaacag caatgtgcgg gcgggcgata
    15241 acatcatcat cagtcagatg gagcaccacg ctaacattgt cccctggcag atgctttgcg
    15301 cacgcgttgg cgcagagctg cgtgtgatcc ctctcaaccc cgacggtacg ctgcaactgg
    15361 agacgctgcc tacgctgttt gatgagaaaa ctcgcctgct ggcaattact catgtctcca
    15421 acgtgcttgg cacagaaaat ccactggcgg aaatgatcac gcttgcgcac cagcatggcg
    15481 caaaagtgct ggtggatggc gctcaggcgg tgatgcatca tccggtggat gttcaggcgc
    15541 tggattgcga cttttacgtg ttctccgggc ataaactgta tggccccacc ggaattggca
    15601 ttctttatgt gaaagaagcc ttgttgcagg agatgccgcc gtgggaaggg ggcggttcta
    15661 tgatcgccac cgtcagcctg agtgaaggca ctacctggac caaagcacca tggcggtttg
    15721 aagccggtac acccaatacc gggggcatca ttggtcttgg cgcggcgctg gagtatgttt
    15781 cggcgctggg gcttaatagc atagccgagt atgaacagaa tctgatgcat tatgcgctat
    15841 cacagctgga atctgtaccg gatctcactc tctatggcca acaaaacagg cttggcgtta
    15901 ttgcttttaa tctcggtaaa caccacgcct atgatgttgg cagttttctc gataattacg
    15961 gcattgctgt gcgtaccgga catcactgcg ctatgccatt aatggcctat tacaacgtcc
    16021 ctgcgatgtg tcgggcgtcg ctggccatgt ataacaccca tgaagaagtg gagcgtctgg
    16081 tgaccggcct gcaacgtatt caccgtctgc tgggataaca gggaggcact atggctttat
    16141 tgccggataa agaaaaattg ctgcgtaatt ttttacgctg cgccaactgg gaagagaaat
    16201 atctctacat tattgagctg ggccagcgtc tgccagaatt acgcgccgaa gacggaagcc
    16261 cacaaaatag cattcagggc tgccagagtc aggtgtggat tgtcatgcgc cagaatgcgc
    16321 agggaattat tgaattgcag ggcgacagcg atgcggcgat tgtgaaaggg cttattgcgg
    16381 tcgtctttat tctctacgat cagatgacgc cgcaggatat tgtcaatttc gatgtgcgtc
    16441 cgtggtttga aaaaatggcg ctcacccaac atctcacccc atctcgttca caaggtctgg
    16501 aagcgatgat tcgcgcaatt cgcgccaaag ccgctgcact tagctaaact aaagggacag
    16561 ctttcatccg gttggctcac attgccggat ggtcaatgtt tacactgacc ggcacaggag
    16621 tgttttggtt tcaggtgaac ataagcacca tccgacgttt cagcgtgagt ctccggcaaa
    16681 tacaggaggt ttacaatgaa acgcgcgtct ttgcttacac tcacgcttat cggcgctttt
    16741 agcgccatcc aggctgcctg ggcggttgat tatccgctac caccaaccgg aagccgactg
    16801 gttgggcaaa atcaaacgta tacggtgcaa gaaggggata aaaaccttca ggctattgcc
    16861 cgacgttttg atactgcagc aatgttgatc cttgaagcca ataacactat cgccccggtg
    16921 ccaaaacctg gtacgacgat aactattcct tcacaactgt tattacctga tgcaccgcgt
    16981 caggggatta tcgttaacct tgcagagctg cgcctttatt attatccgcc gggagaaaat
    17041 attgtgcagg tttatccaat aggtattgga ttgcaggggc tggaaacgcc ggtgatggaa
    17101 acgcgtgttg ggcagaaaat ccctaaccca acctggacgc ctacggcagg cattcgtcag
    17161 cgttcgctgg agcgtggcat taaattaccg ccagtcgttc ctgccggacc aaataacccg
    17221 ctaggacgtt acgcactgcg cctcgcgcat ggtaatggcg aatacctcat tcatggcacc
    17281 agtgcgccgg acagcgttgg tttgcgcgtc agttcagggt gtattcgcat gaatgcaccg
    17341 gatattaaag ccttgttctc cagcgtgcgg acgggaacgc cggtgaaagt gatcaacgaa
    17401 ccggtgaaat attccgtgga acctaacggg atgcgttatg ttgaagtaca tcgaccacta
    17461 tcagcagaag aacagcaaaa cgttcagaca atgccataca cactgccagc aggctttacg
    17521 caatttaaag acaataaggc tgtagatcag aagttagtcg ataaagcgtt gtatcgtcgg
    17581 gcagggtatc cggtttcggt gagcagtgga gcaactcccg cagccagcaa tgcgccttca
    17641 gtagagtcag cgcagaatgg tgaaccagag caagggaata tgttacgcgc gacgcagtag
    17701 tggtaaatag cagacaaaaa aatggcgcac aatgtgcgcc atttttcact tcacaggtac
    17761 tattacttgc ggtatttagt agccatgttg tccagacgct ggttagcacg agctgcgtca
    17821 tctttagcag cctgaacgtc ggaacgcatt gcgttcacgt cgttgctcag ctggtcaact
    17881 ttagcgttca gagtctgaac gtcagaagac agctgatcga ttttagcgtt gctggagcaa
    17941 cctgccagca gagtagaacc caggattacc gcgcccagta ccagtttagt agctttcatt
    18001 attaataccc tctagattga gttaatctcc atgtagcgtt acaagtatta cacaaagttt
    18061 tttatgttga gaatattttt ttgatgggaa ggcacttatt tttgatcgtt cgctcaaaga
    18121 agcatcgaac gctgggtttt agataaaaaa attgagagaa aacaggatgc ttccatcgga
    18181 ttcatcttaa ataaagtgag attatatagt gaaatccgat ttgatttttt attgcttctg
    18241 gttatcgatt aaataaaaaa agcgcccatc agggcgcttc gatatacaaa ttaattcaca
    18301 aaagcaatat tacaggacgt gaacagatgc ggtgttagta gtgccgctcg gtaccagtgc
    18361 accagaaacc ataactacga cgtcaccttt gtgtgccaga ccgctctgca gagccagttc
    18421 tttacccaga cggtagaaat catcagtaga agtgatctct ttaaccagct gcggcacaac
    18481 gcctttgctc agtaccaact gatgagccgt tttttcgttg gtggtcagtg ccaggatggt
    18541 ggcatccggg aagtatttac gtactgcgcg agcagattta ccgccctggg tagcaaccac
    18601 gatcagcgga gcatccagtt tttcagcagt ttcaacggca ccacggcata ccgcttcggt
    18661 gatgcgcagt ttacggttgt cattgttgaa ctcgagacgg ctgttcatca cgcggtcggt
    18721 acgttcgcag atggtcgcca tgatagaaac cgcttccagc gggtatttac cttttgcgga
    18781 ttcaccagac agcatcactg cgtcagtacc gtcgaggatg gcgtttgcaa cgtcaccggc
    18841 ttctgcgcga gtcgggcgtg ggtttttgat catggaatcc agcatctggg tcgcagtgat
    18901 aacgacttta cgtgcacgga tacatttttc gatcatcatc ttctgggcga agataacttc
    18961 ttctaccggg atttctacac ccaggtcgcc acgcgcaacc atgatgccgt cagaggcttc
    19021 gaggatttcg tcgaagttgt tgaggccttc ctggttttcg attttggaga tgatgtggat
    19081 gttttcgccg ccgtgcgctt tcaggtgctc acggatttcg ataacgtcag aacgcttacg
    19141 aataaaggaa gcagcaacaa agtctacgcc tttttcgcaa ccaaagatca ggtcctgttt
    19201 gtctttttca gccagtgctg gcagagcaat ggaaacgcca ggcaggttca cacctttgtt
    19261 ttcgcccagg tcaccgttgt tcagcacttt acagataact ttgttacctt caatggcggt
    19321 aacttccata ccgatcagac catcgtcaac cagtacggtg ttgccaacag acaggtcagt
    19381 agtgaaacct tcatacgtta ccgcaaccat ttcgctgttg ccgataacag atttatcagt
    19441 ggtgaaagta aaggtctgac cagctttcag agaaacatcg ttaccgcctt ccagtttcat
    19501 ggtgcggatt tccggacctt tggtatcaag caggatagcg gcggttttac cagttttgct
    19561 catcacgttg cgcagattct gaatgcgctg accgtgttct gcatagtcac catgagagaa
    19621 gttcagacgc ataacgttca tgccagcgtc cagcatttta gctaacatct cttcagattc
    19681 ggttttcggt ccgatggtgc aaacaatttt ggtctttttc atgacagtct tagtctttaa
    19741 gttgagaagg atgggagaaa cttgctttct gggcgcactg ccggaaagcg aatccggtat
    19801 tacgaaagtg gtggtgcctc gctctaagga taggtgacat cgaaaaagcg tgcagaggaa
    19861 tgtgtgcttg tgtctcagcc caacgtgagt gtctgttttt gagtcgttga gttctacagt
    19921 ccaaagtgct gaaaccattc aagagtcaat tggcgcgcat tatacgc
//
LOCUS       DAJTYM010000076        19920 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTYM010000076 DAJTYM010000000
VERSION     DAJTYM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..199
                     /gene="raiA"
                     /locus_tag="OO575_002759"
     CDS             <1..199
                     /gene="raiA"
                     /locus_tag="OO575_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6665662.1"
                     /translation="VADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKG
                     EARRAATSVKDANFVEEVEEE"
     misc_feature    302..427
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            303..350
                     /gene="pheL"
                     /locus_tag="OO575_002760"
     CDS             303..350
                     /gene="pheL"
                     /locus_tag="OO575_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6665663.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            449..1609
                     /gene="pheA"
                     /locus_tag="OO575_002761"
     CDS             449..1609
                     /gene="pheA"
                     /locus_tag="OO575_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6665664.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1652..2773)
                     /gene="tyrA"
                     /locus_tag="OO575_002762"
     CDS             complement(1652..2773)
                     /gene="tyrA"
                     /locus_tag="OO575_002762"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6665665.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2784..3854)
                     /gene="aroF"
                     /locus_tag="OO575_002763"
     CDS             complement(2784..3854)
                     /gene="aroF"
                     /locus_tag="OO575_002763"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6665666.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4064..4429
                     /locus_tag="OO575_002764"
     CDS             4064..4429
                     /locus_tag="OO575_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6665667.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4579..5097
                     /locus_tag="OO575_002765"
     CDS             4579..5097
                     /locus_tag="OO575_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6665668.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5087..6312
                     /gene="dgcN"
                     /locus_tag="OO575_002766"
                     /pseudo
     CDS             5087..6312
                     /gene="dgcN"
                     /locus_tag="OO575_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6328..6810
                     /locus_tag="OO575_002767"
     CDS             6328..6810
                     /locus_tag="OO575_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6665669.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(6887..7234)
                     /gene="rplS"
                     /locus_tag="OO575_002768"
     CDS             complement(6887..7234)
                     /gene="rplS"
                     /locus_tag="OO575_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6665670.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7276..8043)
                     /gene="trmD"
                     /locus_tag="OO575_002769"
     CDS             complement(7276..8043)
                     /gene="trmD"
                     /locus_tag="OO575_002769"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6665671.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8074..8622)
                     /gene="rimM"
                     /locus_tag="OO575_002770"
     CDS             complement(8074..8622)
                     /gene="rimM"
                     /locus_tag="OO575_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6665672.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8641..8889)
                     /gene="rpsP"
                     /locus_tag="OO575_002771"
     CDS             complement(8641..8889)
                     /gene="rpsP"
                     /locus_tag="OO575_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6665673.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9026..10387)
                     /gene="ffh"
                     /locus_tag="OO575_002772"
     CDS             complement(9026..10387)
                     /gene="ffh"
                     /locus_tag="OO575_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6665674.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10554..11345
                     /locus_tag="OO575_002773"
     CDS             10554..11345
                     /locus_tag="OO575_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6665675.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11366..12652
                     /locus_tag="OO575_002774"
     CDS             11366..12652
                     /locus_tag="OO575_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6665676.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12707..13300)
                     /gene="grpE"
                     /locus_tag="OO575_002775"
     CDS             complement(12707..13300)
                     /gene="grpE"
                     /locus_tag="OO575_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6665677.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13423..14301
                     /gene="nadK"
                     /locus_tag="OO575_002776"
     CDS             13423..14301
                     /gene="nadK"
                     /locus_tag="OO575_002776"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6665678.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14387..16048
                     /gene="recN"
                     /locus_tag="OO575_002777"
     CDS             14387..16048
                     /gene="recN"
                     /locus_tag="OO575_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6665679.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16197..16538
                     /gene="bamE"
                     /locus_tag="OO575_002778"
     CDS             16197..16538
                     /gene="bamE"
                     /locus_tag="OO575_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6665680.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16600..16890)
                     /locus_tag="OO575_002779"
     CDS             complement(16600..16890)
                     /locus_tag="OO575_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6665681.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(16880..17317)
                     /gene="ratA"
                     /locus_tag="OO575_002780"
     CDS             complement(16880..17317)
                     /gene="ratA"
                     /locus_tag="OO575_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6665682.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17488..17970
                     /gene="smpB"
                     /locus_tag="OO575_002781"
     CDS             17488..17970
                     /gene="smpB"
                     /locus_tag="OO575_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6665683.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18185..18547
                     /gene="ssrA"
                     /locus_tag="OO575_002782"
     tmRNA           18185..18547
                     /gene="ssrA"
                     /locus_tag="OO575_002782"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            18752..>19920
                     /locus_tag="OO575_002783"
     CDS             18752..>19920
                     /locus_tag="OO575_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6665684.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWA"
BASE COUNT         5110 a         5234 c         4684 g         4892 t
ORIGIN      
        1 tgttgctgac gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga
       61 agatatgtac accgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact
      121 gcagcacaaa ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga
      181 agaagttgaa gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg
      241 ttttttgttg acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa
      301 acatgaaaca cataccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg
      361 tttcgtcgtg tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct
      421 tttttattga taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg
      481 agagaaaatc agcgcgctgg atgaaaaatt attagcgtta ctggcagaac ggcgcgaact
      541 ggccgtcgag gtgggaaaag ccaaactgct ctcgcatcgc ccggtacgtg atattgatcg
      601 tgaacgcgat ttgctggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca
      661 ttacattact cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt
      721 gctccaacaa catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc
      781 caaaggttct tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt
      841 cattgaaagt ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc
      901 cgactatgcc gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga
      961 tctgctgcaa cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg
     1021 tttgttggtc tccggcacta ctgatttatc caccatcaat acggtctaca gccatccgca
     1081 gccattccag caatgcagca aattccttaa tcgttatccg cactggaaga ttgaatatac
     1141 cgaaagtacg tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc
     1201 gttgggaagc gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc
     1261 aaatcagcga caaaacttca cccgatttgt ggtgttggcg cgtaaagcca ttaacgtgtc
     1321 tgatcaggtt ccggcgaaaa ccacgttgtt aatggcgacc gggcaacaag ccggtgcgtt
     1381 ggttgaagcg ttgctggtac tgcgcaacca caatctgatt atgacccgtc tggaatcacg
     1441 cccgattcac ggtaatccat gggaagagat gttctatctg gatattcagg ctaatcttga
     1501 atcagcggaa atgcaaaaag cattgaaaga gttaggggaa atcacccgtt caatgaaggt
     1561 attgggctgt tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc
     1621 cggatgatgt gaatcatccg gcactggatt attactggcg attgtcattc gcctgacgca
     1681 ataacacgcg gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct
     1741 tgcggaaact gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc
     1801 cgaaacgctt atagtaacgt ttgattaacg ccagattacg ctctgacgac ataatgatgt
     1861 cggcataaag ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt
     1921 aaatcggcga agagagcgcc agaagttgct caagctgaac attttcttct gccaggtgca
     1981 gcccgtaagc aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt
     2041 gctcgacagc gctaatacga tgcaaccgag cgccccagac ctgaatttgc tccagaaacc
     2101 attggtatgc ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac
     2161 cgctgtccgg gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca
     2221 tggcctgtaa tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta
     2281 aaggcggtaa tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca
     2341 ttccggcatc ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca
     2401 cctgataacc cgatagtgtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc
     2461 cgacgataac caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac
     2521 tggagtaaga ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac
     2581 ccagagcttc cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat
     2641 aaataggcag tccaaagcgg cttttcacct tgcccacttc agcaaccagt tccagacgct
     2701 tcgctaataa attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca
     2761 attcagcaac cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc
     2821 tgatgaattt cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg
     2881 gatacaccgt atttcatttc actgcgcggt tgctcagaag actgattgcc ctcgtggata
     2941 ttactttcga tcatcagacc aataattgag cgattaccat ctttgatttg agcaaccacg
     3001 gattctgcca ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct
     3061 accatcagag acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc
     3121 gcagggctat agttcggcgc tttaccaccg cgcaggatca catgcccgtc cggattcccc
     3181 tgagtttgta gcaacgcaac ctgccctgcc tggttaatgc caacaaaaca gtgcggctgg
     3241 gcggcggcgc gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa
     3301 ccaaccggca tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga
     3361 gcaccaattg ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac
     3421 gcttccgtcg ccagtggcag tcccatattc accagctcaa gcaacaattt acgcgcgatt
     3481 tgcagcccgg cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc
     3541 cagccgacag tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg
     3601 ctgacctctg cggcaagagc tttaaatcga cgagcatatt ccagagcagt ttccggatca
     3661 tgaatggaac aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct
     3721 gaaatggttt tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg
     3781 gccttcagtt gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc
     3841 gcgtctttat gcatgatggc gatcctgttt atgctcgttt gcgatagttg ctcctcagcg
     3901 aggatgacgt aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta
     3961 tttacacagg caatttagtc gcgctttcaa cccttgcttc tgtatagata aatttacatc
     4021 ccctttaaaa acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat
     4081 cgcccccttg ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc
     4141 aaccttgacc agcaccgact ggtatgatgt cggtatggaa gatgcgatat cgggcagcgc
     4201 cataaaagat gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg
     4261 atatgccgaa gggcaaaaga aaacttgcca gaccgatttt acttatgccc ggggactttc
     4321 cggtaaaagc tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt
     4381 atggcaaaaa agggctgatg aaaatgccag cacaatacga ttaaattaat taaaacccca
     4441 caaataaatt aaacataaga ttttactcat tgtgttttat ttctcacatt gatgacggtc
     4501 gcatgaaaaa atgatatttt aatgagcatc ctgaaaaaat atcgatgcat ttcgagcgaa
     4561 gatggtgggg atccctgaat gcgtttttct caccgactgt tcttacttct gattctcctg
     4621 ctgacgggag caccaatact cgctcaagag ccttctgacg ttgcgaaaaa cgtgcgtatg
     4681 atggtttccg gtattgtcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta
     4741 tgtatctttt catcttcacg ttttagtacc gccctacaag agaatgccgc gacatctttg
     4801 ccttatctgc ctgtcatcat tcacactcaa caagaggcga tgatttcagg ctgcaatggt
     4861 ttttattttg gcaacgagtc accgacattc cagatggaat taaccgaaca atatccgtca
     4921 aaggcgttat tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt
     4981 ctgatcatcc acaataatga cgtcagattt gccgtaaacc tggatgcctc atcgcgtagc
     5041 ggtgtaaagg tcaacccgga tgtcttaatg ctcgcacgga agaaaaatga tggataacga
     5101 taattctctt aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag
     5161 catatttatc actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct
     5221 gaaacagtac gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga
     5281 agcagctgtc gtttttgccg atggccctgc agcaactgaa acactggcag cgctgggcca
     5341 gcaagggcat tttcaactgc agaagtacgt gataagcagc aaaatattct ggcatcctgg
     5401 cattaccccc gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc
     5461 cctgccccca tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta
     5521 accgctcgcg acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt
     5581 tgtattctgc tggcatcagg catcgcaatt accctcaccc gccatttgca caatggcctg
     5641 gtggaagcac tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt
     5701 tcccgacgag tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac
     5761 agtctgctgg atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt
     5821 ctgcgtaccg cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc
     5881 ggcatcaata cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt
     5941 gatggcgata atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagggtc
     6001 ttgattgaaa tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc
     6061 ctgggcggcg atgaattcgc tatggtgctc tatgatgtac agtcggaatc tgaagtgcag
     6121 cagatatgct cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggccat
     6181 cagaccacca tgacattaag cattggttac gcgatgacca ttgagcacgc ctctgcggaa
     6241 aaattacaag agcttgccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag
     6301 ctggtgagat aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc
     6361 acctcactaa tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc
     6421 gccgctatgc aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca
     6481 gatgccattc tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc
     6541 caaaccatgg cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac
     6601 accgataact atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc
     6661 gtggccgatg catgggctat tggtggacaa attccacgca gcaatctcac cacacagggt
     6721 ttaggaaaaa aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc
     6781 cgcgtcgcag tggtgattgc aaccccttaa aacacgagca aacagacaaa aaaagggcca
     6841 gccaattggc cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct
     6901 ctttgatacg agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa
     6961 cagcaccacg acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac
     7021 gctcaacgcc ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt
     7081 tacgaatagc gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa
     7141 cccatacttt cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct
     7201 gctcttgttc aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac
     7261 tgatctctcg ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt
     7321 tttgaactcc gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc
     7381 aggtcttcta agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc
     7441 atggttgccc gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt
     7501 atagtgcggg caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg
     7561 tcccagtact cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc
     7621 accaccactg agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac
     7681 gcgctcatct ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag
     7741 ttcgctgacg cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc
     7801 gccttcaccc gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat
     7861 taacatcccc ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt
     7921 gaagtcgcga ggactccaac tctggatgct cagcaggcca tttttaactg cccggccagt
     7981 taccccgtaa tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca
     8041 catagcgccg ccttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc
     8101 gattgaacga gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag
     8161 acgttccttg ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattaga
     8221 tccggtttcc atcatatcaa cgactttacc gagatcgtag ccttcagtgg ttactacctg
     8281 gcagcccatc aggtctttcc agtagtagtc cccctcttca agctgaggca gctgcgatga
     8341 atccacgaca atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt
     8401 cagcttgatg atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca
     8461 ctgacccgcc ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt
     8521 ggaagaaaac actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac
     8581 gatgggatca acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga
     8641 ttaagctgct ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc
     8701 ctggccaacc cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct
     8761 agcgattggg ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct
     8821 gtcagcgaca acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat
     8881 agttaccata acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc
     8941 cggtgtcata ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt
     9001 taataactct gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcattccct
     9061 tcatgcttct catcatcttc gccattccgc ctttcttcat tttcttcatc atgcgctgca
     9121 tgtcgtcgaa ctgcttcaga aggcggttaa cgtcctgtac ctgcatcccg caaccggcgg
     9181 cgatacgacg cttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca
     9241 ttgagttgat gatggcttcc atacgcacca gcactttatc gtccatctgc gacttgacgt
     9301 tatccgggat ctgccccatg cccggcagtt tgcccatcaa gctggccatg ccgcccatat
     9361 ttttcatctg gcgcagttgc tcaagaaagt cgttgagatc gaagccgtcg ccttttttta
     9421 gcttgctggc taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca
     9481 gcgacagtac gtcgcccatg ccgagaatac gcgacgcgat gcggtccgga tggaacggct
     9541 ccagcgcctc agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac
     9601 gaatagagag cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg
     9661 taagcggtaa cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg
     9721 cgtcaaccac aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt
     9781 ccatcatcgc ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga
     9841 atttcagttt ggcttctttc agcgccgcgt taacaatatc aaccggcttc tgaccaacat
     9901 cagaagggaa gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg
     9961 ccgggcgata gacgtcagca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga
    10021 aattaccgag tttaccaacg ctggttgttt taccggcacc ttgcaggcct gccatcagca
    10081 cgaccgcagg cggttgcgca gccagattca gggtctggtt ctcttcgccc atcgccgcaa
    10141 ccagttcgtt acggacgatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt
    10201 catgaccaac cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga
    10261 cgtccgcctc cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag
    10321 tgaggcgtcc acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat
    10381 caaacattgt ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt
    10441 attttgccgc agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt
    10501 cacctgacgc tatactgctt ctctttctta ttgctcaaac tgtcgacatt actatgcccg
    10561 tttttgctct gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc
    10621 tgctgcaaaa aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg
    10681 tctgccacgc aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc
    10741 tcagcctgct gaacgttggt tcattggtca gtttggtgat ctgtacggta atgaccattg
    10801 tggcttctcg caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca
    10861 acctggcgct ggcgaccttc atgcccaatg aatacatcac ccatctggaa gcaacgcctg
    10921 ggatgctgat gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc
    10981 tgtacgcgct gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcattta
    11041 accaagaaat gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg
    11101 gcgtggtgct gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacctattta
    11161 gcatggaaaa tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc
    11221 tgctgtgggg acattaccat gaaggatggc gtggacgccg cgtcgtctgg tttaacgttg
    11281 cgggcgcggt aattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca
    11341 gctaaaccca gaaaaggagt ttcccctgga acacatttct actactacgt tgatcattat
    11401 tctgatcatc atggtggtca tttcagccta tttttccggg tccgaaaccg gaatgatgac
    11461 cctcaaccgc tatcgtcttc gacatatggc gaaacagggt aatcgctcgg ccaaacgcgt
    11521 cgaaaaattg ctgcgtaagc cagaccgcct gataagcctg gtgttaatcg gcaataacct
    11581 ggtcaatatt cttgcctccg cgctcggcac tattgtcggg atgcgtttgt acggcgatgc
    11641 gggcgtggca attgcgactg gtgtgctgac ttttgtcgta ctggtttttg ctgaggtatt
    11701 gccgaaaacc attgccgcgc tgtacccgga aaaagtcgct tatccgagta gttttctgct
    11761 ggctccgctg caaattttga tgatgccgct ggtctggttg ctgaatgcta tcacccgtat
    11821 gctgatgcgc atgatgggta tcaaaaccga tatcgtggtt agcggctctt tgagcaaaga
    11881 agagttgcgc actatcgtgc atgaatcgcg ctcacaaatt tcccgtcgca atcaggatat
    11941 gctgctgtcg gtgctcgatc tggaaaaaat gaccgttgat gacatcatgg taccgcgcag
    12001 tgaaattatc ggtattgata tcaacgatga ctggaaatct attctgcgcc aactctccca
    12061 ctcacctcac gggcgcatcg tgctttaccg tgattcgctg gacgacgcca tcagtatgct
    12121 gcgagtacgt gaagcctggc ggttgatgtc ggagaaaaaa gagttcacca aagaaaccat
    12181 gctgcgcgcc gcggacgaga tctattttgt gcctgaaggt acgccgctca gcacgcagtt
    12241 ggtaaagttt cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat
    12301 tcaggggctg gtgacggttg aagatattct ggaagagatt gttggcgatt tcactacgtc
    12361 gatgtcgcca acacttgccg aagaggtcac gccgcaaaac gacggttcgg tgattatcga
    12421 tggcaccgcc aacgtgcggg aaatcaacaa agcctttaac tggcatctac cggaagatga
    12481 tgcccgcacg gttaatggcg tcattcttga ggcactggaa gagatccctg tcgcaggcac
    12541 ccgcgtgcgt attggcaagt acgatatcga tattctcgac gtacaggaca atatgattaa
    12601 gcaggtaaaa gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt aacgaaaacg
    12661 gccctgcatt cgcatgcagg gccgtgaatt attacgaaaa cagaaattac gcttttgctt
    12721 tcgctacagt taccatcgcc gcacgaatcg tacgaccatt cagcgtataa cccttctgca
    12781 taatgcccag tacgttacct ggcgcaacgt catcagattc caccattgcg atagcctgat
    12841 gcacattcgg gtccagcggc acgttagttt cggcgatcac ttcaacacca aacttacgca
    12901 caacatccag catcgacttc agcgtcagct caatgccttc aaccatcgca gacatatccg
    12961 ggttagcttt atcagccact tccagcgcac gatccaggct atcaatcacc ggcagcaatt
    13021 cgttgatgaa tttctccagc gcgaatttgt gggctttttc aatatccagt tcagtacgac
    13081 gacgcaggtt ttccatttcg gcttttacgc gcaaaatacc gtcacgttca cgggtctggg
    13141 cttcagccag ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc acctgctcag
    13201 cagaagcttc tggctcaact gcctcaatct cttcgtgctg atccatgata atttcttccg
    13261 gggcttgccc ctcaggcgtt ttctgttctt tactactcat gaatttctcc gcgttttttt
    13321 cgcattcatc tcgctaactt cgcttattat ggggatcagt ttcagggttt caagggaagc
    13381 actcacattg tcatcaatct tcgcaacaag gacctcggaa aaatgaataa tcatttcaag
    13441 tgtattggca ttgtgggaca cccacggcac cctactgcac tgacaacaca tgaaatgctc
    13501 taccgctggc tgtgcacaaa aggttacgag gtcatcgttg agcaacaaat cgctcacgaa
    13561 ctgcaactga agaatgtgaa aactggcacg ctcgcggaga ttgggcaact ggctgatctc
    13621 gcggtagtcg ttggtggcga cggtaatatg ctgggcgcgg cgcgcacgct cgcccgttac
    13681 gatattaaag ttattggaat caaccgtggc aacctgggtt tcctgactga ccttgacccc
    13741 gataacgccc agcaacaatt agccgatgtg ctggaaggtc actacatcag cgagaaacgt
    13801 tttttgctgg aagcgcaagt ctgtcagcaa gattgccaaa aacgcatcag caccgcgatt
    13861 aacgaagtgg tacttcatcc tggcaaagtg gcgcatatga tagagttcga agtgtatatc
    13921 gacgagatct ttgcgttttc tcagcgatct gatgggctaa ttatttcgac gccaacaggc
    13981 tccaccgcct attccctctc tgcgggcggt cctattctga ccccctctct ggatgcgatt
    14041 accctggtgc ccatgttccc gcatacgttg tcagcacgac cactggtcat aaacagcagc
    14101 agcacgatcc gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc
    14161 cagatagcac tgccgattca ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat
    14221 ctgaatctga ttcatccgaa agattacagt tatttcaata cattaagcac caagctcggc
    14281 tggtcaaaaa aattattcta attttacgcc agcctcttta ctgtatataa aaccagttta
    14341 tactgtacac aataacagta atggtttttc atacaggaaa acgactatgt tggcacaact
    14401 gaccatcagc aactttgcta tcgttcgtga gcttgagatt gattttcata gcggcatgac
    14461 cgtaataact ggcgagaccg gtgcgggtaa atctattgca atagatgccc tcggtctttg
    14521 tctcggtggt cgcgctgaag ccgacatggt gcgtaccggc gctgctcgcg ctgacctgtg
    14581 cgcccgtttt tctctgaaag atacgccagc ggccctgcgc tggctggaag aaaaccagct
    14641 tgaagacggg catgaatgtt tgcttcgtcg cgtgatcagc agtgatggtc gctcccgtgg
    14701 tttcatcaac ggtacagctg ttcctctgtc acaactgcgc gaactgggtc agttgctgat
    14761 tcagatccat ggtcagcacg ctcatcaatt actcaccaaa cctgagcacc aaaaattcct
    14821 gcttgatggc tatgccaatg aaacctctct acttcaggaa atgaccgcac gttatcagtt
    14881 gtggcatcaa agctgccgtg acctcgcgca tcatcaacag ttaagtcagg aacgcgccgc
    14941 ccgtgcggaa ctgctgcaat accaattaaa agaacttaac gaatttaatc cgcagcccgg
    15001 agagtttgag caaatcgacg aagagtacaa acgtctggcg aacagcggtc aattgctgac
    15061 caccagccag aatgcattgg cattaatggc cgacggtgaa gacgcaaacc tgcaaagtca
    15121 gctttacacg gctaaacaac tggtgagcga attgattggc atggacagca aactgtccgg
    15181 cgtacttgat atgctggaag aagctaccat ccagattgct gaagccagcg acgaactgcg
    15241 ccactactgc gatcgtctgg atctcgatcc caaccgactg tttgaacttg aacagcgcat
    15301 ctcaaaacag atttcgctgg cacgtaaaca tcacgtcagc cctgaggcat taccacagta
    15361 ttaccagtcg ctactggaag aacagcagca actggacgat caggccgact cacaagaaac
    15421 gctcgcgctg gcggtaacga aacatcatca gcaggcactg gaaaccgcgc gcgcattaca
    15481 ccaacaacgc cagcaatatg cagaagaact tgcacagctg atcaccgaca gtatgcatgc
    15541 gctctcaatg ccgcatgggc agtttacgat tgatgtcaaa tatgacgagc atcacctggg
    15601 cgctgacggt gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc agccaatgca
    15661 gcctattgcc aaagtcgcat ccggtggtga attgtcccgc atcgcactgg caatccaggt
    15721 catcacggcg cgtaaaatgg aaaccccggc actgattttt gatgaagtgg atgtagggat
    15781 tagcggtcca acagcggcag ttgtcggcaa actgctgcgt caactcggcg aatcaactca
    15841 ggtgatgtgt gttacccacc tgccacaagt cgcgggatgt ggtcatcaac actattttgt
    15901 cagcaaagaa accgatggtg cgatgacaga aacacatatg caatctctgg ataaaaaagc
    15961 gcggttacaa gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta acacactggc
    16021 gaatgcgaaa gaactgcttg cagcgtaaac ttttttcctg cttcacggtc agagtaaaca
    16081 gcaaaacgcc gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt ctattatcat
    16141 cggcatatta cagatgagcc acgtactgct cgggcccgaa aaggaatcaa atcactatgc
    16201 gctgtaaaac gctgactgct gcagcagcag tactattgat gttaaccgca ggctgttcca
    16261 ctctggagcg agtggtttac cgtcctgaca tcaaccaggg gaactatctg accgctaacg
    16321 acgtatccaa aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac
    16381 cgctgatgtc cgatccattt ggtacgaata cctggttcta tgtcttccgc cagcaaccag
    16441 gtcatgaagg tgtaacgcag caaacgctga cgctgacctt taacagtagc ggtgtgttga
    16501 ccaatattga taacaaacct gcgctgagtg gtaactaata ataaagttgc tctcaaagac
    16561 gttaaaaaag gtgctcaatg agcacctttt ttctgtctgt tatttattcg ctgatttttc
    16621 tgctcgttgc ctgcgaagct ctttcggatc ggcaatgaga gggcgataaa tctccacccg
    16681 atcgccatca tgcacactat cgcttagttt tgccggacgg ctgtaaatgc cgactttgtt
    16741 tttagttaaa tcgatatcgg tacgcaattc cagcaagcca ctggcgcgaa tagcttcttc
    16801 aaccgtcgcg ccctcctgca gcgtcactcg ctgcaggtat tgcttctcag gtagcgcata
    16861 agccacctca acggcaattt tacctagcac tgtaaacctc tttcgcacga accgtaaaag
    16921 cctggaccat attagccgcc agctctttaa acacgcgacc aaaggcgagt tcaatcaact
    16981 tattggtaaa ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg ctcagcggcg
    17041 taaacttcca tccaccaatc aatttcttga acggcccatc caccagattc ataagaatac
    17101 tttggttact ggtcaactgg ttgcgggtag taaacgtttt gctgatccca gccttagaga
    17161 catctaccgc cgcagtcatc tgcccaggag tggactccag aatccgactt ccggtacaac
    17221 ctggcaaaaa ctgaggataa gactgaacgt cattcactaa ctgatacatt tgctccgcgc
    17281 tgtagggtac cagtgcggtc cggctaatct gaggcataac aatttccatc aacaaaaatc
    17341 caacaaataa tatcatttat cctgctgtaa aaaaaacgct atcccggcgc tgggtaacat
    17401 cgggttcatg ctaagataga gccttgtccc ccgcaggatt gatatggggt attttcgatt
    17461 tcagattacc gatgattcac gacgcttatg acgaagaaaa aagcacataa acctggttca
    17521 gcgaccatcg cgcttaacaa gcgcgcccgt cacgaatact ttatcgaaga agagttcgaa
    17581 gcgggacttg ccctgcaagg ctgggaagtt aaatccctgc gcgcaggaaa agccaatatc
    17641 agcgacagct acgtccttct gcgtgacgga gaggcatttc tgtttggcgc taacatcacg
    17701 ccaatggccg tggcctccac gcatgtggtg tgcgatccta cccgtacccg caagttactt
    17761 ctcaaccagc gcgaactgga ctcattgtac ggtcgcgtca atcgagaagg ctataccgta
    17821 gtggcgctct ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa
    17881 ggtaaaaaac agcacgataa acgttcagat atcaaagaac gcgaatggca ggtggataaa
    17941 gcacgcatca tgaaaaacgc ccaccgttaa acctgcactc caattattga ccagttcctc
    18001 accgcgcctc cctctccggc ggcgcgaatg aacatcttat tggctatcac atccgacaca
    18061 aatgttgcca tcccattgct taatcgaata aaaatcaggc tacatgggtg ctaaatcttt
    18121 aacgataacg ccattgaggc tggtcatggc gctcataaat ctggtatact tacctttaca
    18181 cattggggct gattctggat tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc
    18241 ggttggcctc gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta cgctttagca
    18301 gcttaataac ctgcttagag ccctctctcc ctagcctccg ctcttaggac ggggatcaag
    18361 agaggtcaaa cccaaaagag atcgcgtgga agccctgcct ggggttgaag cgttaaaact
    18421 taatcaggct agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa tgtaaagact
    18481 gactaagcat gtagtaccga ggatgtagga atttcggacg cgggttcaac tcccgccagc
    18541 tccaccaaaa ttctccatcg gtgattacca gagtcatccg atgaagtcct aagagcccgc
    18601 acggcgcaag ccctgcgggc ttttttgtgc tccgaatttg tcccgcgaag tctgatgcca
    18661 actaattaaa tccgatcctt ttaggcacct tgttaggcac ttcataaagc tatattgtct
    18721 ttgaggtgcc taaaactatg gaaacccggc aatggcaaga caaaccaaac ctctatccgt
    18781 taaggaaatc gaatctgcta aacccaagga agcggactac gttctctatg atggcgatgg
    18841 ccttgagcta ctcattaaat ccagcgggag taaaatctgg cagtttcgct acattcgccc
    18901 tgtcaccaag acacgagcaa agaaaagcat aggcccttac ccgtcagtta cccttgccga
    18961 tgccagaaac taccgtgcag agtcccgctc actcctagct aaacagattg atccgcagga
    19021 acatcagcaa gaacaattgc gtagttcgct ggaagccaaa accaatactt tccagctcgt
    19081 agctgaacgt tggtggaatg tgaagaaagc cagtgtgaca gaggactatg cggaagatat
    19141 ctggcgctct cttgaaagag atgtctttcc tgcgattggc gacgttagcg ttacagatat
    19201 taaagctcat acactggttc aggccgtaca accggttcag gccagaggag cactggaaac
    19261 ggttcgtcgc ctgtgtcaac gtattaatga ggtcatgatc tatgcccaga acacagggct
    19321 gattgatgct gttcccagcg ttaatatcgg taaagccttc gaaaagcctc agaaaaagaa
    19381 catgcccagc attcgaccgg atcagctatc tcaactgatg cagacaatgc gaacagccag
    19441 cattagcctt tccacacgct gcctgttcat gtggcaactt cttactatta cccgccctgc
    19501 cgaagcggct gaagctcgct gggaagaagt agacatagaa gcgcaagagt ggaagattcc
    19561 cgcagcacgc atgaaaatga accgcgacca tactgttcca ttgtcagatg aagcaatagc
    19621 tgtgctggag atgatgaaac cgctaagcgg caaccgagaa tttatctttc ctagccgtat
    19681 caagcccaac cagcctatga acagtcagac tgttaacgcc tcactaaaac gcgcaggttt
    19741 tggtggtgtg cttgtttcgc atggtctgag atcaattgcc agtacggctc ttaatgaaca
    19801 aggctttccg cctgatgcca ttgaggcagc attggcccat gtggataaga atgaggttcg
    19861 tcgtgcttat aaccgcagcg attacctgga acagcgtcgc ccgatgatgc aatggtgggc
//
LOCUS       DAJTYM010000077        19578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTYM010000077 DAJTYM010000000
VERSION     DAJTYM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            12..227
                     /locus_tag="OO575_002784"
     CDS             12..227
                     /locus_tag="OO575_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth inhibitor PemK"
                     /protein_id="HCR6665685.1"
                     /translation="MSTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIAR
                     GAKFHTVADEKLISEVISKLVNLIDPQ"
     gene            complement(228..1016)
                     /gene="bioH"
                     /locus_tag="OO575_002785"
     CDS             complement(228..1016)
                     /gene="bioH"
                     /locus_tag="OO575_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6665686.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1054..1737
                     /gene="gntX"
                     /locus_tag="OO575_002786"
     CDS             1054..1737
                     /gene="gntX"
                     /locus_tag="OO575_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6665687.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            1796..2371
                     /gene="nfuA"
                     /locus_tag="OO575_002787"
     CDS             1796..2371
                     /gene="nfuA"
                     /locus_tag="OO575_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6665688.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            2732..4048
                     /gene="gntT"
                     /locus_tag="OO575_002788"
     CDS             2732..4048
                     /gene="gntT"
                     /locus_tag="OO575_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6665689.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4093..6177)
                     /gene="malQ"
                     /locus_tag="OO575_002789"
     CDS             complement(4093..6177)
                     /gene="malQ"
                     /locus_tag="OO575_002789"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6665690.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6187..8580)
                     /gene="malP"
                     /locus_tag="OO575_002790"
     CDS             complement(6187..8580)
                     /gene="malP"
                     /locus_tag="OO575_002790"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6665691.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9192..11897
                     /gene="malT"
                     /locus_tag="OO575_002791"
                     /pseudo
     CDS             9192..11897
                     /gene="malT"
                     /locus_tag="OO575_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(11940..12956)
                     /gene="rtcA"
                     /locus_tag="OO575_002792"
     CDS             complement(11940..12956)
                     /gene="rtcA"
                     /locus_tag="OO575_002792"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6665692.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(12960..14186)
                     /gene="rtcB"
                     /locus_tag="OO575_002793"
     CDS             complement(12960..14186)
                     /gene="rtcB"
                     /locus_tag="OO575_002793"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6665693.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14367..15965
                     /gene="rtcR"
                     /locus_tag="OO575_002794"
     CDS             14367..15965
                     /gene="rtcR"
                     /locus_tag="OO575_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6665694.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(15947..16705)
                     /locus_tag="OO575_002795"
     CDS             complement(15947..16705)
                     /locus_tag="OO575_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6665695.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(16722..17552)
                     /gene="glpG"
                     /locus_tag="OO575_002796"
     CDS             complement(16722..17552)
                     /gene="glpG"
                     /locus_tag="OO575_002796"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6665696.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(17597..17923)
                     /gene="glpE"
                     /locus_tag="OO575_002797"
     CDS             complement(17597..17923)
                     /gene="glpE"
                     /locus_tag="OO575_002797"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6665697.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18113..>19578
                     /gene="glpD"
                     /locus_tag="OO575_002798"
     CDS             18113..>19578
                     /gene="glpD"
                     /locus_tag="OO575_002798"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6665698.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWL"
BASE COUNT         4552 a         5437 c         4960 g         4629 t
ORIGIN      
        1 tgtgctgccc gatttcaaca ggggcaaatg cagcacgttc cacaggggtg acggtgaacg
       61 tcctcccccg tgatacgcaa accggtaacc tgcatggcgt tgtactttgt caccagctaa
      121 aagccgtcga tcttattgcc cgtggcgcta aatttcatac cgttgccgat gaaaaattga
      181 ttagtgaagt tatcagtaaa ctggtgaatt taatcgaccc acaataatta gctctcactt
      241 accaccaaca ccctctgctt caacgccacc agcaggtgac aaaactcgac cggatgcgaa
      301 ataaacggcg catgggccgc tttggcgaag atatatgatt cgctgtgagg ccaaagttta
      361 tccagcatcg gcaccacttt gcgcggcacc agaccgtcga gatagccata caatcgcaaa
      421 aacggcatgg acacgttttg cagcggctga cggagatcga ccgttttcag gatttccagc
      481 ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt cttcaacgcc
      541 cgcgcatcct ggcgcgccgt ttcagtcccc atggtttgta acgccaggaa ccgctccact
      601 gtacgctgaa aatcatcact gagttgctgc tgaaatcccg ccagcacgtc cggttttatc
      661 cccggccact cgtcacgagc actaaaacaa ggtgacgacg ccacggtgac cagcgcctga
      721 acacgctcgg gatgggttaa cgcaatctgg cttgccacca gcccgcccag actccagcct
      781 aaccaaatgg ctttatcagg tgcctgttgc agcacggctt cggccatatc agcaagtgac
      841 agcgcaccaa atccccggct acgcccgaag ccgggcaggt caacaaggtg cagcgtaaaa
      901 tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc
      961 agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt attcatccgc
     1021 tattgttctc ttttgactta caaggatgaa catatgctaa cagtaccggg attatgctgg
     1081 ctatgccgaa tgccactggc gttaggtcat tgggggattt gttcggtctg ttcacgcgcc
     1141 acccgcacag ataaaacgtt atgcccacaa tgtggattac ccgccacaca ctcccatctt
     1201 ccctgcggtc gctgcctgca aaaaccgccg ccctggcaaa gactggtcac ggttgccgac
     1261 tatgcgccgc cgttaagtcc gcttatccac cagcttaaat tttcccggcg cagtgaaatc
     1321 gccagcgccc tgtcacgtct gttactattg gaagtcttac acgctcgtcg caccaccggg
     1381 ttgcaattgc cggatcgcat catcagcgtt ccgttatggc agcggcgtca ctggcgtcgg
     1441 ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggttgca ctgccaatgg
     1501 gatagcgaag ccgtcacacg tacacgggcc actgcgaccc agcattttct cagtgcccgg
     1561 ctgcgcaagc gcaacctgaa aaatgccttt cgtcttgaat tgcccgtgca aggtcgccat
     1621 atggtgattg tggatgatgt cgttaccacc ggaagtaccg tcgcagagat tgcgcagttg
     1681 cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac cttgtagagc
     1741 ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca ttactatgat
     1801 ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaatc aggaagaagg
     1861 gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat gtggtgtttc
     1921 ttattgtccg ccggatgccg tggaagccac cgacactgcc ctgaaatttg acctgctgac
     1981 cgcgtatgtt gatgagttaa gcgcaccata cctggaagat gcagagatcg actttgttac
     2041 cgaccagttg ggttctcagt taacgctgaa agccccgaac gccaaaatgc gtaaagtggc
     2101 agacgatgca ccgctgatgg agcgcgtgga gtatatgctg cagtcgcaga tcaacccaca
     2161 gcttgctggt cacggtggtc gcgtttcgct gatggaaatc accgaagacg gttacgccat
     2221 tctgcaattt ggcggcggct gtaacggttg ttccatggtc gatgtgacgc tgaaagaagg
     2281 gatcgagaag cagctgctga acgaattccc ggagctgaaa ggtgtgcgcg atctcaccga
     2341 acaccagcgc ggcgaacact cctactacta agttatcccc tcttttgtag attgcccgat
     2401 gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt gccagacgtt ggcacagcat
     2461 tgctttggca aatccctccc ttcgtttccc cgcgataata tgaccaacct ctcataattt
     2521 aaatttaccc cgctctggtg attctcaaac gccaaatgtt acccgtatca ttcacatggg
     2581 taccaaacat actcctgaca tctgactaca ataattagtt ttagtgggta tcagtcgtgg
     2641 tgccgcaata tctctgttcc cgattgggat aattagagtt tgtcgtcaga aaattgacgt
     2701 tacccataac aaatgaaagg ccaggtaaat catgccatta gtcattgttg ctatcggtgt
     2761 aatcttgttg ttgctcctga tgatccgctt caaaatgaac ggcttcatcg ctctcgtcct
     2821 cgtggcgctt gctgttggat taatgcaagg aatgccgctg gataaagtta ttggctccat
     2881 caaagccggt gtcggcggga cgctcggtag ccttgccctg atcatgggtt ttggcgcaat
     2941 gctgggcaaa atgctggcag actgcggtgg cgcacaacgt atcgccacca cgctgattgc
     3001 caaatttggt aaaaagcaca tccagtgggc ggtggtgtta accggtttta ccgttggttt
     3061 tgccctgttc tatgaagtgg gcttcgtgct gatgctgccg ctggtgttta ccatcgcggc
     3121 ttccgcgaat attccattgc tgtatgttgg tgtaccaatg gcggctgcac tgtctgtgac
     3181 ccacggcttc ctgccaccgc atccgggccc gactgcgatt gccaccattt tcaatgccga
     3241 tatgggtaaa accctgctgt acggtactat tctggcaatc ccgaccgtga ttctcgccgg
     3301 tccggtttac gctcgcgtgt tgaaaggtat cgataagcca atcccggaag gtctctacag
     3361 cgcgaaaacc ttcagcgaag aagagatgcc gagctttggc gtcagcgtct ggacctctct
     3421 ggtgccggtt gtgctgatgg cgatgcgtgc gattgccgaa atgatcctgc cgaaaggtca
     3481 cgctttcctg ccggtagcgg agttcctcgg tgacccggta atggcaacgc tgattgccgt
     3541 gctgattgcg atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc agattaacga
     3601 cacgctggtt tcttccatca aaatcattgc gatgatgctg ttgatcatcg gtggtggcgg
     3661 tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa tacattgctt ccatgatgca
     3721 cgaaaccaac atttctccgc tgctgatggc ctggtcgatt gctgccgtac tgcgtatcgc
     3781 gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt atcgcggcac cgctgattgc
     3841 aacgacgggt gttagcccag agctgatggt tattgcggtg ggttccggta gtgtgatttt
     3901 ctctcatgtg aacgatccgg gcttctggct gttcaaagag tactttaacc tgactatcgg
     3961 cgagaccatc aaatcctggt cgatgctgga aaccattatc tcggtgtgcg gtctggtagg
     4021 ctgtctgctg ctgaatatgg tgatttgata acactgactg ccggatgcat cgcatccgac
     4081 atcaaccgca ctctacttct tcttcgctgc agctctgcgc cgtctgtcca aatccttcag
     4141 caacttgttc acgccatcat cggcaaacat cgactcaagc gttgcggaaa gcttgcgtcg
     4201 ccagttttta tactggtaac tggtgccagg aatattcacc ggttcggcca tatccagcca
     4261 gtcttccggc tgtagtccta acagagcact gttactgtcg gcaatgtagc gctgcaaacc
     4321 acggttcagc gtcggcgtca tcgacatcaa cgatgcctta tgcccggcac gttttggcag
     4381 acaaccatat ttatgcagtg catccagcag cccttgcttc gccagttcgc gatcctgata
     4441 cagaccgcgc agtaccactt catccggata cagccccagg gttttgccca gcgttagatc
     4501 cccgctctcc cagtaaccac gcagcgttgg caggtcatgt gtcgccgcaa ccgccatcga
     4561 ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg tggtcgtttt cgaaatagag
     4621 cactttgtaa gagtacacac cgctgctgcg cagcttacca acaatctcta ccggtacggt
     4681 accgagatct tcaccaatca ccatacagcg atgacgttta ctttcgagtg ccagaatcga
     4741 gagcagatca tccaccggat agtgaacata cgcgccctga tctgccgtct cgccatacgg
     4801 tatccaccac aaacgcagca tcgacatcac atggtcaatt cgtaatgcgc cgcagttttg
     4861 catattggca cgcaacagct cgataaacgg ttcataggca cgcgcggtga tgatatgcgg
     4921 gtccattggc ggtaatcccc agttctgccc caacgggccg aggatatccg gcggcgcgcc
     4981 aaccgatgct ttcaggcaat atagttcacg gtcacaccag gtttccgccc caccttccgc
     5041 tacgccaacc gccagatcac gatacaagcc aatcggcatt tcatagccct ggcttatctc
     5101 ccagcaggcg gcaaactggc tgtaagccag ccactgcaac cagagataaa aatcgacgtc
     5161 atcacgatgt tcttcgcaga actgacgcac ttctggtgaa tccacgttct tatacatctc
     5221 tggccatgca ggccagcccc agcgcatttc gtcctctttc acttgctggg catgtagcgc
     5281 atcaaaggca gcctgccaga acaggctgtc gccctgctct gcaacaaact ggcgaaacgc
     5341 ggccatctgc tcatcatcac gttgcgcgaa acctttccac gccattcgta atgctgtcat
     5401 ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca tcgcgcgcct gttgcagcgt
     5461 ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct tcgctaagat ggaaatcttc
     5521 aacggcgtta acgtcgatat aaatcacatt cagccaacgg cgagaagacg ggctgtatgg
     5581 gctggcgctc tccggatttg ccggatagag cgcatgaatc gggttcaggc caatgaacga
     5641 cccgccacgt tttgccacat ccaccagcat cgctttgaga tcgccaaaat ccccaatacc
     5701 ccagtttttt tccgatcgca gcgtataaag ctgaacgcag gcaccccaca gcttttgttt
     5761 attcagcaaa gcctgcggtt cgtaacaacg tttcggggcg acaatcaccc ggcaatgcgc
     5821 gcgctggtcg tcctgggtga gtgttagcgt gtgataacct tccggcagct tcgtcggtag
     5881 attgaacgct ttgccccccg ttacatggcc tttgtactgc gttccttctt cggtggtcag
     5941 ctgccagcta tattcgccgc tgccctccac caccatcggc atttttttgc cgctggtata
     6001 aaccatgaca ttcgggactg gcgttaccgc cactttcgtg gcggtacgtt gatgcatcgc
     6061 gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc ggtttaccgt gggcattgat
     6121 gtaattgggg ctaatccccg ccgccagcgc ggcattatcc agacgtttgc tttccatcga
     6181 gcttccttag cgttttgcct gccagatacg agcctgataa tcgcgaatag agcgatccga
     6241 gctaaacata ccgcagcggg cggtattgag gatcgccgcg cgagtccagg cctcctggtc
     6301 gcggtacagc acatccacct gcttttgtgc ctcaacatag gctgcgaaat ccgccatcac
     6361 cagatacgga tcgccgccct gtttgccgat actgtgcagc atctggtcga aggcatgctt
     6421 atcgccgtcg ctgtatttac cgctttccag ctctttcaat actgcgtcca gcaccttatc
     6481 tttcttccgc catttcaccg ggtcgtagcc tttggccaga atagccttca cttgttccac
     6541 ggtatgacca aaaataaaga tattttcttc accgactttc tcggcgattt caacgttcgc
     6601 accatccagc gtaccgacag taagcgcacc attgagcgcc agtttcatat tgccggtacc
     6661 ggaagcttct ttacctgcag tcgaaatttg ttcggagata tccgccgccg ggatcagttt
     6721 ttccgccgcc gaaacgcaat aatccggcag gaacaccacc ttcaacttat cgccaaccag
     6781 tggatcgttg ttgatcacgt cagccacttt gttgatcgca aagataatat tcttcgccag
     6841 gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg cgcggtacgc gatcagcctg
     6901 caggttttca cgaatttctt tgtacaacgc cagaatatgc agcagattca ggtgctggcg
     6961 tttgtactca tgcagacgtt tgatctgaat atcgaaaatc gcctgtggat tgatctcaat
     7021 accggtacga actttcacaa actccgccag acagacttta ttcgcctgct tgatctcgcg
     7081 atattgctga cggaatttcg catcatcagc gaatttttcc agattgatca gctgatcgag
     7141 atcgttagcc cactcttttt gcagtgattt atccaacaga gccgccagtg cagggttgca
     7201 ctgtttgatc cagcgacgtg gggtaatacc gttggtaacg ttatggaatt tgttcggcca
     7261 tagctggtga tattccggga acagatcttt caccaccaga tccgagtgca gcgccgcaac
     7321 accgttcacc gcgaaaccgc caaccacaca caggttcgcc atatgcactt gtttatcgtg
     7381 caccaccgcc agtttggccc acactttttc atcgcccggc caggttttct ccaccagcgt
     7441 tttaaagcga gtattaattt cgttaataat ctgcatatgg cgcggcagta agcctttcac
     7501 cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg gtatggttgg tgtaagcgaa
     7561 agttttgctg gtgatggccc aggcgtcatc ccagctcatc tggtgctcat cggtcagcac
     7621 gcgcagcagt tctggaatcg cgatagttgg atgggtatcg ttcagctgaa taacttcgta
     7681 atccgccagt tcgtgcagtt tacgccccgc cagatgatgg cgacgcaaaa tatccgctac
     7741 cgaacaggca cactggaagt attgctgcat caggcgcagc tttttaccgg cagtatggtt
     7801 gtcgtttgga tagagaactt tggtcagttt ttccgcattg atgccttgct gttcggcgcg
     7861 caggaaatca ccgtcgttaa atttagttag atcaaacgga tgcgcatgcg tcgcctgcca
     7921 cagacgcagc ggctgcgcca cgccattacg atagccgaca acggggagat cccacgcttg
     7981 accggtaatg gtaaactccg gctcccagcg tccgtctttc atcactttac cgccaatccc
     8041 tacctgcaca tccagtgctt cgttgtggcg gaaccacggg tagttactgc gatgccagtc
     8101 atccggcgct tcaacctgtt tgccatcgac aaaagactgg cggaacaaac catattgata
     8161 gttcagaccg taacccgtcg cagactgacc gacagttgcc attgagtcga ggaagcacgc
     8221 cgccagacgt cccagaccac cgttacccag cgccgggtcg atctcttctt ccagcaggtc
     8281 agtcaggttg atgtcataag ccttcaacga atcctgtaca tcctgatacc agccgagatt
     8341 caacaggttg ttgcccgtca gacgaccaat caaaaactcc attgagatgt agttaacatg
     8401 tcgctgattc gccaccggtt tggcgaatgg ctgagcacgc agcatttcgg ccagtgcttc
     8461 actcactgcc agccaccact ggcgaggagt catttcagcc gcagaattta agccataacg
     8521 ctgccactga cgtgaaagca cttcctgaaa ttgcttatcg ttaaaaatag gttgtgacat
     8581 aggagttcca cttttcttag attttcaaca caacgttatc gctagtttgc caggctcgat
     8641 gttgaccttc ctcatcctgc gggggattag gcagggagga gttgcgggga tgagcaagga
     8701 aatgtgatct cgaccactta aagctagtgc aaaccacagg attagcagca aatcaatgcc
     8761 atacagcgca gaaaatctgt atctaagtgc aaaaaatggc cgttgcgtat tttcaaaaag
     8821 cggaaggtaa ctctataaat taagtaaagg agtgaaacag tttcacaagt aaaatatcca
     8881 gtgtgctcca tctcattctt aatagattta ttaagatcat ctttttagat ggcactttca
     8941 tcaggaatga agaagaaacc cttgcttaaa tgaatctgat gaacataagg gaaaccagta
     9001 ttcacgctgg atcagcgtcg ttttaggtga gttgttaata aagatttgga attgtgacac
     9061 agtgcaaatt cagacacata aaaaaacgtc atcgcttgca ttagaaaggt ttctggccga
     9121 ccttataacc attaattacg aagcgcaaaa aaaataatat ttcctcattt tccacagtga
     9181 agtgattaac tatgctgatt ccgtcaaaac taagtcgtcc ggttcgactc gaccataccg
     9241 tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa caacttccgg ctggcgctga
     9301 tcacgagtcc tgcgggctac ggaaagacca ccctcatttc ccagtgggcg gcaggcaaaa
     9361 acgatatcgg ctggtactcg ctggatgaag gtgataacca gcaagagcgt ttcgccagct
     9421 atctcattgc cgccgtgcag caggcaacca acggtcactg tgcgatatgt gagacgatgg
     9481 agcaaaaacg gcaatatgcc agcctgacgt cactcttcgc ccagcttttc attgagctgg
     9541 cggaatggca tagcccactt tatctggtca tcgatgacta tcatctgatc actaatccag
     9601 tgatccacga gtcaatgcgc ttctttattc gccatcaacc agaaaatctc accctggtgg
     9661 tgttgtcacg caaccttccg caactgggca ttgccaatct gcgtgttcgt gaccaactgc
     9721 tggaaattgg cagtcagcaa ctggcattta cccatcagga agcgaagcag ttttttgatt
     9781 gccgtctgtc atcgccgatt gaagccgcag aaagcagtcg gatttgcgat gacgtttccg
     9841 gttgggcgac ggcactatag ctaatcgccc tctccgcccg gcagaatacc cactcagccc
     9901 ataagtcggc acgccgcctg gcgggaatca atgccagcca tctttcggat tatctggtcg
     9961 atgaggtttt ggataacgtc gatctcgcaa cgcgccattt tctgttgaaa agcgccattt
    10021 tgcgctcaat gaacgatgcc ctcatcaccc gtgtgaccgg cgaagaaaac gggcaaatgc
    10081 gtctcgaaga gattgagcgt caggggctgt ttttacagcg gatggatgat accggcgagt
    10141 ggttctgcta tcacccgctg tttggcaact tcctgcgcca gcgctgccag tgggaactgg
    10201 cggcggagct gccggaaatc caccgtgccg ccgcagaaag ctggatggcc cagggatttc
    10261 ccagcgaagc gattcatcat gcgctggcgg caggcgatgc gctgatgctg cgcgatattc
    10321 tgcttaatca cgcctggagt ttgttcaacc atagcgaact gtcgctgctg gaagagtcgc
    10381 ttaaggccct gccgtgggac agtttgctgg aaaatccgca gttagtgtta ttgcaggcgt
    10441 ggctaatgca aagccaacat cgctatggcg aagttaacac cctgctagcc cgtgctgaac
    10501 atgaaatcaa ggacatcaga gaaggcacca tgcatgcaga atttaacgct ctgcgcgccc
    10561 aggtggcgat taacgatggt aatcaggatg aagcggaacg gctggcaaaa ctggcactgg
    10621 aagagctgcc gccgggctgg ttctatagcc gcattgtggc aacctcggtg ctgggtgaag
    10681 tgctgcactg caaaggcgaa ttgacccgct cactggcgct agtgcagcaa accgaacaga
    10741 tggcacgcca gcacgacgtc tggcactacg ccttatggag tttaattcag caaagtgaaa
    10801 ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac gcaggaaaaa gcgttccagc
    10861 tgatcaacga gcagcatctg gaacagctgc caatgcatga gtttctggtg cgcatccgag
    10921 cacaactgtt atgggcctgg gcacggctgg atgaagccga agcgtcggcg cgtagcggga
    10981 ttgaagtctt gtcgtcttat cagccacagc aacagcttca gtgcctggca atgttgattc
    11041 aatgctcgct ggcccgtggt gatttagata acgcccgtag ccagttgaac cgtctggaaa
    11101 acctgctggg gaatggcaaa tatcacagcg actggatctc taacgccaac aaagtccggg
    11161 tgatttactg gcaaatgacc ggcgataaag ccgccgctgc caactggttg cgtcatacgg
    11221 ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca atggcgcaac attgcccgcg
    11281 cacaaatctt gctgggcgag tttgaacctg cggaaattgt tctcgaagaa ctcaatgaaa
    11341 atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa cctgttgctg cttaatcaac
    11401 tgtactggca ggccggacgt aaaagtgacg cccagcgcgt gttgctggac gcattaaaac
    11461 tggcgaatcg caccggattt atcagtcatt ttgtcatcga aggcgaagcg atggcgcaac
    11521 aactgcgcca gctgattcaa cttaatacgc tgccggaact ggaacagcat cgcgcgcagc
    11581 gtattctgcg agaaatcaat caacatcatc ggcataaatt cgcccatttc gatgagaatt
    11641 tcgttgaacg tctgctaaat catccggaag tgccagaact aatccgcacc agcccgttga
    11701 cccaacgtga atggcaggtc ctggggctga tctactccgg ttacagcaat gaacaaattg
    11761 cgggcgaact ggaagtggcg gcaaccacca tcaagacgca tatccgcaat ctgtaccaaa
    11821 aactcggcgt cgcccaccgt caggctgccg tgcagcacgc gcagaaactg ctgaagatga
    11881 tgggttatgg ggtgtgagtt tagccggata atgcattgga tccggtttac atctttacat
    11941 cattcaatac tcacccgcgt tacgccatct gtttctatca aaccaaaccg caccggcaag
    12001 aaacgctcca ccaccgcgat attggtcagc agatggcatg aaggatgggc gaccgtaaat
    12061 tctcccgcgc ccgccagcgc catcggcagt accagttggt cggcgagata ttcccccacc
    12121 gccgctgggc ttgccaggta gcgtttcacc tctttcacta actgcgcggc taccacctcg
    12181 gcactgacgc gcttttcacc gacgacgaaa aagcgttcag tgatattttc actttcgact
    12241 tccagcgaga cggtattacc cggcccctgg tcgcgcggca ggttatgaat attctgttca
    12301 tgcagggaaa aaatccccgc cagtgtagcg atttcacgct cagcaacatg gcgcggcaca
    12361 ccagctaata gaacttctcc acgcatctgc acaatgttcc cgcgctcgcc aagttgcaag
    12421 gtgctaaacg atgccacggg cgagacttcc gttgccacca caccgcctcc ggcaggataa
    12481 aaaccgtgac gtaacagcgt ggtttgctga tgaattccta ttttcgccag cagcggctcc
    12541 agcacccggc ggataaaatc cgcaggcggc gccgacgggt tatcggtgcc gccgctcact
    12601 tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca gcaccgtttg cagcaccagc
    12661 gtacaacttc cggcgctacc gatagcaaag cggtaatcgc cgccgcgcac ggtgccaggc
    12721 cggaagacca gacgctgcga tcccagctcc gcgccttcca ccgttgccct acaaatttcc
    12781 gcagccgctt ttaccgcggt cagatgctgg cgcagcagcc ccggtttcgc ccgtccggct
    12841 cgaatgccag tgatggtaaa tggctggccg gttatcatcg acaggctcag cgccgagcgc
    12901 aggatctgcc cgccgccttc gccctgtgcg ccatccagcg caatcatcct tttcatcatt
    12961 tatcctttta cgcacaccac ctgacgcagg gtgtagataa cttccaccag atcgctttgt
    13021 gccgccatca ccgcatcaat atctttatac gccatcggga tttcgtcgat cacttcggca
    13081 tctttacggc attccacatg cgcggtggca cgaatttggt cttccacgct gaacagtttt
    13141 ttcgctttag ttcggctcat tacccgcccg gcaccgtggc tgcacgaaca gaacgactct
    13201 tcatttccca gcccacggac gataaagctt tttgctccca tcgaaccggg aataattcca
    13261 tattgaccag cacgcgcaga caccgcacct ttacgcgtca cgtagatctc ttcaccaaag
    13321 agctgttctt tttgcacata gttgtggtga cagttgatct cttccatcgc cagcgtttgt
    13381 ggctgtctga ccgttttctg cgtaatgctc tgcaacgccg ttaccacatt ttccatcatc
    13441 gcatcgcggt taaggctggc gaaaagctgc gcccaggcca cggctcttag gtaatcatca
    13501 aagtattccg taccttccat aaagtacgcc agatcacgcg acggcaacgt ctcaagttgg
    13561 tcctgcatct ctttttgcgc cagatcgata aagtaagtcc cgatggcgtt accaattccg
    13621 cgtgaaccgg agtgcagcat aatccacacc tgctccgact catcaaggca gatttcaata
    13681 aagtggttac ccgttcccag cgttcccagg tgtttatagt tgttagtgtt aaggaaacgc
    13741 ggatattttt gcgttaacca ctgataaccg gcctccagct cagcccattt cgcatcgacg
    13801 ttaaaaggcg ggttttccca tgcgccttta tcacgtttac aacgcccggt agtacgcccg
    13861 tgtggtacgg ccgtttcaat cgcttgacgc agctccgcca ggttttcagg caggtctgcc
    13921 gccgttaacg cggtacgcag cgcgttcatt ccacagccaa tatccacgcc caccgccgcc
    13981 ggaataatcg cccctttggt cgggatcacg ctaccaatgg tggaaccttt acccaggtgt
    14041 acatcaggca ttaccgcaat atgtttgaaa ataaacggca tcttcgccgt attaataagt
    14101 tgctgacgcg catcggcttc taccggcacg cctttggtcc acatttttac cggggcattt
    14161 tcagtggtca gtaattcgta attcattttg tttttctctt ttcgttgttt gcggtcctga
    14221 taattgcaac cgtcgtgcca gaaaattaaa taagcagatt aatttattgt ttctaaaaag
    14281 attatatctt tatatccgta aacggagatt tcccgcaaag ccgatttacc gataatgaaa
    14341 tatcgccttt tataaggata tctaagatgc gtaaaacagt ggcttttggc tttgtcggta
    14401 ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc aaaatggcgt ccgtcactct
    14461 gtatatacca gcaagaatcg ttggtcatcg atcgactgga attattgcac gatgcccgct
    14521 cgcgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca gaaacagaag
    14581 tggtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt tacgcctgcc
    14641 tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat ttaattcaca
    14701 tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa gcgcgttatc
    14761 tacccgcccg gttgatacaa tcttcaactc cgcgcaaaaa agaacagccg cgcggcccag
    14821 gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc agccgctttg
    14881 ccgaggaacg gcagcaaacg ctcgatttcc tcaaatccgg tattgccacg cgtaaccccc
    14941 acttcaaccg catgattgag cagatcgaaa aagtggcgat taaatcccgc gcgccgattc
    15001 tgcttaacgg cccgaccggc gcaggtaagt catttctggc gcgacgcatc ttcgagttaa
    15061 aacaggcacg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc accctgcgcg
    15121 gcgataccgc gatgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc ggggcgcggg
    15181 aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt gatgagattg
    15241 gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc tattgaagag aaaacctttt
    15301 acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc ggaacggtgc
    15361 gcgatttgcg ccagctggtt gccgaaggca aattccgcga agatctgtac gcgcggatca
    15421 atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt gaaccgaacc
    15481 tggattatga agtggagcgc cacgccacac tcactggtga cagcgtgcgt tttaacaccg
    15541 aagcgcggcg tgcctggctg gcctttgcaa catctcccca ggcaacatgg cgcggtaact
    15601 ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc ggacgcatca
    15661 ctctggacgt ggtggaagat gagataaacc gtctgcgcta taactggcag gagagccgcc
    15721 cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc ttcgaccgta
    15781 tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct gctgccggac
    15841 gacagctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg gatcggctac
    15901 gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag cacagctcca
    15961 gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg gcgtcggtgt
    16021 agacggcatc aaccatgctg atgctcccca tattgaccat cgcattacgg ccaaattttg
    16081 aatggtcaac caccagcata acgtggcgcg agttctcaat aatggcgcgc ttggtacgaa
    16141 cttcgtggta atcgaactcc agcagcgagc cgtcgctgtc gatgccgctg atccccagaa
    16201 tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca atgatcccgc
    16261 catcgcggct acgtaattcg ccaccggcga gaataatgcg gaaatcttct tttaccatca
    16321 acgtgttagc aacgttgaga ttgttggtga caatgcgcaa attgctgtga ttgagcagcg
    16381 cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag ccattgggga
    16441 tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg gctttacgat
    16501 cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc gccgccatga tggcgcagga
    16561 tcaggttttg ctctgccagc tcgttgaggt cgcggcgaat agtctgcggg ctgacggaga
    16621 agtgctccac cagttcctcg gtactgacat aaccctgctg tttaaccagt tcgataatac
    16681 cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg ttttcgcgca
    16741 ttgagcgaat caacaaaagc catcgctaaa cccacggcta atccggcgat gtgtgctccg
    16801 ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat cagcgcaaag
    16861 ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc acgtagccag
    16921 acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg cccgctgaat
    16981 ttttgctgca tatagccgct taacagggcg ctgataagcg taatgacaat tagcttaccg
    17041 ctaccgaggc gtttttccac cgcgccgccg agataccacc accaaagcag gttaaagagg
    17101 atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa ctcaaatttc
    17161 agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc gagaatttgc
    17221 atggcaataa acaccaccac gcaggcgatc atcatcaccc aggtcaccgg acctgcgcgt
    17281 tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc ggtatggcct
    17341 gccagccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa acgcgccagc
    17401 tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct ttggttatgt
    17461 tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc ctgcgccacg
    17521 cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc cacacaaaag
    17581 gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgt cgctgccagg
    17641 cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc agatactgcg
    17701 ccgccccttt gctgctattg ccgtgataac acatcaccat caccggagtg tcaaagtcgt
    17761 tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc accgcatgcc
    17821 ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt tcctgcaact
    17881 tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc tctttcttta
    17941 caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa acgccataat
    18001 gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat tctttgctaa
    18061 tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca gcatggaaac
    18121 caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg cagacgccgc
    18181 tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg cgacctcttc
    18241 cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg aattccgcct
    18301 ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc atatcgcctt
    18361 cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga tgattcgcat
    18421 tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat caactggttt
    18481 gcgttttggc gcaaattcag tgttaaaacc ggaaattaag cgcggattcg aatattctga
    18541 ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg tgcgtaaagg
    18601 cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg gcctgtggat
    18661 tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc gcggcctggt
    18721 taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc tgccttcgcc
    18781 ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgcgtgc atacccaaaa
    18841 gcaggcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc cgtggatgga
    18901 cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggtgatccga aagcagtgaa
    18961 gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact ttaaaaagca
    19021 gctaagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt gtgatgatga
    19081 gtccgactcg ccgcaagcca ttacccgtga ttacaccctt gatatccatg atgaaaacgg
    19141 caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa aactggcaga
    19201 acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt ggacgaaaga
    19261 gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgccg ctcgcctgcg
    19321 ccgccgttac ccgttcctga ctgaatcgct ggcacgtcac tacacgcgca cttacggcag
    19381 caacagcgag ctgctgctcg gcaatgcggg aacggtaagc gatctcgggg aagatttcgg
    19441 tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg tccgccgcac
    19501 cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg atcaacaatc
    19561 tcgtgtgagt cagtggct
//
LOCUS       DAJTYM010000078        19341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTYM010000078 DAJTYM010000000
VERSION     DAJTYM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(145..1578)
                     /gene="hldE"
                     /locus_tag="OO575_002799"
     CDS             complement(145..1578)
                     /gene="hldE"
                     /locus_tag="OO575_002799"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6665699.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1626..4466)
                     /gene="glnE"
                     /locus_tag="OO575_002800"
     CDS             complement(1626..4466)
                     /gene="glnE"
                     /locus_tag="OO575_002800"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6665700.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4489..5790)
                     /gene="ygiF"
                     /locus_tag="OO575_002801"
     CDS             complement(4489..5790)
                     /gene="ygiF"
                     /locus_tag="OO575_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6665701.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6032..6652
                     /locus_tag="OO575_002802"
     CDS             6032..6652
                     /locus_tag="OO575_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6665702.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6716..7954
                     /gene="cca"
                     /locus_tag="OO575_002803"
     CDS             6716..7954
                     /gene="cca"
                     /locus_tag="OO575_002803"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6665703.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8117..8938)
                     /gene="bacA"
                     /locus_tag="OO575_002804"
     CDS             complement(8117..8938)
                     /gene="bacA"
                     /locus_tag="OO575_002804"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6665704.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9029..9397)
                     /gene="folB"
                     /locus_tag="OO575_002805"
     CDS             complement(9029..9397)
                     /gene="folB"
                     /locus_tag="OO575_002805"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6665705.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9502..10119
                     /gene="plsY"
                     /locus_tag="OO575_002806"
     CDS             9502..10119
                     /gene="plsY"
                     /locus_tag="OO575_002806"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6665706.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10132..11063)
                     /gene="ttdR"
                     /locus_tag="OO575_002807"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10132..11063)
                     /gene="ttdR"
                     /locus_tag="OO575_002807"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11270..12113
                     /gene="ttdA"
                     /locus_tag="OO575_002808"
                     /pseudo
     CDS             11270..12113
                     /gene="ttdA"
                     /locus_tag="OO575_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12110..12715
                     /gene="ttdB"
                     /locus_tag="OO575_002809"
     CDS             12110..12715
                     /gene="ttdB"
                     /locus_tag="OO575_002809"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6665707.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12763..14226
                     /gene="ttdT"
                     /locus_tag="OO575_002810"
     CDS             12763..14226
                     /gene="ttdT"
                     /locus_tag="OO575_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6665708.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14269..15282)
                     /gene="tsaD"
                     /locus_tag="OO575_002811"
     CDS             complement(14269..15282)
                     /gene="tsaD"
                     /locus_tag="OO575_002811"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6665709.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15520..15735
                     /gene="rpsU"
                     /locus_tag="OO575_002812"
     CDS             15520..15735
                     /gene="rpsU"
                     /locus_tag="OO575_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6665710.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15846..17591
                     /gene="dnaG"
                     /locus_tag="OO575_002813"
     CDS             15846..17591
                     /gene="dnaG"
                     /locus_tag="OO575_002813"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6665711.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17786..>19341
                     /gene="rpoD"
                     /locus_tag="OO575_002814"
     CDS             17786..>19341
                     /gene="rpoD"
                     /locus_tag="OO575_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6665712.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHL"
BASE COUNT         4606 a         5402 c         4928 g         4405 t
ORIGIN      
        1 actgttaagt ttcccgactt actaacaact gatcagaggg gggagaaatc ctcccttacc
       61 cttgttcctt tactttaggt tgaaaaaaca acagcgtcaa taggcctgcc tgctacgaag
      121 cgagatctgt gaaccgcttt ccggttagcc ttttttatcc tgttggatct tcttgatgat
      181 gttagtggtc gagcaaccgt cttcgaagtt gagcaccagc acttcgccgc cgttggccca
      241 gacttcttta ctcccggcga tctcttctgg tttatagtcg ccgcccttca ccagtaaatc
      301 tggcaggatc ccggcgatca agcgctgcgg cgtgtcttct tcaaacgaca ccacccagtc
      361 gaccgcttcc agtgcgccca gcacaatcat acgctgttcg agcgggttaa cggggcgaga
      421 atcccctttc agccgtttgg tggaggcatc gctgttcaca gcgacaatca agcggtcacc
      481 cagtttgcgg gcatttgcca gatagaagac gtgtccggcg tgcaggatgt caaagacgcc
      541 attggtcatc accacttttt caccgcgttt acgcgctgcc gctacggcca gcttcagttc
      601 ctcttcggtc atcacgccaa agccggtatc tgcgcgccca cgcactgcat tttccagctc
      661 gatcggcgaa accgtggagg ttcccagttt gccgaccacc acgccagccg ccgcattggc
      721 aaagaagcag gcttcttcca gcgaattacc cgctgccagc gttgccgcca ggacgccaat
      781 caccgtgtcg cccgcaccgg taacgtcata cacttcctgc gcctgggttg gcatatgcag
      841 cggcgcttta cccggttgca gcagcgacat accctgttcg gaacgggtca ctaacagagc
      901 cgagagttcg taatcggcaa tcagtttcat gccgcgctca acaatctctt cttcggtctt
      961 acatttaccg acaacagctt caaattccga gagattcggt gtcagcagcg tagcgccgcg
     1021 gtagcgctca aaatcggtac cttttggatc aatcagcacc ggaacgcccg ctttacgcgc
     1081 cagttggatc atctgctgta cgcttgccag cgcacctttg gcgtagtcag aaagcaccag
     1141 cgcgccaatc gaactcagcg cctgattaat ccgctcgtgc agcggctgcg gatcaacacc
     1201 ttcgaaacct tcttcaaaat ccagacggat cagctgttgg ttgcgggaaa gtacccgtaa
     1261 tttggtgatg gtcggatgcg tcggtacaga aacgaagtcg catttgacgt tgacgtcggc
     1321 cagagattta ctcagcgcgc gcgctgcatc gtcaatgccc gtcaacccga ccaggcgtgc
     1381 attagcaccg agagaagcga tattcatcgc cacgttagcc gcgccgcccg gacgttcttc
     1441 gatggtattc actttaacca cgggcaccgg cgcttccggc gagatacgac tggtggggcc
     1501 gtaccagtaa cgatccagca tcacatcacc aaccaccatc actcctgcac gttcaaactc
     1561 tggcagcgtt actttcattc ctgtctcctg agagattcaa aatttgcgcg cgataatacc
     1621 atacttcatt cttccaccag ccacttctgc cagcttgccc gcaccagatc acgctctgcg
     1681 gtgaagcaat cctccgacac atggcccggc aattcctgta atgccagatg atgaagttca
     1741 tcgcgcagcg tcgtgtaagc acgggtcagc gccatcgctt cctgctcttc cataatgtcg
     1801 ttttgcgcca gcagctccag aatacgcacg ttgtctgacc agcgcgttaa cttcggtttt
     1861 tcatgagcgt agcgcaacac cagatattgg gtaataaatt cgatatcggt aattccgcct
     1921 tcatcggctt tgatatcaaa gcgattacga tgtttattgc cgagatgagc gcgcattttc
     1981 tcgcgcattt cccgcacttc cgtttgcaga gttttacctt ctcgcggcag tgtcataatc
     2041 tcacgacgca ccgcgtcaaa gtgcgcagtg agctgcggat cgccgtacac tacacgcgca
     2101 cgcaccagcg cctgatgttc ccacgtccag gcctcgtttt tctgataatc ggcaaatgct
     2161 tctgcggatg tcaccagcat tcccgccgcc ccggacggac gcagtcgagc atccacttca
     2221 tacaaaatgc cggaagaggt acgcgtactg aacagatgca taatgcgttg cgccagacgc
     2281 agataaaact gccgcccgtc gatttcccgc tcgccgtcag tcatcgcatc cattgggcaa
     2341 tcatggagga agataaggtc aagatcggaa ctgtagccta actcccagcc gcccagcttg
     2401 ccgtagccga ccaccgcaaa accgcgccct tcacgttcgt tgaggtgatt tggcttaccg
     2461 tagcgggcca ccatttgacc ccacgcctgc tgaacgacgg catctatcat agcttctgcc
     2521 agccaggtta agtgatcgct cactttcatt accggtagcg taccggcgat atccgctgcc
     2581 gcaatgcgta acagctgcgc ctgtttgaac tgacgcagcg cctcaagctg ttgttcttcg
     2641 tcatcttccg gcacgcgcag caaatactgg cgcagttcat cacggtaggc atcggtcgcc
     2701 gtcggctgat aaagggtatt tggatcgagc aattcatcca gcaataatgg ataacgcgct
     2761 agctggctgg caatcatcgg cgatgcggca cacagagaaa tcaaatgttt aagcgccgca
     2821 gggaattcac tgagcaattc taaataggtt gtgcgggtaa caatccccac cagcaaggcg
     2881 gtaatgcgcg acagcgtgac agcagcgtct tcacgcgcac agacatcact tagcagatgt
     2941 ggcatcagat ggtcgagcac ctgacgtcct cgcggcccga tagtacgctt atccagctct
     3001 ttgcggaaat cagcaatcat cgttaggacc tgtttgcgat catcctcgct aagatgcgcc
     3061 agcactggcg tagtgtcatc ttcctgcaac gcatcctgcc acagctcacg ccactgttcc
     3121 gacagcgact cttcctgagt ttcactttcg tcgtcgccaa tcaattcatt aaacacccgg
     3181 cgcacattgg tcatatgtgc ggtcagcgcc ccggtcagtt gcggccagtc agcaaagtcc
     3241 atcgcccacg ccagccgcgc acggttaagc tcatcagaag gaagcgtttg ggtttgttcg
     3301 tcgttaatgc tttgcagcag gttttccaga cgccgcagga acagatatgc cactcgcaat
     3361 tgttcagcat cgttttcaga aagcagatgc agcgcggcaa tggcgctgag cgttggcagt
     3421 aaagagcgcg actgcagcaa cggttcgcgc ccaccgcgaa tgagctggaa cacctgaacg
     3481 ataaattcaa tttcgcgaat gccgcctgcg ccgagtttga tattgtcggt caaaccacgt
     3541 cgacgcactt cacgggcaat catccctttc atgttgcgca gcgactgaat cacgctgaaa
     3601 tcgatgtaac gacggaaaac aaacgggcgc agcatcgcac gcaactcgtt agcatagacg
     3661 ccttcgctat cgcccataat ccgcgctttg accatcgcgt aacgctccca gtcgcgtccc
     3721 tgctcctggt aataatcctc caacgcggca aagctcagca ccagcgggcc actttcgcca
     3781 aacggacgca gtcgcatatc cacgcgatag acgaagccat cctgcgttgg ttgatccagc
     3841 actttaatca gccgctgccc catgcgggta aaaaactgcg cgttatccag ttcccggcgt
     3901 ccgccctgcg tacaaccatg ttccggccag gcaaaaatca gatcgatatc agaggagaaa
     3961 ttcagttccc cgccgcccag cttacccatg cctaagatca gcagcggttg cgcttcgccc
     4021 tgcgcattgc acggcgttcc ccactcgcgg cagcaggcgt catacagcca gtcacgcgcc
     4081 gcaacaatca gcgtctccgc cagataactg agctgctgca atatgctctc ttcagtaacc
     4141 agtgccagcg tttgcgccca tgcgatgcgc accataatgc gccgccggaa tagccgcagc
     4201 tcgcgcatta acccggcttc gtcgctcaca ttactgagcg cctcctgcaa ccacacgctg
     4261 taatgttgcc attcgtcagc ctgtggcggt tggctttcca gttccgtcag ccactctgga
     4321 tgcgcaatca cgctgtcctg cacaaaatca ctgaaagtaa gtactgactt cgcctgtgca
     4381 ctaagtgatt cctcggctaa aagctctggc agccgctcaa caacggtctg ccagtactgc
     4441 tgtaacggtg aagagagcgg cttcataagc gattttatcc ttgcctgatt aacgttttcc
     4501 gctgtgcaac cagaacggtt cctgattgtt tgcctcatta cggaaatgtt caatttcaat
     4561 gcgttgcccg gtcgcaatag cgtgacgcag cccctgccag ttctccagcc aggcttgcgc
     4621 gacgacagga tcatagtaac ccgccagcaa cagtattgag tcaatatcac gcatcaggcg
     4681 tggcaactgg tcacggtagc gatcgcctaa cggctggcag aaaacgcttt tcagttcagc
     4741 ggcatggcgg gaaagatgga tatcggcaaa gcgtttgaag gagtcgctaa ttttgccctg
     4801 cgctttggca tctaaaaacg gctgccatgc tttgcttacc agccattcgg tcaacgccag
     4861 cttcgccatt gccgtttcgg tagagtagac cgccgtcacg gcagaaaccg cagaagcgat
     4921 ggtcgcttcg cattgagtca gcaaatcacg taagtgagta ctcgctttac gcggcacaat
     4981 gccaccgaac agcatcagtg tatgacggac taggctaatg gctgccagca cctgctcttt
     5041 cgccgcatcg ttgccgcgta cccacagttc ttcatgatac tgccattgcg ctaacaccag
     5101 ctcgagcgcc gcttccagcc cctgttccac atcggctttt gccgcaacat gcaaaatggt
     5161 ggtcggtttg atttcacgcg ccggattgcc ctgcgccaga tgatagccac gcgccgcttt
     5221 gctcaggctg ccctggcgta atccggtttg cgataccagt tggttcgcca gtttcagcac
     5281 cgcgcgcgtg tcgccgctaa gcagctccag ttccagctcg cagataggct cagcaaattc
     5341 acccgctttc acttccccct ggtcgagagc gatttcaatt cggctgccat cgatctctac
     5401 tagccatttt tcgcgataaa aatcggtgct aaacagcgac tgcacgcggg aagcgagatc
     5461 ggcaggcaat tcgccgttcg gccagatttc cgtcggtaac tgcgccaggt cgagcgtcgg
     5521 ttcgctcaac gccacgttat attccgggcg ctggtgtaag ccgcctgtca ctcttcctgc
     5581 aactttcatg gtcatctcat agcgaccgtt ttcgccacga atacgtaagc ccatatcgtg
     5641 cctacgcagc cagttatccg gcgtttcgta gtaaatattc agcaactgca cggggtcatg
     5701 gtgctcgccg cccagcgtat tgagatggtc acgcaacgcc tcaacggcac tgtgattaac
     5761 aataaacttt aattcgattt cctgagccat ggcctttact tatcggttat gtcacataag
     5821 tgacgatgaa cggcgaactt aatgcgatct tttttgtcag tagatagtat tttgcgccaa
     5881 attgccatgc aacgggcaat ttgacgggcg taaaagtttg aagcagtggc aagacctgac
     5941 acagaggatt ccgattgatg tcgaatgctt tgcgtagcga cactgatgcc actactatcg
     6001 ttccactttc catgacaata acgacagcct gatgccaaaa ttacgcctga tcggattaac
     6061 tttactcgca cttagcgcga ctgccgtctc acacgctgaa gaaacgcgct atgtttctga
     6121 cgaactgaat acctgggtcc gtagcggtcc gggagatcat tatcgcctcg tgggcacggt
     6181 taacgccggc gaggaagtga ccttattaca aactgacgcc aacaccaatt atgcccaggt
     6241 gaaagacagc tctggccgta ccgcctggat cccgttgaaa caacttagca ctgagccaag
     6301 cctgcgctcc cgtgtgccag atctggaaaa tcaggtcaaa accctgaccg ataaactcac
     6361 caatatcgat aacacctgga atcagcgcac cgcagaaatg cagcaaaaag tggcgcagag
     6421 cgacagcgtg atcaacgggt taaaagaaga aaatcagaaa ctgaaaaacg agctgattgt
     6481 cgcgcagaaa aaggtcgatg ccgccagcgt acagctggat gacaaacagc gcaccatcat
     6541 catgcaatgg tttatgtatg gcggtggcgt gctggggctt ggcttgctgc tcggtctggt
     6601 actgccgcac ctgatcccaa gccgcaaacg caaagatcgc tggatgaact aaatcgcctt
     6661 cttcgttgca ctgtcttatt attaggaatt gtgtatttga cgaagggatg atggcgtgaa
     6721 gatttatctg gtcggtggtg ctgttcggga tgcattgtta ggactatcgg tcaaagacag
     6781 agattgggtg gtggtcggca gtacgccaca ggagatgctc gacgcgggct accagcaggt
     6841 aggccgcgat tttcctgtgt ttctgcatcc gcaaacgcat gaagagtatg cgctggcacg
     6901 taccgaacgg aaatccggtt ccggttacac cggttttact tgctataccg caccggatgt
     6961 cacgctggaa gatgatctta agcgtcgcga tctgaccatt aatgcgctgg cccaggacga
     7021 taacggcgag attatcgacc cgtacaacgg tctgggcgat ctgcaaaatc gtctgttgcg
     7081 ccatgtttcc cccgcttttg gcgaagatcc gttacgcgta ctgcgcgtgg cgcgttttgc
     7141 tgcgcgttat gcccacctcg gttttcgtat tgccgatgaa actctgacgc tgatgcgcga
     7201 gatgacccat gcgggtgaac tggaacacct gacgcctgaa cgggtatgga aagagacgga
     7261 aagcgccctc accacccgca atccccaggt gttcttccag gtactgcgcg attgcggcgc
     7321 acttcgcgtt ttattcccgg aaattgacgc actgtttggc gttccggcac ctgccaggtg
     7381 gcatccggaa atcgacacgg gtattcatac cttaatgacg ctctcaatgg cggcgatgct
     7441 gagtccgcag gttgatgtcc gtttcgcaac tttatgccac gatctcggta aaggactgac
     7501 gccgccagaa ctttggccgc gtcatcatgg gcatggcccg gcgggtgtta agttagtgga
     7561 acaactatgc cagcgtctgc gcgtgccaaa tgaaattcgc gatttagcca gactggtggc
     7621 tgagtttcac gatctcatcc gcaccttccc aatgctgaac ccaaaaacca tcgtcaaatt
     7681 atttgattcc atcgatgcct ggcgtaaacc gcagcgtatc gagcaactgg cgctgaccag
     7741 cgaggctgac gtgcgcggca gaaccggttt tgaatcagcg gactacccgc aaggccgttg
     7801 gttgcgcgaa ggctgggaag tggcgcagtc agtgccgaca aaagcggtcg ttgaagcggg
     7861 atttaaaggc gtggagattc gcgaggagct gacccgacgg cggattgcgg cggtagccag
     7921 ctggaaggaa caacgttgcc caaagcctga ctgagtataa aactaaacgt tagcacgaac
     7981 cgtcccctcg ccccttcggg gagagggtta gggtgagggg aacagaccag caccggtgcg
     8041 aacaatcacc cccaccccgg ccctctccct acagagcgag ggagaaaacc cggcctgctc
     8101 ccgaaccttt tctcaatcaa aagaacacga catacaccgc agccgccaca ataaagcgat
     8161 aaatggcgaa cgggataaac gaaatgcgct taatcaattg caggaaggtt ttaatcgcta
     8221 tcagcgccac cacaaaagcg gtgataaacc caacggcaaa catcgggata tcgccggttg
     8281 tcaggaagcc ccagcttttg tagagatcga gcgccgttgc gcccatcatc atcggtaccg
     8341 ccagcaggaa cgagaactcg gaagcagcgt aacggctcac gcccatcagc atcccacctg
     8401 aaatggtcgc cccggaacgg gagaaacccg gccacagcgc cagacactgg aaacagccaa
     8461 tcataaacgc ctgacgatag gtcatatcat caagacccgg cgcacgcggc tctttcggct
     8521 tcaggcattc ggcggcaatc agcaacaaac cgccaacgac cagcgcatac atcacattta
     8581 tcgggttaaa caatgactta atcgtgtcgt ggaataatag ccccagtacc accgccggaa
     8641 tcatccccag caaaatgtgg atcagcgtta aacgaccttt gctttcacct tcgtgctgca
     8701 acgggcggcc aaagtggatg ccaatcaggc caaacagacg ccgccagaac atcactacta
     8761 ccgccagaat tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg tcgccctcaa
     8821 accccaacaa gtgaccgaca ataatcatat ggcccgtgct ggataccggc agaaattctg
     8881 tcaatccttc gaccacaccc aatattgccg ctatcagcag cgagtgcata tcgctcatca
     8941 ataaacccct aaattattaa aatgtaccgc ttgtccgaac tactgcagta tgaccaggtt
     9001 ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct
     9061 caatgattac gccaacattc gccgcccgcg ccactgcgcc tggcttgctg agtttgatac
     9121 gcacccacgg cgagttgaag cgtgctaaca gcagctccgc cacctcttca gccacgcgtt
     9181 ccaccagcgc aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt
     9241 aactgaggca atccgccaca tcatcacttt tcgccgcttt acggttatcc catgccattt
     9301 cgatatcgaa cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg
     9361 tgattaccga aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct
     9421 aaccggatac cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga
     9481 attttcaaaa cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac
     9541 ctctgcggct ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg
     9601 cgaaccagcg gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga
     9661 gcagccgtag cagtactgat tttcgacgtt ctgaaaggaa tgttgcctgt ctggggcgcg
     9721 tatgaattag gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga
     9781 cacatctggc ccgttttctt cggttttaaa ggaggaaaag gcgttgctac cgcttttgga
     9841 gcaatcgcac caattagctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc
     9901 gtgctattga gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt
     9961 tatgtctggt ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc
    10021 ctgctgcgtc atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg
    10081 acgaaattca aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac
    10141 ctgatagcct tcatcgggct tgcccagccg ttgctggcac catgccgcca gaaattccgc
    10201 gcagacacga atttcatgct gcgatagagc agctcccggt aaacagccca gatattggcg
    10261 ctttgtgcat actccggcaa tacttgcacc agtttgccac tctccagaaa cggcaacaca
    10321 tcccactcgg aacgcagcat aatccctttg ccctccagcg cccattgcaa cactatctcg
    10381 ccgctattgg aggaaagatg cccgctaacc ttcaccgatt ttttctcctg accgttcccc
    10441 aactcccata ttccgtgggt catatcgcgc tctttggtca ccagacagtc atgacgactt
    10501 aattcctgta atgattgtgg ctgcggatat ttttgcagat actcaggtgc tgcgcataat
    10561 attcttttat ttttcgttaa cagatgcgca atataataat caggaatttc gtcattaata
    10621 cgaatatcca gatcaatatt atcctgcact aaatcaattt gccgatcgaa caattcaaaa
    10681 tgcacctgta actcaggata attgcgcata agttcggtaa tggctggcgc aatatggctg
    10741 cgcccaaaac caaaactgca accaatacga atcatcccct ccgggcgcgt tttgatttgc
    10801 gtgacgtcat cgaccagtcg ctgatattgg gtgaggattt ccagcgcatg ttcgtaacaa
    10861 cgttggccac tttcagtcag cgccacgccg cgagcggagc ggttcagcag cgttgttgcc
    10921 agcgtatttt caaggatttg gatccgcttg gtgacaaatg caggcgtttg cccaagcgtc
    10981 gccgctgcag cactaaaact cccgctgtgg acgatttcta ccagtacctg caagtctttg
    11041 gctaaaggcc agctattcag catcgttaat tatccgcagt tgtgataagc gcagtgtatt
    11101 tcgcaaaaca ttgccattcc ctgctctggc gcaatattca ttcttatttt cgcgttgatt
    11161 cacgaactgt taattacata ttaacggtgg caaaaaatat aaaaccacat ttttagtgtt
    11221 agtttgtggc ggtgaatttt tccagacaaa tacaaaaact ggagttgcca tgatgagcga
    11281 aagtaataag caacaggcag tgaataagtt gacagagatt gtcgctaact ttaccgccat
    11341 gatttctacc cgaatgcctg atgacgtggt ggataaacta aaacagctaa aggatgccga
    11401 aacgtcgtcg atggggaaaa ttatctacca tacgatgttc gacaacatgc aaaaagcgat
    11461 tgacctgaat cgtcctgcct gtcaggacac cggggagatt atgttcttcg ttaaagtcgg
    11521 ttcccgcttc ccactgcttg gcgagctgca aagcatactc aaacaagccg tggaagaggc
    11581 aaccgtcaaa gcgccactac gtcacaatgc ggtagaaatt tttgacgaag taaacaccgg
    11641 caaaaatacc ggtagcggcg taccgtgggt cacctgggac atcatccccg acaatgacga
    11701 tgcggaaatc gaagtttaca tggcaggcgg cggctgcacg ctacctggcc gctcgaaagt
    11761 gttaatgccg tcagaaggct acgaaggcgt ggtgaaattc gtcttcgaaa atatctccac
    11821 cctcgccgta aacgcctgtc caccggtact ggtgggcgtg ggcatcgcca cctcggtgga
    11881 aaccgccgcc gtactctcgc gtaaagccat tttgcgcccg attggcagcc gccatcccaa
    11941 tccaaaagcg gcagaactgg agctacgcct ggaagaagga ctcaaccgtc tggggattgg
    12001 tccacaaggg ctgaccggca acagttcagt gatgggcgca tcgtcgcggc acgctgctgg
    12061 ttcatgccga tctcaccttt gaaaatctgt ctcacacccg gagcgcgtta tgaaaaagat
    12121 cctgacaacc ccgatcaaag ctgaagatct gcaagatatt cgcgtcggcg atgtgatcta
    12181 cctgaccggt acgctggtga cctgccgcga cgtttgtcac cgccgtttga tcgaactgaa
    12241 acgtccgatc ccttacgatc tcaacggcaa agcgattttc cacgctggcc ccatcgtgcg
    12301 caaaaacggc gacaaatggg agatggtctc cgtcggcccg acaaccagta tgcgtatgga
    12361 aagttttgaa cgtgaattta ttgagcagac cggcgtaaaa ctggtggttg gcaaaggtgg
    12421 tatggggccg ctgaccgaag aaggctgcca gaaattcaag gcgttacatg tgattttccc
    12481 agcaggctgc gcggtgctgg cggcaaccca ggtagaagag attgaagaag tgcactggac
    12541 agaactcggg atgccggagt cactgtgggt ctgccgggtc aaagagttcg gcccgctgat
    12601 tgtctctatt gatacccacg gcaacaacct gatagccgaa aacaaaaagc tgttcgccga
    12661 acgccgcgat cccatcgtgg aagagatctg cgagcacgtc cattacatca aataaccctc
    12721 ccggagaggc tcacccctct cctttttcgc aggcataaca cgatgaaacc ttccactgaa
    12781 tggtggcgat atcttgcgcc gctggcggtc atcgccatta ttgctctact tccggttccc
    12841 gcagggctgg agaaccatac ctggctctac tttgccgttt ttactggcgt gatcgttgga
    12901 ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg tgggtatctc catcatcgcc
    12961 atactctctc cctggctgct gttcagcccc gaacagctcg cccagccagg ctttaagttc
    13021 accgccaaat ccctctcctg ggcagtttct ggtttttcta attcggttat ctggctcatt
    13081 ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg ggcttggacg ccgcattgcg
    13141 ctgattctgg tgaaaaagat ggggcatcgc acgctgtttc tcggctatgc ggtgatgttc
    13201 tccgagctga tcctggcacc tgtaacaccg tccaactcgg cgcgtggtgc gggtattatc
    13261 tatcccatca tccgtaacct gccaccgctc tatcaatcac aaccaaacga cagcagttcg
    13321 cgcagcattg gctcgtacat catgtggatg gggattgttg ccgactgcgt gaccagcgcc
    13381 attttcctga cggcgatggc acctaacttg ctgttaattg gactgatgaa aagcgcatct
    13441 cacgccacgc tgagttgggg cgactggttc ctcgggatgt tgccgctcag cattttactg
    13501 gttctgctgg ttccctggct ggcttacgtg ctgtacccgc cggtactgaa gtctggtgat
    13561 caggtgccgc gctgggcaga gacggaactg caggcaatgg gcccgctctg ttcgcgtgaa
    13621 aaacggatgc tggggctgat ggtaggcgcg ctggtgctgt ggattttcgg cggtgattat
    13681 atcgatgccg cgatggtcgg ttacagcgtg gtggcgctga tgctgcttct gcgcattatc
    13741 agttgggacg acattgtcag taataaagcg gcatggaacg ttttcttctg gctggcctcg
    13801 cttatcaccc tcgcgaccgg actcaacaat accggtttta ttagctggtt tggcaaactg
    13861 ttagcaggca gcttaagtgg ttattcgccg acgatggtga tggtgacgtt gattgtggtg
    13921 ttttatctac tgcgctactt tttcgccagc gccacggcgt atacctgcgc cctcgcaccg
    13981 atgatgatcg ccgccgcgct ggcgatgccg gaaatcccgc tgccggtgtt ctgcctgatg
    14041 gttggcgcgg caattggtct ggggagcatt cttacaccat acgccaccgg acccagtccg
    14101 atttactacg gtagtggtta tctgccaacg gcggattact ggcgactggg ggcgattttt
    14161 gggctgatat tcctcgtgtt gctggtgatt accggcttac tgtggatgcc cgtggtgttg
    14221 ctttaagtga ggacggtgcc ggaaattgct ctccggcacc caatagtttt acgcagccgg
    14281 taactccgcc agcggccagc gcggacgcac gctaacgccg agatccgccg tcgcgcctgc
    14341 tttaaaccgc accattccgg cataggcgat catcgcgccg ttatcagtac aaaattccgg
    14401 acgcgcgtag aacacttcgc cgcggcgttt tttcatcatt tcagccagct tcgcccgtaa
    14461 cgtgcggtta gcactcacgc cgcccgccat gaccagtcgc ttaaagcccg tctgatccaa
    14521 cgctcgctta cacttaatca tcaacgtatc gaccaccgca tcttcaaagg cgcgggcgat
    14581 atcagcacgc gtctggtcgt cggtgccgtt gtcacgaatc gtgttcgccg caaaggtttt
    14641 cagaccagaa aagctgaaat ccagccccgg acggtcggtc atcggacgcg ggaaaacaaa
    14701 gcgcccggca gtaccctgag ccgccatttt cgacagtaac ggtccgccag gataatccag
    14761 ccccagcagc ttcgcggttt tatcaaacgc ttcaccggcg gcatcatcga tagactcgcc
    14821 gagcagctcg tactgaccaa tgccagtcac gctgattaac tgcgtatgac cgccggacac
    14881 cagcagcgcg acaaacggaa attccggcgg gttatcttcc agcatcggcg ctaacagatg
    14941 cccttccata tggtgtaccg ggattgccgg aacgttccag gcaaacgcca gagaacgccc
    15001 cacggttgcg ccaaccagca gtgcgccgac taatccaggg cctgcggtat aggccacagc
    15061 atcaatgtct tttgccgtta aaccagactc ctttagcgcc gcctggatca acggtacggt
    15121 tttacgcaca tgatcgcggg aggccagttc aggcacgacg ccgccgtagt cagcgtgcaa
    15181 tttcacctga ctatacaatt ggttggctaa caaacctttt tcatcgtcgt aaatggcgat
    15241 gccggtttca tcgcaggaag tttcaatacc cagtacacgc atgacttgtt ttacctcgct
    15301 ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa aactttgttc gcccctggag
    15361 aaagcctcgt gtatactcct cacccttata aaagtccctt tcaaaaaagg ccgcggtgct
    15421 ttacaaagca gcagcaattg cagtaaaatt ccgcaccatt ttgaaataag ctggcgttga
    15481 tgccagcggc aaaccgaatt aatcaaaggt gagaggcaca tgccggtaat taaagtacgt
    15541 gaaaacgagc cgttcgacgt agctctgcgt cgcttcaagc gttcctgcga aaaagcaggt
    15601 gttctggcgg aagttcgtcg tcgtgagttc tatgaaaaac cgactaccga acgtaagcgc
    15661 gctaaagctt ctgcagtgaa acgtcacgcg aagaaactgg ctcgcgaaaa cgcacgccgc
    15721 actcgtctgt actaattccc cgagagcgtt gctctccgat cagaccgagt tgtagttgta
    15781 aggccgtgct tccgaaagga atgcgcggct tattttcgtt tatgaattgc taaaaatcgg
    15841 ggcctatggc tggacgaatc ccacgcgtat tcattaatga tctgctggca cgcactgaca
    15901 tcgtcgatct gatcgatgcc cgtgtgaagc tgaaaaagca gggcaagaat ttccacgcgt
    15961 gttgtccatt ccacaacgag aaaaccccgt cattcaccgt taacggtgag aaacagtttt
    16021 accactgctt tggatgtggc gcgcacggca acgcgatcga cttcctgatg aactacgaca
    16081 agctcgagtt cgtcgaaacg gtcgaagagc tggcagcaat gcacaatctt gaagtgccat
    16141 ttgaagcagg cagcggcccc agccagatcg agcgccatca gaggcaaacg ctttatcagt
    16201 tgatggacgg tctgaatacg ttttaccaac aatctttaca acaacctgtt gccacgtctg
    16261 cgcgccagta tctggaaaaa cgcggattaa gccacgaggt tatcgctcgc tttgcgattg
    16321 gttttgcgcc ccccggctgg gacaacgtcc tgaagcggtt tggcggcaat ccagaaaatc
    16381 gccagtcatt gattgatgcg gggatgttgg tcactaacga tcagggacgc agttacgatc
    16441 gtttccgcga acgggtgatg ttccccattc gcgataaacg cggtcgggtg attggttttg
    16501 gcgggcgcgt gctgggcaac gataccccca aatacctgaa ctcgccggaa acagacattt
    16561 tccataaagg ccgccagctt tacggtcttt atgaagcgca gcaggataac gctgaaccca
    16621 atcgtctgct tgtggtcgaa ggctatatgg acgtggtggc gctggcgcaa tacggcatta
    16681 attacgccgt tgcgtcgtta ggtacgtcaa ccaccgccga tcacatacaa ctgttgttcc
    16741 gcgcgaccaa caatgtcatt tgctgttatg acggcgaccg tgcaggccgc gatgccgcat
    16801 ggcgagcgct ggaaacggcg ctgccttaca tgacagacgg ccgtcagcta cgctttatgt
    16861 ttttgccgga tggcgaagac cctgacacgc tggtacgaaa agaaggtaaa gaagcgtttg
    16921 aagcgcggat ggagcaggcg atgccactct ccgcatttct gtttaacagt ctgatgccgc
    16981 aagttgatct gagtacccct gacgggcgcg cacgtttgag tacgctggca ctgccattga
    17041 tatcgcaagt gccgggcgaa acgctgcgaa tatatcttcg tcaggaatta ggcaacaaat
    17101 taggcatact tgatgacagc cagcttgaac gattaatgcc aaaagcggca gagagcggcg
    17161 tttctcgccc tgttccgcag ctaaaacgca cgaccatgcg tatacttata gggttgctgg
    17221 tgcaaaatcc agaattagcg acgttggtcc cgccgcttga gaatctggat gaaaataagc
    17281 tccctggact tggcttattc agagaactgg tcaacacttg tctctcccag ccaggtctga
    17341 ccaccgggca acttttagag cactatcgtg gtacaaataa tgctgccacc cttgaaaaac
    17401 tgtcgatgtg ggacgatata gcagataaga atattgctga gcaaaccttc accgactcac
    17461 tcaaccatat gtttgattcg ctgcttgaac tgcgccagga agagttaatc gctcgtgagc
    17521 gcacgcatgg tttaagcaac gaagaacgcc tggagctctg gacattaaac caggagctgg
    17581 cgaaaaagtg atttaacggc ttaagtgccg aagagcgatc gggaagcccc cgacagccgc
    17641 actgagaggc agcggcaaat atataagtac gccctcgtaa ttatcgttgg cggtaaacaa
    17701 ccgttggatt tcagcgttaa cggctgaagg acatcgggtc aatcgcccaa caccaacctc
    17761 atgaaataag tgtggatacc gtcttatgga gcaaaacccg cagtcacagc tgaaacttct
    17821 tgtcacccgt ggtaaggagc aaggctatct gacctatgcc gaggtcaatg accatctgcc
    17881 ggaagatatc gtcgattcag atcagatcga agacatcatc caaatgatca acgacatggg
    17941 cattcaggtg atggaagaag caccggatgc cgatgatctg atgctggctg aaaacaccgc
    18001 ggacgaagat gctgccgaag ccgccgcgca ggtgctttcc agcgtggaat ctgaaatcgg
    18061 gcgcacgact gacccggtac gcatgtacat gcgtgaaatg ggcaccgttg aactgttgac
    18121 ccgcgaaggc gaaattgaca tcgctaagcg tattgaagac gggatcaacc aggttcaatg
    18181 ctccgttgct gaatatccgg aagcgatcac ctatctgctg gaacagtacg atcgtgttga
    18241 agcagaagaa gcgcgtctgt ccgatctgat caccggcttt gttgacccga acgcagaaga
    18301 agatctggca cctaccgcca ctcacgtcgg ttctgagctt tcccaggaag atctggacga
    18361 tgacgaagat gaagacgaag aagatggcga tgacgacagc gccgatgatg acaacagcat
    18421 cgacccggaa ctggctcgcg aaaaatttgc ggaactacgc gctcagtacg ttgtaacgcg
    18481 tgacaccatc aaagcgaaag gtcgcagtca cgctgccgct caggaagaga tcctgaaact
    18541 gtctgaagta ttcaaacagt tccgcctggt gccgaagcag tttgactacc tggtcaacag
    18601 catgcgcgtc atgatggacc gcgttcgtac gcaagaacgt ctgatcatga agctctgcgt
    18661 tgagcagtgc aaaatgccga agaaaaactt cattaccctg tttaccggca acgaaaccag
    18721 cgatacctgg ttcaacgcgg caattgcgat gaacaagccg tggtcggaaa aactgcacga
    18781 tgtctctgaa gaagtgcacc gcgccctgca aaaactgcag cagattgaag aagaaaccgg
    18841 cctgaccatc gagcaggtta aagatatcaa ccgtcgtatg tccatcggtg aagcgaaagc
    18901 ccgccgtgcg aagaaagaga tggttgaagc gaacttacgt ctggttattt ctatcgccaa
    18961 gaaatacacc aaccgtggct tgcagttcct tgacctgatt caggaaggta acatcggcct
    19021 gatgaaagcg gttgataaat tcgaataccg ccgtggttac aagttctcca cctacgcaac
    19081 ctggtggatc cgtcaggcga tcacccgttc tatcgcggat caggcgcgca ccatccgtat
    19141 tccggtgcat atgattgaga ctatcaacaa actcaaccgt atttctcgcc agatgctgca
    19201 agagatgggc cgtgaaccga cgccggaaga actggctgaa cgtatgctga tgccggaaga
    19261 caagatccgc aaagtgctga agatcgccaa agagccaatc tccatggaaa cgccgatcgg
    19321 tgatgatgaa gattcgcatc t
//
LOCUS       DAJTYM010000079        18205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTYM010000079 DAJTYM010000000
VERSION     DAJTYM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="OO575_002815"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="OO575_002815"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6665713.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="OO575_002816"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="OO575_002816"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6665714.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO575_002817"
     CDS             2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO575_002817"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6665715.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="OO575_002818"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="OO575_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6665716.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="OO575_002819"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="OO575_002819"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6665717.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="OO575_002820"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="OO575_002820"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6665718.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="OO575_002821"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="OO575_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6665719.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="OO575_002822"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="OO575_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6665720.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="OO575_002823"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="OO575_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6665721.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="OO575_002824"
     CDS             11296..11511
                     /locus_tag="OO575_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6665722.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO575_002825"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO575_002825"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6665723.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="OO575_002826"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="OO575_002826"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6665724.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="OO575_002827"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="OO575_002827"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6665725.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="OO575_002828"
     CDS             complement(16247..16786)
                     /locus_tag="OO575_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6665726.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="OO575_002829"
     CDS             complement(16802..17320)
                     /locus_tag="OO575_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6665727.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="OO575_002830"
     CDS             complement(17631..17822)
                     /locus_tag="OO575_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6665728.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="OO575_002831"
     CDS             17840..17992
                     /locus_tag="OO575_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665729.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT         4310 a         4449 c         5000 g         4446 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa
    18001 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
    18061 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
    18121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
    18181 tgaatgcaac ttatataaaa atacg
//
LOCUS       DAJTYM010000080        17689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTYM010000080 DAJTYM010000000
VERSION     DAJTYM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..401
                     /locus_tag="OO575_002832"
     CDS             <1..401
                     /locus_tag="OO575_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6665730.1"
                     /translation="FEAGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISC
                     PKEVAGVKLSLITNDKITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAI
                     PFKAKYLKLTDNSVQSGDVLSSLVMRVAQD"
     gene            482..1156
                     /locus_tag="OO575_002833"
     CDS             482..1156
                     /locus_tag="OO575_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6665731.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1172..3634
                     /locus_tag="OO575_002834"
     CDS             1172..3634
                     /locus_tag="OO575_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6665732.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3650..4683
                     /locus_tag="OO575_002835"
                     /pseudo
     CDS             3650..4683
                     /locus_tag="OO575_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4765..5103)
                     /gene="rcnB"
                     /locus_tag="OO575_002836"
     CDS             complement(4765..5103)
                     /gene="rcnB"
                     /locus_tag="OO575_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6665733.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5322..6152)
                     /gene="rcnA"
                     /locus_tag="OO575_002837"
                     /pseudo
     CDS             complement(5322..6152)
                     /gene="rcnA"
                     /locus_tag="OO575_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6274..6546
                     /gene="rcnR"
                     /locus_tag="OO575_002838"
     CDS             6274..6546
                     /gene="rcnR"
                     /locus_tag="OO575_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6665734.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6638..6734
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6769..7557
                     /gene="thiM"
                     /locus_tag="OO575_002839"
     CDS             6769..7557
                     /gene="thiM"
                     /locus_tag="OO575_002839"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6665735.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7554..8354
                     /gene="thiD"
                     /locus_tag="OO575_002840"
     CDS             7554..8354
                     /gene="thiD"
                     /locus_tag="OO575_002840"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6665736.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8419..9240
                     /locus_tag="OO575_002841"
     CDS             8419..9240
                     /locus_tag="OO575_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6665737.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9288..10034
                     /locus_tag="OO575_002842"
     CDS             9288..10034
                     /locus_tag="OO575_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6665738.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10008..10973)
                     /locus_tag="OO575_002843"
     CDS             complement(10008..10973)
                     /locus_tag="OO575_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6665739.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(10970..11973)
                     /locus_tag="OO575_002844"
                     /pseudo
     CDS             complement(10970..11973)
                     /locus_tag="OO575_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12013..13065
                     /gene="fbaB"
                     /locus_tag="OO575_002845"
     CDS             12013..13065
                     /gene="fbaB"
                     /locus_tag="OO575_002845"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6665740.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13373..14227
                     /gene="gatY"
                     /locus_tag="OO575_002846"
     CDS             13373..14227
                     /gene="gatY"
                     /locus_tag="OO575_002846"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6665741.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14256..15518
                     /gene="gatZ"
                     /locus_tag="OO575_002847"
     CDS             14256..15518
                     /gene="gatZ"
                     /locus_tag="OO575_002847"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6665742.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15528..15980
                     /gene="gatA"
                     /locus_tag="OO575_002848"
     CDS             15528..15980
                     /gene="gatA"
                     /locus_tag="OO575_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6665743.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16011..16295
                     /gene="gatB"
                     /locus_tag="OO575_002849"
     CDS             16011..16295
                     /gene="gatB"
                     /locus_tag="OO575_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6665744.1"
                     /translation="MKRKIIVACGGAVATSAMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16299..17654
                     /locus_tag="OO575_002850"
     CDS             16299..17654
                     /locus_tag="OO575_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6665745.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4599 a         4061 c         4518 g         4511 t
ORIGIN      
        1 aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca accactgtct
       61 ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc agcagcatgt
      121 taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca
      181 aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtaggt gataacagcg
      241 atgtcctaga tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat
      301 atgctattcc gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg
      361 atgtgttatc ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt
      421 ctccaggtaa taaacatggc cgctatcccc atggcggctt tttaatttaa gaggcattaa
      481 gatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat
      541 tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt
      601 gatgaaccag ggaaatcgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc
      661 gttaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg
      721 ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa
      781 agaaagcatt ttttatctga atgttctgta tattccacca aatagtccag agcaagaagg
      841 caagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg
      901 tattgctccg gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg
      961 attggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa
     1021 tgttaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatat
     1081 caaaagtaat aatgcaaata actggtatct gaccattatc aatgaccacg gcaactatat
     1141 tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc
     1201 aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca
     1261 ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca
     1321 acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata
     1381 tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg
     1441 gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact
     1501 tgttcagggt gggagctata gctgggatat cggggttttt cgtctcgatt tcagtgtccc
     1561 gcaggcttgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg
     1621 tattaatgcg ttttatactt cttattatgt gagtcagtat tacagcgact ataaagcgtc
     1681 gggtaacaac aagagtacat atgtacgttt taacagcggg ttaaatttac tggagtggca
     1741 actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa
     1801 taccctgtat ctggaacgtg gatttgccca atttctcggc acgcttcgcg tgggtgatat
     1861 gtacacatca agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga
     1921 tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca
     1981 gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc
     2041 tcctggcccg tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt
     2101 tagcgtgaaa gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcggtgcc
     2161 aaatatgctg caacccggcg tgtcgaaata tgattttgcg gcgggggcga gcaagcaaag
     2221 tgattttgtc caggcaggtt atcagtatgg ttttaataac ttattgacgc tgtatggcgg
     2281 cacgatggtt gctaataatt actatgcgtt taccctcgga acgggttgga acacgcgcat
     2341 tggcgcaatt tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt
     2401 tgacgggcaa agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt
     2461 tggtctggcg gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg
     2521 ggcaaacaat aaagataatt atcgccgtga tgaaaacgat atctatgaca ttgccgatta
     2581 ttaccagaac gattttggcc gtaaaaatag cttttctgcc aatatgagcc agtcattgcc
     2641 agaaggctgg ggttctgtgt ccttaagtac gttatggcga gattactggg ggcgtagcgg
     2701 cagcagtaag gattatcagt tgagttattc caataactgg cggcggataa gctataccct
     2761 cgcggcaagc caggcgtatg gcgagaatca tcatgaagag aaacgtttta atatttttat
     2821 atcaattccc tgtgattggg gtgatgacgt tacgacgcct cgtcggcaaa tatatatgtc
     2881 taactcaacg acgtttgatg atcagggttt tgcctcaaat aatacgggat tatcaggaac
     2941 agtagggagt cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa
     3001 tgaaacgaca gctggggcga atttgacctg gaacgcgccg gttgcgacag tgaatggcag
     3061 ttatagtcag tcgagtactt atcgacagac tggagccagt gtttcagggg gcattgtcgc
     3121 ctggtcgggt ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc
     3181 gccaggaatt aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg
     3241 agtggtggta tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc
     3301 gcaaagcgat agcgaagcag aattacgtgg caaccggaaa attgccgccc cttatcgcgg
     3361 cgcggttgta ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt
     3421 aagagcggat ggtcaaccat taacgtttgg ttatgaagtc aatgatatcc atggtcataa
     3481 tattggcgtt gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc
     3541 ggttaatgta gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga
     3601 gattgatgaa agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg
     3661 cataatgcta tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag
     3721 tgagttatct gttcagcaca atttggttgt tcaggggaat tttgcactta ctcaaacaca
     3781 aatagcgaca tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac
     3841 ccctatgagc ccatcggata ttattgttgg actttataac gataccatta aattaaattt
     3901 acattttgaa tggaccaata aaaacaacat cacgttgtca aataatcaga gcagtttcac
     3961 cagtggttat tcggtaacgg tgacacctgc ggccagtaat gcaaaagtga atatttctgc
     4021 ggggggcggt ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc
     4081 gacacgcggg agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatgggg
     4141 tgcatgtgta aatagctaca gaaatgcatt ggcacaaaat gcaggtgtct attccttcaa
     4201 tctgacattg tcatacaacc cgataaccac aacctgcaaa caggacgatt tattaattac
     4261 tttagacagt attcccgttt tacaattacc agccacaggt aacaaagcaa caataaatag
     4321 taaaaaaagg gatattattc tgcgttgtaa aaatttatta ggtcaacaaa atcaaacatc
     4381 acggaaaatg caggtgtatt tatcaagttc tgacttgtta accaacagca acacaatact
     4441 gaaaggtgcg gaagataatg cgtaggattt attcttgaaa gtaatggttc gccagtcaca
     4501 cttttaaata tcactaacag cagtaaagta tacacaaatt taaaagaaat tgcggcaaag
     4561 tcaaaactta cagatacaac ggtttcaatt ccgataacag ccagttacta cgtctacgat
     4621 acaaacaaag ttaaatctgg cgcactggag gcaaccgcat taatcaacgt gaaatacgac
     4681 taataacaag caaaacgagc aagtggctaa taataaaaaa taacaaggtg tctgcatttc
     4741 cccctcatga tgagggggct ttttttagcg atgataaaaa atctcaccgt cgtaggcttt
     4801 aatgatttta ccgtcggtgt cgctgatcaa cacgtacgcg ccacccatat aggtccagtg
     4861 cgtcccggca tcaggcgcgg gcaggttacg taactgccac tgcttaatgt tgtactcatc
     4921 ggtgcggtac atctctggga cggtatcacc tggtttgaaa tgcttaaagt cagcaatgaa
     4981 actacttaac tcatacttag aaattcccga ggtattggtc gaacccgcgg tggctggtgc
     5041 ggcccaggcg gcactggtaa ccagcaaaag cacacccagc aacatcttat ttttaatggt
     5101 cataaactct ccactttggg gatctgtaat atcagtcgca tatctttcct gtcatacaat
     5161 ataactgaca agcgatattt tgttagtgat cccatgattt cgtaaaaagt gtaaatgaaa
     5221 ggatgcccta aaagcatcct tagcgagcag agagtcagcg gaaatagctg atttaagaat
     5281 catcctgggc tgaagataac atgcaagcct aaaggattga gttatcgcat tatgcccatg
     5341 aagccatgta cgcccatata cacaccgact aagccaatca acagactgga aaaatagggg
     5401 gcgcgtttag cgagagtgtt aaatccgctc cagcgttttg cgacctgctg aacgctgatt
     5461 gctgcgccaa cgcctacggt gacaagcgtt aacgccaggc caatgctgaa actgacgacc
     5521 agtgttgcgc ccagtgtcag ggctttcaac tgaatgcata tcaacagcac ggtaattgct
     5581 gccgggcagg ggataaagcc accggttaag ccaaataaca aaatttgcca gttgatgacc
     5641 tctctaccat caaagcgtcg tttaatgtca ttggcatggg ctcgtgcatg ggcatcctga
     5701 tactcgccat gttcgtgatg gtgatgatgt ccttggtcgt ggtgatgttc gtgatcgtga
     5761 tgatggtgat gatgctcata atcatgcccg tgcatattct ccagccagtt gcgttcgccg
     5821 cgctaggtac gccagaacat ccagaacgcg gtgccaataa tgatcactgc ggaaatcagc
     5881 tggagccacg gttctgctga ttgagcagta aagcgcttac tgatcaccat cccgccaaag
     5941 gcaatcaacc agaccaccgc ggtatgcgaa atggttgctg ccagtccgag catcaccgcc
     6001 tgtttaatgg tgcctttgat ggcgatgata aacgccgcca tcatcgtttt tgagtgccct
     6061 ggttccaggc catgaagcgc accaagtaag atggcgctgg ggatgaagaa ccaggcgttt
     6121 ccttgctgaa gaagagttgt aaattcggtc atgataataa ttcttagtat taattcggca
     6181 atctgattct actccccccc agtacctgat actacccccc cagtagatta atagtgctat
     6241 gatttttcat attcttgtta accaggtgtt gccatgtctc atacaatccg tgataaacag
     6301 aaactgaaag cgcgtgccag taagattcag ggccaggtcg tggcgctcaa gaaaatgctc
     6361 gacgagccgc acgaatgcgc tgcagtttta caacagattg ctgctatccg tggagcggta
     6421 aacggtctga tgcgggaagt gattaaaggt catctgacgg aacacatcgt tcaccagggg
     6481 gatgagctaa aacgtgaaga agatctggat gtcgttctga aggtgctgga ttcatatatc
     6541 aaataattta ttaacgcgat tgtaaaactg ccgtttttcc tcgtttacaa cgggggcgct
     6601 ggacattacc atcctcctct gcgatttatc atcgcaacca aacgactcgg ggtgcccttc
     6661 tgcgtgaagg ctgagaaata cccgtatcac ctgatctgga taatgccagc gtagggaagt
     6721 cacggaccac caggtcattg cttcttcacg ttatggcagg agcaaactat gcaagtcgac
     6781 ctgctgagtt cagcgcaatc tgcgcacgcg ttacaccttt ttcaccaaca ttcccctctt
     6841 gtgcactgca tgaccaatga tgtggtgcaa acctttaccg ccaatacctt gctggcgctc
     6901 ggtgcatcgc cagcgatggt tatcgaaacc gaagaggcca gtcagtttgc ggctatcgcc
     6961 agtgccttgt tgattaacgt tggcacactg acgcagccac gcgcacaggc gatgcgtgct
     7021 gccgttgagc aagcaaaaag ctctcaaaca cgctggacgc ttgatccagt agcggttggt
     7081 gcgctcgatt atcgccgcca tttttgtcat gaacttttat cttttaaacc ggcagcgata
     7141 cgtggtaatg cttcggaaat catggcatta gctggcattg ctaatggcgg acggggagtg
     7201 gataccactg acgccgcagc taacgcgata cccgctgcac aaacactggc acgggaaact
     7261 ggcgcaatcg tcgtggtcac tggcgaggtg gattatgtta ccgatggaca tcgtatcatt
     7321 ggtattcacg gtggcgatcc gttaatgacc aaagtggtag gaactggctg tgcattatcg
     7381 gcggttgtcg ctgcctgctg tgcgttacca ggcgatacgc tggaaaatgt cgcatctgcc
     7441 tgtcactgga tgaaacaagc cggagaacgc gcagtcgcca gaagcgaggg gccaggcagt
     7501 tttgttccac atttccttga tgcgctctgg caattgacgc aggaggtgca ggcatgaaac
     7561 gaattaacgc tctgacgatt gccggtactg atccgagtgg tggtgcgggg attcaggccg
     7621 atcttaaaac cttctcggca cttggcgctt atggttgctc agttattact gcactggtgg
     7681 cgcaaaatac ccgtggcgta cagtcggtgt atcgcattga gcctgatttt gtcgccgccc
     7741 agctcgattc ggtgttcagc gatgtgcgaa tcgataccac taaaatcggt atgttggcgg
     7801 aaaccgatat tgttgaagcg gtggcagaac ggttgcaacg ttatcagatc caaaacgtgg
     7861 tactcgacac cgttatgctg gcaaaaagcg gcgacccgct gctttcacct tcggcggttg
     7921 ctacgctgcg cagtcgatta ttgccacagg tttcattaat aacgccaaac ttgcccgaag
     7981 ctgccgcctt gctcgacgcg ccacacgcgc gcaccgaaca ggaaatgctg gaacaagggc
     8041 gatcgctgtt ggcgatgggc tgtggcgcag tgctaatgaa aggtggtcat ctggatgatg
     8101 agcaaagccc ggactggctg tttacccgcg agggtgaaca acggtttacc gcaccgcgca
     8161 ttatgaccaa aaacacccac ggcactggtt gtacactctc tgcggcgttg gctgcactac
     8221 gcccgcgcca tacaaactgg gctgacaccg tacaggaggc aaaaatctgg ctttcatcgg
     8281 cgttagccca ggccgacacg ctggaagttg gtcacggtat tggtccggtt caccacttcc
     8341 acgcctggtg gtgagcaccg tactatagct gctttggtta ttgtcagcct ttggcactga
     8401 cattcaggga tgaaaccaat gcagctaaga attaccagtc gaaaaaaact caccgctcta
     8461 ttatgcgctc ttgggctaat ttccatcgtt gcaatttatc cgcgtcagac ggtgaatttt
     8521 ttctattcga cagcagttca gattacagac tacatccact tctacggtta tcgcccggtt
     8581 aaatctttcg ctattcgcat tcctgccagt tacaccattc acggattaga tgtttcgcgc
     8641 tggcaggagc gtatcgactg gcagcgggtg gcaaaaatgc gcgacaacga tatccgctta
     8701 cagtttgctt ttattaaggc tacggaaggc gaaaagctgg tggaccccta tttttcgcgt
     8761 aactggcaat taagccgcga aaatggcctg ctgcgcgggg cgtatcatta tttctccccg
     8821 tcggtatccg cttcagttca ggcgcgatta tttctgcaaa cggtggattt ctcacaaggc
     8881 gatttccctg ccgtgctgga cgtagaagaa cggggaaaat tatcggcaaa agaattacgc
     8941 aagcgggtaa gtcagtggct aaaaatggtc gaaaaacgca cgggaaaaaa gccgattatt
     9001 tactcaggag ccgtttttta tcacaccaat ctggcgggct atttcaatga atatccgtgg
     9061 tgggtggctc actattatca acgtcgtccg gacaatgacg gcatggcctg gcgcttctgg
     9121 cagcattccg accgtggaca ggtagatggc atcaatggtc cggtggattt taatgtgttt
     9181 aatggcacgg ggatgagttg caggcattcg ttgatgggat taaagaaacg ccttaaataa
     9241 gaagcagtga tatactccca ccagactccc tggcaggaaa aagacaaatg gaacaagcgc
     9301 atacccagct gattgcccaa ctgaacgagc gtattttagc ggcggataac acgccgcttt
     9361 atattaagtt tgccgaaacg gtaaaaaatg ccgtgcgcag cggggtgctg gagcatggca
     9421 atattttgcc cggtgagcgt gatttaagtc agttaaccgg cgtttcgcgc attacggtgc
     9481 gcaaggcgat gcaggcgctg gaagaagagg gcgtggtgac gcgttcgcgc ggttacggca
     9541 cgcagatcaa caacatcttc gaatactcgt tgaaagaagc gagaggtttt tctcagcagg
     9601 tggtattgcg cgggaaaaag cccgatacgc tatgggttaa taagcgtgtc gtgaaatgtc
     9661 ccgaagaagt tgcgcagcag ctggcggccg aagcgggaag tgatgtcttt ttgcttaagc
     9721 gtattcgcta tgtcgatgaa gaagcggtat cgattgagga atcgtgggtt ccggcgcatt
     9781 taattcatga tgttgatgcc atcggaattt ctctttatga ctatttccgc agccagcaca
     9841 tttacccaca acgtacgcgt tcccgcgtta gcgcccggat gccggatgcc gagtttcagt
     9901 cacatattca gttagatagc aaaataccgg tgctggtgat caagcaagtg gcgcttgacc
     9961 aacagcaacg gcctattgag tacagcatta gccactgtcg cagcgatcta tacgtttttg
    10021 tgtgcgagga gtagttcctc gcgcgttggc gcacaatcac cgccccgatg cccgacaacc
    10081 cacgacgcca ctgcattgcc cagtaatacg gcatccgcca gtggcagacc agaggccagc
    10141 ccggcaagta cgccacctgc atgactgtcg cccgccccaa tggtgtctac aacttgcgtt
    10201 ggaaatgccg gaatgcagcc agaagcgtcg ttgctgaaat accatgcgcc ttctttatcg
    10261 agacgaacga tcaacggcgc ggcaaatttc tcctgccatt gcttgccaag tgttgttatc
    10321 tctgcggata aagcaaaacg ttcggcggca atctcagcct cttgacgatt gagtgacact
    10381 aaaggtcgac aggccatgat ccgcgccagt aatgcgtccg ggatatcgcc aatacgtggg
    10441 caaaaatcga taaacggcgt cacgtcttgc agcttttcca gccattccac taacaattcg
    10501 ccgcagggcg aggccagttg ataaccggaa aaatagagca ggttgccagg cgcaacggtt
    10561 aatcgtgcca accattggcg attccattga ttttccacac cgctgaatga cataaaagtg
    10621 cgttcgccat ccggctcaac cagtgccaga caccagccgt tatcgctttc ggcgttatcg
    10681 atcaggctga ttaacccctc tttcgccatc cggttgcgaa taatctccgc ccacacgccc
    10741 tgaccgagcg gcaaggcatt acccgcttcg atgccgaggc gctttaacgc cacggcaata
    10801 ttcagtgcgc agccgccaac gttaacgctc tgctgtttca gttcgatatc gcacccacgc
    10861 caggggaggg cataagcgtc ggcgatcacg tcaatgaccg ccgcgccgac gaccatcacc
    10921 ggctgacgcg tggttaattc aggcagcagt gtgtgtaatc gagcgccgct cataccgcct
    10981 cccgttgttg acgatatttc gccagcgctg tggcatagcg gttgaaatca agctgattta
    11041 ccgcatccag ctccgccttt aacgcaggat cgacagcgtt aatgccatgc aacgcgccgc
    11101 aaatcgccgt cgccatagca cctatggtgt ctgtgtcgcc gccaagattg gcgcacagga
    11161 cggcgcagcg gtttgggtcg gtttgtgcca gttcaactag cgcaatggcg cacggaacgg
    11221 actcaatagt gctggtgcct gcgccaacga cctggtaaag ctgttcgctg gcggattcgg
    11281 tgccgtcggc attacgcaca attttcagcg caatctccag acgtgccgcc agtgaggcgc
    11341 tgaacgtggt ggtgcgtttt tgctgtgcat gtcgcgcaat tgaaggcaat gaatcaacaa
    11401 tcgctgacca gcgttctccg tcaatggcac gagaaatcgc ccatgcgatg actaccgcgc
    11461 ctgcaaccgc cagatcggat ttatgggtcg ggctggacgc cagcgccaca tcatcaataa
    11521 aggaatcaac atcacgcgct ggaagcaaac aacctaatgg cgagacgcgc atcgctgcgc
    11581 cgttggttac gccgttgttt tccagttcag caacgggttt accgtcgcga atggcgttaa
    11641 gcgcaatctt cgaggtcgga cctagtacgt ttttgttaaa ggcgtcgaaa cgcaacgccc
    11701 agtcgagaat attacgccca atcagattcg gatcgatctt gccttcacat tccagtaacg
    11761 catccgccag acacaggcca tcgaggtatc gtcggtgaat tcggcgcggt taaaataaca
    11821 ggccgcgtta ttctcctttg gtccaggcag aaaacggtca atccagccaa agtgtgcttt
    11881 aacgcggctg cgtggccaaa gctcggaggg catccccatc gcatccccta acgcctgccc
    11941 ataaagagca ccgagaatac gttctgtttt catttaactt ccccttgaat cggaatttac
    12001 gggagcatag taatgacaga tattgcgcag ttgcttggca aagacgccga caacctttta
    12061 cagcaccgtt gtatgactat tccttctgac cagctttatc tccccggaca tgactacgta
    12121 gaccgcgtga tgattgacaa taatcgcccg ccagcggtgt tacgtaatat gcagacgttg
    12181 tacaacaccg ggcgtctggc tggcacagga tatctttcta ttctgccggt tgaccagggc
    12241 gttgagcact ctgccggagc ttcatttgct gctaacccgc tctactttga cccgaaaaac
    12301 attgttgaac tggcgatcga agcgggctgt aactgtgtgg catcaactta cggcgtgttg
    12361 gtgtcggtat cgcggcgcta tgcgcatcgc attccatttc tcgtcaaact taatcacaac
    12421 gagacgctaa gttacccgaa tacctacgat caaacgctgt atgccagcgt ggagcaggcc
    12481 ttcaacatgg gcgcggtggc ggttggtgcg actatctatt ttggctcgga agagtcacgt
    12541 cgccagattg aagaaatttc tgcggccttt gaacgcgcgc acgagctggg catggtgaca
    12601 gtgctgtggg cctatttgcg aaactccgcc tttaagaaag atggcgttga ttaccatgtt
    12661 tccgccgacc tgactggtca ggcgaaccat ctggcggcaa ccataggtgc agatatcgtc
    12721 aaacaaaaaa tggcggaaaa taacggcggc tataaagcga ttaattacgg ttataccgac
    12781 gatcgcgtgt acagcaagtt aaccagcgaa aacccgattg atctggtgcg ttatcagtta
    12841 gctaactgct atatgggccg ggctgggttg ataaactccg gcggtgctgc aggcggtgaa
    12901 actgacctca gcgatgcagt gcgtactgcg gttatcaaca aacgcgcagg cggaatgggg
    12961 ctgattcttg gacgtaaggc gttcaagaaa tcgatggctg acggcgtgaa actgattaat
    13021 gccgtgcagg acgtttatct cgatagcaaa attactattg cctgatacgc ctcatacgaa
    13081 cagcaccacc ccgtaggacg gataagacgt ttacactgca tccggcaacc tatgcctgat
    13141 gcgacgcgga agcgtcttat catgcctaca tagcactgcc acgtatgttt acaccgcatc
    13201 cggcataaaa acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct
    13261 ttcactcctc gaacaatttt catattgttg tttttgtgat cattatctcg acatttaaaa
    13321 gcaaataatt tcattatatt ttgaaatcga aagcaaacga caagatatga aaatgtacgt
    13381 ggtatcgaca aagcagatgc tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt
    13441 caatattcac aatctcgaaa cgatgcaagt ggtggtggaa accgctgcca acctgcatgc
    13501 gccggtcatc atcgccggaa cgcctggcac atttactcat gctggtacag aaaatctgtt
    13561 ggcgctggtc agcgcgatgg cgaagcaata tcaccatcca ctggcaattc atctcgacca
    13621 tcacacgaaa tttgacgata tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat
    13681 tgacgcctcg catttgcctt ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt
    13741 ttgccatcgc tttgatgtca gcgtcgaggc ggagctgggg caacttggcg gccaggaaga
    13801 tgatgtgcaa gtcaatgaag tcgatgcgtt gtacaccaac cccgcacagg cgcgtgaatt
    13861 tgccgaggca accgggattg attccctggc ggtcgccatc ggcacggctc acggtatgta
    13921 tgccagcgca ccggtgcttg atttttctcg actggagaac attcgccagt gggtgaactt
    13981 accgctggtg ctgcatggcg cgtcaggatt atcgactaag gatattcagc aaaccatcaa
    14041 actggggata tgcaaaatca acgttgcaac ggagctgaaa aatgccttct tgcagtcgtt
    14101 aaaaaattac ctgaccgagc accccgaagc gaccgatccc cgggattatt tgcagtcggc
    14161 taaatccgca atgcgcgatg tggtgagcaa agtgattgcc gattgtggct gcgagggcag
    14221 ggcataacgc acctgccatt taacaaggaa aaaacatgaa aacgttaatt gcccggcata
    14281 aagctggtga acatatcggc atatgttcag tctgttctgc ccatccgttg gttatcgaag
    14341 cggcgctggc atttgatcgc aacagcacgc gcaaagtgct gattgaagca acgtcaaacc
    14401 aggtcaatca atttggcggt tataccggaa tgacaccggc agactttcgc gaatttgttt
    14461 ttacgattgc cgataaagtt gggtttgcac gcgaacgcat tattctcggc ggcgatcatc
    14521 tgggaccaaa ctgctggcag caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc
    14581 tggtaaaagc atatgttcgt gctggcttca gtaaaattca tcttgatgcg tcaatgtcct
    14641 gcgcggggga taccataccg ttagcaccag aaacggttgc ggaacgagct gctgtgcttt
    14701 gctttgctgc ggaaagtgtg gcgacagatt gccagcgtga gcaactgagc tatgtcattg
    14761 gcactgaagt tccggttccg ggcggtgagg ccagcgccat tcagtcagta cacatcaccc
    14821 atgttgaaga tgccgccaat actttacgta cgcatcaaaa ggcctttatt gcccgtgggc
    14881 tgacagaggc gttaacacgt gtgattgcca tcgtggtgca gccgggtgtg gaatttgatc
    14941 acagcaatat tatccattat cagccgcagg aagcgcaggc gctggcgcaa tggatagaaa
    15001 acacccgaat ggtttatgaa gcacattcta ccgattacca gacccggacg gcttattggg
    15061 aattagtccg cgatcacttt gcaatattga aagtcggtcc cgcattaacc tttgctttac
    15121 gcgaggcgat atttgcactg gcacaaattg agcaggaact tatcgccccc gaaaatcgca
    15181 gcggttgcct ggcggtaatt gaagaagtga tgctcgacga accgcaatac tggaaaaaat
    15241 attatcgcac gggttttaac gattcattac tggatattcg ttacagcctg tcggatcgta
    15301 ttcgttatta ctggccgcat agtcggatta aaaatagcgt tgaaacgatg atggtgaatc
    15361 tgcaaggcgt ggacatccca ctgggcatga ttagtcagta tcttcccaaa caatttgaac
    15421 gcattcagtc cggggaatta tcagcaatgc cgcatcagct tattatgaat aaaatttatg
    15481 atgttttgcg cgcctatcgc tacggctgtg cggaataagg acggtatatg actaacctgt
    15541 ttgttcgtag cggaatttct tttgtcgatc gtagcgaagt tttaacctat atcggtaatg
    15601 agatgctcgc gaaaggtgtg gttcatgata cctggccaca ggcattaatt gccagagaag
    15661 cagaattccc taccgggata atgcttgagc agcacgccat tgcaataccg cattgtgagg
    15721 cgattcatgc taagtcgtca gccatttatc tgttaaggcc aacaaataaa gttcattttc
    15781 agcaagcgga tgatgataac gacgtggcgg tatcgttggt tattgcgttg attgtggaaa
    15841 atccgcagca gcaattgaaa cttttacgct gtttatttgg caagttacaa cagcccgata
    15901 tcgtcgagac actaattact cttcctgaaa ctcagttaaa ggaatacttc acaaagtatg
    15961 ttttagattc agacgaataa atccctctgt aacaataaaa aggactattt atgaaacgca
    16021 agattattgt cgcttgcgga ggcgcggttg cgacctctgc gatggcggcg gaagaaatta
    16081 aagagttgtg tcagagtcat aatattcctg ttgaattaat ccagtgtcgg gttaatgaaa
    16141 tagaaaccta tatggatggt gtgcatttga tatgtaccac tgccagggtg gatcgtagtt
    16201 ttggcgatat tccgttagtt cacggcatgc cttttgtttc tggtgtcggt atcgaagcat
    16261 tacaaaataa aattctgact atcttacagg gatgacctat gttttcagaa gtcatgcgtt
    16321 atattctcga cctcggccct acagtgatgc tgccggttgt catcattatt ttttctaaaa
    16381 tattaggcat gaaggcaggc gattgcttta aagcgggtct gcatatcggg attggctttg
    16441 ttggcattgg ccttgtgatt ggcttaatgc tggattccat tggtccggcg gcgaaagcga
    16501 tggcggaaaa tttcgacctg aatctgcatg tggtcgatgt cggctggccg ggctcttcac
    16561 caatgacctg ggcgtcgcaa attgcgctgg tggcgattcc gattgcgata ttggttaacg
    16621 tggcgatgtt actgacccgt atgacgcggg tggtaaatgt tgatatctgg aatatctggc
    16681 atatgacctt caccggcgcg ttgctgcacc tggcaaccgg ttcatggatg atagggatgg
    16741 cgggtgtggt aattcacgcg gcgtttgttt ataagctcgg cgactggttt gcccgcgata
    16801 cccgaaattt ctttgagctg gaaggtattg ctattccgca cggtacgtcg gcgtatatgg
    16861 ggccgattgc ggtgctggtc gatgctatca tcgagaaaat cccaggcgtt aaccgaatta
    16921 aatttagcgc cgacgatatt cagcgcaaat ttggcccttt tggcgaacct gtcaccgtgg
    16981 gttttgtgat ggggctgatt atcggcatcc tcgcgggtta cgatgtcaaa ggtgtattgc
    17041 agctggcggt aaaaacggcg gcagttatgc tgctgatgcc acggatgatt aaacccatca
    17101 tggatggttt aacgcccatc gctaagcagg cccgtagtcg tttacaggcg aagttcggcg
    17161 gtcaggagtt cctgattggc ctggatcccg cattactgct ggggcatacg gcggtggtat
    17221 cggcaagcct gatttttatt ccgctcacca ttttaattgc tgtttgtgtt ccgggtaatc
    17281 aggtactgcc gtttggcgat cttgccacta tcggcttctt cgtggcgatg gcggtcgcgg
    17341 tgcatcgtgg aaatctgttc cgcaccttaa tctcgggtgt catcattatg agcatcaccc
    17401 tgtggatcgc gacgcaaact attggtttgc acacccaacc ggcggctaat gctggggcgt
    17461 taaaagccgg gggtatggtg gcttcaatgg atcagggcgg ttctcccatt acctggttac
    17521 tgattcaggt tttctccccg caaaatattc ccggtttcat tattatcggc gcaatttatc
    17581 tgaccggtat tttcatgacc tggcgtagag cgcgtggttt tactaaacaa gagaaagccg
    17641 ttctcgcaga ataattttta cctgaggggg aggttatccc ccttaacaa
//
LOCUS       DAJTYM010000081        17537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTYM010000081 DAJTYM010000000
VERSION     DAJTYM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            322..1005
                     /locus_tag="OO575_002851"
     CDS             322..1005
                     /locus_tag="OO575_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6665746.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1049..5711
                     /locus_tag="OO575_002852"
                     /pseudo
     CDS             1049..5711
                     /locus_tag="OO575_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6079..7734
                     /gene="lldP"
                     /locus_tag="OO575_002853"
     CDS             6079..7734
                     /gene="lldP"
                     /locus_tag="OO575_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6665747.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7734..8510
                     /gene="lldR"
                     /locus_tag="OO575_002854"
     CDS             7734..8510
                     /gene="lldR"
                     /locus_tag="OO575_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6665748.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8507..9697
                     /gene="lldD"
                     /locus_tag="OO575_002855"
     CDS             8507..9697
                     /gene="lldD"
                     /locus_tag="OO575_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6665749.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9893..10366
                     /gene="trmL"
                     /locus_tag="OO575_002856"
     CDS             9893..10366
                     /gene="trmL"
                     /locus_tag="OO575_002856"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6665750.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10419..11240)
                     /gene="cysE"
                     /locus_tag="OO575_002857"
     CDS             complement(10419..11240)
                     /gene="cysE"
                     /locus_tag="OO575_002857"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6665751.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11320..12339)
                     /gene="gpsA"
                     /locus_tag="OO575_002858"
     CDS             complement(11320..12339)
                     /gene="gpsA"
                     /locus_tag="OO575_002858"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6665752.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12339..12806)
                     /gene="secB"
                     /locus_tag="OO575_002859"
     CDS             complement(12339..12806)
                     /gene="secB"
                     /locus_tag="OO575_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6665753.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12869..13120)
                     /gene="grxC"
                     /locus_tag="OO575_002860"
     CDS             complement(12869..13120)
                     /gene="grxC"
                     /locus_tag="OO575_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6665754.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13262..13693)
                     /locus_tag="OO575_002861"
     CDS             complement(13262..13693)
                     /locus_tag="OO575_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6665755.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13938..15482
                     /gene="gpmM"
                     /locus_tag="OO575_002862"
                     /gene_synonym="pgmI"
     CDS             13938..15482
                     /gene="gpmM"
                     /locus_tag="OO575_002862"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6665756.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15492..16775
                     /gene="envC"
                     /locus_tag="OO575_002863"
     CDS             15492..16775
                     /gene="envC"
                     /locus_tag="OO575_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6665757.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16779..>17537
                     /locus_tag="OO575_002864"
     CDS             16779..>17537
                     /locus_tag="OO575_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6665758.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAV"
BASE COUNT         4399 a         4541 c         4560 g         4037 t
ORIGIN      
        1 cgtctctttt tttcggattt aattgtgaaa ttttaatcat catattcaca aaatcaatta
       61 tctgtgaaat ttttcccaga catggtttct ttcatatttg acatttatta ataatcatca
      121 ttagaatcca cgaaaaataa tatattcata caaacacaga catcttttga aacgaatgat
      181 acatttttat gatgaatgct taaaacaata tttaatcgac acacatcact gtcattaatg
      241 taaagcaaca tagctcacca ataatacaat ggacaactct accggcattg agcgctggtc
      301 ggaggaaata aggattttta catgaacttg aagaagattt ttttcagtgc tgtcactgtt
      361 tcagttctct gcgcattaac gggatgtgac tacattgaag aaggcaagcc agaaagtagt
      421 ttactgaaac aacaagaaga acataataat aaaattgtgc tacttgaaaa acaacaagca
      481 cagctaaaga gtcaactcga aactattcaa aaacaacaaa ctggcataat aaacagtaca
      541 aagactttaa ctcacgtaat taaatccgtt aaagaccaac aaaatacttt tatttttacc
      601 gagtttaacc cagcaaaaac gaagtatttc atcctgaaca acggttcggt ggctttagcg
      661 ggtcgagtgt tatccattga cgccacagag aatggtagtg ttattcatat ctcactggtc
      721 aacttattaa gtacacctat ctcaaacatt ggttttaatg cgacatgggg tggtgaaaaa
      781 cctgtcgatg ccaaagagtt tgcccggtgg caacaattac ttttcaacac atcaatgaaa
      841 tccacattga aattattacc aggtcaatgg caagacatta atttgaccct gaagggtgta
      901 tcgcccaata acctgggata tctgaagtta gccatcaaca tggaaaatat tcagttcgac
      961 aatcttccct ctgctgaaaa tcggcagaaa agaagcaaaa aataaatgca gttagtacgc
     1021 ttataacaag gaatagcgag catcattaat gaacaaaata tttaaagtta tctggaaccc
     1081 tgcgacaggg aattatactg ttaccagcga aacggcaaaa agccgtggca agaaatctgg
     1141 gcgcagtaag ctgttaattt ctgcgctggt tgcgggtgga atgttgtcgt cgtttggggc
     1201 attggcgaat gccgggaatg acaacggtca gggtgttgat tacggtagtg gatcagctgg
     1261 cgacggctgg gttgctatag gcaaaggggc gaaagcaaat acttttatga acaccagtgg
     1321 ttccagtact gctgtgggtt atgacgctat agctgaaggc caatatagct ctgccatcgg
     1381 gtcaaaaacc catgcgattg gtggtgcatc aatggccttt ggggttagtg caatatcaga
     1441 aggcgataga agtatagcac tgggtgcctc ttcgtattca ttgggccaat actcaatggc
     1501 cctcggccgt tattcaaaag cattgggtaa attgtctatt gctatggggg actcttccaa
     1561 agcggaagga gcaaacgcca ttgccctggg gcgtagcagt gtagctagcg gtactgacag
     1621 cctcgcattt ggcagacaat cacttgccag cgcagcgaac gctattgcga taggtgctga
     1681 gaccgaagcc gctgaaaatg caactgctat tggcaataat gcgaaggcaa aagggactaa
     1741 tagcatggca atggggttcg gaagccttgc cgataaagtc aatactatcg cattaggaaa
     1801 tggcagccag gctctggcag ataatgcaat cgccataggc cagggcaaca aagctgatgg
     1861 cgtggatgcc atcgctctgg gtaatggtag ccagtcgaga ggcttaaaca ccattgcctt
     1921 aggcacagcc agtaatgcaa ctggtgataa gagtcttgcg cttggtagta atagcagtgc
     1981 caacggtatt aactctgtcg cgctgggcgc agattccatt gcggatttag acaataccgt
     2041 ctctgtcggc aatagttcat taaaacgcaa gatcgttaat gtgaaaaatg gcgcgatcaa
     2101 gtctgacagt tacgatgcca ttaatggttc acagctttat gccattagcg actcggtagc
     2161 aaaaaaggct tggaggaggg gctgcagtag atgttgatga cggtactgtt acagcaccaa
     2221 cctacaattt aaaaaatggt agcaaaaata acgtaggggc tgcgctcgct gtacttgatg
     2281 aaaacaccct gcaatgggac caaaccaaag gcaaatacag cgctgctcat ggtactagta
     2341 gcccaactgc cagcgtaatc accgatgttg cggatggcac gatttcagcc tccagtaagg
     2401 atgcggttaa cggttcccaa ctgaaagcta ccaatgacga tgtcgaagcc aacaccacca
     2461 atatcaccaa cctgacggat tccgttggtg accttcaggc tgatgccctg ctctggaacg
     2521 aaactaaaaa ggcattcagt gcagctcacg gccaggatac caccagcaaa atcaccaacg
     2581 ttaaagatgc cgacctgacg gctgacagca ctgatgctgt taacggctct cagctgaaaa
     2641 ccaccaacga tgctgtggcg acgaatacca ccaatatcgc caataacact tccaatattg
     2701 ccactaacac caccaacatc tctaacctga ctgagacggt gactaatctt ggtgaggatg
     2761 cgctgaaatg ggataaggac aatggtgtat tcacggcagc tcatggcacc gagaccacca
     2821 gcaaaataac caacgttaaa gatggcgacc tgacgactgg cagcaccgat gccgttaacg
     2881 gctctcagct gaaaaccacc aacgatgccg tggcgacgaa taccaccaat atcgccacta
     2941 acaccaccaa catctctaat ctgactgaga cggtgactaa tcttggtgag gatgcgctga
     3001 aatgggataa ggacaatggt gtcttcactg cagctcatgg caacaatacc gccagcaaaa
     3061 tcaccaatat cctggacggc acagtcactg caaccagttc cgatgccatt aacggtagcc
     3121 agctttatga cttaagcagc aatatcgcca cctacttcgg cggcaatgct tctgtgaata
     3181 ctgacggtgt gtttaccggt ccaacctaca aaatcggtga aacaaattat tataacgtcg
     3241 gcgatgcact ggctgcgatt aactcctcat ttagcacgtc tctcggcgat gctctgcttt
     3301 gggatgccac cgcaggtaaa ttcagtgcca aacacggtac taatggtgac gcaagcgtga
     3361 tcactgatgt cgcagatggt gaaatttcag actccagttc tgacgcagta aacggctcac
     3421 aactccacgg cgtgagcagt tatgttgttg atgcgctggg gggtggtgcc gaagtcaatg
     3481 cagacggcac catcactgcg ccgacgtaca ccattgctaa tgctgattac gataatgtcg
     3541 gtgatgccct gaatgctatc gataccactc ctgacgacgc tctgctctgg gatgcggacg
     3601 ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga taaaactgcc agtgtaatca
     3661 ctaacgtcgc taacggtgca atctctgctg ccagcagcga cgcgattaac ggctcacaac
     3721 tctataccac caataagtac atcgctgatg cgctgggtgg tgacgcagaa gtcaacgctg
     3781 acggcaccat caccgcaccg acttacacca ttgcgaacgc cgagtacaac aacgtcggtg
     3841 acgccctgga tgcgcttgat gataacgccc tgctgtggga taagactgcc aatggcggtg
     3901 ctggagccta caatgccagc catgacggta aagccagcat catcactaat gtcgctaatg
     3961 gcagtattag tgaggacagt accgatgcag tgaacggttc tcagttgaat gcgacgaata
     4021 tgatgattga gcagaacacc caaattatca atcagctcgc tggtaacacc gacgcaacct
     4081 atatcgaaga aaatggcgct ggtatcaact acgttcgtac taacgacaac gatttagcct
     4141 ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt aggttataac gctgtcgcgt
     4201 ctggtgccag cagcgtagcc attggtcaga acagcagcag caccgttgat accggtattg
     4261 cgctgggtag cagctccgtt tccagccgtg tgattgccaa aggttctcgt gacactagcg
     4321 taacggaaaa tggcgtggtg attggttatg acaccactga cggcgaactg ctaggtgcat
     4381 tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa cgtagctgat ggttcagaag
     4441 cccatgacgc cgttacggtt cgccagttgc agaacgctat tggagcggtc gccactacgc
     4501 caaccaagta ctttcacgcc aactcaacgg cagaagactc actggccgtt ggtgaagact
     4561 cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc aggtattggt atcggtttga
     4621 acactctggt tctggctgat gcgatcaacg gcattgctat cggcagtaac gcacgcgcaa
     4681 accatgcaaa cagtatcgca atgggtaacg gttctcagac cacccgtggt gcacagactg
     4741 gctacaccgc ctacaacatg gacgcaccgc agaactctgt aggtgagttt tctgtcggta
     4801 gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg ttcagcggat accgatgcgg
     4861 ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc gcaaaatacc cagagcatta
     4921 ctaacctgaa caatcaggtc actaatctgg atactcgcgt tactaatatc gaaaacggta
     4981 ttggcgacat tgtcaccacc ggtagcacca agtacttcaa gaccaacacc gatggcgtag
     5041 atgccaacgc ccagggtaaa gatagcgttg ctattggttc tggttccatt gctgccgctg
     5101 acaacagcgt cgcactgggt accggttccg ttgcagagga agaaaataca atctctgtag
     5161 gttcttctac taaccaacgc cgtatcacca acgtagctgc cagtgttaat gccaccgatg
     5221 cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg agtgcgctac gacaccaaag
     5281 ctgatggttc tatcgactat agcaatatca ccctcggtgg tggcaacggc agtacgactc
     5341 gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc ggtgaactac gcgcagttga
     5401 agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg gatggttgag atggataaca
     5461 aactgtctaa aactgaaagc aagttgagcg gtggtatcgc ttctgcaatg gcaatgaccg
     5521 gtctgccgca ggcttataca ccgggtgcca gcatggcttc tattggtggc ggtacttaca
     5581 acggtgaatc ggcagttgct ttaggtgtat cgatggtgag cgccaatggt cgttgggtct
     5641 acaaattaca aggtagtacc aatagccagg gtgaatactc cgccgcactc ggtgccggta
     5701 ttcagtggta atgatccatt aagttagtgt gactaagggc aaacgccaaa ttgcctgatg
     5761 cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac aatctaaggg caattctctg
     5821 atgaggattg cccttttctt taccagacat ctccccccac aagaattggc cctaccaatt
     5881 cttcgcttat ctgacctctg gttcacaatt ccccaattaa aactcacatc aatattgcca
     5941 atacataaca tttagttaac cattcattgt cattatccct acacaacaca attggcagtg
     6001 ccacttttac acaacgtgtg acaaggagat gagcaacaga ctcattacac gatgtgcgtg
     6061 gactctagga gacctgcaat gaatctctgg caacaaaact acgatcccgc cgggaatatc
     6121 tggctttcca gtctgatagc atcgcttccc atcctgtttt tcttctttgc gctgattaag
     6181 ctcaaactga aaggatacgt cgccgcctca tggacggtag caatcgccct tgccgtggct
     6241 ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct cggtggttta tggcttcttc
     6301 tatggtttgt ggcccatcgc gtggatcatt attgcagcag tgttcgtcta taagatctcg
     6361 gtgaaaaccg ggcagtttga catcattcgc tcgtctattc tttcgataac ccctgaccag
     6421 cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg agccgcaggc
     6481 tttggcgcac cggtagcaat taccgccgca ttgctggtcg gcctgggttt taaaccgctg
     6541 tacgccgccg gactgtgcct gattgttaac accgcgccag tggcatttgg cgcgatgggc
     6601 attccaatcc tggttgccgg acaagtaaca ggcatcgaca gctttgagat tggccagatg
     6661 gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt tctggatcat ggcgattatg
     6721 gacggctggc gcggtatcaa agagacgtgg cctgcggtcg tggttgcagg cggctcgttt
     6781 gccatcgctc agtaccttag ctctaacttc attgggccgg agctgccgga cattatctct
     6841 tcgctggtat cactgctctg cctgacgctg ttcctcaaac gctggcaacc agttcgcgta
     6901 ttccgcttcg gtgacttggg ggcgtcacag gttgatatga cgctggccca caccggttac
     6961 accgcgggtc aggtgttacg tgcctggaca ccgttcctgt tcctgaccgc taccgtaaca
     7021 ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg gtggtgcgct gtatgagtgg
     7081 gtgatcaaca ttccggtgcc gtacctcaat aaactggttg cccgtatgcc accagtggtc
     7141 agcgaggcta cagcctatgc cgccgtgttt aagtttgact ggttctctgc caccggcacc
     7201 gccattctgt ttgctgcact gctctcgatt gtctggctga agatgaaacc gtctgacgct
     7261 atcagcacct tcggcagcac gctgaaagaa ctggctctac ccatctactc catcggcatg
     7321 gtgctggcgt tcgcctttat ctcgaactat tccggactgt catcaacact ggcactggca
     7381 ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt tcctcggctg gctgggggta
     7441 ttcctgaccg ggtcggatac ctcatctaac gccctgttcg ccgcgctgca agccaccgca
     7501 gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg ccaataccac cggtggcgtc
     7561 accggtaaga tgatctcccc gcaatctatc gctatcgcct gtgcggcggt aggcctggtg
     7621 ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca gcctgatctt cacctgtatg
     7681 gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt ggatgattcc ttaatgattg
     7741 ttttacccag acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg attgatgaaa
     7801 aaaacctgga agcgggcatg aagttgcccg ctgagcgcca actggcgatg caactcggcg
     7861 tatcacgtaa ttcactacgc gaggcgctgg caaaactggt gagcgaaggc gtgctgctca
     7921 gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga cacatggtcg gagcaaaaca
     7981 tcgtccagcc gctaaaaacg ctgatggccg atgatccgga ttacagtttc gatattctgg
     8041 aagcccgcta cgccattgaa gccagcaccg catggcatgc ggcaatgcgc gccacacctg
     8101 gcgacaaaga aaagattcag ctttgctttg aagcaacgct aagtgaagac ccggatatcg
     8161 cctcacaagc ggacgttcgt tttcatctgg cgattgccga agcctcacat aacatcgtgc
     8221 tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc ctcagtgaag catagccgtc
     8281 agcggatgta tctggtgcca ccggtttttt cacaactgac cgaacaacat caggctgtca
     8341 ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa agcaatgatg gcgcacctta
     8401 gttttgttca caccaccatg aaacgattcg atgaagatca ggctcgccac gcacggatta
     8461 cccgcctgcc cggtgagcat aatgagcatt cgagggagaa aaacgcatga ttatttccgc
     8521 agccagcgat tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt tccactatat
     8581 ggatggtggt gcatattctg aatacacgct gcgccgcaac gtggaagatt tgtcagaagt
     8641 ggcactgcgc cagcgtattc tgaaaaacat gtccgactta agcctggaaa cgacgctgtt
     8701 taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg ggtttgtgtg gcatgtatgc
     8761 gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg catggcattc cgtttactct
     8821 ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc gcccaatgtg
     8881 gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa
     8941 agcagcgggt tgttcgacgc tggttttcac cgtggatatg ccgacaccgg gcgcacgcta
     9001 ccgtgatgcg cattcaggta tgagcggccc aaatgcggca atgcgccgct acttgcaggc
     9061 ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac atgatttagg
     9121 taatatctca gcttatctcg gcaaaccgac cggactggaa gattacatcg gctggctggg
     9181 gaataacttc gatccgtcca tctcatggaa agaccttgag tggatccgcg atttctggga
     9241 tggcccgatg gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg cagtacgttt
     9301 tggtgctgat ggaattgtgg tttctaacca cggtggccgc cagctggacg gtgtactctc
     9361 ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatcg ccattctggc
     9421 ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac
     9481 cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg cgggtgtagc
     9541 taacctgcta aatctgatcg aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa
     9601 atcgatcagt gaaattacgc aagattcgct ggtgcagggg ctgggtaaag agttgcctac
     9661 ggcactggct ccgatggcga aagggaatgc ggcatagtcg tttgcgcccc ctcaccctaa
     9721 ccctctccct cagggagagg ggaccgttcg gcgctgtatg tactccctca ctctgaaacg
     9781 acaccgcact ctttttttct ccctcgcccc tccggggagg gccggggtga ggggaaaagg
     9841 ccgcacggtt caccaatttt ctgctatcct gcccccgcac taagggggca gtatgctaaa
     9901 catcgtactt tacgaaccag aaattccgcc aaatactggc aacatcatcc gtctttgcgc
     9961 taataccggc tttcgtctac atatcatcga accgatggga tttgcctggg acgacaagcg
    10021 cctgcgccgc gcggggctgg actatcacga gtttaccgcc gttacgcgtc atcatgacta
    10081 tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt gccctcacca cgaaaggtac
    10141 gcctgctcac agcgccgtaa gctatcagga tggcgattat ctgatgttcg gcccggaaac
    10201 acgcggcctg ccagcgagca ttcttgatgc cctgcccgct gaacaaaaaa ttcgcattcc
    10261 gatggtgccg gacagccgca gcatgaatct gtccaatgcg gtgtcggtag tggtgtatga
    10321 agcctggcgg cagctgggat atccgggagc gttgttgaga gattagttac tgtatgccgg
    10381 atagatgatt acatcgcatc cggcacgatc acaggacatt agatcccatc cccatactca
    10441 aatgtatggt taataccgtt gaaatgctgg tccatatcca ttgatggctt atcgctgtct
    10501 ggtttaccga caatacgagc cggaacgcca gcggcggttg tatgcggcgg caccggttgc
    10561 agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa cttcaatatt gccgaggatt
    10621 ttcgcgcccg cgccaatcat cacaccttca cgaattttcg ggtgacggtc accaccagat
    10681 ttacccgtac cgccaagcgt cacagattgc agaatcgata cgtcgttttc aattaccgcc
    10741 gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa tgccacggcc aatttttgct
    10801 gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt tttgcagaaa gattgccaat
    10861 gcgcgacgcc cctgattcca caaccagtga ccgatgcgat aggcctgcaa ggcatgaaaa
    10921 cccttcaggt ataacaacgg ggttgagtat ttatcgactg ccgggtcgcg ggtacgcacc
    10981 gcctgaatat cacaggccgc agaggcgatc atttccgggt cagcggcgta ggcttcttca
    11041 accacttcac ggatagcaat agcaggcata attggcgatg acagcttgtt cgccagcatg
    11101 tagctcagtg cactgccaag gttttcgtgc ttgagtagcg tcgcgtggta aaaactggcc
    11161 agcattggct cacagtccgc cagcgttctg gcttcggctt taatattgtt ccagacaatt
    11221 tccagttctt cacacgacat tgcttactcc acacgatgag ataatgaccg gcccgttctg
    11281 cgcgggccgg gtcatagcgg taacaaaggt tccctggggt tagtgactgc tgcgctcgtc
    11341 cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc gcgttttttc cgcaatataa
    11401 tacttgataa atttcctcgg ttattggcat ttcaacgccg aagcgatgcg ccagttcgcg
    11461 gacttctttc gtattgcggt agccttccac cacctgacca atcttctcct gcgcgctttg
    11521 tacatccatg ccctgaccga gcatcatacc aaaacggcgg ttacgcgact ggttgtcggt
    11581 acaggtaagc accagatcgc ccagccccgc catgcccata aaggtggcag gatcggcacc
    11641 cagcgccgca ccaagacgcg acatttcagc cagcccacgg gtgatcagcg ccgtacgcgc
    11701 attcgcacca aaaccgatac cgtcagacat ccccgcgcca atggcaatga cgtttttcac
    11761 cgcgccgcca agctgcacgc caatgaaatc cgggttgctg taaacgcgga aacttttgcc
    11821 gcagtgcagc agctgctgga gatcatcggc aaaggtctga tcggtcgagg ccagcgaaat
    11881 agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt gggccagaga taaccgccag
    11941 cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt aacagacgtc cggtttccgc
    12001 ttccagccct ttggtcgccc acaccagacg cgcatcagga cgcatcagcg gtttaatctg
    12061 gcgcagcact tcaccaaaga catggctggg tacgacgacg agaatattac gactggctgc
    12121 cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg ggaaaaggca catcggggag
    12181 aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata tgttcagggt catggcccca
    12241 gaggacaacc tcgtggccat ttcttgccag ggtaatggca agagcggtgc cgtacgagcc
    12301 ggcaccgatc acagtcattg aagcattacg ttggttcatc aggcatcctg atgttcttca
    12361 gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca gcgcatcgaa gttaaccggc
    12421 gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc tggtgatgca ctcacgagca
    12481 tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat gtgccatctg ggtgccttcg
    12541 atacccgcga tggagaaaat accgccctgc tgaacttcac acaggaacgc ggtttcttcg
    12601 cccaaggagg ccgttacggt aacacgcagt accacttcgt atacgtcatc tgccagttgg
    12661 gaagatgccg tatccagatc aagtttaact tctggttgcc aatctttctg gaaaacgtgc
    12721 ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa tacgttggat ctggaaagtc
    12781 atttcagtgt tgttttgttc tgacatgtgt agaaaaccct taagtgttgt ccttaaatac
    12841 tgcgcaatgt aataccaacg ttcacacgtt atttcagcag gggatccagt ccaccacgtg
    12901 catccaatgc atacaagtca tcacagccgc caatgtgctg tgcgtcaata aaaatctggg
    12961 gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg cttggcggca ttgccatcga
    13021 tcggcaactc ctggaaactc acgcccttgc tgctcagcag tgcttttgca cgatggcaat
    13081 acgggcaggt ttctttggta tagatttcaa cattggccat aatttagctc ctgttttttt
    13141 accctgaaat ttcatgttgc agggcggctg caaaatggag tgtccccaga agcttataac
    13201 aataagtcgc tgggggccta attgcagcta acggcctgca tcatgaaaga cgacaggtaa
    13261 attatttgcc gcgcaccaga ggcaagtttt cgccagccca gccagcaacg ccctctttca
    13321 gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca
    13381 tgccagaacc gtctaccacg ataaccgatt tgtctttgtg cttctcaagc tcaccaacat
    13441 tgttggcttt gatttcgctc ggcaacagat tgatagaacc tgcgatatgg cctttacgga
    13501 agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc agacgcgtag
    13561 cttcaccacg agtaatcacc ttcactttcg aggtcaggct cttaaacgta gtcacaagaa
    13621 ccgccaccag taacgcgatc caggcgatac tcagtatggg atgacggcca acaaattgca
    13681 taatttcttg catggggggt aacaactccc gacgtagtga ttaaaaaaac caggaaagga
    13741 gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca attttgcggg
    13801 gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc gcgagcggtc gtatattttg
    13861 atcttcagag gctattttat cgattcagct gtagtaaaat tacgcaaatt ttgactcttg
    13921 agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg attctggatg
    13981 gctatggcta tcgtgaagaa cagcaggata acgccatttt tagtgctaaa accccggtaa
    14041 tggatgcact gtgggccaat cgtccgcata ccctgatcga cgcttccggt ctggaagtcg
    14101 gtctgcctga ccgtcagatg ggtaactccg aagtaggcca cgttaacctg ggtgccggcc
    14161 gcatcgtgta tcaggacctg actcgtctgg acgttgaaat caaagatcgc gctttctttg
    14221 ctaatccagt gctggctggc gcggtacata aagcgaaaaa cgcaggtaaa gcagtacaca
    14281 ttatgggtct gctctccgca ggcggcgtac acagccacga agatcacatc atggcgatgg
    14341 tagaactggc agctgaacgc ggcgcagaaa aaatctacct gcacgcattc cttgacggtc
    14401 gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa atttgaagag aaatttgctg
    14461 cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata
    14521 accgttggga tcgcgttgaa aaagcctatg acctgctgac tctggcgcag ggtgagttcc
    14581 aggccgatac tgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa aacgacgagt
    14641 tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg
    14701 atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact cgtgcattcg
    14761 tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc
    14821 tgaccgaata cgccgctgac atcaaaactg cggttgctta cccacccgct tccctggtta
    14881 acaccttcgg cgagtggatg gcgaaaaacg acaaaactca gttgcgtatt tccgaaaccg
    14941 aaaaatatgc ccacgttact ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg
    15001 aagatcgcat tctgatcaac tcaccgaaag tggctaccta cgatctgcaa ccggaaatga
    15061 gctccgcaga gctgaccgaa aaactggttg cggccatcaa gagcggcaaa tacgacacca
    15121 ttatctgtaa ctatccgaac ggcgacatgg taggtcacac cggggtgatg gaagcggcgg
    15181 ttaaagcggt tgaagcactg gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg
    15241 tgggtggaca actgctgatc accgctgacc acggtaacgc tgaacagatg cgcgatccgg
    15301 caacgggtca ggcacacacg gcacacacca acctgccagt tccgctgatt tacgttggtg
    15361 ataagaacgt gaaagcggtt gcaggcggca aactttctga catcgcgccg accatgttgt
    15421 cgctgatggg tatggaaatc ccgcaagaga tgactggtaa gccgctgttc atcgtggaat
    15481 aatccctccc catgagggga aaggcgatta ataccatgac acgggccgtg aaaccgcgca
    15541 ggtttgcaat caggcccatc atctacgcca gcgttcttag cgctggcgta ttgttgtgcg
    15601 ccttttccgc ccacgcggat gagcgtgacc aactcaaatc tattcaggcc gatatcgccg
    15661 caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc gcacaattga
    15721 aaaagcagga agaagcgatc tctgaagcca cccgtaagct gcgcgaaacg caaaacacgc
    15781 tcaatcaact caataaacag attgatgaga tgaacgcgtc gattgccaaa ctggagcagc
    15841 aaaaagccgc ccaggagcgc agcctcgccg cacaactgga tgccgcattc cgtcagggcg
    15901 agcataccgg tattcagcta attctcagcg gtgaagaaag ccagcgtgga cagcgtttac
    15961 aggcttattt cggctatctc aaccaggcgc gacaagaaac cattgcccag ttgaagcaaa
    16021 cgcgtgaaga agtcaccatg cagcatgctg aactggaaga gaaacaaagc gagcaacaaa
    16081 cgctgttata tgaacagcgc gcccaacagg cgaaactgac tcaggctctg aacgagcgta
    16141 aaaagacgct ggcggggctg gaatcttcca tccagcaagg tcagcagcag ctaagtgaac
    16201 tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg tgctgaagcc gcggcgaaag
    16261 cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg cgagcgccag aaagaagcga
    16321 cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa atcgctgatg tcccgaactg
    16381 gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt tcgcgggccg acgctgcatc
    16441 gctatggcga gcagctacag ggtgaattac gctggaaagg gatggttatc ggtgcctctg
    16501 aaggtactga agttaaagca attgccgacg gtcgagtgat tctggctgac tggctgcaag
    16561 gttacggtct ggtggtggtg gttgagcacg gtaaaggcga catgagtctt tacggctata
    16621 atcagagcgc actggtgagc gttggttcgc aggttcgcgc gggccagcca attgcactgg
    16681 tgggcagcag tggcggtcag ggtcggcctt cactctattt cgaaattcgc cgccagggtc
    16741 aggcggtcaa tccacagccg tggttgggaa gataagtttt gtttccattt cgtcgtaacg
    16801 ttcttgcatt tgccgctctg ttggcgctct cctcccccgt acttgctggc aaacttgcca
    16861 tcgtcattga tgattttggg tatcgcccgc acaacgaaaa ccaggtgctg gcgatgcctt
    16921 ccgctatctc cgtcgctgta ttacccgatt caccgcacgc cagagagatg gcgaccaaag
    16981 cgcataacag cggtcacgaa gtgttgattc atctcccgat ggcaccgttg agtaaacagc
    17041 cgctggagaa aaatacgcta cgcccggaga tgagcagcga cgaaattgag cgcattattc
    17101 gtagtgcggt caataacgtg ccctatgccg tggggatcaa caaccacatg ggcagcaaga
    17161 tgacctctaa cctgtttggt atgcagaaag tgatgcaggc gctggagcgt tacaatcttt
    17221 acttcctcga cagcgtaacc atcggtaata cccaggcgat gcgcgccgcg caaggtactg
    17281 gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc gcagaacgaa gcggacatcc
    17341 gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa cggttcaacc attgccattg
    17401 ggcatcctca cccttcaacg gtacgcgtgc tacaacagat ggtttataac ctgccgccag
    17461 acattacgct ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat acttctacac
    17521 cgccgaaaaa cgctgtg
//
LOCUS       DAJTYM010000082        17257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTYM010000082 DAJTYM010000000
VERSION     DAJTYM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="OO575_002865"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="OO575_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6665759.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO575_002866"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO575_002866"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6665760.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO575_002867"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO575_002867"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6665761.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="OO575_002868"
     CDS             complement(2205..2348)
                     /locus_tag="OO575_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6665762.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3019..3306)
                     /locus_tag="OO575_002869"
     CDS             complement(3019..3306)
                     /locus_tag="OO575_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6665763.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO575_002870"
     CDS             complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO575_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6665764.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3683..3922
                     /locus_tag="OO575_002871"
     CDS             3683..3922
                     /locus_tag="OO575_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665765.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO575_002872"
     CDS             complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO575_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6665766.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5033..6406
                     /locus_tag="OO575_002873"
     CDS             5033..6406
                     /locus_tag="OO575_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6665767.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO575_002874"
     CDS             complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO575_002874"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6665768.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO575_002875"
     CDS             complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO575_002875"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6665769.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO575_002876"
     CDS             complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO575_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6665770.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO575_002877"
     CDS             complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO575_002877"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6665771.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11015..12298
                     /gene="yebS"
                     /locus_tag="OO575_002878"
     CDS             11015..12298
                     /gene="yebS"
                     /locus_tag="OO575_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6665772.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12267..14887
                     /gene="yebT"
                     /locus_tag="OO575_002879"
                     /pseudo
     CDS             12267..14887
                     /gene="yebT"
                     /locus_tag="OO575_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14967..16406
                     /gene="rsmF"
                     /locus_tag="OO575_002880"
                     /pseudo
     CDS             14967..16406
                     /gene="rsmF"
                     /locus_tag="OO575_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16524..16760
                     /locus_tag="OO575_002881"
     CDS             16524..16760
                     /locus_tag="OO575_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6665773.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16865..17056
                     /locus_tag="OO575_002882"
     CDS             16865..17056
                     /locus_tag="OO575_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6665774.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(17081..>17257)
                     /locus_tag="OO575_002883"
     CDS             complement(17081..>17257)
                     /locus_tag="OO575_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6665775.1"
                     /translation="QKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDI
                     GNLHYIDTGAVFGG"
BASE COUNT         4185 a         4351 c         4161 g         4560 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
     2821 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
     2881 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
     2941 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
     3001 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
     3061 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
     3121 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
     3181 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
     3241 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
     3301 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
     3361 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
     3421 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
     3481 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
     3541 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
     3601 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
     3661 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
     3721 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     3781 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     3841 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     3901 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     3961 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     4021 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     4081 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     4141 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     4201 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     4261 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     4321 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     4381 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     4441 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     4501 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     4561 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     4621 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     4681 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     4741 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     4801 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     4861 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     4921 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     4981 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     5041 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     5101 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     5161 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     5221 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     5281 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     5341 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     5401 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     5461 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     5521 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     5581 cctgcttctg gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     5641 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     5701 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     5761 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     5821 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     5881 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     5941 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     6001 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     6061 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     6121 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     6181 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     6241 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     6301 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     6361 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     6421 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     6481 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     6541 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     6601 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     6661 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     6721 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     6781 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     6841 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     6901 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     6961 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     7021 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     7081 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     7141 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     7201 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     7261 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     7321 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     7381 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     7441 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     7501 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     7561 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     7621 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     7681 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     7741 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     7801 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     7861 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     7921 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     7981 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     8041 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     8101 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     8161 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     8221 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     8281 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     8341 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     8401 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     8461 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     8521 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     8581 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     8641 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     8701 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     8761 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     8821 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     8881 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     8941 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     9001 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     9061 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     9121 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     9181 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     9241 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     9301 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     9361 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     9421 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     9481 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     9541 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     9601 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     9661 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     9721 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     9781 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     9841 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     9901 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     9961 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
    10021 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
    10081 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
    10141 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
    10201 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
    10261 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
    10321 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
    10381 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
    10441 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
    10501 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
    10561 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
    10621 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
    10681 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
    10741 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
    10801 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
    10861 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
    10921 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
    10981 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
    11041 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
    11101 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
    11161 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
    11221 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
    11281 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
    11341 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
    11401 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
    11461 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
    11521 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
    11581 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
    11641 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
    11701 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
    11761 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
    11821 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
    11881 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
    11941 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
    12001 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
    12061 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
    12121 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
    12181 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
    12241 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
    12301 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
    12361 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
    12421 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
    12481 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
    12541 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
    12601 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
    12661 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
    12721 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    12781 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    12841 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    12901 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    12961 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    13021 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    13081 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    13141 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    13201 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    13261 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    13321 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    13381 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    13441 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    13501 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    13561 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    13621 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    13681 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    13741 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    13801 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    13861 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    13921 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    13981 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    14041 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    14101 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    14161 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    14221 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    14281 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    14341 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    14401 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    14461 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    14521 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    14581 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    14641 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    14701 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    14761 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    14821 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    14881 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    14941 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    15001 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    15061 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    15121 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    15181 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    15241 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    15301 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    15361 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    15421 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    15481 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    15541 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    15601 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    15661 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    15721 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    15781 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    15841 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    15901 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    15961 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    16021 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    16081 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    16141 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    16201 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    16261 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    16321 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    16381 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    16441 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca
    16501 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    16561 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    16621 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    16681 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    16741 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    16801 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    16861 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    16921 tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg
    16981 ccatggcagt tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga
    17041 gtatggtgat ttttaattat tgcagttgca caagagtcag ttagccccca aagacagcac
    17101 cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa
    17161 accagaaatg atcagcacct gtaattccct gcccttttgg gcgttcacct aatcgcgagc
    17221 ggctccacaa gacctgatgc aaatcaacgt ccttttg
//
LOCUS       DAJTYM010000083        17024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTYM010000083 DAJTYM010000000
VERSION     DAJTYM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..487)
                     /gene="chbR"
                     /locus_tag="OO575_002884"
     CDS             complement(<1..487)
                     /gene="chbR"
                     /locus_tag="OO575_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6665776.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YR"
     gene            complement(495..845)
                     /gene="chbA"
                     /locus_tag="OO575_002885"
     CDS             complement(495..845)
                     /gene="chbA"
                     /locus_tag="OO575_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6665777.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(904..2252)
                     /gene="chbC"
                     /locus_tag="OO575_002886"
                     /pseudo
     CDS             complement(904..2252)
                     /gene="chbC"
                     /locus_tag="OO575_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2337..2657)
                     /gene="chbB"
                     /locus_tag="OO575_002887"
     CDS             complement(2337..2657)
                     /gene="chbB"
                     /locus_tag="OO575_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6665778.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2956..3294)
                     /gene="osmE"
                     /locus_tag="OO575_002888"
     CDS             complement(2956..3294)
                     /gene="osmE"
                     /locus_tag="OO575_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6665779.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3496..4323
                     /gene="nadE"
                     /locus_tag="OO575_002889"
     CDS             3496..4323
                     /gene="nadE"
                     /locus_tag="OO575_002889"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6665780.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4553..5440
                     /gene="cho"
                     /locus_tag="OO575_002890"
     CDS             4553..5440
                     /gene="cho"
                     /locus_tag="OO575_002890"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6665781.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5400..5975)
                     /gene="ves"
                     /locus_tag="OO575_002891"
     CDS             complement(5400..5975)
                     /gene="ves"
                     /locus_tag="OO575_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6665782.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6178..6663)
                     /gene="spy"
                     /locus_tag="OO575_002892"
     CDS             complement(6178..6663)
                     /gene="spy"
                     /locus_tag="OO575_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6665783.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6993..7961)
                     /gene="astE"
                     /locus_tag="OO575_002893"
     CDS             complement(6993..7961)
                     /gene="astE"
                     /locus_tag="OO575_002893"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6665784.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7954..9287)
                     /gene="astB"
                     /locus_tag="OO575_002894"
                     /pseudo
     CDS             complement(7954..9287)
                     /gene="astB"
                     /locus_tag="OO575_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9284..10762)
                     /gene="astD"
                     /locus_tag="OO575_002895"
     CDS             complement(9284..10762)
                     /gene="astD"
                     /locus_tag="OO575_002895"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6665785.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10759..11793)
                     /gene="astA"
                     /locus_tag="OO575_002896"
     CDS             complement(10759..11793)
                     /gene="astA"
                     /locus_tag="OO575_002896"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6665786.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11790..13010)
                     /gene="astC"
                     /locus_tag="OO575_002897"
                     /pseudo
     CDS             complement(11790..13010)
                     /gene="astC"
                     /locus_tag="OO575_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13456..14262
                     /gene="xthA"
                     /locus_tag="OO575_002898"
     CDS             13456..14262
                     /gene="xthA"
                     /locus_tag="OO575_002898"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6665787.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14429..15139
                     /locus_tag="OO575_002899"
     CDS             14429..15139
                     /locus_tag="OO575_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6665788.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15144..15821
                     /gene="ydjY"
                     /locus_tag="OO575_002900"
     CDS             15144..15821
                     /gene="ydjY"
                     /locus_tag="OO575_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6665789.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15836..16543
                     /locus_tag="OO575_002901"
     CDS             15836..16543
                     /locus_tag="OO575_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6665790.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16543..>17024
                     /locus_tag="OO575_002902"
     CDS             16543..>17024
                     /locus_tag="OO575_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6665791.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     A"
BASE COUNT         4217 a         4535 c         4155 g         4117 t
ORIGIN      
        1 cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac catctcaaca aactcttcca
       61 gccctgtttc gcggaaattt aatgaagaaa tcactgtttc cacataggtg agaaaagcgt
      121 tattggtccg gtatacctgc gaagcaacaa agcaataagg caacaacggc aggtaatgct
      181 gctcaaaaaa gcgtttactg atcccaacgt tcaatatgcg cgtggcacca aactcataaa
      241 aactttgatg gtgcgaacct aacggaataa aaacaaaatc gccccgttcc agtaacacgc
      301 gcttaccgtt aatctcctgg aaataacgcc cggttaatac cagagtaaat tcataatagt
      361 cgtgctgatg cagtccgctg atactctcag ttttgttata gataaacaca tggaagtttt
      421 tgccattaaa caactgctgt tctcgggcag tggcaatttc cggcgcgtta atcactggct
      481 gcatcatcga ctccttatgc cttcagtttt tcatgaagct caattaattc agtaatcagt
      541 tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt ggaccagcac cagactcact
      601 ttcatcttac cttcgcccgc atcgccttca atcagtttcg tctgtaccag atgcgcttca
      661 ttcaatgcca tccgtgactg atccatcatg gctttcgctg cggcaaaatc gccctgcttc
      721 gcctgtttca atgcggcata cgccaggcta cgcgcttgtc cagagttgat gataagcccc
      781 atcaccactt cttccagctc ttcagcttct gtttgcgtat caggaatatt atcgagatcc
      841 atcatacatc gttcctctct cttcttaccg gcaagattac ccgtaccggc atcgattaaa
      901 atttcagggg ttagcgatat cttcttcgct ctcttcttta tcaatcgcat tttgtgcttt
      961 gttagccacc acaacaaagg gcagataaat taacgttgcg atgccaaggt tgaagagtgc
     1021 gaccagcaat gcggcgacgc taccgttggt gttaaagaag gctcccagac cggttggcat
     1081 ggtccacggt gcaatattgg tcaccggagg aatgatgccc atgtagtacg ctgcgagggt
     1141 gattgccgcc agaatcggtt gtaccagtac aaacgggata aacatcaccg ggttcatgat
     1201 aattggcaga ccaaacagaa tcggttcgtt aatctggaag atgccggacg gcagcgccag
     1261 ttttgccacc tgacgataat cagcacggcg agaggcgata aagatagcca ggatcaggcc
     1321 taaagtcgca ccactgcccc caaggaaaat aaaggagtcc agcatcggct tggcccagat
     1381 atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa ccatattgct gataggtcgc
     1441 gatattttcc agcgcccacg gcgtcataat gccgttgtcc agtgcggtca gcgccagcgc
     1501 gccatgaata ccgaagaacc agagcagtgg aacaaagatc acataggccc agcccaccac
     1561 gctacccaac gatgccagtg gggttgagat ggtatccata atgatctgat ggaagttggt
     1621 gccccaggta ttcaacgccc aggcaataat ccccatcacg gaaagaataa taaagccggg
     1681 aattaatgcc gagaaagaac gcgatactga agcaggtacg ctgtcgggca gtttaatgac
     1741 ccaattgcgg cggacaataa aggtaaacat ttctgccacc accaggccaa taataatccc
     1801 ggagatgata ttcgccccac ctaaccagtt tgcgccaacc gcataggcct caccgacact
     1861 atatggggtg acggtcataa atgctgcaac ggataacaac ccagccgcca gcgcatcgac
     1921 tttacgctct tctgccagcg ccatgccaat aaagaacggt gccattaaag acattattcc
     1981 taatgttccg ttatatacgt tgccgccaat acctttcaga ccattaagtg tttcaatggt
     2041 tgaggcgtcg aggcgaatac ctaaggaata aaaaacgacc cctccccaaa gcttagaaaa
     2101 acgttgttaa ttaatacaaa catggccccc gcaagggtta acggcattaa gcgaataaag
     2161 ccatttttga ttgcattaac gtgtggctgc tttcctattt taactgcaaa agggaggagt
     2221 accttttcaa gcgatgcaat aacattactc atagaaaaat acccttaaaa accgcaattt
     2281 aaatattacg gtattgattt atgaaataac tctttgacgg gaaaatttaa aataaattaa
     2341 tttgctgcgg cttttttaat cgctgcaacc gcagccttaa gcacgcctaa accatcgact
     2401 ttgccataaa gcagcgagtc aattacttca accggtttgt tgggtaacaa acgctggatt
     2461 tcgggcaaca tataagcaat ctgcggccct aataacacga catcggcatt ctgacctttt
     2521 tcaccagcca gtgtttccgg aaatgcttca ataatgaccg gaacttcata tttttctgcc
     2581 tgtgcgcgca tttttgatac cagtaaagag atagacatgc ccgcagaaca aaacagataa
     2641 atgtgtttct tttccataaa actgccctca tcgacgatta tctgtcagcc agacactccg
     2701 caagccttga cctgcttcca tgctctgggt aacttgcgaa accaacatga ctttttttgt
     2761 ggctgaaagg agtatacggt atagacctga gcaatagtat ttctttgacc ttataaattg
     2821 tttctccttg acctgtggtt atgaccctct tcacatttcg ggcaaataat tcgcgaagat
     2881 aattaagaac tcaggccaaa aaaaaccggc gcaatggccg gtttccgttg ttactcaagc
     2941 tttcagacga attgattact tcgcagcctg tggatcagtg tcgtattcag cacaggtctg
     3001 ataaccggag ttgatgacat gtccggtatc atctaacgcg acaaagtagg tttctgcttt
     3061 accatcacgt tgacccagga tgtaggtctg gcaagtaccg cgagcatgga tcatgctcac
     3121 ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc cggctcatgc cttttttgac
     3181 gtctttcacc acaggctgta caaactggtc tttggtacga tcataagctg tacaacccgc
     3241 cagcatggtt aataccgccg ctgcactcag aattcctgcc atattcttgt tcatattccg
     3301 tcctcttgtt tatcagcgtg ttagataagc ctggaataca ttgggcgctt tttcaagccc
     3361 gtgaacgaaa cggctccgct ttcagaggat tcctgtatga cgttttaacc accattcagc
     3421 ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt aaggaagttt tgtcttttct
     3481 gtctggaggg gttcaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg
     3541 cagattaatg ctgaagagga aattcgtcgt agtgtcgatt ttctgaaaag ctacctgcaa
     3601 acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt
     3661 gccggaaagc tgtgccagat ggcgattaat gagctgcgcc aggaaactcg caacgaatca
     3721 ctgcaattta ttgccgtacg cctgccctat ggtgttcagg ctgacgaaca agattgccag
     3781 gatgccattg cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta
     3841 ttggctagcg agcaggcatt gcgggaagca ggcattgaac tgagcgattt tgtccgtggc
     3901 aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt
     3961 gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg gattcttcac taaatatggt
     4021 gacggcggta cggatattaa tccgctgtat cgtctcaaca aacgccaggg taaacagtta
     4081 ctgacggcat taggttgccc ggaacacctt tataagaaag ccccaactgc cgatcttgaa
     4141 gatgatcgcc cttctctgcc agatgaagtg gcactcggcg tgacctatga caatatcgac
     4201 gactatctgg aagggaaaaa cctacctgaa caggtcgcca gaacaataga gaactggtat
     4261 ctgaaaaccg aacataaacg ccgtccgcca attaacgttt tcgatgattt ctggaaaaag
     4321 taataatttg cacctattgg attgtgcgaa aaagagtaat ttgttcacgc cggatgcggc
     4381 gtgaacgcct tattctacct acaaaactat gcaaattcaa tacattgcag gagtcgaata
     4441 ggcctgacag gcgtagcacg tcagacggtg taacctttgt catcaacccg cctctttttt
     4501 aatcgcctcc cgcctgttac actggataga taaccagcat tcggagtcaa cagtggtacg
     4561 gcgtttaact tctccgcggc tcgaatttga agctgcggca atttatgaat atcccgaaca
     4621 tttacattca ttccttaatg acttacccac ccgaccaggg gtgtatctgt ttcatggcga
     4681 aagtgacacc atgccgctct atatcggcaa aagcgttaat atccgcagtc gggtgctttc
     4741 tcatttacgc accccggatg aagccgccat gctacggcaa tcccggcgaa ttagctggat
     4801 ctgcaccgca ggcgaaatcg gcgctctgct ccttgaagcg cgattaatca aagaacaaca
     4861 gccgctgttt aataaacggt tgcgccgcaa tcgccagctc tgtgccctgc aattaaatga
     4921 aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt tcacgagccc ccaacctgtt
     4981 tggcctgttt gccaataggc gcgcagcttt gcaagcattg cagagcatcg ctgatgaaca
     5041 aaaactttgt tatggcctgt tgggactgga accgttaagt cgcggtcgtg catgttttcg
     5101 ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa gagagccatg aggaacatgc
     5161 gctacgcttg cgccagtctc tggagcgttt gcgagtggtg tgttggcctt ggcaaggtgc
     5221 ggtggcactg aaagaacagc acccggaaat gactcaatat catattattc aaaactggct
     5281 gtggctgggg gcggttaatt cgctggaaga agcgacaacg ttaattcgga cacccgccgg
     5341 gtttgatcac gacggttata aaattctttg taagccgctg ctttccggta actatgaaat
     5401 tactgaactt gatccggcga atgaccagcg agccagttga tttcactgaa caacagttta
     5461 ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga accagcacac gccctggtcg
     5521 gtagtgagca gtttgtcgcc cagttgccac gccccgttaa tcacaaacac caccccgccg
     5581 cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga ctttcgcctt acagacatcc
     5641 aggcgcgtca taatattgaa atccatcgac atctgccctg ccgtcagttt cgcttttacc
     5701 acctggtccg ccgtaaaggc aaaaggctgt aacggtttta aggtatggtt aaagcggtct
     5761 gcgctttcaa ggagcatctc gccgccttcc agcaacgtca ctatcctttc catgccggga
     5821 aacaaagaaa actccccatt cgccgcgatg gatgcaatgc tggcacgcca gtaaaaatca
     5881 cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac cggcagcgtt tcgccacaga
     5941 ttcaccgaca ttttacgcat atcaaagtat tccatactcg ctcccggccc gttcttttca
     6001 ttgttatcgt tcaggtaacc gtggatggtt atcggcaact cgggtgattt acgttagtgg
     6061 tgatcaggct ttttttacaa ctgtttgaga agagaaaaga aaaccgccga tcctgtccac
     6121 cgcattactg caaggtagtg gacaagaccg gcggtcttaa gttttttggc tgaaagatta
     6181 ttcagcagtt gcaggcattt taccttttgc cgctggacgt tctgtcagac gcttctcaaa
     6241 attagcatta aattgctttt tctgttccgg cgtcaggatg ttgtaaattt tgttctgggt
     6301 ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc atttttgcga tctgcgcttc
     6361 agcttttgct ttatcgaagg tatcgctggc aatgatgtca tgcattgcgc ggcgttcttc
     6421 cagcggcgga cgtttcatct ggtcacgctg gcctttcatg atttcgtgga tctgctgttt
     6481 ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc tgatgcggac cgaacttgcc
     6541 tttgtggtgc atcatcggct tcgcgtcagc cggtgctgcg gtagtggtgt ctgcggcatg
     6601 ggccaggtta gccgcgccaa gagccagggt agaggcaaca aacagtgcag ttaatttacg
     6661 catattctat atccttcctt tcagttattt attacggctt tcttaagtag cgtgccgtgt
     6721 tgacgagatt aactttactg actttagcgt caattaatca gagcaacggt aaaacaatga
     6781 aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa agaaaatttg atggagagtg
     6841 atgagagaat attacaacac gatgattttg cagagattat gaagaactat accggatgac
     6901 tggtgataaa taaagcaaat aaccaggatt aatctgtatt aatttataag aaagcaactt
     6961 aatacccgca gaatgatttc tgcgggtaag tattagctta ttttttcgag cattaatccc
     7021 gcgcgtaatc ccaacgctac caacggatta gggaataaca catactctac atcatgggtt
     7081 acggtaaaac gttcctctcc gtcctgcgcc agcaatgttc ctttctcaaa cggcataaaa
     7141 ttcagcgtgt cacttgccat atgcatttcg aaggacggcg agtggcgagt aatttgcgaa
     7201 accacccgat aacggagcgg cggtgttctc acgataccga cactctcacc agatagcagc
     7261 gcagcaattg cgctggcagt tactgcaaac tggcgaagat cgttttgccc aaagggcaac
     7321 gctttgccaa gttccaacgt acaggccagc gcgccaaaat gtctgacgct gaaatgggta
     7381 aacgtaccac caggctcctg atggaacacc agcgcctcca gccccgccgc acccagccac
     7441 gtcagaaatt tctcgtccca gggaatgtcg cgttgcggta atacaccgaa ctgcggatgc
     7501 aaggagccac gaattgcggt atgtagatca aggtgccaac gcacagattc tttgccctgg
     7561 tcataaaaat cttccaggca ctgttccagt tcgcgcgccc gacaggtttc tccgctttca
     7621 gcaaatagct gccaacgacc gccaaacatt cggttcatat cgctatggca ataacgtttc
     7681 ccttgcttca gcgcaggagg attcccgagg atcaccagca accgccaacg taacgggatc
     7741 tcgccgtgag atatcgcgcc aagcaacgcg tccagcattt ccacaggtgc cgtctcatta
     7801 ccgtggatcc ccgcagaaat caccagcgcg ccttgcggtg gcgttaatgg cgtcagttcc
     7861 agcacaccat cgcccagcca gcgccagcga acgccgttga tttctcgctc ggtgataacc
     7921 ggttttttac ccgttaaggt cagagcaaga aaattatcca ttgccccctc cctcgcgctg
     7981 gaacggataa accgaaccga gattcagtaa ttggctcaat acatccagtg cttcccgccc
     8041 ttcgcgcagc aattgcgggt cggccagatc ggcagcagta agacgatcgc ggtagtaacg
     8101 atccacccag tcattgagcg cattaaacaa ggtatcgttc atcatcaccg ccggattcac
     8161 cgcccggcgt tcttcttctg tcaataccac ccgcaaccgc aggcacgccg ggccgccgcc
     8221 attcgccatg ctttcacgta agtcaaagac ttttagttcg ctaatcgggt tgtcagcggc
     8281 aaggagttca ttgagataac cccatactcc ggcgtgttcc cgacactcct gaggcagcac
     8341 caacatcatg gaaccatcat cgcggctcaa cagttggctg ttaaacagat aggtagacac
     8401 cgcatccgac acggaaacct gagttgccgg aacttctatc gccataaaac cgttcacccg
     8461 cgtgccagta actgtgactg gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg
     8521 ttactcacgg caatcacgtc attatgaaaa acgctctggt cgataacgtc cgggttttgc
     8581 tgggcgaaaa tcacctgttg gggattcacc tgattaagcc ttgccaccgc ctcgctggct
     8641 tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat cattgccttc ttctcgcccg
     8701 tagacaaaaa gtagcatacc cggttcaccg taatgaccgc cgagacgatt gtggtttgcc
     8761 gccccctcat caccgagcaa cgctacctgc ggcaacgccg aatggacgct aaatttctct
     8821 tcgtccttaa aaatcgcttt taacagcgat tcagtgacgg gcgcttccag cgaacggtga
     8881 aatttattgt tcaggttggc aacggtgaga tgcactttgc catccagcgt atcggcagat
     8941 ggcgcgatcg ttgccgcatt ggctacccac attggcgaag cggaactgac gctggaaagc
     9001 cagtgcggtg cctggcgtgc aactttttcc agtacctgct catcgctacc actgaatccc
     9061 aactgacgca gcaccggaat aaacggacgc tcgtgcggcg gaatcacggc ctgggggaat
     9121 cccgcatcgg caagggcttt cattttcagt aagccctgtt ttgccgccag tcgcgggtta
     9181 gacacctgaa aacggtgacg ggtagaggct tcattaccaa acgacaggcc cgcgtaatga
     9241 tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg cgttcatcgc accacctcat
     9301 cggaaaaatc cagcccgggg ttaagcgtgg cgggcaatgt taacgagtcc gactccaggc
     9361 tcgccatagg ccatgcgcag taatctgcgg cataccaggc gctggggcga tggttaccgg
     9421 aagcaccaat gccgccgaat ggggcggtac tggcagcacc agtaagcggt ttgttccagt
     9481 taacaatccc cgcccgcgcc tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa
     9541 ccagaccgca aaagagtccg aagcgagtgt tgttcgccat ccgaatcgcc tcatcaaaag
     9601 tatcataacg ccagacgcgc aaaaacggcc cgaacacctc ttcatccggc acaccgccaa
     9661 cgcccgtcat ttcaatgatc cctggtgtca gcaacgatgt ccctgcttgt aataagcgcg
     9721 gcgcaagcag ggttcgtccg cccatcgctt ccagttgctg ccaggcagta accacctgct
     9781 gtgcggcctg ttcagaaatc agcccgccaa taaacggctg cggttcgtca tcccagttgc
     9841 ccggcgttaa tcgctggctg acggcaacca gactagcaag aaacgcatcg ccctgcgctc
     9901 cgcttttgag caataaacgg cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg
     9961 actgaatggt cagatggaca gccgcgtcga tatccgccac ctcatcgata attagcggat
    10021 tattaccgcc catctcaagg gcgagaattt tctccggctg accggagagc tggcgatgca
    10081 gctggtagcc ggtattggcg ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac
    10141 tcagcgcctg acccgtttca cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc
    10201 cagcctgctg ccataaacgc attaccgctt cgccactcca cggtgtcagt tcgctgggtt
    10261 taaagataat ggtgttacct gccagcaatg ccggaacgat atgtccgttc ggcaaatgac
    10321 cagggaaatt atacggccca aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg
    10381 ccgcaccgtc cggcatttca ctacgctgct cgccggtacg aacgtgatac gccttaattg
    10441 atatcgcgat tttattgatc atcgccgtca cttcggttgc cgcttcccag cgcggcttac
    10501 ccgtttctct ggcaataatc gcggttaatt cggctttatt gctttccagc aatccggcaa
    10561 agcgttcgac aacgacctga cgttcagcca atgagagccg cgcccagcgc ggaaacgccg
    10621 cacgggctgc ccgacaagcc tgttcgacct gagcggcatc ggcatcattg ccttgccata
    10681 acacctcgcc cgataccgga ttacgcttca cacgcgatgc gccctggccc gttatccagt
    10741 caccgttaat ccataaagtc atgctgtttt ctcctctgcg cacaggcgca ccagacgaac
    10801 gcgatcccct gcgtggcatt tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc
    10861 ggttgccgga tcggtacgcg ccagcaccac gcggaaatgg tgataatttt cattggcgac
    10921 caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct gccacttcca ccagccgact
    10981 tttacggatg gcgcgcacgc ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat
    11041 gtcgatatag ttgcggtaac gaaaaccttc tttctccagc accgcgcggg caggcgcggt
    11101 ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg gataaaaagt gggtatagat
    11161 cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc
    11221 ggcgcggcta aaatccatcg aaaagaagcg tttaccgagg ctttgccaga acggtgaata
    11281 gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg
    11341 aaaagccgcc ataaacataa agcgcgattt cgacagcaaa tagccgttgc cctctttgcg
    11401 ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag
    11461 aaacagcgtc ggcaatgcgt tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc
    11521 gacgcgatag ttgtaccagg gatcgttcag cccaaccgcc acctcaatag cgcaaatccc
    11581 cgccacggtg cctgtctcgc tatcttccag cacgaacaca tagccctgct cgcttttggg
    11641 cagttcacct tgccaggttt tgattgccct ttcgatacgc gccgaaagcg tggcttcatt
    11701 ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca agctgcatca gcgccgagac
    11761 atctgagcgc tcaacgggac ggatgaccat catgatgaac ctcggctaac aaagtgttcg
    11821 caagcagctg caaagcgatc cagtccggtc gtcacctctt cttcgctgac attgagcgca
    11881 ggcgcaaaac gcaccacgtt gccacccgca atcagtacca tcacgcctgc tttcgccgct
    11941 tccagagaga tctgtttcgc ttgcccggcg taatcggcat tcagtacaca gccaatcaac
    12001 aaacctaagc cgcgaatttc actgaacaaa ccatagcggt gattaatagt attaagacgc
    12061 tcaacaaacc agtcgtgacg ctgtttaacg ccattaagca tctctggtgt gttgatgagc
    12121 tccagcactt tgcctgctac cgccgaggcc agcggattac cgccataggt ggtgccatga
    12181 gtgccaacgg tcatcacgcg ggcgcactct tcggttgcca acaacgcacc gaccgggaaa
    12241 ccgccgccca gcgctttggc ggtggttaac agatcgggcg tcacgccgta gtgcatatag
    12301 gcatacagct caccagtgcg cccgacgccg gtttgtacct catcaaaaat cagcaaggca
    12361 ttgtggcggt cacacaattc acgcagacct tgtaaaaacg cgttgctggc tggcaccaca
    12421 ccgccttcgc cctagatggg ctcgacaatc accgcacagg tggcgtcgtc aatcagcgcg
    12481 ctggcagagt taatatcgtt atatgcagca tgacgaatat ccgccggcag tggcgcaaaa
    12541 tcctgtgaat aggctggctg cccacccgca ctgacagtaa acagcgtgcg accatgaaac
    12601 gcatttttga acgccacgat gccactctta tggctgccgt agcggtcgtg agcgaattta
    12661 cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg agttacaaaa gaagacgcga
    12721 tcggcaaacg tggcgtcgat caattttttc gccagtcgca gtaccggctc gttggtgtaa
    12781 ccgttgccgg tatgccagaa cttactcgcc tgttcgttca gcgcttcacg cagttccgga
    12841 tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga agtcgatata ctctttcccc
    12901 tgctgatccc acaagcgcga accttcgcca cgtaccggta taaagggtgc cggagcgtaa
    12961 acaggtatca tccattcatc aaagttttca cgcgtaattg gctgagacat agcgacctct
    13021 acagtaaata attcgttatt tgtatgttaa caataagtaa tgtttgcgct gtaaatgtag
    13081 attgcagggt tcgtgccagc cagtgataaa agtgcataaa cggcggaggc taactggaaa
    13141 tcaaggagtt ataaccaaac catatgcatt taaagtgcat ataaagtgaa tacgtttgcg
    13201 atgtgggtga ataaaaagaa taaaaaacgc aatgttatgc agaagtaaaa tataattcta
    13261 gaattgtgat cattgacgaa atttactgga aattactgcg ccattctgac gcagcgcgca
    13321 ccaaaagcgg gcattttttg cgccatcgtt gacatcatta acaaccatcg atcaaatcac
    13381 ttaacaacag gcggtaagca acgcgaaatt ctgctaccat ccacgcactc tttatctgaa
    13441 taaatggcag cgactatgaa atttgtctct tttaatatca acggcctgcg cgccagacct
    13501 caccagcttg aagccatcgt cgaaaagcac caaccggatg tgattggcct gcaggagaca
    13561 aaagttcatg acgatatgtt tccgctcgaa gaggtggcga agctcggcta caacgtgttt
    13621 tatcacgggc agaaaggcca ttatggcgtg gcgctgctga ccaaagagac gccgattgcc
    13681 gtgcgtcgcg gctttcccgg tgacgacgaa gaggcgcagc ggcggattat tatggcggaa
    13741 atcccctcac tgctgggtaa tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc
    13801 cgcgaccatc cgataaaatt cccggcaaaa gcgcagtttt atcagaatct gcaaaactac
    13861 ctggaaaccg aactcaaacg tgataatccg gtactgatta tgggcgatat gaatatcagc
    13921 cctacagatc tggatatcgg cattggcgaa gaaaaccgta agcgctggct gcgtaccggt
    13981 aaatgctctt tcctgccgga agagcgcgaa tggatggaca ggctgatgag ctggggggtg
    14041 gtcgatacct tccgccatgc taatccgcaa acagcagatc gtttctcatg gtttgattac
    14101 cgctcaaaag gttttgacga taaccgtggt ctgcgcatcg acctgctgct cgccagccaa
    14161 ccgctggcag aatgttgcgt agaaaccggc atcgactatg aaattcgcag catggaaaaa
    14221 ccgtccgatc acgcccccgt ctgggcgacc ttccgccgct aatttagcag ctctcctggc
    14281 tcaaactggg tcaggagaat taaccttgag aaaaatcaac aaactgtcag taatgatttg
    14341 ttgcctgccg tcctttgtta taccgtcact gcgtttttag ttgtctgacc acttctctat
    14401 tatcaagttt gatataggaa actccacgat gaacgctgag cgtaactttc tgtttgcctg
    14461 tcttattttt gcgctggtca tttacgctat ccacgctttc ggtttattcg atctgctcac
    14521 cgatttaccc cacttacaga cactcattcg ccagagcgga ttgttcggtt atagcctcta
    14581 tattctgtta ttcatcaatg cccccctctt tctgttacca ggaagcatac tggtgatcgc
    14641 tggaggaata gtttttggcc cgctcttagg gacacaactc tcattaattg ccgcaacgct
    14701 ggcctcctcg tgctcattcc tgctggcgcg ctggctggga cgtgatttac tgctgaaata
    14761 cgtgggccat agccatacct tccaggccat tgaaaaaggc attgcgcgta acggtattga
    14821 ttttcttatt ctgacccgct taatcccgtt gtttccttac aatattcaaa attacgctta
    14881 cggattcacc accatcgcct tctggcctta tacccttatt tcggcactca cgaccctgcc
    14941 cggtattgtt atttataccg tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg
    15001 ctttatttta caactctgtc tggcgggcct ggcgctgttt attctcatcc agctcgcaaa
    15061 actctacgcc cgacacaaac atgtggatct gtctgcttcg cgccgcagcc cacttactca
    15121 cccaaaaaat gaaggataga acgatgtcgc aacattattc agtgtcatgg aaaaaaggac
    15181 tggctgcact ctgcttactg gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg
    15241 cggcggcaaa agtggaatac gacggacttt cgaacagcca accgctgcgc gtcgatgcca
    15301 ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg tttcctcacc gacgacactc
    15361 gtcacggtat tgtgtttaaa gatggctcca acggacataa atcgctgttt atggcttatg
    15421 cgcccccgaa agcattttat gaagccctga aagaggcagg tggtacgccg ggcgaaaaca
    15481 tgacgatgga taataaagaa acgactcatg tcacaggcag caaactggat atttcggtca
    15541 actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt gattgttgac agtaatggca
    15601 agaaactgga catgcgcttt ggcggtaatt taacggcagc agaagagaag aaaacgggtt
    15661 gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag caatgcaaca tacacttatg
    15721 gtgcggtgga aaaacacggc gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg
    15781 ataacacgct ggcaacggtt acctttaaaa tcaccgaata aagccaagat aaaggatgat
    15841 gaagatacaa tcgcgtaaaa tctggtacta ccgtataacc ctcatcatcc tgttgttcgc
    15901 tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc
    15961 ggcgtttgcc gccgtggacc aacagggtat agaacgcttt attcagtctt acggcgcact
    16021 ggcggcagtt gtctcgttct tgttgatgat tttacaggcc attgctgcac cgctacctgc
    16081 gtttttgatc acctttgcca atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc
    16141 gtggaccagt tcgatggccg gcgcggcgct gtgctttttt atcgccagag tgatgggccg
    16201 cgaagtggtg gaaaaattaa ccggcaaaac cgtgcttgac agtatggacg gctttttcac
    16261 tcgctacggc aaacacacca tcctggtctg tcggttattg ccttttgtcc ctttcgatcc
    16321 aatcagctat gctgccggtc tgacttcaat acgttttcgc tcgtttttta tcgccaccgg
    16381 gcttggtcaa ttaccggcga ctattgttta ttcctgggca ggcagcatgt taacaggcgg
    16441 tactttctgg tttgtcaccg aactgtttat tctgtttgcc ctgaccgtgg tgatttttat
    16501 ggcgaagaaa atatggcttg agcgccagaa gaggaatgac tgatgggttt accgccgctt
    16561 agcaaaattc ctttcatttt acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag
    16621 gtgctaagcc cgattcgctg gtgggggcgg atcccgttta tcttttattt ggtgtcaatg
    16681 tttgttgggt ggctggagcg caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc
    16741 agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgcg tggatatcac cagtatgaaa
    16801 gtcgccgagc gtaccggcag caccgataaa ctgctggcag tggctgactg gcggcaaagc
    16861 ccgctcttta gcgatgaaga acggctggcg ctggagtacg ccgaagccgc aagcgtcaca
    16921 ccgccaacag tcgatgatgc cctgcgtacc cgactggctg cgcattttga cgctcaggcg
    16981 ctcaccgaac tgacggcatt gatcggcctg caaaacctgt cggc
//
LOCUS       DAJTYM010000084        16832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTYM010000084 DAJTYM010000000
VERSION     DAJTYM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..219)
                     /locus_tag="OO575_002903"
     CDS             complement(<1..219)
                     /locus_tag="OO575_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Specifically methylates the ribose of guanosine
                     2251 in 23S rRNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6665792.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIAR"
     gene            complement(399..2840)
                     /gene="rnr"
                     /locus_tag="OO575_002904"
     CDS             complement(399..2840)
                     /gene="rnr"
                     /locus_tag="OO575_002904"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6665793.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2879..3304)
                     /gene="nsrR"
                     /locus_tag="OO575_002905"
     CDS             complement(2879..3304)
                     /gene="nsrR"
                     /locus_tag="OO575_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6665794.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3509..4807)
                     /gene="purA"
                     /locus_tag="OO575_002906"
     CDS             complement(3509..4807)
                     /gene="purA"
                     /locus_tag="OO575_002906"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6665795.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4911..5108)
                     /locus_tag="OO575_002907"
     CDS             complement(4911..5108)
                     /locus_tag="OO575_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6665796.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5190..6194)
                     /gene="hflC"
                     /locus_tag="OO575_002908"
     CDS             complement(5190..6194)
                     /gene="hflC"
                     /locus_tag="OO575_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6665797.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6197..7456)
                     /gene="hflK"
                     /locus_tag="OO575_002909"
     CDS             complement(6197..7456)
                     /gene="hflK"
                     /locus_tag="OO575_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6665798.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7542..8822)
                     /gene="hflX"
                     /locus_tag="OO575_002910"
     CDS             complement(7542..8822)
                     /gene="hflX"
                     /locus_tag="OO575_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6665799.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8898..9206)
                     /gene="hfq"
                     /locus_tag="OO575_002911"
     CDS             complement(8898..9206)
                     /gene="hfq"
                     /locus_tag="OO575_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6665800.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9292..10242)
                     /gene="miaA"
                     /locus_tag="OO575_002912"
     CDS             complement(9292..10242)
                     /gene="miaA"
                     /locus_tag="OO575_002912"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6665801.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10235..12082)
                     /gene="mutL"
                     /locus_tag="OO575_002913"
     CDS             complement(10235..12082)
                     /gene="mutL"
                     /locus_tag="OO575_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6665802.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12092..13429)
                     /gene="amiB"
                     /locus_tag="OO575_002914"
     CDS             complement(12092..13429)
                     /gene="amiB"
                     /locus_tag="OO575_002914"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6665803.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13448..13909)
                     /gene="tsaE"
                     /locus_tag="OO575_002915"
     CDS             complement(13448..13909)
                     /gene="tsaE"
                     /locus_tag="OO575_002915"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6665804.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13881..15428)
                     /gene="nnr"
                     /locus_tag="OO575_002916"
     CDS             complement(13881..15428)
                     /gene="nnr"
                     /locus_tag="OO575_002916"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6665805.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15427..16566
                     /gene="queG"
                     /locus_tag="OO575_002917"
     CDS             15427..16566
                     /gene="queG"
                     /locus_tag="OO575_002917"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6665806.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16549..16602)
                     /locus_tag="OO575_002918"
     CDS             complement(16549..16602)
                     /locus_tag="OO575_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665807.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3821 a         4793 c         4224 g         3994 t
ORIGIN      
        1 gcgggcgata atgccctgat gcacggcacc gtcgcttttc tcgtcgagat attggcggtt
       61 tgccaactgg ataaccacgc cctgagactc aagggcgtga atcagcggta acagacgttt
      121 atcttcacgg ccttttaaaa taaagacttc ctgaaaacgt tcaggggcgc gctccagcag
      181 ggcctgcact gcgtggatgc cgtaaatcat ttcgctcatt aatgtactcg ttgtttacgt
      241 tttgcctgat gcactacgtt tatccggcct acatgatctc tgcaatatat tgaatttgcg
      301 tgattatgta ggccggataa ggcgttcacg ccgcatccgg catgaacaaa gcgcattttg
      361 tcagcaatct aaccctcttc ttttaaagag ggtattgatc actctgccac ttttttcttc
      421 gccgcacgct tcgctttggt cgctgcggct attttctgcg ttttcgccga tggctttttc
      481 gcttttctcg cgtctttctt cgccgctttc ggcttcgttt ttttctcacc gcggaaggcg
      541 ctgtctggct caaagtttac ctttttaccg acctgacaac gcttgccgcc ttttttgcct
      601 gcatcgcctt ttttcgcttt ctcgcgcgcc gttttaccga cgttgcgcgg tgcgcgttca
      661 ctggagatca gactaaagtc gattttgcgc tcgtccatat taaccgcttc gacgcgaact
      721 tccacgcgat cgcccaggcg ataagtctgg ccgctggatt cccccatcag gcgttgccct
      781 acctggtcaa agcgatagta gtcattgtcc agcgaagaga catggaccag accatcaatg
      841 aacaagtcgt ccagacggac gaagaagcca aagccagtga cgctggaaat tacgccttta
      901 aagacgttac ctacctggtc gagcatgaag tcacacttca gccagtcagc gacatcgcgc
      961 gttgcttcgt cggcacgacg ttccgccatc gaacagtgct gacccagttg caacatctct
     1021 tccatcgaat aatggtagcc gccggtttca gtggtgttgc cctgatgccc ctgctctttc
     1081 gccagcagat atttaatggc gcggtgcagc gtcaggtcag gataacgacg aatcggcgaa
     1141 gtaaagtgcg cataggactg caatgccaga ccgaagtgac cacggttttc tggatcgtaa
     1201 atcgcctgtt tcatcgagcg tagcagcatg gtttgcagca tttctgcatc aggacggtcg
     1261 gcaaccgact ccagcagttc cgcgtagtca cgcggttccg gcttgttacc acccggcagc
     1321 tccagcccca gctccgccag cactgaacgg aaagaggtaa tcgcttcggt gctcggcttg
     1381 tcgtgaatac ggaacagtgc cggttctttc gctttctcaa cgaaacgcgc cgccgagata
     1441 ttcgccagaa tcatgcactc ttcaattaat ttgtgcgcgt cgttacgctg ggtctgttcg
     1501 atacgttcaa tacggcgttc agcgttgaaa atgaacttcg cttcttcgct ctcaaatgag
     1561 atcccaccgc gttcttcacg ggctttatcc agcactttat agaggttatg caactcttcg
     1621 agatgcttaa ccagcggggc gtactgctcg cgcagatcct gatcgccctg cagaatatgc
     1681 cagactttgg tgtaggtcag acgcgcgtga gagctcatca ccgcttcgta gaatttgtag
     1741 cccgtcaggc ggcctttcga cgaaaccgtc atctcgcaca ccatacacag gcggtctacc
     1801 tgcgggttga gcgaacacag gccgttagag agcacttccg gcagcatcgg gataacctgc
     1861 gaagggaagt acaccgacgt gccacggtta cgcgcttctc tgtccagcgg cgttggcgga
     1921 cgcacatagt agctgacgtc ggcaatcgcg acccataaac gccagccgcc gccgcgtttt
     1981 ttctcgcagt aaactgcatc gtcaaagtca cgggcgtctt cgccatcaat ggtgaccagc
     2041 ggtaaatcgc gcaaatcgac acggcccgct tttgcttctt ccggcacttc ttctttcagc
     2101 cccgcaacct gttgctcaac agcctgcggc cagatatacg gaatttcatg ggtacgcaga
     2161 gcgatatcaa ccgccatgcc ggtgcccata ttgtcgccca gcacttcgac gattttaccc
     2221 accgctttgg tgcggcgagt cggacgctga gtcagttcga ctacgaccac aaagcccatc
     2281 cgcgcgccca tgatctgatc gggcgggatt aagatatcga agctcagacg gctatcgtca
     2341 ggaaccacaa agccgacgcc cgcttcggta aagtagcgac caacaatctg gctggttttt
     2401 ggcaccagta cgcggacaat acgcgcttca cgacgacctt tacggtcagc acccagcggc
     2461 tgcgccagca cctgatcgcc atgaatgcag gttttcatct gctcgctgga gagatacaaa
     2521 tcatctttac gcccttcaac ccgcagaaag ccgtagccat cacggtgacc aataacggta
     2581 cctttcacca ggtcgaggcg ttccggcagc gcatagcact gacgacgagt gaagaccagt
     2641 tgaccatcgc gctccatcgc gcgcaggcgg cgacgcaggc cttcaagctg ctcttcgcct
     2701 tcaatgtgca gttctaccgc cagctcatca cggctggccg gtttttcacg tttggttaaa
     2761 tgttcgagga taaattcccg actagggatg ggattcgcgt atttttcagc ttcgcgttcc
     2821 tggaaaggat cttgtgacat ctcggttcct ccgttgtcat ctctgatgaa gattttcgtc
     2881 actccaccag caataattta taaagcggtt gattctcttc aaccaaatcg gcaagcgtgt
     2941 agttatccag ttccgtaaga aaactttgca cggccttaga aagcgcctgt ttcaaccgac
     3001 aggcaggtgt aatgtggcaa aactcactgc tgcaattcac cagcgataag ggctccagct
     3061 cgcgcaccac atcaccaata cgtatcgcac tcgccggttt acccaggcga atgccgccat
     3121 tttttccacg aacagcagtc acgtagccgg cacgactaag ttgattgatt attttgacca
     3181 tatgattacg ggagacgccg tagacgtcag tcacttcaga aatactggtc atccgccctt
     3241 ctggcaatga cgccatgtag atcagcgcac gtaatccgta atcagtgaaa ctcgttaact
     3301 gcacatcaac ctcaaaaggg aaatcgggaa aaaatacatt tatattgatg ataaaccagc
     3361 cacaagctgt gtcgctaatt tatttcagtt cggggaagga aaaaagcgag gatttaacac
     3421 tgtgtcggat agcgtaatgg cttaaccgac acagcaatgt atcacactgt tagcccggca
     3481 ggcaaaatat ctgccaggcg taccagaatt acgcgtcgaa cgggtcgcgc agaatcatgg
     3541 tttcagtacg atccggaccg gtagagatga tatcgatcgg cacaccggtc agctcttcaa
     3601 tacgcttgat gtagttcagc gccgcctgcg gcaggccgct acgatctttc acgccgaaag
     3661 tggattcaga ccagcccggc atggtttcgt aaatcggctc tacacctttc cagtcgtcag
     3721 ctgccagcgg agtggtagtc acttcgcgac catccggcat acggtaagcc acgcagagtt
     3781 taacctcttt caggccatcc agaacgtcca gtttagtcag gcagaagcca gacagggagt
     3841 tcagctgtac cgcacgacga acggcaacgg tgtccagcca gccggtacga cgacgacgac
     3901 ccgtagttgc accgaattcg ttaccctgct tgcagaggaa ctcgccagtt tcatcaaaca
     3961 gttcggccgg gaaaggacct gcacctacac gagttgagta agctttgagg atacccagaa
     4021 cgtaatcaac ataacgcggg cccaggccgg aaccggtcgc cacgccacca gcagtggtgt
     4081 tggaagaagt tacgtacgga taagtaccgt ggtcgatatc cagcagcgta ccctgcgcac
     4141 cttcgaacat gacgaaatcg ccacgctgac gcgcctggtc gagcaggtca gaaacgtcaa
     4201 ccaccataga agtcaggatg tcggcaacag ccatcgtatc atccagaact ttctggtaat
     4261 caaccgcttc agctttgtag tagttaacca actggaagtt gtgatattcc atcacttctt
     4321 tcagtttttc agcgaaggtt tctttgtcga aaaggtcgcc aacacgcaga ccgcgacgtg
     4381 ccactttatc ttcataagca ggcccgatac cacgaccggt ggtgccgatc gctttcgcgc
     4441 cacgcgcttt ctcacgcgcg ttatccagag caacgtgata atcaaggatc agcggacatg
     4501 cttcagacag cagcagacgc tcacgaacgg ggatgccacg gtcttccagt tctttcatct
     4561 ctttcatcag cgcggccgga gacagcacaa caccgttacc gatgatgctg gttacattct
     4621 cgcggagaat acctgatgga ataagatgga gaacggtttt ttcaccgttg attacgagag
     4681 tatggcctgc gttgtgaccg ccctggtagc gtacaacata tttagcccgt tcagtcagaa
     4741 gatcgacgat cttaccttta ccttcgtcac cccattgggt gcccagtacg acgacgttgt
     4801 tacccatttt tcaaaatcac cgtttgctta aaaatggatt ctaccatcgc tttttcagag
     4861 ttacagcact ttttgcactc aaaaatgacc tcgatcagtc tgttttttgt tcagccaatc
     4921 gttttcctca acatgtagta gaccacaacg cccgcaacca caagtccacc gccaaaacga
     4981 cgtaaaatat tatcgggcaa attggtcatc gcagagatca tcttcttcca tgccttcggg
     5041 taaagcatcg gccctaaacc ttccagtacc aaaaccaggg caagcgccag ccagattgtc
     5101 gaattcattc attatcctta tagaaaaaga aaaccaccga catccctgag gatgcggtgg
     5161 ctttattgac ctgtaccgca gtcgttatat taacgcgttg cggaagtcgg cgtcttcatg
     5221 tagcggaaga aatcgctatc cgggctcatg accatcacgt cctgattgcc agagaagctg
     5281 ttctcataag cacgcaggct acggatgaat gcgtagaagt ccggatcttt actgaatgca
     5341 tcagcaaaca gtttggctgc ttcggcatcg ccttcaccac gcataatgcg gccctgacgc
     5401 tcagcttctg ccagcgttct ggtcacttca tagtcggcag tcgcgcgcag tttttccgct
     5461 tcttcctgac cttgtgaacg gtgacgacgc gctaccgctt cacgctcggc gcgcatacgg
     5521 ttgtagatcg cttcagacac ttcggtcggc aggttgatct gcttgatacg cacatcgaca
     5581 acttcaatac ccagcgccgc catactgttc gggttgatga ccggaacttt gcccttcgtc
     5641 tcagccgtta cgcgctctgc cgcttcggca atggcgttat ctgctgccgg ggtagtaact
     5701 tcatcttctg tacccgcaga accggagttc agcgcgtcac gtacttcgag ggtcagacga
     5761 ccacgggaat cggtgacgat atctttcacg tccaggcgac caatttcaga acgcagacgg
     5821 tcagagaact tacgtttcaa cagcacttcc gcttgcgaaa tgtcgccacc acccgttgcc
     5881 aggtagtaac ggctgaaatc gctgatgcgc catttgatgt aagagtcgac gatcaggtct
     5941 ttcttctctt tggtcacaaa gcggtcggcc tggttgtcca tggtctgaat acgtgcgtcg
     6001 agcattttca ccgtttcaat gaacggtatc ttgaaatgca gaccaggctc ataaaccaaa
     6061 ggtttgttgt catcgtcacg cagtacctta ccaaaacgca gcgtaatacc gcgctcacct
     6121 tctttgacga caaagacaga catgtaaagc actaccagca cgatgatgat aatcgcgata
     6181 actgacttac gcatcgttat tccccctgac gctggtagtc gttacgctgc gcgttggcgc
     6241 ggcgttggtc cataatatcg ccctgactgg tggacgacgt gttgcttgct ccactggttg
     6301 tggaggaaga ggctggcggc agacgcagca gattgctggc accgttatcg ctcttcgccg
     6361 caggggcgtt accacctttc agcatctggt ctaacggcag aaccatcagg ttgccacctt
     6421 tatcgttaac cagcactttg cgggtgttac ccaacacttt ttccatcgtc tcgatataca
     6481 gacgctcgcg agtaatttcc ggcgcggctt tatattccgg cagaagttta gcaaagcgcg
     6541 ccacttcacc ctgagcttcc aggatggtct gggccttgta cgcacgcgcc tcttcgagga
     6601 tacgttgcgc ctgaccgttc gcacgcggct gaacttcgtt ggaatacgct tctgcttcac
     6661 gaatgtattg ctgttcgttt tcacgcgcgg caatcgcatc gtcaaacgcc gcttttactt
     6721 cttccggcgg acgagcagcc tggaagttga cgtccagcag cgtgataccc atgtcatacg
     6781 gacgaatcgt ctcttccagt tcgcgctgag tatcgctacg aatcacggta cgaccttccg
     6841 tcagaatgcg gtccatggtg tatttaccga taactccacg cagggcgctg tcggtagcct
     6901 gacgcaggct gtcatccggg ctggtcacgc tatacagata tttttccgga ttggtgacgc
     6961 ggtactgcac gttcatctca acgcgcacta cgttctcgtc cgacgtcagc atcacaccag
     7021 aagcggccag ttcacgcacg gcttccacgt tcaccggttt gacttcgtcg ataaacgtcg
     7081 gtttccagtt cagacccggc tcaaccagat ggctgaattt accaaagcgt gttaccacgc
     7141 cgcgttcggc ttctttaatg gtatagaaac cactggccgc ccagataatg acaatcgctg
     7201 ccgctgcgat ggtaacgaca cgaccgccaa gctgcgggcg cgggccttgc gatgaactgc
     7261 caccgccaga tccggtgcct ttaccgccgc ccagaccacc gagctttttg ctcagtttgc
     7321 ggaagatatc atctaaatca ggtggccctt gatcgcgacc gcctttgttt ccatttccct
     7381 cagagttgcc gccaggtttg ctgcttcccc acgggtcgcg gtcttgtccg ttattaccgg
     7441 gctgattcca cgccatgttt gtgctccata tttgttatgc ggtgatccct gttgtctttc
     7501 gacgccagag gatatgactc cacgcttcag acgctacgcc gttagatcag gtaatcgatc
     7561 aacgccggtt cttgtttaca gaggcgacgc cagtcaacga tcggcatacg aacttgcaga
     7621 cttacgctgc cgtcctcctc catccactct ttttctattg cctgaagctg ataaaaacga
     7681 ctcctcagac gcccttcctg cggtggcaga cgcaatgtat gctgcgccac ctcgccggaa
     7741 agccgctccg tcaaagcctg aaaaagctgt ggtatccccg ctccggtctg tgcggaaagc
     7801 cagacacgga tcggtttgtt ctcttcgtcc cgatcaatac gcggttcgaa atcttccagc
     7861 atatcgatct tgttcatcac cagcagggtt gggatctcgt gagcgtcgat ctcttcaaga
     7921 accgtattca ccgcttcgat gttttcttgt acacgcacat ccgccgcatc aatgacgtgc
     7981 agcagtaatg tggcttgccg cgtctcttgt aacgtggctt taaatgccgc caccagatcg
     8041 tgcggcaggt ggcgaataaa ccctacggta tctgcaagta cggtttcacc gacatctgca
     8101 acgtcaatac gccgcaacgt cgggtcgagg gtggcaaaca actggtctgc cgcgtagacc
     8161 cgcgcttcgg tgatgcgatt gaaaagggta gatttaccgg cgttggtata tcccaccagc
     8221 gaaacagtag gaacgtcggc tttgatacgc gattgccgcc cctgctcacg ctgcttttca
     8281 actctttcca ggcgcgactg tatctgcacg atgcgattac gcaacaaacg acggtcggtt
     8341 tcgagctggg tttcacccgg accacgcaaa cctatcccgc ctttctgtct ttcaaggtgg
     8401 gtccagccac gcaccaggcg cgtagccaga tggcgcagct gcgccagctc aacctgcaac
     8461 ttaccctcat gggtacgcgc acgttgggcg aaaatatcta aaataaggcc ggtgcggtcg
     8521 ataacacgac actcgcacaa acgctccagg ttacgctctt gcgccgggct cagggcatgg
     8581 tcaaaaagaa cgaccgaagc acccatcgct ttgacagctt ccgcaatttc aactgcttta
     8641 ccttcaccta caaaatactt tgggtgcggc gctttacggc taccggtaat cacctgcaat
     8701 gcttcgacac cggcggaaga gaccagagat tcaaactcct ggaggtcttc catatctttg
     8761 tcttgcgtaa aatagatgtg taccagtacc gcctgctcac cagcatcata acggtcaaac
     8821 aagcgtataa ccctctaaat agatcagcgg ggaacgcagg atcgctggct ccccgtgtaa
     8881 aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga agtattctgc
     8941 gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt actgtgatga
     9001 gaaaccgggc gagatgggac aacagtagaa atcgcgtgct tgtaaaccat ctggctgacc
     9061 gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg cagcttaata
     9121 ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt caggaacgga
     9181 tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat ttgtactttg
     9241 aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca ttcagcctgc
     9301 gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt cactgtcaag
     9361 ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca actgtctcgt
     9421 ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa ggtaagacca
     9481 catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat ctcctcgggc
     9541 aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa aacgctgctc
     9601 aatgcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat gcacctgata
     9661 cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa
     9721 aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg ctgccgcaac
     9781 cggatctacc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg
     9841 ctcaattctg gcccgcactt ccgggtctgc cgacggtagc ggcgacaacc cttccagcaa
     9901 tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg ccgcggtgat
     9961 atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag cctgcgacgg
    10021 atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc
    10081 cgtcccgata tccatccctt tgtaaataag gacagaatca acgcttatca actctactgg
    10141 taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg gccccatcaa
    10201 aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg gcttttatcg
    10261 ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca agttgcggac
    10321 ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac tgcgcatgtt
    10381 cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat acggactgct
    10441 tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg cgtaagggta
    10501 aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata cccaattccg
    10561 ctagggcaga ctgcgctttt tctaatgccg atttttcttc gccagaaact tttagccgca
    10621 acggaatcag cagcggctgg gcgcaaacgg gcgcttcacc cggcgtcaat tgtgcctgac
    10681 gcagccaacg ttctgccact ggcaaggata aaagtgaaat gttgccatcg cgctccagca
    10741 acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg ttcgccgcaa
    10801 gtgcaggttc ctgcggttcc ggtgcttttg gtttttgcat cggcgcgggc gtttgcaaaa
    10861 gctggcgata cacttcacct tgctgtttct ggtagcctgg ctgcgcattc ggccaggggg
    10921 cagccggacg actgcctgag gcgggcgcag gagagtagcg cggagctacc ggctcacgag
    10981 ctgccggttc tgcaaagtga ttgcgccccg ccgccacgcg gttttccggg atggcacgcg
    11041 gtgcgggttg tggttcatcg tccagcggta gcggcgtttc cagttgctgt tgtagcacgc
    11101 tcagcacgcc ctgatagata aaatcatgca ccagacgcga ctgatggaaa cgcacttcgt
    11161 gtttggcggg gtgtacgttg acgtccacct gatgcgggtc gatctccaga tacaacacaa
    11221 atgccggttg ctgatcggcc cccagtttgt cttcgcaggc ctggcggatc gcatgattga
    11281 tcaggcgatc gcgcatcatg cgaccattca cgtagcaata ctgaatttcc gccagtgcgg
    11341 gcgtagtgtg atttggatcg gccacccagc cgcgcagggt gagatcgccg tgttgccatt
    11401 caatcgccag cgcttgttcg agaaaagcgg tgccgcaaat cgcgcctaag cgccgttctt
    11461 tttgcccgcc ttccggcact gcgcggtact gacgcacaat tttaccgtta tgcgacaggt
    11521 tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcggat gatttcatca atatggctaa
    11581 attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag aacagatcca
    11641 gcacctccag cgtcgttccc accggatgcg ctgccggttt aaccgtcacg tccatgtcgc
    11701 gtccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa gtgagcgtca
    11761 ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg ctgataatgg
    11821 cttcgagatc gtccagagag gcgattttac tggtggcatg acgagccagc gccagcgcca
    11881 gctcatcttt tttgataccg cagccgttat cacgaatgcg gataagtttc gccccaccgc
    11941 gttcgatatc aatatcgata cgcgtcgcac ctgcatcgag gctgttttcc actagttctt
    12001 tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc gccagttgtg
    12061 gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga tctggcgtcg
    12121 tcaccgtact ggcagtttgt gccgttgcac cctgcggcgc agattgcatc ggatgcgcaa
    12181 ggaagtaatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg taatcgtcgc
    12241 tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc agtactgatg
    12301 ggatatccgg cgagcgcaga acgccaaggc tggcgtgttc tggtcgacgt ttatgtattt
    12361 cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct acccgctggg
    12421 aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcagac tgactgttcg
    12481 ccagcacatc acccgcccca cccagcagct ccgacagttt ctcgtgctgc tccagccagc
    12541 tggccatttc actgttggcg cgacggttag agagcaccca tacggaagcg ccagtcgcac
    12601 tacggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt tgcttatgtg
    12661 ccacatcact gcgccccatc accgagataa agtaatcccc gtcacgggtt aaaacgcctt
    12721 taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg atggtgacat
    12781 ttttctcccg cgtaccaccg gggccgatag cgccagggtc ctgaccgccg tgtccggcat
    12841 caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc ggacgcgtta
    12901 ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac ggattgcgcg
    12961 ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc gcaaccacgg
    13021 gcggcggagg aggcggtggc ggcacatcgg cgttaatcgt aaagacgaca gtgtaattgc
    13081 tgccattctg ccgcttcacc gcttcggttt taccgttttc ggtaagatcg accaccagcc
    13141 gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc cttcaccaga ttattgccgc
    13201 tgaacaacaa cggcagtccc tgaatcatgc ccgtttgttt gatatcgagc gccacggtgc
    13261 gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac gttatccgcg
    13321 cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc accggcgtgc
    13381 acagcagcag cagcgtcgct accaaccaat ttctgatgcg atacatcatc ccgccacctt
    13441 tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa accgcactca
    13501 cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc gggtcaggaa
    13561 gaacacctgt accttgttgt ggccactcca ccaggcagat ggcatcgttg gcaaaataat
    13621 cgcggatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat
    13681 agaccattaa gttgtcgagc gtatagggtt cgaccagcgt ataagtgggg cttttgacat
    13741 taccctgatg acccagagcc tgtaaaaagc cccggctaaa ggtggtttta cctgcgccta
    13801 aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc cgctcgccca
    13861 ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg gtttttatca
    13921 gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc cagcatcccg
    13981 cgcgttccaa aacgcgccgc cagtacgtca gctgccgcac cgtgcgcgac acagcctgca
    14041 caggctgcat catacggcga cagtttttgc ccaagcaatg cgccaataat accagagagc
    14101 acatcgccca tgccgccgct cgccatgcct gcatttccgg catcaataat gcctaaagcg
    14161 tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc gccataacgt
    14221 tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac ggaacagcct
    14281 aacaaccgtg cggcctcgct aggatgcggc gtgatcacgc gattgtgacg cttatcggga
    14341 ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg aaaattctca
    14401 actttttgca gggctttttt cccccactct tgctggccca gaccgggacc aatcaccacc
    14461 acatcggccc attccaggct ttcggtaaga gagtccatag tcagttcatg caccatcaat
    14521 tctggtcgtg cagtcagcag cggcgcaatg ttctcactgc gggtcagtac tcggactaaa
    14581 ccagcaccag cacgtagcgc cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga
    14641 tcgccaccga taatcaccag ccgcccgtga tcgcctttat gcgaagtcgg gcgacgcggt
    14701 ttcagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc ctgacctgcc
    14761 agccagctat ccagccccag tgagtcaaaa tgcagttgtc ctgtaacatc ccgcgctttt
    14821 ccagtgagca agcctagttt cagcgcaata aaagtgatgg tgtgatcggc gttgatcact
    14881 gcgcctggcg tagcgccggt ttcagccagc aggccggaag ggatatcaac cgccacaatc
    14941 ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg gggcgcttgc
    15001 tgcaagccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg ccagacaata
    15061 ttcgaagcat ggatctcgcc acccgcgttt aaccatgctt cgcgtgccag cgcggcctct
    15121 tccggcaacg gtttgtcgct ctcctgggcc aacaacgtga cctcaatgcc gaccgctttg
    15181 gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca cagcaccagc
    15241 cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc gccagcgcga
    15301 agcatcagct catagagtgt gagccccagc acatctgccg cctcgcgttc tccgcggcgg
    15361 atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg ggtttttctt cattgtatgg
    15421 tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa acagtggggg
    15481 ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga gtccgagccc
    15541 aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat ggcacgtcac
    15601 ggtatgctgc gcgctcgccc ccatgagtta ttacccggta cgctgcgtgt aatcagcgtg
    15661 cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa ccccaaactc
    15721 ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg caaccgactc
    15781 aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag accgtttgtc
    15841 gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg ctggacaggt
    15901 aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct tctttttagg cgaattgctg
    15961 gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggcaa atgcgtggcc
    16021 tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga tgctcgccgc
    16081 tgtatctctt atctcaccat cgaacttgaa ggggcgctcc cggaagagtt gcgaccgtta
    16141 atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg gaatcgctat
    16201 tcgcaactca ctacagaaga cgatttcagc ccgcgtaagc cgctacacgc accggaactc
    16261 attgagttat ttgcctggag cgaagagaag tttttaaaag tcacggaagg atcggcgatt
    16321 cgccgtatag gtcacctgcg ctggctgcgt aatatcgccg tggcattagg caatgcccct
    16381 tgggatgaaa cgattttgac agcgctggaa agtcgtaaag gtgagcaccc acttctcgat
    16441 gagcacatag cgtgggcgat tgcgcagcaa atagagagac gaaatgcgtg catagtcgaa
    16501 gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg aaaaagggtt accgcgtgac
    16561 gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc aagacgaata
    16621 aaatcgtcaa gtgatcgact cgacaaaacg gaatcttgat ttttaactta tttatcaata
    16681 ggttaaaaaa ataccattca ccgtgcgaag tttctttgaa attaagatta cagcctgagg
    16741 ctgtggataa ctctgtttac aagagtttgt cagagacaac aaaaaccagc aataacgcat
    16801 cgcgtcgctg tagatatttt attgagagaa ga
//
LOCUS       DAJTYM010000085        16535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTYM010000085 DAJTYM010000000
VERSION     DAJTYM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            127..1260
                     /gene="fepE"
                     /locus_tag="OO575_002919"
                     /pseudo
     CDS             127..1260
                     /gene="fepE"
                     /locus_tag="OO575_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
     gene            complement(1257..2072)
                     /gene="fepC"
                     /locus_tag="OO575_002920"
     CDS             complement(1257..2072)
                     /gene="fepC"
                     /locus_tag="OO575_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6665808.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(2069..3061)
                     /gene="fepG"
                     /locus_tag="OO575_002921"
     CDS             complement(2069..3061)
                     /gene="fepG"
                     /locus_tag="OO575_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6665809.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3058..4062)
                     /gene="fepD"
                     /locus_tag="OO575_002922"
     CDS             complement(3058..4062)
                     /gene="fepD"
                     /locus_tag="OO575_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6665810.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4173..5405
                     /gene="entS"
                     /locus_tag="OO575_002923"
     CDS             4173..5405
                     /gene="entS"
                     /locus_tag="OO575_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6665811.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            complement(5409..6365)
                     /gene="fepB"
                     /locus_tag="OO575_002924"
     CDS             complement(5409..6365)
                     /gene="fepB"
                     /locus_tag="OO575_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6665812.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            6657..7832
                     /gene="entC"
                     /locus_tag="OO575_002925"
                     /pseudo
     CDS             6657..7832
                     /gene="entC"
                     /locus_tag="OO575_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7842..9452
                     /gene="entE"
                     /locus_tag="OO575_002926"
     CDS             7842..9452
                     /gene="entE"
                     /locus_tag="OO575_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6665813.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            9466..10323
                     /gene="entB"
                     /locus_tag="OO575_002927"
     CDS             9466..10323
                     /gene="entB"
                     /locus_tag="OO575_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6665814.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            10323..11069
                     /gene="entA"
                     /locus_tag="OO575_002928"
     CDS             10323..11069
                     /gene="entA"
                     /locus_tag="OO575_002928"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6665815.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            11072..11485
                     /gene="entH"
                     /locus_tag="OO575_002929"
     CDS             11072..11485
                     /gene="entH"
                     /locus_tag="OO575_002929"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6665816.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            11666..13771
                     /gene="cstA"
                     /locus_tag="OO575_002930"
     CDS             11666..13771
                     /gene="cstA"
                     /locus_tag="OO575_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6665817.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            13953..14150
                     /locus_tag="OO575_002931"
     CDS             13953..14150
                     /locus_tag="OO575_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6665818.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(14160..15248)
                     /gene="hcxA"
                     /locus_tag="OO575_002932"
     CDS             complement(14160..15248)
                     /gene="hcxA"
                     /locus_tag="OO575_002932"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6665819.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            15357..16517
                     /gene="ybdL"
                     /locus_tag="OO575_002933"
     CDS             15357..16517
                     /gene="ybdL"
                     /locus_tag="OO575_002933"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6665820.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
BASE COUNT         3657 a         4327 c         4741 g         3810 t
ORIGIN      
        1 aatagagtga atgttgcatt cagtggaagg aatttacaaa cttcagcttc ggataattct
       61 caccaactgg taaagtgagc gccgtggaga ttttccccta ttggactcat tttcaggtta
      121 taccccatgt catcattgaa tattaaacag ggaagtgacg ctcatttttc tgagtatcct
      181 ctggcgtcgc ccagtaataa tgaaattgat ttacttaatc taatcgaagt tttatggcgg
      241 gccaaaaaaa cggtcatggc ggtcgttttt gcatttgcct gcgcaggttt actgatctct
      301 ttcatcctgc cgcaaaaatg gaccagctca gccgttatta ctcccgccga agccattcag
      361 tggcaggacc tggagaaaac ctttaccaaa ctccgcgttc ttgatctcga tgttaatatc
      421 gaccgtggcg gtgcatttaa cctgtttatc aagaagtttc agtctgttag cttgctggaa
      481 gagtacctgc gttcatcacc ttatgtgatg gactaattaa aagaggcgaa aatcgatgaa
      541 ctggatttgc atcgcgctat tgttgcattg agcgaaaaaa tgaaagcggt tgatgacaat
      601 gctagtaaga aaaaagatga accgtcactg tatacatcct ggacgctaag ttttaccgcg
      661 ccaaccagta aagaggcgca gaccgttttg agcgggtata tcgattatat ctctgcgttg
      721 gtggtgaaag agtcgataga aaacgtccgt aataaactgg agatcaaaac ccagtttgaa
      781 aaagaaaaac tggctcagga tcgcattaaa acgaaaaatc aacttgatgc aaacattcag
      841 cgcctcaatt attcactcga cattgccaac gcggcaggaa ttaaaaagcc cgtatacagt
      901 aatggtcagg ccgttaaaga tgaccccgat ttctctattt ccctcggcgc agacggtatt
      961 gaacgcaaac tggaaattga aaaagcggtt actgacgttg cggaactgaa cggtgaatta
     1021 cgtaatcggc agtatcttgt cgagcaatta acaaaagcaa atgtcaacga tgtgaatttt
     1081 acgccgttta aatatcagtt aagcccgtca ttgccagtga aaaaagacgg tccgggtaag
     1141 gcgattattg tgatcctttc cgcgctgatc ggcgggatgg tggcttgtgg tagcgtgtta
     1201 ttgcgctatg cgatggcatc cagaaaacag gatgccatga tggcagacca cttagtttaa
     1261 ctatttgcgg ttgaaggtgc cgttcgtccg agcggcacca caagcggcat tccggctacc
     1321 ggatcgtcaa tgatcatgca tcgcagacca taaatgcgct caatcagttc agcagtgaca
     1381 atctccttcg gcgctccctg agcaacaatt ttcccttccc gcaatgcaat caaatggctg
     1441 gcgtaacgac aggcctgatt aagatcgtgc agcaccgccg ccagggtata gcctttctcg
     1501 cggttcagtt cgcttaacag ttccagtaga tcaatctgat gactgatatc cagccaggtc
     1561 gtgggttcgt cgagcagcat aattgccgtt tcctgggcca gcaccatcgc gatccacgct
     1621 cgctggcgtt gtccgccaga aagggtatcc acgctttgat ctgccagatg agttattccc
     1681 gtggcctgca tcgctttcgt taccgcttct tcatcctctt tgcgccagcg ggtaaacagc
     1741 ggttgatgcg gataacgtcc acgcgccacc agctcctgca cggtgatatc gcccggcgtg
     1801 gtagcatttt gcgccaacag accaatccgg cgtgcaactt ctttactggc gtaatgttga
     1861 atgtgctcgc catccagcca gacatgcccg tgagcaggcg tcatcaggcg gcttaaggta
     1921 cgcagtaacg tggatttacc acagccattt ggtccgataa ttgccgtgaa gtgaccatca
     1981 ggaatttcta cagtcagatt ttccgcaacg gtatatttgc catatcccag ggttaactgt
     2041 tcgccgcgca aacgggctac tgattcggtc attttttgcg agactcctga attaacaaga
     2101 cgataaggta aataccgccg aggctgacgg taacgacgcc aaccggaagt tgatacggca
     2161 taaacagttg ttgggcgcag agaacggccg ccagcagtaa cagcgctccg cacagcgccg
     2221 cctgggtcag cccccagcga gcggtgccgc taatgcgtcg ggcaatgtgc ggcgcgacta
     2281 aagcaataaa ggaaatcggc ccggcaagcg ccgttgccgc agcggtaagc accactgcaa
     2341 ccagcatcat taacagacgc gaacgctcga cgctgacgcc cagcgcgcag gcggtatcat
     2401 cgcccatctc cagcaagcgc attcgccgca ccagcaaggt ggcggcaatg agcatcaata
     2461 taatgatggg tgcggaaggc gaggtttttg cccacgtcag gccgttgagc gatccggcat
     2521 tccacaaacc tgctgttagc gccgtttcta aagacgcttt cagcaacagc caggtattaa
     2581 aggccaccag catggcgcga acgccgatac cgataataat caaccgaaag gtgtcgatac
     2641 cgttacgcca ggcgagcagc cagaccaaca gcgaagtgat aatgccgccc accattgctg
     2701 ccagcgcgat agccgtcaga tcctgaccaa acagcaccat cgccaccagc acgccgctcc
     2761 acgccccggt gttaaagccc attacgtcag ggctgccgag cgggttacgc atcagcgact
     2821 gaaaaatcgc gccgctgacg cccagcgccg cgccaatcaa cagcgccatc agcacgcgtg
     2881 gtaaacgcca ttcggtgacc accatcgtca tactgcgcgg cgcatcgccc atcaacgcgg
     2941 cgaatacctg cgagatttcc agcgtgacgg caccgctgcg taatccccag atacctgcaa
     3001 ccacacaggc ggaaatcagc agcaaacagg tgatgagtaa tcggcgagag acgtaaatca
     3061 tgcaccacct cgcgttttac gtcgcacgag gaagatcagc accggtgcac caataaacgc
     3121 actgaccaca gaaacgcgca gttcgccggg cacaatcacc cgcccgatga tatcggcaaa
     3181 cagcagcagg gcaggggtag caagtagcgt gacgggcagc gaccagcgat gatcggcacc
     3241 taccagccag cgcgccatgt gcggcatcat caggccgata aaggcaatcg ggccaactac
     3301 cgccgtcgca ctaccacaaa gcacggtaat cgccagcaga ccaatcaact gtgtgcgcgc
     3361 cacgcgactg cccagcgccg tcgcggtgtc actgccgagg ctcaaactgt tcagcgcgcg
     3421 actcagtaat agcgcagttg ctccggcgat cagaaccggg atcagcacca cttttaaggt
     3481 atgtagattg cgaatatcca gcgaaccggc ttgccagaaa cgcagttgat cgtagacgtc
     3541 aggattaagc agggcgatgc cgctggtcag tccttccagc accgccgcca gcgccacgcc
     3601 cgccagggtt aaacgcaccg gacttaactg ccctccaccc tgactgccgg taaaggcaac
     3661 aatcaatgag gccaccagcg ccccggcgaa ggccatcgcc agttgttcct gtgcggatga
     3721 gtaaccaaac agcgccgcac ccagcacaat ggcaaagctg gctccggcgt tcacgccaag
     3781 caagccgggg tcggcaagtg ggtttcgggt gagggtttgc attaacgccc cggcaaggcc
     3841 aagcgcgccg cctgccagta aaccggcaag ggtacgcggc agccgcgcgt cgagcacgat
     3901 ggtgcagtcg gcgctctggc aggtgccgga gaaggcctcc agcactacgg aagcggggag
     3961 tgattttgcc ccaatgagca ggcttaatgc cgtcgcgata atcagtaata acagcaatcc
     4021 gggcacggca atggcgcgtg tcacggcaac agaaccagat atacgaactt ccatgataat
     4081 gaaattaatt atcgttatcg atcttatttg gatatgttaa catgtgcagc ctaagaatag
     4141 gtatttaaaa tatttgatgg caaggcattg taatgaataa acaatcctgg ctgcttaacc
     4201 tcagcctgtt gaaaacgcac ccggcgtttc gcgcagtatt cctcgctcgt tttatctcaa
     4261 ttgtgtctct gggtttgctc ggcgtcgcgg tgccggtgca gatccagatg atgacccatt
     4321 ctacctggca ggtggggctt tcggtgacgc tgaccggcgg cgcgatgttt gttggcctga
     4381 tggttggcgg tgtgctggcg gatcgctatg aacgcaaaaa agtgattttg ctggcgcgcg
     4441 gcacctgtgg cattggcttc attggactgt gccttaatgc actgctgccg gagccgtcat
     4501 tgctggcaat ctatttactt ggtttatggg atggtttttt cgcatcactt ggtgttacgg
     4561 cgctactggc ggcaacatct gcactggtag ggcgtgaaaa cttaatgcag gccggggcga
     4621 tcaccatgtt gaccgtgcgc ctggggtcgg tgatttcgcc catgattggc ggtttattgc
     4681 tggcgaccgg cggcgtagcc tggaactacg ggctggcggc ggcgggcacg tttattacct
     4741 tgttaccgtt attaagcctt ccggagttgc caccgccacc gcagccgctt gagcatccgt
     4801 tgaaatcatt actggcagga tttcgttttc tgcttgccag cccgctgctg ggtggtttat
     4861 tgacgatggc gagcgcggtg ctggtactgt atccggcgct ggctgacaac tggcagatgt
     4921 cagcggcaca gattggtttt ctctacgcgg cgatcccgct cggcgcggct attggtgcgt
     4981 taaccagcgg gaagctggca catagtgcgc gaccagggtt attgatgctg ctctccacgc
     5041 tgggatcgtt cctcgccatt ggtctgtttg gcctgatgcc gatgtggatt ttaggcgtgg
     5101 tttgtctggc gctgttcggc tggctgagcg cggttagttc gttgctgcaa tatacaatgc
     5161 tgcaaacgca aaccccggaa gcgatgttag ggcggattaa cggtttgtgg acggcgcaga
     5221 acgtgacggg cgatgccata ggcgcggcgc tgctgggtgg tttgggcgcg atgatgacac
     5281 cggttgcttc cgcaagcgcg agcggttttg gtttgttgat tatcggcgtg ttgttattgc
     5341 tggtgctggt ggagttgcga cgttttcgcc agacgccgcc gcaggtgaca gcgtccgaca
     5401 gttaatgctt aaaacagcgc cttaagccgt tccagcactt gcatggcgct gtagtaatcc
     5461 aggcggaacg tctcggttcc cagcgcataa acctgcttgt tttgtaccgc aggcaggtgc
     5521 gcgagcagtg gattggcata aatcgcatcg gcatctttct gatcaccagc gaacaggaat
     5581 agcgattcac cgtttaaccc cgcagccaga ttttccccac caagctgaat gatgtcatgg
     5641 cgtttgccct ggctttggct ggcgtttaac cctgcgggca atttcgccag cgtaaagccg
     5701 agttgttcca gcatctgccc ttgtgctgat tccggcgtcc agagattggc actgtgtgca
     5761 gcggcggtat agacaatggc agtgaccggc tgcggcggta atttgatttg ctctttcgcc
     5821 gctgccagtt gcttatcaaa ctgtgcaatc cgctctgccg cttgtttctc atgcccggta
     5881 atttcgccaa gctgcgttaa cagcgactgc cagcttttgt cgtcgtaatt gatgattaat
     5941 gtcggggcga tggtggaaag ctgatcatac agcgccagcg ccgaatcccc gccggttgcg
     6001 ctaattaaaa tcagatccgg catttgcgcg gcaacggctt cggcgctcgg ttcgccgata
     6061 tagagccgtt gcagtttgcg ttctttcgcc accttgctcc actggcgtaa aaagccctgg
     6121 tcatctgcga cgcggttatt cggcgtggtc gcgccgctgg cgatcaccgg agcatcaatc
     6181 gccagcagtg atccggtcag ggtgacgctg gtggaaacaa tacgctgcgg ctggctttcc
     6241 agggtatgag tgccccggct gtcagtaatc tgacgcggcc agtcggcggc ctgaactacg
     6301 gctattcctg aaagcaaaag tcctgttaat agaagggcgt tgcggtagag cggggcgagt
     6361 ctcacaagtc agcttcctgt tattaataag gttaagggcg taatgacaaa ttcgacaaag
     6421 cgcacaatcc gtccctttgg ggagaggggg caaaacggcg caggacatca cattgcgctt
     6481 atgcgaattc atcaataatg cttctcattt tcattgtaac cacaaccaga tgcaaccccg
     6541 agttgcagat tgcgttacct caagagttga catagtgcgc gtttgctttt aggttagcgg
     6601 ccgaaaatat aagtgataat cattattaaa gcctttatca ttttgtggag gatgatatgg
     6661 atacgtcact ggctgaggaa gtacagcaga ccatggcaac acttgcgccc aatcgctttt
     6721 tctttatgtc gccgtaccgc agttttacga cgtcaggatg tttcgcccgt ttcgatgaac
     6781 cggctgtgaa cggggattcg cccgacagtc ccttccagca aaaactcgcc gcgctgtttg
     6841 ccgatgccaa agcgcagggc atcaaaaatc cggtgatggt cggggcgatt cccttcgatc
     6901 cacgtcagcc ttcgtcgctg tatattcccg aatcctagca gtcgttctcc cgtcaggaaa
     6961 aacagacctc agcccgctgt tttacccgca gccagtcgct gaacgtggtg gaacgctagg
     7021 caattcctga acaaaccacg tttgaacaga tgggtgcccg cgccgccgca cttaccgcca
     7081 cgccgcaggt cgacaaagtg gtgttgtcac gattgattga tatcaccact gacgccgcca
     7141 ttgatagtgg cgtattgctg gaacggttga ttgcgcaaaa cccggttagt tacaacttcc
     7201 atgtcccgct ggctgatggt ggcgtcctgc tgggggccag cccggaactg ctgctacgta
     7261 aagacggcga gcgttttagc tccattccgt tagccggttc cgcgcgtcgt cagccggatg
     7321 aagtgctcga tcgcgaagcg ggtaatcgtc tgctggcgtc agaaaaagat cgccatgaac
     7381 atgaactggg gactcaggcg atgaaagagg tactgcgcga acgcagtagt gagttacacg
     7441 ttccctcctc tccacaattg attaccacgc cgacgctgtg gcatctcgca actccctttg
     7501 aaggtaaagc gaattcgcaa gaaaacgcac tgactctggc ctgtctgctg catccgaccc
     7561 ccgcgctgag cggtttcccg catcaggccg cgacccaggt tattgctgaa ctggagccgt
     7621 tcgaccgcga actgtttggc ggcattgtgg gttggtgtga cagcgaaggt aacggcgaat
     7681 gggtggtgac catccgctgc gcgaagctgc gggaaaatca ggtgcgtctg tttgccggag
     7741 cggggattgt gcctgcgtcg tcaccgttgg gtgagtggcg cgaaacaggc gtcaaacttt
     7801 ctaccatgtt gaacgttttt ggattgcatt aaggagcgag gatgagcatt ccattcaccc
     7861 gctggccgga agagtttgcc cgtcgctatc gggaaaaagg ctactggcag gatttgccac
     7921 tgaccgacat tctgactcgc cacgctgcga gtgacagcat cgcggttatc gacggcgagc
     7981 ggcagttgag ttatcgggag ctgaatcagg cggcggataa cctcgcctgt agtttgcgcc
     8041 gtcagggcat taaacctggt gaaaccgcgc tggtacaact gggtaacgtc gctgaacttt
     8101 acattacctt tttcgcgctg ctgaaactgg gcgtcgcgcc ggtgctggcg ctgttcagcc
     8161 atcagcgtag tgaactgaac gcctacgcca gccagattga accggcgtta ctgattgccg
     8221 atcgccagca tgcgctattt agcggggatg atttcctcaa cacatttgtt gcagagcatt
     8281 cttccattcg cgtggtgcaa ctgcacaacg acagcggtga gcataacttg caggatgcga
     8341 ttaaccatcc ggcagagggt tttactgcca cgccatcacc tgctgatgaa gtggcctatt
     8401 tccagctttc cggcggcacc accggcacac cgaaactgat cccgcgcact cataacgact
     8461 attactacag cgtgcgtcgt agcgccgaga tttgtcagtt cacacaacag acgcgctacc
     8521 tgtgctcgat cccggcggct cataactaca ctatgagttc gccaggatcg ctgggcgtct
     8581 ttcttgccgg aggaacggtt gttctggcgg ccgatcccag cgccacgctt tgcttcccgt
     8641 tgattgcaaa acatcaggtt aacgttaccg cgctggtgcc gccggcagtc agcctgtggt
     8701 tgcaggcgct ggctgaaggc gaaagccggg cgcagcttgc ctcgctgaaa ctgttacagg
     8761 tcggcggcgc acgtctttct gccacgcttg cagcgcgtat tcccgctgag attggctgcc
     8821 agttgcagca ggtgtttggc atggcggaag ggctggtgaa ctacacccgt cttgatgata
     8881 gcgcggagaa aattatccat acccagggtt acccaatgtg tccggacgat gaagtgtggg
     8941 ttgccgatgc cgaaggaaat ccactgccgc aaggggaagt tggacgcctg atgacgcgcg
     9001 ggccgtacac cttccgtggc tattacaaaa gtccgcagca caatgccagt gcctttgatg
     9061 ccaacggttt ttactgttcc ggcgatctga tctctattga tccagagggt tacatcaccg
     9121 tgcaggggcg cgagaaagat cagatcaacc gtggcggcga gaagatcgct gccgaagata
     9181 tcgaaaacct gctgctgagc catccggcag tgatctatgc cgcactggtg agcatggaaa
     9241 atgagctgat gggcgaaaaa agctgtgctt atctggtggt aaaagagccg ctgcgcgcgg
     9301 tgcaggtgcg tcgtttcctg cgtgaacagg gtattgccga atttaaatta ccggatcgcg
     9361 tggagtgtgt ggattcactt ccgctgacgg cggtcgggaa agtcgataaa aaacaattac
     9421 gtcagtggct ggcgtcacgc gcatcagcct gaaggagaga acacgatggc tattccaaaa
     9481 ttacaggctt acgcactgcc ggagtctcac gatattccgc agaataaagt tgactgggcc
     9541 tttgaaccgc aacgtgccgc gttgttaatc catgatatgc aggactattt tgtcagcttc
     9601 tggggcgaga actgcccgat gatggagcag gtgatcgcga atattgctgc gctgcgcgac
     9661 tactgcaaac agcacaatat cccggtttat tacaccgccc agccgaaaga gcagagcgat
     9721 gaagatcggg cgctgttgaa tgatatgtgg gggccgggcc tgacccgctc gccggaacag
     9781 caaaaggtgg tggatcgcct gacgccagat gccgacgaca cggtgctggt gaagtggcgc
     9841 tacagcgcgt ttcatcgttc tccgctggag caaatgctca aagagagtgg acgtaaccag
     9901 ctgattatta caggagtata tgcccacatt ggctgtatga ccaccgcaac cgacgcattt
     9961 atgcgcgata ttaaaccgtt tatggtggcg gatgcgctgg ctgatttcag ccgtgacgag
    10021 catttgatgt cgctgaaata tgtggccgga cgttctggcc gggtggtgat gaccgaagaa
    10081 ttactgccag cacctatccc cgccagcaaa gcggcgctgc gtgaggtgat cctgccgttg
    10141 ctggacgagt ccgatgaacc gttcgatgac gacaacctga tcgactacgg tctggattcg
    10201 gtgcgcatga tggcgctggc ggcgcgctgg cgcaaagtgc atggtgatat cgactttgtc
    10261 atgctggcga aaaacccgac catcgacgcc tggtggaagc tactctcccg cgaggtgaaa
    10321 taatggattt cagcggtaaa aatgtctggg taaccggcgc aggtaaaggt atcggctacg
    10381 ccacggcgct gacgtttgtt gaggcgggag cgaaagttac aggttttgat caagcgttca
    10441 ctcaggagca atatcccttt gcgaccgaag taatggatgt tgccgacgcc gggcaggtgg
    10501 cgcaggtatg tcagcgactg ttagctgaaa cggagcgact ggacgtgctg atcaatgcgg
    10561 cgggaatttt gcgcatgggc gcgaccgatc agctcagtaa agaggactgg cagcagactt
    10621 ttgcggttaa cgtcggcggt gcgtttaacc tgttccagca aaccatgaac cagtttcgcc
    10681 gtcagcgggg cggggcgatt gtcactgtgg cgtccgacgc cgcacacacg gcgcgtattg
    10741 gtatgagtgc ttatggcgca tcgaaagcgg cgctgaaaag cctggcgttg agcgtcgggc
    10801 tggaactggc gggtagcggc gtgcgctgta atgtggtttc gcctggctcc accgacaccg
    10861 atatgcaacg cacgctgtgg gtgagcgatg acgccgaaga acagcgtatt cgcggctttg
    10921 gcgagcagtt taaactcggc attccgctgg ggaaaatcgc ccgtccacaa gagatcgcca
    10981 acacgatttt gttcctcgcc tctgacctcg ccagccatat caccctacag gatattgtgg
    11041 tcgatggcgg ctcaacgctg ggggcataag catgatctgg aaacgccatt taacgctcga
    11101 cgaactgaac gccaccagcg ataacacaat ggtggcgcat ctgggaattg tgtataccct
    11161 tctgggagat gatgtgctgg aagccgaaat gccggttgat acccgtactc atcagccgtt
    11221 tggcctgctg catggcggcg cgtcggcggc gctggctgaa acgctgggat cgatggccgg
    11281 atttatgatg acccgtgacg ggcagtgtgt ggtgggcacg gaacttaacg ccacccatca
    11341 tcgcccggtg tctgaaggca aggtacgcgg cgtctgccag ccgctgcatc ttgggaggca
    11401 aaatcagagt tgggaaatcg tcgttttcga tgaacagggg cggcgttgct gcacttgtcg
    11461 gctgggtacg gcagttttgg gatgagcccg acagaattaa atgagattgc aggaaaactc
    11521 ggttaacgga gtgatcgagt taacattgtt aagttaaata ttggtttcaa ctccgattta
    11581 catggttacc gtgttgttaa atcgtacaaa gatgttatag aaacaaaatg taacatctct
    11641 atggacacgc acacggataa caactatgaa caaatcaggg aaatacctcg tctggacagt
    11701 gctctctgta atgggagcat ttgctctggg atacattgct ttaaatcgtg gggaacagat
    11761 caacgcgctg tggattgtgg tggcgtcggt ctgtatctat ctgatcgctt accgttttta
    11821 tgggctgtat atcgccaaaa atgtgctggc ggttgacccg acgcgtatga cgccagcggt
    11881 gcgccataac gacgggctgg actatgtgcc gacggacaag aaagtgctgt tcggtcacca
    11941 ttttgcggcc attgccggag cagggccgct ggtggggccg gtgctggcag cgcaaatggg
    12001 ctacctgccg gggatgatct ggctgctcgc cggggtggtt ctcgccggtg cggtgcagga
    12061 tttcatggtg ctgtttgttt ctacgcgccg tgacggtcgc tcgctgggtg agctggtcaa
    12121 agaagagatg gggccaactg ccggggtgat tgcgctggta gcctgcttta tgatcatggt
    12181 cattatcctt gcagtactgg cgatgatcgt ggtgaaagcc ctgactcata gcccgtgggg
    12241 aacgtatacc gttgcgttca ccattccgct ggcgattttt atgggaatct acctgcgcta
    12301 tctgcgtccg ggccgcattg gcgaagtgtc agtgattggc ctggtgttcc tgattttcgc
    12361 cattatctct ggcggctggg tggcagaaag tccgacctgg gcaccgtact ttgactttac
    12421 cggcgtgcag ctgacctgga tgctggtggg ctacggtttt gtggcggcgg tgctgccggt
    12481 gtggttgctg ctggccccgc gtgactatct ctcgaccttc ctgaaaatcg ggactatcgt
    12541 cggtctggcg gtaggcattt tgattatgcg cccgacgctg accatgcctg cgctgaccaa
    12601 atttgtcgat ggcactggcc cggtatggac cggtaacctg ttcccgttcc tgtttatcac
    12661 catcgcctgt ggtgcggtgt ctggcttcca tgcgctgatc tcttccggca ccacgccgaa
    12721 gatgctggcg aacgaagggc aggcgtgctt tatcggctac ggtgggatgt tgatggaatc
    12781 cttcgtggcg attatggcgc tagtttctgc ctgtatcatc gatccgggcg tgtattttgc
    12841 catgaacagc ccaatggcgg tgctggctcc ggcagggacg gcggatgtag tcgcttctgc
    12901 cgcgcaggtg gtgagtagct ggggctttag cattacacca gatacgctca accagattgc
    12961 cagcgaagtg ggtgaacagt cgatcatttc ccgtgcgggc ggtgcgccga cgttggcggt
    13021 ggggatggct tacatcctgc acggtgcgct gggtggtatg atggatgtgg cgttctggta
    13081 tcacttcgcc attctgtttg aagctctgtt tattctgacg gcggtagatg cgggtacgcg
    13141 tgctgcgcgc tttatgttgc aggatctgct gggcgtggtg tctcctggcc tgaagcggac
    13201 cgattcactg cctgctaacc tgctggcaac agcgctgtgc gtactggcgt ggggctactt
    13261 cctccatcag agcgtggtcg atccgctggg cggtattaac actctgtggc cgctgtttgg
    13321 tattgccaac cagatgctgg cagggatggc gctgatgctc tgtgccgtgg tgttgttcaa
    13381 gatgaaacgt caacgttacg cctgggtggc gctggtacca acggcctggc tgctgatttg
    13441 taccctgacc gctgactggc tgaaagcgtt tagcccggat gcgaaagtcg gcttcctggt
    13501 cattgctaac aagttccagg caatgatcga cagcggcaat attccgtcgc agtatactga
    13561 gtcacagctg gcgcaactgg tgttcaacaa ccgtctggat gccgggttaa ccatcttctt
    13621 tatggtggtc gtggtggtcc tggcactgtt ctcgattaag acggcacttg cggcattgaa
    13681 agatccgaag ccaacggcga aagaaacgcc gtatgagccg atgccggaaa atgtcgagga
    13741 gatcgtagcg caggcgaaag gcgcgcacta aagtcagagt gaggggggat gttggcgaat
    13801 gttggcatag tgccgagggt tccccctcac cctaaccctc tccccgatgg ggcgagggga
    13861 ctgaccgagt gcgttgatag catttgtagg ccggataaag cgttcacgcc gcatccggca
    13921 ctctttcagc aacatggtta gcggaggcca agatgtttga ttcactggca aaagccggaa
    13981 aatatttagg tcaggcggcg aagctgatga ttggtatgcc tgattacgac aactatgtcg
    14041 aacatatgcg ggttaatcat cccgatcaaa cgccgatgac ctacgaggag tttttccgtg
    14101 agcggcagga cgcgcgctac ggtggaaaag gcggcgcgcg ctgctgctaa atttgcacgt
    14161 caggctttaa acgattccac tttttcgaac gctgcacgca acgtatctgg tgtcagcgtg
    14221 actggcaggt aatgaatgga ctccaccggg cgcaaggtgt gggcaatcac tttgtcgatc
    14281 tccgcctgat tattgatatc cacctccagc tccgccagcg tagtcggcag atgaaaacgc
    14341 tgatacgccc cggttaattg cgccagcaca tcatcctgac ccagcaaggc gctttgcacc
    14401 agaattccgt aagcgacttt ggtgccgtga agaaacttct cggtttgcgg caggacagtc
    14461 agaccgttat gcacggcatg agccgccgcc acacgcgtaa aacgatcgcc cagaccaccg
    14521 accatcccac caccagcaat aatagcatcc accacatcgc aaaatgattg cgttaactgc
    14581 tgattttgct gatcggcaag tgcctgttcg ctactgttta acaagacgtc gcgaatggcc
    14641 tgcgcattat tgatccccag ccgcacggtt agcggcaacg tttctggttg cggagccagc
    14701 accaccgctt cataccattt cgccagcgtg tcaccgatcc ccgccagcag atattgttgc
    14761 ggtgcattga ggataatctc cggttccacc agcaccataa aattggcgtc gtcgaaaatc
    14821 tcataatgca gcgcctgtcc ggcatcgtta taccagacgg agagcggtgt ccaggctgcg
    14881 caggtggcgg cgatcgtcgg agcggcaaca aatggcagac cgagacggcg ggcgagggct
    14941 ttcgcggtgt cgagcagtgc gccgccgccg acgccaatca ccacgctgcg atcgtcaccg
    15001 gactcagccg ccagttgctg tacatcgctt tcgctgcaat gaccacgaaa caaaatatgc
    15061 tttgcccctg gaagtccaaa cgctggcgga agtttggttt gcgccgcagc aatggcgcgt
    15121 ttgccgtaga tccacaccgc gcgagaaagt tgttcatcag tgaaaaaatt gtgcaggtga
    15181 ttgaaacttc ctggatgtga aaagtagtta gccgggccga cgaccacgcg gataggattg
    15241 tgaggcatgt tgtgttgtcc ttattgtttt atttagacat ctaaacgtct tgattgccaa
    15301 atactggcat cgtgttatag tgccttcaac acgcaacttc gtcaggtaca ataaaaatga
    15361 caaataaccc tctgattcca caaagcaaac ttccacaact tggcaccact attttcaccc
    15421 agatgagcgc gctggcgcag caacaccagg cgattaacct gtcgcaaggc tttcctgatt
    15481 ttgatggtcc gcgctattta caggagcggc tggcgtacca cgttgcccag ggggcaaacc
    15541 aatacgcgcc catgaccggc gtgcaggcct tgcgcgaggc gattgctcag aaaacggaac
    15601 gtttgtatgg ctatcaacca gatgccgata gcgatatcac cgtaacggca ggggcgacgg
    15661 aagcgttata cgcggcgatt accgcactgg tgcgcaatgg cgatgaagtg atttgttttg
    15721 atcccagcta tgacagttac gcccccgcca tcgcgctttc tgggggaata gtgaaacgta
    15781 tggcactgca accaccgcat tttcgcgttg actggcagga atttgccgca ttattaagcg
    15841 agcgcaccag actggtgatc ctcaacactc cgcataaccc cagtgcaact gtctggcagc
    15901 aggctgattt cgccgctttg tggcaggcga tcgccgggca cgagattttt gtcattagcg
    15961 atgaagtcta cgagcacatc aacttttcac aacagggcca tgccagtgtg ctggcgcatc
    16021 cgcagctgcg tgagcgggca gtggcggttt cttcatttgg caagacctat catatgaccg
    16081 gctggaaagt gggttattgt gttgcgccag cgcccatcag cgccgaaatt cgcaaggtac
    16141 atcagtatct gaccttttcg gtgaataccc cggcacagct ggcgcttgct gatatgctac
    16201 gtgcagaacc tgagcattat cttgcgttac cggactttta tcgccagaag cgcgatattc
    16261 tggtgaatgc tttaaatgaa agccggctgg agattttacc gtgtgaaggt acatactttt
    16321 tgctggtgga ttacagcgcg gtttctaccc tggatgatgt tgagttttgc cagtggctga
    16381 cgcaggagca cggcgtagcg gcgattccgc tgtcggtgtt ttgcgccgat cccttcccac
    16441 ataaactgat tcgtctctgt tttgccaaga aggaatcgac gttgctggca gcagctgaac
    16501 gcctgcgcca gctttagcta tttaaccgtc caggc
//
LOCUS       DAJTYM010000086        15746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTYM010000086 DAJTYM010000000
VERSION     DAJTYM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1588)
                     /gene="speA"
                     /locus_tag="OO575_002934"
     CDS             complement(<1..1588)
                     /gene="speA"
                     /locus_tag="OO575_002934"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6665821.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCD"
     gene            complement(1597..1728)
                     /gene="yqgB"
                     /locus_tag="OO575_002935"
     CDS             complement(1597..1728)
                     /gene="yqgB"
                     /locus_tag="OO575_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6665822.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            1864..2079
                     /locus_tag="OO575_002936"
     CDS             1864..2079
                     /locus_tag="OO575_002936"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665823.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2383..3537
                     /gene="metK"
                     /locus_tag="OO575_002937"
     CDS             2383..3537
                     /gene="metK"
                     /locus_tag="OO575_002937"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6665824.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            3978..5372
                     /gene="galP"
                     /locus_tag="OO575_002938"
     CDS             3978..5372
                     /gene="galP"
                     /locus_tag="OO575_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6665825.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5449..5946
                     /locus_tag="OO575_002939"
     CDS             5449..5946
                     /locus_tag="OO575_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6665826.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6041..6748
                     /gene="endA"
                     /locus_tag="OO575_002940"
     CDS             6041..6748
                     /gene="endA"
                     /locus_tag="OO575_002940"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6665827.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            6828..7559
                     /gene="rsmE"
                     /locus_tag="OO575_002941"
     CDS             6828..7559
                     /gene="rsmE"
                     /locus_tag="OO575_002941"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6665828.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7572..8522
                     /gene="gshB"
                     /locus_tag="OO575_002942"
     CDS             7572..8522
                     /gene="gshB"
                     /locus_tag="OO575_002942"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6665829.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            8631..9194
                     /locus_tag="OO575_002943"
     CDS             8631..9194
                     /locus_tag="OO575_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6665830.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9194..9610
                     /gene="ruvX"
                     /locus_tag="OO575_002944"
     CDS             9194..9610
                     /gene="ruvX"
                     /locus_tag="OO575_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6665831.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(9786..10766)
                     /locus_tag="OO575_002945"
     CDS             complement(9786..10766)
                     /locus_tag="OO575_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6665832.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            10784..11488
                     /gene="yggS"
                     /locus_tag="OO575_002946"
     CDS             10784..11488
                     /gene="yggS"
                     /locus_tag="OO575_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6665833.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11506..12072
                     /gene="yggT"
                     /locus_tag="OO575_002947"
     CDS             11506..12072
                     /gene="yggT"
                     /locus_tag="OO575_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6665834.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12069..12359
                     /locus_tag="OO575_002948"
     CDS             12069..12359
                     /locus_tag="OO575_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6665835.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12367..12960
                     /locus_tag="OO575_002949"
     CDS             12367..12960
                     /locus_tag="OO575_002949"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6665836.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            12953..14089
                     /gene="hemW"
                     /locus_tag="OO575_002950"
     CDS             12953..14089
                     /gene="hemW"
                     /locus_tag="OO575_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6665837.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14403..15366
                     /locus_tag="OO575_002951"
                     /pseudo
     CDS             14403..15366
                     /locus_tag="OO575_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15411..>15746
                     /locus_tag="OO575_002952"
     CDS             15411..>15746
                     /locus_tag="OO575_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6665838.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKL"
BASE COUNT         3872 a         4074 c         3986 g         3814 t
ORIGIN      
        1 agtcacaggt aatatccagc agcacagcgc gacgttccgg cacttgatcc agcccttcca
       61 gcggtagaac cgggaacaac tggtcgatcc cccatgcgtc cggcattgac tggaacagcg
      121 agaaattgac gtacattttg tccgccatac gttcctgcag ttcgtcgatt atcggacgat
      181 gcgcacggtt ttgcggatcc agttgcttct gcacttcatg gcacatgctc agatagagtt
      241 gttctgccca tgcacgttct tgcaggctaa aggtgccgga agagtagccg atatgaatgt
      301 cgtgcagatc catctggctg tcgtgtaacc attcacgcaa agaacggcga gttcccggtt
      361 cgtgcatttc ctgccaggtt tcccacatgc tttgcagcgc acgcggcgca tcttctacgg
      421 gcgcggtcgg caccgtatat tcgttacgtt ccacgccgat gatattcgac accagcacgg
      481 tatggtgcgc agtcaccgca cggcccgatt cggtgattac cgtcggatgc ggcagaccat
      541 tttcttcaca cgcatcgcca atcgcccaga tgatgttgtt ggcgtattca ttgaggccat
      601 agtttacgga gcagtcagac tgcgaacggg tgccttcata atccacgccc agaccgccgc
      661 cgacgtcgaa gcactgaata ttgacgccca gcttgtgcag ttccacatag aaacgcgcgg
      721 attcacgaac gcctgtcgcg atatcgcgaa tattcgccat ctgcgaaccg aggtggaagt
      781 gcagtagttg caggctgtcg agacgcccgg cttcacgcag ggtttcaacc agttgcagta
      841 cctgagtcgc agccaggccg aacttcgatt tttccccgcc ggaggactgc catttacccg
      901 agccctgcga agccagacgt gcacgcacgc ccagacgagg aacgacattc agacgttctg
      961 cttcatccag cacaatggcg atttctgaca tcttctcaat gaccagatag accttgtgcc
     1021 ccatcttctc gccaattaat gccaggcgga tatattcgcg gtctttataa ccgttgcaga
     1081 cgatgacgct acgagtcatg ccagcatgtg ccagcactgc catcaactcg gctttggaac
     1141 cggcttccag acccagcggt tcacccgaat gaatcaggga ctcgatcacg cggcggtgct
     1201 ggttaacttt gatcggataa acaaggaagt aatcgccgtt atagccgtag gattccctcg
     1261 cacgtttgaa cgcggcgtta atggaacgca aacggtgctg caggatctgt gggaaacaga
     1321 acagtgcagg cagacgctga ccctgtgctt cacgagtttt cactaactgc gcgagatcga
     1381 cgcgagcttc cgggacgtcc gggtccgggc acacgctgat gtggcccagc tcgttaacgt
     1441 catagtagtt attgccccac caggcaatat tgtaagtacg cagcatcttg ctggcttcct
     1501 gggagctcat tgcaacctcc tgcatggagc gtagtacacc gtgttcgccc gctgacgaag
     1561 gcaaacccat agacatgtcg tcagacatag cgaacctcaa attattttat taagtgtaaa
     1621 acagttaacg actgtcgcag cctggaactg cgataacaac ccataagcac atggattttc
     1681 cagcagtgta tgctgacgct ccgactgcgc aaccggtttc tttttcataa cattattaag
     1741 cacataaccg aacgtaagtg tgacagttcg gcgaaaccac gagaaaactc ttgtttttac
     1801 aagagcgccc ttgttcagtc ctcagtaact gtaaccagct cttgaatcct gagaagcgcc
     1861 gagatgggta taacatcggc aggtatgcaa agcagagatg cagagtgcgg ggaacgagtc
     1921 ttcaccagaa cggtgagaca ggttaagcag cagacaacgg ttcattattt cgtatcacct
     1981 ccacggccgc ctgttaagac gaacccacaa gccaaaactc tgatttcaac ccggctggaa
     2041 gtggcaacac gaaagaaacg tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac
     2101 cccacaatgg cagaaaattg caaaagataa atacgcagaa tgccggcatt gtcaggaaaa
     2161 atttccagcc acgtttttaa cagaatgaga cacgattcaa aaaaaagtgg aaatcgggtg
     2221 aagaattgac ctaaaatagc catccagatg ttaatccatc cataccgatt aacactcaga
     2281 ctgccagtgt ttttaacctg cagagtcgtg gtaggatccg ctaccacaga aaatccacac
     2341 aacagtttga gctaaccaaa ttctctttag gtgatattaa atatggcaaa acaccttttt
     2401 acgtccgagt ccgtctctga agggcatcct gacaaaattg ctgaccaaat ctctgatgcc
     2461 gttttagacg cgatcctcga acaggatccg aaagcacgcg ttgcttgcga aacctacgta
     2521 aaaaccggca tggttttagt tggcggcgaa atcaccacca gcgcctgggt agacatcgaa
     2581 gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtgc attccgacat gggctttgac
     2641 gctaactcct gtgcggttct gagcgctatc ggcaaacagt ctcctgacat caaccaaggc
     2701 gttgaccgtg ccgatccgct ggaacagggc gcgggtgacc agggtctgat gtttggctac
     2761 gcaactaatg aaaccgacgt gctgatgcca gcacctatca cctatgcaca ccgtctggta
     2821 cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa
     2881 agccaggtga ctttccagta tgacgacggc aaaatcgttg gtattgatgc tgtcgtgctt
     2941 tccactcagc actctgaaga gatcgaccag aaatcgctgc aagaagcggt aatggaagag
     3001 atcatcaagc caattctgcc cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac
     3061 ccgaccggtc gtttcgttat cggtggccca atgggtgact gcggtctgac tggccgtaaa
     3121 attatcgttg atacctacgg cggcatggcg cgtcacggtg gcggtgcatt ctctggtaaa
     3181 gatccatcaa aagtggaccg ttccgcagcc tacgcagcac gttatgtcgc gaaaaacatc
     3241 gttgctgctg gcctggccga tcgttgtgaa attcaggttt cctacgcaat cggcgtggct
     3301 gaaccgacct ccatcatggt agaaactttc ggtactgaga aagtgccttc tgaacaactg
     3361 actctgctgg tacgtgagtt cttcgacctg cgcccatacg gactgattca gatgctggat
     3421 ctgctgcacc cgatctacaa agaaaccgca gcatacggtc actttggtcg tgaacatttc
     3481 ccgtgggaaa aaaccgacaa agcgcagctg ctgcgcgatg ctgccggtct gaagtaatct
     3541 ttcttcacct gcgttcaaag gccagcctcg cgctggcctt tttcttttac ctcaccgcgg
     3601 tggataggcg ttcacgccgc atccggcaaa aaaactgccc gcacaataac atcattcttc
     3661 ctgatcacgt ttcacggcaa attaccatca caactgaaac cgattacacc aaccacacca
     3721 gcaaaatatt tgtaatattt tcatattatt attcagcttt cacagttgtt acatttcttt
     3781 tcagtaaagt cttacatcat tgcagatgac agcgtttaat ctatgatgat ataactcaat
     3841 tattttcatg cccttaaatc ataactaaga taaatgttag tgtaagcgat tacactgatg
     3901 tgatttgctt cacatctttt tacgtcgtac tcacctatct taattcacaa taaaaaataa
     3961 ctatattgga gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac aaggcaatga
     4021 cgtttttcgt ctgcttcctt gccgctctgg cgggattact ctttggcctg gatatcggtg
     4081 taattgctgg cgcactgccg tttattgcag atgaattcca gataacttcg cacacgcaag
     4141 aatgggtcgt aagctccatg atgttcggtg cggcagtcgg tgcggtgggc atcggctggc
     4201 tctcctttaa actcgggcgc aaaaagagcc tgatgatcgg cgcaatcctg tttgttgccg
     4261 gttcactgtt ctctgcggct gcgccaaacg ttgaagtact gattctttcg cgcgttctgc
     4321 tggggctggc ggtgggtgtg gcctcttata ccgcaccact gtacctgtca gaaattgcgc
     4381 cggaaaaaat tcgcggcagt atgatctcga tgtatcagtt gatgatcact atcgggatcc
     4441 tcggtgctta tctttctgat accgccttca gctacaccgg tgcatggcgc tggatgctgg
     4501 gtgtgattat catcccggca attttgctgc tgattggtgt cttcttcctg ccagacagcc
     4561 cacgttggtt tgccgccaaa cgccgttttg ttgatgccga acgcgtgctg ctacgcctgc
     4621 gtgacaccag cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt ttgcaggtta
     4681 aacagagtgg ctgggcgctg tttaaagaga atagcaactt ccgccgcgcg gtgttccttg
     4741 gcgtactgtt acaggtaatg cagcaattca ccgggatgaa cgtcatcatg tattacgcgc
     4801 cgaaaatctt cgaactggcg ggttatacca acaccaccga gcaaatgtgg gggacagtga
     4861 ttgtcggcct gaccaacgta cttgccacct ttatcgcaat cggccttgtt gaccgctggg
     4921 gacgtaaacc aacgctaacg ctgggcttcc tggtgatggc tgctggcatg ggcgtactcg
     4981 gtacaatgat gcatatcggt atccactctc cgtcggcgca gtatttcgcc atcgccatgc
     5041 tgctgatgtt tattgtcggt tttgccatga gtgccggtcc gctgatttgg gtactgtgct
     5101 ccgaaattca gccgctgaaa ggccgcgatt ttggcatcac ctgctccacc gccaccaact
     5161 ggattgccaa catgatcgtt ggcgcaacgt tcctgaccat gctcaacacg ctgggtaacg
     5221 ccaacacctt ctgggtgtat gcggctctga acgtactgtt tatcctgctg acattgtggc
     5281 tggtaccgga aaccaaacac gtttcgctgg aacatattga acgtaatctg atgaaaggtc
     5341 gtaaactgcg cgaaatcggc gctcacgatt aatctcctca ggcttcctcc cgtcgcggag
     5401 gaagccgcct cttgcagtca tcttttcttc gctctatcct ctgccgctat gaaaacatcc
     5461 cgtctcccta tcgccatcca acaggccgtt atgcgccgcc tgcgggaaaa actcgcccag
     5521 gccaacctga agctagggcg taactacccg gagccaaaac tctcttacac ccagcgcgga
     5581 acctccgccg gaacagcctg gctggaaagc tatgaaatcc gcctcaatcc cgttttgctg
     5641 ttggaaaaca gtgaagcttt tattgaagaa gtggtaccgc acgaactggc acatttgctg
     5701 gtatggaaac atttcggccg cgtagcgcca catggcaaag agtggaagtg gatgatggaa
     5761 agcgtgctgg gcgttcccgc ccgtcgtacg catcagttcg aactgcaatc cgtgcgtcgc
     5821 aacaccttcc cctaccgctg taagtgccag gaacatcagc ttaccgtacg ccgccataat
     5881 cgcgtagttc gtggcgaggc catctatcgc tgtgttcact gcggtgaaca gctggttgcg
     5941 aaataaccac ctgaactatc aggaactttc ctgatctggc tgattgcata ccaaaacaac
     6001 tttcgctacg ttgctggctc gttttaacac ggagtaagtg atgtaccgtt atttgtctat
     6061 tgctgcggtg gtactgagcg cagcattttc cggcccgacg ttggccgaag gtatcaatag
     6121 tttttctcag gcgaaagccg cggcggtaaa agtccacgct gatgcgcccg gtacgtttta
     6181 ttgcggatgt aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc aatcgtgcgg
     6241 ctatcaggtg cgcaaaaatg aaaaccgcgc cagccgcgta gagtgggaac acgtcgttcc
     6301 cgcctggcaa ttcggtcacc agcgccagtg ctggcaggac ggtggacgta aaaactgcgc
     6361 taaagatccg gtctatcgca agatggaaag cgatatgcat aacctgcagc cgtcagtcgg
     6421 cgaggtgaat ggcgatcgcg gcaattttat gtacagccag tggaatggcg gcgaaggcca
     6481 gtacggtcaa tgcgccatga aggtcgattt caaagaaaaa gctgccgaac caccagcgcg
     6541 tgcacgcggt gccattgcgc gcacctactt ctatatgcgc gaccaataca acctgacact
     6601 ctctcgccag caaacgcagc tgttcaacgc atggaacaag atgtatccgg ttaccgactg
     6661 ggagtgcgag cgcgatgaac gcatcgcgaa ggtgcagggc aatcataacc cgtatgtgca
     6721 acgcgcttgc caggcgcgaa agagctaacc tacactagcg ggattctttt tgttaacccc
     6781 taccccacgc gtacaaccgc gtggggagac gacgcggatt tttaactatg cgtatccccc
     6841 gcatttatca tcctgaacca ctgaccagcc attctcacat cgcgctttgc gaagatgccg
     6901 ccaaccatat cgggcgcgta ctgcgcatgg ggccggggca ggcgttgcaa ttgtttgacg
     6961 gtagcaacca ggtctttgac gccgaaatta ccagcgccag caaaaaaagc gtggaagtga
     7021 aggtgctgga aggccagatc gacgatcgcg aatctccgct gcatattcac ctcggtcagg
     7081 tgatgtcgcg tggtgaaaaa atggaattta ctatccagaa atcgatcgaa ctcggtgtaa
     7141 gcctcattac gccacttttt tctgagcgct gcggcgttaa actggatagt gaacgtctga
     7201 acaagaagct tcagcagtgg cagaagattg caattgctgc ctgtgagcag tgtggtcgta
     7261 accgggtgcc ggaaatccgt ccagcgatgg atctggaagc ctggtgtgca gagcaggatg
     7321 aaggactgaa actgaatctt cacccgcgcg ccagtaacag catcaatacg ttgcctttac
     7381 cggttgaacg cgtccgcctg ctgattggcc cggaaggcgg tttatcggca gatgaaattg
     7441 ccatgactgc ccgctatcaa tttactgata tcctgttggg acctcgcgtt ttgcgtacag
     7501 agacaactgc gctcaccgcc attaccgcgc tacaagtacg atttggcgat ttgggctaac
     7561 ggagaagaat aatgatcaag ctcggcatcg tgatggaccc catcgcaaac atcaacatca
     7621 agaaagattc cagtttcgct atgttgctgg aagcacagcg tcgtggttac gaacttcact
     7681 atatggagat gggcgatctg tatctgatca atggcgaagc ccgcgcccat acccgcacgc
     7741 tgaacgtgaa gcagaactac gaagagtggt tttcgttcgt cggtgaacag gatctgccgc
     7801 tggccgatct cgatgtgatc ctgatgcgta aagacccgcc gtttgatacc gagtttatct
     7861 acgcgaccta tattctggaa cgtgccgaag agaaagggac gctgatcgtt aacaagccgc
     7921 agagcctgcg cgactgtaac gagaaactgt ttaccgcctg gttctctgac ttaacgccag
     7981 aaacgctggt tacgcgcaat aaagcgcagc taaaagcgtt ctgggagaaa cacagcgaca
     8041 tcattcttaa gccgctggac ggtatgggcg gcgcgtcgat tttccgcgtg aaagaaggcg
     8101 atccaaacct cggcgtgatt gccgaaaccc tgactgagca tggcacttgc tactgcatgg
     8161 cgcaaaatta cctgccagcc attaaagatg gcgacaaacg cgtgctggtg gtggatggcg
     8221 agccggtacc gtactgcctg gcgcgtattc cgcagggggg cgaaacccgt ggcaatctgg
     8281 ctgccggtgg tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa atcgcccgtc
     8341 agatcgggcc gacgctgaaa gaaaaagggc tgatttttgt tggtctggat atcatcggcg
     8401 accgtctgac tgaaattaac gtcaccagcc caacctgtat tcgtgagatt gaagcagagt
     8461 ttccggtgtc gatcaccgga atgttaatgg atgccatcga agcacgttta cagcagcagt
     8521 aacccacctt agcgagaagg atctcgttga gactctgagt gacagcgccc ttctttccac
     8581 gcatactggg cgctgttgct tttttgaacc aggaaacaga acctctgaca atgaatttac
     8641 agcatcactt tcttattgcc atgcctgctc tccaggatcc gattttccgt cgttccgtgg
     8701 tctacatttg cgaacataat accaatggtg caatggggat catcgtcaac aagccgctgg
     8761 aaaatctcaa aattgaaggg attctggaaa agctgaagat cacgccggag ccgcgtgatg
     8821 aatcaatccg tctggataaa ccggttatgc tcggcggccc actggctgaa gatcgcgggt
     8881 ttattttgca tactccgccc tccaattttg cttccagcat tcgcatttca gacaacacgg
     8941 tgatgaccac ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa ccgtctgacg
     9001 tattggtggc tctgggctat gcctcctggg aaaaaggtca actggaacaa gaaattctcg
     9061 ataacgcgtg gctaacggcc ccggcggatc tgaatattct gttcaaaacg ccgattgccg
     9121 accgctggcg cgaggcggca aaactgattg gtgtggatat tctcaccatg cctggtgtgg
     9181 caggacacgc ctgatgagtg gaaccttact cgccttcgac ttcggcacca aaagcattgg
     9241 cgtagcggtc ggccaacgca ttaccggcac cgctcgccct ttgcctgcaa ttaaagcgca
     9301 ggacggtacg ccggactgga acctcatcga acgtttactg aaagagtggc agcctgacga
     9361 aatcatcgtc ggtttgccgc tgaatatgga cggcaccgaa cagccgttaa ccgccagagc
     9421 gcgcaaattt gccaaccgta ttcatggtcg tttcggtgtt gaagtaaagc tccatgacga
     9481 gcgtcttagc actgtggaag cccgttccgg tctttttgaa cagggcggct atcgggcgct
     9541 caacaaaggc aaagttgact ctgcctctgc ggttattatt ctcgaaagct atttcgagca
     9601 gggatattaa ggcgatctaa aagcctggcg gatagtcgtt accgccatct tgccggatgc
     9661 ggcgtgaacg ccttatccag cacacatctg gcagcggctg taggtctgat aagacgcgtt
     9721 agcgtcgcat cagacattta ttgcctttat aggccctata aacttgcgca tcgggcatgg
     9781 caacgtcaca aacgcccttc ccccacccgc tgctgataac tctgctgaaa cgttaacatc
     9841 cccaccttct gcccggtttg aataacatgc ggtaactggt gggttttccc ttcgcgaatc
     9901 aaattcccca ccgcgggagt gttaatcagt aattcaaaca gcgccacgcg tccttcctgt
     9961 ttatccactt ccagcttttg tgacagcact gcccgtaaac tacccgcaag ttgattacgt
    10021 acgggatctt tttcctgcgc cggaaatgaa tccaccagtc gctcaactgc ctgcgcggca
    10081 ccacgtgtat gtaaggttgc cagcaccaga tgcccggttt ctgccgccgt cagcgccaga
    10141 cggattgtct cactgtcacg tagctctccg agcaaaatca catcaggatc ttcccgcaat
    10201 gcagcccgca accccgatgc gaacgtcata cagtgcaaac caatttcccg ctgctggatc
    10261 aaacatcgct ggctggcata gagatattcc acaggatctt ccagcgtcag aatatgcgca
    10321 tcggcatgtt gattaagata gccaaccatc gccgccagcg tggtagattt gccactcccc
    10381 gtagcccccg tcaccagaat caggccattc tcgctcttga gtaattccgg caataccggt
    10441 ggtgcaccaa gctgttcgag ctgtggacag tgcgaaggta acaaccgtaa tgccagagaa
    10501 ataccttgcc gttgcgcgaa cgcactgcca cgcaaccgct ggttttccgc cagcgacacg
    10561 gcaaaatcca gctggccatt ttccagcaat attgcccgct gatcgtcatc cagccactcc
    10621 cgcagtagct cttcgacgtc cggcgcatca aacggcgcag cttccattct gccacgaatg
    10681 cgccatcgtg cgggccaggc gctgcacagg tgtagatccg agacgttatg ctttacacta
    10741 agggccacaa tttcttccat attcatacta agatcctcgg aaaatgaacg atattgcgca
    10801 taacctggca caggtccggg acaaaatctc agcggctgca acgcgttgcg gccgttctcc
    10861 agaagaaatt acgctgcttg cagtcagtaa aacaaaacct gcgagcgcca tcgcagaagc
    10921 cattgatgcc gggcagcgtc aatttggtga aaactacgtt caggaagggg tagataaaat
    10981 tcgccacttt caggaactgg gcgtaacagg attagaatgg cattttattg gcccgttgca
    11041 gtctaataaa agccgcctag tggcagagca tttcgactgg tgtcatacca tcgaccgttt
    11101 gcgcattgct acccgcctca acgagcagcg cccggcagaa cttccccctc ttaacgtact
    11161 gattcaaatt aatattagtg atgaaaacag taagtccggg attcaactgg cagaactgga
    11221 cgagctggca gcggcggtcg ctgaactacc gcgtttacgt ctgcgcgggc tgatggcaat
    11281 ccctgcgctt gagtcagaat atgtaaggca gtttgaagtt gcacgccaaa tggctgtagc
    11341 atttgccgga ctgaaaacgc gctacccgca tatcgacacg ctctctctgg gaatgtcgga
    11401 cgatatggaa gccgccattg cggcaggtag cacgatggtt cgtatcggca ctgcaatttt
    11461 tggtgcgcgt gattactcta aaaaataagg aattaaagga acgccatgaa tacgttgact
    11521 ttcctgcttt caacggtcat tgagctgtat acaatggtgc tgttattacg catctggatg
    11581 cagtgtgctc attgtgattt ttacaccccc ttctcacagt ttgtagtgaa ggtagcgcag
    11641 ccaattatcg ggccactgcg ccgcgttatt ccggcaatgg gtcccattga cagcgcctcg
    11701 ctgctggttg cctatattct cagttttatc aaagccattg tgctgtttga agtggtgacc
    11761 ttcctgccaa tcatctggat tgccggttta ctgattctgc tgaaaaccat cggcttgctg
    11821 attttctggg tcctgctggt gatggcgatt atgagctggg taagccaggg tcgtagcccg
    11881 attgaatacg tgctgattca gctggccgat ccgctgctgc gcccgattcg ccgcttgcta
    11941 ccggcaatgg gtgggattga tttctcgccg atgatcctcg ttctgctgct gtatgccatc
    12001 aatatgggtg tcgcagaagt attacaggca accggaaaca tgctgctgcc ggggctgtgg
    12061 atggcgttat gagtgccgta acagttaatg atgacggtct ggttttacgg ctctatattc
    12121 agccgaaagc cagccgtgat tctattgtcg gtttacatgg cgacgaagtt aaagtcgcca
    12181 ttaccgcgcc gccggttgac ggccaggcca acagtcatct ggtgaagttt ctcggtaagc
    12241 aattccgggt tgccaaaagc caggtggtga ttgaaaaagg cgaacttggc cgccacaaac
    12301 aaattaaaat cattaatccg caacaaatcc cgccagaaat cgcggcgtta attaattagg
    12361 tatcctatgc aaaaagttgt cctcgcaacc ggcaatgccg gtaaagtgcg tgagctggcg
    12421 tcgctgctta gcgactttgg tcttgatatc gtggcccaaa cagacctcgg cgttgattcc
    12481 gctgaagaaa ccggcctaac ctttatcgaa aacgcgattc tgaaagcgcg ccatgcggca
    12541 aaagtgaccg gtttaccggc aattgccgac gactctggtc tggcggtaga tgcgctgggt
    12601 ggcgcgccgg gtatttactc cgcgcgttat tccggtgaag acgcgaccga tcaaaagaat
    12661 ctgcaaaaac tgttggaaac actgaaagac gtaccggacg accaacgtca ggcgcgtttc
    12721 cactgcgtgc tggtatatct gcgtcacgcg gaagatccga ctccgctggt atgccacggc
    12781 agctggccgg gcgtgattac tcgcgaacca gcgggcactg gtggctttgg ttatgatcca
    12841 atcttcttcg taccttccga agggaaaacc gccgccgaac tgacccgcga agaaaagagc
    12901 gccatttccc accgtggtca ggcgttgaaa ctgctgctgg acgctttacg taatggttaa
    12961 attaccgccg ctgagtctct acattcacat cccgtggtgc gtgcagaaat gcccgtactg
    13021 cgatttcaac tctcacgcgt tgaaaggaga agtgccgcac gacgattatg ttcagcatct
    13081 gctttgcgat ctggacaatg atgtggctta cgctcagggc cgtgaagtaa agacaatctt
    13141 tattggcggt ggtacgccga gcctgctttc cggcccggcg atgcaaacgc tgctagacgg
    13201 cgtgcgtgcg cgtttgccgc tggcagcgga tgcagaaatt actatggaag cgaacccagg
    13261 cacggtagaa gcggatcgct ttgtcgatta tcagcgtgct ggtgtaaacc gcatctctat
    13321 tggtgtgcag agttttagcg aagaaaagct gaaacgactt gggcgtattc atggcccgca
    13381 agaagcgaaa cgagcggcaa agctggcgag cggtttaggg ttacgtagct ttaaccttga
    13441 tttgatgcat ggactaccgg atcaatcact ggaagaggcg cttggcgatc tacgccaggc
    13501 cattgaactg aatccgccgc atctttcctg gtatcaactg actatcgaac ctaatacgct
    13561 gtttggctcg cgaccgccgg tgctgccgga cgatgacgcg ctgtgggata ttttcgaaca
    13621 ggggcatcag ttattaaccg cagcgggtta tcagcaatat gaaacttccg cttacgccaa
    13681 acccggttat cagtgccagc acaatctcaa ctactggcgc tttggtgact acatcggtat
    13741 tggctgcggc gcgcacggca aagtgacctt cccggatggg cgcattctgc gtaccactaa
    13801 aacgcgtcat cctcgtggtt ttatgcaagg aaggtatctg gaaagccagc gtgatgtcga
    13861 agccgcagat aagccgtttg agttctttat gaatcgcttc cgtctgctgg aggccgcgcc
    13921 gcgcgtggag tttattgcgt ataccgggct ttgcgaagat gtgattcgcc cacagttaga
    13981 cgaggcgatt gcccagggtt atctcaccga atgtgcggat tactggcaga taacggaaca
    14041 tggtaaatta ttcttaaact cacttcttga attattcctc gctgagtaat cattaatgcg
    14101 cctgcgggcg cattaatcac ttcttttatc cccccgttaa ttaacctcgg cagaaatata
    14161 tttcttactg aataaatatc gacgacattt attatatata tttagtgaat atttactaat
    14221 cattcattga ttataaatat gtacaacctg gcgcacaaat ttaatattta atttctgact
    14281 ttatttcaag ttgtgataaa catagacaag ttgtcagaca gattaaccaa tttgtcgcac
    14341 aaatcactga taagcacaat tagatatcaa ccaatttcca tccttcaaca gggagttttt
    14401 ttatgaaaat gaggtcattt acccgctcat tagtctgcgc atccttgctg gctctggtta
    14461 gcactggcgt aaacgccgca gaaaaagtgg cgttaaagtt agcacacaac ctggaaagaa
    14521 gtcatgttgt acatcagagt tttgaagagc tggcgaaaga ggtaaaacag ctgtcaaaag
    14581 gtaatatggt gatccgtatt tatcccagca gccagatggg taatgcgcga gaaactatgg
    14641 aattgttgca aaatggcgca ctggatatga ccaaaggttc cgccagtgat ctggagtctt
    14701 ttgataatat ttatgctatc tataatttgc cgttcttatt aagttgtttt tggtgaagtt
    14761 ggtaaagaaa ttatggactc aacaaaagac aaaggctttt ttgctttatc agcttatgta
    14821 gcgggtactc gcagtttcta tgcgaaaaaa ccgattacaa agccagaaga tcttaaagga
    14881 ctgaaaattc gtgttcagcc aagtccgacc accatcaaaa tgattgaact gatgggtggt
    14941 tcacccactc caatttcatt tggcgaagtt tatactgcga tgcagcaagg tgtagtagat
    15001 ggcgcagaaa ataacgtgcc ttcctggatg cagactcgac atattgaaat tgccaaagtt
    15061 ttttctgaag atgaacacgc ctcaattcct gactttttgg ttatctccac caaaacatgg
    15121 aataaattaa ccccagaaca gcaacaaatc ctcgaaacag cggcgaagaa atccgaagct
    15181 tatcaacaaa aattgtggga aaaaattgac gctgacacac gtgctcaggc gaaagctatg
    15241 gggggtgaaa ttgtaaaagt ggataaagcg ccattccgta aagccgtgca acctctgttt
    15301 gacgacttca aaaaagatcc aaaacaagct gctttgttag agaaattcga caacgctgct
    15361 caataatatt ccgggccagc ctctggccct gattttccag gatgcttaca atgatattta
    15421 accgcctgaa acttgcggta gaccgcgtca ttgccacgtt ttctgtcgcc gtaatgcttg
    15481 ctctggttgt atgcgtagtc tggcaagtat ttagccgcta cgtacttaac cagccaagta
    15541 cattaaccga tgagctggca cgctttttga tgatctgggt aggattatta ggtgctgcat
    15601 ataccgtcgg cgcgcagcga catctctcta ttgatttatt tgctctcgcc ctgaataagc
    15661 gcaaacaatt acttcttagt atagtcatta acgttctcat tttaggtttt gccggttcgg
    15721 tcattgtgac tggtggcctg aaatta
//
LOCUS       DAJTYM010000087        15562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTYM010000087 DAJTYM010000000
VERSION     DAJTYM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            107..1165
                     /gene="citC"
                     /locus_tag="OO575_002953"
     CDS             107..1165
                     /gene="citC"
                     /locus_tag="OO575_002953"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6665839.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1180..1476
                     /gene="citD"
                     /locus_tag="OO575_002954"
     CDS             1180..1476
                     /gene="citD"
                     /locus_tag="OO575_002954"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6665840.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1473..2381
                     /gene="citE"
                     /locus_tag="OO575_002955"
     CDS             1473..2381
                     /gene="citE"
                     /locus_tag="OO575_002955"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6665841.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2392..3924
                     /gene="citF"
                     /locus_tag="OO575_002956"
     CDS             2392..3924
                     /gene="citF"
                     /locus_tag="OO575_002956"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6665842.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3928..4478
                     /gene="citX"
                     /locus_tag="OO575_002957"
                     /pseudo
     CDS             3928..4478
                     /gene="citX"
                     /locus_tag="OO575_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4453..5331
                     /gene="citG"
                     /locus_tag="OO575_002958"
     CDS             4453..5331
                     /gene="citG"
                     /locus_tag="OO575_002958"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6665843.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5382..6844
                     /gene="citT"
                     /locus_tag="OO575_002959"
                     /pseudo
     CDS             5382..6844
                     /gene="citT"
                     /locus_tag="OO575_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            6958..7764
                     /gene="rna"
                     /locus_tag="OO575_002960"
     CDS             6958..7764
                     /gene="rna"
                     /locus_tag="OO575_002960"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6665844.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            7993..8403
                     /gene="rnk"
                     /locus_tag="OO575_002961"
     CDS             7993..8403
                     /gene="rnk"
                     /locus_tag="OO575_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6665845.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8634..9843)
                     /locus_tag="OO575_002962"
                     /pseudo
     CDS             complement(8634..9843)
                     /locus_tag="OO575_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            10064..10492
                     /gene="uspG"
                     /locus_tag="OO575_002963"
     CDS             10064..10492
                     /gene="uspG"
                     /locus_tag="OO575_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6665846.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10613..12178)
                     /gene="ahpF"
                     /locus_tag="OO575_002964"
     CDS             complement(10613..12178)
                     /gene="ahpF"
                     /locus_tag="OO575_002964"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6665847.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12423..12986)
                     /gene="ahpC"
                     /locus_tag="OO575_002965"
     CDS             complement(12423..12986)
                     /gene="ahpC"
                     /locus_tag="OO575_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6665848.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13358..14104
                     /gene="dsbG"
                     /locus_tag="OO575_002966"
     CDS             13358..14104
                     /gene="dsbG"
                     /locus_tag="OO575_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6665849.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14314..15216
                     /locus_tag="OO575_002967"
     CDS             14314..15216
                     /locus_tag="OO575_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6665850.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15363..15562
                     /locus_tag="OO575_002968"
                     /pseudo
     CDS             15363..15562
                     /locus_tag="OO575_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3818 a         4048 c         3797 g         3899 t
ORIGIN      
        1 tcacccccct ttctcccaag tgaaaataaa aggttatcag tttgcaacat tgaacaacat
       61 tcgttgcaaa tcgataacaa catgcacctt caggatacta tttattatgt tcggcaatga
      121 tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct
      181 gcatgaaaat gatttgagcg ttgacaccac agtcgaagta tttattaccg taacccgcga
      241 tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt attaaatgcg ttgctatcag
      301 tgaatccgtt cgcggtgaag gactggcgct gacattagcc actgaactga taaacctcgc
      361 ctatgagcgg cacagcacgc atctgtttat ttataccaaa accgaatacg aggcgctgtt
      421 ccgccagtgc ggtttttcca cgctgacctg cgtacccggc gtgatggtgc tgatggaaaa
      481 cagcgccacg cgactgaaac gctatgccga atcgctgaaa aaatttcgtc atccagggaa
      541 caagattggc tgcattgtga tgaacgccaa tccctttacg aatggtcacc gttatctgat
      601 tcaacaagct gcggcacagt gcgactggtt gcatctgttt ttagtcaaag aagattcttc
      661 acgcttcccc tatgaagacc ggctggatct ggtgttaaaa ggcaccgccg atattccacg
      721 cctgactgtg catcgcggct cagaatacat catctcccgc gctacgttcc cttgctactt
      781 cattaaagaa cagagcgtca ttaaccattg ttacaccgaa attgatctga agattttccg
      841 tcagtacctc gctcccgcgc ttggtgtaac tcaccgcttt gtcggtactg aacccttttg
      901 tcgcgttacc gcccagtaca accaggatat gcgctactgg ctggaaacgc cgactatctc
      961 cgcagcgccc atcgaactgg ttgaaattga gcggctgcgt taccaggaga tgccgatatc
     1021 cgcttcccgg gtacgtcaac tgctggcgaa aaacgatctc acggctatcg cgccgctggt
     1081 ccctgcagtc acgctgcatt atttgcagaa cctgcttgag cactcccgcc aggacgcggc
     1141 agctcgtcaa aagacccccg catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc
     1201 gttgcaggca cccttgagtc tggggatgtg atgatacgca tcgccccact cgatacgcag
     1261 gatatcgacc tgcaaatcaa tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc
     1321 accattctgg acgttctcgc ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac
     1381 aaaggcgcac tggactgcat tttacatgca cgactggaag ccctgctggc acgcgccagc
     1441 ggtatcccgg ctctgccatg ggaggattgc caatgatttc cgcttcgctg caacaacgta
     1501 aaactcgcac ccgccgcagc atgttgtttg tgcctggtgc caatgccgcg atggtcagca
     1561 actccttcat ctacccggct gatgccctga tgtttgacct cgaagactcc gtggcattac
     1621 gtgaaaaaga caccgcccgc cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg
     1681 atattgaaac cattgtgcgt gtcaacgcgc tggattccga atggggtgtt aacgacctgg
     1741 aagccgtcgt tcgcggtggt gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg
     1801 atgttctgga tattgaaaaa gagatcctgc gtatagaaaa agcctgtggt cgtgaacccg
     1861 gcagcaccgg cctgctggcg gcgattgaat ctccgctggg cattacccgc gcagtggaaa
     1921 tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca
     1981 acctgcgtac agaacgctcc ccggaaggaa ctgaactgct gttcgcacgc tgttccattt
     2041 tgcaggccgc gcgctctgcg ggtattcagg cgttcgatac cgtctattcc gacgctaaca
     2101 acgaagccgg attcctgcaa gaagccgccc acatcaaaca gctgggcttt gacggcaaat
     2161 cgctgatcaa cccgcgtcag attgatctgc tgcacaacct ctacgcaccg acccagaaag
     2221 aagtggatca cgcccaccgc gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg
     2281 gcgtggtttc cctgaacggc aagatggtgg acggtccggt tatcgatcgc gcccgtctgg
     2341 tgctctcccg tgcagaactt tccggcatcc gcgaagaata aggcaatcaa aatgacgcag
     2401 aaaattgaac aatctcaacg acaagaacgg gtagcggcct ggaatcgtcg cactgaatgc
     2461 gatcttgccg ctttccagaa ctcaccaaag caaacctacc aggctgaaaa agcgcgcgat
     2521 cgcaaactgt gcgccaacct ggaagaagcg attcgtcgct caggtttaca ggacggcatg
     2581 acggtttcct tccatcacgc tttccgtggc ggtgacctga ccgtcaatat ggtgatggac
     2641 gtcatcgcga agatgggctt taaaaacctg accctggcgt ccagctccct gagtgattgc
     2701 catgcgccgc tggtagaaca cattcgccag ggcgtggtta cccgcattta tacctctggc
     2761 ctacgcggtc cactggcgga agagatctcc cgtggtctgc tggcggaacc ggtgcagatc
     2821 cactctcacg gcggtcgtgt gcatctggta cagagcggcg aactgaatat cgacgtggct
     2881 ttcctcggcg tcccgtcctg tgatgaattc ggtaatgcca acggctacac cggtaaagcc
     2941 tgctgcggct ccctcggcta tgcaatggtt gatgccgaca acgcaaaaca ggtcgtgatg
     3001 cttaccgaag aactgctgcc ttatccgcat aatccggcaa gcattgagca agatcaggtt
     3061 gatttgatcg tcaaagttaa tcgcgttggc gatgctgcaa aaatcggcgc tggtactacc
     3121 cgtatgacca ctaacccgcg cgaactgctt attgcccgta gcgctgcgga tgtgattgtc
     3181 aactctggct acttcaaaga aggtttctcc atgcaaaccg gcaccggcgg cgcttcgctg
     3241 gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc
     3301 ctcggcggta ttaccgcgac gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg
     3361 ctggatgtgc agagctttga cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac
     3421 atcgaaatca gcgccaacca gtacgctaac tggggttcga aaggcgcatc ggttgatcgt
     3481 ctcgacgtgg tggtactgag cgcgctggaa attgacaggc agttcaacgt taacgtgctg
     3541 accggttctg acggcgtact gcgtggcgct tccggtggtc actgcgatac cgcgattgcc
     3601 tctgcgcttt ccatcatcgt cgcgccactg gtacgcggtc gtattccgac tctggtggat
     3661 aacgtgctga cctgcatcac cccgggctcc agtgtcgata ttctggtcac agaccacggt
     3721 atcgcagtta acccggcacg tccggaacta gcagaacgtc tgcaggaagc gggcattaaa
     3781 gtggtttcca ttgagtggct gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg
     3841 attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat
     3901 gttgtgcatc aggtgaagga ataagccatg cacctgcttc ctgaactcgc cagccaccat
     3961 gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgcc
     4021 tggctcaagc accatcctgt tccactggtc tcctttaccg tggttgcgcc tgggccgatt
     4081 aaagacagcg aggtcacacg ccgaattttt aatcatcgcg tgacagcctt gcgtgcctta
     4141 gccacaaagc agggctggca aattcaggag caggctgcac tggtttccgc cagcgggccg
     4201 gagggcatgt tgagcattgc cgccccggct cgcgacctca agctcgccac cattgagctt
     4261 gaacatagtc atcctctcgg gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa
     4321 attctctccc gccgcgacta ttcactgcgc ctcgccgctg cctgttgtgc gagcaaagcg
     4381 cagccgtctg cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg
     4441 cactgctgaa cgatgtcgat gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca
     4501 ttaatcgatg agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca
     4561 ccgaaaccag gcctcgtgga tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa
     4621 gatttccacc gcagcgcgct ggcgattcag ggctggttac cccgtttcat tgaatttggt
     4681 gcctgtagtg cagaaatggc accggaagcg gtactccacg gattacgccc aattggtatg
     4741 gcttgcgaag gtgatatgtt ccgcgccact gcgggcgtaa acacgcataa aggcagcatt
     4801 ttttctttag ggctgctatg tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt
     4861 acgccaacaa ccgtttgttc tacggcggca agtttctgcc gtggcctgac cgatcgcgaa
     4921 ctgcgtacca ataattcacg actgacggca ggtcaacggt tgtaccagca gcttggcctt
     4981 accggcgcac gcggtgaagc cgaagcaggt tatccactgg tgatcaacca cgccttgccg
     5041 cattacctca ctctgctgga tcaggggtta gatcctgaac tggcattgct cgataccttg
     5101 ctcctactga tggctaccaa cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc
     5161 ctgcgctggc tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc
     5221 accgatctcg attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca
     5281 ggcggcagtg ccgacctgct gatccttacc tgttttttag cacagattta attatttaag
     5341 cacttgataa atttggaaat attaattttc ggagaacccg tatgtcttta gcaaaagata
     5401 atatatggaa actattggcc ccactggtgg tgatgggtgt catgtttctt atccctgtcc
     5461 ccgacggtat gccgccgcag gcatggcatt acttcgctgt gtttgtggca atgattgtcg
     5521 gcatgatcct cgagccaatt ccggcaacag cgatcagttt tattgcggtt actatttgcg
     5581 ttattggcag taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg
     5641 cgcaaaaaca ggcgctgaaa tggggcctgg ctggtttttc cagcaccacg gtatggctgg
     5701 tatttggcgc atttattttt gcattagggt atgaagtttc cgggttaggt cgtcgcattg
     5761 cccttttcct ggtgaaattc atggacaaac gcacgctgac gttgggttat gcgattgtca
     5821 ttatcgacat tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg
     5881 tttttccggt cattaaaaac ctgccgccgt tgtttaaatc attcccgaac gatccgtccg
     5941 cgcgtcgtat tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt
     6001 ccatgtttgt caccggtgcg gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc
     6061 cggtatccag attagctggt tgcagtggtt cctctgcttc ctgccggttg gggttatctt
     6121 gcttatcatt gcgccgtggc tttcctacgt gctgtacaaa ccggaaatca cacacagtga
     6181 agaagtggca acctgggcgg gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga
     6241 gtggacgctg attggccttg tattgctcag cttaggtttg tgggtatttg gcagtgaagt
     6301 cattaatgct actgcggttg gtctgctggc agtttcgcta atgctggctc tgcacgttgt
     6361 gccgtggaaa gacattaccc gctataacag cgcatggaac acgctggtca acctggcaac
     6421 tctggttgtg atggctaacg gcctgactcg ttctggtttt attgactggt tcgccggtac
     6481 catgagtacg cacctggaag gattctcacc aaacgcaacg gtgattgtac tggttctggt
     6541 gttctacttt gcacactacc tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc
     6601 ggttattctg gccgtcggta aaggtattcc gggcgtacca atggaacaac tgtgtatcct
     6661 gctggtgctg tctatcggta tcatgggctg tctgacgccg tatgcaaccg gtcctggggt
     6721 gattatttac ggctgtggct atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt
     6781 cggggtgatt tacatctcta tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa
     6841 ctaatacgtt cgccaaacgc tgaataaatc atcacgcccg ccaggtaagc ccacctggcg
     6901 ggctttttta tgatttaata gatagtattc agtgtatgta tgagttccac acccattatg
     6961 aaagcattct gccgtaacgc cgcgttgctc gcggtttctc tgcttccctt atcttctgcc
     7021 aacgccgtag cgttgcaggc aaaacagtat ggcgattttg atcgctatgt cctggccctc
     7081 tcctggcaaa ccggattttg ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa
     7141 tgtcgcctgc aaaccgaaac gaccaacaaa actgattttc tgaccgtaca tggtctgtgg
     7201 ccaggattgc ctaaatcggt tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc
     7261 ggttgcgcta ctcgcccaat cccgaatcta ccagaagcac gcgccagccg aatgtgttca
     7321 tcgccggaaa ccgggttatc actggaaacg gccgctaaac taagtgaagt catgccagga
     7381 gctggcggac gttcctgcct ggaacgctac gaatatgcca aacacggtgc ctgctttggt
     7441 tttgatccgg acgcatactt cggtacgatg gtacgcctga atcaagaaat taaagagagc
     7501 gaagccggaa aattccttgc ggataattac ggtaaaacag tgagccgccg tgactttgac
     7561 gccgcctttg ccaaaagctg gggaaaagag aacgtgaaag cagttaagct aacgtgccag
     7621 ggtaaccctg cgtatttgac tgaaattcag atctcgatca aagctgacgc catcaacgct
     7681 ccgctttctg caaactcatt cttgccacaa cctcacccag gtaactgtgg caaaaccttt
     7741 gtgattgata aagcgggtta ttaatcctct gatttacaaa acacgctaat tcgtcttcca
     7801 taaccatgcc gattaagaat tatacataat gcgctgcatt gaggaattcc ctgaatgtga
     7861 cgcaaatcac ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc
     7921 gcctgacggt gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga
     7981 catggagtaa aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc
     8041 gatattctgc tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca
     8101 gagttggatc gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg
     8161 aacagccggg ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg
     8221 tatccggcaa aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc
     8281 gcactgctgg ggctgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca
     8341 acccaccttg aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt
     8401 taatcccatc tgcacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt
     8461 acgcaaaatc agcaattttt cctggttctc actgttcact tttgctgcaa aattattccg
     8521 tttcgtttgt gggaataacc atggcagaag cattttacat cctgatagga tttctgatta
     8581 tggcggcgat tatcgtcatg gctgtccttt acctcgaaaa ccactcctga cgatcatatt
     8641 gttccccccg gcatcgcatt caccagacct gaaaccgcct tctgcgccgc ctctgcgctt
     8701 tgtgcaccgg gtaccagaat caccttacgg cactcctctt cacgtttttc gaaaatctca
     8761 tatccccggg cggcctcttc aaacggcata tagtgggtaa caatttcttc tggtttcagt
     8821 aatcctttct caattaacgg taataattct cccagccatg cgtgaacgtg ggtctgtccc
     8881 attttaaacg tcaacccttt atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca
     8941 gcgtagacgc ccggtacgct aacaatgccg caacgcttta ccgctgctgc cctccagttt
     9001 caggttagtc agcaccgttt ccgtggtgct gcctttcgct tcaaaaccga cggcgtctat
     9061 tactgcatcc acgccccggt gacctgccgt ttgttcaata attgactgtg ccggatcgct
     9121 atcttcatcg aaattaatcg ggatcgcgcc gtagcggtca gcggcgaaac gcaagcggta
     9181 gggatgatga tcaacaacaa aaatctgttc cgcaccgagc aaccgtgcac aggcgattgt
     9241 caacaatccc acaggaccag caccatagac tgcaacgctt gaaccttgtt ggatctgcac
     9301 attttttgct gcctgccatg ccgttggcag aatatcagaa aggaaaaggg ctttatcatc
     9361 tgaaagcaaa ggcggtactt taaacggccc cacattccct ttcgggacgc ggacatattc
     9421 cgcctgcccg ccaggaacgc caccatacag gtgactataa ccaaacaatg ccgcgggagc
     9481 aggtatctgt tttttattga gcgcagcgcc tttacccgca ttggtatttt cgcaggcggc
     9541 atattgctgt aatcgacaga aaaaacagtc gccacaagca atgacgaacg gaattaccac
     9601 ccggtcgcct ttttgcaaat tttttacgtc cttcccggtt tcaacgactt cccccataaa
     9661 ttcatgacca aaaatatcgc catgtttaac ctggggtatt ttgcctcgat aaagatggag
     9721 gtcagagcca cagatcgccg ttaccgtaat acgcagaata atatcatctg cctgttcaat
     9781 gcccggatcg ggaacatttt ctacctgaac gtgatgtggg ccgtgataag tcaatgcttt
     9841 catgcaacct ccagagacag ttcaaaaaag agtcagtctt taagggtagc tatcacagcg
     9901 ggaaaacggg cggtaccgca ggttttggga gaattcctag tgttcagtga gaatatatta
     9961 atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat atcagggata aatattataa
    10021 tcatggagaa tgatttgttc atgattaaca gggagaaagg gttatgtata aggcaatcat
    10081 tatgccagtt gatgtatttg aaatggaatt gagcgacaaa gctgttcgcc acgctgaatt
    10141 cctcgcccag gatgacggag ttattcatct acttcacgta ctacccgggt cagccagcct
    10201 gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa gagcatctgc aacatgaagc
    10261 agaagaacgt ctgcaaacga tggtcagcca cttcaccatc gatccttccc gcattaaaca
    10321 acatgtccgt tttggtagcg tgcgggatga agtcaatgag ttggcagaag aactgggggc
    10381 tgatgttgta gttatcggtt ctcgcaaccc atcgatttcg acccatctgt taggttctaa
    10441 cgcctcgagc gtgatccgcc acgccaatct gcctgtactg gtcgttcgct aacagataaa
    10501 agtatttatg ccgccctgcc tggaacatcc cggcagggct ttttaatgcc ataaaaaagc
    10561 cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa tcttacttct tcttatgcag
    10621 ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc tttggcacct tcgccagtgg
    10681 cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc cgctgcgaac acgcctttga
    10741 cgttggtttc acatttcgca tcaatgataa tctcgcccat gcggttacgt tcgactgcgc
    10801 cttccagcca gttggtgttc ggcagcagac caatctggac aaaaataccg gccagttcga
    10861 tgttgtgaat attgccgctg acacgatctc gatattccag accaacgact ttgctgccgt
    10921 cgcctttcac ttccgtggtt tgcgcattca gaataatgtc gacgtttttc aggctgcgca
    10981 gtttgtcctg cagaacctgg tcggctttca tttctggcgc aaattccagc agcgttacgt
    11041 gctcaacgat acccgccagg tcgattgccg cttccacgcc ggagttaccg ccgccgataa
    11101 ccgctacgcg tttgccttta aacagcgggc cgtcgcagtg cgggcagtag gtcacgcctt
    11161 tagtgcgata ctgatcttcg cccggaacgt tcatgttgcg ccattttgca cccgtcgcca
    11221 cgataatgct gcgtgctttc agtaccgcgc cagaagctgt ttcaatctgg tgtagaccac
    11281 cttcaaccgc tgccgggatc agtttgctgg cgctctggct gtcgatcaca tcaacgtcgt
    11341 attcatcaac gtgaactttc agtgcgcctg ccaacttctg cccttcagtc ttcggtacag
    11401 aaatgtagtt ttcgatatca acggtatcga ggatctgacc accaaaacgt tcgcccatca
    11461 gaccggtacg gatgccttta cgtgcggagt aaattgctgc cgctgcaccc gccgggccgg
    11521 aaccaacgat taatacgtca taagcatcac gcttgttcag ctcttctgcc gcacgatttt
    11581 ccgcgccagt atcaattttg gcaacgattt cagtcaacgt catgcggccc tgaccaaact
    11641 ctttcccgtt tacgaacact gccggaacgc ccatcacgtt gcgatcggtg atttcgttct
    11701 ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg gttcaatacg ctcatcaggt
    11761 tcagcgcctg caccacgtcc gggcagttat ggcaagagag cgagtaatag gtttcgaatt
    11821 caaaatcacc gtcaatatgg cgaatctgct ccagcagaga ctgcgcttct ttcgacggat
    11881 gaccaccggt ccacagcaac gccagtacca gcgaggtgaa ctcgtggccc agcggggagc
    11941 ctgcaaaacg tggtccctgg ttggaacctg ggttggtgat caggaaagac ggcttacgca
    12001 ctggcaagct gttatcttct ttaaaggtga ctttgtctga cagttctgcg atttcagcca
    12061 acagttcctt gatttctgcc gatttagcgc tgtcatccag cgtggcaatt aactcaacag
    12121 gcttggtcaa tttctcaagg taagccttga gttgagtttt catatttgtg tcgagcatgt
    12181 ttatctcctg ggctttaaac atcatcatgc aagcggcctc tgcggctacc tggatgcagc
    12241 ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca cccgaagaat taggtgaatt
    12301 tccgaagtat ttcgcgtcag agcaaggcga ctagcctgtg aatccccggg agcttacaca
    12361 agtaagtgac cggggtgagc gggcgacgcc aacgccgcta tggcgtgaaa gacgaaggaa
    12421 atttagattt taccaaccag gtccagagac ggagccagag ttgcttcacc ttctttccat
    12481 ttagccgggc aaacttcacc tgggtgagaa gctacgtact gagctgcttt gattttacgc
    12541 agcagatcag acgcgtcacg gccaatgcct tcagcggtaa cttcgattgc ctggatgata
    12601 ccctgcgggt caacaacgaa ggtcgcacgg tcagccagac cttcatcttc acgcatgttg
    12661 tcgaagttac gggtcagggc gccagtcggg tcgccgatca tcgcatattt gattttagcg
    12721 atggtttcag agctgctgtg ccatgctttg tgggtgaagt gagtatcggt agatactgcg
    12781 tatacgtcta cgcccagttt ctgcagttct tcgtagtggt cagcaacgtc acccagttcg
    12841 gtcgggcata cgaaagtaaa gtcagccggg tagaagaaga agacgctcca gcggccttcg
    12901 gtatcttttt cggtgatttc gatgaattcg ccgtttttga atgcctggtt tttaaaaggt
    12961 ttaattttgg tgttaatcaa ggacatctat acttcctccg tgttttcgat gagatgtaag
    13021 gtaaccaatt tttgccgatt cggctaatgc gtttccatta tcaaatcgat aagttttacc
    13081 ttacaacctt cgtaagacaa ctttgtgaac tttgcaccgc cagcggcgat aaaaagtaaa
    13141 atggccccct ttccaggtga ccgtgtaatc tgaactaccc tttggtatct tcagcgcctg
    13201 cctgcaggct gaggtgttgc ccttacaaat gcaacaacga catggattac aacaccctca
    13261 taaacaaagg gcaatcacct gatctaagct cttacctatg acagtgatag gttatgcctt
    13321 ttactcgact tttgcactga ctgaaaagaa caaattaatg ttaaaaaaga tacttttact
    13381 ggctctgctt cctgcaatcg ccttcgcaga ggaacttcct tccccagtaa aagcgattga
    13441 aaaacagggc attacaatca tcaaaacgtt cgatgccccc ggaggaatga aaggttatct
    13501 cggaaagtat caggatatgg gcgtcaccat ctacctgact cccgatggta agcacgctat
    13561 ctctggttac atgtacaacg agaaaggtga aaacctgagt aacacactta tcgaaaaaga
    13621 aatttacgca ccagccggac gcgaaatgtg gcaacggatg gaacaatccc actggctcct
    13681 cgacggtaaa aaagatgcgc cggtcattgt ctacgtcttc tccgatccgt tctgcccata
    13741 ttgtaaacag ttctggcagc aggcgcgccc gtgggtagat tctggcaaag tgcaattaag
    13801 aacattgttg gttggggtta tcaagccaga aagcccggcg acagcagcgg caattcttgc
    13861 ctccaaagat cccgcaaaaa cctggcaaca atatgaagcc tctggttgca agcttaagct
    13921 aaacgtgcct gcaaacgtaa gtacagagca aatgaaagtg ttaagtgaca atgagaaact
    13981 gatggacgat ctgggggcaa atgtcacgcc ggctatctat tacatgagta aggaaaatac
    14041 gctgcaacag gccgtggggt tgcccgatca gaaaacgctt aatatcatta tgggaaataa
    14101 ataaaaggta attcatggat ggttgaacta tatccctgct tttcaaccat cctaaaaaag
    14161 catgaatttc tcgctctacc ctaagtggct gtgctatttt agaaaaactt aatcctgagt
    14221 taagcaaaaa aacataatca ataaaaatac atacgcaaaa aacattgatt aagtgaatat
    14281 accatggaag aaaaatataa accggagtag tgtatggcca atctctacga cttgaaaaag
    14341 ttcgacttaa atcttcttgt catatttgag tgtatttacc agcatttaag cattagcaaa
    14401 gctgctgaat cactatatat caccccatca gccgtgagcc agtcgttaca acgcttacgt
    14461 gcgcaattta atgacccttt attcattcgc tcaggaaaag gtatcgcgcc gacgacaaca
    14521 gggttgaatc tgcatcacca tcttgagaaa aacctcaaag gccttgaaca aacaattaat
    14581 atagtcaata agtccgaact aaagaaaaac tttattatct acggccctca gctcatttct
    14641 tgctctaata atagtatgct gatccgctgc ctacggcaag atgcctctgt cgagattgag
    14701 tgccatgata tcctcatgtc agcagagaat gctgaagaac tgttggttca ccgcaaagcc
    14761 gatctggtga tcacccaaat gccagtgatc agccgttccg tcatttgcat gccattgcat
    14821 acaattcgca atactctaat ttgtagtaat aagcatccgc gaattactga caatagcact
    14881 tatgaacaaa ttatggcaga agagttcacc caattaatag ccaaatcagc aggagttgat
    14941 gatattcaaa tggaaataga cgagaagttt atgaatcgca aaatttcatt tcgtggttcc
    15001 tctttgctga caataattaa tagcattgca gttacagatt tactgggaat agtcccctat
    15061 gaactttata actaccaccg tgactttctt aatttaaaag agataaaact agaacatccg
    15121 ttaccttcaa taaagcttta catttcctat aataaatctt ctttaaataa tttggtgttc
    15181 tcccgattta ttgaccgtct aaatgagagt ttttgatata taactataga cagctactat
    15241 aactttcatc tatttattca cagcgcgaat gaaaaataaa acactctgct ttacagtgtt
    15301 tactttataa ttaacagtct tatctcactg ttcaccctgt aacgttcaga gagcgtcttc
    15361 agatgtctat ttataaaatt cctcttccgc tcaatattct ggaagctgca cgagagcgta
    15421 ttacctagac actcaacacc ctaccccgcg tttgtgtctc attttctggc ggcaaagact
    15481 ctggtttaat gctgcatctg acagccgaac ttgcccgaca aatgggcaaa aaaatctgcg
    15541 ttttgtttat caactgggag gc
//
LOCUS       DAJTYM010000088        15526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTYM010000088 DAJTYM010000000
VERSION     DAJTYM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO575_002969"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO575_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6665851.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO575_002970"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO575_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6665852.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO575_002971"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO575_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO575_002972"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO575_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6665853.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO575_002973"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO575_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6665854.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO575_002974"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO575_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6665855.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO575_002975"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO575_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6665856.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO575_002976"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO575_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6665857.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO575_002977"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO575_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6665858.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO575_002978"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO575_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6665859.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO575_002979"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO575_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6665860.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO575_002980"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO575_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6665861.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO575_002981"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO575_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6665862.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO575_002982"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO575_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6665863.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO575_002983"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO575_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6665864.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO575_002984"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO575_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6665865.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO575_002985"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO575_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6665866.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO575_002986"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO575_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6665867.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO575_002987"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO575_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6665868.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO575_002988"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO575_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6665869.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO575_002989"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO575_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6665870.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO575_002990"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO575_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6665871.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO575_002991"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO575_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6665872.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO575_002992"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO575_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6665873.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO575_002993"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO575_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6665874.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO575_002994"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO575_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6665875.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO575_002995"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO575_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6665876.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO575_002996"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO575_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6665877.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO575_002997"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO575_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6665878.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO575_002998"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO575_002998"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6665879.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OO575_002999"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OO575_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6665880.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3643 c         4130 g         3746 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAJTYM010000089        15051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTYM010000089 DAJTYM010000000
VERSION     DAJTYM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..275)
                     /locus_tag="OO575_003000"
     CDS             complement(<1..275)
                     /locus_tag="OO575_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665881.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVT"
     gene            complement(728..1990)
                     /locus_tag="OO575_003001"
     CDS             complement(728..1990)
                     /locus_tag="OO575_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6665882.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(2188..2263)
                     /locus_tag="OO575_003002"
     tRNA            complement(2188..2263)
                     /locus_tag="OO575_003002"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2228..2230),aa:Phe,seq:gaa)
     gene            complement(2369..3076)
                     /locus_tag="OO575_003003"
     CDS             complement(2369..3076)
                     /locus_tag="OO575_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6665883.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3474..5609
                     /gene="speC"
                     /locus_tag="OO575_003004"
     CDS             3474..5609
                     /gene="speC"
                     /locus_tag="OO575_003004"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6665884.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5658..6914)
                     /gene="nupG"
                     /locus_tag="OO575_003005"
     CDS             complement(5658..6914)
                     /gene="nupG"
                     /locus_tag="OO575_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6665885.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7116..8195)
                     /gene="mltC"
                     /locus_tag="OO575_003006"
     CDS             complement(7116..8195)
                     /gene="mltC"
                     /locus_tag="OO575_003006"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6665886.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(8260..8535)
                     /locus_tag="OO575_003007"
     CDS             complement(8260..8535)
                     /locus_tag="OO575_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6665887.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8563..9615)
                     /gene="mutY"
                     /locus_tag="OO575_003008"
     CDS             complement(8563..9615)
                     /gene="mutY"
                     /locus_tag="OO575_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6665888.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9776..10495
                     /gene="trmB"
                     /locus_tag="OO575_003009"
     CDS             9776..10495
                     /gene="trmB"
                     /locus_tag="OO575_003009"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6665889.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10495..10821
                     /locus_tag="OO575_003010"
     CDS             10495..10821
                     /locus_tag="OO575_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6665890.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            11005..11724
                     /locus_tag="OO575_003011"
     CDS             11005..11724
                     /locus_tag="OO575_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6665891.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11900..12946
                     /gene="ansB"
                     /locus_tag="OO575_003012"
     CDS             11900..12946
                     /gene="ansB"
                     /locus_tag="OO575_003012"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6665892.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            13063..14025
                     /locus_tag="OO575_003013"
     CDS             13063..14025
                     /locus_tag="OO575_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6665893.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(14126..>15051)
                     /locus_tag="OO575_003014"
     CDS             complement(14126..>15051)
                     /locus_tag="OO575_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR6665894.1"
                     /translation="LNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVLIVFSLTAGG
                     VSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAFLDALPSLLL
                     VFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTTSIVLL
                     LIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLI
                     FTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPL
                     LLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3922 a         3614 c         3849 g         3666 t
ORIGIN      
        1 gtaacaatat caggcgcgcc tgcggaacgg tccttaccat gctgcagtaa ccggaaataa
       61 acttttgggc gcaagatgac atcgaccgtt tcgacatcgt ctttttctcc ctcaaaaaat
      121 ttactgacaa tcgcttcgtc aagacgtccc gccgctatcc catgccagtg agtgaaattt
      181 tgggcgtctt tccgtttaaa actgcccttt cctgactcag catccgcaag cgagttgcgc
      241 cagtatgagg caaaccctaa agcattttca tccattacca gtcctgtgac tcaatgaaat
      301 ccattgattt atcgaatata aaatcaccgt atggacagct attgtccata ttgaaaaagt
      361 tggatgcaat gttttgttta taattcgata tcaaatgaac tggttgtaca aggttcagaa
      421 cacaagctca ttaaacagta cgtcaggact attgatttca taacagtgaa tacatttttt
      481 accgtttata ataactacct gtgttggacg gggataccag agcagccgaa aacaccgggg
      541 gttattacct gctctcatgg aaaccatata ttcctggtgc taatcacagt cataaatagc
      601 ctgaagaacg tgacgaaggg actttaatgg aagccggagt aacttgtcag aaacatcaat
      661 cttcgtcatc atttacaaag aaaaataatt agttaaagca gtccgggctg attgcccgga
      721 ctgcttacta ttttaatgga ttgttaatct ttgcatattc aaacgggctg atacccttct
      781 cccggttcgc atctagaaaa tcggcccacc attgcaacat cagtcgccgt tcttccagat
      841 gttctgcttt atggatatac gcagcacgta caccattacg ctcctgatgg ctcatctgac
      901 gttccacagc atcgcgggac cacaaacctg attcaatcaa tgcactgcac gccatcgccc
      961 ggaatccatg gccacagact tcctgggttg tgtcataccc catcaccctc agggctttat
     1021 ttacagtatt ttcactgatt ggtttacgcg ggtcatgtgc acccgtaaag atcagaccat
     1081 tttcaccagc ccatgtctga agttctgcca gtatggctac aacctgtttg ctgagtggca
     1141 caagatgtgg tgttcgcatc ttggatcccc ggtgtgagaa ttttacgcct ggcagaggtt
     1201 cgcgttcggc tggaatagtc cacatggaat tgtcaatatc aatttccgac cagcgggcat
     1261 atctgagttc actggaacga ataaaaacca gtaaattcag cataactgcc agtcgggtga
     1321 caggctggcc tttataaccg tcaatacgcg acagaagttc aggcaggcgt gaaagctcca
     1381 gagcagggcg atgctggcgt tttaccgtgg tcaatgcacc agccatatcc agagccgggt
     1441 tatatctgat gatgccagac tgtacggcgt aacgcattac agcagatatt ctttgctgca
     1501 gacgactggc gatttcataa atttgttttg cttctgcagc acgaacaggg ataagcaggt
     1561 ccggggtatt gagtgttgtg atatcccggt tgccgataaa ggggaaaacg tgtgtctcga
     1621 ggcttttgag tactttatcg ctgtgtgatt gtgcccattg tttattactg gcatgccagc
     1681 gtcgtgcaat atgttcaaaa gagagctgta aatcagggac aactttttta actcttttct
     1741 gttcgcaagg gtcaatcccc tctgaaatca gttttcgggc ctcatcccgt tttgtacgag
     1801 cttcggagag agaaacttct ggatacacac caagtgccag agtcttctcc ttacccagaa
     1861 tacgataacg gagacgccag taacgggaac cattaggatg aaccagcagg aacatgccgg
     1921 ccccgtcagt gagtttataa gccttgtcag tgggctttgc agcccggatt tttgcgtcag
     1981 tcagtgccat agttcagcct tctgaatgcg ttaaagaggg tacaaattct gtcgaactgg
     2041 ggtatacccg caattatacc cacacaatgg ggttgatgtg gggggaatct ggttgagttc
     2101 ggtagaattg atttggagga agaacactta aatcatggtg ttctgaaacg aaaaacggac
     2161 ctccgtggag gtccgttgat atgaatttgg tgcccggact cggaatcgaa ccaaggacac
     2221 ggggattttc aatcccctgc tctaccgact gagctatccg ggcaacgggg cgcattaaac
     2281 ctgattcgcc tcgtctcgtc aatcaaattt cttcaattta ctgcagactg cacaatctat
     2341 catcactttg ccgctattgc acgcattgtc aggcaaacca ggcggtccag gcggcggaaa
     2401 gcggcattgc cagaaggagt gctggaagca tgttgaccac cgggaacatt ttaatgccac
     2461 agatgcgtaa tccggttgcc agcagcaata aaccgcctac ggcgctgaag tctgccatca
     2521 tcgacggtgt gatcagcggt aatatcagcg cggcagccca cgccagcgtt aactggatga
     2581 tcagtaatgg gatactaatt accgacaccg caatacccag cgagcaggcg aagatcatcg
     2641 ccgtaaagaa atcaagaaat gacttggcga ttaaaatact cggatcgccg gtcatccctt
     2701 cgttcatcgc cccgaagatc ccggtgccgc tggcgcaaaa caggacaata atcgcgacat
     2761 aattctgaat aaaagattca tgcgctggct tcttacgtga gtgacgaaac agattttgtg
     2821 ctttggcgac tgctgtattg acacctttct ccagcaaaca aatttcgcca attagagcgc
     2881 caagtagcgt agctaaaacc atcgctggaa ggttggcgca tttcacaacc agtaaaatac
     2941 caatccccag cgatgccaga ccaaaaatag atgtcatgga gacgcggata cgttccggta
     3001 agcgttggct gagcagtgcg ccgagaacgc cacccagtaa gactgcacta gcgttgataa
     3061 aagggccgat gaccacaaga gttcctgtta gctgtattgt ttgattttct tattatgaac
     3121 tttttgcctt gttgatggct tgtctggtcg caaagtcctg gcttgcacgc cttagcgaaa
     3181 ggtgccatga ttgcgcgaat tttctcctct ctgtacggag tttgcccgat gcacgccatc
     3241 tccttacatt ctctcgctta tcgccgtttc gcgcgaaacg tttccctttc tatgttactg
     3301 ctcatgcggt gaagttaacg cacgctcact gcaggacaac agtaaaatca gagcgtttct
     3361 gcttttactg atgtctggcg gtcggagctg gtgaccagtt tgacccatat ctcatggggc
     3421 agggttttcc accttgtccg gtattcttac ttccccgaaa cgggtttgcg cttatgaaat
     3481 caatgaatat tgccgccagt agtgaactgg tatcccgact ttcttctcat cgccgcgtgg
     3541 tggcgttggg agatactgat tttacggacg tcgcggcagt cgtcattacc gctgcggata
     3601 gtcgcagtgg cattcttgcg ttgcttaagc gcaccggttt tcatctaccg gtgtttttgt
     3661 attccgaaca tgctgttgaa ttacctgcgg gcgttacggc ggtaatcaac ggcaacgagc
     3721 agcagtggct ggagctggaa tccgcagcct gtcagtatga agagaatttg ctgccaccgt
     3781 tttatgacac gctgacgcag tacgttgaga tgggcaacag cacctttgct tgccctggac
     3841 atcaacatgg tgcgttcttt aaaaagcatc ctgccggacg ccatttttac gatttctttg
     3901 gtgagaacgt ctttcgcgcc gatatgtgta acgctgacgt aaaattgggc gatctgctta
     3961 ttcatgaagg atcggcgaaa gatgcgcaga aattcgcagc caaagtcttt catgccgata
     4021 aaacctattt tgtgctgaac ggcacatcgg cagcgaataa agtggtgacg aatgcgctgt
     4081 taacgcgtgg cgatctggtg ctcttcgacc gtaacaacca taagtcgaat catcacggcg
     4141 cgctgattca ggcggggacg acgccggtct atctggaagc ttcacgtaac ccgtttggtt
     4201 tcattggcgg tattgatgcg cactgtttta atgaagagta tctgcgccag caaattcgcg
     4261 acgttgcgcc agaaaaagcc gacctgccgc gcccgtttcg cctgacgatt attcagctgg
     4321 gaacctatga cggcactgtc tataacgccc gtcaggtgat cgataccatt gggcatctgt
     4381 gtgattacat tctgtttgat tccgcgtggg tcggttacga acagtttatc tcgatgatgg
     4441 cggatagctc gccgctgctg ttagaactta acgaaaacga tccggggatt tttgtgaccc
     4501 agttggtgca caaacagcag gctggattct cacagacgtc gcagatccat aaaaaagata
     4561 accatatccg cggacaggcg cgtttttgcc cgcataagcg gttgaataac gcctttatgc
     4621 tccatgcttc taccagccct ttctatccgc tgtttgctgc actggatgtt aacgccaaaa
     4681 ttcatgaagg ggagagtggg cgtcggctgt gggctgagtg cgttgagttg ggtattgagt
     4741 cgcgcaaggc tattcttgcg cgctgtaagc tgttccgccc gtttatcccg cccgttgttg
     4801 atggcaaatt gtggcaggat tatccgacat cagtgttagc cagcgaccgc cgttttttca
     4861 gttttgagcc gggggcgaag tggcacggct ttgaaggata tgccgcggat cagtattttg
     4921 ttgatccgtg caagctgtta ctcactacac caggtatcaa tgccgaaacc ggcgaatata
     4981 gcgactttgg cgttccggcg acgattctgg cgcactatct gcgtgagaac ggcattgtgc
     5041 cggagaagtg cgatctcaac tccattctgt tcttattaac tccggcggaa agccacgaga
     5101 agctggcaca actggtggcg atgctggcgc aatttgaaca gcatattgag gatgactcgt
     5161 cgctggctga ggtgttgccg agcgtttata acaaatatcc ggtgcgctat cgcgactaca
     5221 ccctgcgcca gttgtgtcag gagatgcacg atctgtatgt cagtttcgac gtcaaagacc
     5281 tacaaaaagc gatgttccgc cagcagagtt tcccgtcagt ggtgatgaac ccccaggatg
     5341 cgcatagcgc ttatattcgc ggtgaagtgg agttggtgcg gattcgtgat gccgaagggc
     5401 gaattgcggc agaaggggcg ttgccttatc cacctggcgt gctttgcgtg gtacccgggg
     5461 aagtctgggg tggggcggtt caacgttatt tccttgcgct ggaagaaggc gtgaatttgt
     5521 tgccgggatt ttcgccggag ttgcaaggtg tctatagcga aaccgatgcg gatggcatga
     5581 aacgattgta cggttatgtg ttgaagtaag aataaaaaaa cgggtcacct tctggcgacc
     5641 cgtttttctt tgcgtaatta gtggctaacc gtctgtgtgc ctgtcgggac acgaacgtgt
     5701 ttatatttga acatcgccat gaacgcgaag gccagaacca cggaataacc agcgaaaatc
     5761 aaccatacgg tctgccagtc ggtaatgccg ttttgggtgt acatctcaac aactttaccg
     5821 ctcacgatgc cgccgaggat acagccgaag ccgttagtca tcatcaggaa catcccttgt
     5881 gcactggcgc gaattgccgg gctaacttct ttttcgacaa acaccgaacc agagatgttg
     5941 aagaagtcga atgcgcaacc gtaaacgatc atcgacagta ccagcagtac agtaccgaac
     6001 ggagtcgggt cgccgtaagc aaacagcgca aaacgcagga tccacgccac aatactgatc
     6061 atcattacgt tcttaatacc gtagcggctt aagaagaacg ggatggtcag aatgaacagg
     6121 gtttcagaga tctgcgaaat cgacatgatg attgacgcat gctgcacgat gaagctgctg
     6181 gcaaacatcg gatctttgtc gaagctgtgc aggaaggtat taccgaacat gttggtaatc
     6241 tgcagttccg cgcccagcag cattgagaag atgaagaaga ttgccatacg cttgttttta
     6301 aacagcgcga atgcatcgag gcccagcagg gttgtccagc tctgattcgc ttgctgttta
     6361 gcaaccggaa tatgcggcag agtcagggta aacagaacca gaatggcgga aagtgctgcg
     6421 ccaatataca gctgcatgtg gcttaattcg aagccagaca ggctcaccac ccacattgcc
     6481 atgataaagc cgatggtgcc ccagatacgg attggcggga agtcagtaac gatatccatc
     6541 ccggcatttt gcaggcgata gtaagagatg gtgttgatta acccaagcgt tggcatataa
     6601 gcaaacgagt taatcaatat cacaaggaac atcgcttccg gcgtcgtgac ctgtgccgcc
     6661 atgaacagcg tgatagcgcc aatggtgtgg caaatggcat atacccattt cgcacttaac
     6721 catttgtcgg ccacaatccc cagcagcgca ggcataaata ccgcggcgat acccagtgag
     6781 ctataaactg cgccaataga agcaccgtca aacttcaggg taacaaacat ataggagccg
     6841 agggtcgtca gccaacttcc ccacagacag aactgcagaa aagagaggat tttcagctgc
     6901 agcttaagat tcatgttaat ttcctcacat cgtgatgcgg atgagtgttt gaaaaagcac
     6961 atttgaccgt gcgagatgat gggattctat caacttcgcg gactggtttt ttgttacctg
     7021 tggcaaataa ttgcaaacat tcccaatttg caaaacgaaa ttgtgatgtg gataacattt
     7081 ttgcccctga gcatcgtcag gggcggttaa tggaattatc ggcggcggta ggatttttgc
     7141 gcggtattca ctttataaag ataacggcga gattccgcag agggatggcg ggtcgtcagc
     7201 gtctgataaa catcgcccgg cgtcatggtg ttaataatat tggcagcctg aatcttatca
     7261 ttcgaaaaga ctcgcagcac gctgcctgcg ccgccgttat aggcggtgat gacggcataa
     7321 cgccgcgatg ttgggttatc aattccgccg agataaacat tgttcagcat cgccagatac
     7381 gcggtgccgg tatcaatatt gctggcagga tcaaacaaga aactgcggct cggcgtgccg
     7441 gatttcccct gcgagcggaa cacatctttc ccggcagtat tttgtaccac ctgcattaat
     7501 cccagcgcat cggaacggct gaccgcatac gggttaaagg aagattcagt ctgcataatt
     7561 gccagaatca gcgactcatc aacgccatat ttccgtgacg cctggcggac catgccgaga
     7621 tatttgtgcg cacgtttatc aaggtggttc ggcaccatgt taatggtgac gctgtagatg
     7681 atacgtagcc cgttgctgcg gcttttcaga cggtttttca gcagataatc cgcgaagttg
     7741 cttgcgcgac cttcccagcg aatcggctgc ccggtgttgt ccaccacctg accgtaaagg
     7801 aaaggttctt tcgaaattgt aatatcatca acgtcggaat agagatcgac cgaactcgga
     7861 tcgtcaccca tcagcaacgt tttgataatt gcccggcgca aatgcgcggc aggttctgtc
     7921 ccggcgatgg tttcgatagt aatcgtaccg tcatcgaagt tgatgtggct gcgggtctga
     7981 tattgatcgg tgtatttcac gtagtcctta ggaccggcga tcaccacctc tttgaagccc
     8041 cagatgttct caatattgtg ggcaaattgc cccatcagaa tatcaaaacc gttggtatct
     8101 ttgacccagg cttcgttata ggtatcgcct tttttggtcg tcgaacagga gatgagcaac
     8161 ggcgcaatca aagccagcgc gagatatttt ttcatcattc cgggtgcgtg ttgtgtttgt
     8221 ttttatgcaa gctgccggag gcgtgctccg gcactgtttt tattttttat cttccggcgt
     8281 atagccctcg atatgcacct ctttaccctc gaacaggaag ttgaccatct cctgctccag
     8341 cagcttgcgg tgctcggcat tcatcatgtt gagtttcttt tcattaatca gcatggtttg
     8401 tttgtgctgc cactgcgccc aggcttcttt ggagatctcg ttatagatgc gttttcccag
     8461 ctcgccgggg tacagctgaa aatcctgacc ttctgcttca cgttgcagga aagtacaaaa
     8521 aatcgttctg ctcataaatc atcctcttta tcgactcacg cgctaaaccg gcgcgccagt
     8581 gcgtaactgc tgtaacaaac gctccacggg agccgccagg ccaactgacg gcggttgcgc
     8641 taagttatac cagagcgcat tgccttcatc catgcagccg gtgaatgacg acacgggaag
     8701 ccacataggc acaatatcta agtggaaatg gctgaaggta tggcgaaacg cggtcagttg
     8761 cgtcaggtta tcggcagcaa tctgccgttg tgccagccaa tgccgcaaac tttcttcgtc
     8821 ggcaaactgc gggaaacagt ataaaccgcc ccacaatccg ctcggcggac gctgcgccag
     8881 caatacttca tcttcgtgct gtaacaggag aaagtagccg gtgcgctccg gcagcgtctg
     8941 tttcggtttt ttgcccggat aaagcgacca gctattgttg gtggcggcaa tacatccgtt
     9001 ttgtagcgga cagagcgaac atttcggctt cgaacgcgtg caaatcatcg cgcccaaatc
     9061 catcatcgcc tgattaaacc gttccacgcc gaccgcgggc gtcacctgct cgctcaaact
     9121 ccatagttta ttctcgacct cttttttccc aggccagccg cttacagcat agcagcgcgc
     9181 cagcacgcgt ttgacgttac cgtcgagaat cggaaagtgc ttacccagag aaagcgagag
     9241 aatcgcgcct gcggtggaac gcccgacgcc cggtaacgcc gcgacttctt caaaggtttc
     9301 cgggaattta ccaccgtgta aggtcgccac ttgttgtgcc gctttatgca ggttgcgcgc
     9361 gcgggcgtaa tagccaagcc cggtccacaa gtggagaact tcgtcgagcg gcgcattggc
     9421 gagatcggtc accgtcggga agtgcgccat aaagcgttca aaatagggga taacggtcgc
     9481 aacctgagtt tgttgcaaca tcacttctga gagccatact ttgtagggcg tcttgtcaat
     9541 ttgccagggc agcgttttcc gcccgtattt atcgtaccag tccagaacct gggctgaaaa
     9601 ttgcgacgct tgcatggtca ccgacttcac tgttgttggg ggcaagattg cagcacagcg
     9661 acggcagggt gtaaaccgga actttccgca gccacggcct tatcatgctt gcatccggca
     9721 actaactttg gataatgccc gttttcagaa cactttcaca agcgactaaa tctttatgaa
     9781 aaacgacgtc atttcaccgg aatttgatga aaacggccgc ccactgcgcc gtatccgtag
     9841 ttttgtgcgc cgccaggggc gactgaccaa aggccaggaa catgcgctgg aaaactactg
     9901 gccggtgatg ggcgttgagt tcagtgaaga tatgctggat ttccccgcgc tttttggccg
     9961 tgaagcgccg gtgacgcttg agattggttt tggcatgggg gcgtcgctgg tggcaatggc
    10021 taaagatcgc cccgagcagg acttcctcgg cattgaagtg cattcaccgg gcgttggtgc
    10081 gtgcctggct tctgcgcatg aagaaggttt aagcaacctg cgcgtgatgt gtcacgatgc
    10141 ggttgaagtg ctgcataaaa tgattcctga caattcattg cgcatggtgc agctcttttt
    10201 ccctgacccg tggcacaaag cgcgccataa taaacgccgt atcgttcagg tgccgtttgc
    10261 cgaactggta aaaagcaaac tgcagctggg gggcgtattc catatggcga ccgactggga
    10321 accttatgcg gaacatatgc ttgaagtgat gtcttctatt gacggttata aaaacctgtc
    10381 agagagcaat gattacgtac cgcgtccggc atcacgtccg gtgacgaaat ttgaacaacg
    10441 tggtcatcgt cttggtcacg gagtatggga cttaatgttc gagagggtga aataatggca
    10501 aagaaccgta gccgtcgtct gcgtaaaaaa atgcacatcg acgaattcca ggaattagga
    10561 ttttcggtgg catggcgatt cccggaaggt acatcggaag aacagattga taaaatcgtt
    10621 gatgacttta ttaacgaggt tatcgaaccg aacaaactgg cctttgacgg cagcggttat
    10681 ctggcctggg aaggtctgat ctgcatgcag gaaatcggca aatgcaccga agaacatcag
    10741 gcgattgtgc gtaagtggct ggaagagtgc aaactggatg aggtacgcac cagcgaactt
    10801 ttcgacgttt ggtgggacta agaaagcata cgggcgatga caaatgcaaa actgcctgat
    10861 gcgctacgct tatcaggcct acagagatgc acgatcgagt aggcgggata aggcgtttac
    10921 gccgcatccg gcatggaaaa cgcgtacttt gttatcaatc tggggccagc atatgctggc
    10981 ctgatttgtt cttgagggaa gactatgatg cgcaaaatgc tgctggcggc agcactttca
    11041 gtgacggcaa tgaccgctca cgccgactac cagtgcagcg tcacgccgcg tgacgatgtg
    11101 attgtcagcc cgcaaaccgt gcaggtgaag ggcgaaaacg gcaatctggt gatcacgcca
    11161 gacggcaacg tgatgtataa cggtaagcaa tattccctga atgccgccca gcgcgagcag
    11221 gcgaaggatt atcaggctga actacgtagc acgctgccgt ggattgatga aggcgcgaaa
    11281 agccgcgtcg agaaagcccg tattgcgctg gataaaatta tcgttcagga gatgggcgac
    11341 agcagcaaaa tgcgcagccg tctgaccaaa cttgatgcgc agcttaaaga gcagatgaat
    11401 cgcattatcg aaacgcgcag cgatggcctg acgttccact ataaagccat tgatcaggtt
    11461 cgcgccgaag gccagcaact agtgaatcag gcaatgggcg gaattttaca ggacagcatt
    11521 aatgaaatgg gcgcgaaagc ggtgctgaaa agcggcggta acccattaca gaatgtgctg
    11581 ggaagcttgg gcggtctgca atcctcaatc caaaccgagt ggaaaaagca ggaaaaagat
    11641 ttccagcagt ttggcaaaga tgtttgtagc cgcgttgtga ctctggaaga tagccgcaaa
    11701 gccctggtcg ggaatttaaa ataatcctct attttaagac ggcataatac ttttttatgc
    11761 cgtttaattc ttcgttttgt tacctgcctc taactttgta gatctccaaa atatattcac
    11821 gttgtaaatt gtttaacgtc aaatttccca tgcaaagcta agggataatg cgtagcgtcc
    11881 acgtaactgg aggaatgaaa tggagttttt caaaaagacg gcacttgccg cactggttat
    11941 gggttttagt ggtgcagcat tggcattacc caatatcacc attttagcaa ccggcgggac
    12001 cattgccggt ggtggtgact ccgcaaccaa atctaactac acagcgggta aagttggcgt
    12061 agaaaatctg gttaatgcgg tgccgcaact aaaagacatt gcgaacgtta aaggcgagca
    12121 ggtagtgaat atcggctccc aggacatgaa cgataatgtc tggctgacac tggcgaaaaa
    12181 aattaacgcc gactgcgata aaaccgacgg cttcgtcatt acccacggta ccgacacgat
    12241 ggaagaaact gcttacttcc tcgacctgac ggtgaaatgc gacaaaccgg tggtgatggt
    12301 cggcgcaatg cgcccgtcca cgtccatgag cgcagacggt ccattcaacc tgtataacgc
    12361 ggtagtgacc gcagctgata aagcctccgc taatcgtggc gtgctggtgg tgatgaacga
    12421 caccgtactg gacggtcgcg atgtcaccaa aaccaacacc accgacgtag cgaccttcaa
    12481 gtctgttaac tacggtcctc tgggatacat tcacaacggt aagattgatt accaacgtac
    12541 cccggcacgt aagcacacca gcgatacgcc attcgatgtc tctaagctga atgagctgcc
    12601 gaaagtcggc atcgtttata actacgctaa cgcatccgat cttccggcta aagcactggt
    12661 agatgcgggc tatgatggca tcgttagcgc tggtgtgggt aatggtaacc tgtataaatc
    12721 cgtgttcgac accctggcaa ccgccgcgaa aaacggcact gcagtagtgc gttcttcccg
    12781 cgtaccgacg ggtgctacca ctcaggatgc tgaagtggat gatgcgaaat acggcttcgt
    12841 cgcctctggc acgctgaacc cgcaaaaagc gcgcgtcctg ctgcagctgg ctctgacgca
    12901 aaccaaagat ccgcagcaga tccagcagat tttcaatcag tactaatcac ctcgtcccgg
    12961 catcgtgccg gggctttttc acttcagact tacgtccatt gccaattttc attaccctaa
    13021 tgataatcac cggaataaat tattccgcgc gagggttttc gggtgaaaaa gcaatggatt
    13081 gttggcacgg cgctgcttat gttgatgact ggtaatgcct gggcagatgg cgaaccgcca
    13141 actgaaaata tcttaaaaga tcaattcaaa aagcagtatc acggcattct caagcttgat
    13201 gccatcagct taaaaaatct tgatgctaag ggtaatcagg ccacctggtc agcggaaggc
    13261 gatgtctctt ccagtgacga tctctatacc tgggtcggtc agttggcaga ttacgaactg
    13321 ctcgaacaga cctggacgaa agataaaccg gtaaaattct cggcgatgtt aaccagtaaa
    13381 ggaacgcccg cgtctggctg gtcggtgaac ttttactctt ttcaggcggc agccagcgat
    13441 cgtgggcggg tggtcgacga tatcaaaacg aataataaat atctgatcgt gaatagcgaa
    13501 gatttcaatt atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt
    13561 cctgcgctgg aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca
    13621 gcggatgcat attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt
    13681 aaaaaaattc accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg
    13741 gttaaatatg acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa
    13801 gagtgttatg aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga
    13861 cagacttttc tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc
    13921 gaaaaagggc aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg
    13981 attttgatga aaattgacga tatcaaccaa gctaatgaac gctagaaaaa agataccgaa
    14041 caactgcggc gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat
    14101 aaaaagttat ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg
    14161 atatttgcgg aatgtaagtg atcatcatca acgctactag caatgcagca taaaatagca
    14221 gcaatggttt gattaaatgt tgaatcttca cacccgagat agaacaacca acaaatagcg
    14281 cactacccac cggcggtgtt aacagcccaa tacaaaggtt ggccaccatc ataataccga
    14341 aatgtactgg gtccattccc agctcttgtg caatcggcaa gaagatcggt gtaaagatta
    14401 gtacagccgg tgtcatgtcc ataaagatgc cgacaattaa taagacgata ttaatcagca
    14461 gcaatatgat cagtgggtta tcagaaatcc ccatcaatgc gtcgctgatc atataaggaa
    14521 tatcagcatt agtcattgcc caggacatgc ccacggaaaa accgatcaac agtaatacaa
    14581 tcgaagtggt aacaaccgac tccagaatca gtttgggtaa atggcgccat tttacttccc
    14641 ggtaaatcag caccgagaga ataaatgtgt agacaacggc aattgctgac gcttcagttg
    14701 cggtaaaaat gccgcccaga atgccgccca tcacaatgaa caccagaagc aaacttggta
    14761 aggcatccag aaacgcttta caggcctgag caaaggttgc tctttctgaa agcgggtagc
    14821 cacggcgttt agcaataatg ccgcagacta tcattaccgc aagacccatt aagatccctg
    14881 gcaaatatcc cgccataaac agcgatgcta cagaaacgcc gccagctgtc agggaaaaca
    14941 caattaacac gtttgagggc ggtatcagta atccagtaat acaagatgag acgtttacag
    15001 cggtagaaaa tgccggatca taaccttctt tggtctgaat gggattcagt g
//
LOCUS       DAJTYM010000090        15000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTYM010000090 DAJTYM010000000
VERSION     DAJTYM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..565)
                     /locus_tag="OO575_003015"
     CDS             complement(<1..565)
                     /locus_tag="OO575_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6665895.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDT"
     gene            839..1306
                     /gene="slyB"
                     /locus_tag="OO575_003016"
     CDS             839..1306
                     /gene="slyB"
                     /locus_tag="OO575_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6665896.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(1353..1787)
                     /gene="slyA"
                     /locus_tag="OO575_003017"
     CDS             complement(1353..1787)
                     /gene="slyA"
                     /locus_tag="OO575_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6665897.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            1988..2224
                     /locus_tag="OO575_003018"
     CDS             1988..2224
                     /locus_tag="OO575_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6665898.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2227..3084
                     /locus_tag="OO575_003019"
     CDS             2227..3084
                     /locus_tag="OO575_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6665899.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3084..5096
                     /locus_tag="OO575_003020"
                     /pseudo
     CDS             3084..5096
                     /locus_tag="OO575_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5097..5618)
                     /gene="sodC"
                     /locus_tag="OO575_003021"
     CDS             complement(5097..5618)
                     /gene="sodC"
                     /locus_tag="OO575_003021"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6665900.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(5699..6595)
                     /locus_tag="OO575_003022"
     CDS             complement(5699..6595)
                     /locus_tag="OO575_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6665901.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(6644..6883)
                     /locus_tag="OO575_003023"
     CDS             complement(6644..6883)
                     /locus_tag="OO575_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6665902.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            6986..7585
                     /gene="nemR"
                     /locus_tag="OO575_003024"
     CDS             6986..7585
                     /gene="nemR"
                     /locus_tag="OO575_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6665903.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7622..8719
                     /gene="nemA"
                     /locus_tag="OO575_003025"
     CDS             7622..8719
                     /gene="nemA"
                     /locus_tag="OO575_003025"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6665904.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            8800..9207
                     /gene="gloA"
                     /locus_tag="OO575_003026"
     CDS             8800..9207
                     /gene="gloA"
                     /locus_tag="OO575_003026"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6665905.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            9310..9957
                     /gene="rnt"
                     /locus_tag="OO575_003027"
     CDS             9310..9957
                     /gene="rnt"
                     /locus_tag="OO575_003027"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6665906.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10050..12164
                     /locus_tag="OO575_003028"
     CDS             10050..12164
                     /locus_tag="OO575_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6665907.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12174..13466
                     /locus_tag="OO575_003029"
     CDS             12174..13466
                     /locus_tag="OO575_003029"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665908.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            14416..14508
                     /locus_tag="OO575_003030"
     CDS             14416..14508
                     /locus_tag="OO575_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR6665909.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(14552..>15000)
                     /locus_tag="OO575_003031"
     CDS             complement(14552..>15000)
                     /locus_tag="OO575_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6665910.1"
                     /translation="TTVSKNHVMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRS
                     ILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVC
                     GYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3409 a         3969 c         3947 g         3675 t
ORIGIN      
        1 cggtatcgta gccgccaacc agctgcccag gaatgagcag tgacagattg gcgatgccgc
       61 caatattgag caccattcgt cgctcggttg ggtgagccag caaggcatga tggaacgcag
      121 gtaccagcgg tgcgccttgt cctcccaagg caatatcgcg acggcgaaaa tcaccgacaa
      181 ccgtaattcc ggtgcgtgcc acaatttgat tgttatcgcc aatctgtaaa gtgtgtggtg
      241 ccacgcccgt cggttcatgc cagacggttt gaccgtgaca gccgatcgca actatatctc
      301 tcgcctgcag gttttgttcc ttaagcaagg cattgaccgc atcagcaaaa agtcgcccga
      361 gttgagtatc aagctgtcca aactgcgaaa gtgtaagctg ctggccctgg caaatatcca
      421 gtacagcctg tttcagagac accgggatcg gccagctcaa gcttgccagc tgtgcgaccc
      481 ggtgttcatc aattgtcgcc aacacaacat caacaccatc aaggctggtg cctgacataa
      541 cgccaataaa gcggcccgat ttcatagttc atcctttttc aatctgacgt ttgcgcacca
      601 ctcaaacata aacttttcgt gaataccatg cggaatgacc gatttttacc gttggtagta
      661 aaacattatc ttcaaatcaa taatcatcat gaatgttttg tttataattg gttgatccta
      721 ctttcattat gatttgctca tatttggtag aacatgtaac catggattca catatgccat
      781 atactttgac catgagggat gcttgcgtgg cgtttcatgg tgaacaggag atttttcaat
      841 gattaaacgc gtattggttg tttcaatggt aggtctgtct cttgtcggtt gtgttaataa
      901 cgacaccctg tcaggggatg tttataccgc ttctgaagcg aaacaagtac agaatgtcag
      961 ctatggcacc atcgttaacg tacgtccggt acagattcag ggcggtgatg attccaacgt
     1021 tatcggtgca attggcggtg ctgttcttgg tggtttcctg gggaatactg ttggtggcgg
     1081 aaccgggcgt tctctggcta ctgcagcagg cgctgttgca ggtggcgtag ctggtcaggg
     1141 cgtacagagt gcaatgaaca aaacgcaggg tgtcgagctg gaaattcgta aagacgatgg
     1201 taataccatc atggtggtac agaaacaagg caacactcgt ttctctccgg gccaacgtgt
     1261 cgtactggcc agcaatggca gtcaggtgac cgtttctccg cgctaaataa gtttgcgtgt
     1321 ggtcaggtta ctgaccacac gcccccttca tttcaccctt tggcctgtaa ctcaatgata
     1381 ttatgctcaa gttttgcgat gagcgtaatc agttgctcca gttcctctgc ggagatgcca
     1441 tgtaatattt ccgcgcgggt tttgttaata acagcttcca tttcgctgat cagtggctct
     1501 gccttttccg tcagtttaat acgtttagcc cgacgatcgc tggcacaagt ttgacgcgaa
     1561 attaaccctt tttcttccag ttggtccaga gtacggacca gtgatggctg ctcgatgcca
     1621 atcgcttttg ccagttgaat ttgcgactgg tctggaggta actgatggat attgtgtaac
     1681 gtaacccaat gggtttgtgt taactccagc ggtttcaggc gatggtctat cagagcacgc
     1741 catatgcgca ccaaccgtgc cagatcagaa cctagtggcg attccaattt catctcctta
     1801 taattagctt gctaagatat tatgcggctt ttagaatagt gtgcagcaat tgtattgcta
     1861 aaacaaatgt attgctgcat ttggttaccg tcagacatat ttttcagaaa ttgcgcgtaa
     1921 atttttcgca cttaaagaat atttattaat ctaacgcaat atattcggtc gtaaaggaat
     1981 ctactttgtg aagtttatgc tcaatgcaac aggattgccc ttacaagatc tggtgttcgg
     2041 tgcgtccgtc tattttcctc cgtttttcaa agcattcgcg tttggattcg tcatctggct
     2101 tgtcgtacac cgcctgcttc gtggctggat ctacgccggc gatatctggc atcccttgtt
     2161 aatggattta tcgctgtttg cgatttgcgt ttgccttgct ctggcaatac tgattgcgtg
     2221 gtaactatgt caattaaaac aattaagtat ttctcaacaa tcattgtagc gatagttgcg
     2281 gttcttgccg gatggtggct gtggaattat tacatgcaat ctccgtggac acgcgacggg
     2341 aaaatacgcg cagaacaggt ttctatcacc ccacaggtgt cgggacgtat tgttgagctg
     2401 aatataaaag acaaccaact ggttaacgcg ggggaacttc tgctcacgat cgataaaacg
     2461 ccctttcaga tcgccgagct gaacgcacag gctcagctgg cgaaagcgca atctgacctc
     2521 gcaaaagcca ataacgaagc gaatcgtcgt cgtcatctct cacaaaattt tatttttgcc
     2581 gaagaactcg ataccgctaa cctcaatgtt aaagcgatgc aggccagtgt taatgccgca
     2641 caggcgacgc tgaaacagac acaatggcaa ctggcgcaaa cggaaattcg cgccccggtg
     2701 agtggatggg tgactaacct cactacccgc atcggcgatt acgctgacac cgggaaaccg
     2761 ctgtttgccc ttgtcgacag ccactcgttt tacgtcattg gttattttga agaaaccaag
     2821 ttgcgccata tccgcgaagg tgcaccagca caaattaccc tctatagtga taacaaaacg
     2881 ttacagggtc acgtttccag tatcggtcgg gcgatttatg atcagagcgt tgaaagtgac
     2941 tcaagcctta ttccggatgt gaaacctaac gtcccctggg ttcgtctcgc ccaactcgtt
     3001 cccgtccgct ttgcgctgga taaagttcca ggcgatgtca cgctggtgtc gggcaccacc
     3061 tgtagcattg ccgtaggtca ataatgaacg catcgtcatg gtccttgcgc aatttgccct
     3121 ggttcagggc cacgctggcg caatggcgtt atgcgttacg caataccatt gccatgtgtc
     3181 tggcgctgac ggttgcctat tatttgaatc tggatgaacc ctattgggcg atgacctcgg
     3241 ctgcagtggt tagctttccc accgttggcg gtgtcatcag caaaagcctc ggacgcatgg
     3301 ctggcagttt actgggagcc attgcggcac tgcttcttgc cggacatacg ctcaatgagc
     3361 cgtggttttt tctattgagc atgtcggcgt ggcttggctt ttgtacctag gcctgtgcgc
     3421 acttcacgaa taacgtcgcg tatgcatttc aactggcggg ctacacggct gccatcatcg
     3481 cctttccgat ggttaatatt actgaggcca gccagctgtg ggatatcgct caggcgcgcg
     3541 tttgcgaggt gatagtcggt attttgtgcg gcggcatgat gatgatgatc ctgcctagca
     3601 gttccgatgc tactgccctt ttaactgcat tgaaaaacat gcacgcccga ttactggaac
     3661 atgccagttt actctggcag cctgaaacaa acgatgccat tcgtgcagca catgaggggg
     3721 tgattgggca gatactgacc atgaatttgc tgcgtatcca ggctttctgg agccactatc
     3781 gttttcgcca gcaaaacgcg cgccttaatg cgctactcca ccagcaatta cgtatgacca
     3841 gtgtcatctc cagcctgcga cgtatgttgc tcaactggcc ctcaccgcca ggtgccacgc
     3901 gagaaattct cgaacaattg ctgacggcgc tcgccagttc gcaaacagat gcttacaccg
     3961 tcgcacgtat tatcgccccg ctacgcccga ccaacgtcgc cgactaccgg cacgtcgcct
     4021 tctggcagcg actacgttat ttttgccgcc tttatctgca aagtagtcag gaattacatc
     4081 gtctgcaaag cggtgtagat gatcatgcca gactcgcacg gacatccgct ctggctcgtc
     4141 ataccgacaa cgccgaagct atgtggagcg ggctgcgtac attttgtacg ttgatgatga
     4201 ttggcgcatg gagtattgca tcgcaatggg atgccggtgc caatgcatta acgctggcag
     4261 caattagctg cgtactctac tccgccgtcg cagcaccgtt taagtcgttg tcactgctga
     4321 tgcgcacgct ggtgttactt tcgctattca gctttgtggt caaatttggc ctgatggtcc
     4381 agattagcga tctgtggcaa tttttactgt ttctctttcc actgctggcg acaatgcagc
     4441 ttcttaaatt gcagatgccc aaatttgccg cactgtgggg gcaactgatt gtttttatgg
     4501 gttcgtttat cgctgtcact aatcccccgg tgtatgattt tgctgatttt cttaactata
     4561 atctggcaaa aatcgttggc gttgcgctgg cttggctagc gttcgccatt ctgcgtccag
     4621 gatcggatgc tcgtaaaagc cgccgccata ttcgcgcgct gcgccgggat tttgtcgatc
     4681 agctaagccg ccatccaaca ctgagtgaaa gcgaatttga atcgctcact tatcatcacg
     4741 tcagtcagtt gagtaacagc caggatgcgc tggctcgccg ttggttatta cgctggggtg
     4801 tagtgctgct gaactgttct catgttgtct ggcaattgcg cgactgggaa tcgcgttccg
     4861 atccgttatc gcgagtacgg gataactgta tttcactgtt gcggggagtg atgagtgagc
     4921 gtggcgttca gcaaaaatca ctggcggcca cacttgaaga attacagcgg atttgcgaca
     4981 gccttgcccg tcatcatcaa cctgccgccc gtgagctggc ggcaattgtc tggcggctgt
     5041 actgctcgct ttcgcaactt gagcaagcac caccgcaagg tacgctggcc tcgtaattac
     5101 ttaattacac cacaggcata gcgttcaccg ccaccgccca gcggtttagg ttgatcggac
     5161 atattatcgc cgccaacgtg gaccatcagc gccttgtctt tgatttcatc cagtgatttc
     5221 agacgaggcg cgatgacggc atcggtagct ttgccgtcat tattgacgac cagtgcaggc
     5281 agatcgccta aatgcccggt accttctggc ccttcatgtt taccggtatt ttgtggatca
     5341 agatgcccgc ctgcggattc cgcggcgctg gctttgccat ctttggtggc tggctggcag
     5401 cttcctttgg catgaatatg gaagccatgt tcaccggggg gtaatgcttt cagatcgggt
     5461 gaaaactcca gacctttatc ggtttcagta atggtgacgc taccaattga ctgcccaacc
     5521 ccttgcgacg tgacgaggtt catctcgact ttttcactgg cagcttgtgc gccggtcgca
     5581 acaaccagcg ccagaatggc cagactaaaa cgtttcatag gacctccgtt cattgtgcat
     5641 tcctgaataa gtgtaaacca gtgacacatt tttgaacggc ggctattcct aaaagtgctt
     5701 acggtacgtc gtaccccagt gcagctttac ggatacgaaa ccattgttga cgggtcattt
     5761 tcagtgtttc tgcttcgaca gctgcccgta cgcgctcaat tttacctgag ccgataattg
     5821 gcagcggctg cgacggtaaa cgtaataccc aggcgtaaac cacctgttca atcgagcccg
     5881 cgtttaactc ctccgccacc acagccagtt catcacgcag cggctggaaa taatcatcat
     5941 taaacagacg accaccacca aggcaggacc acgccatcgg acgaacacgc agttgttgta
     6001 gttggtcgag cgtgccatcc agcagtaacg gctgatgcac cggggatatt tccacctgat
     6061 tagtggcaag ggtaaacggc agacgtgatt gcaacagggc aaattgcgca ggcgtaaagt
     6121 tcgatacgcc aaaatgacgc actttgccgc tctgatgcag atgtttgaac gcgtccgcca
     6181 cttcatcggc atccattaac gggtctggtc ggtggattaa cagcagatcc agatgatcgg
     6241 tcgcgagatt aattagcgac tgttcggcgc tcttaatgat gtgattgcgg tcagtgatgt
     6301 aatgaccaat gacgttttct tcacgcgcgg tcgtcgcgat accgcattta ctgacgattt
     6361 ccatccgttc acgcaggtga ggtgccagtt tcagtgcctc gccaaacgcc gcttcgcact
     6421 gatagccacc ataaatatca gcatggtcca cggtggtcac gccgagatcc agatgctctt
     6481 caataaaact gaccagctgg cgggcggaca tattccagtc catcaatcgc cagtagccca
     6541 tcacaaaacg ggaaaactcc gggccttccg gcgcaatagt aatacgctga accataatcg
     6601 cttcctctta tcagatataa gaggagtata cgcaagatta ggttcaaaag agtgatggtt
     6661 gctccggttc gtctgatgac gctggcttat ttgcgcgtaa tttgcgcatt aatcgctgcc
     6721 gacaaaggcg cagcacctct tgtttttcgc catcgctcat tttattccag ttaaaacgct
     6781 catcccgact acgaaaacag ccgcgacaaa acccgcgttc gtcagactgg caaattcccc
     6841 ggcacgggct ctggacggga aagaactcta attgctccgc cacctcgccc tcctcagata
     6901 agattattac cattattgaa gctgttaatg tccaaagtag caactttgct tgcactagac
     6961 caactggtct actacactcc aacgcatgaa caaacacacc gaacatgata ctcgcgaaca
     7021 tctcttggcg acgggcgagc aactttgcct gcaacgtgga ttcaccggga tggggctaag
     7081 cgaattacta aaaaccgctg aagtgccgaa agggtccttc tatcactact ttcgctctaa
     7141 agaagcgttt ggcgttgcca tgcttgagcg tcattacgcc gcatatcacc agcgactggc
     7201 tgagttgctg caatccggcg aaggtaacta ccgcgaccgc atactggctt attaccagca
     7261 aacactgaac cagttttgcc aacatggaac catcagtggt tgcctgacag taaaactctc
     7321 tgccgaagtg tgcgatctgt cagaagatat gcgcagcgcg atggataaag gtgctcgcgg
     7381 cgtgatcgcc ctgctctcgc aggctctgga aaatggccgt gagaaccatt gtttaacctt
     7441 ttgtggcgaa ccgctacaac aggcacaagt gctttacgca ctatggcttg gcgcgaatct
     7501 gcaggccaaa atttcgcgca gtttcgagcc actggaaaac gcgctggccc atgtaaaaaa
     7561 cattattgcg acgcctgccg tttagcaggc attttttatc accagacgac cgggagcctt
     7621 tatgtcatct gaaaaactgt attccccact gaaagtgggc gcgatcacgg cggcaaaccg
     7681 tatttttatg gcaccgctga cgcgtctgcg cagtattgaa ccgggtgaca ttcctacccc
     7741 gttgatggcg gaatactatc gccaacgtgc cagtgccggt ttgattatta gtgaagccac
     7801 gcaaatttct gcccaggcaa aaggatatgc aggtgcgcct ggcatccata gtccggagca
     7861 aattgccgca tggaaaaaaa tcaccgctgg cgttcatgct gaaaatggtc atatggcctt
     7921 gcagctgtgg cacaccggac gcatttctca cgccagcctg caacctggcg gtcaggcacc
     7981 ggtagcgcct tcagcactta gcgcgggaac acgtacttct ctgcgcgatg aaaatggtca
     8041 ggcgatccgc gttgaaacat ccatgccgcg tgcgcttgaa ctggaagaga ttccgggtat
     8101 cgtcaatgat ttccgtcagg ccattgctaa cgcgcgtgaa gccgcttttg atctggtaga
     8161 gctccactct gctcacggtt atttgctgca tcagttcctt tccccttctt caaaccatcg
     8221 taccgatcag tacggcggca gcgtggaaaa tcgcgcacgt ttggtactgg aagtggtcga
     8281 tgccgggatt gaagaatggg gtgccgatcg cattggcatt cgcgtttcgc caatcggtac
     8341 tttccagaac acagataacg gcccgaatga agaagccgat gcactgtatc tgattgaaca
     8401 actgggtaaa cgcggcattg cttatctgca tatgtcagaa ccagattggg cggggggtga
     8461 accgtatact gatgcgttcc gcgaaaaagt acgcgcccgt ttccacggtc cgattatcgg
     8521 cgcaggtgca tacacagtag aaaaagctga aacgctgatc ggcaaagggt taattgatgc
     8581 ggtggcattt ggtcgtgact ggattgcgaa cccggatctg gtcgcccgct tgcagctcaa
     8641 agctgagctt aacccacagc gtgccgaaag tttctacggt ggcggcgcgg aaggctatac
     8701 cgattacccg acgttgtaat ccaacattgc gagcggcgta aagccgccgc tatactaaaa
     8761 caacattttg aatctgttag ccattttgag gataaaaaga tgcgtcttct tcataccatg
     8821 ctgcgcgttg gcgatttgca acgctccatc gatttttata ccaaagtgct gggcatgaaa
     8881 ctgctgcgta ccagcgaaaa cccggaatac aaatactcac tggcgtttgt tggctacggc
     8941 ccggaaaccg aagaagcggt gattgaactg acctacaact ggggcgtgga taaatacgaa
     9001 ctcggcactg cttatggtca catcgcgctt agcgtagata acgccgctga agcgtgcgaa
     9061 aaaatccgtc aaaacggggg taacgtgacc cgtgaagcgg gtccggtaaa aggcggtact
     9121 acggttatcg cgtttgtgga agatccggac ggttacaaaa ttgagttaat cgaagagaaa
     9181 gacgccggtc gcggtctagg caactaatct cctgccgggc gtgaactcat cgcgcccgca
     9241 tctttactgc atcgacaagt aatatttgtc ataatgcgcg ctgcaattta tccgtattaa
     9301 gagaatcaga tgtccgataa cgctcaactt accggtctgt gcgaccgttt tcgtggtttt
     9361 tatcctgttg tgatcgatgt tgaaacagcc ggatttaacg ccaaaaccga tgcgctgctt
     9421 gagattgccg ccatcaccct gaaaatggat gaacaaggct ggctgatgcc ggacaccaca
     9481 ttacatttcc acgtcgaacc atttgtcggc gcaaatttgc aaccagaagc cctcgccttc
     9541 aacggcattg acccgaacga tcccgatcgc ggcgcggtca gcgaatacga ggcgctgcac
     9601 gaaattttta aagttgtacg taaaggtatt aaagcgagcg gctgtaaccg cgccattatg
     9661 gtggcgcaca acgccaattt tgatcacagc tttatgatgg ccgccgcaga acgcgcctcg
     9721 ctgaaacgta acccgttcca ccctttcgcc acttttgaca ctgctgcact ggccgggctg
     9781 gcactcggac aaaccgtatt gtcaaaggct tgccagaccg ctggcatgga cttcgacagc
     9841 acccaggcgc actccgcgct gtacgacacc gaacgcactg ctgtgctgtt ttgtgaaatc
     9901 gtcaaccgct ggaaacgtct gggaggctgg ccgctacctg ccgccgaaga ggtgtaatcg
     9961 agtcgatgcc tgatgacatg caatgattca ggcatctata gtgaggctat tccacgcatc
    10021 aagcatgata ttcacgggga atagcgttaa tggcagataa tccagaccct tcatcgctcc
    10081 tgccggacgt gttttcaccg gcgacccgcg actggtttct tcgcgccttt aaacagccga
    10141 ccgctgtcca gtcgcaaacc tggcatgtgg cggcgcgagg cgaacatgcg ctggtgattg
    10201 caccgactgg ctccgggaaa acgctggcag catttctcta cgccctcgat cggctcttcc
    10261 gcgaaggcgg cgaagatacc cgcgaggcgc ataagcgtaa aacctcacgc atcctctata
    10321 tttcaccgat aaaagccctg ggcaccgacg ttcagcgcaa cttgcagatc ccgttgaagg
    10381 gtattgccga tgaacggcgg cggcgcggcg aaacggtaat tattgatgaa gtccacgcgg
    10441 tagcgggcag taaacgtggt gcgcatctgg cgttaagtct ggagcggctc gatgcgctgc
    10501 tccacacctc agcacagcga attggccttt ctgccactgt gcgctcagcc agcgatgtgg
    10561 cagcatttct tggtggcgat cgcccggtta cggtagtcaa cccgcccaca atgcgccatc
    10621 cgcagatacg aattgtcgta ccggtcgcca atatggatga tgtctcatcg gtcgccagcg
    10681 gcaccggcga agacagccat gccggccggg aaggctccat ctggccatat attgaaacgg
    10741 gtatccttga tgaagtgttg cgccatcgct cgaccattgt ctttactaat tcacgcgggc
    10801 tggcggaaaa actgacggca cgattaaatg agctttacgc cgcacgctta cagcgttccc
    10861 cgtctatcgc cgttgatgcg gcccatttcg agtcgacctc cggcgcaacc tctaaccgtg
    10921 tacaaagtag cgacgttttt attgcccgct cacaccacgg ctccgtctct aaagaacaac
    10981 gagcaatcac cgaacaggcg ctgaaatcgg gtgaattacg ctgcgtggtc gcaacctcca
    11041 gtcttgaact ggggattgat atgggcgcgg tggatctggt gattcaggtg gcaacgccgc
    11101 tttctgttgc cagtgggtta caacgcattg gtcgcgccgg acatcaggtt ggcggtgtat
    11161 ctaaagggct gtttttcccc cgtacccggc gtgatttagt cgattccgca gtcattgtag
    11221 agtgtatgtt cgcaggcagg ctggaaaacc tgacaccacc gcataatcct ctcgacgtcc
    11281 ttgcgcagca aaccgttgcc gccgcggcga tggatgcatt acaggtagac gaatggtact
    11341 cccgcgtacg ccgtaccgca ccgtggaaag atctgccaag acgtgttttt gacgccacgc
    11401 tggatatgct ttccgggcgc tatccctctg gcgatttttc tgcttttcgc cccaaactgg
    11461 tctggaacag ggagaccggg atattgaccg cccgacctgg cgctcaattg ttggcggtta
    11521 ccagcggcgg caccattccg gatcgtggca tgtatagcgt gttattaccc gaaggtgaag
    11581 aaaaggccgg ttcgcggcgg gtgggtgaac tggatgagga gatggtatat gagtcgcggg
    11641 tgaacgacat tatcactctc ggcgctacct catggcggat ccagcaaatc acccgcgatc
    11701 aggtgattgt gactcctgct ccgggtcgtt ctgcccagct ccccttctgg cgtggtgaag
    11761 gtaacggacg tccggctgaa ttaggcgaga tgatcggcga ttttcttcat ttactggcgg
    11821 atggcgcgtt cttttccggg actattcccc cgtggctggc agaagaaaat acgaacgcca
    11881 atattcaggg gttgattgac gagcaacgca acgcgacggg catcgttccg gggagtcgcc
    11941 atctggtcct cgaacggtgc cgtgatgaaa ttggcgactg gcgtattatt ttgcactctc
    12001 cctatggaag acgggtgcat gaaccctggg cgctggcgat tgccgggcga atacatgccc
    12061 tatggggcgc tgacgcgtct gtggtcgcca gtgatgacag cattgttgcg cgcattcctg
    12121 acactgatgg taaattgccc gacgccgcga tttttttgtt ttaaccagaa aagttgctgc
    12181 aaattgtccg cgaggcggta ggcagctcgg cacttttcgc cgcccgtttt cgcgaatgcg
    12241 ccgcgcgggc attattaatg ccgggccgca cgccgggcca tcgcaccccg ctttggcaac
    12301 aacggctgcg cgccagtcag ttgctggaaa tcgctcaggg atatccggat tttccggtca
    12361 ttctcgaaac cctacgcgaa tgtctgcaag atgtttatga tcttcccgca ctggaacgtt
    12421 tgatgcgtcg cctgaacggt ggcgaaattc aaatatccga tgtaacgacc actacgccct
    12481 cgcctttcgc cacaagttta ttgttcggct atgtcgcgga atttatgtac cagagcgacg
    12541 ccccgctggc agagcgccgg gcatccgtac tgtcgctgga cagcgagtta ctgcgcaatc
    12601 tactcggaca ggtcgatccg ggggaattac tcgacccgca ggtcattcgc caggtggaag
    12661 aagagttgca acgactggct cctggtagaa gagcgaaagg tgaagaagga ttgttcgacc
    12721 tgctgcgcga actggggcca atgaccgttg aagacctggc gcaacggcat acaggcagca
    12781 gtgaagaggt tgcgtcgtat ctggaaaatc ttcttgcagt aaaacgaatc ttcccagcga
    12841 tgattagcgg acaggagcgt cttgcctgta tggatgatgc cgccaggctg cgtgatgccc
    12901 tcggcgtacg gctaccagag tcattgccag agatttattt acatagagtc agttacccgc
    12961 ttcgcgacct ctttctgcgc tatctccggg ctcatgctct ggtcacggct gaacaactgg
    13021 ctcatgagtt tagtctcggt attgccattg tcgaagagca gcttcagcaa ctgcgtgaac
    13081 agggtctggt gatgaatctg caacaagaca tctgggtgag cgatgaagta tttcgtcgtc
    13141 tgcgtttgcg ctcgctgcaa gccgccagag aagcgacgcg tcccgttgca gccacgacct
    13201 atgcgcgatt gctgctggaa cgtcagggcg tattacccgc caccgatggt agcccggcgc
    13261 tctttgcctc aacatctcca ggcgtttatg agggcgtaga tggagtgatg cgggtgatcg
    13321 aacagcttgc cggagtcggt ttacccgcct cactctggga aagccagatc ctgcctgccc
    13381 gcgtacgcga ctattcgcca gaaatgctcg atgaattact ggcaaccgga gcggttatct
    13441 ggtcgggaca aaaaaaagct gggtgaagat gacggcctgg tggcactgca tctacagaaa
    13501 tatgccgcag aatcgttcac tcccgccgaa gcggatcagg cgaatcgttc ggcgctgcag
    13561 caagcgataa tcgctgttct ggctgacgga ggagcctggt ttgcacaaca aatcagccaa
    13621 cggatacgcg ataaaatcgt cgaatcggtt gattcctcgg cactgcaaga ggcgttatgg
    13681 gcgctggtct ggcaaggcgt cattaccagc gatatttggg caccgttacg cgccctcacc
    13741 cgcagcagtt ccaacgcacg cacctcaact cgccgcagtc accgggctcg tcgtgggcgt
    13801 cctgtctatg cacaacccgt ctcgccgcgg gtatcttaca acacaccaaa tctggctgga
    13861 cgctggtcgt tattgcaggt ggagccacta aacgataccg aaaggatgct ggcgctggcg
    13921 gaaaatatgc tcgaccgcta cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc
    13981 cctggcgggt ttccatcgat gcaaacactt tgtcgaagta tggaagactc cgggcgaatt
    14041 atgcgaggtc gttttgtaga aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt
    14101 gaccgattgc gcgatctggc gacacaagcc acgcaaacac gccactatac accagtggcg
    14161 ctctccgcca acgatccggc taatgtgtgg ggaaatcttc tgccctgggc tgcacatccg
    14221 gcaacgctgg taccaacgcg tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg
    14281 ttactctatc tggcgcaagg cggcaaaaaa atgctggtct ggcaggaaaa ggaggaatta
    14341 ctcgccccag agattttcca ggcgctgacc accgcactgc gtcgcgaacc aaggctgcgc
    14401 tttacgctaa cagaagtgaa tgatcttccg gtccggcaaa cgccgatgtt tacgctgctg
    14461 cgtgaggcgg gattttcaag ttcgccacaa gggctggatt ggggatagtt gacatcctcc
    14521 acgccctgaa agacgtggtt ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg
    14581 gttcctgctt caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc
    14641 ccgccgctaa aatgttacga gctccattta catcggcgtt cgctgtatat ccacatacct
    14701 ggcatctgaa tttactttgt gacaggcgat tttctttcgc tgtatgacca cagcacgcgc
    14761 aacgctggct tgtgtacgct ggcggaacag caagcacctg accgccacgc cagagctgct
    14821 tgtactcaag ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac
    14881 ctgattttgc ccggacattg cgccccggct ggctgaccgt acccgctgct gactttgaca
    14941 tgttgctgac cttcaaatcc tcaatgacta tcattacgtg gtttttgctg acggtcgttg
//
LOCUS       DAJTYM010000091        14979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTYM010000091 DAJTYM010000000
VERSION     DAJTYM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(162..1187)
                     /gene="tdh"
                     /locus_tag="OO575_003032"
     CDS             complement(162..1187)
                     /gene="tdh"
                     /locus_tag="OO575_003032"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6665911.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1197..2393)
                     /gene="kbl"
                     /locus_tag="OO575_003033"
     CDS             complement(1197..2393)
                     /gene="kbl"
                     /locus_tag="OO575_003033"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6665912.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2668..3525)
                     /gene="yibB"
                     /locus_tag="OO575_003034"
     CDS             complement(2668..3525)
                     /gene="yibB"
                     /locus_tag="OO575_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6665913.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3829..4761
                     /gene="rfaD"
                     /locus_tag="OO575_003035"
     CDS             3829..4761
                     /gene="rfaD"
                     /locus_tag="OO575_003035"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6665914.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4771..5817
                     /gene="rfaF"
                     /locus_tag="OO575_003036"
     CDS             4771..5817
                     /gene="rfaF"
                     /locus_tag="OO575_003036"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6665915.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5821..6813
                     /gene="rfaC"
                     /locus_tag="OO575_003037"
     CDS             5821..6813
                     /gene="rfaC"
                     /locus_tag="OO575_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6665916.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6810..8018
                     /gene="rfaL"
                     /locus_tag="OO575_003038"
     CDS             6810..8018
                     /gene="rfaL"
                     /locus_tag="OO575_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6665917.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8054..9196)
                     /locus_tag="OO575_003039"
     CDS             complement(8054..9196)
                     /locus_tag="OO575_003039"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6665918.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9205..10218)
                     /locus_tag="OO575_003040"
     CDS             complement(9205..10218)
                     /locus_tag="OO575_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6665919.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10243..10950)
                     /gene="rfaY"
                     /locus_tag="OO575_003041"
     CDS             complement(10243..10950)
                     /gene="rfaY"
                     /locus_tag="OO575_003041"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6665920.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10976..11983)
                     /gene="waaO"
                     /locus_tag="OO575_003042"
                     /gene_synonym="rfaI"
     CDS             complement(10976..11983)
                     /gene="waaO"
                     /locus_tag="OO575_003042"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6665921.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12026..12823)
                     /gene="rfaP"
                     /locus_tag="OO575_003043"
     CDS             complement(12026..12823)
                     /gene="rfaP"
                     /locus_tag="OO575_003043"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6665922.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12816..13940)
                     /locus_tag="OO575_003044"
     CDS             complement(12816..13940)
                     /locus_tag="OO575_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6665923.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13937..>14979)
                     /gene="rfaQ"
                     /locus_tag="OO575_003045"
     CDS             complement(13937..>14979)
                     /gene="rfaQ"
                     /locus_tag="OO575_003045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6665924.1"
                     /translation="RILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQDTIPIL
                     SENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAILVRLLN
                     ARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQTTMSYPP
                     ASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEVVLTSGP
                     DKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHIAAAVNT
                     PLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAADVIAAVD
                     KLLPSSTTGTSL"
BASE COUNT         4256 a         3369 c         3082 g         4272 t
ORIGIN      
        1 tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca gctatataaa
       61 agatcagcgc aattacgaac tctttattac actgacagga ttttacataa ttaacagatt
      121 atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca gctcagaata
      181 actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc atcgatagag
      241 aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc cgccatcttg
      301 taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc tttaaagatc
      361 actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc aatacgaccg
      421 ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga catttccaga
      481 ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag gttttctttc
      541 gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg gtattcgtta
      601 acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc agccataata
      661 cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa cgacagcgcg
      721 gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga gatattgtcg
      781 gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca gcccggacgg
      841 ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg acagttgcga
      901 cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt gaagcctttc
      961 acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac catcggcacc
     1021 gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt cccgcagatg
     1081 gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac agggacgtcg
     1141 gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc acatcctcag
     1201 gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg cgtaatttgc
     1261 tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt cggaacgacc
     1321 ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc aaatttctgc
     1381 gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc cgccagggta
     1441 aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag acggtcacgc
     1501 aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac aattgctggt
     1561 gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc aaccacttct
     1621 ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag cgtgccggtg
     1681 atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc attttcaccg
     1741 acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt atctgccaga
     1801 tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc atcggtggca
     1861 atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc aagctcctgc
     1921 atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc atcaataata
     1981 gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag cgtttcaaac
     2041 aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat ccccaggaat
     2101 gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat aaaacgcacc
     2161 gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat cagatccgga
     2221 tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct tccatcagcc
     2281 acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa caacccttcc
     2341 gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg cattgcgatt
     2401 ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg acgagatgat
     2461 gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc tctcaacacc
     2521 tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg attttactgc
     2581 attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca gttggcatca
     2641 aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc tcagcgcctg
     2701 catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc attttcctct
     2761 ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga caaaaatgcc
     2821 ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca gaactaattt
     2881 atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa tatatacatg
     2941 attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc ctttttttat
     3001 agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca atcctcgagt
     3061 tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg ttttcactaa
     3121 acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca aaacatactc
     3181 tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg taaatgactc
     3241 atctttttga attttttcga tacgacttct gatatatcta aatttctttt ttatatcgat
     3301 aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt tcaaatcggg
     3361 agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt aactaaaata
     3421 aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc aatcccctct
     3481 accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta ataagaccat
     3541 taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta tattatccat
     3601 tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac tattcacatg
     3661 caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt tgcaattagc
     3721 atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat gattacagac
     3781 attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat gatcatcgtt
     3841 accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga taaaggcatc
     3901 accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa cctggtggat
     3961 ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat ggctggcgaa
     4021 gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac caccgagtgg
     4081 gatggcaagt atatgatgga taacaactat caatactcca aagagctgct gcactactgt
     4141 ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg cggacgcacc
     4201 tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg ttactcaaaa
     4261 ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca gattgttggc
     4321 ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat ggcgagcgtc
     4381 gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt cgaaggtagc
     4441 gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa cctgtggttc
     4501 ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga atccttccag
     4561 gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata cattccgttc
     4621 ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac aaatctgcgc
     4681 gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga atacatggcc
     4741 tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg cccgtcttgg
     4801 gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg ctatccccag
     4861 gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg gatgccggaa
     4921 gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg cgaacgccgc
     4981 aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt accaaactcc
     5041 ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg ctggcgcggc
     5101 gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc ctggccgcta
     5161 atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac cgcacaagat
     5221 ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa atcatatacc
     5281 tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc gggtgcggag
     5341 tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa gcagctgatt
     5401 gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc gggcaatgag
     5461 attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc gggggaaaca
     5521 cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac taacgattct
     5581 ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta tggtccaagt
     5641 agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct gattaccggc
     5701 tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt gatcgacatt
     5761 actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga agcctgacgg
     5821 atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac gttgcccgca
     5881 ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt ggaagaaggg
     5941 ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt ggcaatacgt
     6001 cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc gtttcgtgaa
     6061 gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt aaaaagcgcg
     6121 gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca aaccgctcgc
     6181 gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca gcagcacgcc
     6241 gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc gcaaacccag
     6301 ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc tggcgaatat
     6361 gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga acactggcga
     6421 gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg gggcgcgccg
     6481 catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga agtattgccg
     6541 aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt agtgtcggtg
     6601 gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac ggtttatgga
     6661 ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg tcgttctaca
     6721 agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa tcttgaaata
     6781 ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc tctctcgaga
     6841 aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt accacctatt
     6901 ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca ataacgacaa
     6961 tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca tttctttttg
     7021 gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc cctgatgcag
     7081 gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta tgcacaatag
     7141 caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc gttttcttct
     7201 catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat tataaggact
     7261 atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct gactcgatgg
     7321 tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa taccgcattt
     7381 cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta tccagagggg
     7441 cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa caatggaagt
     7501 tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc acacataagg
     7561 agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat cgctatgcaa
     7621 atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt ggttatggtt
     7681 acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat ccagaatgga
     7741 cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt ggcactggtt
     7801 tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag tgtataaaaa
     7861 atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc ttactatctt
     7921 ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat ttattaggcg
     7981 tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag gctgcataat
     8041 gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt tgagttacgc
     8101 catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact gctgttaatt
     8161 caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc ataggttctt
     8221 ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca cgcgtactca
     8281 ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat ggttcctgga
     8341 actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc ttttcaggcg
     8401 gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc ctgacattgc
     8461 gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc acaactaatt
     8521 ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc aatatgcctt
     8581 tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag atattaagtt
     8641 cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt ccatttggta
     8701 caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag tataaactag
     8761 ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta tgcatatgta
     8821 taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc atgctattat
     8881 gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc aatattctct
     8941 gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta tatatgcgac
     9001 tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt gagtatcctg
     9061 gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc tgatacatcc
     9121 acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta accgtaaata
     9181 ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa ttgctgtaaa
     9241 aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt tgtgctcatg
     9301 ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat aaatattacg
     9361 aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta taccagtata
     9421 atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat ttttttcttc
     9481 aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt tagcatgatg
     9541 tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta taattgactc
     9601 atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt cccgtaaatt
     9661 aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac acaaacgctc
     9721 agcgctttta ctttgcatag catctacatc aattaccact gccccatatt catctttaaa
     9781 ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat caagatataa
     9841 taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat aaatagatac
     9901 tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat gatataattt
     9961 aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact ctttgtctaa
    10021 gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa gaattgaagc
    10081 aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga cattaaacgt
    10141 ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt taaattgagt
    10201 aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc tcttcaatcc
    10261 tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata taagatatgt
    10321 atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat caatgcgatc
    10381 ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc ttaaaccttt
    10441 ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat gctgatgcaa
    10501 ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca aatactcatt
    10561 taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga aatttaatgt
    10621 tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac cacgtacccg
    10681 atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt ttaagaaacg
    10741 ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac gagcagtgtc
    10801 aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt taaccctgca
    10861 agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta tgaaaacttt
    10921 caacccattt atcgttttat tatatatcat aatgaattat tttaacctta aatctttaga
    10981 agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa tgcttttgtt
    11041 taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta agtaaatctt
    11101 ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc ggataatcag
    11161 cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg tcatcattga
    11221 ctggattatc aactttgtct tttaattcat agttaatact atatcgggta ttatatttct
    11281 ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga tcaaggtggg
    11341 tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac gaaatgttat
    11401 ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat ccagaagcta
    11461 actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct ctttcagcta
    11521 caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta atactaccct
    11581 tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag aaataatctg
    11641 caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt aatgacttca
    11701 gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt gctaaatcag
    11761 aaaaatataa tttgtcttta tcactgatat aatctgtgaa aatatgaaat tcacaagaga
    11821 tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa agaaaatttc
    11881 tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga taatgaaaat
    11941 tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt ctgtatccaa
    12001 aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt tccctgattt
    12061 ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga atgtctttaa
    12121 gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc aggccaatat
    12181 tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct ggaacgcgcg
    12241 tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct tccttaccgg
    12301 aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta atgcccgcag
    12361 catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt acgcgaacat
    12421 ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt ggtgtcagat
    12481 cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg ccgaatgcca
    12541 ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc cattcgcggt
    12601 ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct ttcagggtcg
    12661 tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc agagtacggc
    12721 gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca aaaggatctt
    12781 tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc acccgttatg
    12841 atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata atgtcgcgca
    12901 ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa aatctcattc
    12961 aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc gacaatataa
    13021 tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat cgcttccagc
    13081 agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc cgccgccatt
    13141 aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac gccgcgtttt
    13201 tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag caatgtgttg
    13261 tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc acctttacgc
    13321 gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc attcttctta
    13381 cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg ataaatcccc
    13441 ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa atcggcgatt
    13501 tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt tgcccgctca
    13561 aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt ttcctgcgct
    13621 actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg cattttgttg
    13681 aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca ggcaaaatac
    13741 tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac ttcaaataca
    13801 tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc tcgggcggcg
    13861 actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa atatttatat
    13921 aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt aatttatcga
    13981 cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt cggtcacgct
    14041 gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg ttatttgacc
    14101 agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc gtattaactg
    14161 cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca tgatcgatta
    14221 acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt ggcgtctggc
    14281 atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg gacgtcagaa
    14341 cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg gcgttgtccc
    14401 agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt ccaacaccag
    14461 cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc atggttgtct
    14521 gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt tccaccacat
    14581 ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca gactgccgat
    14641 gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt atagcaacca
    14701 tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat accttgatga
    14761 gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt atgccgtaga
    14821 gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa agcagcacat
    14881 cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta gttaataaca
    14941 tatccccatg aaaacgcatc ttaatgagca aaattcttc
//
LOCUS       DAJTYM010000092        14926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTYM010000092 DAJTYM010000000
VERSION     DAJTYM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1176)
                     /locus_tag="OO575_003046"
     CDS             complement(<1..1176)
                     /locus_tag="OO575_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6665925.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1375..1459)
                     /locus_tag="OO575_003047"
     tRNA            complement(1375..1459)
                     /locus_tag="OO575_003047"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Leu,seq:caa)
     gene            1655..2674
                     /gene="ahr"
                     /locus_tag="OO575_003048"
     CDS             1655..2674
                     /gene="ahr"
                     /locus_tag="OO575_003048"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6665926.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2803..4305
                     /locus_tag="OO575_003049"
     CDS             2803..4305
                     /locus_tag="OO575_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6665927.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="OO575_003050"
     CDS             complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="OO575_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6665928.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="OO575_003051"
     CDS             complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="OO575_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6665929.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6873..8384
                     /gene="pepA"
                     /locus_tag="OO575_003052"
     CDS             6873..8384
                     /gene="pepA"
                     /locus_tag="OO575_003052"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6665930.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8518..8961
                     /gene="holC"
                     /locus_tag="OO575_003053"
     CDS             8518..8961
                     /gene="holC"
                     /locus_tag="OO575_003053"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6665931.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8961..11816
                     /gene="valS"
                     /locus_tag="OO575_003054"
     CDS             8961..11816
                     /gene="valS"
                     /locus_tag="OO575_003054"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6665932.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11870..13066)
                     /locus_tag="OO575_003055"
     CDS             complement(11870..13066)
                     /locus_tag="OO575_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6665933.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13259..13762
                     /locus_tag="OO575_003056"
     CDS             13259..13762
                     /locus_tag="OO575_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6665934.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13808..14224)
                     /gene="rraB"
                     /locus_tag="OO575_003057"
     CDS             complement(13808..14224)
                     /gene="rraB"
                     /locus_tag="OO575_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6665935.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14386..>14926
                     /locus_tag="OO575_003058"
     CDS             14386..>14926
                     /locus_tag="OO575_003058"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HCR6665936.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALT"
BASE COUNT         3841 a         3831 c         3832 g         3422 t
ORIGIN      
        1 catcaaccta cgctccccaa gatgttccgc cttatggatg taagctgctc gcacagagct
       61 acgctcctgg tgactcatct gccgctctac cgcatccctc gaccataatc ccgactcaat
      121 caacgaacta caagccatcg tcctgaagcc atgcccacaa acctcgacct tcgtgtcata
      181 ccccattacg cgcaaagcct tgttcaccgt attctcactc atcggcttac gtggatcgtg
      241 atcgcccaca aaaatcagct ctcgatttcc gctcatgctt ttgatctttt ccaaaatact
      301 tagggcctgc cgcgacaaag gtacaaggtg tggtgtccgc atcttcgagc cacgctgaga
      361 atgcttgact ccttccagtt gctcacgctc acctgggatt gtccacatag ccgtttcaaa
      421 atctatttct gaccagcggg caaaacgcag ctcgctcgaa cggatgaaaa ccaataaagt
      481 gagttcaacc gccagtcgag ttaacggtct gccagaatag tgatctatgc gatgaagtaa
      541 ttcaggtata cggttcagtt caagagctgc acggtgctgt cttttcgccg tagcaaccgc
      601 acctgcaatc tcttgcgcgg ggttgtagtc gattaagccg ctctgaacgg caaagcgcat
      661 aatcgcggta gtacgctgct gtaaacgggc ggcaacttcg agccgcccgg atgactcgac
      721 tgctttaatg ggtacaagaa gatcccgcgt cttcagttcc gcaatgttcc gcttaccgat
      781 agcagtaaag agattatcct ccagactttt caaaacacga gcgctatgcg atgccgacca
      841 cttctgattg ctggcgtgcc agtctctggc gactacttca aacgttatcg cctcttgttc
      901 ctgttctacc ttaacagctt tcttgttttc actgggatcg acaccattag ctaaaagctt
      961 acgagcctca tcccggcgtg ccctggcatc cgccaacgac acttcggggt atttccccaa
     1021 tgccagcatc ttctctttgc cgccaaagcg ataacgtagc cgccagtatt tggagccgtt
     1081 agggtgaacc agcaaaacca tgccttcacc gtcggtcagc ttataggctt ttgcttcagg
     1141 cttagccgaa cgaaccttca catcactcag agccatgatg agtatccttt caagggttct
     1201 gtgtgggtac aaacattatc gaaccgggaa atacccgcag ttgtacccgc atcagtaagt
     1261 tgatgtagat tgaatcaggt tgacttaggt tgagtgaaaa agcgaggaaa gccttgcgga
     1321 tactggattt taggcacaaa aaaagacgtc cgttgacgtc tattgatgtt ccgatggtgc
     1381 cgaaggccgg actcgaaccg gcacgtattt ctacggttga ttttgaatca actgcgtcta
     1441 ccgatttcgc cacttcggca ctgaagaggt atgcggaaaa cgttgtggat tatacctgtc
     1501 gcacgccacc atgcaagcgc cagcttgcac ccacctcgct aagtgctgaa attttcagca
     1561 ttacactcag cgatatgtaa cctttgtcac actccaggca ccccgccctg ccatgctcta
     1621 cacttcccaa acaacaccag agaaggcccc aaaaatgtcg atgataaaaa gctatgccgc
     1681 aaaagaagcg ggcggcgaac tggaagttta tgagtacgat cccggtgagc tgaagccaca
     1741 agatgttgaa gtgcaggtgg attactgcgg gatttgtcat tccgatctgt cgatgatcga
     1801 taacgaatgg ggattttcac aatatccgct ggttgccggg catgaggtga ttgggcgcgt
     1861 agtggcactc gggagcgccg cgcaggataa aggtttgcag gtcggtcagc gtgtcgggat
     1921 tggctggacg gcgcgtagct gtggtcactg cgacgcctgt attagcggta atcagatcaa
     1981 ctgcgagcaa ggtgcggtgc cgacaattat gaatcgcggt ggctttgccg agaagttgcg
     2041 tgcggactgg caatgggtga ttccactgcc agaaaatatt gatatcgagt ccgccgggcc
     2101 gctgttgtgc ggcggtatca cggtctttaa accactgttg atgcaccata tcactgctac
     2161 cagccgcgtt ggggtaattg gtattggcgg gctggggcat atcgctataa aacttctgca
     2221 cgcaatggga tgcgaggtga cggcctttag ttctaatccg gcgaaagagc aggaagtact
     2281 ggcgatgggt gccgataaag tggtgaatag ccgcgatccg caggcactga aagccctgtc
     2341 ggggcagttt gatctcatta tcaatactgt gaacgtcagc ctcgactggc agccttattt
     2401 tgaggcgctg acctatggcg gtaatttcca tacggtcggt gcggttctca cgccgctgtc
     2461 tgttccggcc tttacgttaa ttgcgggcga ccgcagcatc tctggttctg ctaccggcac
     2521 gccttatgag ctgcgaaagc tgatgcgctt tgccgcccgc agcaaggttg cgccgacaac
     2581 cgaactgttc ccgatgtcga aaattaacga cgccattcag catgtacgcg atggtaaagc
     2641 gcgctaccga gtagtcctga aagccgactt ctgatccctt tgccgggtgc tgtcgcccgg
     2701 cacttaaatc tctttggaag gcgtctcgca atctgctgaa tttagcggtg aagagtgcac
     2761 agccgttgcc tatactccag gcaggaaact ggaggaaatc tcatgagcga acccctgtta
     2821 attgcccgca cgccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg
     2881 ctgattactg gcgcaacggg gacgggtaaa accgttacgc tgcaaaaact ggcagagtca
     2941 ttgtcggaaa tcggcgtgcc ggtgtttatg gctgatgtga aaggcgatct gaccggtatc
     3001 gcgcaggcag gaacggcgtc ggaaaaactg ctcacaaggc ttaaaaatat cggcgtcaat
     3061 gactggcaac cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaaaggccac
     3121 acggtgcggg cgacggtttc ggatctgggg ccgctgttgc tggcgcgact gttgaatctc
     3181 aacgatgtgc aatctggcgt gctgaatatt atcttccgca ttgcggacga tcagggattg
     3241 ttgctgctcg actttaaaga tttgcgggcg attacccagt acatcggcga taacgccaaa
     3301 tccttccaga atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcgga
     3361 ttactgtcgc tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc
     3421 aaagactgga tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag
     3481 aaactttatc agatgccgaa actgtacgcc gccagcctgc tgtggatgct ttcagagttg
     3541 tatgaacaat tgccggaagc gggcgatctg gagaaaccaa aactggtgtt tttcttcgac
     3601 gaggcacatc tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg
     3661 ataaggctta ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat
     3721 attccggata atgtgttcgg ccagctcggt aatcgtgttc agcacgcttt gcgggctttt
     3781 acgccaaaag atcagaaagc agtgaagaca gcggcgcaaa ccatgcgggc caatccggca
     3841 tttgataccg aaaaggcaat ccaggaactg gggaccggcg aggcgttaat ctcgtttctc
     3901 gatgcaaaag gaagtccttc tgtggtggaa cgggcgatgg tgatcgcacc ttgttcgcga
     3961 atggggccgg tgacggaaga tgagcgtaat ggcctgatta atcactcccc ggtgtatggc
     4021 aaatatgaag atgaggtgga ccgcgagtcc gcctatgaga tgctgcaaaa aggctttcag
     4081 gccagtaccg agcagcaaaa taatcccccc gtgaaaggga aagaggtggc ggtggatgac
     4141 ggtattcttg gtggattgaa ggatattttg tttggcacta ccggaccacg cggcgggaag
     4201 aaagatggtg tggtgcaaac aatggcgaaa agcgccgctc gccaggtgac gaatcagatt
     4261 gtacgcggga tgttggggag tttgctgggg gggagaaaaa gataagtcag acgtgatctg
     4321 tgcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcgaac tcgtatttga
     4381 ctttgtcaac aataataaag ggagctttcg ctcccttgtt agattacgat tttctcatta
     4441 acagccacag gctgattaag aagaagctgg cacttggcaa cagtgcgccg atgatcggcg
     4501 ggatgccgta aaccaacgtc agcgggccga agatctggtc cagtacgtag aagacaaaac
     4561 cgaagctgat acctgtgacc acacgcacgc ccatcggtac gctacgcagt gggccaaaga
     4621 taaacgacag cgccatcagc atcatcaccg ccacggatag cggctggaag attttgctcc
     4681 acatgttgag ctgataacgt ccggcatcct gaccgctcga cttcagatac ttcacgtagt
     4741 tgtgcaaacc actgatggag agtgcatccg ggtccagcgc caccacgccc agtttgtctg
     4801 gcgtgaggtc ggttttccag gtgccgctca ccgtctgcga accggtaatc tgtttcggat
     4861 tggtcagatc agattcatca acctgcgaca gacgccagac tttatgttcc gggtcaaact
     4921 tcgcggtagc ggcataacgt acggattgca gacgacgatt ctcgttaaag gcataaatac
     4981 tgatgccacc taacacctcg tcacctttaa cccgctcaat gtagacgaag ttgttgccat
     5041 ctttcgccca taaaccttgc tgggtagaga gcaacgagcc gccgtacatc gcctgcgcac
     5101 ggtagttacg cgccatctgt tcgccctgcg gcgcgaccca ttcgccaatc gccatcgtca
     5161 gcaagaccag tggaatggcg gttttcatca ccgacagcgc tacctgcata cgggtaaaac
     5221 cagaagcctg catcaccacc agttcgctgc gctgcgccag catcccaaga ccaagcaacg
     5281 ccccaagaag agccgccatc gggaagaaga tctgcacatc tttcggcacg ctcagcaagg
     5341 tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc agctgatcga
     5401 caaatttgat aatgcccgac agcgacacca gcatgaacag cgtcatcatg atggtggtga
     5461 aaatagtttt accgatatag cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt
     5521 acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt tgagaacaat
     5581 cgccaaagcc agataaatca ggttaacggt ccacatccac agtgtcgggt ccagcttacc
     5641 tttaccgccg ttcgatttca gggaggtctg gatcaggaag aaaagtagat acagcagcat
     5701 ggctggcagc atcgacagta cgcgtccctg acgcgggtta accacgctca acggtacaac
     5761 cataagtgcc atcataaaca cggtgaatac caacgtgata cgccagttca gttctgcgcg
     5821 agcacgatcg gtgtcagtgt tccacaatgt gcgcatgtcc atctggtcgg tatcgttcgg
     5881 gtcgagcgcc accgcctggt gaccaatgat cgcctgataa tcctggaaat ccgtaatgcg
     5941 gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc tggttgagag tgacgacctg
     6001 ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc
     6061 attacctttt ggtcgaattt gtgcgaggaa cacatctttg aaatcgctgc cgtcaacgct
     6121 ttcgatgaac agcaccgagc tgccattagt cgcttgctgg aattgccctt gcgccagcgc
     6181 cgccatgcca gggttcgctt tcgcttctgc taacacttca tcctgatgac gcgatgacca
     6241 cggtcccgcc cacatcacgt taaccgccgc gacgattgcc gtgaataccg caaggatcat
     6301 tgccgctttc accagaaccg ctttgctcaa gccgcaggca tgcattaccg taatttcact
     6361 ttcggtatac agtttgccca gcgtcatcag cagcccgagg aacaggctta atggcaggat
     6421 aagctgcgcc atttccggca cgcccaaccc gagaagggag agcaccagat tcgccggaat
     6481 atcgccgtca accgctgcgc cgaggatcct cactaacttt tgacagaaga agatcaaaag
     6541 caagatgaag agtatcgcca gctggctttt gagcgtctcc cgcaccagat atcttatgat
     6601 tatcacttta aatacgcccg taaaaactcg tcttttgcag gattttagct tgtttcatgg
     6661 cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac
     6721 ggattcacct ctcagatttt gttctgacgt gccaatgccg taataacgtt aagattaaca
     6781 cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg caattctatc tgtagccacc
     6841 gccgttgtct ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc
     6901 cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc
     6961 cgattgcaga acagctcgat aaaatcagcg atgggtacat cagcgccctg ctacgccggg
     7021 gcgaactgga aggaaaaccg gggcagacat tgttgctgca ccatgttccg aatgtacttt
     7081 ccgagcgaat tctccttatt ggttgcggca aagaacgtga gctggatgag cgtcagtaca
     7141 agcaggttat tcagaaaacc attaatacgc tgaatgatac tggctcgatg gaagcggtct
     7201 gctttctgac tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg
     7261 tcgagacagc aaaagagacg ctctacagtt tcgatcagtt gaaaacgaat aagagcgaac
     7321 cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg
     7381 gtgagcgcgc gatccagcac ggtcaggcga ttgccgccgg gataaaagca gcgaaagatc
     7441 tcggtaatat gccgccgaat atctgtaacg ccgcttatct cgcctctcag gcgcgccagc
     7501 tggctgacag ctacagcaag aatgtcatca cccgcgttat cggcgaacag cagatgaaag
     7561 agctgggaat gcactcttat ctggcggtcg gtcagggttc acaaaacgaa tcgctgatgt
     7621 cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta
     7681 aaggtttaac cttcgactcc ggcggtatct ccatcaagcc ttcagaaggc atggatgaga
     7741 tgaagtacga tatgtgcggc gcggcggcag tttacggcgt gatgcgtatg gtcgcggagc
     7801 tgcaactgcc gattaacgtt attggcgtgc tggcaggctg tgaaaacatg cctggcgggc
     7861 gtgcctatcg tccgggcgat gtgttaacca ccatgtccgg tcaaaccgtt gaagtgctga
     7921 acaccgatgc cgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt gaacgttttg
     7981 agccggaagc agtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc gcgctgggtc
     8041 atcatattac tggtctgatg acgaaccata atccgctggc ccatgaactg attgccgcgt
     8101 ctgaacaatc cggtgaccgc gcatggcgct taccgctggg tgacgagtat caggaacagc
     8161 tggagtccaa ttttgccgat atggcgaaca ttggcggtcg tcctggtggg gcgattaccg
     8221 caggttgctt cctgtcacgc tttacccgta agtataactg ggcgcacctg gatattgcag
     8281 gaaccgcctg gcgttctggt aaagcaaaag gcgcaaccgg tcgtccggta gcgttgctgg
     8341 cacagttcct gttaaaccgc gctgggttta acggcgaaga gtaagtttgc gttttaccct
     8401 caccctaacc ctctccctaa gggagaggga accgatcgag cgcaatagaa attcattgct
     8461 catctttcag ggtgaggaat gcagcaatat ttaaatccac cacaagaagc cccatatatg
     8521 aaaaacgcaa cgttctacct tctggacaat gacaccaccg tcgatggctt aagcgccgtt
     8581 gagcaactgg tgtgtgaaat tgccgcagaa cgttggcgca gcggtaagcg cgtactcatc
     8641 gcctgtgaag atgaaaagca ggcttaccgg ctggatgaag ccctgtgggc gcgtccggca
     8701 gaaagctttg ttccgcataa tttagcggga gaaggaccgc gcggcagtgc gccggtggag
     8761 atcgcctggc cgcaaaaacg cagcagcagc ccgcgcgata tattgattag tctgcgaaca
     8821 agctttgcag attttgccac cgctttcaca gaagtggtag acttcgttcc ttatgaagat
     8881 tctctgaaac aactggcgcg cgaacgctat aaagcctacc gcgtggctgg tttcaacctg
     8941 aatacggcaa cctggaaata atggaaaaga catacaaccc acaagatatc gaacagccgc
     9001 tttacgagca ctgggaaaag cagggctact ttaagcctaa tggcgatgaa agccaggaaa
     9061 gtttctgcat catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcacgcct
     9121 tccagcaaac catcatggat accatgatcc gctatcagcg catgcagggc aaaaacaccc
     9181 tgtggcaggt cggtactgac cacgccggga tcgctaccca gatggtcgtt gagcgcaaga
     9241 ttgccgcaga agaaggtaaa acccgtcacg actacggccg cgaagctttc atcgacaaaa
     9301 tctgggaatg gaaagcggaa tctggcggca ccattacccg tcagatgcgc cgtctcggca
     9361 actccgtcga ctgggagcgt gaacgcttca ccatggacga aggcctgtcc aatgcggtga
     9421 aagaagtttt cgttcgtctg tataaagaag acctgattta ccgtggcaag cgcctggtaa
     9481 actgggaccc gaaactgcgt accgctattt ctgacctgga agtggaaaac cgcgaatcga
     9541 aaggttcgat gtggcacatc cgctatccgc tggctgacgg cgcgaaaacc gcagacggta
     9601 aagattatct ggtggttgcg accactcgcc cggaaaccct gctgggcgat accggtgtag
     9661 ctgttaaccc ggaagatccg cgttacaaag atctgattgg caaatatgtc attctgccgc
     9721 tggttaaccg tcgtattccg atcgttggcg acgaacacgc cgacatggaa aaaggcaccg
     9781 gctgcgtgaa gatcacaccg gcgcacgact ttaacgacta tgaagtcggt aaacgtcacg
     9841 ccctgccgat gatcaacatc ctgacctttg acggtgatat ccgtgaaagc gcccaggtgt
     9901 tcgataccaa aggtaacgaa tctgacgttt attccagcga gatcccggca gagttccaga
     9961 aactcgagcg ttttgctgca cgtaaagcag tcgttgctgc ggttgatgca ctcggcctgc
    10021 tggaagaaat taaaccgcac gacctgaccg ttccttacgg cgaccgtggc ggcgtggtta
    10081 tcgaaccgat gctgaccgac cagtggtacg tgcgtgccga tgtactggcg aaaccggcag
    10141 ttgaagcggt tgagaacggc gacattcagt tcgtaccgaa gcagtacgaa aacatgtact
    10201 tctcctggat gcgcgatatt caggactggt gtatctctcg tcagctgtgg tggggtcacc
    10261 gtatcccggc atggtatgac gaagcgggta atgtttatgt tggccgcaac gaagaagaag
    10321 tgcgcaagga aaataacctc ggtgccaacg ttgccctgcg tcaggacgaa gacgttctcg
    10381 acacctggtt ctcttctgca ctgtggacct tctctaccct tggctggccg gaaaataccg
    10441 acgccctgcg tcagttccac ccaaccagcg tgatggtatc tggtttcgac atcatcttct
    10501 tctggattgc ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaaac
    10561 cgcaggtgcc gttccacact gtttacatga ccggtctgat tcgtgacgac gaaggtcaga
    10621 agatgtccaa atccaagggt aacgttattg acccgctgga tatggttgac ggtatttcgc
    10681 tgccagaact gctggaaaaa cgcaccggca atatgatgca gccgcagctg gcggacaaaa
    10741 tccgtaagcg caccgagaaa cagttcccga acggcattga gccgcacggc accgatgccc
    10801 tgcgcttcac cctggcggcg ctggcgtcta ccggtcgtga catcaactgg gatatgaagc
    10861 gtctggaagg ctaccgtaac ttctgtaaca agctgtggaa cgccagccgc tttgtgctga
    10921 tgaacacaga aggtcaggat tgcggcttca acggcggcga aatgacgcta tcgctggcgg
    10981 accgctggat tctggcggag ttcaaccaga ctatcaaagc gtaccgcgaa gcgctggaca
    11041 gcttccgctt cgatatcgcc gcaggcattt tgtatgagtt cacctggaac cagttctgcg
    11101 actggtatct ggagctgacc aagccggtaa tgaacggtgg aacagaagcc gaactgcgcg
    11161 gtactcgcca tacgctggtg actgtactgg aaggtctgct gcgcctcgcg catccgatca
    11221 ttccgttcat caccgaaacc atctggcagc gtgtgaaagt actttgcggt atcactgccg
    11281 acaccatcat gctgcagccg ttcccgcagt atgatgcatc tcaggttgat gaagccgcac
    11341 tggccgacac cgagtggctg aaacaggcga tcgttgctgt acgtaacatc cgtgcagaaa
    11401 tgaacatcgc gccgggcaaa ccgctggagc tgctgctgcg tggttgcagc gcggatgcag
    11461 aacgtcgcgt aaatgaaaac cgtggcttcc tgcaaacgct ggcgcgtctg gaaagcatca
    11521 ccgtgctgcc tgccgatgac aaaggtccgg tttccgttac caagatcatc gacggcgcag
    11581 agctgctgat cccgatggct ggcctcatca acaaagaaga tgagctggcg cgcctggcga
    11641 aagaagtggc gaagatcgaa ggtgaaatca gccgcatcga gaacaagctg gcaaatgaag
    11701 gctttgtcgc ccgcgcaccg gaagcggtta tcgcgaaaga gcgtgagaag ctggaaggtt
    11761 atgcagaagc gaaagccaag ctgattgaac agcaggctgt tatcgccgca ctgtaatcaa
    11821 cacagtgatg aaaacgaagg ccggagcatg ctccggcctt ttttatctct tacagaaatg
    11881 gtaaagaagg cataactccc cgagaaatac tggctaagaa gcctttatct ggttgttctt
    11941 ctttattgat taaaggaagg tcatccaaat cgcccagcaa atacgtattt tttgcctgcc
    12001 agtcaatcat ccatattttc agcagtgggt aagccagacc acccgtaatc ccggatatca
    12061 ccaccaacgc acacatgcga taaagcatac cgtggtacgt taaagttgag cgaaaacgaa
    12121 tacgcccatc attcagtgac aggttgctca taaaatgatt tcgcaacgac accgttaaat
    12181 aactggtgct aacagcaatc ccaaaataat aaattaactg cgcgattatc attttacgtt
    12241 gcatttccat aaattgctgt aaattctcaa tatcatcatt tgcataccca gaactatcat
    12301 actcatttaa tatttgatcg aagataatat aaccagcaac agcgagaaaa ggtaataaag
    12361 ctaaaaatgc gaggatggca tattttatac aatacgcagt atctaatttc accttgaaac
    12421 gatgtatacc gaaactggta ttgctccaga gaaaactcat taccaggcta tataaagtac
    12481 cattaaaaat accaatggaa acaataccaa ctattgccat cagaaccaca gatataataa
    12541 cactacttga actattggca tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc
    12601 caatggccat taaaatcggc aagaaaaagg ttacccacca gaaccctttc aaagagcaat
    12661 taaaactaaa tcttacgccg ttgagactgg tcattaaggc ctgataacgc agtccttttg
    12721 ctgccataaa tataagcaaa accaacagta acaaagtcaa aacacagcca acaagcggca
    12781 tatctgctga cactgtcatt aagattgcga aatagaaaaa aacaaaaaca agacaactaa
    12841 caaaaacatt cccaccggta attccataag aaaatcgttg tccgttaact tccatattag
    12901 cataaagata acgcttacat ttcattaatg cccatggtaa atagatacct agcgttataa
    12961 tcgttaacaa cacattcacc acacaaataa gaaaatatct ctcacctgta ccatgaaaaa
    13021 caaaagaatg ggacggaaca tccatttcat taataacttg agccataaaa atcccctgaa
    13081 ttaaaaaatc cattgatata atttatagat acagacagta aaatgttctg ccacgttaca
    13141 atactcttat cagtaatagg ctggcaaaaa ctttattgct ttataagacg cttgcgcacg
    13201 taagaagaga gtggtattaa acaaatgtat aattacttta tttttattgg gttaaactat
    13261 gaataacatt gcgccgcaat cacctgtaat gcgtcgcctg acgctgcagg ataatcctgc
    13321 tatcgccagc gtcatccgtc aggtttacgc agagtatggt cttaccgccg ataaaggcta
    13381 caccgttgcc gatccgaatc tcgacgaact gtatcaagtg tacagccagc ctggccatgc
    13441 ttattgggtg gttgaataca atggcgaagt ggtcggcggc ggcgggattg cgccattaac
    13501 aggtagtgaa gcggacattt gcgaactgca gaaaatgtac tttcttcccg ctattcgcgg
    13561 taaagggcta gcgaaaaaac tggccttaat ggcgatggag caggcacgag agatgggctt
    13621 caaacgctgc tatctggaaa cgaccgcttt tttaaaggaa gccattgcgc tttatgagca
    13681 tttgggcttt gagcatatcg actatgcgct tggctgcacg ggccatgtcg attgtgaagt
    13741 gcggatgcta cgcgaactct aaaaaaatgc ccgctagtat gtgctatcgg gcattttaat
    13801 caattaatta gtggcgaacc ccgtcatcgt cttcatcgac aaaatcttca tcgtcgccat
    13861 cttcgccgtt aggatcttca aagtaagtgc cccaaccgtc gtactcaacg tcaaatttct
    13921 ccgccagcgt catcagttgt tcaacctggg catcgatcag atcggcattc aacgcgcact
    13981 cgctgaggat gtcgcagcaa atcacgatat caccatcttc aacttccagc tcttctggat
    14041 cggtcacttc gtaaccgagt ttaaacgctt caactgccgc tttttccaga gtttccagat
    14101 cgtctgcgga aagatggtgt tcgatggtgt acagcgcgtc cggatcgctg ccatcttcca
    14161 gtaattcttc aataatcaaa cgtgtttctt cacgctgttc ttccagttgt tccgggtttg
    14221 ccatggctca ttcctcttaa tgtcctgccg atactcttat tgtcacacac cactgacatt
    14281 gcctccacct ttgtgacaaa gatttatgct ttagacttgc aaatgaataa taatacacat
    14341 aaattgagtt ttaattcatt gaggcgttag ccacaggagg gatctatgtc cgggttttat
    14401 cataagcatt tcctgaaatt actcgatttc acgccagctg aactcaacag cctgctgcag
    14461 ttagccgcga agctgaaagc tgataagaaa agcggtaaag aagaagccaa actcactggt
    14521 aaaaacatcg cgctcatctt cgaaaaagac tcgactcgta cccgatgctc tttcgaagtt
    14581 gccgcatatg accagggggc tcgcgttact tatcttggcc caagcggtag tcagattggt
    14641 cataaagagt cgattaaaga cactgcccgc gtgcttggtc gcatgtatga cggtattcag
    14701 tatcgcggct atggtcagga gattgtcgaa acactggcgg aatacgctgg cgtgccggta
    14761 tggaacggcc tgaccaatga gtttcacccc acgcagttgc tggcggatct cctcaccatg
    14821 caggagcatt tacccggtaa agcattcaac gaaatgacgc tggtctatgc aggtgacgca
    14881 cgtaacaaca tgggcaattc gatgctcgaa gctgcggcgc ttaccg
//
LOCUS       DAJTYM010000093        14470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTYM010000093 DAJTYM010000000
VERSION     DAJTYM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(104..370)
                     /locus_tag="OO575_003059"
     CDS             complement(104..370)
                     /locus_tag="OO575_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6665937.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            644..4168
                     /gene="nifJ"
                     /locus_tag="OO575_003060"
     CDS             644..4168
                     /gene="nifJ"
                     /locus_tag="OO575_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6665938.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4535..5664
                     /gene="ompN"
                     /locus_tag="OO575_003061"
                     /pseudo
     CDS             4535..5664
                     /gene="ompN"
                     /locus_tag="OO575_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5805..6239
                     /gene="uspF"
                     /locus_tag="OO575_003062"
     CDS             5805..6239
                     /gene="uspF"
                     /locus_tag="OO575_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6665939.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6415..7350
                     /gene="ttcA"
                     /locus_tag="OO575_003063"
     CDS             6415..7350
                     /gene="ttcA"
                     /locus_tag="OO575_003063"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6665940.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7479..8846)
                     /gene="dbpA"
                     /locus_tag="OO575_003064"
     CDS             complement(7479..8846)
                     /gene="dbpA"
                     /locus_tag="OO575_003064"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6665941.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9324..10307)
                     /gene="zntB"
                     /locus_tag="OO575_003065"
     CDS             complement(9324..10307)
                     /gene="zntB"
                     /locus_tag="OO575_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6665942.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10562..11794
                     /gene="dgcM"
                     /locus_tag="OO575_003066"
                     /pseudo
     CDS             10562..11794
                     /gene="dgcM"
                     /locus_tag="OO575_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11815..12378)
                     /gene="smrA"
                     /locus_tag="OO575_003067"
                     /pseudo
     CDS             complement(11815..12378)
                     /gene="smrA"
                     /locus_tag="OO575_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12632..14086)
                     /locus_tag="OO575_003068"
     CDS             complement(12632..14086)
                     /locus_tag="OO575_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6665943.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14083..>14470)
                     /locus_tag="OO575_003069"
     CDS             complement(14083..>14470)
                     /locus_tag="OO575_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6665944.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3856 a         3648 c         3418 g         3548 t
ORIGIN      
        1 gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta acgctggcga
       61 ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag ctgccacagc
      121 tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca cacatcccaa
      181 tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt gcacaatttg
      241 cttctccact actggacatc gacgctttta aacttggcgc tacgtgcgcc gcagtcgcct
      301 gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct acccaaaacg
      361 ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat aaataatagg
      421 cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt tactttttta
      481 tggctatttt tttgccctct gtttgatcaa aacattcatt actctgatgt gggggacaca
      541 aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa ggattactac tgggcgcgca
      601 gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta ctattgacgg
      661 taatggcgcg gttgcttcgg tgacgtttcg catcagtgaa gttatcgcca tctaccctat
      721 tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg gcttgaagaa
      781 cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg ctatcgctac
      841 cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat cgcagggttt
      901 gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctgacgccat ttgtcctgca
      961 tgtagcggca cgtaccgttg ccacacatgc actttctatt tttggcgatc attccgacgt
     1021 tatggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gctaacgtcc aggaagcgca
     1081 agactttgct ctcatttcgc acatcgcaac gctgaagagc cgcgtgccat ttattcattt
     1141 ctttgatggt tttcgcacgt cccacgaaat caataaaatt gtcccgctgg ccgatgacac
     1201 tattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg cgctcaaccc
     1261 ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc agtctcgcga
     1321 agccaccaat ccctggtaca acgcggtcta tgaccatgtt gaacaggcga tgaatgattt
     1381 cgctgccgcg acaggtcgtc agtatcagcc gtttgagtat tacgggcatc cgcaagcgga
     1441 acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg ttgatgaatt
     1501 gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgt ctgtaccgtc ccttctccgc
     1561 caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac tggacagaac
     1621 caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtgatgaccg cactggcaga
     1681 agcctttaat aatggtgagc gcgaaaccct gccccgtgtc attggtgggc gctatggtct
     1741 ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc tcaacgcggc
     1801 taaacctaaa gcacgcttta cggttggtat ttacgatgat gtgaccaatc tgtcactgcc
     1861 gttgccggaa aacactctgc caaactcggc gaaacttgaa gccttgtttt atggccttgg
     1921 tagtgatggc agcgtttccg caaccaaaaa caatatcaag attatcggta attccacgcc
     1981 gtggtacgca cagggctatt ttgtttacga ctccaaaaaa gcgggcggcc tgacggtttc
     2041 tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc aggctgattt
     2101 tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc gtttaaaacc
     2161 tagcggcatt ttcctgctta atacgccgta cagcgcagat gaagtgtggt cgcgcttgcc
     2221 gcaggaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga ttaacgcggc
     2281 gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc agatggcttt
     2341 tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc agggtgcgat
     2401 tgccaaaagt tacagtagca aaggtcagga tctggtggag cgcaactggc aggctctggc
     2461 gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc acagcgccaa
     2521 tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa ccgcggcgat
     2581 gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg gcacctggcc
     2641 gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca tctggaaaga
     2701 ggaactctgt acccaatgta accactgcgt cgccgcttgc ccacactcag ctattcgcgc
     2761 aaaagtggta ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt cgctagatgt
     2821 gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggcaccgg aagattgcac
     2881 cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc cagagattaa
     2941 agccatcaat atgatgtctc gccaggaaca tgtcgaagaa gagaaaatca attacgattt
     3001 cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata ttcgtacatc
     3061 gcagctgatt acaccgctgt ttgaatattc aggtgcttgc cccggttgtg gcgagacgcc
     3121 gtatattaaa ttgctgactc agctctatgg tgaccggatg ttgatcgcta acgccaccgg
     3181 ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg atgccaacgg
     3241 tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg gccttggttt
     3301 ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc aatttgccga
     3361 taaaatcccg gcggaattac tgacggcgtt gaaatcagac gctacgccag aggttcgtcg
     3421 agaacaggtc gcagctttac gccagcaact caacgatgtt gccgaagcac atgaacttct
     3481 gcgtgacgca gatgcactgg tggaaaaatc aatctggctg attggtggcg acggctgggc
     3541 ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa acgtcaacat
     3601 tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga aagcgacacc
     3661 gctgggtgca gtaactaagt tcggcgagca cggcaaacgt aaagcgcgta aagatcttgg
     3721 cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg gcgcgcagct
     3781 gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat cgctgatcat
     3841 tgcttatagc ccgtgtgaag agcatggtta cgatctggct ctcagccacg accagatgcg
     3901 ccaactcact gctaccggct tctggccgct atatcgcttt gatccgcgtc gtgccgatga
     3961 aggcaaactg ccgctggcgc tggattcacg cccgccgtca gaagcactgg aagaaacgtt
     4021 acttcacgag cagcgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg cagaacagtt
     4081 atggaaagat gctgcagcta atttgcaaaa acgctatgac ttcctagcac aaatggccgg
     4141 aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc aattctaaaa
     4201 aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc agtggcaatg
     4261 caatggccca acagaaaata aaatctgtga caataaaggc atataacccg cgcagaataa
     4321 cgtataggaa attaaaagta taatttttat tttgtatgta ttatcttcga tacattcacc
     4381 agacttatta tcatttcaga ggaattattt gattaaggtt ttacttaagg cgtaacaaat
     4441 gatatttatc ggctaactga acttctcctt taggatgttt tcactccccg aaatggggat
     4501 aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agaaaagtac tggcactttt
     4561 aattcctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata ataaagacgg
     4621 caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg ataattcagc
     4681 gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc aaattaacga
     4741 tcaacttact ggctacggtc aatgggaata caatattcag gcaaacaaca ctgaatcttc
     4801 aaaaaaccag tcatggaccc gtctggcatt tgccggactg aaatttgcag attacggttc
     4861 tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga ccgatatgct
     4921 gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg gtcgagccaa
     4981 tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc tgaacttcgc
     5041 ggtgcagtat caaggtaaca acgaaggagc cagtaatggt caggaaggca ccaataacga
     5101 acgcgatgtt cgtcatgaaa acggtgacgg ctggggtctt tccacaacat atgatttagg
     5161 catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg accaggttaa
     5221 ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa aatacgatgc
     5281 taacaatatt tacctagcaa ccatgtattc agaaacgcgt aatatgaccc cgtttggcga
     5341 cagcgattat gcagttgcga acaaaaccca gaactttgaa gttactagta ccagtttgat
     5401 ttttgtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct tcacgctgct
     5461 ggtggtgcag acaacccggc aggtgttgat gataaagatc tggttaaata cgccgatgtt
     5521 ggcgcgactt actatttcaa taaaaacatg tccacctatg ttgactataa aatcaacctg
     5581 ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga tattgtcgct
     5641 ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg ctttttctgc
     5701 ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca caaaaagtgt
     5761 catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac agaacgattc
     5821 ttgtccctat cgatatttca gattcagaat taactcaacg cgtgattagc cacgttgagg
     5881 cagaggcaaa gattgatgat gcagaggttc atttcctgac ggtaatacct tcactgccct
     5941 actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat gacctgaaag
     6001 cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca accgacagag
     6061 tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat tctggaattg gcgaagaaga
     6121 tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact tatctgctcg
     6181 gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt gtgcgctgac
     6241 actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg ctgacatttt
     6301 cccctctaat ccgtaccata cacgccacag tttttatatc agatttctta tgctgagcgt
     6361 tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg
     6421 caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaaattaca aaaacgtctg
     6481 cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg cgatcgcatc
     6541 atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct gcgcaatttg
     6601 cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca aaagcaaccg
     6661 ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga gtacaagatt
     6721 gttgaagaga acacttacgg tatcgtgaaa gagaagattc cagagggcaa aaccacttgc
     6781 tcactgtgtt ctcggctacg tcgcggtatc ctttatcgca ccgcaacgga actggggacg
     6841 acgaagatcg cgttgggtca ccatcgtgac gatatcctgc aaacgttgtt cttaaatatg
     6901 ttctacggcg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga tggcaaacat
     6961 atcgttattc gtccactggc ctactgccgc gagaaagata ttcagcgatt tgccgatgca
     7021 aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct gcaacgtcag
     7081 gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat cgagacgatg
     7141 ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct gttcgatttc
     7201 aaaggcatta cccacggttc tgaagtggtt aacgggggtg atctggcgtt tgatcgcgaa
     7261 gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca gttggatgag
     7321 ttacggctga atgtggttga agtgaaataa ccaggatagc gcccgatgcg caagcgtatc
     7381 gggctactct tatggaggcc ggataagacg cggccagcgt cgcatctggc aatcccgaat
     7441 aagatgttta ctcttgcacc cggcaattca acatttcatt attttaataa ccgcacccgg
     7501 cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg agcaacagcc
     7561 tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat atctgcgcca
     7621 tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat tttggctttt
     7681 ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcacaat ggaactatta
     7741 gctggcggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat attggcccgc
     7801 tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc acgagctgta
     7861 cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt caccaccagc
     7921 tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcaaccag tacacgggcg
     7981 ctaccgttag caaaacgtac cagggtctga tcgcgttgct ccagatcgcc gtgtaacgac
     8041 aatgcacttt gctctacttc attcagcgcg tcgcagacag cctggcaatc ttttttggta
     8101 ttgcaaaaca ccacacaaga ggatggctga tgcaagctta ataaccgttg caacagagga
     8161 attttgcctt tactggatgt ctcataaaat tgttgttcaa tgggtggcaa agcatctgtt
     8221 gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattgc ggcgatggct
     8281 tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa acggatgaca
     8341 tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc catcaccaaa
     8401 gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa acgacccggc
     8461 gttgccacga taatatgcgg cgcatgttgc agcgaatcac gctgcatacc gaacggttga
     8521 ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg acgcaattca
     8581 cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg ggtttgaaat
     8641 agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt gccgctgccg
     8701 gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc ggcggcctgc
     8761 accggcgtca tggttaaata acccaactca ttaaggttcg tgagttgggc gggaggcaaa
     8821 acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg caaagttagc
     8881 aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt tcttcatcgg
     8941 gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga acaggatcgc
     9001 caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata tttccctccg
     9061 gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa aaaagccgac
     9121 tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa aaaggaagta
     9181 agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa gggagatatt
     9241 gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt tttgtataca
     9301 gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca taaagcaaca
     9361 ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg ccacccgccg
     9421 ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact gggtaaaaag
     9481 accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg catcacctga
     9541 gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc gtcaaggccg
     9601 cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct catccacggc
     9661 aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata gcgacgcatc
     9721 acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg ctgatcaagg
     9781 agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc actggaatga
     9841 tcggtcaacg catcgcacac atccaccagc catcccccgc aatcggtcgg acccgtgccc
     9901 tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg ggtcgaaaca
     9961 attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg ttcatcggtg
    10021 ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgaaacggct gactcggggc
    10081 cgtgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg tgttgtcgcc
    10141 agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg atgcgcttca
    10201 tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag catccaggca
    10261 aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccactgt gcctccatca
    10321 tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct catcacccca
    10381 ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca aaatgctttt
    10441 ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat tatctgatca
    10501 tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata acgggatctc
    10561 aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct ggattgtttc
    10621 gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga tgcaagacct
    10681 cacgtttagt cagctacgaa ccggacccta ttccgtctcc tcacaaaaag aactgccgaa
    10741 atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg ttcagcgtaa
    10801 agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag aaacagaacc
    10861 ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag ccagtcgctc
    10921 ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga ctaactctgc
    10981 accaatgttg ttgattgacc cgtcacgaga tggacaaatc gtcgatgcta acctcgccgc
    11041 gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct aggaaataaa
    11101 tatgcttggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac ccggcggtca
    11161 taaccctttg aattttgttc ataaactggc ggatggatct actcgtcatg tgcagaccta
    11221 tgccggaccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc atgatattac
    11281 tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg cgatgaccgg
    11341 attactgaat cggcgacagt tttatcacat tacggaacca ggccaaatgc agcatctcgc
    11401 catcgctcag gattacagct tgttgctcat cgacaccgat cgttttaaac acattaacga
    11461 tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgcc ccctcgaaag
    11521 ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg tcttattgct
    11581 accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg taagcgtggc
    11641 aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg cgcatcacga
    11701 aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt atcgggcgaa
    11761 aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc gagatcaacg
    11821 actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt tacgtagtgc
    11881 gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg cctgaacatc
    11941 atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat gtgatttatc
    12001 ttcccgccct ttaccatgaa taatcagcac attacgcaaa ccatccgcca gggcttgttg
    12061 aatgaaactg aacaccattt tgcggcactc ttccaccggc tggcgcaaaa gattcaggct
    12121 cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgct acaacccttc
    12181 ctgccgaaac tccagcggct gacttagcgg gatgatgtcg agaaatccgg tagtgaggaa
    12241 attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg ttggatgcca
    12301 gtggacatct gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa gaaacagcga
    12361 tttgtcgtca aggttcatgt aacctcctat gcaccttcac acgtcgtgat tcagacgggt
    12421 taattgttca tacttactgc atcgttccct tcacagattg aaacaatact ctacttcata
    12481 cctgtgagta tctgcgcagg cggggcattt tatactagcc tgttagcagt atcgaacagt
    12541 ttagcccgag tttaattcag cacaggctcc gatcaggatg atttattcat agcaggaacc
    12601 aacaaaaatg cgataaataa cagaccactg attagcagaa aggtaccgaa aatgccatag
    12661 tttgcgcctc cctcagcatc aattgactca ataatttggt tgaagccagt atttccgatg
    12721 tctggtcact tctactgcga ttaaaaaata aagcacttct ggaaatgaag tcattcaccg
    12781 tatggggaac agaaaccatt ttttcaccca ttctcgaaaa atgaaaatta gttcgattat
    12841 tgagaataga gccacacagg gcaataccaa tcgcgccccc aagattacgt gttaaattaa
    12901 atacaccaga ggctaacttc agccgttctt ttggaattcc ccccagcgtt aatgttacga
    12961 tgggggccat cgcaaactgc tgcgaaatcc ctctaattgc ctgtggaaac agtaattcct
    13021 gccatcccca ttcgtgggtt ataggggtaa aaagatacat tgaaaacaca aatccgccca
    13081 gccccgccat caacagccat cgcagattta tttttttact taaccagaag taaaagggaa
    13141 cagaaaataa ctggaaaata cctgttgtgc aaacggcaaa acctatctct tcggcattta
    13201 atccgcgaat ctgccccaaa aacacaggta ttaaatatac agtagagaat atcccaaccc
    13261 ctccagaaaa agaaaaaaaa catcccagag tgaaattttt atccttaaaa gcatgaagat
    13321 ccattatcgg attggatatt gtaagtgtac gcgcagcaaa taaaataaaa ctcaccaatg
    13381 ccagaactga tgtcagcaaa atagtattat catccagcca tccccagcgc gctccttctt
    13441 caagggtata ttccagacat cccagagtca tggccaacag gataatactc ggataatcag
    13501 caactttcag taaagagaga tcaggtttat caaaattaac cagaaaagga atactcaaaa
    13561 ctaaatatat gccaggtaat atattgatat aaaatagcca tcgccagtcg aggttatcag
    13621 tgagccagcc ccccagagtt ggccctagcg tcggtgacag tgaggccagc gcactgacta
    13681 ccgcagcagc caaccccaac tctttaccct ggtaataaat aaacgccatc gtgaaaacta
    13741 aagggatcat ggatgcgcca gcagctccct gtaaagcacg aaacaaaatc atgatctgaa
    13801 tattccatgc aagtccacac gcgatactca ttaaagtaaa tattcccgcg gatagagtaa
    13861 aaagccaacg agtggaaaat acccgtgaca gccacccaga gagcggaata acaataatct
    13921 cagcaattaa atagctggtc tgaagccagg ccatttcgtc tttacctgcg gacagaccac
    13981 cacctatttc atttagggat gacgatacaa tttgaatatc aagctgagca ataaataatc
    14041 ccatacacat actggcgaaa gcaatatgtt tacgcataga catcatctct catcaacact
    14101 gacggtgacg gacagtcccg ggcgaagaag ttgctttaat tcctttgcgt cgtcaaatgc
    14161 aatccgtacc gggacacgct gaacaatttt ggtgaaattg ccagtggcat tttctatcgg
    14221 aatcaagctg aaactggctc ctgtcgctgg ggaaaggctt tcaatgtgac cgtgaaacac
    14281 ttcccctttg agaatatcag cacggatctc agccttcatt cctggcttca ttcccgctat
    14341 ttggttttct ttataatttg catcaaccca caactcactg actggtacta gtgaaactaa
    14401 cgatgtgccg ccttccaccc aactgcctgt atgggcgctg cggtttgcga caatgccatc
    14461 aatcggcgca
//
LOCUS       DAJTYM010000094        14028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTYM010000094 DAJTYM010000000
VERSION     DAJTYM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..377)
                     /gene="csgB"
                     /locus_tag="OO575_003070"
     CDS             complement(<1..377)
                     /gene="csgB"
                     /locus_tag="OO575_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6665945.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKAN"
     gene            1131..1781
                     /gene="csgD"
                     /locus_tag="OO575_003071"
     CDS             1131..1781
                     /gene="csgD"
                     /locus_tag="OO575_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6665946.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1786..2175
                     /gene="csgE"
                     /locus_tag="OO575_003072"
     CDS             1786..2175
                     /gene="csgE"
                     /locus_tag="OO575_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6665947.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2200..2616
                     /gene="csgF"
                     /locus_tag="OO575_003073"
     CDS             2200..2616
                     /gene="csgF"
                     /locus_tag="OO575_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6665948.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2643..3349
                     /locus_tag="OO575_003074"
                     /pseudo
     CDS             2643..3349
                     /locus_tag="OO575_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3482..3973)
                     /locus_tag="OO575_003075"
     CDS             complement(3482..3973)
                     /locus_tag="OO575_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6665949.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4075..4629)
                     /gene="ycdY"
                     /locus_tag="OO575_003076"
     CDS             complement(4075..4629)
                     /gene="ycdY"
                     /locus_tag="OO575_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6665950.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4653..5390)
                     /gene="ycdX"
                     /locus_tag="OO575_003077"
     CDS             complement(4653..5390)
                     /gene="ycdX"
                     /locus_tag="OO575_003077"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6665951.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5445..6383)
                     /gene="ghrA"
                     /locus_tag="OO575_003078"
                     /pseudo
     CDS             complement(5445..6383)
                     /gene="ghrA"
                     /locus_tag="OO575_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6618..6705
                     /locus_tag="OO575_003079"
     tRNA            6618..6705
                     /locus_tag="OO575_003079"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6652..6654,aa:Ser,seq:gga)
     gene            complement(6855..7136)
                     /locus_tag="OO575_003080"
                     /pseudo
     CDS             complement(6855..7136)
                     /locus_tag="OO575_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7418..8422)
                     /locus_tag="OO575_003081"
     CDS             complement(7418..8422)
                     /locus_tag="OO575_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6665952.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8494..9054)
                     /locus_tag="OO575_003082"
                     /pseudo
     CDS             complement(8494..9054)
                     /locus_tag="OO575_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9139..9336)
                     /locus_tag="OO575_003083"
     CDS             complement(9139..9336)
                     /locus_tag="OO575_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665953.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9348..9839)
                     /locus_tag="OO575_003084"
     CDS             complement(9348..9839)
                     /locus_tag="OO575_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665954.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(9836..10210)
                     /locus_tag="OO575_003085"
     CDS             complement(9836..10210)
                     /locus_tag="OO575_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6665955.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10257..10631)
                     /locus_tag="OO575_003086"
     CDS             complement(10257..10631)
                     /locus_tag="OO575_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6665956.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(10794..11015)
                     /locus_tag="OO575_003087"
     CDS             complement(10794..11015)
                     /locus_tag="OO575_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6665957.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11078..11554)
                     /locus_tag="OO575_003088"
     CDS             complement(11078..11554)
                     /locus_tag="OO575_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6665958.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11570..12043)
                     /locus_tag="OO575_003089"
     CDS             complement(11570..12043)
                     /locus_tag="OO575_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6665959.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12385..13206)
                     /locus_tag="OO575_003090"
     CDS             complement(12385..13206)
                     /locus_tag="OO575_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6665960.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13306..13479)
                     /locus_tag="OO575_003091"
     CDS             complement(13306..13479)
                     /locus_tag="OO575_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6665961.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13599..>14028)
                     /locus_tag="OO575_003092"
     CDS             complement(13599..>14028)
                     /locus_tag="OO575_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665962.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3585 a         3434 c         3364 g         3645 t
ORIGIN      
        1 atatttgctt tattaccaga acctttctgg ataatcatcg cagtattacc ataagcacct
       61 tgcgaaatac tggcatcgtt ggcactgccc gcctgatcaa tatatgcaag gttataatct
      121 cctgtctggt caatctttgc ccgattgcta ctaccttctt gcgcaacaac cgccaaaagt
      181 tttgagcctc cctgccgtaa ctgagcacta ttattagtcc cagcctgacc aattatggct
      241 gcctgattaa atgaagactt actcaattca tttaccgcga agttatattc tgaattagct
      301 aaatcataac ctgctgcggc tgcaatccca ggcgcaccca gtattgttaa catcataaat
      361 aacaatttgt ttttcatgtt gtcaccctgg acctggtcgt acatttaaga aattaaatca
      421 tttcaacttg gttgttaacg caacctgtat tttgttaacg ctgcgttacg atggaaagta
      481 tgtctgcgga aatattttta ataactcacc cgcgcgttgt atttttcttt ttttgttcat
      541 accaaagtat taaaacccgc gtaaatacgc tgttatctac gcaaaaaata ttttgttttc
      601 tcttaaatct ccgttttccg ctatcaaaaa tcaccagaca gtcattcttc ttgcccgtcg
      661 ctgattgctg cgatatgtct tgcgcacaag ccgtgacaaa cctcgtcttt ttgcagtaaa
      721 aaaattgtcc acggaggtgt ggagaaaaaa caaaaacgtt ttacatgacg aaaggactac
      781 accgaaatat tttttatatg tattattagt aagttagcac catttgtatg attttttaaa
      841 attgtgcaat aaaaaccaaa tgtacaactt ttctatcatt tctaaactta ataaaacctt
      901 aatgttaaca ttttaatata atgagttaca ttttgttaca tgtttaacac ttgatttaag
      961 atttgtaatg gctagattga aatcagatgt aatccattag ttttatattt tacccattta
     1021 gggctgattt attactacac acagcagtgc aacatctgtc agtacttctg gtgcttctat
     1081 tttagaggca gctgtcaggt gtgcgatcaa taaaaaagtg gggtttcatc atgtttaatg
     1141 aagtccatag tattcatggt catacattat tgttgatcac taaaccttct ttgcaggcga
     1201 cagctctctt gcagcacctt aaacaatcgc tagcaattac aggaaaatta cataatattc
     1261 aacgttctct ggacgatatc tcttcaggct ctattattct tctggatatg atggaagcgg
     1321 ataaaaagct tatccattat tggcaggata ctttgagcag gaaaaacaac aatatcaaaa
     1381 tattgttgct aaatacgcct gaagattacc cgtaccgcga cattgaaaac tggcctcata
     1441 tcaacggcgt tttttatgcc atggaggatc aagaacgtgt tgtcaatggg ttgcaaggcg
     1501 tcctgcgtgg cgaatgctac tttacgcaaa agcttgccag ttacctgatt acacattctg
     1561 gtaactatcg ttataacagc acggaatcag ccctccttac tcatcgggaa aaagagatcc
     1621 tgaataagct gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg
     1681 aaaatacggt taaaacgcat ctttataatc ttttcaagaa gatagccgta aaaaaccgga
     1741 cacaagcggt ttcctgggca aacgataacc tcaggcgata aagccatgaa acgttattta
     1801 cgctggattg tggcggcaga atttctgttc gccgcaggga atcttcacgc cgttgaggta
     1861 gaagtcccgg gattgttaac tgaccatact gtttcatcta ttggccatga tttttaccga
     1921 gcctttagtg ataaatggga aagtgactat acgggtaact taacgattaa tgaaaggccc
     1981 agtgcacgat ggggaagctg gatcacaata acggtcaatc aggatgttat tttccagact
     2041 tttttattcc cgttgaaaag agactccgag aaaactgtcg tctttgcact gattcaaact
     2101 gaagaagcac taaatcgtcg ccagataaat caggcgttat taagtacgga cgatttggcg
     2161 catgatgaat tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta
     2221 gttctactca tgcttatttc gccattaagt tgggctggaa ccatgacttt ccagttccgt
     2281 aatccaaact ttggtggtaa cccaaataat ggcgcttttt tattaaatag cgctcaggcc
     2341 caaaactctt ataaagatcc gagctataac gatgactttg gtattgaaac accctcagcg
     2401 ttagataact ttactcaggc catccagtca caaattttag gtgggctact gtcgaatatt
     2461 aataccggta aaccgggccg catggtgacc aacgattata ttgttgatat tgctaaccgc
     2521 gatggtcaat tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc gaccatccag
     2581 gtttcgggtt tacaaaataa ctcaaccgat ttttaagccc cagcttcata aggaaaataa
     2641 tcatgcagcg cttatttctt ttggttgccg tcatgttact gagcggatgc ttaaccgccc
     2701 cgcctaaaga agccgccaga ccgacattaa tgcctcgtgc tcagagctac aaagatttga
     2761 cccatctgcc agcgccgacg ggtaagatct ttgtttcggt atacaacatt caggacgaaa
     2821 ccgggcaatt taaaccctac ccggcaagta acttctccac tgctgttccg caaagcgcca
     2881 gggcttacaa aacctgctta acgagcgcaa ggttattcgt gcggcacaag aaaacggcac
     2941 ggttgccatt aataaccgaa tcccgctgca atctttaacg gcggcaaata ttatggttga
     3001 aggttcgatt atcggttatg aaagcaacgt taaatcgggc ggtgttgggg caagatattt
     3061 tgggatcggt gcagacacgc aataccagct cgatcagatt gccgtgaacc tgcgtgtcgt
     3121 caatgtgagt accggtgaga tcctctcctc ggtgaacacc agtaagacga tactttccta
     3181 tgaagttcag gccggggttt tccgctttat tgactaccag cgcttgctgg aaggggaagt
     3241 gggttacacc tcaaacgaac ccgttatgct gtgtctgatg tcggctatcg aaacaggggt
     3301 cattttcctg attaatgatg gtatcgaccg tggtctgtgg gatttgtaaa ataaagcaga
     3361 acggcagaat gacattctgg tgaaataccg ccatatgtcg gttccaccgg aatcctgaca
     3421 gataaaaatg ccggaattaa taatccggca tttactgttt aagcgaaagc gtgagtgccc
     3481 ctcacgcttt atttttgatt tcctgctcac ggtgagccgt ctttgcacta cgcgccgcca
     3541 gccacaggcc gctgtttttc attgcgtaac caaataccag cccaagcgcc agcgaaggta
     3601 acaccagttt ccagtcaccc tgcccggcaa acgtcgcgca tgcgcctata aacgtccccg
     3661 gaacaaatga aagcagcagc tgtttggcct gaatacacat cagaaacgcg acaataccgg
     3721 ttatgacata accgagaatt tccagatgtg gtgccagtgc actaccgtaa ataatgacca
     3781 tcgcccacac cacgccacta agcagggttg cagcggagat cgccagccct ttcagcccac
     3841 cttgcgggca agcaaagtag gccgtacagc ccaggaagcc cgcccagctt agtaagccca
     3901 gggaaacagc cacccatccc cagataccgg agagaatgcc tgttgtgatt gcaatagaga
     3961 gaagtatgtt catgccgcgc atgatagcag aaaacgcggc catgaatgag atcgagcaca
     4021 cattttatgc aacaacaaat tatctgttgc agttatgagt gacgatgatc acatttactc
     4081 ttcagaatct tcttccagct cgtcccacat tgcactaatg gcatcacggg ttagcggtgc
     4141 catggtgcgc cagaaaggcg tggttgcatg ggcctccact ttgccgagga acgcaccaca
     4201 ccagggtaac agatactcac tgaacagtgt ttccagtgct tcgctctcat cttccgttga
     4261 ctgatcttcc agccaggaag ccgcaagcag caatgtgccg atgtgatcgg ctggcgtatc
     4321 cgccagtagc atccctcgtt cggaaagaaa agcgcgcact tccgcttccg ttgcgtcctc
     4381 aacccatgcg ctacgatatg gcggcacggc acattcatcg ccgataaaca acgcattgta
     4441 atcggcagag acttgcgtca tatcacagct tttctgtaaa cgcgtcagta actcatcctg
     4501 ctccagtggc cagttcgcag ccagtttccc ctcacgaatc agagtaaaca gcggcaccag
     4561 taaaggatct tgcggttggc ggtaatagag cgaacccagc acacgacaga ggatagaaaa
     4621 ctcgttcatt aatatatttc cattactagt aattaaaggt ctgcaaattc cgcaatcggt
     4681 gccataccgc gagattcaag gaagttcagt aagcggcgcg gagaaacatt caaaatgcgc
     4741 tctagcggaa aatctaccgc gtcgaggatt ttaagacact cttcaaattc ccccatggta
     4801 aacgcggtgt gagaatccga gcctaatgcc acccaacctc cagcatcacg taccgctgca
     4861 gctaccgcac gacagttgtc ttcactgccc ttacgtgagt gtaaaaatga ggaattattg
     4921 atttccagcg ccacctgatg tttcgcggct gcttcagcaa cggctttaac atctatttca
     4981 tatttcggat ttccgggatg gcttattata tgcacattgc cgctggcgat agtggagatc
     5041 atcgcttgtg tattggtcgc tttgtcatgt ggcgcaaaaa ccggctcatg aaaaccggca
     5101 ataatcagat ccagcgagtc aaacatttta ccgctgcagt caatttcacc atcaacattt
     5161 ttaatgttag cttcaatgcc gcgcaggatc cctaccccat caaccactcg cggccagata
     5221 cgcatgttaa tgaagtgcca gtgatgcggc gcatcttcca tatccgggcc atgatcggtg
     5281 atcgcaaaaa gtttaatgcc cttttgtttg gcctgggcaa tgtaatcact caatgtgcta
     5341 tatgcatgtg tgctggcaac ggtatgcata tgaaggtcga cgggatacat aactctctcc
     5401 tgtattcaat tcccgccaag gatagcagga atcctgatgc tttattagta gccacgtgcg
     5461 cggtcgactt gcccgcagac cctctcccct ttttcgagct gggcaatagt gcgagaaatg
     5521 tactccacag cttcagcggg acgggtaatc gcggcgacat gtggtgttat cgtcacgcgt
     5581 ggatgttgcc agagcggact ttcaggcggt aagggttcac gattaaaaac atccagcatt
     5641 gcgcctttaa ctttcccgct atccagcgcc gcgagcaggt catcttccac aacatgaaca
     5701 ccacgcgcca ggttgaggag atacgcgcca tcaggtaatt tttcgagtaa ttgttgatta
     5761 ataatgccga cggtttcagg ggtattcggt aacaaattaa tcaatacccg acattggctc
     5821 agaaatgcag acagttcttc ccgtccggca aagctttgca cgccaggcca cgatttacgg
     5881 gttcgactcc agcaacgcag cggaaagcgc taggtttgca gactctgagc aactttacag
     5941 cccagtacgc ctgcgcccaa aatgccgatg gtaaaatctt cccgatgata ttcaggcagc
     6001 ggttgccaat gcgaactatt ttgctggatg cgataatcgt caaaacgtcg aaaccaatgc
     6061 agcacctgac tgacagcata ttcctgcatt tgctcgccca taccggtatc ttccaggcga
     6121 aaaagtggaa cagaagggtt cagcatttca gggtgtgcct gtagcttgct caaaatagaa
     6181 tcaacaccgg ccccgagtgc gaacaccgct ttaagatcgc gccctgccag catttcaaca
     6241 ggaggatgcc agactaaaac ataatcagca gagtcattat ctccgctttt ccatgccctg
     6301 actcttgcct gaggaatagc tttgcgcagt gcctcaatcc accattgggt atcgaacgtt
     6361 gggtgataaa agatgatatc catactgact cccgaaaagc gttgtgcgaa atttattcgc
     6421 acttatcgtt atgatctaca aaggccacca gcataacaaa tccgtggtcg gtggcaaaaa
     6481 aagcagattt cgcttattaa aaccacacat tgattgaatg tttgaataaa cgcgcgattt
     6541 tttcaaaaag tttgttgacc tcaggtcatg atttccctaa attagcgccc gttccagcaa
     6601 gacaggaacg acaatttggt gaggtgtccg agtggctgaa ggagcacgcc tggaaagtgt
     6661 gtatacggca acgtatcggg ggttcgaatc cccccctcac cgccagatta taagtgcatg
     6721 ttattgttta acatgcgctt ttttattttt ccttccagac agtatgccat cgggtatgcc
     6781 atctgaaacc ggtttatatg ccccgcgatc atatcttatc ggacaatttc cgatctttga
     6841 gcatactggc aaagctatac tgccagcata tcaggatagt gttttgcgat aatatcattc
     6901 agtctgtcaa ccagttctgg ttttctgaac gtgatatgcg cggagccctt cttaaagtat
     6961 ctgatgctga acatctcatc ttcatagctc tctttaccct gaacagactg aatgtgatca
     7021 tccagacgaa gggtgatgtt tccccggttg tcggggattg gtttaccgct gaacaggtgc
     7081 aacattcttt ccagatcggc gagtcggtca gcctgttgtc cggtgataag atgaaatcta
     7141 tggtcacccc cgtttttgca acactgattt ttgattaatg gttggcttgc ttaaatctat
     7201 ccggcgtctc tgtgggcaag gatgcccgcg ccccgatgag ttccgcacct gataatccta
     7261 aaaatcccct cggcttcagt gagccatttc atctgttagg tttttaatca ggtcgatttg
     7321 ccgttcatgt catcaataat cagtcttcgc aaaaccagaa aggtaacttg tatcttattg
     7381 tttaaaataa actaattgag ttatatcagc atctatatca ggcctgatat tcttttttct
     7441 gagtagtgat tgcccaggca attcgagcca gtttatttgc cagagcgcag gtaaccacat
     7501 tcgaatgctt cttgttgagt tgctcccgaa cccactcagc aagttttcca tgcttgcgtt
     7561 caagtcgcat tatgaatgag cgggcacact gaaccagtaa ccggcgtaaa tttttatcac
     7621 ctcgcttact gatacccaac agagtcgatt ttcctccagt gctatactgc cggggaacaa
     7681 gtcctgtgga agctgcaaaa tccctgctgc atgagaactg tttcccgtca ccaagttgcg
     7741 acgatagtaa gctggcagta atcggaccaa caccaggaat tgtcataatg cgctgagcgg
     7801 tatcatcagc gttgattgac tgacttaatt ctgattccag ctctgctatt tgctcaacaa
     7861 ggtaaaagta gtgggtatgc aacctcacca aaaggcggct cagatattcg gggatttcgt
     7921 gttcagccaa cacgagagac aaccgtttta ttacagcatc acctatcggc aggctgattc
     7981 caaattccag caagaaaccg tggatctggt tggttgtttt caccttatct ctgatgagcg
     8041 attctctgac ccggtgtaac gccctcatgg cctgctgggt ctcagttctt ggctggacaa
     8101 aacgcattga tggtcgtgaa gccgcttcgc atatggcttc tgcatcaaca aaatcattct
     8161 tattactttt gacaaacggg cgaacgaact gtggagaaat cagcttagtt tcatgcccaa
     8221 aatcagagat acagcgagcc ataaagtgtg cacccgcaca ggcttccatt acaacaactg
     8281 agggcggaca tgtcgccaga aactgcatca gtttagtacg ggtaaatttt ttacgcagaa
     8341 gagccttacc agacttgtct tgacaatgaa tgtggaagga gtgctttccg agatcgatac
     8401 caataagtgt gatgttctgc atgatggtct ctccgttaaa gggatttacc ttaacagcgt
     8461 actgctgtta aggtgggggt gaccatatca ttatccccac cggtcccatc tcaccagatt
     8521 gttgacgata attttactgc caaatttaca ggggcaattg gttttgtaat tccagttcag
     8581 ccctctgaag acgttgatca cccctcgctc aaacacctca tccttattct ggtgcagttg
     8641 ttcaaatgtg ctcagaatgt tcgcttcgct gatttccgga aaattatcgt actccagtga
     8701 ccggtaccat gtatcacggg cctgcgtatc cattaatgac agcatcccgg aacgttgcat
     8761 caggtcacgc cagatttcgc gatcaagatt ccgggtaatg gctttcattg ctgtttcggg
     8821 tttctccatc agccagcagc cgcagcgaaa atcctgtttc atggcccagt ccggggcagt
     8881 tttacctccg atgctttccg tcagcaccga gatttcagcc agttgtcgga taagtgtgtc
     8941 gatttgcaac agtgtactct ttcgtacagc cagaatgtgg cgaatgctgg ttgagcagat
     9001 aacgtccgta tgcccgttga ccacttccgg acgggagatt gtgatatctg acataatcga
     9061 tattcctttt tgcagataaa tgaaaacgcc agccctgtgc aggctggcgt cagaggaatg
     9121 aagtctggtg agtgagcttc accggttctg gtgcagaagc tgcagatgac gcagggtacg
     9181 gacatcctcc cgggcctgtt cgatacaggg caacagtgct gctgaatccg ttttatcctc
     9241 atcgttgtca aagataattc ccgatccgca gtcgatattg tcctgcagcc acgcaatcag
     9301 aatatccagc gctgtttccg tggttaatga tttcatgttg ttcctggtta ccagtcgaaa
     9361 gtgggtaaac ccggcaggca ttcagcgctg gcatccagat gaatgagact gacgccataa
     9421 cgccggatga gtgtgacgac cagacgacgg aacgtttttg acagccccag gcgtttaagc
     9481 cgcagaaccg ggtatgacca cgcatcggta cgtaacagat atccgctacc tgtaaaatga
     9541 atccattctg attcaccgaa gtcactggtc tggtgtgaca gcgagtacaa ccaggcgttg
     9601 tccgtttccg tgatatgtgc ggtactgcag cgtatgccgg taagggtagc aaatggtggc
     9661 gtgcattctg ctgaggtctg gtcagattca tcaacaacac gaagggagta gccgttatcg
     9721 cagaccatgt taatcagttc agcgagctca ataccatcga cgtcaacgac aatgcgccct
     9781 atgttcagtg cctgtacgtt aacgctgtcg gcttccagtg tcagggccag tttcatcatt
     9841 tcgcctccgg atacttaccc agggtaatgt tatttaccgt tctgtaattg tcgcgggtca
     9901 tcaggccggt cgcccggcga gcccggagga tatcaatact gtttattaac tgagagcggg
     9961 gacaggtgct gaatcccggc tggtcggtac gcaccagcgc gtatttttcc acgagaaaat
    10021 taactgcatc acacagtgaa atgcctgcct caatatgctg ctcaatcaca cgttcatcga
    10081 caaacggtgt gtcattcagt gtgaggccat aatgctggtc cagcagtcgg atgagcagtg
    10141 tctgccagat ggtgacggga gacgggcagc gagatacctc ccgtacatgt gtgtcggata
    10201 atgttttcat cttaagttct ctgtaactgg tagtgctaaa ggtgagagct tcggggttat
    10261 gaggtggttg cgggtgctgg tgtcggataa acagccagat aaacgtaacc acaggagccg
    10321 agggtgtcgg cttcgcaggt cagccctttt gcatacagcg tgacggtatg ctgatatcgg
    10381 ggattcagtt caccgctggt gagcatgagt tccagttgtt tcatcagcag cggaaaggcc
    10441 tggtccagat ggtatgcatc tgcgtcgctg aagcggcctc tgataccggc gcggtcggca
    10501 aggtaataca ggcggttacc ctcttgcacc agacgagcgc caaaacaggg cctcactgtg
    10561 cagggaagcc cccaccaggg gcggtcgtga ttgtcatcgg gatgagttat cccggagagt
    10621 ttgtctgaca cgataaaatc ctctctgaaa agtggttaag aatggtttaa cgatgacagg
    10681 tcagccggcg cgctgaccgg tttcggtgaa gatgtatgca ttgatgagaa tggcctcatc
    10741 cacagcggcg tctgaggtca tctggtcgta ctcgtttccc gggtgacgga acatcagttc
    10801 agggtgacac tcatcagccg gacatacggg ccaaaactgt ccttacggcg ttctgcaaac
    10861 acggcaagaa cgccgggaat atcctgcact tcacgaccag tatacgcttc agcactgccg
    10921 tgccagcgat acttaccggt acagaacgga aaaagacggg atgcaggatg ctgttggtga
    10981 atacgcatgg cttcaccacg ggtgatgatt ctcatgatgt gatacctctg aagacagaag
    11041 ataaaagtga aaacaggtgt gatgtggttg tgacgggtta aagcagacca tgttcggcaa
    11101 aggagaaaac ctggttgcca ccgactatca gatggtccgg cacccggata tccaccaggc
    11161 ccagtgcctg taccagacgt tcggtgataa gccggtctgc cttactgggt gtgacttcac
    11221 cggacgggtg attgtgcgcc agcaccacgg cagcggcatt gtggtacagg gcgcgtttaa
    11281 tcacttcccg gggatgaact tccgtacggt tgatggtgcc ggtgaagagg gtttcaccgg
    11341 caatcagctg attctggttg ttcagataca gcacccggaa ctcttcccgc tccagtcccg
    11401 ccatgttcag aatcagccat tcccgtgccg cacgggtgga ggtgaaggcc acgccgggtt
    11461 catgaagatg gcggtccagg gtttgcaggg cccgcagaat gagactgcgc tcgccgggcg
    11521 tcatgtttcc gggcagaaag gaaagctgct gcattgtccc tgcctccatt cagtcgatga
    11581 tgcgcataat ggcgctgcat tccggatgct gcagggcgta atcccgcagc cggtaataat
    11641 ggaccgtcat ggcataacac tccgtacgac aggcatgatg actgtacgtc atcagacagg
    11701 cagcaatgcc ggcggcttcc gggctcattt cagcgcggtt accgttcatg gcattgaaca
    11761 gtacccatgt ttcgtcatca tcgtcatccg gttcgggtgc cataaatgcc ccgccgttgt
    11821 tcagggtgta cagattccag ataccaccgc agtagtcttc acacagacgg tccatccagc
    11881 cgaagacacg gggttccagg gtgacccact gtggaatgag gccaaaatgc tgcggccaga
    11941 agctgatgcg ctgttcatca gggacgggag tggcaaccag ttgttgcagg ccagtctccg
    12001 gtgcacaggc cgaagcggtg gtgttctgag acaatgtttt catgtatgaa atccttgtaa
    12061 tgtgaataaa tgagagggag taaaggtcgg gagctggcaa catcacgttc atcactgacc
    12121 gtgatgaccc ggtaatatca gggatgaatg atattcggct gaaattccgg acagccgtac
    12181 gcgtaaccgt tccggtcaca gacggatggc cagatgccat tgcctgtata ctcagtctgc
    12241 acagactgat ttctccggga cggtaaagca gacgggtaca ccaggcgcac aggtcatcat
    12301 cagaaatgga acgacacccc ggcagcgcct ccggggcgtc gggtgattct ggtgcctgaa
    12361 tcacgccttc agggacatgc tgcctcagcg caggctctcc agcagcgttt ctgccattac
    12421 ccacaatgcg cggttgagct taatatcggt gtcgatgttg tgaatggcac gggtgtggat
    12481 acgttttcct tttgcactgc ggccggaaat cccgcctttc agcatattct cctggatggt
    12541 ctgatatgcg ctccacaggt ctttaccgta atcctccctg cgtcgtggtg tcagaatgtc
    12601 agcggtggtg actggctgat gttcgtcacc ataacggtaa gtcagtgccg cctgtgccag
    12661 cgcctggcgt gccggtggcg gcaggaccag cgactgcatg gcatcacgct tctcctctat
    12721 ccggtcaaac acgcccacca cctcgtaagc cccttcaata actttctcca ccacatttcc
    12781 ccggtgtgga acacgcaatt ctcccagaga ctgaccgcag accagtccat tggtacaaat
    12841 ggcacgaaaa tatccgggta acatctggta gctggatgaa ccgtcatgag agttgagcag
    12901 aataatttca gggacatgct gaccggttat ctgtccggcc cgccgcagac gcagcatgtg
    12961 ttttgtgtat tcccggcgac ccgggtcacg cacacgtgtc tggcaggcga agaatggctg
    13021 aaagccttcc cgctgcaggc tttccagtac ggtgatggtg ggagtgtacg catagcgttt
    13081 actgcgggaa gtatgccggt cttcaccgaa aatactggga acatagtgca tcagttcttc
    13141 gtgtgtcagc ggacggtcac ggcgtatctg gtttgcagca taaccaaaac gactggctag
    13201 tcgcataatt tgctccttat cggtggttaa gatttactgg tgtaataaat gaaaaagcca
    13261 cgtctcccgg agaagacgcg gcctgatgga cgaaattaat gaggtttatt gtctgagaag
    13321 accgtcactg gcgaaccttc cggcgtcagt ctctgcgttg cggataaccc gacggaaatg
    13381 agttccctgg tcatcgtcga taaacacatt gcggtaagct gttgtgatga caacagcctg
    13441 ctcgcgtgag aaagggactt cgggcaataa acgtgacata caaccagacc tgtatttggt
    13501 tccctgaaaa tataagtccc tgtcatcatg gcagagccgt atttactttt gatatcaata
    13561 aattaataac gcgaagatga accgcataac ttacgccttt aatttgtttt gtttacgtag
    13621 tccctgacca ggtacgcgaa ttcgtgaacg ccagtttcac caaaccaaat caggtaacca
    13681 gggtcgatgt tgtaatagta accaacgact ttatgcgttt ttgggttata gagtgttacg
    13741 atatagtccc agccgcaggc gtgggcttta caaccgccac cttctaccca ggtcgttccg
    13801 ttaatgttta caggatacgc accaccatca ggaccatcca tatagccgtc ttccccaaac
    13861 attccgttta acccaacagg gtttaatgct ttgtcgatag cagctctgta ggtagcgtca
    13921 ccttcgtata actcgtacgt tgttggacat gcaccaggct ggtcagcccg ggcttttgag
    13981 tcgcatttaa gtgtctgatt tacagtggcg gcgtttgtgt caggactt
//
LOCUS       DAJTYM010000095        14005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTYM010000095 DAJTYM010000000
VERSION     DAJTYM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..152
                     /locus_tag="OO575_003093"
     CDS             <1..152
                     /locus_tag="OO575_003093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665963.1"
                     /translation="KLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLT
                     IETRD"
     gene            complement(371..1909)
                     /gene="aldB"
                     /locus_tag="OO575_003094"
     CDS             complement(371..1909)
                     /gene="aldB"
                     /locus_tag="OO575_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6665964.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2074..3225)
                     /gene="yiaY"
                     /locus_tag="OO575_003095"
     CDS             complement(2074..3225)
                     /gene="yiaY"
                     /locus_tag="OO575_003095"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6665965.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3415..5259)
                     /gene="selB"
                     /locus_tag="OO575_003096"
     CDS             complement(3415..5259)
                     /gene="selB"
                     /locus_tag="OO575_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6665966.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5256..6647)
                     /gene="selA"
                     /locus_tag="OO575_003097"
     CDS             complement(5256..6647)
                     /gene="selA"
                     /locus_tag="OO575_003097"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6665967.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(6745..7353)
                     /locus_tag="OO575_003098"
     CDS             complement(6745..7353)
                     /locus_tag="OO575_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6665968.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7424..8509)
                     /locus_tag="OO575_003099"
     CDS             complement(7424..8509)
                     /locus_tag="OO575_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6665969.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8512..8874)
                     /locus_tag="OO575_003100"
     CDS             complement(8512..8874)
                     /locus_tag="OO575_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6665970.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9411..11324
                     /gene="mtlA"
                     /locus_tag="OO575_003101"
     CDS             9411..11324
                     /gene="mtlA"
                     /locus_tag="OO575_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6665971.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSTKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11419..12567
                     /gene="mtlD"
                     /locus_tag="OO575_003102"
     CDS             11419..12567
                     /gene="mtlD"
                     /locus_tag="OO575_003102"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6665972.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12567..13154
                     /gene="mtlR"
                     /locus_tag="OO575_003103"
     CDS             12567..13154
                     /gene="mtlR"
                     /locus_tag="OO575_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6665973.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13166..13375)
                     /locus_tag="OO575_003104"
     CDS             complement(13166..13375)
                     /locus_tag="OO575_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665974.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13659..>14005
                     /locus_tag="OO575_003105"
     CDS             13659..>14005
                     /locus_tag="OO575_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6665975.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFS"
BASE COUNT         3390 a         3761 c         3547 g         3307 t
ORIGIN      
        1 ccaaactaat gaataactcc agtaaaacat ccacagtaca gattaagcgt attaaacctt
       61 caattatcta ccgtttattg ctgattggcc tcggatcacc aatggtgatt tacggcctgg
      121 ttcgcccgct caccatcgaa acgcgagatt aaactcctga caaaacacgc cccagaaacg
      181 cacttctgtt ggcgtagtct gggttattgc gcacgtaggt ttaaaacgta aattgtaaca
      241 acgtgcgctt tgtttatgcc ggatgcggcg tgtacgcctt atccggccta cggtcaggtt
      301 cccgtaggca tgataagacg cgtaagcgtc gcatcaggca atgaataccc aatgcgacca
      361 gcttcttata tcagaacagc cccaacggtt tatccgagta gctcaccagc aggcacttgg
      421 tttgctggta atgctccagc atcatcttgt gggtttcgcg accgatacct gattgtttgt
      481 agccaccaaa cgcagcatgt gccgggtaag cgtgataaca gttggtccac acgcgcccag
      541 cctgtatgcc gcgccccatc ttataggcca gattaccgtt gcggctccag acgcccgcgc
      601 ccaggccata ttgcgtatcg ttcgccagct ccagcgcttc ttccatcgtt ttgaaggtgg
      661 tcaccgccag caccgggcca aaaatctcct cctggaacac ccgcatattg ttctgaccaa
      721 acagaatcgt cggttcgagg tagtagccgt ctttcagttc accttccagc agcttgcgcc
      781 gcccgcctgt gagcacgtca gcgccctctt ttttaccgat atcaatgtag ttgaggatgg
      841 tttccagttg cccgtgagaa acctgcgcgc ccatttgcgt cacgctgtcg agcgggttac
      901 cgctgcgaat gctttcgaca cggcggatgg cgcgttccat aaagcgttcg tagattgact
      961 cctgcactaa ggcacgactc ggacaggtgc aaacttcgcc ctggttaaag gcaaacagtg
     1021 caaagccttc cagcgcttta tcgaaaaagg catcttcttc atccatcaca tcagcaaaga
     1081 agatatttgg cgacttaccg cccaactcca gcgtcaccgg aataatgttt tgcgttgcgt
     1141 attgcataat ttgttggccc acttccgttg agccggtaaa cgccactttg gcgatgcgtt
     1201 tcgaggtcgc cagatattcg ccaatttccc cacctgcgcc attgaccacg ttcaccacgc
     1261 ccggcggcag taaatcaccg acaatttcca ttagcagcag tacagaaagc ggggtaagac
     1321 gtgcgggttt cagcaccaca cagttgcccg ccgccagcgc gggagccatt ttccagctcg
     1381 ccatcagcag cgggaagttc cacgggataa tctgccccac cacgcctaac ggttcatgga
     1441 aatgataggc cacggtttcg ctatcaactt cactgatccc accttcctgc gcccgaatac
     1501 acgaggcgaa atagcggaaa tggtcaatcg ccagcggtac atccgcagca ctggtttcgc
     1561 gaatgggttt gccgttatcc caggtttcag ctgtcgctaa cagctcgagg ttttgttcca
     1621 ttcgatcggc aatcttaaac agaatcgccg cgcgatcctg caccgaggtg tgcgcccatt
     1681 tatctttcac tttgtgcgca gcatccagcg ccagatcgat gtctcgtttg cccgaagacg
     1741 ccacttcgca cagcagctgc ccggtcaccg gcgtcagatt ctggtaatac tcaccatcgg
     1801 caggggctac ccattcaccg ccaataaagt tgtcgtagcg ggcttttaac ttgatgggga
     1861 aaccatactc gccgggctta atctgtgctg aagggggatt attggtcatg atcgtctcct
     1921 tgcggtgtga ggtataacaa gggtagacgt gactggcgaa atcttcgcca gtcggtaaca
     1981 gctttacgac agctatcacg aatttacggg caaggattac agagatagtt gaaaaagcgg
     2041 ctgacaattt gccagccgtt gttggaatga tgattacatt gcggcgcgat aaatcaccac
     2101 aatttcttcg tgagttgcct ggatcgggtt agtaaagcca caggcatctt tcagggcatt
     2161 agtcgccaga accgcgaaat cttcttcttt cacgttcagg tcgcgtaggc ctgccgggat
     2221 atccactttc ttcgccagtt cacggatggc gttaatgcag gcttcagcac cttccgcgtc
     2281 gtttttacct gtcacgttca cgcccattgc agcggcacag tcacgcagac gtgcagcggc
     2341 gactttgctg ttgaatacct gaacatgcgg cagcagaacg gcgttacata caccgtgtgg
     2401 caggttgtag aaaccgccca gctggtgcgc catcgcatga acataaccca gagaagcatt
     2461 attgaacgcc ataccggcaa ggaactgggc ataagccatt gcttcacgcg ctttcgcatt
     2521 actgccatct tcaacggcta acggcaggtt ttcggcaatc atggtcacgg ctttcagtgc
     2581 acaagcgtca gtgatcggcg tggcggcaat agaaacatac gcttcgatag cgtgcgttaa
     2641 ggcatccata cccgttgcgg cggtcagtga cttcggcata ccaatcatca gagaggagtc
     2701 attgacagaa agcagcggag tcacgtgctt atcaacaatc gccattttgt tatgacgcgc
     2761 ttcgtcagtg atgatgcaga aacgggtcat ttcagaggcc gtacccgccg tggtattgat
     2821 ggcgatcatc ggcagctgcg gttttgcaga gcggtcaacg ccttcgtaat cgcgaatatc
     2881 gccgccattg gctgccacca gcgcaatacc ttttgcgcag tcgtgtggag aaccaccgcc
     2941 taaggagatc acgctatcgc aattattttc tttaagtaat ttcaaacctg cggcgacgtt
     3001 ttccgtggtg gggttaggtt gggtgccatc ataaataacg ctaaaaatat tgcgttcttc
     3061 cagtgctttt tgcacatcac ccgccatacc taatttcgtt aacatattgt cagtgacaat
     3121 taaggtacgg gtaaatccat aatctgccat catattcatt gcatcagtca atgaatcagc
     3181 gccgatgaca ttcacagaag gaataaagaa agttgaagat gccataacac actcctgaaa
     3241 gtgtaaaaaa gggtgggcag cttactgagg attttcattc gaaaacatga tcattattat
     3301 taattatcaa aattaatcta ataaaaagca cattgtattc attaaacaat gtgctttatt
     3361 taaaatcata agaaatttgt attaatttat tttaaaaatt aattaatcat ttccttattt
     3421 ttccggaaat aataatgcgt cgcgcaataa atgatcattt ccacgacgac gcgtaaagcc
     3481 aatgcggtca aaatattcca gaatctgaat tgccagcttt cggcctacgc ctaagcgatc
     3541 gcggaaatcc gccgcgcagg ttgaaccaca ctcctgatcg agatcgcgga tcatattggc
     3601 aaattcgaca atccgatcgt tacggtaata acgatctttg acgatcgcgg taattattcc
     3661 ttgctgcgcc gcctggcgta gcgtcaggcg cattgcctgc tcgtcggtac ccgtctcttt
     3721 tgccagatca cgcacccacc acggctcgtc accaaacagc ggctctgctt tttgccagat
     3781 ggcctgctgc tcttcgctga agcccgcttt gtgatccggc agatgcagcc aaccgtgatg
     3841 actgaggatg tcgccgcttt cgcgcatctt ttcaatcagc aacagtacta gcgcttcatc
     3901 ttccattggc aacgccatac gtcgcagacg ttcgcgccca ggcccaggtt catcgcgatg
     3961 ttgctcatga taagtcgcta atgtgtcgag aattttccgt tgccagcgag cggcaaccgg
     4021 cgcattcaac aagctataac cagcctgaat atagccaggc tgttgcagca gttcgcgcat
     4081 cccttcgccg ttgagctggc gcgcccaagc gaaatcagga agattaaccg cgccgcgttg
     4141 caggtgaacg gctaacgctt cgctatcatt ctgtgccctg gcgagggaag ccagccattg
     4201 cagatactct ggtttacgtt taccgcgacg cggcgggtta agcatcacga cgcgcgctcc
     4261 ggccagcgtg ttgcgggcag agatatcgcg caataccagg cggtcgttat ctgccagcca
     4321 taacggggtg tcgaagacca gttcagcaag gttatcttcc agcagtgaaa cgcgtcccgt
     4381 gacgtggctg gcggcgtggt gaatatgcag cggctgccac tgggtcagcg gtgcatgggt
     4441 ttgaagctcg acaatcaccc gtgtgaacgg ctctggcggc acatcggcaa gcagccagtc
     4501 gccacggtta atctgctctt tttccgcatc acccgcgatg tcaagcgcga tacgctgccc
     4561 ggcattggcg gtttctgttg gctggttttg cgcatgcagc gcacgtacac gcatcggttt
     4621 atttacacca gtcagccaga gtgaatcgcc taccttcact tccccgctta acgccgtacc
     4681 ggtgacgacc agcccggcac cttttacggt aaatgcgcgg tcaatcgcga ggcggaaact
     4741 atgttggctg gcgtgctcgc gttccggcaa ctgaagcaga tgctcgcgca gggcatcaat
     4801 tccccgacct tcggttgctg cggtgataaa cagttttgcc tcagcaaaac cgtattcccg
     4861 cagaacctcc tttacctggc gttcaacctc atcaacacgc gcttcgtcca cgcgatcggc
     4921 tttggtcagc gccactgtca gcatcgggtt accggtcagc tgcaaaatcg ccagatgctc
     4981 acgggtctgt gccatcacgc cgtcatcgca cgccaccacc aacagcgcgt gatcgatacc
     5041 accaacgccc gccagcatgt tggaaagaaa cttttcatga ccgggaacgt cgataaaacc
     5101 aggcacgcga ccatccggct gcggccagta ggcatagccg agatcgatgg tcatgccgcg
     5161 ctttttttct tccggcagac ggtcagcatt tacgccagta atcgcctgca ataaggttgt
     5221 tttgccgtgg tcaacgtgtc cggcagtcgc aataatcatt tcaacaacat ctccaaaaac
     5281 cgttgctcat cttcaaggca gcgtaaatcc agccacaatc gtccgtcata aatacgacca
     5341 atcaccggca ctggcaattc acgccagcgg gcggctaatg actcaaggtg gctaccgcgt
     5401 ccatcatggg gtgtaaacgt taatgccgcg ctcggcaggc gatcaaccgg cagcgaacca
     5461 ctgccaatct gcgaaagaca tggcataacc tgtaccgcaa actccgcgcc gtaatgtgcg
     5521 acaaggggag cctgtaaacg ttgtgcctgg atttgaatga cctctgcgct gcgggtaagc
     5581 aggcgcaggg tcggtaattt ttcactcaga gcttcagggt gtaaataaag acgcaacgtg
     5641 gcttccagcg ccgcgagggt cattttatcc gcgcgtaatg cacgcttcag cgggtggctt
     5701 tgcaggcggg cgatcatctc ttttttacca acgataatcc cggcctgcgg cccgcctaac
     5761 aacttgtcgc cggagaagct caccagactg acgcccgccg caatcaactc ctgcggcatt
     5821 ggctctttcg gcaaaccgta ctggctaaga tcgaccagcg agccactgcc taaatcagtc
     5881 accactggaa catccagctc tttgccgagc gccaccagtt cagcttcgtc tatcgctttg
     5941 gtgaacccct gaatgctgta gttactggta tgtactttca tcaacagtgc ggtattttca
     6001 ttcaccgcct gacgataatc attcgcgtgc gtgcggttgg tggtccctac ttcgtgtagg
     6061 gtgcagcctg cctgacgcat aacatcggga atacgaaacg cgccgccaat ctccaccagt
     6121 tcgccgcgag ataccaccac ctctttcccg ctggcagtgg ccgccaacat caataacacc
     6181 gccgccgcat tgttattgac gatacaggca tcttccgccc ccgtaatacg gcacagcagc
     6241 tgcgccagcg cccgatcgcg atgtccgcgt ccggcgtcgt ccagatcgta ctcgagggtc
     6301 actggcgaac gcatagcctg cgcaacggct tccaccgcgg cttccgcctg taaagctcgc
     6361 ccaaggttgg tatgcagcac ggttcccgtc aggttgatca ccggacgcag cgcgctctgc
     6421 gcctctttcg tcaaccgggc atcgacttct tgcgcccagt tttcacacca cgcaggcagc
     6481 gtctggctgc cacgaatcac ttctcgcgct tcgtcgagca tctgacgcaa caattccacc
     6541 acgcgggtgt gaccataagt atcacgcaaa gaaaggaagg agctatcgcg caataagcga
     6601 tcaatagccg gaagttgact atagagggaa cgcgtttcgg ttgtcatagg aaacctggct
     6661 gatcaaggcc ctctcacacg gagaagggcg tttaacataa ccacggattg taacgtgaga
     6721 tgggtcagga ggacatatcg cgcatcaagc ctttggcggt tcggtgcgag caaaactttc
     6781 gcggctaaac aggttttcga ccagtttgac taagtgtggg cggtcgacac accaacctgg
     6841 cgcaacgcgg cggaaattga gatatccgac agcacaggca atggcgatag tcgccagatt
     6901 gaccgtatcg gttttgagtg tgccatcgac cagatatcct tccagcacat ccagactgcg
     6961 gttgattttc tcccgctggc gtaacaattc atcttcagac tgctgcgccg ccggacgcgc
     7021 ctgttcacgc accgatacca gcccggcatc cataatgcca tccgccagcg cctcaatttt
     7081 gcgcacccgc aacgactcca gcggatcgcg cggcaacatc gccggagcga cattcattaa
     7141 ctcaatatat tcagcgatga tcggcgaatc aaaccagcat tcgccctctt cggtcaccag
     7201 caccggcact ttccctaacg ggttaaattg cgccacgccg ttatccgcgt tatagggcag
     7261 ttcattaata aattcgaaag ttatgccctt ttctaacaac agaataaaaa gtttgcgtac
     7321 aaacgggctg gtgtagctac cgacgagttt catgccgagt cctttgtgcg aggaaaaata
     7381 tcagtatggc tcaggctcac gcaaaacaca ggtgatataa gtctcaatga tccaaataaa
     7441 gataatgcat ccaactggtc attcttagca gcactttccg catcaccgaa acatggctga
     7501 aatggtcgga gtaaaccgcc acctgggcgt agatgccgtc gggtaaggca tcgatatcat
     7561 cgttagggtc cagttcaatg gttcccagca caccgtccgt gccgggaacg accgttaatg
     7621 attgcaatac cccctgcgcc tgataagaac cgcctggcac gacaggtaaa atactagtca
     7681 atttgccgtg gaacacctgc ccaggtagcg cgttaaacac cacttccgca tcatcgccag
     7741 gtttcagacg taacagcgag ttttgccgaa attgggcgac aatttgccgt ttttgctcgg
     7801 ggatgaagac catcaccgga cgcagcggca aggcagctgc gtatgtacct gggcggatca
     7861 gtacctgagt gacgtagcca ttgcttggtg cgcgaatgac agtctgctca agattatatt
     7921 ttgcttcagt aagttgcgct cttaagctca caatctgcga ttgctcgccg ttgaccatac
     7981 tgtcgagctg actctggatc tgcgcctgct ccgccaccga gcctttcact aacgcatcct
     8041 gcgcgaggaa attttgccgc gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg
     8101 cctggctgcc tttcaggtaa cgttgataat ttttaaacag acggtcgcgc tccgctgaaa
     8161 cctgggtggt gttggcctgc gcttcagtga gctgcgcacg cagcgtcttt atattatgcg
     8221 tcgccgtcat caggtcagcc tgaagtctgt caactcgcgc ctggtaacga accgggtcga
     8281 gcttaaaaag cacctcgccc ttttgaataa gctgattatt cttgtcagtg acttcagtaa
     8341 caattcccgt cacctgtggc gtgataggga tcgctatcac tgccttttgc gcggtaaaag
     8401 tgtaagggtg gttgtagttc atcaacaaaa tcaaaccact caccagaaac acgcctccca
     8461 gcgccgccgt cgccagcgcc acgtaagtta aaacaatcaa tagatccata attagatctc
     8521 cgggaacgtg gaatagtcag tctttttctc ggcggcgagt tgatgctcca gccgggaaat
     8581 tcggtcagaa agtgcggcga tttccggttc agtcgctttc tcctgcgacg caacatgtga
     8641 ctgcataccc cagccacgct ccggttgata gagcgtcgcc cagatccaca gaaacggcca
     8701 gataacatgc agagtaaaca ggctcaccca gcccgccgta tgaatagcgt cggcatgggg
     8761 gtgattgcgc tttttggcaa tcagataagg gatgtcatgt atcgcgatga ttccataaaa
     8821 aatcaccaga aagacgaaga tcagcactcc caacgcgaaa tagtttagaa acatatctgc
     8881 ctcggattca cgtttatcag tgttgttttt tggctggcag ccagaaggga gtcaggctga
     8941 tattttgaca ataatccggg ttcgcgattc tcgccataac accaaagaat aatttttaga
     9001 ggtgatgagt tgcttagtta cataacgatt gtatgacgaa ggcataacat gctgtagatc
     9061 acatcaggtg aacgccgtaa gaaaatatct tgtgattcag atcacaaaga ttcaacaaac
     9121 catcaaaaca aaaatgtgac attactcaca tttaaatgcc atttttagcg aaaatcaccg
     9181 ccttgtttct tttttataca ggcgttttgt gatgagcgtc acgtcaatta cctctctacc
     9241 ccctatattt atgtgactga tatcacacaa aagcccgtcg actggacagt taaccgattc
     9301 agtgccagat ttcgcagtat ctacaaggtc cggctacctc tgccgccaca ttaacaaaaa
     9361 acctcgggct tccagcctgc gcgacagcaa acataagaag gggtgttttt atgtcatccg
     9421 atattaagat caaagtgcaa agctttggtc gtttcctcag caacatggtg atgccaaata
     9481 tcggcgcgtt tatcgcgtgg ggtatcatca ccgcgttatt tattccaaca gggtggttac
     9541 cgaacgagac gctggcgaag ctggtcgggc cgatgatcac ttatctccta ccgctgctga
     9601 tcggttatac cggtggtaag ctggtaggcg gcaaacgtgg tggcgtagtc ggtgccatta
     9661 ccaccatggg cgttatcgtc ggcgcagaca tgccgatgtt cctcggttct atgattgcag
     9721 gtccgctggg cggctggtgc attaagcact tcgaccgctg ggtagacggt aagatcaaat
     9781 ccggttttga gatgctggtg aataacttct ccgcaggcat catcgggatg atcctcgcta
     9841 ttctggcatt cctcggcatt ggcccgattg ttgaagccct gtccaaaatg ctggctgcgg
     9901 gcgttaactt catggttgtc catgacatgc tgccgctggc gtctatcttt gttgaaccgg
     9961 cgaaaatcct gttcctcaac aacgccatta accacggtat cttctcgccg ctgggtattc
    10021 agcagtccca tgaactgggt aaatcaatct tcttcctgat tgaagctaac ccaggtccag
    10081 gtatgggcgt gctgctggcg tacatgttct ttggtcgtgg tagcactaaa cagtctgcgg
    10141 gcggtgcggc aatcatccac ttcctgggtg gtatccacga aatctacttc ccgtatgtgc
    10201 tgatgaatcc gcgtctgatc ctcgcagtca tcctcggcgg tatgactggc gtgttcacgc
    10261 tgactatcct gggcggtggt ctggtttctc cggcatctcc gggttctatc cttgctgtac
    10321 tggcgatgac accaaaaggt gcttatttcg ctaacatcgc gggtgtgtgt gcggcgatgg
    10381 ctgtctcctt cgttgtctct gctattttgc tgaaaaccag caaagtgaaa gaagaagatg
    10441 atattgaagc agcaactcgt cgtatgcagg acatgaaagc tgagtctaaa ggcacatctc
    10501 cgctatctgc gggcgatgtg actaacgacc tgagccacgt acgtaaaatc atcgttgcct
    10561 gtgacgccgg tatgggttca agtgcgatgg gcgcaggcgt gctgcgtaag aaaattcagg
    10621 atgcaggtct gtcgcagatt tcggtcacta acagcgcgat caacaacctg ccgccagatg
    10681 tggacctcgt catcactcac cgtgacctga ccgaacgcgc tatgcgccag gttccgcagg
    10741 cacagcatat ttcgctgacc aacttcctcg acagcggcct gtacaccagc ctaaccgaac
    10801 gtctcgttgc tgcccagcgc catactgaaa acgaagtgaa agtgaaagac agtctgaaag
    10861 acagctttga cgattccagt gctaacctgt tcaaactggg cgcggagaac atcttcctcg
    10921 gtcgcaaagc agcaaccaaa gaagaagcga ttcgttttgc tggcgagcag ctggtgaaag
    10981 gcggttacgt tgagccggaa tacgttcagg cgatgctgga tcgtgaaaaa ctgaccccga
    11041 cttatctggg tgagtctatc gcggtgccac acggtacggt tgaagcgaaa gatcgcgtac
    11101 tgaaaacggg cgtggtgttc tgccagtacc cggaaggcgt gcgcttcggt gaagaagaag
    11161 atgacattgc ccgtctggtg attggtattg cagcccgtaa caacgagcac attcaggtta
    11221 tcaccagcct gaccaatgca ctggatgatg agtccgtcat cgagcgtctg gcacacacca
    11281 ccagcgtgga tgaagtgctg gaactgctgg caggtcgtaa gtaatccaat cctaccctct
    11341 cctcacggag agggtttggg tgagggaaaa gcctcacccc agccctctcg ggtaaaaaca
    11401 ttgatgaagg ttaatactat gaaagcatta cattttggcg caggtaatat cggtcgtggc
    11461 tttatcggta aactgctggc agacgcgggt atccaactga cgtttgccga tgtcaatcag
    11521 gtggtacttg atgccctgaa tgcccgtcat agctatcagg ttcatgtggt cggtgaaacc
    11581 gagcaggtag ataccgtttc cggcgtcaat gctgtcagca gcattggtga tgatgtcgtt
    11641 gatctgattg ctcaggtcga tttagtcact accgccgttg gcccggttgt gctggaacgt
    11701 attgctccgg ctatcgccaa agggctggtg aaacgcaaag aacaaggtaa tgaatccccg
    11761 ctgaacatca tcgcctgtga aaacatggta cgcggcacca cgcagctgaa aggtcatgtg
    11821 atgaacgccc tgccggaaga cgccaaagcg tgggtagaag agcacgttgg ctttgtcgat
    11881 tccgccgttg accgcatcgt accgccttcg gcttcggcaa ctaacgatcc gctggaagtg
    11941 acggtagaaa ctttcagcga atggattgtc gataaaacgc agttcaaagg cgcactgccg
    12001 aacatcccag gcatggagtt aaccgacaac ctgatggcat ttgtcgaacg taaactcttc
    12061 accctgaaca cgggtcatgc tataaccgcg tacctcggaa aactggccgg tcatcagacc
    12121 attcgtgacg ctattctcga cgagaaaatc cgcgcggtgg taaaaggtgc gatggaagaa
    12181 agtggtgcgg tactgatcaa gcgctacggc tttgacgcag acaaacatgc ggcgtacatc
    12241 cagaaaatcc tcggtcgttt tgagaacccg tatctgaaag atgatgtaga gcgcgtaggc
    12301 cgtcagccgc tgcgtaaact gagtgctggc gaccgtctga tcaagccact gctcggtacg
    12361 ctggaataca gccttccgca caagaatctg attcagggga ttgctggtgc aatgcacttc
    12421 cgcagtgaag atgatccgca ggctcaggaa ctggcagcac tgatcgctga caaaggtccg
    12481 caggcggcgc tggcacagat ttccggtctt gatgccaaca gcgaggttgt atccgaggcg
    12541 gtaaccgctt ataaagcaat gcaataatgg tggaccaggc gcaggacacc ctgcgcccga
    12601 ataacagatt gtcagatatg caggcaacaa tggaacaaac ccaggccgtt gaaaaccgtg
    12661 tgcttgagcg tctgaatgct ggcaaaaccg tgcgaagctt tctgatcacc gccgtcgagc
    12721 ttttgaccga ggcggtaaat cttctggtgc ttcaggtatt ccgcaaagac gattacgcgg
    12781 tgaagtatgc tgtagaaccg ttactcgacg gcgacggtcc gctgggcgat ctttctgtgc
    12841 gtttaaaact catttacggg ttgggcgtca ttaaccgcca ggaatacgaa gatgcggaac
    12901 tgctgatggc attgcgtgaa gagctaaatc acgacggcaa cgagtacgcc tttaccgacg
    12961 acgaaatcct tggacccttt ggtgaactgc actgcgtggc ggcgttacca ccgccgccac
    13021 agtttgaacc agcagactcc agtttgtatg caatgcaaat tcagcgctat caacaggctg
    13081 tgcgatcaac aatggtcctt tcactgactg agctgatttc caaaatcagc ttaaaaaaag
    13141 cctttcaaaa gtaagcaacg tctgcttact gcccctctac ctgcttcggc cgataaagcc
    13201 gacgataata ctccagacgt tgtagatata acggcacgct ttcatcgggt attccggacg
    13261 gaatcgcgtt acggggagga agttttttca gatactcccg gaacgcctgg cttgatgcca
    13321 tgaaatctac ggctttatcg ataagaagcg gaacgttttc acctaatttg cccatgatga
    13381 tatctccgta ttaccccgcg ccgggaatgc gcggccgcca attttagttt aggttcccgg
    13441 taaagatgac attaaggaaa acgtgctgaa tcctcaataa tcatccgctg ctgtttatta
    13501 tctttttcta tagatttctt aattaatcaa cgaattgcgt agtttttgag catgttaatg
    13561 attacgttgc agaactatta caatgtcatc agttgtgaac aaagcacctg gtcgcgcata
    13621 ctaggggcta taaatttatc tttatcagaa gccatcacat gaaagaagtc gaaaaaaacg
    13681 aaatcaagcg tctcagcgat cgcctggacg ccatccgcca ccagcaggcc gatctgtcgc
    13741 tggttgaagc cgcagacaaa tatgccgagc tggaaaaaga gaaagccacg ctggaagcag
    13801 aaattgctcg cctgcgtgag gttcatagcc aaaaactgag taaagaagca cagaagctga
    13861 tgaagatgcc gttccagcgc gcaattacca aaaaagagca ggctgatatg ggcaagctga
    13921 agaaaagtgt tcgcggactg gtggttgtgc acccaatgac cgcgctgggg cgcgaaatgg
    13981 gcctggaaga gatgactgga ttttc
//
LOCUS       DAJTYM010000096        13477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTYM010000096 DAJTYM010000000
VERSION     DAJTYM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1230)
                     /locus_tag="OO575_003106"
     CDS             complement(<1..1230)
                     /locus_tag="OO575_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6665976.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQG"
     gene            complement(1273..2647)
                     /locus_tag="OO575_003107"
                     /pseudo
     CDS             complement(1273..2647)
                     /locus_tag="OO575_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2693..4729)
                     /gene="yihQ"
                     /locus_tag="OO575_003108"
     CDS             complement(2693..4729)
                     /gene="yihQ"
                     /locus_tag="OO575_003108"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR6665977.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            complement(4928..5830)
                     /locus_tag="OO575_003109"
     CDS             complement(4928..5830)
                     /locus_tag="OO575_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6665978.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(5968..7209)
                     /gene="yihS"
                     /locus_tag="OO575_003110"
     CDS             complement(5968..7209)
                     /gene="yihS"
                     /locus_tag="OO575_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6665979.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(7226..8104)
                     /gene="yihT"
                     /locus_tag="OO575_003111"
     CDS             complement(7226..8104)
                     /gene="yihT"
                     /locus_tag="OO575_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6665980.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(8128..9024)
                     /gene="yihU"
                     /locus_tag="OO575_003112"
     CDS             complement(8128..9024)
                     /gene="yihU"
                     /locus_tag="OO575_003112"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6665981.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            9192..10088
                     /gene="yihV"
                     /locus_tag="OO575_003113"
     CDS             9192..10088
                     /gene="yihV"
                     /locus_tag="OO575_003113"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6665982.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            10122..10907
                     /locus_tag="OO575_003114"
     CDS             10122..10907
                     /locus_tag="OO575_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6665983.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            11006..11605
                     /gene="yihX"
                     /locus_tag="OO575_003115"
     CDS             11006..11605
                     /gene="yihX"
                     /locus_tag="OO575_003115"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6665984.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            11599..12471
                     /locus_tag="OO575_003116"
     CDS             11599..12471
                     /locus_tag="OO575_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6665985.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            12468..12905
                     /gene="dtd"
                     /locus_tag="OO575_003117"
     CDS             12468..12905
                     /gene="dtd"
                     /locus_tag="OO575_003117"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6665986.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            12950..>13477
                     /gene="fabY"
                     /locus_tag="OO575_003118"
                     /gene_synonym="yiiD"
     CDS             12950..>13477
                     /gene="fabY"
                     /locus_tag="OO575_003118"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6665987.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMG"
BASE COUNT         3297 a         3414 c         3531 g         3235 t
ORIGIN      
        1 accctgtaat gtcgcatcgc tttgcgcgat gttgggaaca taaccaattt gcgtcagcat
       61 aatgcctggc aagaagccag caagtgcggc agaaatttta cgaaagaagg tataaccggt
      121 ataaacagaa ccttcggcac gaataccggt tttccattca ccgtaatcta cggtatctgg
      181 taccagtgcc cagttcaggc tgtttacaaa ggccgtgcca aaaaaggcga cacaagagaa
      241 catgacgaaa gtgaaagagt tacttcccca gaaataattc agtatatcac ccaccgccca
      301 tagcaccatg ccagctaaat aaacctgttt tttaccaaaa cattttacag ttactggcac
      361 cagtaatacg ccgacgagga tgcatcccat actgaagaat cccatccacg acaataaatt
      421 aatatcattc agcacatact gggtgtaata gacctgaatc gccagtttga tattaaatgc
      481 cgccagagta cacaggttag caatgcataa aaccagcaat ggtggattac gaaatatcgc
      541 gcaaaaagat ttgagaatac tggctttatg tccggttggc gctgtgtcca cataatgctc
      601 tttgacgcct ctgtagcaca gcatcataaa aataaagccg ccaatggaga acataagtgc
      661 cgcacaggca taaccgacgg ttgagtcaga aaaaagcgac tgcaacggaa taaacgccac
      721 ggtacaaatc aatagcccta tggttgcacc accctggcgg taagccgcga gctgcgcgcg
      781 ctcattcggg tttttggtaa ttgccgggat catcgcacca tacgagcagt tcatcagact
      841 gtatgaaagt ccaaacatca taaacagcgt ggtggcaatc gtcgttttaa ccggcaaaca
      901 aaaggtggtg gcgataaact ggagcgttgc aattaacgcc gccggaactg ctgcatataa
      961 aataaaggga cgaaatttgc ctttaggccc aatatttttc cgcgagtcga gtaaaaatcc
     1021 ggtgagcata tcggtaaacg cggtaaaaaa cttcgcgacc agaaagataa tcccgccata
     1081 gtaagcaggc atccctaatt catcggtata aagtttgagg agatacagag taccgatgca
     1141 gagcattaaa ttcgaaccga cgtcgcccat accataggcg attttttccc gcagattcag
     1201 ttttaatgtc agtggattat ggtcagacat aatcactcct tataacgccc cgcagggcgt
     1261 cgtttattat tattacgccg tgcgtttacg ggcttctatc tcttccacaa tgcggacata
     1321 catcttctcg ttcaggctgt agaagcaacc catcgccaca atggtgacta ccgccagtgc
     1381 gcttgggtag atgaagatca actggcgtaa cccttcaatg gtgtggtcag cctgtgcgac
     1441 gttcggcaca tagccaattt gcgtcagcat ccagccgggg aagaaaccag ccagcgcctg
     1501 agacactttg cgaaagaagg tgaagccggt gtagaccgtg ccttccgaac gcacgccggt
     1561 gcgccactcg ccgtactcga cggtgtcgga aaccagcgcc cagttcaggc gctgagccga
     1621 agaacgccag gcaggagaac gccacaaagc tgaccgagcc gccgccgaag aaatagttga
     1681 gcagatcgcc cagcacccaa atcagcaggc cgccgatata gaccttcttc ttaccaaaac
     1741 gtctgactgc gccaggcatc aggaacacgc cgatgaaaat acagcccatg ctgaaaaatc
     1801 ccatatacga caacaggatg ggatcgttaa gtacgtactg ggtgtaatag acctgaatgg
     1861 cgagcttgac gttaaacgcc cctaaggtgc agaggttggc aatacacaga atgaacagtg
     1921 ggcggttacc ggcaattgcg cggaaagatt gcaacaggcc aggcttttgc gccggattgg
     1981 ttggttgggt ttcgacataa cgctctttca cgcctgagta gcagatccac ataaacagca
     2041 ggccgaacag tgaaaacagc gtggcggcga agatatagcc aagtttctga ttaccttcga
     2101 taagattcat aactggcacg aatcccaccg tgcacagcag caggcccagc gtagcgccgc
     2161 cctgacgcca tgccgccagt gaggcgcgtt cgttggggtt tttggtgatt gccgggacca
     2221 ttgcgccgta ggagcagttc atcatgctga aaaacagtcc gtaaagcata aacagaatag
     2281 tggccatcac cgttttaccg gtgacatcaa acggtgtgcc gacaaagttg gcgatcgcca
     2341 gtaaggtgac cgggaatgac gcatacagaa taaaaggacg gaacttacct tttggaccga
     2401 tcttgcgtcg ggaatccaac ataatgccgg tacccatatc ggtaaacgca gtaaagaatt
     2461 ttgaaatcaa aaagataatg ccgccatagg tgcctggcag ccccagaacg tcggtataaa
     2521 acttcaaaag ataaagcgta ccgatatcca gcaggatgtt agaggccagg tcgccaatac
     2581 cgtaagagag tttctcttta aagggcaggc gtaaagttgc tggatcttcc gttgtgatgt
     2641 gactcatcga tgttctccct ttgcccatga gtttccccac gggcggatgt gattagatgc
     2701 tgtttaacga cgcgaacagt gccgcccatt cgctatcggc gcgataaaag accggcggct
     2761 tgccgatggg cgcattaacg gtaacttccc cgccccggaa cgcttcaccc gtccaggcat
     2821 gtacccagtt atcctccggc agatagagcg tccagtcgct gcgaccttct tcgtgtaccg
     2881 gagcgaccag aatgtcgcgg cctaacaggt actgatattt cagggaatag gtatgtgcat
     2941 cgtcttcgta atgcaggaac agcgggcgca tgaccggcag accggacttc gcattcagcg
     3001 cgacggcctc tttcagataa ggtttcaggg tggtgaaaac ggtagtcata cgggcaaaat
     3061 gggcgatagt ttccgcatcg ccgtcaaact gccagttgtc gccaggacgg ttaccttcgt
     3121 gggtgcgcat catcggcgtg aaggcgctga aatcgcacca gcgcagcagc agctctttgc
     3181 tgcgcttcat ctcaaacagg gtggtgtaac cgccaatgtc gctgtgatgc agaccatgtc
     3241 cggtcattgc cagcgacagc gccgccggga ctacggacgc caggccatcg tcgagactcc
     3301 agtccacgtt ctggtcgccc gcccacatca tggtggagta tttctggcta ccggtagaac
     3361 cggcgcgcat aaagaagagg atctcgccga gcttgcccgt ttcttcaagg gcttcgtagt
     3421 tacacttcgc ccacagcgca ggccaggcgt tatgcataat ttcggcactg acgccgttat
     3481 gcaggtacgt gtcggtgggc agatactcgc cgaagtcagc catccagccg ccgcagccga
     3541 gttcaatcat gttctttttg atcacttcct tgaaccaggc gtaggcttct ggattagtga
     3601 gatcgacaac gccgccgtaa aactcgccaa actccaccag atagtcaccg ccagaggcat
     3661 cttttgctag atagccgtgt tgtgccgctt cttcgcagag atctttatcg ctggcaacat
     3721 acgggttgat ataggccagg aactgcacgc cctcctgata ccactgctta atgcgtgaat
     3781 ccagttgcgg gtagttttcg ctgttccact tccagttcca catcacgcgc ttgccaaaag
     3841 aggttatacg gatcccagac cagtcctgcg cccagatgcc gttaaccttc acgcccgcgt
     3901 tacgcatggt gtccagtttc ttctggcaca cttccgtccc gccctgaatg ccgagcgtta
     3961 ccccgtcata aatccagtcg ggcagttctg gctggcgtcc cagcagggca gttaattttt
     4021 ccagcaggga gatgtatgtg tcagcacatt caaaacgtag cgtagctttg tcttcccaca
     4081 gcgccagttc atggtattcc ggggcgctaa agtcgaagtt catgtagcaa ctgttatcaa
     4141 catggcagta atacttctgc gtgctgacaa acgtaggctg tgggaagaaa gtccagtaat
     4201 agtcgccgcc cgcattttct ttgcagtcgg cctgccaggt gacataggtt tgtttgttgc
     4261 gaccaacgcc ttgttcactg gtccatagcg ggaacggttt gccgcgcaga tcgaagtagg
     4321 aaaactgttc gccgcagccg tagatatggt cctctggttg agcggcaagg cgcagccaga
     4381 tacggttgtg gttaaggttg tcgttttgta gttccagcaa taaacggccc tgatcgtcgg
     4441 cagagatatt cagcgtggcg ctaatgtcag aaccacggct gaaatgaatt aaccaaccat
     4501 ccggtgactg gctgacgatg gcgtcggtaa gcgcaatttt ctcctgtagt ttatctttaa
     4561 tgctgaaatt accgcggaac atatcgatat ccgctatacc tgagccaatc cataaacaag
     4621 gattatcttt gttatgggtt aaaataagac gttgttgaaa atgtagggta aaactgtcgt
     4681 tattctgatg aaattgaaaa tctaataact gtggacgtgg cgtatccata actaatctcc
     4741 atagagttga gaacgagatt gttgccttcc tgttattaca ggcttatgta aaatgattct
     4801 cataaaactg atatataaaa actttacaga agctgagctg tttttaactt aataaattga
     4861 agatacataa tgttgccttt agcgagtgaa atgttcttca atgattttga ctgctaatca
     4921 atacaagtta ttttctctgt ccgtaaatat taaaaaacag gcggtgcggt tttcctggtt
     4981 ccagtaataa taagtcgata cctgaattaa aggcgttagg cgggcaactc ataggttcaa
     5041 ctgccagtcc ctgacgctgt aatttttcac cgctataaac ctgcacccag ggttgatcgg
     5101 aacaaaggct taccgaaagc gcctgctgtg gatgggtaat ggtcatttcc cacaaatcat
     5161 tagcggtttt aaaggtgtgg tcgatttttg tcgcggctat ttttttcgcc tgcgtgaaat
     5221 ttaaatccag ctcatcaacg tggtgaagcg ttgtcggatt tacgtgttca tcgacggcat
     5281 aaacctggtt tgccggaagc tggaacagat attcatcaac agatgttaag ttgcaggtta
     5341 aataaggatg aatacccacg ccataaggcg ctgcgacagt gccgatattc tggcttgcga
     5401 tctcaacgga aagacccgtg tgtgcgttga gcgaataaac aacttgtgaa gccagcataa
     5461 acggatagcc gtaactgggc ggcagaaaag ctgtcagggt aacgctggtg gtggttaatt
     5521 cactaatttg ccagtctcgc caggcaagca gcccgtgaat agcggctttt gaactatgtt
     5581 cattaatggg taactgatat tcctgcccct gataacggta acagccgttg gcgatccggt
     5641 tcggccacgg aataagcact ttacccagat gcgccagcgg catctcttcg ggtttgtgcg
     5701 ggatgactaa atgacaaccc tgaaaggtta attcagccag accagcaccg acggtcacaa
     5761 tggtcgcgtg ataatccccc gcggcaagtg aaacggtctg tccactgcaa tgcatattgg
     5821 tgatttgcat acaaggcacc ttaattaacg acatacagcc tcccgtgagg ctgatgtcaa
     5881 acccaaaaac tgcaccggac ggtcccctcg cccctttggg gagagggtta gggtgagggg
     5941 aaaacccgca ccctattttc agatcagtta tttcgcatta atatccagca gacccgctgc
     6001 aaccgctgga gccatgccgg gcgctaacgg gatacgcggg atcaccaggc aatgcagcag
     6061 gtgataaata tcctgtttgc cgtcccagac tttggtgatg accttattgt ccgcatccag
     6121 ctcctgccac caggaaccat tttcatagtc catcaggtac ttaatgcagt actcccacca
     6181 tgtttgatac caggtttcat actggcgatc gccagtgacg gtgtagagcg cgtagaccgt
     6241 acccattgct tcgacgatag gccaacgtac acgttcgcgg accaccggtt ttccttccca
     6301 gtcaacggta taaacaatcc cgtccgctcc atcgggtgcc caggcatcgc gcacggtggc
     6361 gttaaacaga cctttggcat cttctagcag ccatgctggt ggttgttcgc aacgggcttc
     6421 cagggcagca tggatgtgca gcattaaacg gccccattcg atccagtggc ctggtgtacc
     6481 tccgaacgcg cggaagcgat gcgccgggtt atctttgttg taatccggca gcggattcca
     6541 ctgggtatcg aagtgttcgt taacgcgata atgattattt ctggcgacgt cgtggataat
     6601 cacggaagcc acgcgaatcg cgcgatccag ccattttttg tcgtgagtga cgtcataaac
     6661 aatcaagaaa gcttccaccg cgtgcatatt ggcattgccg ccgcggtact cttcggtttt
     6721 gctgaaggct tcgtcccagg actccaggca catctgctct tcttcgctcc agaaatattt
     6781 ctcgataatc tcaatggtgt aatcgagcag cttgcgcgct tccgggtgac ccgttgtgac
     6841 ggcgctggcg gcacccagca gagcaaagaa gtgttgatag ccctgtttgg aggcatccac
     6901 cacgccctcg tcattcacgc aggcatacca gccgccatat tttttatcac gcagtgcgcc
     6961 gttcatggct ttaataccgt gatcaaccaa cgcgtaagcg ccaggtcgac ccatcgccgc
     7021 agcaacggaa taaacgtgca acatacgagc tgtgatccac agatgggtgc ccatctcttc
     7081 tttgatttgc cctttattgc ctaaccagcc aaaaccagtc ggcactacgg aatttttacc
     7141 aaaatcaaag atgcggtcgg tttcctgttc cagccaacgg ttgtggctta gggtgttaaa
     7201 ccatttcatt ccttttatcc tcatcttagc ggcgtttagc catcatttcg tcgacgatat
     7261 cgccaagttg ttgtaatttc ggtgcgcaaa cgtcacggag catcagctcg ttgtctggta
     7321 aaccgacgac cgatgcccag actgcacggc ctgccaggaa tcccgatgcg cccgccgtca
     7381 ttgccacgcg tacggcacgc gggaacagtt tttcgtcgac actggaagag aggatcaccc
     7441 atggcatatt gatatggtca ttcagacgtt gtgaagcaca gagaagctct tgttgcggac
     7501 ctttgccata aagcggcatt tcaactttgt agaggtcagc gccactgtcg cccagctctt
     7561 tggcggcatc gatgatcgct tgttcgcgat cgaatttatc gccacgacgc ggtgggcgga
     7621 cgactggctc aatgatgctt accagaccgt gtgagtggca cagttcgttg aactctttca
     7681 ccatatccag acgttgctgc gcatcttcat cactacgcca cagcaccagc agttttaagg
     7741 ctttgccacc gtcttgtttg atctgtagcg gattgatttt gcggtcaata accacgctat
     7801 cgaccggaat accgttgcca ggaatgaact catcggcggc gacaatcatg gcgcaacttt
     7861 tggcaatcgc gttttgctca accacctggc ggtagcagaa ttgttgatct accagaatcg
     7921 ccgaggcata aggcgagagg gcctttgcag cgttaacttt gaaatcagtt aaaacgctat
     7981 cggctaccgg agcgggtgcc ccagccgcgg caaacatcat gcgcatggct tcgcgctgat
     8041 cgaccgccag catggcaaaa ccgcccgatg cgcgcgtaat gtcgttgatg gtgtacttat
     8101 tcattcattt attcctttat ccagtcgtta catttttact ttggcagtca tcccggcact
     8161 gacacggacc tgttccagaa tggcggacca gtcctggcga ccgcgacccg ctgcgcgcgc
     8221 ctggctataa acctcccgtg aggcggcccc cagcggcatt ggcacatgca gctggttggc
     8281 gacatcaagg gcgatgccaa gatccttatg ggcaagatcg atcatgaagg cgggagaaag
     8341 atctccgctg aggactttgt ttggccagga agtggtgaag tgacctttac cggcggcggt
     8401 accgctcatc actttgacgg caacatcgaa gggaagattc agggcttcgc acaaaacggc
     8461 ggcttctgcc gaaagcgcat tgagcgcgat gctcatgtag ttgttgatga gcttaacgcg
     8521 gatccccatg cccggaccgc cagcgttgat caactcactg cccatcgcca tcaggatcgg
     8581 tgtggcacgt tcaacttgtt cagcggtgcc gccagccagc agtaacagag taccggtaat
     8641 ggcatttgca gaagtacggc ctaccggaac atccatcatg ctgaagcctt tggcttgcat
     8701 atcggcaatc aatttatcgg tttgcagcgg atggatggtg gacatatcaa tgaccagcgc
     8761 atcggtagat aagccttcgc aaacgccgtt ttcaccgaac aacacgttgc gcaccagatc
     8821 gccattcggc aacatggtaa tgataaattc ggcatcttta gcggcctgcg ccgggttggc
     8881 ggcgggagtc gcacctttgt ctaccagatg ccgcacagct tcggcgttca catcaaagac
     8941 gcgaagttga tgcccttgct gcaataaatt gctcgccatt ggcgaaccca tttgtcctaa
     9001 accgataaac gcgattgctg ccataaccct ctcctgaata cagtgatgtc actttttgtc
     9061 atttatgaca tgctttgctt gtctgttttt gatcgaattt gtaatttatc gtcaaaaaat
     9121 tgacagccgt cactttttaa acaattggtg aaattaaaat gaacgcatcc caaaatgttt
     9181 aaggaatgac catgattcgt gttgcttgtg taggtataac cgtgatggat cgcatctatt
     9241 acgtggaagg gttaccgacg gagagcggta aatacgtggc gagaaattat acggaagttg
     9301 gtggcgggcc agcggcgacg gcagcggttg cggcggcaag gctgggggcg caggtcgatt
     9361 ttattggtcg cgtaggtgat gacgacgcgg gcaacagcct gctggcagag ctggaatcct
     9421 ggggcgttaa cacccgttac accaaacggt ataaccaggc gaaatcttcg caatccgcca
     9481 tcatggtgga taccaaaggc gagcggataa tcattaacta ccccagcccg gatctgctgc
     9541 ctgacgcaga gtggttggag gaaattgatt tctctcagtg ggatgttgtg ctggcagatg
     9601 tacgctggca cgacggcgct aaaaaagcct tcaccctggc ccgtcaggcg ggtgtgatga
     9661 ccgttctgga cggggacatt acgccgcagg atatcagtga gctggtggca ttaagcgatc
     9721 acgcggcctt ttcagaaccg ggtctggcgc gcttaacggg cgtgaaagag atggccagtg
     9781 cgctaaaaca ggcacaaacg ctcacaaatg gtcatgtcta tgtgacccag ggtagcgcag
     9841 gctgcgactg gctggaaaat ggtgggcgtc agcatcaacc ggccttcaaa gttgatgtgg
     9901 tagataccac aggtgcggga gatgtttttc acggcgcttt ggcggtggcg ctggcaacaa
     9961 gtggggattt agcggagtca gtccgcttcg ccagcggtgt agcggcgtta aaatgcacac
    10021 gtcccggtgg acgagccggg atccctgact gtgatcaaac ccgatctttt ttgtcacttt
    10081 ttgtataaaa tgccagggtg atggtttttc gaggaatttt catgagcctt accgaactaa
    10141 ccggtaaccc gcggcacgac caactcctca tgctgatcgc cgagcgtggg tatatgaata
    10201 ttgatgagct ggcaaatctg ctggatgtct ccacgcagac ggtccgccgg gatattcgta
    10261 aattaagcga gcaaggcctg attacgcgcc atcacggtgg cgcgggtcgg gcttccagcg
    10321 tcgttaatac ggcgttcgag cagcgtgagg tttcgcaaac cgagaaaaaa aaagcgattg
    10381 ccgaagcggt ggcagactat attcctgatg gttcaacaat atttatcacc attggtacga
    10441 ctgttgagca tgttgcccgg gcgttactta accataatca tttgcggata atcaccaaca
    10501 gcctgcgtgt ggcgcatatt ctttaccaca acccgcgctt tgaagtgatg gtgcccggcg
    10561 gtacgttgcg ctctcataat agcgggatca ttggcccttc agcggcgtcc tttgtggctg
    10621 attttcgtgc tgattatctg gtaacaagcg ttggggcgat tgagagcgat ggcgcgttga
    10681 tggagtttga tgtaaacgaa gctaacgtgg tgaaaacgat gatggcgcac gcgagaaata
    10741 ttctgctggt cgccgatcac actaagtatc atgcttcggc ggcggttgaa attggtaacg
    10801 tggcacaggt cactgcgctc tttaccgacg agctgccgcc cgctgcgcta aaatcacgct
    10861 tacaagacag ccaaattgaa atcattcttc cccaggaaga cgcgtagatt tttgtgaccg
    10921 ttaacctggc ttcatacctt gccacatagc caaacccatc ctttcccgct acagttaatt
    10981 tcttgtggcg cgaaaggagg caaaaatgct ctatatcttt gatttaggta atgtgattgt
    11041 cgatatcgac tttaaccgtg tgctgggagc ctggagcgat ttaacgcgta ttccgctggc
    11101 atcgcttaag aagagttttc atatggggga ggcgtttcat cagcatgagc gtggggaaat
    11161 tagcgacgaa gcgttcgcag aggcgctgtg tcatgagatg gctctaccgc taagctacga
    11221 gcagttctct cacggctggc aggcggtgtt tgttgcgctg cgcccggaag tgatcgccat
    11281 catgcataaa ctgcgtgagc aggggcatcg cgtggtggtg ctttccaata ccaaccgcct
    11341 gcataccacc ttctggccgg aagaataccc ggaaattcgt gatgctgctg accatatcta
    11401 tctgtcgcaa gatctgggga tgcgcaaacc tgaagcacga atttaccagc atgttttgca
    11461 ggcggaaggt ttttcaccca gcgatacggt ctttttcgac gataacgccg ataatataga
    11521 aggagctaat cagctaggca ttaccagtat tctggtgaaa gataaaacca ccatcccgga
    11581 ctatttcgcg aaggtgttat gctaaaaacc attcaggaca aagccaggca ccgtacccgt
    11641 ccactatggg cctggctaaa actactctgg caacgtattg atgaggacaa catgacaacc
    11701 ctggcaggta accttgccta tgtgtcgttg ctctcattag tgccgctggt tgccgttgtt
    11761 tttgcgcttt tcgccgcttt tcccatgttt tccgacgtca gcattcagtt acgtcacttt
    11821 atttttgcca actttctgcc tgctactggc gatgttattc agcggtatat cgaacaattt
    11881 gttgctaatt ccaacaagat gaccgccgtt ggggcgtgcg ggctgatcgt caccgcgtta
    11941 ttgttgatgt actccatcga tagcgcgttg aataccatct ggcgcagtaa acgagcgcga
    12001 ccaaaaattt actcgttcgc cgtgtactgg atgattttaa cgctggggcc gctgctggca
    12061 ggggccagtc tggcgatcag ttcctatttg ctctccctgc gctgggcgag cgatctcaat
    12121 actgtcatcg acaacgtgct gcgtattttt ccgttgctgt tgtcgtggat ctccttctgg
    12181 ttgctgtaca gcattgttcc taccatccgc gtacctaacc gcgacgcgat tgtcggcgcg
    12241 tttgtcgccg cactcctgtt cgaagcagga aagaaaggtt tcgcgcttta tatcaccatg
    12301 ttcccgtcat atcagctcat ttacggtgtg ctggcggtga tccccattct ctttgtttgg
    12361 gtctactgga catggtgtat cgtcttgctt ggcgcggaaa ttactgtcac tctcggggaa
    12421 taccgcaaac taaaacaagc agctgaacaa gaagaagacg acgaaccatg attgcattaa
    12481 ttcaacgcgt aacccgtgcc agcgtcaccg tggagggaga agtgacgggc gaaattggcg
    12541 cgggactttt ggtgttattg ggtgtcgaaa aggatgacga cgaacagaaa gcaaaccgtc
    12601 tgtgcgagcg tgtgctcggc taccgcatct ttagcgatgc cgaaggcaag atgaatctca
    12661 acgtgcaaca ggcgggcggc agtgtactgg tggtttccca gtttaccctc gccgcagata
    12721 ccgaacgggg gatgcgccca agtttctcca aaggcgcatc accggatcgc gcagaggcgt
    12781 tatatgacta tttcgtcgaa cgctgccgcc agcaagagat gaacacgcaa acaggacgct
    12841 tcgctgccga tatgcaggta tcgctggtca atgatggccc cgtgacattc tggttgcagg
    12901 tatgagccag cttccagggt tgtcacggga aacaagagag agtatcgcta tgtatcacct
    12961 tcaggttcca caaacagaag aagagttaga gcgttactat cactttcgct gggaaatgtt
    13021 gcgtaagccc ctgcatcaac caaaaggttc ggaacgcgac gcgtgggatg cgatggcgca
    13081 tcaccagatg gtcgtcgacg agcagggtaa tctggtggcg gtaggccgat tgtatattaa
    13141 tgccgacaat gaagcgtcca ttcgctttat ggtcgttcac ccggacgtgc aggacaaagg
    13201 gttaggaacg ctgatggcga tgaccctgga gtcggtggcg cgtcaggaag gcgttaagcg
    13261 cgtgacctgt agcgcccgtg aagacgcggt ggagtttttc gccaagctgg ggtttgttaa
    13321 tcagggagaa atcaccacgc caaccaccac gccgattcgc cattttttga tgattaagcc
    13381 cgtcgccact ctggatgata ttttgcatcg cggcgactgg tgcgcgcagc tgcaacaggc
    13441 gtggtacgaa catatcccgc ttagtgaaaa aatgggc
//
LOCUS       DAJTYM010000097        13441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTYM010000097 DAJTYM010000000
VERSION     DAJTYM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            185..2032
                     /gene="ldtD"
                     /locus_tag="OO575_003119"
     CDS             185..2032
                     /gene="ldtD"
                     /locus_tag="OO575_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6665988.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2213..2761
                     /locus_tag="OO575_003120"
     CDS             2213..2761
                     /locus_tag="OO575_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6665989.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2788..3435
                     /gene="gloC"
                     /locus_tag="OO575_003121"
     CDS             2788..3435
                     /gene="gloC"
                     /locus_tag="OO575_003121"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6665990.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3658..4848)
                     /gene="aspC"
                     /locus_tag="OO575_003122"
     CDS             complement(3658..4848)
                     /gene="aspC"
                     /locus_tag="OO575_003122"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6665991.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5033..6121)
                     /gene="ompF"
                     /locus_tag="OO575_003123"
     CDS             complement(5033..6121)
                     /gene="ompF"
                     /locus_tag="OO575_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6665992.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6722..8122)
                     /gene="asnS"
                     /locus_tag="OO575_003124"
     CDS             complement(6722..8122)
                     /gene="asnS"
                     /locus_tag="OO575_003124"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6665993.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8291..9493)
                     /gene="pncB"
                     /locus_tag="OO575_003125"
     CDS             complement(8291..9493)
                     /gene="pncB"
                     /locus_tag="OO575_003125"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6665994.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9759..12374
                     /gene="pepN"
                     /locus_tag="OO575_003126"
     CDS             9759..12374
                     /gene="pepN"
                     /locus_tag="OO575_003126"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6665995.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12581..13348)
                     /gene="ssuB"
                     /locus_tag="OO575_003127"
     CDS             complement(12581..13348)
                     /gene="ssuB"
                     /locus_tag="OO575_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6665996.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13345..>13441)
                     /gene="ssuC"
                     /locus_tag="OO575_003128"
     CDS             complement(13345..>13441)
                     /gene="ssuC"
                     /locus_tag="OO575_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="part of the ABC type transport system for
                     alkanesulfonate SsuABC; SsuB the ATP-binding subunit and
                     SsuC the permease; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6665997.1"
                     /translation="LLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3397 a         3408 c         3402 g         3234 t
ORIGIN      
        1 cgcgcttttc tttaaacttc tttacattag gttatgtaaa aacgtatcgg cgtttatata
       61 ctgaagataa gcctgatgag taacaggctt gctcgtcata ctttcgtgag tattggcgtt
      121 gtacaggcaa gtcgtaaaat aacagcctgg ctattcagag tatgataaaa acagggggca
      181 agggatgttg cttaatatga tgtgtggtcg tcggctgtcg gcaatcagtt tgtgcctggc
      241 cgtaacattc gctccactgt tcaatgcgca ggccgatgag cctgaagtaa tccctggcga
      301 cagcccggtg gctgtcagtg aacagggcga ggcactgccg caggcgcaag ccacggcaat
      361 aatggcgggg atccagccat tgcctgaagg tgcggcagaa aaagcccgca cgcaaatcga
      421 atctcaatta cccgcaggtt acaagccggt ttatcttaac cagcttcaac tgttgtatgc
      481 cgcacgcgat atgcaaccca tgtgggaaaa ccgtgatgct gttaaagcct tccagcaaca
      541 gctggcagag gtggcgattg ctggtttcca gccgcagttt aataaatggg tagagttact
      601 gaccgatcct ggtgttaacg ggatggcacg tgacgtggtg ctctctgatg cgatgatggg
      661 ctatctccat ttcattgcaa atattccgat caaaggcact cgctggctat atagcagtaa
      721 accttatgcg ctttcaacgc cgccgctttc ggtgattaac caatggcagc tagcgctgga
      781 taaaggtcaa ttgcctacgt ttgttgcagg actggcaccg cagcatccgc aatatgcggc
      841 gatgcatgaa tcgttactgg tcttactcag tgacaccaaa ccgtggcccc aactgaccgg
      901 caaagcaacg ttgcgcccag ggcagtggag taacgacgta ccggcgttgc gcgaaatatt
      961 gcaacgcaca ggcatgttgg acggggggcc gaaaattact ctacctggcg atgacacgcc
     1021 aactgacgcg gtagtcagcc catccgctgt tactgttgaa acagcagaaa ctaagccgat
     1081 ggacaagcaa acgacgtctc gtagtaaacc tgcgcctgcc gttcgtgccg cctacgataa
     1141 tgaactggtg gaagccgtta aacgttttca ggcatggcaa ggattgggag cagatggtgc
     1201 tattggcccg gcaacgcgtg actggttaaa cgtaacgccc gcccagcgtg ctggcgtgtt
     1261 ggcactcaac atccagcgat tgcgcttgct gccaacagag ctttctaccg ggatcatggt
     1321 taacattcca gcctattcgc tgctctacta tcagaacggc aatcaggtgc tggattcgcg
     1381 agtcattgtc ggtcgccccg atcgcaaaac gccgatgatg agcagtgcac ttaacaacgt
     1441 agtggtaaac ccgccgtgga acgtaccacc aactctggca cgcaaagata ttctgccgaa
     1501 agtgcgcaac gatccgggat atctcgaaag ccatggctat acggtgatgc gcggctggaa
     1561 cagcagagaa gcgattgacc catgggaggt tgactggtct acaatcacgg cctcgaattt
     1621 accgtttcgc ttccagcagg ctccaggccc acggaactcg ctggggcgct ataaattcaa
     1681 tatgccgagt tcagaggcca tttatttgca tgacacgccg aaccacaatc tgttcaagcg
     1741 tgatacacgc gcattgagct caggctgtgt acgagtgaat aaagcttccg atctggcgaa
     1801 tatgctgttg caggatgcag gatggaatga caaacgtatt tctgatgcgc tgaagcaggg
     1861 tgatacacgt tacgtcaata ttcggcagtc gattccggtg aatctctact acctgacggc
     1921 ctttgttggt gcagatgatc gtacccagta tcgtacagat atttacaatt atgatctgcc
     1981 tgcgcgatcc agctcgcaaa tcgtatcgaa agcggaacaa ttaatcaggt aaatgaagta
     2041 gttcggggaa atcaattgtc gtaataatta gcatgattgg gggcgattct ctgcagcccc
     2101 cgtcactgct ggggttgagt catcttgacg tctgctttac gggcggttaa ggtgcctctt
     2161 gtgcgccaga agtgcatata aacgataaca ttgagctgta gacttgatta tcatggacaa
     2221 attcgacgct aatcgccgca aattgctggc gcttggtggc gttgcactcg gtgccgccat
     2281 cctgccgacc cctgcgtttg caacactctc taccccacgc ccgcgcattt tgacactcaa
     2341 taatcttcat accggagagt caatcaaagc ggagtttttc gatggcagag gctatattca
     2401 ggaagaattg gcaaaactta accatttttt ccgcgattac cgcgcgaaca aaataaagtc
     2461 catcgaccca ggattattcg accagttgta tcgcctgcaa gggttgttag gcacgcgcaa
     2521 accggtgcaa ctcatttccg gttatcgttc cattgatacc aacaatgaac tacgcgcccg
     2581 cagccgtgga gtagcgaaga aaagctatca cactaaaggc caggcgatgg atttccatat
     2641 tgaaggtatc gcgttaagca atattcgcaa agccgcgtta tctatgcgcg caggtggtgt
     2701 aggatattac ccacgtagta actttgtgca tattgatacc gggccagcac ggcactggta
     2761 gtaatcgctt aacgaaacag gggcagtatg aactatcgta ttattccggt caccgcattc
     2821 tcccagaact gttcattgat ctggtgtgaa caaacccgcc tggccgcact ggtcgatcct
     2881 ggcggcgatg cggaaaaaat caaacaggaa gttgatgaca gcggcctgac actgatgcag
     2941 atcctgctga cgcatggtca tctggaccac gttggcgcag cggcggaact ggcgcaacat
     3001 tacggcgtgc cggtttttgg cccggaaaaa gaagatgagt tctggctgca aggcttgcct
     3061 gcgcaaagtc gtatgtttgg tctggaagag tgccagccgc tgacgccaga tcgttggctg
     3121 aacgaaggcg ataccatcag catagggaat gtgactttac aggtgttaca ttgccctggg
     3181 catacgccgg gtcatgtcgt gttttttgat gatcgggcaa agctgctgat ttctggcgat
     3241 gttattttca aaggcggagt agggcgcagt gacttcccgc gtggcgatca taatcaactg
     3301 atttcttcaa tcaaagataa attgctgcca ctgggggatg acgtgacatt tattccgggt
     3361 cacggaccat tatctacact tggttatgag cgcctgcaca atcccttcct gcaagacgaa
     3421 atgcccgtct ggtaaaggcg cataaaaaag tccactttta atgcgggcct ggatttctgg
     3481 caaagtgcgc tttgtttatg ccggatgcgg cacgagcgcc ttatccggcc tacaaaatcg
     3541 tgcaaattca aaatattgca ggggacgcgt aggcctgata agcgtagcgc atcaggcaat
     3601 gttgcgtttg tcatcagtct cagcccgctt ttcagcgggc ttcattgttt ttaacgctta
     3661 cagcactgcc acaatcgatt cgcacagcgg agccatgtta tctggtgtca tcccggccac
     3721 atttacgcga ccagaagcaa cagcatatac gccaaactct tcgcgcagac gcagcacttg
     3781 ttcttttgtc agaccactga aggagaacat gccgttctgt ttgatgataa agctgaagtc
     3841 gcggtttgca cctttttcct gcagcgtatt gacgaacaac tgacgcatac gctgaatacg
     3901 ctggcgcata tcagtcagct cttgttccca aatcgcacgt aatgcatcgc tgctcaggat
     3961 ggtggcaaca acagaagcgc cgtgtgctgg agggttagag tagttagcgc gaatcgccgc
     4021 tttcatttgg ctgaatgcgc gatcaacgat ttcactgtcg gcagcaacca gagtacaagc
     4081 gccaacacgc tcgttgtaca ggccaaagtt tttagagtag gaactggcaa caatcagctc
     4141 tttatgcata gccgcgaaag cgcgcagtcc ttcagcatct tcttccagac cacgggcaaa
     4201 accctggtaa gcgaagtcaa acagcggtaa ccagcctttc tcaacggaga gttgtgccag
     4261 tgtctgccat tgttccagcg tagggtcgat accggttggg ttatggcagc agccatggaa
     4321 cagcactacg tcgccagcct gagcttcgtt cagactgtta atcagtgcat cgaagtcaag
     4381 ggtgtgattt tccgcatcat aataagcgta ttcacgaact tccagacctg cagagttaaa
     4441 gacgctctta tggttcggcc agcttgggtt gctcacccac acacgcttaa cgctggtatt
     4501 ttttgccagg aagtcggcag ccacgcgtag tgcgccagtg ccacccggag tctgtgccgt
     4561 gcgagcacgt ttgtcattga tcagggcgct acctttacca aacagcagtt cctgagtgca
     4621 gcgaccaaat tcaaggatgc cgtcaatgcc gaggtaattt ttggtggttt cattttcgag
     4681 cagatactgt tcagcctttt tcacgctggt cagtaccggg gttttgcccg tctcatcttt
     4741 atagacacca atcccgaggt taattttgcc gggacgttca tcggcacgaa acagatcggc
     4801 caggcccaga atcgggtcgg caggagcggc ggtaatgttc tcaaacatga cgaggttcca
     4861 ttatggttac agaagggaag tccgctatca gggtaacggg agatttacaa aattccaact
     4921 attactgatg aaaacgcagg ctgtttttgc aagacgtgag attgctctgg aaggcataaa
     4981 aaaaacagga ccaacgtcct gttttttcgg catttaacaa agaggtgtgc tattagaact
     5041 ggtaaacgat acccacagca acggtgtcgt ctgaacctac gcccagtttg ttgtcagaat
     5101 cgatctggtt gatgatgtag tcaacatagg tggacatgtt tttgttgaag tagtaggttg
     5161 cgcccacttc aaagtagttc accagatcaa catcaccgat accttctacg tctttcgctt
     5221 tagatttggt gtaagcgatg gacggacgca gaccgaaatc gaactggtat tgcgcaacta
     5281 acagaacgtc ttgcgttttg ttggcgaagc cgctggtgtt tgtaaattta ttagtgatcg
     5341 gcgtagcgtt acgggtttca ccgtagttcg ctgccaggta gatgttgttc gcgtcgtact
     5401 tcagaccagt agcccactgt tcagcttttt taccgttgcc aagaggttga gcttcttgca
     5461 ggttggtacg gtcagctgca ccataagcac caacgatacc aaagccttcg tattcgtagc
     5521 tgatagaacc gccaacaccg tcgccgttag aacggcgtgc agtgtcacgc tcgtttttac
     5581 ccaggtactg aacagcgaag ttcaggccat caaccagacc aaagaagttg gagttacgat
     5641 aggtagcaac gccgccaaca cgaccaacga agaagtcatc gctgtatgca gtatcaccac
     5701 caaattctgg cagcatatcg gtgtaaccca gtgcatcata aaccacaccg tagttacggc
     5761 cgtaatcgaa agaaccaacg tcagcgtatt taagacccgc gaatgccaga cgcgttttgt
     5821 taccagtttg agcgtcagcg ccttcagagt tgttaccctg gaagttatat tcccactgac
     5881 cataaccggt cagatcggaa ttgatttgag tttccccttt aaaaccaaga cgggcatagg
     5941 tcatgtcgcc attgccaccg taactgtttt caccgttacc cttggaaaaa taatgcagac
     6001 caacagtttt accgtacaga tctactttgt tgccatcttt gttatagatt tctgcagcgt
     6061 ttgcagtacc tgctactaac agagcaggga cgatcactgc cagaatattg cgcttcatca
     6121 ttatttatta ccctcatggt ttttttatga cacctgccac tgccgtcaat aagttctgtc
     6181 aataaaaatt tacggaacta ttgatgagag tttggtgtct ttatgtgtct gcaggcatct
     6241 ttccattcaa actaacgttt cgctaccgtg aaagtgctac aaagataaag atttagtttc
     6301 aaaaagaaaa aatatgtaac caaaagtaaa aattaaggag ctttgtgaac accgtcatat
     6361 ttccatagga acgtgatgat atttacagca atttttatct atttatatga tttacttata
     6421 tttaaattaa ctaaacggaa attttgtttc tgatggaaac tttatcgacc tggcacaaaa
     6481 tcctctttca gacatccaga atgccaaaaa atagtatgaa aattgtgcta tcagctaaga
     6541 aaaaactaat ccgcaataag agtacgggtt attttttgtg cgtaaatgtt gcaatgtaat
     6601 attccgtaac aggatgatcg ttatggcggg ggtgcaaata gattgcttga cttaacaaac
     6661 agataacttg acagaaagga taaaaaagag ccagcaggcg ctggctcttt gaggaagaaa
     6721 atcagaagct ggcgttgcgc ggagtacgtg ggaacggaat cacatcacgc acgttttgca
     6781 cgccagttac gtaagcaatc aggcgttcaa aaccaagacc gaaacctgaa tgcggaacag
     6841 taccgtagcg acgcagatcg cgataccacc agtaatcttc tttattcagg cccatttcca
     6901 gcatacgctc gtccagaacg tccagacgtt cttcacgctg ggagccacca atgatctcac
     6961 cgatgcccgg agccagaacg tccatagccg caacggtttt accgtcttcg ttgaggcgca
     7021 tatagaacgc tttaatatct ttcggatagt ttttaaccac taccggtgct ttaaagtgtt
     7081 cttccgccag ataacgctca tgctcagaag agagatcgac gccccagtaa accgggtttt
     7141 caaacttcct gccgcagttt tcgagaatgg tcactgcgtc ggtgtaatcc acctgcgcga
     7201 aatcggcttc aatgaagcgt tccagacgtg aaacggcatc tttatctacg cgttcagcga
     7261 agaatttcat gtcgtctgcg cgttcttcga gaaccgcttt gaagacatat ttcagcatgg
     7321 cttcagccag acccgcgata tcgttcaggt tagcaaacgc cacttccggc tccagcatcc
     7381 agaattccgc caggtgacgg ctggtgttgg agttttcagc acggaaagtc gggccgaagg
     7441 tataaatttt ggataatgcg caagcgtagg tttcgccgtt caactggcca gatacggtca
     7501 ggaaagactc tttaccaaag aagtctttgt cgaaatccac tttgccctga tcgttacgcg
     7561 gcaggttttc cagatccagc gtagaaacgc ggaacatttc gcctgcacct tcggtatcag
     7621 atgcggtaat cagtggcgtt gaaacccaga agaatccctg ctcgttaaag aagcgatgca
     7681 gcgcctgcgc cagcgtatgg cgaacgcgcg cgacggcacc aatcaggttt gtgcgcggac
     7741 gcaggtgagc gacttcacgc agatactcaa tgctgtggcg ttttgccgcc atcgggtaag
     7801 tgtctggatc ttcaacccaa ccagcaactt caaccttgct ggcctgaatt tcaaattgtt
     7861 gcccctggcc cggcgatgcc acgactttac ccgtcacaat gaccgagcag ccggtggtca
     7921 gacgcaggac gtcttcattg taattgggca gagaattatt gatgacagcc tgtacaggat
     7981 caaagcagga accgtcataa acggcgagga aggagatgcc agcttttgaa tctcggcggg
     8041 tacgtaccca tccgcgtacg gtgacttcgc tgtcaacggc tacgcggccc tggagtacgt
     8101 cggctacagg cacaacgctc ataatattct ctctgttaat agtcggaaaa aataaacact
     8161 tgtccacccg taaatggggg ggatacctat gttacctggc atctgcaatc agacaagcag
     8221 aattcgcaaa tgcagcgaaa gatttcggaa ttaaaggtaa aaaagggagc cgattagctc
     8281 cctgatgata ttaactggct tttttaatat gcggaaggtc gaacgctttg cgcagcgccc
     8341 gaacaaacgc tttatcatgg cagatagttt tgccagggct gtcagaaagt ttcgccaccg
     8401 gtttaccgtt acactctacc aacttaatca caatattcag gggttttacc tgggggatat
     8461 cgcaggtcag gcgagtccca ataccaaaac ttaattgcac gcgggaagag aagtggcggt
     8521 atagctcaac cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt ttactctgtg
     8581 gatcaattcc cagcttttca taatgtgcaa tggctttttc accccattca accgggtcgc
     8641 cagagtcatg acgcaggccc tgataccgac tagcgaactc gacaccgaaa tcacgcagga
     8701 aagcatccat agtgatgcag tcggttaatg caatgccaag ttggtcggga tactcttcca
     8761 gccaggcagc aagtgcagct cgctggctgt tggctagatc cgggctgatt tgctgatgtg
     8821 cctggaacca ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga cgcgccagat
     8881 cgtagttgct ggtgcccacg aaccaggatt cctgttgcag acgcttaacg atggtttctt
     8941 gtacttcgcg agaaaaacgg cgacgggtgc caaaatccat cagatggaag cgcgacatat
     9001 caagaccggc ggttaacgcc gagaagtcga ctaatttgct ttccagcgtg tcgagggctt
     9061 gcgcaacgtc ggcctgcggt gagcgatagc gatgtaccat ttcactgata accgccagca
     9121 aaggaacttc ccagaggatg acttcacgcc acggaccgct taagcgaata tccagcttgc
     9181 cattatcgtt ggacacggtg acttgttccg ggttaaagcg gaactcgcgt aaccagttaa
     9241 gatagtcggc cttaaagaaa ggcagggcag agagccactg atattcatca tcctgcaggc
     9301 gcaggtgctg catcgcctga atctgttcac gaatagcatc ggcataaata cccagcagat
     9361 cgtcacctcg gcaacgaaac tccgccgcga catgcacatc gtaatagtga tgaaacacgg
     9421 cttgctgcat atgcaactta taagcatctg tatccagcaa cgagtgcaga acaggagaag
     9481 cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt acaataaaca
     9541 tcttcaggaa ctgctggagt ataccttgtt tcgcaattta ttgaaccccg atcacaccat
     9601 atgccacctt tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt agcaataaag
     9661 gcgtttgtac ctgaaaagat gaagattctg catagcgcga tttacgcaac aggaatagac
     9721 tgaacaccag actctataaa agatgctaaa ggttatttat gactcaacag ccacaagcca
     9781 aataccgtca cgattatcgt gcgccggatt accagattac tgatattgac ttgacctttg
     9841 acctcgacgc gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt catggtgcat
     9901 cagatgctcc gcttcgtctc aacggcgaag acctcaaact ggtttctgtt catattaatg
     9961 atgagccgtg gaccgcctgg aaagaagaag aagagggcgc actggtcatc agtaatttgc
    10021 cggagcgttt tacgcttaag atcattaatg aaataagcct ggcggcgaat acggcgctgg
    10081 aagggcttta tcagtcaggc gatgcgcttt gcacccagtg tgaagccgaa ggtttccgcc
    10141 atattacgta ttatctcgac cgcccggacg tgctggcgcg ttttaccacc aaaattattg
    10201 ccgataaaat caaatatccc ttcctgcttt ccaatggtaa ccgcgttgcg caaggcgaac
    10261 tcgaaaacgg acgccattgg gtacagtggc aggacccgtt cccgaaaccg tgctacctgt
    10321 ttgcgctggt ggcaggcgac tttgatgtac tgcgcgatac ctttaccacg cgttctggtc
    10381 gcgaagtagc actggagctg tacgtcgatc gcggcaacct tgatcgcgcg ccgtgggcga
    10441 tgacctcgct gaaaaactcc atgaaatggg atgaagaacg ctttggcctg gagtatgacc
    10501 tcgacatcta tatgatcgtc gcggtggatt tcttcaatat gggcgcaatg gagaataagg
    10561 gtctgaatat ctttaactcc aaatatgtgc tggcccgcac cgacaccgcc accgacaaag
    10621 attacctcga tattgaacgc gttatcggcc atgaatattt ccataactgg accggtaacc
    10681 gagtgacctg ccgcgactgg ttccagctca gcctgaaaga aggtttaacc gtcttccgcg
    10741 atcaggaatt cagctctgac cttggttccc gcgcagttaa ccgcatcaat aatgtgcgca
    10801 ccatgcgcgg attgcagttt gcggaagacg ccagcccgat ggcgcaccca atccgcccgg
    10861 acatggtcat tgagatgaac aacttctaca ccctgaccgt ttacgagaag ggcgcggaag
    10921 tgatccgtat gatccacacc ctgctgggcg aagaaaactt ccagaaaggg atgcaactct
    10981 atttcgagcg tcatgatggt agcgcagcga cctgtgacga ctttgtgcag gcgatggaag
    11041 atgcgtcgaa tgttgatctc tcccatttcc gccgttggta cagccagtcc ggtacgccgg
    11101 ttgtgaccgt caaagacgac tacaatccgg aaactgagca gtacaccctg accatcagcc
    11161 agcgcacgcc agccacgccg gatcaggcag aaaaacagcc gctgcatatt ccgtttgcca
    11221 tcgaattgta tgacaacgaa ggtaaagtga tcccgttgca gaaaggcggt catccggtga
    11281 attccgtgct gaacgtcacc caggcggaac agacctttgt ttttgataat gtctacttcc
    11341 agccggtgcc tgcgctgctg tgcgaattct ctgcgccagt gaaactggaa tataagtgga
    11401 gcgatcagca actgaccttc ctgatgcgtc atgcgcgtaa tggtttctcc cgttgggatg
    11461 cggcgcaaag tttgctggca acctacatca agctgaacgt cgcccgtcat cagcaagggc
    11521 agccgctgtc tctgccggta catgtggctg acgctttccg cgcggtgctg ctcgatgaga
    11581 agattgatcc ggcgctggcg gcagaaatcc tgacgctgcc ttctgtcaat gaaatggctg
    11641 aactgttcga tatcatagac ccgattgcta ttgccgaagt acgcgaagca ctcactcgta
    11701 ctctggcgac tgaactggcg gatgagctgc tggctattta caacgcgaat taccagagcg
    11761 agtaccgtgt tgagcatgaa gatattgcaa aacgcactct gcgtaatgcc tgcctgcgct
    11821 tccttgcttt tggtgaaacg catctggctg acgtgctggt gagcaagcaa taccatgaag
    11881 caaacaatat gaccgatgcg ctggcagcgc tttctgcggc ggttgctgca cagctgcctt
    11941 gccgtgacgc gctgatgcag gagtacgacg ataagtggca tcaggacggt ctggtgatgg
    12001 ataaatggtt tatcctgcaa gccaccagcc cggcggcgaa tgtgctggag acggtgcgtg
    12061 gtctgttgca gcatcgctca tttaccatga gcaacccgaa ccgtattcgt tcgttgattg
    12121 gtgcgtttgc gggcagcaac ccggcagcgt tccatgccga agatggcagc ggttaccagt
    12181 tcctggtgga aatgcttacc gacctcaaca gccgtaaccc gcaggtagct tcacgtctga
    12241 ttgaaccgct gattcgcctg aaacgttatg atgccaaacg tcaggagaaa atgcgcgcgg
    12301 cgctggaaca gttgaaaggg ctggaaaatc tctctggcga tctttacgag aagataacta
    12361 aagcactggc ttgatacttt tgagccaaaa tgcctgatgc gacgcttgac gcgtcttatc
    12421 aggcctacaa ctccgttgaa attgctggtt ttgtaggctg gataaggcgt tcacgccgca
    12481 tccggcagcc gtgcccaact gcctgatgcg atgcttgacg tgtcttatca ggcctacaag
    12541 tccgttgaaa atgctggttt tgtgcgccac atccggcata ttaaccatga cgccgaatca
    12601 gttgttcaga atgcccacgc cgcattaccc gttgcaatac ttccgcttcc agctccgcta
    12661 atcgcaccga ccctaatcga cgcggacggg ggatatccac tgtcaaatcc agactaattt
    12721 ttccctcttc aattaacaac acccggtcag ccattgctac cgcttcgctc acatcatgcg
    12781 tcaccaacag tacggtaaag ccgtgctgct gccaaagtga cacaatcaaa tcctgcatct
    12841 cgagtcgcgt taaggcgtcc agtgccccga gcggttcatc aagcaacaat aaacccggtc
    12901 gatgaatcaa cgctcttgcc agcgccacac gctgtttctg cccgccagaa agtgcggcag
    12961 gccattcccc ggcgcgattc tccagtcctg ccgcagccaa cgcccgacgc gcggcatcgc
    13021 gccactgacc tttaaggcct aacccaacgt tatcaatcac cgatttccac ggcagcagac
    13081 gcgcatcctg aaacatcatt cgcgtatcat cctgaatttc agccagcggt gtggtgcccg
    13141 ctaacacatc gcctgcggtt ggcgtttcca gaccggccag caggtgcagc agggtacttt
    13201 taccaccacc gctgcggccc accaccgcca caaactgacc tgccggaata tgtaaatcca
    13261 gttggttcag gacgatattt tccgcgtaat gtttgcttac tgcattgagc aacaatggtg
    13321 tgccctggtt cagacgagca gtattcatat cgtggcctcc ttcaaatgat aagccgggtt
    13381 ccagcgcaac cagaggcgct ctaacagctg cgcgctgacg tcagcaagtt tgccgagcag
    13441 g
//
LOCUS       DAJTYM010000098        12871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTYM010000098 DAJTYM010000000
VERSION     DAJTYM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..2672
                     /locus_tag="OO575_003129"
                     /pseudo
     CDS             1..2672
                     /locus_tag="OO575_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2682..6328
                     /locus_tag="OO575_003130"
                     /pseudo
     CDS             2682..6328
                     /locus_tag="OO575_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6389..6796
                     /locus_tag="OO575_003131"
     CDS             6389..6796
                     /locus_tag="OO575_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6665998.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7338..8426
                     /locus_tag="OO575_003132"
     CDS             7338..8426
                     /locus_tag="OO575_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR6665999.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8437..10716
                     /locus_tag="OO575_003133"
     CDS             8437..10716
                     /locus_tag="OO575_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666000.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10709..11845
                     /locus_tag="OO575_003134"
     CDS             10709..11845
                     /locus_tag="OO575_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6666001.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            11842..>12871
                     /locus_tag="OO575_003135"
     CDS             11842..>12871
                     /locus_tag="OO575_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666002.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLG"
BASE COUNT         3203 a         3400 c         3288 g         2980 t
ORIGIN      
        1 agccaccgta ttacaggagc aaggtgttgc agccctgccc cgatttgctc cctatgccgc
       61 aagtgactac tgcgccgatg tgttgcgtca tatcaatcat ccgttcgcat taacactgct
      121 tatacgtgta gccgggcata ctaaacgctg tcacgatcga atgacgaaag cctgcgctgc
      181 gttcccacac gcagcactgg cagcactggc ggaacttcta gtgcaaaaag aagagaatag
      241 ttggcgcatt atgctaatga cgatgtttat ctcgcaacca gcactggcag aacaggtcat
      301 tccctggctt tcgacacccg cagttgcagt gctgaaatca tgccagcacc aactgacaca
      361 gccctcaaac catgccagcg ccgatctact gccagccata gtagtctccc caccatggat
      421 ttcgaaaaag aaaaaatcgc cgattccggt gctggattta gcgccattag gcatcgagcc
      481 aatctgttat ctgacagaag aaatcagtaa tcaacttttg gcgaaatata tctggtattc
      541 aaaacacatc acggttagcc atgaagaaag tactaccaac ctgttggcaa ggatgggttt
      601 tcaacgacgg atcgctggta catatattaa agctcccgaa gcggtagttg aggcatggct
      661 aaatgaagat tattcaacct tactaagtga atttaaggtg tttcattcac ctaccgggca
      721 ttattggcag ttggggattt tgacaacatt gccgctggag gctggagaaa gcagtaaaag
      781 catggaatgc ccttactcta tctccacata ccgataccga atacgccatg ttacattttg
      841 gactcaaagg tttacctggt ttagttaact caattacacg ctatccacag gaagccttgc
      901 ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc cgtcgcccgt gccttcaaca
      961 aactcaaaac gctacgcgaa aacgcccgca cctggctgtt gaaatacccg gaacatgccc
     1021 ttaccggcct gctgcctgcg gcgctcggca aagccggtga agcacaggat aacgcccgcg
     1081 ctgccttgcg tatgcttacc gaaaacggtc atcagccatt actgcaagaa atcgcccggc
     1141 gttataacca gtcggaagta acagacgcgg tgaacgctct gcttgcgctc gatcccttag
     1201 ataatcaccc gacaaaaatc cccactcttc cgacctttta tcagccatcg atctggacgc
     1261 gcccggtatt aaaagcaaac gcccaatcac tgccagataa cgccctcctc cacctcggcg
     1321 aaatgctccg cttccctcag gaagaggctc tgtatccggg attattgcag gtgaaagacg
     1381 cctgtaccgc tgactctctg gctgaatttg cctgggatct gtttaccgcc tggcagaccg
     1441 ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt aggcgttctc ggtaacgatg
     1501 acaccgcccg caaactgacg ccattaatac gcgcctggcc tggtgaatcc cagcataaac
     1561 gcgccaccgt tgggttggat attctcgccg ctatcggtag tgatatcgcc ctgatgcagc
     1621 ttaacggcat tgctcagaaa ctgaaattca aagcattaca ggagcgggca aaagaaaaga
     1681 ttgccgacat tgccgagagc cgcgaactca cggtagcgga gcttgaagat cggttagcac
     1741 cggatctcgg tctggatgat aacggttcgt tgctgctgga ttttggccca cgccagttca
     1801 ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga tgtttccggc agccgcctga
     1861 aagacctgcc aaaaccaaac aaaagcgatt atgagtcaca ggcgaacgat gctgttaacc
     1921 gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgcggc ccagcaggta gcaaggcttg
     1981 aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa cttccaactc ttcctggttg
     2041 tgcatccgct ggttcgccac ttaacccgcc gtctgatttg gggcgtttat agcgccgaca
     2101 accagctact ggcctgtttc cgggtagcgg aagataacag ctacagcacc gctgacgatg
     2161 atcttttcac cctgccggaa ggcgatatct ctatcggcat tcctcacgtt ctggaaatat
     2221 caccgacgga tgctgtcgcc tttggtcagc tttttgccga ctacgaactg ctaccaccgt
     2281 tccgtcagct tgaccgtaac agttacgccc tgacagaagc cgagcgcaac gccagcgaac
     2341 tgacccgctg ggcaggcaga aaatgcccga gcggccgggt catggggctg gcaaataaag
     2401 gctggataaa gggcgaacca caggatggag gctggatgat caaacctctc ggtcgctggt
     2461 cgttaatcat ggaaatcgac gaaggttttg cggtgggtat atcaccagcc gaacttagcg
     2521 ctgagcagct cttaagcaag ctgtggctat gggaaggcaa agcggaaagc tatggctggg
     2581 ggagtaattc aacacaggaa gcgcagttct ccgtactcga tgccatcacc gccagcgagc
     2641 taattaacga tattgaagcc ctgtttgaat aaggaaagag catggacaag gaattaccgt
     2701 ggctggcgga taacgcccaa ctggaactga aatataaaaa aggcaaaaca ccactcagtc
     2761 atcgcaattg gccgggcgaa ccagtgcctg ttatcactga aagcatcatt cagacactgg
     2821 gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat tgtctggcgt tatgaaaatt
     2881 tttcaccgga gtggcagtcc gccattacgc aggccatcaa cttgatcggc gaacacaaac
     2941 cctcagtccc ggcccgcaca atggcggcac tagcctttat cgcgcaaaat gacaaccaac
     3001 agttgctcga cgaaatcgtc caacaagagg ggctggaata tgcgactgag gtggtgattg
     3061 cacgccagtt tattgcacgg tgttatgaga gtgatcctct ggtagtcaca ttgcagtatc
     3121 agaacgagga ctatggctat ggttatcgct cagaaaccta taacgaattc gatctccgac
     3181 tgcgtaaaca cctctctctg gcagaggaaa gctgctggca gcgttgcgcc gacaaactca
     3241 ttgccgcact gccaggaata accaaagttc gccgcccttt tattgcgctg atcctccccg
     3301 aaaaaccaga aatcgccaat gagttggtaa gccttgaatg cccgcggact cattttcatt
     3361 ctaaggagtg gttaaaagtt gttgctactg accccaaagc ggtgagaaaa ctcgaacgct
     3421 actggagcca ggatatattt agcgatcgag aagccagcta catgtcgcat gaaaaccact
     3481 tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg actggcagtc gttccgcgcc
     3541 tcgcgatata tgcccataaa gaagattgcg gcagtctgct ggtacaaatt aaccatccgc
     3601 aagtcatccg caccttgctg ttagttgctg ataaaaacaa acccagcctg caacgtgtgg
     3661 ctaaatacag taaaaacttc ccccatgcga cactcgccgc actggcagaa ctgctggcgt
     3721 taaaagaacc accagcccgc ccaggttatc caatcatcga agacaaaaag ctgcctgcac
     3781 agcaaaaagc tcgagatgaa tactggcgta cgctgttaca aacgctgatg gcatcgcagc
     3841 cacaactggc agcagaagtg atgccgtggt taagtactca agcccgggca gtgctgaata
     3901 gttatttatc ggcaccgccc aaaccagtta ttgatagcac cgataacagc caaatgcctg
     3961 aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa gacttccata cgtcactttt
     4021 cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc gccgaataaa cactggacgc
     4081 cggaacagca aactgcaatt gaccatttta gcgccatgtc ttttgacgaa cgtcttgcta
     4141 cgagaggagc ggatatcttc ttgcgggaat taggttttga cgttcatagc ctggaattta
     4201 ataagcgtct tcttaatgga cagttggatg cctttgatgc tcagcaactg caagaataca
     4261 catcaatagg actattgcgt acttatcacg cgcatcaggc caaatctaaa cagtaccggg
     4321 aagacgccac caatgcatta ctggcacaag atagcattgc gttgatggga gggtggcgaa
     4381 cttttcagca cggattttac tatggtaatg aacagcgttg ggggatttgg aatctgtacc
     4441 ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg gcaacaaatt gtcgaggctg
     4501 attttcatta caccggggtg gaatatttgt taagtgtgct gggtaccgat gctttgcctg
     4561 gattaaacac cgcctttgcc cgacatccaa aagagatatt tccgctgctg attcatttcg
     4621 gcgcaacaga actggccctg cccattgccc gcgtctggca cctttttgcg ggccagcgta
     4681 atctggctcg ccagtggatt ttacaatggc cggaacatac ggctactgca cttatccctc
     4741 ttgtcttcac caaatctagc gataaaagcg aagctgcatt acttgccctg cgtttacttt
     4801 acgaacatgg gcatggcgaa tggctacaaa ctgtagccaa tcgttggcag cgtacagatc
     4861 tatggcctgc cctggaacaa ttacttaaac agagtccaat agagatttac ccggcacgca
     4921 ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc caggccgcgc cttatcacta
     4981 ataatcaacc tgttaccgat gacgctctgg aaattatcgg cgaaatgctg cgctttaccc
     5041 aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc
     5101 tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag
     5161 acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca cgggatctaa
     5221 cgacacatat cctcgcctgg ccacaagaag gcaaatctgc ccgtgcggtc agtggcatga
     5281 acatccttac cctgatgaat aatgatatgg cgctgatgca gctgcatcat atatcgcaac
     5341 gggcgaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggta gtcgcagaaa
     5401 atcgcgggct aagccaggaa gagttagcgg acagattagt cccaacactg ggccttaatg
     5461 atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa
     5521 acctcaaccc ggttatcttt gatcagcaaa acgttcgcca gaaaagtgtt ccccggttgc
     5581 gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggttaaaaa
     5641 aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca
     5701 cccgacgctg gtcgctggca gattttcatt ctctgtttgt taatcatccc tttacccgtc
     5761 tggttaccca gcgattaata tggggggtta tccggcaaat gaaccgcgtc gtttactcaa
     5821 cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg caagatgagc caattgacct
     5881 gcctgcggac gctctgattg gcattgccca cccgttagaa atgacagcag aaatgcgcag
     5941 tgaatttgca cagctttttg ccgattacga aattatgccg ccttttcgcc agttgtcgcg
     6001 ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac agcctgactc gctgggaagg
     6061 taaatccgct accgttgggc aacttatggg aatgcgatac aaaggctggg agtcaggtta
     6121 tgaggacgca tttgtctatg acctgggtga gtaccggctg gtccttaagt tttcacccgg
     6181 ttttaaccac tacaatgttg atagcaaagc gctaatgagc ttccgttctc ttcgagtgta
     6241 ccgtgacaat aaatccgtca cttttgccga acttgatgtg tttgatttga gtgaggcgtt
     6301 aagcgcacct gacgtcattt tccattaaaa caaagcgggc agtgatgcga ctgcccgtta
     6361 tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa aaagagctgg ttgctattta
     6421 cgattatgag gtccctgtac ctgaagatcc gttttccttc agacttgaga tccataaatg
     6481 ctctgaatta tttacaggtt ccgtctaccg actggaacgg ttccggctac gtccaacatt
     6541 tcatcaacgt gatcgagagg atgttgaccc gctaataaat gatgcgttga tttatataag
     6601 agatgagttt attgatgagc aaaaattacg aggcgaatca cctgaacctg taatagtaat
     6661 ttttaattgt aaactacaga atatattcaa ccaacaaata gaattatata ctctagataa
     6721 ttcaagtcgc agcacatgca acttgaagta tgacgagtat agctttatta aaggggggaa
     6781 cggtattccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc aatcaaccgt
     6841 aacagagcca ctaatagctc cagtacagca atgagcacgg ttaacttttg tgtcagcgtg
     6901 tccatccgct ctccttatta aggagcacaa caccagcacc ttacccttac actgcctgtc
     6961 gatactggtt tacgtttgtt gagtcgccat gttgcactcc ggatcagagc actacctgcg
     7021 gcaccacccg tccggtagcc aggacttgaa ataggttcga tccggcgcaa cttcccaaca
     7081 gtcaggatta taaatctcat tatcttaatt gcaacactgt ccagaggtgg ttataattcg
     7141 ttcgttggaa cacgatgttg cacaggcagt ggtgttaggc ctgaaaatag taaataaaag
     7201 aacctgcctc accagcagtt ttttttattt actgtgatct gctttccaga tatttttcgc
     7261 tcaaacaact aatgcgccaa acatttattg cgcgtaaaat atcgtttatt tcattaatac
     7321 atttcaggga tgaatatatg tcaccgcaga ataatcatct gcagcgtccg cctgctgctg
     7381 tgttatacgc cgatgaactg gcaaaattaa aacaaaatga taacgcacct tgcccgcccg
     7441 gttggcagtt aagtttgcct gcggcccgtg cttttatcct tggcgatagc gcgcaaaata
     7501 ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg tatgttagtc actcttgcca
     7561 ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc aaaatctctc ctgtctgaat
     7621 tactggcaac ctctattagc ggcgatgccg ggttaaccat ccagggcggc gcatctacta
     7681 ccgaagatca aatcaagtac ggctggaact acgccctgct tatcaatcat ggtccatcaa
     7741 cggaagcgct ggtccctgcc ccgctttacc agggaatgcg cgacggcaaa atcgtccgtt
     7801 ttgaagagat aacgcgtaca ccgctggaag tgcaagactg tctgttaggt atgctttctg
     7861 acagggtgat gacggtgccg gaactcactg gtgaagccag tcagctctat gcgcgagaag
     7921 gctttaatat tattgccact gccaataccc gcgatcgcgg cgtcaatgaa atgagtgctg
     7981 cgctaaagcg tcgcttcgat tttgaaactg tctttccgat tatggatttc gcccaggagc
     8041 tggagcttgt cgccagtgct tcggcgcgtt tgctggccca tagcggtatt ccgcataaag
     8101 tgcctgacgc ggtactggag ttactggtcc gcaccttccg cgatctgcgt gccaacggcg
     8161 aaaagaaaac gtcaatggat acactaacag cgattatgtc caccgctgaa gccgtgaacg
     8221 tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa tcgcgcaggt gagccagccg
     8281 acctggtgga ctgcattgcc ggaaccatcg tcaaagataa cgaagaagat cgcgctcgtc
     8341 tgcgccgtta cttcgaacag cgtgtcgcca cacataaaga agctcactgg caggcttatt
     8401 atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga gcgagccgtt aattgtcggc
     8461 atccggcatc atagtccggc ctgcgcccgg ctggtgaaat cgttaatcga aagccagcgg
     8521 ccacgatacg tgttgattga aggccctgct gattttaatg accgggtaga cgaactgttt
     8581 ttagcccacc agcttccggt agctatttac agttattgcc agtatcagga cggtgcagcc
     8641 cccgggcgtg gtgcctggac gccatttgct gaattttcgc cggagtggca ggcgctacaa
     8701 gccgatcgtc gtattcaggc acaaacttac ttcatcgatt tgccttgctg ggcgcagagt
     8761 gaagaagagg acgattcgcc tgatacgcaa gaggaaagcc agaccttgct gctgcgtgcc
     8821 acccgcatgg ataacagcga caccctgtgg gatcacttgt tcgaagatga aagccagcaa
     8881 actgcattac cctctgcact ggcgcactat tttgcccaac tgcggggcga ctcccccggc
     8941 gatgctctaa atcgtcagcg cgaagccttt atggcccgct ggattggatg ggcgatgcag
     9001 caaaataatg gcgacgtgtt agttgtctgc ggtggctggc acgctccggc actggcaaaa
     9061 atgtggcgcg aatgtccgca ggtaattaac acgccagaat tgccgtcgct ggcagatgcc
     9121 gttacaggtt gttatctcac cccctacagt gaaaagcgcc ttgatgtgct ggcaggatac
     9181 ctttcaggaa tgcctgcccc ggtctggcaa aactggtgct ggcagtgggg cttacagcag
     9241 gcgggagaac aactgctaaa aacggttctc acccgtttgc gccagcacaa gttgcctgct
     9301 tcgaccgcgg atatggctgc cgctcatctg catgcgatgg cactggcaca gttgcgcggt
     9361 catacactac cgttacgcac tgactggctg gatgccatag caggatcgct gattaaagaa
     9421 gccctgaatg cgccgttgcc gtggagctat cgcggcgtta ttcatcccga taccgatccg
     9481 attctgctaa cgttgataga cacattagcg ggtgacggat tcggtaaact tgccccttct
     9541 acgccacaac cgcctctgcc aaaagatgtc acttgcgaac tggaacgtac cgcaatctct
     9601 cttccggcgg agcttacctt aaatcgcttt aaccccaatg gactagcgca aagtcaggtg
     9661 ttacatcggc tggcaatact ggagatccca gggattgtac gccagcaggg aagcacactg
     9721 acacttgcag gcaacggtga agaacgctgg aaattaaccc gcccgcttag ccagcatgcg
     9781 gcattgattg aggccgcctg ctttggtgcc acactcctgg aagccgcacg ccataaatta
     9841 gaagccgata tgctggacgc gggtggaatc ggcagcatta ccacatgtct tagccaagcg
     9901 gcgtcagcgg gtctggcgtc cttcagtcaa caattactgg agcaactcac attattaatc
     9961 gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg aagtgctata tgccttatgg
    10021 cggctggatg aaattagcgg tatgcaaggc gcgcagatat tacaaacgac gttatgcgcg
    10081 gctatcgatc gcacgctgtg gctgtgtgaa tccaacggca gaccggatga aaaggagttt
    10141 cacgctcact tgcatagctg gcaagcgttg tgtcatatcc tgcgcgatct acatagcggt
    10201 gtgcaactcc ccggcgtttc gctttctgcg gcggtagcct tgctggagcg acacagtcag
    10261 gcgattcatg tcctggcact ggatcgcggc gcgactcttg gtgcgctgat gcgtctggaa
    10321 catcccaacg ctagtgccga agcggcgctg acgatgctgg cgcagttatc cccggcacaa
    10381 tccggcgagg cgctgcacgg tttgctggca ttagcccgtc atcaactggc ctgtcagccg
    10441 gcatttatcg ccggtttcag cagtcattta aatcaactaa gtgatgccga ttttaccaat
    10501 gccctgcccg atttacgcgc ggcaatggcc tggctaccgc cacgagaacg cgggacgctg
    10561 gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc ccgtttcggc actgcaaatg
    10621 ccgttgcatt gtccaccgca agccattgca catcatcaac aactcgaaca gcaggcactg
    10681 gcatcgctgc aacactgggg agttttccat gtctgaactg aacgatcttc tgaccacccg
    10741 tgagctacaa cgctggcgat taattcttgg cgaagcggca gaaacgacgc tttgtgggct
    10801 ggatgacaac gcccggcaga tagatcacgc gctggagtgg ctgtatgggc gcgatcctga
    10861 acggctccag cgtggtgaac gctctagtgg attaggtggc tcaaatctca ccactcctga
    10921 gtggatcaac agtattcaca cgctgtttcc gcaacaggtg attgagcggc tggaaagcga
    10981 tgccgtactg cgctacggca ttgaagatgt ggtgacaaat ctcgacgtgc tggaacgtat
    11041 gcagccttcc gaaagcctgc tacgcgccgt tttgcacacc aaacatctga tgaaccccga
    11101 agtactggct gccgcccgca ggattgtgcg ccaggttgtg gaagaaatta tggcgcgact
    11161 ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat cgtcgtcgcc gctcatttat
    11221 tccactggcg cgaaactttg atttcaaaag tactctgcgc gccaacctgc aacactggca
    11281 cccgcaacac ggcaagttgt atatcgaatt cccccgcttt aacagccgca ttaaacgcca
    11341 aagcgagcaa tggcaactgg tcttactggt tgatcaaagc ggatcgatgg tcgattcggt
    11401 gatccactct gcggtgatgg cggcctgttt atggcagtta cccggcattc gtacccatct
    11461 ggtggcgttt gacacaagcg tcgttgatct cacggcagac gttgccgatc cggtagagtt
    11521 attaatgaaa gtgcaattag gcggcggaac caatatcgcc agtgccatgg agtatggtcg
    11581 gcaacttatt gaacaacctg caaaaagcgt cattatcctc gtgagcgatt tttatgaagg
    11641 gggttcatca tcattgctga cgcaccaagt gaaaaagtgt gtccagagcg gcatcaaagt
    11701 gctgggactg gcagcgctcg atagcacagc aacaccttgc tatgaccgcg atacggccca
    11761 ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca ccgggcgagc tggcatcatg
    11821 gcttgcggag aatcttcagt catgaattca ctacgtccgg aattattaga actgacaccg
    11881 caggcactga cggcgttaag caatgccggt tttgttaagc gcagccttaa ggaactggaa
    11941 aatggcaacg tcccggaaat cagccatgag aacagcgctt taatcgccac cttcagtgac
    12001 ggtgtccgta cccagctggc gaacggccag gcactgaaag aggctcagtg cacctatggg
    12061 gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa gttatcaacg actttgtgct
    12121 accgcccagc ctacgggaaa agaagaggaa tgggatccgg caatctggct ggaagaactg
    12181 gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc tggtcgctaa aagcatcacc
    12241 attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc gcttaccgat gagcgatgtg
    12301 cgtttttatt cccgcagcag tattcgtttc gcccgttgtg attgtattga aggcacactt
    12361 tgcgaacatg tcgtactggc agtacaggcc tttgtccagg caaaagcgca gcaagcggaa
    12421 ttgactcatt taatctggca gatgcgcagc gaacacgtca catcatctga cgatccgttt
    12481 gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac aactaagcca ggcattatgg
    12541 ctgagcggca tcagccagcc gcttattcac tacgaggccg ctttcagccg cgcgcagcaa
    12601 gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc tacggcaact gcgcgccagc
    12661 gttgatgcct tccacgcccg cgccagccac tatcatgccg gagaatgctt acgtcagctt
    12721 gcggcattaa acagtcgatt aaattgcgca caagagatgg cccggagcga cagtgttggt
    12781 gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg gcattgccgg agaagcaaag
    12841 cttgatcatc tgcggctggt gtctttaggt a
//
LOCUS       DAJTYM010000099        12524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTYM010000099 DAJTYM010000000
VERSION     DAJTYM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..539)
                     /locus_tag="OO575_003136"
                     /pseudo
     CDS             complement(1..539)
                     /locus_tag="OO575_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
     gene            complement(799..1419)
                     /gene="sodA"
                     /locus_tag="OO575_003137"
     CDS             complement(799..1419)
                     /gene="sodA"
                     /locus_tag="OO575_003137"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6666003.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1704..2738
                     /gene="rhaT"
                     /locus_tag="OO575_003138"
                     /pseudo
     CDS             1704..2738
                     /gene="rhaT"
                     /locus_tag="OO575_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(2735..3601)
                     /gene="rhaR"
                     /locus_tag="OO575_003139"
     CDS             complement(2735..3601)
                     /gene="rhaR"
                     /locus_tag="OO575_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6666004.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(3657..4493)
                     /gene="rhaS"
                     /locus_tag="OO575_003140"
     CDS             complement(3657..4493)
                     /gene="rhaS"
                     /locus_tag="OO575_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6666005.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4781..6250
                     /gene="rhaB"
                     /locus_tag="OO575_003141"
     CDS             4781..6250
                     /gene="rhaB"
                     /locus_tag="OO575_003141"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6666006.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6247..7506
                     /gene="rhaA"
                     /locus_tag="OO575_003142"
     CDS             6247..7506
                     /gene="rhaA"
                     /locus_tag="OO575_003142"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6666007.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            7748..8572
                     /gene="rhaD"
                     /locus_tag="OO575_003143"
                     /pseudo
     CDS             7748..8572
                     /gene="rhaD"
                     /locus_tag="OO575_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            8582..8896
                     /gene="rhaM"
                     /locus_tag="OO575_003144"
     CDS             8582..8896
                     /gene="rhaM"
                     /locus_tag="OO575_003144"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6666008.1"
                     /translation="MIRKTFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9197..9643
                     /locus_tag="OO575_003145"
     CDS             9197..9643
                     /locus_tag="OO575_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6666009.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            9654..11105
                     /locus_tag="OO575_003146"
     CDS             9654..11105
                     /locus_tag="OO575_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6666010.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11095..12165
                     /locus_tag="OO575_003147"
     CDS             11095..12165
                     /locus_tag="OO575_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6666011.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12165..>12524
                     /locus_tag="OO575_003148"
     CDS             12165..>12524
                     /locus_tag="OO575_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR6666012.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYE"
BASE COUNT         2972 a         3255 c         3319 g         2978 t
ORIGIN      
        1 caccaggaac ggtaatactg cgccgacgaa aacatgcggt tcaaacgagg caatcccggc
       61 agtgcccaga ataatcatcg tcatgagcgg cccggactcc aacgacatca acacaaatgc
      121 ccagcttctt ctttcgtacc gtactgctgc atgatggaag cgtaaagtcc gccgttggtc
      181 atatccatcg ccgccaccag cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca
      241 ccatgttccg gaataatgcg cgaggcaatt gccgcaacca cccacgcgac ggcaatttta
      301 gttaccacca gcgtaccgga tttacgcagt accgttcccg tcgcgcttaa ttttattgac
      361 gcccccatgc aaaaaaacca caccgccaga atgggcaccg taccggtaat catcccgttg
      421 gtgaatgatc caaaatattt ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg
      481 aatagcggga cgagcatcat cccccccggg attttctcaa tcgagcgttt tatctgcatt
      541 tccatcacct gtcggcatat ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt
      601 gttgatggaa acgaagatag acattcattt aatcaataaa agtgatcaaa atcacatcaa
      661 ttgaaacgct gttttatttt tataggacca ttgtttttat tttggaattg atcacaaaaa
      721 aaccaccgcc gttggcgatg gttcattttt taagctgata tgcggcctat acgcctcatt
      781 gcagcaggcg gcaaatgatt attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt
      841 caccacgttc cagaactctt tgatgtagtc cggacggcgg ttctggaatt tcaggtagta
      901 agcatgttcc cacacatcca ggcccagaat cgggaagccg gaagcgccag aaatagcttc
      961 acccatcagc ggagaatcct ggttagcagt agaaaccacc gccagtttat cgcctttcag
     1021 caccagccat gcccagccgg aaccaaagcg ggaagctgcc gctttttcaa attctgcttt
     1081 gaagttatca acggagccga agtcacgttc gatagccgct ttcaggtcac cctgcagggt
     1141 ggtgcctttt ttcagacctt tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt
     1201 gcgcagtacg gttttcttgt ctgctggcag ctggtccagt ttggtgatca gctcttcaac
     1261 cggcaggttg gcaaattctg gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt
     1321 ctgatggtgt ttggtgtggt ggatttccat ggtctgctta tcgaagtgcg gttccagggc
     1381 atcgtaagca tacggcaggg atggcagggt atagctcata ttcatctcca gtattgtcgg
     1441 gcggccgatt gttaatgccg cgtaagcagt tggttcatta tagttaatta aatgatattg
     1501 aaaatgatta tcaatgccgt acttttcgta agggtatggt tttgcaggaa aatgcccgag
     1561 atgtgaagca aatcacccac ttaatgccgt gattgccagt aaatcgacaa cggcggcaac
     1621 aggcgaaagg ttaatcgaca gcacgatttt tacactcatc tcgtcggaga tgtgaagcga
     1681 cgaaaaatga tgaggataag aagatgagta acgcgattac gatggggata ttttggcatt
     1741 tgatcggcgc ggccagtgca gcctgttttt acgctccgtt caaaaaagta aaaaaatggt
     1801 catgggaaac catgtggtca gtcggtggga ttgtttcgtg gattattctg ccgtgggcca
     1861 tcagcgccct gttactaccg gatttctggg cgtattacag ctcgtttagt ctctctacgc
     1921 tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg taatatcaac tatggcctga
     1981 ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc cattggcatt acgttgattg
     2041 tcggtacgct gatgacgcca attatcaacg gcaatttcga tgtgctgatt aacaccgaag
     2101 gcggacgcat gacgttactc ggcgttctgg tggcgctgat tggcgtaggg attgtaactc
     2161 gagccgggca gttgaaagag cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa
     2221 aagggctggt gctggcggtg atgtgcggca ttttctctgc cgggatgtcc tttgcgatga
     2281 acgccgcaaa accgatgcat gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg
     2341 ctctgccaag ctatgttgtc atcatgggcg gcggcgcgat cattaacctc ggtttctgtt
     2401 ttattcgtct ggcaaaagtg aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac
     2461 cgctgatcat tcacaatgtg ttactctcgg cactgggcgg gttgatgtgg tatctgcaat
     2521 tctttttcta tgcctagggc cacgcccgca ttccggcgca gtatgactac atcagttgga
     2581 tgctgcatat gagtttctat gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt
     2641 ggaacaatac aggacgccgt ccggtaacgg tgttgagcct cggttgtgtg gtgattattg
     2701 tcgccgctaa catcgtcggc atcggcatgg cgaattaatc tttctgcgaa ttgagatgac
     2761 gccactggct gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa tagttactat
     2821 cttcaaagcc acattcggtc gaaatatcac tgattaacag gcggctatgc tggagaagat
     2881 attgcgcatg acacactctg acctgccgca gatattgatt gatggtcatt ccagtctgct
     2941 ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa aatttatcca
     3001 gcgcaaaggg acttttcagg ctagccgcca gccgggtaat cagcttatcc agcaacgttt
     3061 cgctggatgt tggcggcaac gaatcactgg tgtaacgatg gcgattcagc aacatcacca
     3121 actgcccgaa cagcaactca gccatttcgt tagcaaacgg cacatgctga ctactttcat
     3181 gctcaagctg accgataacc tgccgcgcct gcgccatccc cacgctacct aagcgccagt
     3241 gtggttgccc tgcgctggcg ctaaatcccg gaatcgctcc ctgccagtca agattcagct
     3301 tcagccgctc cgggcaataa ataatattct gcaaaaccag atcgttaacg gaagcgtagg
     3361 agtgtttatc gtcagcatga atataaaaga gatcgccacg ggtaatgcga taaggacgat
     3421 cgttgagtac atgcagacca ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg
     3481 tatgttcggc aaagacatct tgcggataac ggtcagccac agcgactgcc tgctggtcgc
     3541 tggcaaaaaa atcatctttg agaagtttta actgatgcgc caccgtggct acctcggcca
     3601 gagaacgaag ttgattattc gcaatatggc gtacaaatac gttgagaaga ttcgcgttat
     3661 tgcagaaagc catcccgtcc ctggcgaata tcacgcggtg accagttaaa ctctcggcga
     3721 aaaagcgtcg aaaagtggtt actgtcgctg aatccacagc gataggcgat gtcagtgacg
     3781 ctggcctcgc tgtggcgtag tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat
     3841 cgctgaggcg tcagtcccgt ttgctgctta agctgccgat gtagcgtacg cagtgaaaga
     3901 gaaaattgat ccgccacggc atcccaattc acctcatcgg caaaatggtc ctccagccag
     3961 gccagaagca agttgagacg tgatgcgctg ttttccaggt tctcctgcaa actgctttta
     4021 cgcagcaaga gcagtaattg cataaacaag atctcgcgac tggcggtcga gggtaaatca
     4081 ttttcccctt cctgctgttc catctgtgca accagctgtc gcacctgctg caatacgctg
     4141 tggttaacgc gccagtgaga cggatactgc ccatccagct cttgtggcag caactgattc
     4201 agcccggcga gaaactgaaa tcgatccggc gagcgataca gcacattggt cagacacaga
     4261 ttatcggtat gttcatacag atgccgatca tgatcgcgta cgaaacagac cgtgccaccg
     4321 gtgatggtat agggctgccc attaaacaca tgaatacccg tgccatgttc gacaatcaca
     4381 atttcatgaa aatcatgatg atgttcagga aaatccgcct gcgggagccg gggttctatc
     4441 gccacggacg cgttacaaga cggaaaaaaa tccacactat gtaatacggt catactggcc
     4501 tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt caggttctta ccttaaattt
     4561 tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa
     4621 atgcggtgag catcacatca ccacaattca gcaaattgtg aacatcatca cgttcatctt
     4681 tccctggttg ccaatggccc attttcctgt cagtaacgag aaggtcgcga attcaggcgc
     4741 tttttagact ggtcgtaatg aaattcagca ggatcacatt atgacctttc gcaattgtgt
     4801 cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg
     4861 ccgcagcctg acgctgcgcg aaatccatcg ttttaacaat gggctgcata gccaaaacgg
     4921 ttatgtcacc tgggatgtgg atagcctgga aagtgccatt cgccttggat taaacaaggt
     4981 gtgcgaggaa gggattcgta tcgatagcat tgggattgat acctggggcg tggactttgt
     5041 gctgctcgac caacagggtc agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac
     5101 caatggcctg atgccgcagg cacaacagca actcggcaaa cgcgatattt atcagcgtag
     5161 cggcatccag tttctaccct tcaatacgct ttatcagttg cgtgcgctga cggagcaaca
     5221 acctgaactt attccacaca ttgctcacgc tctgctgatg ccggattact tcagttatcg
     5281 cctgaccggc aagatgaact gggaatatac caatgccacc accacacaac tggtcaatat
     5341 caatagcgac gactgggacg agtcgctact ggcgtggagc ggggccaaca aagcctggtt
     5401 tggtcgcccg acgcatccgg gtaatgtcat tgggcactgg atttgcccgc agggtaatga
     5461 gattccggtg gtcgccgttg ccagccatga taccgccagc gcggttatcg cctcgccgtt
     5521 aaacggttca cgcgccgctt atctctcttc tggcacctgg tcattgatgg gcttcgaaag
     5581 ccagacgcca tttaccaatg acacggcgct ggcagccaac atcaccaatg aaggcggggc
     5641 ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta tggctgcttc agcgagtgct
     5701 tcaggaacgg caaatcaacg atctcccggc gcttatcgcc gcgacacagg cacttccggc
     5761 ctgccgcttc atcatcaatc ccaatgacga tcgctttatt aatcctgacg agatgtgcag
     5821 cgaaattcag gctgcgtgtc gggaaacggc gcaaccgatc ccgggaagtg atgctgaact
     5881 ggcgcgctgt attttcgaca gtctggcgct gctgtatgcc gatgtgttgc atgagctggc
     5941 gcagctgcgc ggtgaagatt tctcgcaact gcatattgtc ggcggcggct gccagaacac
     6001 gctgctcaac cagctatgcg ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga
     6061 agcctcaacg ctcggcaata tcggcatcga gttaatgacg ctggatgaac tcaacaatgt
     6121 ggatgatttc cgtcaggtcg tcagcaccac cgcgaatctg accaccttta cccctaatcc
     6181 tgacagtgaa attgcccact atgtggcgca gattcactct acacgacaga caaaggagct
     6241 ttgcgcatga ccactcaact ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg
     6301 gtggggattg atgtcgagga ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac
     6361 tgctggcagg gcgatgatgt ttccggtttt gaaaacccgg aaggttcgct gaccgggggg
     6421 attcaggcca caggcaatta tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat
     6481 ctggaacagg ctatgcggct gattccgggg ccgaaacggc ttaatttaca tgccatctat
     6541 ctggaatcag atacgccagt ctcgcgcgac cagatcaaac cagagcactt caaaaactgg
     6601 gttgaatggg cgaaagccaa tcagctcggt ctggatttta acccctcctg cttttcgcat
     6661 ccgctaagcg ccgatggctt tacgctctcg catcccgacg acagcattcg ccagttctgg
     6721 attgatcact gcaaagccag ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca
     6781 ccatcggtga tgaacatctg gatcccggat ggtatgaaag atatcaccgt tgaccgtctc
     6841 gccccgcgtc agcgtctgct ggcagcactg gatgaggtga tcagcgagaa gctaaaccct
     6901 gcgcaccata tcgacgccgt tgagagcaaa ttgtttggca ttggcgcaga gagctacacg
     6961 gttggctcca atgagtttta catggggtat gccaccagcc gccagactgc gctgtgcctg
     7021 gacgccgggc acttccaccc gactgaagtg atttccgaca agatttccgc cgccatgctg
     7081 tatgtaccgc agttgctgct gcacgtcagc cgtccggttc gctgggacag cgatcacgta
     7141 gtgctgctgg atgatgaaac ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt
     7201 gaccgggtgc atatcggcct tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg
     7261 gtcattggta cacgcaatat gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct
     7321 gagctgcgca agctggaagc ggcgggcgat tacactgcgc gtctggcact gctggaagag
     7381 cagaaatcgt tgccgcggca ggcggtctgg gaaatgtatt gccaacatca cgatacgcca
     7441 gcaggtagcg aatggctgga gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt
     7501 gggtaaacac tgccggatgc ggcacgagcg ccttatccgg cctacgggtc gaatgtccat
     7561 gcgggctgtc accctcaccc cggccctctc cctgaaaggg cgagggagaa aaacatagcc
     7621 tcgtgcggca acggagctga acaccgctcc ggacagtccc ctcgccccta cggggagagg
     7681 gttagggtga ggggaacaag ccgcaccatg tctaaacaag gcaacctaat ttacaggaac
     7741 acagaacatg caaaacatta cccagtcctg gtttgtccag ggaatgatca aagccaccac
     7801 cgatgcctag ctgaaaggct gggacgagcg caacggcggc aacctgacgc tgcgcctgga
     7861 tgacgccgat atcgcgcttt atcacgacaa cttccaccca caaccgcgct atattccgct
     7921 cagccaaccc atgcctttac tggcaaatac gccgtttatt gtcaccggct ccggcaaatt
     7981 cttccgtaac gtccagctcg atcctgcagc taacttaggc gtcgtaaaag tcgacagcga
     8041 tggcgcgggc taccatattc tctgggggtt aaccaacgaa gccgttccaa cttctgaact
     8101 tccggctcac tttctctccc actgcgagcg cattaaagcc accaacggta aagatcgggt
     8161 gatcatgcac tgccacgcca ccaacctgat cgccctcacc tatgtactgg aaaacgacac
     8221 cgcggtcttt actcgccaac tgtgggaagg cagcaccgaa tgtctggtag tattcccgga
     8281 tggcgttggc attctgccgt ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc
     8341 acaagagatg caaaaacatt cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg
     8401 accgacgctg gatgaagcct ttggtttaat cgacaccgca gaaaaatcag cacaagtatt
     8461 agtgaaggtt tattcgatgg gcggcatgaa acagaccatc agccgtgaag agttgatagc
     8521 gctcggcaag cgcttcggcg ttacaccact agccagtgcg ctggcgctgt aaggagcaaa
     8581 catgatccgc aaaacctttg tcatgcaggt aaaccccgac gcccacgaag agtatcagcg
     8641 tcggcataat cccatctggc cggaactgga agcagtgttg aaatctcacg gtgtgcataa
     8701 ctacgccatt tacctcgaca aagcgcgtaa tctgctgttt gccatggtag agattgaatc
     8761 tgaagaacgg tggaatgcgg tagccagtac tgatgtttgc caacgttggt ggaaatatat
     8821 gaccgatgtt atgcccgcta acccagataa cagcccggtg agtagcgagc tgcaagaagt
     8881 gttttacctg ccgtaattgc ccaatgtgcc ggagatgcct ccggcacaat attttaaatt
     8941 tcgatttgaa aaattaaaca tattacatct cccggtaagt tatatttccc tgatacattg
     9001 tgagtaaatc acaaaaataa tgaataaccc attaatgatt catgtggttt atttaaataa
     9061 ccattatgtg cattactccg caaatctgac ctttcactct gtctcaaatt gtcattgttt
     9121 accgacatat cgcctctcat cacttaatcg ctccaccatt acaattgatt aaaatatatt
     9181 ttatggagtc cgattaatgg cagctcttac tgcaagctgt attgacctga atattcaggg
     9241 caatggcgct tattccgttc tgaagcagtt ggcgacaata gcgttacaaa acggttttat
     9301 caccgactca caccagttcc tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg
     9361 atttggttcc ggtgtcgcag tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt
     9421 attattcgcc cgcaaagcgc aggctattga ctggaaagcc agcgatggcg aagacgtcaa
     9481 ttgctggatc tgcctcggcg tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg
     9541 cacactgtgt cgcaaaatta ttcaccagga atttattcat caactgcaac agggcgatac
     9601 cgaccaggtg ctggccttgt taaatcaaac cctcagctca taaggaagtg gcgatggagt
     9661 catccttacg tattgtcgcg atcaccaact gccccgccgg gatcgctcac acctacatgg
     9721 tggcggaagc cctggaacag aaagcccgtt ctctcggcca taccataaaa gtggaaactc
     9781 aagggtccag tggcgttgaa aaccgcttat ccagcgaaga gattgccgct gccgattacg
     9841 tcattcttgc taccgggcgt ggcctgagcg gtgatgatcg cgcgcggttt gccgggaaga
     9901 aagtttatga gattgccatc tccctggcgt tgaaaaatat cgaccagatt ttcagcgaat
     9961 taccgacaaa ctcgcagctt tttgccgcag atagcggcgt gaagcttggc aaacaggaag
    10021 tgcaaagcgg cagcgtgatg agccacctga tggcgggcgt ctctgcggcg ctgccgtttg
    10081 tcatcggcgg tggtatcctg gtggcgcttg ccaacatgct ggtgcagttc ggtttgccgt
    10141 ataccgatat gtcgaaaggc gcgccgtcgt ttacctgggt agttgaatcc atcggttacc
    10201 ttggcttcac ctttatgatc cccatcatgg gcgcttatat tgcctcgtcg attgccgata
    10261 aaccggcttt cgctccggcg tttctggttt gctatctggc gaacgacaaa gcgctgctcg
    10321 gcacccagtc gggtgcggga ttcctcggcg cggtggtgct ggggctggcg attggctatt
    10381 tcgtcttctg gttccgcaaa gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa
    10441 tgctgatccc gttcgttact ctgctggttt tcggcgtgct cacttactac gttatcgggc
    10501 cggtgatgtc cgacctcatg ggcgggctgc tccacttcct gaataccatt cctccatcaa
    10561 tgaagtttgc ggcagcgttt ctggtcggtg cgatgctggc gttcgatatg ggcggcccca
    10621 tcaataaaac cgcctggttc ttctgcttct cactgctgga aaaacacatt tatgactggt
    10681 acgccatcgt cggcgttgtc gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca
    10741 ttgcgcccaa actgtttacc cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg
    10801 gtgccacagt cgcaaccgaa ccggctatac cttacgctct ggcagctccg ttgccgatga
    10861 ttaccgccaa tacgctggcg ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca
    10921 aacgtctggc acctggtttg ggtatctttg acccgctgat cggcctgatg tcgccggtag
    10981 gttcgtttta tctggtgctg gcgattggcc tcgcgctgaa catttcattc attatcgtcc
    11041 tgaaaggatt gtggttacgc cgcaaagcga aagccgcgca acaggagctt gtccatgaac
    11101 attgagttac tgcaacagtt gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt
    11161 cgcgacatcc tgataaacac gctggaacct tgcgttaatg aaatcacctt tgatggtctg
    11221 ggcagctttg ttgcccgtaa gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg
    11281 gatgaagtcg gctttatggt cacccacatc gacgagagcg gttttctgcg tttcaccacc
    11341 attggtggct ggtggaatca gtcgatgctc aaccatcggg taaccatacg cacacacaag
    11401 ggagtgaaaa tccctggtgt gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa
    11461 aggcaacagc cgctgtcatt tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat
    11521 gaggtggaaa aacgcggtgt ggcgatgggc gattttatta gcccggaagc caattttgcc
    11581 tgctggggcg aagataaagt ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg
    11641 atggctgaac tattgcagac ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc
    11701 gtggaagaag aagttgggct acgcggggca caaacctcgg cggaacacat taaaccggat
    11761 gtagtgatcg tgctggatac cgctgtcgca ggcgatgttc cgggcattga taacattaaa
    11821 tacccgctga aactgggcca ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc
    11881 aaccagaaac tggtagcagc gttaaaaaac tgtgccgcac ataacgattt accgctgcaa
    11941 ttttccacca tgaaaaccgg agcgacggat ggcgggcgct acaacgtgat gggcggcggg
    12001 cgtccggttg ttgcgctgtg cctgccaacc cgctatctgc acgctaacag cggcatgatt
    12061 tcaaaagccg attacgatgc tctgctcacg ttgatacggg gttttctgac gaccttaacc
    12121 gcggagaaag tcaacgcgtt tagccagttc cgtcaggtgg attaatgcta aacgaacgcc
    12181 agttaaagat tgtcgatctg ctggagcaac agccgcgcac gcctggtgag ctggcgcaac
    12241 agactggcgt ttcaggcagg accatcctgc gtgatattga ctatctcaac tttaccctta
    12301 acggcaaagc ccgcattttc gccagtggca gtgcgggcta tcagctggaa atcttcgagc
    12361 gccgcagctt ttttcagttg ctgcaaaagc acgataacga cgatcggctg ctggcgctgt
    12421 tattactgaa tactttcact ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa
    12481 cgtgggtagc agagcgtctg ccccagttaa aacagcgtta tgaa
//
LOCUS       DAJTYM010000100        12444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTYM010000100 DAJTYM010000000
VERSION     DAJTYM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1051)
                     /locus_tag="OO575_003149"
     CDS             complement(join(<1..802,802..1051))
                     /locus_tag="OO575_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HCR6666013.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAIT"
     gene            complement(1127..3466)
                     /locus_tag="OO575_003150"
                     /pseudo
     CDS             complement(1127..3466)
                     /locus_tag="OO575_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(3400..4023)
                     /locus_tag="OO575_003151"
     CDS             complement(3400..4023)
                     /locus_tag="OO575_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6666014.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(4020..5708)
                     /locus_tag="OO575_003152"
                     /pseudo
     CDS             complement(4020..5708)
                     /locus_tag="OO575_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(5857..8484)
                     /gene="gyrA"
                     /locus_tag="OO575_003153"
     CDS             complement(5857..8484)
                     /gene="gyrA"
                     /locus_tag="OO575_003153"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6666015.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            8631..9353
                     /locus_tag="OO575_003154"
     CDS             8631..9353
                     /locus_tag="OO575_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6666016.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(9481..12444)
                     /gene="yfaL"
                     /locus_tag="OO575_003155"
                     /pseudo
     CDS             complement(9481..12444)
                     /gene="yfaL"
                     /locus_tag="OO575_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2812 a         3490 c         3100 g         3042 t
ORIGIN      
        1 tccgttatgg cgtgagctgc ctcgctgaag cttacgtaac ccaccaccgg catccattcg
       61 ttcttcagac tcctgaagaa gcgttccatt gggctgttat cccagcagtt tccgcgccgg
      121 ctcatactct gtctgatctg gtatcgccac aataactgcc ggaactgcct gctcgtataa
      181 tgactgccct gatcgctgtg gaacatcacc ccgccgggct taccacgggt ttcccatgcc
      241 atttccagcg ctttcatggt gagcctgctg tccggcgaga acgacatggc ccagcccact
      301 ggttttcttg cgaacaggtc gagaacaacg gcgaggtacg cccagcgctt acccgtccag
      361 atataggtca catcaccgca ccacacctga tttggctcgg tcacggcgaa ctgcctttca
      421 aggtagttag ggatagcaac atgttcatga ccaccacgtt tataccggtg agtcggctgc
      481 tgacagctga ccagccccag ctctttcatg agcctgccag caagccagcg tcccatctgg
      541 tagcctctcc gggttgccat tgtggcgatg cttcttgctc cggccgaacc gtggctgatg
      601 ccatgtagct caagtacctg actgcgtaat acagcccgtc tgccgtctgg tttttcagga
      661 cggtttttcc agtatctgta gctgctgcga tgaaccccga acacatggca gagtgtgacc
      721 acaggataat gcgctctgag tttcccgatt atcgagaact gttcagggag tctgacatca
      781 agagcgcggt agcctttttt aatatttcat tctccatttc aatgcgttgt agctttttcc
      841 tgagctcacg gatttcaatt tgttccgggg taatggggga ggcttttggt gttttgccct
      901 gacgctcatc acgcagttgt ttgacccatc ttgtcattgt ggaaaggcca acatccatag
      961 ctttggcggc atctgccacc gtgtatttct ggtcaacaac cagttgagcg gattcgcgtt
     1021 taaactctgc gctaaaattt ctttttttca ttggagcacc tgtgttgttc tgaggtgagc
     1081 atatcacctc tgttcaggtg gccaaattca gtgtgccact tcatcattcg acattgacca
     1141 gttcaccagg ctggtaatgg gttttgtccg ttttcacccg gatatcaagc tgaggaacgg
     1201 caactttgat cccggcgttc tgaaaactgt actgaccatt acgggtatac agcaccgaaa
     1261 aagtgatgtt aggcgcaaag gaattgctca ctggtacccg ggcttcatac agggtatcgt
     1321 ttaaacgttg tagcgttagc cagtttgccg gatgcgaaag cagcgactgc tgttccacgc
     1381 gatcgcgttc cagcgtcaat aacgcttcat caattggctc cgggaaagta atcagcatct
     1441 tcgcggtttc gcctggctgg tacagcgttt tatccgccac gatatctacc gtaccagtat
     1501 gcgccgtgct gcccttaccg ctgacggcat gacttaaccc agcgagaatt aagccgtctt
     1561 tatcgcgtaa tgtcagattg tagttgccag gtttagcgaa attgacggta aaggatttgc
     1621 cgcctgacgg tagctctccg ctatggctcg tgcggtcttc gagacgcaac cattcatacg
     1681 taacaggaac ctgttttgaa gattccagcg cggcataacg gaacacaacc gactcgccgc
     1741 tattactgta ttgtgcggcg gtacttaaag agtaatgcgc caggccgcgt tcaatgagga
     1801 tctctttggt ggtggtgacg cgatacgccg cgccgtcact ggcggagacg gttaacaaat
     1861 agcggctcgg tttatcggcg gcggggagat ttaacgccac atgaccgctg gcgtcggaca
     1921 ccgtttcgct gccttccagc gacacgggga aacgtccggc ataacgcaaa tcgttaccga
     1981 ccattgataa ttgctgagcg cgcaaactta actgcacgcg ggcatttttt accggctcgc
     2041 catccgggta gagcagttgc agtttgccgc tgaccgcttc gccagttttg aactcttttt
     2101 tgtcgagagc taaaccaatc tcgaaatgtg gcttgatgta gtttgccacg cgaaaactgc
     2161 tgctatagac ctgattgcgg taagcaagac gtaactcata acctccggca acggcatttt
     2221 ctggcaggcg gaaacttccc tgcccgccat tgcgcgcatc cagcgtgaca tcgacggttt
     2281 gcaacagact gccgttagcg tccagcaccg aaagcttcgc cggggcgctg acgatggggg
     2341 atgaatgcaa cggatcgcgg aactcgcggc ccatcacttt aacatcgaca cgatcgcctg
     2401 cgcgatatag cgggcgatcg gtaaaaatat acaagcgggt gttgtagatt tcgctttcgt
     2461 agaagaagtt ctcggagaca aaaacgccgc cttcagcatc cttacccaga atgtatgaac
     2521 gttctggcga tatatgttgt aactgcaaag taccgctgtc atcggtcacc ccgcgggtca
     2581 tcacgccaag accttcagtc cacaagatct ctgagccggg cttcgcttca ccctgttttt
     2641 tacccgcggt ccataccaga agctcgttac ctgacacttt gctaagcgcc acggtatcgg
     2701 aaacaaacac caccgtcgtc gcccgatacc cacctaccat cgcctcgacg aggtacagcc
     2761 ccggctcttg ttggccgaga ggaatataaa tattacccgg ctgcggcgag atgaaattgc
     2821 tggatgcgcc ttccagtttt accccttgct gcggctcgac cggtttagcc tgccatagtg
     2881 gataacggaa ctgttccacc aagggatatt ttttcagcgg ggaaaactgg ttgttctgta
     2941 cataacggga aggtttaata atggcattgc cgagatgtaa ttcgggtaat gcctgagtca
     3001 cattctgccg tgactgagaa gagaaagtac gctgcatcac gcggcgagat ttgccgtacc
     3061 agttatccca cagccaggtc agcgtattgt tcagcccgtc gcccagatat tgcggttgca
     3121 ccacaatgcg atgcaggttt ttctgctggc gcaaaaatgc catcgggtca ggaatacgat
     3181 ataggcgaac gtccacgccg ccgtactctt ccatctgata gcgccgataa tcacgccccg
     3241 gcgcttccag tcgcactttc gcctcttcac tgctgctaaa actgctgtca gcaagcaaaa
     3301 agaatgttcc cccggcgggc ggcgcatagt tactggaagg aagcgaatca tcagctttag
     3361 caagccctgt tcccacaaga gacagggaca agcacgctgc tatcgcgcca gaaaatttaa
     3421 acgataaatg ccaatgaaat tgggattgga atcgttgggt atccatcggg tgtccttcca
     3481 tgtcataagt tgttgcagac tgactgcgcg cattccgttg tcagttttcg tggcgcttcc
     3541 ggtgtggtag atgacgtaac gccccatcca gaccattaag tgctgggcat cgccctgatc
     3601 gaaaaaaatc atatcgccag gcagcgcctg gtttatgtct tggccaataa actggctgtt
     3661 gtactgaatc aaattaatcg cggtcacgta ggggccgttt ttcccgctcc cctgattcca
     3721 gttttgcgcc agttgacgct gttcaggtgt tagcgtcatc tctggcggca aatactggct
     3781 agataaaccg ttacttttta gccatttact gtcgtgaact ttcagcgtct cgttcgccgc
     3841 aaatcgcacc aggcccgcac aatcctgctg ataccagcgt ggacttggcc cctggcggag
     3901 ctgttcttgt gcaatgcgta caaaccaggc gcgaaacagc ctggattgtt cgacattcag
     3961 catttcgcta tgggcaacaa cacaacacag ccagcaaatc agcgccagca gcccgtgtct
     4021 catagcggct gccaggttat gggtagccac tgccaggcga caccgggttc catctgggcc
     4081 agcttcatga cataacgcgg ttgttgagat aaagcgtcca gcttcggcat taataaagtt
     4141 tgtgcggcgt tataaaaaac cggttcgaga ttcttcggca gactggtcaa cgtttcgtta
     4201 cgcagcagtt tcgccacgcc ttgtggattg atatagagcg gaacgatgcc atcagtgggt
     4261 attacatcca ccattgccgg gcgggtttta ttcagtgttt gcagggcgtt attaaccagc
     4321 gtgtcatcga gggagaaaag cagcgttttg ttttgcatcg ccagcgacac acggaaaaaa
     4381 taatccgaca ttaattgatc gggttgcgcc gcctgcgctt tcggatactg accgtatcgg
     4441 gaactcactt cgcgacacca gatttgcgct tcgccctgct gagtctggct taccggcaaa
     4501 acaccttctg gcgctttgct ttcgtgcgca ccaatatttt gcgtaaacag tttccctggc
     4561 aattgcgcct gttcggcagt gccatcaaac tgaccgacaa acagcggggt ttgcaatttt
     4621 gagtcttcat accagcacag ccccgcggca ccgtctaacg ccccattcag cttgtcgttt
     4681 tcctggctga tgtgcgaaag catctcttcg gcaataccgt gtgaatacgg caccgccaca
     4741 cagaagctgg ctccggcagg catactgttc cagaccggcg taaaatcgaa actggcatct
     4801 acgctggcgg attcatcatt taacgccaca aaactgtgcc agccgtcgtt acccatttcg
     4861 aagcgtacgc cagcaaaaga aggcattaac cgctggtagc caaaacccag caacctggcg
     4921 ctgaccacga tgcgcaggcg tactggcgtt ttttcggctg tacgctcttc caggccaaag
     4981 ctggcttgcc agcgtttttt accgctcaac aaatcacttg cgatcgccgt ggcttcggta
     5041 tcctgctgat catctttaaa caacatatcc gtactggaaa acaccagcat cttgtcctga
     5101 tatgtcgcga acatcagggc gttattgccg ttatagcgca actgataaac aggaatagtt
     5161 tcactattta tcttgatgct actgatttcc gttttgctta actagctgtc gctggtagcg
     5221 gcaaacaaca atggctccag cagtttgctt aaaccactgc gctggatcag caccatgtaa
     5281 tgtgaaagat ggccttgttt atcgtgccat agcgccgcct gcgcgggctg atctaagagt
     5341 gacgaaaaga gcttatcttt cagcgtgaga tcgtgttcat agacaatgcg gcgaatgctg
     5401 ccttcaatgc ccagacgatc ggcatgattc tgataataga aaacgaaatc ttcgctcaga
     5461 acatcgtgga gaaacggaat ggtgaggaga tctttgggaa gctggctcag agagtcgctg
     5521 tctagaaaga ggtccggctc attgagatcg atttgcagat tgttgtgcac caccagcggc
     5581 gacaacgttt tttctggccc actgccagca tattgcaacg cccagacgcc agcggaaagc
     5641 agtgctattg cgccaaaacc tacaagacca tagaaccgcc agcctttcgc ctttttttca
     5701 ccactcattg ccacattcct tgtgtatagc cagccatttt ttacgggcac agccaaactt
     5761 taccgcgccc taatacgaca aaagcccaga ctttgcagcc tggacttttc aattcaaaca
     5821 agggagatag ctcccttttg gcatgaagaa gtaaaattat tcttcttctg gctcgtcgtc
     5881 aacgtccact tccggagcaa tttcatcatc cccttccgcg gcactgccgt cgatggtatc
     5941 cagatcttcc tcgtcaaccg gttcagcaac acgctgcaga cccactacgt tttcatcttc
     6001 cgcagtacgg atgaggatca cgccctgggt gttacggccc acgatgctga tttctgaaac
     6061 gcgagtacgt accagcgtac cggcatcggt gatcatcatg atctggtcgc agtcatctac
     6121 ctgcaccgcg ccaaccacta aaccgttacg ttcggtaacc ttgatggaga taaccccttt
     6181 cgtcgcacgc gacttggttg ggtattccgc cactgcagta cgtttaccgt aaccgttttg
     6241 cgttgcggtg aggattgcgc catcgccacg aggcacgatc agagagacga ctttatcgcc
     6301 ttcacctaag cgaataccgc gcacaccagt ggtattgcag cccatcgcac ggacagaaga
     6361 ctctttaaag cgcaccactt taccttcggc ggagaacagc attacttcat cttcgccact
     6421 ggtcagatca acgccgatca gctcatcgcc gtcaaccagt ttgatcgcca ctttaccggc
     6481 ggtacgcaga cggttgaatt cggtgaggac ggttttcttc acagtaccgt tagcggtcgc
     6541 catgaagact ttcacgcctt cttcaaactc ggtcaccggc aggatcgcgg tgatacgttc
     6601 gtcctgttcc agcggtagca ggttgacgat cggacgaccg cgcgcgccac gagtggcttc
     6661 cggcaactgg taaactttca tcgaatagac gcgaccacgg ctggagaagc acagaatatg
     6721 gtcgtgcgtg ttcgccacca gcagtcggtc gataaagtct tcttctttaa tacgtgcggc
     6781 agatttacct ttcccgccac gacgctgcgc ttcgtattca gaaagcggct gatatttaac
     6841 gtagccctgg tgagagagcg tcacgaccac atcttcctgg gtgatcagat cttccaggtt
     6901 gatgtctgcg ctgttggcgg tgatttcagt acgacgtttg tcaccgaact gttcacgaac
     6961 cagctccagc tcttcgcgga tcacttccat cagacgatcg gcgctaccaa gaatacgcaa
     7021 cagttccgcg atctgatcca gcagctcttt gtattcgtcg agcagttttt cgtgctcaag
     7081 gccggtcagt ttctgcaaac gcagatccag aatcgcctga gcttgctgtt cggtcaggta
     7141 gtacagacca tcacgcacgc cgaactctgg ctccagccat tccggacgcg cagcatcgtc
     7201 gccagcacgt tccaacatgg cggcaacgtt gcccagctgc cacggattag caaccagcgc
     7261 agttttcgct tctgcaggcg tcggcgcatg acggatcagt tcgatgatcg ggtcaatgtt
     7321 cgccagcgcc acggctaatg cttcaaggat atgagcacga tcgcgagctt tacgcagttc
     7381 gaaaatagta cgacgggtca ccacttcacg gcggtgacga acaaacgccg cgatgatgtc
     7441 tttcaggttc atgatcttcg gctgaccatg gtgcaatgcc accatgttga taccgaaaga
     7501 aacctgcaac tgggtctggg agtagaggtt gttgagcaca acttcaccga ccgcatcgcg
     7561 tttcacttca atcacgatgc gcataccgtc tttgtcagac tcgtcacgca gcgcgctgat
     7621 gccttccacg cgtttttctt ttaccagttc cgcaatcttc tcgatcaggc gcgctttgtt
     7681 tacctgatac ggaatttcgt ggacgataat ggtttcacgt ccggttttgg cgtcaacttc
     7741 cacttcagca cgggcgcgga tatatacctt gccgcgaccg gtacggtaag cttcttcaat
     7801 accgcgacga ccgttaatga ttgccgccgt cgggaagtcc ggccccggga tgtgttccat
     7861 cagcccttca atgctgatgt cttcatcatc gatatacgcc agacaaccgt tgatgacttc
     7921 cgtcaggttg tgcggcggga tgttggttgc catacctacg gcgataccgg aagaaccgtt
     7981 caccagcagg ttaggaattt tggttggcat gacgtcggga attttttccg tgccgtcata
     8041 gttatcaacg aaatcgaccg tctctttttc gagatcggcc atcagttcat gggcaatttt
     8101 cgccagacgg atttccgtat aacgcattgc cgccgcagag tcgccgtcga tggaaccgaa
     8161 gttaccctga ccgtctacca gcatgtaacg cagcgagaat ggctgcgcca tacggacgat
     8221 cgtgccataa accgccgagt caccatgggg atggtattta ccgattacgt caccaacgac
     8281 acgggcagat tttttatagg ctttgttcca gtcattgcct agtacgttca tggcgtaaag
     8341 tacgcgacgg tgtaccggct tcaggccatc tcggacatct ggcagcgcac ggccaacaat
     8401 gaccgacatc gcataatcca gataggagct ctttagctct tcctcaatgt tgaccggtgt
     8461 aatttctctc gcaaggtcgc tcatctaacc gctatccctc tactgtatcc cggattcaaa
     8521 ggtcgcaaat tataacacag ccgcgcagtt tgaggtaaac ctatacgctt tattcacatc
     8581 caatgcctga tatactcgtt tgtcttgcca attacggagt agaagtgcca atgaatgccg
     8641 aaaaatcgcc ggaaaaccat aacgtagacc acgaagagat cgctaaattt gaagccgtcg
     8701 cctcccgctg gtgggatctt gaaggtgagt tcaaaccgtt gcaccgcatt aacccgctgc
     8761 gtctgggcta tattgccgag cgtgctggcg gtttatttgg caaaaaggtg ctcgatgtcg
     8821 gttgtggcgg cggcattctg gccgagagta tggcgcgcga aggcgcgacg gtgaccggtc
     8881 tggatatggg ctttgagcca ttgcaggtgg caaagctgca cgcgctggaa agcggcattc
     8941 aggtggatta cgtgcaggaa accgtggaag agcacgcggc aaaacatgcc gggcagtatg
     9001 atgtggtgac ctgcatggag atgctggagc acgtacccga tccgcagtca gtggtcagag
     9061 cctgtgcgca actggtgaaa ccaggcggcg atgtcttttt ctcgacgctt aaccgcaacg
     9121 gcaagtcatg gctgatggcg gtggttggcg cggaatatat tttgcgcatg gtgcccaaag
     9181 gtacgcatga tgtgaagaag tttattaaac cggcagagct gcttggctgg gtggatcaga
     9241 ccagtttaaa agagcgacat ataaccgggc tacattacaa cccgatcact aattccttta
     9301 agctcggccc cggcgtggat gtgaactata tgctgcacac gcagaataag tgaggttgat
     9361 gtttggccgc gccaatgcct gatgcgacgc ttgcagcgtc ttatcaggcc tacaaatgct
     9421 ctccgtaggc cagataaggc gtttacgccg catccggcaa ttgtgatggt cagacagcga
     9481 ttaccatttc accgtcatcg acaaaaaccc tgccgtctgg gcaaaatcat cactcccttt
     9541 ctgccacgcc acgctgccgc gcagggacac tcgctgactg atattgcccg tgactcccac
     9601 ttttatttca ccccgtagct tcaccgcatc gtcactgata gtgctgccat cttcttcaat
     9661 ttccgtcgca tggggatcgt gatagtaatt cagatctaat gttggtgtga caccaaccgc
     9721 ggtacgccat tcgctgtgta aacccagccg cgtctgaata tcatccccct gcgattgtga
     9781 cacgcgcgca tggttagcgg cggtaaaatc atcctgctgc acgccctgat aaatcacctg
     9841 cgcctgcggt tcaatcacca caccacgccc cggtaaccac tgatacccag cttccagcga
     9901 ggcgataatc cccgacgaat ggtaacgatc cacgccatct tcatgttcgg aaacatcatt
     9961 gttaaaccac gcgtattgca gccagctatc cagccaggcc ccctgcttct gattaccgtg
    10021 ctgataccag cttgaggtca gcccaacggc ataaccgtgg ttctggttat cggcgcgagt
    10081 tccggtcata ttcgagcggc tgtcgccctg attatcactg tagccaccaa ccgccccaag
    10141 catccactcg ccatccgtgc cccagcgccc gctaaacaag ccgccgctaa gctgcaccgt
    10201 agaagtgtct tcctgctgag ccagttgccc tgccgccgtg taatgataac gtccgccgat
    10261 aacacgtaaa ttcagcgtct gaccatcgcc accagcgtga tcgcgtcgct ccatcataaa
    10321 cgcctgattt gccgcccgca ggttattgag ataaccgcca actttggcat tcagcaccgg
    10381 ctggtaagca ggcgtagggg ccgggtcggg ggttgggtca ggatccggcg tgggatcagg
    10441 atcgggagtc gggtctggct caggtggcga tggcggcgtt acttcttgcg atcgcagata
    10501 ccagtcgttg ttatcttcca ctagcgtgta gtcatacgct cccatgttga cgtagccact
    10561 gccagccaga ctgaactgtg cattgttttg aaactgcgtg ggatcagctg cgaaatcaac
    10621 cactttaatg cctgtcgatg tcggctcacc aatccctgta atggagttaa ccaccacagt
    10681 tgtgttgcca gcagtattac cgttcatcac caattgatcg cttaccgagt catcgccgtt
    10741 taattcgctg tcgaatagta gcgtaccgcc gccggtataa tcaccgttta ccgtcagcgt
    10801 atcgccagcg acgccatttt gcaggctaac cgcaccggac gtattggtca gatcaccatt
    10861 aaccgtcacg tcagcaagat aggtcaggct gtcagtttca aggttggtat taagctcgcc
    10921 tgtcaacgtc acgccatcgg tagcatttac cagcgtccca tcgccgttaa atagtgaggc
    10981 attaagggcg acggaagtat cctgcccggt cagacgcaaa accgtaccat cgctgatgtc
    11041 gatagtgccg ctggaagtag catcaattga ctgaatatcc tgatccgcgc cagtaacgaa
    11101 cgttgcccca tcaccaaccc acagtgacga agcataagga aagatatccg caacatcgcc
    11161 tttcagggtg ccttcttcga cacgtaccgc cgactgcgtt gtaccgctgg cggtcagctc
    11221 cagcgtaccc gcccccgttt tagtaaatgt gctaccctca tcctgatgct gcccgctgct
    11281 gtcagccatc agtgcgcccc actgcgtgtc tacccccgca tcaactgcca cttcaccgtc
    11341 ggcgcgcatt tcaatatcac gcccatgctc agtcgtcgta gagcgagtat cgacattggc
    11401 agtaatctcc atcacgcttt cttgttactt atcggtaaag ataacctggc ggttatagtg
    11461 ggtatctcca agctgcgagt tgtcgctcac catcagcgta ccggaggcga tttgtgttgt
    11521 gcccaaataa ctattattgt ttgccagact tacctgcccg tcgctcgcat cgaccaccac
    11581 gtagacgcca tctttcccgt cgccgccgat atgcaaattg tcgccacctg caagatctac
    11641 cacatcctga ctgccgataa ccgttccgct gctgccactg acttcaaggc catcattgta
    11701 tgatgtgccg ctctgccaag tggagaaatc agagagatcg agcacaccgc cgtttaacac
    11761 gatcgactgc ggatcgtcct ggagagcggt aagatctgcc gcgtcgcctt ccagcgaaag
    11821 caccgcacca tcatcaacga ctatatcgcc ggttagcgcc atcgactgcg cccctgccag
    11881 cacgtagctg ccgttttgcg caatggtgag ctgaccagca ccttcgatga tgccagcaaa
    11941 actgccctga ttaacagtaa cgttgccacc agcatcgata tttaaagtgc cattctgaaa
    12001 gcccgtcaat gcgtgcacaa aagtttgttg ggtcgagcca acgtttagct cagcctgatt
    12061 ctgatactga tcaatactcc ctatcgtcag accgtagcag tcttgcggat cgtcctggca
    12121 atgcgtatcg ccgacattca tcagggagtt gctgcgaccc agggtaactt caccgttttc
    12181 aatctgcatc tcaccagtaa agtcattgtt atctgcatta agtaccagat cgccggaacc
    12241 tgttttggtg attaacccgg taccagcaat agagtcaaca gctccgtcat tctctgtatt
    12301 gccgataacc agcgtttttc cgtcggcaat atcaaaggta acttcactta agccgagata
    12361 cataaagcca cccgccgcag tggagggacc atctccatag cctgctgcgc tattgttctc
    12421 atcgactaac acgccgccgt tctg
//
LOCUS       DAJTYM010000101        12439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTYM010000101 DAJTYM010000000
VERSION     DAJTYM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..100
                     /gene="torD"
                     /locus_tag="OO575_003156"
     CDS             <1..100
                     /gene="torD"
                     /locus_tag="OO575_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6666017.1"
                     /translation="FAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(193..498)
                     /gene="cbpM"
                     /locus_tag="OO575_003157"
     CDS             complement(193..498)
                     /gene="cbpM"
                     /locus_tag="OO575_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6666018.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(498..1418)
                     /gene="cbpA"
                     /locus_tag="OO575_003158"
     CDS             complement(498..1418)
                     /gene="cbpA"
                     /locus_tag="OO575_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6666019.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1679..2935
                     /locus_tag="OO575_003159"
     CDS             1679..2935
                     /locus_tag="OO575_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6666020.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            3228..4469
                     /gene="agp"
                     /locus_tag="OO575_003160"
     CDS             3228..4469
                     /gene="agp"
                     /locus_tag="OO575_003160"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6666021.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4507..4734)
                     /locus_tag="OO575_003161"
     CDS             complement(4507..4734)
                     /locus_tag="OO575_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6666022.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(4755..5351)
                     /gene="wrbA"
                     /locus_tag="OO575_003162"
     CDS             complement(4755..5351)
                     /gene="wrbA"
                     /locus_tag="OO575_003162"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6666023.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            5724..5831
                     /locus_tag="OO575_003163"
     CDS             5724..5831
                     /locus_tag="OO575_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666024.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            complement(5913..7241)
                     /gene="rutG"
                     /locus_tag="OO575_003164"
     CDS             complement(5913..7241)
                     /gene="rutG"
                     /locus_tag="OO575_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6666025.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGMMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(7262..7756)
                     /gene="rutF"
                     /locus_tag="OO575_003165"
     CDS             complement(7262..7756)
                     /gene="rutF"
                     /locus_tag="OO575_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6666026.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(7767..8357)
                     /locus_tag="OO575_003166"
     CDS             complement(7767..8357)
                     /locus_tag="OO575_003166"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6666027.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(8367..9167)
                     /gene="rutD"
                     /locus_tag="OO575_003167"
     CDS             complement(8367..9167)
                     /gene="rutD"
                     /locus_tag="OO575_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6666028.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(9175..9561)
                     /gene="rutC"
                     /locus_tag="OO575_003168"
     CDS             complement(9175..9561)
                     /gene="rutC"
                     /locus_tag="OO575_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6666029.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(9573..10265)
                     /gene="rutB"
                     /locus_tag="OO575_003169"
     CDS             complement(9573..10265)
                     /gene="rutB"
                     /locus_tag="OO575_003169"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6666030.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(10265..11356)
                     /gene="rutA"
                     /locus_tag="OO575_003170"
     CDS             complement(10265..11356)
                     /gene="rutA"
                     /locus_tag="OO575_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6666031.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            11644..12282
                     /gene="rutR"
                     /locus_tag="OO575_003171"
     CDS             11644..12282
                     /gene="rutR"
                     /locus_tag="OO575_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6666032.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         3176 a         3135 c         3156 g         2972 t
ORIGIN      
        1 gtttgctgcg cgttgtcgac agtatgacag ctttggtttt tacgcggcac taagccagtt
       61 attgctggtg ttagtggagt gcgaccacca aaacagataa cgtcgtttat gcgcctgaaa
      121 agacgcgttt agcgtcgcat caggcattat ggcgcagttg ccggatgcgt catcacatcc
      181 ggcaaacaca catcacggat gagctacaaa ccgggaaagc cgctggcgta gcaggcggtt
      241 ttcctgcttc aggtgcgcaa tatcatccat taacgtcagc gccaccgcga tccccggcca
      301 gtccagagcc agttcatgac gcaggcgtac cgcgcgttgc accacaatgg cggcatggtc
      361 gtcaaatacc caggttgttt cctggatctc acgcggttca accaccccca aaccgacaat
      421 ttcattcaac tcctcttcag agatgccggt atgcaggcaa aattcggtaa tagtaaaagt
      481 caccgtaaca ttagccatta tgctttcccc caatctttac gtggatcaaa agacgactgg
      541 gcgtctgcca gttgctgcca cagcgcggca gtgttttcat ccggtttcgg cggcatcacg
      601 attttcagta ccgcatacag atcgccggtc tgttttttgc tcaccagacc tttgccttta
      661 acgcgcaatc gttgcccggc ctggctgcct ggcgggatag tcagcaaaat gctttctttc
      721 agtgttggaa cggtgacttt agtacccagc gccgcttccc acgggctaac cggcaccaca
      781 atttccagat cctggccgac aatatcgaac agcggatgtg gcgcaatatg aatcaccagc
      841 cacaaatcgc catttggacc gccgttttcg cccggcgtcc cctggccttt caggcggatg
      901 cgttgaccat tgccgacgcc tgccgggatc ttcacattca gcgttttcgg gatttcctgt
      961 tcgatcatgc caaaggcgtt ataaaccggc aggttatagc tgatggtacg cttatgctca
     1021 gtaagcgttt cttcgaggaa taccgccact tcgatttcaa tatcgtggcc acgtgcggcg
     1081 gggcgttgac ggctctggcg ggcatgctga ccgaaaattg acgagaagat atcgtcaaaa
     1141 tcttcggcat taaaactctg accgtcgcca tgatggaact gacggttaaa ttgcggatcg
     1201 ttgcgatgtt gccacatctg atcatactca gcgcgacgtt gttcatcact taacacttcc
     1261 caggcttcag cgacctcttt gaagcgggct tcggcatccg gttctttgct gacatcagga
     1321 tggtatttac gggccagtcg acgataggcg gtcttgattg tcttgagatc gtccgtcggt
     1381 ttcacgccca tgatggcgta ataatccttt aattccatag cgttatctcg cgtaaatcaa
     1441 cacaaattga aggaacccct gtaaggtaac tcctataagt gtagggtaat cctcaaaatt
     1501 tcatatgcca acacagaata tgttattgaa atcatcgcgg agaggaggtc gccatcaaga
     1561 tgggttgctg aacatatttt aaacaggtga aaaagggtga gcgatttttg atagttgaac
     1621 caggcacttt aagtttaact agggcgtcat tatttattaa attttataga cgctatatat
     1681 gggtagtaat atacatggaa ttagttgcac tgcaaataat tatttgaaac aggcctggaa
     1741 cgatataaaa aatgagtacg aaaaaaatca aacatattca atcacgcttt ttgaaaacac
     1801 actggtgtgt tttatgcggt tgtacaatga actcagacgt aaagtaaatg aagaggatac
     1861 tccatgtctg gaatgtgaat cactagaaaa agaatttgag gaaatgcaga atgataatga
     1921 tctatcatta tttatgagaa cattgcgtac taatgataca caaatttatt caggggtttc
     1981 aggaggtatt acatatacta tacaatatgt tcaagatgtt gatattgtta gagtgtcctt
     2041 gccgggcaga ggttcagagt ctatcacaga ttttaaaggt tattattggt atggctttat
     2101 ggaatatatt gaaaacatta atgcgtgtga tgatgttttt tctgagtatt gtttagataa
     2161 tgaaaatatg agtatccagc cagaacagat aaatatgccg ggaatatctg atttagatac
     2221 tggtattgat ttgtctggca tatcttttat tcagagtgaa attaataaaa catatggatt
     2281 aaaatacgct ccagtagatg gcgatggata ttgtctgtta agagccatac tggttttaaa
     2341 agaacatgag tattcatggg cgctgggcag ccataagacg caaaagcaag tatacgaaga
     2401 gtttattaaa attgttgata aacaaacgat cgaggctctt gttgatacgg cattctataa
     2461 tctcagggaa gatgtaaaga cattatttgg cgttgatctg caatctgaca acaaaattca
     2521 gggtgtgggt agttttatgt catggagctt tctgtttttt aaaaaacaat tcattgatag
     2581 ttgcttgaat gacaaaaaat gcatactgca tttacccgag tttatattta atgataacaa
     2641 gaatttgctt gctttagata ccgacacgtc ggataggatt aaagcggtga agaattttct
     2701 tgctgttctt tcagatagca tttgctcatt atttattgtt aatagtaatg tggcatcaat
     2761 ctccttggga aatgaatcct tttcaacaga tgaagatctt gagtatggat atttaacaaa
     2821 cactggcaat cattatgatg tttacctccc tcctgaactt ttttctcagg cttataagtt
     2881 aaaaaataag gaaatgaatg cgcaacttga ctatttaaat cgttatgcaa cttaatggca
     2941 aaggcatatg ctaaaaacca ttgttattag tctcacactt ttttattggt aaatattgtc
     3001 tctgtattgg taacgccgca gatattctgt ttagccacag gtgcaattat cagcggcgta
     3061 cgcgaggcag gggctaatca ggcatagttt gcgtcaaacc ttgcctgttt ttgaagatgt
     3121 atatagaaaa acaggcgttc aacaagccat tttgcgaacc tgttcccgga aaaaagtcat
     3181 atttctgtca cactctttaa tgattgataa caaaagaggt gccaggaatg aacaaaacgc
     3241 taatcgccgc aactgtggca gggatagttt tactcgcttc aaatgctcag gcacaaaccg
     3301 taccggaagg ctatcagcta cagcaagtgc tcatgatgag ccgccataac ttacgtgcgc
     3361 cgctggcgaa caatggcagt gtgctggagc agtcgacgcc gaataaatgg cccgaatggg
     3421 acgtccccgg tgggcaactc accaccaaag gtggcgtgct cgaagtgtat atgggccatt
     3481 acatgcgtga atggctggca gagcagggga tagtgaaatc gggggaatgc ccgccgccgg
     3541 acaccgttta tgcctatgcc aatagtctgc aacgtaccgt cgctaccgcc cagttcttta
     3601 ttactggtgc attccctggg tgtgatattc ctgtgcatca ccaggaaaaa atgggcacta
     3661 tggacccaac ctttaacccg atgatcaccg atgattccgc cgcattcagt gaacaggcgg
     3721 tggcggcaat ggagaaagag ctcagcaaac tccagcttac cgacagctac cagctactgg
     3781 aaaaaatcgt taactataaa gattcccctg cctgtaaaga gaaacaacag tgttcgctgg
     3841 tggatggcaa aaataccttt agcgccaagt atcaacaaga accaggtgtt tccgggccgc
     3901 tgaaagtcgg caactcgctg gtagatgcgt ttactttgca atactacgaa ggttttccga
     3961 tggatcaggt ggcctgggga gaaatcaaat ctgaccagca gtggaaggta ttgtcgaagc
     4021 tgaaaaacgg ttaccaggac agtctgttta cctcaccgga agtggcgcgc aatgttgcga
     4081 aaccgctggt cagttatatc gacaaagccc tggtcaccga tcgcgccagt gctccgaaaa
     4141 tcacggtgtt agttgggcac gactccaaca tagcctctct gttaacggcg ctggatttca
     4201 aaccgtatca gttgcatgac cagaacgaac gcacgccgat tggcggcaaa atcgttttcc
     4261 agcgttggca tgacagcaaa gccaatcgcg atttgatgaa aattgaatat gtgtaccaga
     4321 gtgcggaaca gttacgtaat gccgatgcgt taaccctgca ggcacctgcg cagcgcgtga
     4381 cgctggaatt aagcggttgc ccgatagatg ccaacggttt ctgcccgatg gataagtttg
     4441 atagcgtgtt gaatgaggcg gtgaaataac agaaaactcc cccgcgagaa gcgggggagt
     4501 cgctggttaa acgtttttac gttcgatggt ctgttcgccc caaaaaagcg aatctttatc
     4561 ggtcttagca aaggctttga ctaacacttc atcactacct tcttcccaaa tcttttctgc
     4621 cattttttcg tcgtacccgg cgacttcgaa aatggcctcg gctatttccg gcgacgtatt
     4681 gcgcagagat gcccattcac cgacgtgatg agctttcgct tcttgagttg gcatgcgtat
     4741 cctcctgttg aagattagcc gttaagttta actgccagac ctgcgacata ttccccttga
     4801 taacgagcaa tagacagttc ttcctggctt ggctggcgtg agccgtcacc gcctgcgatg
     4861 gtggttgcgc cgtacggcgt accgccgcga acctgtgaaa cgtcaaataa ttcctgcgct
     4921 gcgtagccaa tggggacaat caccatgccg tgatgcgcaa gggtcgtcca ggtggatgtg
     4981 atggtttgtt cctgaccgcc gccagtaccg gtggaactaa agacgctcgc cagttttccg
     5041 tatagtgcgc cggaagccca caggccgccc gtctggtcga ggaaggtacg catttgaccg
     5101 gacatgttgc caaagcgggt aggtgtacca aaaataatgg cgtcgtaatc ggccagttct
     5161 tgcggggttg caaccggtgc agtttgcgtt ttaccgcctg ctttttcaaa taattgcggc
     5221 ggcatggttt ccggtacacg cttaacgaca acttctgcgc catccacttt gcttgcaccc
     5281 tcagcgactg cgcgtgccat cgtttcaata tgtccgtaca tggaataata aagcaccaga
     5341 actttagcca tttctaacca ctcctcgtgt tatctctatt ccgtagcgat tcgctaccac
     5401 ttatttaaag ataagacgtc cttttcagag tgcaaatttc acaaccactt atttgattta
     5461 taacaacttt cacaagcacg taattttgtc gcaaaatgac acatttttat ctcatcgcgt
     5521 ttttttaatc ataagagcgg cttatggata attattggag atgatatcta ttctcgctaa
     5581 gaagctgttg caggatatta ccaaacgcgg gtctgtccgc gtcagttcac taagcttagt
     5641 cccacgtagc gaaaatatgg cagccgccat acgccgcgtt aattctatgc aatatgatgt
     5701 ctatacccag acggaggtca gtaatggcaa accatcgagg cggttccggc aattttgcag
     5761 aagaccgcga aagagcatca gaagcaggta aaaaaggtgg caagagcagt cacggcaaaa
     5821 gcgacaacta gccgggctaa tcaatgacga atgcattttt ttcttaagct cgtcaaaaag
     5881 tcatcaccgc cggtaacccg gcggttttct gtttatggct cctgatgaac aacttctggc
     5941 ggtggaacgt caaccaacct tctgcttaat aacgcattga gtaaaatcgc gccaaaggtt
     6001 gctgtaccga tccctcccaa cgtaaaaccg ccaagcgtga gagcaaaatc acccgcgccc
     6061 agcacgaagg ttactgcgac cataatcaaa ttagcgttct ggcttaaatc gacacggttt
     6121 tgtacccata tccttgcgcc tgcgacggca atcagcccga acacaacaat tgatgcacca
     6181 ccaatcaccg cggccggaat ggtatggatc agcgcgccaa atttcggcga aaagcccaac
     6241 agcatagcga tgacggcagc tgcaacaaac accagcgtcg agtagacttt tgtcacggcc
     6301 atcacaccga tattttcagc ataggtggtg acgccgctac cgccgacaga gccggaaagc
     6361 atcgttgcca gaccatcacc gacgaatgct cgccccatat acgggtccat attgcgtccg
     6421 gtcatcccgg cgaccgcctt gagatgacct aagttttccg ccaccagaat caccgccacg
     6481 ggcgcaatca acatcatcgc ctggccatta aaaccaggag tggaaaaatg cggcagaccg
     6541 aaccaggcag catagctgac gagagtaaaa tcgacggctt ttcccagccc taaaacgttg
     6601 gtcatcatgc catacagcag acaggcgaca attaatccta cgagaatcaa taaccgctgg
     6661 atcatgccac gggtaaacac cgccaccagc ccaatacaca gcaccgtcat taccgccatc
     6721 cagctatcaa aggccgaagc cgatacactt ttcactgcga taggcgctaa gttcaggcca
     6781 atcgccatca ccaccgcacc cgtcaccacc ggcggcatta gtcgttcaat ccagcgtgta
     6841 ccaattttca tcaccaccag gccaatgacg gtataaacca gcccacaggc gataatcccg
     6901 cccagcgcga tgctgatatt cgggttaatg ccctgaccgt taaagcccgt cgcggcaatc
     6961 accacgccga caaaagccgc gctggagccg agataactgg ggacgcgccc gccggtaata
     7021 aagaaaaaca gtaacgtgcc gatccccgac attaaaatgg aaagattggg atccagcccc
     7081 atcagaatcg gcattaacac cgtcgcgcca aacatcgcca ccgcgtgttg aacgcccatt
     7141 actgccgtct gagcaaacgg caatcgttca tccggcgcga ccacgccgct ctctgtagag
     7201 gtcgatttta actgccagtg aggaaaaccg aacattgcca tcagctgtct ccttaaggag
     7261 gttaacaagc agggcgcatc agcgcgtgat aactgcgatc gaaccacacc agcccgtagg
     7321 gtgtggtgtg acgatgaatc gcttcgatgg cgcaaaacag aatgtcgtgg gtgccgacgc
     7381 tcaccacctg gctgatacgg cagtcaaacg aaaccagagc ctcttccagt tgcgggcatc
     7441 cggtcacacc cgtctgccag cgggcggcgg caaagcggtg ttccatgggc gttttgccgc
     7501 caaaaagatt tgaaagcggc tcctgcccgg cgctcagtgt atttacacac agcgttcgat
     7561 tttcattgaa taccggccag acggacgccc cacgattcag gcacaccagt aatgtgggcg
     7621 gcgtgtcggt cacgctgcag acggcgctgg cggtgaaccc ggcgcgcccg gctggaccgt
     7681 ccgtggtgat gatattgacc gccgcgccca tacaggacat cgcatcgcga aaggtttgtt
     7741 gatcaactat gttcatagtt tgctccttac aacagcccgc aggcttcttc aaaggacaga
     7801 cgtggcaggc gcgcaaaaag tttgctgcta tcgccatagc cgatattaat cagcagattg
     7861 ctcttcagcg tgctgcccgc aaaaaaggcg tcgtcaacgt gttgacggtc aaaacccgac
     7921 atcgggccgg tatccagtcc cagcgcacgg caagcgacga tcagataagc cgcctgcatg
     7981 gaactgttgc gaaacgctgt ttcttcggca agttgtgggc tggaggtaaa ccaactgcgg
     8041 gcatcaccgt ggggaaacag tagtggtaac cgttcataaa attcactgtc ccaggcgacg
     8101 atagcggtga cgggcgcggt cagggttttt tgcagattgc cgctggaaag cgccgggcgc
     8161 agacgttctt ttccttctgc agtgcggata aacacgatcc gtgccggaga acagttagct
     8221 gatgtcggcc cccatttcat cagggcataa agctcctgta acgtctcatc gctgacgggt
     8281 gtctcccgcc agccgttgtg agtgcgggca tcggtgaaca gggtgctaag cgcacctggg
     8341 ctaacggctt cgttcatagc aattccttac agggcggctt cacggtgatg taacaggctg
     8401 gcaagcccgt tgagtaacag agcattaaac gtttcgggat cggtcacgtt gcaggcgtgt
     8461 ccgccatagg gcatcaccat tttctggcta tcgggcaggg cggcatgaag ttcactggaa
     8521 catgctgttg gcaccagcag atcatcactg gcgcagatga tttgcaccgg gcagcggatg
     8581 cgatccgcat ggtgactaaa gtcagcgcgt ttgagggcgt taagtcgacg tagtaaatta
     8641 tttttgccct gaaaatgcgc cagtgccagc gcgtcttctg cctccaggcg aggtgcgcgg
     8701 gccgccatcc agtcggcggg atagaggaac aacggctgcg cttccaccca tgcttgcgcg
     8761 ccgccgctat acagtaatcg ttcgcgaacc tgaaaacagc ggcgcgtatg ggcgtttatt
     8821 cgtagccagc cgttaacgct gaccagcata gttaccgacg cgggataatc cagcgccagc
     8881 tgcatcccca ccagcgcacc gagcgcatgg ccgaccaccg cgtaacgctc aatccctgcg
     8941 gctaccagcg cctgatgcag ttccgctgcc atctgggcga tactgtaatc ttctgccagt
     9001 gtgtcgggat tattgccggt gccgcgctgg tcgtaacaga ctacctgata ctcctgctcc
     9061 agcaccgcca gttgcggtaa ccagtaactg ccgctaccgc caagacccga aatcaacacc
     9121 actacgggtg catcagcata agggggagat gagagtgaaa gtttcatcgc ggcctcactt
     9181 ggcgatatgc gcaattgtgg cgatttccac cagcgcgtca ggttttacca gtccgcactg
     9241 aatgtagaat cgcgccggtt tatcacccgg aaaaaactcg gcgtagattt cgttaatcgc
     9301 ggcgtaattt ttccagtcgg taataaagat gctgttgaag gtcacatccg ccatcgtgcc
     9361 acccgccgtc tcgatcacct tgcggatagt ttccagaacg tggcgggttt gcgcctttgg
     9421 gtcatcggca aacagcacgt tattatgttg atcaaaagcc agcgtaccgg agacatacac
     9481 cacgccatca gccagcgtgc cgggaacgaa gggggccagc ggtgcgctgc tgccagcggg
     9541 aataattacg gattttggca tcgctaaact ccttaagcga tacgagcaaa ggacgtggga
     9601 gaaagcgcgt cgcagaacgt ttcgacgtcg ctgacccagc caaaaaaggt ttcgatattg
     9661 aacaacgcgg ctttctgtgc aaattccggc cccgcctggt gtgttgcgtc ttccagcacc
     9721 acgccgaaat gctccagaaa aaagccatcg cgtagcgtcg attcgacgca gacgttggta
     9781 gcgataccgg tgaaaaccag atggcgtatt ccacggctgc gtaaaatgct gtccagcggc
     9841 gtattgaaga aaccgctgta gcgcggcttc ggcagcacaa tatcgccagg ctgcggcacc
     9901 agttcatcca ccagttgata atcccaggag cctttcgcca gcaatttccc ctgcagctgc
     9961 ggctgcttac gcatggtttt cagggcgttc gatttatgaa aattcggtga gccggggccg
    10021 ccagcctcga catactgttc atcccagcca ttttgaaacc agatgatcaa catccctgcc
    10081 gctcgcgctg cggtcacagc ggtttgaatg ttggcaatga ccgggcgagt ggttgagaca
    10141 tcaaacccgg cgagatctaa gtagccgcct ggcgtggcat aagcgttttg catatccacc
    10201 acgatctgcg cactttgctg cggatcgaag gtaatggctt ccggtcgagc ggttaaggtt
    10261 gtcatcatgc cacctcctga gtcagcgcag agagatgggc gcggcactgc atcagtggtt
    10321 gaatgcgctc gccgaaggtt tcgattcccg acagaaaatc atcgaaggtc aacagcacgc
    10381 cttctgcacc aggcacgctt gcgacttcat ctaacatgcg cgcgacactg gcgtaagaac
    10441 cgactaacgt ccccatattg atgtttaccg ccgaagtggg atcggccatc tgacgaacgt
    10501 tggtgtcagt accggagcgg gtatctttct gactttgttc ggttagccag cttaacgcct
    10561 cttcatccgc gcccgctttg tagtgttccc atttggcgcg agtggcatcg tcggtttcat
    10621 cggcaatcac cataaacaac acataagagc caacgtcgcg tccggtttgc tctgtggcct
    10681 gtttcatccg cgcagcggtc ggggcgaaag ccgtgggtgt atttacgcct ttgccgaaac
    10741 agaagttgaa atcggcatac tgggcggaga acgccatgcc agcgtcgctt tgcccggcgc
    10801 agatcacttt catggggata ctcggttgcg gactgacgcg acaatcattc atggtgaaaa
    10861 aatcgccttt aaaatcgctt ttccccgtgc cccacaggtc gcgcagcacc tgaacatatt
    10921 cggtgagata gtcgtaacga cgggagaaat agtcatcgcc aggccagata cccatctgct
    10981 catactcggg cttttgccag ccagtcacga ggttgacgcc aaaacgcccg ccagagatgg
    11041 agtcgatggt tgcggccata cgggcgacga ttgccggagg taacgttaag gtggcagcag
    11101 tggcgtaaat ctgaatgcgc gaggtcacgg ctgccagacc cgccatcaag gtgaacgact
    11161 caaggttgtg atcccagaac tcagttttgc cgccaaagcc acgcagtttg atcatcgaca
    11221 gggcgaaatc gaaatggtaa tgctccgctt tttgcgcaat ggctttattc agttcaaagg
    11281 tcggcatgta ctgcggcgcg tgggtcgaaa tgagccagcc gttgttgcca ataggtacga
    11341 atacgccaat tttcatcatc aacctctctt cgtctcgtaa agtgaaagtc agacggggcg
    11401 ctgcatcctg catatccttt tcagccgcgt attggcttgt tcgcaaagcg gatgccagtt
    11461 tttaaaaagt gaatattatt aatgtgttag cattgtgtta tctaaaatat ctggtaaaaa
    11521 gtggactaaa cggtcaaaac agttgcacat aaaacatgca tccgtgcgcg atgagagtgc
    11581 agaaggtcga ggccgggcgg gggttttgct atcctgttac caatctacaa gaggggagag
    11641 cgcatgacgc aaggcgcagt gaaaacaacg ggtaaacgtt cgcgcgcagt aagcgcgaag
    11701 aaaaaagcga ttcttagcgc agcactggac actttttcac aattcggttt tcacggcaca
    11761 aggctggagc agatcgcaga gttggcgggt gtttcaaaaa ccaatctgct gtattacttc
    11821 ccgtcaaaag aggcgctgta tattgccgtg ctgcggcaga ttctcgatat ctggctggca
    11881 ccgttaaaag cgtttcgtga agatttcgcc ccgctggcgg cgataaaaga gtacatccgt
    11941 ctgaagctgg aagtctcacg cgattatccg caggcttcgc gcctgttctg tatggagatg
    12001 ctggcaggcg cgccgttgtt aatggatgaa ctgacgggcg atttgaaggc attaattgat
    12061 gagaaatcgg cgctgattgc cggttgggtc aaaagcggca aactcgcgcc gattgatccg
    12121 cagcatttga tttttatgat ttgggcttcc actcaacatt acgccgattt cgcccctcag
    12181 gtggaggcgg tgacaggcgc gacgttgcgc gatgaggtat ttttcaatca aacggttgaa
    12241 aacgtgcagc ggattattat tgaggggatt cgaccacgtt aaagatgacg gaggaggttg
    12301 taacatcctc cggctacctg tttaaccgat cgtcattaaa ctggcgttac cgccagcggc
    12361 agcggtattc acactcagcg aacgttcatc gataggaatt aaaaccccaa aaagattaaa
    12421 aaaacaccac aaaacggat
//
LOCUS       DAJTYM010000102        12424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTYM010000102 DAJTYM010000000
VERSION     DAJTYM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..285
                     /locus_tag="OO575_003172"
     CDS             <1..285
                     /locus_tag="OO575_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6666033.1"
                     /translation="AVAVWLSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWM
                     ENTIFPAMKDIPVLSGLIDTMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            296..1036
                     /locus_tag="OO575_003173"
     CDS             296..1036
                     /locus_tag="OO575_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6666034.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1095..1481
                     /locus_tag="OO575_003174"
     CDS             1095..1481
                     /locus_tag="OO575_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6666035.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1490..1819
                     /locus_tag="OO575_003175"
     CDS             1490..1819
                     /locus_tag="OO575_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6666036.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1791..4832
                     /locus_tag="OO575_003176"
     CDS             1791..4832
                     /locus_tag="OO575_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6666037.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4832..5161
                     /locus_tag="OO575_003177"
     CDS             4832..5161
                     /locus_tag="OO575_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6666038.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5161..5859
                     /locus_tag="OO575_003178"
     CDS             5161..5859
                     /locus_tag="OO575_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6666039.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5864..6607
                     /locus_tag="OO575_003179"
     CDS             5864..6607
                     /locus_tag="OO575_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6666040.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6544..7146
                     /locus_tag="OO575_003180"
     CDS             6544..7146
                     /locus_tag="OO575_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6666041.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7207..10686
                     /locus_tag="OO575_003181"
     CDS             7207..10686
                     /locus_tag="OO575_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6666042.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10754..11353
                     /locus_tag="OO575_003182"
     CDS             10754..11353
                     /locus_tag="OO575_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6666043.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11417..>12424
                     /locus_tag="OO575_003183"
     CDS             11417..>12424
                     /locus_tag="OO575_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6666044.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGI
                     NG"
BASE COUNT         2926 a         2953 c         3974 g         2571 t
ORIGIN      
        1 gccgtggcgg tgtggctgag tgatgcgcag tacaccggaa aaatgacgga tgaggacgac
       61 tggcaggctg ttctgcatat tgccgttttc gtccgtgcac aggcgccgga ttctgagctg
      121 gatacgtgga tggagaacac catatttccg gccatgaagg atattccggt gctttccgga
      181 ctcatcgaca ccatgatccc actcggtttt aactatcaac gtgataatga gatggccacc
      241 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
      301 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
      361 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
      421 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
      481 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
      541 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
      601 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
      661 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
      721 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
      781 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
      841 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
      901 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
      961 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
     1021 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
     1081 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
     1141 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
     1201 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     1261 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctgagc
     1321 agtctcagga tgttgagacg ctttgcgcat ccattatgac aacatggtcg tatgatgcgc
     1381 tgggcgcggg ggcggagagg gttctgtcgc tgagcggtat ggggaccatt gagaatgccg
     1441 gggatgatga tcatgaggcg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     1501 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggatatgtc
     1561 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     1621 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     1681 cgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     1741 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     1801 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     1861 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     1921 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcaggcg gcttcttcgg
     1981 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     2041 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     2101 gcgaggaaca ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct
     2161 tttatcgcca gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta
     2221 ttcagcagca gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg
     2281 cgctgatttc ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca
     2341 cccggcagaa agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt
     2401 cgtcttctga gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag
     2461 tgtatatccg caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg
     2521 cggcagcccg gcgggagctg ggggtgttaa tcagtcagat ggcgcgaggc aattttggtg
     2581 cgctgagggg atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt
     2641 cgccgaaagg catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc
     2701 tgggtaaagc ctggcatgac ggccggaagg agggcgagaa atttaaccgt cagctggcgc
     2761 tgacgggaca ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat
     2821 ccgggaatgg tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg
     2881 gggcatttca tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat
     2941 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
     3001 taaatgccgc gaaggcgctg gacaatgcgc tgcatttttt gaatgccact cagcttgagc
     3061 agatacgcgt ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg
     3121 cgctggcaga ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg
     3181 gtagtacgct gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata
     3241 ttggtcgtga agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg
     3301 cgaaaaaata tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta
     3361 acgatcttca ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac
     3421 ggaattacca ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga
     3481 atgaaacgga agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg
     3541 ccgaccaggc tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca
     3601 ttaagaaaaa tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca
     3661 gtcagcagca ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg
     3721 ccactgaaag gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg
     3781 acctggacgg gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc
     3841 tgattcaggc gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc
     3901 ttaacgacct gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg
     3961 cactgcgtga gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc
     4021 agcgctacca ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg
     4081 agaatgacag tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg
     4141 tgctgaaggg gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg
     4201 aagtggcgca gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg
     4261 atgcggataa tgcggcggga acggcagaac agatgtttac agtggcattc agcagtgccg
     4321 gtaatgcact ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt
     4381 ccctgttgtc agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg
     4441 gcgtcggttc tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg
     4501 atttgagtcg ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag
     4561 gcgcgggtgt gatgggggaa gcgggacctg aagccattct gccactgcgt cgcggtgctg
     4621 atggtaagct gggggttgtg gcggatactg gtggttcagg catggtgatg tttgccccgc
     4681 agtacaacat tgagatcaat aacgacggca cgaacgggca gataggtccg gctgccctga
     4741 aggtggttta tgacctcggg aaaaaagcgg ccgcggactt tatgcaacag caggcccgtg
     4801 atggtggccg gttaagtgga gcataccggt aatggagacg tttcactgga aagtgcgccc
     4861 ggatatgaat gtggtatcag agccgaaagt ggtgacagtg aagctgggcg atggttatga
     4921 acagcgtcgt gcggcgggac tgaataacca gttgtcgact tacagcgtga cgatacgtgt
     4981 tcgtaaaggt gaacacccat ctttaaaagc ctttctggaa cggcacggtg gcgtccgcgc
     5041 atttcagtgg acgccacctt atgactggaa actgatcagg gtggtttgtc gtaaatggtc
     5101 ggcaagcgtg ggggcgttat gggtgactgt aacggccgat tttgaacagg tggtgaactg
     5161 atgcaggata tccggcagga aacactgaat gaatgcaccc gtgcggagca gtcagcccgg
     5221 gtggagctct gggaaatcga tctgacagag gtcggtggtg agcgttattt tttctgtaat
     5281 gagcagaacg aaaaaggtga gccggtcacc tggcaggggc ggcagtatca ggcatacccc
     5341 attcaggggt cggggtttga actgaacggc aggggctgtg ctgcccgtcc gacactgacg
     5401 gtctctaacc tgcacggcat ggtcaccggg atggcggaag atctgcagag tctggtcggc
     5461 ggaacggtgg tccggcgtaa ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac
     5521 ggaaacagcg acgccgatcc ggaacaggag gtgatcagcc gctggcgcat cgagcagtgc
     5581 agcgaactga gcgcggtcag tgcctctttt gtgctggcca caccaacgga gacggatggc
     5641 gcggttttcc cggggcgtat catgctggcg aatacctgta tgtggactta ccgttctgat
     5701 gagtgtggtt acacgggcag ggcagtggct gacgagttcg acaaaccaac aacggatatc
     5761 cggaaggaca aatgcagcaa gtgtatgcgc gggtgtgagt tgcgcaacaa taccggtaat
     5821 ttcggcggtt tcctttccat caataaactt tctcagtaaa tccatgacac agacagaatc
     5881 agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgtggct tcgtggtgag
     5941 aacgccggag ggagacaggt atcttcccag cgagaatatc tccggtgagc cggaggaacg
     6001 gttccggatg gctccggagg actggctgcg ggcacaaatg cagggtgaga ttgtggcact
     6061 ggtccacagt catcccggtg gtctgccctg gctgagtgag gctgaccggc ggctgcaggt
     6121 gcagagtgat ttgccgtggt ggctggtctg ccgggggacg attcacaagt tccgctgtgt
     6181 gccgcatctc accgggcggc gctttgagca cggggtgacg gactgttaca cgctgttccg
     6241 ggatgcttat catctggcgg ggattgagat gccggatttt catcgcgggg atgactggtg
     6301 gcgtcacggt cagaatctct atctggataa tctggaggcc acagggctgt atcaggtgcc
     6361 gttgtcatca gcacaaccgg gcgatgtgct gctgtgctgt tttggttcat cggtgccgaa
     6421 tcatgccgcc atttactgcg gcgatggtga gctgctgcac catattcctg aacaactgag
     6481 taaacgagag aggtataccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg
     6541 ggaatggcac gcatctgcct ttacggggat ttgcaacgat ttggccgccg catcgacctt
     6601 cgtgtgaaaa cgggggctga agccatccgc gcactggcca cacagctccc ggcgtttcgt
     6661 cagaaactga gtgacggctg gtatcaggtg cgcattgccg ggcgtgatgc aggtgaaacc
     6721 gaattgtctg cccgtcttaa tgagccgctg gcaaatggtg cagtgatcca catagtaccg
     6781 cgtctggtgg gagctaaaag tggcggtgtg tttcaggcgg tgctgggggc agctgttatg
     6841 gcggttgcta tatggatgcc gggggtagga attatggcga gtaatctgct gttttctctc
     6901 ggtgccagta tgacgcttgg cggtgttgca cagatgctgg caccgaaagc cagaactccc
     6961 cgtacacaga caacggataa cggcaaacag aacacctgtt tctcctcact ggataacatg
     7021 gttgcccagg gcaatgttct gcctgttctg tacggtgaaa tgcgcgtggg gtcgcgggtg
     7081 gtatctcagg agatcagcac ggcagacgaa ggggatggtg gtcaggttgt ggtgattggt
     7141 cgctgatgca aaatgtttca tgtgaaaccg cctgcgggcg gttttgtcgt ttatggagcg
     7201 tgaggaatgg gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
     7261 aaatccacgc agttgctgag tgtgattgat gccatcagcg aagggccggt tgacggtccg
     7321 gtggatggat taaaaagcgt gctgctgaac ggtacgccgg tcctggatag cgaggggaag
     7381 acaaactttt ccggtgttac ggtggtgttc cgcgccggcg agcaggagca gacaccgccg
     7441 gaggggtttg aatcttccgg ctcagagact gtgctgggta cggaagtgaa atacgacacg
     7501 ccgatcaccc ggaccatcac gtcggcaaac attgatcgtc tgcgcctgac cttcggtgtg
     7561 caggcactgg tggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     7621 gttcagatac agcgtaacgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     7681 accacttcgc agtatctggc ctcggtggtg gtgggtaacc tgccgccgcg cccgtttaat
     7741 atccggatgc gcaggatgac accggacagc accacagacc agctgcagaa caaaacgctc
     7801 tggtcgtcgt acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgctggtc
     7861 ggcgtgcagg tggattcaga gcagttcggt aaccagcagg tgagtcgcaa ttatcatctt
     7921 cgcgggcgca ttctgcaggt gccgtcgaac tataacccgc agacgcggca atacagcggt
     7981 atctgggacg gaacgcttaa gccagcatac agcaacaaca tggcctggtg tctgtgggac
     8041 atgctcactc atccgcgcta cggcatgggg aaacgtcttg gtgcggcaga tgtggacaaa
     8101 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag tgccggacgg atttggcggc
     8161 acggagccgc gcatcacctg taacgcttac ctgaccacac agcgtaaggc gtgggatgtt
     8221 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     8281 ttcgtgcagg accgaccgtc ggataaagtg tggacctata accgcagtaa tgtggtgatg
     8341 ccggatgatg gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg ccataatgtc
     8401 gttgaggtga actggattga tccggacaac ggtcatgaga cggcgacaga gcttgtggag
     8461 gacacgcagg ccattgtccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     8521 accagccggg gacaggcgca ccgcgccggg ctgtggctga ttaaaacgga actgctggaa
     8581 acgcagaccg tggacttcag cgtcggcgca gaagggcttc gccatgtacc gggtgatgtc
     8641 attgaaatct gcgatgatga ttatgccggt atcagcaccg gtggtcgcgt actggcggtg
     8701 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccgtc ctccggtacc
     8761 acgctgataa gcctggttga cggaaatggc aatccggtca gcgtggaggt tcagtccgtc
     8821 accgacggcg tgaaggtgaa agtgagccgg gttcctgacg gcgttgcaga atacagcgtg
     8881 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     8941 aacgacgacg gcacgtatgc catcaccgcc gtgcagcatg tgccggaaaa agaggccatc
     9001 gtggataacg gggcacactt tgacggtgac cggcgcggca cggtgaatgg tgtcacgccg
     9061 ccagcggtgc agcatctgac cgccgaagtc accgcagaca gcggggaata tcaggtgctg
     9121 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     9181 gcagcggacg acggcagtga gcggctggtc agcacagcca ggacgacgga aaccacatac
     9241 cgcttcaggc agctggcgct ggggaattac agtctgacag tccgggcggt aaatgcccgg
     9301 gggcagcagg gcgatccggc gtcggtatcg ttccggattg cggcaccggc agcgcctgtc
     9361 actattgaac tgataccggg gtattttcag ataacggtgg tcccgaaact ggctgtatat
     9421 gacccgacgg tgcagtttga gttctggttt tcggaaaagc ggattgcgga tatcaggcag
     9481 gttgaaacca gcgcccgcta tcttggcacg gcgctgtact ggatagccgc cagtatcaat
     9541 atcaggccgg gccatgatta ttatttttac gttcgcagtg tgaacaccgt tggcaaatcg
     9601 gcatttgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct gagtttttat
     9661 aaagggttga tcaataaaac gcatctcggc aaggagttgt ggacgcagat tgataacggt
     9721 cagcttgcgc cggacctgac tgaaatcagg acgtccatta cgaatgtcag caatgaaatc
     9781 acgcaaaccg tcaataaaaa actggaaaat cagagtgctg caatccagca gatacagaaa
     9841 gttcaggttg atacaaataa taacctgaac agcatgtggg ctgtgaagct gcaacagatg
     9901 aaggacggac gcctttatat tgcgggtatc ggtgccggta ttgagaatac gccagcagga
     9961 atgcagagtc aggtgctgct ggcggcagac aggattgcga tgattaatcc tgcgaatggc
    10021 aacacaaagc cgatgtttgt tggtcagggc gatcagatat tcatgaacga cgtgttcctg
    10081 aaacgcctga cggctccgac cattaccagc ggcggtaatc ctccggcatt ttccctgaca
    10141 cctggcggac ggctgacggc gaaaaatgcc gatatcagcg gtaacgtgaa cgcgaactcc
    10201 gggacgctca acaacgtcac gattaataag aactgtcggg ttctgggaaa attgtccgcg
    10261 aaccagattg aaggcgatct cgttaaaaca gtgggcaaac ctttcccacg ggactcccgg
    10321 gcaccggaga ggtggccatc agggaccatt accgtcaggg tttatgacga tcagccgttt
    10381 gatcggcaaa ttgttattcc cgcggtggcg tttcgcggtg ctaaacatga gcggaagaat
    10441 aacaatattt attcgtcatg ccgcctgata gtgaagaaaa acggtgctga aatttataac
    10501 cgaacgaccc tggataatac gctgatatat acgggtgtta ttgatatgcc tgccggtcac
    10561 ggtcacatga cgctggagtt ttctgtatcg gcatggctgg taaatggctg gtatcccaca
    10621 gcaagtatca gcgatttgct ggttgttgtg atgaagaaat ccacagcagg tatcagtatc
    10681 agctgaattt tataacccag atacgggcac cagaaatggt gcctttttta ttgcagaaaa
    10741 gcgagaggta attatgcgta aactttatgc tgccattttg tccgcagcca tctgtctggc
    10801 tgtatccggt gcgcctgcat gggcgtctga acatcagtcc acgctgagcg cggggtatct
    10861 tcatgcctcg acgaacgctc ccggcagtga tgatctgaac gggattaacg tgaaataccg
    10921 ttatgagttt acggacacgc tggggctggt gacgtcattc agctatgcca acgctgaaga
    10981 tgagcaaaaa acgcattaca gtgatacccg ctggcatgag gattccgtgc gtaaccgctg
    11041 gttcagcgtg atggcggggc tgtctgtgcg cgtgaatgaa tggttcagcg cgtatgcgat
    11101 ggcgggcgtg gcttacagcc gtgtgtcgac cttctccggg gattatctcc gcgtaactga
    11161 caacaagggg aaaacgcacg atgtgctgac cgggagtgat gacaaccgcc acagcaacac
    11221 gtctctggcg tggggggctg gcgtgcagtt taacccgacc gaatccgtgg ccattgacct
    11281 tgcttatgaa ggttccggca gtggcgactg gcgcactgac ggtttcatcg tgggtgtcgg
    11341 ttataagttc tgattagcca ggtaacacag tgttatgaca gcccgctggt tcaggcgggc
    11401 tttttgtggg gtgaatatgg cagtaaagat ttcaggtgta ctgaaagatg gggcgggtaa
    11461 acctgtcgta aattgtgcga ttgaactgcg ggccagaaga accagtccga ccgttgtggc
    11521 acacgttgtt gccacttgcg tgacggacaa taacggtgct tatgtgattg aggctgagcc
    11581 ggggtattac gaggttgcgc ttcactgtaa cggctggcag ccaacccgtg tcggggatat
    11641 tgatgtggca ccgactgatg caccggggac actgaacgcg tttctgaatg caccaaagga
    11701 tggtgattta cgtccggagg tgatgaagcg ctttgaggaa atggtggcgc aggcgcagca
    11761 gagtgccggg gctgcagccg gaaacgcaca gcagacggcg caggatgtgg cggcagccgc
    11821 aacggcccgt gatgatgcac aacgttttgc ggagaaagcc cgacaggatg caaccgtcac
    11881 agctgaggac agaaaggcca ctgcggaaga tgtgacaagc acaggagcaa atgcagccgc
    11941 agccggacag agcgcacagg atgccgcagg ttatgcccgc gcagcagaac aggccaaaaa
    12001 tgacattgat gctgcgctga ccggcactct gaaaatggct aaccatctgt cagaaatcgc
    12061 agcagcaggc gaaaaggcac aacagaagtc ccgggataat ctggggctga aaagtgcggc
    12121 cacgatggaa gcacagagcg acatttacga ccggacaaaa ggccgtctgg cgatacccgg
    12181 cgcattcggc tttgggtgtg cttttctgcc tgaagatgtt atccgttttg acactaagag
    12241 tgatttcctg gcctgggtaa ggaatgcgct gccaggtgaa tattccgttg ctggccccta
    12301 cggcatcatc atacccgaca cacggtttga aggggtgctc agcatccggt ggactgatgc
    12361 acgccctgag acaacagaac cgcggtacag agccaaatcc cttacttttt acggcattaa
    12421 cggc
//
LOCUS       DAJTYM010000103        12404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTYM010000103 DAJTYM010000000
VERSION     DAJTYM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            289..365
                     /locus_tag="OO575_003184"
     tRNA            289..365
                     /locus_tag="OO575_003184"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Arg,seq:tct)
     gene            complement(411..1172)
                     /gene="envY"
                     /locus_tag="OO575_003185"
     CDS             complement(411..1172)
                     /gene="envY"
                     /locus_tag="OO575_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6666045.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1355..2245)
                     /locus_tag="OO575_003186"
     CDS             complement(1355..2245)
                     /locus_tag="OO575_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6666046.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2246..5214)
                     /gene="nfrA"
                     /locus_tag="OO575_003187"
                     /pseudo
     CDS             complement(2246..5214)
                     /gene="nfrA"
                     /locus_tag="OO575_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            complement(5201..7438)
                     /gene="nrfB"
                     /locus_tag="OO575_003188"
     CDS             complement(5201..7438)
                     /gene="nrfB"
                     /locus_tag="OO575_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6666047.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7588..9030)
                     /gene="cusS"
                     /locus_tag="OO575_003189"
     CDS             complement(7588..9030)
                     /gene="cusS"
                     /locus_tag="OO575_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6666048.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(9020..9703)
                     /gene="cusR"
                     /locus_tag="OO575_003190"
     CDS             complement(9020..9703)
                     /gene="cusR"
                     /locus_tag="OO575_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6666049.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            9860..11242
                     /gene="cusC"
                     /locus_tag="OO575_003191"
     CDS             9860..11242
                     /gene="cusC"
                     /locus_tag="OO575_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6666050.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            11266..11598
                     /gene="cusF"
                     /locus_tag="OO575_003192"
     CDS             11266..11598
                     /gene="cusF"
                     /locus_tag="OO575_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6666051.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            11614..12333
                     /locus_tag="OO575_003193"
                     /pseudo
     CDS             11614..12333
                     /locus_tag="OO575_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2982 a         3168 c         3243 g         3011 t
ORIGIN      
        1 tgatgatagg atgagtatcc ataatgatca ccgacgttgg tttcatagtt accagtctca
       61 taggagcgga caattttccg ttagggcggt aaattgtact ttgatacatg aaaatacggg
      121 ttttcttgat tcagacgcgc agcggtgtgc gtttgtttgc cgctatagcg aaataaatca
      181 gaaaatcaga cgcggtcgtt cacttgttca gcaaccagat caaaagccat tgactcagca
      241 agggttgacc gtataattca cgcgattaca ccgcattgcg gtatcaacgc gcccttagct
      301 cagttggata gagcaacgac cttctaagtc gtgggccgca ggttcgaatc ctgcagggcg
      361 cgccatttct tcttcattta tgcccgtctt atccgtttcc gctttgccct tcaccacatc
      421 actttttgtc gctgtttggc gagataattc aacggtgtca ggctgtaaaa cgccttaaaa
      481 acagagataa agtacgacgt gctgctatag ccacataatt gcgccacctg agtgatattt
      541 ttgttatcca tcaataacat ctgtacggcg taacgcatac gacactctgt gacaatctgg
      601 ctatagctgg tattttcgtt ttttaatttc tttttgagca ggctggggct taaacatagc
      661 gaactggcga caattcgcag attccagtaa tgctgaatat cgctttgaat aatgcggcag
      721 acgctgtcgc ggacgctgct gcgtaagata tacatcagta gggcaataaa ccgcgattgc
      781 tcaagaaagt tagacaatac ggtaaaaagt aatgcgcgcg tcaactccgc ttcgcagggt
      841 ttgctggatt gctgcttgct gtggttggtg gctagccgga acacttccgg tgtacggcta
      901 tgacaagcga taaccggggt tgctagccgc gaccagggct ttacacaggt taatttctta
      961 tttaaaaaca acagataatc gttgatgatg ttacggttga tgtgggtgat taatacgtga
     1021 tttaacgttg agagttcaat gacgttattg ttgcacgcga aggcggccag gtagtttttc
     1081 ggaagggtaa acgtagcatg gttattgacg cttacctcta cctctttttc ggtcaggatc
     1141 accacgcaag gttcactgct gctcaattgc atttcgcact cctcagatat cagaaactcc
     1201 gctcaaagga tctatgcttc ctgcatgagt gatcggcccg ttcgccgata acgatcttct
     1261 ttctttagca tgctttttag caattaatct tgatggaatt ctgacgagag cgaaagaggt
     1321 aagccaggtc gtacccgact tacctggagg ggatttaata ctcgagaatg ccgtgcgcga
     1381 cgggcaaata gcgcagagag aaatagagca tgtctttacc gcaagaggaa cgtttcgcta
     1441 acagcgaggc aatttctgcg tccggtttcg gtttcgctgt aaaggttttg cctttgccgg
     1501 cgacaaacag ttcataaaca atgccgcttg ctggggcgga gctttggcaa tcggcgctgg
     1561 cctgtagata acgttcacgc tgttcagcgg taagtttatc cacgccgctg ccatcctgta
     1621 cccagacatt aactccggtt gctttaactt gttccagcag ttggcgatag ccatcgggcg
     1681 acatgtttcc ggcgaaaaaa ctactgatat aaaccggttt tgctgaaaca tcgctaatca
     1741 gccgctgcgc gttgtttaac catgttagca aaggttgacg ggcggcttcg ctgcgccagt
     1801 tcaggtcgtc aatttccgcg ctgatgtacc agccatccgg cgttatgcca ggcgcggcgc
     1861 tccataatct ggcttgctgg agatcggcag ccagcaggcg attaagatag ctttccagcg
     1921 ctgcagacga ctgtttctgg tgcataaaaa attccggatc ggcgttcagc ccgacaatca
     1981 gcttcaggcc agtctgttgc gcagctgcgg cacgcttaaa caataacgtg cgctgttctg
     2041 gctgggtaaa tgcatcgccg taacgggtcc attgcaaaac cagggtatcg aagccttgaa
     2101 aacgtaactg actcatcagc ccctgccact gggtatcggt aacctgacta tctcggtttt
     2161 gtggttgcca gataatacct ttcatcgcaa aggaaaaagg gctgaccaaa agcagtgtca
     2221 gcaatacgaa aatgaactta cgcatttacc agtgcactcc aatggtgaga aacgcgttgt
     2281 tgcgctctcc gttacgttga ttaatcgcct taaaggtatg ttgatactcg acgccgagac
     2341 tgactttgtg cggccaggcg tcgtagtgcg tctcgccggt ccaaatattc cagcggaccc
     2401 cgacaccgcc aagctgcgcg ccctgagtgc ctttatcacg atagccgttg tcctgaacgt
     2461 gagcgtaagg ctcaatagtc tgtccgttag ctaccttctg atgccagctg acgcgataat
     2521 ctgccgtcca cgcctgaata tcctggcgga tatattgcgc cgcatcgagg tacaggtttt
     2581 gggcaaacca gcctgaaccg ttcgggtgcc attcgtcgct gtatttgccg ccattaaaga
     2641 atgaggcgct ggcgcgcagc atggtatcgg atgcgccatt ttgcccgttc agcggcaact
     2701 gctgttcgac ggcgaggaaa aagatctgat cgcgcagcgg cttccagcgc agaccggtgc
     2761 cggacatcgg atttttcacc ggcatcatca ccccgttttc tccggtatcg gcaaagacgc
     2821 ggctataaac tgagagcagg tcgccttcca gcagcatatt gcgtccgatg cggtactcgg
     2881 cttccagttg tccgtagcta cgatagcttt tcccaggcgc tgcaccgccg acattattgt
     2941 tagcggtact cattgcgccg gaacgcaagc cgatggaaga atcgaaactg aacgtccagc
     3001 ggcgaccgac ctcctcatgc aaacggcgga aattgaagcg ttgctgattt tgctctgggg
     3061 tcagtggggt tatgcctgat tatcaatgtc atcaatcacg agccgggcgt agtgctgcgt
     3121 cgcaggcatg tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc
     3181 gtccggaagc cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct
     3241 atcccacaag gcgtaaccaa gcgctgcctg ggtgttgcta ttatttggtt ccagttccag
     3301 cgcggcgcgc aaatcactca ccgcggccgg aacattatga cgttggcgat aaattgtcgc
     3361 ccgcgcaacg taagagttgg cagaaggggc aatattgatt gagcgcgtga gatcgttcag
     3421 tgcgagttct ggctgagctg ggatataacg tcgcgcatgc agccaccagt attgagcatt
     3481 gtttcccagc tcacgttgtt ctgcctgttg aagccagcga tcgcgagctg cgccatttcc
     3541 cgctgcctga gcggtgttgg cggcggcgag cagatcatca ttgctcatgt cgtgaagact
     3601 gattttctgc caggccgcca gtgcggtggc gtagtcctca acctgatacg cctgataggc
     3661 taccgcacga tgttgccagg cgctcggttg tcgttgttcg gcctgaagcc atgcatacaa
     3721 cgccacaccg ggtagcgtgt cccgataaca ctttgccaga cggttccagg cggcagcatc
     3781 gtaggaaggc gacatatcgc ccagcaagcg aactattgcc gggcaattat ctgcaatacc
     3841 cggcaactga ctttgccact gacgttgctc cgccagcggt aagggtttcg ataaaatcgc
     3901 caccttcgcc ggcgttgcta ggtaaggatg actttccagc agagacgcca gtcgcgccat
     3961 taaagtctgg ctgacacgcg catcgccctg gaaaggatag cgttgcagca ataaatcggc
     4021 agcttcgcgt gactgctcgt tctgcatcag ttgccaggtc agttgatcca ggcggggaag
     4081 atttgtcggt tcttgctgat acagcaatcg tgccagacgc agagattcag ccttgttacg
     4141 ggtcgccacg ctgacagcat aacgctcctc aagcatttca ttggcgggga gggtggcgag
     4201 cagtttttgc gctgcgtcgt actgaccttc ttttaacagc accggtagcg tcgcgccaac
     4261 aacatactgg cggttatcgg caaactgcac cgtataattc gccaatgcct gaacggggtt
     4321 ggcgctgtat ttagataaca gatagagcca acttttctct tgtgcgcccg tggtaaacag
     4381 cggcttattt tcaatgagat aatgctggag gcgtgctttt tcgccacgat aagccagcgc
     4441 ggtcgcgtaa gtaatatatg actgaggatc ggtgaagatc ccctgtgatt gcagtgccag
     4501 gatccgatcg tccagctgcc cggcaagaag cacgtcaaac cactgacggc gttctgccgc
     4561 gcttaatgtg ttctgctggc gtgcttcatt gtatagcgta tctgcctggg atcattgttt
     4621 caggtagatt gcccgttgca gcagatcggt tcgcagcgtt tttccttccg gcgatgcagc
     4681 aaacgtcgca tcgttcagtt gcgctctggc gacaggtaat tgtgccagcc gcagggcatt
     4741 ctgcccgact tcactgcgac aacgcagggt cggcgcagcg tcgcccgctt tttgctgggc
     4801 aagcagttct tcaacagtcg taacgctttt cacttcaact ggaatagccg ccagactgcg
     4861 ctcaagtcgg gcatctcctg ggtgacgttt cagttgatcc tcaagcaaca gccgcgcccg
     4921 gtcatcatga ccaaaatgac gataggcttc cgcaaggtat aaagtcagcg gaatattatc
     4981 cggcacctgt tggtgtatat attcaaattc gcggatggcg gttgcttcgt cgttattttt
     5041 ctgtgccttc agcgccttat cgagacgggg ataaataaca aaatggcgat aatcgctcag
     5101 ccccagctct tctgcgctgg tgccgatatt gtctgcgagt gcgctggtac tcaataaaga
     5161 cgtcagcagt aaaccagacc atccgatgac gcgattaagg ttattctcct tcattttcgg
     5221 actccagttg cgcaacctgt tctgtgttta aacctgcttt gagtaatagt gattgcatcg
     5281 aaacttgtaa ttcgcgttga attgtcagga cgcgatccaa cgtttcctgg ctgataacgc
     5341 cttcggtgac caaaaacttg ccgagcggca gagaactgcg ttcatggcgc aataacaaca
     5401 cgttaattgc tgaacgatta atatgaccga gcgtggtcag tatttcggcg aacaggaact
     5461 gatgcggcac atattgccgc cagatttcac cggtctgctg ttccgtgagc cactgatgct
     5521 gaaccgcatt gtacaacatt gcccgcggat cgtgaccgcg tcggcgtgca taccagtggc
     5581 gtaaccctgt gacaatttgt ccccgcagaa caatgacgta acgcactttg cgtccgactt
     5641 tacgcgtcag ggccgccagc gaaaccgggt caataccatc ttcactgccg acaattaact
     5701 catcattttc cagacgcagc ggcagtactg cataatgcag cgctacggag gctggcattt
     5761 cggcaatcag cgaggacggg atctgccagg catcgatgga ttcccacgcc acgccgtttt
     5821 gctctgccag cgcctgtgcc agctgctcgg cgctaatcag cccctgcatc agcattgaac
     5881 cgcccaggcg tagaccttcg acgcgattac gcagtgctgt atcgagttgt tcttcagtga
     5941 tgacctgatt ttccagcaga atttgaccta acgggcgcaa cgagcgggta tcgccagtga
     6001 cgctggggaa gtcatgcgtt gttttatccc acgcgacgcg acgcggatcg ccgtgctgaa
     6061 gaacctgttt cagcacgcgc cagttagcca taaagttaat caggttgccc cagaaaagac
     6121 gcaggacgga aagcaggccc tgcgtgaggc cgtagtagcc agtgacgaaa atcactcgct
     6181 gcacgatgcg gttaaccatc aagccaaagt ttagccacag cagggtcatt aaccatgcgc
     6241 tgccgctaaa aatagaaagg aaatgccagg catcgggcca caaactttca tacgccagca
     6301 acagcaaaag ctggatcatc accagcatcg cgaggaagct gacaaagtta ctgattgccc
     6361 ctttgcggtc gcgccagaga aagtagttca gcgtcaggct ggaggtccat ttatgggttt
     6421 taaagccctg gaaaacaatg ccgatgatcc agcgagattt ttgtcgaacc gcagtcgaaa
     6481 aggtatcggg gaaatattcg cgcacgcaga tcatgtttga cgtccgtgcg tgctgtaaaa
     6541 atttacgctg ctcgcgttct ttggcttcgt ccaccaccgg aaaacggaca aaaatttccg
     6601 tcataccttt ttctttcagg cggaagccaa tgtcgtaatc ttcagtaaga ctctgcacgt
     6661 cgaaagcaat accgttaccg tcagctaaca gtgcggtcac ggcgcggcgg ctgaaacagg
     6721 tgccgacgcc tgcgctgggc acttgtccgg cgagggcttc acgcaccgga acatctttgc
     6781 catgcagttc tgaaaactca tcaatgtaag tcatgctggt gaagtgcgtc cattcgcgtt
     6841 caaacggata cactgggatc tgaatcagat ctttacgctc gaccagatag ttgaacagac
     6901 gcaattccat cggtgaaatc acatcttcgg cgtcatgcag aataaaacca gcaaaagcga
     6961 aattggcgct acgttcaaac tgggtgatgg cgtccagcac gttgttcagg cagtcggctt
     7021 tgctggtggg accaggacgc gcgcagacta ccttatgcac gttcggaaag cgagcgcaca
     7081 cttcgtcaac atcacgttga gtatcggggt cgttggggta ggtgccaaca aagatatgat
     7141 agttttcgta gtcgagcgtg gtcgccgcca gctcggccat attgccgatg acgcccgttt
     7201 cattccacgc cggaaccata atcgctaacg gtttttcatc tggtttatac agttcgcggt
     7261 aactcattcg cgggtagcgg cgataaacac tcaacttgcg tttaatgcgg cgtacccagt
     7321 agacgacatc aataaaaaaa tcgtccagcc cgctgatgaa catgatgacc gctaacgtta
     7381 tcgcgattac ttttaagccg tagagccagg tagcaaaaac atcaagaagc cagtccacac
     7441 aaaaacctta cattaacgct ggttatgttt agggtggcgt atattaaggt tttttatgaa
     7501 ttgtgacagc tttttaccat taatatgtat gactattgcg gcacgttatt tttacactgg
     7561 ttataaaagt tgccgtttgc tgaataatta agcgggtaat gtgataacaa accttgtccc
     7621 ccgcgcatct gacgttaccg caaccgtgcc tttatgcgcg acaacaatcg atttcactat
     7681 cgccagccca ataccgctac cttcaccttt tcgctggcgg gaggggtcaa cgcgatagaa
     7741 acggtcaaac aatcgcggta agtgctcggg cgcaatgggc gtaccggggt tttcgacgga
     7801 aacttgcacc tggtgatcga ccgtctgaca gcgtactaca attgtctctc tggttggcgt
     7861 ataacgaagg gcattagaaa gcaggttgct tagcgcccga cgcagcatca acggatcgcc
     7921 cgcaacctga cgtttgtcgc caacaaaccg caactccacg ccgcgatctt ccgctagcgc
     7981 ctcgaaaaaa tcgaacactt tgccgacttc atccgccagg ttgagcattt tcttttcggg
     8041 gattagctgg ttgttatcgg cctgagcgag aaacagcata tcgctgacca ttttcgccat
     8101 tcgcgtcagc tcttcgagat tagagtagag cacatcttcc agctccttct ggctgcgcga
     8161 ctggctgagg gcgatttccg tttgcgtaat gagattagta attggtgtgc gaatttcgtg
     8221 ggcgatatcc gctgagaaat tggactggcg agtaaagaca tcctcaatac gctcgatcat
     8281 atggttgaac gacagtacca gctgttccag ctcaatcggc acggtctgcg ggtcaaggcg
     8341 aacgtcgaga tctttcgagg tgatattctg gatttgacgg ctgacgctgc ggatcggcgc
     8401 gtgaccttta tgtaccgcca acagcacgat aaagacgatc aggatgctga ttaccgatgc
     8461 ggtcataata agtttattca tcaaatcatt tatgtaatga agatgaaaat cgatcgacag
     8521 cgcgatgtag agcgtataaa tcggtttgcc gtccaccaac gggccaaccg gcaagttaat
     8581 catccgccag ttgctgtgtt ccatatgccc gtgaccgtgg cctggcatca taatcgtcgg
     8641 gccggaaagg agatacacct cgccaccccg cgcgtcttta tcgggtatgg catcacgtgc
     8701 aaactcgcgg atatcaggcg caccggggga gtgatacacc gttttgccgt ggctatctgc
     8761 cagggaaatc aacacgttgg aataaccact gacgatatct tccagcgtca ttaagcgtcg
     8821 ggcttgcgtt tcgtcagggt gatttagtac ccgttcaagg gtggcgctaa tctcttttaa
     8881 atcattaatg tcctgctcgg caaaatgcac ttttactgag tggattatga tccatgcaaa
     8941 ggcgaaaaac gccgcgatgg tggcctggct gataaaaaag gtcaggcggg ttgccagcga
     9001 aaacgggcgc tgaaatggct tactgaccat ccggcacctc aagcatgtaa cccacgccgc
     9061 gcacggtttg aatcagcttc ggctcaaagt cgttgtcgat tttgccgcgt agcagcttca
     9121 ccgccacatc aatggcgtta gtgtcgctgt cgaaattcat gtcccatacc tgcgaggcga
     9181 taagcgagcg gggcagcact tcgccctgat ggcggagaaa aaactccagc agagtaaatt
     9241 ctttactggt cagagtgatg cgcgtgccgc tgcgggtgac tttgcggctg acgagatcga
     9301 ccatcagatc ggcaacctga aactgacttt cgataatcac cgccgccccg cgccgcagga
     9361 gagtgcgcac ccgcgccagc agttcagcaa aagcgaacgg tttcaccagg tagtcatctg
     9421 cccccaactc cagccccttg acgcgatgtt caatggtgcc aagcgcggta agcaacagaa
     9481 tcggcatccc tttattggcg gagcgcaaca tgcgcacgat atcccagccg ttcacgtctg
     9541 gcagcataat atcgaggatt atcaggtcat aatcaccggt catcgccaga tggtagccat
     9601 tcagcccgtt gtcggccaaa tcgaccacaa aaccggcttc ggttaaccct ttggtcaagt
     9661 attctccggt tttcttttca tcttcgacaa tcaacagttt catatttcct ccgcatgttg
     9721 cccgggcaat tctagagtag cgggatcaga tggcaatcgc ttattggcaa aatgacaatt
     9781 ttgtcatttt cctgtcaccg gaaaatcaga gcctggcgag taaagttggc gacataaaat
     9841 caccagaaat tatgagccta tgtctccttg taaacttctg ccattttgtg tggcccttgc
     9901 gctaactggt tgttcactgg caccggatta tcagcgtccg gtaatgcccg taccgcagca
     9961 gttctcactc agccagaacg gcctggttaa cgcagcggat aactaccaga acgtgggctg
    10021 gcgcaccttt tttgttgata atcaggtgaa gacgctgatt agcgaggcgc tggtgaataa
    10081 ccgggatttg cgcatggcga cgctgaaagt gcaggaggca cgggcgcaat atcgtctgac
    10141 cgatgccgac cgctacccac agctcaatgg cgagggcagc ggcagctgga gcggcaatct
    10201 taaaggcgat tcagccacga cgcgggagtt ctcgactggc cttaacgcca gctttgatct
    10261 cgattttttc ggtcgcttaa agaacatgag cgaagccgag cgccaaaatt atttagccac
    10321 tgaggaagcc cagcgcgcgg tgcatatttt gctggtttct aatgtcgcgc aaagctattt
    10381 caatcagcag ctggcgtatg cgcaattgca aatagccgaa gaaacgctgc gtaattatca
    10441 gcagtcatat gcgtttgtcg aaaaacaact gttgaccggt agcagcaatg ttctggcgct
    10501 ggaacaggct cgcggggtga tagaaagtac ccgcagtgac atcgctaaac gtcaggggga
    10561 actggcgcag gcgaataatg cattgcaact gttattggga agctacggca agctaccgca
    10621 agcgcagaca gtaaacagcg gcagcctgca aagcgttaaa ttaccggcgg gcttgtcgtc
    10681 gcaaatctta ttgcagcgcc cggatattat ggaagctgaa cacgcgttaa tggcggctaa
    10741 tgctaatatt ggtgctgcgc gtgcggcatt tttcccgtct atcagcctga ccagcggaat
    10801 atcaaccgcc agcagcgatc tgtcgtcatt atttaacgct agcagcggga tgtggaattt
    10861 tattcctaaa attgagatcc ccatttttaa tgccggacgc aaccaggcca atctggatat
    10921 cgccgaaatt cgccagcagc agtcggtggt gaattatgaa cagaaaatcc agaacgcctt
    10981 taaagaggtg gcagatgcgc tggcattacg tcaaagcctg gacgatcaaa tcagcgccca
    11041 gcagcgttat ctggcgtcgc tgcaaattac cctgcaacgg gcgcgggcat tatatcagca
    11101 cggcgcggta agttatctgg aagtgctgga tgccgagcgt tctttatttg caacccgaca
    11161 aactttactt gacctgaatt atgcccgtca ggttaacgaa atttatttgt ataccgcctt
    11221 agggggcggt tggcagcaat aatttttaac tccaggagag aataaatgaa aaaagcactg
    11281 caagtcgcaa tgttcagtct gtttaccgtt attggcttta atgcccaggc taacgaacat
    11341 catcatgaaa ccatgagcga agcacaacca caggtcatta gtgccaccgg tgtggtaaaa
    11401 ggtgttgatc tggaaagcaa aaaaatcacc atccatcacg atccgattgc tgccgtcaac
    11461 tggccggaga tgaccatgcg ctttaccatc accccgcaga cgaaaatgag tgaaattaaa
    11521 accggcgaca aagtggcgtt taattttgtc cagcagggca acctttcttt attacaggat
    11581 attaaagtca gccagtaacc caggtttaat gagatgaaaa aaatcgcgct tattatcggc
    11641 agcatgatcg cgggcggtat tatttcagcg gcaggtttta cctggtttga ccaggcggaa
    11701 ccgcacgcag aaaaaatgcc gaccgcagaa cgtaaaatct tattctggta cgacccgatg
    11761 tatcccaata cgcggttcga taaaccaggt aaatcgccgt ttatggatat ggatctggtg
    11821 ccgaaatatg ccgatgaaga gagttctgcg tctggtgtgc gcattgaccc gactcagacg
    11881 cagaatctgg gggtgaaaac ggctaccgtc acgcgcgggc cgctgacttt tgcccagagt
    11941 ttcccgacga atgtcagtta caacgagtat cagtatgcca ttgtgcaggc tcgcgctgcc
    12001 gggtttatcg acaaggtgta tccgcttacc gtgggcgata aagtacaaaa gggcacaccg
    12061 cttctcgacc tgaccattcc cgactgggtg gaagcgcaga gtgagtattt actgttgcgc
    12121 gaaaccggcg gtacggcgac ccagactgaa ggcattcttg agcggctgcg actggcggga
    12181 atgccggagg cggatattcg ccacctgatc gccacgcaaa aaatccagac tcgctttacg
    12241 ctcaaagcgc ccattgatgg cgtgatcacc gcgtttgatc tgcgcgcggg aatgaatatc
    12301 gccaaagata acgtggtcgc gaaagaggaa ggtaggttgc ttatctgtgt ctgtcattgt
    12361 ctagcattgt ccactgtagt taattaactg tctgttataa ctcg
//
LOCUS       DAJTYM010000104        12265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTYM010000104 DAJTYM010000000
VERSION     DAJTYM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..386)
                     /locus_tag="OO575_003194"
     CDS             complement(<1..386)
                     /locus_tag="OO575_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6666052.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSS"
     gene            627..1427
                     /locus_tag="OO575_003195"
     CDS             627..1427
                     /locus_tag="OO575_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6666053.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1505..2275
                     /locus_tag="OO575_003196"
     CDS             1505..2275
                     /locus_tag="OO575_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6666054.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2323..3669)
                     /gene="mltD"
                     /locus_tag="OO575_003197"
     CDS             complement(2323..3669)
                     /gene="mltD"
                     /locus_tag="OO575_003197"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6666055.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(3741..4496)
                     /gene="gloB"
                     /locus_tag="OO575_003198"
     CDS             complement(3741..4496)
                     /gene="gloB"
                     /locus_tag="OO575_003198"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6666056.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            4530..5252
                     /locus_tag="OO575_003199"
     CDS             4530..5252
                     /locus_tag="OO575_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6666057.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5249..5716)
                     /gene="rnhA"
                     /locus_tag="OO575_003200"
     CDS             complement(5249..5716)
                     /gene="rnhA"
                     /locus_tag="OO575_003200"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6666058.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            5781..6512
                     /gene="dnaQ"
                     /locus_tag="OO575_003201"
     CDS             5781..6512
                     /gene="dnaQ"
                     /locus_tag="OO575_003201"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6666059.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            6645..6721
                     /locus_tag="OO575_003202"
     tRNA            6645..6721
                     /locus_tag="OO575_003202"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6679..6681,aa:Asp,seq:gtc)
     gene            complement(6852..7898)
                     /locus_tag="OO575_003203"
     CDS             complement(6852..7898)
                     /locus_tag="OO575_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6666060.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(7912..8471)
                     /gene="tssG"
                     /locus_tag="OO575_003204"
                     /pseudo
     CDS             complement(7912..8471)
                     /gene="tssG"
                     /locus_tag="OO575_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8468..10351)
                     /gene="tssF"
                     /locus_tag="OO575_003205"
     CDS             complement(8468..10351)
                     /gene="tssF"
                     /locus_tag="OO575_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6666061.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10367..10861)
                     /gene="tssE"
                     /locus_tag="OO575_003206"
     CDS             complement(10367..10861)
                     /gene="tssE"
                     /locus_tag="OO575_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6666062.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(10858..11688)
                     /locus_tag="OO575_003207"
                     /pseudo
     CDS             complement(10858..11688)
                     /locus_tag="OO575_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(11675..>12265)
                     /gene="tagK"
                     /locus_tag="OO575_003208"
     CDS             complement(11675..>12265)
                     /gene="tagK"
                     /locus_tag="OO575_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HCR6666063.1"
                     /translation="SPNLSTVKTISQTAITSEAITEYLDLDWFRQQHLTSQNPFDIIP
                     VHGASSTGEGAEKGNALTRLYQEYREALLSPEQDLNRHADPFPFNEEPSSQDLDSLRD
                     LVTEAETLQDMVTGGLSIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAH
                     NTVLPDLTRKEHRIIGIDSHYRINPAQHGEIYHDKQ"
BASE COUNT         2799 a         3196 c         3294 g         2976 t
ORIGIN      
        1 gaggagacta gcgataaagt gacttccgga tagcgttcac ggaaaggctt aattaacggc
       61 atcagaaagt gcagcaccac tggagttgcg gcatcgatcc gtaacagtcc acgcggtgta
      121 ttacgcgtct ccataatttc tgattctgcc gctgccatct cctgcaaaat tgactgtacg
      181 cgacgaaaat aacgctcgcc ttcttccgtc aggctaagtt gccgcgtggt ccgattaagc
      241 aggctaacgc caagtttcat ttccagcttt ttcaccgccc ggcttaccgc tgagtttgct
      301 tgccctaatt gttccgctgc ccggctaaag ctgccgcttt cgacgaccga aacaaaaatg
      361 gcgagttctt ccgacgtggc tttcattttt gctcctgttg caaaatagat gagatatttt
      421 gaatttattt gtcattaaac catcaggatg cgtgatatgt catccgattt aatgttctca
      481 ataatgagca aatttctgac aggtgtaagc acttgcttac ataacaatat acaattgctc
      541 gttgaaagag tgagctaaaa tccctataac agtagaaccc tcccgagtgc ggaagggttg
      601 acgtaataga ggtttcaaag tcaaaagtgc gaaaaaacac ctatgccatg cgctatgttg
      661 ccggacaacc tgcggaaagg atcttaccgc cggggtcttt tgcgagcatc ggccaggcat
      721 taccacctgg agaaccgtta agtaccgaag agcgtattcg gatcctggtg tggaacatat
      781 acaaacagca acgcgctgaa tggttgtcgg tattaaagaa ctacggcaaa gatgcacatc
      841 tggtgttatt gcaggaagcg cagacaacgc cagagttagt acagtttgcg accgctaact
      901 atcttgccgc cgatcaggtg cccgctttcg tgctgccaca acatccttcc ggtgtaatga
      961 ccctttcggc ggcgcatcca gtgtattgct gcccgttacg tgaacgagaa cccattttgc
     1021 gtctggcgaa gtcggcactg gtgacggtct atccattacc tgacacccgc ctgttgatgg
     1081 tggttaatat acacgccgtc aacttcagtc tgggcgtgga tgtctatagt aagcagttac
     1141 ttcctattgg tgatcagata gctcaccaca gcggcccggt cattatggcg ggagatttca
     1201 atgcctggag ccgtagaagg atgaacgcgt tatatcgctt tgcgcgggaa atgtcgctgc
     1261 gccaggtgcg ttttaccgat gatcagcgcc gtcgggcgtt tggccgcccg ctcgattttg
     1321 ttttctaccg tggtctgaac gtcagcgaag cttctgtatt ggttacgcgc gcttccgatc
     1381 acaatccgct actcgttgaa ttcagtcccg gcaagcctga taaataaggt atgtcaggtc
     1441 tgccacaggg cagaccaacg tttggcgctg tccttttata ctcaaacaac aaaaggacag
     1501 cgcaatgaca acacaatccc accatgacca cgtagaaaag cagtttagct ctcaagcctg
     1561 cgaatatctg accagtaccg tacatgcatc cggacgagat ttacagcgcc tggcggtgcg
     1621 tctggctgat tatcctggtg caagtgcact tgatatgggc tgcggagcag ggcatgccag
     1681 ctttgttgct gcgcaaaacg tgagcgcggt ggtggcgtat gacttatctg cccaaatgct
     1741 ggatgtcgtg gcacaagctg ccgaagcccg gcaactgaaa aatatcacca cccgccaggg
     1801 atatgccgaa agtctgccat ttgccgataa cgcatttgat attgttatca gccgttattc
     1861 tgcccatcac tggcatgatg ttggtgcagc actgcgtgaa gtgaaccgga tattgaaacc
     1921 tggcggtagg atgattgtga tggacgtaat gtctccgggt catccagtgc gcgacatctg
     1981 gttacagacg gtagaagcat tacgcgatac ctctcacgta cgaaactacg ccagcggtga
     2041 gtggttgagg ttaatcaatg aagccaatct gatagttgat aatttaatta cagataagtt
     2101 accgctggaa ttttgttcat gggtcgcgag aatgcgtacg ccagaagcgt tagtagacgc
     2161 tattcgcatt taccaacaga gcgcatcgac agaggtgaga acgtattttg ccttgcagaa
     2221 tgacggctct ttcaccagtg atatcatcat ggtagaagca cataaagcgg cataaataaa
     2281 aaaggcaccg ggggaatcgg taccttttta ttatctggtt tgtcaggaat ctggcatgtt
     2341 gttgtttttc acaaacaacg tcagcttgtc gcctggttgc agattcgcag tgtcgctgtt
     2401 ccagcgcatc acatctttga tgttcacgcc gtggcgttta gcaatgcttg aaagcgaatc
     2461 gcctttgcgc acacgatacg taatgctatc gctgttgttt gccagtcgct gtgcaccaat
     2521 cgtcaaactt tggcctggct tcagcttaga tccgcgcagt ttgttccact gctgcaaatc
     2581 tttggtgctt acgccgagac gtgaagcgat gcttgaaagc gtgtcgccag agcgtacggt
     2641 gtaaacacgg ctgttaagcg gcgtattgtt ggcaaccagc gtcgactgta cagcagcaat
     2701 ttcgcctgaa gcgagagatt cacgcagttg atctgcatgc ttctttggca ccatcacgta
     2761 ctgcggccca cttgcgccca gcgtggagcc tttcacgcca gcgttgaatg tcttcagctt
     2821 gctgacggaa atccccgcca tatctgcaac cttcgccatt tcaaccgggc tactcaggtg
     2881 cacacgcgcc agagcacggc tttcatcggt cgttggcaga cgtacgccat aacgcttgct
     2941 gtttttgaga atatcactca atgccagcat tttaggcacg tactgcttcg tttcctgcgg
     3001 caacggtaac gaccagaagt ccgtggattt cccacgcgct ttgttcgttt taattgcctt
     3061 catgacccga ccttcgccgc tgttataagc cgctacggtc agaagccagt cgtcgtcaaa
     3121 cattttgttc agacgctgca tcatattcag cgcggcagtt gttgaagcaa caacatcgcg
     3181 acgcgcgtca taattgcggg tctgtttcaa accataattg cgccccgtgc tcggaatgat
     3241 ctgccagatg cctgcggcat tggcgccaga cgttgcgtga ggatcaaaag cgctctccac
     3301 tatgggtagt agtaccagtt ccataggcat gttacgtttt ttaacttgcc ctgctatcca
     3361 gtacatatac ggctctgccc gtaaagttac atcgtggaga tagctcttat tgcgtaaata
     3421 tttctgtttc tgttcgcgaa tccggtcatt ttccggaatt cccatcttta gctcgtcgcc
     3481 aatgaaagcc cacaagtcac catctggcgc gatagacgtc ccatcgtcca tccatcgtgc
     3541 ctgacttgta aactttgctg cttccccttg accagctgca gaaaggctct gtgcgtgctg
     3601 ttgaacgttg ccggtactct ggcaacccac gagcaggaca gaggcgagta atatcgcttt
     3661 tgccttcatg tgtgtgtcaa tagttgctta aaagacgacc gatcataacg gcgaacggag
     3721 ccgatgacaa gaaagtttta tcagaaccta tctttctttg accttaacca tgcaaaacgc
     3781 tcttcaggtt gttgcaataa tgtttcttca ttaattccat taattaaatc aatatcttcc
     3841 gttcttaaaa aaacattaat ttgccgctca tttttcagaa ttacgggtag tgttatttga
     3901 ttttttgccc gtaactcctt aactttacga taataatcat ttatggacaa atcgtgcgga
     3961 aaaatactca aagcaaactt catatttgat aacgtatatt catgagcaca acataccaat
     4021 gtatcgtcag gtaacgcact tagcttttta agtgattgat acatttgtaa agctgtccct
     4081 tcaaacaacc gaccacaccc accagaaaac agcgtgtcgc cgcaaaatag ataaggttta
     4141 ctgaagtaac agatatgtcc taaagtgtga cccggtgtgg taattacact aaattcatgc
     4201 cctaaaacga aggcagtttc gccatctttg actacctgtg ttgttccctt atcttgtgtc
     4261 tcttgtggac catacactac aatttgcgga aacttttcca ccagttcttt tacgccgcca
     4321 acgtgatcgt ggtgatggtg ggttagaaat atggcctccg gttgccagtt attggcggta
     4381 atggcgttta atactggctc tgcgtctccg ggatcgacaa tcaggcagcg acctgcctca
     4441 tcattcaaaa cccagatgta attgtcatca aaggcgggaa tactgttaag attcatagat
     4501 tacctctcag tgtaaaacgg aaggttgtga tgaaaccggc aagagtccct caaactgtcg
     4561 tggctcctga ttgctggggc gatttgccct ggggagagct ttatcgcaag gcgctggagc
     4621 gccagctcaa cccgtggttc actaaaatgt atggttttca tctgcttaag attggcaatt
     4681 taagcgcaga aatcaattgc gaagcgtgcg cggtttctca tcaagtgaat gtttctgcgc
     4741 aaggaatgcc cgtccaggtg caggcggatc cacttcatct tccttttgcc gataaatccg
     4801 ttgatatttg tctactggca cataccttgc cgtggtgcac cgatccgcat cgtttattac
     4861 gtgaagccga tcgggtactg attgatgatg gctggctggt gattagtggc ttcaacccca
     4921 tcagtttaat gggattacgc aaacttgtgc cggtattgcg caaaacctcg ccctataata
     4981 gccggatgtt tactctgatg cggcaactgg actggctctc tttgttgaat tttgaagtgc
     5041 tgcacgccag ccgtttccac gttctcccgt ggaacaaaca cggaggaaaa ctattgaatg
     5101 cgcatattcc tgcgcttggt tgcttacaac ttattgttgc ccggaaacgg actattcctt
     5161 taacgctaaa tccgatgaaa cagagtaaaa acaagccacg aattcgccag gcggttggag
     5221 ccacccggca atgtcgtaaa ccacaggctt aaacttcgac ttggtagccc gtatcttcca
     5281 gtgtgggatt catcgccgcg gcacgagcca gttcatcaca gcgttcgttt tccgggtgtc
     5341 cggcatggcc tttaacccat tcccatttga tttgatgctg ccccaatgca gcatcaagac
     5401 gttgccagag atcgacattt tttactggtt ttttgtctgc tgttttccag ccacgttttt
     5461 tccagttatg gatccactgg gtgataccct ggcggacata ctggctgtcg gtactcaaaa
     5521 tgacttcgca atgttctttt aacgcctcca gcgcgacaat agcggccatc aactccatac
     5581 ggttgttggt ggtgcgggtg tagcctgcgc taaaggtttt ctcgcgtccg cgatagcgta
     5641 aaatagcgcc gtaaccccca ggtccaggat tacccagaca cgaaccatcg gtgaaaattt
     5701 ctacctgttt aagcatctct ggtagacttc ctgtaattga atcgaactgt aaaacgacaa
     5761 gtctgacata aatgaccgct atgagcactg caattacacg ccagatcgtt ctcgataccg
     5821 aaaccaccgg tatgaaccag attggtgcac actatgaagg ccacaagatc attgagattg
     5881 gtgctgttga agtagtgaac cgtcgcctga cgggcaataa cttccatgtt tatctcaaac
     5941 ccgatcggct ggtggatccg gaagcctttg gcgtacatgg tattgctgat gaatttttgc
     6001 tcgataagcc cacgtttgcc gaagtagccg atgagtttat ggactatatc cgcggcgcgg
     6061 agttggtgat ccataacgca gcgttcgata taggctttat ggactacgag ttttcgttgc
     6121 ttaagcgcga tattccgaag accaatacct tctgtaaggt caccgatagc cttgcggtgg
     6181 cgaggaaaat gtttcccggt aaacgcaaca gcctcgatgc gctatgtgcc cgctacgaaa
     6241 tagataacag taaacgaacg ctgcacgggg cattactcga tgcccagatc cttgcggaag
     6301 tttatctggc gatgaccggt ggtcaaacgt cgatggcttt tgcgatggaa ggagagacac
     6361 agcagcaaca aggtgaagca acaattcagc gcattgtacg tcaggcaagt aagttacgcg
     6421 ttgtttttgc gacagatgaa gagctggcag cacatgaagc ccgtctcgat ctggtgcaga
     6481 aaaaaggcgg aagctgcctc tggcgggcat aaatacatgt aaaaggcgct aaaaatagcg
     6541 acttgggcga tttttgcagc aaacgattca aaagatgaga aaaatcgttg acgaaggtcg
     6601 aggcaatccg taatattcgc ctcgttccca acggaacaca acgcggagcg gtagttcagt
     6661 cggttagaat acctgcctgt cacgcagggg gtcgcgggtt cgagtcccgt ccgttccgcc
     6721 aatattcaga aagcccgaat cagtaatgat tcgggctttc gtcgttttgg tgaagtgaga
     6781 atttttcact ccgaagtaaa acggatatga tttaaaaaat ggccagacat tataaattct
     6841 ggccacttct gttactccag cgcatcccat ttctccagca gttctactgg aatttcattt
     6901 attacctccg agacgcgttt tccgaccagg cgttctgcct ggcgcagcaa aggaatggtc
     6961 gggctgctcg gttcgctgtg ctcaaaccag cgacggatga cacgcaggcg ttccagcgcg
     7021 tcgtttcggt cgcggatctg ccggggttct gcgccggtca tcggtgatat ttcctccgcg
     7081 atatgcaccg gtagcgcaat ctgctctgcc tgcatctgtt cagtatgaac gatggtggcg
     7141 ttttccggct gctcagcctg tggttgtggc aggatctccg gcgtggtgct ctgcactgct
     7201 cccggtaaca gggccagcag ttgcgtcaga cgggagaagt ccggtgcctg gtcattcagc
     7261 gtttcacgcg catggcgttg cagccgctca gtggtctctg ctgcctggcg aaaggcatcc
     7321 agcggcagtg tgccgcgcgc ttccaggtct gccagttgct ggcgtaccga ttctggtgcc
     7381 agcgcatccg ccgggcgtaa tgcagatagc gaacgctcca catcccgcac ctgaaggcgc
     7441 atggcagcat tatttgacag tgtaatgcca cgaatatccg ccattacacc ttcgtggtcc
     7501 agcaacgccg ccagcgcgtt actgcgtgcc accgcagcat cttcggcggt gatatcttca
     7561 gtcgcgagca gttgaggatg gatggcgtcc gggtacgtgg agcacaacgt ttccagcagc
     7621 gtgagcgcct ccgacagtcc cctggcaccg gcctgctgga tacggcagcg cagcaggaca
     7681 accagaatgc ggatatcttt gctgcgcatc agcagacgat gggcgtcccg ctcaagctcc
     7741 gcccagttaa tgttttccgg cgtactgaca aaatcgccat attgcgcttc ttcacgcggt
     7801 gcggcgcggg tgaacagcag caggtattcc ggatcgtact ccagatccgg gccgcagggg
     7861 cattccgccg aaattgggtc agacaggaat atttccattt aattatcctc agtgcgccag
     7921 cgtggtggcc agaccgtccg gattgctgga ctcacgcacc agatgggccg atgccgccag
     7981 acgagcgtga tctggcaacg ggctgtcagc gctctcccgc aaatcctgcc cgctgaggct
     8041 ggcgacataa aaggcaaagc ggtcatgttg cggtttatcc agaccgcccc cagcggactg
     8101 ggcgctgcgc catgcctggt aaaactgcgt cagccagcgg tgatgaaaaa gatcagaaaa
     8161 acgggtcagc gtcggctttc agtccggtta cgcgccagtt cggtgtagtg cagcggcagt
     8221 ggaccgttcg gcccccacag cacgaggctg tacagactga tgtgcaggcg cccttcctgc
     8281 cagcccacac tggcgatttc tcgtggcgca aaggtcagcg cgggcgtttg tccgaggcga
     8341 aatttttcca tccgtggttg ccaggtgttg cccgccggaa cggtacacaa ctgcacatcc
     8401 acgcggcgca tcaggttcag aaaaccgtaa cgccaggggg gggcctgcgc ctgagtgaaa
     8461 tcgttgctca tacgcttccc cgctgtcccg ccctgacagg ccaggtcata attttgccgc
     8521 gctgcatgct gtgcagcgtc atctgcgaga aggtattaat ggaaacatgg cgggcgatat
     8581 agtgttccag caccaggccg aacagatacg gactgatgcc ggaaaaacct tcttcatcca
     8641 ccgtcagttc acaactgacg ccacgcccat agaccagtag tccggaaccg ggcagacggc
     8701 gggtgaccgg ggtcgtttta caaccaatca ggctgcgcac ctggcgcgat tgcggactgt
     8761 catgagcagg aataaacagg ttcagcagat ctcgcaacgc ctggccgccg gtacggtgat
     8821 ccagatcggc cagcggcaga taattaaaag ataactggcg gatcaaccgc catgccatct
     8881 cacgttcagc catcgggggc tgcggcgagc gtggcgggcg gataagccct acgcccgcca
     8941 ccgggatggc tgcatccacg gttagatcgt cccggccatt gcgggcaata agacagggca
     9001 gatcgcggtt agtgaccatg gccgtgatgg tgatgtggcg cagattttcc gggtacggcg
     9061 cttcatactg gtcaaccagc gacaggaaca cttccgagcc cgtatagggg gtacgggtgc
     9121 cgtaacggcg ggcgttttct gataagcggc ggggctcccg gcgcagggaa aaataacgtc
     9181 cgtgattgcc ttcatcatta ttgcgggtgt ggtagagcgg gcgaaaggcc atcttccgtg
     9241 tggtgtcggt ttccagccct tccacttcct ggaccgagaa cacctcataa tccagcggat
     9301 gggttctgtc caccaccaga tgctgttccg tcgtgctgtg cgtgacatcg atccgggcgg
     9361 tgacgcgggg aaacaggttg ataaccgggg tacagaacag gctgaactgt gccgcatttg
     9421 tctggtggat cagccagtcc ggcggcaggc ggttcagcag gataacaatc tccgccacgc
     9481 caccgtcaat tttctgtaat ccggcagaca ggcctgtcgg ggtgaaaaaa tagaagcgtt
     9541 ccggacaggc aaaatattca tgcagcaggt tatgaccgtg aaacacgttc cacgccagcg
     9601 gcagcagccc ctgatccggc tccaggcctt cgtacataac gggctgttgc agattcacat
     9661 ccagcgcgcc gtcaaaatgc cccggaatgc cagccagggg cgcgatggcg ctggtatgta
     9721 gaagttctaa caggtgtgag gccgtacgct cctcgccgca gagataaaac ggcagcctgt
     9781 ccagtccggc aagctgactg aacgttagtt caccaaaggt gcgcagggta atacgcagcg
     9841 ccccggcgac gtgaatgttg gccggcaggt agcggtgcag ggcaggcata tccggtggcg
     9901 cggcggtcag tcgcgcctct tcgatggcga gcggccacag cgttacatcc tggctgctgc
     9961 gaaactggca ggcagtgctt tcgccttccg gaatggcgga gaaaaaggct gtgtcgcgcg
    10021 ggacggtaaa tcctttcgcc agatcgcctt cttccgtatc gggatgcaac tgcgccacgc
    10081 tcattgaggg cgtgggtgtg acgtaattgg ggctgaccac ttccagcagg cgctgggtga
    10141 aacgggggaa ttcagcgtca atttttagct gcgtgcgggc ggtcaggaag ctgaaggcct
    10201 cgaccattcg ctctacatac ggatcggcaa tatccgttcc ctgcatgccc agccgggcgg
    10261 cgactttggg atgacgggcg gcaaattcgg ctcccgtttc ccgcagatag ctcagttcgc
    10321 ggttgtagta ttccagtagc cgtggatcca tcggtctctc tctgaattaa aaaaacgtaa
    10381 tgcgactctg ctccatatcc agcgcgctac gtacccggaa tgcagtcgga tacggctgcg
    10441 tgaggatctc gccgcgaatt tcaaactgta atgtgttatg gccactctgg cggcgctcat
    10501 cctgaagtgg gatgatttgc agagtggacg ctttcagccg gggttcaaac cgggtgatgg
    10561 ttcgcaaaat ggccctgcgg atatcgtccc acttgtgctc atacagaaag ctgcccgcca
    10621 gtggcggcag gccgtagtta agtacggacg ctgccgcgta cggataacgt gcagcatcaa
    10681 tatcatcttc atggctgacg gtgttgagca gaaatgacaa atcccggcgg atgatctcct
    10741 taatctgaac cggactgacg ctgataccgt ggtcgcgttt ctggtttggg gcgtcgtcac
    10801 acaggcggtc aaacagcgtg ggcagcaggt gattgcctgg acgctggcgg gaagcgctca
    10861 cgcgtcgctt ccttcagcgg tgttaaaggt acaggtggac aaatccagca ggctgatatc
    10921 gccgtgaccg gtgagccaca ccctctgacc cagcgcctgt accagtgtct ctcccggacc
    10981 gtcctgccag accgtttccc ggcacagacg cagggagtcg gcagcctttt cagagccgct
    11041 gtagcgggga aacagctagg cactgtgtgt atcgccattg ttcagggtga cgttgacggg
    11101 tttccagatc aaatcgatca gatgcgcagg ccgcggcgat ttcagcgaac agatttgtga
    11161 aaacggcagc cagatatatg ctccgcccgc gatcagttcg agtaccggac cggtacggga
    11221 gtcgctgtcg ctaatccagt caaatgcgcc gccattccac tgcccggaag tgtctgttat
    11281 ggcctcaagc gcgcggctgc ggtgagcatc agcttcctgc gcttcaccac gggcgttaca
    11341 ggcgagggcg gtcagcagat cctccatcca ggcgggcggc gggagaataa caccgggccg
    11401 ctgttcgccc tgaaagcagg cgtggcggta tatctcacag cggatcagct cgccaaatac
    11461 ctgcgcttcg cgagtataat ttgcgtccat ccgggcgcat agctggatct gttgcagtgc
    11521 ccgcgaccag tcgcctgcca cgcacaataa ctgaaacagg ctgtggcggc gggaggcctg
    11581 cgctgggtgc atcctgacct gttgctctgc catctgaatg ccctccacca cggaataatc
    11641 ccgcagcagc gccgacaagg taatcggtag ctcgttattg tttgtcatga tatatctctc
    11701 catgttgtgc cgggtttatc cggtaatggc tgtcaatgcc aatgatgcga tgctctttcc
    11761 gtgtcagatc tggcagtacg gtgttatgcg ctgtttccgg ggcgtattcc ggggaaagta
    11821 aatgcaggat atcaggggga gtcttttcga caggccagat agtttcatct tcgccagtgg
    11881 catccagtac attgagaata gcgtcaatgc ttaacccacc agtgaccata tcctgcaatg
    11941 tctcggcttc ggtgaccagg tcacgcaatg aatccagatc ctgcgaggag ggctcttcgt
    12001 taaaaggaaa aggatcagca tggcgattaa gatcttgttc gggagataac aacgcctcgc
    12061 ggtattcctg ataaagtcgg gtcagggcat ttcctttttc cgcaccttct ccggtggagg
    12121 aggctccatg aacaggaatg atatcgaatg gattttgtga tgtcagatgc tgctgcctga
    12181 accaatccag atccagatat tcggttattg cttccgacgt gatggcagtc tgcgatatcg
    12241 tttttactgt gctcaggttc ggaga
//
LOCUS       DAJTYM010000105        12249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTYM010000105 DAJTYM010000000
VERSION     DAJTYM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..652
                     /locus_tag="OO575_003209"
     CDS             <1..652
                     /locus_tag="OO575_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR6666064.1"
                     /translation="ARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHP
                     QAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGG
                     EQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFR
                     LYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
     gene            653..2818
                     /locus_tag="OO575_003210"
     CDS             653..2818
                     /locus_tag="OO575_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6666065.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            2832..3743
                     /locus_tag="OO575_003211"
     CDS             2832..3743
                     /locus_tag="OO575_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR6666066.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            3743..5038
                     /locus_tag="OO575_003212"
     CDS             3743..5038
                     /locus_tag="OO575_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR6666067.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5083..5313
                     /locus_tag="OO575_003213"
     CDS             5083..5313
                     /locus_tag="OO575_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666068.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5291..5791
                     /locus_tag="OO575_003214"
     CDS             5291..5791
                     /locus_tag="OO575_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6666069.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            5791..7209
                     /locus_tag="OO575_003215"
     CDS             5791..7209
                     /locus_tag="OO575_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR6666070.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7209..8057
                     /locus_tag="OO575_003216"
     CDS             7209..8057
                     /locus_tag="OO575_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666071.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8057..8512
                     /locus_tag="OO575_003217"
     CDS             8057..8512
                     /locus_tag="OO575_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6666072.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8515..9207
                     /locus_tag="OO575_003218"
     CDS             8515..9207
                     /locus_tag="OO575_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6666073.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9217..10548
                     /locus_tag="OO575_003219"
     CDS             9217..10548
                     /locus_tag="OO575_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6666074.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10549..>12249
                     /locus_tag="OO575_003220"
     CDS             10549..>12249
                     /locus_tag="OO575_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6666075.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     D"
BASE COUNT         3295 a         3025 c         3238 g         2691 t
ORIGIN      
        1 tgctcgcggt attcctacga tgggtagtgg tcgaatcttc cagataccgg aagagacgat
       61 taagtgtcag ccgttcgagt gtcctgatca cttctacgta attggcggga tggatttcgg
      121 atgggatcac ccacaggcgc aagttcagct ttggtgggat aaggaggcag acacaatcta
      181 cgtttcacgc gtgtggaagg cgaaagaaaa aacagccgtt caggcgtggg gagccgttaa
      241 atcatgggcg cataaagtgc caaccgcatg gcctcatgat ggaaaccagc acgagaaggg
      301 cggcggtgag cagctcaaag ggcagtatgc agacgctgga tttatgatgt tgcaggagca
      361 tgcgacatgg cctgatggcg gtaacgctgt tgagccaggc atcactgaat tgcgcgacat
      421 gatgctcgat ggtcgcttca aagtattcaa tacctgtgag ccattctttg aggagttccg
      481 cctctatcac cgtgatgaaa acgggaaaat cgtcaagctt aacgacgacg tgctatccgc
      541 cgttcgctat gcatacatga tgcgccgctt cgcaaaaatg atgcgcgaca tcaaaaaacc
      601 aaaagagaaa aagataccag ccccaatcag gcccatcgca cggagaactt aaatggccga
      661 cgaaaacaga ctcaattcca ttctgtgtaa gtttgacgca gactggatgg cgagcgatga
      721 agccagaacc gaggcgacaa atgacctgta ttttagccga gtgtcgcaat gggatgactg
      781 gctatcaaac tacactaccc tgcaatatcg cggacaattc gatgttgttc gcccggaggt
      841 caggaaactg gtcgcagaga tgcgccggaa ccctgtcgac gttctcttcc gacccaaaga
      901 cggcgctaat cctgatgcag ccgatgtatt gatggggatg tatcgtactg atatgcgcca
      961 taacacggca aagattgccg ttaacgttgg cgttcgtgag cagatagagt caggcgttgg
     1021 tgcatggcgt ctggtcacgc agtacgaaga caacgatcca acaagcaaca atcaggtaat
     1081 ccgacgcctg ccaatccatg aagcctgctc acacgtcata tgggacgcca acagcaagca
     1141 gatggataag agcgacgcta agcactgcac ggtgattaac gccttgtcgc gcaatggctg
     1201 gaaagagttc gcagaggatt acggtattga cccggatacc ctgccatctt tccagaatcc
     1261 taacgataca tggctattcc cgtgggtgtc gaatgatgtc gtctacgtcg ctgagtatta
     1321 cgaggtagaa gaggagaagg agaaggtctt catctaccgc gacccgctga caggtgagcc
     1381 ggtcagctat taccagcagg atatcaaaga cgtcatcgac gacctggcta atcgtggatt
     1441 cattaaggta gcagagcgca aggtgaagcg tcggcgtgtg tataagtcga tcatcacctg
     1501 tacgcagata ctgaaagacc gcgagaagat agccggagag catattccaa tcgttccagt
     1561 gtatggcgaa tggtcattcg ctggtgacaa ggagtgctac gagggtgtgg taaggctgac
     1621 gaaagacggt caacgccttc gtaacatgat tatgtcgttc aacgccgata ttgttgctcg
     1681 gtcaccgaag aagaaaccga ccttcttccc tgagcaaatc gaaggctacg aatacatgta
     1741 cggcggaaat gatgactatc cgtactatct gcagaacagg actgatgaaa acggtaacga
     1801 cctgccgatt ggtccaatct cctacatgga aaaccctgaa gtgccgcaag ccaacgctta
     1861 catgcttgag gctgccacca acgcagtgaa agaagtggct agtcttggcg tggatgcgca
     1921 ggcggcaaat ggtcaggtcg cttttgatac cgtcaatcaa ctgaacatgc gggcagacct
     1981 tgagacatac gtgtttcagg ataacctggc taccgcaatg cgacgtgatg gcgagattta
     2041 tgcctcaatg gtcaacgata tttatgacgt tcctcgtcat gtaacgctga cacttgaaga
     2101 tggaagcgag aaagacgttc aactctatgc gcaagttgtc gattaccagt ccggcaatgt
     2161 ggtcacactc aacgacattc gcggtcgata tgagtgctat acagacgttg ggccatcctt
     2221 ccagagtatg aaggaacaga accgcgcaga gattcaggag ttgctcacca aggttccgca
     2281 aggtactcca gagttccaga tgctgatgct gcaatacttc acgctgcttg acggtaaagg
     2341 cgtcgagatg atgcgagagt acgcgaacaa gcaactggtg atgatggggc tgaagaaacc
     2401 agaaacacct gaagagatgg agatggtgca gcaggcacaa caacagccgc agcagccatc
     2461 agcagagcaa attcaggcgc agggtatcct tctgcaaggt caggctgaat tgctcaaggc
     2521 agagaaccaa caggcgcaga ttcaggttga agctgccaag gttgaagccc aaaaccaact
     2581 caacgccgcg aagattgcag aaatattcaa caatatggac ctcgacaagc aggcagaact
     2641 gcgtgagtac ctcaggctcg taggtcaatt ccagcaacag cgcagcaaag atgctcgtgc
     2701 taacgctgag ctgcttctta aagatgcaga ccagactcat tcacaacgca tggatttcgc
     2761 gaatcttatg cgtcaagttc aaatcccctc cggcggagta gccgagacac ctcaataaga
     2821 gagagttaat catggaccaa accaccgaca ttcaggcttc tgaagaatta accctgcccg
     2881 gcaatcatgc agcggcatct gctgatggct tagttgtcga taatgccaac gacagcgcag
     2941 gtcaggaaga aggctttgag attgtccgga aagacgatga gaaaccaaaa caagacccgg
     3001 caactaatgc tgaatttgcc cgtcgccgca tcgaacgcaa acgccagcgt gagcttgagc
     3061 agcagatgga agcggttaag cgtggagagt tgccggagca cctgcgggtg aaccctgagt
     3121 taccaaaaca accagaccct aacgattatc tttccgaaga tgcactggct aagtacgact
     3181 atgaccagag ccgcgcactg gctgccttcc agcaggcaaa cagtgaatgg cagatcaagg
     3241 ctatggacgc acgaagccag gctgtcgccg agcagggtcg caaaactcag gagttcaccc
     3301 agcaatcagc gcaatacgtc gaggcggccc gtaagcacta cgaggcagcg gaaaagctca
     3361 atatccctga ctatcaggag aaagaggatg cattcatgca actggtgccg ccagcagtcg
     3421 gtgccgacat catgcgcctc ttcccggaga aatccgccgc tctcatgtat caccttggtg
     3481 ctaatcctga gaaaacacgc cagttgctgg cgatggacgg gcaatccgcg ctgattgaac
     3541 tcactcgact gtcagaacgt ttaactctca agcctcgagc caagcctgtt tcagaagccc
     3601 cgttacctga tgaacccatt cagggacatg ctgttgctgc aaatatctct gcgattgaaa
     3661 agcagatgga ggcggcagca aacaaagggg atgtagagac gtaccgcaag ctcaaggcgc
     3721 aactgaataa aggaattcga taatggcatt aaatgaaggt caactggtca cgtatgctct
     3781 ggatgaaatc atcggaaccg tccagaacct gacgccaatg gcgtcaaaag tgacaaaata
     3841 cccccctccg gcagaatcca tgcagcgttc aagcaacacc gtgtggatgc ctgttgagca
     3901 ggaagcgcca acccagactg gctgggattt aactggcaac gcaaccggga ttctggaact
     3961 ctccgtgaag tgcaacatgg gcgatccgga taacgatttc ttcgagcttc gtgcagatga
     4021 cctgcgtgat gagcgttctt accgtcgccg catccaggca tccgccaaaa aactggcgaa
     4081 taacattgag tcagcgattg ccaaacaggc aactgaaatg ggctcgcttg ttgttcacga
     4141 tacccgcgca attggtccat cgactggcct gcctggctgg gattttgtgt ctgatgcaga
     4201 gcgactgatg ttctcccgtg agctaaaccg cgacatgggc atcagttact tcctgaaccc
     4261 tgacgattac cgcaaagcag gccgcaacct ggtagatggt gacatcttcg ggcgcgttcc
     4321 tgaagaagca tatcgaaacg gtactattca gcgtcagatt gctggctttg atgaaattct
     4381 tcgctcaccg aaacttccgg cagttaccaa gtcaaccgcg actggtgtaa ctgtttctgg
     4441 tgcgcagaag tttaagccgc aggcatacac ccttgatacc gatggtaaca aagagaacgt
     4501 cgacaaccgt gttgcaacgg tgaccgtatc ctccaccacc ggatttaagc gcggcgacaa
     4561 aatcagcttc attggtgtga agttcctgtc tcagatggcg aagaatgtgc tgactgatga
     4621 cgcgactttc tcaatcaccc gtgtgatcga tggtactcac atcgaaatca cgccgaagcc
     4681 gattgcgctg gatgacgcgt cactgacaaa agaagagaag gcttacgcta acgtaaacac
     4741 ctctcttgct gataccactc cggtaaacgt tctgaacgtg gcaacaacca ccgctaacgt
     4801 gttctgggct gatgactcaa tccgcctgct gtctcagccg atcccggtaa cccatgaact
     4861 gtttgctggc atgaaaacgt cttccttcag cattccaggt attggtgtta acggcatctt
     4921 cgcaacgcag ggtgatatca acactctgtc tggtaagtgc cgtattgctg tgtggtattc
     4981 agcatgtgct gtacgaccag aggcaattgg tgttggtctg cctaaccaga ccgcgtgata
     5041 accagaggga gcttcggctc ccgtttttat ctggagacaa gcatgacaca catgatcttt
     5101 cgtcatggcg acatgaagaa gtggaaaggc gttggctacg actttgaaat cgtgaaagcc
     5161 gaagagcttc aggaatatct ggatgctggc tggttttcac atcctgatga ccttttgaag
     5221 gatgttgcag agccagagcc agaagaaaaa cagcgtaaaa agcctggtcg aaaacctaag
     5281 gcggcagcag atgaacctga caacgaaggg tgatttagtt cttgcggcat tacgtaagct
     5341 cggtgttgca tcaaatgcca cgttaaccga tgtcgaaccg cagtctatgg aagacggcgt
     5401 caacgacctt gaaatgatga tggctgaatg gcttggcggt gatgcgtcac ctggtatcaa
     5461 cgttggctac atttttgctg atgcagatgt tgctccggat ccgggcgatg agcacggttt
     5521 atcaaataac gctatcaatg ccgtcatttt caaccttgcc tgccgaattg ctccagatta
     5581 tgcgctggaa gcgtctgcaa aacttataac cactgccaga tacgggaaag agcgactcgt
     5641 caaactgtct gcaatggaca gggcaaaagc agctaaatgt aagtccggtt atccaaaccg
     5701 tatgcctgtt ggtagtggta accagttggc gaagtggaat ggttggaatt acttccaccg
     5761 aaaggaacct tgcgataacg ggagcgaata atgcctattc agcaacttcc gcttatgaaa
     5821 ggtgtcggca aagacttccg aaacgccgac tatatcgact atctgccagt gaatatgctg
     5881 gctacaccca aagaaatcct gaacagcagc ggatatcttc gctcattccc gggcattgcc
     5941 aaacgttctg acgtgaacgg tgtatctcgc ggcgtcgagt acaacatggc gcagaatgct
     6001 gtttatcgcg tgtgtggcgg caagctgtac aaaggagaaa gtgaagtcgg tgatgttgcc
     6061 ggaagtggtc gtgtatcaat ggcgcatggt cggacatcac aggcggtagg cgttaatggt
     6121 caactggtcg agtatcgcta tgatggcacg gtcaaaaccg tctcaaactg gcctacagac
     6181 agtggattca ctcagtatga gttaggttca gttcgcgaca ttacgcgctt acgtgggcgt
     6241 tatgcgtggt caaaagacgg aactgattca tggtttatca ctgaccttga agacgaatct
     6301 catcctgacc gttacagcgc acaatatcgt gccgagtctc agcctgacgg catcatcggt
     6361 atcgggacat ggcgagactt catcgtctgc tttggttcat cgactattga atatttctcc
     6421 ctgactggcg caaccaccgt tggtgccgct ttgtatgttg cacaaccatc gctgatggtg
     6481 cagaaaggca ttgccggaac ttactgcaaa acgccattcg ctgattcgta tgcgtttatc
     6541 agcaatccgg caacgggtgc gccgtctgtg tatatcatcg actccggtca ggtgtcacca
     6601 attgccagcg cgagcattga gaaaatcctc cgctcctaca ctgctgatga actggctgat
     6661 ggtgtgatgg aatcgctgcg atttgatgct catgagttgc tgattatcca ccttccgcgt
     6721 cacgtcctcg tgtacgacgc atcttcaatc gccaatggcc cacaatggtg tgtgctgaaa
     6781 acaggtctgt atgacgatgt gtaccgcgct atcgacttcg tttacgaagg caatcagata
     6841 acgtgcggcg ataagctgga atctgttacc gggaaactgc aattcgatat cagcagccag
     6901 tacgacaagc aacaggaaca cctgctgttt actccactct tcaaagcgga taacgccaga
     6961 gtgttcgacc ttgaggttga atcgtcaact ggcgttgcgc agtacgccga ccgccttttt
     7021 ctctctgcaa ccactgactg catcaattac gggcgtgagc agatgattga gcagaatgaa
     7081 ccgttcgttt acgacaagcg tgttttgtgg aagcgagtag ggcgcatcag gaaaaatgtc
     7141 ggcttcaaat tgcgcgttat cacgaagtca cctgtcactc tgtctggcgc tcagataagg
     7201 attgagtaat ggctgattcg aatctcaatg tgccggtaat catccaagct acgcggctcg
     7261 atacatcagt ccttccacgc aatatcttct cgcagtcgta tctgctttac gttatcgcac
     7321 agggcactga tgttggtaac gtggctaaca aagccaacga ggccggacag ggcgcttatg
     7381 atgcacaggt caggaacgat gagcaggatg tgattctcgc tgaccatgag cagcgaattt
     7441 ctgctgcgga agcaacgctt gttaatcatg aggagcgaat cagacaggca gaatcaactc
     7501 ttcaggacca tgaaacacga atagctcaga atgaaagcga tatttcgtcg cttgacacaa
     7561 gagttcagtc gctggagtcg caggtttcag accatgaaac gcgcatcgat gctctggagt
     7621 atgcaaccac acgcaagaag tcagaggttg tttactctgg tgtatctgta accatcccga
     7681 cagcgccgac caaccttgtt agcctgctga aaacgctcac accgtcatcc ggctcgttgg
     7741 caccattctt cgacaccgtt aacaacaaga tggttgtgtt caacgagaac aaaaccctgc
     7801 tcttcaagtt gtcgattgtc ggcacgtggc ccagcggaac cgccaacagg tcaatgcagc
     7861 taaccttttc cggctctgtt cctgacacac tggtcagcag tcgtaatgcg gcgacaacaa
     7921 ccgacaacat cctgttagct acgttcttca gcgtggataa agacggcttt cttgccacaa
     7981 atggcagtac gttaaccatt cagtcgaatg gtgcggcgtt tactgccaca accatcaaga
     8041 taatcgcgga gcagtgatga ttacattcaa accaacgcga aacatcgacc tgatagaagc
     8101 agtcggaaat caccctgaca ttatcgccgg gagcaacaac ggtgatggat acgactacaa
     8161 acctgaatgc cgttactttg aggtgaacgt gcacggtcag tttggcggca ttgtttacta
     8221 tcaggagatt cagccgctga cattagattg ccacgccatg tacctgccag agattcgcgg
     8281 cttcagcaag gaaattgggc tggcgttctg gcgatacatt ctgactaaca ccaccgttca
     8341 gtgcgtaaca tcgttcgctg cacgcaaatt ccgacacggt cagatgtact gcgcaatgat
     8401 tggccttaag cgtgtaggaa ccatcaagaa atacttcaaa ggcgtggatg acgtgacttt
     8461 ttacagcgcc acacgcgaag aactaatcga cttcctgaat cacgggagat agccatgtta
     8521 tatgcattta agctgggcag aaaactgcgc ggcgaggaac cttattgccc tgaaaaaggc
     8581 gggaaaggtg gcagttctga taaaagcgca aagtatgcag cagaagccca gaagtatgct
     8641 gcagacctgc aaaatcagca gtggcagacg atcatgaaaa accttgctcc gttcacgcct
     8701 cttgcggagc agtatgttaa ccagcttcag aacctttcca gtttagaagg tcaggggcag
     8761 gcacttaatc agtattacaa ctctaagcag tataaagacc ttgcagggca ggcgcgttac
     8821 cagagtcttg ctgctgcgga ggcgacgggt ggacttggtt cgacagccac aagcaatcaa
     8881 ctggctacga tcgcgccgac actcggtcag tcttggttat caaatcagat gagcaattac
     8941 aacaatctgg caaacgttgg gcttggtgcg ctgcaaggtc aggcaaacgc cgggcagacg
     9001 tacgccaaca acatgagcag cattgcacag caaagcgcag ctcttgccgc tgctaatgcc
     9061 aataaaccat caagtcttca gactgcaatt agcggtggca cgtctggtgc gattgccggt
     9121 gcaggtcttg ccagcctttt gggaacatca acgccttggg gcgctggtat tggtgctggt
     9181 atcggattgc ttggctcgtt gttttaaggg ggaatcatgg ctacttggca aggaacaaac
     9241 ggcggattgt tggctggtat cggcggcgtc aactcaaacg ctccgagcgt aaatgacatc
     9301 ggcaatacgc ttcagcttat caggcagaac aatgatattg agcgttcagg cgctaacaat
     9361 gttgggctga ctgctttgca aggtctttca ggtattgcgg gtgtttttca gcaggaaaag
     9421 caggctcagc ggcagaaaga atttcagcag gcgtacgcta atgcttatgc gtctggtgat
     9481 cgcggtgctt tgcgtcagtt ggctactcaa tatccagacc agattgaatc cgttcgtaaa
     9541 ggcatgggat tcattgatga agagcagcgt aattctatcg gcaccttagc ggctggcgca
     9601 cgccttgcgt catcgtctcc agaagcaatg caatcatggc tgcaaaacaa cgccggtgag
     9661 ttagctcgtg ttggcgttaa tcctcatgac gtcgctcaga tgtaccaaca gaacccgcgg
     9721 cagttcggcg aatttgtcga tcacctgggg atgaacagtc tcgggcccga aaaatacttt
     9781 gacctacagg ataaaatgca gggtcgccag gttaccatgc gcggtcagga tctggattcg
     9841 caaaccgccg ctcggaatca ggcaatcaca atgcgcggac aagatatcca ggcgaattta
     9901 ggtcagcagc gcattaatct ggacgcagaa acaaaccgca ttaacaacga aaataagcgc
     9961 cttgaccgga tgctatcagc agaaactaac gacctgaagc gccaggaaat acagagccgc
    10021 atagcagcca acaaccagca gttgcagcag aagcagcaag cgctaaatga tggctacaaa
    10081 gacggcatca acaccctcac aaccagcatg ttcactctga acgatatcgt tagttctcct
    10141 tcacttaaga gcattacagg cttacgtgga gtaatcccca acgttccagg ctcacaggct
    10201 gcagacactc aggcacgact tgataccttt aaatcacaag catacctgac agcggttcag
    10261 gccatgcgag gcatgggcgc actttctgat gccgagggca aaaagctcga ccaggctgtt
    10321 ggttcgctgc agaactcgca gagcgaggag tcctttcgtc gcaacgctgg cgtcatcctg
    10381 aacacgctca accagaagcg taatgaggcg gttggtaagt acgttcagca aaacggtatc
    10441 aagcgagtgg aagcgcctca ggcttctata gattacctga agcagcaccc cgagctgtca
    10501 atcgacttca ttaatcgcta cggatatctt ccatctttgg ggcagtaaat ggctaattac
    10561 cgtgatttgt tagagcaggc tggcgcacgt tacggtgtgc cagaagggtt gatgactgca
    10621 ctgggtgcca aggagtcttc ttacaaccct gccgcagtaa gctccgccgg ggctgtagga
    10681 ttgactcagg tcatgcctgg gacatggcgt gatatgggtt ataccgatga gcaaatgcaa
    10741 aaccccgaat atcaggctga cgctggcgcg cgctatctgg caaagatgta ccagcagttt
    10801 ggtaactggc gtgactctct tcaggcttat cacgacggtc ccggcaacgt tatgaaggca
    10861 aagcgtggtg aatatacgcc aggacctgaa ggccgcggtt acgttgatga tcgctttgct
    10921 caatgggctg gtgacccggt gacagactca acagtcgaac agcgcgccac ctctgcaaag
    10981 gtacatcctc agcaagaccc taacaacccg tttgcacaac tggaagtaca gtcatccgaa
    11041 caagtatcgg catcaggtgt gcagtcagac ccaaataatc catttgctca gattgagcag
    11101 caggcagcca gtcagcagcc acctcaaccc gtaagttctg tcgcaccgaa acctgttcag
    11161 caacaaacag ttaatcaggc caataatgca ccaacacggg aagaaccatc attgatgcaa
    11221 caagctggcg attggctcac tggtggtcaa agtgcagggc aaattgcaga acaggctggt
    11281 cgtggtctgg taaacatacc atttgacgta ttgcagggtg gcgcaagtct gattaatgca
    11341 atcagtcagg ggcttggggg gccaaaagta ttggatgatg tttatcgtcc agtagacaga
    11401 ccgacagacc cctacgcgca agctggagag tcaataggcg gttatcttgt tcctggagca
    11461 ggtgtagctg gaaacatggt cattggttct ctcgctgacg cggcgaatca acggggtgat
    11521 tttgccgaaa atgccgctat taatgccgga cttagcattg ctacgcatgg cctgataaat
    11581 ggcgttaccc gtggtgttcg tggtgcatca aatataatta gtggcaataa aacatctgca
    11641 cagagagcga ccactgcgcc aacagaaaca tcaccattct ccggtgatgc cgctgcagca
    11701 acaaatcctg cggttcatgc cgcagaggca agagtagcac aaggtgtacc aatgacgcct
    11761 gcgacgaaga acccagagga agtcgttcgc acagtagcag cacaaaaaag gccaaatctc
    11821 gcttcatcgc ttgatgaact agatatcaat cctcaggctg aagttctgga gtctgctgaa
    11881 aggcttaatg ttgattcatt actcccttca cacttttccg ggaacgagca atacaaggca
    11941 gttgagcaag caatcaagtc ccgtgcgggt tctgctctac aggtgcagga aaatgaagca
    12001 atcaggcagc tagcacaggg cgcgggtgag ataattgatc gcgtttccgg tgcaaaagat
    12061 gctcttggta tgagcgacaa gtttattgat acggtcaatg gaagaatgtc tgcgctgatg
    12121 aaacgaagcg accagcttta tcgcaatgtt gaaaaggcga tgcctgcagg tgcaaaaatt
    12181 gatgcgccat caacaaggtc aatgctcaaa caggtggcag aagatcttgg cgggatgaaa
    12241 aaccttgac
//
LOCUS       DAJTYM010000106        12052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTYM010000106 DAJTYM010000000
VERSION     DAJTYM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO575_003221"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO575_003221"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO575_003222"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO575_003222"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6666076.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO575_003223"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO575_003223"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6666077.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO575_003224"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO575_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO575_003225"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO575_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6666078.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO575_003226"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO575_003226"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6666079.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO575_003227"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO575_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6666080.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO575_003228"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO575_003228"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR6666081.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO575_003229"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO575_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6666082.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO575_003230"
     CDS             9553..10332
                     /locus_tag="OO575_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6666083.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO575_003231"
     CDS             10322..11131
                     /locus_tag="OO575_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6666084.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO575_003232"
     CDS             11218..11652
                     /locus_tag="OO575_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6666085.1"
                     /translation="MLNIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12052
                     /locus_tag="OO575_003233"
     CDS             11649..>12052
                     /locus_tag="OO575_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6666086.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKG"
BASE COUNT         2879 a         3247 c         3073 g         2853 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaaatatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa gg
//
LOCUS       DAJTYM010000107        11846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTYM010000107 DAJTYM010000000
VERSION     DAJTYM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1293
                     /gene="maeA"
                     /locus_tag="OO575_003234"
     CDS             <1..1293
                     /gene="maeA"
                     /locus_tag="OO575_003234"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6666087.1"
                     /translation="DDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLY
                     TACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQR
                     WPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSE
                     KKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQT
                     KLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRP
                     IVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPG
                     IGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVG
                     KMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1427..2437
                     /gene="adhP"
                     /locus_tag="OO575_003235"
     CDS             1427..2437
                     /gene="adhP"
                     /locus_tag="OO575_003235"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6666088.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            2583..2867
                     /locus_tag="OO575_003236"
     CDS             2583..2867
                     /locus_tag="OO575_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6666089.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3095..3748)
                     /gene="fdnI"
                     /locus_tag="OO575_003237"
     CDS             complement(3095..3748)
                     /gene="fdnI"
                     /locus_tag="OO575_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6666090.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(3741..4625)
                     /gene="fdnH"
                     /locus_tag="OO575_003238"
     CDS             complement(3741..4625)
                     /gene="fdnH"
                     /locus_tag="OO575_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6666091.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4638..7685)
                     /gene="fdnG"
                     /locus_tag="OO575_003239"
     CDS             complement(4638..7685)
                     /gene="fdnG"
                     /locus_tag="OO575_003239"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7098..7100),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6666092.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            7917..8798
                     /gene="yddG"
                     /locus_tag="OO575_003240"
     CDS             7917..8798
                     /gene="yddG"
                     /locus_tag="OO575_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6666093.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9053..10153
                     /gene="ompC"
                     /locus_tag="OO575_003241"
     CDS             9053..10153
                     /gene="ompC"
                     /locus_tag="OO575_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6666094.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10317..10802
                     /locus_tag="OO575_003242"
     CDS             10317..10802
                     /locus_tag="OO575_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6666095.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            10912..>11846
                     /locus_tag="OO575_003243"
     CDS             10912..>11846
                     /locus_tag="OO575_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6666096.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFP"
BASE COUNT         3082 a         2945 c         2775 g         3044 t
ORIGIN      
        1 gacgatattc tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt
       61 gaacgtattc tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa
      121 ctgtcgctct ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg
      181 gatgtcggaa cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat
      241 ccgcgtatca ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa
      301 caacgctggc cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg
      361 ttacttaacc gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg
      421 gcggtaacag tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag
      481 aaaaaaatcg tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc
      541 gcccagaccc agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc
      601 gatcgctttg gcctgctgac ggacaagatg ccgaacctgc tgcctttcca gaccaaactg
      661 gtgcagaagc gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg
      721 gatgtggtgc gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg
      781 tttacggaag agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg
      841 ctgtctaacc cgacgtctcg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa
      901 ggtaacgcgc tggtcgccac tggcagcccg tttaatcctg tggtatggaa agataaaatc
      961 taccctatcg cccagtgtaa caacgccttt attttcccgg gcatcggcct gggtgttatt
     1021 gcttccggcg cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacgcttgct
     1081 cagtattcgc cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt
     1141 cagaaagtct cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg
     1201 gcggtcaaaa cgtctgctga agctttgcaa caagccattg acgataattt ctggcaagcc
     1261 gaataccgcg actaccgccg tacctccatc taagcctgca cccggtagtg aaggctaccg
     1321 ggctatttcc ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc
     1381 atttgcctca cctgctatgc agaacatcat ccgaaaagga ggaactatga agactgcagt
     1441 tgttacgaag gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg
     1501 cgaagccctg ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa
     1561 tggcgatttt ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc
     1621 agaagttggc ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt
     1681 ttacgaagga tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc
     1741 agttaaaaat gccggataca gcgttgatgg cgggatggag gaagagtgca tcgtggtcgc
     1801 cgattacgcg gtaaaagtgc cagatggtct ggactcggcg gcgcccagca gcattacctg
     1861 tgcgggggtc accacctaca aagccgttaa actgtcaaaa attcgtcccg gtcagtggat
     1921 tgctatctac ggtcttggcg gtctgggtaa cctcgccctg caatacgcaa agaatgtctt
     1981 taacgccaaa gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat
     2041 ggatgcagat ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa
     2101 aaccggtggc gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc
     2161 agttgatgct gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat
     2221 gagcctggat atcccacgtc ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg
     2281 cacgcgccag gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa
     2341 agtcgccctg cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa
     2401 aatccgtggc cgtatggtga ttgatttccg tcactaagag gtctttgctg cgactcccat
     2461 gttcgggtcg cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg
     2521 taggaaggaa caataatgat agtgttccca gagaagtaac gctactgaga gtaaagacac
     2581 taatgaaaat ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac
     2641 ttaatgtcag cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc
     2701 gattgctgac cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga
     2761 tcggcagttc gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag
     2821 aaaaaacggt ggttgtgtcg cgattacgcc gtctggttac gcaataaaaa atggcaccct
     2881 ataaagcgcc atttgaaact gatgacaaac gcaacattgc ctgatgcgct acgcttatca
     2941 ggcctacacg ttcacttcaa tctattgtat ttgaagggtt ttcatgccgg ataaggcatt
     3001 cacgacgcat ccgggatgaa caaagcccac gttgtctaca atatgaaaat ggcgcctttt
     3061 acagcgccat tttcagctta aagttcaaaa gagattatat cccttcttca ctctcttttt
     3121 tcgcttctgc cttctcgatt tcacgatacc agcgcggatg gtgtttcttc gcccagcgac
     3181 ggctcacctt cccttcgatc atccctttaa tcgacccttt cacccagaat gccatataca
     3241 tatggatcag gatggcgtgg atcaggatga tacccgcagc cgcgtggatc agcaggctgt
     3301 agcgaacaac ctgcatcggg aagtactgcg caaagtacgg acgccagata atcaccccgg
     3361 tcaccagcag cacgaaaatc atgctcatga tcgaccagaa catcatcttt tgcccggcgt
     3421 tgtacttacc aacatccgcc actttgtgct cattgccttt taatacttcg acaatgttca
     3481 acagccacgg aatatctttc ttatccggga tgttgtgatg cacaaaacgc acaaacataa
     3541 acatcagtgc gacgaaaatc gcaatgccga agaaggggtg caaaatgcgt cccatctgcg
     3601 gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa atcccggaca
     3661 gcgccaccag gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca ataaatttgg
     3721 tgcgcacaat cattttcgac ttactcatga tgatcctcct cgtcatcgtc cacttcctta
     3781 ttcgggccaa taccgatgta gtggaaaatc aacccggcaa aagtggcaat aaagccagcc
     3841 gctgccagcg gtttcaacgc gcctttccac aggcttaccg aggtgtcgat cttcggatct
     3901 ttcggcagac cgtgatacag ctccggctga tcggcgtgat gcagcacgta cataacgtgc
     3961 gtaccaccga ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg cgctttcagt
     4021 ttcgccacgc gctgttccgc cagctccagc atctccttct tggtgccgaa gtggatagcc
     4081 ccggtcggac aggttttcac acaagccggt tcctggccga cgctgacgcg atcgacgcag
     4141 agcgtgcatt tatatacccg gttatcctct ttgttgaggc gcggaatatt aaacggacac
     4201 ccggcaatgc agtaaccaca gccgatgcag ttttccgact ggaaatcgac aatcccgtta
     4261 gcgtactgaa tgattgcacc agcagacggg cacgccttca ggcagcccgg atcttcacag
     4321 tgcatacagc cgtctttacg gatcagccac tccagcttgc cattctgttc ggtttcgcta
     4381 aagcgcatca ccgtccagga cttggcgctc agatcggcgg ggttatcgta aaccccgacg
     4441 cagtgcccca cttcatcacg gatgtcgttc cactccgaac acgccacctg acaggcttta
     4501 cagccgatac aggtggaaac gtcgataagt ttcgcgactt ctgctttgta atcacgcacc
     4561 tgagaaggcg gcgtgatgga gttagttgcg gaccttttga taatatcctg cgtttccata
     4621 gccatctgtt cgccccctta cgccttctcg atgttgacta agaacgcttt atattccggc
     4681 gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt tagcgatata acctttacgc
     4741 gcgacaccct caaagcccca gtggattgga atacccaccg tttcaacctg ctgaccgttt
     4801 acattcagcg gtttcagacg gcgcgttacc actgccaccg cgcggataaa gcctcgcttg
     4861 ctggagacag tgacacgatc gccattatta atgcctttcg ccgccgcctg cgtttcgctg
     4921 atttccacaa actgttccgg ctgagcaatt gcgttgagca acgcgtgctt ggtccaggtg
     4981 tggaagtgct cggtcagacg ataggtcgta cccacatacg ggaactgctc ttttttaccc
     5041 atccgcagcg cgtcttgttc atacagacga acaaccgggt tagacaccac gttcggatgc
     5101 agcgggttag tgcccagcgg cgtttcaatc ggctcgtagt gttccgggaa cggaccttcc
     5161 gccattttgt tgatggcaaa caggcgtccc atcccttccg gctgcataat aaacggcccg
     5221 gttggcgtac ccggtgcggc attgccgaag tccggaatat cgttacccgt ccacttgctg
     5281 ccgttccact ggatcagcat ccgtttcgga tcccacggtt taccgttgat atccgccgaa
     5341 gcacggttgt agagtacgcg acggttgagc ggccacgccc aggcccatcc cagcgtattc
     5401 cccagaccgg acgggtcgga gttatcgcgg tttgccatct ggttaccctg ctctgtccag
     5461 ctaccggtgt agatccagca agaagatgcg gttgtaccgt catcacgcag atgcgcaaag
     5521 ctactcagca actgaccttt cttcgcaatc agcacgccat tagcatcata gagatcttcc
     5581 aacgcatagc cgttgttctc tttagctact tcgtcagatt gcggttcgtg cggctgcttg
     5641 tagttccagc tcatcttcat cagcggttct acgcctttac caccttcggc ctggtacaac
     5701 tcgcgcagat gatggtagat acccgccaga atttcgccgt cgttacgcgc ttcgcccggc
     5761 gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg agttagcgat agaaccatcc
     5821 tcttcagcaa agcaggtcga aggcagacgg aatacttcag tctgaataga cgccggatcg
     5881 acatcgttcg actcgccgtg gttctgccag aaggtagagg tttcagtcac cagcggatcg
     5941 ataaccacca tgtacttcag tttgctcagg cagctcacca ctttgttttt atccgggaag
     6001 gacgcaaccg ggttaaagcc ctggcagaaa taaccggtaa ctttgccttc atccatcatg
     6061 ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc atagccccag
     6121 ttgttctctt tctgcgcggc atcgccatag aaagatttca tcaggctaac gaagaacttc
     6181 ggatagttgc tccagtagtt cacctgatca gccagcgtcg ctttcggcgt gttcgcttcc
     6241 agatacgact gcaaatcaac ctgtttttct gacggcagcg tcagataacc tggcaggctg
     6301 gtagagagca gacctaagtc agtcagcccc tgaatgttgg agtgaccacg caatgcgttc
     6361 acgccgccac cggccatacc catgttaccg agcagcaact ggatcatcgc catagtacgg
     6421 atgttctgcg cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa ggtggttgtg
     6481 cgatccggtg cgctggtgga ggccagcact tcacacactt tcaggaagtc ggcttttggc
     6541 gtaccacaga tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc tttcagcagg
     6601 ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc gttttcatcg
     6661 aactgatagt tccaggacga tttgtcgtac tggcgttttt cagcgtcata accgctgaac
     6721 aggccatctt cgaaagcaaa atcatcacgc accagtaggc tggcgttggt gtaatgttta
     6781 acgtattcgg cgttgatttt gttgttttcg atcaggtagc gcaaaatgcc agacaggaac
     6841 gtaatgtccg taccggaacg aataggtgcg taaatatccg ccacagaagc ggtacgcgta
     6901 aaacggggat cgacaacgat caaggttgca tcgttgttgt ttttcgcttc catcgcccag
     6961 cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca ttaccattac gacgttagcg
     7021 tttttgatat ccacccagtg gttggtcata gcaccgcgac caaatgttgg agcaagactt
     7081 gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta ccgccagcat cccgagggag
     7141 cgggcaaatt tctgggtcag catcccggtt tcgttgctgg caccggaggc acacagcata
     7201 ccggtagaaa gccaacggtt taccgttacg ccctgctcgt tcttttcaat aaagttagcg
     7261 tcacggtcag ctttcatcag cttcgcaata cgggagaatg cttcttccca gctaatgcgc
     7321 tgccatttgt cagaacctgg cgcacgatat ttcgggtagc gcagacggtt ttcactgttg
     7381 acgtaatcca gcaaaccggc ccctttcggg cacagcgcac cacggcttac cggatgatcc
     7441 gggtcacctt caatgtgata aatcgcttct ctggcgtttt ttgcgccatc acccaggcta
     7501 tacatcaata gcccgcaacc tacggaacag tatgtgcagg tgttacggat ctctttagcg
     7561 cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa gcccaatgcc
     7621 gctaccgttg ttccagccat accgcccgcg cagattttaa aaaattgtct gcgactgacg
     7681 tccattgctt tcctcttttt tcaggggtat tactgcgcga ggaaactaac agtaaaattc
     7741 tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa tccgcctgcg
     7801 caaattgacc tacctcaata gcggtagaaa aacgcaccac tgcctgacag gccagttaaa
     7861 aaaatgctat aaaattcagc ttaattttta acggcaagag agacaaaaca gcgagcatga
     7921 cacgacaaaa agcaacgctc atagggctaa tagcgatcgt cctgtggagc acgatggtag
     7981 gattgattcg cggtgtcagc gaggggctcg gcccggtcgg cggcgcagct gctatctatt
     8041 cattaagcgg gctgctgtta atcttcacgg ttggatttcc gcgtattcgg cagattccga
     8101 aaggctattt actcgccggg agtctgttat tcgtcagcta tgaaatctat ctggcgcttt
     8161 ccttagggta tgcggcgacc cgtcatcagg cgattgaagt gggtatggtg aactatctgt
     8221 ggcccagcct gacaattctc tttgccattc tgtttaatgg tcagaaaacc aactggctga
     8281 ttgtaccagg attattatta gccctggtcg gcgtctgctg ggtgttaggc ggtgagaatg
     8341 ggttacatta tgatgaaatc atcaataata tcaccaccag cccattgagt tatttcctgg
     8401 cgtttattgg tgcgtttatc tgggcagcct attgcacagt aacgaataaa tacgcacgcg
     8461 gatttaacgg aattaccgtt tttgtcctgc taacgggagc atgtctgtgg atttactatt
     8521 ttctcacgcc acaaccagaa atggtattta gcacgcccgt catgattaaa ctcatctctg
     8581 cggcatttac cttaggattt gcttatactg catggaatgt cggtatattg catggcaatg
     8641 tcaccataat ggcggtaggc tcgtatttta cgcctgtact ttcctcagcg cttgcagccg
     8701 tgctgctcag cgccccgctg tcgttctcgt tctggcaagg cgcactaatg gtctgcggcg
     8761 gttccctgct ctgctggctg gcgacacgtc gtggttaaaa taattgccgg gtctacggcc
     8821 cggcagttaa tcagataaat tccgacaata ttaaaaaata aatttcaaat accagtaatt
     8881 caccttatat ctcatttcga cttcgatcac atatcgataa ataatattat tctatttttc
     8941 tcattgtttt taatcaccat tctattttaa aaacagtatg tcatttataa atgacgccgt
     9001 ttaaaattcg tttagaaaat acgtttaacg ataattaaaa ggattctgtt aaatgaaatt
     9061 aaaaatagtt gcggtggttg taactggttt gttagctgcg aacgtagcac acgctgccga
     9121 agtctataac aaggatggta ataaactcga cctttatggc aaggttaccg ctctacgtta
     9181 ttttactgat gataagcgtg acgatggtga taaaacttat gcccgtctcg gctttaaagg
     9241 agaaacgcaa atcaatgatc aaatgattgg ttttggtcac tgggaatatg attttaaagg
     9301 ctataacgat gaagccaacg gctcgcgcgg caacaagacc cgtctggcct atgctggttt
     9361 aaaaattagt gaatttggct ctctggacta tggtcgtaac tacggtgtcg gctacgacat
     9421 tggttcatgg accgatatgt tgccagaatt tggtggcgat acctggagtc agaaagatgt
     9481 cttcatgaca taccgtacca ccggtctagc aacctatcgc aactacgatt tctttggctt
     9541 aattgaaggg ctgaactttg ccgcgcaata tcaaggcaaa aataaacgta ctgacaacag
     9601 tcatctttat ggtgctgacg acacgcgtgc caacggtgac ggtttcggta tctcctcaac
     9661 ttatgtttat gatggctttg gtatcggtgc ggtatatacc aaatccgatc ggacaaatgc
     9721 gcaggaaaga gccgcagcta atcctctcaa tgcctccggt aagaatgcag aactatgggc
     9781 cacaggtata aagtatgatg ccaacaacat ctactttgca gctaattacg atgaaacatt
     9841 aaacatgacc acctatgggg atggttatat ctctaacaaa gcacaaagtt ttgaagtggt
     9901 ggcgcaatat caattcgact tcggcttgcg cccatcactc gcttacctga aatcgaaagg
     9961 cagagatctg ggccgctacg ccgaccagga catgattgag tatatcgacg ttggtgcgac
    10021 gtatttcttc aacaaaaata tgtcgaccta tgttgattat aaaatcaacc tgattgatga
    10081 aagcgacttt acccgtgccg tagatattcg caccgataac atcgtcgcta cgggcattac
    10141 ctatcagttc taacgtcctc tcctgaccag cccttctctg ggctggtttt tgatttaagt
    10201 gctgctgtat gactccttaa gtaaaaatga gcaattcttg atatttctgg tcattcacct
    10261 caattaatat tattccatat cctccgggga catcttacta taatatttat tgatgagtga
    10321 ttatgaccaa tattaataca gcttgtgtaa aaaataatgc cagttatcaa cttaataacg
    10381 cattacctaa caaagaaacc atctccagca atttttgtga acgactagaa caatggggta
    10441 ataaatcggt taataatggt gaagaaagag caattgccgt ggagcgaatt aaagaagctt
    10501 acaattcgaa catggcatct cttgatttgt catatcttga tttgagtgaa ttaccgccta
    10561 tcccctcgac agttaatacg ttaaatctgg aaaacaattg tctaacttgt cttgacttag
    10621 ctgacaatgc cagtctcgtt aatatcaacc tcagcgttaa taaaattaac acgataacct
    10681 ttccaaaaga accaaaactg gaaaacattt atattaatca taacaatctg gaaagtttag
    10741 atttaaaaaa tcagcattca ttggttaatc tggaagcgca aaataatagc ctaaaaaaat
    10801 gatatttctg atagttataa actgaaattt cttaatcttg attataacaa gctagcatcg
    10861 ctggatgtct cccggcaaga atctctgatt gagttaagtg cccatcacaa tatgatcact
    10921 gaccttatat tacacaatca tcccaggatg aaaacaatca ctttaaacga caaccatatt
    10981 gcacatttaa acgccaaaaa cactacaaaa ctggaatatt taaacttaag caataacaat
    11041 ttactgccaa ccaatgacat tgatcaacta atatcatcaa aacatctttg gcatgtatta
    11101 gttaacggca tcaacaatga tccacttgca caaatgcagt actggactgc agtaagaaat
    11161 ataattgatg acactaatga agtgaccatt gatttatcag gacttaattt aaccactcaa
    11221 ccaccagggc tgcaaaactt cacctctatc aatcttgata ataaccaact cacacatttt
    11281 gatgcaacca actacgatag actcgtaaag ctaagtctga atagtaatgc tcttgagtca
    11341 ataaattttc ctcaaggcag aaatgtaagt attacacata tatctatgaa taataatgct
    11401 ctcagaaata ttgatataga taggctttca tcagttactt attttagtgc ggcacataat
    11461 caactagagt ttgtgcaatt agaatcttgc gaatggctgc aatacctgaa tctcagccat
    11521 aatcaattaa ctgatattgt tgcaggtaat aaaaatgaac tcttactgct ggatctatcc
    11581 cataataaac taacaagttt acacaatgtc ttatttccca acttgaatac gttacttatt
    11641 aacaacaatt tgctttctga aattaaaata ttctatagca acttccgcaa tgttcagaca
    11701 ttaaacgctg ctaacaacca gttgaaatat ataaatcttg atttcctgac ttatcttcca
    11761 tctatcaaaa gtttaagact ggacaataat aaaataaccc acattgatac taataataca
    11821 tccgatattg gaactttatt ccccat
//
LOCUS       DAJTYM010000108        11637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTYM010000108 DAJTYM010000000
VERSION     DAJTYM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..626)
                     /gene="cheZ"
                     /locus_tag="OO575_003244"
     CDS             complement(<1..626)
                     /gene="cheZ"
                     /locus_tag="OO575_003244"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6666097.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDL"
     gene            complement(637..1026)
                     /gene="cheY"
                     /locus_tag="OO575_003245"
     CDS             complement(637..1026)
                     /gene="cheY"
                     /locus_tag="OO575_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6666098.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1041..2090)
                     /gene="cheB"
                     /locus_tag="OO575_003246"
     CDS             complement(1041..2090)
                     /gene="cheB"
                     /locus_tag="OO575_003246"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6666099.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2093..2953)
                     /gene="cheR"
                     /locus_tag="OO575_003247"
     CDS             complement(2093..2953)
                     /gene="cheR"
                     /locus_tag="OO575_003247"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6666100.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(2972..4573)
                     /gene="tap"
                     /locus_tag="OO575_003248"
     CDS             complement(2972..4573)
                     /gene="tap"
                     /locus_tag="OO575_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6666101.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4619..6280)
                     /gene="tar"
                     /locus_tag="OO575_003249"
     CDS             complement(4619..6280)
                     /gene="tar"
                     /locus_tag="OO575_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6666102.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6425..6928)
                     /gene="cheW"
                     /locus_tag="OO575_003250"
     CDS             complement(6425..6928)
                     /gene="cheW"
                     /locus_tag="OO575_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6666103.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(6949..8913)
                     /gene="cheA"
                     /locus_tag="OO575_003251"
     CDS             complement(6949..8913)
                     /gene="cheA"
                     /locus_tag="OO575_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6666104.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFALGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(8918..9844)
                     /gene="motB"
                     /locus_tag="OO575_003252"
     CDS             complement(8918..9844)
                     /gene="motB"
                     /locus_tag="OO575_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6666105.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(9841..10728)
                     /gene="motA"
                     /locus_tag="OO575_003253"
     CDS             complement(9841..10728)
                     /gene="motA"
                     /locus_tag="OO575_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6666106.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(10855..11433)
                     /gene="flhC"
                     /locus_tag="OO575_003254"
     CDS             complement(10855..11433)
                     /gene="flhC"
                     /locus_tag="OO575_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6666107.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11436..>11637)
                     /locus_tag="OO575_003255"
     CDS             complement(11436..>11637)
                     /locus_tag="OO575_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6666108.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2636 a         3269 c         2851 g         2881 t
ORIGIN      
        1 aacaaatcgt ccacctgatc ctgactggct accacgccgg ctttgctggt atcgacctga
       61 ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc cgggatgttt
      121 tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat catccgctta
      181 ataacctgcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg
      241 ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc tgttaccagt
      301 tcacgggcgt cggcaaggtc aatcggatcg gcaaaccagt catcccaacg ttgggttaac
      361 gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac actgttcagc
      421 gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg
      481 gggatggctt ccgccgcttc ggcaatggcc tgaaccagcc ccagttcccg caaactgtcg
      541 cgcagcatat gcgtcaggct gccgatgcgc gcaatgatat cgccagctga atgctcgtca
      601 gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt tctcaaagat
      661 tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat agccactggc
      721 ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca ccattaacac
      781 tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca ggccatccat
      841 attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca acttattgag
      901 agcgtcgaca ccatcttccg cttcctcaac attattgaat cccagctctt tcagcaggtt
      961 acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa gttctttatc
     1021 cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat tgttgccaac
     1081 atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat attgatggcc
     1141 tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt ccatgccccc
     1201 gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt caggatcacc
     1261 ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc taccgaaggc
     1321 cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc gccactacgc
     1381 gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa gacacgttct
     1441 ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc ggcaaaagag
     1501 cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga aagcggcaac
     1561 ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt
     1621 ttttcagaac tcaacaacgg ccctgccttc agcgttgtcg gtgctgacaa tggcttatgt
     1681 gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc gctatacgcc
     1741 agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc ccccagctcc
     1801 agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat cacaacgggc
     1861 attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg cggcatttca
     1921 acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc aaccagcgga
     1981 tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc
     2041 tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat cgttaatcct
     2101 tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcgagg tggctaaagt
     2161 tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca aagcggcgca
     2221 aaatctcctg ctgggtagtt tgatcgaagt agatcatgac gttacgacag aagatcgcat
     2281 caaacggccc cggcacggtg tactgtttcg ccagtaaatt cagcggggca aaatcaacat
     2341 agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc cctcgcatga
     2401 aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga tagataccgc
     2461 ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc cagcgtccgg
     2521 gcacggtgcc caatgtgtca gccagagtca tcgcaatgct gtacggctct tcgccggtcg
     2581 aagccgccgc gctccatacg cgatcctcgc cagaacggcg acgtgcgtga tccgcgagca
     2641 gagggaaatg atgagcctca cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa
     2701 acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa tgaccgaaat
     2761 ccgtcagtcc cagagaacgc aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat
     2821 ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc cgaaaatgcg
     2881 cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc ccacagggca
     2941 gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca atttgtaact
     3001 gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc acacgcgacg
     3061 aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct tctaccagcg
     3121 aggagttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc
     3181 cccgccgttg ttcttccgac gccgaagcaa tttctcccat aatgtcgttc acgcgggtca
     3241 ccgaactgac aatatcggtc atggtcgcgg cggcgttatt caccagtttc gaaccctgct
     3301 ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc tgcgcgctac
     3361 ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc tgctctccgg
     3421 cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa gcaattccgt
     3481 cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc tgcatggtgt
     3541 gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca
     3601 gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt aattgctcca
     3661 tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt gagagatcgt
     3721 tattgcctgc gacaatctcc gcgatcccaa tgtgcatctc atggcttccc ttacgcacat
     3781 cactcaccgt cccacgcaaa gcctgctgca tggtcttcag gctggcaaaa atggcggtga
     3841 tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca atgctgtcaa
     3901 aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac cacagcgcac
     3961 tgctgatgta gatcgccgca acaataatca tgctgataaa caccagcgcc gaaatctgat
     4021 agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc agctgccagg
     4081 cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg aggaaatccg
     4141 aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa
     4201 aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca
     4261 taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc gtcatcaacg
     4321 ttttaatgtc atccgccgga tagctaagcg ctgtcagagt gcctgctttg ttcagcgcgg
     4381 tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc tgattgctct
     4441 gctcgccgtg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac
     4501 tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa
     4561 tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta aaaactcgtc
     4621 aaaatgtttc ccagtttgga tcttgttcag taattcgcag tcgtggctgt gccggtggtt
     4681 gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg gctgccagac
     4741 ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca gctgcggcgg
     4801 cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt tccgaaaccg
     4861 ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca atctcgccca
     4921 taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc ccggcgcttt
     4981 cgaccagcac cgaaccggta tcaatgcgcg agacggagtc ttcaatgagg gctttgatct
     5041 cttttgccgc cagggcgctg cgactggcaa gattacgcac ttcacccgcc accacggcaa
     5101 aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc gcgaggatat
     5161 tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc ttcgaactat
     5221 cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg tgctgggcgg
     5281 tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg ttttgcttca
     5341 ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat gcctgctgtt
     5401 cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg
     5461 catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa
     5521 cgctctgcgc caggtcaccc atttcactgc gcccgtcaat ggtcagggta ttcgccaggt
     5581 taccaccggc gatttcgcaa atgtgagcaa taatttttgc cagcggagta agcaacatac
     5641 ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg ataaccgcca
     5701 gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg cgatacagtt
     5761 tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc attccctggg
     5821 ttggctgagc gaaataagct ccagtattgc cataatcaag ataatcaatc agttcagtta
     5881 acgctgtgta atagttttta tatttttcat caaaattacg gctggtagcg accatttcag
     5941 gtaacggtgc catgcttttg aattttttat aatgcgtcgc ggcctgggcc aatgttttcc
     6001 tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa tccatcatca
     6061 tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc caggttgacg
     6121 tcagctcgcc ctgctgttcc cgtaattgat tggaaaccac aaagctcttc tggctatggt
     6181 gaagggaaga aaaaaacaaa ctgccggaaa taagctgtaa cagtgcgaat acccccagca
     6241 ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt cctgaaaaca
     6301 agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca cccgccgcga
     6361 aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga ggaaatcggg
     6421 agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg ctgttcagca
     6481 gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt ccagtgagat
     6541 attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc gccgtcaatg
     6601 aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg agattcagga
     6661 cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta attcgtaagt
     6721 caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac gctggcgtgt
     6781 tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg atatcaatac
     6841 cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc tcgctggcca
     6901 gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc aggcggcggt
     6961 gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa caatcagtgc
     7021 cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc gatagttact
     7081 ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca gcaaggcgta
     7141 gcggcgaccg ccactttgta agatcaccac aattccctgg gtggcttcgg ttttcgcgcc
     7201 cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc gcacttccag
     7261 cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca gtgattccat
     7321 aacagcattc agcggcagaa tgaaaacttc atccgcaacg cgtacggaca tgccgtcgag
     7381 gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtaccct gcttcgactg
     7441 gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca tgccgacgcc
     7501 gcgcccggag acgtcggtga cctgctctgc cgtggagaag cctagtgcaa atatcagcat
     7561 tgcaacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg ccgcttttgc
     7621 cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc aaatgttgcc
     7681 gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg cggcgagccg
     7741 tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg tcagcgggtc
     7801 gataatgcgt tctatcaggc tcttgtcgag ctcggtggaa ctgcccacca gcgtcagttc
     7861 tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc gactaaagac
     7921 atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac gggcgttacg
     7981 ttgtaactgc cccatgctgg taatcaaatc accatgatta accgggtcca gttcgctgga
     8041 acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa ttaattgatc
     8101 aaccttttct accgctacac ggatgctggt ggattcactg ctgcgcgtcg ttttttcccg
     8161 ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcacaggtg gggtagctat
     8221 ttttggcgaa acttctactg tttcaaaggt aatctgatcg gcttcaatca caaaacagag
     8281 taccgctgtg atgtcatctt cggcgatatc gcccggtaat attgccgaga gagaatccgc
     8341 ccctttcacc acgtcattta acgttgtcag atgccccagc tcttcttcca gcaggtcgac
     8401 ttcgccggcc ttcaggcgcg aaaggataat tcgtcgcggc aactgactgc gactttgctc
     8461 atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg gcgtttcgcc
     8521 tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgtagc tggctgcatc
     8581 cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct tcgtttccaa
     8641 aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt catcgagcag
     8701 gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc ctgcccctcc
     8761 tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg gcgcttccgg
     8821 ctgcaaaacc agcagatgct gctccatatc agccaacagt tcgtccgctt catcaaaaaa
     8881 tgtctgataa aaatcgctta tatcgatgct cacgctgtca cctcggttcg gctgatggca
     8941 ttgtgggaac actgacctgt ggtgcaacct caggtttttc tagggcgctt actggctcat
     9001 tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc agtaccaaca
     9061 ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc atcgttgccg
     9121 ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc agttcgcggc
     9181 gggatgcatt ggcccgatcg gcagaaagct cccagttgct gtaacctttc tcaccgctgg
     9241 cgtagggaaa atcatcggta tgacctgaaa ggctaatacg gttgggaata ccgttcagca
     9301 caggcgcgat ggcgcgcaga atgtcgcgca tatagggttc gacatcggca ctgccggttc
     9361 taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc tggaccagat
     9421 cgattttgag atggagacgt aacgcccgca gtttcggatc ggactctatc aactggtcga
     9481 gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcagc tcttcgatgt
     9541 tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg ccgggaattg
     9601 ggctttcact attggaaatg cgatcgccgc ccgtaaccgc tgtcgccagt ggagtccgga
     9661 agtactccgc aatctgaatc aactcttttg ggctggagat ggagatcagc cacatcacca
     9721 gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac gatccatgtg
     9781 ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc gcttgattct
     9841 tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca catgctcttc
     9901 cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa actcaacggc
     9961 gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc actgcatcat
    10021 tttgctggtt tcggcgcttt tctgacgtaa aacactcgct agtggggaaa taaatccgta
    10081 agccaataaa ataccgagga aagtccccac catcgcatgt gcgataagcg cccccagctc
    10141 ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag ccgcgacaat
    10201 accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga cttctgcctc
    10261 gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg tgttcatgtg
    10321 accgctgata atcaggcgca gataatcgac gataaaatca agcatgacgc tatccgcgag
    10381 gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat cacgttccag
    10441 cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag ccagcagatc
    10501 catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg ctttcagcgt
    10561 gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg caccggcaat
    10621 aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca tcaaataacc
    10681 gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga catcatcctt
    10741 ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat tggggaaaaa
    10801 aaaagcagcg gtacgtcgtt accgctgctg gaatgttgcg ccacaccgta tcagttaaac
    10861 agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat tctgggaaag
    10921 tttacgtctt tttactgccc gggatggcgg ttgacataag ctgcaggcaa agctgccaac
    10981 aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg aaagttgcag
    11041 taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc
    11101 ttcttctgct tgtgggcact gttcaaggta taaacggtag gctttgatca ccgcatcgac
    11161 gccattacac aaaccggttt tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg
    11221 aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc ctttcggcgg
    11281 tgggcttccg cgcagttctt tataaagttt tatcaggcgt ccgcgactta actgtgtttc
    11341 gctttccagc atctgcaaac gagcgcccag ggtgatcaat tccattgcca gctgaatatc
    11401 ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt gcgcagcgct
    11461 tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt
    11521 tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg gtggctgtca
    11581 aaacggaagt gacaaaccag ctgattggtt tctgccagct taaccatttg cggaaga
//
LOCUS       DAJTYM010000109        11555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTYM010000109 DAJTYM010000000
VERSION     DAJTYM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..482)
                     /locus_tag="OO575_003256"
     CDS             complement(<1..482)
                     /locus_tag="OO575_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6666109.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     "
     gene            complement(513..1205)
                     /gene="fimC"
                     /locus_tag="OO575_003257"
     CDS             complement(513..1205)
                     /gene="fimC"
                     /locus_tag="OO575_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6666110.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1425..1967)
                     /gene="fimA"
                     /locus_tag="OO575_003258"
     CDS             complement(1425..1967)
                     /gene="fimA"
                     /locus_tag="OO575_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6666111.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2448..3314
                     /gene="folD"
                     /locus_tag="OO575_003259"
     CDS             2448..3314
                     /gene="folD"
                     /locus_tag="OO575_003259"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6666112.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3316..3528
                     /gene="ybcJ"
                     /locus_tag="OO575_003260"
     CDS             3316..3528
                     /gene="ybcJ"
                     /locus_tag="OO575_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6666113.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3636..4145
                     /locus_tag="OO575_003261"
     CDS             3636..4145
                     /locus_tag="OO575_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6666114.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4181..5566)
                     /gene="cysS"
                     /locus_tag="OO575_003262"
     CDS             complement(4181..5566)
                     /gene="cysS"
                     /locus_tag="OO575_003262"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6666115.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5740..6234
                     /gene="ppiB"
                     /locus_tag="OO575_003263"
     CDS             5740..6234
                     /gene="ppiB"
                     /locus_tag="OO575_003263"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6666116.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6237..6959
                     /gene="lpxH"
                     /locus_tag="OO575_003264"
     CDS             6237..6959
                     /gene="lpxH"
                     /locus_tag="OO575_003264"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6666117.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7077..7586
                     /gene="purE"
                     /locus_tag="OO575_003265"
     CDS             7077..7586
                     /gene="purE"
                     /locus_tag="OO575_003265"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6666118.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7583..8650
                     /gene="purK"
                     /locus_tag="OO575_003266"
     CDS             7583..8650
                     /gene="purK"
                     /locus_tag="OO575_003266"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6666119.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8787..9680)
                     /locus_tag="OO575_003267"
     CDS             complement(8787..9680)
                     /locus_tag="OO575_003267"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6666120.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9677..10324)
                     /locus_tag="OO575_003268"
                     /pseudo
     CDS             complement(9677..10324)
                     /locus_tag="OO575_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10327..>11555
                     /gene="allC"
                     /locus_tag="OO575_003269"
     CDS             10327..>11555
                     /gene="allC"
                     /locus_tag="OO575_003269"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6666121.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQ"
BASE COUNT         2791 a         3006 c         2963 g         2795 t
ORIGIN      
        1 aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc aaagttaatt
       61 gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg ctgggctaac
      121 tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt aaaacagggc
      181 attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac gatatcctgt
      241 gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc aggttgatga
      301 ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa taaaaatgcc
      361 gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag agatgaataa
      421 caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt gggtattttc
      481 ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc tctgaccggg
      541 gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac tcctgagggt
      601 aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt ttggctgccc
      661 acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt gatatagtta
      721 ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg taattgaatt
      781 ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt cctgccgttc
      841 aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag agactcacga
      901 tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct tttgggttca
      961 ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct gctgttttca
     1021 atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca taccgcagtc
     1081 tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc tccggcagca
     1141 tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt tattttagtc
     1201 atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat aaagaaatga
     1261 ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg catgatacaa
     1321 agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg gtacattact
     1381 ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc gtatctcata
     1441 ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt gcctttataa
     1501 cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt tgagaagcta
     1561 ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc gacacccgtt
     1621 gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat tgcatcagcc
     1681 tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc gttcaactga
     1741 atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc ggtacgatat
     1801 tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc agcattcacc
     1861 agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact acccgtagcg
     1921 gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata tattccttca
     1981 atctcattta tcgactccac atccgtatat aaccgattac tttatttaag acactgatag
     2041 tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca ctctattcta
     2101 aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac cacaaatata
     2161 gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg tatttcttaa
     2221 attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg ccatcatgta
     2281 cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac cttcgcaaga
     2341 ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct tctgacaaaa
     2401 tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg gcagcaaaga
     2461 ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa aaagttcagg
     2521 ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg ggtagtaacc
     2581 ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc gggttcgtct
     2641 cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag cttatcgatg
     2701 cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta ccggcgggta
     2761 ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac ggtttccatc
     2821 cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc accccgcgcg
     2881 gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac gccgtggtga
     2941 ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc gcaggttgca
     3001 ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa aatgccgatc
     3061 tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc aaagaaggcg
     3121 caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg ggcgacgtcg
     3181 tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc ggcgttggcc
     3241 cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa tatcatgatc
     3301 cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt tgagctgtgc
     3361 gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat cgcgattgcc
     3421 gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa aatcgtcgcc
     3481 ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc cctttcccgg
     3541 cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc gttcatccat
     3601 gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt acgcacgccg
     3661 ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt gccggaatca
     3721 tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt gcttacggca
     3781 atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt gtcccgctct
     3841 tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc tggctgtttt
     3901 taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc ggtaagggcg
     3961 ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag
     4021 tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg attatttccg
     4081 aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac
     4141 gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga cgccaggtgg
     4201 tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca cgtgccgcat
     4261 ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa
     4321 tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt
     4381 gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg
     4441 ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac agcacggaat
     4501 aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt
     4561 cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag aggcgctcca
     4621 gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc
     4681 ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca tcgcgcacgg
     4741 taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt
     4801 gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg atttcgttct
     4861 cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg ttacccagct
     4921 gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg ccccacggag
     4981 acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc atccggttgc
     5041 gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga tcctgacgcg
     5101 acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg ttgtccgcca
     5161 cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt
     5221 gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca
     5281 tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca ttggcgcgtt
     5341 tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga
     5401 aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac
     5461 agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg tgaataggct
     5521 taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt
     5581 agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata
     5641 cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa
     5701 ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat
     5761 cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc
     5821 ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc
     5881 tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg
     5941 atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc aatggcacgt
     6001 actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc
     6061 ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt
     6121 gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa cggtatgcac
     6181 caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg
     6241 cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt
     6301 ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt cttggcgatc
     6361 tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag atggcggcag
     6421 cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc aaccgtgatt
     6481 ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg gaagaaaagg
     6541 tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg
     6601 acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc
     6661 tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg aacagcaaag
     6721 aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct gtggtcagtg
     6781 cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc ccggcggtgc
     6841 atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc tggcatacgg
     6901 aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt ccgttttaaa
     6961 aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg
     7021 ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt
     7081 cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta
     7141 ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg
     7201 tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg
     7261 gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc atgattgccg
     7321 ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg
     7381 atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg
     7441 gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata
     7501 aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa gtgctggaaa
     7561 acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg
     7621 ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg tcgggctgga
     7681 cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg
     7741 gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga accgcgatgt
     7801 gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc tccacctgcc
     7861 gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt
     7921 aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggag
     7981 tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat gtattgtcga
     8041 gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct ttgatggcag
     8101 caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt
     8161 cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat
     8221 tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg
     8281 tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaacgg
     8341 tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca
     8401 accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga
     8461 ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg
     8521 taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga
     8581 agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa
     8641 gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt
     8701 accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt
     8761 gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc
     8821 agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg gcttctgaca
     8881 taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct
     8941 aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat
     9001 accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac
     9061 gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc cgtcacaggc
     9121 acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct
     9181 tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca
     9241 tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa
     9301 accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg
     9361 cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc
     9421 atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata cggttctacc
     9481 tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca
     9541 atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg
     9601 cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga
     9661 gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca
     9721 gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg
     9781 tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt
     9841 gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg
     9901 aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac caggccgcca
     9961 gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct ttacccagcc
    10021 agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga cgaaactggc
    10081 gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa agtccggttt
    10141 cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc ggcgtaatac
    10201 gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga atcccttgag
    10261 ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg aattgcgtgc
    10321 ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct ttcctctttt
    10381 ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg gctggaaacc
    10441 cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt cgatgaagtg
    10501 gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt tctgagcggt
    10561 tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg cgcgctggcg
    10621 gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg tacggtcgaa
    10681 gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg gggcagtaaa
    10741 aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc caaaggaaat
    10801 agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc actaactccg
    10861 cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt gctggaaagt
    10921 aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta tacggtgacg
    10981 ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg tgatacggtt
    11041 tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat gggcgatccg
    11101 ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt ggtgccgggt
    11161 aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg cgatttcacc
    11221 caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg tattgatatc
    11281 gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc caccctgaca
    11341 gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc cgggcacgac
    11401 gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag catcaacggg
    11461 atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg ggtcaaaacg
    11521 ttggcactca tgctttatca acttgcctgg cagga
//
LOCUS       DAJTYM010000110        11464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTYM010000110 DAJTYM010000000
VERSION     DAJTYM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..673)
                     /gene="uxaB"
                     /locus_tag="OO575_003270"
     CDS             complement(<1..673)
                     /gene="uxaB"
                     /locus_tag="OO575_003270"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6666122.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEV"
     gene            complement(900..2318)
                     /locus_tag="OO575_003271"
     CDS             complement(900..2318)
                     /locus_tag="OO575_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6666123.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(2472..2816)
                     /locus_tag="OO575_003272"
     CDS             complement(2472..2816)
                     /locus_tag="OO575_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6666124.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(2816..3742)
                     /gene="glsB"
                     /locus_tag="OO575_003273"
     CDS             complement(2816..3742)
                     /gene="glsB"
                     /locus_tag="OO575_003273"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6666125.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(3806..4741)
                     /locus_tag="OO575_003274"
     CDS             complement(3806..4741)
                     /locus_tag="OO575_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6666126.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            4821..5936
                     /gene="yneK"
                     /locus_tag="OO575_003275"
     CDS             4821..5936
                     /gene="yneK"
                     /locus_tag="OO575_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6666127.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6086..7276
                     /gene="ydeA"
                     /locus_tag="OO575_003276"
     CDS             6086..7276
                     /gene="ydeA"
                     /locus_tag="OO575_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6666128.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(7301..7966)
                     /gene="marC"
                     /locus_tag="OO575_003277"
     CDS             complement(7301..7966)
                     /gene="marC"
                     /locus_tag="OO575_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6666129.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            8178..8612
                     /gene="marR"
                     /locus_tag="OO575_003278"
     CDS             8178..8612
                     /gene="marR"
                     /locus_tag="OO575_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6666130.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            8632..9015
                     /gene="marA"
                     /locus_tag="OO575_003279"
     CDS             8632..9015
                     /gene="marA"
                     /locus_tag="OO575_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6666131.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9047..9265
                     /gene="marB"
                     /locus_tag="OO575_003280"
     CDS             9047..9265
                     /gene="marB"
                     /locus_tag="OO575_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6666132.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(9296..10195)
                     /gene="eamA"
                     /locus_tag="OO575_003281"
     CDS             complement(9296..10195)
                     /gene="eamA"
                     /locus_tag="OO575_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6666133.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            10459..>11464
                     /gene="ydeE"
                     /locus_tag="OO575_003282"
     CDS             10459..>11464
                     /gene="ydeE"
                     /locus_tag="OO575_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6666134.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     T"
BASE COUNT         3036 a         2951 c         2601 g         2876 t
ORIGIN      
        1 ccacttcatc gcgcggataa ccggtaacga tacggtccac cagcgtagaa cagaagctgt
       61 tagcctgatc cagccattga ataaatgctt ctggcagtgc ccactcttgc gcatagcgca
      121 gcaccagttc acgcaacgcg tcgccattgt agtcaatcaa ctcacacgga atgatgatcc
      181 aacctttatc cagcgcaccg ttgaaatggc taaagcgttc gaacagcaga cgggtcagtt
      241 ttgccggata gcttaccgct ggcgcgtcat cgaatttatc gcccgcatgg tagctgatac
      301 ccgcttcggt ggtgttagag aaaacaaagc gcatttccgg gttgtgcgcc agtttcagga
      361 attcatcgta ttcactgtag acgctgattt cacgattaac cgagcgaatc agacgcgcgt
      421 cgctgaccgc ttcccccttc tcattcaggc cacggataat ggtggtgtac agaccatcct
      481 gcgtgctcag tgacggcggg aatgaagttt caatcggacg aacaacgacc acgccagaat
      541 tcagatcggt gtgctcattc aggagatcga tttgccagtc aacaaaggcg cgcaggaagt
      601 taccttcacc aaactgaatg atacgttctg gatactgtgc accgggaaaa tcgcgacgat
      661 ttagtgtttt cacaatgggt tcccttctga ttagtcatac aacccgtttg aattggtgtg
      721 acaggttagc aaactttaat acgccgaacc cctgttttga tcaactcctg atgattaatg
      781 agcagtttta tgagaaaagt gtggcgcgga tcatggttta atcgaggaaa tacgcctttt
      841 cctggatcat aaagtggtag aacacattgc attcaaatcg cgcggaatga ataaagatgt
      901 cagacaactt cctcaccgta acgcatagtg ctggtacggt tgcgcccatc tttcttcgag
      961 cgatacagac acgtatcggc ttcgaccatc aatttattaa aatcatcggt cagcgttcgg
     1021 tacgatgcgc gaccactacc gacgccaata cttaccgtga gataaagcgt tttttgttgc
     1081 caggtgaatg gttgcagttc aacgccttta cggatttttt ccgccattag cagaccatcg
     1141 acaggattca ccgacggcac tgcaacagca aattcttcgc cgcccatgcg cgccaccagc
     1201 cccttatcac cgacaatctt ctgaatatgc cgggcaaaca cgcttaacac tttatcgcca
     1261 cattcatgtc catagttatc gttaatgctt ttgaaatagt cgatatcaag cagcatgacc
     1321 gtcagatgtt gtgtctgttt cagcgatgga cttttcagcg cctcataaag accggaccgt
     1381 gagtaaacct gagtcagaaa atcaaagtcg gctcgcagcg caacttgctt cattagcaaa
     1441 ttgatcgctg ccacgctaaa agaaaccata attgggcata tcgccatcgt ggcaataccg
     1501 agacgtgcgg agaacatttc cggaatggag aacggcgaac cgaccgagat atcaatcacc
     1561 gaatttgcca ccagtacgat ttccaccgca ccggtgacaa aggtcaacag acatgttacc
     1621 tgcggcgtat agcgcactgc acaccagatt aatgcaggca gcgggaacgc cagactcccc
     1681 gctccgccaa tgactaccga ggcaataacc gacacaataa acgcgatggc gggcatcatc
     1741 tgttctgctt taaagcgcgg cagcactcca ggaatagcca acgtcagcat acaaggcacg
     1801 atcaacacgc ccgttgagaa ttgctcactg aaccagtccg caagcaaagg ccagaaatcc
     1861 agactatcaa tactgaccga accaatcgcc ccgacaatag cgcataataa tgcggcaatc
     1921 agacagtaat taaatagccg taaagcactt actggctcat acttattttt ccccagacgc
     1981 ttatcccgca cgaccagtaa ggcgacggta acaataaaca tcatattgga gaaattgata
     2041 accagtgaaa ccagtcccca ttcggtggtt atggcatcat aaaccagcat cgccacataa
     2101 ctgatcgcat agtaatgcag tcgattaagc cagacatagc gggcaaacac ccccgccatt
     2161 acgccattca gcggccagaa aagagaaagt tcgtgaacca gccgtaactc tgcaccaata
     2221 aaatagaaca gcgtggttag cgcaaaaata gcgatgctat tacgtagcag atgaccttcc
     2281 tggaacaaac ggaaagttga aatggggtgt acatgcatta attatctgtc catacaattg
     2341 cgggtggcaa ttatctcaac gtgataagta aattctcata aaggatagca tgttcgctaa
     2401 cagcggcggc aaataaaggt accctgggaa tgctatttta tcgttttccg caattctcat
     2461 ggctgattca tctatacaac cagccagtga tagattaccg ccacgatata acgttgttgt
     2521 tcttcgctta acgaaacacc ctgcggaatg ttatgccact tagccagaca accgcgacag
     2581 caggtcgcgg tggcgtgctg agcgataaac accggatgcc cgcgcatggg ggtttgctta
     2641 ccgtcattag ccggtaatgc cggagcaaga cgtttagcga caaaatccgc cgcatgttgt
     2701 tcgatgactg gcgcgccttt ctccaggcaa tactgacgct ctttcatgcc cagacgaaag
     2761 cgagagcgaa attttgaacg ggataaacgc gcgaagagtg gatcgagaga ctgcattaat
     2821 aaaccgaacg ccctaactgt ttcgtcaatt gttcaaggac ggcgataccc gcaagcgagt
     2881 tacctgcatc atccagttcc ggactccaga ctgcgatggc catttcatgc ggaacaatcg
     2941 ccacaatacc gccaccaacg ccagatttcg ccggtagtcc cacccgccag gcaaactccc
     3001 ccgcgttctg gtacatacca ctggtcgcca tcagcgcgtt aatttgccgc gcctgcattg
     3061 gcgtcaccac aggttcatca atatgaatag ctttcccctg attagccaga aagacaaacg
     3121 tccgggccag ctctacacag ctcattttca gagcgcagta atgaaagtag ttttgcagaa
     3181 cggttgtcac gtcatgatgg aaattgccaa acgacttcat cagccaggcg atagccgcat
     3241 ttcgcgcgga atgttcaaat tcggaacgcg ctaccaccgt atcgtatgaa atatcagaca
     3301 caccgcttaa gccgcgcacg acttccagca tacgttgccg tggtgcgctt agtcgccctt
     3361 gcaacatatc gcagaccacc agcgcaccgg tattaatgaa cggattacgc ggaataccct
     3421 gttccatttc cagttgcact aaagaattga acggtgatcc agacggatct ttgccgacgc
     3481 gttgccagat ttcctcttcg gagtaatgac gcatggcgac aacgagactc agcactttgg
     3541 aaatagactg aatggaaaaa cgttcttgcg cgtctccggc ctgaaaaagc tgtccgtcaa
     3601 cggtacagat agcaatcccc aatcgggaac cgtctactgt agccagcgcc ggaatataat
     3661 ccgcgacttt accctgacca atgagcggcc gcacttgccg caagatgttt tctaaaattg
     3721 cattatccat ggcgactgcc actttctact cctggaccgc aggtctgaaa agacctgcga
     3781 gtatatcaga gctgaatatg tcgcgtcaga tccggtcttt ccacactgtc tggatattac
     3841 agaattcgtg taagccgaaa tgggaaagct cacgaccaaa gccactcttt ttcacgccac
     3901 caaaggccac tcgcgcgtcg ctggcacaat aaccattgat aaacacccca ccgcattcca
     3961 gacgtgccgc catctgtctg gcctgtgttt cgtcggtggt aaaaatggtc gctgaaaggc
     4021 cgaactcact atcattagcc agttccagcg catgttctgc atctttcgca atggtgattg
     4081 cagcaacggg gccaaacatt tcttcccgaa acgcggtcat ttctggggta acattcgcca
     4141 gaaccgttgg cggatagtag ttacctgctc cagccatctt ttccccgccc agtaacaaac
     4201 gcgcaccctg cgccagggtt ttctccacct gatgatgcag ctcatcacgt aaatcaaaac
     4261 gagccattgg tccgagagcg ttctcttcat cacggggatc gcccattttc aaggctgccg
     4321 cagctgacac aaaacgttcg gtaaatgccg aagcaattcc ctcttcgata ataaagcgtt
     4381 ttgccgctgc acaaacctgt ccggtattct gataacgtcc ggctaccgcc gctttaaccg
     4441 ccagttccag atcggcatcg ttaagcacaa taaatggatc cgaaccgccc agttccagta
     4501 cgcatttttt cagtgccgct ccagcacgaa cacttccggt caccgtgaca gcagcaatgc
     4561 gcgagtcttt aatcatctga ctgacaccgt cgttgtcggc attcagccag ccatatacgc
     4621 cttgcgggat tcccgcatct ttaaacactt gggcaatgag ctgagcacag cccatcacat
     4681 tcggcgcatg tttaagtaag tagccgttac ctgcaagaat gatgggaaca gcgccacgca
     4741 tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt tccgatgact
     4801 tatgactata tggggcaaat atggttacgc cagtaagcat ctgcaattac atatctcttc
     4861 ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta aaggatttta
     4921 ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta aacaccaact
     4981 cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag aggacttctt
     5041 ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact tatcctgtca
     5101 agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta agccctattt
     5161 atttagataa ttttcgccat gatggagaaa ttaatatttt ttgcgctccc aacccaaagc
     5221 ctgtcactat ggggcgcgta tttcagaccg gcgtcgagcg tgtgctcttt ctgtttatga
     5281 atgattttat tgaacaattt ccaatgatca accttggcgc ccccatcaaa agagcgcata
     5341 caccacatat tgaacccctg cctccggatc accataccgc tgcagattac ttacgccagt
     5401 ttgatttgct tgtcctgaat tttatctctc gcggtaattt tgtcatactc ccccgattat
     5461 ggaataactc tgaggttcac agatggttcg tcaataaaga tcctaacttg atcaccgcca
     5521 ttctcgacat aacggacagc gaattaaaag aggatttatt gcaaagcctg atggattcat
     5581 taggttctaa caaacatgta caacccgaag tctgcatctg cttcttatcc cttttagcag
     5641 aacaagaatc tcctcatttt caggacttgt ttttattttt cgccaatatg ttattgcact
     5701 atcaccaatt tatgaatccc aatgaaagtg atctgaatga cgtgttgatg ccagcatcat
     5761 taagtgatga taaaattatc aaacatatgg cgcgcaggac cctcaaactg tttgtaaaaa
     5821 atgaaacgcc gccaaaagtt actcacgaag acctggtgaa aaacaggcct cgctcccctg
     5881 tcagaccacc tatacccgca accgccaaaa cgccagacct ccctgaacgt cattaaaccg
     5941 tgatgttacc gactctctga ctcggaacag aatcagcgtc agagaaacgg aaaacgcgat
     6001 ccagatcaca aatgcattgt attcacatca ttaaccgttt taagatcatt tcatcacttt
     6061 ttcgcaactc acccgataat ctgttatgac aacaaacact gtttcccgca aagtggcgtg
     6121 gctacgggtc gttacgctgg cagtcgccgc cttcatcttc aacaccaccg aatttgtccc
     6181 tgttggcctg ctctctgaca ttgcgcaaag ttttcacatg caaaccgctc aggtcggcat
     6241 catgttgacc atttacgcat gggtagtagc gctaatgtca ttgcctttta tgttaatgac
     6301 cagtcaggtt gaacggcgca aattactgat ctgcctgttt gtggtgttta ttgccagcca
     6361 cgtactgtcg tttttatcgt ggagctttac cgttctggtg atcagtcgca ttggtgtggc
     6421 ttttgcacat gcgattttct ggtcgattac ggcgtctctg gcgatccgta tggctccggc
     6481 cgggaagcgg gcacaggcct tgagtttaat tgccaccggt acagcactgg cgatggtctt
     6541 aggtttaccg ctcgggcgca ttgtggggca gtatttcggc tggcgaatga ccttcttcgc
     6601 gattggtatt ggggcgcttg ttaccctttt gtgcctgatt aagttacttc ccttactgcc
     6661 tagtgagcat tccggttcat tgaaaagcct cccgctattg ttccgccgcc cggcattgat
     6721 gagcatttat ttgttaactg tggtggttgt caccgcccat tacacggcat acagctatat
     6781 cgagcctttt gtgcaaaaca ttgcgggatt cagcgccaac tttgccacgg cattactgtt
     6841 attactcggt ggtgcgggca ttattggcag cgtgattttc ggtaaactgg gtaatcaata
     6901 tgcgtctgcg ttggtaagta cggcgattgc gctgttgctg gtgtgtctgg cactgctgct
     6961 acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt attttctggg ggatcgcgat
     7021 gatgatcatc gggcttggta tgcaggttaa agtgctggcg ctggcaccag atgctaccga
     7081 cgtcgcgatg gcgctattct ccggcatatt taatattgga atcggggcgg gtgcgttggt
     7141 aggtaatcag gtgagtttgc actggtcaat gtcgatgatt ggttatgtgg gcgcggtgcc
     7201 tgcttttgct gcgttaatat ggtcaatcat tatatttcgc cgctggccag tgacactcga
     7261 agaacagacg caatagttga aaggcccatt cgggcctttt ttaatggtac gttttaatga
     7321 tttccaggat gccgttaata ataaactgca cacccataca taccagcagg aaccccatca
     7381 ggcgggagat cgcttcaatg ccacccttgc ccaccagccg cataattgcg ccggagctgc
     7441 gtaggcttcc ccacaaaata accgccacca ggaaaaagat cagcggcggc gcaaccatca
     7501 gtacccaatc agcgaaggtt gaactctgac gcactgtgga cgccgagcta ataatcatcg
     7561 ctatggttcc cggaccggcg gtacttggca ttgccagtgg tacaaaggca atattggcac
     7621 tgggttcatc ttccagctct tccgacttgc ttttcgcctc cggtgaatca atcgctttct
     7681 gttgcggaaa aagcatccga aaaccgataa acgcgacgat taagccgcct gcaattcgca
     7741 gaccgggaat cgaaatgcca aatgtatcca tcaccagttg cccagcgtaa tacgccacca
     7801 tcatgatggc aaatacgtac accgaggcca tcaacgactg acgattacgt tcggcactgt
     7861 tcatgttgcc tgccaggcca agaaatagcg cgacagttgt taatgggtta gctaacggca
     7921 gcaacaccac cagccccagg ccaattgctt taaacaaatc taacattggt ggttgttatc
     7981 ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa caaggataaa gtgtcactct
     8041 ttagctaacc ttgcatcgca ttgaccaaaa cttgaaccga tttagcaaaa cgtggcatcg
     8101 gtcaattcat tcatttgact tatacttgcc tgggcaatat taccccctac aactaattac
     8161 ttgccaaggc aactaatgtg aaaagtacca gcgatctgtt caatgaaatt attccattgg
     8221 gtcgcttaat ccatatggtt aatcagaaga aagatcgtct gcttaacgag tatctgtctc
     8281 cgctggatat taccgcggca cagtttaagg tgctctgctc tatccgctgc gcggcgtgta
     8341 ttactccggt tgaactgaaa aaagtgttgt cggtcgacct gggagcactg acccgtatgc
     8401 tggatcgcct ggtctgtaaa ggctgggttg aaaggttgcc gaacccgaat gataagcgcg
     8461 gcgtactggt aaaacttacc accagcggcg cggcaatatg cgaacaatgc catcaattag
     8521 ttggccagga cctgcatcaa gaattaacaa aaaacctgac ggcggacgaa gttgcaacac
     8581 ttgagcattt gcttaagaaa gtcctgccgt aaacaaaaaa gaggtatgac gatgtccaga
     8641 cgcaatactg acgctattac cattcatagc attttggact ggatcgagga caacctggaa
     8701 tcgccactgt cactggagaa agtgtcagag cgttcgggtt actccaaatg gcacctgcaa
     8761 cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgcag ccgtaagatg
     8821 acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct ggcagaacga
     8881 tatggcttcg agtcgcaaca aactctgacc cgaaccttca aaaattactt tgatgttccg
     8941 ccgcataaat accggatgac caatatgcag ggcgaatcgc gctttttaca tccattaaat
     9001 cattacaaca gctagttgat aacgtgacaa cgtcactgag gcaatcatga aaccactttc
     9061 atccgcaata gcagctgcgc ttattctctt ttccgcgcag agcgttgcgg aacaaaccac
     9121 gcagccagtt gttacttctt gtgccaatgt cgtggttgtt cccccatcgc aggaacaacc
     9181 accgtttgat ttaaatcaca tgggtactgg cagtgataag tcggatgcgc tcggcgtgcc
     9241 ctattataat caacacgcta tgtagtttgt tctggccccg acatctcggg gcttattaac
     9301 ttcccacctt taccgcttta cgccaccgca agccaaatac attgatatac agcccggtca
     9361 taatgagcac cgcacctaaa aattgcagac ccgttaagcg ttcatccaac aatagtgccg
     9421 cacttgccag tccgactacg ggcaccagta acgataacgg tgcaacccgc caggtttcat
     9481 agcgtcccag taacgtcccc cagatcccat aaccaacaat tgtcgccaca aacgccagat
     9541 acatcagcga caagatggtg gtcatatcaa tagtaaccag actgtgaatc atggatgcgg
     9601 aaccatcgag aatcagcgag acaacaaaga agggaatgat tgggattaaa gcgctccaga
     9661 ttaccagcga catcaccgcc ggacgcgttg agtgcgacat gatcttttta ttgaagatgt
     9721 tgccacacgc ccaactaaat gctgcagcca gggtcaacat aaagccgagc atcgccacat
     9781 gctgaccgtt cagactatct tcgattaaca ccagtacgcc aaaaatcgct aaggcgatcc
     9841 ccgccaattg tttgccatgc agtcgctccc cgaaagtaaa cgcgccaagc acgatagtaa
     9901 aaaacgcctg tgcctgtaac accagcgaag ccagtccagc aggcatacca aagttaatgg
     9961 cgcagaacag aaaggcaaac tgcgcaaaac tgatggttaa tccatacccc aagagcaaat
    10021 tcagtggtac tttcggtcgt gcaacaaaaa agatggccgg aaaagcgacc agcataaagc
    10081 gcaaaccggc cagcatcagc ggtggcatgt tatgaagccc cactttgatg accacaaaat
    10141 ttagccccca tacgaccact accagtagcg ccaacacccc atcttttcgc gacattctac
    10201 cgcctctgaa tttcatcttt tgtaagcaat caacttagct gaatttactt ttctttaaca
    10261 gttgattcgt tagtcgccgg ttacgacggc attaatgcgc aaataagtcg ctatacttcg
    10321 gatttttgcc atgcgtccga tggttaataa caacgacgag gcaagaaggg cgctggtaga
    10381 gcgtcgtagg gataagttca tttgtctgtc cggcagtgcg tttcgttatg ttttatttta
    10441 gagatgtaaa gaaatagcat ggctacgctg ccatttatga ccatttactt gagtcgccag
    10501 tacagcctga gtgtcgatct aatcggttat gcgatgacaa ttgcgctcac tattggcgtc
    10561 gtttttagcc tcggttttgg tatcctggcg gataagttcg acaagaaacg ctatatgtta
    10621 ctggcaatta ccgccttcgc cagcggtttt attgccattc ctttagtgaa taacgtgacg
    10681 ctggttgtgc tcttttttgc cctcattaac tgcgcctatt ctgtttttgc taccgtgctg
    10741 aaagcctggt ttgccgacaa tctttcgtcc accagcaaaa cgaaaatctt ctcaatcaac
    10801 tacaccatgc taaacattgg ctggaccatc ggtccgccgc tcggcacgct gttggtgatg
    10861 cagagcatca atctgccctt ttggctggca gctatctgtt ccgcgtttcc catgcttttc
    10921 attcaaatat gggtaaagcg cagcgagaaa atcatcgcca cggaaacagg cagtgtctgg
    10981 tcgccgaaag ttttattaca agataaagca ctgttgtggt ttacctgctc tggttttctg
    11041 gcttcttttg taagcggcgc atttgcttca tgcatttcac aatatgtgat ggtgattgct
    11101 gatggggatt ttgccgaaaa ggtggtcgcg gttgttcttc cggtgaatgc tgccatggtg
    11161 gttacgttgc aatattccgt gggccgccga cttaacccag ctaacatccg cgcgctgatg
    11221 acagcaggca ccctctgttt cgtcatcgga ctggtcggtt ttattttttc cggcaacaac
    11281 ctgctattgt ggggtatgtc agctgcggta tttactgtcg gtgaaatcat ttatgcgccg
    11341 ggcgagtata tgttgattga ccatattgcg ccgccaggaa tgaaagccag ctatttttcc
    11401 gcccagtctt taggctggct tggtgccgcg attaacccat tagtgagtgg cgtagtgcta
    11461 acca
//
LOCUS       DAJTYM010000111        11397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTYM010000111 DAJTYM010000000
VERSION     DAJTYM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            317..1957
                     /gene="trg"
                     /locus_tag="OO575_003283"
     CDS             317..1957
                     /gene="trg"
                     /locus_tag="OO575_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6666135.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(1995..2918)
                     /locus_tag="OO575_003284"
     CDS             complement(1995..2918)
                     /locus_tag="OO575_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6666136.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3135..4478
                     /locus_tag="OO575_003285"
     CDS             3135..4478
                     /locus_tag="OO575_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6666137.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4703..6358
                     /gene="opgD"
                     /locus_tag="OO575_003286"
                     /gene_synonym="mdoD"
     CDS             4703..6358
                     /gene="opgD"
                     /locus_tag="OO575_003286"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6666138.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6498..6722
                     /locus_tag="OO575_003287"
     CDS             6498..6722
                     /locus_tag="OO575_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6666139.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6785..7321
                     /gene="rimL"
                     /locus_tag="OO575_003288"
     CDS             6785..7321
                     /gene="rimL"
                     /locus_tag="OO575_003288"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6666140.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7316..8296)
                     /locus_tag="OO575_003289"
     CDS             complement(7316..8296)
                     /locus_tag="OO575_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666141.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8420..9412
                     /gene="tehA"
                     /locus_tag="OO575_003290"
     CDS             8420..9412
                     /gene="tehA"
                     /locus_tag="OO575_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6666142.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9409..10002
                     /gene="tehB"
                     /locus_tag="OO575_003291"
     CDS             9409..10002
                     /gene="tehB"
                     /locus_tag="OO575_003291"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6666143.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10305..10961
                     /locus_tag="OO575_003292"
     CDS             10305..10961
                     /locus_tag="OO575_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6666144.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10979..11397)
                     /gene="tnpA"
                     /locus_tag="OO575_003293"
                     /pseudo
     CDS             complement(10979..11397)
                     /gene="tnpA"
                     /locus_tag="OO575_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2939 a         2665 c         2864 g         2929 t
ORIGIN      
        1 atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa agtctcaggc
       61 acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca cgaaattgcg
      121 tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc gttactctct
      181 tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga cgcaaagagg
      241 tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga gtaaacctgg
      301 acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt tgcatcacat
      361 caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat tcgcattaag
      421 ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg atgttactgc
      481 ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt cagcccggat
      541 caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa tgaagcgaaa
      601 tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg cttatcagaa
      661 tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc aacgctttca
      721 ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca tgtttgaagc
      781 gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata ccgatatttt
      841 gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac tggcccatca
      901 gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc tggtcatgac
      961 gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac atgccgcaca
     1021 acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg cgggtcgtaa
     1081 tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg ggatgacagt
     1141 agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa tttcagctgg
     1201 caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac aaactgccgc
     1261 cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc atcatgccag
     1321 caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg tttccggtgt
     1381 agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga tcaccgccgt
     1441 catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg ttgaagccgc
     1501 gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac ggacactcgc
     1561 aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat cagtcaggtt
     1621 aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca ctattgttga
     1681 tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg atgaacaaag
     1741 tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga cgcaacagaa
     1801 tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac aggctgcacg
     1861 attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg cagaacctcg
     1921 cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga gggatcgaca
     1981 gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat caactggcaa
     2041 ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc tgcaccatgt
     2101 cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac agcgccagaa
     2161 gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga agtagacagc
     2221 gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc atctgaatgc
     2281 tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac ccggcttagc
     2341 gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa cgattcaaga
     2401 aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg accaatgccg
     2461 atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat tgtcgcaact
     2521 tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg taatatcccc
     2581 agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc ttctttacga
     2641 tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg cgttaaaaat
     2701 tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa gcgagcgccc
     2761 gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa attaagggtt
     2821 tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg aaggtggcgc
     2881 aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa aaacgaagcg
     2941 gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat attattaaca
     3001 attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg cgagatcaca
     3061 gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat tactgaccac
     3121 tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa cagttttcgc
     3181 aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg ctggaactgg
     3241 tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa aaaatggtaa
     3301 atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc gttgggactg
     3361 cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac ccggtgagct
     3421 attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg cccattgatg
     3481 atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc cttgagctta
     3541 tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc gatatcttca
     3601 ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt aacgaaacac
     3661 aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag ttagcaacgg
     3721 tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct gatgtggtct
     3781 gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt gaccgggtgc
     3841 agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg ccgccgcgcc
     3901 gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag acggtgttgt
     3961 ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag cagcgtggcg
     4021 tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac gctggaaccg
     4081 ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact tttcctgaca
     4141 gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg acgccttcgg
     4201 gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta attgaacgtg
     4261 gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc gcggcgggga
     4321 tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat gccagtcgcg
     4381 aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt taccaggaag
     4441 cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc cgcctttgca
     4501 aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa aaaaatgccg
     4561 atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt ctagagtgag
     4621 cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc attcacaaca
     4681 ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt caatggctat
     4741 ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag cattcgcggc
     4801 agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc tacagtcaat
     4861 ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac ctgacacgct
     4921 ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa aatcgctctg
     4981 gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa tgggattccg
     5041 tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg aaattcactt
     5101 tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat tagaagggca
     5161 aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg cgcgccgtga
     5221 tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat atcaatacgg
     5281 tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag agttccccga
     5341 ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta ccgtttatgc
     5401 gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt gtgagaaaag
     5461 tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta aacagctggg
     5521 catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga tgtgcgatac
     5581 aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca acggcgagtg
     5641 gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca ccgacaacaa
     5701 cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc aggacattat
     5761 gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt ggggtaaggg
     5821 caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca ttgtctgctt
     5881 ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt atcgtctgta
     5941 ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga cgcgtaccgg
     6001 catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa aatgggcgcg
     6061 tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa aaggcattga
     6121 gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct atattgaacc
     6181 catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca ctgatccggt
     6241 cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat ggctgtatca
     6301 gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga tgagttaatc
     6361 gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat cccgctcgca
     6421 tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac cgtgtcagcc
     6481 actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt ctgaaaaacg
     6541 aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat gaaattgcca
     6601 gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg aaaaaacaaa
     6661 agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc aaagaggtgt
     6721 aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat tgactggaga
     6781 taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg ttgcggaaag
     6841 tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac agcagtcgct
     6901 aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc agggtaatgt
     6961 gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag atgaacttat
     7021 cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa tcggctactg
     7081 gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga cattgattca
     7141 tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg tggacaatcc
     7201 gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc tgaaacaggc
     7261 tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta tcgattcata
     7321 ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact tttggaccac
     7381 ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt aactccacat
     7441 ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca acgtgctcgt
     7501 cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca tgatgtttta
     7561 acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa tgaatcgtcg
     7621 gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact ttagcgcaat
     7681 cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca aaaacttcgg
     7741 cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt gcctggtgga
     7801 caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt tgatggtcgg
     7861 gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg tgaccaaata
     7921 ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt atatatgcac
     7981 cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc tctccccaca
     8041 gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg aacgcaatag
     8101 cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg cgatcgatcc
     8161 agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata atctgccgta
     8221 ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt tcttcactta
     8281 ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta gcaaattctg
     8341 cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac tttataaatt
     8401 aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg caggctactt
     8461 tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca gccaggtttg
     8521 gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca tctggggatt
     8581 attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc tggcggaagt
     8641 tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga tgctggtggc
     8701 gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt ttggtgtcgt
     8761 ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat ctcaccctga
     8821 agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta tcagcgcaat
     8881 ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg caggcgtttt
     8941 ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg gagaattacc
     9001 cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg cctgtagtgc
     9061 ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt tcggttatgg
     9121 actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc agccatttaa
     9181 tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg gtttgcatct
     9241 ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt ttatctttac
     9301 caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc agggaaaatt
     9361 gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat gatcattcgt
     9421 gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga agtactggaa
     9481 gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaac
     9541 agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa tgccatgagt
     9601 atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca cacccgggtc
     9661 gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc gactgtggtg
     9721 ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca acgttgcact
     9781 aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta tccatgtacc
     9841 gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga aggctgggag
     9901 agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt
     9961 attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa gcacaaaacg
    10021 cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag catatttttt
    10081 atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt aacttactga
    10141 aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt cgtctgaaaa
    10201 ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag cggcgctttt
    10261 tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt accacatcat
    10321 ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc tctaaaatca
    10381 cccagccaga taaatataac aattactctg atttaaaaga aacaacctca gcaacaggta
    10441 aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac agcatcgtct
    10501 ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg cagaaagttc
    10561 tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg cagcgtaaac
    10621 cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt accggtgtag
    10681 ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta gtggttgcgg
    10741 ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt gaaggtgagc
    10801 tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc gaaggtaaag
    10861 acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt attgatgaca
    10921 tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg cgccatcgtt
    10981 agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg ggatataagg
    11041 cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg atgacggata
    11101 ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg cctttgtagt
    11161 aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat acgcctttga
    11221 ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac aaatggacgt
    11281 gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat acatttgaaa
    11341 agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg tatctgg
//
LOCUS       DAJTYM010000112        11245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTYM010000112 DAJTYM010000000
VERSION     DAJTYM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..599)
                     /gene="hdhA"
                     /locus_tag="OO575_003294"
     CDS             complement(<1..599)
                     /gene="hdhA"
                     /locus_tag="OO575_003294"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6666145.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADSAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSV"
     gene            complement(711..1739)
                     /gene="malI"
                     /locus_tag="OO575_003295"
     CDS             complement(711..1739)
                     /gene="malI"
                     /locus_tag="OO575_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6666146.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1914..3506
                     /gene="malX"
                     /locus_tag="OO575_003296"
     CDS             1914..3506
                     /gene="malX"
                     /locus_tag="OO575_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6666147.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3516..4688
                     /gene="malY"
                     /locus_tag="OO575_003297"
     CDS             3516..4688
                     /gene="malY"
                     /locus_tag="OO575_003297"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6666148.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4792..5793
                     /gene="add"
                     /locus_tag="OO575_003298"
     CDS             4792..5793
                     /gene="add"
                     /locus_tag="OO575_003298"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6666149.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5829..6869)
                     /locus_tag="OO575_003299"
     CDS             complement(5829..6869)
                     /locus_tag="OO575_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6666150.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7112..7237
                     /gene="blr"
                     /locus_tag="OO575_003300"
     CDS             7112..7237
                     /gene="blr"
                     /locus_tag="OO575_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6666151.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7510..7725
                     /gene="ydgT"
                     /locus_tag="OO575_003301"
     CDS             7510..7725
                     /gene="ydgT"
                     /locus_tag="OO575_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6666152.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7811..8251
                     /locus_tag="OO575_003302"
     CDS             7811..8251
                     /locus_tag="OO575_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6666153.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8328..8909
                     /gene="rsxA"
                     /locus_tag="OO575_003303"
     CDS             8328..8909
                     /gene="rsxA"
                     /locus_tag="OO575_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6666154.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8909..9487
                     /gene="rsxB"
                     /locus_tag="OO575_003304"
     CDS             8909..9487
                     /gene="rsxB"
                     /locus_tag="OO575_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6666155.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9480..>11245
                     /gene="rsxC"
                     /locus_tag="OO575_003305"
     CDS             9480..>11245
                     /gene="rsxC"
                     /locus_tag="OO575_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6666156.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         2619 a         2897 c         2835 g         2894 t
ORIGIN      
        1 acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac ccgaatattt
       61 ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc tttagatgat
      121 gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat ggtcagaata
      181 acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg gaaaaaagaa
      241 aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa cggtttagga
      301 ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact gacggcagag
      361 tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc aaatgcctga
      421 ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt aatatcactg
      481 accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc aatacctgca
      541 cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga attaaacaca
      601 taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca cattttttat
      661 cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga ttatttcgcc
      721 gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg ggtgattttt
      781 tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct ggcccaggta
      841 acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag cgaaacctgt
      901 tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt cagcaaacca
      961 aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat ggtcgggtta
     1021 tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt gcactccaac
     1081 acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata gccccccacc
     1141 cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg ctgatgccca
     1201 ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg gcgaaccgta
     1261 tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc tttttcttct
     1321 gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac accatcgaca
     1381 ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt accgccgtga
     1441 agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc cgtcaattcg
     1501 gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct ttgcccgccg
     1561 cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc ggcgttaacg
     1621 cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga aacggtactt
     1681 accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc ggtagccatg
     1741 cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta tttatcttat
     1801 acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat aaaacgtttt
     1861 atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt tttatgacgg
     1921 cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc aaaaccttca
     1981 tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt agttctctta
     2041 gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg caggctatct
     2101 ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc atgttctgta
     2161 tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc gctggcttcg
     2221 tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa ggcattctgc
     2281 caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt gggatccagt
     2341 cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg atgctgcatg
     2401 agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt acgcgcttcg
     2461 taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca ttagtctggc
     2521 cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg ggtgatttcg
     2581 gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg catcacattc
     2641 tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc ggtcaaaccg
     2701 tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact cacggttttt
     2761 ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc ggcggtctac
     2821 caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat aaaattaaag
     2881 gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa ccgctggaat
     2941 tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta accggcctcg
     3001 gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc aatatcatcg
     3061 acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg gtgccagtgg
     3121 tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc acccgcttca
     3181 atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa gccgttgccg
     3241 gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta ggcggtgccg
     3301 acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg gaagatatgt
     3361 cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta caacttaatc
     3421 aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat gaaatggccg
     3481 gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca aaggtcgtgg
     3541 atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc ggcactgctg
     3601 acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc attatcgagg
     3661 cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa aacgatgagt
     3721 ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt gatacgcaga
     3781 cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt cagtggtctg
     3841 aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac aaggccattg
     3901 aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac ggctggtttt
     3961 gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt atgctcctgt
     4021 gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag atcatggctg
     4081 acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg gatatggtct
     4141 ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg gcgttgctga
     4201 catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg attatagaaa
     4261 atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg ctttcttccc
     4321 cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca ccgtggctgg
     4381 atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa atgaacgccg
     4441 cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg cttgatctac
     4501 gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa gaaaaagtcg
     4561 cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt ctcaatgccg
     4621 gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc atccgcgctg
     4681 ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat ttttaccctg
     4741 ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac catgattgat
     4801 accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg tccccagacc
     4861 attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct tgaaacactg
     4921 attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct gaccaaactt
     4981 gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc atttgaaaac
     5041 attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc accaggctac
     5101 atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat cgatggcgta
     5161 cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat gagccgaacc
     5221 ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg tgaccagatt
     5281 accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt cctttctcac
     5341 ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga agctgccggg
     5401 ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg acatggcgta
     5461 aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat tggtattgaa
     5521 tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc acatccgctg
     5581 aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc cggcgtacag
     5641 ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt atcccgtgag
     5701 caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc agaggaaaaa
     5761 cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca aattgcacca
     5821 tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat gcccagctca
     5881 atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc atcgccattt
     5941 aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt caacagcgtt
     6001 tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat atcgtagccc
     6061 cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg atcgaggcca
     6121 tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc agcggctgcc
     6181 agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc gtggaaataa
     6241 tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct gaccggtaaa
     6301 ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca gataccgctg
     6361 cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg gtcaaaatga
     6421 gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt tagcgtcaag
     6481 aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag gctttttgcc
     6541 agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc aacgaccaca
     6601 tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt gggtgtagga
     6661 atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga gacaaccgta
     6721 accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac cgccagttcc
     6781 agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata accataccca
     6841 atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt atgatggcta
     6901 tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa taagggatgt
     6961 tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa atatcgcaaa
     7021 aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc ccctgaagtt
     7081 aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat taacaggttg
     7141 gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg acaactatca
     7201 gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt ggagcgacat
     7261 gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat ttattatctt
     7321 gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt cagagcagtt
     7381 tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca caagcgttca
     7441 cttttggtga aattttatca tcactaataa tacctacccc tttggtatta attattttat
     7501 ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag ttcactcgaa
     7561 agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca ggaactgatc
     7621 aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg gcgtttgttt
     7681 gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa gtagcgatcg
     7741 gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc gtcgctattc
     7801 tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc ttggcccgtt
     7861 ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt ataccgctgc
     7921 gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga cacaaatctt
     7981 atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt ggttgaccat
     8041 cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc tgcttatttc
     8101 cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca tagccgttcg
     8161 ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca aacgctcgtc
     8221 gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg ttgtcgcctg
     8281 ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg actgactacc
     8341 tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag tttctcggtc
     8401 tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg gggctggcaa
     8461 caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg tggattttga
     8521 tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt gctgtggtcg
     8581 tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt ctgctgggga
     8641 tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg ctgaatatca
     8701 atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct gtcggtttct
     8761 cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct gatgtcccgg
     8821 caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct ctggccttta
     8881 tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg ttgccgccgt
     8941 gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc gttttgcggt
     9001 ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc agtgtggtca
     9061 gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg gtgaaaaaat
     9121 caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt tgcttaatgt
     9181 cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga tggtggcggt
     9241 tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc cggtagacgc
     9301 catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta cgggctgcaa
     9361 tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg cagaaacacc
     9421 tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg tggaacacca
     9481 tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac ggcggcatcc
     9541 atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta ctactggcgc
     9601 agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg tgcgttagcg
     9661 tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa atgctgcctg
     9721 ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg gctcatcctt
     9781 cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc tggatcccgc
     9841 gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag cgcatacatc
     9901 agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa ttgcagggtg
     9961 gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg tacattaccg
    10021 ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt cgcattcttg
    10081 cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa ccgcaggcga
    10141 tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg gtgattccaa
    10201 ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg aagcaggttc
    10261 cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc actgcttatg
    10321 cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta accctgactg
    10381 gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca gtgcgtcatt
    10441 tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg ggtggcccgc
    10501 taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc aactgtctgt
    10561 tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc atccggtgta
    10621 gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac tggttcagca
    10681 aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt gaatgtgggg
    10741 cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt caggaaaaag
    10801 ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa gcgcgtttcg
    10861 aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga cataagagcg
    10921 cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg gcgcgggtga
    10981 aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa cgcccggata
    11041 acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg aaacaggcag
    11101 aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact gccgttgaag
    11161 cagctatcgc ccgctccaaa gcgcgcaagc tggaacagca acaggctaat gcggaaccag
    11221 aagaacaggt cgatccgcgc aaagc
//
LOCUS       DAJTYM010000113        11214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTYM010000113 DAJTYM010000000
VERSION     DAJTYM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            554..2806
                     /locus_tag="OO575_003306"
     CDS             554..2806
                     /locus_tag="OO575_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666157.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3142..4074
                     /gene="csiD"
                     /locus_tag="OO575_003307"
     CDS             3142..4074
                     /gene="csiD"
                     /locus_tag="OO575_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6666158.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4094..5362
                     /gene="lhgO"
                     /locus_tag="OO575_003308"
     CDS             4094..5362
                     /gene="lhgO"
                     /locus_tag="OO575_003308"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6666159.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5385..6833
                     /gene="gabD"
                     /locus_tag="OO575_003309"
                     /pseudo
     CDS             5385..6833
                     /gene="gabD"
                     /locus_tag="OO575_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6841..8127
                     /gene="gabT"
                     /locus_tag="OO575_003310"
                     /pseudo
     CDS             6841..8127
                     /gene="gabT"
                     /locus_tag="OO575_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8365..9765
                     /gene="gabP"
                     /locus_tag="OO575_003311"
     CDS             8365..9765
                     /gene="gabP"
                     /locus_tag="OO575_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6666160.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9786..10448
                     /gene="csiR"
                     /locus_tag="OO575_003312"
     CDS             9786..10448
                     /gene="csiR"
                     /locus_tag="OO575_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6666161.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10449..10898)
                     /gene="kbp"
                     /locus_tag="OO575_003313"
     CDS             complement(10449..10898)
                     /gene="kbp"
                     /locus_tag="OO575_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6666162.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10982..11140)
                     /locus_tag="OO575_003314"
     CDS             complement(10982..11140)
                     /locus_tag="OO575_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6666163.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2884 a         2746 c         2809 g         2775 t
ORIGIN      
        1 tatgcaataa caatacttat aaaccacgca ataaacatga tgatcttatg atgtaaataa
       61 cagattttta tgcgttccca ttcaccttgg gtcgtgatta cacgcgacat aaaacccgac
      121 accgcctcca ttcgcaaagt cgatactcgc agtcaacaag caaatgttaa taattagcac
      181 tatctatagt tatcatcgat tcaatgatac tttgtaatga ttttgtatct aataatataa
      241 ctttattaca ttagctgaag agttttcgca tcattatgat atctgttact tttcactcca
      301 taaaaataaa cttcatatag caatatattc tttcatagat cttattctgc taatcattag
      361 tttcgtatgc gcgatttttg acagttgcct ctccagacca catcgataat taaaaaacag
      421 atttaaacat tatccttttc catatagata ttggataaaa ataggacatc tgtttgcaat
      481 tactttcaca acaattaaac atttttatgt tttcgtatac atcatattac tctaccatta
      541 gaggaacgtt attatgtttt ctataaaacc aggacctgga aatttaccta tcgacaaccc
      601 cacattgtta tcatggaaca ttactgatgg ggatctaaat tccaaattta atacattaga
      661 atatctaaac tgtataacaa atattattaa tgcttgtgga gtttatcctc aggatttaaa
      721 agacagagaa attatatcaa cttttcacgc agaaaaagtc attaatgatc tgttaaaaaa
      781 cgattataaa atttcccttt ctccagatac aacttaccga gagctgaata aagctgcaca
      841 gagtagcatt acagcgccag acaggatagg agaagggaaa acatgggttt atcaacgaga
      901 tacaatggtt gaaagaggtg ataacagtgg tgttcatcag tatggtccag ctgaacattt
      961 cactcacatt atatctgaca aaccgtcccc aaaagataag tatgttgcat atgctattaa
     1021 cattcctgac tatgagctag cagccgatgt atataatatt aacgtgacgt caccttccgg
     1081 acagcaagaa acatttaaaa tactaatcaa tccagaacat ctacggcaaa cacttgagcg
     1141 taaatctctt actgctgttc agaaatcaca atgtgaaatc atcaccccca aaaaacctgg
     1201 cgaagcgatt cttcatgctt ttaatgccac ctaccagcaa atcagggaaa atatgtctga
     1261 gtttgcacgt tcccattatg ggtatataca aatccctcca gtgacaactt tccgcgccga
     1321 cggaccagaa actcccgaag aagaaaaagg ttactggttt cacgcttatc aacccgaaga
     1381 tctttgtacc atccataatc caatggggga tttgcaggat tttatcgcat tggttaaaga
     1441 tgctaaaaaa tttggtatcg atatcattcc tgattatacc tttaacttta tgggaatcgg
     1501 gggaagtggt aaaaatgacc tggattatcc ctctgctgat atacgagcga agatcagtaa
     1561 agatatagaa agtggtatcc ctggctattg gcaaggtcag gttttgattc cattcattaa
     1621 agatccagta acaaaagaac gtaaacaaat ccatccagaa gatatacatc tcactgcaaa
     1681 agacttcgaa gcaagtaaag ataacatctc taaggatgaa tggaaaaacc tccatgcatt
     1741 aaaagaaaag agtttaaatg gaatgcctaa aacaaccccc aaaagtgacc aggttattat
     1801 gttgcaaaat caatacgttc gtgaaatgcg aaaatatggc gtacgaggtt tacgttatga
     1861 tgcagcaaaa cactcaaaac atgaacaaat agaaagatca ataaccccac cgcttaaaaa
     1921 ttataatgag cgattacaca atactaactt atttaaccca aaatatcatg aaaaaaccgt
     1981 tatgaattac atggaatatc tggtaacttg tcagttgaat gaagaacaaa tgtcatcgct
     2041 gctttatgaa agagatgatt taagcgccat tgatttttca ttgctcatga agacgataaa
     2101 agccttttca ttcgggggag atctcctaac ccttgcatca aaaccgggtt ccacaatctc
     2161 aagcatccca tcagaaagac ggatattgat taacattaac cacgattttc ctaacaatgg
     2221 caatcttttc aatgactttc tatttaacca tcaacaagat gaacaattag caatggcata
     2281 tatggccgct ctcccgttca gcaggccttt agtttactgg gatggccagg tattaaaatc
     2341 aacgactgaa attaaaaatt atgatgggcc cacgcgtgtc ggtggtgagg cgtggcttaa
     2401 taaaggttgc tctacctatc agcagctcta caatgaattc cacgcattat atatagataa
     2461 agcaggaata tggagcgcat ttgagggtgt atttgcaact aaaaacgttc tggcctttag
     2521 tcgtggggat tctgtgaaca ttaatcactc tcctcatgat ggactagtta taataaataa
     2581 aggaaacgaa gaagttgaag gtacctggcc taacaaattg caacctggaa tatataaaaa
     2641 catggggagt aatagcgtta acattattat taataatacc cgaaaaatta tccccccttg
     2701 taaagtattt acgcttagag gcggatctct aaatatcaat attcctaggc gtagcgctct
     2761 tcttttaggg aaaacaggag aaccgccgaa ctatctctat ttataattta atgttatatc
     2821 tgccccgata aaacggggca gataatatgt ttagtttact aacggtcatt ttgtagtgaa
     2881 gccatttact gttttttatc gaccagataa tctgttctct aatgttaact ccccctaacc
     2941 tgttgcttta gttattcatt tcctgtctca ctttgcctta ataccctacg ttaaatgtta
     3001 ctaatttgtt gcttttgatc acaataagaa agcaatatgt cgcttttgtg cgcatttttc
     3061 agaaatgtag atatttttag attatggcta cgaaatgagc atcgccatgt caccctacat
     3121 ctcataagag gatcgcttct gatgaatgca ctgaccgccg tacaaaataa cgctgtcgat
     3181 tcaggccagg actatagcgg attcactctc atcccgtcgg cgcaatcccc gcgtctgctg
     3241 gaactcacct tcaccgaaca gacgaccaaa cagtttctcg aacaggttgc cgaatggccc
     3301 gtgcaggcgc tggagtacaa atcgtttctg cgttttcggg taggcaaaat tcttgacgat
     3361 ctgtgtgcga atcagctgca accattgctg ttgaagaccc tgctaaaccg tgctgaaggt
     3421 gcgctgttga tcaatgcggt aggtatcgat gatgtggcgc aggcagatga gatggtgaag
     3481 ctggcaacgg cggtagcgca tctgattggt cgctcaaatt tcgatgcgat gagcggtcag
     3541 tattacgcgc gtttcgtggt gaaaaatgtc gataactcag acagctatct gcgtcagccg
     3601 caccgcgtga tggaactgca caacgacggc acgtatgtcg aagagatcac cgattacgtg
     3661 ctgatgatga aaatcgatga ctgggaacat ctggaccact atttccgcca tccactggcg
     3721 cgtcgcccga tgcgctttgc cgcgccgccg agcaaaaacg tcagcaaaga tgttttccat
     3781 ccagtgttcg acgttgatca acagggtcgc ccggtgatgc gctatatcga ccagttcgtc
     3841 cagccaaaag attttgaaga aggcgtgtgg ttgagcgagc tttccgatgc cattgaaacc
     3901 agcaaaggca ttctttctgt acccgttccc gttggcaaat tcctgttgat aaacaacctg
     3961 ttctggctgc acggtcgcga ccgctttact ccgcacccgg atctgcgccg tgaactgatg
     4021 cgtcagcgtg gctatttcgc ttacgccact caccactacc agacgcatca gtaagcgcaa
     4081 aggaattgag cggatgtatg attttgtgat tattggcggc ggcatcatcg gcatgtcgac
     4141 cgccatgcaa ctgattgatg tttatccgga tgcccgcatt gcgttgctgg aaaaagagtc
     4201 cggcccggcc tgtcaccaga cgggccacaa cagcggcgtg atccatgccg gggtctatta
     4261 cacgcccggc agcctgaagg cgcagttttg cctggcgggg aaccgtgcca ccaaagcctt
     4321 ttgcgatcaa aacggcattc gctatgacaa ctgcggcaag atgctggtcg ccacgtctca
     4381 gctcgaaatg gaacggatgc gcgccttatg ggaacgcaca gcggcgaacg gtatcgagcg
     4441 cgagtggata aacgccgtgg aactgcgcga gcgcgaaccg aatatcaccg ggcttggtgg
     4501 catttttgtg ccgtccagcg gcattgtcag ctaccgcgaa gtgacggcgg caatggcaaa
     4561 aatcttccag gccagaggcg gcgagattat ttataacgcc gaagtcagcg ccctcagtga
     4621 gcataaaaac ggcgtggtga tacgcacccg tcagggcggt gaatatgaag catcgacgct
     4681 gattagctgt tccgggctga tggctgaccg gctggtgaaa atgctcggcc tcgaaccggg
     4741 ctttattatc tgcccgttcc gtggcgagta tttccgcctt gcgccggagc ataaccagat
     4801 tgttaaccac ctgatttacc ccattcccga ccccgcgatg ccatttttgg gcgtgcatct
     4861 cacccgcatg atcgacggca gcgtgaccgt cgggccaaac gcggtgctgg ctttcaaacg
     4921 cgaaggctat cgcaagcgcg acttctcatt tagtgacacg ctggagattt tgagctcgtc
     4981 ggggattcgc cgggtgctgc aaaaccatct acgctcagga ctgggcgaga tgaaaaactc
     5041 gctgtgcaaa agcggctatc tgcggctggt gcaaaagtat tgtccccggc tttcgttaag
     5101 cgatctccag ccctggcccg ccggtgtgcg ggcgcaggca gtatcgccgg acggcaagct
     5161 gattgacgat tttctgtttg tcaccacccc acgcacgatc cacacctgca atgcgccctc
     5221 cccggcagcg acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg tacaaacgct
     5281 gttggcaagc cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg tggatgcctt
     5341 acacgccgca tttaatcaat aacctttgaa aacaggacgt aacaatgaaa cttaacgaca
     5401 gtaacttatt ccgccagcag gcgttgatta acggggaatg gctggacgcc aacaatggtg
     5461 aagtcatcga cgtcaccaat ccggcgaacg gcgacaagct gggtagcgtg ccgaaaatgg
     5521 gcgcagatga aacccgcgcc gctatcgacg ccgccaaccg cgccctgccc gcctggcgtg
     5581 cgctcaccgc caaagaacgc gccaccattc tgcgcaactg gttcaatttg atgatgcagc
     5641 atcaggacga tttagcgcgc ctgatgaccc tcgaacaggg taaaccgctg gctgaagcga
     5701 aaggcgaaat cagctacgcc gcctccttca ttgagtggtt tgctgaagaa ggcaaacgta
     5761 tttatggcga caccattcct ggtcatcagg ccgataagcg cctgattgtt atcaagcagc
     5821 cgattggcgt caccgccgcc atcacgccgt ggaactttcc ggcggcgatg attacccgta
     5881 aagccggtcc ggcgctggcg gcaggctgca ccatggtgct gaagcccgcc agtcagacgc
     5941 cgttctctgc gctggcgctg gcggaactgg cgattcgcgc gggcattccg gctggcgtgt
     6001 ttaacgtggt caccggttcg gcaggcgtgg tcggtaatga actgaccagc aacccgctgg
     6061 tacgcaaact gtcgtttacc ggttagaccg agattggccg ccagttaatg gaacagtgcg
     6121 cgaaagacat caaaaaagtg tcgctggagc tgggcggcaa cgcgccgttt atcgtctttg
     6181 acgatgccga tctcgataaa gccgtggaag gcgcgctggc ctcgaaattc cgcaacgccg
     6241 ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat gaccgctttg
     6301 ccgaaaaatt acagcaggcg gggagcaaac tgcacatcgg cgacgggctg gagaaaggcg
     6361 tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa agtggaagag catattgccg
     6421 atgcgctgga gaaaggtgcg cgtgtggttt gcggcggtaa agcgcacgaa cgcggcggca
     6481 acttcttcca gccaaccatt ctggtggacg ttccggccaa cgccaaagtg tcgaaagaag
     6541 agacgttcgg ccccctcgcc ccactgttcc gttttaaaga tgaagccgat gtgattgcac
     6601 aagccaatga caccgagttt ggtcttgccg cctatttcta cgcccgtgat ttaagccgcg
     6661 tcttccgcgt cggcgaggcg ctggagtacg gcatcgtcgg catcaatacc ggcattattt
     6721 ccaatgaagt ggccccattc ggcggcatca aagcctcggg tctgggtcgt gaaggttcga
     6781 agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt taactggaga
     6841 atgcgaagga acagcaataa agagttaatg cagcgccgca gtcaggcgat tccccgtggc
     6901 gttgggcaaa ttcacccgat tttcgctgac cgcgcggaaa actgccgggt gtgggacgtt
     6961 taaggccgtg agtatcttga tttcgcgggc gggattgcgg tgctcaatac cgggcacctg
     7021 catccgaagg tggtggccgc ggtggaagcg cagttgaaaa aactgtcgca cacctgcttc
     7081 caggtgctgg cttacgagcc gtatctggag ctgtgcgaga ttatgaatca gaaggtgccg
     7141 ggcgatttcg ccaagaaaac gctgctggtt acgaccggtt ccgaagcggt ggaaaacgcg
     7201 gtaaaaatcg cccgcgccgc caccaaacgt agcggcacca tcgcttttag cggcgagtat
     7261 cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta ctctgcgggc
     7321 atggggctga tgccgggtca tgtttatcgc gcgctttatc cttgcccgct gcacggcata
     7381 agcgaggatg acgctatcgc cagcatccac cggatcttca aaaatgatgc cgcgccggaa
     7441 gatatcgccg ccatcatgat tgagccggtt cagggcgaag gcggtttcta cgccgcgtcg
     7501 ccagccttta tgcagcgttt acgcgctctg tgtgacgagc acgggatcat gctgattgcc
     7561 gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga gcagatgggc
     7621 gtggcgccgg atcttaccac ctttgcgaaa tcgatcgcag gcggtttccc gctggcgggc
     7681 gtcaccgggc gcgcggaagt gatggacgct gttgcaccgg gcggtctggg cggcacctat
     7741 gccggtaacc cgattgcctg cgtggctgcg ctggaagtgt tgaaggtgtt cgagcaggaa
     7801 aatctgctgc aaaaagccaa cgatctgggg cagaagctaa aagacggatt gttggcgata
     7861 gccgaaaaac acccggagat cggcgacgta cgcgggctgg gatcgatgat tgccatcgag
     7921 ctgtttgaag atggcgatca cagcaaaccg gacgcaaaac tcaccgccga gatcgtggca
     7981 cgcgcccgcg ataagggcct gattcttctc tcctgcggcc cgtattacaa cgtgctgcgc
     8041 atccttgtac cactcaccat tgaagacgct cagatccgtc agggtctgga gattatcggt
     8101 cagtgttttg atgaggcgaa gcagtagcac cgtccccatg caggaggcga cgctgcgcgt
     8161 cttgtccggc ctacgagaat ccaagtcgga taaggcgttt acgccgcatc cgacaatctg
     8221 tacgtgaaca ggaataaatc tatgtcggcc ggataaggcg gagccgctct ccggcaaaaa
     8281 gaatcaataa caattaaacg cgtgacccga cgcgggaaat gtcggggcgc tctcccaagt
     8341 gacacacttt cgagaggatt caggatgggg caatcatcgc aaccacacga gttaggcggc
     8401 gggctgaaat cacgccacgt caccatgttg tctattgccg gtgttatcgg cgcaagtctg
     8461 tttgtcggtt ccagcgtcgc catcgccgaa gcggacccgg cggtattact ggcctatctg
     8521 ttcgccggat tactggtggt tatgattatg cggatgctgg cggaaatggc agttgccacg
     8581 cccgataccg gttcgttttc cacctatgcc gataaagcca ttggccgctg ggcgggctat
     8641 accatcggct ggttgtactg gtggttttgg gtactggtta tcccactgga agccaacatc
     8701 gccgccatga tcctgcactc gtgggttcca ggcattccca tctggttatt ttccctcgtc
     8761 attaccctcg ccttaactgg cagtaattta ttaagcgtta aaaactacgg cgaatttgag
     8821 ttctggctgg cgctgtgcaa agtcatcgct atcctggcct ttattttcct tggtgcagtc
     8881 gcaattagcg gtttttaccc ttatgccgaa gtgagcggga tctcaagatt gtgggatagc
     8941 ggcggcttta tgcccaacgg tttcggtgcg gtattaagcg cgatgttgat caccatgttc
     9001 tcgtttatgg gcgcagaaat tgtcaccatt gccgccgcgg aatccgacat gccggaaaaa
     9061 catattgtcc gcgccaccaa ctcggttatc tggcgtattt ctatcttcta tttgtgctct
     9121 atttttgtcg tcgtggcatt aataccgtgg aatatgccgg ggctgaaagc cgttggatct
     9181 tatcgctcgg ttctggaatt gctcaatatt ccccatgcga aattaatcat ggactgtgtg
     9241 atattacttt ccgtaaccag ttgcctgaac tcggccctgt ataccgcctc aaggatgctc
     9301 tactccttaa gccgtcgcgg tgatgctccc gcggtaatgg gtaaaatcaa ccgcagtaaa
     9361 accccgtacg tggcggtgtt actctccacc ggcgcagcat tcttaacggt ggtggtgaac
     9421 tattacgcgc ctgcgaaggt atttaaattt ctgatcgaca gctccggcgc tatcgccctg
     9481 ctggtttatt tagtcatcgc cgtttcacag ttgcggatgc gcaaaattct gcgagcagaa
     9541 ggaagcgaaa ttcgcttgcg aatgtggctt gatccctggc tcacctggct ggtcatcggc
     9601 tttattacct ttgtgttggt agtgatgcta ttccgtccgg cgcaacagtt agaagtgatc
     9661 tccaccggct tattagcgat agggattatc tgtaccgtgc caattatggc tcgctggaaa
     9721 aagctggtat tgtggcaaaa aacacccgtt cataatacgc gctgataatg atcaggagtc
     9781 acaccatgac cattacgtct ctggatggct atcgctggct gaagaacgat attattcgcg
     9841 gtaattttca accggatgaa aaattacgga tgagtttact gacatcgcgt tatgcacttg
     9901 gcgttgggcc gttgcaggaa gcactttctc aactggtggc ggaacggctg gtcacggtgg
     9961 tgaatcaaaa agggtatcgg gtggcgtcta tgtcagagca ggagctgctc gatattttcg
    10021 acgcccgcgc caatatggaa gcgatgctgg tcagtctggc gattgcccgc ggtggcgatg
    10081 aatgggaggc agacgttctc gcaaaagcgc atctgctcag taagctggaa gcctgtgacg
    10141 ccagcgagaa aatgcttgat gagtgggatc tgcgtcatca ggcgtttcat acagcaattg
    10201 tggcgggctg tggttctcac tatttgctgc aaatgcgtga acggttgttt gatctggcgg
    10261 cgcgttatcg atttatctgg ctgcggcgaa cggtgctttc ggtggaaatg ctggaggata
    10321 aacacgatca gcaccagacc ctgactgcgg cggtactgcc gcgagataca gcgcgcgcca
    10381 gtgagttaat gcgccagcat ttactgacgc caattcccat tatccagcag gcgatggctg
    10441 gcaattaact actcttccgg aatacgcaac acttgccccg gataaatttt atccgggctt
    10501 tttagcatcg gtttattcgc ttcgaagatt ttattgtaca gattagcgtt accgtagacc
    10561 tgtttggaaa tggcactcag agtgtcgcca gacttaacgg tataaaactg gctggcagtg
    10621 gctggtgtcg ccgttttcac ctgatcatca acactggcaa taccggaaat attccccacc
    10681 gcaacaagga ttttctcctt cgcctcctga ctcaggccgt caccagtgac cgtcgctttg
    10741 ccgtcggcaa tttgaatgtt cactttatcg gcatcaggta taccggtttt gctcagatgc
    10801 tcctgcacct tcttcgcctg atcgtcttta tcgtgctgac ctgtaaccgc atcccagagt
    10861 ttttctccgg catctttaac aaaattgaac agacccatag ctacctcatt cgttaacgga
    10921 aacactcaga aagtgtagca gaggaacgcg gcgcgttaac gccgcggggg tgagcacagg
    10981 tttaatcgcg ggtttgcacc cagaacgcgt gaatcaaacc ggggatatac ccaagcagtg
    11041 tcagcagaat attaataatg aacgcccaac cgaacccttt accgagcagc acgccgatcg
    11101 gcggcagaat gatggtgatg acgattctcc agaaacccat atgtactccc tataagaaaa
    11161 ttactcattg tttaaaaaga gattttatct cttaagcgta gtaattttag cgga
//
LOCUS       DAJTYM010000114        11111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTYM010000114 DAJTYM010000000
VERSION     DAJTYM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO575_003315"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO575_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6666164.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO575_003316"
     tRNA            complement(621..695)
                     /locus_tag="OO575_003316"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO575_003317"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO575_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO575_003318"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO575_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6666165.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO575_003319"
     CDS             complement(2932..3990)
                     /locus_tag="OO575_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666166.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO575_003320"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO575_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6666167.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO575_003321"
     CDS             6068..6352
                     /locus_tag="OO575_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6666168.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO575_003322"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO575_003322"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6666169.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO575_003323"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO575_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6666170.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO575_003324"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO575_003324"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6666171.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2778 a         2543 c         2747 g         3043 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt c
//
LOCUS       DAJTYM010000115        11084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTYM010000115 DAJTYM010000000
VERSION     DAJTYM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..375
                     /locus_tag="OO575_003325"
     CDS             <1..375
                     /locus_tag="OO575_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666172.1"
                     /translation="LYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKE
                     QFSGAVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQD
                     QCLRALHHLRVQSTSKTAPQAK"
     gene            complement(578..931)
                     /locus_tag="OO575_003326"
     CDS             complement(578..931)
                     /locus_tag="OO575_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6666173.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1069..2715)
                     /gene="groL"
                     /locus_tag="OO575_003327"
     CDS             complement(1069..2715)
                     /gene="groL"
                     /locus_tag="OO575_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6666174.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2759..3052)
                     /locus_tag="OO575_003328"
     CDS             complement(2759..3052)
                     /locus_tag="OO575_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6666175.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3328..4584
                     /gene="yjeH"
                     /locus_tag="OO575_003329"
     CDS             3328..4584
                     /gene="yjeH"
                     /locus_tag="OO575_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6666176.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(4600..5076)
                     /gene="fxsA"
                     /locus_tag="OO575_003330"
     CDS             complement(4600..5076)
                     /gene="fxsA"
                     /locus_tag="OO575_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6666177.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5413..6849
                     /gene="aspA"
                     /locus_tag="OO575_003331"
     CDS             5413..6849
                     /gene="aspA"
                     /locus_tag="OO575_003331"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6666178.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6967..8268
                     /gene="dcuA"
                     /locus_tag="OO575_003332"
     CDS             6967..8268
                     /gene="dcuA"
                     /locus_tag="OO575_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6666179.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8384..8722
                     /gene="cutA"
                     /locus_tag="OO575_003333"
     CDS             8384..8722
                     /gene="cutA"
                     /locus_tag="OO575_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6666180.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8698..10395
                     /gene="dsbD"
                     /locus_tag="OO575_003334"
     CDS             8698..10395
                     /gene="dsbD"
                     /locus_tag="OO575_003334"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6666181.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10432..11007
                     /locus_tag="OO575_003335"
     CDS             10432..11007
                     /locus_tag="OO575_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6666182.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2617 a         2908 c         2747 g         2812 t
ORIGIN      
        1 ttatacgacg ttgactgcca ggacaatggc aatctggaat atgacaccta ttcccaaccg
       61 gaatggaaac ataacttgtt cgaccattat ctggcggtgc tttaccgctt taaagatgaa
      121 agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac gccgggtaaa
      181 gaaatagagg cgatcactcg ccgaatgctt gatttcagcc cacgactgaa aaaactcgcg
      241 ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga ataacgccca accgctaacc
      301 caggatcaat gcttacgcgc gctgcatcac ttacgtgtac aatccaccag caaaactgca
      361 ccacaggcga aataatctca tctttgctaa tgcgaggctg gagccatcca gcctgaaatg
      421 actgataacg tgatcgttgg tcatgccgga tgcggcgtaa acgccttatc cggcctacaa
      481 attctcaagc attcaatcag ttgcaatgcc tgctgtaggc ctgataagcg aagcgcatca
      541 ggcaataata cgtttgtcac ccagcctctg gatcacatca gttcgggcac ttataaacct
      601 gaccgataat ctggctatcc gtcgggacaa aactggacaa cattccctgc gacgggctgc
      661 tgatgccata aatcacgtta ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg
      721 cgccgcgcat agaaccgccc tcttctccgt gttgcccgga aagccagttg ctttgcttac
      781 ctgtcgcagt accaatcagc tggcactctg cgcctggctg ctcgtccaca atgcgtacac
      841 tctgaccggc agcactcaat tcgttgctgg agctacaacc cgccatcagt agcgcggcac
      901 cgacaatccc tgctaagtat tttacgtgca tgttattccc cataatcaat gagctggacg
      961 ccactcgcgt catccgtgtc tgaatcttat actagaaaga tgaccaaaag aaaaaccccc
     1021 agacatttct gcccgggggt ttgtttattt ctgcgaagtg cagggcaatt acatcatgcc
     1081 gcccatgcca cccatgccgc ccataccgcc agcagcgcct aagtcagctg catcgttttt
     1141 cggcaggtcg gtaaccatgc attcggtggt gatcatcagg ccagccacag aagctgcgta
     1201 ctgcagagca gaacgggtta ctttggttgg gtccaggata cccatgtcga tcatgttgcc
     1261 gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc
     1321 aacaacagac ggttcttcgc cgcagttcag gacgatctga cgcagcggag cttccattgc
     1381 acgcagtgca actttgatac ccacgttctg gtcttcgttc tgaccacgca ggtcagccag
     1441 tttagacgct acgcggatca gcgcaacacc accaccagca accacgcctt cttctaccgc
     1501 agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt ttctctttca tttcaacttc
     1561 ggtagcagca cctactttga taactgcaac gccgcctgcc agtttcgcta cgcgctcctg
     1621 cagtttttca cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac
     1681 acggccctgg attgcagctt cttcacccac gccatcgatg atagtggtgg tgtctttgtt
     1741 gatcacaaca cgtttagcct gacccaggtc ttccagggtt gctttttcca gctccatacc
     1801 gatctcttca gagattacgg taccgccagt cagggttgcg atatcctgca gcatagcttt
     1861 acgacgatcg ccgaagcccg gagctttaac cgcagcaact ttcacgatgc cacgcatggt
     1921 gttaacaacc agagttgcta gcgcttcgcc ttcaacatct tcagcgatga tcagcagcgg
     1981 tttgcctgct ttggcaacag cttccagaac cggcagcatt tcgcggatgt tggagatttt
     2041 cttgtcagcc agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt
     2101 gatgaagtaa ggagacaggt agccacggtc gaactgcata ccttcaacca cgtccagttc
     2161 gtcctgcaga ccggtaccgt cttcaacggt gataacgcct tctttaccga ctttgtccat
     2221 cgcttcagcg atcagtttac ctacggtttc gtcggagtta gcggagatgg taccaacctg
     2281 agcaatcgct ttagaatcag agcacggtac ggacagcgct ttcagttctt caactgcagc
     2341 ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaacagc
     2401 tttcagacct tcagtgatga tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc
     2461 tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc tgcgcaccca tattttcgaa
     2521 cttgtcttcc agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc
     2581 accgaaagat ttatccagaa ctacgttacg gccttttgga ccgagggtaa ctttcactgc
     2641 atctgccagt acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac
     2701 gtctttagct gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgcggatt
     2761 acgcttcaac aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga
     2821 tcttctcgga tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacgt
     2881 ccagcggctt cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc
     2941 cgcgggtgga tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt
     3001 caacttcttt acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact
     3061 ctcctttgag aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc
     3121 tggtgaccag agaaatgggg atgaggcttc gccccttcaa gggggaaaaa gaaaaaaaat
     3181 tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgcaatttct
     3241 tcatcgccgt cataagcgaa tctgattgtg ctaccatcga aaatctacgc agttgcccaa
     3301 aatttgggcg caatcggata tcaacccatg agtggactca aacaagaact ggggctggcc
     3361 cagggcattg gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc cgttcctgcg
     3421 ttagctgcac tggtagcagg caataacagc ctgtgggcgt ggcccgtttt gattatctta
     3481 gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag cgcaggcggc
     3541 gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac cggctggctg
     3601 tttttatcgg tcattcccgt gggtttgcct gccgcactac aaattgccgc cggattcggc
     3661 caagcaatgt ttggctggca tagctggcaa ctgttgttgg cagaactcgg tacgctggcg
     3721 ttggtgtggt atatcggtac tctcggtgcc agttccagtg ctaatctaca aaccgttatt
     3781 gccggactta tcgtcgcact gattgtcgct atctggtggg cgggcgatat caaacctgcg
     3841 agtatcccct tccccgcgcc aggaaatatc gaacttaccg ggttatttgc tgcgttatca
     3901 gtgatgttct ggtgttttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa
     3961 aatccagagc gtgattttcc tcgtgctttg atgattggtc tgctgctggc aggattagtc
     4021 tactggggct gtacggtagt cgtcttacac ttcgacgcct atggtgaaca aatggcggcg
     4081 gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg taggagcgtt atggattgcc
     4141 tgcgtaattg gctatctggc ctgctttgcc agtcttaaca tttatataca gagcttcgcc
     4201 cgcctggtct ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct
     4261 cgccatatcc cgaataatgc cctcaatgcg gtgcttggct gctgcgtggt gagcacgttg
     4321 gtaattcatg ccttagagat caatctggac gctcttatta tttatgccaa tggcatcttt
     4381 attatgattt atctgttatg catgctggca ggctgtaaat tattgcaagg tcgttatcga
     4441 ctactggcgg tggttggcgg gctgttatgc gttctgttac tggcaatggt cggctggaaa
     4501 agtctctatg cgctgatcat gctggtgggg ttatggctgt ttctgccaaa acgaaaaacg
     4561 ccggaaaatg gcataaccac ataatccggc gtttcgacat taatcctggc gatcgtcttt
     4621 atgatcaagg cggtcgcggt catcatcctt tcgctgatac tcaccatcaa aagtattacc
     4681 gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga gaaaagcgca aatgtggcat
     4741 caacttcact gtcagatgct tttgcaccgg cggcaataaa agcagaagac cgaggaagtc
     4801 ggtgaaaaag cccggtaata aaagcagcaa accggcaata atcagcgaaa cacttttaat
     4861 catctccgcc gcagggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt
     4921 aaagccctgg ttacgtacca gtgacatacc gataactgac gtgaatataa ccagcacgag
     4981 ggtcagcaat acccccaata catgggcaac ctgaataaag attgaaatct caatatagac
     5041 ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt ttctcctgta atagcagccg
     5101 gttaaacccg gctacctgaa tgggttgcga atcgccttca gcttatattg tggtcattag
     5161 caaaatttca agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatctatt
     5221 tcacaaatta ataattaagg ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga
     5281 aatcctcaag cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg
     5341 aaaaacattc gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa
     5401 aagaaggttc acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa
     5461 gttccagctg atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc
     5521 agcaacaaca aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa
     5581 gccgcagcta tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc
     5641 attgccgcat gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac
     5701 gtctaccagg gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat
     5761 atcggtctgg aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac
     5821 catgttaaca aatgtcagtc cactaacgac gcctacccga ccggtttccg tatcgcagtt
     5881 tactcttccc tgattaagct ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt
     5941 aaagctgtcg aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta
     6001 ccgatgaccc tcggtcagga attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa
     6061 aacatccaac gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt
     6121 actggtctga acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc
     6181 actggcttcc catgcgtacc ggctgaagac ctgatcgaag cgacctctga ctgcggcgct
     6241 tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac
     6301 gacctgcgct tgctctcttc tggtccacgt gccggcctga acgagatcaa cctgccggaa
     6361 ctgcaggcgg gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt
     6421 aaccaggtat gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca
     6481 ggtcagctgc agttgaacgt tatggaaccg gtcattggcc aggccatgtt cgaatccgtt
     6541 cacattctga ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct
     6601 aacaaagaag tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac
     6661 ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag
     6721 agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt
     6781 ttctccgtac agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc
     6841 gaacagtaat cgtacagggt agtacaaata aaaaaggcac gtcagatgac gtgccttttt
     6901 tcttgtgagc agtaacttaa aaataacaat ctaatatcaa cttgttaaaa aacaaggaag
     6961 gctaatatgc tagttgtaga gctcatcata gttttgctgg cgatcttctt gggcgccaga
     7021 ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt
     7081 ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc ggttatcgcc
     7141 gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag
     7201 ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac ctatttcctg
     7261 actatctttg ctggtactgg caacatctct ctggcgacac tgccagttat cgctgaagtt
     7321 gcgaaggaac aaggcgttaa accttgccgt ccgctctcta ctgcagtggt atccgcgcag
     7381 attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtgatggaa
     7441 ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg
     7501 gttctggtga tgtccttcct ggtcactatg ctgttcaact ccaaactctc tgacgatccg
     7561 atttatcgca agcgtctgga agagggcttg gttgaactgc gcggtgaaaa gcagattgaa
     7621 atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt
     7681 atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccgct gatgaacacc
     7741 accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa
     7801 gtcgataccg acaacatcct caactccagc accttcaaag caggtatgag cgcctgtatt
     7861 tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat cgactggatc
     7921 aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc
     7981 tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggct
     8041 ctggcactga acgtttcacc gctgaccgct gttgcttctt tcgctgcggt gtctggtctg
     8101 ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtact
     8161 acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt
     8221 gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg atcaatcgcg
     8281 gggcgttcac gccccgcttt ctttcccgcc gactaacatc cttcccccgt ccgttgtata
     8341 gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag
     8401 ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg gatgaagcga cagcccagga
     8461 tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga tccccggcgc
     8521 tacctctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc agatgatttt
     8581 aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc atcatccata
     8641 tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt acctctcatg
     8701 gctcaacgca tctttacgct gattctgcta ctttgcagca cttccgtttt tgccggatta
     8761 ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttgc ttttgatttt
     8821 cagcaaaacc aacatgacct taatctgacc tggcagatca aagacggtta ctacctctac
     8881 cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccgacgagca gctgccgcaa
     8941 ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga tcggctgacg
     9001 cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc
     9061 tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg
     9121 gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc agcaagagca gcacaccgcg
     9181 caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca
     9241 tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc tgggtggtaa acagcggctc
     9301 tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc actgacttac
     9361 acggcgctgg gtctggtagt tgccgccgca gggttacagt tccaggcggc gctacagcac
     9421 ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc
     9481 ttgtttactc tacaactccc ctcttcgctg caaacacgtc tcacgttgat gagcaatcgc
     9541 caacagggcg gctcacctag cggtgtgttt gttatggggg cgattgccgg actgatctgt
     9601 tcaccatgca ccaccgcacc gcttagcgcg attctgctgt atatcgccca aagcgggaac
     9661 atgtggccgg gcggcggcac gctttatctc tatgcgttgg gcatgggcct gccgctgatg
     9721 ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc
     9781 aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga gcgagtgatt
     9841 ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc
     9901 tttatcacca gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat aatcctgctg
     9961 gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc gacgcatacc
    10021 gcgcaaactc aaacgcatct caactttaca caaatcaaaa cggtagatga gttaaatcag
    10081 gcgctcgttg aagccaaagg caaaccggtg atgttagatc tttatgccga ctggtgcgtc
    10141 gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa agcgttagca
    10201 gacacggtct tacttcaggc caacgtcacg gccaacgacg cacaagatgt ggcgctgtta
    10261 aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca aggccaggag
    10321 catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc acatttgcgc
    10381 gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt
    10441 gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc caacaccacg
    10501 ctggagatgg ttgctgaacg tgtggattat ccactggacg agctacgccg cttctggcca
    10561 gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat agatgtctgg
    10621 cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagctgct ggcacgttat
    10681 caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctatt tatcgctgcc
    10741 tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca gcaaaaaagc
    10801 gctgcctacg atttcaccca cgaactgtta accacgctgg aagttgacga tccggcgatg
    10861 gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt
    10921 agccaggcgg atgtcgacac tgcacatcgg ctggcggaag atatcctgcg cttcgcccgc
    10981 tgccgccagg gtggtgcatt gacctgacag aaacacagaa aagaagcgat ttgccgcaat
    11041 cttaagcagt tgaatcgctt ttactgaaat taggttgacg agat
//
LOCUS       DAJTYM010000116        10941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTYM010000116 DAJTYM010000000
VERSION     DAJTYM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..78)
                     /gene="yjfN"
                     /locus_tag="OO575_003336"
     CDS             complement(<1..78)
                     /gene="yjfN"
                     /locus_tag="OO575_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6666183.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEF"
     gene            complement(227..556)
                     /gene="bsmA"
                     /locus_tag="OO575_003337"
     CDS             complement(227..556)
                     /gene="bsmA"
                     /locus_tag="OO575_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6666184.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            738..1487
                     /gene="yjfP"
                     /locus_tag="OO575_003338"
     CDS             738..1487
                     /gene="yjfP"
                     /locus_tag="OO575_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6666185.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(1484..2239)
                     /gene="ulaR"
                     /locus_tag="OO575_003339"
     CDS             complement(1484..2239)
                     /gene="ulaR"
                     /locus_tag="OO575_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6666186.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2347..3411)
                     /gene="ulaG"
                     /locus_tag="OO575_003340"
     CDS             complement(2347..3411)
                     /gene="ulaG"
                     /locus_tag="OO575_003340"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6666187.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            3766..5163
                     /gene="ulaA"
                     /locus_tag="OO575_003341"
     CDS             3766..5163
                     /gene="ulaA"
                     /locus_tag="OO575_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6666188.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5179..5484
                     /gene="ulaB"
                     /locus_tag="OO575_003342"
     CDS             5179..5484
                     /gene="ulaB"
                     /locus_tag="OO575_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6666189.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            5494..5958
                     /gene="ulaC"
                     /locus_tag="OO575_003343"
     CDS             5494..5958
                     /gene="ulaC"
                     /locus_tag="OO575_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6666190.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            5972..6622
                     /gene="ulaD"
                     /locus_tag="OO575_003344"
     CDS             5972..6622
                     /gene="ulaD"
                     /locus_tag="OO575_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6666191.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            6632..7486
                     /gene="ulaE"
                     /locus_tag="OO575_003345"
     CDS             6632..7486
                     /gene="ulaE"
                     /locus_tag="OO575_003345"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6666192.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            7486..8172
                     /gene="ulaF"
                     /locus_tag="OO575_003346"
     CDS             7486..8172
                     /gene="ulaF"
                     /locus_tag="OO575_003346"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6666193.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8301..8576)
                     /locus_tag="OO575_003347"
     CDS             complement(8301..8576)
                     /locus_tag="OO575_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6666194.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            8903..9298
                     /gene="rpsF"
                     /locus_tag="OO575_003348"
     CDS             8903..9298
                     /gene="rpsF"
                     /locus_tag="OO575_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6666195.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9305..9619
                     /gene="priB"
                     /locus_tag="OO575_003349"
     CDS             9305..9619
                     /gene="priB"
                     /locus_tag="OO575_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6666196.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            9624..9851
                     /gene="rpsR"
                     /locus_tag="OO575_003350"
     CDS             9624..9851
                     /gene="rpsR"
                     /locus_tag="OO575_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6666197.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            9893..10342
                     /gene="rplI"
                     /locus_tag="OO575_003351"
     CDS             9893..10342
                     /gene="rplI"
                     /locus_tag="OO575_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6666198.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(10413..>10941)
                     /locus_tag="OO575_003352"
     CDS             complement(10413..>10941)
                     /locus_tag="OO575_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6666199.1"
                     /translation="HEATLDNIHAQKYNKAYAGARLTAELKYLLQDVESFEPNSKYTI
                     HEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCCSSELPTIDLYIDQLHTVTGVSNALG
                     FIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSDRMHLTPANHP
                     DLWTQENGEWIYLRN"
BASE COUNT         2594 a         2700 c         2884 g         2763 t
ORIGIN      
        1 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
       61 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      121 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      181 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      241 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      301 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
      361 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
      421 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
      481 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
      541 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
      601 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
      661 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
      721 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
      781 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
      841 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
      901 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
      961 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1021 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1081 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1141 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1201 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1261 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1321 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     1381 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     1441 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     1501 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     1561 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     1621 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     1681 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     1741 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     1801 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     1861 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     1921 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     1981 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2041 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2101 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2161 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2221 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2281 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     2341 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     2401 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     2461 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     2521 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     2581 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     2641 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     2701 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     2761 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     2821 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     2881 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     2941 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3001 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3061 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3121 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3181 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3241 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3301 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     3361 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     3421 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     3481 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     3541 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     3601 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     3661 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     3721 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     3781 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     3841 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     3901 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     3961 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4021 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4081 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4141 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4201 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4261 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4321 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     4381 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     4441 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     4501 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     4561 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     4621 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     4681 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     4741 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     4801 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     4861 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     4921 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     4981 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5041 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5101 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5161 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5221 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5281 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     5341 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     5401 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     5461 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     5521 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     5581 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     5641 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     5701 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     5761 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     5821 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     5881 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     5941 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6001 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6061 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6121 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6181 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6241 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6301 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     6361 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     6421 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     6481 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     6541 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     6601 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     6661 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     6721 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     6781 tgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     6841 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     6901 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     6961 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7021 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7081 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7141 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7201 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7261 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7321 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     7381 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     7441 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     7501 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     7561 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     7621 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     7681 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     7741 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     7801 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     7861 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     7921 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     7981 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8041 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8101 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8161 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8221 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8281 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     8341 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     8401 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     8461 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     8521 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     8581 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     8641 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     8701 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     8761 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     8821 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     8881 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     8941 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9001 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9061 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9121 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9181 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9241 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9301 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
     9361 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
     9421 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
     9481 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
     9541 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
     9601 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
     9661 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
     9721 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
     9781 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
     9841 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
     9901 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
     9961 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10021 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10081 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10141 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10201 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10261 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10321 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    10381 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    10441 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    10501 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    10561 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    10621 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    10681 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    10741 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    10801 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    10861 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    10921 ttatctaatg tagcttcatg g
//
LOCUS       DAJTYM010000117        10864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTYM010000117 DAJTYM010000000
VERSION     DAJTYM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            597..794
                     /locus_tag="OO575_003353"
     CDS             597..794
                     /locus_tag="OO575_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666200.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO575_003354"
     CDS             complement(744..1937)
                     /locus_tag="OO575_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6666201.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO575_003355"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO575_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6666202.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO575_003356"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO575_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6666203.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO575_003357"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO575_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6666204.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO575_003358"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO575_003358"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6666205.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO575_003359"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO575_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6666206.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO575_003360"
     CDS             complement(10573..10716)
                     /locus_tag="OO575_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666207.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
BASE COUNT         2700 a         2746 c         2838 g         2580 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actg
//
LOCUS       DAJTYM010000118        10759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTYM010000118 DAJTYM010000000
VERSION     DAJTYM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            132..962
                     /locus_tag="OO575_003361"
     CDS             132..962
                     /locus_tag="OO575_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6666208.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1103..1876)
                     /gene="uxuR"
                     /locus_tag="OO575_003362"
     CDS             complement(1103..1876)
                     /gene="uxuR"
                     /locus_tag="OO575_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6666209.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2091..3551)
                     /locus_tag="OO575_003363"
     CDS             complement(2091..3551)
                     /locus_tag="OO575_003363"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6666210.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3632..4816)
                     /gene="uxuA"
                     /locus_tag="OO575_003364"
     CDS             complement(3632..4816)
                     /gene="uxuA"
                     /locus_tag="OO575_003364"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6666211.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5156..6499
                     /gene="gntP"
                     /locus_tag="OO575_003365"
     CDS             5156..6499
                     /gene="gntP"
                     /locus_tag="OO575_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6666212.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6750..7652)
                     /gene="fimH"
                     /locus_tag="OO575_003366"
     CDS             complement(6750..7652)
                     /gene="fimH"
                     /locus_tag="OO575_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6666213.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7672..8175)
                     /gene="fimG"
                     /locus_tag="OO575_003367"
     CDS             complement(7672..8175)
                     /gene="fimG"
                     /locus_tag="OO575_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6666214.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8188..8718)
                     /gene="fimF"
                     /locus_tag="OO575_003368"
     CDS             complement(8188..8718)
                     /gene="fimF"
                     /locus_tag="OO575_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6666215.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8728..>10759)
                     /gene="fimD"
                     /locus_tag="OO575_003369"
     CDS             complement(8728..>10759)
                     /gene="fimD"
                     /locus_tag="OO575_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6666216.1"
                     /translation="NSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHI
                     ITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGI
                     ARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPY
                     SSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRY
                     RAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVG
                     YRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQ
                     LGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQML
                     ALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQ
                     TGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTL
                     GQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADN
                     VDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIV
                     ADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2579 a         2779 c         2651 g         2750 t
ORIGIN      
        1 gacagtcact gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact
       61 tctcttttta ctacagaaaa atttatctat ttttctaaac atcagttaaa tactcataaa
      121 aggtaactat tatgtctatg ccattaagca atgccgctaa taaccaattc aatacaaata
      181 atcacttcct tcatcatcca aaagtcgaca gtgagttaac acgtcgatat gaatatgcac
      241 gcctggatac ggaaaatatc tatcttctgc ctcttgctcg tggcaataac cataattacg
      301 atggaaaaag cgttgtcgaa attagaaaac ttaatattgg caaacagtcc tggccattta
      361 attatgtgac agatacatgc agagaattca atggtattac aacaacagga cgtatgctct
      421 atcgaaactt gaaaatcacc tcagctttag acgaaatata tggtggtata tgtaaaaagg
      481 cgcatgctgc tacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt
      541 tcgatcctgt tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc
      601 ctggttatgc cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa
      661 gatggaccaa tgagcagcca agaattgata tctatattga tcagataata actgtctcag
      721 gggtagcaaa ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagatgg
      781 gaaatgaccc aatcattggt atagaagcct atcctggtac cgcggagatc cattcaaaaa
      841 tgggatataa cgttatcccc ggtgatgaag atgcgccact caaacgcatg actctgcaac
      901 cgtcatctct tccagaactg tttgaattaa aaaatggtga atggaattat attggtaaat
      961 agccactgag aagaatgagt actaatattc aggttataca gatatttaaa caaaaccctg
     1021 caatttacaa agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacgttta
     1081 cccttgcgct tattataata agtcaggcgt cgactttatc cagcggccat gaatcaaaga
     1141 gatagccgtc aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt
     1201 tttccagatg ctgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg
     1261 cgaggatctg tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc
     1321 tgtgcaactg gatccacatt ggattgtttt cccgccactg ccaggactga cggaataact
     1381 ccaccagcat gctgttatgc gttgcttcag caatggcgag atggaactgc atgtcaccgc
     1441 tttcgctgct acccggcgcg ctggaagcca gttcacgctc ttccagttgc aatgcctgac
     1501 gcattttgac gatatcttcg cgggtagcct gcagtgcggc aaactcggcg atgttacttt
     1561 ccagtaactg acgcgcctgt aacagctcaa aaggacccgc atcattgcag acgttggcat
     1621 ccggactgtc tgtgttgtgg ctgcctgagc tatcaagcac atagataccg gcaccccggc
     1681 gcacttccac cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtccgcg
     1741 tgacatcaag catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtacg
     1801 gcgtctttat gatcagatcg tggatcatcg ccccgacttc ctggtaaggt ctttgcgcag
     1861 aggtgacaga tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat
     1921 gcggcgtgaa cgccttatcc ggcctacaac gggcaatttt acggcccccg ttaatctcaa
     1981 actataccat tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc
     2041 aaccaaatat cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca
     2101 gccacacact cgcgcgcacc gcgttcgcac agctgctgat atgccgacgt caccgcgcca
     2161 acaaagtcgg cattctgcgg cagatcatcg gcaaaaatac cgctcaggcc cagcagcgct
     2221 ttcacgcggt ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg
     2281 tcgaccacgt caatggcatt accctgctca tccacgccct gggtgtaacc catccagcca
     2341 gccacgccca gcgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccggg
     2401 tccagtagac gctgcggtaa cttctggctg ccgtccatcg caatctgcca ggtacggtga
     2461 cgcagagacg ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtccgta
     2521 ccttccggca tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg
     2581 cgataatccg ggttagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg
     2641 tacgccagga aagagtggct gccgttcagc atacgcagct tcatcatttc gaacggcaca
     2701 acgtctgcaa cgaactgtgt gcccacttta tcccagtccg ggcgaccatt aacgaagtta
     2761 tcttcaatca cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc
     2821 agctggtcag caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc
     2881 atggtgcacg ggaaggtgac gttctcttca atccatgccg ctagctgcgg gtcacgcgcc
     2941 tgagccagcc ccagtaccgc gaccttcgcc acatggccgt tttcacgcac gttatcgcag
     3001 gacatcaccg taaatgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg
     3061 taaccaatcg cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta
     3121 ttgagatcca gttgaccgct tgccgcatca gcgcagtagc ctttttccgt gaccgttaga
     3181 gagacaatcg ccgtttgcgg acgcgccatc gcgttgagaa taccttcgca gccgtcgatt
     3241 tccggatgca gcgcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct
     3301 ttttccgcta cggtgtacag cagttgctgt tttttcaggt tttcgatcag catgcggtcg
     3361 ttgcctggca tcaggttaac ttcgcagatg ccccagtcgc tgtcggtgct ttccagcaga
     3421 tgatgggtat acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg
     3481 cgtgattcca gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca
     3541 atagtagtca tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa
     3601 ccggcacggc gcgcagccat gccggtgggt attaacggat aaagaaagcg cgctggatcg
     3661 ccagttcgac accgcgaact tcggccaggc ctttcagacg accaattgcg gagtaaccag
     3721 ggttggtttt cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga
     3781 tcaggtcttc tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca
     3841 ctttgtacat atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat
     3901 cttcacgcat ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca
     3961 tatcaaccag atcgttgtca gcacgcacgc cgtaggaacc ggtgcacatg gtgaaaccgt
     4021 tcgccatgct gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc
     4081 gcggcaagcc gaggatcggg cgcggcggat cgtccgggtg aaccgccata cgcacgccaa
     4141 cttcttcagc aactggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg
     4201 ctttgtcgat atctttgtac agctccaggt gcttacggaa ctggtcgagg gtataccctt
     4261 cttccgcgcc cggcagaccg gcaatgatgt tacgcgtcag acgcgctttg tcttcgtcgc
     4321 tcatagtggc gaagcgttca gctgcctgag caatttcttc ttcggtgtaa tccgcttccg
     4381 cgcctggacg tttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca
     4441 gagctttgga gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg
     4501 gcatgaagtt gtagcacacg gtgcgaatgc cacactgcgc caggttgcgc agagtctgct
     4561 gatagttagc gatccactgc tcatagttgc cagtgtgggt tttgatatct tcgtgaattg
     4621 gtacgctttc tacgacagac cacaccaggc ctgcgtcttc aacgatcgcc ttgcgtttga
     4681 ggatctcttc tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc
     4741 cagttgcgcc cgcctgacgg acatcagcta aagaaaccgg atcgtttggg ccgtaccagc
     4801 gccaggtctg ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgc
     4861 ggaaccttgg agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca
     4921 tcggatgaga tggcgtataa gttctacctt ttggtaagac atgtcaacca gaattctgga
     4981 attggttaac cgcgtcgcaa gaatttcact ctcatggttg ttgctgcaaa ttgcagcaaa
     5041 gggtaatcga tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat
     5101 ttacataaca tgtcgcccaa aataacaacg gttcaaccaa gtaaggtaaa acgttatgca
     5161 tgtgcttaac attctctggg tggtattcgg cattggcctg atgctgatac tgaatttgaa
     5221 gttcaaaatc aattcaatgg tggctttgtt ggtggcggcg ctgtccgtcg ggatgctggc
     5281 aggcatggat ttgatgtcgc tgctgcacac catgaaagcg ggcttcggca acacgctggg
     5341 agaactggct atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg
     5401 cgcggctcac cagatagcac atacgctgct ggcgcgtctc ggtctgcgct atgtacagct
     5461 gtcggtgatt atcatcggcc ttattttcgg tctggcgatg ttctatgaag tggcctttat
     5521 catgttagcg ccgctggtta ttgttattgc cgccgaagct aaaattccgt tcctgaaact
     5581 ggcgatcccg gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg
     5641 tccggtggcg ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt
     5701 actggtgacg atcccaagtg taatctgcgc aggtctgatc ctgccgaagt tcctcggcaa
     5761 tcttgagcgc ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct
     5821 gccctctttc ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac
     5881 caccatcgcc aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat
     5941 tggttcctcg ccgattgcga tgtttattgc gatggtggtt gcattcgtac tctttggcac
     6001 cgcgcgtggt catgacatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagtat
     6061 tgcaatggtg attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac
     6121 cggcattggc gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacattat
     6181 ggcatggctg atcactgtgc taattcgtct ggcgacgggt cagggtgtcg tttcggcgat
     6241 gaccgccgcc gggattatca gtgctgcaat ccttgatcca gcaaccggtc agctggttgg
     6301 cgtgaatccg gcgctgctgg tactggcgac ggctgcgggt tccaacaccc tcacccacat
     6361 taatgatgca tctttctggt tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt
     6421 gaaaacctgg ggtctgctgg agctggtcaa ctccgtggtt gggctgatta ttgtgctgat
     6481 tattagcatg gtagcgtaaa agaacaccag agcctgccaa tggcaggctc agactgatga
     6541 ctaaagtcta aaaatgtgcc cggacagtcc cctcgcccct ccggggagag ggttagggtg
     6601 aggggaacag accagtaaag tgcgctttgt ttatgccaga tgcgacgctg acgcgtctta
     6661 tctggcctac aaagggctaa cgtgcaggtt ttgtaggtct gatagcgtag cgcctcaggt
     6721 accagcatta gcaatgcgct gtaatttttt tattgataaa caaaagtcac gccaataatc
     6781 gattgcacat tccctgcagt cacctgccct ccggtacgtg cgtaatttgc cgttaatccc
     6841 agacttaccg ccgaagttcc tactgctcct aacgataccg tgttattcgc tggaataatc
     6901 gtaccgttgc gcgtcaactg tacgccgacg ccctgcgctg gtgaaaacga cgcggtattg
     6961 gtgaaaatcg agttgcccgc atctgcggtt gtgccggaga ggtaataccc caggttttgg
     7021 cttttcgcac aataaacggt aagaggaatt ggcactgaac cagggtagtc cggcagagta
     7081 acggtgacat cacgagcaga aacatcacaa ccgccagtgg gcaccaccac atcattattg
     7141 gcgtaaatat tccacacaaa ctgaaaatca tcgctgttat agttgttggt ctgtcgcaaa
     7201 ataagcacgg caattaatga accagcttta atagccaccc cgcccgcact gctcacaggc
     7261 gtcaaataaa gcgccaccgg ccacggctta tccgttctcg aattataaac aacccgcggc
     7321 gtttcgctgg tggtcggaaa tggatagcta ctgccactat attttacggt cccggaaaaa
     7381 ttagataaca cgccgccgta agccgagcct cgttgcagtg tgacatagtc tgtaatggtt
     7441 tccggataat cgttatggca aaagatttgc gtcgaaagat ctacgaccag gttttgcccc
     7501 acattcacga caggcgcaag gtttacataa acattagcgc tgccaccgcc aatagggata
     7561 gcggtaccat tggcggtttt acaggcgaat gaccaggcat ttaccgacca gcccatcagc
     7621 agtacagcaa acagggtaat agctcgtttc attacaatta tctcttcggg ttcagctgta
     7681 ggtataggtg atgctaatca ctgcctgaat ggttccctga gtggctccgc catttaccgt
     7741 caatgctctg acctgtaacg ggaagtgcgc tgattgtgag gaatcatcca cctgaactgt
     7801 tttggttgcg ccagtattca atgtgttgcc actgtcatcc tgtagctcta actggatgtt
     7861 ttgcgcggtc ccctggtttt tataatatcc ggtgctgtcg gctgccccgc tgaagctggc
     7921 agtgaccctc gacgttccca ccggacaatt agtcaactca agcgcaacat catgccaggc
     7981 cgatgccgcc ccggcagaca taagactgaa agaataaaga tcgccgagat ctaccgtggc
     8041 attggtggtg gaaaccgtac acggtttggc gacgacctta ccgttcaccg tgatggtgac
     8101 atcggctgcc tgtatcgtcg cacttgcgag cgccaacatt gccgccaata catacccacg
     8161 tttgcaccat ttcatgagca tctccagtta ctgatattca agagtgaagg tagccgtagc
     8221 attgatatgc cccgcagtga caggcacctg tgtcgccatt agccgggcgt aaaaattcag
     8281 cgtatttggt ttacccggcg tcagggtcgt ccacgaaatc gcagacgatg gagcattaag
     8341 gggtatttga ttttgctgct cattcagaag ctgtattccc agtcccgcag ccgctgacac
     8401 cgtattttca agtgcaagca ggttggcatt gtggctatct gcaacgccgg taaacccaac
     8461 ttttacggca gaaacggcat taccacaggg tgacagcaaa atacgaaatg gaacgacagg
     8521 agtcgtcgcg ccaatgttgt taaattgctt cgccgcgttt tccatcagat caacagtaaa
     8581 attggttgat tcagcggcca cactacagcc gttatctctg acatagccgc ggatagtaat
     8641 cgtgctatcc gcagccaaaa cgcgacttac cgccagccac aaaaaagcgc acagaagata
     8701 aaaaggtttg tttctcatca cgccccctta acgacattca gctgatagct gggttaataa
     8761 ctgctgctga ctctctggtg gcagttgata attggcgaca cagtgagcat tttcctcttc
     8821 tccccatttc acctgaactt ttcccgctaa aggcattccg ctgaggtaaa cctgaccatt
     8881 atccgcaaca atgccgctac tctggctact ctctgatgtc accatcgccc caaacggcag
     8941 cggcttatta ttgtgagtta gcgtcatgag cagttttatc ccaacgcgcg ctttaaactc
     9001 tgctcgcacg atcgccccac gagtgggaac aacgttagcg accgcgttat ctaaatcgac
     9061 gttatcagcc aaggtattgg tatccagcgc cactctattt tcccgatatt cagtggcata
     9121 aggcagcacg gcataaccac gccagtcggt acgcaccccc gtctggtttt cgacttttgc
     9181 atcttttgcg ccaggcgctt taacaagcac caccgtatcg tttaacggct gccccagcgt
     9241 tacgccattg gcatgagcca ataccccacc gctgactccg taatagagct gcttaatatc
     9301 atcgctatgg ctgtaaccga tattggcatt gccgtaacca ccgcgataat tcagcgtggc
     9361 gtagcctgta cttccgctat taccatcgcc tcccccggca tagccggttt gcacgctata
     9421 actgaggttg ttgtcttcca gcaacgtacc gtatacacca gccagattgg tcatccgacc
     9481 gttgagatcg tgtgacatgc tgtagctggc actggcatgt cgccactgag atttactgtc
     9541 agaacgcagc cagtggctga aaggaatatt gacgttaagc gctaacatct gatcccgtcc
     9601 tttttgccag gcgtttttcg tcaggctata gctgagcgtc cagttgatat cttcgaacgc
     9661 agtatttaat ccagcctgga attgctcatc gacattactc gttccccaat aagtttgatg
     9721 gctaccactc aaatacagtg ttgatgtgcg cccgagttgc tgagtaacgg tgagctgtaa
     9781 tttcccgcgt ttgttataag cgaggttgta atagtcggtg aatttcggct taacctgaat
     9841 aactccatcc tgtgtttcga tgttgtagcc attcattcga ctgtatgttg tatcagcgaa
     9901 attaaaatat ccgctggtcg aataacggta acccactaac tgaatattcg tgcctgattc
     9961 attgagcgat ttgttataga gaaaacgcac cgattgtccg tcatgctgac tgtcatcggg
    10021 aagtgtggaa ttagcctgcg tcatatccac agacagagcg cccagtgcct ccatgttttt
    10081 cccgataccg aaattaaaag cacgataacg atccgccagt tgcgttccac catatattgt
    10141 ccagccagcc ggaaggccgt ggagtaatgt actctggaaa aagcggggtt tttcctgctg
    10201 cgcatttcca ctacggtatt ctcctgccgt aatggaataa cgagtatgcc cttcacgttg
    10261 caaaagcggg actgacgaat agggtacggt aaaaatctgc gtgctgccgt cagcctcttt
    10321 gatcgttacc tgcaagtcac cactattacc tgcggcatag atatcgttga tggtaaaagg
    10381 ccccggtggc accgtactat tataaatgcc atatccattt tgtttaatag tgacctgtgc
    10441 agtaccacga gcaataccgt ggatcaccgg ggcaaatcct ctttggctat cgggtaacat
    10501 attgtcatct gaggccaatt gtgcgccgcg aaagttaata ccatcgaaaa tatcgccctg
    10561 ggtataacca tcacccaacg tcagccggga acgtaaagga attatgtctc gctcaatcca
    10621 ggtaatgata tgctgccatt tatttttgct acctgatgaa ctgtcgctac tgttataact
    10681 ccaggtggta ttgtcgcgta aacgccacgc accaatattt aacccactct gtaggtttaa
    10741 atatgcataa tggctgtta
//
LOCUS       DAJTYM010000119        10607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTYM010000119 DAJTYM010000000
VERSION     DAJTYM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1439
                     /gene="amn"
                     /locus_tag="OO575_003370"
     CDS             <1..1439
                     /gene="amn"
                     /locus_tag="OO575_003370"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6666217.1"
                     /translation="GLTPAQALDKLDALYEQSVVALRIAIGKYITIGELPDENARKQG
                     LFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDY
                     GAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETADGIYHPT
                     EFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGCSQILDP
                     DSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLVNIGVGP
                     SNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAVLPPDIP
                     IPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASALRFNLSR
                     AVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAISEHLQIG
                     IRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            1782..2498
                     /locus_tag="OO575_003371"
     CDS             1782..2498
                     /locus_tag="OO575_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6666218.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(2954..3029)
                     /locus_tag="OO575_003372"
     tRNA            complement(2954..3029)
                     /locus_tag="OO575_003372"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2994..2996),aa:Asn,seq:gtt)
     gene            complement(3130..4757)
                     /gene="yeeO"
                     /locus_tag="OO575_003373"
                     /pseudo
     CDS             complement(3130..4757)
                     /gene="yeeO"
                     /locus_tag="OO575_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4762..4837
                     /locus_tag="OO575_003374"
     tRNA            4762..4837
                     /locus_tag="OO575_003374"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4795..4797,aa:Asn,seq:gtt)
     gene            complement(4875..5825)
                     /gene="cbl"
                     /locus_tag="OO575_003375"
     CDS             complement(4875..5825)
                     /gene="cbl"
                     /locus_tag="OO575_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6666219.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(5927..6844)
                     /gene="nac"
                     /locus_tag="OO575_003376"
     CDS             complement(5927..6844)
                     /gene="nac"
                     /locus_tag="OO575_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6666220.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7172..7247
                     /locus_tag="OO575_003377"
     tRNA            7172..7247
                     /locus_tag="OO575_003377"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7205..7207,aa:Asn,seq:gtt)
     gene            complement(7303..8238)
                     /gene="ldtA"
                     /locus_tag="OO575_003378"
     CDS             complement(7303..8238)
                     /gene="ldtA"
                     /locus_tag="OO575_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6666221.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8300..9379)
                     /gene="cobT"
                     /locus_tag="OO575_003379"
     CDS             complement(8300..9379)
                     /gene="cobT"
                     /locus_tag="OO575_003379"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6666222.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9391..10134)
                     /gene="cobS"
                     /locus_tag="OO575_003380"
     CDS             complement(9391..10134)
                     /gene="cobS"
                     /locus_tag="OO575_003380"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6666223.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10131..>10607)
                     /gene="cobU"
                     /locus_tag="OO575_003381"
     CDS             complement(10131..>10607)
                     /gene="cobU"
                     /locus_tag="OO575_003381"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6666224.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2965 a         2709 c         2286 g         2647 t
ORIGIN      
        1 ccggtctgac cccagctcag gcactggata aactcgacgc gctgtatgag caatctgtag
       61 ttgctttacg catcgccatt ggcaaatata ttacaattgg cgaattacct gatgaaaacg
      121 cccgtaaaca aggtcttttt gtctatccat cactgaccgt aacctgggac ggtagcacaa
      181 caaatccccc caaaacgcgc gcatttggtc gttttactca cgcaggcagc tacaccacca
      241 cgattactcg ccctactctc tttcgttcgt atctgaatga gcaactaacg ttgctttatc
      301 aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca tgaaatccct tatccttatg
      361 tcatcgatgg ctctgaattg acacttgatc gctcaatgag cgctgattta actcgctact
      421 tcccgacaac agaactggcg caaatcggcg atgaaactgc agacggcatt tatcatccaa
      481 cggaattctc cccgctatcg cattttgatg cgcgccgcgt cgatttttcc ctcgcacggt
      541 tgcgccatta taccggtacg ccagtagaac attttcaacc gttcgtcttg tttaccaact
      601 acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag ccagatcctc gatcctgata
      661 gtccctacat tgccctttct tgtgctggcg ggaactggat cactgccgaa accgaagcgc
      721 cagaagaagc catttccgac cttgcatgga aaaaacacca gatgccagca tggcatttaa
      781 ttaccgctga tggtcagggt attactctgg tgaatattgg cgtgggaccg tccaatgcta
      841 aaaccatctg cgatcatctg gcagtgctac gcccggatat ctggttgatg attggtcact
      901 gtggcggatt acgtgaaagc caggccattg gagattatgt ccttgcacac gcttatttac
      961 gcgatgacca cgttcttgat gcggttctgc cgcccgatat acctattccg agcattgctg
     1021 aagtgcaacg tgcgctttat gacgctacca agttagtcag cggcaggcct ggtgaggaag
     1081 tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga tgacaggaac tgggagttac
     1141 gttactcagc ttctgcactt cgttttaact taagccgggc cgtagcaatt gatatggaaa
     1201 gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc atacgggaca ctactgtgtg
     1261 tttcagataa accgttacat ggtgagatta agcttcccgg ccaggctaac cgtttttatg
     1321 aaggcgctat ttccgaacac ctgcaaattg gcattcgggc gatcgatttg ctgcgcgcag
     1381 aaggcgaccg actgcattca cgtaaattac gaacctttaa tgagccgccg ttccgataat
     1441 aagttatgaa ccttcttatc agaaatagat gtgaaggatt aagtaagaca tctggcaaat
     1501 agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga tgcgagtttt attatcgtct
     1561 taatgatttc cacatattaa aagcaagtat gctttcaaaa cacaattata aaaaatcccg
     1621 ccaacaacat tagtttttat aaaattaaat ataagattat ggctttagaa tatttttatt
     1681 tctaatagac gagatttttc ctgttatgat ataatatgcc gaattaacac atgttaacga
     1741 tttaccagta atgtaaataa attttcgagg agatcattcc agtgggacgt aaatgggcca
     1801 atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc taaaatttat gcaaaattcg
     1861 gtgtagaaat ctatgctgct gccaaacaag gtgaacccga tccagaatta aacacatctt
     1921 taaaattcgt tattgaacgt gcaaagcagg cacaagttcc aaagcacgtt attgataaag
     1981 caattgataa agccaaaggc ggcggagatg aaacgttcgt acagggacgt tatgaaggct
     2041 ttggtcctaa tggctcaatg attattgctg agacattgac ttcaaatgtt aaccgtacga
     2101 ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa tatcggagcg gcaggttctg
     2161 tcagctatat gtttgacaat acgggtgtga ttgtatttaa aggaacagac cctgaccata
     2221 tttttgaaat tttgcttgaa gctgaagttg atgttcgtga tgtaactgaa gaagaaggta
     2281 atattgttat ttatactgaa cctgctgacc ttcataaagg aatcgcggcg ctaaaagcag
     2341 ctggaatcac tgagttctca acaacagaat tagaaatgat tgctcaatct gaagttgagc
     2401 tttccccaga agatttagaa atctttgaag ggcttgttga tgcccttgaa gatgacgacg
     2461 atgtacaaaa agtttatcat aacgtcgcaa atctctaatt atattttaaa gaaatctgtc
     2521 tttatggcag atttctttaa tctcatataa ttcttataaa aaatataata tttaactcgt
     2581 catattgctt ataaaaaatc cccgtcccca gagaaataat atttattaaa atggcaggtc
     2641 ttctttttct gattaccgaa gacaaagtgg caattacgta tagtttcccg ataaagacgc
     2701 gacagctacg tcccgcataa ggcatttttc tctttatctt tgtacggtac ttcatggaac
     2761 agaatttttg accttgtgaa tcgtgatatc tgttggggag gggcaatttg ctcactgaag
     2821 cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa aacccggcca cttttttaca
     2881 aagtggccgg gtaaaaataa tttttatttt ttaactgtcc tgagtctcat agagatgtct
     2941 caaaactaaa atttggctcc tctgactgga ctcgaaccag tgacatacgg attaacagtc
     3001 cgccgttcta ccgactgaac tacagaggaa tcgtgagaac gaggcgaata ttagcgatgc
     3061 ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc gtttgctgaa ataatctgca
     3121 ttttgtcgtt tattccgaca caactggctt tttttcacac ttttgcggct cggatcgagg
     3181 gtatttccac agccaacgtc cagtaaccat tcgccagtaa aacagcacgg cccgcacagc
     3241 ccagtcggca aacattccca tccagacacc aaccacaccc cagccaagca tgattccctg
     3301 cacataaccg actacaaccc ggcaacccca catgctcaac atagaaaccc acatggcgta
     3361 acgggcatca cgagcaccct taaatccagc gggtagcacc catgaggcgg accaaatagg
     3421 cataaataaa gcatttagct aaatcagaat cacaacgacg tgtttaacct gtggatcctg
     3481 ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt agccaggcga tggccgttaa
     3541 tccaagagtg gacagccaga acacatgccg caactgaatc tctgcttgcg ctatctgccc
     3601 tacccccaac cttcggcctg taatgatcgt agaagcagaa ccgagcgcac ttccgggtaa
     3661 gttgataaga gccgcaattg agaacgcgat aaaatttccg gcaataacgc tggtccccat
     3721 cccggcaacg aacatttggg ttaataaccg accactggta aataacactg attcgacact
     3781 cgctggaata ccaatcccca tgacttccca gataatgcta aaattcagcg gtttaaaata
     3841 gctctttaac gaaatcctta gcgcaggatt aaaaccaatc gccagcaccc acaaaattgc
     3901 aactgcgcca atataacgag aaatggttaa acccaaccca gccccgacca ggagaaaagg
     3961 ccgtaaatca atatgccgct aataataata ttaagaatat tcaggctacc gttaatcagt
     4021 agcggtattt tcgtattccc tgcaccacga agtgccccgc taccaataag agtgatggca
     4081 gcggctggat aactgagtac ggtcagctcc agataagtca acgccagtgc tttaacttct
     4141 gtcgtggcat cacccgcgac gaaatcaata atttgttcgc caaaatgatg aattagcgtt
     4201 gccaacagta cggcaaacaa cgtcatgatc accaatgact gccgcgtcgc caccctcgct
     4261 cgtcgtcgat cccgcttacc gagactaaat gctacaacga cagtagtacc aagatcgata
     4321 gctgcaaaaa aagccataat gaccatattg aagctgtccg ccaaccccac gccggccatc
     4381 gcatcttttc ccagccaact gaccagaaaa gtgctcagga cccccatcaa caggacacag
     4441 gcgttctcca tgaagatagg aacagcaagc ggggttatct cgcgctagaa caacactttg
     4501 tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag
     4561 atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca tttcaaatga tgagggagaa
     4621 tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc aaatgcaaca aactgttgat
     4681 agaaacggca aacagttggg aaatttaaaa atcgggtttg acaaaatatt tttcgccgtt
     4741 aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg tagaacggcg gactgttaat
     4801 ccgtatgtca ctggttcgag tccagtcaga ggagccaaat ttctgaaaag cccgctttta
     4861 tagcgggctt tttgctatat ctgataatca atttcctctt cactgttttc catcacctgg
     4921 cgcttgatat cctcaactga cagtcctgca ttacaaagtt ccagaaagcg ccaaacatag
     4981 ttacgctgaa gttgtcctcg cttcagtccc aaccagacag tattagcatc gaaaagatgc
     5041 cgcgtatcca ggcggattaa attctcttcc tcttgttcgc cgctggattg ttcggcaact
     5101 aatccgatcc caagcccaag agcaacatag gttttaatga catcagaatc ctgtgcactt
     5161 aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt catcagtgcg tgagcgcccc
     5221 gtaattccct gtcggtaagt aattaacggc cacttcgcta ttgattccag cgtcaatggt
     5281 gtaatttgcg ttaagggatg atcgagtgga acaagcaaac tatggtgcca acaaaaccat
     5341 gggaaggcga cgagctgcgg gtcattactc aaacgctcgc tggcgatacc aatatcagct
     5401 tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac cctggattag ctcgagccga
     5461 acctccggga aaagttcgcg aaaagcttta atgacctctg gcaagctata acgtgcctga
     5521 gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt tggtaaacag gtctgcaagc
     5581 cgacgaacat tactggcttc attcagaata cgttctgcaa tgaccagtaa tgctttgccc
     5641 ggttcagtca tccccagcag tcgcttacct cgtcgaacaa atatttcgat gccaaattca
     5701 tcctccagtt cccgaatatg acggctaacg cctgactgtg aggtaaaaag catattcgca
     5761 acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa
     5821 ttcacggtaa actccgggca gttcagatat cccgttattg ttaaagtcta atgcccggca
     5881 taacaaataa taaaaacccg catcttattc catcccgata taacgcttag ctcaccaatt
     5941 gccactgcct tttttccatc actggagaac taatcactga cattaacaac tctttcactg
     6001 cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt taaggacaaa gagagactca
     6061 ttgaaggagt ggtaatgcgt gacatccacc catttactgc gccacataac gaacgcgcag
     6121 ccgattcagg taatactgca acgcccatgc cgctggcaat cgctgcggta agcgtggcaa
     6181 tagactcaat ttcaccaatg acttttgccg tgagccgccg tagggaaaaa gcctcatcaa
     6241 cacgaagtct aacagcgctg taatcactgg ggagaaaaag gttcatttgc gcaatagcat
     6301 taacatcaac gttttgcccc gggcaatctt gagttcctac caggaaaaga tcttctttca
     6361 gcaaagcctg actggatacc ccagccacag gggaatgctc ataaatcacc gccatatcga
     6421 gttggtgatt tatcagtttt tcgttaagca ctgcaccact attttcatga agatagataa
     6481 cgacctccgg gaattcagcg cgaaccgcct gtaataaggg catggtgatg gatgacgcag
     6541 cggttcctgg cgcaaagcca atcgagactt gccccgataa tgactgacca acgttatgca
     6601 ccgccagttg ggcctgctca cactgacgta aaatggcccg tgcatgggta tagagaattt
     6661 ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat caaaagctgt tgatttaact
     6721 caccttccag tatggcaacc tgttggctga gcgctggttg tgcgatatgc aatacttcag
     6781 cagcctgggt caggctacca atatctacaa tttttacgaa gtatttcagg cgtctgaagt
     6841 tcatgttgcc tccggttttt aagaatcggc ccaagtgccg ccattactta caaccagtat
     6901 gcaagatgcc tgccagttct attttggcgt tgatgcgcaa gctaaccaac tgtcaaataa
     6961 gagattatga tagattcgtc attggccctt tttatcagca gtcgcattca cctgccctat
     7021 aaaaggaggg tatgcaccac gatggttcat tacccaataa gattgaaagc tcaccacttt
     7081 gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca ccctttgaca tcaccatgca
     7141 ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     7201 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc
     7261 ctgctttcga gcaggctttt tactttctaa ttaccaacgc tcttaaaaca tctgtcttga
     7321 accagaacta atttgcacag gcattcccga tcgatgttgc aacgcagcat ttgcgcgatt
     7381 tacatcaact tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggcac
     7441 ttttcggtca gactcatatt ctgcacgatt gcgcgacagc ggctcatgaa cttccagcca
     7501 ctttgaacca tccggttccg ttgtgtattt cactggctga tcaataattt gtacacgagt
     7561 gcctacagga acattatcaa acagatattt gatatcgtca ttacgcagac gaatacagcc
     7621 ctgacttaca cggagcccaa taccaaaatt ggcattggta ccgtggatgg catacaacct
     7681 gccaatataa atcgcgtaca gccccatggg gttatcgggc cccgcaggaa caaatgcggg
     7741 caaactctcc cctcgtttcg catattcgcg ccggatgttc ggcgttggcg tccaggttgg
     7801 tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg
     7861 gccgatacca ataggaaaga cttccactgt attactgtct ggcggatagt aataaagacg
     7921 catttcagcg acgttaacca caatcccttt acgaacagtg gcgggcaaaa tcagttgctg
     7981 cggaatggtg agttgtgagc caggcttcgg caaaaaaaca tcagcgcccg gattcgcttc
     8041 cagcatatta cttaaccctt gcccgtattg tgcggcaaaa gtctccagcg gctgagtatt
     8101 gtgatcagga acagttacag taagcgactg ccccactaaa cggctaccct ctggaggtaa
     8161 tggataagtt accgccaggc tattttggct ggcaaaaagc agagcaaatg agcaaagaat
     8221 atttacacga cgcatcatgt ccctttccta agtcgcgaat actatccgat aagtatagct
     8281 tttatcagac ttttcgtttt tagctgttca aatcagaagt cgtattcccc ggtagaacaa
     8341 tattactggc agcaagttcg cccatgttgt tgtatatcgc acaagcagct tcgatgatgg
     8401 acatcgccag agctgcacca ctcccctcac ctaaacgcat atccatattg agataaggct
     8461 ccagccccaa atgcgagagc gctatacgcg cgcctttttc tgccgacaag tgagaaggaa
     8521 tgagatacgg tttgattgca ggagacatct ggcaggctgc gagcgcagca gcataagaaa
     8581 gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc acctaacatc actccagcta
     8641 ttccgaccaa atcaaatcca cccacttttg ccaggacatc aacaccatcc tgaggatttg
     8701 gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt attagccagt ttatctgtcg
     8761 gcaggtttgc gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta
     8821 ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtgacac
     8881 cgtttttcgc cagctcccgc gtataacata tgacgtccaa aagcaacttt tcagcctgac
     8941 ggcgactcat tgccggagct gaagcaatat tgccgctacc tcgtgcgaca cgcatgttga
     9001 taagcccggg gataggctca gcagtatcaa taccaacatc aattacgtgg acgttagcgc
     9061 ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg ggtcatattt tcagcctgta
     9121 tggctgtcac ttcttttgga gaaatagcga ccccttcctc ccagacgccg tgatcggcac
     9181 acataaccag taccgctttt ttgcccacat gcggtatgcc attcaccccc ggcattcctg
     9241 ccagttgtat ggcaagcgcc tccagctttc ctaggctacc aacaggtttg aataacccgt
     9301 caacatgccg ttgtgcacgc gacatagcgg cagaattgat ggctgggatc gtattcagta
     9361 aatcggcaat tatttgcatg tcacgtttct ttatagcagt gccagcagga agaccagttc
     9421 accaagctct atggctgctc ccagcgtatc gcccgtttgc ccgcccagcg tacgtttgag
     9481 aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat
     9541 acccagcaat aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg
     9601 cccatcaatt ttgccaatga acacattgcc aagcccttct tcccgcgcat aacgatgacg
     9661 atacatcaat aatgcggcag tcccgcggct gaccgcacat gctgccgcca gcgatgcaag
     9721 gatcggttcg ccacgcagag ccaactcgct taacaccaga atctttgcca gtaccacaaa
     9781 aatcaatgcc agaccgccgt gggtgcctaa acggctatca cgcatgattt ccaacatgcg
     9841 atcgcgacta cgtgcagaaa atacgccatc acaggtatca gcgaggccgt ccagatggaa
     9901 ttccccggtc atcagcgcca gcacaagtac gctaaacagt gccgccagtg gtgcgccaca
     9961 ccatgcctgc agcaccatga agaccagccc gctaatcgcg ccaagcaata atccaatcaa
    10021 aggaaaagta ataataccgc gagaataatg ttcgaaatcc agtccctggg accagcgacg
    10081 cggtacaggc agacgcgtaa taaatgagag catcgcccaa aataatttgc tcatttgatt
    10141 tttactccaa tacccgaaac caccagccat acttcatttg ccgcagcggc caactgctga
    10201 tttacccgcc cggcaatatc acgaaaatgt cgtgccagac gactctccgg cacaatcccc
    10261 attcccactt cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc
    10321 aacgactgaa tctcagcatt aatcgcctgt tccatcgcct gataatccca ttcatcaggg
    10381 tctttatcgc cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattccagc
    10441 aacacagcct cattcgggtt aatgtctgca tgaattaatt catcaagatg ttgccagcgc
    10501 tccactgtgc gccagtgctc cgggcggctt tgccgatgat gttctatccg tgcagccatc
    10561 tcatcatcaa ggatttgcga ggtagcgata tacagaacct gtgaaga
//
LOCUS       DAJTYM010000120        10597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTYM010000120 DAJTYM010000000
VERSION     DAJTYM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            366..449
                     /locus_tag="OO575_003382"
     CDS             366..449
                     /locus_tag="OO575_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6666225.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(503..1855)
                     /gene="gorA"
                     /locus_tag="OO575_003383"
     CDS             complement(503..1855)
                     /gene="gorA"
                     /locus_tag="OO575_003383"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6666226.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(1927..2769)
                     /gene="rlmJ"
                     /locus_tag="OO575_003384"
     CDS             complement(1927..2769)
                     /gene="rlmJ"
                     /locus_tag="OO575_003384"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6666227.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            2972..5014
                     /gene="prlC"
                     /locus_tag="OO575_003385"
     CDS             2972..5014
                     /gene="prlC"
                     /locus_tag="OO575_003385"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6666228.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5022..5774
                     /gene="rsmJ"
                     /locus_tag="OO575_003386"
     CDS             5022..5774
                     /gene="rsmJ"
                     /locus_tag="OO575_003386"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6666229.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(5823..7292)
                     /gene="dtpB"
                     /locus_tag="OO575_003387"
                     /pseudo
     CDS             complement(5823..7292)
                     /gene="dtpB"
                     /locus_tag="OO575_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7610..8044)
                     /gene="uspA"
                     /locus_tag="OO575_003388"
     CDS             complement(7610..8044)
                     /gene="uspA"
                     /locus_tag="OO575_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6666230.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8438..8773
                     /gene="uspB"
                     /locus_tag="OO575_003389"
     CDS             8438..8773
                     /gene="uspB"
                     /locus_tag="OO575_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6666231.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(8844..10343)
                     /gene="pitA"
                     /locus_tag="OO575_003390"
     CDS             complement(8844..10343)
                     /gene="pitA"
                     /locus_tag="OO575_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6666232.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
BASE COUNT         2612 a         2738 c         2825 g         2422 t
ORIGIN      
        1 ccggacgtaa aaaaaccgac ttcgcgtcgg tttttttact cttcagccca gagttggtgg
       61 ctctggctgg agtgagaatg ccatcatcta acgcatcaat gctaatacca tactgaaact
      121 attgcaaggt tatgctggtt atttagccta attacactgt atgattatcc agttagctct
      181 gaggcatttt cactctggca atgcgcataa acgctttcaa agtcctggtc agaagtacgg
      241 gtggtgccgt taactgatgc tctggccgga gtgagattgc ccacatctaa caatgaggca
      301 tgcgccgtga caggcagtgg atgagtaagc ggatgcattc tcactccatc gcatggagaa
      361 aacgggtgat tgataaagca atcatcgttc ttggggcgtt aattgcgctg ctggaactga
      421 tccgctttct gcttcagctt ctgaactgat agcggaaacg taattaaggg ctaagagcac
      481 actactctta gccctttaac atttaacgca tggtcacgaa ctcttctgcc gccgttgggt
      541 gaatggcgac ggtattgtcg aagtcttttt tggttgcgcc catcttcagc gccaccgcga
      601 agccctgcaa catttcgtcc ataccaaagc caatgccgtg aataccgaca atcttctctt
      661 ctggcccaac gcacaccagc ttcatgcggc acggctggcg gtgagtggtg acggcggtat
      721 acatcgcggt gaaagaggat ttatacactt tcacctgatc gtcgccgtac tgctcgcgcg
      781 cctgcggttc cgttaaacca acagtaccaa tcggcggatg gctgaagacc acggtcggaa
      841 tgttgctgta atccagatgc tcatccggct tgttattaaa caggcgttca gagagacggc
      901 gacccgctgc aactgccacc ggtgtcagtt ccactgcacc cgtgttatcg cccaccgcgt
      961 aaataccttc aatattggtg ttttgatatt tatcgacgac gatatagcct ttttcgttgg
     1021 ttttaacgcc agcggcttcg aggttgatgt tgtcattggc aggctcgcga ccaatcgccc
     1081 aaatcaggca atccaccgtt tcactgcgac catcttccag ctccagcgtc aggctaccat
     1141 cggcattttt cactaccgct ttcgggatgg cgttggtatg caactgtggg ccttcggcgt
     1201 tcatcacttc gaccagcgtt tcagagatca tcgggtcgaa gctgcgcagc ggcgcatgtt
     1261 tacgcacaaa cagatgcgtt ttcgcgccga ggccgttaat cacgccagcc agctcaacgg
     1321 cgatgtaacc cgcgccaaca accgccacgc gctctggcaa agcaggaagg gcgaagaagc
     1381 catcggaatc aataccgtat tccacgcctg gaatatccgg gtggctcgga cgaccgccgg
     1441 tggcgatcag aatatgatcg gccgtgatgg tttcgccgtt aacttccagc gttttggcat
     1501 caacgaagcg ggcaaagcct ttgattacat caacgttatt tttaccgagc acgttttcat
     1561 aggaagtatg aatacggtcg atataggcgg tacggctggc gatcaacgtt tcccagttga
     1621 acttgttgat ggtggtatcg aaaccataat cagggccgta catatggatc gcttcacgga
     1681 tttgcgccgc gtgccacatc acttttttcg gcacgcagcc gacgttcacg caggtgccgc
     1741 ccagctcttt ggcttcaatc agtgcgcatt tctggccgta catagccgcg cggttgatgg
     1801 aggcgatacc gccgctgccg ccgccgatgg cgatgtaatc atagtgttta gtcatgacat
     1861 attgtcctta tcgttgatta ccgcgattgt agcgcgagac gtaataggtg ccagcaatgg
     1921 ctgcaattac tccggcacga tccagcttac ggtggcgtgc ccggtacctg ccggaaccag
     1981 tttgctgtgc agccacgaca gcacgttatt catctgctgt tccagcttcc acggcgggtt
     2041 aatcacaatc atgccggaag cggtcatgcc acggcgatcg ctgtctggca gcaccgccag
     2101 ttcaatttgc agaattttgc gaatgccggt cgcttccagg tcgtggatca tgcgcttaat
     2161 ttgctggcgc agcaccaccg gataccacaa tgcgtaagtg ccagtggcga aacgtttgta
     2221 accttctgct atcccgctga ccaccgcctg atagtcggtt ttcatttcat acggcgggtc
     2281 gataaggatt aaaccacggc gagaaaccgg cggcagtttg gccttaagtt gctggaaacc
     2341 gtcggctttt tcgacgcgtg cacggctgtc tttctgaaat ccagaacgca gcaacgggta
     2401 atcgctcggg tgcagttcgg tcagttgcag gctgtcttgt tcgcgcagta gctggcgagc
     2461 aatcaacggc gaaccggggt agtaacgcaa ctggccgcta cggttgaagt gttttaccac
     2521 attgatgtac gcctccagtt ctgcgggcaa atcgtcctgc tgccagatac gggcgatacc
     2581 ttcgagatat tcgccggtac gctcggcatg ttcgctacct aactgataac gcccggcccc
     2641 tgcgtgggtg tcgagataga gaaacggttt atctttctct ttcagcgact cgatgatcag
     2701 gctctgaacg gtatgtttaa ggacgtcggc gtggttgcca gcgtgaaagc tgtggcgata
     2761 actgagcatg ggtaaaggtg ttccggtaag taaaaattgg cccgtattcg gggcgcacaa
     2821 aagcctatca ggacagtata ccgaaaagag gccgccgccg cgaaagcgta acgtttctca
     2881 ttgaaattca ctacacttaa ccccatgcta cacacattat gtaaagcgcc tgttgagcgc
     2941 ttccttaacc tctttaacca ggactgcgct aatgacgaat ccgttactga ctccctttga
     3001 attgcctccg ttttctaaaa ttctcccgga acatgtcgtt ccagccgtga ctaaggcatt
     3061 gaacgactgc cgcgaaaatg tggagcgcgt agtagcgcaa ggggcaccgt acacctggga
     3121 aaatctctgc cagccgttgg cggaagtgga cgatgttttg gggcgtatct tctccccggt
     3181 cagccacctg aactcggtga aaaatagccc ggaactgcgt gaagcgtacg aacaaaccct
     3241 gccgctgctg tcggaataca gcacctgggt agggcaacat gaagggctgt ataaagcgta
     3301 ccgcgacctg cgcgatggcg atcattacgc cacgctgaac acggcgcaga agaaagcggt
     3361 tgataacgcc ctgcgtgatt ttgaactctc tggcataggt ctgccgaaag agaaacagca
     3421 gcgttacggc gaaattgcta cccgtctttc tgaactgggc aaccagtaca gcaacaacgt
     3481 cctcgatgcg acgatgggct ggaccaaact cgttaccgac gaagcggggc tggcggggat
     3541 gccagaaagc gcgctggctg cggcaaaagc ccaggccgaa gcgaaagagc tggaaggcta
     3601 cctgctgacg ctggatatcc caagctacct gccggtaatg acctactgcg acaaccaggc
     3661 tctgcgcgaa gagatgtatc gcgcttacag cacccgcgcc tccgatcaag gcccgaatgc
     3721 cggtaagtgg gataacagca aggtgatgga agagatcctc gcgctgcgtc acgaactggc
     3781 gcaactgctg ggctttgaaa actacgcctt taaatccctt gccactaaaa tggcagaaaa
     3841 cccgcagcag gtgctggatt tcttaaccga tctggcaaaa cgcgcgcgtc cgcaaggcga
     3901 aaaagagctg gagcaactgc gtgccttcgc taaagccgaa tttggtgtcg atgagttgca
     3961 gccgtgggat atcgcttact acagcgaaaa acagaaacag cacctctaca gcatcagcga
     4021 cgagcagctg cgtccgtact tcccggaaaa caaagcggtt aacggcctgt ttgaagtggt
     4081 gaaacgtatt tacggcatca ccgctaaaga gcgtaaagat gttgatgtct ggcatccgga
     4141 tgtacgtttc ttcgaactgt atgacgaaaa taacgaactg cgcggcagct tctacctcga
     4201 tctgtatgcc cgtgaaaaca agcgcggcgg ggcgtggatg gatggctgcg taggccagat
     4261 gcgtaaagct gatggttctc tgcaaaaacc ggtcgcgtat ttgacttgta acttcaaccg
     4321 cccggtaaat ggtaaaccgg cgctgttcac tcacgacgaa gtgatcaccc tgttccacga
     4381 gttcggtcac ggcctgcacc atatgctgac ccgcatcgaa accgctggtg tttccggtat
     4441 cagcggtgtg ccgtgggatg cggtcgaact gccgagtcag tttatggaaa actggtgctg
     4501 ggagccggag gcgctggcgt ttatctctgg tcactatgaa accggcgaac cgctgccgaa
     4561 agagttgctg gataaaatgc tggcggcgaa gaactaccag gcggcgctgt ttattctgcg
     4621 tcagctggag ttcggcctgt ttgatttccg ccttcatgcc gagttccgcc cggatcaggg
     4681 ggcaaaaatc ctcgaaactc tggcagaaat caagaaactg gttgccgtgg tgccatctcc
     4741 gtcctggggc cgtttcccgc acgctttcag ccatattttc gccggtggtt atgcggcagg
     4801 ttactacagc tacctgtggg ctgacgtact ggcggcagat gctttctcgc gctttgagga
     4861 agagggcatt ttcaaccgtg aaaccgggca gtcgttcctc gacaacattc tgagccgtgg
     4921 cggttcagaa gagccgatgg atctgttcaa acgcttccgt ggtcgtgaac cgcagctgga
     4981 tgcgatgctg gagcattacg gcattaaggg ctgatcattc agtgaaaatc tgcttaattg
     5041 atgaaacagg cgccggagac ggcgccttat ctgttctggc ggcccgctgg gggctggagc
     5101 acgatgaaga caacctgatg gcgctggtgt taacgccgga acatctggaa ttgcgcaagc
     5161 gtgatgagcc aaaacttggc ggcatctttg ttgattttgt tggcggagcg atggcgcacc
     5221 gacgcaaatt cggcggtggt cgcggtgagg cggtggcgaa agcggtgggc attaaaggcg
     5281 attatttgcc ggatgtggtg gacgccaccg cagggctggg gcgcgatgcc tttgtactgg
     5341 cttcagtcgg ctgccgcgtg cggatgctgg agcgtaatcc agtggttgcc gcgctactcg
     5401 acgacggcct ggcgcgtggt tatgcggatg cggaaatcgg cggctggttg caggagcggt
     5461 tgcagttaat tcacgcctcc agcctgacgg cgctaaccga tattacccca cgcccgcagg
     5521 tggtctatct cgatccgatg ttcccgcata agcagaaaag cgcgctggtg aaaaaagaga
     5581 tgcgtgtctt tcagtcgctg gtggggccgg atcttgatgc cgatggatta ctggagcctg
     5641 cacgcttgct ggcgaccaaa cgcgtggtgg taaagcgccc ggactacgcg ccaccgctgg
     5701 cgaatgtcgc cacgccaaac gcggttgtga ctaaagggca tcgctttgat atctatgcag
     5761 gtacgccagt gtaaaagtaa agcagggtag cgataacgct accctgcttt ctgcaagaat
     5821 aattaatggc tttccggcgt cgcaatcatg cgtttcagcc acggcaccat caacagcatc
     5881 actactgcaa cgcccagcgt gaccagacca atcttaccaa acacgttggt atagacgggc
     5941 aacgtctcaa gcggatcggt aatgttgtcc ggcaccgcgg taaatgttgc cacatagccg
     6001 cccagcaaga acgcggcagc ctgcgtcagg aaccacatcc cgagaataaa gcccatcaaa
     6061 tgctgcggca ccagggcagc aatcatcgcc aggccaaggg cgctaataaa caattcaccc
     6121 aggctctaga ataaatacac cagcacgata aaccatggcg atgtcagccc ctggtcatcc
     6181 gcaaaccaca ttcccgcagc agcagccgtc aaaaagccca gtgagcacat aaacatgctg
     6241 agagtaaatt tcatcggcat cgagaggtct ttgcctttgt tacccagatg cgtgtaaatg
     6301 cctgccagta ttgggctggc gagtaccacc cagaacgggt taagcgcctg gaagctgacc
     6361 gggttgatgg aaaaaccgag aatttcatga tgcacgttgt tgatggcaaa gaagttcagc
     6421 gatgttggca tctgggcgta gagaatgtaa aacaccaccg cttcgagcat caggacaaag
     6481 gcgacaaaca ttttattgcg cccggtttta tccagcttga atgcctgacg aaagaagatg
     6541 atagtgacga cgatggagag aacaatcagc accagattgg cgacttctac gttgtgcatc
     6601 agccatgcac atacgaagat catcaccacg ctgccaagta atacgtacaa cagtttgctg
     6661 aagctcatcg gcttgaagtc gggttcagaa ccaatgtctt tcaccattcc acgacaggcg
     6721 atgtaaacca gtaatgcgat aattaacccc gccccgcaca ggttgtaggt gactgaataa
     6781 ccgaatctat cagcgatcac aggggccagc gataacgcta tcaacgagcc gatattgatc
     6841 gacatataga acagggtgaa tgcgccatca agccgcggat ctttcggcgg atagcacttc
     6901 gaaagcaagc tggctgggtt agctttaaac aggccgttac cgacagcgat agtccccagg
     6961 gcgatgaaaa tcaggtcagg cttaagtagc gacatgccgg tcatgaagta gccaatcgcc
     7021 agcacaagtg ctccaagaac aatggtgcgt ttggtcccca acaggtggtc gccgacatag
     7081 ccgccaatgg aaatgaggcc atagaccagt gcagcaaaag caccaaaagt gacaaaagcc
     7141 tgctcttgcg agaatccaag ctgtttaacg aagaaaaccg ccagtacgcc ctgcacgccg
     7201 tagtagccga atcgctccca taactcgaca aaaaagatca tgaaaaatgg gcgaggttgc
     7261 tgcagcatcc ccatgggtgt tgttgtatta atatttatta accttccaat accatcctga
     7321 aaagtatgaa cgcgtaaggg ataacgcgcg aaaaagttta catctgtcag agattcttaa
     7381 tattgatgcg gtgaatagat taatacacta ttacaacaga aaataaccag ctgatcacac
     7441 taatgatgga aaatatgatg gtgttaaatt ttgtattggc aggagatttt gattgcgtac
     7501 gcagatcgta ggccagataa ggggtttacg ctgcatcagg taacaaggcg cgccaaaaag
     7561 caaaacgccc gcatatgcgg gcgttcagga acacgtcgtg gagggatgat tattcttctt
     7621 cgtcgcgtag cggaacaatc agcatatcaa cgtgaacggt gttgatcagc tgacgtgcgg
     7681 aagacatcag tttgctccag aagtcctggt ggtgaccaca aaccaccaga tccatatcgt
     7741 atttcttgat tgcatcgacc agaacctggc ctaggtcgcc gctgccgctc agggtttcag
     7801 tgattggata gcctgcatta gtggaaagct cggtcagcgc atgatgtgtc tcttcagaga
     7861 tgcgtttctg catatcaccc agattcacat caataagccc ggtgtatagg tcagagtagt
     7921 ttacatctac gtggatcaga gaaactttcg cattgtaggg gcgagccata gagactgctt
     7981 tctctaccag aactttgctt tccggggaga gatcgactgc gatgagaatg tgtttataag
     8041 ccatagtgtt actccttcca taaagttgtc gatgactggc cagctagcgt ttcttgtgcc
     8101 gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct aacccagccg taaagattca
     8161 gtgttaagac catccttacc ttatagcgac ccggatgatc cgtcaatccg ccttgcttac
     8221 ccgttagata aacaaatttt cgacatgaat attatattga tagtggttaa ccttctggaa
     8281 aaaaaacaac ctgatctcct acactatcta tagagccgct cggatgttgg tcaccacaaa
     8341 agcggtttct ccaggacctg gtgttaactg attggcagcg tatcgggagc ggtagccccg
     8401 gggtggaact ccgcgggcag gtcgccgggg aggaggtatg ataagcaccg tcgcattatt
     8461 ttgggcttta tgtgtcgttt gcattgttaa catggcgcgc tatttctcat cactacgcgc
     8521 gttgttagtg gtactgcgta actgcgatcc attgctctat caatatgttg atggaggggg
     8581 cttttttacc tcacatggcc aacccaacaa acaggtgcgt ctcgtttggt atatctatgc
     8641 ccaacgttat cgcgatcatc acgatgatga gtttattcgc cgctgtgagc gggtgcgtcg
     8701 gcagtttatt ctgactagcg cattgtgtgg tctggtggtg gtcagcctga ttgcattgat
     8761 gatttggcat taagcacttc agcaataata acgtataaaa aaagcgggcc agtaaactga
     8821 cccgctcgtt ttggtgcgta cgattacagg aactgcaagg agaaccagta cagcccgccg
     8881 gaaagtagta ccgcagccgg aagggtaaac acccaggcca tcagaatact ggttacggtt
     8941 ttacgctgta agccgccgcc atctaccacc atcgtccccg cgacagaaga ggagagtacg
     9001 tgagtggtgg aaaccggcat cccggtataa ctcgccaggc cgattgacac tgccgccgtc
     9061 atctgggcag acatcccctg agcgtaggtc atgcctttct taccgatttt ctcaccgata
     9121 gtcgttgcca cacgacgcca gccaatcatc gtaccgatac ctaacgccag cgcgaccgcc
     9181 atgatgatcc acaccggcgc atactcgatg gtgctaagca tgtcggactt cagtttcttc
     9241 aacaggcgct gatcgtcagc actcacacca ggcatcttca ccactttgtc gatagtgtca
     9301 gaaacgcaca gcataatgcg gcgcatctgg ctacgttgat caagcgacag cttgtcgtag
     9361 ctttccacat cggtggtcaa catacctttc aggcggttga gcgcgttaat ggtattcgac
     9421 ggatggcagt ggaactccgc aggttgcgtt gcgccagctt ccggagccgg tactaactga
     9481 tcagcaccgg tagcctgttt gagcagcgca ggatgctgct caaagtaagc ttcgacgttg
     9541 ttgatggcat cacgggtacg ggtgatttcg tagccagtgg cattcatgtt caccacgaag
     9601 cctgctggcg cgacgccaat caataccaac ataaccagac caatgccttt ctgaccatcg
     9661 ttcgcgccgt gcgaaaacgc cacgccgata gcggaaagga tcagcgcgat acgcgtccag
     9721 aacggcggct ttttcttgcc gtctttcttt tcacgctccg ctggggtcag gtggatacgg
     9781 gcgcgtttct tggtgccgct ccagtagcga cgcagcaaga aaatcagacc gccagcaaac
     9841 accaggccga caatagggga aacgatcaga gaaccgaaaa tacttaatac tttcgggata
     9901 ttgagtgcat ccaccactga cgtcccggtc atcaacgcat tggttaaacc aatcccgatg
     9961 atcgcgccaa tcagcgtatg agagctggat gcaggtaaac caaagtacca ggtacccagg
    10021 ttccagataa tcgccgccag caacatagag aacaccatgg caaggccatg agacgatccc
    10081 atattaagca gcagatccgt cggcagcata tgcacaatgg cataggcaac actcagacca
    10141 cccagcaaaa cacccaaaaa gttgaacacc gccgccataa ccacggcgag ctgagaacgc
    10201 atcgcgcggg tatagataac ggttgccacg gcgttggctg tgtcatggaa accattgatg
    10261 gcttcgtaga acagcacaaa agccagtgca agcaataata acagcccggt atgcaaatcc
    10321 aggccagcaa acaaatgtag cataggacgt tacgccattt tgaggacatg aacgcggcgc
    10381 attatcagtg actttcgcgg cgcgggcaaa gtgaaatata gacttttttt gatttgcctc
    10441 ctgtatggat ttcactcaaa aaataattat cttatataat tcaggcaaat acttcctttt
    10501 agtaatattg atgctggtgc gaccactgag gaatctttac aattcacgcc cgttttttct
    10561 aagaggagcg caacgtggaa aggtttgatg ccattat
//
LOCUS       DAJTYM010000121        10447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTYM010000121 DAJTYM010000000
VERSION     DAJTYM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..730)
                     /gene="rspA"
                     /locus_tag="OO575_003391"
     CDS             complement(<1..730)
                     /gene="rspA"
                     /locus_tag="OO575_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6666233.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPA"
     gene            complement(936..1262)
                     /locus_tag="OO575_003392"
     CDS             complement(936..1262)
                     /locus_tag="OO575_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR6666234.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1397..1738
                     /locus_tag="OO575_003393"
     CDS             1397..1738
                     /locus_tag="OO575_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6666235.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1804..2333
                     /gene="speG"
                     /locus_tag="OO575_003394"
                     /pseudo
     CDS             1804..2333
                     /gene="speG"
                     /locus_tag="OO575_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2336..3046)
                     /locus_tag="OO575_003395"
     CDS             complement(2336..3046)
                     /locus_tag="OO575_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6666236.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3154..3459
                     /locus_tag="OO575_003396"
     CDS             3154..3459
                     /locus_tag="OO575_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6666237.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3658..3978
                     /locus_tag="OO575_003397"
     CDS             3658..3978
                     /locus_tag="OO575_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR6666238.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            4538..5116
                     /locus_tag="OO575_003398"
                     /pseudo
     CDS             4538..5116
                     /locus_tag="OO575_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5802..6791)
                     /locus_tag="OO575_003399"
     CDS             complement(5802..6791)
                     /locus_tag="OO575_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666239.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(6876..7364)
                     /locus_tag="OO575_003400"
     CDS             complement(6876..7364)
                     /locus_tag="OO575_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666240.1"
                     /translation="MMFNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLSG
                     LPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAER
                     MREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWMD
                     SL"
     gene            complement(7685..8185)
                     /locus_tag="OO575_003401"
     CDS             complement(7685..8185)
                     /locus_tag="OO575_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666241.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8175..8690)
                     /locus_tag="OO575_003402"
     CDS             complement(8175..8690)
                     /locus_tag="OO575_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666242.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9121..9489)
                     /locus_tag="OO575_003403"
     CDS             complement(9121..9489)
                     /locus_tag="OO575_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666243.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(9901..>10447)
                     /locus_tag="OO575_003404"
     CDS             complement(9901..>10447)
                     /locus_tag="OO575_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6666244.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2692 a         2662 c         2572 g         2521 t
ORIGIN      
        1 ccgctggcgt cgggtcttcc atccagaaca tgcgataatc ttcaatgctt ttaccaaagc
       61 gcgccgcttc aataggcgtt aagcgatggt gcatgtcatg cagcaaatgt tcattaaaac
      121 caaacttgtt acgtaccgcg tcaaacaatt tcggcatgaa atcgaggtat ttctccgtcg
      181 accacagctg ctcttccggc cactgtcctt tggttgcggg ttcataagcc agacctttac
      241 ctttcgacat gccgtaggtg gttttcatac cagggattcc gcactgcacg cggatggctt
      301 tgaatcccag ctcctgatga cgggcataat catccagagc ttcatcaata ctgtgaccgg
      361 tggtatggca ataaaccatc accccttcac gagacgcgcc gccgagtaac tggtaaagcg
      421 gcatgttggc agctttggct ttaatatccc acagcgccat atcaaccgct gaaatggccg
      481 acatcgtaac cggaccgcga cgccagtaag cacctttata gaaaaactgc cagatatctt
      541 cgatacggtg cgcatcgcga ccaataagcc gcggacaaag atgatcctgc aaataagagg
      601 ccacggaaag ctcacgtcca ttgagggtgg catccccaag gcccgtaata ccgtcctcag
      661 tggtgatttt taatgtgacg aaattacgcc ccggacaggt aacaaaaact tcagccttta
      721 cgatcttcat gttcgattcc ttgcatcgct tgtcgtgatg catgaaatct acgcaactga
      781 gctactgcca tacaagtatg aagatcgaaa aaagccggag tgatcacaaa aaaaggcgta
      841 tatttgcgct gtgaatggtt gacaaaagat gaaatagaat accttttgtc agctgacact
      901 tcctcgtatc ttattgataa aatggattta tgttcctacg tgcgccccca gcccgcaaca
      961 atgatcaaca ttccgcaaag cgcaatcaac gcacccgtcc agtcataaag acttagtttc
     1021 acgccatcca caacgcgcag ccacatcaat gccgtgcaga cataaacgcc accataagcc
     1081 gcgtaaacac gcccactcgc cgcaggatgc aacgttaaca accagacaaa cagcgccagt
     1141 gaaatccccg ccggaagcaa cagccagata ctggcgtttc gttttaacca caaccagggc
     1201 agaaagcatc caataatttc acacagcgca gtagcaaaaa atagtaacgt tgttttaatc
     1261 atctttgtca cttattgaca tcatgtatag ttatagggcg acataatatc atcaatataa
     1321 acatcctcct ggtacgtttt gcgttcgcag tggatggtgt agaatcacct ttaatcattc
     1381 atacagggaa tgaattatga aaatcactct cagcaaacga atcggcctgc tcgcttttct
     1441 gctgccttgc gcactggcat tgagcacaac tgttcatgcc gaaactaaca aactggtgat
     1501 tgagtctggc gacagtgcac aaagccgcca gcacgccgct atggaaaaag agcaatggaa
     1561 tgacacgcgc aatctgcgcc agaaagtgaa taaacgcact gaaaaagagt gggataaagc
     1621 cgacgccgct tttgataacc gcgataaatg tgagcaaagt gccaacatca atgcctactg
     1681 ggagcccaat actttgcgct gcctggaccg tcgaactggc cgcgttatta ccccctaacc
     1741 tgttattgat ttaaggaatg taaggacacg ttatgccaag cgcccacagt taagctacgc
     1801 ccgctggagc gtgaagattt acgctatgta catcaactcg acaataacgc cagtgtgatg
     1861 cgttactggt ttgaggaacc ctacgaagcc tttgttgaac tctctgatct gtatgataag
     1921 cgcatattca cgatcagagc gaacggcgct ttgtggtgga atgtgacggc gaaaaagccg
     1981 gtctggtgga gctggtggaa attaaccatg ttcatcgccg ggcagaattt cagataatta
     2041 tctccccgga gtatcagggg aaaggtctgg caacccgtgc cgccaaatta gcaatggact
     2101 atggcttcac cgttctcaat ctctataagc tgtatctgat cgtcgataaa gagaatgaaa
     2161 aagcgattca catttaccgc aagcttggct ttacggttga aggtgaattg atgcatgagt
     2221 tttttattaa tggtcaatat cgtaatgcca ttcgcatgtg tatattccag catcagtatc
     2281 tggcagagca caaaacaccg ggtcagactc tcctgaagcc gaccgcacaa tagcattaat
     2341 aataatcgat cgtatttttg atggtgtaaa cccgttcgac ggcgggtttt actccttcat
     2401 caacaatgat cagctgacag tccactggat tggcgtgact gtcatattca cagctctgtt
     2461 ttacattacc aactcgttga ttatttagta aagtaaccgc tgtgtaatct aattttttga
     2521 tcggatccgt tgatggcgtg gcgctgacag ataatgtttt gtcgttactt ttcgtggttt
     2581 tacccagcgg atacccctga tcatcatagc gatattccat ctgcatttgt ttgctgctgg
     2641 ctttaatcac gaagccatta tcatcggttt cccaactcac cccggcagaa ggtaattctg
     2701 ctagctggca ttttccctgt aaacgtactc tcttctccag cgtctcggca tcacggtaat
     2761 aattggcgtc cagtaccaga gcgaccacgg tattattttc cagatccagt aattcgagtg
     2821 aatcaaaaca gccttcttcc gacaaagtcc cagaaacacg tttcgtcact tcaccttgct
     2881 catccattaa tgtctgagtg aaatctttta ccggaccacg cagcggatca aaatcgaatt
     2941 cattagagaa actggccatc tcaggggtaa atgaaagtgt tacctctgtg cggtcacatc
     3001 ctgtgaggaa tatcgcgagt aagcatggta gtaatttgta tttcacaaca gtcaccagag
     3061 agtagagatg attctcaatc atagtagcaa atacagtact ttacacgtta aatgctatgc
     3121 ttaaagaagt tatcttcgcg taaggagctt atgatgaaac tctcaacctg ctgtgccgcg
     3181 cttctgctcg ccctcgcctc acccgcggta ctcgccgcgc cgggatcttg tgagcgcata
     3241 cagagcgata tatcacagcg cattatcaat aatggcgtac ccgaaagcag cttcacgtta
     3301 agtattgtac ccaatgacca ggttgatcag cctgattccc aggtcgtcgg gcattgcgct
     3361 aatgatacgc ataaaattct ctacaaccgc accaccagcg gtaacgtctc tgctcccgcg
     3421 cagtctaccc aggatggcgc gcctgccgaa ccgcagtaac acattatcga ctgaacgccg
     3481 gatatgacac atccggcgat ttgaacatac aacataatct caccttatta ctcataccct
     3541 tctattgatg tggattaata attcttaacc caaaatgggt agactccctc tattgttagc
     3601 gcgctaaata ttcaatgtat aaacatttat ataacaataa agaacaggga gtgagttatg
     3661 tccaaaaatg accgaatggt gggcatcagc cgcagaacac tcgttaaatc aaccgcgaca
     3721 ggttctctgg cgctggctgc aggcggtttt tctttaccgt tcaccctgcg cagtgcagcg
     3781 gcagcggtac aacaggccag cgaaaaagtg atctggggtt cctgttccgt caactgtggt
     3841 agccgctgtg cacttcgtct acatgttaaa gataatgaag tgacctgggt ggaaactgac
     3901 aataccggca gcgatgagta cggcaaccat cagggtacgc gcctgtttgc gcggtcgctc
     3961 catccgccgg cgtattaatc atcccgatcg cttgaatgac ccaatgaaac gcgtgggtaa
     4021 acgcggtgaa ggcaaattcg aacggattag ctgggatgaa gccctggata ctatcgccag
     4081 tagcctgaaa aaaaccgtcg aacaatatgg caatgaagct gtatatattc agtactcttc
     4141 ggggattgtt ggcggcaata tgacccgctc ttcgccatca gcctcggcgg tcaaacgcct
     4201 gatgaactgc tacggcggtt cactcaatca gtatggctcc tacagcaccg cgcaaatttc
     4261 ctgtgccatg ccctacacct acggcagtaa tgatggcaac agcaccacgg atattgaaaa
     4321 cagcaagctg gtcgtgatgt ttggtaacaa cccggcagaa acccgcatga gcggtggtgg
     4381 catcacttat cttcttgaaa aagcgcgcga gaaatcgaac gccaaaatga ttgttatcga
     4441 tccgcgatat accgatacgg ctgccggtcg tgaagaggaa tggctcccta ttcgcccggg
     4501 caccgatgcc gcgctggttg cgggtattgc ctgggtattg attaacgaaa atctcgttga
     4561 tcaacctttt ctcgataaat actgcgtcgg ctatgacgaa aaaaccttac ccgcagatgc
     4621 acccaaaaat ggtcactata aagcctatat tcttggtgaa ggtgacgata aaacagcaaa
     4681 aacgccgcag tgggcttcgc aaattaccgg tatcccggta gaccgtatca tcaaactggc
     4741 gcgtgaaatt ggcacaacaa aacccgctta tatctgctag ggctgggggc cgcaacgcca
     4801 ggcaaacggc gagctgactg cacgcgctat tgctatgtta cctattttga cgggcaatgt
     4861 cggcatcagc ggcggaaata gtggcgcgcg tgaatcgacc tataccatta cgatagaacg
     4921 cctaccggtg ctggataatc cggtcaaaac gtcaatctcc tgcttcagct ggacggatgc
     4981 tatcgatcat ggcccgcaaa tgacggcact ccgcgacggt gtccggggca aagataaact
     5041 ggatgtgccc attaagttca tctggaacta caaggacttg aagcggaccc ttagtaggcc
     5101 tgaggtcctg cattgagtca agaccttgtg tcattctttg tgccagactt gtgtcattag
     5161 acgggatgtc tagacggctg gtcaggaccc tccggtggcc tcggtctgga cttgaggcgg
     5221 gctatggggg aagtctgctg gcttaatgta ttagatggtc attcacattt ttctgtcaga
     5281 aaccatcggt cccactgtga gtacaaacct aagcccccag aagcccaccc acagccttag
     5341 acagagtgtc gaatacattc acaccgagtc gaccggagag gaccatatag aggcccacag
     5401 agaggaacac gagtttatag accagagtca ggagggacct taggtcttcg gaagtcatgg
     5461 acataaagcc cccccctatc ttctattgca ctaccacact agacaactag tacaaaacaa
     5521 aggcccagac cattggtcca gaccgtagtt agttaatcac agtgacaagc caccacctga
     5581 agtcctgctg atattcagtc cagacttacc tctgctcatg gtcttacctc cctcggtctc
     5641 ctgaccttgg gtatcgacta cagcttgctg agcgtcatct ggaggagcta caggaggagc
     5701 tacaggaggc gggaccgaag ccccaccttg agtcccacca ccacctgtca ggcccttggc
     5761 gatggacctg aagcctttac taatgctctt acccattggt cttacgcttc ggtcttggtg
     5821 aacttcaggc gtttgactac aggcaggacg gcagtagctg agccagtgcc catcttgatg
     5881 gtcgtagcag cttcagcgac aacgaacact ttaccgagga tttccaagtc gtgggactga
     5941 gagtcgccat cacgtgaaag gacgaccgta atattctcac cgtcgtgacg gaagcagccg
     6001 aagctcaggg ccgtgaagcg tttagccagc atgaatacac ccttgtcttc accgttgtaa
     6061 taggcgatgt ctgtccccag catatcgacg atagcgtctt caccggagaa gccttgctta
     6121 gcagccagac gggacagtgc tttcagttgg ctctcatgag cgataatacc gtagtcagac
     6181 acctcagaac cgaaagaaga ctctgtgttc tcatacagag cgtccatgat agcgtctgcc
     6241 ttagcggtca ggctaccagt caggacagga agctctacgg tcttcagcag cttagaggat
     6301 tccagaacgt cgaccatgcg agcgttgatg gtacggacca cattctcaca gatgttcata
     6361 gcgtcctgct gaagtgtgcc ctgagtgaat ccggtggatg acttggcgtc ccagagcttg
     6421 gtctgctcag agatgagaat agcctgctcg attggttcga ccacgatttc ttgtccagac
     6481 ggaacggtca cgacttcagg tcgcttgcca ccacggacag gaacgaccac agagcggtca
     6541 gccagagtgt gagcaatgat tggggtcggt aagctgttac gggtcggcag gacttctaca
     6601 gggaacggga tgttctcttg agcgtcttta agtacaccgt tcagcatagc cttaaccgtc
     6661 ttagctaact ggagggcata gacctgcggg tcagcgttag tcggcagacc ttcattgacc
     6721 tgaatgttgt cggtatcggt gatgcgtact ggctcaatgc caaactggaa agcgtcttta
     6781 atcgtagtca taataatcaa tctcctaaat tagtttaaat actgtgagtc ctcaaaactg
     6841 aggagtccct aacatcgggt cagggactgt gtgttttaca agctgtccat ccagttaccg
     6901 cctgactcaa ggtcagcgac aatagcggca cgggagcgac cagcggaatg ggtagcactg
     6961 cggaggctct caccataggt ccggcgttca gctttgacct gttcgcggcg ctgtagctct
     7021 ttctgccact ctgcatcggt catgttctct tcacgcatac gctcagcctg aagtcgttgc
     7081 tctttagcga tacgctcagc ttcagctcgt ttgttcatct ggcggcgacc ataggcggtc
     7141 atcaagtcat tactgaagaa gttgccaatg gtcacgccag cttcagccag agcttccagt
     7201 aactccattg cctcgcggct ggtagacggc aggcctgaca ggtagcccgg aatgagggtc
     7261 ttgatagcga gcgacagcgg atagggctga ccgtccagac tcacagtgtt ttcataaagg
     7321 ttgatgtcgg tgactgacag cttccagttg ttgttgaaca tcataggtaa ttctcctctt
     7381 gagagttgag actatcggta tcgcagacgc agtagcgcct acgggtctcc ccgcatcggt
     7441 catctcaaaa ggtttaaaat taggtttggt atggtcagga ctgcttagca aaggctcggc
     7501 gtaactgatt gtctaaatca cggtccacca gcatagctat cagctcgtct cgactgtagt
     7561 aagggcgctc agggtcttcg actccaagtc tcagcagaga cagcagatga cgtgtgggca
     7621 ggccacggta acgaagggtc gctttgccga taggtgtacg agggtcaatc atagccatga
     7681 ttacttacct ccactcacag cgaccagcgg tcacagcttg atagtctgaa tactcagcct
     7741 gtgagacttc acgtccggcg atgtagaggc ctgtgccatg acgctggatg aattccttgg
     7801 tctccggctt gatgtcctca gcagacgcta cagcagactg cacggtggcg ctggaggtca
     7861 tagacacgaa ggctcgggtt actgccagtc cgtacccaag gtccaaccac cgaccgaact
     7921 ctatagcggc tactgggtca gccagccacg aggtccctaa gtgacccatg ttaatctttt
     7981 taacaaaccc gttttctggg ttggttagac ttctaccagc cttggtcctc atgaagcgag
     8041 caacctcggt actacggtta tcttctacct taccaatctg aatgcctacc cggcgtatct
     8101 catccagtcg aaacaatccg tcctgactgg tgatgtcctg accgaagata tgaatggtgt
     8161 attcctgctt attgtcagaa gtcataagag gcacctcccg ccagagcttc ggcaaaggac
     8221 ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt cggcatcagg tgggaccagc
     8281 ttatcaaagg tgccgttttc gtaatcttca gttaggacct gaaggcggcg ggaccatttg
     8341 ctctccacct gcatcatcgc gttagagatg tgcttcaagt cggcatcaga gcgtgacgct
     8401 atccaatcca tctcttctga gtcaccctca caggtgtaag gattgttgaa atagaagatg
     8461 tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct ggaggtatga ctggataggc
     8521 tccgcgtaca tctcccggat tttctcatag tcccagtctt ccgcctgggc ataccatcgg
     8581 gataacttca gcaggtctaa gttatagcga cagcaatcga cactggactc tgcattggtc
     8641 agcaggatga ttaagaactg gcggttagtt actggtaatt cggtattcat acaaagatgc
     8701 tcctttggta aagcactttc tgtctccaca tggagtccgc accattcaga cgcatcctaa
     8761 acggacccat cagaaagtgc ttaacgaaag tgaatggtta aatgttgacg gtctggagtg
     8821 accatgttct ccatgtacgt ataaaggtgc gaccgcatgt caggacgagc tgtaaggact
     8881 ccagatggtg agcggtgata gacttaagcc aatgtagtga gtagacgagc tactaaccga
     8941 gacgactaaa gaacgctaac gagctggagg tcgatacagg aggtctgtca gaggcggtca
     9001 cgggccttga ttacttagga gagatactta atgtcggttg accagatgct cgcatgttct
     9061 gacgcatcag actcagaggc ggaggctggt ctccctatag tgatagttta gggtccgtaa
     9121 tcatgaacca gctttactgg cgtggtactg gacggattta ctggcgctga cgacggtagc
     9181 atagacgtag cgagcgtagc gagttgaaac cataggttca cccaaggcaa cacgcttacg
     9241 gctcagggac tcctgcacag cgcttacagt taatgtgtcg tgcgttagta gttgggtcag
     9301 aatcaagtat gggctgacag gcttggtatt gcttgccatt gattcgttca ggcgaccagc
     9361 accagtaagg acatcatcca gccaaccact caggtcgccc tgaccatcga atgacagcag
     9421 gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg ttgtcgtgag ttgcgattgg
     9481 tcgtgccata gagtatctcc tgtagttgaa ttagttggtt cccctatagt gataatcaag
     9541 tccatcactg gtgatgcata cagtagcgcg acaggatgtg actcacaggt tgaaccgaat
     9601 gtcaaggact aaagacgcct caggaattaa ggaagtatat ataaagagag accagaggcg
     9661 actcacagtt aaacaggaat gtcaagagga gaccatagga atttggaggg actggagttt
     9721 aggacgcagt ttaaataaga ccacagacag acttagagtc ggacagccga aggcggacca
     9781 gtgggcctat agtcaggagg gattatgatt attatcaccc tccctaactt cagtcagact
     9841 tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc tccaccttgg gtctccctac
     9901 ttaaagtaaa gctgtcttaa gcacatcagc cagcctaccc acagcgtgtc ctttcccgta
     9961 caggtcatac gtaattgact gactgctgtg acctaagatg ccttgagcgt ccaccagatt
    10021 gactccacgc tctttcattc gggtcgccag ccaatgtctc agactgtgga gagagacatc
    10081 ctgagagtct cctagggcct cagtcaggac ccttgagtta aaccagctag agtaggcgct
    10141 cggagtcatc ccgaagagag gaccctcatc aggctgcatg tctacccacg acaggaagga
    10201 cgtcaggtca aacccataag cgccatcggt taacggcaca agtcggactg aatgtttgtt
    10261 cttcacagac ttcccgtccc catcttcatt gatgtcgata cagaccagtc cattgtccaa
    10321 ggttacaatg tcacgtttag tcaggtggca gacctcagca gaccgagcgc cagtgaccac
    10381 agacaccaga gacgcccatc tacgggcctc agaggtcgtg tgagccgtgt gtcgtgtgaa
    10441 ctgatac
//
LOCUS       DAJTYM010000122        10372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTYM010000122 DAJTYM010000000
VERSION     DAJTYM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..272)
                     /locus_tag="OO575_003405"
     CDS             complement(<1..272)
                     /locus_tag="OO575_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6666245.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="OO575_003406"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="OO575_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="OO575_003407"
     CDS             complement(1132..1338)
                     /locus_tag="OO575_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6666246.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="OO575_003408"
     CDS             complement(1445..2008)
                     /locus_tag="OO575_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6666247.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="OO575_003409"
     CDS             complement(3383..5218)
                     /locus_tag="OO575_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666248.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="OO575_003410"
     CDS             5319..5606
                     /locus_tag="OO575_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6666249.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="OO575_003411"
     CDS             5578..7107
                     /locus_tag="OO575_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6666250.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="OO575_003412"
     CDS             complement(7277..8494)
                     /locus_tag="OO575_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6666251.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="OO575_003413"
     CDS             complement(8861..9847)
                     /locus_tag="OO575_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666252.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..10372)
                     /locus_tag="OO575_003414"
                     /pseudo
     CDS             complement(9844..10372)
                     /locus_tag="OO575_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3231 a         2371 c         1949 g         2821 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgt aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataaccc
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcagcg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     9961 gaaagctacc ggggttggca tattcagaat atgtatcgag agattcagtc catttcaccg
    10021 tatgagttaa gtgtaatgag agattttctt tgataattga cggagcgacc tcgctttttg
    10081 ctgttacgtt catcagaacc tgatgctctg gcgaagcgat atccagtccg gcaagataat
    10141 cgcgcattgc ctgaacgccg tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga
    10201 gtttaaccgg ttcgtatcca gcggccatca gcagcgtggt aaattcattc aacgcagcct
    10261 gcgttccccc aataacctgc tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc
    10321 ctgattccgt aatcatcgtc tgcagttgtt cgcggttgat gcctttaacc gc
//
LOCUS       DAJTYM010000123        10094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTYM010000123 DAJTYM010000000
VERSION     DAJTYM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            123..254
                     /locus_tag="OO575_003415"
     CDS             123..254
                     /locus_tag="OO575_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6666253.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            514..1509
                     /gene="pgl"
                     /locus_tag="OO575_003416"
     CDS             514..1509
                     /gene="pgl"
                     /locus_tag="OO575_003416"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6666254.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1550..2503)
                     /locus_tag="OO575_003417"
                     /pseudo
     CDS             complement(1550..2503)
                     /locus_tag="OO575_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2687..3739
                     /locus_tag="OO575_003418"
     CDS             2687..3739
                     /locus_tag="OO575_003418"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6666255.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3815..5248
                     /locus_tag="OO575_003419"
     CDS             3815..5248
                     /locus_tag="OO575_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6666256.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5431..7611
                     /locus_tag="OO575_003420"
     CDS             5431..7611
                     /locus_tag="OO575_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6666257.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7752..9035)
                     /locus_tag="OO575_003421"
     CDS             complement(7752..9035)
                     /locus_tag="OO575_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6666258.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9170..10051)
                     /locus_tag="OO575_003422"
     CDS             complement(9170..10051)
                     /locus_tag="OO575_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6666259.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2448 a         2357 c         2550 g         2739 t
ORIGIN      
        1 tttttttctt taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag
       61 gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga
      121 ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta
      181 atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt tggccagaag
      241 ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg gagagtcata
      301 ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg tgactaatat
      361 tttagtaata tgccattcga aatggagtgc taacttattg atttaattga tgtgcgatat
      421 tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta tatgtatgaa
      481 agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta tatcgccagc
      541 cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca
      601 caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat
      661 ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc cccggacgat
      721 ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca tatttccacc
      781 gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg
      841 cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct ggacggttgc
      901 cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat
      961 cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga ccctgcggaa
     1021 gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat
     1081 gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac
     1141 ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt
     1201 tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc
     1261 gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc
     1321 ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg caagtatctg
     1381 attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcagggg
     1441 ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt ggtggttaac
     1501 gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat taacttatct
     1561 cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag
     1621 tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc acggcatctt
     1681 tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg ataccaataa
     1741 tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcctg
     1801 cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc
     1861 agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc
     1921 caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg gccaatggat
     1981 ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg
     2041 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
     2101 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
     2161 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaatgtca
     2221 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
     2281 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
     2341 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
     2401 ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa ctggactctg
     2461 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
     2521 gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag ccaacttaaa
     2581 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgtccttaat
     2641 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
     2701 ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg aacatttacc
     2761 cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg gtaacgatct
     2821 ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca ttattagccg
     2881 ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg tccatgagca
     2941 acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg cattcgccat
     3001 tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc gcaacgtcaa
     3061 tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg
     3121 tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa
     3181 tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg attattttga
     3241 cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata
     3301 tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc
     3361 ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa
     3421 tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt
     3481 gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat
     3541 ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata
     3601 tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg
     3661 tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa tattttccgg
     3721 tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa taaaggactt
     3781 ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct
     3841 aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag
     3901 cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc tggttatcaa
     3961 gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg tggtggtcgg
     4021 taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac
     4081 tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg
     4141 taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg gtctgggtta
     4201 cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc
     4261 gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga
     4321 aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac
     4381 caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga aaatgatcaa
     4441 cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg gattgccggg
     4501 catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac cagaaattaa
     4561 aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac cgatgaaaat
     4621 ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa
     4681 gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct
     4741 gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata ccttaatctg
     4801 gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt tcgaatggtt
     4861 agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt
     4921 tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt
     4981 tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac
     5041 cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact atggcggcgc
     5101 ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt ggttggtcgg
     5161 tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt ggtggtggaa
     5221 tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt tgcatgtgct
     5281 gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ttcactcact
     5341 tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct ttctgcgcac
     5401 ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa aggcgtgttt
     5461 ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat taaaaaagaa
     5521 gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat
     5581 atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt
     5641 attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc
     5701 aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt
     5761 ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc
     5821 catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg gtcagacagc
     5881 cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta
     5941 aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg
     6001 accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg
     6061 gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc
     6121 gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta
     6181 agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggt
     6241 caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt
     6301 gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa
     6361 atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa
     6421 cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg
     6481 aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg
     6541 gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcggtttac
     6601 ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt
     6661 ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc gggcgatacg
     6721 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
     6781 tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
     6841 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
     6901 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
     6961 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
     7021 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtcacc
     7081 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
     7141 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
     7201 caggagcata ttgatccgct gcaaactgaa attggtagca tggtctatgc ggtgaaacca
     7261 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
     7321 aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
     7381 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
     7441 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
     7501 gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga agagcgcgag
     7561 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
     7621 tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc atcttatccg
     7681 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
     7741 gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc gcggttattg
     7801 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
     7861 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
     7921 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
     7981 ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga agcattgaaa
     8041 cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg tgcaaacaca
     8101 tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
     8161 gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt caccagcgta
     8221 cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
     8281 ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc agtacgcagt
     8341 gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
     8401 ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag aacaccgata
     8461 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
     8521 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg
     8581 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt gattccacct
     8641 ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc aatatactgg
     8701 cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
     8761 acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg
     8821 gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg cgcatcgaaa
     8881 ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc agtttgatca
     8941 gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc aaaagccaat
     9001 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
     9061 aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc
     9121 ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat tatttgattt
     9181 caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     9241 tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     9301 acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga cctgatgctt
     9361 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca cgacgagtag
     9421 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     9481 atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc
     9541 tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac
     9601 caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa catggtgatg
     9661 attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat ctccttacct
     9721 ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct tcagctattg
     9781 cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgatgcc gccttgccct
     9841 cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct tcaagtggcg
     9901 catcaggcag ccccctcctt attgctttaa ttttgctcat gtaatttatg agtgtcttct
     9961 gcttgattcc tctgctggcg aggatttttt cgtagcgatc aagccatgaa tgtaacgtaa
    10021 cagaattatc actgttgatt ctcgctgtca gaggcttgtg tttgtgtcct gaaaataact
    10081 caatgttggc ctgt
//
LOCUS       DAJTYM010000124         9974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTYM010000124 DAJTYM010000000
VERSION     DAJTYM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            132..482
                     /locus_tag="OO575_003423"
     CDS             132..482
                     /locus_tag="OO575_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6666260.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            535..930
                     /locus_tag="OO575_003424"
     CDS             535..930
                     /locus_tag="OO575_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6666261.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            971..1714
                     /gene="cmoA"
                     /locus_tag="OO575_003425"
     CDS             971..1714
                     /gene="cmoA"
                     /locus_tag="OO575_003425"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6666262.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1711..2682
                     /gene="cmoB"
                     /locus_tag="OO575_003426"
     CDS             1711..2682
                     /gene="cmoB"
                     /locus_tag="OO575_003426"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6666263.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2847..5193)
                     /gene="torZ"
                     /locus_tag="OO575_003427"
                     /pseudo
     CDS             complement(2847..5193)
                     /gene="torZ"
                     /locus_tag="OO575_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(5218..6318)
                     /locus_tag="OO575_003428"
     CDS             complement(5218..6318)
                     /locus_tag="OO575_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6666264.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6706..7452)
                     /gene="cutC"
                     /locus_tag="OO575_003429"
     CDS             complement(6706..7452)
                     /gene="cutC"
                     /locus_tag="OO575_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6666265.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7466..8032)
                     /locus_tag="OO575_003430"
     CDS             complement(7466..8032)
                     /locus_tag="OO575_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6666266.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8248..>9974
                     /gene="argS"
                     /locus_tag="OO575_003431"
     CDS             8248..>9974
                     /gene="argS"
                     /locus_tag="OO575_003431"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6666267.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVER"
BASE COUNT         2378 a         2598 c         2411 g         2587 t
ORIGIN      
        1 tttattgctt gcatagtaga atctaaccat ccatcactca tagctaaccc tcggaaatct
       61 tacatttctg tctatcataa ccactagcat cacctttagg aaccatctga aacgggaggt
      121 gtatttagac cttgctaatc ttagacagcc gcccggttca tgcatcacgt ccacacagtg
      181 aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg
      241 cgacaaatcc gctgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat
      301 ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt
      361 tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac
      421 gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct
      481 gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta
      541 agcgcgctat atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt
      601 cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg gttttagcga actgcaaagc
      661 gctatttgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt gctgatgctg
      721 tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt
      781 gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgtttcgctg gcgacgttcc
      841 ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg
      901 ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga
      961 tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga
     1021 ctggaccttt gatgaacggg tcgctgaagt cttcccggat atgatccagc gttccgttcc
     1081 cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg
     1141 tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa
     1201 cattcatcat gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg
     1261 ctgccgtcgt catattgacg cctataaagc ccctacgcca gtagacgtca ttgaaggtga
     1321 tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt
     1381 cctggaacct tccgagcgcc aggcattact ggataaaatt tatcaaggac tgaacccggg
     1441 cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaactgtt
     1501 gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca
     1561 gaagcgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg
     1621 cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc
     1681 actggtggca ttaaaagcag aggacgctgc atgatcgact ttggtaactt ttattctctg
     1741 attgccaaaa atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg
     1801 cagcgcgagc agcagcacgg gctgtttaag cagtggtcca acacggtgga atttctgcct
     1861 gaaattaaac cgtatcgtct ggatttattg catagcgtaa ccgccgaaag cgaagagcca
     1921 ctgagcaccg ggcaaattaa acgcattgaa acgcttatgc ggaacctgat gccatggcgc
     1981 aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc cgactggaaa
     2041 tgggatcgtg ttctgcccca tctttctgat ttaaccggac gcaccattct ggatgtcggc
     2101 tgcggcagcg gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg
     2161 atcgacccta cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggtaac
     2221 gatcagcgcg cacatttgtt accgttaggt attgaacagc ttccggcact gaaagccttt
     2281 gataccgtct tttcgatggg cgtgctttac catcgccgtt cgccgctgga gcatctctgg
     2341 cagttgaaag atcaactggt gaatgagggt gaactggtgc tggaaacgct ggttattgat
     2401 ggcgatgaga acacggtgct ggtaccaggc gatcgttacg cgcaaatgcg taatgtctat
     2461 ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgtagatatc
     2521 cgcattgtgg atgtgtgcgt taccactact gaagagcagc gacgcactga atggatggtt
     2581 accgaatctc tgtccgattt cctcgaccca catgatccga gtaagacagt tgaaggttat
     2641 cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg tattactgcc
     2701 ggatgcggcg tgaacgcctt atccggccta caaacccttg ctaattcaat ctattgcagg
     2761 gactatgtag gcctgataag catagcgcat caggcagttt tacgtttgca taacctcagc
     2821 gcccgtttcc gggcgctatt cacttcttac gcctgaacag caggcttatc aaatgccgtt
     2881 aacttcggtg cgttacccgt atatttttcg atatacacca gcgcagagtt accggcacag
     2941 gcatttgccc gctgcgagct ggggatatcc gccgttaaca cgttcgcact gccgttttta
     3001 cacaagccgt tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg
     3061 atcccgtcag tcaccaccgc gcctgtcaaa atctgaccac gtttgttcca gacacgcacc
     3121 agatcgccat tcgcaatacc aaatcgagca gcatcttcgg tgtgaatagt gatcggttca
     3181 cgatcggcaa ccgcatattt tttacgcagt tccgcatagt taagctgact gtgtaaacgg
     3241 tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc cttccactca
     3301 tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt
     3361 ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg
     3421 cggaaatcac cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt
     3481 ttattttgct gccagaaggc attaaacatt ggcatagtga cgcgttgcgc acgggcccct
     3541 ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag
     3601 atctcttttc cgccaggttt gagtaattcc gcaagatcgg caaacacgtc aaaatcgtta
     3661 cgcgcttcaa attgcggagc aacagcctgc ttcatcggca caatatgctg gttgctgtaa
     3721 tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct
     3781 gcgtgtttgg ctgctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc
     3841 caggctttga tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac
     3901 cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca
     3961 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgtcattcc gccatcatca
     4021 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca
     4081 cgcgtcgggt tgccaccatt ggagtaatga taagagaaac caaaaccgcc gcccggtgta
     4141 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
     4201 tgctggcgct gaattcccca gcctgccatc agcattgtgc gattcgccgc catcagttca
     4261 gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgtcca gaccgcgctc
     4321 ttcggcgtat tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtagtg
     4381 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
     4441 aacatcaatg gcaatcactg gtttgccaga ttttttcagc tgatggaagt attccagccc
     4501 ttgctcatcg gtactgctcc aggcaatttt taaggtattc agcgggttca ttccccacag
     4561 caccacaacc tggctgtttt ccagaatcag cggccaggag gcctgctgtt catacacctc
     4621 tacagaaccg accacatgcg gcatgatcac ctgtgctgcc ccggttgaat aatcgccgct
     4681 atgcccggaa taaccgcccg ccaggttcat ataacgttgc agtaaggttt gcgctttatg
     4741 caacacgcca gaagagcgcc agccgtaaga tccggcaaaa atggccgatg gtccgttagc
     4801 tttacgaata cgctcatgtt gctcatgaat cagttttaat gcctgttccc agctcacctg
     4861 tacataggta tcttcgccac gacctttcgc cggttgcagt ggattgtcga gatagctttt
     4921 tctcaccatc ggatgctgaa tacgcgccgt ggtgtgtacc tgatccgccg ccgtagactg
     4981 taaggagttc ggtatggttt tcgccagcgc gcctgtcgaa gaaacaatct tgccgtcctt
     5041 cacttctacg ctcatcgctc cccaacgtcc cgcggtgagg attttaccgc ccttctcttc
     5101 tgcccatgcg gggagcggtg ctgccgatgt caccaccagc gctccagcgg caataccgct
     5161 gtgtttaata aattcacgtc ttgttaatgc cataacttcc tccctgatca acgaggatca
     5221 ctgtttctcg gtaatatctt tggcgttgta ctggaaatac cgcgttaaaa tgtccggttc
     5281 gttttcgctc atgctggttc gtgcccccat tcctttggca atggacggcc acgcattgac
     5341 ggtgtaatgg tcggcggcaa tagaggcatg acaaccagcg caataggtat cgtcaagttt
     5401 ttcagcgtat tgccatagcg gtttacggtc cgctaatgcg ggatcggtaa gcgcaccctg
     5461 taaagacgcc tgacgccatt gattgccgta ttcgtcagcc tgccattccc cgtttaccgt
     5521 gagcgccttg ataccttctt cacttaatgt ggctagcgcc agccgttgac ctgccgccag
     5581 gtagagcgtg ttttcactgc cctgcatttg ataaccctgc aacagaacga tcggctgttt
     5641 gccactggca tcaacgacgg tgagatcggt accaggattc acggtagcca gctcgcctat
     5701 gcgagaagtt ttgaaaggat aaatatgtgc gccattagtc actgaagtag cggcctgact
     5761 ttccagctca tgcgccgcgt tgtcatccat ttttatttct ggcggaaaat gggcaatgcc
     5821 tttatgacaa tcgatacagg tttcgccgcc cttttgtgct ttgttatgca ttttctgcgc
     5881 agattcactt tgcgaggcaa tatccatggc atcaaaagaa tggcaactac ggcacgttgc
     5941 agagtcagtt gcttttaatt ctttccatac tgtttcggcc atttcctggc gatgagtttc
     6001 gaacttatcg tcactgtcta ttttgccgct aacaaattca tgataaatat ctttagatgc
     6061 ttttaattta gcaaataaat aatccatccc tgactttgga atatggcaat cggcacattc
     6121 cgcacgtatc cctttctggt tcgaaaagtg gacagttccc tgatattcct caaaaggttt
     6181 actcatcgag tggcaggaaa ggcaaaatgc tgtatccgac gttttatgta aggctttttg
     6241 cgccagcaat aacccgccgc caccaaccac aaccgctatc agcaaaaaca ataacccaat
     6301 gcgttttttc cctcgcatag attcctctaa ataaattcca gaacaccgaa atcattgtat
     6361 aattaaatac acaacgaatt aaccgcttgc ataactcggc acaacactgc ctgaaataat
     6421 cgataaagaa tatgatttat tacaatgtaa tcattaattg ctaaggaata acccagttgc
     6481 tatttagaat atatatttat cagttttagc aatttaaatc ccataatcaa tgtgaatata
     6541 aaaaatattt ttagcacaac gctatgcgca ctgtcacccg ctcaacagat cacggttatc
     6601 gttcgttttt tatactgctc agggataaaa aaaggcccct gttgaaattg caggggcctg
     6661 gtacgagcaa gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg
     6721 gcgttcaatg attcctttca tttcagcaac cgccgccccg tctacgatat aacgcgaata
     6781 ctcgtccgcg tgttcatctg atgacatgga caatccttga ttacgataac gcatcggtga
     6841 ggcttgccac gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg
     6901 caggttttct gcacggactc ctgctccagc cataatgatt ggagcatcac gatgggcaat
     6961 aagttccata atttttgata aaccttgcag tgcgtctgat ttttgccctg atgtcagtac
     7021 ccgggcaatg cccaattccg caagattatt gagtgtatat aaagggttag cgcacatatc
     7081 gaaggcgcga tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg
     7141 tggcatatcg acattcccgt caacatcgag aacgcccgtc accagtccag gaaaacctaa
     7201 ttcgcggacc gtgcgcacat cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa
     7261 atcaccaccg cgtgggcgaa ttatcggatg cacagggatc gtcacccgct ggcgcacgga
     7321 tttcagtacc cccaacgacg gcgttaagcc cccctctttt ggggctgcgc ataattcaac
     7381 tctgtctgcg ccgttttgct gcgccgttag tgcacattcc atgctgtaac agcaaatttc
     7441 cagtaatgcc atttttgctc cttaattacg ctgactgctc gctggcaacg atctcttcaa
     7501 tggaccacgg atgaaactta atagttgttt tgccatcggt gaccgccagc gtcggattcg
     7561 gtaatcgctc atgctcacct tttggtgaac tggttttaac tgagatcccc ggtaggctga
     7621 gtccttcatc agaaagcaat gccagcgcac gggcattcag cgtttctgga tcacccggca
     7681 gaacgatttc aatatgttcc caaccttcgt gtgggtaacg tttttccccg ggccacggta
     7741 gctccacatt ggaaaactgc cagtgcgcaa cctgtaccgg ttcatgcaat ttaaacagac
     7801 aaatcggtct gccattgatc attttttctg acaaaagctc gccacactgt tcaaaaccgc
     7861 gacgccagcg ctcagcggtg acgttttgat ggcaacgcaa agaaatgtga tcggcagtca
     7921 gcggagtgat atccaacccc agacggcggg aaagttcatc taatgcgtgg gtaaatcgcg
     7981 gtaaatccga tgcaatatcc tgcagctcgt cgatagattg ccagttcgcc ataatcactc
     8041 ttcgtctttc agtaaaagcg ttaatttacc ctgttgccct gtgccaacca accgctgatt
     8101 tcacgccgct tctgatgcaa tagtgaaaac ggcaatacgc cacgcgcacg ttgctgacga
     8161 aaacagccat ttgcagtata ctcccgccct aatttcttta actggtgcgg gcaatttttg
     8221 ctcgcttcat caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc
     8281 cgtcaggcca tgattgcggc aggcgcgcct gcggattgcg aaccgcagat tcgtcagtca
     8341 gcaaaagttc agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg
     8401 ggtatggcac cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc
     8461 gccagcaaag ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc
     8521 ctggctgaac atgttcagca ggcgctggcg tccgatcgtc tcggtgttgc tacgccagaa
     8581 aaacagacca ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcacgtcggt
     8641 cacctgcgct ctaccattat tggtgacaca gcagtgcgta ctctggagtt cctcggtcac
     8701 aaagtgattc gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca
     8761 tggctggaaa agcagcagca ggaaaacgcc ggtgaaatgg agctggctga ccttgaaggt
     8821 ttctaccgcg atgcgaaaaa gcattacgac gaagatgaag agtttgccga gcgtgcacgt
     8881 aactacgtgg taaaactgca aagcggtgac gaatatttcc gcgagatgtg gcgcaaactg
     8941 gtcgacatca ccatgacgca gaaccagatc acttacgatc gtctcaacgt gacgctgacc
     9001 cgtgatgacg tgatgggcga aagcctctac aacccaatgc tgccaggaat tgtggcggat
     9061 ctcaaagcca aaggtctggc agtagaaagc gaaggggcga ccgtcgtatt ccttgatgtg
     9121 tttaaaaaca aggaaggcga accgatgggc gtgatcattc agaagaaaga tggcggctat
     9181 ctctacacca ccactgatat cgcctgtgcg aaatatcgtt atgaaacact gcatgccgat
     9241 cgcgtgctgt attacatcga ctcccgtcag catcaacacc tgatgcaggc atgggcgatc
     9301 gtccgtaaag caggctatgt accggaatcc gtaccgctgg aacaccacat gttcggcatg
     9361 atgctgggta aagacggcaa accgttcaaa acccgcgcgg gtggtaccgt gaaactggca
     9421 gacctgctgg atgaagccct ggagcgtgca cgtcgtctgg tggcagaaaa gaacccggat
     9481 atgccagctg acgagctgga aaaactggct aacgcggttg gtattggtgc ggtgaaatat
     9541 gcggatctct ccaaaaaccg caccacggac tacatcttcg actgggacaa catgctggcg
     9601 tttgagggta ataccgcgcc atacatgcag tatgcttaca cgcgtgtatt gtccgtgttc
     9661 cgtaaagcag aaattaacga agagcaactg gctgcagctc cggtaatcat ccgtgaagat
     9721 cgtgaagcgc aactggcagc tcgcctgctg cagtttgaag aaaccctcac cgtggttgcc
     9781 cgtgaaggca cgccgcatgt aatgtgtgct tacctgtacg atctggccgg tctgttctct
     9841 ggcttctacg agcactgccc gatcctcagc gcagaaaacg aagaagtgcg taacagccgt
     9901 ctgaaactgg cacaactgac agcgaagacg ctgaagctgg gtctggatac gctgggtatt
     9961 gagactgtag agcg
//
LOCUS       DAJTYM010000125         9896 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTYM010000125 DAJTYM010000000
VERSION     DAJTYM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(150..890)
                     /gene="pflA"
                     /locus_tag="OO575_003432"
     CDS             complement(150..890)
                     /gene="pflA"
                     /locus_tag="OO575_003432"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6666268.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OO575_003433"
     CDS             complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OO575_003433"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6666269.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OO575_003434"
     CDS             complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OO575_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6666270.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4682..6442)
                     /locus_tag="OO575_003435"
     CDS             complement(4682..6442)
                     /locus_tag="OO575_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6666271.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6572..7198
                     /locus_tag="OO575_003436"
                     /pseudo
     CDS             6572..7198
                     /locus_tag="OO575_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7397..8485
                     /gene="serC"
                     /locus_tag="OO575_003437"
     CDS             7397..8485
                     /gene="serC"
                     /locus_tag="OO575_003437"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6666272.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8556..9839
                     /gene="aroA"
                     /locus_tag="OO575_003438"
     CDS             8556..9839
                     /gene="aroA"
                     /locus_tag="OO575_003438"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6666273.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2519 a         2367 c         2478 g         2532 t
ORIGIN      
        1 aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat atggtagatg
       61 cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa aaagcggcaa
      121 ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc gtactgctca
      181 agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc atcgagtttg
      241 tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg gagaagttcg
      301 attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc cgaatcgtca
      361 tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt tttgttcgcc
      421 agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt ttggtggatc
      481 tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag cagttcatca
      541 atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg aatgccttct
      601 tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc ttcaccgccg
      661 gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac cacttccttc
      721 atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc gcggttatga
      781 caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat acctgggccg
      841 tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat tgcggtgttt
      901 ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct
      961 gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt gctaaaaaag
     1021 gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta
     1081 attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg
     1141 agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat ttttccgggt
     1201 tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg tgctgaccac
     1261 cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg ttggtcttac
     1321 gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag gagataccat
     1381 ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca gcacctttct
     1441 ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt
     1501 ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc agaacagact
     1561 gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg aaacgttcta
     1621 ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc gggtattcgc
     1681 cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta actttcgcat
     1741 atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca cacgccatgg
     1801 tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt
     1861 gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag tccatgaagt
     1921 gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc ggttcagact
     1981 tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg tacagcatgg
     2041 ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg atcatcgggc
     2101 ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt
     2161 tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc ttgaagttca
     2221 gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc cccatagtgt
     2281 acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca tcgaggccca
     2341 taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca tcgtattccg
     2401 gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc atttcctgcg
     2461 cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca tccaggaagg
     2521 tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca gccaggtagc
     2581 cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag atgtcgtagc
     2641 cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca gcgatttctt
     2701 cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc tgtagagagg
     2761 tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca acgcgacggt
     2821 agtcaccgat gatacggcca cggccataag catctggcag accggtcaga acaccggatt
     2881 tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg tgagttttac
     2941 ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac gctttgcagg
     3001 aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc ggagcttcag
     3061 tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg tgagaggtga
     3121 tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt tccagtttaa
     3181 caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca gccaggaagg
     3241 actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg tttacttcat
     3301 tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca ttaagctcgg
     3361 acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc gacaaccatt
     3421 aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc aacaaaccac
     3481 cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag ttcatcacgg
     3541 tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc ggagatgcga
     3601 agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg tgctcaaaac
     3661 cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc atcaggctgc
     3721 ggccagaata actcatccat actgccagac ataccatcag gtttgccagg ataccaagac
     3781 agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg acgtttagtc
     3841 cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt acaaacagca
     3901 gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac tgacaccagg
     3961 tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag agatcggctc
     4021 cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc agttttgcca
     4081 tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg aatgcgattg
     4141 agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt ttcgttgctt
     4201 tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa agatcaaaag
     4261 ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat actaacaaag
     4321 cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc ccgtaactcg
     4381 catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa taaattttaa
     4441 aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta attaaaaggg
     4501 agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa ttaccgatat
     4561 ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg ggcattaaaa
     4621 aaacgccccg taatataact cagactaatc attaagccta cattgcgtag gctatttgat
     4681 tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacaacg
     4741 actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg taaaacgccg
     4801 cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac atgcgaacaa
     4861 acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat aacaacgttt
     4921 gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta aactccatcg
     4981 tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt
     5041 ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc agacccaaca
     5101 gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg atgaggttca
     5161 ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca cgtaaatggc
     5221 tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg gacatgccag
     5281 gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata taaacttctt
     5341 tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg gtgccggaga
     5401 aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac
     5461 cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct tcatcatcga
     5521 aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc agctcggtca
     5581 cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc
     5641 cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca agggaaccat
     5701 catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca accaccgctg
     5761 ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca gcaataatgc
     5821 ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttgcggg
     5881 tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc atcggaatat
     5941 aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca cgatcttcgt
     6001 taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga tcgtaaaatg
     6061 cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc agtgggaacc
     6121 atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc agccagaaat
     6181 cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca gaagccagcg
     6241 ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa
     6301 tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca atctgaaagc
     6361 cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg gcatctttgc
     6421 cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata
     6481 cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt gactatgctt
     6541 ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc accgtatggc
     6601 atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc tttatacctt
     6661 tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca gatgtcgctg
     6721 tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc gttttatgat
     6781 gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt ataccgcctg
     6841 gtaatgtgat ggcgagctgg cctggtcgaa actcaataac tccaacatga cggaatttga
     6901 gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac gtctggcgat
     6961 tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg tgaaaccggg
     7021 tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg agtcggcgga
     7081 ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa aacaattatg
     7141 tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg tgacctgaca
     7201 gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat gcaagccaca
     7261 tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata aaaccgatag
     7321 ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta atagcaacgc
     7381 aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg caatgctacc
     7441 ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc ttggtacgtc
     7501 ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg aagccgagaa
     7561 ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct gccatggcgg
     7621 tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa ccaccgcaga
     7681 ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa aatactgcac
     7741 gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta agccaatgcg
     7801 tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg aaaccatcga
     7861 cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg ccgccgactt
     7921 ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa tttacgctgg
     7981 cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg aagatttgct
     8041 gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca acgataacgg
     8101 ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg tctttaaatg
     8161 gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa aagcagaact
     8221 gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga aagctaaccg
     8281 ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat tgttccttga
     8341 agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg gcggaatgcg
     8401 cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag acttcatggt
     8461 tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac agccagcctg
     8521 tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg ttacaaccca
     8581 tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct aaccgcgctt
     8641 tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg gatagcgacg
     8701 acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg ctttcagccg
     8761 atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa ggtgccctgg
     8821 agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct ctttgtctgg
     8881 atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg attggtcatc
     8941 tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa gaaaattatc
     9001 cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat ggctccgttt
     9061 ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa gatacggtga
     9121 ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc aatctgatga
     9181 agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta aaaggcgggc
     9241 agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg gcttcttact
     9301 tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt ggacgtaaca
     9361 gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg accatttgct
     9421 ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat atggatatga
     9481 accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca aaaggcacca
     9541 ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg tttgcgatgg
     9601 caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac attcgtatca
     9661 ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac cggatggcga
     9721 tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat cccaaatgca
     9781 cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag gcagcctgaa
     9841 tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca cattgt
//
LOCUS       DAJTYM010000126         9301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTYM010000126 DAJTYM010000000
VERSION     DAJTYM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1013
                     /locus_tag="OO575_003439"
     CDS             <1..1013
                     /locus_tag="OO575_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666274.1"
                     /translation="DVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAA
                     RKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELP
                     QSVEYVPLACEAHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIE
                     MIKNRMAELFNQSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINET
                     LEGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDS
                     LTPEELMMLKDLFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGM
                     GV"
     gene            1692..1910
                     /locus_tag="OO575_003440"
     CDS             1692..1910
                     /locus_tag="OO575_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6666275.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2128..2370
                     /locus_tag="OO575_003441"
     CDS             2128..2370
                     /locus_tag="OO575_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666276.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2700..3629)
                     /gene="fdhE"
                     /locus_tag="OO575_003442"
     CDS             complement(2700..3629)
                     /gene="fdhE"
                     /locus_tag="OO575_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6666277.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3626..4261)
                     /gene="fdoI"
                     /locus_tag="OO575_003443"
     CDS             complement(3626..4261)
                     /gene="fdoI"
                     /locus_tag="OO575_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6666278.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4258..5160)
                     /gene="fdoH"
                     /locus_tag="OO575_003444"
     CDS             complement(4258..5160)
                     /gene="fdoH"
                     /locus_tag="OO575_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6666279.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5173..8223)
                     /gene="fdnG"
                     /locus_tag="OO575_003445"
     CDS             complement(5173..8223)
                     /gene="fdnG"
                     /locus_tag="OO575_003445"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7636..7638),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6666280.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8417..9250
                     /gene="fdhD"
                     /locus_tag="OO575_003446"
     CDS             8417..9250
                     /gene="fdhD"
                     /locus_tag="OO575_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6666281.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2263 a         2288 c         2419 g         2331 t
ORIGIN      
        1 aagatgtagg tccagatata cttgcattgc ccgtcaacgt atatcatgcg gattttaaaa
       61 ttaaaatcaa taatgcagtg gagaaggctt catcaattgt gttatgtgct gtgcaaaaaa
      121 atctccatgc agcaaggaag gttcttagcg ggataatagg aaatgtgcca aaaaataata
      181 aaccaaaaac aattattttt gatttgcgtt ttcttagtgc aattaagcag aaagtttttt
      241 tgggcagtga aaaaccgttg tttgtaaagc ataccgcaat gatgagggag gcatgtaagg
      301 aattaccgca gtcagtggaa tatgttcctt tagcctgtga agcgcatagc catcgcagcg
      361 tcgcccttgc caaaaccgtg gctgcgccca tcatggcgac aggtgtgggc ttggcattaa
      421 tgtatgcaac atcatcagga ccgtattatg cgcaaagtct ttctggttcg ccggatatcg
      481 agatgataaa aaatcgcatg gctgaattgt ttaaccagtc ggatatttct gtgcgcaaca
      541 aatatagccc ggcttttgat aaatggaatg aactggtaga taaattaaat cacgaaagaa
      601 atacagttcc gtttgcgtgt ttgaccacca ttttagcgac ggcgataaat gaaactcttg
      661 agggggatac gaatgttgcc gttgtgatgg ggtgtaaatc tgcgaaagat cgcacgatat
      721 ccattgtcct tggcaactcg atgttgcaaa cactccttga aaaacgcctc gccgatagtg
      781 gagaaataga aaatcttttt gatcaacagg gatattttag ttgcgattca ttaacacccg
      841 aagaattaat gatgttaaaa gacctttttg atattcgagt tcttcatgtc tctaacaaat
      901 tcaatgttgg attgcaaggt aatatcaaca ctgatgtttt acaagattcg ttttttaaaa
      961 atgtcgattt cattcatgaa tataactcgt ataccgtagg gatgggagtc tgacgagtaa
     1021 aactcattag gattatcaga attcggacaa tgcattgcaa aaatgacata caaagagaga
     1081 tgtgtgcagg tacttagccg cgaggggcat ggcgaagcca tacagaaagt accagtattc
     1141 agaactccag tgaaagcggc atagtggccg cctgctcagg agttttgcta tgacccgtaa
     1201 tcgtgctgaa cgtcgccacc ctatgatgcg attaaaaaaa ctcatcgtcg cgctgatgat
     1261 tgacgtcaat gctatcggat gatgccgggg gtgttccgga aggaattcct tgtttcccga
     1321 actgttacca acaaaattat aacgatgagc aagtaaagac cggttggtgg cagtactgac
     1381 cggtctgtga tgaaagatta atttcacctt aaatcctggt gctgttagga tgctgacgtt
     1441 tttcggctac accattcaga ttatgtaaat aaatatccct gaatataata tattcatagc
     1501 acccataact catcctgggc gataactaaa ccatttttga gtaataaatg aaacaactgc
     1561 aacgctctta ttatttctgg aaaacgcctc gcaaaaacaa aatgacatgc aaaaatccgc
     1621 atttttttaa atgcttttcc ggcgtaattt tcgcacatac attaatgagt aatatatgta
     1681 aggtggtggt aatggcgatg aatacggttt ttcttcattt atcagaagag gcaattaaac
     1741 ggctgaacaa gcttcggggc tggcgtaagg tttcacgatc tgcaatttta cgcgaagcgg
     1801 ttgagcaata tctggagcgg cagcaatttc cggtgcgtaa agcaaaggga ggcaggcaaa
     1861 agggggaggt agttggtgtt gatgatcagt gtaaggagca taaggaatga aaaaaatttt
     1921 tcattcttga atataaaaaa cagatgccct tattctggta ttaatacaag gctgttttac
     1981 ttgaacttat aataactgca actgttacat cgtatctgga aaacgcctcg caaaaataaa
     2041 aaatgatgcg taaatgagct ttctttaaag attcggcaag ggtacttttc tctccataga
     2101 ttcaaaaatg atattggcga gatatttatg aactctctgg caggtataga tatgggcaga
     2161 attttactcg atttatcgaa tgaggtgatt aagcaacttg atgatctcga ggtgcagcgt
     2221 aatcttcctc gtgcggacct attaagggaa gcggtagatc aatacctgat aaatcaatcg
     2281 caaacagcaa gaaccagtgt tcctggcatc tggcaagggt gtgaggaaga tggtgtcgaa
     2341 tatcagcgta agctgcgcga ggaatggtaa tgggaaaaaa tgatgtcaat cagattgctg
     2401 acaacgtgcg cgttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaagtc
     2461 atgcaaattc aatatattga agtttattgg taggcctgat aagcgcagcg catcaggcaa
     2521 tcatacgctt gtcatcagtg acatacccat aaccttatcg atgtgttgga gggaagaacg
     2581 aggcaaacag acaattgcgg catgatcttg aatgggtaac gcggaatacg agagatggtg
     2641 tcgtcacacc ctatgagctg taatccgggc gacctgcgcc acccggaaaa cgtcagagat
     2701 tactctcctt cacccggaaa caggaacggg ttaatggaac tacgggcgta gccttcttgc
     2761 tccattcgcg cgtccagtac cagagaggcg aggtcatctg ccacggcttc aactttcggt
     2821 tctttttcct gataaagaat cttcaggtaa gtgccgcagt catcgcagct ttcggcttta
     2881 atcgcggcct gttcgtcatc cagcgaccag taatgcagtt tgccgctctg ctcgcagttg
     2941 ctgcatttta cgcgcactac gtgccattcg gtttcacaca ggttgcagtg caggtaacgc
     3001 agaccctgag tggtgccaat ttgcaccatg ctggacaccg gcatagagcc acataccggg
     3061 caatattgac gttgttcgcc gtattcagcg cgggctttgc cggggatcag attggccatc
     3121 tgcgcccagt agagcgacag cgcagcccag ataaacggcg ctttatcgct gctgacggac
     3181 gagaaatcag aggcaaacag cgcgctggcc atatcttcca gctcctgagt cgatgccttc
     3241 tccagattct caatcactgc cagcgccggg ccgctcattt caggtttcag ctcagcaatt
     3301 agcgccatca gcagcttttg ccagtgctta tcacgcggca gaacgtgaat atccagcggg
     3361 ggcttgccct gtgcgctggc ttctttaatg cgcgcggtca gatccatctc cagcggatgg
     3421 tcgtacagca ccactttctg ggcgtgggcg ataagcgcag caaagcgcag gtaatcaccc
     3481 agcggattat tttctgccag ctcgcgcaga cgctcggcgc ggcggttgta taaattcttg
     3541 agccgaggga acaataacgg cggaatcata tccgccgtac gtttctcgct cgaacccagc
     3601 tcatcttgcg ggattatgcg aatactcatt cagctttctt ttccgttgtc ttgcggacct
     3661 cacggtacca gcgcgggtga tgtttcttcg cccaggcgct ggtaacccat ccttccacca
     3721 tggcggtaat cgtgcctttc acccaaaggg cggcgtagat atgcaccatg ataaccacaa
     3781 ttaacgccac tgcggcaaat gaatgcagca ttaacgcgaa tcggatcacc gggattgaga
     3841 aagcaggcgc aaaataagga cgccagataa tcacgccgct caccagcagc agaaccagga
     3901 aaataatcgc cgcccagaaa acgcatttct gaccgaagtt ataacgcccg gtgtcaccta
     3961 cttcctcgtt gacgacgatc ttacgaatat tcttcgccca aaagatatca tcccgattga
     4021 ttaggttttg atgccagtaa cggaaaaaca tgatgatgaa cgaggcaaac ataaccacgc
     4081 cgacaaacgg gtgcagaatt cgcgccagct gcggtgtgcc catgatttgc atcaaccagt
     4141 tgaaggacgg gaacaaaaag cccagcccgc tcaccgccgc caggatgaag cagaaggcgg
     4201 tgatccagtg gttgatacgt tccggcgcgg tgtagcgcac gatggtgtca cgtcgtttca
     4261 tttgcgctcc tcgtctttct cttcgtgcag attattctct tcctcatccg cacggttcgg
     4321 accgacaccc acgtagtgga agatactggc cgcaaaggta gccgcaaagc caacagccgc
     4381 gagcggtttc cagatgcctt tccagaattt cacggtttcg ctgatttccg ggttctccgg
     4441 caagccatga tacagatttg gcttgtcagc atggtgcagc acgtacatga cgtgtgtacc
     4501 accgacgcct gccggatcgt acagacccgc attgtcgtaa ccgcgggttt tcagctcagc
     4561 aacgcgctcg ctcgccagcg ttttcatcga ctctttcgtg ccgaagtgaa tcgcgcccgt
     4621 tgggcaggtc ttcacgcagg ctggttcttg cccaaccacc acgcggtcaa cgcacagcgt
     4681 acatttgtag acgcggttgt cttccgggtt gaggcgcgga atgtcgaacg gacagcccgc
     4741 aatgcaataa ccgcagccaa tgcactgctc ggactggaag tcgacgatac cgttggcata
     4801 ctgaataatt gcaccttccg ccgggcacgc tttcaggcag cctggatcgg aacagtgcat
     4861 acagccgtct ttgcggatca gccattccag tttgtcgttc tgctccactt ccgagaagcg
     4921 cattaccgtc cacgatttgg cgcttaaatc attggggttg tcgtacaccc caatgttatt
     4981 gccgacggta tcgcgaatgt cgttccactc tgaacacgcc acctgacagg ctttacagcc
     5041 gatacaggtg gtaacgtcga tgagtttcgc cacttcttcc tggaagtccc gcgcctgagg
     5101 cgcgggggtc agaccgttag tcgcggaacg acgaatgata tcttgagatt gataagccat
     5161 atgtcgtctc cgttacacct tttccacatt cacaaggaag gacttaaact ccggcgtctg
     5221 cgtgttcgca tcaccaacga atggcgttaa agtattggca ataaagcctt ttttagctac
     5281 gccttcatag ccccagtgaa tcggaatacc gatggtatcg atatctttgc cgttcgcttt
     5341 cagcgtgcga atacgtttgg tcaccaccgc tttggctttg atatagccac ggttggagga
     5401 gactttcacg gtatcgccct gggcaatgcc aagtttattc gccagcgact ccccgatttc
     5461 cacaaactgc tctggctgca aaatcgcgtt caacagcgcg tgtttggtcc agtagtggaa
     5521 gtgctcggtc agacgatagg tggttccgac atacgggaac ttatcggctt tacccaatgc
     5581 ttcggcgtcg tctttaaaga tacgtgcagc tgggttcgag ataacgtttg gatgcagcgg
     5641 gttagttccc agcggcgttt caaacggctc gtagtgttcc gggaacggac cttccgccat
     5701 cttatcgagg gcaaacagac gccccatgcc ttcctgctgc atgataaacg gcccgacgcc
     5761 gctacccgga ggcgctgcgc tgtagtccgg aatatcccag ccggtccact tagtgccgtc
     5821 ccatttcagc aactgacgct tcggatccca cgggttaccc tgcggatctg cggaggcgcg
     5881 gttatacaga atgcggcggt taagcggcca tgcccatgcc cagcccagcg tgttaccgag
     5941 gccagacgga tcggcgttat cacgacgcgc catctggttg ccttccggcg tccagctacc
     6001 ggcgaaaatc cagcagccac aggaggttgt accgtcatcg cgcaattggg cgaacgagct
     6061 aagttgttgg cctttcttga cgataaccgc accggttgcc ggatcggtaa tatcggccag
     6121 cgccttaccg ttgctctcca tcgctacttc ttccgacttc ggctcatgcg gaatggcgta
     6181 gttccaggtc atgttcagca cctgatccgg gttcgcgcca ccctgttcgg catacatctt
     6241 gcgcaagcgc aggaagatac cggagaggat ctcaccgtca gtcagcgcaa tccccggggc
     6301 gtccgcacct ttccagtgcc actgcaacca gcgacctgag ttaacgattg aaccgttctc
     6361 ttctgcgaag caggtcgatg gcagacggaa cacttcggtc tggatcttcg acgagtcaac
     6421 ttcgttcagc tcaccgtggt tctgccagaa gttagaggtt tcagtgttca gtgggtcgat
     6481 ggtgacgagg aacttcagtt tcgacagaca accgatcact ttgtttttgt tcgggaatga
     6541 ggcaacaggg ttaaagccct ggcagatata gccattgacc ttgccctctt tcatcatctc
     6601 gaagtattgc aggacgtcgt agcctttatc ccacttcggc aaccagtcaa agccccagct
     6661 attttccgcc gtcgctttat caccaaagaa ggccttcatc atagagacga agaatttcgg
     6721 gtagttgccc cagtagttta cctggccttc cagcagcggt tttggcgtgt tggcggtaag
     6781 gtaggtttgc agatcggtct gcttctcgct tggcagcgtc atgtaacctg gcagactctg
     6841 cgacagtagc cccaggtccg tcagcccctg aatattggag tgaccgcgca gggcgttaac
     6901 gccgccgccg gccatcccca tattgccgag cagcagctgg atcatcgcca tcgtacgaat
     6961 gttttgcgca ccaacggagt gttgcgtcca gccgagggca tacaggaacg aggcagtttt
     7021 atcgtgagca ctggtttctg cgatgtattc gcagactttc aggaacgcgt cttttggcgt
     7081 accacagatg ttttcaacca catctggcgt gtagcgggaa acgtgctgtt tcagcaagtt
     7141 ccacacgcag cgcggatgtt gcagcgtggt atcgcgtttg gcgaagccgt tttcgtccag
     7201 ttcataagtc caggaggatt tatcgtactt gcgtttttcc gcgtcgtagc cggtgaacag
     7261 gccatcttca aagccgtaat cctcacgcac gatcaggctg gcgttggtat aggcttcggt
     7321 gtattcgtgg ttgaattttt cattgttcag caggtacagc aatacgcctg acaggaaagc
     7381 aatgtcagta ccggaacgaa taggggcata gtagtcagct accgccgccg tacgcgtaaa
     7441 gcgaggatcg atcacaatca gcttcgcgcc gttgtgaatt ttggcttcca tcgcccagcg
     7501 gaacccgacc gggtgagctt cagcggcgtt accgcccatc accacgacga ggttggcgtt
     7561 cttgatgtcg acccagtggt tggtcatcgc accgcgacca aatgttggag caagacttgc
     7621 taccgttggt ccgtgtcaga cacgcgcctg gttgtcgacc gcgagcatac ccagcgcgcg
     7681 ggagaatttt tgcgttaaat agccggtttc gttactcgac gcggaagcac acagcatccc
     7741 ggtggagagc cagcggttaa cagtcacgcc ttcggcgttt tgcgcaatgt agttagcatc
     7801 gcggtcttct ttcatcagtt tggcgatgcg atcaaacgcc tcttcccaac tgatttgctg
     7861 ccatttatca gaacctggcg cacggtattc cggaaacttc agacggcttt cggagtggat
     7921 gaaatccacc aggccagcgc ctttcggaca aagtgcaccg cggttgaccg ggtgatccgg
     7981 atcgccttcg atatggaaga tagatgcttt ggcgtttttt gctccgtcac cgaggctgta
     8041 catcaacagc ccacagccta cggaacaata ggtgcaggta ttacgggttt cgcgggtgcg
     8101 cagcagttta tactgccggg tttccgcgag tgctacgctg ggtgcaaaac ccagtgccgc
     8161 tgccgtggtg cctgccatac cgccagcgca gatcttaaag aactgccttc tgctgacctg
     8221 catggattgc tccttgtttc gacattgtca cgtcccattt acattcgctt gctgcgtgtg
     8281 cagggagtgg gagttatttt tctttgcgga aggggccgca aaggtccaga attggctcaa
     8341 tttccctcca tctaggaagg gtttgtaaca gaataccata atgttggtgt gtatgttctt
     8401 atctggttaa gagaaagtga aaaaaacaca gcgaaaagaa atcgaaaatg tgacaaatat
     8461 cacaggtgtt cgtcaaattg agttatggcg gcgtgatgat ttacaacacc cacggttaga
     8521 tgaggtcgcg gaagaagttc ccgttgcgct ggtctacaac ggcatttcgc atgtggtgat
     8581 gatggcgtcg cccaaagacc ttgagtactt tgcgctcggt ttttcgcttt ccgaggggat
     8641 aatagaaagt ccgcgcgata tcttcggcat ggatgtcgtt ccttcctgta atggtcttga
     8701 agtacaaatt gagctttcca gccgccgctt tatggggttg aaggagcgcc gccgggcgct
     8761 ggcgggacgt acgggctgtg gcgtatgcgg cgtggagcaa cttaatgaca tcggaaaacc
     8821 ggtgcagccg ctaccgttca cccagacgtt tgatctcaac aaactggatg atgcattacg
     8881 tcatctcaac gattttcagc cagtggggcg actgactggt tgtactcacg ccgctgcctg
     8941 gatgttgcca tctggcgaac tggtcggcgg gcatgaagac gtgggtcgcc atgtggcgct
     9001 ggacaaactg ttaggccgcc ggtcacaaga aggggaaagc tggcagcaag gtgcggtact
     9061 ggtttccagt cgtgccagtt atgaaatggt gcaaaagtcg gcgatgtgcg gcgtagagat
     9121 tttgtttgcg gtgtctgccg cgaccacgct tgctgtagaa gtggctgagc gctgtaatct
     9181 gacactggta ggtttttgta aaccgggtcg ggcaacggtt tatacccatc cgcagcgttt
     9241 gagcaattaa ttaaaataag tcctgcgata tatattaaat tgcaggactt attcatttcg
     9301 t
//
LOCUS       DAJTYM010000127         9281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTYM010000127 DAJTYM010000000
VERSION     DAJTYM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..246
                     /gene="mliC"
                     /locus_tag="OO575_003447"
     CDS             <1..246
                     /gene="mliC"
                     /locus_tag="OO575_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6666282.1"
                     /translation="QTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLSASG
                     ARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            305..961
                     /gene="pdxH"
                     /locus_tag="OO575_003448"
     CDS             305..961
                     /gene="pdxH"
                     /locus_tag="OO575_003448"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6666283.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1090..2364
                     /gene="tyrS"
                     /locus_tag="OO575_003449"
     CDS             1090..2364
                     /gene="tyrS"
                     /locus_tag="OO575_003449"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6666284.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2423..3286
                     /gene="pdxY"
                     /locus_tag="OO575_003450"
     CDS             2423..3286
                     /gene="pdxY"
                     /locus_tag="OO575_003450"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6666285.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3330..3935)
                     /gene="gstA"
                     /locus_tag="OO575_003451"
     CDS             complement(3330..3935)
                     /gene="gstA"
                     /locus_tag="OO575_003451"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6666286.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4041..5543)
                     /gene="dtpA"
                     /locus_tag="OO575_003452"
     CDS             complement(4041..5543)
                     /gene="dtpA"
                     /locus_tag="OO575_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6666287.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6154..6789)
                     /gene="nth"
                     /locus_tag="OO575_003453"
     CDS             complement(6154..6789)
                     /gene="nth"
                     /locus_tag="OO575_003453"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6666288.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6789..7484)
                     /gene="rsxE"
                     /locus_tag="OO575_003454"
     CDS             complement(6789..7484)
                     /gene="rsxE"
                     /locus_tag="OO575_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6666289.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7488..8108)
                     /gene="rsxG"
                     /locus_tag="OO575_003455"
     CDS             complement(7488..8108)
                     /gene="rsxG"
                     /locus_tag="OO575_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6666290.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8112..9170)
                     /gene="rsxD"
                     /locus_tag="OO575_003456"
     CDS             complement(8112..9170)
                     /gene="rsxD"
                     /locus_tag="OO575_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6666291.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2381 a         2325 c         2353 g         2222 t
ORIGIN      
        1 caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat
       61 ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa acagggcctt
      121 tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa aggtgaggaa
      181 gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca aaatccacag
      241 cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa
      301 aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa
      361 aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg aacgttggct
      421 ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg ctaccgtgga
      481 tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg aaaaaggcat
      541 ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata atccgcgcgt
      601 tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg gtaaagcaga
      661 acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg atagccagat
      721 tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa
      781 attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga gcttttgggg
      841 cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc atcgcctgca
      901 tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc ttgcaccctg
      961 aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac tttattctat
     1021 gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg
     1081 gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
     1141 caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat cgcgctctat
     1201 tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
     1261 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
     1321 ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga agaaactgtt
     1381 caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt cgactgtgga
     1441 gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa tgtgctgacc
     1501 ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
     1561 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
     1621 ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct gcaaattggt
     1681 ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg tctgcatcag
     1741 aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac caaatttggt
     1801 aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta caaattctac
     1861 cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt cttcaccttt
     1921 atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg taaagcaccg
     1981 cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga agaaggttta
     2041 caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc gctgagtgaa
     2101 gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga aaagggcgca
     2161 gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa
     2221 actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc tgaatacttc
     2281 tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat
     2341 tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc
     2401 ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt ctcacgttgt
     2461 ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg gcgcgaacgt
     2521 ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat ggactggctg
     2581 cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt
     2641 acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg gtgaacatat
     2701 cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt tttgcgatcc
     2761 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
     2821 gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc tggaaatact
     2881 ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattga
     2941 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
     3001 ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc cactggtgga
     3061 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
     3121 actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg cagtctacga
     3181 aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg ctgctcagga
     3241 tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata tctcatttcg
     3301 gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct
     3361 tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc taacccttcc
     3421 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
     3481 gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac atattgcagc
     3541 ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc tgggcgaaac
     3601 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
     3661 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
     3721 tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag caatgcaggc
     3781 acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
     3841 aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
     3901 agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt
     3961 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg
     4021 taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc tgcagcgtca
     4081 tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc aataacggca
     4141 gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat cagcgggtcg
     4201 gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag gtttgcaccg
     4261 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttgt
     4321 gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg caggccatag
     4381 cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt cgcacccagc
     4441 ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt ggttggcatc
     4501 ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc gatgatgatc
     4561 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag aatggagtgc
     4621 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta cagcacgaag
     4681 aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
     4741 ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac aacgcccagc
     4801 gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat cagcgccaca
     4861 acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc tggttttgaa
     4921 ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
     4981 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggggtggct
     5041 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
     5101 agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
     5161 aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc gtcgtgacca
     5221 gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
     5281 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
     5341 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
     5401 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
     5461 gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt
     5521 ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt
     5581 gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
     5641 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
     5701 aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
     5761 aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg cggcgatatg
     5821 ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat agccagtgta
     5881 atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa cctgattctt
     5941 gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata gaaaatttcg
     6001 ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta cctgatgata
     6061 acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat aaagaaaggt
     6121 tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct ctttgtattc
     6181 acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc aggtataacg
     6241 cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa ccactttcag
     6301 tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac gattacaaac
     6361 gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt ttaatacgac
     6421 gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat cttccggaac
     6481 ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt ctgctttgct
     6541 gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta gttcaagcat
     6601 cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa cactgatatc
     6661 ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac tgaaattaag
     6721 ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca gacgttttgc
     6781 tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc agctgcttca
     6841 gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat cagtcccaag
     6901 ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt gtggaaaatc
     6961 tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc aaacaacgtg
     7021 ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt tgcgcccgta
     7081 ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc gaaggcttca
     7141 gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc taatgattga
     7201 tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac cgaggcgatg
     7261 atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag cgtcgaaatg
     7321 gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag agcgttagtg
     7381 gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag tgcagagttg
     7441 tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta ttctccacag
     7501 gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc gcgttttacc
     7561 gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa atcaccacca
     7621 tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc cgcaaaatgg
     7681 gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt ttcgtggtgc
     7741 tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag cagctgaatc
     7801 gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg tttgtcatcc
     7861 tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt taccagatag
     7921 caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc aaataacgtc
     7981 ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt gatggccgca
     8041 gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt tcggatagtt
     8101 ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta gtaatcgatc
     8161 agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg atagccgccg
     8221 aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag ccgaccacga
     8281 ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag catagtcgca
     8341 ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa ccagcccaac
     8401 gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg aatcgctttc
     8461 tgccatagca accacacgcc accagccagc caggcgagat ttacccattg ccaaccagcg
     8521 cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga atgacctgcg
     8581 cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc aatacctaag
     8641 cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac ctgtatggcg
     8701 tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct ggtcatctgc
     8761 accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa cgggttttgc
     8821 cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc cagtacgacc
     8881 atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt cagcaatgct
     8941 gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac gagagcttcg
     9001 gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca accaaaaaac
     9061 cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat aatgcgcgat
     9121 gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat ttagtcctcg
     9181 tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc cgcggcaact
     9241 gcggctttgc gcggatcgac ctgttcttct ggttccgcat t
//
LOCUS       DAJTYM010000128         9167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTYM010000128 DAJTYM010000000
VERSION     DAJTYM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..925
                     /locus_tag="OO575_003457"
     CDS             <1..925
                     /locus_tag="OO575_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6666292.1"
                     /translation="LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAP
                     PALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRP
                     QLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAI
                     PFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAV
                     AFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDT
                     THSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1177..2094
                     /gene="oxyR"
                     /locus_tag="OO575_003458"
     CDS             1177..2094
                     /gene="oxyR"
                     /locus_tag="OO575_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6666293.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2077..3477)
                     /gene="sthA"
                     /locus_tag="OO575_003459"
     CDS             complement(2077..3477)
                     /gene="sthA"
                     /locus_tag="OO575_003459"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6666294.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3798..4448
                     /gene="fabR"
                     /locus_tag="OO575_003460"
     CDS             3798..4448
                     /gene="fabR"
                     /locus_tag="OO575_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6666295.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4448..4807
                     /locus_tag="OO575_003461"
     CDS             4448..4807
                     /locus_tag="OO575_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6666296.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4847..5947)
                     /gene="trmA"
                     /locus_tag="OO575_003462"
     CDS             complement(4847..5947)
                     /gene="trmA"
                     /locus_tag="OO575_003462"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6666297.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6061..6251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6316..8160
                     /gene="btuB"
                     /locus_tag="OO575_003463"
     CDS             6316..8160
                     /gene="btuB"
                     /locus_tag="OO575_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6666298.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8105..8962
                     /gene="murI"
                     /locus_tag="OO575_003464"
     CDS             8105..8962
                     /gene="murI"
                     /locus_tag="OO575_003464"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6666299.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2093 a         2219 c         2475 g         2380 t
ORIGIN      
        1 tctgggtatt ggcgaagcgc catttatgcc tgcgggagta aagtcgatca ccgactggta
       61 tgcacaaaaa gaacgcggca cggcgctggg gatttttaac tcatctaccg ttatcggtca
      121 ggctatcgcg cctcctgctc ttgtattgat gcagctggca tggggctggc ggacgatgtt
      181 cgttatcatc ggcgtggcag ggattctggt tgggatctgt tggtacgcgt ggtatcgcaa
      241 ccgggcgcag tttgtcctga ctgacgaaga acggacgtat ctctccgcgt cggttaaacc
      301 gcgtccacag ctgcaattta gcgagtggct ggcgctgttt aagcatcgga caacctgggg
      361 gatgatttta ggtttctctg gcgtcaatta taccggttgg ctttacattg cgtggttacc
      421 cggttatttg caagccgaac aaggtttcag tctggcgaaa accggttggg tagcggcgat
      481 tcctttcctc gcggcggcgg tcgggatgtg ggttaacggt attgtggtcg atcggctggc
      541 gaaaaaaggc tacgacctgg cgaagacgcg taaaacggct attgtctgcg gtttgatgat
      601 gtcggcatta ggcacgttgc tggtcgtgca atcttcctcg ccagctcagg cggtagcgtt
      661 tatctcaatg gcgctgttct gcgtgcattt cgcgggaacg tctgcatggg gactggtgca
      721 ggtgatggtg tcagaaacaa aagtggcttc catcgccggg attcaaaact ttggcagttt
      781 tgtttttgct tcctttgctc cgatcgtaac cggttgggta gtggatacca cacactcgtt
      841 taatctggcg ctggttatcg cggcctgtgt gacgttcacc ggggcgctgt gttacttctt
      901 tattgtcaaa gatcgcattg agtaatcgtt actatcaggc tctcttgctg tgggcctgta
      961 gaataaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt agttattcga
     1021 gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc cgctccgttt
     1081 ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc tattctacct
     1141 atcgccatga actatcgtgg cgatggagga tggataatga atattcgtga tcttgagtac
     1201 ctggtggcat tggctgaaca ccgccatttt cggcgtgcgg cagattcctg ccacgttagc
     1261 cagccgacgc ttagcggcca aattcgtaag ctggaagatg agctgggcgt gatgttgctg
     1321 gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt ggatcaggcg
     1381 cgtaccgtgc tgcgtgaggt gaaagtcctt aaagagatgg caagccagca gggtgagacg
     1441 atgtccggac cgctgcatat tggtttgatt cctacggttg gaccatacct gctaccgcat
     1501 attatcccga tgctgcacca gacatttcca aagctggaaa tgtatctgca tgaagcacag
     1561 acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat cctcgcgctg
     1621 gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct
     1681 atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac tgatggccga tctggcaggg
     1741 gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat gggtttctgc
     1801 tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga aactctgcgc
     1861 aacatggtgg cggcaggtag cgggatcact ttattgccag cgctggctgt gccgccggag
     1921 cgcaaacgcg atggggttgt ttatctgcca tgcattaagc cggaaccatg ccgcactatt
     1981 ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc
     2041 atccgcgcaa gaatggatgg ccatttcgat aaagtgttaa aacaggcggt ttaaaccgtt
     2101 taacgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg tgttgacgaa
     2161 gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga tatgaataat
     2221 ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt cccgatggaa
     2281 caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcacgtgcca gatgtttgaa
     2341 ctgggcgcgg cccacttcat acggcacttt catcgcggtc agctgctgtt cggttttgcc
     2401 cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa tcagatgtgc
     2461 ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga
     2521 tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg
     2581 atacatgctg ttgaccttca gctgtccgcg actgtcagtt tccagcccaa tgttctgtaa
     2641 cgccagcgaa tcggtattac cggtacgacc gttggcatag agcaggcagt cagctttcag
     2701 ttttttaccc gacttcagat gcatgatcac accgtcgtca cagctttcga tcttctcgta
     2761 ctcttcgttg tgacgaatca ctacgccact gttccagaag tggtaggaga gagaatctga
     2821 catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat ccacttttac
     2881 atccataccg cggaagatcg acgcatattc acagccgatc actccagcac catagataag
     2941 tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg
     3001 ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa caaatttttc
     3061 agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac
     3121 aaagcgagcg ttcccctgca atatttcaca gtgattacgt tcgtaaaatc cctgacgcat
     3181 gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa aagaagagcg
     3241 gagcagtcgg gaatggtcgc tgtatagtgg gttttgattg aattctataa tgcggctgac
     3301 ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc cgccaacatt
     3361 ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca ttgcagcgcc
     3421 ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat gtggcatggt
     3481 agggcttacc tgttcttata cataaaagca acagaatggt aacattctat cgcgggtaag
     3541 ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt tgtctgtttc
     3601 ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt ttaagcggca
     3661 ggtttccgct gtacgtaaaa gaaccggcca gtaaatatgt tttattgcgt taccgttcat
     3721 tcacaatact ggagcaatcc agtatgttca ttctctggta tagtgccagc agtacttttg
     3781 gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca aaaagaaaaa acccgccgtt
     3841 cgctggtgga agccgcattt agccaattaa gtgctgaacg cagcttcgcc agcctgagtt
     3901 tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg catttccgcg
     3961 acgtagacga actgggtctg accatggttg atgagagcgg tttaatgcta cgccaactca
     4021 tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt gatccgtacc tcggtctcca
     4081 catttatgga gttcatcggt aataatccta acgccttccg gttattattg cgggaacgct
     4141 ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac ttcattgcgg
     4201 aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact gaagcgcaag
     4261 ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac gtcggcgtcg
     4321 aacaacgtcg gcaattagaa gagcgactgg tactgcaact gcgaatgatt tcgaaagggg
     4381 cttattactg gtatcgccgt gaacaagaga aaaccgcaat tattccggga aatgtgaagg
     4441 acgagtaatg aaacaagcaa atcaagatag aggtacgctg ctgctggcgt tggttgctgg
     4501 tttatcgatt aatggtactt tcgcagcgct gtttagctcc attgtgccat tttctgtatt
     4561 cccgattatt tccctggtgc tgacggttta ctgcctgcat caacgttatc ttaatcgcac
     4621 catgtcggta ggcttgccgg gtctggcagc tgcctgtttt attctcggcg tactgctgta
     4681 cagcacggta gttcgtgcgg aatatccgga tatcggctct aacttcttcc cggcagtact
     4741 ctccgtcatt atggtgttct ggattggcgc gaagatgcgt aaccgtaagc aggaagttgc
     4801 tgagtaatcg gtatcatgcc ggatgctggc gcatccggca tgggttttac ttcgcggtca
     4861 gtaatacgcc gcactccata tggtgcgtgt aggggaactg atcaaacaga gccagacgtt
     4921 cgaccttgtg cgtctggctt aatgtttcca gatttttgca taacgtttcc ggattgcagg
     4981 agatatacag aatacgtgga tacgcctgca ccattttctc ggtttcactg tccagaccgc
     5041 tgcgcggtgg gtcgacaaaa atggtttcga actgatagct ctttaagtcg atcccttgca
     5101 ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc gccatacgaa
     5161 taatttgtac gttatcaata tggttagcgg caatgttgta ttgcgcggca gcgactgacg
     5221 gtttggcgat ttcggtggct aatacccgat caaaattgcg cgccagcgct aatgaaaagt
     5281 taccgttgcc gcagtacagc tccagtaaat cgccttttga gcctttggtt acgtccagcg
     5341 cccattccag catctgaata ttcatcgccg cgttcggctg ggtaaagctg ttttctacct
     5401 gacgatagat catctctttc cctgcgaccg gcagacgctc gtcgatgtaa tcttgatcca
     5461 gcgcgatttt ggttttcgtt gcccgaccaa tcagatgcac attcagattc tgcgcgcgca
     5521 gtgcatcgcg cagtgcttcc gcttcctgac gccactcatc atccagcttt ttatggtaca
     5581 gcagggaaac caccgcctga ttactcagcg tggtgaggta atcaatctgg aacaacttgt
     5641 ggcgcagaac gggattatta cgcacacccg caatcatcgc cgtcatcaac tggttgatga
     5701 gttcactggc ggcggggaag ctatccacgc ggatgcggct tttggtttgt tgatcgaaaa
     5761 tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa ctccgcgcgc atccggtaat
     5821 gactgaccgg cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc atcatacttt
     5881 gcaaacgtac cactttttcg gctaactgcg cttcatactg ttctgttgga aggtgttcgg
     5941 gggtcatgat gtatcctgaa aaattaaagt acgcggcgat tgtagggatt gctcatcaga
     6001 tgtccagatc ttgatgaatt cctatttgtg agctacgtct ggacagtaac ttgttacaac
     6061 ctgtagcatc cacttgccgg tcctgtgagt taatagggaa tccagtgcga atctagagct
     6121 gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc catccggtgg
     6181 gaagtcatca tctcttagta tcttagatac ccctccaagc ccgaagacct gccggccaac
     6241 gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc cttcttctat
     6301 tgtggatgct ttacaatgat taaaaaagct tcgctgctga cggcgtgttc cgtcacggca
     6361 ttttccgctt gggcacagga taccagcccg gatactctcg tcgttactgc tatccgtttt
     6421 gaacagccgc gcagcactgt gcttgcacca accaccgttg tgacccgtca ggatatcgac
     6481 cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt cgatatcacc
     6541 caaaacggcg gttcaggtca gctctcatct atttttattc gcggtacaaa tgccagtcat
     6601 gtgctggtgt taattgatgg cgtccgcctg aatctggcgg gggtgagtgg ttctgccgac
     6661 cttagccagt tccctattgc gcttgtccag cgtattgaat atatccgtgg accgcgctcc
     6721 gctgtttatg gttccgatgc aataggcggg gtggtgaata tcatcacgac gcgcgatgaa
     6781 cccggaacgg aaatttcagc agggtgggga agcaatagtt atcagaacta tgatgtctct
     6841 acgcagcaac aactggggga taaaacacgg gtaacgctgt tgggcgatta tgcccatact
     6901 catggttatg atgttgttgc ctatggtaat accggaacgc aagcgcagac agataacgat
     6961 ggttttttaa gtaaaacgct ttatggggcg ctggagcata actttactga tgcctggagc
     7021 ggctttgttc gcggctatgg ctatgataac cgtaccaatt atgacgcgta ttattctccc
     7081 ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa gttgggacgc cgggctgcgc
     7141 tataacggcg aactgattaa atcacaactc attaccagct atagccatag caaagattac
     7201 aactacgatc cccattatgg tcgttatgat tcgtcggcga cgctcgatga gatgaagcaa
     7261 tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg gtagtattgg tgcgggtgtc
     7321 gactggcaga aacagactac gacgccgggt acaggttatg ttgaggatgg atatgatcaa
     7381 cgtaataccg gcatctatct gaccgggctg caacaagtcg gcgattttac ctttgaaggc
     7441 gcagcacgca gcgacgataa ctcacagttt ggtcgtcatg gaacctggca aaccagcgcc
     7501 ggttgggaat tcatcgaagg ttatcgcttc attgcttcct acgggacatc ttataaggca
     7561 ctaaatctgg ggcaactgta tggcttctac ggaaatccga atctggaccc ggagaaaagc
     7621 aaacagtggg aaggcgcgtt tgaaggctta accgctgggg tgaactggcg tatttccgga
     7681 tatcgtaacg atgtcagtga cttgatcgat tatgatgatc acaccctgaa atattacaac
     7741 gaagggaaag cgcggattaa gggtgtcgag gcgacagcca attttgatac cggaccactg
     7801 acgcatactg tgagttatga ttatgtcgat gcgcgcaatg cgattaccga cacgccgttg
     7861 ttacgccgtg ctaaacagca ggtgaaatac cagctcgact ggcagttgta tgacttcgac
     7921 tggggtatta cttatcagta tttaggcact cgctatgata aggattactc atcttatcct
     7981 tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc gtatccggtc
     8041 acctctcacc tgacagttcg tggtaaaata gccaacctgt tcgacaaaga ttatgagaca
     8101 gtctatggct accaaactgc aggacgggaa tacaccttgt ctggcagcta caccttctga
     8161 accacgtccc accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg tctatgacga
     8221 gatccggcat ctcttaccgg atctccatta catttatgct ttcgataacg tcgctttccc
     8281 gtatggcgaa aaaagcgaag cgtttattgt tgagcgagtg gtggcaattg tcaccgcggt
     8341 gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc aacaccgcca gtaccgtttc
     8401 acttcctgca ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc cggcgattaa
     8461 acctgctgca cgtctgacgg caaatggcat tgtcggatta ctggcaaccc gcggaacagt
     8521 taaacgttct tatactcatg agctgatcgc gcgtttcgct aatgaatgcc agatagaaat
     8581 gctgggctcg gcagagatgg ttgagttggc tgaagcgaag ctgcatggcg aagatgtttc
     8641 tctggatgca ctaaaacgta tcctacgccc gtggttaaga atgaaagagc cgccagatac
     8701 cgttgtattg ggttgcaccc atttccctct actacaagaa gaactgttac aagtgctgcc
     8761 agagggaacc cggctggtgg attctggcgc agcgattgct cgccgaacgg cctggttgtt
     8821 agaacatgaa gccccggatg caaaatctgc cgatgcgaat attgcctttt gtatggcaat
     8881 gacgccagaa gctgaacaat tattgcccgt tttacagcgt tacggcttcg aaacgctcga
     8941 aaaactggca gttttaggct gatttggttg aatgttgcgc ggtcggaaaa ttattttaaa
     9001 tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca cggaacaacg
     9061 gcaaacacgc cgacgggtca gcggggttct cctgagaatt ccggcagaga aagcaaaaat
     9121 aaatgcttga ctctgtagcg ggaaagcgta ttatgtacac cccgcgc
//
LOCUS       DAJTYM010000129         9141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTYM010000129 DAJTYM010000000
VERSION     DAJTYM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..278)
                     /locus_tag="OO575_003465"
     CDS             complement(<1..278)
                     /locus_tag="OO575_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCR6666300.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQ"
     gene            complement(275..1153)
                     /gene="xdhB"
                     /locus_tag="OO575_003466"
     CDS             complement(275..1153)
                     /gene="xdhB"
                     /locus_tag="OO575_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6666301.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1164..3461)
                     /gene="xdhA"
                     /locus_tag="OO575_003467"
                     /pseudo
     CDS             complement(1164..3461)
                     /gene="xdhA"
                     /locus_tag="OO575_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            3876..4631
                     /locus_tag="OO575_003468"
     CDS             3876..4631
                     /locus_tag="OO575_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6666302.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4710..4783
                     /locus_tag="OO575_003469"
     tRNA            4710..4783
                     /locus_tag="OO575_003469"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4742..4744,aa:Gly,seq:ccc)
     gene            4918..6528
                     /locus_tag="OO575_003470"
     CDS             4918..6528
                     /locus_tag="OO575_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6666303.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6560..7167
                     /locus_tag="OO575_003471"
                     /pseudo
     CDS             6560..7167
                     /locus_tag="OO575_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8098..8844
                     /locus_tag="OO575_003472"
     CDS             8098..8844
                     /locus_tag="OO575_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6666304.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2335 a         2248 c         2339 g         2219 t
ORIGIN      
        1 tgctgaacat gagaaagttt tccgcctttc gcttcacctt ccagcgtgcg gatctctttt
       61 ccttcagccc aggcggcaag gtataagcaa ctgtctattg ctgtgccgtc gaccaacacc
      121 gtacaggcac cacattcacc cacgcagcac ccttgtttga cacttagcag tccttgttcg
      181 cggagtaatt ccgagagcgg cgtgcctggt gcggcgtgaa gctgaaaagg catcccgtta
      241 atggtgcatt cggtggtaat tgtttcgctg tgattcattg caattttccc cccgccgcgg
      301 cgacggcttc gctaatcact tttttggtca tcgtctggat gagatgcaga cgaaactctt
      361 tactggcccg ccatgaagaa cgcggggcga catcttgcag gacagattcg ctgatagctt
      421 ccagcgtttg caggtttaat ggcgcatttt gtgcagtctg ttcggcatgt tggcagcgaa
      481 tcggcgttgg cgcggcaaca ccaaatgcca ggcgtaattc gctgaaattg ccgttatcca
      541 gtcggcaatg tgcggcgcag ccaatcgttg aaatatccat tgcgtcgcgc atggcatatt
      601 taaaatgcgc gctgcccgcg tgttctttcg gctgtggcgg aaaatgaaag gcgacgagga
      661 tttcgtcatg ctcaagagac actttgcccg gcccggtgtg aaagccatta atcgggacga
      721 aacgaacacc gcgcggggag tggatctcca gtttcgcgtc ataaattagc gttggcgtgg
      781 cagaatctgc gctggtggca ccgttgcaaa tatttccacc gtaggtagcg acgttacgga
      841 tctgcggtcc agcaatgaac gtggccgcag cacataacgc cgggagatga cgttgagtta
      901 taggatcttc tattagctgg gtaaatgtcg ttgcagagcc gatacgtagc gagccatctt
      961 ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata tggcgataac
     1021 ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc agtttggcct
     1081 gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga tggtaagaag
     1141 caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc ttcatataac
     1201 cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt caccgcgtta
     1261 cgaatagcag cggcaacagg aattatgggt ggctcaccca gtgacttatg tccgtatgcg
     1321 gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc cggcatggtc
     1381 ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc atcgatgatc
     1441 atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac ctgaccttct
     1501 gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg gttgatggtg
     1561 actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa cgccggtggg
     1621 ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc agggtggtag
     1681 aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg ttcaaccagc
     1741 acgatatggc cttttatcag ggtcaaattc atcgctgact gatgtagcat gactgcggcg
     1801 tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg cgcggcaaca
     1861 tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt atcttgtgtt
     1921 gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat ttgcgagaag
     1981 acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat ggttccatcc
     2041 tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt gttagaggtg
     2101 tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt ctggcattct
     2161 gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc cggcaaccct
     2221 gcgctgcaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc ggcgttgcgt
     2281 aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag catagactca
     2341 acggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga ggggaggttg
     2401 gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta agcgacttta
     2461 ttcccccccg cagaagcgat ggagtgcccg tgagatgcat aagcgccggt gttagacaga
     2521 acatccagac tataaccttt caaggttccg tcgcggttca cgcccatttg cccgtcaata
     2581 gtaaaagcgt ggcgggtacg ggttgcgagg aaacactctt cacggctaag ggaaactttc
     2641 accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc cagtacatcc
     2701 tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca tgaccaggga
     2761 atatccagcg cctgaccaac cacgcggcga acaatgtgcg ggatctgggt gctggaaacg
     2821 atggtaattc gcgagtcatc ctccatccac gccagcgatg ttacgctttc catgtgacaa
     2881 tgttgaataa cgggagtctg atagtgcccc tgtacctggt agtcggcggc atcgattgtt
     2941 tgttggacat tgcccgtcga catcgtgctt tgtttcagta aattgccgcc gttatggatt
     3001 ggtgcagcgt cttctgtcag cgccgcttct ggcgtggtga taacgggtaa ttcttgccac
     3061 tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcgcgggc cacgacgatg
     3121 gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc ggcggtatcg
     3181 cgcttgtttt cgtcaagtgt ctaggcatgc ccagctgtag cgaatggaat atcaggcaca
     3241 tcttcccaag taaaaatcgc cagtacgccc ggtaaacttc tggcttgttc atcattaata
     3301 cttacggcat aaccatgtgc gatagggcta cgtacatatt tcgcgtaaca catgcccgcc
     3361 ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatggc atcgacgcgc
     3421 atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc ctcgatttga
     3481 acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc cagaaacgac
     3541 gataagcgtt aggggaggaa ggaagtgtga tgacgttaaa aaaacacaga tatgatacga
     3601 aacaggactc tgaataggga cagtgatacg tatcaatatg agtggcgata aaatcaatgt
     3661 gataaatcag gattattacg catcaggatg ggaaataggg taaaaagtca ggaacttcga
     3721 tacctctaca gcgaatattt tgctgatgat acttcccttt gccaaatgaa attaacctct
     3781 ttctctatta ccacgttttt ccagaagcaa gagattgggt atcaagaggc tggctgctat
     3841 gatatggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc ctgaataaaa
     3901 aatctctggg tatcgtgatg tttttatcgg ttggactgct attggcgggc tgttcgggta
     3961 gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa cggggggata
     4021 cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agagctggcg cgactgaacg
     4081 gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt ggggcgaaaa
     4141 gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac taaaaccgca tcggttacac
     4201 cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt tggttatggc
     4261 caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat aaagggattg
     4321 atatctcagc tccacggggt acacctattt acgccgcggg tgcaggaaag gtggtgtatg
     4381 tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt gaagattaca
     4441 ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc gtgaaggctg
     4501 ggcaaaaaat cgccactatg gggagcacgg atgcggcatc tgttcgcctg catttccaga
     4561 ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgccgcag ggcagcaagc
     4621 caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta aggagagcgt
     4681 aaggtttata atgccttacg catctcgaag cgggcgtagt tcaatggtag aacgagagct
     4741 tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccatgacact tcttaaagtc
     4801 tactaactta ctgaatccta agggatacgg cttaacgcct tccgatagtt ttactcactg
     4861 taaccaacac tgtgactaac tttgttgaag tagtgagcag acctaaggtc tcagtacatg
     4921 ctcaagtcaa gaacctacct ttatcagcgt aatggtgtct tttacatccg tcttaggatg
     4981 aagaccacta gtcgcctgac cgcttcgctt ccttctcaca accgttataa actagcgtca
     5041 gtatctttac ggaccaagga tagacgtacc gctatggctc actcacggca catcaagtca
     5101 gcacttaaag caatccatgc agacaaccct aacgcctctt atgaggagct acgggagcac
     5161 ctgaagacca ttgtcgagtg ggaacttagt gtcagccgtg atgacctgaa cgacccggag
     5221 tcctaccagc tttacgttga ccagtacgat gacattaagt ccaaccttcg ggaagctgta
     5281 gcgaccgagc gtctgactgt agaccagcat cgctatatta atgacgttat cggtgtgctt
     5341 aaggcctgtc aggatagact caatggcgat agctctggcc tattgtctta ccttgagcct
     5401 gagactggca gtctaagacc gtccgtctcc ctatctgtat tggcggaacc tgaagtccct
     5461 gaacctaaag cactgaccct cgcgtctctc attgagcagt acgagcagga gaacgcccag
     5521 aactggaagc cagcgaccct aagcgaaaac cgagcgtcac actctacgct aatcgagata
     5581 ttcgactatc tggatattca ggatgtcggc aaggcgaccc gtgcagatat gctcagagtt
     5641 cgtgaagtcc tccagcagct accgaagaac cgcaagcaac gctttaagtc tatgccgctg
     5701 tctgacttgc tgaaccggga gtctaagact gactgtctgg acgtcgttac cattaacaat
     5761 aaatatctta tcaagatggc tgctgtgttt aagtgggcgg tacgcaacga cttgatagct
     5821 aagaacctga ccgaaggtct tgagctgaaa gtccctcagc gtaaagcctc cgacgctcgt
     5881 gatgcgttca gtcctgagca ggtagggcaa ctactggtcg cagctaaagc gtactcgcag
     5941 aagacctcag gtaagccata ccattattac gtcaccgctc tggctgcgat tacaggtgcg
     6001 agacttaacg aggtggccca acttcaggtt aaagatgtca ggaccactga ggctgggacc
     6061 gtttttatcc atatcaacga agacgatagc agcctgccgg gtaagagtat caagaacgcc
     6121 cacagtgacc gctgtgtccc gttggtagat ggtgcttatg gttttgtatt ggctgacttt
     6181 atgtcactcg tggaagaccg tagaaagact gagggtgata atgctatggt gttcaatggc
     6241 ctcaagctaa tgaagaacgg ctacggtgag caggttagca agtggtttaa ccgaacgttg
     6301 ctgcccaaag tcctcgctga ccgtagcggc ttagcttttc actcgtttag acatacagtt
     6361 gcgacccagt taaaacaaca tggggtcgag ttagcgtatg ctcaggcgat tatgggtcat
     6421 agctcagggt ctataactta cgaccgttac gcgaaagagg tcgaggtgga aacgctgaaa
     6481 gaaaaattag cggagtcatt gtcagtaaaa aaaatagacg gaaagtaatc gcatagggat
     6541 agcggaatgt gaggacgtaa tggtcaaaga atttttagtt agcaaagaaa ataagccttc
     6601 atatgaatgt atgaatactg ctgaaacttg tttggggctg gctggaatgg cttcggaaga
     6661 ttggcaacgt gagcatcatt gccgcacagg tataatattt actgcttttg ctattgaggc
     6721 aatgttcatc ttctatagaa aacaggttga ccccggctac gataagacgc agaaagagtg
     6781 cagaaagaca atgcacaaga atacgttaaa attgtgcggt ataaacaatt atatggggac
     6841 aaaaccttac caaattatca aagagtgtct tgaggtaaga gacgctattg ctcacggcga
     6901 ttcgtatact tcgagcttta atttctcggc ggatcatttg gataaccaag acgatattgc
     6961 aaaagtgtcc tggcggtaaa ctctgaacaa tttcggggat taactgttga gcgccttgca
     7021 aaatacattg aaatggcaaa gcatattgac catgagattt ctgataatgg ttacaggcct
     7081 tctgaaggtt accttccagc agccgagcgc agaagtctga tgttagcttt tggcgtcagt
     7141 ggtgtgtcaa tgtggcccag tgcttgagcg ataatacacc tcactataga ggaatggacc
     7201 cttaactatc actatagggg agagacccta agtccccacc ttaatagcag tcacgaccca
     7261 tacacacatt cattaagtct caggcttccg gtagcggacc gattggaaac catccgcaga
     7321 cgcctagtgt taaatcacct tggcctgaga ccattccttt taacgtaagc tatcttccct
     7381 tttgacaggc ggggagcagt agaccacatc cgactttaag ttggctctgt gataatcgct
     7441 gcgcggataa ctgaagaacc aagacaccat tactgaggtc tgccatccat ttgtggacag
     7501 gcggaaactc aaagaggtta gatcagtggt gtctgaggtt ggagctttga gtcttgacca
     7561 gcaggctgga ggagaccatg agtttatctt gttatggatt gtgttatcac aattccaata
     7621 agaagaactt taagtccagc ctccactccc gccaacccga agtaaaatta tggtcgctat
     7681 gatgtggtct ccatgcttcg ggtcttactg gaagtactga acactttcgc gtcaagttct
     7741 ccgaagttca ttcctgtttg actaaactat cactataggg accatatctc gcctctgacc
     7801 tcctgagaga cggcaagcga gaaaccgtgg tccacgctag caagctagcc accagcctaa
     7861 ggcaagacag cctctggtca gcctgtgtgt tctttggtct cgtgacttaa gtctatcacc
     7921 acacacgctc ggacatgctg tcgaaccttc atctctatgg tcatcgcaga ccactaaccc
     7981 caattacctc aagtcattct gtttgcgtcc tgttgcctgc tggtctccct ttacgtgggg
     8041 gcttgagtag gtcagtgggg tgcgacttca gggtgcaaac aaaaggagta cacgtaaatg
     8101 tcccaacaag aaatcagcat cattaacctc gaccagcttg tgtccatgac ctcagttgaa
     8161 attgctgagc tgacaggaaa agaacataaa cacgttttac gagatattcg taatatggtc
     8221 gaagagctta acggggccaa aactgagcac tgctcaactt tgagcagcga gcttaacggg
     8281 tccaaatttg ggctcgttgg cgaggaagta tacaaggacg cgaaaggtga gagccgaaca
     8341 atgtatcgtc tcgaccgtaa gcatactttc attctggtcg ctggctattc cgttcacctc
     8401 cgcgccaagt gttacgacca tattcagacg ttagagcgtc gagtccttca actggaagac
     8461 cagaagaagc gagcagccat tcagtccgct aaccgccgag gcgtgacttg gggcgactac
     8521 tgcaaaacct atggtctgcc cgctcagaag ctaatgaccg cacttctcca gcatcgtggc
     8581 ctctttcgta agaacccaat cagtaacgag tggtctgtaa atccgaagta ttccgactgc
     8641 tttcgtatca ttaagccgtc agaccagaag ttctcagcag gtgggtataa cttccggttc
     8701 aacgctaagg ggctggaggt cttcggtaag cctgagatgg ttgacaagat gcgaggcatc
     8761 cttattgcat tcactggcac cgaccagcag aagcaagagc acctcctgaa gctggcacaa
     8821 tccggtaagg tggagggaat ctaagatgat gattgacccg cgcacacctg aggggcgcat
     8881 gactctccgt taccgtggct accggactga agtcctcctc agagagcttg gtctagaccc
     8941 ggaagatgag acccgccagc accaatcccg agacgagcta atagcccaac tggtcgctat
     9001 gaaactgccg cttaaccgtt agtagctcac ctactcaata cctaatttaa acaccttttg
     9061 agatgaccga tgcggggaga cccgtaggcc ttactgtgcc tgcgataccg atagtctcaa
     9121 ctctcaagag gagaattacc t
//
LOCUS       DAJTYM010000130         9081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTYM010000130 DAJTYM010000000
VERSION     DAJTYM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1356)
                     /gene="phoA"
                     /locus_tag="OO575_003473"
     CDS             complement(<1..1356)
                     /gene="phoA"
                     /locus_tag="OO575_003473"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6666305.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VV"
     gene            complement(1457..1717)
                     /gene="iraP"
                     /locus_tag="OO575_003474"
     CDS             complement(1457..1717)
                     /gene="iraP"
                     /locus_tag="OO575_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6666306.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(1899..2102)
                     /locus_tag="OO575_003475"
     CDS             complement(1899..2102)
                     /locus_tag="OO575_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666307.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2180..3274
                     /gene="ddlA"
                     /locus_tag="OO575_003476"
     CDS             2180..3274
                     /gene="ddlA"
                     /locus_tag="OO575_003476"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6666308.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3298..3510)
                     /locus_tag="OO575_003477"
     CDS             complement(3298..3510)
                     /locus_tag="OO575_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6666309.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            3770..4078
                     /locus_tag="OO575_003478"
     CDS             3770..4078
                     /locus_tag="OO575_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6666310.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4137..5231)
                     /gene="yaiW"
                     /locus_tag="OO575_003479"
     CDS             complement(4137..5231)
                     /gene="yaiW"
                     /locus_tag="OO575_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6666311.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5244..6464)
                     /gene="sbmA"
                     /locus_tag="OO575_003480"
     CDS             complement(5244..6464)
                     /gene="sbmA"
                     /locus_tag="OO575_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6666312.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            6816..7973
                     /gene="ampH"
                     /locus_tag="OO575_003481"
     CDS             6816..7973
                     /gene="ampH"
                     /locus_tag="OO575_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6666313.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(7974..8477)
                     /locus_tag="OO575_003482"
     CDS             complement(7974..8477)
                     /locus_tag="OO575_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6666314.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(8565..>9081)
                     /locus_tag="OO575_003483"
     CDS             complement(8565..>9081)
                     /locus_tag="OO575_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6666315.1"
                     /translation="SHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDAGYV
                     TPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPYFKL
                     AYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQD
                     WSANVGVKYTW"
BASE COUNT         2263 a         2317 c         2165 g         2336 t
ORIGIN      
        1 aacaacattg gcggcatgcg ggccatacgc cgcaatacgc aactgactgc cggtatgttc
       61 ttgtgaatcc tcttcggaat tcccgtaact catcaccatc actgcgccat ctttagtatt
      121 tagcgcctga gtgaggcccg gcgctttggt atccggcgca acaacctggc tggcgtgggc
      181 gtgatcagcg gtgactatga ccagcgtgtt accctccttt ttagcgaatt ccagcgcccg
      241 ttgtacggct tcatcgagat cgaccgtctc gccaatttgc ccacaagggt tcgcagcgtg
      301 atcctgttta tcgattgacg caccttcaac ttgcaggaaa aatcctttct catttttact
      361 caacaattca atggctttgt cggtcatctg tgccagggtt ggtacactgt cattacgttg
      421 cggattaggc gtataggtga ctgcgggctt atcgatattg ccgtggtacg ttgctttcgg
      481 ccctagccag cgcactggca tattgccgtc agcaaacagg ccaagcaggg gtttttgctg
      541 attcgcttcc gtcaccgaat tcagtgaggc agcatcgttc accaactgat aaccacgcgc
      601 ctgtgcctgt tcacgcagcg tttttccctg ccattcacca gcggttgccg tttcagcaaa
      661 ggtttttgcg ccgccgccaa gcgtaacgtc ggcacgagcg ttaagcagct gttcggtaat
      721 cgatcctttt ccgccttttt ccagagcgtt acccggacat ttttcactgg tcgcgctcgg
      781 accgtagcat ttgcgcgaag tcacatgtgc caccagcgca gcgggcgtgg catcctgcaa
      841 ctctgcggta gaaacgttac cggtcgccag acctgcggct tttgccattt ccagaatcgt
      901 tgggtgatct ttttcgtgaa tatcgacgcc cagcgcgccg ttataggttt tgacatcggt
      961 tgaccaggcg gttgctgatg cagccgagtc ggtgacgtag tccggtttgc cagttttttt
     1021 attcagcgca tagtgagtgt attgcccggt aagcggtaag gcatcgatac ctttaaaaaa
     1081 gccgcccgca ccttcggcat aattacgtgc ggcagtaatt tccgaatccc ccatcccatc
     1141 gccaatcagc aaaataatat tttttgcagg tttatcgcta agagaatcac gcagagcggc
     1201 ggtctgatca cccgttaagc ggcgagcacc gccgggtgca gtaatatcgc cctgagcagc
     1261 ccggttttcc agaacaggca tttctggtgt ccgggctttt gtcacagggg taaacagtaa
     1321 cggtaagagt gccagtgcaa tagtgctttg tttcacttta ttttctccat gtacaaatac
     1381 attaaaaaat aaaaacaaag cgactataag tctcggccgt gacaacttta tgacagctgt
     1441 tgaaaagatt aactttttac tgacaaggat gcttcaataa cttctttacg taatcgcgca
     1501 gcagctctgt atcgtcgtca ggaatgctgg catcgggctt tacctcgtac agcgccccct
     1561 ctacctgatc aatcaaccgc tgttggtcat tttgcgccat attgcgaagc attgcagtga
     1621 cgataatctc caaagcttct acctgcgcac acagttcttt cgactcttct tctttttggg
     1681 caagcttaaa taacaactca gcaatgagat ttttcatgtc tgtatttcct tatccaaagt
     1741 atggagaagt taacattatg tttcacaact tcatagtgtc tggaagtatt atttttatga
     1801 caaaggaaat actttgcgca aatatttttt gtttgattgc cagcgaaacg tttctcttat
     1861 ttaaataatt acccacacaa aatataggct tttaattatc aacctttatt ataaataaaa
     1921 gctatgaaat atattctctt cgttaactta taattaagag aaaaaaactc accgcattgc
     1981 catttaagcc aggaataaat tagcttcccg ttcatcattt ttgtgatctt gcgcacatct
     2041 ttgagataac gcactttctt caccagtacg ttgcacgctt tgtcagcaga agattgcaac
     2101 aatctttcag aaggccgcga atcccccgtt ttgtcgtggt aatgtatgca ctttgtgtta
     2161 gacgggatag gtttttaaga tggaaaaact gcgggtagga atcgtttttg gtggtaaatc
     2221 agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt gtcgatgcca ttgataaaag
     2281 tcgcttcgac gttgtgctgc tgggcattga taaacaaggg caatggcacg tcagcgatgc
     2341 cagcaattat ctgctaaatg cagacgatcc tgcccatatt gcgttgcgcc cttcggcgac
     2401 cagccttgcg caggtgccag gtaaacatga gcatcagctt atcgacgcgc aaaacggtca
     2461 gccgttgccg acggtggatg tcattttccc gattgtccac ggtacgctgg gcgaagatgg
     2521 ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt gtaggttctg atgttctggc
     2581 ttcagcagcc tgtatggata aagatgtcac caaacgtctg ctacgcgatg ccgggctgaa
     2641 cattgcgcca tttattaccc tgacgcgcgc taatcgtcac aacatcagtt ttgccgaagt
     2701 ggagtctaaa ctggggttac cactgtttgt aaaaccggct aatcagggct cttctgttgg
     2761 tgtcagcaaa gtaaccagtg aagaacagta cgcaattgcc gtcgatctgg cgttcgagtt
     2821 cgatcataaa gtgatcgttg agcaagggat caaaggtcgt gagatcgaat gcgcagttct
     2881 gggcaacgac aatccgcaag ccagcacctg tggcgagatc gtactcacca gcgatttcta
     2941 tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa gtggtagttc cggcagccat
     3001 tgcaccagag atcaacgata agatccgggc gattgccgtt caggcttatc agacgttggg
     3061 atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca gagaacgaag tggtgatcaa
     3121 cgagatcaac acactgcctg gcttcaccaa catcagtatg tatccgaagc tgtggcaagc
     3181 cagcggtctg ggttacaccg atctgatcac acgtttgatt gagctggcgc tggagcgtca
     3241 cgctgcggat aacgcattga aaaccacaat gtaatatttt aacgcccgat aatattctta
     3301 ttgggcgttt tcagtttctt catcgcgacg acgaataatc aaccccgcca gccagaaact
     3361 aatcacccac gtcaccagac cgacggcata ggtctgccaa ccttttgctt caaatcccag
     3421 tacccccacc acgccattaa gaatgaatat aaggccaatg gcgaacgcat agtagtgcca
     3481 gtcacggcgg atttttacag gcagattcat ggcagctccg ttaacgaaaa acaacatcct
     3541 tgcatattct gtaacaaccg tctgtggtgt agacaacatt ttcgtaatta ttaataaatt
     3601 taacttaagg ttgccatttt gtgactcaca acgaagattc acgcttcggg aatattccca
     3661 acatcaattt accatgattc tgatattgac aatctaaatg acctgtcaga cttgccataa
     3721 gccgctggta tcacaccaga agagaagcag atgacttccg gaggttttta tggctgattt
     3781 caccctgtca aaatcgctgt ttagcggaaa atatcgcaat gcctcttcaa cgcctggcaa
     3841 cattgcctat gcgttgtttg tgctgttttg cttttgggct ggggcgcaat tgctgaacct
     3901 gttagtgcat gcgcccggcg tctatgagcg tttaatgcag gtccaggaaa caggtcgccc
     3961 acgggtggaa attggtttag gtgtcggcac cattttcggg ctgatcccgt ttttagcagg
     4021 ctgcctcatt tttgcagtgg tggcgctatg gctgcactgg cgacatcgcc gccagtaaaa
     4081 tttacgttgc cagatgcggc ctgaaacgcc ttatctggcc tgacattcct gtttgatcac
     4141 tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg caaaccaggc cgtagtcagg
     4201 ttgcgggtga ttttcgggct ttccagttga atgccaggca acatttcgcg gggtagtgat
     4261 ttacccgttt tcgcttcggc aagttgataa actttcttgt acagcgccgt ctcctcaaaa
     4321 gagaagctgt cacctttctc taactgacgg cgaatttcgc tgtcgctcat ccccagtttt
     4381 cccgccagtt tgcgcgttgc cagttcggtt ttcccgggtt ctttactgtc gtaacgaatt
     4441 aaatcaccgt ccagtgccag cttcacgccg ctggccttac tgactgcgtt ctgaaacgcg
     4501 gcattacggc tggcgtacca gccagcgtta aaatcagcaa agcggtatat cggtgcgcta
     4561 tagctggcgg gataattcag taaatggtaa gtaccaaacc acaacccgcc gcggcgactg
     4621 aagacttcct gacgaactgt accgtccatt ttccacggat accctttggt atgctgttcg
     4681 gcaaaagcaa tgctgacctg catcggccca ccggtgcgca ccggattaag cgaaccaaac
     4741 agcgtctgcc ccattggtac catgctgatc agatcgtcaa aaatcgcgct caattgcttt
     4801 tctgtacgca ccgaatctaa ccgttcgcta taacttttgc cgtttggcga tttgattttt
     4861 agcgcggtat gaaccagaaa agcaggaatg tgcatccgtt cagcacgacg gtcaatctct
     4921 tgccaggcga tcttgcttaa gcccggaacg gccggatctg cctgataatt tgactcctgc
     4981 tgcgccaccg ccagcaccga gtagacgttt tccagcgtcg gtgccagccc ctgactttca
     5041 aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt tcacgctcgc aggcatcttc
     5101 tgccgcacga cgctcgctac atctatcgct ttttcgccct ttttcaacgg ttgcggagcc
     5161 tgactactac accccgctaa aaccagaacc gccaacaatg acagcgaaga tagatgattg
     5221 acgcgcgaca ttacttctcc tttttagctc aaggtatggg ttacttcctg aattttgtca
     5281 ccatccagct catgttcaaa gctgcgcaga cgtttgtaga tagacatcaa ctcaaccagt
     5341 gtggtccatg agttaatcag gtactggaaa gcaccgcgaa cctgaccaaa aacgttggta
     5401 atctgcgtca tcaggccgag cgtaatcgta ccggcaacaa ttgacggaaa cagcaagaac
     5461 aaaccgaaaa cgttatcgac ctgcaaatag agaatgcggg cgatgttgaa atacatatag
     5521 tgaaaataga ggcgaaaata gtttttccgt acggcgctaa acagctcgcg taccgtaggc
     5581 ggcgtcgcgc gcgtggcatc gtcttcacca taaaccagct ctttacggta ggcggcctct
     5641 actcgctggt ttttaaactc cagccccggc agtttgatcc ctaccactgc cagcaatccg
     5701 gtccccatca gcgaccagac gatagcggca atcaccagac catacggaat gtgcccgata
     5761 atcggcagct ccggcacatg cgcggagagc gttaccagca ccggcaggaa ggcgatcaac
     5821 gtcatgatgg cgttgataaa actgacgccc atattctcca gcgttgaagc aaaacgcatg
     5881 gtgtcttcct gcacacgctg tgcggcacct tcgatatgac gcagttgttg ccagttcgcc
     5941 atgtaatatt cgttcatcgc cgtacgccag cggaacacgt agtgactgac aaagaagttg
     6001 ttcagtacac tgatcaccac cgcgatcagc gcaatcccca gaaagacgcc cacttcgcgg
     6061 taaaattgtt cgatagtgac tttatgcggc gaacttagcg cggtttgaat cagatcatag
     6121 aacggcgcat accaggcgtt gacggcgacc ccgacttcca ccaaaaacca agtgacgaag
     6181 ataatcagcg cagtaccgag tattgaccag tattgccaac ggtgcgggct gtagataaac
     6241 cagaaaaatg caaaaagtcc tacgcaaaca atgtagtaag cgtaaaaaat caggaaatcc
     6301 aacgaccaga aacgcgcggc gctaatcggg atctgcccgg aagcgccggt gatacgtgcc
     6361 acccagtcac ccccaccggc ttgccagaag ataacggcaa tcaatgccca aacaaaggcc
     6421 gagagaaaaa acgttcccgg ctttgggaaa aaagacttaa acatatgcac tcctgctaac
     6481 ttcttatcgt tttaacaact gctgtgtatt aaccgcatga ccgctacaaa gcgtcagcca
     6541 aatgaaaccg cccattgtga cccatagcaa ggcgcttagt tcgccagcct gaccataaga
     6601 attgtttcga cggatttcac aaaccaaaga ttgtttacat aagcggatta acacgcgcag
     6661 gatcacaaat gcaggcattg gtgagcagga aaaagatcgt gtttacgcgc cgttacgttt
     6721 ttaacactat tattgcacta attcgccctt tgcaatccat caatgagtag tataaatacg
     6781 ctcagttacc ttcattcaat ctatggacac caccgttgaa acgtagtctg cttttttctg
     6841 ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc gcagccgatc actgaaccgg
     6901 agtttgcctc tgatattgtc gatcgttatg ccgatcatat tttttacggc agcggtgcca
     6961 cggggatggc gctggttgtt atcgacggta accagcgcgt ctttcgtagt tatggtgaaa
     7021 cgcgacctgg taataacgtt cgcccgcagc tggattcggt cgtgcgtatt gcttccctca
     7081 ccaaactaat gaccagtgaa atgctggtga aattgctcga ccaggggacc gtaaagttaa
     7141 acgatccgct aagtaaatat gctccgccag gcgcacgcgt gccgacctac aatgggacgc
     7201 ccattaccct agtgaatctg gcaacccata ccagcgccct gccccgtgaa cagcccggtg
     7261 gcgcggcaca tcgtccggtg tttgtctggc caacgcgcga gcaacgctgg aaataccttt
     7321 ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc gtactctaac cttgcgttcg
     7381 acctgctggc agatgcactg gcgaatgcgt ccggcaagcc ttatacccag ttgtttgaag
     7441 agcagattac ccatccattg gggatgaaag acaccaccta caccccctca ccggatcagt
     7501 gccgccgttt aatggttgct gagcgcggtg ccagtccgtg caataacacg ctggcggcaa
     7561 ttggcagcgg cggcgtctat tccacgcctg gcgatatgat gcgctggatg cagcagtatt
     7621 tgtcatctga tttttatcag cgcagtaacc aggcagatcg catgcagacg ttgatttatc
     7681 agcgcgcgca gtttacgaaa gtgattggca tggatgtccc cggcaaagcg gatgcgcttg
     7741 gtttaggctg ggtatatatg gccccgaaag agggtcgtcc gaggattatt cagaagacag
     7801 gcggtggtgg cggtttcatt acctatatgg cgatgatccc gcagaagaat attggcgcgt
     7861 ttgtggtggt cactcgttcg ccgttgacgc gctttaaaaa tatgagcgat ggcatcaatg
     7921 atctggtgac cgagctaagc gggaataaac cgttggttat ccccgcatcc tgattaatac
     7981 tctgaaggca aacggttgat agcgaccagt ttgcctttat tcatttcgat atagccgcct
     8041 ttacgcaaag cagcgagaac ttctgcgacg acagaacgcg atatgcgtgt acgttgatgg
     8101 atataattca tcacaccaat acgtgatcgc aattcttcat tccagtcaat cattgataat
     8161 aatgttgcgc gggtttgttt ggctggtaga tgatatcccg tacaatttcc ttctgatatt
     8221 aatttgtatg gtatatcgtt tttcattaaa ccatcagcca gccccataat ataaggagcc
     8281 tgggtaatac cgataagtac gttttcttct ctacgcagag agataactcc ttctaaaata
     8341 acgaaagtat cttcgttatt gacatcactg gaaaatataa cttgcttttc attattaaac
     8401 tcgaagcgcg taccgtatct ggacaaacat ttatcgagct taccaaattc ctgaagaggt
     8461 ttaactacag ataacatttg cgcgtccttt gcagtaatgc ccgtcaaatc cttgacgggc
     8521 attatttaga ttaaattacc agtatttctt cggagtgaag aatattacca agtatattta
     8581 acacccacgt tcgcggacca gtcttgatct acgtcaccac caccgaggta gttagcatcg
     8641 gtataggcgc tgaagttctt ggtgaagctg aactgagtac ccagaccaac acgtaccgca
     8701 gacccttcag taccgttatc gatggaatca ccgttcacat cgttatcgtt gttagagtcg
     8761 tcgtagacgt aagccagttt gaagtacgga gtcagagcct gatcttcgct gtaggtgaag
     8821 gtataacctg catctacacc cagttcataa cgcatgctgt cgtaaggctg accgtcaact
     8881 ttcatgtcgt tgctcagctg gtagtcatca ccagactgga acagaccaga aatgctgccg
     8941 taaggagtca cgtaaccagc atcacccagt ttgaagtcgt aaccggtttt caaaccgaag
     9001 ccccaagcgt cggagttggt gctaccgtca acgtaagtac cgttgctcat ggttgcagac
     9061 aggtcgttgt tgaagtgaga g
//
LOCUS       DAJTYM010000131         9002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTYM010000131 DAJTYM010000000
VERSION     DAJTYM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO575_003484"
     CDS             complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO575_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6666316.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO575_003485"
     CDS             complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO575_003485"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6666317.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO575_003486"
     CDS             complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO575_003486"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6666318.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO575_003487"
     CDS             complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO575_003487"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6666319.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4439..5563
                     /gene="dprA"
                     /locus_tag="OO575_003488"
     CDS             4439..5563
                     /gene="dprA"
                     /locus_tag="OO575_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6666320.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5535..6008
                     /locus_tag="OO575_003489"
     CDS             5535..6008
                     /locus_tag="OO575_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6666321.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6037..6579
                     /locus_tag="OO575_003490"
     CDS             6037..6579
                     /locus_tag="OO575_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6666322.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6584..7156
                     /gene="tsaC"
                     /locus_tag="OO575_003491"
     CDS             6584..7156
                     /gene="tsaC"
                     /locus_tag="OO575_003491"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6666323.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7161..7979
                     /gene="aroE"
                     /locus_tag="OO575_003492"
     CDS             7161..7979
                     /gene="aroE"
                     /locus_tag="OO575_003492"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6666324.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7976..8233
                     /locus_tag="OO575_003493"
     CDS             7976..8233
                     /locus_tag="OO575_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6666325.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8209..8763)
                     /locus_tag="OO575_003494"
     CDS             complement(8209..8763)
                     /locus_tag="OO575_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6666326.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2249 a         2186 c         2248 g         2319 t
ORIGIN      
        1 ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa agggaaataa
       61 taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg gaagagtctt
      121 tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc gccttgctca
      181 atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc tccaataatc
      241 gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga ggtgctttca
      301 tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga ggaaacacca
      361 acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa
      421 attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg ctggatcaat
      481 accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat attggcctcg
      541 tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc ggccagcagt
      601 tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc cgccagttcg
      661 gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc cagacgacgc
      721 gccagccctg caccgatatt accgccacca accagcatga tccgtttata cggtttttcc
      781 agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat aaagaacact
      841 tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc gtggcggaaa
      901 attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga aagtgcatta
      961 ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt accctcagcg
     1021 aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt atcgataacc
     1081 aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa tagcttatcg
     1141 gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt gttgaaaagt
     1201 gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac agcaaccagc
     1261 atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc
     1321 acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt ggtatcgaca
     1381 acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc aacctggccg
     1441 gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt tttgattagc
     1501 ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt acccggttgc
     1561 tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa ggctttaatc
     1621 tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt tccaccggat
     1681 tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag ttccgggata
     1741 tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc cgttgctgaa
     1801 caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg ggaagggtaa
     1861 cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt gtcgtaaatg
     1921 cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg tgccacctca
     1981 aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg ttcaccgttt
     2041 tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt aacccatccg
     2101 tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac agcatcaggg
     2161 taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag ccagctgtcg
     2221 cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt attggcttcg
     2281 gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa ccaggaagga
     2341 tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg ccgacgctgg
     2401 aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat tgcgatagcg
     2461 ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag cagttgatac
     2521 aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg acgggccatt
     2581 aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa gcacaactct
     2641 tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag aatgttgctt
     2701 aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct acgtaaatta
     2761 cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag agtggactat
     2821 cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag gtcttgcgcg
     2881 ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc atcaccagtc
     2941 gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc gttggttgcc
     3001 gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc cagccagctc
     3061 attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga aagtgaccag
     3121 tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt ttcgtcctga
     3181 acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag cccttgtggg
     3241 ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt aatcgggcag
     3301 gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat aatggttaca
     3361 ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc gcgccagcgt
     3421 ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag cacagcttta
     3481 ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc ggcgaccagt
     3541 tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc ttcttcctca
     3601 gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg tcggtctggc
     3661 tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag atgacgcgct
     3721 gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt agttcttatc
     3781 cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt cgttgttgtt
     3841 tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc tcatgctgaa
     3901 tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg cggtcaaggg
     3961 cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg atcgacaggc
     4021 aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg attaacacca
     4081 gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg atatcaacct
     4141 gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata tcatcgacga
     4201 tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga agccgctcgt
     4261 ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt caggagttag
     4321 aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc acttgattga
     4381 cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg agataaggat
     4441 ggtcgatata gaaatttggc tgcgtttaat gagcatcagc agcttgtacg gcgatgatat
     4501 ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg tattgcagca
     4561 aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa agagtatcga
     4621 aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg acagcgaatt
     4681 ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg ttgaaggaga
     4741 actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc attcatggta
     4801 tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag tgacaattac
     4861 gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac aggtaaatgg
     4921 cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc gccatgcccg
     4981 actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc ccctcgatgt
     5041 tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc taagtaaagg
     5101 tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac gttgtgcgct
     5161 tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg gaagcgaagg
     5221 gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag aaattctgga
     5281 aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat tttattcacc
     5341 agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag gagatgaggt
     5401 aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg tagttactca
     5461 actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct atgtccgatt
     5521 gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat attcacactg
     5581 aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac gcaggttttg
     5641 agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat tatcaggaag
     5701 ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt tatacaccgg
     5761 aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt gagcagattc
     5821 aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg ctggataacg
     5881 cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc aacatcccgg
     5941 gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat gaaggtatgc
     6001 tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc actgttcacg
     6061 gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg atccgggaaa
     6121 cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg tcctctgaaa
     6181 tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc cgtatgtggc
     6241 gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag taactaccct
     6301 gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac atgcccccaa
     6361 tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt tcactcctgt
     6421 gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg agaatgccct
     6481 gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa acacttttgt
     6541 gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata ataacctgca
     6601 aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca tcgcctatcc
     6661 aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag tgatgagact
     6721 gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg caaattacga
     6781 gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg aaaccatttt
     6841 ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa caccgcgctg
     6901 gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt tggtggttgc
     6961 tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga gtggattgcc
     7021 accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc cggttgtgcc
     7081 tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga cgggtgaact
     7141 gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg atagcccaca
     7201 gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa catccctatg
     7261 ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc tttcgtgctg
     7321 gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc agagcggatg
     7381 agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg ttagaagatg
     7441 gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg gaacgtctgt
     7501 cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca tctcgcggcg
     7561 tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg acggtatccc
     7621 gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg ttgggtatgg
     7681 acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt ggcatcagtg
     7741 gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc tatgacatgt
     7801 tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc tcaaagcgta
     7861 atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt ctctggcacg
     7921 gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg tccgcgtgaa
     7981 tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat gtgtatgttt
     8041 tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga gtctggcgta
     8101 tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc atcgctggga
     8161 cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc aaggctgggt
     8221 ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg cggaatagcg
     8281 taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc taccgagata
     8341 cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac caatcatcac
     8401 atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca aaactcgatt
     8461 gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt cgccaatggt
     8521 taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca tactgccatc
     8581 ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa tcacaacgag
     8641 cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac tgctatcgtc
     8701 gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta aaacatcaga
     8761 catacttacc tcagcaataa atgatttact aatgactttg ggggcattat tggccttgtg
     8821 caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa aaagggtgaa
     8881 aaaacaacaa acagaaaaga agatcaaaaa aatacttgtg caaaatattg ggatccctat
     8941 aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg aacaacgaag
     9001 ag
//
LOCUS       DAJTYM010000132         8964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTYM010000132 DAJTYM010000000
VERSION     DAJTYM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            152..1687
                     /gene="gadC"
                     /locus_tag="OO575_003495"
     CDS             152..1687
                     /gene="gadC"
                     /locus_tag="OO575_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6666327.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1818..3137
                     /locus_tag="OO575_003496"
     CDS             1818..3137
                     /locus_tag="OO575_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6666328.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3435..3476
                     /locus_tag="OO575_003497"
     CDS             3435..3476
                     /locus_tag="OO575_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666329.1"
                     /translation="MVQLVDLARCVSF"
     gene            3514..4659
                     /gene="dosC"
                     /locus_tag="OO575_003498"
     CDS             3514..4659
                     /gene="dosC"
                     /locus_tag="OO575_003498"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6666330.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4584..5201
                     /locus_tag="OO575_003499"
                     /pseudo
     CDS             4584..5201
                     /locus_tag="OO575_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5459..5791
                     /gene="ddpX"
                     /locus_tag="OO575_003500"
                     /pseudo
     CDS             5459..5791
                     /gene="ddpX"
                     /locus_tag="OO575_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5805..6701
                     /locus_tag="OO575_003501"
     CDS             5805..6701
                     /locus_tag="OO575_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6666331.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6698..7672
                     /locus_tag="OO575_003502"
     CDS             6698..7672
                     /locus_tag="OO575_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6666332.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7665..8591
                     /locus_tag="OO575_003503"
                     /pseudo
     CDS             7665..8591
                     /locus_tag="OO575_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8647..>8964)
                     /gene="osmC"
                     /locus_tag="OO575_003504"
     CDS             complement(8647..>8964)
                     /gene="osmC"
                     /locus_tag="OO575_003504"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6666333.1"
                     /translation="RFEGEKGTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTA
                     DVSLDKVDAGFAITKIALKSEVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQ
                     LKS"
BASE COUNT         2180 a         2194 c         2261 g         2329 t
ORIGIN      
        1 gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca aagtctgttc
       61 actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa caggtgcggt
      121 tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga caggtaaagc
      181 taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa tggctgttta
      241 tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc tattaggcgg
      301 gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg tcgacggctg
      361 ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat ggggatttgc
      421 agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc tctatttcgt
      481 gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc ccattaccaa
      541 aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg gtggcacgaa
      601 atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac ctgcatttat
      661 tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta tcgaaatgga
      721 ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat ttgttgcctt
      781 cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa tgagcaaccc
      841 cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct gcttaagctc
      901 tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc tctccgcagg
      961 ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg agtggacggt
     1021 tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct cctggattgt
     1081 tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag cggcattcgc
     1141 taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg tgattacttc
     1201 tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct tcctgatcgc
     1261 actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta ttggctacat
     1321 tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg gtggtaaagg
     1381 ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt ttattgtttc
     1441 cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg aattactggt
     1501 tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc atgatcgtaa
     1561 aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg caccaaaagg
     1621 tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga atgacaagaa
     1681 acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg ctcccttttt
     1741 cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc acatctgttt
     1801 accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg attagaagac
     1861 cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg cctccagagt
     1921 ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa tcgtcacaac
     1981 cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg gtttcctcgg
     2041 ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg atcgacaaac
     2101 tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag ccggacggta
     2161 ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt aagattggtg
     2221 aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag cgtgggatga
     2281 aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc ggtactatca
     2341 gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa caccgtgact
     2401 ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag gttcaggact
     2461 ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc gtgcagtttg
     2521 acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa acgtaccgta
     2581 aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact cagcagttaa
     2641 ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt ggcgttagcc
     2701 cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg
     2761 cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga ttgctggatt
     2821 acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat gacgtgttgg
     2881 caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc ggtatcgcct
     2941 tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac ggcggcgtac
     3001 cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc ggcaccatct
     3061 tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc tatctgcaaa
     3121 gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct ttacccaccg
     3181 gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt caaattaacc
     3241 acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg tatgaatatg
     3301 aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat actcttctga
     3361 agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag tatatcttca
     3421 tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg ttttgattcc
     3481 gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa aagaatgaaa
     3541 gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa agccgcagaa
     3601 attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt ccgcatcgac
     3661 ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg
     3721 gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag gctaatacaa
     3781 atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga aattgtcgag
     3841 atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc ggattattcc
     3901 gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat cgcgatggaa
     3961 gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga tgaaaactat
     4021 cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat agcctcaata
     4081 ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt aggcagcagt
     4141 ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg acattatttt
     4201 agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga cggtattttc
     4261 aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt gaaatttcta
     4321 ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa
     4381 gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg ccgtttcctg
     4441 ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc gctgtcagtg
     4501 ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca taacactggc
     4561 gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa atcgaaaata
     4621 ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt agccagaata
     4681 ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt aatcagctgc
     4741 ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag ctgacggtgg
     4801 aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc attcagatcc
     4861 tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt tccggattat
     4921 cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt gtcgatcgtt
     4981 gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt gggcaaagcc
     5041 tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag atgctacgta
     5101 agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc gccgaagaaa
     5161 ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca
     5221 ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc agctacgatt
     5281 ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg ctgcacaaat
     5341 gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta tcccggcatg
     5401 gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg aaccgtttat
     5461 gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg agatcgaatt
     5521 gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag cgcgttgtct
     5581 gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg
     5641 cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga tgttgtggca
     5701 agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc acagccgtgg
     5761 cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg ctaagcgagg
     5821 aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa ctggtatgga
     5881 tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta atgcgactga
     5941 tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat ttaaccgccc
     6001 gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga cgcgatctgt
     6061 ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg gtggtcgcca
     6121 ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc ggacgcgcag
     6181 acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg gtactgacaa
     6241 tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg attgctattg
     6301 tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta cgccagtata
     6361 cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc aactggcata
     6421 ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc ggtagcgcga
     6481 ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg agtgctgaat
     6541 ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg tattgcgcat
     6601 ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt gatggtattc
     6661 gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg ttctggacat
     6721 tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc tcaacaatgt
     6781 gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg gctcaggtaa
     6841 atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt gcgtacatcg
     6901 ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac agcttcgtca
     6961 gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc ttaatccgac
     7021 acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa taagtcgtcg
     7081 ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg atgccgtgga
     7141 agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg taatgattgc
     7201 gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga cggcgctgga
     7261 cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg ccagtggaac
     7321 tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg atagcgttta
     7381 cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta tccatcatcc
     7441 ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag taccacgcca
     7501 gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg acggctgcgc
     7561 ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt gtggtgacaa
     7621 caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct gacacgttat
     7681 taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt ggcaaatcta
     7741 cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt gaaaccttag
     7801 ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta atgggcatgc
     7861 tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg cagatggtgt
     7921 ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc atcacagaat
     7981 cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca gaggagttgg
     8041 ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg
     8101 ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac gtgattgtgc
     8161 ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc aatttgctgg
     8221 taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat gtctcggtga
     8281 tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta gaactgggcg
     8341 acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg ctggattccc
     8401 tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg gatctgccgg
     8461 gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg gcaacccacg
     8521 gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc cggtgctggc
     8581 gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc gtcatccggc
     8641 aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta cctgagagac
     8701 cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat caatacccgg
     8761 caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat ccactttatc
     8821 cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt cccccagcat
     8881 taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt
     8941 ggttcctttt tcgccttcaa aacg
//
LOCUS       DAJTYM010000133         8889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTYM010000133 DAJTYM010000000
VERSION     DAJTYM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..700
                     /gene="hchA"
                     /locus_tag="OO575_003505"
     CDS             <1..700
                     /gene="hchA"
                     /locus_tag="OO575_003505"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6666334.1"
                     /translation="LVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVA
                     TISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPG
                     GHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPD
                     AADKQTPDIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANAL
                     GKLAAQEMLAAYAS"
     gene            complement(808..2166)
                     /gene="hprS"
                     /locus_tag="OO575_003506"
     CDS             complement(808..2166)
                     /gene="hprS"
                     /locus_tag="OO575_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6666335.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2166..2837)
                     /gene="hprR"
                     /locus_tag="OO575_003507"
     CDS             complement(2166..2837)
                     /gene="hprR"
                     /locus_tag="OO575_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6666336.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2970..3383
                     /gene="hiuH"
                     /locus_tag="OO575_003508"
     CDS             2970..3383
                     /gene="hiuH"
                     /locus_tag="OO575_003508"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6666337.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3492..4496
                     /gene="msrP"
                     /locus_tag="OO575_003509"
                     /pseudo
     CDS             3492..4496
                     /gene="msrP"
                     /locus_tag="OO575_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4497..5132
                     /gene="msrQ"
                     /locus_tag="OO575_003510"
     CDS             4497..5132
                     /gene="msrQ"
                     /locus_tag="OO575_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6666338.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5390..6040
                     /gene="zinT"
                     /locus_tag="OO575_003511"
     CDS             5390..6040
                     /gene="zinT"
                     /locus_tag="OO575_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6666339.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7118..7387)
                     /locus_tag="OO575_003512"
     CDS             complement(7118..7387)
                     /locus_tag="OO575_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6666340.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7444..8112
                     /locus_tag="OO575_003513"
     CDS             7444..8112
                     /locus_tag="OO575_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6666341.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8428..>8889
                     /locus_tag="OO575_003514"
     CDS             8428..>8889
                     /locus_tag="OO575_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6666342.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVL"
BASE COUNT         2533 a         2005 c         1823 g         2528 t
ORIGIN      
        1 tctggtgatc gccgcggacg aacgttattt gccgaccgat aacggaaaac tgttctcgac
       61 cggtaaccat ccgattgaaa cgttgctgcc gttgtatcat ctccatgctg caggtttcga
      121 attcgaagtg gcgaccattt ccggtctgat gaccaagttt gaatactggg ctatgccgca
      181 caaagatgaa aaagtaatgc cattctttga acagcataaa tcgttattcc gtaatccgaa
      241 gaaactcgcg gatgttgtcg ccagcctcaa cgctgatagc gaatatgcag caatctttgt
      301 tcctggtggg catggcgcac ttattggttt accagaaagc caggacgtgg ctgccgcttt
      361 acagtgggca atcaaaaatg accgttttgt tatctccctt tgccacggtc cggcggcttt
      421 tctggcactt cgccacagcg ataacccact gaatggttat tctatttgcg cattcccaga
      481 cgccgcagac aaacaaacgc cagatattgg ctatatgccg ggtcatctca cctggtactt
      541 cggcgaagag ttgaagaaaa tgggcatgaa catcattaat gacgacatca ccgggcgagt
      601 acataaggac cgtaaacgtc tcaccggcga cagtcctttt gcagcgaatg cgttgggtaa
      661 actggcggcg caggaaatgc tggcagctta cgcgagttaa tccctgttac attgtgatgt
      721 aagcgcataa tcaatcgcag ccacgttaat actcacgacg tggctgcgtt ttttcacggt
      781 taacttatgg cgtactgaag cccgatgtta atttctttgc ggtaacgtaa ttcggaacac
      841 gttatgctta ctgagatagt gatacgtagc acttcctcca tgtaattcgg caatcgcttt
      901 gactaaagaa aggccaagtc cctgacctac ggaatgacgc gaattatctc cccgccaaaa
      961 tctacggaag agtttttcag gctcattaat tttcgttcca ggactggcga tatcaatatt
     1021 aagagagccg ttggcatcaa gaaaactggt tatatgaata cgcgattttt ctggtgaata
     1081 tcgaatggcg ttaacaataa gattcgataa cattcgttgc agtaaaattt tatccgcaaa
     1141 gatttgttga ttgcacttga ccttaaagca aatctctttc tcgtctgaaa gatattcaag
     1201 atagtccaaa agattttcaa cttccttatt gagagaaagc gaatccagtt tcaccaaaac
     1261 attgtttttg tctgccctgg caagaaacag tatattctct gttaaccgcg aaatattttc
     1321 cagctcttca atgtttccgg caattgtttt ttgatattca gcgatacttc tgatttgact
     1381 gagcgtaacc tgattctgac ccagtaatgc attaattggc gttctaagtt catgagcgag
     1441 atcgtcagca aattgactaa ggcgctcaaa atctttgact aaagcatgat gcattttatt
     1501 caacgcctgc ccaagaggtt ttagttctct cggtaatgcg ttaacctcaa ccggcactcg
     1561 gctatcgtta taattcagcg cttccgttac gccactcaac tttttgatct ctcgcaaccc
     1621 cgtcctgatt aacaacggac ttaatactga gcacagtata atggcgacaa tgcaaattat
     1681 aatgctatta attttatact gttcaagcat attatgtctg gctgaagcca atttagccac
     1741 agtaaccgtt aatgatggcg aaacttcatc aatgttaatt cgtaaagcat ctatctctgt
     1801 atcattaata atgcttctgt aaatgcctgc tgcgctgatt gtctcactag caggtatgtt
     1861 atttaacatg tcatcactga catttgtccg gttaacaatt ttattgatgc catcactatg
     1921 aatgatcaaa atatcctgac taacatccat catccggtta aagtacacag gtaacgtatc
     1981 tggatttacc ccatcaatta acaactgctt gatctgcgct gtccggttaa tgagtgttgt
     2041 atcatcgcgc catttcaact cacttgccag gccattatag agagtccata caattccagc
     2101 gccagcaaca gactgcagaa atataaaaag caaggttaaa cggacggtta tagataatct
     2161 tttcattatt tttttaccgc tacgaatgaa tagcccatcc cccggattgt ggcgattagc
     2221 ttttcaggaa aaggatcatc aacttttgcg cggagcctgc gaatggcgac atccaccgta
     2281 ttggtatcac tatcaaagtt gattccccaa atttcactcg caataaccgt tctgggtata
     2341 atctcgccag ctctggaggc cagtagccaa agtaactgaa actccttgcg cgtcagtgta
     2401 atactgatat tgtccctgct gacactttga ctgacagagt ccatttttaa gccactgatt
     2461 tctaatgttg aattcagagc gtgatgttgc cttaattgtg cccgaaccct tgctagcaac
     2521 tcagaaaatg aaaaaggttt taccagataa tcatttgccc cactgtccaa ccctctgact
     2581 ctgtcatcga cagaatccct tgcagtaagg caaataacag gggtttgctt tgccgttctt
     2641 aacgtttgta agatctgcca gccatccata cccggaagca taatatccag aatgatcaat
     2701 gcataatcat ccttcagcgc aagataaagc ccatctctgc catcagaaac ggcatcaatg
     2761 acataacccg cttcggaaag cccctgcgtt acccattcct gggtcctttg attatcttca
     2821 ataagtagaa tcttcatgaa aataatatgc cataaatcat atgttataga aacaacctgg
     2881 ttcattacaa aattgtaatg ctgctgtaag gttaccctgg acgttttttc gctatcctca
     2941 aaactcattc acatgacaag gatataaaca tgttaaagcg ttatttagta ctctcggtag
     3001 caacggcagc cttttcatta ccttctttgg tttatgccgc acaacaaaac attcttagcg
     3061 tgcacatttt gaaccagcaa acaggaaaac ccgctgccga cgtgacagtc actcttgaaa
     3121 agaaagcgga taacggctgg ttacaactta ataccgccaa aacagataag gatggacgaa
     3181 ttaaggcact gtggcccgag caaactgcga ctactggcga ttaccgtgtc gtatttaaaa
     3241 ccggggacta tttcaagaaa caaaatcttg aaagtttctt ccctgagatt cccgttgaat
     3301 ttcatattaa taaagtgaac gagcattatc atgtgccttt gttactcagc caatatgggt
     3361 attcaaccta tcgtggcagt taatgcgtaa atatgtaaaa gctgatctct gctactgtta
     3421 gggaggtgac tccctgacag attaacagta aacagctctt gttggttaac gacaaaaaag
     3481 tgtgatggct tatgaaaaag aagcaatttt taaaagaatc agatgttacg gccgagtcgg
     3541 tattctttat gaagcgtcgg caggtgttaa aagcactggg catcagcgca gctgcatttt
     3601 ctttgcctca cgctgcgcat gccgatctgc ttagctggtt taaagggaac gatcgtccgc
     3661 ccgcccccgc cggaaaaccg ctggagttca gcaagcctgc cgcctagcaa aataacctgc
     3721 cactgacgcc agcagataaa gtctccggtt ataacaactt ctatgaattc gggctggata
     3781 aagacgatcc cgcggctaat gctggtagcc tgaaaaccga tccatggaca ctgaaaatca
     3841 gcggcgaagt ggcaaaacca ttgaccctcg atcatgatga tttaacccgt cgcttcccgc
     3901 tggaagagcg tatttatcgt atgcgctgcg tggaagcgtg gtcgatggtg gtgccgtgga
     3961 ttggttttcc gctacacaaa ttgctggcgc ttgccgaacc caccagcaat gcgaagtatg
     4021 tcgctttcga aacgatttat gcgccggaac agatgcctgg ccagcaggac cgctttatcg
     4081 gcggcgggct gaaatatcct tatgtcgaag gattgcgtct cgacgaagca atgcatccgc
     4141 tcacactgat gaccgtaggt gtttatggca aggcgttacc accgcaaaat ggcgcgccgg
     4201 tgcgactgat tgtgccgtgg aaatatggct ttaaagggat taaatcgatc gtcagtatta
     4261 agctgacccg cgagcgtccg ccaaccacct ggaatctggc agcgcctgac gaatacggtt
     4321 tttacgccaa cgttaatccg catgttgatc acccgcgctg gtcacaggct accgaacgat
     4381 ttattggttc aggcggcatt ctcgatgttc agcgccagcc aacactactg tttaatggtt
     4441 acgccgacca ggtggcatcg ctgtatcgtg gcctggattt gcgggagaat ttctaaatgc
     4501 gtctgacagc aaaacaagtg atatggctga aagtttgcct gcatcttgcc ggattgttgc
     4561 cgtttctctg gctggtctgg gcgatcaatc acggtggact gggtgccgat ccggtgaaag
     4621 atattcagca ttttaccggt cgcactgcac tgaaattttt gcttgcggcc ttgttaatca
     4681 cacctctggc acgctacgca aaacagccgt tattgatacg cactcgccgc ctgttaggct
     4741 tatggtgctt tgcctgggcg acactgcatt taaccagtta cgcattgctg gagttgggcg
     4801 tgaataatct ggcgttatta ggtaaggagt taattacccg gccttattta acgttaggaa
     4861 ttatcagctg ggttattttg cttgctttag cgttcacttc aacccagtcg atgcagcgaa
     4921 aactcggcaa acactggcaa cagttgcata acttcgtcta tcttgtcgcg atcctggccc
     4981 caatacatta tctgtggtca gtgaagatta tttcaccgca gccactcatc tacgccgggc
     5041 tggctgtact gcttttagcc ttacggtata agaagttgct ttcccttttc aaccggttac
     5101 gcaagcaggc ccacaacaaa ttatctctat aacttcatat tgccgacaaa cttcgcgtta
     5161 tcccggccgg atgcggcgcg agcgccctat ccggcccaca cagatcgcat caagttatgt
     5221 ttcgtttgtc atcaaccttt catcaccaga ataatttatt ttatttcaat aagatagttc
     5281 aatacacctg agaataccat gctctcattt cctaatgagt tgttgcattt tttcatatgt
     5341 tacaatataa cattacacat aatgtacatt aactctggag gaaattgttt tggcgattcg
     5401 tcttcataaa ctggctgttg ctttaggtgt ctttattgtt agcgctcctg ccttttcgca
     5461 tggtcatcat tctcatggca aacccttaac agaggtcgaa caaaaagctg ctaatggtgt
     5521 ttttgatgat accaatgtac aaaaccgaac gctcagtgac tgggatggag tctggcaatc
     5581 cgtttaccct ttactgcaaa gtggcaaact tgatcccgtc tttcagaaga aagcggatgc
     5641 agataaaacc aaaacatttg ctgaaattaa agattattat cgcaaaggtt atccgacaga
     5701 tatcgaaatg attggcattg aggacggcat tgtcgaattc catagaaata atgaaacaac
     5761 atcctgtaaa tatgattacg atggatacaa aatactcacc tataaatcag gcaagaaagg
     5821 cgttcgttac ttattcgaat gtaaagatcc tgaaagcaaa gcccctaaat atatacaatt
     5881 tagcgatcat ataattgcac cacgaaaatc atctcatttt cacattttta tgggtaatga
     5941 ttcccagcaa tctttattga atgaaatgga aaactggcca acgtattatc cctatcagtt
     6001 gagtagcgaa gaagtggtcg aggagatgat gtctcattga tttttttgcg ctgacttcag
     6061 ttatttaaca gaacagtgcc tggtagtaac aacaataatt taccaagaca aataagtaag
     6121 acaaactcag acacgctcat acttgagtcg tgtctttttt attatgtctg tagaaaaaaa
     6181 ctgagaaaaa ggctaacaat aaatacacaa ctcagccata cagactggca gcgcaaatgt
     6241 ttccaggagc aagtcatctg tcatggtaaa gctaaccaga aatataggtt aattttggtg
     6301 tttaaacctt ttttacttgt tagtatgata caaaggcttt caaaaaagct gggaaattca
     6361 acaactcttg gaaattatgg actttgtccc gcggctttaa tctgacggcc gcgttccttt
     6421 tttggctcaa ttattgaccc cttctctacg caacttcagt tcccgccagc aactttgcgg
     6481 cagtttcata ggatcaatgt ctaaaagaat aatggtgacc gataagaaaa cgactgaata
     6541 actgtagatt ttcgctcgaa accttcctgt cagctccata gcgaatcaag cgctgaatgt
     6601 cacagtatcg aacagaaaac agtgacgatc taacccttca agaatattct acgattgttc
     6661 tgtttaggaa aagcaaggcg ggaagtcggg agataagtca ttgataaaat ggcggagaga
     6721 gggggatttg aacccccggt agagttgccc ctactccggt tttcgagacc gatgatacag
     6781 agtcgaaaaa tcaaagagtt aaaaattttt cttggaaaaa atgtcttaaa atcgtacacg
     6841 aaaaatcaat gaattacata acattaaaga agaatattcc aagccttctt gagtccacca
     6901 cctgttttca ggtgtcattc agacgtgatt tgccccagat tgggaaagca cgcttttttc
     6961 tcagcttatt ccgttttgcc tgtgtgttga ataattccac tgcagttata taaaatcggt
     7021 aacggctgga aatcattcaa tactcgcact atcgaaagtt taccagccaa ccgcagcacg
     7081 tcctgcatac gtcgtgtctg cggtttttct ttttcgctta cattgtgtct ggttcttccg
     7141 gccactcaat atcaggtgca gttgatgtat caacacggtt cagcaacacc cgatacttct
     7201 tccaggcttc cagcaacgag ttttcttcct ccgttgcgat ctccagatct acagcatcct
     7261 gaagtggcgc aatatgctca ctgaatccct ggatgtagaa ctgtgtggtg acggtcttcc
     7321 agccattcgg ctcctgctgt atcgaagcat accaggctat ttcaatatcg ctatgctgcg
     7381 gcagcattta accccttgta attcatcgcc ataattgatt taattcacaa ataaaactat
     7441 aatatggtga aattaatgaa aaaaaacaca gatgatgggg ctaaaattta cacaccactt
     7501 accctaaagc tttatgactg gtgggttttg ggagtatcaa atcggcttgc atggggatgt
     7561 cctacaaagg aacaccttct tccacacttt ctggaacatt taggtaacaa ccatctggat
     7621 attggtgttg gaactgggtt ttaccttact cacgtacctg agagtagtct gatatcttta
     7681 atggatttga acgaagctag cctgaacgcg gcatctacaa gggctgggga atcaaaaatt
     7741 aaacataaaa ttagccatga tgtttttgaa ccttatcctg cggcgttaca tggtcaattt
     7801 gattccattt ccatgtttta ccttcttcac tgcctgcctg gaaatatatc tacaaaaagc
     7861 tgtgtaatac gcaatgcggc gcaggcctta actgatgatg gaactctata cggagccaca
     7921 attcttggcg atggagttgt gcacaatagc ttcggtcaaa aactgatgcg catttacaat
     7981 cagaaaggca tcttttcaaa cacaaaagat tccgaagaag gcttaacaca tatactctca
     8041 gagcatttcg agaatgttaa aaccaaggtt caaggtactg tagtaatgtt ttccgcttca
     8101 gggaaaaaat agcatccaac cgcagcacgt tcttgcttaa gacgtgctac ggtttccttt
     8161 acgatttggg gttgcgactt tacctctatt gataatgcat tccggcagaa cgttcaaata
     8221 tgaacgtacg atatttaact aaccgaaaaa caaaatatag cacaggcgag acatttacca
     8281 tctaagaaaa tagtatcgtt tttttacagt taaatcaaca tcacttcctt aaaatgaaaa
     8341 caataaattt caatcctgaa acattttatt tcagcttatt attccccagt atcacatttt
     8401 atcaggatat ccttctgcga ggttattatg cttcctgtaa ataatccccc cctatccact
     8461 ggaaacgtct ctttttacag aactacatca atcgacaatg ttcacaataa ttatctctcc
     8521 gaatgggttg aatggactaa aaacagcatt tccggagaaa acagggaaac tgcttttacc
     8581 cggctccaat tatgtctgga gaacagtgaa acatcgttgg acttatcttg tttaggtctc
     8641 agatctctac cacgattgcc tgacaatctt gatgaaatta atgtaagcaa taaccaacta
     8701 tcaatgctcc ccgagctacc aagggcattg aaagagctga atgcaagcag taatcaatta
     8761 tctgcacttc ctgaattacc agtgtcgctg gaatatataa atgtgagtga taaccatttg
     8821 ttcgcacttc ctgaattacc tgcgtcacta gaatatatta atgtaagtga caatcacctg
     8881 tctgtactt
//
LOCUS       DAJTYM010000134         8865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTYM010000134 DAJTYM010000000
VERSION     DAJTYM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..690)
                     /locus_tag="OO575_003515"
     CDS             complement(<1..690)
                     /locus_tag="OO575_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6666343.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQ"
     gene            750..1028
                     /locus_tag="OO575_003516"
     CDS             750..1028
                     /locus_tag="OO575_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666344.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1029..1556)
                     /gene="gadE"
                     /locus_tag="OO575_003517"
     CDS             complement(1029..1556)
                     /gene="gadE"
                     /locus_tag="OO575_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6666345.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2355..2927)
                     /gene="hdeD"
                     /locus_tag="OO575_003518"
     CDS             complement(2355..2927)
                     /gene="hdeD"
                     /locus_tag="OO575_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6666346.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3182..3514
                     /gene="hdeA"
                     /locus_tag="OO575_003519"
     CDS             3182..3514
                     /gene="hdeA"
                     /locus_tag="OO575_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6666347.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3630..3956
                     /gene="hdeB"
                     /locus_tag="OO575_003520"
     CDS             3630..3956
                     /gene="hdeB"
                     /locus_tag="OO575_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6666348.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4020..4666
                     /locus_tag="OO575_003521"
                     /pseudo
     CDS             4020..4666
                     /locus_tag="OO575_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4708..5238)
                     /locus_tag="OO575_003522"
     CDS             complement(4708..5238)
                     /locus_tag="OO575_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6666349.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5394..5960)
                     /gene="slp"
                     /locus_tag="OO575_003523"
     CDS             complement(5394..5960)
                     /gene="slp"
                     /locus_tag="OO575_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6666350.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6207..7430)
                     /locus_tag="OO575_003524"
     CDS             complement(6207..7430)
                     /locus_tag="OO575_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666351.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8011..>8865)
                     /locus_tag="OO575_003525"
     CDS             complement(8011..>8865)
                     /locus_tag="OO575_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6666352.1"
                     /translation="GARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLT
                     FLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSS
                     SANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDAL
                     QQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAV
                     IPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILM
                     GYLTQLLF"
BASE COUNT         2603 a         1740 c         1846 g         2676 t
ORIGIN      
        1 ttgcccactg gcgacctctt ctttcatgcg taagaaatct tgcaccgact gcgtgagatc
       61 gacataaatc gggtccagac gttgtacggt aaccagcgaa tctgcctgat tagcggtaac
      121 gagtgcgccg acggtcaccg acgatttccc gctgacgccc gtaatcggcg aggtgacatt
      181 cgcgtattgc agattgatcg tcgcctgttc aacagccgct ttggcgacgg tgacattggc
      241 ttctgcttca ttcagctggg tgcgcgcggt gtcgtaatcc tgacggctaa cgtagttggt
      301 cttcagcaac gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg tagagagcgc
      361 tttcgccagc gagcctttgg cggagtttag ctcggcctgt aaaggtgcag gatcaatctg
      421 atacagcgaa tcgccctggt tcactttatc gccttcgata aagttgcgtt taatgataat
      481 accgcccacc tggggacgta tctcggcaac ttcataagga acggttctac cgggcaattc
      541 gctcaacaca ttgaccgaac caggggagag tgtgacgaca ccgacctcag gcgtcatggc
      601 ggcggcgttt tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc agaataacaa
      661 cggtattaac agctttcttc ttctgttcat tttagtccct gaaaattctt gagaaataac
      721 ggttctttaa attgcgcgcg aggagagata tgagggattt acagaccagc ggcatagtgg
      781 ggttgtcagc cagtaaggtg ggatacaggc acagtgatcg acatggtgag gtcaacgaca
      841 aaaaaaatgt tgccgttctg ccaacagttc ctgccagcat tcgggcaacg aagggttcta
      901 ctggtggata cacataccag gggaatcaag tcgttttagc ctctccttta gtaatcaccg
      961 ggggtaatga aatcagcatc tgcatccctc gtcatgccag ccatcaattt cagttgctta
     1021 tgtcctgact aaaaataaga tgtgataccc agggtgacga tgtcgctcat acgtcttagt
     1081 ttcagcttgt acataatgtt gaatttctga atctttaatg tttgttgcga aatattgagc
     1141 gtcgacgtga tattgctttc atttttattt ttcagaatca gttgcataat tttgaactga
     1201 ttatgtgaaa aacataattg tgaattctta tggggcaagt gtttaccata agtaatctca
     1261 agaagatttt tgatactttc tttgcggcta acaaaataaa taggcgcttt agcttttagt
     1321 ttactgatgt gataaggtgc caaaacaata attctctcgg catctaattt ctccagaaat
     1381 ttaatcgctt cttcatcaag gatatgattg tgataggtat caatgataac cttgaagggt
     1441 ttattgccta cttttttgat ctctgacagg gaattgattt ttatcatttc gtgattatct
     1501 ttcaactgcc aaaagccctg taaaagaaaa gaatctttcg tcatgagaaa aatcataact
     1561 tgctccttag ccgttatcgt ttcgaacatg tcatcctttt cattatttac atccttgtcc
     1621 gaatcgttgt tcaatatagt aaacgcctat aagaactttg cccttcgtga actaatacgt
     1681 ctattaacac taatcaatac ttcagattta tccatcacgc actccatata ttgatcctaa
     1741 gtaagattcc tggttgttat cagcttgtaa atagtaaaca aaaagttaac gtttgcggat
     1801 tactgcccaa gaataagtta tcaataacca gtttgtgatc tctgaagaat attactaaag
     1861 ttaaaatgta atccgattta aatatcgagt ctccttgttt cgacttaagc tggcaattgg
     1921 attgccagct ttcttttaac gcattgaaat tacctgagta aataacgcat tcatgtcttc
     1981 aggtttttat atagcgtctg gagacacgga tatttatgca atgtaatcaa cgccagttgc
     2041 aaaaaaatga acccggatta cttatttaac cgcccatagc atcaacgctt acggaactct
     2101 ttgttcactt tcccacagca cttttaaaac attaaacctg actacggaaa atatcagcca
     2161 tgataatgtt tgaaatggct aatttgccat ggagtgaaaa aaattagatg aaattcagta
     2221 ggttgaaata atcactagca ggtaattatt tcaatgatag tgcgcaattg atctacaaca
     2281 ctgcgtagcg gagagagtat taatcggatc atagtcacat caagtgacta tgatccgggt
     2341 gacaaccggg gtaattattg ctgcttaacg aacaaactgg cgaagctgaa caggctggcg
     2401 gcgctaaata tcagttcaat tcccaccagt gtggaaacca gcgttacaga caccatcggc
     2461 gttgcaccaa ggaatatcca ggcaatgacg atatccagca caccaataac gagctgtagc
     2521 cagctgcctt tcattgaacg ctgacgatac caactcatca ggcgaataac ccctgcaaca
     2581 cagaacaaac cggcaataaa tgccgcaatg gcaaaaatgc ccagctccgg tgcacggatg
     2641 aagaaatagc cgatcaataa ataggcgact gcgacgagga aaccggataa taccggccag
     2701 aaattatgac tgcggttgct gaataacccg acaataagcg caatacccga gcagattaat
     2761 aatgcaccca ctactgtgct taaaatatcg ccagagacga acgggaaact gatacacagc
     2821 aacccgacga taaacagcag cacggcaata aactggattg ctctgcgatg ctttttcagc
     2881 atctccagat caaacttcaa aattgttgcc ttatctatat ataacataga accaccctat
     2941 aaaattaaga agaaaatccc ctgctatcaa tctatgccaa aaacgcgtct aagaatgcag
     3001 tcgatttaat aaaaatttcc taattgcagt atctgatgca tctgtaactc attgtattga
     3061 aataaaaata tctgattttg atattttcca tcaacatgac atatacagaa aaccaggtta
     3121 taacctcagt gtcgaaattg attcgtgacg gctctttcac tttatagttg aggatattac
     3181 gatgaaaaaa gtattaggcg ttattcttgg tggtctgctt cttctgccag ttgtgagcaa
     3241 tgcagcggat gcgcaaaaag cagctgataa caaaaaaccg gtcaactcat ggacctgtga
     3301 agatttcctg gctgtggacg aatccttcca gccaactgca gttggttttg ctgaagcgct
     3361 gaacaacaaa gataaaccag aagatgcggt tttagatgtt cagggtattg caaccgtaac
     3421 cccagctatc gttcaggctt gtactcagga taaacaagcc aactttaaag ataaagttaa
     3481 aggcgaatgg gacaaaatta agaaagatat gtaattccgg gaatgcgtta catcgtactt
     3541 ccttgcatat tgaacaggcc ggaatatctt ctttaaaagc agctattcct cctgttcata
     3601 tataatctct atattgaatg ggttacaaaa tgaatatttc atctctccgt aaagcgttta
     3661 tttttatggg cgctgtagcg gctttgtcac tggtgaacgc acaatctgcg ttggcagcca
     3721 atgaatctgc taaagatatg acctgccagg aatttattga tctgaatcca aaagcaatga
     3781 ccccggttgc atggtggatg ctgcatgaag aaacagtata taaaggtggc gataccgtta
     3841 ctttaaatga aaccgatctc actcaaattc ctaaagtgat cgaatactgt aagaaaaacc
     3901 cgcagaaaaa tttgtatacc ttcaaaaatc aagcatctaa tgacttgccg aattaatgag
     3961 gtacaagtaa aaaggagtag caagttgagc catcttgctg ctcctttttg catttttata
     4021 tgacagcaga atttattata cgtcttatac tcgcggcaat tgcctgtggc gctattggca
     4081 tggaaaggca aatgcgcggc aaaggagcag ggttacgcac acatgtatta attggcatgg
     4141 gaagcgccct gtttatgatt gtttcgaaat atggttttgc tgacgtgctg tctttagatc
     4201 acgtcggact cgaccccagc cgtattgctg ctcaggtggt gacgggcgtc gggtttatcg
     4261 gtgcaggtaa cattctcgtt cgtaaccaaa atattgtagg tctgacgacg gcagcggata
     4321 tctgggtgac cgccgcatag gtatggttat tggcagcggt atgtacgaac tgggtattta
     4381 tggttcagtg atgaccttgt tggtgctgga agtcttccat caattaacct tccgcctgat
     4441 gaacaaaaat taccatttac agctgacatt agtgaacggt aatacggtat caatgctcga
     4501 ctggtttaag cagcaaaaaa taaaaactga tttagtttcg ttgcaggaaa atgaagatca
     4561 tgaagtggtc gccattgata tccagcttca tgccacaacg ttgatagaag acctgttgcg
     4621 attattaaaa ggtatggcag gggtgaaagg cgtttcaatt agctaatctg tgtggcagcg
     4681 tagccagact caccgtaagc ctgaaattca caccagataa tcaatatgct gatggcgtac
     4741 cagctcattg atccgcttaa gattgaggat catcatgata ttgtgtttat ggcagtagaa
     4801 cgtttttagc gatattttca gtttctccag aatatcttca gttgtcattt gattcgcaat
     4861 gcagctcaga acctgatgtt gcttgcgcga aagtgacaca ttcgcttcag gcacagagca
     4921 gtaactgcca ttgatgagtg cgacaaaatc aatgatagtg atagtccgcg gaacaaaggt
     4981 caccggtact ttccccagac agcgtttaat attaaagggg gagaaaacaa tgacatggac
     5041 aggttttaat cgttcaattt gagtgagaaa attagaatga aatacattat tcattaatgt
     5101 atcaataatg acgaaggcat tctgatgcaa cattacgtcg atctcttctt cgttctgtat
     5161 atgaacgaca ttacctttac tcagaatgtt tttcatcgcg gtgaagaaca tcgtatccct
     5221 ggtaattata agaaacataa ccgactcctg gtgcggacat taactttata atcagtttgc
     5281 caaaggtaat taacaggaca gggcttataa taataatagt tagcttactg tatattaatt
     5341 ttatgtaata catgatgcag atgctgagaa agcatctgca tctttcggtg gtgttatttg
     5401 accagctcag gtgttacctg actcaccgca ttggtgtagt aaggcgcacc ccagcccggt
     5461 tccggccaga atgcaccata gccgtaatcc cacaggttat aggtggtatt aaccacttct
     5521 ctcaaatgcc acacctggat gccctgcata ttcacttcca ggaagttata cgggacttta
     5581 ttgataaagc ctggttgttc accctgaatg gtaccgagga tggtaacaaa gtgattacga
     5641 tagttcactg gatcaaggaa gccgctttgt ctggcgagca gtcggccctg atagttggct
     5701 tcaatatcag gcttcgcata gctatccaac ggtaatacag cgatttctaa caacgtatcc
     5761 gttttgccat tgataacgtt gataaccttc ccaccaaagc gcgcttgttg accaacatat
     5821 aaccccggct ggttatgaac agcaacaaaa cttttttgaa tatcaggttg gttattgcct
     5881 ttgatatttt gcggaattga actacatgcg gcaagcaaaa atgaaaggct gaggatgagt
     5941 gcaccttttg tcatgttcat gttactatcc ttatcaacaa atattaaaac cataatagat
     6001 atttctttat agttttcatc tgattctgag gtgatatgcc tgatagtaac gtaaacctgt
     6061 ctgatgttta tatcccttct gtttagttca attccgttat tttggggcgt ggtatgagta
     6121 tccatcgccc aataatataa ccctttgttt taaaattaca taaaatctat ttttattcaa
     6181 tgattataaa gtgctaataa tcaatattat ggtattatat tctgataatg atttgataag
     6241 cgaattcttt tgccagatgg tatttctatt tccaccccat aatttatatc tttttcatta
     6301 ggcagatctc tctttaaata aaaggggtat gaaatctttt cgggttgaag tgtctcgtaa
     6361 tgaaattcta tgaattcatt tttagacatt gttgcccatt tttgaatttt attgtataga
     6421 gagggtgttg taaactcttg aagttgttta taacatattt ttttgaactc ttgcttatat
     6481 tttaatatgt catcaaatga aagattcact acctttgcct ctaagaaatc aattattctc
     6541 gaagcaattt ccgctttcgc gcgtaaagag ttgtcattaa tagttaacaa tgtagaaaac
     6601 aaaccattga tttgtttgtc aagatgttgt tcatatttat gtagtttggg gatgggattt
     6661 tccttgtgtg aaaaaatagt gttaaataaa tttgcgtcag tgaaatttat attaagtaga
     6721 tcttcaaaat aaaattttga taaggttagg actagcttgg acatatcttg ccctgtcatg
     6781 ttgaaatcaa taaagtcgca tccagcaagg gcttccatcc tattttcatt tttactgtgc
     6841 tcaatataca gcttcgccat ttttaagtac aagctttgaa tgtctcttat atcagttggt
     6901 aagtcagctc gttgcccagc aaaattttta ttatcggaag aaacggtttg gacactgaat
     6961 ctttcgggta ttccttcttc tacgagaatt ttagttatga ttggattgtt gggttctgga
     7021 ttaacgacaa caatgatttg acatacagaa tccttatcct tttcgtatct aatgttatga
     7081 atctgattaa gtattgattg tgtctgttgc gtaagtggtg tcgaacttat cgtttcaatg
     7141 ttattaataa gacttacaag ttgaaccaca acttttgagt agtctgcatt tgtatatgtg
     7201 gtgttcgtgt ggcatgcatt aatttgttgg agttgttgtt gcgctaaatc aaattgttca
     7261 gggttgtatc cttctccaga gaatgtggaa ttaatgggtt gttgttcttc ccaattatca
     7321 caaggccact tcggtgtgtc tggcgtttta acattatcgg tatttgttgt gcagttataa
     7381 atatcgccgt gatatgtatt tattatacct ggggtaaagt caattgacat aacattcctt
     7441 attcatcatc tgttttatgc taatgttttt aaattggata attagaacag gaatggattt
     7501 tcttctacac acaataaata taatgcgtta tatcttcctg atataattac ctttaaattc
     7561 cctttcattt ccacccggta atatttgtgt ataaaaaaag ggcagtgata gtatcgtaaa
     7621 ggaaaacgtt aacaactatc aaaaaacgct cattttaaaa agttattatg ttctccgata
     7681 gtgtgtgaag taatattttt gtgatgtttt agatgtgtaa taatgaacca ggttaaagtg
     7741 tatttaaaaa tgtgttgtat tagcgactga aatctgcctt ttaaaaagga ggagggtact
     7801 gagacaaaga gtcattgctt ctccgataaa atgtcagaga agcatgatta cgtggttatt
     7861 gtctgttgat gacttgttga ccgagccgtc atcattctca atgcattagt taaatcattt
     7921 gtgattttta aggattatcc tgtctatgat tttctactta gaaggatatg gcactaacta
     7981 tgcatcttat agtttactgc tggcatctat ttaaaatagc aattgagtca aatagcccat
     8041 caagatagcc atacctaata taaccgtcag gaatgcagct atcatcggca ttctgaacat
     8101 tgatttaagt aatatcactt ccgtcaggct tgcgccggca ctgccgatga ttaatgccat
     8161 taatgctccc agacccattc cttttgtcat cagaacagat gccagaggga taaccgcctc
     8221 agcccggata tacagcggaa taccgacaac ggcgcttaat gggatggcga agggattatc
     8281 tgctcctgca tgagcggcaa tccacgctga gggaataaag ccataaataa aagatcctat
     8341 taatactcct aacaaaagat atggcagtac atctttaaac tgttgcaacg catctttggt
     8401 ggctagtttt atgggattaa tacttactac agctttggta ctgcaacagg taactacggg
     8461 gttctcaatg gctttagccg ctggactaca acaactcact tttatgggcg tatatgtcgt
     8521 tcccggcgaa gtttttgctg gagcacaaca atttgctgat gagcttttac tggcaatgat
     8581 atgacgttca aaccccagag aatccaggat aatactggca agaacggaga cgccggctgc
     8641 gataatcgcg tacaacaagg tgactttcca accaaaggtc atccacatta gtccgacgat
     8701 aatgggatta agtaatggtg aaacaaataa aaaagtgaga gtcggaccaa acccggcttt
     8761 cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg gggtaacggc
     8821 tcccaacaga gcagccagga gatagcctct cccttttctg gcccc
//
LOCUS       DAJTYM010000135         8748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTYM010000135 DAJTYM010000000
VERSION     DAJTYM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            299..994
                     /gene="minC"
                     /locus_tag="OO575_003526"
     CDS             299..994
                     /gene="minC"
                     /locus_tag="OO575_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6666353.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1018..1830
                     /gene="minD"
                     /locus_tag="OO575_003527"
     CDS             1018..1830
                     /gene="minD"
                     /locus_tag="OO575_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6666354.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1834..2100
                     /gene="minE"
                     /locus_tag="OO575_003528"
     CDS             1834..2100
                     /gene="minE"
                     /locus_tag="OO575_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6666355.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2472..2816)
                     /locus_tag="OO575_003529"
                     /pseudo
     CDS             complement(2472..2816)
                     /locus_tag="OO575_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2851..2970)
                     /locus_tag="OO575_003530"
     CDS             complement(2851..2970)
                     /locus_tag="OO575_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6666356.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2931..3116)
                     /locus_tag="OO575_003531"
     CDS             complement(2931..3116)
                     /locus_tag="OO575_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666357.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3217..3390
                     /locus_tag="OO575_003532"
     CDS             3217..3390
                     /locus_tag="OO575_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666358.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3392..3736
                     /locus_tag="OO575_003533"
     CDS             3392..3736
                     /locus_tag="OO575_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6666359.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3740..4075
                     /locus_tag="OO575_003534"
     CDS             3740..4075
                     /locus_tag="OO575_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6666360.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4132..6453)
                     /locus_tag="OO575_003535"
     CDS             complement(4132..6453)
                     /locus_tag="OO575_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6666361.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6570..6779)
                     /locus_tag="OO575_003536"
     CDS             complement(6570..6779)
                     /locus_tag="OO575_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666362.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7530..>8748)
                     /locus_tag="OO575_003537"
     CDS             complement(7530..>8748)
                     /locus_tag="OO575_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6666363.1"
                     /translation="GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRIL
                     VTISDQHIRGALNVPLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTI
                     IFNPPPLFSFYRLIDKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEI
                     VPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQV
                     ARQMNAIASKLPEGFHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREP
                     LNVDESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADP
                     ESTRILDCVLDLARKLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVK
                     IILSKPKVKVVVE"
BASE COUNT         2461 a         2034 c         1906 g         2347 t
ORIGIN      
        1 gaaaaaatac acttgcctca atactggata tttaaacagg tctggagatg ctgtcacgat
       61 tagggtgcgg aaaaaggaag cgtaaataag aagaatcaaa agtacgacgg caatgggttg
      121 attgacaagg gtatttttta agctatgaat cagcgccatt tatcacagaa tagactttta
      181 ctctgaataa atgggagggt gacttgcctc aatataatcc agactataac atgccttata
      241 gtcttcggaa catcatcgcg cgctggcgat gattaatagc taattgagta aggccaggat
      301 gtcaaacact ccaatcgagc ttaaaggcag tagcttcact ttatctgtgg ttcatctgca
      361 tgaggcagaa cctaaggtta tccatcaggc gctgaaagac aaaatcgctc aggcccccac
      421 atttttaaaa catgcccccg ttgtactcaa cgtcagtgca ctggaagacc cggtaaactg
      481 gtcagcgatg cataaggcgg tttcggcaac cggtttgcgg gttattggcg taagtggctg
      541 caaagatgcg caacttaaag ccgaaattga aaaaatgggg ctgcctatcc tgacggaagg
      601 aaaagaaaaa gcgccacgtc cagctcccgc accgcaggct ccagcgcaaa atacaatgcc
      661 ggtcacaaaa acgcgtttaa tagatacccc ggtgcgttcc ggtcagcgta tttatgctcc
      721 acaatgtgat ctgattgtta caagccacgt tagcgctggg gccgaattga ttgccgatgg
      781 gaacattcat gtctatggca tgatgcgcgg tcgtgcgctg gcaggggcaa gtggtgaccg
      841 ggaaacgcaa atattttgta cgaacctgat ggcggaattg gtgtccatcg caggtgaata
      901 ctggctgagt gatcaaatcc cagcagaatt ttatggcaaa gcggcgcgac tgcagttagt
      961 cgaaaacgct ttgaccgttc aaccgttaaa ttgatccctt tttaacaagg aatttctatg
     1021 gcacgcatta ttgttgttac ttcgggtaaa gggggtgttg gtaagacaac ctccagcgcg
     1081 gccatcgcca ctggtttggc ccagaaggga aagaaaactg tcgtgataga ttttgatatc
     1141 ggcctgcgta atctcgacct gattatgggt tgcgaacgcc gggtcgttta cgatttcgtc
     1201 aacgtcattc agggcgatgc aacgctaaat caggcgttaa ttaaagataa gcgtactgaa
     1261 aatctctata ttcttccggc atcgcaaaca cgcgataaag atgccctcac ccgtgaaggg
     1321 gtcgccaaag ttcttgatga tctgaaagcg atggattttg aatttatcgt ttgtgactcc
     1381 ccggcaggga ttgaaaccgg tgcgttaatg gcactctatt ttgcagacga agccattatt
     1441 accaccaacc cggaagtctc ctcagtacgc gactctgacc gtattttagg cattctggcg
     1501 tcaaaatcac gccgcgcaga aaatggcgaa gagcctatta aagagcacct gctgttaacg
     1561 cgctataacc caggccgcgt aagcagaggt gacatgctga gcatggaaga tgtgctggag
     1621 atcctgcgca tcaaactcgt cggcgtgatc ccagaggatc aatcagtatt gcgcgcctct
     1681 aaccagggtg aaccggtcat tctcgacatt aacgccgatg cgggtaaagc ctacgcagat
     1741 accgtagaac gtctgttggg agaagaacgt cctttccgct tcattgaaga agagaagaaa
     1801 ggcttcctca aacgcttgtt cggaggataa gttatggcat tactcgattt ctttctctcg
     1861 cggaagaaga acacagccaa cattgcaaaa gaacggctgc agattattgt tgctgaacgc
     1921 cgtcgcagcg atgcagaacc gcattatctg ccgcagttgc gtaaagatat tcttgaggtc
     1981 atttgtaaat acgtacaaat tgatcctgag atggtaaccg tacagcttga gcaaaaagat
     2041 ggcgatattt ctattcttga gctgaacgtg accttaccgg aagcagaaga gctgaaataa
     2101 gcccgctgta aaaacgcatt tatcttcaag gcagagtcat ttctgccttg agtttttcat
     2161 ccctctcatc cacgttgtgg taaagcggcg agtattcttg ctgatactcc tcattgctat
     2221 ttccacctcc ccctttacac cttaaggctg ttcccacgca cagaacaaaa tgtttatgcc
     2281 tggttgagta aataaccatg ttattagtgt gtatatactg ggagatgata accccgcaat
     2341 ttcattattc attgttctcc cataggatga ggtttcccgc cgtaactggc gggctttttt
     2401 tgccccagaa ttttctcttt caacatcctg taaacgaaaa ctgcgccgaa gcgcagttta
     2461 atcgttgatg gtcagaacga ccagttcaca ccagccaccg cgttccacgg ggactccaca
     2521 ccggcaccat ggctataccc caccccgaaa tgcccgctta acgtactgct gaatgaggct
     2581 ttaatacctg cctggtatat tccacgtctg cccgacaaat cattgacaaa attaccgtca
     2641 ctattcactt tcacccgatt atcatcgaca aattctttgc gcacagccgc cttcaggcag
     2701 ggttcaattt ccataccgtt ccccagacgc atgttgtaac tcagcgttgc acccagttca
     2761 cgatatatac tgcgggtatc gactgatttc gattccatgc cattggataa atgatagtag
     2821 cgtctaagtg acctaagctc tgctgcaaca tcagcccgca ggcaccagac caggggattc
     2881 atcctgaaga gacagcgcag gtgtattgtg ttcaccgctc accaaagaca tcatgatgaa
     2941 atgatgatat cccgcataag aaagaggcat tttttacacg cagtgcgctg aagtgtagtt
     3001 ggataaaaaa tcactctttt cagataatac tgtcgtacgc ttatctttag acaatgaggc
     3061 cgtcggaaaa ataaaatgac taatgtaatc gttgctgatg aatatcttct gctcacataa
     3121 aaatcacaca ataatattga taaatcagat tatcttacct ttacaacatt gcgttaacag
     3181 tcaatgacac ttcttgaata aaaatattgt caatcaatga gttattcctc atttaagatt
     3241 attctcattc acgtaaaaaa cataattcct atcatcacag ccacgctcat tttgaattat
     3301 ctgaataata gcgagcgttc tttagttaag cagatcttaa ttgaagacga aattattgtg
     3361 tgtgcaactt acttaatacc ggacatctaa gttgaagaaa aaaatacttg ccttcgggct
     3421 tatctcagca ttgttctgct ccacgccagc gatagcggat atgaaccgca ccacgaaagg
     3481 tgcattgcta ggcgcaggcg ttggtttact tactggcaat ggtgttaatg gcgtactgaa
     3541 aggtgcagct gttggcgctg gtgttagtgc agtaacagaa aaaggccgcg acggtaaaaa
     3601 tgcgcgtaaa ggtgcaaaag tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg
     3661 caatggcctc gaaggggcga ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct
     3721 gggtaaaatg aaataaggaa tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt
     3781 tttcgcacca atgacctggg cgcaggacta caatattaaa aacggtttgc cgtcagaaac
     3841 ctatattact tgcgcagaag ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga
     3901 aattgttgcg gtaatgggta atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc
     3961 tccggaactg agcgccaaag tggtagaaaa actgaatcag gtttgcgcta aagacccgca
     4021 aatgctgttg atcacggcaa ttgatgacac catgcgtgct atcggcaaaa aataatattt
     4081 catatgtacc gcgtcgatga atacgacgcg gttttttagc taacggcggg atcagaactt
     4141 atatttcagg cccaccatca ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc
     4201 cacattcccc cagacgctgg ccgctggatt caggttgcca tttacgccaa gtttaatttc
     4261 ccccaaatta ctcaccccat cttgttcgac tttcacaccg ttcattgaga cagcaaaatc
     4321 tttactgtta tggatccagt tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc
     4381 gctgtttttc tcctgagtac gaataaaggt gcggaagccg agtcgggtct ggatgttgtt
     4441 atttgcgtca ttctcaatac gtgagccatt ggcttccgtg tgcgcattat gatcgacgcc
     4501 catccagata gcctgtgcct gtggctggaa gatccacgta tatttcgccg cttccagacc
     4561 aaaggattta ttcagggcaa aaccgtaacc cgcttccagc gagacggttg cacctttcgc
     4621 gctataggat tcataggcta attcatcccc tttcacggag tttttaaacc agctatattg
     4681 cgcccaggag tcaatgtatg cgcctttttt actgatgtca tcggcaaacc aagtggcata
     4741 cagccctgcg ctatagcctc tgacactccc tttcgaacga taatccgaca cgctggaatg
     4801 agttaagttg tagtcgcggg cataaccagc catcactccc aaacgccaac tgtcactatc
     4861 ggtaaaacca ccggttaaca gatcgccccc taactgcgag acgtagcgat gcgaggtcgt
     4921 tctcaactgt ccgttgctgt cacgccaggc attatgcccg ccaatttgac gtagccaaag
     4981 tcggcttgaa cgctcctgtt cagttacagg atcgatgtag cgcgtttcac ccgcacggtc
     5041 gttcagacgc ataacaaaaa gagtgttagc ggctgccagg ttggcgacgt aacttccagc
     5101 ttcggggcgc aggacggggg taggcgcaac agtaggcgct gaacttcctc cggagtttgt
     5161 ttcaccgccg ctcgttgcct ggaagttagt cagataccag tttttattgg tcgcttcggt
     5221 attacctcgg gttaatgaat attcgtaggc accggcgaaa atcctcgaat ccttaataaa
     5281 ttccccattt gattccccct caacgctgat aatctctatc ccatttaacg tgtaagctcc
     5341 gctgcctcct gcattggtaa ctttaactcg ggttgttcca gaggtatttc cattaataac
     5401 gactttatcc gtagctgaat tatcatcatc caattcagtt ctgagatgca atacgccatt
     5461 attaccaacg taattttcag ttattgttaa tcttgttggt tcgacatccc gtccatctgc
     5521 ccgggaaata tagaccgtac tattatcaac cgtgaggttg ctcaccgttg agtcagcttt
     5581 aatatcccag gtactattat ctgaaagcga cagataggta tgagtgttat catctgttga
     5641 aatattagct gaacccgtta tttttgaatt agttgcatcg acggtcaaat gacctttaat
     5701 attattgata aaaacatctg cagccgatga tacctcacta tccgtaatgg tcacgttact
     5761 cgtctcatct ttatggtaga ggatttcatt tgctgaccgt agtgagctac cagataaaat
     5821 atctaccgtt gcttcctgac catttatgga agactccagc acaattccag acccctgatg
     5881 cgcgactgca gatgtatttt tcagcgtcac cgtgccgcca ttataaattt tagcaatcgc
     5941 ggcgtcagga cccgttgtat aaatttctcc ctggttaaat gaaactgtcc cctggcgggc
     6001 agcgatggca tgagcattgt ccccatgcgt ggtgatatca acatcggtta atgtaacgct
     6061 ggcattggta taatcaagcg tgtcaccagc cttgacgaca gtgccattag cgtacgcggc
     6121 ataactttct gttccgtggg ttgtgatctc cccttttgcc atatccagtt ttgctatggc
     6181 attattttca ttacttcctg tatcggcagt actttcttca tcaagagaag atacacacgc
     6241 acacgcgctt ccgggagatg cacacgcaag aacgccgact gcgtgattac ccgcagtaac
     6301 aataaatgta tcatctccgt cagaaaaaga gggggtaacc gaaccgtcag agttgactct
     6361 tattatggtg tcagtattca ggtttccact cgtttgactt agcaaaccaa ttgagtaatc
     6421 ccccgttgtg acaatcgtgt ttttaccctg caaaataaca gcaccgccaa attcacttac
     6481 gattgcgcta gctgttaatg ctgggcttag aggactattg tcagaaggat ataaaccgcc
     6541 cgttgttaca atgacgccat tgttaatggt taacttacta ccctctcctg caacggatac
     6601 ccgtaacttc cattacccgt tgttataact agcgcattat caatatcttt tacttttcca
     6661 tcttttattg ccacagcatg tttatattca tcgtgctgaa taaactgttc agcatatgca
     6721 ttacccaccg ccagagcaat aagtcccaaa ctaaacccgg ggagtttttt cagtttcata
     6781 gattaactat ccattcattg gattaacaaa cattcctggt caaataacct atgagctcag
     6841 attaccaaaa cggagggtaa agacaaaaac aaaagcacaa aaaatttaaa tagataaaca
     6901 gcgacaatat ataaatgata aataagtcgg cattatcatt tttcacctta ttttcatgac
     6961 attgatcact ttggggtgac tattttgttt aacaaataac tcttcgacat caaaattatt
     7021 aatagttgcc atggtaatta tttcggtgat atataaaaac aactgtgata acaccaaggt
     7081 tgacaactat ttgcgcaata attcctttcc ttcaatgatc caggcgttca ctgccagcaa
     7141 cccattcagc cgcatactga cagtgagaaa ctgaaagatt attgctgatc cctgtggcta
     7201 acgagtcttt ctcttaaatc gattaaccag attgatcgcc agccaaacaa gggcattaag
     7261 caaatttatc gtcacaagca cttctcccag cccggtactg ggttttgcaa taaggatgac
     7321 caggagggtg atcagcataa atgcaatgga aaagatgata tagagagtga aataatccag
     7381 attattacta ttattcatgc tactaatggc aatcctgtca aaactttcaa acaattatag
     7441 caaacaaaat aatgaacaat agtaacaaga ggcaacgagc taattagctg tctataatta
     7501 caccgcacta tggccttact gatcaatttt cactcaacca caaccttcac cttcggttta
     7561 gaaaggataa ttttgaccag gtcgatgtat gtaacaggtt tatagaaata ataaccctgc
     7621 tgaaatgtga tattattttg gttcagatag ccaagttgtt ctttcgtttc gacaccttca
     7681 gcgacgatac tgatcgaaag tttacgcgcc agatccaata cacaatccag aattcgggtt
     7741 gattctgggt ctgcgtttac gcggccaacg aaactgtgat caattttgat ataatcaata
     7801 tgcaggtcat gaagataaga aagccctgag tagccagtac cgaaatcatc cagcgcgatg
     7861 acaaaaccat tttcatgcag aatgttcaac cgctgaacca gactttcatc aacattcaat
     7921 ggctcacgct cggtgacttc cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg
     7981 aaatttaaac actcgtcgac aaacgtcggc gaaataatat gcgaagcgct aaaattaatt
     8041 ccaatatgaa aaccttccgg cagtttactc gcgatagcgt tcatctgtct ggcaacctga
     8101 ttcatcaggc tttgtgtaag cggaacgatt aatccggatt tttcagcaag tggaataaat
     8161 gccgcgggtg aaatatatcc accgtgaggt tgtttccagc gggctaacac ctcaactccc
     8221 cgcaatgtcc cttcccgacc atttaccaca ggttggtaaa aagggacgat ctccccatta
     8281 tttattgccc gtcgcaggat ctcttcaggc gttgcgcttt tattgaaata tctatcaagc
     8341 aggaaggcag cggcgctggc gattaacaga ataaatatta aaatcccaaa gcctttatcg
     8401 ataagtctgt agaagctaaa gagtggcggc gggttaaaga taatagtgaa atgatatttt
     8461 gtggagtgaa ccttctcggt atagggataa tgtccattaa gcgtcatcac atcagccgtt
     8521 ggtccaataa tgtcatccgc cacgcgtaat acatacctta cccctttcaa gggtacatta
     8581 agcgccccgc gaatatgttg atcacttatc gtaaccaaaa tgcgcgtatc tgcaaattgg
     8641 ctctgataga gcaggatagg taatctatta acggtgtcga ttgccggagc caacagtaaa
     8701 ttactgtccg ggacaacagg aatacgagac agcaggaccc gattccca
//
LOCUS       DAJTYM010000136         8743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTYM010000136 DAJTYM010000000
VERSION     DAJTYM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(74..799)
                     /gene="fimC"
                     /locus_tag="OO575_003538"
     CDS             complement(74..799)
                     /gene="fimC"
                     /locus_tag="OO575_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6666364.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(836..1375)
                     /locus_tag="OO575_003539"
     CDS             complement(836..1375)
                     /locus_tag="OO575_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6666365.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(1440..1988)
                     /gene="fimA"
                     /locus_tag="OO575_003540"
     CDS             complement(1440..1988)
                     /gene="fimA"
                     /locus_tag="OO575_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6666366.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(2469..3065)
                     /gene="fimE"
                     /locus_tag="OO575_003541"
     CDS             complement(2469..3065)
                     /gene="fimE"
                     /locus_tag="OO575_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6666367.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(3543..4145)
                     /gene="fimB"
                     /locus_tag="OO575_003542"
                     /pseudo
     CDS             complement(3543..4145)
                     /gene="fimB"
                     /locus_tag="OO575_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            5599..6315
                     /gene="nanC"
                     /locus_tag="OO575_003543"
     CDS             5599..6315
                     /gene="nanC"
                     /locus_tag="OO575_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6666368.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6335..7441
                     /gene="nanM"
                     /locus_tag="OO575_003544"
     CDS             6335..7441
                     /gene="nanM"
                     /locus_tag="OO575_003544"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6666369.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            7506..8486
                     /locus_tag="OO575_003545"
     CDS             7506..8486
                     /locus_tag="OO575_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6666370.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2566 a         1888 c         1819 g         2470 t
ORIGIN      
        1 atgacattat tcctccggtt taaaatcacc cggcagtcaa ttctttttat tcaggcgaaa
       61 aattccccct gcgttattcc attacgcccg tcattttggg ggtaagtgcg ccataatcat
      121 ttattgttcg gtaagtaata ttgcttcctg catcagaagg caatttaacc gtgctttcgc
      181 ccattggagg caccaatgca ttttcaagaa cccgggttcc ggcattcaac tctgttaccg
      241 tcaggtaata gggtgtcggg ttaatcagcg tcagagaatt cgcgctacga cgaaatctta
      301 atttttctgc ggcctgatcg ggtggcaacg ctaatttagc cgggcgatag tacagtttaa
      361 tgcggctgat aattgcgagc tgtagcatat tctcagtcaa ttttgattta tccattgacg
      421 gaatcgcttt aacgttcatc cagaataaac tttcccggtc ctgtggcaat tggttatttg
      481 ttgcatcaag aatacgtaag gtattctctt tttttccctt catcgcaaac agaggaggcg
      541 tcacgataaa acgaccatcc tttacaccat cggcattttc cacccatgat tgaattaaat
      601 aggtactatt ttcatcatta tttgtcacgg caagttgcac ttgtttttgc cctgccggat
      661 aaattacgcg agtcgcacct aaggccactc ctgcttcagc gcgtccggca accatcattg
      721 ccatgaacat caggatacct gccagcaagc agaatgttat ttcctgcgat ttccttacat
      781 tgacgttttt attactcact gtcctgttcc tgttatcaca ttatctactg aacaattact
      841 gataggttaa agagaaccag gcctgggcat tggcgatgcc gccagtaacc cgacgcccgg
      901 tagcacgata tttggcgatg aaatgtagcg aagtagagcc tgagtaaagc cgtttccagt
      961 ttgctggagg acgattaatc ggtacgaggt ttccttcatc atcaaacaac gctacaccaa
     1021 tattggaggc tatccctggc ccctctccca cggagagcac atccggattt ttaccatccg
     1081 cgacaccgtg aaacgccaca cccacacgtt cactcaccac cgtgctacat tcccgtaaat
     1141 gaataacaaa aggcaccggt gcgctatctt cccctgccgc atgaaaccgg ttactgctga
     1201 tttgccccat attgaccgtc atttgtttat caccggcttc aatccggcaa gtttccgcaa
     1261 taatgacgcc ctgaaattgc atatttccgc cgggcagcgt ggtattccat ttatttccgg
     1321 ccagagcaaa cattggcagc aacgacgcta acagaaataa tctcttcctt ttcatcgtta
     1381 tttttatcgc acaagggtgg gcatccctgc ccgtaatgac gtccttgaac ctgggtaggt
     1441 tattgatact gaaccttgaa ggttgcatcc gcattagctg cacccggggt tgcctcgccg
     1501 attgcataat aacgcgcctg gaacggaatg gtgttggtac cgttattcag ggttgtttgt
     1561 gcactaaatg tcgcaccgtc cagcgtcaga gcattgcctg ttctgtctag gatctgcacg
     1621 ccaacgtttg ttgcactacc tgcagcagaa ctctgcagag ccagtacatc agtacgcgta
     1681 gcatcaattg ccgtacctaa gaaggcaaca gcggcttttg tggcaacagt ggtatcgcaa
     1741 tcattcagct gaatgttaaa accaacggca gagctggttg ctccagcctg cttcaggcta
     1801 gcggtacgaa cctgtcccaa ctgaacggtt tgatcaacag agcctgcatc aactgcgcaa
     1861 gcggcgttaa caacttcccc tttaaagtga atggtcccac catttaccgt cgtagtatcg
     1921 gccagagccg ctgcggaact gagggacaga gccgacagaa caacgattgc cagagtttta
     1981 attttcatgc tgctttcctt taaaaaaact atttctaaat cgacatgggc agtcgttctg
     2041 tacactttgt tttgtcaacg agtttgactg ccaacactgc acagttttcc cccaaaagat
     2101 gaaacatttg gggccatttt gactcataga ggaaagcatc gcgcacaaac tttttcagtt
     2161 tatttgttgg cttaatattc tattattatc tttatttata gatttttata ttgcatgagg
     2221 tggtttttgg agagaagaat gaggaagatg cgtcgagcca cagaaacgtt agctttacat
     2281 atagcggagg tgatgtgaaa ttaatttaca agagaaataa tttacatatc aaatagttag
     2341 atgctttttg tcgtttttta atatttttat gcttgagaaa aaatacgtaa cttatttatg
     2401 atatggacag tttggcccca attgtcttgt gtttatttgt tttttaactt tattatcaat
     2461 aagttaaatc aaacctcttc tctttttaat ttttcgttta tgagattatt tctttcccat
     2521 aatccggcaa aacgagcagc attactggcg gtataacgca cagtatggcg aatatttcga
     2581 tgcccgagat aatcctgaat taaacgggta tctgcaccac gctccgccag ttcataaccg
     2641 caagcatgcc ttaacatatg aggatgagtc tgcgttacgg ttccagcttc aataccggca
     2701 tcgcgaataa tgcgataggc ctgctggcga gaaagccgac tcccgcggcg agaaataaat
     2761 atagcgtcgg tccggtcagc gcctttccag ttagcacgtt cctgggtcca gcgttccacg
     2821 gcttcacgct catcaaaacg taacgggtga acggtagaaa atccgttctt cagtcggcga
     2881 atatttattc gaccttcatt aaggtcaagg tcctgataat gcagatcaag cagttcacta
     2941 atacgcatcc catgccgata tgccaacaga ataagacaat aatctctggc tcccgttgcc
     3001 ccgtaacaaa ccgcctgcat catggcctga acttctttac cggtaagata acgacgttta
     3061 ctcacaatag tagtactcct aactgagata tatttgaatg aatacctata ggaaacctca
     3121 atcggtcaaa attagcccaa tagataaaaa gataaccaca cacgaagtta tgtggctatc
     3181 agtcaattac attaaacatc atacaaaaat aaaatatcac tgatgtgaca acatctattt
     3241 tcattgattt gattcaatat aacaatagat tacagaggat taacttccag ttacttaaaa
     3301 aataaagaaa aaagtgtgga aacaggagaa tttaagtgat gtgtaaactt atgaatgatt
     3361 ttgtttattt attttgaaga acataaatca ttagattttg tatgggtatg gttaacttaa
     3421 tgtttattat gataattaag aatcacactt acaataacat caattaataa atggttaatc
     3481 atttttgata tatcgtaaga ataatgtagt ttttaacacc atccctggta tctcaactat
     3541 ctctataaaa cagcatgacg ctgtcgtcct ctggctctat cccagatgcc gtaaaaacgc
     3601 cctgcattgc tggcggtata ccagacagta tgacgaatat tgcgatgccc aagataatcc
     3661 tagataagtc gcgtatctat tcccatattc gccaaagcaa aaccacacga atagcgtaac
     3721 atgtgcggat gaatctccag tgacaacccg gcattaccac cagaagtcga gataatatgg
     3781 taaaactgtt gccgagaaag cggattcccc ttacgtgata aaaataccca ctcgctctca
     3841 gcatgcggat aagaagtacg gatactcaac cagtttttta aagcctgaac ttctttgttc
     3901 aatagcgggt gcgttgttga aaagcctttt tttaatcgat ggatatatat acactttgct
     3961 ttaagatcaa tatccgaaat cctcaatcga caaatttcac tcgcccggaa accatgaata
     4021 aaacaaagca aaatcagaca ataattacgt gttgcatgag gcccggtatt tgctgcttta
     4081 aggagtgact cgatttcact atgggtaagg aagttccttt ttttgttatc agccttattc
     4141 ctcatcgttt tcccttataa ttacagacgc gcactagctg tgctgggtta gcaataatcc
     4201 aacattttta tcctgattat gtttataaaa gcgaacgttt gcttaataac ttacgtaagt
     4261 ggaccagttc ttctgagcga acttaaatgg agtagcgata gttacttata ataactttgc
     4321 catacaaacg ccatgctctt gcatgctatg tacctttata tatttatcaa tctgatacga
     4381 atctaataat aaccctcatc attaatgcaa ctaatcttat ctatatatca tgtgatatgt
     4441 tctgtaacaa gtaaaatcaa cataaaaaat tcagcaaaat ttaatatttg ttaaactagc
     4501 cgtctcaaat atgacattat tagttaactg catttgccac aaaatggcta tttcatggaa
     4561 cgcagtaatt tcttagtggc tattatcatg ctaattctta gatccctagc gattattcct
     4621 gcctggttcg tcagcttagg ccatgagatt gttacattcc tttttatcct ggagatatgg
     4681 gggaacgcag gcgacgcttt ttcatctaca tccgttggta aatatagctt tggtcactac
     4741 gacttaagcc ttcctcaatc acagctattc gccgggcgat acggcaaaca aactaagtga
     4801 aacatcatcc tgaaaacacc aacatcaaca agcctctcca gatcgacatc aggagtgacc
     4861 agttacaagc cacaaaccaa gaaacatatt tcatgatgag aattatgctc agcctgatgg
     4921 cgagtaaacc cttattttca ccactctcac tgagttagct tgcaaagctt tctttgttta
     4981 gattctaaat aaattattta taaaacgtat agttaaaaga aaatctttag tttttgaaga
     5041 ccaccgcaag tgttcgtctg gcttcacatg gcatcttctt cttagaaaaa gatcgacata
     5101 ttttgtgacg cgaattgcaa atctggtttt gttgtatgga ttgcgtgatt tttgatctgg
     5161 tataacaggt ataaagatgc accacgatag tcaatgagac tgggcatctc gtaatctatg
     5221 gcaaacatca cttcagttct ttcacatcgg gtgatgaaaa tgcacttcag tctgaaagga
     5281 atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat gggttgagta
     5341 agtgatataa cttacgaata ttcaaatcaa ttaaaaatca gaagagattt tatactcagg
     5401 tatttaatct ggatctctgt ttatttaaat aatgttaaaa gagaattttc acaggagacc
     5461 ttatacaaaa aatataaaat acagttaccc gttgccaaag acactataag cctggcaaaa
     5521 aaatattaca caacataaat gctaattgtt tatgcgggct ttgtattgct ttctgtatcc
     5581 tacaaatgag tgaaatttat gaaaaaggct aaaatacttt ctggcgtatt attactgtgc
     5641 ttttcgtccc cattaatttc tcaggctgcg acactggacg tacgtggtgg atatcgtagt
     5701 ggaagccacg cctatgagac tcgactcaaa gtcagtgagg gatggcaaaa tggatggtgg
     5761 gcaagcatgg aaagtaatac ctggaatacc attcatgata ataaaaagga aaatgccgca
     5821 ctcaatgatg ttcaggttga agttaattac gcaattaaac ttgatgatca atggacggtg
     5881 cgcccgggaa tgttaacgca ttttagcagc aacggcacac gctacggacc ctacgtaaaa
     5941 ctgtcctggg acgcgacaaa agatcttaat tttggcattc gctatcgtta cgactggaaa
     6001 gcttaccgac aacaagactt atccggtgat atgtctcgtg ataacgttca tcgttgggat
     6061 ggatatgtca cttaccatat taatagtgat ttcaccttcg catggcaaac gacgctatac
     6121 agcaaacaga atgattatcg ctatgcaaac cacaagaaat gggcgacgga aaatgccttt
     6181 gttctacaat accatatgac gcccgatatt acgccataca tagaatatga ctaccttgac
     6241 cgccagggtg tttacaacgg cagagataat ttatcggaaa acagttatcg cattggtgtg
     6301 tcatttaaac tgtagtagac aggagacagt cacaatgaat aaaactataa tggcgcttgc
     6361 tatcatgatg gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc ctgtgccatt
     6421 taaaagtggt accggagcaa ttgataacga cactgtctac attggtttag gtagcgcagg
     6481 tacagcatgg tacaagctag atacacaggc caaagataaa aaatggactg cgttagctgc
     6541 attccctggt ggaccgagag agcaagcaac ctctgcattt attgatggca atctgtatgt
     6601 gtttggcggc attggcaaaa acagcgaggg attgactcag gtatttaatg acgtacacaa
     6661 atacaacccc aaaaccaata gctgggttaa attgatgtcg cacgcgccga tgggcatggc
     6721 gggtcatgtg acttttgtac acaacggcaa ggcttatgtt actggcggtg ttaaccagaa
     6781 tatcttcaat ggctattttg aagatctcaa cgaggctgga aaagattcaa ccgctataga
     6841 taaaatcaac gcccactatt ttgacaaaaa agcagaagat tatttcttca ataagtttct
     6901 gttgtctttt gatccctcaa cacagcaatg gagttacgct ggcgaatcgc cctggtacgg
     6961 aacggctggt gcggcggttg tgaataaagg tgataaaacc tggcttatta atggcgaagc
     7021 caaaccagga ttgcgaacgg atgccgtatt tgaacttgat ttcaccggta ataatttaaa
     7081 atggaataag cttgatcccg tctcatcacc agatggcgtc gctggcggtt ttgcggggat
     7141 aagcaatgat tctcttatat ttgctggagg ggccggattc aaaggttcac gagaaaatta
     7201 ccagaacggt aagaactatg cgcatgaagg cctgaaaaaa tcatatagca ctgatattca
     7261 tctttggcat aacgggaaat gggataaatc gggtgaatta tcgcaaggtc gggcctacgg
     7321 agtatcattg ccctggaata atagtttatt aattattggc ggtgaaactg caggcggcaa
     7381 agcggtgacg gattcagttt taatttctgt gaaggataat aaagtcacag tacaaaacta
     7441 acgcttcagg gccccggtaa aggggccctg ctatcaattt gtcatttgta ttacgcgaaa
     7501 attatatgaa cgcaataata tcacccgatt attactatgt tcttaccgtt gctggtcagt
     7561 ctaatgccat ggcgtatggc gaaggactgc cattaccgga cagggaagat gcgcctcatt
     7621 ccagaattaa acaattagcg agatttgcgc atacgcatcc cggaggcccg ccatgtcact
     7681 ttaacgacat tattccactg actcactgcc cacacgatgt tcaggatatg cagggttatc
     7741 accatcctct ggcaacgaat catcaaacac agtacggcac cgttggccag gcactgcata
     7801 ttgcacggaa attactgccc ttcattcctg ataatgcagg gattcttatc gttccgtgtt
     7861 gccgtggcgg atcggctttt accgcgggca gcgaagggac atattcagaa cggcacggag
     7921 ccagccatga tgcttgtcgt tggggaacgg atactccgct gtaccaggat ttagtcagca
     7981 gaacgcgagc cgcactggca aaaaatccgc agaacaaatt cctcggcgta tgctggatgc
     8041 aaggcgaatt tgacttaatg accagtgact acgcgtcaca ccctcaacac tttaatcata
     8101 tggttgaagc ctttcgtagg gatctaaaac aataccattc tcagcttaat aatattactg
     8161 acgcgccgtg gttttgcggc gataccacct ggtactggaa agaaaatttc ccgcattcgt
     8221 atgaagctat ttatggcaat tatcaaaata atgttttagc caatattatt ttcgtcgact
     8281 tccagcaaca aggtgaaaga ggactgacga acgcgcctga tgaagacccg gacgatttaa
     8341 gcacgggata ttacggttca gcgtaccggt caccggagaa ctggacgacg gcactgcgaa
     8401 gcagtcattt cagcacggca gcccgtcggg ggattatttc tgacagattt gtagaagcaa
     8461 ttttgcagtt ttggcgcgaa aggtgagcgg ggagtttatt catgggggat gtccttgttc
     8521 gttctcagag tagacaacaa aaaacaaatg agatcaaggt aatatctctt cacttgcggg
     8581 ctcgaacagc aggcaattgc caatataata atctggcaca agggtgaaaa acgcgcaccg
     8641 cataaaccgt tgttattgct tacagattag ccggatgcct taatagacat ccgcgccttt
     8701 tcgattatgg ctcggaaatc tacgaacatc tacacagttt act
//
LOCUS       DAJTYM010000137         8398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTYM010000137 DAJTYM010000000
VERSION     DAJTYM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1602)
                     /gene="torA"
                     /locus_tag="OO575_003546"
     CDS             complement(<1..1602)
                     /gene="torA"
                     /locus_tag="OO575_003546"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCR6666371.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDL"
     gene            complement(1602..2774)
                     /gene="torC"
                     /locus_tag="OO575_003547"
     CDS             complement(1602..2774)
                     /gene="torC"
                     /locus_tag="OO575_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6666372.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            2861..3553
                     /gene="torR"
                     /locus_tag="OO575_003548"
     CDS             2861..3553
                     /gene="torR"
                     /locus_tag="OO575_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6666373.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(3526..4554)
                     /gene="torT"
                     /locus_tag="OO575_003549"
     CDS             complement(3526..4554)
                     /gene="torT"
                     /locus_tag="OO575_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6666374.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            4637..7348
                     /gene="torS"
                     /locus_tag="OO575_003550"
                     /pseudo
     CDS             4637..7348
                     /gene="torS"
                     /locus_tag="OO575_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            7450..>8398
                     /locus_tag="OO575_003551"
     CDS             7450..>8398
                     /locus_tag="OO575_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6666375.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMT"
BASE COUNT         1976 a         2247 c         2181 g         1994 t
ORIGIN      
        1 gagatcgtta cgctcaaact gcgtggtcgc aggcagcacg atatcggcaa agcggcaggt
       61 tgaggtccac tggttatcta tggcgataac cgtttccagc ttgcgccagc cttcaataat
      121 gcggttgatc tgctgatggc gatggaatgg gttagttccg gcaaaaatac acattttcag
      181 cggcggcagt tttaccgatt taccgttcca gttgatcact ttccccggtt cgaggatcgc
      241 atcgataaaa cgggcaatcg gaatagtgct gctgtagcct ttgtagtcac tgttgtcgtg
      301 aacaggcgga atcgacgtag agccggagaa accactcaga ataacgcctt tacgccccgg
      361 cgtgcctgcg ccgttgtagt gccagccaaa accaccacct ggcaggccaa tttgccccag
      421 catcgccgcc agaaccacaa tcatccacgc ccactgttca ccgtgctgca tacgctgcac
      481 gcaccagcca gcaataattt gcgttctgtt cgccgccatc tgccgcgcca gcccacgaat
      541 ggtttcggca tcaatgccgg tcagtttttc agcccatgcg gcatctttcg gctgaccgtc
      601 tttctcaccc agcagatacg gcaggaactg ctcaaaaccc acacagtagt tagcgaggaa
      661 gtttttgtcg tacaggtttt cactgtacag cgtatgtgcc agcgccagtt gcagcggcac
      721 gtcagtttgc gggttaaccg caatgtgctt cacatgctcg cgccccagat actcatgggt
      781 ggatgtgaca accggatcga tgctgatgac ctcaacttca ccggcggcga ctttcgcttt
      841 tagctgctcg taatattcat aaacatcgtg atccgggcac caccagttcg cttgctggtt
      901 tttcagcaaa tcggagcccc acagcacaat ggttttgctg ttctgcaata ccagcggcca
      961 ggaggtttgc tgttcataca cttccattga accgactacg cgcggcagga tcacctgcgc
     1021 agcaccggta gagtaatctc cgcccgtacc aacgctatta ccatgcaagg caatagcttt
     1081 cgccagcatc cccgaagcgt tatggaacat ccccgtcgat tgccaaccac tggcggtcag
     1141 caaggcactc ggcccgtgag ttttctgtac gcgttccagt tcttcataga acatgtcgag
     1201 ggcttcatcc cagctcacgc gcacaaaacg gttatcaccg cgctgggagg tgtcgctcag
     1261 atggcgctta cgcagccagt ccacgcgtac catcggataa cgaatacgtg ccgcgttgtg
     1321 tacgtgatcc ggcaatccgg cgatcatttt cgacggatat ttatccagtt cgaacggttt
     1381 tgccgccaca aagcgaccat ccttcaccat cgcgcggata gccccccagt gcgacccggt
     1441 aagaatgccc tctttcgaga tgacagcctc agtcgccgct tgcgccgcag ttgcacggcg
     1501 cggcgttaac aatgacggcc ccagcatccc ggcgacggtt aagccgccga gttgtgccag
     1561 aaaacgccga cgtgatgcct gaaagagatc gttattgttc attatttttc ttccttctta
     1621 tcgccgtgag ccttacctgc ggtgtcagac gcattcattt gcagatattt caacaaagtg
     1681 cgttcttcac gtttatcgag actggtaaag ccaatcatgc cgttgagcgt gccgatccaa
     1741 ccgttagcgt caaagtgggc gatttccggt gcgccgtggc actcgttaca ggtgccgttg
     1801 tacaacgaat ccgcataggc ccagatcggt ttgatatcgt tcaccatgtc gcctttcttc
     1861 atccacgcgg tggcctgcaa cttgctccac tcggtattgg tgtcggcaac ggtggttttc
     1921 tccagcgttt tcacctgctg ctgcacatca ccacgaatcg aggcaacaaa gatgcgttta
     1981 cctgggaatt gggtgagtac acgctgacgt ccggcgcttt ccgtccagcc ggtaatttca
     2041 atttgcagcc agtcgccgtc acgtttaagg actttcactt ccgaagcagg cagcagagaa
     2101 ccagaggctt ctttatcgcc tttcgccgca taaattggct taatatcaat agagtacagc
     2161 gtgtcaccac tgtcattagc actggcgcgc agctcatcga actgcttacg gaagccgcta
     2221 ctcatatccg gtaactggtg ggcaatacct ttatgacagt cgatgcagga ttgattatct
     2281 ttcgctgcca ccttcatctg acgtgccgct tcaggatgct gcctggcatg atccatcgca
     2341 tcgtagttat ggcaggagcg acaggttgcc gagttgtttt ctttcattcg cgcccattca
     2401 cgctcggcaa gttccgcgcg tttggcttcg aatttttcag gtgtatcaat ggagtgggca
     2461 ataaaggtct ggtaaatgtc gttgctcgct tccagtttgc gcttcaccat gcctggaata
     2521 tccggcggga tatgacagtc atggcattca gctcgcacgc cggaggcgtt ctggaaatgc
     2581 accgactgtt tatattcttc atacaccggt tgcatactgt ggcaactgac acaaaattcg
     2641 gttgtgctgg tgactttgat cccaacgtgt ggcaatacaa tcagcgcaat gccaatcaca
     2701 atcccaattg cgaccagcgc cagtaccgac caacgagcac tgggtcggcg tagcgcgttc
     2761 cagagtttcc gcataataac ccctgtagaa ttatggttta gtgaagcgat cttaatgagc
     2821 aaatatgaac agatatgaac agaatgagta aaaccctctg atgccacatc acattgttat
     2881 tgttgaagat aagccggtta cccaggcgcg attacaatcc tacttcactc aggaggggta
     2941 taccgtttcc gttacggcga gcggtgccgg gctacgggaa attatgcaga atcagccggt
     3001 agatttaatt ctgctggata tcaacttacc cgatgaaaat ggcctgatgt taacccgcgc
     3061 cctgcgagaa cgctcaacgg tggggattat tctggttacc ggacgcagcg atcggattga
     3121 ccgtattgtt gggctggaaa tgggcgcaga cgattacgtc accaaaccgc tggaactgcg
     3181 cgaactggta gtacgggtga aaaatctgct ctggcgaatc gacctcgcgc gacaagctca
     3241 accgcacact caggactact gctatcgctt tgcgggttat tgcctgaatg tgtcgcgcca
     3301 tacgctggag cgggatggcg agccgattaa actgacccgc gcagagtatg aaatgttggt
     3361 ggcatttgtg actaatccgg gcgaaattct cagccgtgag cgtctgctgc gtatgctttc
     3421 tgcgcgtcgg gtggaaaacc ctgacctgcg caccgtcgat gtgttaattc gtcgtttacg
     3481 tcataaactc agcgcggatt tactggtgac gcaacatggt gaaggttatt tcttagccgc
     3541 tgatgtgtgc tgataaaaat agaccggacg aaatcccccc ggtgacagcg agcggcggat
     3601 atgttctcgg tcggcatttt tcggcgtcag aactaaaatc ggtgggctga cattatcaga
     3661 caccgattgc ccctgtaatt gcctgatggc ctgctcaact gccagttccc cctgccagac
     3721 catttgatcg ctggcagcca taatcactct tccccgcttc agcccgcgat acacctgatg
     3781 tgaaagataa aacgacacca cggtaagcgg cgttttcagg ttacgccctt cacccattgc
     3841 cgcctctgcc gcaatggccg ttccggcaac gacgtcgatt tctgggtggc gttccagcat
     3901 ctcctgcaac aggttgcgct ggatttcaat atcgttatca ccaagcgcaa tatcaacaat
     3961 acgcaccggg cttccggcaa tggctgcgcg aaaaccctcg accatctcct tactgccccc
     4021 ggcgttatcg ggtccgggca tcagcagcac gttcagtggt ttaccgtgcg cccattgcac
     4081 caaatatcgc ccaggttgat agcccatctg aaaccagggt acaccaacgc ggcttttcac
     4141 ctggggatca tcaagagcat ttaccagttc gatcaccggc agatttgcta cctgcttttg
     4201 caggtcggga aatgacgtcg tgctactacc gagcaaaatg gcctctgcgc cccactgttt
     4261 acactggtcg atttgtgctt gctgggtagc caactggctg tagccgcctg cctccagcac
     4321 ttttaaatcc acaccgtagc ggcgagcagc ctcctgcata ccatagttca acgataacca
     4381 gtatgaatct ttcaggctgg gataaagcgc gcacagtttc catacgcgtt tggctttaag
     4441 cggcatagag gcttgcaccg tgaaatgctg cgcatcatgc cagcgcaaca ggttatcagc
     4501 cgaaaatgcc gacaacatga aaagggaaag aagtaaaaat agcagtacgc gcatgatagc
     4561 ctcatcaata ataaggcttt atgctagatg cattccactt tgcgactcaa cctttttcac
     4621 cttaagtgca ccgaccgtga atttaaccct gacccgaaga ctctggatgg gctttgccct
     4681 gatggcgctg ttaaccctga ccagtaccct ggtgggatgg tacaacctgc gctttatcag
     4741 ccaggtggaa aaagacaata ctcaggcatt gattcctacc atgaatatgg cgcgccagtt
     4801 gagcgaagcc agcgcctggg aacttttcgc cgcgcagaac ctgaccagtg ccgataacga
     4861 aaagatgtgg caggcgcagg ggcgaatgct caccgcataa agcctgaaga ttaatgcgtt
     4921 gctgcaagcg ttacgggaac aaggttttga taccaccgct attgaacaac aggagcagga
     4981 gatctcccgt tcattacgtc agcaagggga actggtgggg cggcgtctgc aactacgcca
     5041 gcaacaacgg caactcagtc agcagatagc tgccgccgat gagatcgcac gcctggagca
     5101 aggtcaggcg aataatgcga caacttccgc tggagcgacc caggccggga tttacgattt
     5161 gatcgaacaa gatcagcgtc aggctgctga aagtgcactc gatcggctga ttgatatcga
     5221 tcttgagtat gttaaccaga tgaatgaact gcgccttagc gctctgcggg tgcagcaaat
     5281 ggtgatgaat ctggggctgg agcagatcca gaaaaatgca ccaacgctgg aaaagcagct
     5341 caataatgcg gtgaaaattc tgcaacgtcg gcaaatacgc attgaagatc cgggagttcg
     5401 tgcgcaggtc gcaacaacgt taactaccgt tagccaatat agcgatctgt tggcgctgtt
     5461 ccagcaggac agtgaaatca gcaatcacct gcaaactctc gcccaaaata atatcgccca
     5521 gttcgcgcag tttagtagcg aagtcagtca gctggtcgac accattgagc tgcacaatca
     5581 gcatggactg gcacatctgg aaaaagccag tgcacgcggg caatacagcc tgttattgct
     5641 ggggatggtt tcactttgcg cactgattat gatcctctgg cgcgtggttt atcgctcatt
     5701 cacgcgtcca cttgccgaac aaacgcaggc gctgcaacgg ctgctggacg gtgatattga
     5761 ctcccctttc ccggaaaccg ctggcgtacg ggagctggat accatcgggc ggctgatgga
     5821 tgcgtttcgc agcagtgttc atgcactgaa tcgccaccgc gaacagctgg cggcgcaggt
     5881 caaagcgcgt acagctgaat tgcaggaact ggtgatagaa caccgacagg cacgggcgga
     5941 agcagaaaaa gccagccagg caaaatcggc gtttctgacg gcgatgagcc atgagatccg
     6001 cacaccgctg tacggtattc tcggcactgc tcaactgctg gcagataacc ccgcacttaa
     6061 cgcccagcgt gatgatttgc aggcgattac cgactctggc gaatcgttgc tgaccatcct
     6121 caacgatatt ctcgattatt cggctatcga agcaggtggc aaaaatgttt cagtcagcga
     6181 tgagcccttt gaaccgcgcc cgctgctgga aagtaccctg caattaatga gcggacgggt
     6241 gaaaggtcgc ccgattcgcc tggcaacggc aattgccgat gatgtaccga gcgcgttaat
     6301 gggcgatcca cgacgtattc gtcaggtcat taccaaccta ttgagcaatg ccctgcgttt
     6361 tactgacgaa gggcacatta tcctgcgtag tcgcactgat ggcgagcaat ggctggtcga
     6421 agtggaagac agcggctgcg gtattgatcc cgcgaaactg gcagaaatct tccagccatt
     6481 tgtgcaggta agcggcaaac gcggcggcac cgggctgggg ctgactatca gtagccgtct
     6541 ggcccaggcg atgggcggcg aactgagcgc caccagcacg ccggaggttg gaagctgttt
     6601 ttgtttacgc ttgccgttac gtgttgccac tgcacccgtg ccaaaaacag ccaatcaggc
     6661 ggtgcgtctt gacggtttac gtttgctgtt aattgaagat aacccgctaa cccagcgaat
     6721 taccgttgag atgctgaaca ccagtggtgc gcaggttgtt gctattggca atgccgcgca
     6781 ggctttagag acactgcaaa atagcgaacc gtttgctgcc gcactggtgg attttgatct
     6841 gccggatatc gacggcatta cccttgcccg gcaactggca cagcaatatc cgtcgctggt
     6901 tttgattggc tttagcgccc atgtcattga cgaaacgctg cgccagcgta ccagttcact
     6961 atttcgcggg attatcccta aaccggtgcc gcgtgaagtg ctcggtcagt tactggcgca
     7021 ctatctccaa ctgcaagcca ataacgatct accactggat gtctcgcaac tcaatgaaga
     7081 cgctcagtta atggggacgg agaagatcca cgaatggctg gcattattta aacaacatgc
     7141 cctgccgctt ctcgatgaaa tcgacattgc ccgcgccagc caggacagcg agaaaataaa
     7201 gcgtgccgcg catcagctaa aaagcagttg ctcgagtctg ggaatgcgta gcgccagtca
     7261 gctatgcgca caactggagc agcagccatt atctgccccc cttccacacg aagaaattac
     7321 acgcagtgtt gccgctctgg aagcatgatt gaataagaaa gacctgaacg cgatttgaat
     7381 caatatcagc cagttaagaa taactagaat agtggcattt ggtatgcaac ttaacggact
     7441 ggcaaaatca tggcagagaa aaaaagaacc cgctggcagc ggcggccagg cacgacgggc
     7501 ggcaaattac cgtggaatga ctggcgcaat gccacgacct ggcgtaaagc gacgcaattc
     7561 ttactattgg caatgaatat ttatattgcc atcacgttct ggtattgggt gcgctattac
     7621 gaaacggcag gtagtacgac atttgtcgcc agaccgggag gtatcgaagg ctggctaccg
     7681 attgccggtc tgatgaatct gaaatatagc cttgcaacag gtcagttacc gtccgtccac
     7741 gccgccgcga tgctgttatt ggtcgctttt atcgtcatca gtctattact caaaaaggcc
     7801 ttttgctcat ggttatgccc ggttggtacg ctttctgaat taatcggcga tctcggtaac
     7861 aaactgtttg gtcggcaatg tgtccttccc cgctggctgg atattcctct gcgcggcgtg
     7921 aagtatttgc tgttgagttt atttctctat atcgcgttat tgatgcccgc tcaggcgatt
     7981 cactatttta tgttgtcgcc ctatagcgtg gtgatggacg ttaaaatgct cgatttcttt
     8041 cgtcatatgg ggaccgcgac attaatcagc gtgaccgttt tgctgattgc cagcctgttt
     8101 attcgccatg cctggtgtcg ttatctttgc ccatatggcg cgctgatggg cgtggtttcg
     8161 ctattgtcgc cgtttaagat tcgtcgcaat gccgaaagtt gtatcgattg tggcaaatgc
     8221 gcaaaaaagt gcccatcacg gatcccggta gataaattaa ttcaggtacg aacagtggaa
     8281 tgtaccggct gtatgacttg cgtagagtca tgtccggtag cctcaacatt gaccttttca
     8341 ctgcaaaaac ctgcagcaaa taaaaaagcc tttgcgttgt ctggctggtt aatgacgc
//
LOCUS       DAJTYM010000138         8367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTYM010000138 DAJTYM010000000
VERSION     DAJTYM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..184
                     /locus_tag="OO575_003552"
     CDS             <1..184
                     /locus_tag="OO575_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666376.1"
                     /translation="FLLSQQATNFYDFACYAKAKLNVEQLLYLGGTISHMYMKGGAIP
                     WQRYPFVTMISVERKG"
     gene            complement(331..2493)
                     /gene="uvrD"
                     /locus_tag="OO575_003553"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(331..2493)
                     /gene="uvrD"
                     /locus_tag="OO575_003553"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6666377.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2577..3293)
                     /gene="yigB"
                     /locus_tag="OO575_003554"
     CDS             complement(2577..3293)
                     /gene="yigB"
                     /locus_tag="OO575_003554"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6666378.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3293..4189)
                     /gene="xerC"
                     /locus_tag="OO575_003555"
     CDS             complement(3293..4189)
                     /gene="xerC"
                     /locus_tag="OO575_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6666379.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4186..4893)
                     /locus_tag="OO575_003556"
     CDS             complement(4186..4893)
                     /locus_tag="OO575_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6666380.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(4890..5714)
                     /gene="dapF"
                     /locus_tag="OO575_003557"
     CDS             complement(4890..5714)
                     /gene="dapF"
                     /locus_tag="OO575_003557"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6666381.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5751..5954)
                     /locus_tag="OO575_003558"
     CDS             complement(5751..5954)
                     /locus_tag="OO575_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6666382.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6143..7621
                     /locus_tag="OO575_003559"
                     /pseudo
     CDS             6143..7621
                     /locus_tag="OO575_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7618..>8367
                     /locus_tag="OO575_003560"
     CDS             7618..>8367
                     /locus_tag="OO575_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6666383.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPF"
BASE COUNT         1931 a         2308 c         2142 g         1986 t
ORIGIN      
        1 gtttttgttg agccagcagg cgacaaattt ttatgatttt gcctgttatg ccaaagcgaa
       61 actgaacgtt gagcagctgc tttatcttgg tggcacgatt tcacatatgt atatgaaagg
      121 cggggcaatc ccgtggcaac gctatccttt tgtcaccatg atttccgtcg agcggaaagg
      181 ttagaatgat agctttgtgt cagatactaa atatgctgct gatgccggat gcggcgtaaa
      241 cgccttatcc ggcctacatg acgttgcaat ttattgaatc tgaatgattt tttaggccaa
      301 ataaggtgcg cagcaccgca tccggcaacg ttacaccgac tccagccggg cgtaagctgc
      361 caccagccat ttgattccct ggccctggaa tgccacctgc aaacggctat gttcaccgct
      421 gccttccata ttgacgatgg tgccttcacc aaacttagcg tggcgtacac gctggccgag
      481 cttgtagccg ctgtcgttct cgaccatcgg cgtacccatc cgctgatggc tgaccgggcg
      541 gcttaccgtg gcgcgcaggc gcacctcttc cacacactct tccggcaact cgccaataaa
      601 gcgcgacggg cgatggtaaa cctctttgcc atacagacgg cgagtttccg cgtaggtcag
      661 cgtcagtttc tgcatcgcgc gggttacgcc aacgtaggcc agacggcgtt cttcttccag
      721 acgcccgcct tcatccagcg acatctggct tgggaacatg ccctcttcca taccgacgat
      781 aaacacctgc gggaactcca gacctttcgc cgagtgcatg gtcatcagtt gtaccgcatc
      841 ctgccaggta tccgcctgcc cttcgcctgc ttccagcgcc gcatgggata ggaacgcctg
      901 cagcggcatt aaatcttcgt cttcttcgtt gtagctgaac tggcgcgttg ccgtcaccag
      961 ttcctctaag ttttcgatac gcgtctgacc tttttcgccc ttctcctgct cgtacatggt
     1021 acgcaggccg gagtctttaa ttacccggtc agtctgtaca tgcagcggca tatcggcagt
     1081 ttcctgcgct aaggcgtcga tcagttccat aaaccgctgt aaggcgctgg cagcacgtcc
     1141 ggcgagggct ttttcctgca acagttcacg acatgcctgc cagagtgtta actggcgatc
     1201 gcgcgatgtc tgacgtacca cgtccagcgt ccggtcacca ataccccgcg ttggcgtatt
     1261 caccacacgc tcaaaggccg cgtcgtcgtt acggttggca atcaggcgca gatacgagag
     1321 cgcatctttg atttcctggc gttcgaagaa gcgcatcccg ccgtaaatac ggtacggcat
     1381 actcgcctgc aacagtgctt cttccagcac acgcgactgg gcgttactgc ggtagagaat
     1441 ggcgcactcg gcaagcgccc cgccgttgtc ctgccaggtt ttgatgcggt taaccacaaa
     1501 acgcgcttca tcgagttcgt taaaagcgca atagagggaa ataggctcac cgtccgcgcc
     1561 atcggtccac agttttttac ccagacgccc gttattgttt tcaatcaggg cgttagcggc
     1621 gctcagaata ttgctggtag agcggtagtt ttgctccaga cgaataattt cggcaccggg
     1681 gaaatcatta aggaaacgct gaatattctc cacctgcgcc ccgcgccagc cgtagattga
     1741 ctggtcgtca tcaccgacga tcatcacttt gccggtgtcg cccgccagca ggcggatcca
     1801 cgcgtactga atgttgttgg tatcctggaa ttcgtccacc aggatattgg taaaacgttc
     1861 gcggtagtgt tgtaggatat gcggcttgtt aagccacagt tcgtgagcgc gcagcagcag
     1921 ctcggcgaag tccaccaggc ccgcgcgatc gcacgcttcc tgatacgcct gatacacctt
     1981 ctgccaggtc tgctccaccg gattaccgta gctttgaata tgatgcggac gcaggccttc
     2041 atctttctgg ctgttgatgt accacattgc ctggcgcggc ggccactgct tctcgtcgag
     2101 gttcatggct ttgatcagac gcttaagcag gcgtagctgg tcttcgctat cgaggatctg
     2161 gaaatcctgc ggcagattgg cgtccatatg gtgcgcacgc agcaggcggt gcgccagccc
     2221 gtggaaggtg cctacccaca tgccgccctg gctggtgccc atcagttgtc cgatacgatg
     2281 acgcatctcc gccgccgctt tgttggtaaa cgtcacagcc ataatcgagt atggcgagca
     2341 gttttccacg ctcatcagcc aggcgatacg atgcaccagt acgcgcgtct taccactgcc
     2401 cgcgcccgcc agcaccagaa ggttgctgcg tggcgcggcc accgcttcgc gttgtttgtc
     2461 attaaggctg tcgagcaggt aagaaacgtc cattggcacc gccgcgtaaa aataggttgg
     2521 gcggagaagc gcaacgccct ccgccaaaaa gctgggtata tatacagatt tgctgattat
     2581 atcagcgagg tcagagatgc caaccgggaa atttccagat gtggcagtaa acggctgtcc
     2641 caggtttgca tcagatcgcc attttccggt ctgatccaac aagcctgcat tccgctgcga
     2701 attgccccac ccacgtcagt ggtgagatcg tccccaacat gtaagatctc gccgatcggc
     2761 acgttgagtt tttccgcagc caaaaagtac atatcgctga acggttttga gcgcccgtgc
     2821 gggccagcgc gcagcacaaa ctcaaaataa tcccccaaac caaacagttc cggctgggcg
     2881 ttaccgttgg tgatcgccac cagcggccat ttcttcgcca gctgttttaa ggtgtcgtga
     2941 gtttgctgcg ggacgtcgat tcggctgcgc catttggcaa agttaatcat tgctgcgtgt
     3001 gcgcctgcac tggcttcttc ggcactcagc ccggcgtcga gcatcgcttg ttcaatcgaa
     3061 cgaaaacgcc aacgtgtcac gtcgtgataa atctcgggtt ccgcttcccg taccgcctgg
     3121 cgcaggcgtt gcagatcttc attctggaag ctgcgcagcg ccggatgata attttgcaca
     3181 aaggtaagcg cctctcgctc ggtgcgcaaa atcaccggac ggttatcgta aagggtatca
     3241 tccaggtcaa aggtgagcgc cgagatgcgc cccaaaggcc ggtaaaaacg cattatttcc
     3301 cccgtttggc gcgtggatgc gccgcatcgt acaccgaggc aaggtgttga aaatcaagat
     3361 gagtatagat ttgcgtggtg gagaggttgg catgacccag tagctcctgc acaccacgaa
     3421 gatcgccgct cgactccagc atatgcgtag cgaacgagtg acgtaattta tgtgggtgaa
     3481 cgtgattatt cagcccttgt tttatgcccc attcggcaaa gcgtttctgc acattacgcg
     3541 cggagatacg cttgcccagt ttcgacagaa aaagcgcgtc gtcttcgcta ccaaacaggt
     3601 cgcgcaaatc aagccagtgc tcaatccacg ccacagcgtt gcgaccaatc ggcaggcggc
     3661 gctctttgct gcctttcccc atcacccaca cttcgccgga ctccaggtcg aggtgtttga
     3721 tatccagccc caccagctcg gaaagacgca gacccgcgcc gtacatcact tccagcattg
     3781 cacggtcgcg tacagcgagg ggatcgttga tatcaatatc cagcagccga ttcatatcgt
     3841 cgacgtcgat atttttcggc agatgacgcg gcgctttcgg tgccgaaact cctttcgccg
     3901 ggttagcttt gagttcgttc tggctgacca gccagtcaaa aaagctacgt agcgcagaaa
     3961 gccgtaacgc cagacttgct gctcccagcc ctttacggcg actgcgtaca gcaaaattgc
     4021 gtaccatcgc tgcatcgcat tgttgccagc tttgcaggcc gttttcgctg gcaaaattga
     4081 tgatcgcctc aagctgacgc tggtagttaa gtagggttat cgggctaagc tgacgctcca
     4141 cgctcagata acgtaggtag cgttctacat cggtgtgtaa atcggtcata cgcgttcaat
     4201 ccaacgctcc agaagctccg gcaacatcag cgcaatttca tgaagtaact gcgttccctg
     4261 cccttgttga tagtgactgg catcgcgact ggtaaacagc acaacaccca aatcagcatc
     4321 gcttcccagc atcgacatcg ccaccgatcc caccgctttc gcttccggta gcaccaccag
     4381 cagctccggt ccgttaagcg gcccaagata gtgctgttcc tgccccaaac gctgaatacg
     4441 cagcggttcg aaagactgac ggcttaatgc cagatgagtg tggttcgacg gcgcacctaa
     4501 gcgccagcga tctggaaaca ggcgcagact cgcaccagct aggccgagat cgcgcgccca
     4561 gcggtgaaag cgcatcagca tatcgtcgag actgctggcg gcggtgagac tgcgctgcag
     4621 gtagagtagg cgataaaaca gcccttcgtt ggcgatagcc tgctccatca acaacgccat
     4681 gttctcttcc agaacatgaa tgtgattacg tgcgcgggcc atgtgccact cgaccaacga
     4741 aacggtgccg cgtaccggat gcggcacacg tatcgcttct actgcgcgcg cattgcggat
     4801 aaaaaactca ggatttttaa tcagataatc gacaaccgcc cggtcatcaa gctccgtgag
     4861 tgtttcctgc agttcttccc ctggttgctt catagatgaa taaatccatc gtagacatgt
     4921 accgccgggc cagtcatata taacgggtga cccggacctt tccaggcgat atcaagacga
     4981 ccgccgggga gttccacgcg tacttcttcg tccagcaaac cttgctgaat ccctaccgcc
     5041 accgccgcac acgcgccgct gccgcaggcc tgggtttctc ctgccccacg ctcataaacg
     5101 cgtaaacgaa tatgctcgcg cttaaccact tgcataaaac cgatattggc gcgctccgga
     5161 aaacgctcgt gactttccag aacaggacca agcgtttcta ccgccgcggt atcgacatca
     5221 tcgacctgaa tcacgcaatg cggatttccc atcgacacca cgccgcataa gattgtctgc
     5281 tcggcggcgc gcataatata ggtcttttcc gctttgttag cgcgaaacgg cacggcggaa
     5341 ggttcgaagt tgggttcgcc catatttacg cggaccagat catcatcggt gacggtcaga
     5401 accatccgcc cgttggcggt gctgacgcgg atatcacgct tattggtcag tcctttcaga
     5461 cgcacaaaac gggcaaagca gcgcgcaccg ttaccgcact gcgccacttc actgccatca
     5521 gcattgaaaa tgcgatagtg aaaatccagt tcaggatcat acggcggctc aaccaccagc
     5581 agctggtcaa accctacccc caggtgccga tccgccaggc gacgaatcag ctccggtgaa
     5641 aaaaagacat tctgcgttac cgcgtcgacg accataaaat cgttgccaag gccatgcatt
     5701 ttcgagaact gcatcattta ctccaatcac gcgggtacag aaactgactt ttagtaattc
     5761 acctgggatg gaccatcgcc agtggcgcga tcgtttttat ccggcaccgt ggattgcgtt
     5821 tgcgtctcta ccggtttggt cggcggcggt gcgtttttat ctgcaggcgg gaaatagagc
     5881 ggacctttca gaccgcagcc cgtcaggctg aagagagtaa gtaatacagt gagtgcctta
     5941 aacacgtttt tcattatgga ttgcctgtaa agttcatcgc tttctgcttt ctatcatcgc
     6001 aggagaaggc gtaaaagcaa gcgtttagcg actctttatg aagtctgaaa agtgaggaaa
     6061 tgctgcgagt ataacgctta ctcgtgagga tttatgttct gctggcagtt tcgtgatcaa
     6121 cacctcgcgc caggatggct ttatgcacta tcgcaatgtg gtttgtttgt tgtcttgttc
     6181 attgttttta tcgtcggcat ggggatgtcg gctttccgca cctgaacata atatttacca
     6241 gaaacaggga aaaggcgtgg tgtacctgcg cccttataaa gaaacaaatc tttctctacc
     6301 tgacgtgaat tacaagagtt tgcgtcggtt acccaattta ttaatagacc cgacaacact
     6361 agacgaatgg gataaagaac cgcctctgac tgatttgaca accgactacc tctatgaggg
     6421 cgcacaggcc tggtatcctc attattcatg gcatagcgat ggtcgcaata tcctctacgc
     6481 gggtgaagtt gtgcaaaacc ctcccggcac acctcccgtc gatgtcgcct cgttcaaggc
     6541 atggggtgat tttgccgcag ataaacacag tctctatttt gaaggcaagc gcaccgatga
     6601 caatggtggt ggaaatagct tggatatcaa aacgctgcat caggtggaat tccggcctcc
     6661 ctgggatcct gacttactgg ggctgatatt acgtgacgct aatttcttgt atatcaatgg
     6721 gcatcgcctc gctgaccccg agagtttccg tgtgctggcg cagaagtcct gggatcagcg
     6781 aggaaagttt tccaccacgt ttaacccctg cattgccgta ccatttggcc cctgggatac
     6841 cctggctcgt acacggacca aaatcctgct caacggcgaa cagcttgatg ccgacccgga
     6901 caccttttcc gtcgtgcgct ggatgcccgg ctcactcttg acctggcgtg ataaaaacgg
     6961 gctgcagcgt aaagtcctcg acaaggaaaa tctggcgtgg gatgaagatt taacaaagca
     7021 ctgtctggat ttttctctgc tggaaaagaa agtgttctgg cgtaaaggac ctgcttgtaa
     7081 acaggaagaa ttacccggac tcgatccgga gcagtttcaa cccatcagtg atgctgtcgc
     7141 ccagcatcag gactcgcttt ataccatcat cgaaacagag tctggtaacc gtaagctgga
     7201 gatcgtgaaa cttgatgatc ccaatcttat tatcaacaaa cgtttcaacg ccgggaaacg
     7261 ccacggctat ttacttacgc gtgccgaagg gtagccatac cattccggtt tacacgtgtt
     7321 tgaatctgac ggaccgctga tcttactgga taaccgttct ccggatgaac gcgaagccca
     7381 tcttaatgac catccctttt tgcgcagatg gtatgcccgc gataaccgct acatttacag
     7441 ctttgatggc gcgcagctct ggcgataccg caccgctgat ccgaaacaag ttcgcttaat
     7501 ctggaaggaa caacattcgg gatatgtcta tggcgtaaat tacaaaacgg gatatctgga
     7561 cggaaaaatt actgatgacg gcgaatttat tcctgccccg cgcaatgagg cgacaaaatg
     7621 aagggtcaaa taatcacagt tgcactcatc ctgttgggac acacatttct gacgcctgct
     7681 gtacaggcca tagggttcga ctattacaat gatcacggtg ttatgtctta cggtaaaggt
     7741 tattgggggg ataagaaaat cattgcccag tttccggaga tgagcagggc cggtttgcgc
     7801 cttgtgaaaa atatctctgg gaaaacgggt tatatacctt ccgatgaaat aagcatagaa
     7861 aatgaggaat accactgggt gacagacggc cgcgttattc tctggcgtgg caaaatcgtt
     7921 agcaatccac cgggaacact cactgtcgat attgccagct ttcaggctat gggccgcttc
     7981 gcggtcgata aatatagcct ctattttgac ggacagcgca ccgaaagtaa tagcggcgcg
     8041 tcacgtgttg agctggcgac actaaaagct atcgaaggta actctaccac gctggtggat
     8101 agtaaaaatc tctacttgtc cggtcgacgt caggggagca gcagtgatgt tactgtatta
     8161 gaaaaaagat ggtggggtat taatccacgt cttatgagtg taaacagaaa ttcatattcc
     8221 aatgatctgc ttatccgcag tgggcagaat atttatttaa atggcgttca ccttacggcg
     8281 aatgcagact catttgagat aattcgctgg atacctcatt cactgctggt ttttcgcgac
     8341 aataagggtc tgcatcgtta tcccttt
//
LOCUS       DAJTYM010000139         8332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTYM010000139 DAJTYM010000000
VERSION     DAJTYM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..158
                     /locus_tag="OO575_003561"
     CDS             <1..158
                     /locus_tag="OO575_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666384.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO575_003562"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO575_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6666385.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO575_003563"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO575_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6666386.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1245
                     /locus_tag="OO575_003564"
     CDS             874..1245
                     /locus_tag="OO575_003564"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6666387.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1266..6127
                     /gene="traI"
                     /locus_tag="OO575_003565"
                     /pseudo
     CDS             1266..6127
                     /gene="traI"
                     /locus_tag="OO575_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6147..6893
                     /gene="traX"
                     /locus_tag="OO575_003566"
     CDS             6147..6893
                     /gene="traX"
                     /locus_tag="OO575_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6666388.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6948..7508
                     /locus_tag="OO575_003567"
     CDS             6948..7508
                     /locus_tag="OO575_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6666389.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7639..7851
                     /locus_tag="OO575_003568"
     CDS             7639..7851
                     /locus_tag="OO575_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6666390.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8095..>8332
                     /locus_tag="OO575_003569"
     CDS             8095..>8332
                     /locus_tag="OO575_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6666391.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1912 a         2086 c         2671 g         1663 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcttgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca ggtggaggcc agtgcctcca cctgacggac ggacagtcag
     1261 aaacggtgct gaccggtaat ctggtgatgg cactgtttaa ccacgacacc agtcgcgatc
     1321 aggaaccaca gttacacacg catgcggtgg tgactaatgt tacgcagtat aacggcgaat
     1381 ggaagacact gagcagtgac aaagtgggga aaacggggtt cattgagaat gtgtacgcta
     1441 atcagattgc ctttggcagg ctctaccggg aaaaactgaa agagcaggtt gaggcgctgg
     1501 gctatgaaac tgaagtggtc ggtaagcacg gtatgtggga aatgccgggt gtaccggtgg
     1561 aggccttttc cggacgctca cagactatcc gggaggccgt cggggaagac gcctcgctga
     1621 aatcccggga tgtggcggcg ctggatacgc gtaaatccaa acagcacgtc gatcctgaag
     1681 tcagaatggc cgagtggatg cagacgctga aggaaaccgg gttcgacatc cgggcatatc
     1741 gtgacgcagc ggaacaacgg gcatataccc gcacgcagac acccggaccc gcttcacagg
     1801 acgggccgga tgtgcagcag gcggtgacac aggccattgc cggattaagt gaacgcaaag
     1861 tgcagtttat gtatacggac ctgctggcca ggacggtcgg catactgccg ccggaaaatg
     1921 gtgtgattga acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc
     1981 ccctcgatcg tgagaagggg ctgttcacgt tcgggattca tatgctcgat gagctgtcag
     2041 tccgggcact cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa
     2101 gtgtcccccg gacggccggt tacagcgatg ccgtcagtgt tctggcacag gaccgtccgt
     2161 cgctggccat tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac
     2221 tggtcatgat ggcccgggag caggggcggg aggtgcagat tatcgctgct gaccgtcgct
     2281 cgcagatgaa cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc
     2341 agttgctgga aggaatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggcgaaa
     2401 aactctccct gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg
     2461 tcctgataac cgacagcggg cagcgaaccg gtacaggcag tgcactgatg gccatgaagg
     2521 atgccggggt gaacacatat cgctggcagg ggggagaaca gcgaccggcc accatcatca
     2581 gtgaaccgga ccgtaatgtc ggctggccgg agattttgcg gccagcgtga aagccggaga
     2641 agagagcgtg gcacaggtca gcggagtacg ggaacaggcc atactgacac aggccattcg
     2701 cagtgagctg aaaacacagg gcgtgctcgg acacccggag gtaaccatga ccgccctgtc
     2761 accggtctgg ctggacagcc ggagccgtta tctgcgggat atgtaccgcc cgggaatggt
     2821 gatggagcag tggaacccgg agacacgcag tcatgaccgc tatgtgacag aacgtgtgac
     2881 ggcgcagagt cacagcctga ccctgcggaa tgcgcagggc gaaacgcagg tggtgcgtat
     2941 ttcttccctg gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg
     3001 tgagcgtctg agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct
     3061 gcaggtggca tccgtcagtg aagatgcgat gacggttgtt gtgccgggac gggctgaacc
     3121 ggccaccctg cctgtgagcg attcaccgtt cactgcactg aagctggaga acggctgggt
     3181 ggaaacgccc gggcattccg tcagcgacag tgcgacggtt tttgcctccg tcacacagat
     3241 ggcaatggac aatgccaccc tgaatggtct ggcccgcagt ggtcgtgatg tccggctgta
     3301 ttcctcactg gatgaaaccc gtactgcgga aaaacttgcc cgccatccct cctttacggt
     3361 ggtttctgag cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct
     3421 gcagaaagcc gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct
     3481 ggaaagtaaa aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttgc
     3541 tgcagaagga accggttttg ctgacctggg aggggaaatc aatgcgcaga taaaacgggg
     3601 tgatttactg tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc
     3661 gtcgtatgag gcagaaaaga gcattcttcg ccatattctc gaaggtaagg aggcggtcac
     3721 gccgctgatg gagagagtac ctggcgaact catggagaaa ctgacatcag gacagcgcgc
     3781 cgccacccgt atgatactgg aaacgtctga ccgtttcacg gtggtgcagg gctatgccgg
     3841 tgtgggtaag accacacagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag
     3901 tgaacgtccc cgtgtcgtgg ggctggggcc cacacaccgt gcggtcgggg agatgcgcag
     3961 cgccggcgtg gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg
     4021 cagcggagaa actccggatt tcagcaacac gctgttcctt ctcgatgaga gctctatggt
     4081 gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgggctgt
     4141 ggccagcggt gacacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca
     4201 gcagacgcgc agtgctgccg atgtggtcat catgaaggag attgtgcgtc agacgccgga
     4261 actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcgc tgtccgggct
     4321 tgagcgtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc
     4381 cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat
     4441 gctgaaaggc gaggcttttc cggatgtccc catgacactg tatgaagcca ttgtccgcga
     4501 ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga
     4561 ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gagaactggg
     4621 gaaggtgcag gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg gggagctgcg
     4681 tcgtctctcc acctgggaga ataacccgga tgcccttgcc ctggtggata atgtgtatca
     4741 ccggattgcc ggtatcagta aggatgacgg gctgataacc ctgcaggatg cggaaggtaa
     4801 cacgcggctg atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt acaccccgga
     4861 caccatccgg gtggggaccg gtgacaggat acgcttcacg aagagtgacc gggagcgcgg
     4921 ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacactttc
     4981 tgacggtcag cagacccgtg tgattcgccc cggtcaggag cgggcagagc aacatattga
     5041 cctggcctat gccatcaccg cccacggtgc acagggggcg agtgaaacct ttgccatcgc
     5101 gcttgaaggc acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc
     5161 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     5221 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     5281 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     5341 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     5401 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     5461 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     5521 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     5581 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     5641 ccgtgataat cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc gtccgtggaa
     5701 tcccggtgcc atcaccggtg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     5761 gggagccgga aatggtgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     5821 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     5881 caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga
     5941 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tactgcgtga
     6001 accacaacgg gtgcgggaag cggtccggga agttgcccgg gaaaatttgc tgcaggagcg
     6061 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     6121 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     6181 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     6241 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     6301 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     6361 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     6421 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccttggt
     6481 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     6541 cgcgcagtgg gtggcgtaca gcagcggcca tactcctgat ggccctgtgg gggcctttgt
     6601 ccggcaccag ttacggtatt gccgggttgc tgatgctggc agtcagcaac cggctgtacc
     6661 gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg
     6721 cgcttaacct tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga
     6781 ccgttggtct ggtgttgtgt gccggaaaat cattaccccg tttctggccc ggagactttt
     6841 tcccgacgtt ctatgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt
     6901 ggtatctttg gccgcaagag gatgattcgt cagaggcaga acacagcatg acagagcaga
     6961 agcgaccggt actgacactg aagcggaaga cagagggaac agcgcctgtc cgcagccgga
     7021 aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg
     7081 agaaagcggc ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
     7141 ccatttatct gaacctgccc acgctggatg aggctgtgaa taccctgaag ccctggtggc
     7201 cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac
     7261 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga
     7321 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
     7381 acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcgggagcgc
     7441 gtctggcgaa aattcgtcgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
     7501 agaaataaaa aaagcctccc ccagagagag gaggcaggga aataaggctt aaaaggaaat
     7561 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     7621 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     7681 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     7741 gcggcagaag catggcgacg ggctgcctgt gttgcgccta ggacagactg gcagcagttt
     7801 gcccggaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag
     7861 cagccttcat tactctagga gtcagaaggc tgaaaggagt ttatgagatt tgcttcttaa
     7921 tatccggtcg ggcagtatga ccggacagtt ctgagtagca cagagtcttt tccgcattaa
     7981 gtcgattttt tatattttgt gattagtttc acaaaataag gtgttattca atgtgtgctg
     8041 cactattcag gggggctgaa atacaggtgg catttatctg atatggagaa taacatgagg
     8101 aaatatattc cactggcatt atttatcttt tcatggccgg tattgtctgc agatattcat
     8161 ggacgggttg ttcgtgtact ggacggtgat accatcgagg tcatggactc actgaaggcg
     8221 gtgcggatca ggctggtaaa tattgatgca ccggaaaaga aacaggatta tggccggtgg
     8281 tcaacagaca tgatgaaatc actggtcgca ggaaaaacgg ttaccgtcac at
//
LOCUS       DAJTYM010000140         8241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTYM010000140 DAJTYM010000000
VERSION     DAJTYM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1250
                     /locus_tag="OO575_003570"
     CDS             <1..1250
                     /locus_tag="OO575_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6666392.1"
                     /translation="PNTASPYASYLQYGHIAGIDEILEGKKPITDLGVKAIDDHTLEV
                     TLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYTLKDWVVNERIVL
                     ERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPD
                     EVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKAQGDMPAYGYTPP
                     YTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYNTSDLHKKLAIAA
                     SSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSNSSM
                     NTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPVYYYVNARLVKPW
                     VGGYTGKDPLDNTYTRNMYIVKH"
     gene            1336..2256
                     /gene="oppB"
                     /locus_tag="OO575_003571"
     CDS             1336..2256
                     /gene="oppB"
                     /locus_tag="OO575_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6666393.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2271..3179
                     /gene="oppC"
                     /locus_tag="OO575_003572"
     CDS             2271..3179
                     /gene="oppC"
                     /locus_tag="OO575_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6666394.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3191..4204
                     /gene="oppD"
                     /locus_tag="OO575_003573"
     CDS             3191..4204
                     /gene="oppD"
                     /locus_tag="OO575_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6666395.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4201..5205
                     /gene="oppF"
                     /locus_tag="OO575_003574"
     CDS             4201..5205
                     /gene="oppF"
                     /locus_tag="OO575_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6666396.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5258..5587)
                     /locus_tag="OO575_003575"
     CDS             complement(5258..5587)
                     /locus_tag="OO575_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6666397.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(5622..7082)
                     /gene="cls"
                     /locus_tag="OO575_003576"
     CDS             complement(5622..7082)
                     /gene="cls"
                     /locus_tag="OO575_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6666398.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7225..7398
                     /locus_tag="OO575_003577"
     CDS             7225..7398
                     /locus_tag="OO575_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6666399.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(7453..>8241)
                     /gene="kch"
                     /locus_tag="OO575_003578"
     CDS             complement(7453..>8241)
                     /gene="kch"
                     /locus_tag="OO575_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6666400.1"
                     /translation="YGALYLSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESAR
                     LFTISVIISGITVFATSMTSIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAIN
                     TILQLNQRGQNVTVISNLPEDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAI
                     LALSDNDADNAFVVLSAKDMSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEIL
                     ARVLNGEEINNDMLVSMLLNSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2064 a         1987 c         2073 g         2117 t
ORIGIN      
        1 atccgaacac tgcttctccg tatgccagtt atctgcaata tgggcatatc gccggtattg
       61 atgaaattct tgaagggaaa aaaccgatta ccgatctcgg cgtgaaagct attgatgatc
      121 acacattaga agtcacctta agtgaacccg ttccgtactt ctataaatta cttgttcacc
      181 catcaacttc accggtgcca aaagccgcta tcgagaaatt cggcgaaaaa tggacccagc
      241 ctggtaatat cgtcaccaac ggtgcctata ccttaaaaga ttgggtcgta aacgaacgaa
      301 tcgttcttga acgcagcccg acctactgga acaacgcgaa aaccgttatt aaccaggtaa
      361 cctatttgcc tattgcttct gaagttaccg atgtcaaccg ctaccgtagt ggtgaaatcg
      421 acatgacgta taacaacatg ccgatcgaat tgttccagaa gctgaaaaaa gagatcccgg
      481 acgaagttca cgttgatcca tacctgtgca cttactatta cgaaattaac aaccagaaac
      541 cgccattcaa cgatgtgcgt gtgcgtaccg cactgaaact aggtatggac cgcgatatca
      601 ttgttaataa agtgaaagcg cagggcgaca tgcccgccta tggttatact ccaccgtata
      661 ctgatggcgc aaaattgact cagccagaat ggtttggctg gagccaggaa aaacgtaacg
      721 aagaagcgaa aaaactgctg gctgaagcgg gttataccgc agacaaaccg ttgaccatca
      781 acctgcggta taacacctcc gatctgcata aaaagctggc gattgctgcc tcttcattgt
      841 ggaagaaaaa cattggtgta aacgtcaaac tggttaacca ggagtggaaa acgttcctcg
      901 acacccgtca ccagggcact tttgatgtgg cccgtgcagg ctggtgtgca gactacaacg
      961 aaccaacttc cttcctgaac accatgctgt cgaacagctc gatgaatacc gcgcattata
     1021 agagcccggc ctttgacagc attatggcgg aaacgctgaa agtgactgac gaggcgcagc
     1081 gcacagctct gtacactaaa gcagaacaac agctggataa ggattcggcc attgttcctg
     1141 tttattacta cgtgaatgcg cgtctggtga aaccgtgggt tggtggctat accggcaaag
     1201 atccgctgga taatacctat acccggaata tgtacattgt gaagcactaa tggcaatacg
     1261 tggggcagga gtgtcctgct ccacggtgtc tgatttttat cgcattacag aaggcacagg
     1321 ccagaaggta gggcaatgtt aaaatttatt ctacgtcgtt gtctggaagc gattccgacg
     1381 ctatttattc ttattactat ttcgttcttt atgatgcgcc tcgcaccggg aagccctttt
     1441 accggcgaac gtactttacc gccagaagtg atggccaata tcgaagcgaa atatcatctt
     1501 aatgatccga tcatgacaca gtatttcagc tacctgaaac aactggcgca tggcgatttc
     1561 ggtccatcgt ttaaatataa agattattcg gtcaatgacc tggtggcatc cagttttccc
     1621 gtttctgcca aactgggagc cgccgcattt ttccttgcgg taatactggg tgttagtgct
     1681 ggcgttattg ccgcattaaa acaaaacacc aaatgggact ataccgtgat ggggctggca
     1741 atgaccgggg ttgttatccc cagttttgtg gttgcgccat tattagtcat gatatttgcg
     1801 atcattttgc attggctgcc gggcggtggc tggaatggtg gggcgcttaa attcatgata
     1861 ttgccgatgg tggcgttgtc actcgcctat atcgccagta ttgcgcgtat tacccgtggc
     1921 tctatgattg aagtattaca ctccaacttt attcgtactg cccgggcgaa agggttacct
     1981 atgcggcgga tcattttacg ccatgcatta aaacctgctc tgttacccgt gctctcctat
     2041 atgggccccg catttgtcgg cattattacc ggttctatgg tcatcgaaac catttatggt
     2101 ttgccgggga ttgggcaact gttcgttaat ggcgcattga accgtgacta ttccttagtg
     2161 ttaagcctga ccatcctggt tggtgcgtta accattttgt ttaatgccat tgtcgatgtg
     2221 ctatatgcgg ttatcgaccc gaaaatccgt tactgatact ggagctcgcg atgatgttaa
     2281 gtaagaaaaa cagcgagacg ctggaaaatt tcagtgaaaa gctggaggtc gaagggcgca
     2341 gcttgtggca ggacgcacgt cgacgtttta tgcataaccg tgcggcggtt gccagtctga
     2401 tagtgctggt gctgatcgcg ttatttgtaa tcctggcacc gatgctttcc cagtttgcct
     2461 atgacgatac tgactgggcg atgatgtcca gcgccccgga tatggagtcc ggtcactact
     2521 ttggtactga ctcatccggt cgcgacctgc ttgtgcgcgt tgcgattggc gggcgtatct
     2581 cactcatggt cggtgttgct gcggcactgg tggcagtggt cgtggggaca ctttatggtt
     2641 cgctttccgg ttatctgggc ggtaaagtgg attcggtaat gatgcgtctg ctggaaatcc
     2701 tcaactcctt cccattcatg ttcttcgtca ttttgctggt gacctttttc ggccaaaaca
     2761 tcctgctgat tttcgtggcg attggcatgg tttcctggct ggatatggct cgtattgtgc
     2821 gtgggcaaac cctgagtctg aagcgcaaag agtttattga ggcggcacaa gttggcggtg
     2881 tatcgacgcc gggcattgtt attcgccaca ttgtgccgaa cgtactcggt gtggtggtgg
     2941 tctacgcatc gctactggtg cccagcatga tcctctttga atctttcctt agcttcctgg
     3001 ggttgggtac gcaagagccg ttaagcagct ggggggcatt gctgagtgat ggcgcgaact
     3061 cgatggaagt ctctccatgg ttactgttgt tcccagcggg attccttgtg gtgacactgt
     3121 tttgtttcaa ctttatcggc gatggcttgc gtgatgccct cgacccgaaa gatcgttaag
     3181 gagtgcagcc atgagcgtaa ttgaaactgc aactgtgccg ctcgcacaac aacaggctga
     3241 cgcactgctg aacgtgaaag atttgcgtgt cacctttagt accccggacg gcgacgtcac
     3301 ggcggtcaat gatttaaatt tttccctacg tgccggagaa acgctgggca ttgtaggtga
     3361 gtctggttcg ggtaaatcgc aaactgcatt tgcgttgatg ggcctgttgg ctgccaacgg
     3421 acgcattggc ggatcggcaa ccttcaatgg gcgtgaaatc ctcaatttgc cagagcgtga
     3481 actcaataaa ctgcgcgctg aacaaatctc aatgattttt caggacccaa tgacttcgtt
     3541 gaatccctat atgcgcgtcg gtgagcagtt gatggaagtg ctgatgctgc ataagaacat
     3601 gagcaaagct gaggcgtttg aagagtcggt gcggatgctc gatgcggtaa aaatgccgga
     3661 agcacgtaaa cgcatgaaaa tgtacccgca cgaattttct ggcggcatgc gtcagcgagt
     3721 catgattgcg atggcattgc tatgtcgacc taagttgctg attgcggatg aacccactac
     3781 agcgctggac gtcaccgtac aggcgcagat catgacgcta ttgaatgaac tgaagcggga
     3841 atttaatact gccatcatta tgatcaccca cgatcttggc gtggtggcgg ggatctgtga
     3901 caaagtgctg gtaatgtacg ccgggcgcac gatggaatat ggcaatgcgc gcgatgtctt
     3961 ttatcaaccc gttcatcctt attctatcgg tttgctcaac gcggtgccgc gtcttgatgc
     4021 ggaaggtgaa acaatgttga ccatcccggg taatccgcca aacttgctgc gattaccgaa
     4081 aggttgcccg ttccagccac gttgtccaca tgcgatggaa atttgtagta gcgcaccgcc
     4141 gctggaagag tttacgcctg gccgtctgcg tgcttgcttt aaaccggtgg aggaactgtt
     4201 atgaatgctg taactgaagg aagaaaagtc ctccttgaaa tcgccgatct taaagtgcac
     4261 tttgaaatca aagatggcaa acagtggttc tggcaaccgc cgaaaacgct caaagccgtc
     4321 gatggtgtca ctcttcgcct gtatgaaggg gaaacattag gtgtggtagg ggaatcggga
     4381 tgcggtaagt ccacctttgc tcgcgccatc atcggtttgg tcaaggcgac cgacggtcat
     4441 gttgcctggt taggtaaaga gttgctgggc atgaagcccg atgaatggcg tgccgttcgc
     4501 agtgatattc agatgatttt ccaggatccg ttggcatcgc taaacccgcg tatgaccatc
     4561 ggcgagatca tcgctgaacc actgcgtact tatcatccga aaatgtcacg ccaggaagtt
     4621 cgcgagcgcg tgaaggcgat gatgctgaaa gtcgggttat tgcctaacct gattaaccgc
     4681 tatccgcatg agttctccgg tgggcagtgc cagcgtatcg ggattgctcg tgctcttatt
     4741 cttgaaccga agctgattat ctgcgatgag ccggtttcgg cgctggacgt gtcaattcag
     4801 gcgcaggtgg ttaacctgct ccagcagctg caacgtgaga tgggattgtc attaattttt
     4861 attgctcatg acctggccgt ggtaaaacac atttccgatc gtgtgttggt gatgtatctc
     4921 ggccatgcag tagaactggg gacctatgat gaggtctacc acaatccact acatccttac
     4981 accagggcat tgatgtcggc agtccccata cctgatccgg atctggagaa gaacaaaacc
     5041 atccagttac tggaagggga attaccgtcg ccgatcaacc cgccttccgg ttgtgttttc
     5101 cgtacccgtt gcccgattgc cggtccggag tgcgccaaaa cacgtcctgt tctggagggg
     5161 agtttcagac acgccgtttc ttgcctgaaa gtcgatccgc tttaaaaaca ataagggctg
     5221 acagttgtca gccctttttc acgctaaaag cgattattta ttcccgccag atgatatggc
     5281 aaagtttgtg atctttttcg cggcataaca gaatgcgggc gaaaacatcg ctgatttcac
     5341 catcttcact gtccgccaga ccaatcacca cttcggcaaa aaagtccggg ttcaaatcga
     5401 aatcaacatg ttcctgccag tcttccgccg gatcgaataa ctccgcgccg ccgcgctctt
     5461 caaactgaag attgaacagc agaacatcag ctggatcgag attgtcggca gccagttcga
     5521 gaaaaatatc gtaggcctgc tcaagcgttt catcttcagt caggcgattg tttagatcca
     5581 tatccatagt cactacctgt ttaacctctg ttggcgacgt tttacagcaa cgggctgaag
     5641 aagtaaaaca gtcgctcggc gacacgttgc cataatggac gttttagcca taaacgggca
     5701 tcgagcagac gtgaacgcga aatataatcg tcctgaacgg cggcaaggtc tgcaccaaaa
     5761 cctttatcgt cgattgccaa ggtaatctcg aaattcagcc acagactacg catatcaagg
     5821 ttaacggtgc caaccagact tagttcgcca tcgaccagca cgctcttggt atgcagtaac
     5881 ccgccttcaa actgataaat tttaacccca gcagcaagca gttccgtaaa gaatgcgcga
     5941 ctggcccagc cgaccagcat cgagtcattt tttcgcggaa ggataatact gacatccacc
     6001 ccgcgctgcg ccgccgtgca aatcgcatga agtaaatcat cgcttggcac aaagtagggc
     6061 gtggtcatga tcaaatattc atgcgccgaa taagccgcag tcaataatgc ctggtgaatg
     6121 agatcttccg gaaagccggg gccagaagca attgtgtgaa tggtgtgacc gctggcctgt
     6181 tcaaacggca taatattgac atctggtggt ggcggcagaa tacgttttcc ggtttcaatc
     6241 tcccagtcgc aggaataaat aatccccatc gcggtggcga tggggccttc catacgcgcc
     6301 atcagatcaa tccattgccc tacgcccgca tcttgtttga agtagcgagg atcgaccata
     6361 ttcatgctgc cggtgtacgc gatgtaatta tcgatcatga tcatcttgcg atgttggcgc
     6421 aggtccatac ggcgtaaaaa cacacgcatc agattgacct ttaaggcttc gaccacttca
     6481 ataccggcat tacgcattag ctcgggccac gggctgcgga aaaaagccac actcccggcg
     6541 gagtcgagca tcaatcggca atgaatacca cgccgtgcag ccgccattaa tgattcagcc
     6601 acctggtccg ccatgccgcc gggctgccag atataaaaca ccatctcaat attatggcgc
     6661 gcgagctgga tgtcgcggat taacgcctgc atcacatcat ctgactcggt catcagctgt
     6721 agctgattcc ctttgacccc agcgatcccc tgacgacgct cgcaaagctt gaataatggc
     6781 gcagcgacac tgctattttc ttcggcgaag atatgcttac aggctttaag gtcgttaagc
     6841 cattttgcgg tggaaggcca catcgctctg gcgcgctcag cgcggcgttt gcctaaatgg
     6901 agctcgccaa cggcaagata ggcaataatt ccgactaacg gcagaatgta aataatcaac
     6961 agccaggcca tcgcggaggg aactgcgcgt cgtttcatta gaatgcgtaa agttacgcct
     7021 gcaatgagca accagtatcc cagaatggcc aaccaactca ccaacgtata aacggttgtc
     7081 atagattaga aatccttttg aaagcgcatt gttatgagtt tacgcatatg gattcatctg
     7141 gcaaataaaa acgcgggaaa agcactggtc agcgtcgggg gcgggggtat aatgaccatt
     7201 ctgttattgc atagagtagt taacatgaag cggagtagaa cggaagtggg gcgctggcgc
     7261 atgcagcgtc aggctagccg acgtaaatcg cgttggcttg aggggcaatc gcgccgaaat
     7321 atgcgtatcc acagcatcag gaagtgcatt ctaaacaaac agcgtaactc gttattgttt
     7381 gcgatctaca atatctaaat gtactgggca ccattgcggt gcccaataag tttactcttc
     7441 cgtctttaaa ctctattttt gcgccgattc tttactgtca gctttcgttt cttgttcatc
     7501 gttatcgctg aaaataccat gacctgagtt caacaacatt gaaacgagca tatcattatt
     7561 aatctcttca ccatttaaaa ctcgcgccag aatttcgctg ccaaacagtt gcggtgagag
     7621 aatgatatcc ggatgtacca tcttaatctt attcaggttt ttgctatcac tgacggcgag
     7681 aactgttttg acatcactgc tcatatcttt tgccgagagt acaacaaacg cgttatctgc
     7741 atcgttatca ctcagcgcca gaatggcccg gcaacgatcg attcccgctt tctttaatac
     7801 tgaactgtca ttactgtcac cggggataac atcagcgtta tcgcctaagc gttgctcaag
     7861 ttgcttgata tcatcttcag gcaagttgct gataaccgtt acgttttgtc cgcgttgatt
     7921 cagttgcagg atcgtattga tggcgagaat cgaatgtccg caaacaataa aatgatcttt
     7981 acgatgcatt gtatgattgt ttccttttaa aagtttgttg aatcccccgc ggataagcgg
     8041 accaaaaatt gaggtcatag atgtggcaaa aacggtaatg ccggaaataa tgaccgaaat
     8101 agtgaacaat cgtgctgatt cagaaacagg gacaatatct ccatagccga cggttgacat
     8161 ggtttctatc gaaaaataga acgcggtcat caaactttct attcgcggat taaaaccttc
     8221 gcttaaataa agcgcaccgt a
//
LOCUS       DAJTYM010000141         8093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTYM010000141 DAJTYM010000000
VERSION     DAJTYM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..958
                     /gene="espL1"
                     /locus_tag="OO575_003579"
                     /pseudo
     CDS             1..958
                     /gene="espL1"
                     /locus_tag="OO575_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1130..1237
                     /locus_tag="OO575_003580"
     CDS             1130..1237
                     /locus_tag="OO575_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666401.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1290..2048)
                     /locus_tag="OO575_003581"
     CDS             complement(1290..2048)
                     /locus_tag="OO575_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6666402.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2335..3264
                     /gene="pfkB"
                     /locus_tag="OO575_003582"
     CDS             2335..3264
                     /gene="pfkB"
                     /locus_tag="OO575_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6666403.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3365..3655
                     /gene="ghoS"
                     /locus_tag="OO575_003583"
     CDS             3365..3655
                     /gene="ghoS"
                     /locus_tag="OO575_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6666404.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDVFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3761..4621
                     /locus_tag="OO575_003584"
     CDS             3761..4621
                     /locus_tag="OO575_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6666405.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4662..5198)
                     /locus_tag="OO575_003585"
     CDS             complement(4662..5198)
                     /locus_tag="OO575_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6666406.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5345..6013
                     /gene="hxpB"
                     /locus_tag="OO575_003586"
     CDS             5345..6013
                     /gene="hxpB"
                     /locus_tag="OO575_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6666407.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6176..6766
                     /locus_tag="OO575_003587"
     CDS             6176..6766
                     /locus_tag="OO575_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6666408.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6899..8093
                     /gene="tcyP"
                     /locus_tag="OO575_003588"
                     /pseudo
     CDS             6899..8093
                     /gene="tcyP"
                     /locus_tag="OO575_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2112 a         1930 c         1931 g         2120 t
ORIGIN      
        1 gatgctcaga cctgggctga taggtatgtt cgcaactgtg caattgctat tctgccccta
       61 ttacctgtgg gagttctagg ggctattttc gcgggtattg catcacgaat gccatttgcc
      121 cctatacatc catcggcaat gttgttaata atggctacag gccagactca acagcttatt
      181 acattattca aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg
      241 cagaatagcg ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac
      301 gtgaaaatat ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat
      361 aatctggtta aattactgca aactaaaagt gctaacgaaa cacctggact ttatatctcc
      421 atgttgtatg gattcgatga aataatcgat atctttctga atacattagc cactccaata
      481 gcactaagag cttttaaaca aaaaactggt gatgagtatt ttagccatga aaatacatga
      541 tggtgagcca ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt
      601 cctctctaaa attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga
      661 tttattaaaa ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg
      721 taatgaagac gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc
      781 ttttagtcaa catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc
      841 tgttcatata gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat
      901 aaatgtcatc agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc
      961 agctatttac gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt
     1021 gaccatgtgc acaacgccac ccgacggctg taataacaaa aaatcccttg atgcctgtcc
     1081 cttttgttac actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa
     1141 acgctttgat aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat
     1201 gttgttgcta cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca
     1261 gaccgcataa cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc
     1321 cgacggtcgg tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa
     1381 gccaccttca gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc
     1441 gttgtgtctt ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac
     1501 tgccatgcat aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta
     1561 tatttgtcgt aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc
     1621 aaaaactgac gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg
     1681 tctgtcagcc agcttgcctg accaaataaa tagtcataat cagttaaatt aaagcggcta
     1741 cgcccgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
     1801 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
     1861 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
     1921 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
     1981 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
     2041 agcttcattt atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt
     2101 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
     2161 acttagcaat gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta
     2221 tttatatata tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt
     2281 cttcactttc cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta
     2341 cgtatctata cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt
     2401 tatcccgaag gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc
     2461 aacgtcgccc gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt
     2521 ggcgcgaccg gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact
     2581 gtagaagcca aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa
     2641 cagtatcgtt ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa
     2701 gagcaagttc tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca
     2761 ggtgtgaagc tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc
     2821 tgcatcatcg acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag
     2881 ttggttaagc ctaaccaaaa agaactcagt gcgctggtga atcgcgaact cacccagcca
     2941 gacgatgtcc gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc
     3001 gtttcccttg gtccacaagg agcgctgggt gtcgatagtg aaaactgcat tcaggtggtg
     3061 ccaccaccgg tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg
     3121 acactgaaac tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg
     3181 gggagtgcag ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa
     3241 atttacgctt acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc
     3301 aacctgtcgg caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg
     3361 aactatggcc agtggcgatc ttgtccatta tgtcataacc gtcatgttgc atgaggatac
     3421 attgactgaa attaacgagt tgaataatta cctgactcgc gacgtttttt tgctcaccat
     3481 gacggatgat gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac
     3541 ccaaagtgaa gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa
     3601 agatcctgaa atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt
     3661 ttttgggcaa taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc
     3721 gctaacctta tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg
     3781 tcttttgagc gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg
     3841 agaagtccat gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga
     3901 tgaaagggaa ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg
     3961 tagtaaaacc gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt
     4021 tctggtgatg gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg
     4081 tcagcaaatt gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga
     4141 taactcgctc tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt
     4201 tgctgaacaa cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg
     4261 caatatcgac gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc
     4321 ttctctgttg cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta
     4381 cattttcgac cctgcctgcg actggggtga ccgagagtgc gacctagcga tgttaccgct
     4441 gcatactgaa cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc
     4501 cgatttcctt gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt
     4561 atttggcggt cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg
     4621 atatgggttg aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga
     4681 gaaaagaaca cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac
     4741 tgcaggaaaa tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc
     4801 ccctcttccc ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag
     4861 cgcgcctggc gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg
     4921 atgaaaatca cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta
     4981 taccagaaca gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca
     5041 aagtcgagca tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc
     5101 agcaaagaaa tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt
     5161 ttcaaaacag caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc
     5221 gtcgtttcac ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg
     5281 ataggcgctt aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt
     5341 tgtaatgtca accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat
     5401 cgactcagaa cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga
     5461 tatctcccgt cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga
     5521 tctttggtac gcccggcaac cgtggaatgg gccaagccgt caggaagtag tagaacgggt
     5581 tattgcccgt gccatttcac tggttgaaga gacacgtcca ttattaccag gcgtgcgcga
     5641 agctgttgcg ttatgcaaag aacaaggttt attggtggga ctggcctccg cgtcaccact
     5701 acatatgctg gaaaaagtgt tgaccatgtt tgacttacgc gacagtttcg atgccctcgc
     5761 ctcggctgaa aaactgcctt acagcaagcc gcatccgcaa gtatatctcg actgcgcagc
     5821 aaaactgggc gttgacccac tgacctgcgt ggcgctggaa gactcggtaa atggcatgat
     5881 cgcttctaaa gcagcccgca tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga
     5941 tccacgtttt gtattagcaa acgtcaaact ctcatcgctg acagaactca ccgcaaaaga
     6001 ccttctcggt taatgaccag gggcagtgat cacgctgccc ctggttcttt atctgaattt
     6061 gcattcaact gacggattaa tcgtcaattt aagagaaaga gttacaccgt caccacttcc
     6121 gtgcactgta taaaaatcct atactgtacg tatcgacagt ttagtgagtt ttatcatgac
     6181 ggcggaaggt caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct
     6241 gacgcccgtg ctggcacagg gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg
     6301 tttgttaccg gacatcgatc acccaaagtc gtttcttggg cagcgattaa aatggatatc
     6361 aaaaccgatc gcccgcgctt ttgggcatcg tggttttacc cacagtctgc tggcggtatt
     6421 tgcgctgctg gcaacctttt accttaaggt tccggaaggc tggttcattc cggctgatgc
     6481 gctacaagga atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc
     6541 cggtgttccc ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca
     6601 aaagggcaac caactggaac gttttatctg catggcatta tttgtctggt cggtatggat
     6661 gccccattca ttacccgaga acagcgctgt tcgttggtca tcgcaaatga tcaatacctt
     6721 gcagatccag tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatca
     6781 cctttctgga ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct
     6841 tgcgccaaca ctatgactgc tacgcagtga tagaaataat aagatcagga gaacggggat
     6901 gaactttcca ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca
     6961 gacccgccat aaacagtgga gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt
     7021 ggtttttggc cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc
     7081 tgtacagtgg tttaacatcg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat
     7141 gccgttagtc ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt
     7201 aggcaaaatc agttttctga ccatcggtac gcttttgttt accacgctga ttgcggcgct
     7261 ggtcggtgtg ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg
     7321 tgcagaaact gcacgtctga acgccattga aagtaactat gttggtaaag tctctgatca
     7381 gagcgttccg cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg
     7441 agccaatccg acgtcaatta tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc
     7501 attgaaactg ctgaaggatg atgcgccgaa aggggaacgc gtcttaaccg ctatcgatac
     7561 cctacaaagc tgggtgatga aactggttcg ccttgtcatg cagttgaccc cttacggcgt
     7621 tctggctcta atgaccaaag tggttgcagg ttctaacttg caagacatca tcaaactggg
     7681 aagtttcgtt gtcgcgtcct acctcggtct gctgattatg tttgctgtgc atggcattct
     7741 gctgggcatt aatggcgtga gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac
     7801 gtttgccttt accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac
     7861 gcgtcgtctg ggcgttcctg aatccatcgc cagtttcgcc gcctctttcg gtgcaaccat
     7921 tggtcagaac ggctgcgccg gtttgtatcc ggcaatgctg cggtgatggt tgcgcctacg
     7981 gttggcatta acccgctgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt
     8041 agttccgcag gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gat
//
LOCUS       DAJTYM010000142         8074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTYM010000142 DAJTYM010000000
VERSION     DAJTYM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..248)
                     /locus_tag="OO575_003589"
     CDS             complement(<1..248)
                     /locus_tag="OO575_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6666409.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="OO575_003590"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="OO575_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="OO575_003591"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="OO575_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="OO575_003592"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="OO575_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="OO575_003593"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="OO575_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6666410.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="OO575_003594"
     CDS             complement(2964..3278)
                     /locus_tag="OO575_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666411.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3356..3466)
                     /locus_tag="OO575_003595"
                     /pseudo
     CDS             complement(3356..3466)
                     /locus_tag="OO575_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="OO575_003596"
     CDS             complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="OO575_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6666412.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4839..4976)
                     /locus_tag="OO575_003597"
                     /pseudo
     CDS             complement(4839..4976)
                     /locus_tag="OO575_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5058..5356)
                     /locus_tag="OO575_003598"
                     /pseudo
     CDS             complement(5058..5356)
                     /locus_tag="OO575_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5945..6104)
                     /locus_tag="OO575_003599"
                     /pseudo
     CDS             complement(5945..6104)
                     /locus_tag="OO575_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6108..7337)
                     /locus_tag="OO575_003600"
                     /pseudo
     CDS             complement(6108..7337)
                     /locus_tag="OO575_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7357..7704)
                     /gene="tnpB"
                     /locus_tag="OO575_003601"
     CDS             complement(7357..7704)
                     /gene="tnpB"
                     /locus_tag="OO575_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6666413.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7704..>8074)
                     /locus_tag="OO575_003602"
     CDS             complement(7704..>8074)
                     /locus_tag="OO575_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6666414.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
BASE COUNT         2068 a         1882 c         1773 g         2351 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag
     3361 tgaggttctg gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc
     3421 agcgattgta atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca
     3481 gtctgagagg gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct
     3541 ccacaccttc ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat
     3601 gtgttcatta catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat
     3661 gggaaactgg ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga
     3721 aattaagcag atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat
     3781 ttagcacact taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc
     3841 attattgtta agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt
     3901 tatatatgcc atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa
     3961 cccctcatta tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt
     4021 tttaataaaa tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa
     4081 caaagcagat tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg
     4141 tatttcggct gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca
     4201 aatcaatgaa aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat
     4261 gttctgtatt ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc
     4321 ttcattgatg gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc
     4381 tttattttca ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg
     4441 atgtaatcca tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat
     4501 aacctatttt tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca
     4561 cattgaatgg ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga
     4621 tatatcttat ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc
     4681 actcagaagt gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata
     4741 cgccagtcct taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct
     4801 ccggcttatc tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt
     4861 ttttctccgg gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc
     4921 catattgaac ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact
     4981 gacagcagtg acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg
     5041 acaaatttag taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta
     5101 cgatcacgca gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag
     5161 caaccattgc gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga
     5221 gtcatattag cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt
     5281 caggattttt gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca
     5341 actgtttttc attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag
     5401 agaattatac aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga
     5461 aaataagttt tcacgttgaa atcccacata gttagtatat gataaataat catggatgac
     5521 ctgattaacc tcttggggta gtgcttttat agaagaataa atattctact aatttaatac
     5581 atcacctaaa atagtattgt tttttatggt tggttgaagg tcgtggtgat gatgttagtc
     5641 cttttgagaa tattccagtg ccagatctta cttataataa aggggcttgg cagtttggat
     5701 aaactattgt tcttattcca ctaatacaaa ctttttgtaa agtatattaa atatacttca
     5761 tttcatactg aaatccccgg aagtattcaa tcaaacaatt acaccataaa aggaagaggg
     5821 ggccctaatt aagttgtcaa gcatgtttat gatacctgca gggtataaat tttctggctg
     5881 caagcagaca catataaaac gtctttattg atcctaccca cataatatgg acacagtcgt
     5941 aagtgaggct ctggttttca aattgttccg gactgagacc accacaccaa cggtgccgtc
     6001 gccagcgatt gtaatcgcac tcgatataac taaacactgt tgcccgcact atttcccgat
     6061 ggataatagt gttctccgtg gagacattcc attttcagcg aatgaatttt catctgttgc
     6121 tgtatccagt catacagtga ctgcatcagt ggcgcggctc tggcttttct tgccgccaga
     6181 cgctgttctg ctgaacatcc ccggacttct gcctcgatgg catacagttc accgatacgc
     6241 tgcagggctt ccgtggtgat gtcggttggc actcttgcat gcacatcgtg gatttttctc
     6301 cggacatgag ccatacatgc ggcttccgtt attctgccgg attcgtacaa cgcccggtaa
     6361 ccaccgtaag catcggcctg aagcactccg ctgtaaccgg ccagatgatt ctgtggatgg
     6421 atacctttcc ggtccggtga gtacgcgaac cagaccgccg gaggcatttc cgaaccggcg
     6481 ttacggtcat cacgaacgta gacccacagc cgggcagtcc ttgttttacc gctgcccggc
     6541 tcctggaccg gaacggggat atcatcggca tggactttac cgggcatcag cacatactgg
     6601 cgcaggatgt catacagcgg ttccagcagt tcagcaacag cacctgtcca gcgccccagt
     6661 gtggcacggc tcagctccac gccctgacga cggtatattt ctgactggcg gtataacggc
     6721 agatagtctg catattttcc agcaacaaca tgggccagaa gccccgctcc ggcataactg
     6781 cgtgcaatgg gttttgaagg caccggtgcc tgcacgatat ggtcacaccg gcaacaggcc
     6841 agtttcggac gttgtgtttc gataacctta aaggcgctgc tgataagctc cagctgctct
     6901 gacacatcac atcccagaga actgagttcg ccaccacagg ccgggcagca ttcctcttcc
     6961 ggacggatga cccgggtttc acggggaagt gaggctggta acggtttacg ggctgaagac
     7021 tggcgcaggg gagatggcag taccgggtca tattgctcac ccagcgtttc cgccatttct
     7081 tcctgaagtg cgctgattcg ctcctgtgct tccagtatct gccgttcggt ttttgcgcga
     7141 agtttttctg agcttttacc gaactgcatg cgctgcagtt tcgcaaccag cgccttcagc
     7201 cggttgattt cggaagcata agccgccacc cgctgtgaga gcaggcggtt gtatttcgcc
     7261 atctggcgga tggtgtcctg ttgcgtctgc aacagtgccc gcaggcgggc attctcatga
     7321 gcaagtgagg tgtccatatc ctcactttac aacgggttat atgcggatgc cagcgcgttc
     7381 cgttcgtttc gggtgtttcc agtcgatgcc ctcaagaagc atggataact gggccggagt
     7441 aaggtgcacc ttgccgtcac gggtgaccgg ccagacgaag cggccccgct ccaggcgtct
     7501 ggtaaagagg cacagtccgt cactgtcagc ccacaacact tttatctggt caccccggcg
     7561 tccgcggaag atgaacagat gcccggagaa cgggtcatcc ttcaggacgt tctgaacttt
     7621 tgagaccagg ccgttaaagc catttcgcat atcggtgata cctgcaacca gccagatacg
     7681 cgaacctgca gggaaagata tcatcagtgg ctgctccctt ttatttcgcg gataagtatc
     7741 tgtaataacg ccggcgtcag tttaccttta agcctgagag ttccggccgg cagaaccagc
     7801 tcacagcaca gactgtcgga cggtgtattt atctgctctg gttcctgtgc cggagccggg
     7861 attttattat ccggctccgg cgttaacgtc acgggaagca gtgcaggcat attttttccg
     7921 gaaggcagca ggccaccttt ccggtattga tggcgccagt tgaagagcag gttatcgttg
     7981 attccgtttt cacgggcgat ctgcgccaca caggctccgg gctgcagtga ctgctccact
     8041 aaagcgattt taaattcata aggaaaatta ggcc
//
LOCUS       DAJTYM010000143         7899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTYM010000143 DAJTYM010000000
VERSION     DAJTYM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(123..953)
                     /locus_tag="OO575_003603"
     CDS             complement(123..953)
                     /locus_tag="OO575_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6666415.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO575_003604"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO575_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6666416.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO575_003605"
     CDS             2379..2729
                     /locus_tag="OO575_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6666417.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO575_003606"
     CDS             2855..3415
                     /locus_tag="OO575_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6666418.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO575_003607"
     CDS             3418..6384
                     /locus_tag="OO575_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6666419.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO575_003608"
     CDS             6451..6828
                     /locus_tag="OO575_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6666420.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO575_003609"
     CDS             complement(7029..7688)
                     /locus_tag="OO575_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6666421.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1750 a         2239 c         2130 g         1780 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcg
//
LOCUS       DAJTYM010000144         7884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTYM010000144 DAJTYM010000000
VERSION     DAJTYM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..230
                     /locus_tag="OO575_003610"
     CDS             <1..230
                     /locus_tag="OO575_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6666422.1"
                     /translation="GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
                     EDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            239..958
                     /locus_tag="OO575_003611"
                     /pseudo
     CDS             239..958
                     /locus_tag="OO575_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1297..2067)
                     /locus_tag="OO575_003612"
     CDS             complement(1297..2067)
                     /locus_tag="OO575_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666423.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(2112..2546)
                     /locus_tag="OO575_003613"
                     /pseudo
     CDS             complement(2112..2546)
                     /locus_tag="OO575_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2558..2779)
                     /locus_tag="OO575_003614"
     CDS             complement(2558..2779)
                     /locus_tag="OO575_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666424.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2780..3463)
                     /locus_tag="OO575_003615"
     CDS             complement(2780..3463)
                     /locus_tag="OO575_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6666425.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3527..3838)
                     /locus_tag="OO575_003616"
     CDS             complement(3527..3838)
                     /locus_tag="OO575_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666426.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3835..4737)
                     /locus_tag="OO575_003617"
     CDS             complement(3835..4737)
                     /locus_tag="OO575_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6666427.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            5177..6136
                     /locus_tag="OO575_003618"
     CDS             5177..6136
                     /locus_tag="OO575_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6666428.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            6136..6531
                     /locus_tag="OO575_003619"
     CDS             6136..6531
                     /locus_tag="OO575_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6666429.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6714..7094
                     /locus_tag="OO575_003620"
     CDS             6714..7094
                     /locus_tag="OO575_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6666430.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            7140..7884
                     /locus_tag="OO575_003621"
                     /pseudo
     CDS             7140..7884
                     /locus_tag="OO575_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         2140 a         2021 c         1944 g         1779 t
ORIGIN      
        1 tcggtgaaaa cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct
       61 cagacaaggg aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga
      121 acgaagtgga gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt
      181 cgaaccaagt gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct
      241 acgtacttct cggggcgctt acgttgatgc agaagccata tgtgaagcgg cttcacgacc
      301 atcaatgcgt tttgtccagc caagaactga gacccagcaa gcaataaggg cattacaccg
      361 ggtcagagaa tcgctcatca gagataaggt gaaaacaacc aaccagatcc acggtttcct
      421 gctggaattt ggaatcagcc tgccgacagg ggatgccgta ataaaacggt tgtctctcgt
      481 attggctgaa cacgaaatcc ccgaatatct gagccgcctt ttggtgaggt tgcataccca
      541 ctacctttac cttgttgagc aaatagcaga gctggaatca gaattaagtc agtcaatcaa
      601 cgctgatgat accgctcagc gcattatgac aattcctggt gttgggccga ttactgccag
      661 cttactatcg tcgcaacttg gtgacgggaa acagttttca tgcagcagag attttgcggc
      721 ttccacaggg ctcgttcccc ggcagtatag cactggagga aaatcgactc tgttgggtat
      781 cagtaagcga ggtgataaaa atttacgccg gttactggtt cagtgtgccc gctcattcat
      841 gatgcagctt gaacgccagc atggaaaact tgctgagtgg gttcgggagc aactcaacaa
      901 gaagcattcg aatgtggttg cctgcgctct ggcaaataaa ctggcccgaa ttgcctaggc
      961 aatcactact cagcaaaaag aatatcaggc ctgatatata gatgctgaca aaactcaatt
     1021 agcttattgc aaacaataag atacaagtta ccgtctggtt ttgcgaagat tgattattga
     1081 tgacatgaac ggcaaatcga cctgattaaa aacctaacag gggaaaatgg ctcactgaag
     1141 ccgaggggat ttttaggatt atcaggtgcg gaactcatcg aggcgcgggc atcccttgcc
     1201 cacagagacg ccggatagat ttaagcaagc ccaccattaa tcaaaaatca gtgttgcaaa
     1261 aacgggggtg accatagatt ttccccgcca tcgtcatcat ttccggattt ttgccagggc
     1321 atccgccatg ctgcggattt cggcccgggc ttcccggaca acttcccgca gaacaccacg
     1381 gtcaaaccac cggcggggcg gttcatccgg tatacgcagc acatcgcagg ccagcccact
     1441 ggacaggata tgctcaatac aatcgctgac cagatacatt tccaccgccg aaaaacgtgc
     1501 gccccggttc aggatggcat cgagcaccac gggcaggttt tcatcgtctt catcgagcca
     1561 gcaggggtga tcgctttcct tcacgaccag gcgaaagcgg cgcgtctggc gggtcagcaa
     1621 cgtctgaccg cttgtggtca gcgccgcccg gatacgccgc gcatcttctg ccccccggca
     1681 actgacactg catactgcat cgcgcaccgc atcaagaact gccctgattt cgtcttcggt
     1741 cagccattca ccggcatacg ggaaagacac ctgcgttccg tccgccggaa gtaccggcag
     1801 cgactgccac tgactgccgg acagaacaag ttccgctccg gttccggtca gtttctgaat
     1861 ggcggaggcc aggtgcggcg gcagcacgcc atcgtggcgg ttaacgagta acgtcgccag
     1921 ccggggcgca tccagccgca cgtaaatgct gatccagccc ggatagttcg ggttacattg
     1981 gtcaacatcc gtctcttccg gccagccctg cgccagcagc aggttacggg cgtgacgaca
     2041 aatcagagca ttcagcgttt cattcataat tcatgaactc cctgtatcgc cgcccacagg
     2101 cggcgataac atcagtcgtt ttcgtgtgtg ttgtttttca gcaggctgcg ggcctgatca
     2161 accatcgcct gtcggcgggt aacgtattcc gggaggggga aaccgtgatc ccgggcagcc
     2221 cattcacgtt tccacagtgc ctgttcagcg atctgagtca gggtctccag cagttcagca
     2281 gggacggtca cctggcggtt ttcgcgcacg ttgctgataa tctccatcgc cgcggcggaa
     2341 gaaataccgg actcaatccg ctagtcttcc gggatgtcct ccaggtgcgc caggacggtc
     2401 ctgatttcat gatcggacag ggaaatatcc attccgtcag cgagggcctg aatctcttcc
     2461 ggggaccaga taaccagcat cagcggcgtg tctcccgaat actttgctgc cagcgcccgg
     2521 catagcgttt cgcatgttcc gtacatactc tctcccctca atctttgaag caggaatcca
     2581 gttccagaat ttcggtatat gcctccatca agaggcggtt tgcgcgaaac aacgtctgac
     2641 gcaccagacg ttccggcagg gtgtcagcat taaagctgta ccgccgctcc atctcattca
     2701 tggttgtacg caggtcgcac atggcattgg ccacctccgc cacttcggcg cgaagttttt
     2761 cgtgtccggc atagttcatt tacgcagcct ccggcataaa ccagtcatca ttcgcggccc
     2821 cctgctgcat ggcccgctgt acggcagcca gtcgttgctg tccggcacgg tgatactgct
     2881 caagcagctc gataccgata taccggcgtc cggactggag ggcggcgacg caggttgaac
     2941 cgctgcctgc aaacgggtcc agcactatcg cattcgggtg tgtgaagctc tcaatcagcg
     3001 gttgcaggct ggtaacgggc ttttccgtcg ggtgatggcg gttgcccgaa tatttccagc
     3061 ccaacacgtc cggcagcggg ttttgtggca gacgtggacg gccttttgcc aggatgtagg
     3121 cgcattcgtg gcgatagccc acatatgcgg ccttcgatgt gtagtttttg gtgaagacca
     3181 ggtgaccaac aacgctgaat cccgcatttt tccaggcaga cataaagcga tcgacgcggt
     3241 tccagccata gaagctcacc atcagcgcgt cttttttgag tacgcggtac atttcattac
     3301 aggccggttg cagccattca tcagttttat caccagcaat ggtacgcccc tgacggtcac
     3361 gaaaaccgac gagatatggc ggatcggtga ggatgaaatc aatggcgtta tcaggaatac
     3421 gggccataac atcgatgcag ttaccgagga caaaacggga cataacagat ccttctctta
     3481 cagagggcac ccctgtggtg cccgttcccc gctgcctgtg ggctgttcat cgcttccggt
     3541 aacgggtcag gccgaccgtg gaataccgca gccgtcgtct ttttgacagc aaggttatgc
     3601 cgcgaaagcg gcttgaaggg cgttcatcag aaaagggcga cgggctggcg tacactgtca
     3661 ccgacaccag ccgggagcgg taaaagcgcc cgtcccgtgc ccgccggacg cgggacactg
     3721 accacaggca gcgtttatgc cgcgccgcgt cagccggcgc agtaaaaaga ggagcacggc
     3781 gacatccttt gtcggaaaag aaacgggcgg ttgcccgccc gtctccgtca tacttcatcc
     3841 gccataatgc gccacattgt cgactatcca cgcaggtccg gtgacttccg gctgctcatc
     3901 gtcatctgtc ggggaagacc attcaagttc cccccacagc acattgacga gctctccacg
     3961 ctcatacaac tgccagccac agaaatcaca gccctgttcg gcataccagt gctccagcgt
     4021 gcagctgaaa cggcggctta attctgcaat aacgtcgctt tccggctgac accacggagt
     4081 gtcgaaatcc acctgaatga agttttctcc ctgactgctg atattcaggt catatccaca
     4141 aggccacttc acgccatacc gttccgtata ccagtgatat gcagaaggaa caccgttcag
     4201 aacaccacca ttaaagccgt tcacttcaac atccaggcgg gtcggcagaa ccgtcagcag
     4261 gtcaaacggc atggtttttt ccggcactac gttgtcacac agacggttcc accacaccga
     4321 tacatcctcg ttaccccata tgtcacacca gtcacctctt tttgcggtga aatgcacagt
     4381 gatggtttcc ctggcatcat ccggtaatcc ttcccaacga cgctggccag taccgctctg
     4441 taaccagagt tcatgcagca tcaggcagtt ttgttcatcc agcaacacac cgttctgcag
     4501 gtgtgtcagc cagcgggtga acgcgatatt ttccgtcgat acagcaccac gtccggcagc
     4561 cgtcactccg gggcactgtt cagagcggat attttcagtg atctgcagaa gtccggcact
     4621 tccggccaga aacagctgaa taccttcatt tgtggcgcgg cgataaagcg gtgtgactgc
     4681 accgctggcc agtcgtttaa tctcagcgat ctgtgccggc tcaccaggaa aatacatacg
     4741 attcgagcac cagtttggca tgggaattct cctctcacat gcggcctcca tcaggccgtg
     4801 ccgcacctgt aaccgggcga ccgcatacga atgcaggtgc ggccccgaag ccgcagcgcg
     4861 tggaggagga atgacgaaac gtgaccgggt gaggaggccg gcgacagcga cacacttgca
     4921 tcggatgcag cccggttaac gtgccggcac ggcctggtta aatagtatcc gtctcagttc
     4981 gtggcgcaaa atgagaaacc aataagaact caaaatgaac cacaacaaga acataaagag
     5041 actctaaaag agcaacaccg tttgacatag actcaaaatg agtcacaata agaaacaata
     5101 aaaaactcaa aaagatgcac cgacataaag gatgctcaat ttgagataaa cacaaactca
     5161 aaacgaggca aaaagaatgc taaaagtatc atgcgatgat ggttcaacta acgtaaaact
     5221 ggcctggctt gaagatggcg aggtaagaac cagtctgtca ggaaacagct ttaaagaagg
     5281 ctggaacccg ggactgttta atgccggcaa agtttacaat tacgtcgtgg atgaaaaaaa
     5341 atacacctat gatttgggaa gcactgctgt cataggtaca acacatgtca gttatcaata
     5401 cagcaccacc aatctgcttg ccatacatca tgcactgctg acatccggcc ttcagccaca
     5461 ggatgtagaa ctgacagtaa cattacctgt gaccgaattc tttgataatg acaatcaacc
     5521 aaatgaagaa agaatagaaa gaaaaaaggc aaatgttctc cgtgagatat ctctgaataa
     5581 aggggaaaca tttaaaatta aaaaagttaa tgtaatgcct gaatctttgc cagctgcatt
     5641 tgaatcacta aaaaaagata aagtaaacaa acttgaacgc agcctgatta tcgatcttgg
     5701 aggaaccact ctggattgtg gtttaattct cggtgcattt gaaggcattt ctgaaatccg
     5761 tggctattca gaaatcggga catcccgcat cactcatacc gtcatgaatg cgctgacgaa
     5821 agcctccaca ccatgtaatt attttattgc tgatgagtta ataaaaaaca gacacgataa
     5881 tgaatatctc cagacattaa taaatgatgt agctgaaata aaaaacattt ctcatgtaat
     5941 agacagagaa gtgaaatctc tggcagaaag catcaggcag gaaatttcaa cattctcagg
     6001 aatgaaccga atctatctca ctggcggagg agccgagctt atttaccctc acataaagca
     6061 atatttcccg aatctgaaag tgaacaaggt ggatgaacct cagtttgcat tggtgaaagc
     6121 aatggtgcat gcgtaatgga aagcagtgat ccaaaaaaaa gaaagaaagt tgtggcatac
     6181 ctgcacccgg cactttatcc gcaggacaac ctgacacaac aaacaataga ctctctccca
     6241 gtacaaatgc ggggagattt ctacagacaa tcccttattt gcggagcagc tctttattct
     6301 gttgcccccc gactgttaac gctaatcagc gtttttttca gtgagaaaat cactgcagaa
     6361 aatctggtga aacttataga acaaacaaca ggatacacat ccacatcaat tgatataagt
     6421 gtgttaaaaa acatcataga ggcatcttca gaaaacaaat cagagagcat cacttcaaaa
     6481 gatgatttcg aagaacaaac cagacgcaat ctttctatgt taaaaaaata ataaaagaag
     6541 agcaggttca gtcaattacc tgctcttctt ttatacatac taaaaaatca gatttttacg
     6601 aatattcaga ttatcataaa aaatgcaaca gacaggaatg caataatttt gcattatctc
     6661 gttgcttgta atgcaaatat gcattattgt cagtgtgtga caataacaga gagatgacga
     6721 tggcgaccat taacgcacgt attgatgatg atataaagaa ccaagcagat gaagtgctga
     6781 agctgatgaa tatcagtcaa actcaggcta ttgcagcttt ttatcagtat attactgaac
     6841 aaaagaaact gccatttgtg atcacctcaa ttgtaaaaac gccgcatgat ttattacgtg
     6901 aaagtacaga catgttagct gaagctcttg cagtgatttc caatctacag gtgtggactg
     6961 aacagcagga tgggattgga aaagcaaagc taatggagta ttaccgccgc ctcgacgcac
     7021 tgtactgttg tgcaaaagag aaaataggtc ttctttcaga taaccgggat gctgaactag
     7081 gctgtgtccc ttaatcatct gagttatagt aactgtctgt ttacacaaca tattgaacta
     7141 tggctcgcta cgaccttccc gatgaggcat ggaccatcat caagcccctg ttacctcctg
     7201 aacccgccac accacgggcc ggacgcccat gggctgagca ccgtaaaatc atcaatggca
     7261 tgttctgggt gttatgttcc agtgcaccat ggcgcgattt acccgaacga tatggggcat
     7321 ggaaaactgt ttataaccgc tttaaccgat ggtcaaagtc aggcgtgatt aatattattt
     7381 tcaacaggtt gctttcgcta cttgatgcaa acggctttat tgactggtct gccaccgcgc
     7441 tggatggcag taatatccgg gcgctgaaat gtgccgccgg tgctcaaaaa aacatcccga
     7501 tatcgacgga gataatgggc cgggtcgctc tcgcggcggt tttggcacca aaatccatct
     7561 ggcaacagac ggaagtggcc tcccgttaaa tatcgtgcta agccccggac aagctcatga
     7621 aagccagttc gcgcaacgac ttctggacgg gattggcgtt cagcgtcaga acggcagcat
     7681 gaaacgccgt ggacatgcgg tactggctga taaagcgtat tcagggcgcg cattgcgcaa
     7741 tgagctgaaa aataacggta taaaggcagt aatcccccga aagtcaaatg agaaaatggc
     7801 atcggatgga cgtgcacagc ttgatcgtga tgcgtactgc aatcgtaatg tcgttgaacg
     7861 gtgctttggg cggctgaaag aata
//
LOCUS       DAJTYM010000145         7878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTYM010000145 DAJTYM010000000
VERSION     DAJTYM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(4..697)
                     /gene="ospZ"
                     /locus_tag="OO575_003622"
                     /pseudo
     CDS             complement(4..697)
                     /gene="ospZ"
                     /locus_tag="OO575_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            753..905
                     /locus_tag="OO575_003623"
                     /pseudo
     CDS             753..905
                     /locus_tag="OO575_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2286..3233
                     /gene="icsP"
                     /locus_tag="OO575_003624"
     CDS             2286..3233
                     /gene="icsP"
                     /locus_tag="OO575_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6666431.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3795..4167)
                     /locus_tag="OO575_003625"
                     /pseudo
     CDS             complement(3795..4167)
                     /locus_tag="OO575_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4411..4530
                     /locus_tag="OO575_003626"
                     /pseudo
     CDS             4411..4530
                     /locus_tag="OO575_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4780..5646
                     /gene="ospB"
                     /locus_tag="OO575_003627"
     CDS             4780..5646
                     /gene="ospB"
                     /locus_tag="OO575_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6666432.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5975..6715
                     /gene="phoN2"
                     /locus_tag="OO575_003628"
     CDS             5975..6715
                     /gene="phoN2"
                     /locus_tag="OO575_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6666433.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7075..>7878)
                     /gene="ospC4"
                     /locus_tag="OO575_003629"
     CDS             complement(7075..>7878)
                     /gene="ospC4"
                     /locus_tag="OO575_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6666434.1"
                     /translation="YIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDE
                     VSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDR
                     IINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALA
                     YSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEY
                     FINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2471 a         1479 c         1475 g         2453 t
ORIGIN      
        1 acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca tttgttttat
       61 gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac gagcaataat
      121 aatctcagat ttaatagact ttaatctctg gcgactcatc tcttaacact tgcataatct
      181 ctctttttat atcatatagt tctataaacc gatttgcccg tagttctcct tgggaagaat
      241 catttctgaa tacaacaatt atatacaaaa aatcactatt ttctttagag aagatcttac
      301 aactaatctc tgggagctca cataatgcat gtaagtcagg atggtacaaa ctatccacag
      361 aaaattgccc cataggacgt tttccagata taactgtcaa ccagcttcct tccggtgtac
      421 tggcttcggc tctggaaata ggctgaaaat cttccggaca tggagaatac attgtcttca
      481 tataattaag tcgtctggat ttctcattaa taaactcctc tatttttaat tgtgccatac
      541 ttctggttat accagagata tacttcttgt catatatgtt tggtgattca ttaaagtaac
      601 catgttcaac tctaggataa atatttctca ctatatattc agtatttgca gtatttattg
      661 gaaatacgtt cttaatattc ttgatgggac taatcatttt aatctctata ctcaaatata
      721 aacattacca tgaacaatat aattgttcct aagtggaatg tctccacgga gaacacttta
      781 tctatcggga aatagtgcgg gcaacagtgt ttaattatat cgagtgcaat tacaatcgct
      841 ggcgacggca ccgttggtgt ggcggcctca gtccggaaca atttgaaaac cagaacctcg
      901 cttaggcctg tgtccatatt acgtgggtag gctcacagag cctcacttac gactgcgtcc
      961 atattatgtg ggtaggatca ataaagacgt tttatatgtg tctgcttgca gccagaaaat
     1021 ttataccctg caggtatcat aaacatgctt gataacttaa ttggggctcc ctcttccttt
     1081 tatggtgtaa ttgtttgatt gaatacttcc ggggatttca gtatgaaatg aagtatattt
     1141 aatatacttt acaaaaagtt tgtattagtg gaataaggac aatagcttat ccaaactgcc
     1201 aagcctcttt attataagta agatctggca ctggaatatt ctcaaaagga ctaacatcat
     1261 caccacgacc ttcaaccaac cattttattg catttaaatc ccagaatagt tcagtagtgg
     1321 tcaatttggg aaacgcacaa tattgtacgg ttacgttatc gcaatagagt gaactctcgc
     1381 cctatacggc gttttcaagg attagtcctt aatcggacaa ccacattaaa gcataataaa
     1441 taattaagct ttaataatat actatagata cagttctatt ataaaaaata ctattttagg
     1501 tgatgcatta aattagtaga atatttattc ttttataaaa gcaccaatga attgtccaag
     1561 taaacatgag ataaaatgtg tagaggataa ctgagtttgg tcactttaac tgtattagtc
     1621 gcagtctgac tctcgacttt aaaaggatgg gaattacctg tactgatagt gagaaagctt
     1681 tccattgaaa taagtcaaat aaaagttgat ggcttcagtg ctaaagtgag gtgcccgcta
     1741 gccgtattca ctcgaatcgg aattcaatgt attgcttctg ccatttccat aatatttcac
     1801 gggaaaaaca aaatctgaaa aactatactt aagtgttgta ttgagtcagt tgagatacaa
     1861 atacatattt atcagcacac gttacgtcaa atacaggagg ttgtcatgtt ggtactgaaa
     1921 ggcacgttgg ctattgcagc cgtgatgaca atttattgtc ttatcgttgt tcttatggaa
     1981 cgcttttctg attgatttca tattggcgag agaacaagaa ggtgagtaga attgtcgcgg
     2041 aatcctgaaa ctatcagcct caggtatgaa caccaaaagg taggtagaga aatctatggc
     2101 ccccgttact ttattcgcac aatgaggtat ttttttattt ttacaaaagt tcaataaata
     2161 ttataataaa agatatgttc ttggtaacat aaacaaatat ttaattaaaa taatgcttgg
     2221 cattgacgtt tatcttataa agtaagaaga tcatggacat ttcaaccaaa aaagtagagt
     2281 tctcgatgaa attaaaattc tttgtacttg cactttgtgt acctgcgatc tttactacac
     2341 atgctaccac taactatcca cttttcatac cggacaacat cagtacggat attagtctag
     2401 gatctctgag tggcaaaaca aaagaacgcg tttatcatcc caaggaaggg gggagaaaaa
     2461 ttagtcaact ggactggaaa tacagtaatg cgactattgt tagaggtggc atcgattgga
     2521 agctaattcc aaaagtgtct ttcggagttt ccggttggac tactttaggt aaccagaaag
     2581 caagcatggt tgataaagac tggaacaatt ccaatacccc tcaggtatgg accgaccaga
     2641 gctggcatcc caatacgcat ctccgtgatg ctaacgaatt cgagctgaat cttaaaggtt
     2701 ggttattaaa taatttggat tatcgacttg gactaatagc aggttaccaa gagagtcgtt
     2761 acagttttaa tgcaatggga gggagttata tttatagtga aaacgggggg agcaggaata
     2821 aaaaaggggc acatcctagt ggtgaaagaa caataggtta caaacagctc ttcaaaatac
     2881 cttatattgg attaactgct aattaccgcc atgagaattt tgagtttgga gcagaactga
     2941 aatatagtgg ttgggttctt tcatctgata cagataaaca ctatcagact gagacaattt
     3001 ttaaagatga aataaaaaac caaaattact gctctgttgc tgcgaatatt ggatactatg
     3061 tcacccccag tgcaaaattt tatatagaag gctccagaaa ttacatttct aataaaaaag
     3121 gtgatacatc tctttatgag caaagtacca atatatctgg caccattaaa aatagtgcaa
     3181 gtattgaata tattggtttt ctcacttccg caggtataaa gtatattttt tgaattggtt
     3241 caccgagttg attacgtttt catggctaaa aattgccgcc cattgtgagt catattcgcc
     3301 ctgactttcc agaaccatac gaactgcccg ttgacggact tcgggggaaa aacgtgtatt
     3361 tttagtcatt ctgtttacct ctttctcagg gcgtttagtc tccaggattt ccggggcggt
     3421 tcaaaaaact cttcgttgat tactgtggac ttactgttgg cattaccgac cctgagaccg
     3481 gagaaataag aaccgcttag atcatcgtgg ctgttctcgg ggtatcaagt tacacatggg
     3541 cagaggctac ccaaggtcta ctattatcgc ctgaaagcgc tgatggaaca gtgctatcag
     3601 ggacatgctg atgaaaatac ctaaataatc agccagcact ctatctttcc aaatccacag
     3661 catagcaaag agagcaaaag agcctgtaaa ttcagaaatt aaggaaacgc ctcttggtgt
     3721 acataatcta tcttatgtta aatgaaatct aatttcttga ttatatgctg gatcgttcgt
     3781 gggtggcttt gcccctatat ttccagacac ctgttatcat tggcccgctg ccgcagatat
     3841 tcccgtggcg agcgataatc cagcgcacta tgcggatgcc attcgttata atgctcgaat
     3901 gcctctgcaa ggttctttgc tgccgttaac ccgtcaggtt tgggcatgat actgatgtag
     3961 tcacacttta ttgttttcac gaagctcttt gctattctgt tactctccgg actccgcacc
     4021 gccgtgttct tcggttcaac aaagtcccaa catccgggtg aactggcgtg tttcattagc
     4081 tcggtagcat gaaccattat ccgtcagtca cttcactgga gacgccggaa gctcgttgcc
     4141 gaagcggtgt tccaccgctc ccagcatgtc gccaacggga gagcatctac ggtctgattg
     4201 aactgtttat tcaaattgtc caccgtctgt cagtacgctc tgaacgtcgg ctggttaaaa
     4261 cactgctcgc tgattttcag agggttcacg gtaaaacagc acttttattc cggattgcgg
     4321 aagcggctct gaataatccg gatgggctgg ttaaagaggt tgtgtaccat ctgcatattg
     4381 ttgaaccaga tcgcagcggt aagagaagtt catcatactt ggcgcagtta cgcgatgttt
     4441 ctgctagagg tgatgccgta aagaacggca gaacattacc ggaacaagat agcggtctac
     4501 ctgcactggt atcagacccg gggcttcccc ggatgatctc cacggtactt taggtactgt
     4561 gtctatatta tgtaggtagg atcaattaac tttgtagttg aaattctttc attgaaataa
     4621 tgaataaaaa agacaaaccg cattaaacaa tgccagaata taattgcatt tttgttccgt
     4681 ttttttaaat catagtatac aaagaagctt gcatactata tgaaaatata acacccttta
     4741 ctatgatgtt tataaaacaa tatatggagt catgtaggta tgaatttaga tggtgttaga
     4801 ccatactgta gaatagtcaa taaaaagaat gaaagcatat cagatattgc atttgcacat
     4861 ataataaaaa gggtaaaaaa ttcatcatgt actcacccaa aagcagcatt ggttttttta
     4921 ggagagaaag gtttttgtga tagcaatgat gttctatcta ttatgggaca acaaatacca
     4981 agagtattta agaacaagat gttatatgat tatgttttta aaaatgaaaa aagtaaaaat
     5041 gattttctaa aaatggctga atcatggcta ccacagagtg aaccaatagt aataaataat
     5101 gatgatgacg cattgaatgc tgctgcttat ttttctgtaa aaaaagcgaa aataaaaaca
     5161 gtaaacgata ctgattttaa agagtataat aaggtttata ttcttgggca cggtagtcct
     5221 ggttctcatc aattaggcct tggttcggaa cttattgatg tacaaacaat catttcaaga
     5281 atgaaagact gtggtattct aaatgtgaaa gatatccgtt ttacttcatg cggctccgct
     5341 gataaagtgg ctcctaaaaa ttttaacaat gcccctgctg aaagtctttc ttgtatcctt
     5401 aactctctgc ctttttttaa ggaaaaagaa tctttgctag agcagataaa aaaacacctt
     5461 gaaaacgatg agtcattgag tgatggtcta aaaatatccg gctatcatgg atatggagtt
     5521 cactatggtc aagagctttt tccctactca cattatcgtt caacttcaat tcctgctgat
     5581 ccggagcata cagtaaaaag aagctctcag aaaaagactt ttattattaa taaagaactg
     5641 gattagtata aaatttttaa cctatagtta attgtttgca tttaagatga agttatttta
     5701 tctttagtag aaaagtgctt ccctatgagg gaaaatcaca aatcatcata atacagagac
     5761 aaaacgatac gaaaaaatag ataaaaaaca tcattctttt accacattat tttccgtgaa
     5821 tatgaaaaat aatgttatta ctttaatata agactatttt ttgtttttcc atcactctgt
     5881 tcaaattttt ccgcatgact tgtgtttttt gtaatacagc tcgtttttta cagctgacca
     5941 aaatcatcaa ttaattatgc taaggaaata aattatgaaa accaaaaact ttcttctttt
     6001 ttgtattgct acaaatatga tttttatccc ctcagcaaat gctctgaagg cagaaggttt
     6061 tctcactcaa caaacttcac cagacagttt gtcaatactt ccgccgcctc cggcagagaa
     6121 ttcagtagta tttcaggctg acaaagctca ttatgaattc ggccgctcgc tccgggatgc
     6181 taatcgtgta cgtctcgcta gcgaagatgc atactacgag aattttggtc ttgcattttc
     6241 agatgcttat ggcatggata tttcaaggga aaatacccca atcttatatc agttgttaac
     6301 acaagtacta caggatagcc atgattacgc cgtgcgtaac gccaaagaat attataaaag
     6361 agttcgtcca ttcgttattt ataaagacgc aacctgtaca cctgataaag atgagaaaat
     6421 ggctatcact ggctcttatc cctctggtca tgcatccttt ggttgggcag tagcactgat
     6481 acttgcggag attaatcctc aacgtaaagc ggaaatactt cgacgtggat atgagtttgg
     6541 agaaagtcgg gtcatctgcg gtgcgcattg gcaaagcgat gtagaggctg ggcgtttaat
     6601 gggagcatcg gttgttgcag tacttcataa tacacctgaa tttaccaaaa gccttagcga
     6661 agccaaaaaa gagtttgaag aattaaatac tcctaccaat gaactgaccc cataaagctg
     6721 gacagcctgt atcaggctat ggagggccca tagacaaatc taccctatat gagcatagga
     6781 ggagtctatg ggcacaccac gttttacccc tgaatttaag ggattactgg aaaggctggg
     6841 acatatccct ccggcagaag cagaaaaagc ttattatgct gccatcggaa acgatgatct
     6901 ggcaacctga gttcacagat aaaacattct ctaggaaact cggggcggtt ccgttcacca
     6961 catgcaatgt ggtgttgcag gggaacggtc tgcaccatcc cctatgtcga tcaatataac
     7021 agaaatgaca acttcagatt cagggcacaa cctaaatata ttttaggtca cctctcaaat
     7081 cgtttgcctg ataccgctcc attttttaat aaaaaaatca atcatttttg aatctttgct
     7141 tttcattgca ttgtcaagca tagtgtcccc actatttgct ttttgaaatt ttttgtttac
     7201 atcaaccaat ccattattga taaaatattc tagcacttta tagttggcac catctttact
     7261 aagtaagtat tcaacatcat acagattaca atatctattg tttttcattt tctccatctc
     7321 tgcgacatct tgttttgtaa aattaaattt agatagtaaa taaagtgcta tatcagcttt
     7381 tttattgcct aaagaataag caagggcctg atgagccatt tttttatcag tcactttatt
     7441 gttaatctct tcataattag atgctttaat cgcatcctcc aaagatatgt ctctgagttt
     7501 tatttttttg aagttatctg tacttagcat tcttggtata tgatactcaa gctgaaacac
     7561 aaagtttata attctatcca gactaacagg atcaatattt ttgttataac aaaactctct
     7621 gaatccttga tctttactct ttcttataaa atcaataaga tgcaacccaa ttccaagcct
     7681 catatcttta gtgttaaata ttggaacatc agttttcccc tgattaccat gcacatacct
     7741 gctaacttca tccacaacct ctttaaagtt atctaagaaa aatgattggt gctcatggta
     7801 aaaaacaggt ggaatagcat tatcaaagtg atcggttaat gtcatatatc catatggaga
     7861 gtcatgatca atgatata
//
LOCUS       DAJTYM010000146         7733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTYM010000146 DAJTYM010000000
VERSION     DAJTYM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1696
                     /locus_tag="OO575_003630"
     CDS             <1..1696
                     /locus_tag="OO575_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6666435.1"
                     /translation="NLIKSARVGYTKMVLGVIGYFIEHKSRNTLLFQPTDSAAEDFMK
                     SHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSVDV
                     VCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIKGSCQIEKAANESAH
                     FMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEHNGCVIRQSELDQTG
                     GRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFTTRVQIVCDWLDALK
                     DPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVRVVYLTAGIDSQRNR
                     FEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCW
                     DIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAK
                     EILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEELVEKWEKGKMRLLWD
                     NKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNRPLKNWQRSCPEE"
     gene            1697..1900
                     /locus_tag="OO575_003631"
     CDS             1697..1900
                     /locus_tag="OO575_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6666436.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            1897..3490
                     /locus_tag="OO575_003632"
                     /pseudo
     CDS             1897..3490
                     /locus_tag="OO575_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3480..4985
                     /locus_tag="OO575_003633"
     CDS             3480..4985
                     /locus_tag="OO575_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6666437.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5022..5369
                     /locus_tag="OO575_003634"
     CDS             5022..5369
                     /locus_tag="OO575_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6666438.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5427..6455
                     /locus_tag="OO575_003635"
     CDS             5427..6455
                     /locus_tag="OO575_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6666439.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6507..6893
                     /locus_tag="OO575_003636"
     CDS             6507..6893
                     /locus_tag="OO575_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6666440.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            6905..7282
                     /locus_tag="OO575_003637"
     CDS             6905..7282
                     /locus_tag="OO575_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6666441.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7269..>7733
                     /locus_tag="OO575_003638"
     CDS             7269..>7733
                     /locus_tag="OO575_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6666442.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1769 a         1826 c         2417 g         1721 t
ORIGIN      
        1 taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg tgatcgggta
       61 ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact cagctgctga
      121 agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc tgaaaaaact
      181 ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc gtttttcctc
      241 tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg aaaaatctgt
      301 cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa aagaaggttc
      361 gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat ccatacgcgg
      421 ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg aatctgcgca
      481 tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc tgaagtttgg
      541 cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa cggtgtatta
      601 cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga ccggggggcg
      661 ctggatttgt gacaataccg ggatgtggac gtgtgacggt ctgacgtttt tcagtgcttc
      721 gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg cgtacagtcc
      781 gttcaccacc agggtacaga tagtctgtga ctggctggat gcactgaaag atcccaacgg
      841 cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg tgggcgaaaa
      901 actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg tgcctgtccg
      961 ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga tgtatgtctg
     1021 ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca tgggacgtcc
     1081 tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat accgccatgc
     1141 agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga tcgatggcga
     1201 aatcgtgtat cagaggtcaa aaaaacacgg tattttccgg gtactgccgg taaaaggtgc
     1261 ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc ggggtgtgta
     1321 tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa tgaaggccga
     1381 tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata atccggagat
     1441 tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa agtgggaaaa
     1501 aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc tggactgcct
     1561 ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg atctggctgt
     1621 gcagttgcat cccgcaagga tgcaactgca caaccgaccc ttgaagaact ggcagcgaag
     1681 ctgtccggag gagtgaatgg ttacagtcgc tgaactgcag gcgctgcgtc aggcgcgcct
     1741 tgatttatta accggtaaac gggttgtgtc ggtacagaaa gacggtcgca ggatcgaata
     1801 tacggcagcg tccctgaatg agcttaaccg tgcgatcaac gatgcggagt cggttctggg
     1861 tacgacccga cgccgccgac cgctgggagt gaggttatga aacgaacgcc tgtcctgatt
     1921 gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acggaggcgg cgcaggattt
     1981 ggcgggcaaa tggcagagtg gttaccaccg gcgcagagtg ccgatgcggc cctgttgcct
     2041 gcattgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg tatcgcggct
     2101 aatgcggtgg ccctgcataa ggatcatatc gtcgggcaca tgtttcttat cagctaccgt
     2161 ctgaactggc gttggctggg gatgcgggag accacggcaa aaagttttgt cggggaggtg
     2221 gaagcagcct ggtcggaata cgcagaagga atgtttggcg agatcgacgt ggaagggaaa
     2281 cgcacgttta cggaatttat ccgtgaaggg gtgggcgtgc atgcctttaa cggcgaaatc
     2341 tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg ttttaaagcc
     2401 gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccactt tctgcgggcc
     2461 ggggttgagg tcgatcggta tgggcgggcg attgcgtacc atatctgtga ggatgatttt
     2521 ccgttctctg gtgccggacg ctgggagcgg atcccgcgtg agcttcccac cgggcgtccg
     2581 gccatgctgc atatttttga gccggtggag gacgggcaga cccgtggggc taatcagttt
     2641 tacagcgtaa tggagcggat gaagatgctg gactccctgc aggccacgca gcttcagtcg
     2701 gccattgtga aggcgatgta tgcagccaca atcgaaagtg aacttgatac cgaaaaggca
     2761 tttgaatata tcgcgggggc accgcaaggt cagcaggata atccgcttat taatattctg
     2821 gagaagttca cccgctggta tgacacgaat aacgtgacgc tgggcggtgt taaaattccg
     2881 cacctttttt cctggcgatg cgctgaacct gcagacagcg caggattcag acaatggatt
     2941 ttcggcgctt gaacaggcgc tgctgcggta tattgcagcc ggtcttggcg tttcctacga
     3001 acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct ccgccaatga
     3061 gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg ccacgcagat
     3121 gttttcctgc tggctggaag aggcgcttct tcgggggatt attcgtccgc cacgggcgcg
     3181 ttttgatttt taccaggcgc gttcagcctg gtcacgggca gagtggatta gctccggaag
     3241 aatggccatt gacgggctca aggaggtcca ggaatcggtg atgcgcattg aggccggact
     3301 gagcacgtat gagaaagaac tggcactgat gggcgaagat tatcaggaca ttttccgcca
     3361 gcaggtcagg gaatccgcag agcgcgaaaa agccggactc tcacgtccgg tgtggatagc
     3421 ccaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga caacatcacg
     3481 tgagacgtaa tctttcacat attatcgctg cggcattcaa tgagccgctg cttctggagc
     3541 ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgtga gatgggcgca gcaagccttt
     3601 cggtaccgca acagcaggta cagcttgacg ctcccgggat gctggctgaa acggacgagt
     3661 acatggccgg aggcaaacga cctgcccgtg tttaccgggt ggtgaacggt attgctgtac
     3721 tgccggtgac cggcacgctg gtacaccggc tgggtggcat gcggccattt tccggaatga
     3781 ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcggacact caggtacggg
     3841 gcgtactgct ggacattgac agttctggcg ggcaggccac cggcgcgttt gactgcgctg
     3901 acatgattta ccgtctccgg cagctgaagc ctgtctgggc actgtgcaat gacacggcct
     3961 gttcagccgc catgttactg gcatcggcct gctcccgacg gctggttacc cagacatccc
     4021 gtatcggctc cattggggtg atgatgggcc atgtcagcta tgccggtcat ctggcacagg
     4081 ccggtgtgga tatcacgctg atttacgccg gatcgcacaa ggtggatggc aatcagtttg
     4141 aatccctgcc agcaaaggtt cgccaggaca tgcagcagcg cattgatgcg gcgcgccgga
     4201 tgtttgctga aaaagtggcg atgtataccg gtttatctgt ggatgcggtt atgggaacag
     4261 aggccgccgt ttttgaaggt cagtccggta ttgaggccga actggcggat gaattaatca
     4321 atgcgtcgga cgccatcagc gtgatggctg cgacgctgaa cacacacgat acaggaggca
     4381 ctatgccgca attaactgca acggaggctg ccgcgcagga gaaccagcga gtgatgggga
     4441 tcctgacgtg tcaggaagcg aaaggacgtg aacaactggc cacgatgctg gcagggcaac
     4501 agggtatgag cgttgagcag gcccgggcga ttctggccgt ggcggcaccg caacaatctg
     4561 gtgtatctgc gcagagtgaa gccgatcgca ttatggcatg tgaagaggcg aaaggacgtg
     4621 aacaactggc ggcaacgctg gcggcgatgc cggagatgac ggtggaaaaa gcccgtccga
     4681 ttctggctgc ttcaccgcag gcgaatgccg gaccctcact tcgtgatcag attatggccc
     4741 tggatgaggc aaaaggggca gaggcgcagg ctgaaaaact ggcggcctgt ccggggatga
     4801 ccgtggagaa tgcccgggct gtactggcgg cagcctcagg cagggcagaa ccggtttctg
     4861 catccacaac cgccatgttt gaacatttca tggcgaacca ttcaccggca gcggtacagg
     4921 gtggcgtgcc acagacgtca gcagacggtg atgcggacgt gaaaatgctc atggccatgc
     4981 catgaagtca gtgctgacca tcaatatgag gtttttacaa tatggtaacg aaaaccatca
     5041 ctgaacagcg tgcggaagta cgtatttttg ccggtaatga tccggcccat accgccacag
     5101 gctgcagcgg gatttcctcg gcaacaccgg ctctgacgcc cctgatgctg gatgaggata
     5161 ccgggaaact ggtggtctgg gacggacaga aggcaggcca tgtggttggc atactggtac
     5221 tgccgcttga aggtacagag acggtgctga cgtattacaa gtcggggacc tttgcgacgg
     5281 aggccatcca ctggcctgag agagtggata cacacaaaaa ggcgaatgcc tttgccggca
     5341 gtgccctgag tcacgcagcg ctgccgtaac attttatcag gtcactgcgg tagcctgact
     5401 gatttctgaa tgaaaggaac tgatttatgg gattgtttac gacccgccag ttactcggtt
     5461 ataccgaaca aaaagttaaa tttcgtgcgc tgtttctgga gctgtttttc cgccgtacgg
     5521 tgaatttcca taccgaagag gtaatgctgg acaaaattac cggaaaaacg ccggtggcgg
     5581 cctatgtctc cccggttgtt gaaggaaagg tgttgcgtca ccgtggcggt gaaactcgtg
     5641 tactgcgccc gggctacgtt aagccgaaac acgaatttaa ttaccagcag gcggtggagc
     5701 gtcttcccgg tgaagatccg gcccagctga acggcccggc ttaccgtcgc ctgcgtatca
     5761 tcaccgataa cctcaaacag gaagagcacg ccattgtcca ggtggaagag atgcaggcgg
     5821 tgaatgccgt gctgtatggc aaatacacca tggaaggaga gcagtttgaa actgttgagg
     5881 tcgattttgg ccggtcggtg ggaaataaca ttacgcaggc aggagggacg gagtggagcg
     5941 ctcaggaccg cgataccttt gatccgacgc atgatctgga tgcgtactgt gattttgctt
     6001 ccggcaccat caatattgcc attatggatg gtaaggtctg gcgtctgctg aatggcttta
     6061 agctgttccg cgaaaaactg gatacccgtc gtggctcaaa ttcacagctg gaaacggcag
     6121 tgaaagacct gggggcggtg gtgtccttca aagggtatta tggtgatctg gcaatcgtgg
     6181 tggcaaaaac gtcctatgtg gcagaggacg gtaccgaaaa acgttatctg ccggacggca
     6241 cactggtact gggaaatacg gccgcagagg gtattcgttg ctacggtgcc attcaggatg
     6301 cgcaggcatt gtctgaaggc gtggtggcgt cttcccgtta tccgaaacac tggctgaccg
     6361 tgggtgatcc ggcgcgtgaa ttcaccatga cgcagtccgc gccgctgatg gtgctgccgg
     6421 atccggatga gtttgtggtg gtgcaggtga aataatccgt gagcgggagc taaatgctcc
     6481 cgtttttcat tcaatcagga gcgggtatgg ctacgaaaga agaaaacctg aaacgtcttt
     6541 gtgagctggc tgagaggctg gggcgtgaac cggatgtctc cggtagtgcg gcagatattg
     6601 cgcagcgagt ggcggaactg gaggaagagc ttggagatgc gggggaggtc gcagagccgg
     6661 atacgtactc accatcgaaa gatgatccga ccgcgcataa aggcgagtca gttcctgaac
     6721 agccaggcat tgttaccgga gacgaaacgg ggcaggttac ggtggtggca ctggcgacac
     6781 tgcatacaaa atgtaatggt tcagtgatat ttgtttcacc gggaacgtcg tttcgtgttt
     6841 ctgctgaggt ggctgccagc atggcggcag gtggtctggc aaaaaggcag taacaggaga
     6901 tgtcgtggac ggtttcgata atctgtttga tgctgcgctt gccggtgtca atgaagtcat
     6961 tctccgtgat atggggatca gcgccgtcat tacgtccgga gaacttgagg ggacacacct
     7021 gacaggggtt tttgatgatc ccgaaagtat ttcttttgtt gccgggggga tccggcttga
     7081 ggattcctca ccgtgcctgt ttgtgaaaac ggcagatata tcgcagttac gccgtcagga
     7141 tacgttaact atcggggatg attctttctt tgtggatcgt attactccgg atgacggggg
     7201 atgctgttat atccggttaa ggcggggcag tcctgcccca cagaataatg ccagaatgag
     7261 gcgatatgat gaagggactt gagaatgcca tccgcaatct gaacagcctc gatacccgca
     7321 tggtgccaca gaccagcgca tgggcggtaa accgtgtggc ggcaaagatt gtttctgtcg
     7381 ccacacggca ggttgcgcag aataccgttg ccggggataa ccaggtgaaa ggtattcccc
     7441 tgaaaacggt cagggagcgt gtcaggctgc ttaaagccag cccctcagga aaaatgtatg
     7501 cccgcatgcg tgttaaccgg ggtaacctgc ccgccattaa gctgggtacc gcacaggtca
     7561 gactggcccg ctcccggcat ggcagcaact cacgtcatcg tggcagcgta ctgaaagtgg
     7621 ggaaatacct tttccgggat gcgtttattc agcaactggc gaatggtcgc tggcatgtga
     7681 tgcggcgtat tgacggcaaa aatcgttacc ccattgatgt ggtgaaaatc ccg
//
LOCUS       DAJTYM010000147         7732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTYM010000147 DAJTYM010000000
VERSION     DAJTYM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            123..434
                     /locus_tag="OO575_003639"
     CDS             123..434
                     /locus_tag="OO575_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6666443.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            529..1062
                     /gene="ymdB"
                     /locus_tag="OO575_003640"
     CDS             529..1062
                     /gene="ymdB"
                     /locus_tag="OO575_003640"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6666444.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1004..2485
                     /gene="clsC"
                     /locus_tag="OO575_003641"
     CDS             1004..2485
                     /gene="clsC"
                     /locus_tag="OO575_003641"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6666445.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2493..3650)
                     /gene="mdoC"
                     /locus_tag="OO575_003642"
     CDS             complement(2493..3650)
                     /gene="mdoC"
                     /locus_tag="OO575_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6666446.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4044..5579
                     /gene="mdoG"
                     /locus_tag="OO575_003643"
     CDS             4044..5579
                     /gene="mdoG"
                     /locus_tag="OO575_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6666447.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5572..>7732
                     /gene="mdoH"
                     /locus_tag="OO575_003644"
     CDS             5572..>7732
                     /gene="mdoH"
                     /locus_tag="OO575_003644"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6666448.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPE"
BASE COUNT         1953 a         1821 c         2027 g         1931 t
ORIGIN      
        1 aatttgttga aatatcgagc ataagatgaa tctggagaga atggtctgct gcgaatcagc
       61 caacctgaaa gtatggataa cacaaccttc aaggatgact aatcattgag gaaatagaat
      121 aaatgttcag acctttttta gactctctta tgctcggcag tttgtttttt ccttttattg
      181 ccattgcagg aagcaccgcg caagggggcg tgatccattt ttatggccaa attgtggaac
      241 cggcatgtga cgtcagcacc cagtcatcac ccgtagaaat gaactgccca cagaatggtt
      301 ctattccggg caaaacctac tccagcaaag cgttaatgag cggcaatgtc aaaaacgcgc
      361 aaatagcctc agtaaaagtg cagtatcttg ataaacagaa aaagctggct gtaatgaaca
      421 tcgaatataa ctaagtttct gaacaactca cgttgctgag cagaaaaatg cgatttaacc
      481 aaaaagcctg ctgtacactt aagaaacaag aaggtgtaag gaggcgttat gaaaacgcgt
      541 attcatgttg tgcagggtga tattaccaaa ctggccgttg atgtgattgt gaatgcggct
      601 aatccgtcat taatgggagg cggcggcgtc gatggggcca ttcatcgcgc agcgggtccg
      661 gccctgctgg atgcctgttt aaaagtcagg caacagcagg gcgattgccc tacggggcat
      721 gccgttatta cgcttgcagg cgatcttccc gctaaagccg tagtacacac cgtcgggcca
      781 gtctggcgtg gtggggaaca aaacgaagac cagcttttgc aggatgccta tctgaatagc
      841 ctacgactgg tggcggcaaa cagctatacg tcagtggctt ttcctgcaat cagtactggg
      901 gtttatagtt accctcgtgc ggcagcggct gaaatcgcag ttaaaaccgt ttcagaattt
      961 attacccgtc acgctttacc cgaacaggta tactttgtct gttatgatga agaaaacgcc
     1021 cacctctacg aaagactcct tacccaacaa ggagatgaat gatttgcccc ggctggcgag
     1081 cgcggtgctg ccactgtgtt cgcaacatcc cggtcagtgt ggtcttttcc ctctggagaa
     1141 aagtctggat gcgtttgccg cccggtatcg tctggccgaa atggcagagc atacgctgga
     1201 tgttcagtat tacatctggc aggacgatat gtcggggcgg ttactgtttt ccgccctgtt
     1261 agccgcagca aagcgtggcg ttcgcgtccg tttgttgctg gacgacaaca atacgcccgg
     1321 acttgacgac attttgcgct tgcttgacag tcatccacgc attgaagtcc ggctttttaa
     1381 tcctttctcg tttcgcttgc tacgtccgct tggatatatc accgactttt cccgtctcaa
     1441 tcgccggatg cacaataaaa gtttcactgt cgatggcgtg gttaccctag tgggaggacg
     1501 aaatattggt gatgcctatt ttggtgcagg ggaagagcca cttttttcgg atttagatgt
     1561 catggcaata ggccccgtgg tagaggatgt tgccgatgat ttcgcccgct actggtattg
     1621 caaatcggtt tcacccttac agcaggtgct ggatgtcccg gaaggtgaaa tggcggatcg
     1681 catcgagtta cccgcctcct ggcataacga tgccatgacg catcgttatt tacgcaaaat
     1741 ggaatccagt ccatttataa atcatctggt tgatggaacg ttgccgctta tctgggcgaa
     1801 gacacgttta ttaagtgatg atccggcgaa aggggagggc aaggcaaaac ggcattcact
     1861 gttaccgcag cgcctgttcg atatcatggg ctcacccagt gaacgcatcg atattatctc
     1921 ttcctatttt gtaccgacac gcgcaggtgt ggcgcaactc ttacggatgg tgagaaaagg
     1981 ggtaaagatt gcgatcctaa ccaattctct tgccgctaac gatgttgctg tcgtccatgc
     2041 cggatacgcg cgctggcgca aaaaattgct ccgctatggc gtggaattat atgaactcaa
     2101 gccgacgcgt gaacaaagca gtacgttaca cgatcgcggc ataaccggta attccggtgc
     2161 cagcctgcat gctaaaactt ttagcatcga tggtaaaacg gtgtttatcg gctctttcaa
     2221 tttcgatccg cgttcaacat tgctcaatac tgaaatgggc ttcgtgatag agagcgaaac
     2281 gctggcacag ttaattgata aacgctttat tcagagccag tacgatgcgg cctggcagct
     2341 ccgtctggac aggtggggac ggatcaactg ggttgatcgt catgcaaaga aagaggttat
     2401 tctcaaaaaa gaacccgcca ccagtttctg gaagcgggtt atggtcagac tggcgtcgat
     2461 attgcccgtg gaatggttat tgtaaatgtg gcttaacggg ctggtgcttt atcgttctca
     2521 cgcttaacaa ccggtttacc agagaacaaa aacttcagta acgggatgcg taaatgaatt
     2581 tcatacagaa ttatcgcaat ccctactacg aatatcaggc cacagagaaa accaagccag
     2641 ttggaggtga tgtgcggtgt aatgtatgcg ccgaaaaaca gcgttaacgg gtggtgaacc
     2701 agatagataa acagcgatgc gttaacaaaa taagtcaccc gcgctgactg gaagttaagc
     2761 aaacggtggc caaaggagaa gaccacattc accatccaca ggccgaggac catggtgatc
     2821 accgactcgg tttcgtacat ccaggcatcg ccactgccat agcgctggtt gagtaaataa
     2881 gcgacaaacg ccaatgctgc agcgagggta cagccacgag acggcgtggt aaacaaggct
     2941 ttaagatgag ggaaaatgaa agccagtgcg ccgaggataa agaacggtaa ataaaatagc
     3001 gtttgcatga caataaaatt gaacatgcca ttactcagaa tgggtggata cacaataaaa
     3061 atcgttcttc ttattaccgc atagccgatg ccgaggcata aaaaaatcac cgatagtttt
     3121 accatcgaga attttttatt cgttttatca gaattttcta aattatttct gatgcgctta
     3181 aatatccata cacacagcgt cgtcatgact actaacacca gtaaaaacca caggtgtgat
     3241 attaattccc aggccaacgt attatatttg tcatacaatg atagcccagg ccaactttcc
     3301 gctttccctt tgacatattg cagcataata aattgcggta atgtcagtag ggggatggct
     3361 gttaacatcg ggatacctac acgttcgaca cgtactttcc accatttttt caagggatag
     3421 cgtaaaaaaa gcatgtagga aaagtatccg gatataacga aaaatacctg catgcggaac
     3481 gagtggatga agtcattaaa aagggttagc cacaatgacg gttcggcgct attcacatgc
     3541 catgtatggc tcgaatagat taaagaaata tgaaaaggga tccctaacaa catcagccag
     3601 gcgcggatgg agtcgaggaa atattcacgt tgcgcgggta ctggattcat atatggttaa
     3661 ctaatctcgg atttttcgtc ttatccctgt cgggttatgc ctttaggctt gttgccatag
     3721 cgacaccgac ctgaccgcgc caggcgcagg cttcaaggtt tttatgcata gcatcatcgc
     3781 taccactaac cagaatggaa gcgtctgtaa gacggttgat aaataaattt gctggcaaac
     3841 cctacacgaa gtcgatgctt ctgtctttag gagaagcacg gaaagtgaaa acggttgcaa
     3901 tcaggtgctt aatccatgag ccagcgtgct gaacgatacc gggattcagt tgtcggaatg
     3961 gctggttatc cattaaaata gatcggatcg atataagcac acaaaggggg aagtgcttac
     4021 taattatgaa acataaacta caaatgatga aaatgcgttg gttgagtgct gcagtaatgt
     4081 taaccctgta tacatcttca agctgggctt tcagtattga tgatgtcgca aagcaagctc
     4141 aatccttagc cgggaaaggc tatgaggcgc ctaaaagcaa cttgccctcc gttttccgcg
     4201 acatgaaata cgcggactat cagcagatcc agtttaatca tgacaaagcg tactggaaca
     4261 atctgaagac cccattcaaa ctcgagttct accatcaggg tatgtacttc gataccccgg
     4321 tcaaaataaa tgaagtgact gccaccgcag tcaaacgaat caaatacagc ccggattatt
     4381 tcactttcgg cgatgttcag catgacaaag acacggtaaa agaccttggt tttgccggtt
     4441 ttaaagtgct ttacccgatc aacagcaaag ataaaaacga tgaaatcgtc agcatgctcg
     4501 gggccagcta tttccgcgtg attggtgcag gtcaggttta tggcctttct gcccgcggcc
     4561 tagcaattga taccgctttg ccatcgggtg aagaatttcc acgcttcaaa gagttctgga
     4621 tcgagcgtcc aaaaccgact gataaacgtt taaccattta tgcattgctt gactcgccgc
     4681 gtgcgacagg tgcttacaaa ttcgtggtta tgccagggcg tgacacggtt gtggatgtgc
     4741 agtcgaaaat ctatctgcgc gataaagtcg gcaaactggg ggttgcaccg ttaaccagta
     4801 tgttcctgtt tgggccgaac caaccgtcgc ctgcaaataa ctatcgtccg gagttgcacg
     4861 actctaacgg tctgtctatc catgctggta atggcgaatg gatctggcgt ccgttgaata
     4921 acccgaaaca tttagcggtc agcagcttct ccatggaaaa cccgcgaggc tttggtctgt
     4981 tgcaacgagg tcgtgatttc tcccgctttg aagatctcga tgatcgttac gatctccgtc
     5041 caagcgcatg ggtgactccg aaaggggagt ggggcaaagg cagcgttgag ctggtggaaa
     5101 ttccaaccaa cgatgaaacc aacgataaca tcgtcgctta ctggacgccg gatcagctgc
     5161 cggagccggg taaagagatg aactttaaat acaccatcac cttcagccgt gatgaagaca
     5221 aactgcatgc gccagataac gcatgggtgc aacaaacgcg tcgttcaacg ggggatgtga
     5281 agcagtcgaa cctgattcgc cagcctgacg gtactatcgc ctttgtggtc gattttaccg
     5341 gcgcagagat gaaaaaactg ccagaggata ccccggtcac agcgcaaacc agcattggtg
     5401 ataatggtga gatagttgaa agcacggtgc gctataaccc ggttaccaaa ggctggcgtc
     5461 tggtgatgcg tgtgaaagtg aaagatgcca agaaaatcac tgaaatgcgt gctgcgctgg
     5521 tgaatgccga tcagacgttg agtgaaacct ggagctacca gttacctgcc aatgaataag
     5581 acaactgagt acattgacgc aatgcccatc gccgcaagcg agaaagcagc attgccgaag
     5641 actgatatcc gcgccgttca tcaggcgctg gatgccgaac accgcacctg ggcgcgggag
     5701 gatgattccc cgcaaggctc ggtaaaggcg cgtctggaac aagcctggcc agattcactt
     5761 gctgatggac agttaattaa agacgacgaa gggcgcgatc agcttaaggc gatgccagaa
     5821 gcaaaacgct cctcgatgtt tcccgacccg tggcgtacca acccggtagg ccgtttctgg
     5881 gatcgcctgc gtggacgcga tgtcacgccg cgctatctgg ctcgtttgac caaagaagag
     5941 caggagagcg agcaaaagtg gcgtaccgtt ggtaccatcc gccgttacat tctgttgatc
     6001 ctgacgctcg cgcaaactgt tgtcgcgacc tggtatatga agaccattct tccttatcag
     6061 ggttgggcgc tgattaatcc tatggatatg gttggtcagg atttgtgggt ttcctttatg
     6121 cagcttctgc cttatatgct gcaaaccggt atcctgatcc tttttgcggt actgttctgt
     6181 tgggtgtccg ccggattctg gacggcgttg atgggcttcc tgcaactgct tattggtcgc
     6241 gataaataca gtatatctgc gtcaacagtt ggcgatgaac cattaaaccc ggagcatcgc
     6301 acggcgttga tcatgcctat ctgtaacgaa gacgtgaacc gtgtttttgc tggcttgcgt
     6361 gcaacgtggg aatcagtaaa agccaccggg aatgccaaac actttgatgt ctacattctg
     6421 agtgacagtt ataacccgga tatctgcgtc gcagagcaaa aagcctggat ggagcttatc
     6481 gctgaagtcg gtggcgaagg tcagattttc tatcgccgcc gccgtcgccg cgtgaagcgt
     6541 aaaagcggta atatcgatga cttctgccgt cgctggggca gccagtacag ctacatggtg
     6601 gtgctggatg ctgactcggt aatgaccggt gattgtttat gcggcctggt gcgcttgatg
     6661 gaagccaacc cgaacgccgg gatcattcag tcgtcgccga aagcgtccgg tatggatacg
     6721 ctgtatgcgc gctgtcagca gttcgcgacc cgcgtgtatg ggccactgtt tacagccggt
     6781 ttgcacttct ggcaacttgg cgagtcgcac tactggggac ataacgcgat tatccgcgtg
     6841 aaaccattta tcgagcactg cgcactggct ccgctgccgg gcgaaggttc ctttgccggt
     6901 tcaatcctgt cacatgactt cgtagaagcg gcgttgatgc gccgtgcagg ttggggggtc
     6961 tggattgctt acgatctccc gggttcttat gaagaattgc cgcctaactt gcttgatgag
     7021 ctaaaacgtg accgccgctg gtgccacggt aacctgatga acttccgtct gttcctggtt
     7081 aagggtatgc acccggttca ccgtgcggtg ttcctgacgg gcgtgatgtc ttatctctcc
     7141 gctccgctgt ggtttatgtt cctcgcgctc tctactgcat tgcaggtagt gcatgcgttg
     7201 accgaaccgc aatacttcct gcaaccacgg cagttgttcc cggtgtggcc gcagtggcgt
     7261 cctgagctgg cgattgcact ttttgcttcg accatggtgc tgttgttcct gccgaagtta
     7321 ttgagcattt tgcttatctg gtgcaaagga acgaaagaat acggcggctt ctggcgcgtt
     7381 acattatcgt tgctgctgga agtgctgttt tccgtgctgc tggctccggt acgcatgctg
     7441 ttccatacgg tcttcgttgt cagcgcgttc cttggctggg aagtggtgtg gaattcaccg
     7501 cagcgtgatg atgactccac ttcatggggc gaagcgttca aacgccacgg ctcacagctg
     7561 ctgttggggt tagtgtgggc tgttgggatg gcgtggctgg atctgcgttt cctgttctgg
     7621 ttggcaccga ttgtcttctc gttgatcctg tcaccgtttg tttcggtgat ttccagccgt
     7681 gccaccgttg gtctgcgcac caaacgctgg aaactgttcc tgatcccgga ag
//
LOCUS       DAJTYM010000148         7574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTYM010000148 DAJTYM010000000
VERSION     DAJTYM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..480
                     /locus_tag="OO575_003645"
     CDS             <1..480
                     /locus_tag="OO575_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666449.1"
                     /translation="LEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGE
                     AAKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFP
                     MTFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            452..886
                     /locus_tag="OO575_003646"
     CDS             452..886
                     /locus_tag="OO575_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6666450.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1659..2708)
                     /locus_tag="OO575_003647"
     CDS             complement(1659..2708)
                     /locus_tag="OO575_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6666451.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3159..4349
                     /locus_tag="OO575_003648"
     CDS             3159..4349
                     /locus_tag="OO575_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR6666452.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4739..6199)
                     /gene="gtrII"
                     /locus_tag="OO575_003649"
     CDS             complement(4739..6199)
                     /gene="gtrII"
                     /locus_tag="OO575_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR6666453.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6196..7125)
                     /locus_tag="OO575_003650"
     CDS             complement(6196..7125)
                     /locus_tag="OO575_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6666454.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7122..7484)
                     /locus_tag="OO575_003651"
     CDS             complement(7122..7484)
                     /locus_tag="OO575_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6666455.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         2374 a         1426 c         1505 g         2269 t
ORIGIN      
        1 ctggagaagg ggcggcacga tcagttgctt accgcacttc gcgcgctgct gttaagccgc
       61 aagaatccgt ttggcgatat caaatcggat ggcacggtga aaacggctct cgaaaacctt
      121 ggtttgggag aagcagctaa aaggaatgta ggtacagggg cgaatcagat acctgatatg
      181 agcctgttcg cgtcaattaa taccgtaacg gctgctgcgc aaaaatttcc gtctggatta
      241 attttacagt gcggtcagtt gaatggtgcc ccgaatgtat cttcaacata cgggatgagg
      301 ttcccgatga cgttctcaag agtaattgct gtcgtagtta cattgaacgt tactggcgcg
      361 gctgggcagc cgactgtatc ggcgacaagt gtccagaaca ctggatttaa tattactgtg
      421 tcgcccggtt caggatacgg ttcatctgct gatgcgtatt acattgcaat gggatattaa
      481 caaaatgtca tatttttatt ctgcatcgac aaacggattt tattcgactg aatttcacgg
      541 caccaatatt cctgatgatg cagtggaaat ctcggaatca gagtggaaaa cactgattaa
      601 tgcacagagt gtaacaaaaa tgattacctg tggtgagaac ggtcatcctg tcattgttga
      661 ccgtccttct ccaacaccag aacaattagc cttaataaat gatgaaaaga aatctgcact
      721 gatagcagag gcaacgaatg taatagctcc gcttcaggat gcggttgatt taggtatggc
      781 aacagatgat gaaacgaaac tgttactggc atggaaaaaa tatcgggtgc tgcttatgcg
      841 tgttaatgta gtaaaacccg agtggcctat gcatccaaat aaatagactt gttaatctag
      901 gataattgtt ggaataatac tcgtttttca gtaggttcag gtagcctggg tataatgctc
      961 acttctacaa tgatttcaac ttattttatt taattgatat tacgaaactt aactcataca
     1021 taaacaatat gactcaatac tgtcctaaat agctgcgcag aaaagtgctc cactttcgct
     1081 cagcccttgc tgggcgaagc atcaggaagg cgttctcagc taggttgtgt tatatctggc
     1141 ttgagttttg ttgcccatac gcaacacatt acccatctgt aaacgacaag tgtggcagaa
     1201 ttaggtgcag ctatttagac tatatcgacg cagtaacgat aatagatatc gaaactgtac
     1261 tagatattag ctacttatgg ttaccggggg gagtcttttg agcactggtg ttatcaattg
     1321 gcttcaaata attcgccaat aaattatatt gcgatagctc atcaccttcc aggttgttta
     1381 tagccagttt atcagcgtca ccacctactg tttatgcttt tatttctttg attaataagt
     1441 atatgatatt ttaattcatc tggtgtattt ctttctcatg tgttggtcga ctgaatatct
     1501 gttcagtgaa ggggtaattt tatgtttgca cttcgaacat tcccttcagt tgccaggaaa
     1561 gaaaaccgta acatttgcgc attaatttta acagtataat ctgagcataa atatgcgcta
     1621 tatatctata aaaaggcgca attaattgcg cctttttatc attttatcaa aaatggccca
     1681 ccgactgtcc tcgatagcca atggccaatt ttatctgata catatacaca aatacaggta
     1741 tatatgattg cgcagatagg atttggaata gtcacgctga agcctataat tgctaaagcc
     1801 atagggataa ataatttggt gatatactcc attccacata tgttaagagt atttcttcca
     1861 gctcttacaa taaagtcatt tccacaaatt atttttgcta tgaaaatatt aaagattatc
     1921 aagcctaccg tggtgataat agtattaata gcttgaatat aactacttga tattatgtct
     1981 gcattgaata ttgagataat cttaaaaagt aaaggtttct ggttaagtag ttgatacgca
     2041 ctcattatag cggtaattga gaagacaatg aaaccaattt tagattttcc aaaaaacctg
     2101 tcacgtgtta attctaaaaa catacatcta ccgagaggca ataaccacca gtatgccata
     2161 gcggagtcta tgttccaaaa ccattgagga tctagtaatg ggttatgatt gagcactgtt
     2221 tgtgacaaca tgaacgataa cagtgatatg attaaaatca ctatattgtt cttgactatt
     2281 tctctaaaga ttgcatcaat tgctattata acaaaaaggc agtttataaa ccatattgtt
     2341 cctacgaatt ggttatttct aacaccatat attatatcat aaatgtggtt gtatatataa
     2401 tcaatcgtct cacctgagtt aattgtgttt attattaata tagaaaatgc aaaagtgaaa
     2461 tatggaatca ttaatctgta aaactttgac ttgatataat cgaaaacaga taagtcattt
     2521 tttttgatag taaaaaaacc agcagcgaaa aagaatagtg ggacgtgata actaaaaaca
     2581 aaaggataca atttgccagc ccccaaccca aggtgaccta aatatatagc aaaaataccg
     2641 agaaatttta aagcatcaac ccaatcatgt cttatttttc tgttgctgtc gattttgtta
     2701 ttatacattt aaagtaggct ctccagtttt gtgtgatttt taaacactca ttgcaaatat
     2761 gaatgcctga aagtttatga ttattaatca acgagttcaa tattaattta ttatttttca
     2821 aagtcgcact atataggcat ttacttccgc agtaccaccg atagtatcga tatcaaatca
     2881 gcctgcggcc gcgataactt tcgtaacatt ctgccagcgg ctttgttgaa taaatcaggt
     2941 tcaagataat cgtctctgca tcaggttaga gtttcaggca atacatcctc tttctggcat
     3001 acctgccact ctcgcaccat ggtcatagcc tttacgatct gcatactcac cagaccagag
     3061 cccgctccct tttaggctga ggaatcccca gaaaagaaga caggcataga gttttgtgat
     3121 ctactgattg cgctaacaaa ttcaatgaga tcacctctat gcctgcccgt aaagtatgcc
     3181 agactttttt ccgtaatgct ttagcttcca cgcatcagta tcgacaaaat gccattattg
     3241 attctgctgc tgctttagtc ggtggcgcat ctctttctct taccagcatt ggccggcatt
     3301 tacccggtcc tgctcgcgtg aaagataaaa taaaacgcgt tgactgtctg ttgggtaata
     3361 aacgacttca taatgatatc cctctgatat tcaaaaatat tacatccatg atgacaaata
     3421 aactgtcgtg gtgtgttatc gctgttgact ggagcggtta cccttttcag gaatatcatg
     3481 tccttcgtgc cagcctcctc tgcgacggtc gctctatccc cctgatgagt caggtcttcc
     3541 cgtcaaaaaa aaataacgag gcggtggaaa tcgcttttct ggatgcgctt tccggggcca
     3601 tttctccccg gactcgtgtt gttattgtta ctgatgctgg ttttcaaagt gcatggttcc
     3661 gtcatatcaa atcacaggga tgggatttta ttggtcgtat caggggggta gtgaagttca
     3721 ggctggatag tgacaaagat aagtggctgg acataaaaat gtgcaggggg agttcagagg
     3781 caaaatacct ggggacggga accctggccc ggaaaaaacg ctcgcagtgt gaaggacatt
     3841 tttatcttta taaacattca cctaaaggca gaaaaagcag gcgtgccaga gggaggccgg
     3901 gcttaccaac aacggaaaaa gaacaaaaag ccgcaggcag agaaccatgg ttaatattca
     3961 gcaatacagc tgagtttaat gccaaaaaaa tcatgaaact gtacagtcga agaatgcaga
     4021 tagagcaaaa ctttcgggat gaaaaaagtg aacgctttgg ttttggcctt cgggcgagca
     4081 gaagtcggag gggagagcgt tttctggtat taagtctact ggttacactg gccagtatcg
     4141 tactctggct acttggttat cattttgaaa ataaaggatt tcatttaaag tatcaggcta
     4201 atagtctcaa aaaacgaaga gtattgtctt ttctgacgct ggctgaaaac gtcctgagat
     4261 ttaaccctga acttctacga cgagcacaac ctgaaaagat attgtgccgg ttggccagca
     4321 cttaccgaag tatggtgctg gcttactgat gaaaattttc tggggatccc tcagcctttc
     4381 ggggaggaat gagggctctg atattctttt gccgtagttc atcgtgaaac cgtcggatgt
     4441 catttggcct gagtgaccca tgcctcccaa aagcagagtc gctagtgtta tgtaattagg
     4501 cggttaaact tcaattctca gtgttaagtt tgggtaaacg cttgtaaatg gtattgctaa
     4561 ttttttaatt gactaatatt agaaacggtt agtgtgattg tatatagata tttgagtgta
     4621 aggtatttaa aggggcattc agcccctccg tctatttgtg attgcatcca ttgatgtgtg
     4681 tattattttg agcttgaaaa agggaggcgc tctttaataa taaataaatc tatcaaagct
     4741 aaatattaaa tggaagccac ccttttttct ctgatagttt tattatcacc aaaccattta
     4801 tttcagctat cgaatctgga ttcggccagg aaggtaaact cttcacaatt ggataaatct
     4861 tctctatttg ttctttattt gctggtttgc aattacagat tccatagtaa ttcataaatg
     4921 caaccatcct aaaatagttg ccaccatccc accaaaaaaa agattttcca aaagtgtctg
     4981 cgctcatgga gagcatattt cctgagtcgt ttgaaccata tatatatata tattttccgt
     5041 taagggaaat gccttttgtt tgagatgtgt atgatatttc tttagctaaa gagatatctt
     5101 tctgtcttgc gagagtatca ttcaaaaata gatttttgga tgttgaaacg ccattaaata
     5161 taataaataa aaaaaacatg cagtttaggt attttataga gcgaaaattg cttaagcaag
     5221 aaaacaaaat tactgctact ataggcatta aaacaaacag gcgaggtggc gcccctgagc
     5281 caacaacaac gataaatata accggaagta tataaatgaa aatgattgat gaaatcaaat
     5341 aaattgatct tgtttttaat ttatatgtta gggtaaacat cagaattaat aaaggaatca
     5401 gtaaccactt gtataggttt aaaccagtgg gaggattgtt atagaagtta tatatattat
     5461 ctatggctga tttaacaagc catttaatat cgcttgcatt ttgtatataa tttgagatgt
     5521 actcgttcga ttcaagcgaa aaataatgct tgatagcttt ggttaatagc aggtaaatta
     5581 aggtagataa agctatgagg gatagagata aacatgaact ataaaaagat tcacgaatat
     5641 ttttctcatt tcgtattatc gaatttatct gcaacccaat gacgaatgca ataatatatg
     5701 ttacgaatgt ttggtagatt gccattgaaa gaatggacag tactatacca gaaaatatca
     5761 caatcctatt tttttgcgag tgaaaaataa ttgctgatat cgctgccagt agaaaagcaa
     5821 ttcccacagt atcagcttgg ttaagaaact ctaattgata ggagatctga gggaaggtaa
     5881 taaatactaa catacctatc aataattcat aagattctag ctttagcgat ctgcagatta
     5941 taaatgctga aatgatgata aaagataagg ctattaatgg tgttatataa agtgaaaaag
     6001 gctcagggag aaagtaatgt cgcaaaaaag tgtgaaacca ccgcccaagc gtgattgttt
     6061 gataaaaatt atttgtaaac tcgccatcaa tattaagtgt tccatcgtac aaccaccccc
     6121 aataacaata aattgatatt gcaaaacatg atatgaaggc aaggagattt gcatttttaa
     6181 ataaattaat tttaatcatg ggttacctcg atgagatttt tttatgatgt atttgggtct
     6241 attttttact tcaatataga ttctacctat atattctccg agaacaccaa tcccgatcag
     6301 ttgcactcca cccaagaaaa gtattgatac aagcagggag ggatacccgc gtactgggtt
     6361 accaaagaca agggtgtcta taatcatcca tgcaccatat aaaaatgaaa tgcttgcaac
     6421 aaacaagcct atataagtcc atacgcgaag aggaaaggtt gaaaaacttg tgataccttc
     6481 cagtgccaga ttccataatt tccagccatt aaattttgag atgccagcaa cacgctctgc
     6541 gcgtacatat tcaacgacat ccgtcggacc acccacccag ctcagtatgc ctttcatgaa
     6601 aagattgcgc tcaggcaaca gtttaatgtt ctccacaacc tcacgagaca tgagtcgaaa
     6661 atctccgaca ttttcctcga tctttggggt gcttattttg ttgtgtaatt tatagaacca
     6721 ctcagctgtc ttacgtttca gtcgtccatc agttgagcgg tctgaacgtt tagcaagcac
     6781 catgtcagca cctgcctgcc acttttcaat aagatgaggg ataacctcaa ttgggtcttg
     6841 caggtcgaca tcaataggaa ttacagcatc gccgcttgca tggtctaacc ctgcaaataa
     6901 ggctggttct ttaccaaagt tgcgtgtaaa tgacagcgga acaactagcg ggtctgaaac
     6961 agccagcgcg ttaataattg actctgtggc atctttactt ccgtcattta tgaatacaat
     7021 ttctacttca tatggcttca actcttggaa ttcacgtacc gttttataga aaacaggtat
     7081 cgcttcttct tcattgaaga caggaacgac aagagatatt ttcatttcgc atccctaaag
     7141 acaatgaact ttgaatagac gaaaccgcac accagactga tggcggagaa ggtgataaga
     7201 gtgactattg gaggaagtga gcatttatca gctgcccaac caacaatcac actcaatatt
     7261 cccataaatc ccacgtataa catgtagcgc atcgctgtag tcaatgcttt gaatgtgaat
     7321 cttgcattcg cgaagaagct aaagctcaca gccactacga aacctgtgaa gtttgccaga
     7381 gcctgactgg tatgtgcggc atagatacat acaccaaaaa ccacccagtg tataagtgtg
     7441 ttcagcacac ctatagatgt gtactttaca aataacttta acatttattt aatcaatgag
     7501 ctctgaaagg catgaagtct atcatccaag tctcaattga tcgatacttg ctatgtctga
     7561 tgagacaaaa ctga
//
LOCUS       DAJTYM010000149         7503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTYM010000149 DAJTYM010000000
VERSION     DAJTYM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1807
                     /gene="frdA"
                     /locus_tag="OO575_003652"
     CDS             <1..1807
                     /gene="frdA"
                     /locus_tag="OO575_003652"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6666456.1"
                     /translation="QTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRSH
                     TVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGCP
                     WSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLDI
                     LVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMALS
                     HGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGE
                     PKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICEL
                     AKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLG
                     SNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAK
                     IRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIEL
                     SHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYS
                     DVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            1800..2534
                     /gene="frdB"
                     /locus_tag="OO575_003653"
     CDS             1800..2534
                     /gene="frdB"
                     /locus_tag="OO575_003653"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6666457.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2545..2940
                     /gene="frdC"
                     /locus_tag="OO575_003654"
     CDS             2545..2940
                     /gene="frdC"
                     /locus_tag="OO575_003654"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6666458.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2951..3310
                     /gene="frdD"
                     /locus_tag="OO575_003655"
     CDS             2951..3310
                     /gene="frdD"
                     /locus_tag="OO575_003655"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6666459.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            3373..4506
                     /locus_tag="OO575_003656"
     CDS             3373..4506
                     /locus_tag="OO575_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6666460.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4595..5128
                     /gene="blc"
                     /locus_tag="OO575_003657"
     CDS             4595..5128
                     /gene="blc"
                     /locus_tag="OO575_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6666461.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5125..5442)
                     /gene="sugE"
                     /locus_tag="OO575_003658"
     CDS             complement(5125..5442)
                     /gene="sugE"
                     /locus_tag="OO575_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6666462.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5618..5764)
                     /gene="ecnB"
                     /locus_tag="OO575_003659"
     CDS             complement(5618..5764)
                     /gene="ecnB"
                     /locus_tag="OO575_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6666463.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(5875..6000)
                     /gene="ecnA"
                     /locus_tag="OO575_003660"
     CDS             complement(5875..6000)
                     /gene="ecnA"
                     /locus_tag="OO575_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6666464.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(6052..6618)
                     /gene="efp"
                     /locus_tag="OO575_003661"
     CDS             complement(6052..6618)
                     /gene="efp"
                     /locus_tag="OO575_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6666465.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6660..>7503
                     /gene="epmB"
                     /locus_tag="OO575_003662"
     CDS             6660..>7503
                     /gene="epmB"
                     /locus_tag="OO575_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6666466.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMP"
BASE COUNT         1838 a         1962 c         1974 g         1729 t
ORIGIN      
        1 gcaaaccttt caagccgatc ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc
       61 aattgctgcc gcgcaggcaa atccaaatgc aaaaatcgca ctaatctcaa aagtataccc
      121 gatgcgtagc cataccgttg ctgcagaagg gggctccgcc gctgtcgcgc aggatcatga
      181 cagcttcgaa tatcactttc acgatacagt agcgggtggc gactggttgt gtgagcagga
      241 tgtcgtggat tatttcgtcc accactgccc aaccgaaatg acccaactgg aactgtgggg
      301 atgcccatgg agccgtcgcc cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa
      361 aatcgagcgc acctggttcg ccgccgataa gaccggcttc catatgctgc acacgctgtt
      421 ccagacctct ctgcaattcc cgcagatcca gcgttttgac gaacatttcg tgctggatat
      481 tctggttgat gatggtcatg ttcgcggcct ggtagcaata aacatgatgg aaggcacgtt
      541 ggtgcagatc cgtgctaacg cggtcgttat ggctaccggc ggtgcgggtc gcgtttatcg
      601 ttacaacacc aacggcggca tcgttaccgg tgacggtatg ggtatggcgc taagccacgg
      661 cgttccgctg cgtgacatgg aattcgttca gtatcaccca accggtctgc caggttccgg
      721 tatcctgatg accgaaggct gccgtggtga aggcggtatt ctggtcaaca aaaatggcta
      781 ccgttatctg caagattacg gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa
      841 atatatggaa ctgggtccac gcgacaaagt ttctcaggcc ttctggcacg aatggcgtaa
      901 aggcaacacc atctccacgc cgcgtggtga tgtggtttat ctcgacctgc gtcacctcgg
      961 cgagaaaaaa ctgcatgaac gtctgccgtt catctgcgaa ctggcgaaag cgtacgttgg
     1021 cgtcgatccg gttaaagaac cgattccggt acgtccgact gcacactaca ccatgggcgg
     1081 tatcgaaacc gatcagaact gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg
     1141 ttcctctgtt ggtctgcacg gtgcaaaccg tctgggctcc aactccctgg cggaactggt
     1201 ggtcttcggc cgtctggccg gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg
     1261 caacgaagcg gcaattgaag cgcaggcagc tggcgttgaa caacgtctga aagatctagt
     1321 taaccaggat ggcggcgaaa actgggcgaa gatccgcgac gaaatgggcc tggcaatgga
     1381 agaaggttgc ggtatctacc gtacgccgga actgatgcag aaaaccatcg acaagctggc
     1441 agagctgcag gaacgcttca agcgcgtgcg catcaccgac acctccagcg tgttcaacac
     1501 cgacctgctc tacaccattg aactgagcca cggtctgaac gttgctgaat gtatggcgca
     1561 ctccgcaatg gcacgtaaag agtcccgcgg cgcacaccag cgtctggacg aaggttgcac
     1621 cgagcgtgac gacgtcaact ttctcaaaca caccctcgcc ttccgcgatg ctgatggcac
     1681 gactcgcctg gagtacagcg acgtgaagat tactacgctg ccgccagcta aacgcgttta
     1741 cggtggcgaa gcggatgcag ccgataaggc ggaagcagcc aataataagg agaaggcgaa
     1801 tggctgagat gaaaaacctg aaaattgagg tggtgcgcta taacccggaa gtcgataccg
     1861 caccgcatag cgcattctat gaagtgcctt atgacgcaac tacctcatta ctggatgcgc
     1921 tgggctacat caaagacaac ctggcaccgg acctgagtta ccgctggtcc tgccgtatgg
     1981 cgatttgtgg ttcctgcggc atgatggtta acaacgtgcc aaaactggca tgtaaaacct
     2041 tcctgcgtga ttacaccgac ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac
     2101 gcgatctggt ggtcgatatg acccacttca tcgaaagtct ggaagcgatc aaaccgtaca
     2161 tcatcggcaa ctcccgcacc gcggatcagg gtactaacat ccagaccccg gcgcagatgg
     2221 caaagtatca ccagttctcc ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc
     2281 agtttggcct gaacccagag ttcatcggtc cggctgccat tacgctggcg catcgttata
     2341 acgaagatag ccgcgaccac ggtaagaagg agcgtatggc gcagttgaac agccagaacg
     2401 gtgtatggag ctgtactttc gtgggctatt gctccgaagt ctgcccgaaa cacgtcgatc
     2461 cggctgcggc cattcagcag ggcaaagtag aaagttcgaa agactttctt atcgcgaccc
     2521 tgaaaccacg ctaaggagtg caacatgacg actaaacgta aaccgtatgt acggccaatg
     2581 acgtccacct ggtggaaaaa attgccgttt tatcgctttt acatgctgcg cgaaggcacg
     2641 gcggttccgg ctgtgtggtt cagcattgaa ctgattttcg cgctgtttgc cctgaaaaat
     2701 ggcccggaag cctgggcggg attcgtcgac tttttacaaa acccggttat cgtgatcatt
     2761 aacctgatca ctctgacggc agctctgctg cacaccaaaa cctggtttga actggcaccg
     2821 aaagcggcca atatcattgt aaaagacgaa aaaatgggac cagagccaat tatcaaaagt
     2881 ctctgggcgg taactgtggt tgccaccatc gtaatcctgt ttgttgccct gtactggtaa
     2941 ggagcctgag atgattaatc caaatccaaa gcgttctgac gaaccggtat tctggggcct
     3001 cttcggggcc ggtggtatgt ggagcgcaat tattgcgccg gtgatgatcc tgctggtggg
     3061 tattctgctg ccactggggc tgtttccggg tgatgcgctg agctacgagc gcgttctggc
     3121 gttcgcgcag agcttcattg gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg
     3181 gtgtggttta caccgtatgc accacgcgat gcacgatctg aaaatccacg tgcctgcggg
     3241 caaatgggtt ttctacggtc tggctgctat cctgacagtt gtcacgctga ttggtgtcgt
     3301 tacaatctaa cgcatcgcca atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg
     3361 aaaccagacc ttatgttcaa aacgacgctc tgcgccttat taattaccgc ctcttactcc
     3421 acatttgctg cccctcaaca aatcaacgat attgtgcatc gcacaattac cccgcttata
     3481 gagcaacaaa agatccccgg tatggcggtg gcggtaattt atcagggtaa accttattac
     3541 tttacctggg gctatgcgga catcgccaaa aagcagcccg tcacacagca aacgttgttt
     3601 gagttaggtt cggtcagcaa aacatttacg ggcgtgcttg gtggcgacgc tattgctcga
     3661 ggggaaatca agttaagcga tcccgcaaca aaatactggc ctgaacttac cgctaaacag
     3721 tggaatggga tcacactatt acatcttgcg acctacaccg ctggcggcct gccattgcag
     3781 gtgccggatg aagtgaaatc ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct
     3841 gcatgggctc caggaacaca acgtctgtat gccaactcca gtatcggttt gttcggcgca
     3901 ctggctgtga agccgtctgg tttgagtttt gagcaggcga tgcaaactcg tgtcttccag
     3961 ccactcaaac tcaaccatac gtggattaat gtacctcccc cagaagaaaa gaattacgcc
     4021 tggggatatc gcgaaggtaa ggcagtgcat gtttcgccag gggcgttaga tgctgaagct
     4081 tatggtgtga agtcgaccat tgaagatatg gcccgctggg tgcaaagcaa tttacccccc
     4141 cttgatatca atgagaaaac gcttcaacaa gggatacaac tggcacaatc tcgctactgg
     4201 caaaccggcg atatgtatca gggcctgggc tgggaaatgc tggactggcc ggtcaatcct
     4261 gacagcatca ttaacggcag tgacaataaa attgcactgg cagcacgccc ggtaaaagcg
     4321 attaagcccc caactcctgc agtacgcgca tcatgggtac ataaaacggg ggcgaccggc
     4381 ggatttggta gctatgtcgc gtttattcca gaaaaagagc tgggtatcgt gatgctggct
     4441 aacaaaaact atcccaatcc agcgagagtc gccgccgcct gtcagattct caacgctcta
     4501 cagtaaaatt tcatcgggtc cgaattttcg gaccttttct gcgcttttcc ttgctgtcat
     4561 ctacacttag aaaaaaacca gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc
     4621 agcgacagct gcatttctgg tcgttgcctg tagttttcct acgccgccgc gtggcgtgac
     4681 cgtagtgaat aatttcgacg ccaaacgtta tctcggtacc tggtatgaga ttgcccgttt
     4741 tgatcaccgt tttgaacgtg gactggaaaa agtcaccgca acatacagcc tgcgtgatga
     4801 cggcggcctg aatgtcatta ataaaggcta taaccctgac agaggaatgt ggcagcagag
     4861 tgaagggaaa gcgtacttta ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt
     4921 tggtcctttc tatggcggtt ataacgttat tgcactcgat cgggaatacc gccatgcgct
     4981 ggtttgcggc ccggaccgcg actacctgtg gatactctcc cgcacgccaa ccatttctga
     5041 cgaagtgaaa caggagatgc tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt
     5101 tatttgggta cagcagcccg gtagttagtg agtgctgagt ttcagaccaa taatccccaa
     5161 tacgattaac gccagactcg ccagacgcat cgggttagcg gactcaccga gcagcacaat
     5221 gccggtgatg gccgcgccga ctgcgccaat acccgtccac acggcataag ccgtccctac
     5281 cggtaacgat ttcatcgccc aggcaagtag cgccatactg actatcatcg ccgtcacggt
     5341 aataacactc ggcgtcaaac ggctaaagcc gtgggtatat ttcaggccaa cggcccatac
     5401 cacttccagc agaccagcaa taactaagat aatccaggac atatcaggct ccggaaattt
     5461 ggggccgtcc ccggtgaaaa gaggcgtttg caggtcgtcc tgcaaagctg atggttgaac
     5521 ggatattttg ccaggtggca ggggaatttc aactattacc tttatgacga tcgcagaaaa
     5581 aacaaacggc acgacactgc tgtcgtaccg ttattgctta ttgctgcgct ttcgttgctg
     5641 cgccagaaat cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc
     5701 aggcagttaa tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca
     5761 ccataacgtc ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag
     5821 acaacatcgc caaaaatgat ggaaatctga tgatttttcg gaagagaaga aaaattagct
     5881 ggcagcgcga gagatggagt taccgagatg tttgatgtct tcgccgaaac cgcaagcggt
     5941 gttacagccc gtgagcagcg tgctggcaag caaaacaaga acgataaggc gtttcatcat
     6001 ctctgacctg aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac
     6061 gcgagagacg tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa
     6121 cagcggaact ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc
     6181 ggtatcacct ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg
     6241 agtaacggag atcggctgac cattccacag agttacgata cactctgcct gatccagcag
     6301 ccatttagca ttgtcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt
     6361 gttcatgaag tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac
     6421 atcagcgcct tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag
     6481 cagacgacgc agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact
     6541 cgcttcaacc gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt
     6601 gctatagtac gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa
     6661 tggcgcatat tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg
     6721 atgttgtgac cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac
     6781 tgttggccgg acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg
     6841 atcgcatgga gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt acctcgcaag
     6901 atgagtttgt cgtcgcgtcc ggattctcca ccgacccact ggaagaacag cacagcgtag
     6961 tgcctggttt gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg
     7021 cggtaaattg ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca
     7081 agcgtaactg gcagactgca cttgagtatg ttgctgcgca tccggaactg gacgagatga
     7141 ttttctccgg cggcgatccg ctgatggcga aagagcacga gctggactgg ttgctcacac
     7201 aactggaagc catcccgcat atcaaacgtc tgcggattca cagccgtctg ccgattgtga
     7261 tcccggcacg tatcaccgag gcgctggttg aacgctttgc ccgttctacg ctgcaaatct
     7321 tgctggtgaa tcacatcaat catgccaatg aggtagatga aacgttccgt caggcgatgg
     7381 ctaaattgcg ccgtgtcggt gtcaccctgc ttaaccagag cgttctgtta cgtggtgtga
     7441 acgataacgc acaaacgttg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc
     7501 cgt
//
LOCUS       DAJTYM010000150         7494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTYM010000150 DAJTYM010000000
VERSION     DAJTYM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            384..1973
                     /gene="purH"
                     /locus_tag="OO575_003663"
     CDS             384..1973
                     /gene="purH"
                     /locus_tag="OO575_003663"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6666467.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            1985..3274
                     /gene="purD"
                     /locus_tag="OO575_003664"
     CDS             1985..3274
                     /gene="purD"
                     /locus_tag="OO575_003664"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6666468.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(3271..4596)
                     /gene="zraR"
                     /locus_tag="OO575_003665"
     CDS             complement(3271..4596)
                     /gene="zraR"
                     /locus_tag="OO575_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6666469.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(4593..5969)
                     /gene="zraS"
                     /locus_tag="OO575_003666"
     CDS             complement(4593..5969)
                     /gene="zraS"
                     /locus_tag="OO575_003666"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6666470.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            6207..6632
                     /gene="zraP"
                     /locus_tag="OO575_003667"
     CDS             6207..6632
                     /gene="zraP"
                     /locus_tag="OO575_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6666471.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(6634..7329)
                     /locus_tag="OO575_003668"
     CDS             complement(6634..7329)
                     /locus_tag="OO575_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6666472.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(7342..>7494)
                     /locus_tag="OO575_003669"
     CDS             complement(7342..>7494)
                     /locus_tag="OO575_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR6666473.1"
                     /translation="AVQLVGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKA
                     LKDAVK"
BASE COUNT         1724 a         2096 c         2014 g         1660 t
ORIGIN      
        1 tcttcaaccg aaccggctgt ttgtgtgaag tgattcacat ccgccgtgtc gatgaaggcg
       61 cattataggg agttctccgc aggccgcaat agaaaaattg cagaaaaatg actgactgct
      121 gcattcccca gcaaaacccc gctttatacc tttttacgca cagagttatc cacaatcatc
      181 aatgtaattt ctgtattttt cccacggtaa ccactgtcaa aattgtgatc accattgaaa
      241 gagaaaaatt cgcgagcgtt gcgcaaacgt tttcgttaca atgcgggcga aaaataagga
      301 tgccccgtta ggggcgttag ctgagttttt cgcgaaaaat tcagctaacg ctctctgtaa
      361 tagtcaaacc caggggattt accatgcaac aacgtcgtcc agtccgccgc gctctgctca
      421 gtgtttctga caaagccggt atcgtcgaat tcgcccaggc actttccgca cgcggtgtgg
      481 agctgctgtc tacagggggc actgcccgtc tgttagcaga aaaaggtctg ccggtaaccg
      541 aagtttccga ttacaccggt ttcccggaga tgatggatgg acgcgtgaag accctgcatc
      601 cgaaagtaca tggtggcatt ctgggccgtc gcggccagga cgatgccatt atggaagaac
      661 atcagatcca gcctatcgat atggtggttg ttaacctgta tccgttcgcc cagactgttg
      721 ctcgtgaagg ctgctcgctg gaagatgcgg ttgagaacat cgatatcggc ggcccgacca
      781 tggtgcgctc cgccgctaag aaccataaag atgtcgcaat cgtggtgaag agcagcgact
      841 acgacgccat tattaaagag atggatgaca acgaaggatc gctgacgctt gcaacccgtt
      901 tcgacctcgc catcaaagcc ttcgaacaca ctgccgccta cgacagcatg attgccaact
      961 acttcggcag catggttccg gcgtaccacg gtgaaagcaa agaagccgcc ggtcgcttcc
     1021 cacgcacgct gaacctgaac ttcattaaga agcaggatat gcgttacggc gagaacagcc
     1081 accagcaggc tgccttctat atagaagaga atgtgaaaga agcctccgtt gctaccgcaa
     1141 cccaggttca gggtaaagcc ctctcttata acaacatcgc cgataccgat gcggcgctgg
     1201 agtgcgtgaa agagttcgcc gagccggcat gtgtgattgt gaagcacgcc aacccttgcg
     1261 gtgtagctat cggcaattcc attcttgatg cttacgatcg cgcgtacaaa accgacccaa
     1321 cctccgcatt cggcggcatc attgccttta accgcgagct ggatgcggaa accgcacagg
     1381 ccatcatttc tcgtcagttt gtcgaagtga ttattgcgcc ttccgccagc gaagaagccc
     1441 tgaaaatcac cgccgccaag cagaacgtac gcgttctgac ctgcggtcag tggggcgagc
     1501 gtgttccggg tcttgatttc aaacgcgtga acggcggtct gctggttcag gatcgtgacc
     1561 tggggatggt cggtgcggaa gaactgcgcg tcgtcaccca acgtcagccg accgaacagg
     1621 aactgcgtga tgcgctgttc tgctggaaag tggcgaaatt cgtgaaatcc aatgctatcg
     1681 tctatgccaa aaacaatatg accatcggta ttggcgcggg ccagatgagc cgcgtgtact
     1741 ccgcgaaaat cgccggtatt aaagctgccg atgaaggcct ggaagtgaaa ggttcctcga
     1801 tggcttctga cgcgttcttc ccgttccgcg acggtattga tgccgccgcc gctgcgggcg
     1861 tgacctgcgt aatccagcct ggcggttcca tccgtgatga cgaagtgatt gccgccgccg
     1921 acgagcacgg tattgcgatg ctcttcaccg acatgcgcca cttccgccat taatggagca
     1981 atagatgaaa gtattagtga ttggtaacgg cgggcgcgag cacgcgctgg cctggaaagc
     2041 ggcccagtcg ccgctggttg agactgtttt tgctgctccg ggtaatgcag gcactgcgct
     2101 ggaacccgcg ctgcaaaacg ttgctatcgg cgtgaccgat atcccggcgc tgttggattt
     2161 cgcacaaaac gaaaagattg atctgaccat cgtcggcccg gaagcgccgc tggtgaaagg
     2221 cgtggtcgat accttccgcg ccgccgggct gaaaatcttc ggcccaaccg caggtgcggc
     2281 ccaactggaa ggctcaaaag cgtttaccaa agatttcctg gcccgccata agatccctac
     2341 ggcggaatac cagaacttca ccgaggtaga acctgcgctg gtgtatctgc gtgagaaagg
     2401 cgcgccaatc gtcattaaag cggacggtct ggctgccggg aaaggcgtta tcgtggcgat
     2461 gacgctggaa gaagcggaag cggctgttca cgatatgctg gcgggcaacg cttttggcga
     2521 cgcgggtcat cgcatcgtta tcgaagagtt cctcgatggc gaagaagcga gctttatcgt
     2581 gatggtggac ggcgagcatg tgctgccgat ggctaccagc caggatcaca aacgcgtagg
     2641 cgataaagat aacggaccaa acaccggcgg gatgggcgct tactcccccg cgccggtagt
     2701 aaccgatgaa gttcatcagc gcaccatgga acgtatcatc tggccaaccg tgaaaggcat
     2761 ggcggcggaa ggcaacacct acaccggttt tctctatgcg ggcctgatga tcgacaaaca
     2821 gggcaatccg aaggttattg aatttaactg ccgctttggc gatccggaaa cccagccgat
     2881 tatgctgcgc atgaagtccg atctggttga gctctgcctg gcggcctgtg aaggcaaact
     2941 ggacgagaaa acgtcagagt gggatgaacg tgcttctctc agcgtggtga tggctgcggg
     3001 tggatatccg ggcgattacc gcaccggcga tgtgatccac ggcctgccgc tggaagaagt
     3061 ggctgacggc aaagtgttcc acgcgggcac aaaactggcg gatgacgagc aggtggtaac
     3121 cagcggcggg cgcgtactgt gcgtcaccgc gctgggtcat accgtggcag aagcgcagaa
     3181 acgcgcctac gccttaatga ccgatatcca ctgggatgat tgcttctgcc ggaaagatat
     3241 cggttggcgc gccatcgaac gcgagcagaa ctaacgcgac agttttgcca atagcgtttt
     3301 gcgcgtgatc cctaactgac gggcggcttc ggttttgttg ccgcccgttt tctccagcgc
     3361 cgccagaatc acctcttttt ccacttccac cagcggctga atatcctgac tttgccccag
     3421 cgggatcggc gtactggcaa tcgccagcgg cagctcgcgt tcggaaatat attccccggt
     3481 cagcagcacc accgcccgtt ccaccgcgtt ttccagttca cgaatatttc ccggccagtc
     3541 gtagtggatc agcaaatcca tcgcctgtgg cgtaaagcct tttaccgcct tgcgattacg
     3601 ctcggcaaag cgctgaagaa aatggccagc cagcagaggg atatcttccc gccgttgccg
     3661 cagcgatggt acttcaatcg ccaccacatt caggcgatag tagagatcct ggcgaaaacg
     3721 cccggcattc acctctgcgg caagatcgcg atgggtcgcc gcaatcagcc ggacatcaac
     3781 cgagatagtc tggttgctac cgacacgctg aacttcgcgc tcctgaatcg cacgcagcag
     3841 acgcacctgc atcatcggcg agatatcgcc aatttcatcg agaaacagcg tgccgccgtc
     3901 cgcctcaaca aagcgccctt cccggcgttt atcggctcca gtaaacgccc ctttttcgtg
     3961 accgaacaat tcagattcca gcaaggattc gttgagtgcc gcacagttga gcgttaccag
     4021 cggtttttcg ctacgtgcgc tactggcgtg aatcgccctg gcgaccagct ctttaccggt
     4081 gccggaatcg ccgtggatca gcaccgtggc ttccgatggc gcgacgaggg cgatttcact
     4141 gagcaggtgt tgcatcgccg ggcttttacc gaccataccg aactggctgg cagtcaccgc
     4201 aggtgtttca gcatcaatac tgtgcgtatg cgcgagcgcc ttttccagcg tcgcctgtag
     4261 gttatcgaaa tccagcggct tgatgagata atcctgcgcc ccagttttca gtgcctctac
     4321 cgccgtctcg acgctggagt acgcagtcat aatcagcacc ggaattgccg ggtttaacgc
     4381 tttgatctct ttcagcgtgg cgatgccgtc catctccgcc attcgcacat cgcaaagcac
     4441 aagatcaaaa acctgttccc gcacctgctc cagcgcctgt cgcccgctgt tcgccagcgc
     4501 gacgttatag ccccagccgc gcagtaaagc ctgcaaaata gtgcagtggc taatgtcatc
     4561 atccaccacc agaatatcga tattatcgtg cgtcatcctt gtgggtcctt acgcgtaata
     4621 ttgaccggaa gccagagggt gaacgttgag ccttttccct cctggcttgc gacctgaatt
     4681 gtaccaccgt gttgttcaac aatattatgc acgaccgcca gccccaatcc ggtgccttcg
     4741 gctttggtgg tgaagtacgg agtgaagatg gcttcaagct gatctgccgc aattccctta
     4801 ccgctgtcgg taacgctgat tttcacgccc gcgccgcttt cgctggccgt cacgctaatc
     4861 acgccatgct gaccaatcgc ctgaatagca ttgagataga gattcaacag gacctgagtc
     4921 agcctgtccg gatcggcctg aatttccggt aatgtgtcgt tggcggtaaa gcgtaactgg
     4981 atctcccggc tgtttgcatc ctggcttacc agctgtaatg agtggttaat cagcgtgttg
     5041 agatccaccg cctgcaaagc cagatgcgtt ggcttaacca gttccagcaa ctcgcttacc
     5101 acgcggttta aacggtcggc ctctttcgcc atcacctgcg ccagttgatg cgcttctccc
     5161 cctgcaggcg cgcgctcggc aaagtatttc gccagtcctt taatcgagga aagtgggtta
     5221 cggatttcgt gggcaacgcc tgccgcaaga tgccccagcg ccaccagctt ctctttgcgc
     5281 ttcatttcat cttgtagaag ctggcgcgag cgcagatagc ggcgatacca gaagaatgac
     5341 aatacgcttg ccagcaagac cgtcgccagg gcgaagagaa taatcagggt gtttctctgc
     5401 tcacgatctt cggcactcac aatattactg gcgtcgaaag cgatgaaaat tgcttgtggt
     5461 gtcgcggcat actgctgcat atggcgcatc cggtacattc cagcagcaaa catcggttga
     5521 aactggcgat aaatttccag aacaggctcg ccgtctgcgc tatcgatccg ccgccacgct
     5581 tcttcatctc ccggatgtaa ctgctgcatt tcctgagggg aataaagctg ttttcccacc
     5641 atgccggagt tgctatgcat cacgattgtt ccttgttcat ccgtgaccgc aaaccaacgt
     5701 actccaggct gcccggccat ttcttccagt aaggcctgct gctgcgcatg atgcatgcgc
     5761 atccccatgc cgacgcgcga gccggattca agagcgcgaa taagtacact gcctttttcc
     5821 agcagcgttt gtctggcggc ggcagtctcg cgcccataat cgcgaatcac cgtcaccgca
     5881 aacaacccta ccagcccaac aatgaccacg gggaggatcg cgcttaacca tttagctaag
     5941 gagtctttag aacgttgcat aaaacgcatc ttcttctttg cctgctcatc cctttctgac
     6001 agcagaaatc atgccatctt ttatcagcgc ttaccctgcg ctgtaacaca aaggcttaag
     6061 tttcaatgag taaaaatgac tcgctacccg cagcaggcga gtcattttta ctcgtttatc
     6121 atgccagatt acccgtcata tcagcgtttc atcgttggca cggaagatgc aatacccgaa
     6181 gtaagacaac cactggagga ttaaccatga aacggaacac gaaaattgcc ctggtaatga
     6241 tggcgctttc agcaatggcg atgggatcga catctgcatt tgctcacggc ggacacggta
     6301 tgtggcagca aaatgccgcg cctttgacca gcgaacaaca gacagcgtgg cagaaaatcc
     6361 ataatgactt ttacgctcaa agcagcgcac tgcaacagca actggtgacg aagcgttatg
     6421 aatacaatgc cctgttagcc gcgaacccac cggatagcag caaaattaac gcggtcgcca
     6481 aagagatgga gaatttgcgt cagtcgttag atgagttacg ggtgaaacga gatatcgcga
     6541 tggctgaagc gggtattccg cgcggagccg gaatgggcat gggctacggc ggctgcggtg
     6601 gtggcggtca tatgggtatg ggccactggt aaatcagaac gttttctcgt tgggttgcca
     6661 gcgacagaaa tcagagtttg ccactaacag taattgcgaa ccttcgggcg cttccagcca
     6721 tgccacgctg acatcaacag aagagcggct ctggcggcgc tcgatatgcg ctatcgcctg
     6781 cgaatctaaa tcaggtttaa tggtttgccc ttcgcaggtg gtcacggtct gttccatcgc
     6841 atgccagcgc ccctggcgca gcacgactct cccctgacgt aaggcatcgc taatctggcg
     6901 gatctgatca gcacgataac ggtataaatc gatttggtcg ttagaaagct gctgcttttc
     6961 cccatcggat tcacgttgca taaagctcag atcgccgcga tcgtcaaaac gcgcgcgaat
     7021 gttcaccggc ggtttgctgt aaacattgat attgatgagc gtgaggttat cgccctgcca
     7081 gcgatactct cgcgtcgtgg tatcgccact gcgccacggg ctaaacacgg caagcagatg
     7141 cacattatcg ccgctgtctt ttcgccagat gcgtaccgcg ccctggtctt cagcaaatcc
     7201 actggcggta aagggaggaa gtgaagagtt gtggctacag gcagtgagaa acagcgcacc
     7261 tgccagcatc aatgatcgac gccagaaaga caaaaggggt gaaaccaccc cttcgttaaa
     7321 actgttcact gccacgcaat cttacttaac tgcgtctttc agtgccttgc cagaaacaaa
     7381 tgccggtacg ttagctgcgg caattttgat ttctttaccg gtctgcgggt tgcggccagt
     7441 acgctcagcg cgatggttca ctttgaaggt accgaaacca accagttgta cagc
//
LOCUS       DAJTYM010000151         7449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTYM010000151 DAJTYM010000000
VERSION     DAJTYM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(28..2940)
                     /locus_tag="OO575_003670"
     CDS             complement(28..2940)
                     /locus_tag="OO575_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666474.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            2943..3059
                     /locus_tag="OO575_003671"
     CDS             2943..3059
                     /locus_tag="OO575_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6666475.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3423..4265
                     /locus_tag="OO575_003672"
     CDS             3423..4265
                     /locus_tag="OO575_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6666476.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4268..5356
                     /gene="capU"
                     /locus_tag="OO575_003673"
     CDS             4268..5356
                     /gene="capU"
                     /locus_tag="OO575_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6666477.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5361..6311
                     /gene="virK"
                     /locus_tag="OO575_003674"
     CDS             5361..6311
                     /gene="virK"
                     /locus_tag="OO575_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6666478.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6376..7320
                     /gene="lpxM"
                     /locus_tag="OO575_003675"
                     /gene_synonym="msbB"
     CDS             6376..7320
                     /gene="lpxM"
                     /locus_tag="OO575_003675"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6666479.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2018 a         1643 c         1740 g         2048 t
ORIGIN      
        1 tcggttacat cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc
       61 cacccagtat ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat
      121 atccactaat tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc
      181 cggtgactgc aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg
      241 gttaagaata tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt
      301 gctgttaact tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc
      361 cggcagcaac gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc
      421 ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac
      481 cagaaacatt gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga
      541 cagactttca ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg
      601 cgtactgacc gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag
      661 caagggatct gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc
      721 tgctgacatc agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat
      781 atcccgcaac acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag
      841 taactgcaga gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc
      901 attatacagc gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt
      961 tttatcggct gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat
     1021 aatctgccat ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc
     1081 ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat
     1141 ctcttcctgc agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg
     1201 atgctccacc tgctgttcaa caagatacac attgtgttta gcacgggtga gcgcaacata
     1261 cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg
     1321 ggaataacgg gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa
     1381 tgcctgccgg gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt
     1441 aacctcctga accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg
     1501 ttccggatgc atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc
     1561 ctgctttgtc tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct
     1621 tctgacaaaa tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag
     1681 caaccggtta gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt
     1741 aacggttgtg tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga
     1801 gaaaaagctc ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg
     1861 acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac
     1921 aaaaatgaca tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact
     1981 gtcatactga tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt
     2041 cgggcggtcc tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg
     2101 ggacagcggc ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag
     2161 ggtatgggca gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc
     2221 actgaaagtc acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga
     2281 aagaaagtca atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc
     2341 tttttccacc agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat
     2401 tttttccagt accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag
     2461 cgggtgttcg tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg
     2521 gaaacgccca tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc
     2581 cggcactggt tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg
     2641 tgaagtgtta taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg
     2701 gtagcgatac agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt
     2761 ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa
     2821 ttttttataa cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc
     2881 tgccggtttc cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa
     2941 ccttgcccgg aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat
     3001 ggaataaaga aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc
     3061 tgcaggcctg tcatgatgca ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc
     3121 cgtaaataaa ctctgcgctg aaattccttt ttttcattgg agtacctgtg ttgttctgag
     3181 gtgaacatat cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt
     3241 gagtcgtttt taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt
     3301 agtaaattat atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata
     3361 aaatgcacca gctttattgg gttttccttt tgtctctgct tgctaggtgc cagaaacttt
     3421 aaatgttaaa cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc
     3481 atcatgtcag tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa
     3541 taaaatggtt ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt
     3601 atcggggcgg aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg
     3661 acaactggtt tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt
     3721 taattaccgg ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc
     3781 atcgcgattg cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt
     3841 ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc
     3901 atacacgctg ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg
     3961 acttgttgag cgggagggaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt
     4021 gttggccgga aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct
     4081 attatttata tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag
     4141 gaagaataag caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt
     4201 atacaacccc gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg
     4261 attaataatg aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca
     4321 ggctgttgct caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag
     4381 ggaaaacagc aaaattgctt ttgaagccag taaattgggg attgatatca cattcgcgtt
     4441 atttcgaaac agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt
     4501 tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg
     4561 gttattcact aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa
     4621 aacaaaagtt ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat
     4681 gaagacacat ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt
     4741 tgatttccag aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg
     4801 gctggcgtcc cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa
     4861 agggcacgaa tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg
     4921 ttggttgatt gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga
     4981 cagtatgggc atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt
     5041 atatcgggtt gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact
     5101 ggcagaagca tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga
     5161 tgttattcag aataaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat
     5221 gtgcgccctg aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc
     5281 aaaacaggat atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt
     5341 agcgaagcac aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta
     5401 aaaggattgt tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt
     5461 tcatgtacat tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac
     5521 tgcgttcctt gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa
     5581 ttcatcgacc ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg
     5641 agcattatcg ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa
     5701 aatcggtatc attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt
     5761 ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aataaaatag
     5821 tgattgcccg gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg
     5881 gcggattaca gggagccccc aaaaataccg gacctgatat tattcggtgc gcaacccggg
     5941 cctgttatgg gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag
     6001 cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga
     6061 gatactggta tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg
     6121 tagcagggaa aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc
     6181 taagcaatat agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt
     6241 atatccatga aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg
     6301 acttaaatta aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg
     6361 cattcgcact aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat
     6421 tatctttccc ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca
     6481 atgtttcccc cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga
     6541 ttgagcagaa aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag
     6601 agtgatacag aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata
     6661 gtgatgatgg cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg
     6721 aaaggcctcg aaattcttga ggaaattcgg cacaataaca gaaatgtgat ttttctggtt
     6781 ccccatggct ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg
     6841 gccgccatgt ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg
     6901 cgtaaattcg gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca
     6961 gtacgccagg gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt
     7021 gaatttgctg atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg
     7081 aacatcagtc aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc
     7141 ctgactattg aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg
     7201 attgcccgac aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat
     7261 atctgggttt taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga
     7321 aattataaag tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat
     7381 cccccttccg tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt
     7441 taatttatg
//
LOCUS       DAJTYM010000152         7434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTYM010000152 DAJTYM010000000
VERSION     DAJTYM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..764)
                     /locus_tag="OO575_003676"
     CDS             complement(<1..764)
                     /locus_tag="OO575_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6666480.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKN"
     gene            complement(767..1972)
                     /gene="hemX"
                     /locus_tag="OO575_003677"
     CDS             complement(767..1972)
                     /gene="hemX"
                     /locus_tag="OO575_003677"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6666481.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(1994..2734)
                     /gene="hemD"
                     /locus_tag="OO575_003678"
     CDS             complement(1994..2734)
                     /gene="hemD"
                     /locus_tag="OO575_003678"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6666482.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(2731..3672)
                     /gene="hemC"
                     /locus_tag="OO575_003679"
     CDS             complement(2731..3672)
                     /gene="hemC"
                     /locus_tag="OO575_003679"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6666483.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4059..6605
                     /gene="cyaA"
                     /locus_tag="OO575_003680"
     CDS             4059..6605
                     /gene="cyaA"
                     /locus_tag="OO575_003680"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6666484.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(6645..6965)
                     /gene="cyaY"
                     /locus_tag="OO575_003681"
     CDS             complement(6645..6965)
                     /gene="cyaY"
                     /locus_tag="OO575_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6666485.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7013..>7434)
                     /locus_tag="OO575_003682"
     CDS             complement(7013..>7434)
                     /locus_tag="OO575_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6666486.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1656 a         1970 c         1935 g         1873 t
ORIGIN      
        1 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
       61 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
      121 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
      181 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatccacg
      241 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
      301 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
      361 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
      421 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
      481 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
      541 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
      601 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
      661 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
      721 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
      781 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
      841 caggtttagc ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct
      901 gcatcagttt ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat
      961 tttgctggct taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat
     1021 cgtagtaagc acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct
     1081 gatgacgcgg tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat
     1141 agatatcctg atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa
     1201 tgaagttgtc cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg
     1261 aactggaaag ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca
     1321 gacgcaggtt atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat
     1381 ccacctgcga tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa
     1441 tcagactcgg gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag
     1501 aggtcgtgac gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg
     1561 cctgagccag cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga
     1621 cttcatccaa ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag
     1681 cttgttgctt aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg
     1741 ccgtcagttg gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac
     1801 cccagccata caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa
     1861 tcaatgcagt attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca
     1921 cggcctccct ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt
     1981 cctgttatga gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga
     2041 ccttaatgtc ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca
     2101 atagtcgaca gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt
     2161 gctgcaacat ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca
     2221 tcgcttcttc tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga
     2281 cctcagcacc gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta
     2341 atatcagcgc acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc
     2401 tgatttcccg atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg
     2461 cggtggtgcg tccaatggcg aaataagtag gtagttgggg ccatttacga tcttgctgat
     2521 gcagctgtga ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct
     2581 cccccagcgc tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa
     2641 tcagcggaaa atgccaggcc acctgcccca gtgtgcgcag acggctcact aactcttctc
     2701 cagcgggaga cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc
     2761 agcgaggatc tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg
     2821 ttcggcatct tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc
     2881 gccgaccagc gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg
     2941 cacctgacat ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc
     3001 agtttcgtgg tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca
     3061 ttcaataccg actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc
     3121 gcgaatgcgg gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc
     3181 gtattcgccg ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat
     3241 aatcagatcc gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac
     3301 gatactgcct gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg
     3361 aggatcttca cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac
     3421 atctttcatt gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc
     3481 ttttacaaat aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc
     3541 gcgcgtcacc atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc
     3601 tttgacatag tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac
     3661 attgtctaac atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca
     3721 gagtgatgtc agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg
     3781 gcagtggatc ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg
     3841 gtgctacact tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga
     3901 tcacgtttta gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa
     3961 cggttacctt tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga
     4021 atcacagtca tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc
     4081 tgaaacagag actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg
     4141 ggcctgcatt ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc
     4201 gaatgccggg ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg
     4261 aaactcaacg ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc
     4321 caccgaaagg tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag
     4381 ggcaaagttg ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg
     4441 aagagcgcca gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg
     4501 gtgtggaagt cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca
     4561 gcctgggggg cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc
     4621 gtactgccgt gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag
     4681 aagagcatta cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg
     4741 aatggctgga tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc
     4801 tttggcagct ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc
     4861 tggaagccta ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc
     4921 gtttgcacga cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc
     4981 gtgttactga atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct
     5041 gcttctattt aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc
     5101 gcgcagtgtt gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc
     5161 tcgataaccg tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg
     5221 acgcgatgat gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg
     5281 tctccgccag tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag
     5341 cattgccagg taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga
     5401 atctgacctt tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata
     5461 accgcgcgcc aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc
     5521 tgaataaact ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt
     5581 atattaaagg taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt
     5641 cgcaccattt cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg
     5701 agattcgcca tctggcgatt atcgttaacc tggaatatga cccgacagct gcgttccgca
     5761 atcaggtggt gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa
     5821 attgcctggt aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc
     5881 tgcacttcaa cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc
     5941 atcaggacgc cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg
     6001 gcttaatccg tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca
     6061 gcacccgcca ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct
     6121 tgttcttcga acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg
     6181 gcgctatttc gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca
     6241 aattaccggc ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag
     6301 aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg
     6361 tgtatcacca ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact
     6421 cgtcatcgca cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt
     6481 tctatcagat tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga
     6541 ttggcaacct gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt
     6601 cgtgatgagc gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact
     6661 gtttcacccg cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg
     6721 cgatcgcaaa tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc
     6781 agccacacct ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag
     6841 gtaatggtca gtacgccgcc gttgatttcg cagtcgatat cgctgtcgcc atccctgtcg
     6901 tccagacgct cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg
     6961 ttcattgttg tatcctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct
     7021 tcaaagtttg cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga
     7081 tgtctctgtt ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg
     7141 gggagggcgt aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag
     7201 cgaagcgatc gtttttagtt agcgaaccgt taactggaat aatttgcaag ctctttgacc
     7261 gtaattggcg gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca
     7321 ggtctggcgt atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg
     7381 ttacatacag ccgatcttta tattgcgcaa tattgccagt aatcagatgg aatt
//
LOCUS       DAJTYM010000153         7024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTYM010000153 DAJTYM010000000
VERSION     DAJTYM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..894
                     /locus_tag="OO575_003683"
     CDS             <1..894
                     /locus_tag="OO575_003683"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6666487.1"
                     /translation="ARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTY
                     TGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSIS
                     ASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYY
                     EFLRLGREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGED
                     PGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASL
                     KYLSDHPKLQGIAQQNSFKHT"
     gene            1264..2088
                     /gene="gadX"
                     /locus_tag="OO575_003684"
     CDS             1264..2088
                     /gene="gadX"
                     /locus_tag="OO575_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6666488.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            2456..3184
                     /gene="gadW"
                     /locus_tag="OO575_003685"
     CDS             2456..3184
                     /gene="gadW"
                     /locus_tag="OO575_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6666489.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3547..6660)
                     /gene="mdtF"
                     /locus_tag="OO575_003686"
                     /pseudo
     CDS             complement(3547..6660)
                     /gene="mdtF"
                     /locus_tag="OO575_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(6685..6942)
                     /locus_tag="OO575_003687"
                     /pseudo
     CDS             complement(6685..6942)
                     /locus_tag="OO575_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1805 a         1693 c         1699 g         1827 t
ORIGIN      
        1 gcccgctact gggatgtgga gctgcgtgag atccctatgc gccccggtca gttgtttatg
       61 gacccgaaac gcatgattga agcctgtgac gaaaacacca tcggcgtggt gccgactttc
      121 ggcgtgacct acaccggtaa ctatgagttc ccacaaccgc tgcacgatgc gctggataaa
      181 ttccaggccg acaccggtat cgacatcgac atgcacatcg acgctgccag cggtggcttc
      241 ctggcaccgt tcgtcgcccc ggatatcgtc tgggacttcc gcctgccgcg tgtgaaatcg
      301 atcagtgctt caggccataa attcggtctg gctccgctgg gctgcggctg ggttatctgg
      361 cgtgacgaag aagcgctgcc gcaggaactg gtgttcaacg ttgactacct gggtggtcaa
      421 attggtactt ttgccatcaa cttctcccgc ccggcgggtc aggtaattgc acagtactat
      481 gaattcctgc gcctcggtcg tgaaggctat accaaagtac agaacgcctc ttaccaggtt
      541 gccgcttatc tggcggatga aatcgccaaa caggggccgt atgagttcat ctgtacgggt
      601 cgcccggacg aaggcatccc ggcggtttgc ttcaaactga aagatggtga agatccggga
      661 tacaccctgt acgacctctc tgaacgtctg cgtctgcgcg gctggcaggt tccggccttc
      721 actctcggcg gtgaagccac cgacatcgtg gtgatgcgca ttatgtgtcg tcgcggcttc
      781 gaaatggact ttgctgaact gttgctggaa gactacaaag cctccctgaa atatctcagc
      841 gatcacccga aactgcaggg tattgcccag cagaacagct ttaaacacac ctgataacat
      901 aacgttgtaa aaaccgaatg cccagccttt aaaaaaacag ctgggcattc ggttgcttat
      961 taatcgcaat aatatattgg ctgtctattc atcgtgttga taagatatat gttatatgaa
     1021 tgtttattaa atgtagcgat tacctttaat gtcacgaaaa gggattattt gcttgctatt
     1081 aatttcactg cgttcttaaa cacgtttttg tattataaat ccagtcatcc tgcccgtaga
     1141 acatcacaca ttatcatcct gttctcccgc ttcgtttaaa tttatttatc aatcaatttg
     1201 acttaagagg gcggcgtgct acattaatta acagtaatat gtttatgtaa tattaagtca
     1261 actatgcaat cattacatgg gaattgtcta attgcgtacg caagacataa atatattctc
     1321 accatggtta atggtgaata tcgctatttt aatggcggtg acctggtttt tgcggatgca
     1381 agccaaattc gagtagataa gtgtgttgaa aattttgtat tagtatcaag ggatacgctt
     1441 tcattatttc tgccgatgct caaggaggag gcattaaatc ttcatgcaca taaaaaaatt
     1501 tcttcattac tcgttcatca ctgtagcaga gatattcctg tttttcagga agttgcgcaa
     1561 ctatcgcaga ataagaatct tcgctatgca gaaatgctac gtaaaagagc attaatcttt
     1621 gcgttgttat ctgtttttct tgaggatgag cactttatac cgctgctttt gaacgttttg
     1681 caaccgaaca tgcgaacacg agtttgtacg gttatcaata ataatatcgc ccatgagtgg
     1741 acactagccc gaatcgccag cgagctgttg atgagtccaa gcctgttaaa gaaaaaattg
     1801 cgcgaagaag agacatcata ttcacagttg cttactgagt gtagaatgca acgtgctttg
     1861 caacttattg ttatacatgg cttttcaatt aagcgagtcg cagtgtcctg tggatatcac
     1921 agcgtgtcgt atttcattta cgtctttcga aattattatg ggatgacgcc cacagagtat
     1981 caggagcgat cggcgcaggg attgccgaac cgtgactcgg cggcaagtat tgttgcgcaa
     2041 gggaattttt acggcactaa ccgttctgcg gaaggaataa gattatagag ttttactcag
     2101 acataaaaaa aacccggcat aggggaccgg gaagaggata gtctgccgtc tccagactaa
     2161 taaaccgtta taacactccc tgttggcacg ggaaactttg tgctatcagt aagttaaata
     2221 taacttttac aggaaataag atcagccatt tttttataaa cataagctat acgctgtgcg
     2281 aaaatatatt cgtgctgcat ttacttatta tcaattaact gttatgcaaa actactttgt
     2341 ggataaattt tggtcctacc aaatctggca gtttttgcgc taagaaacag tctgtcatca
     2401 tttcattagt atactgaaat tgaaataatc gcagtatgaa atataaggga taatcatgac
     2461 tcatgtctgc tcggtgatcc tcattcgtcg ttcattcgat atttatcatg aacagcaaaa
     2521 aatatcgctg cataacgaga gtatcctgct gctggagaaa aatttggcag acgattttgc
     2581 gttttgttca ccggatacgc gacgactgga tatcgatgag ctgacagttt gccattactt
     2641 acaaaatatt cgtcagctac cacgcaattt agggttacat agcaaagacc gtctgttaat
     2701 taaccagtca ccccccatgc cgctggtgac ggcgattttt gatagcttca atgaatccgg
     2761 ggtaaattca ccgatactga gcaatatgct ctacctttcc tgtttatcga tgttttctca
     2821 taagaaagaa ctgatcccct tacttttcaa tagcatcagc actgtttcag gaaaagttga
     2881 acgccttatt agctttgata tcgccaaacg ttggtatctg cgcgatatcg cggaaagaat
     2941 gtataccagc gagagtctca tcaaaaaaaa gttgcaggat gaaaatacct gtttcagtaa
     3001 aatattactc gcttccagga tgtcgatggc cagacgatta ctcgagttac gtcaaattcc
     3061 tctgcatact attgcggaaa aatgtggcta tagcagtacg tcgtacttta taaacacatt
     3121 tcgacaatat tatggtgtaa cgccacatca gtttgcgcaa cattcgccag gtaccttttc
     3181 ctgacatatt ttgcatttga atattggtca ggatctcaca cctgcttcat gtgaaactct
     3241 tccctgatga tttctgccgg gctaccggct agttctcttt cgcagtttgc caggcggtat
     3301 aggtattttt aggaggaaaa gactcggcat gtttgggatt attaagctga caattcatac
     3361 gattacggga atgtgggtca gtattgtgtt gttcaaattg atgacgaatg gctggagtgg
     3421 tttctacttt cagtgctgtg tactaagcct ggtattcctg acggttagct ggttgttgag
     3481 tggtgaatgg ttagcaggaa agagtaaggc tgaaccttca cgttcaacct tactctcatt
     3541 tacacgttac gcttttttaa agcgggcaaa gagatgttcc actacaacga aaaagaccgg
     3601 aacgaagtaa attgccagca ctgttgcggc aaacatcccg cccatcacgc cggtacctac
     3661 cgcgttttgc gcgccagaac cggcaccatg actgataacc agcggcagca cgccgagaat
     3721 aaaggccaga gaggtcatca ggattgggcg taaacgcatc cgcgccgctt cgatgattgc
     3781 ctctatcggc gttttccctt ctttctgcat catctcaacg gcaaattcga caatcaggat
     3841 ggcgttcttg gcggaaagcc cgatggtggt cagcaaacca acctggaagt agacgtcatt
     3901 acttaagccg cgcagatcgg tggccagtaa tgcgccaacg acgcctaacg gaacaaccaa
     3961 catcaccgag aacggaattg accagctctc atagagtgcg gcgagggcga ggaacaccac
     4021 gaccagtgaa atggcataca gtgcaggagc ctggtttgag gataacgctt cctgatacga
     4081 cagtccggtc catgagtagc cgacgcctgc cggaagttta gcgaccaggt ctgccataaa
     4141 tttcatagcg tcaccggtac ttttcccggc cgccgcttca cctaaaatct ccattgacgg
     4201 gatgccgttg tagcgttcca atcgcggtga accataggtc cattcggtag acgagtaggc
     4261 agaaagcggt gccatcgtgc cagaggcgtt gcgtacatac cattggttga tgttatccgg
     4321 caacatacgg aacggcgtgc ctgcctggac atacactttt ttcacccgcc cctggttgag
     4381 gaagtcgttc acgtagctgc tgccgaaggc ggtggaaatt gtctggttga tatcagacag
     4441 cgccacaccc attgcctcag ctttcgcggc gttgacgttc actttgaaca tcggcgtatc
     4501 ttccaggccg ttcgggcgta ccccggtgac ctggtcaggc gattgcgctg ccagtgataa
     4561 cagctcgttt cgtgcctagg ttagtttttc gtgccccagg ttaccgttgt ccagcagttc
     4621 catatcaaaa cctgacgcgg tacccagttc agccaccgcg ggtaagttga acgggaagac
     4681 gacggcttta ttgatactgc ttaacgcaat cattgcccgc tgaatgatcg cggtaaccga
     4741 gttttcctca ccgacacgtt cagaccacgg cttgagactg ataaacgcca ggccgttgtt
     4801 ttgcccctgg ccgctgaagc caaagccgcc aacggtaaac accgactgaa cattatcttt
     4861 ctcttttgta agataataat ccgtcacctg ttgcagtact ttcgtggtgt taaccatggt
     4921 ggcaccggaa ggtaactgcg cggtggtcat aaataccccc tgatcctctt ctggtaagaa
     4981 agaggtcggt gtacgcagga acagcaccgc catcccggcg caaatcagca ggtagaccac
     5041 catgtagcga ccggtacaac gcaacagcga gcgggtgcta tcggtatagt gctgagttga
     5101 tttttcaaac agcgtgttga agcgtgcgaa cagggcgtta ggtttgtgac cgccttccgg
     5161 cgtggctttc agaatggtgg cgcacagggc aggggtcagg ctcattgcca caaatactga
     5221 aagccgcatg gaggagatca gcgtgatgga gaactggcgg tagatctccc cggttgcacc
     5281 gctcataaag gccatcggca taaacactgc agaaagaaca acggcaatcc cgaccagcgc
     5341 acgttggatc tgccccatcg atttatgcgt cgcttccttc ggcggtagct tatcttccgc
     5401 aatgacacgc tcgacgttct ccaccaccac gatggcgtca tccaccagta accctatcgc
     5461 cagcaccatc ccgaacatag tcaacgtgtt gatggtgaaa ccgaccgccg acaagatcgc
     5521 aaacgtcccg agaataacca ccggtacggc aattgtcggg atgatggtgg cacggaaatt
     5581 ctgcaaaaac agataaatga ccaggaagac gaggatgata gcctcaacca gtgttttgaa
     5641 aacttcctga atagaaattt cgataaacgg cgtggtgtcg taaggataaa ccgtcttcag
     5701 acttgcgggg aaataggctg ataagcggtt cagctcctct ttgactgccc gcgaggtatc
     5761 cagggcgttt gctccggcag ccagtttgat ggcgatcccg gcaacaggtt tgccgttata
     5821 gcgcgccacg gtggaataat cttccgcccc aagttcgacg cgggcgacat cacgcagcag
     5881 cacttgcgaa ccatcttgct gaactttcaa caggattttg ccaaattctt ccggcgtttg
     5941 cagacgcgtc tgcacaatga tcgaggcgtt tagttgctgg tctgccgcct gtggcatgcc
     6001 acccagttga ccaccagaaa tctggttgtt ttgcacctta atctgggaaa taacatcgga
     6061 aggtaccagg ttatatttat tgagtttttg cgggtccagc cagatacgca tggcgtactc
     6121 ggaaccaaag agctgtacgc taccaacgcc cgcggtacgg cttagcgggt ctttgatatt
     6181 agacgctaca tagtccgcga tatcgtactg gttgaggctg ccgttatcag aaataaacgc
     6241 cgctaccatc aggatattac tgctcgactt atcgacgcta atcccctgtt gctgcaccgc
     6301 ttcaggtaat gaaggcatag cgagttgcag tttattttgc acctgaacct gtgcgatatc
     6361 aggagatgcc ccagtctcga aggtcagagt gatagaggca ttacccgccg catcactggt
     6421 tgaagacatg tacatcaggc catcaagccc attcatattt tgctcaatca cctgagtgac
     6481 cgagtcttct accgtttgcg catcggcacc cggataggta gcgctgacgg taatggtcgg
     6541 tggcgcaatc tgcggatact gcgcaaccgg taagttcatg atcgccagac cacctgcaag
     6601 catcataata atggcaagta cccaggcaaa aaccgggcga tcaataaaat agttagccat
     6661 gaaagtcccc ttaagcctgc aacgttattg tttcgattcg gtgctggcgt tttcctggct
     6721 ggaggaaatt gctcgtgctt tgatacccgg acgaatgcgt tgcaaaccgg aaacgatcac
     6781 ccgatcgcca gcctgcaagc cagaggtgac aacccactgg tcgccgatgg ctttgctggc
     6841 ttcaatttcg cgtagctgca cgacatcgtc tttatccaga atgagcgccg ttgctttacc
     6901 ctgggcgttg tgggtgacgc cttcctgcgg caccagtaat ataaagcgcg caaaattatt
     6961 caagatattt ttgaaaatca ttcaagatag gctatcgcgt gtgatagcaa aaacattcaa
     7021 gata
//
LOCUS       DAJTYM010000154         6979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTYM010000154 DAJTYM010000000
VERSION     DAJTYM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..529
                     /locus_tag="OO575_003688"
     CDS             <1..529
                     /locus_tag="OO575_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6666490.1"
                     /translation="RLYRCVCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQC
                     GLVTVFILLVSFGGGTASAFFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVI
                     FKTRMSAMIATVVVVLVVTFANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQ
                     NYCSRMANKPELALD"
     gene            complement(680..3991)
                     /gene="mscM"
                     /locus_tag="OO575_003689"
     CDS             complement(680..3991)
                     /gene="mscM"
                     /locus_tag="OO575_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6666491.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4013..4981)
                     /gene="psd"
                     /locus_tag="OO575_003690"
     CDS             complement(4013..4981)
                     /gene="psd"
                     /locus_tag="OO575_003690"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6666492.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5078..6130)
                     /gene="rsgA"
                     /locus_tag="OO575_003691"
     CDS             complement(5078..6130)
                     /gene="rsgA"
                     /locus_tag="OO575_003691"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6666493.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6225..6770
                     /gene="orn"
                     /locus_tag="OO575_003692"
     CDS             6225..6770
                     /gene="orn"
                     /locus_tag="OO575_003692"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6666494.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1584 a         1867 c         1890 g         1638 t
ORIGIN      
        1 tcgcttatac cggtgcgttt gctattcacc gctgaaagcc atcatccagg ggacgccgaa
       61 agcgttgtgg ccggaaccga tgacgcgcct gaatgcgatg gggatgccgt ctatcgccat
      121 gtggatgcag tgcgggttgg ttactgtctt cattctgctg gtttcgtttg gtggcggtac
      181 cgcatcggcg ttctttaaca agctgacgct gatggcgaac gtgtctatga cgcttcctta
      241 cctgttcctc gcgctggctt tcccgttttt taaagcacgt caggatctcg acagaccatt
      301 tgtgattttc aaaacgcgta tgtcggcaat gattgcgacg gtggttgtcg ttctggtggt
      361 gacatttgcg aacgtcttca ccattattca gcctgtggtt gaagccggag actgggacag
      421 cacattgtgg atgattggcg gccctgtctt cttctcgctg ttagcgatgg cgatttacca
      481 gaactattgc agtcgcatgg cgaataaacc tgagttagct ctcgactgat catatgaaat
      541 cctgcaactc atcgtctctc cggcgatgag ttgtcaggaa gtaatgcgta tggatgatac
      601 tctccagggc cggtgataac tgaagatgat gttaagctgc taaatgagcc acgttgacat
      661 catttggcag cttcatgcat tacaaacttc ccgcctgacg acctttgccc gcagacgtca
      721 gcgttctccc cgtttgttta ccgttgaggc tttccagacg catctggaag ggcgggaatg
      781 gcatatcgat accgtgggcg tggaagcctg ccagaatcag ctggtggatc tcatggcgta
      841 gcggcatacg gtgacccatc tcagcggcgt aaatacgcag ctcgaaaatc tgaatcccct
      901 gttgcagatc caccaggaag acttccggtg ccgggttgtc gatcaccagc gagcagcgac
      961 gcgctgcggt gagcaggatt tccgtcactt cttcactgtt ggcatcggca ggggccggaa
     1021 tcgtcaacac tacgcgcgtg accgagtcag agagcgacca gttgataaac tgctcggtaa
     1081 taaacgcctt gttcggcacg attatctctt tacggtccca gtcgctgatg gtggtggcgc
     1141 gggtgttaat ttttgtcacg ctaccagtaa gatcgcggat cgttaccgta tcgccaatgc
     1201 gaatcggttt ttcgaacagg atgatcaggc cagagataaa gttggcgaaa atttcctgca
     1261 aaccaaaacc gagaccaaca ccgagcgcgg caaccagcca ctgcaatttc gaccactcaa
     1321 taccaatcat tgagaagccg accagcccgc caatcagcat cagcagatat tttgtgatgg
     1381 tggtgatggc gtaacccgta cccggcgtta aatccaggtg ctgcaaaatc gccagttcca
     1441 gcagcgcggg caggttgcgc accagctgcg tggtgatgat aaataccaga atggcaatca
     1501 gcaccgcacc gagggtaatt ggctccagac tttctacgcc ctgtaccgtg gaggtgacat
     1561 cccacagcga aatattttcg aggaagccga aagcggaatg gatttctgac cacagcacaa
     1621 tgaccgaaag cagggcgatc aacatcaaaa ttgagcgcac cagccgcaag gattgcgcac
     1681 tgatggcatc gagatcgact tcgctttcat cgacttcaat tgctccttcc gggctactgt
     1741 gatgatgcgc ttcctcttcg ccacgcgcac gttgcgctaa catctctgcc cggcgatgct
     1801 tcgcccggtc aaacgccagc ctgcgacgct ggatcagcat ccagcggcgg ataacgtgat
     1861 aaaccaccag tagcaggaac cagatggcaa ccgaggtttc aagcctcgct aacagtgcct
     1921 gcgccgttgc cagataaccc accgccgacg ccagaatggc aaccagtggt gcgccaatca
     1981 tcatgttcca cagcatatgg ttggtaatgt tgtcgccgct gccttctttg ttgagataca
     2041 acggaatacc ggcttttttc aggcttaggg tgaccaccgc cagcgcaccg caaatgagga
     2101 taaagcaaag ccgtcccagc gaaccggaaa attcgcggtc gtcgaggtta tcgaacatca
     2161 tcagcgccat aatcagcccg atgctcatca ggtagtagcg catcccacgg gaaacacgtt
     2221 cgcgcggcca gccaaaatga gcgataaaca agccgttcgg gcgggcaaag gtggcgcaaa
     2281 tcatcactac ccacagcagc ggcacggtgg ccgttacacc atcgccaatc gcgaccgcca
     2341 gcggataagg ccacgcctcg cgcaagccgt aacccagcgt catccacagc accggtaacg
     2401 gtgacgcgac gagaatcgac cagaaaagag tgcgcaacgt cagccagaag tgatcctgag
     2461 tcactttgcc gactttcgcc gccgaacgtt caagaaaacg ggtgaaatag cggcgcgagt
     2521 aaatactgca accgaccaga atcaacgcgc caaacagcgg caaaattgtc tctttgctgg
     2581 tcagcatcat cacactggct ttgcccaact gactgaaggt gtccagcgaa atgagacgac
     2641 gcagatcctg ggcgatttcc agcggccagg cgatggtcat cgggcgcacg tcagaggccc
     2701 agaacagata gcggtgcgtt gcttcgttca cctctttcag cgcatcctcc agttgcctgt
     2761 tggagacttt cagcttggtc agttccaata gtagcgtgtc gccaccctgc aacaatgagt
     2821 tcagcaactc acgctgagtg cgcagttgtg cttccagaat acggttttgc tcggcggtca
     2881 gcggctgacc gtcggcctga tggatttgcc gtagcagcgg ctgtttatta agtaaatcct
     2941 cataacgtaa ccgttgcaca cgcaactgcg ccatttcggt atcaagctgt tgtggttttg
     3001 gcatttctgg tagccgtgcc acctgcgccc gcagcgcttc gccgagcagg ttggacgatc
     3061 ccagccattg cgactgttca cgcagcgtat tcaacgcctg ccggacctgt aacgtctggc
     3121 tggcagcctg acgctgttgc gaggcaacga gctccatccg ctgcgcctgt tgattcagcg
     3181 ctgccgatag ttcgcggtta attttgaatt gcgcgacgat atctttcggc aaatcggcac
     3241 tgttttctgc cagtaattcg gtactttcca gcgcccgctc cgcctcaagc tgacgttggc
     3301 tattcaactg attacgtaag gcctgcaaat acgcatccag ttgctggctc tctttttccg
     3361 ccagctctga gcgtaagcgc gctaattcct ggcggttatt ggcagacagc tgcgccagct
     3421 ccagttcatc aacgagcgcc ttaagacgtg cagagtcaga ctgcaacgcg aaattttgtg
     3481 cctgattgag cggagtattg ccggtaagcg ttcccaggcg gcgctcgatc tcatttaact
     3541 gacggcgggc gtcggtttgc tgttgcggca gttgattcag cgaatcggca atctcgcggg
     3601 cgcgctcctg ctcttgctgg gcctgacggc ttttatccag caactggctg ctgacctgga
     3661 gaatttcctg attcagcgcg tcggtagaca ttcccggcga cacgctgcgc ggctcgtcac
     3721 gaatgttgtt taattgtgcg cgcagagtag cggagagttt cggataatta tcgataactt
     3781 gctgatattg tttgatgcgc tcaagggaac cttttcgttc ctcaagcgca tttaaggcag
     3841 actggagcgc ctctacgact tccggctgtg cgggtttcgc cgcttttgcc tgctccagtt
     3901 cctgagtgat ttgtttgcta tcgggggccg tcgcggcgta cgccccccaa ctgaggcacc
     3961 aggccatcag aaaagtgata atcaggcgca cgtcagcgtt tcctttgatg gattagacct
     4021 ggtctttttt atcgtcaacc aatgggctgg cgtcgtgttc tgcttcgatc tcttcagcag
     4081 gaagtggggc gggttcagcg tctggcgtaa caaaggtttc ggtagatact gccagcggct
     4141 ggccaatttt cgtgacagac aggctttcca gttgctcaac cagattcact ttacccggtg
     4201 caaacaggtt gataacggtg gaaccgagtt taaagcgacc catttcctgg cctttcagca
     4261 gtgccacaga accgtcgttt tccccggcag gccaggtcca gcgcttgatg ataccttcgc
     4321 gcggcggcgt aatggtgccc gcccagaccg tctcaatgct gccaacaatc gtcgctccga
     4381 ccagaatctg cgccattggg ccaaattcgg tatcgaaaag gcaaatcacg cgttcgttac
     4441 gggcaaacag atttggcacg ttctgagcgg tgagatggtt aacggaaaag agatcgcccg
     4501 gcacgtagat catctcacgc agaataccgt tgcacggcat gtgtacgcga tggtagtcac
     4561 gcggggagag gtaagtggtc acaaacgtac cgttgcggaa caggtccgcc atcagatagt
     4621 tgcctgccag cagggcttcg aggctgtagt tgtggccttt ggcttgcagg attttatctt
     4681 cttcgatttt acccaactgg ctgataacgc catcggcagg catgaccagt acattcggat
     4741 cggtatcgat tgggcgtact tcgtcacgca gcggacggac aaagaattcg ttaaaggtgc
     4801 ggtagctggc ggtgtccggc ttttgcgcct ctttcatgtc gaccttgtag tatttaacga
     4861 acagatcgat aaccagtttt gtcagccatc ctgcccgctt gcttgcgccc caacccgcca
     4921 ggcgagtaag ccatagtttc ggcagaatgt actgtagcga aagtttaaat gaatttaaca
     4981 aggtagcctc caggccattg ttttgtcgtt cctgatccgg ccaacatgcc ggatcctgaa
     5041 aaaaagggga cgattctaac gacggttagc ttaattgtca gtcatccgta tcagaaaagt
     5101 ttttacgcgt ttttacctgc gccatgcttt ccagaatacg gtgatagttt tcgaaacggg
     5161 tttccgcgat tttcccttcc tcaaccgctt cccggatagc gcagcccgga tcggtatcgt
     5221 gtttgcaatc gcgatattta cacagaccta aatagtcatg gaattcgaca aagccctgag
     5281 tgatttgttc cggctccagg tgccagaggc cgaactcacg cacaccaggg gaatcaatca
     5341 catcaccgcc gtgcgggaag tgatacagcc gagcggcggt cgtggtgtgc tgaccgagcc
     5401 ccgagttgtc agagacatcg ttggtcagga tctctttttg cagccccagc aacgcgttca
     5461 gcaggctgga tttaccgacg ccagactgcc cggcaaaaat gctgatgcgc ccggtcaacg
     5521 cctcttccag cggtttcagc ccatcctgag tatggctgga aaccatcaat acgcgataac
     5581 cgatgttgcg gtagatatcc atctgctcgt tgacgaacgc catgccttcg tcgtccagca
     5641 ggtctatctt gttgagcacg ataatcggct caatctgcaa ggtttcgcag gccaccaggt
     5701 aacggtcgat aatattgagc gacagctccg gcaaaatggc ggagacaatg acaatctggt
     5761 cgatgttggc ggctatgggt ttcacgccgt cgtagaaatc cggacgcgtt aacaccgagg
     5821 tgcgctcatg caccgcttcc acgatccctt tgacatttac gccttccgcc gccggtttac
     5881 ccggacgcca gactacgcgg tcgccggtta ccagcgaacg gatggtacgg cgaatattgc
     5941 agcggtgaac gtcgccatcg gcggattcca catcagcgtg cataccaaag cggctgatga
     6001 cgataccttc atcaggctcg ccaaacagat tgtcgtcgta gtcgggcttc tccttagacg
     6061 ttttaagacg acgctggtga ttggcgttca cgcggcgctg ctggcctttg gagagtttat
     6121 ttttactcaa tcgtactgac tcctggtcgc ccctgatggg caaaacatct atgatacacg
     6181 caattgtgga tcaatatagt cactgtgaat gggtggaaaa tagcatgagt gccaatgaaa
     6241 acaacctgat ttggatcgat cttgagatga ccggtctgga tcccgagcgc gatcgcatta
     6301 ttgagattgc cacgctggtg accgatgcca atctgaatat tctggcagaa gggccgacca
     6361 ttgcagtaca tcagtccgat gaacagctgg cgctgatgga tgactggaac gtgcgcaccc
     6421 ataccgccag cgggctggta gagcgcgtga aagcgagcac gatgggcgat cgggaagctg
     6481 aactggcaac gctcgaattc ttaaaacagt gggtgcctgc gggaaaatcg ccgatttgcg
     6541 gtaacagcat cggacaggac cgtcgtttcc tgtttaaata catgccggag ctggaagtct
     6601 acttccacta ccgttatctc gatgtcagca ccctgaaaga gctggcgcgc cgctggaagc
     6661 cggaaattct ggatggtttt accaagcagg ggacgcatca ggcgatggat gatatccgtg
     6721 aatcggtggc ggagctggct tactaccgcg agcattttat caagctgtaa aatttttcct
     6781 ggtcacgtaa gcgccggaat ccacttgcgg cggcgtgatt ttgacgctaa atcgaacgtc
     6841 ttgctgataa tttcagcact tgagataaaa acgcaaaaaa aacttttttg gggggttgca
     6901 gagggaaaga tttctcgtat aatgcgcctc ccgtaacgac gcagaaatgc gaaaattacg
     6961 aaagcaaaat taagtagta
//
LOCUS       DAJTYM010000155         6978 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTYM010000155 DAJTYM010000000
VERSION     DAJTYM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..598
                     /locus_tag="OO575_003693"
                     /pseudo
     CDS             1..598
                     /locus_tag="OO575_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            603..1568
                     /gene="rbsC"
                     /locus_tag="OO575_003694"
     CDS             603..1568
                     /gene="rbsC"
                     /locus_tag="OO575_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6666495.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            1593..2483
                     /gene="rbsB"
                     /locus_tag="OO575_003695"
                     /pseudo
     CDS             1593..2483
                     /gene="rbsB"
                     /locus_tag="OO575_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            2609..3538
                     /gene="rbsK"
                     /locus_tag="OO575_003696"
     CDS             2609..3538
                     /gene="rbsK"
                     /locus_tag="OO575_003696"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6666496.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDKIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            3542..4534
                     /gene="rbsR"
                     /locus_tag="OO575_003697"
     CDS             3542..4534
                     /gene="rbsR"
                     /locus_tag="OO575_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6666497.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(4500..5927)
                     /gene="mdtD"
                     /locus_tag="OO575_003698"
     CDS             complement(4500..5927)
                     /gene="mdtD"
                     /locus_tag="OO575_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6666498.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(5950..6642)
                     /locus_tag="OO575_003699"
     CDS             complement(5950..6642)
                     /locus_tag="OO575_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6666499.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1772 a         1696 c         1842 g         1668 t
ORIGIN      
        1 cgcgcaccag cggttacgtc accctggatg ggcatgaagt cgttacccgt tcaccgcagg
       61 atggcctggc aaacggcatt gtgtatatct ccgaagaccg taaacgtgac ggtttagtgc
      121 tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct gcgctacttc agccgcgctg
      181 gcggcagttt gaagcatgcc gatgaacagc aggctgtgag tgatttcatt cgtctgttta
      241 atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct ttccggtggc aatcagcaaa
      301 aagtggcgat tgcccgcggt ctgatgacac ccccaaagta ttgatccttg atgagcctac
      361 ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa ctgattaacc agttcaaagc
      421 cgatggcttg agtatcattc tggtgtcatc ggagatgcca gaagtattag gcatgagcga
      481 tcgcatcatc gtcatgcatg aagggcatct cagcggggaa tttactcgtg agcaggccac
      541 ccaggaagtg ttaatggctg tcgctgtggg caagcttaat cgcgtgaatc aggagtaaaa
      601 aaatgataac ccagactgtc tctggtcgcc gttatttcac gaaagcgtgg ctgatggagc
      661 agaaatcgct tatcgctctg ctggtgctga tcgcgattgt ctcgacgtta agcccgaact
      721 ttttcaccat caataactta ttcaatattc tccagcaaac ttcggtgaac gccattatgg
      781 cggtcgggat gacgctggtg atcctgacgt cgggcataga cttgtcggta ggttctctgt
      841 tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat tgaagtcaat gcgctggtgg
      901 ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc ggtaaccggg gtgattgtag
      961 cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat gatgctttta ctgcgcggcg
     1021 tgaccatggt ttataccaac ggtagcccag tgaataccgg ctttactgag aacgccgatc
     1081 tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc gacgccagtc tggatcatgg
     1141 ggattgtctt cctcgcggcc tggtacatgc tgcatcacac gcgtctgggg cgttacatct
     1201 acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg tatcaacgtc aataaaatca
     1261 aaatcatcgt ctattctctt tgtggtctgc tggcatcgct ggccgggatc attgaagtgg
     1321 cgcgtctctc ctctgcacaa cccacggcgg ggactggcta tgagctggat gctattgctg
     1381 cggtggttct gggcggtacg agtctggcgg gcggaaaagg tcgcattgtt gggacgttga
     1441 tcggcgcatt aattcttggc ttccttaata atggattgaa tttgttaggt gtttcctcct
     1501 attaccagat gatcgtcaaa gcggtggtga ttttgctggc ggtgctggta gacaacaaaa
     1561 agcagtaata acgactacag gacatcttga atatgaacat gaaaaaactg gctaccctgg
     1621 tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc gatggcaaaa gacaccatcg
     1681 cgctggtggt ctccacgctt aacaacccgt tctttgtatc gctgaaagat ggcgcgcaga
     1741 aagaggcgga taaacttggc tataacctgg tggtgctgga ctcccagaac aacccggcga
     1801 aagagctggc gaacgtgcag gacttaaccg ttcgcggcac caaaattctg ctgattaacc
     1861 cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc taactaggcg aacattccgg
     1921 ttatcactct tgaccgccag gcaacgaaag gtgaagtggt gagccacatt gcatctgata
     1981 acgtactggg cggcaaaatc gccggtgatt acatcgcgaa gaaagcgggt gaaggtgcca
     2041 aagttatcga gctgcaaggc attgccggta catccgcagc ccgtgaacgt ggcgaaggct
     2101 tccagctggc cgttgctgct cacaagttta atgttcttgc cagccagcca gcagattttg
     2161 atcgcaccaa aggtttgaac gtaatgcaga acctgttgac cgctcatccg gatgttcagg
     2221 ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct gcgcgcactg caaactgccg
     2281 gtaaatcgga tgtgatggtc gtcggatttg acggtacacc ggatggcgaa aaagcggtga
     2341 atgatggcaa actagcagcg actatcgctc agctacccga tcagattggc gcgaaaggcg
     2401 ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca ggctaagtat ccggttgatc
     2461 tgaaactggt tgttaagcag tagttttaat caggttgtat gacctgatgg tgacataaat
     2521 acgtcatcga cagatgaacg tgtaatataa agaaaagcag ggcacgcgcc accctaacac
     2581 ggtggcgcat tttatggaca tcccgaatat gcaaaacgca ggcagcctcg ttgttcttgg
     2641 cagcattaat gctgaccaca ttcttaatct tcaatctttt cctactccag gcgaaaccgt
     2701 aaccggtaac cactatcagg ttgcatttgg cggcaaaggc gcgaatcagg ctgtggctgc
     2761 tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact ggcgatgaca gcattggcga
     2821 gagcgttcgc cagcagctcg ccactgataa aatcgatatt actccggtca gcgtgatcaa
     2881 aggcgaatca acaggtgtgg cgctgatttt tgttaatggc gaaggtgaga atgtcatcgg
     2941 tattcatgcc ggcgctaatg ctgctctttc cccatcgctg gtggaagcgc aacgtgagcg
     3001 tattgccaac gcgtcagcgt tattaatgca gctggaatca ccactcgaaa gtgtgatggc
     3061 agcggcgaaa atcgcccatc aaaataagac tatcgttgcg cttaacccgg ctccggctcg
     3121 cgaacttcct gacgaactgc tggcgctggt ggacattatt acgccaaacg aaacggaagc
     3181 agaaaagctc accggtattc gtgttgaaaa tgatgaagat gcagcgaagg cggcgcaggt
     3241 actgcatgaa aagggtatcc gtactgtact gattacttta ggaagtcgtg gtgtatgggc
     3301 tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg gtgcaggctg tcgataccat
     3361 tgctgccgga gataccttta acggcgcgtt aatcacggcg ttgctggaag aaaaaccatt
     3421 gccagaggcg attcggtttg cccatgctgc cgctgcgatt gccgtaacac gtaaaggcgc
     3481 acaaccttcc gtaccgtggc gtgaagagat cgacgcattt ttagacaggc agaggtgacg
     3541 cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt tctacctcaa cagtttctca
     3601 cgttatcaat aaagatcgct tcgtcagtga agcgattacc gccaaagttg aagcggcgat
     3661 taaagaactc aattacgcgc catcagctct ggcgcgtagc ctcaaactca atcaaacaca
     3721 taccattggc atgttgatca ctgccagtac caatcctttc tattcagaac tggtgcgtgg
     3781 cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc ctttgcaata ccgaaggcga
     3841 tgaacagcgg atgaatcgca atctggaaac gctgatgcaa aaacgcgttg atggcttact
     3901 gttactgtgt accgaaacgc atcagccttc gcgtgaaatc atgcaacgtt atccgacagt
     3961 gcctaccgtg atgatggact gggctccgtt cgatggcgac agcgatctta ttcaggataa
     4021 ctcgttgctg ggcggagact tagcaacgca atatctgatc gataaaggtc atacccgtat
     4081 cgcctgtatt accggtccgc tggataaaac tccggcgcgc ctgcggttgg aaggttatcg
     4141 ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc tatgaagtca ctggtgattt
     4201 tgaatttaac ggcgggtttg acgccatgcg ccaactgtta tcacatccgc tgcgtcctca
     4261 ggccgtcttt accggaaatg acgctatggc tgttggcgtt taccaggcgt tatatcaggc
     4321 agagttacag gttccgcagg atatcgcggt gattggctat gacgatatcg aactggcaag
     4381 ctttatgacg ccaccattaa ccactatcca ccaaccgaaa gatgaactgg gggagctggc
     4441 gattgatgta ctcatccatc ggataaccca gccgaccctt cagcaacaac gattacaact
     4501 tactccgatt ctgatggaac gcggttcggc atagatttac gctgtctttt gatcaaatta
     4561 ttaccatcgg ttgttttcag aagcatgaac attgctgctg aagcaacagt aataatgccc
     4621 atcgtgataa acgtatagtg gaattgttcg acagtcgttg tgccttccat cccttcataa
     4681 atgcgaagga cggccgcact tacagcaacg cctaaactaa tcgacagttg ctgcgtgacc
     4741 gccagaacac tgttaccgct gctggcgttg tcatcggtca gatcggcaag tgtgatggta
     4801 ttcatcgcgg taaactgcgt cgacatagcc atccctaata taaacaacgg caagatcagc
     4861 atccatatcg ccattgccgg tgattgcaaa gagaactgag cgatcattag cccaataatc
     4921 accgtgatcc ccactaatgt atggcgatag cccagacgac gtaagacttg ggtaaccatc
     4981 gattttgcaa taatggaacc taacgctgtc ggtgccatca tacagccggc aataaacgcc
     5041 tgataaccaa atcctacctg taacatcaat ggcataagga acggtacaca gccggtcccc
     5101 agacgggttg caatattgcc tacgataccg atcgagaaag tgcgggtttt aaataaatct
     5161 aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa tatagagaag cagtaacccg
     5221 atgctggtga caattaccgt caaggcaatc cagctggcga caatcttttc cccgaatagc
     5281 tctattccgc ttgaaaagag aacaaggctg aggccaaaca gcaaaaagcc agtgatatcg
     5341 aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc gcgcgtaaag aaggcccgca
     5401 atacctatgg ggatattaat taaaaatatc cagtgccagg ttgcccaggt gaccagcacg
     5461 ccgccaagaa cggggcctaa aattggcccc accagacccg gcatggcgac aaaattcaat
     5521 accggaagaa gttcattacg aggataagca cgcagtaagg ccggccgagc aacaggcatc
     5581 atcattgcgt cgcctatccc ttgaataacc cggaagacaa ccagctgtgg tagcgaatta
     5641 gaaagtgcgc aggccagaga acccaacgtg aacagactca cggcaagggt aaaaatgcga
     5701 cgcgtaccga agcgatcggc tagccatccg cttaccggaa taagcatcgc caccgtcagc
     5761 gtataactga tgatggctga ttgcatcgcg agaggagaac gattaaggct atgagcgatt
     5821 gcgggtaagg cggtattcag aatagtggca tcaagtgcct gcatgaagaa ggccatcgcc
     5881 gcgatccacg gcaaacccga catactacgc ttctttttat cgctcattca atgtcctgtt
     5941 atcgggttat cacttatcag gtgagcgtag cagcgcctga caagctttaa atgccgcgtc
     6001 gccatcgctt tggataatcg catcgacaat cgcctggtgc agatccagct ttatcactgt
     6061 gtcgctggta attgacgtga agtaagtgtg ataaaccgaa tggaatagcg aggcgaatga
     6121 ggtcaaaaac ggattggcgc tcatttcata gatatgctca tgccaggcca tatcgacttc
     6181 gatccagcgt tcacggcgaa agttctcttt taatgccgcc atttcggcca ttaacgtatt
     6241 gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc agtaggcagg cttgcggctc
     6301 cagacagata cgcataacca gaaagtgatc gatgacctga tgaaagttct cttctgttat
     6361 ccaccaagta agcaattcct gatcaagaaa attccagttc gattgtggca tgacccgagt
     6421 accaattcgc ggtcgcggta aaaccatccc ttttgccgtt aacgttttga ccgcttcgcg
     6481 taccgctgta cgactcactc caaattgctc gcccagctca atctcaccgg gcaaaatggt
     6541 gccgggttca tattcacctt ttaagatccg ttgcgccagc ttctcagcca gaacatacga
     6601 aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc attacttatc ttcctttttc
     6661 tttttattcc tccttagtat gccaccagga agtgtgatta cggttgcaaa aacggcaaat
     6721 tgcttgtttt atggcacatt aacggggtgt tttgctgaaa aaatgcgcgg tcagaaaatt
     6781 attttaaatt tcctcttgtc aggccgggat aactccctat aatacgccac cactgacacg
     6841 gaacaacggc aaatacgccg ccgggtcagc ggggttctcc tgagaattcc gacagaaaaa
     6901 gcaaaaataa atgattgact ctgtagcggg aaagcgtatt atgcacaccc ccccccgcgc
     6961 cgctgagaaa aagcgaag
//
LOCUS       DAJTYM010000156         6963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTYM010000156 DAJTYM010000000
VERSION     DAJTYM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(365..946)
                     /locus_tag="OO575_003700"
     CDS             complement(365..946)
                     /locus_tag="OO575_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666500.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(993..4850)
                     /gene="sigA"
                     /locus_tag="OO575_003701"
     CDS             complement(993..4850)
                     /gene="sigA"
                     /locus_tag="OO575_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6666501.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5211..6215)
                     /locus_tag="OO575_003702"
                     /pseudo
     CDS             complement(5211..6215)
                     /locus_tag="OO575_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6200..6577
                     /locus_tag="OO575_003703"
                     /pseudo
     CDS             6200..6577
                     /locus_tag="OO575_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6616..>6963)
                     /locus_tag="OO575_003704"
     CDS             complement(6616..>6963)
                     /locus_tag="OO575_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6666502.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1734 a         1568 c         1573 g         2088 t
ORIGIN      
        1 catcacctgc tattaatccc cgagtcagta cttcgcggcc tgacgcgatc accctactca
       61 gtacttcaga gcccgcctcc ggtacctgcc tggcgatcgc ccctgtgcaa caaaataaat
      121 caaccgttat caaaccacga tcgttcgtat gcgccgccga tgacgcgcgc ctgaaaaacg
      181 cgtcaatatg agttccccgg aagcttacga taatacgctt atgaacatta caacacgctc
      241 atgcggaacc tggatggggg aaggaacggt gaagctgtta gcgaatgacc ggcgtttata
      301 caaacgccgg tcatgatgaa tgatatttca gaatatacct cgggctaaaa taccgggaca
      361 gagatcaggc gtgattaatc tgcagcattt ttaataacag aagtactgag cttactttac
      421 ctttgatgat gtaatagtta tttccgtcgg tactcatttt tccctgaaat aatgtcacgg
      481 atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt catattttta atgagctgag
      541 atccgggtgg caaggggtat ttgctgtcag atactgggcg actgagtctg atgtggcgcg
      601 tatacagccc gttttcgtac agggtggcct ctgttatggt gtcagtgacc agcatgtggg
      661 agacaatgct gtattctttt ttaaagcacc caccagaaac ggtgattgta atgataatgg
      721 tcttctcgat cgagatgtaa atacccgttt cgttatactg tccgtatcgg aaccatgaga
      781 actcagttgc attccagcca tgctccagaa tatgtgcatt tttttgcctg atagtttctt
      841 tttttattcg ctcatgttta aggcataaca ggcagataaa taatgtcaga aaaagaaagc
      901 taataatgag agcaagagcg atatagtctg tcacagggta atacatattc tctccggtag
      961 aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag agtaacggaa gttggcgttg
     1021 atcgcgttat ccacgttgta tttaccaaat gccgatttct caaactcaag accgaagcga
     1081 agattatcgc gaatttcggc gttgagacca acattcatga gaatacgacc gtctttttca
     1141 cctttgatac gtttctcacc ggacgcatca cgcagtacgg tttcaccgtt ggcaaacagg
     1201 tcaaactggt agccaaggcc ggcgcgggct gtgactttcc agtccttacc ggagaaggat
     1261 ttacccacat caacaccggt acgcccaatc agcggattaa agtccttatc cttcatggtc
     1321 acgctcattc cgcgatcctg ccagtcgaac cgtttacctg atacggcccc ataaaccagt
     1381 tctgcctgcg gctcaatcca ggagtcttct gttacatgat agcggtagcc tgcttcagca
     1441 cccacataca aggaatgaga actgtagtct tttgttccga gtccagcaaa ggtcgccgag
     1501 tactcattat catggtgaac atacttacta atcaggtcga tataggcacc agagtcaaat
     1561 atagcggaag catacagacc tgccccgacg gacttcgtct tgccactgaa tgcattactg
     1621 ctggcatgac tgtcggtata cgtcatagtc agaccagtga aaaggtcaag tccatccagc
     1681 tcatgttttt tatccacacc aatctgcaca tgggtgtagt tgtcactgta tccaccagaa
     1741 gctgaacctg ctccgctcat gatgcgggcc catgcaccgg cctcgccatt ggtgtcacgc
     1801 agatccccca ttcgtttatt caggttgttg acttctgtca ggaaggcttt gtaattaccg
     1861 gacatatacg attttgctgc accggaagat gctgtatttt cagccacctt atatccggtc
     1921 agggtccacg tggtatgttc ctgatcctct ttcgtgctga tcacaggaga tatgttgctg
     1981 aaacctatgg cctgagtact ggctttaaat atctctttat tgcttccttt tggggctgtt
     2041 atcaggtcaa cgtttaatcc tgagttatta tttcccactt tctgtatatc gaccgacaga
     2101 ctgttattgc ttccggttaa ctgattttta acaatgagtt tatccgattg atttacagac
     2161 tggctcagat cagcccgcat aatcacagca gtattagtgg catccagtgt gtttactgtc
     2221 acagtatgga aacccgaatt ctctatgtta ctaaacaaca aaagactgtc gcgggttttt
     2281 aatgattgca gggctgactg tccgtttact ctccataagg tgttatacat gctggctgag
     2341 gctgcaggag catcaatacc tccggtataa gaagtatcaa agccacccag caaacctgta
     2401 gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt tatactggtc aacaccaaac
     2461 agaatagatg cttttttgtc tgattttata ttaccattga cccaggcata attttgagcc
     2521 tttaacgtgg caccatcttc tgtcatatta tatccatctg acatatagat tgccgggctg
     2581 tatttattat tttctgtagg ggtgcctgtt aatgtcaggg ttccggccag ttcaatagct
     2641 ttatctacat acatttttcc ctgggcggta acagtagctc ctttgttgat aatcaggtct
     2701 gtattaatta aggataatgc accagtcata gtaacagttg atttatcacc aacagttatt
     2761 ttgctgtttt gagcactcag actgccgttg aaactacttt cgcctgctga atccccggag
     2821 cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt ttcttcctga gaacatgtca
     2881 atgtatgcag ttttatttcc cagaacaatt tgagagtttt tagcctctat gtgtcctttt
     2941 acattagcat cccggcctat actcagggtt gcgttttcta aatttaattt ccggaaatta
     3001 aatttacgac tttcccagtc gggctgggaa aaagaggcta tctgattgtt ggacttatat
     3061 tccgtattgt aacgtttatt tacagtgctt tcctggtcct ttatggcagc agaatagtct
     3121 ttttgacata aaaaaggaat tggacagttg ttattgcctt ctttaaatat ggcatgttct
     3181 gtcgcatgtc cttgtaatgt taactgtgca ttccggacag agatatcgtt tttgatatcg
     3241 actgagccat caaagatcag attggcatta ttgttatgtt gctgggtagt attgataata
     3301 tgatttatgc ttatattacc tgatacattc ccatggtaca tatatgcatc agtattagta
     3361 agtgataatg ttgattgctt cacgtttgag ttcgttacgg ttgttcccgc atctgttgct
     3421 gcgattttct gaaatgactg gtcatagccg tttagatcca gtgttccgcc atttctggta
     3481 aaatagattt cgccattacc gctttcgctt aaagcgtctt tggcatttat ttttaccgta
     3541 cctttacctc cggccatgta aattttattg aaggcttttt cagcactaag gatgacagtc
     3601 ccattaccta ttttaaggtt atttccctgt gctattttta catccagagt accactacct
     3661 attttatgca gattatctcc ggcaacgccc ttaacattcc attcaacggt ggtattatta
     3721 tcaatatcta tcccggcacc tttataagac ttatcttttc cgtacacttt atatgtctgg
     3781 ttcttatcga atataagccc gccaatgcca agatcggtat cctgcttgag cgttaatacc
     3841 cctcctcctg agacagacag gtccttatct tttataatcg atgataactc ttgtttttta
     3901 ccatccagct gcatagtgtt tgtgaaattc ttttttaaat tgtttaccag aatattatta
     3961 aataacgagt agtttgtcca ggctatattg gtactactga taaaaccaac tgtactggta
     4021 acaccatgga aaacccattt ttgttgttct ttatcatata gataagaacc agaaccacta
     4081 tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg tataaatgta actcttgttt
     4141 ttccagtcca gattaaaaag acttgcgctt aacaagtcgg gattatagtt ttgtccaaac
     4201 agatattgtt tgccgtttaa tatcgttgag gttgttccgg cacctgctct gaagccaatt
     4261 atttgttcct taccgttgta gtttacgcca tatcttttca tcgcatcttc gggagaaagt
     4321 gagaagtcgg cactctccgt aacccctgtt gtttccacga cgaatttatt cagacgatga
     4381 accgaaaagt cgccagatga tactctgtta cttgctttat atgctgtctg atcccatgac
     4441 tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg cagtaacact gtacgcacct
     4501 ccaattgatg ttgttgcacc tttgttggaa acagcagaaa aatcaggtac aggtagttct
     4561 ggaaaatgaa atatctttcc atctttcatc attaattgaa catttgttgc acccggtctg
     4621 aactctcctc tgttttgagc aaggtcaaga taatcgcggg tccatatatt atttatatcc
     4681 agcattgctg caaatgctgt ttcgccataa cttccatata ttgaggataa aataataccg
     4741 aggataactt tctttcttga tgtaccgaca ctaactttcc gggtcagttc agaaacagca
     4801 actaatccac ctgtaatatg actatatttc agtgaataaa ttttattcat acgtgaactc
     4861 cgatatttct gagttgtggt tatgggatag catttaatta caacggggaa ctatcagtca
     4921 tggtacgtaa ctgataaggg tataatgata atcactgtca gttatatttg tatgacagat
     4981 gtaacaaaat gaaaaaaaag ataagcacaa gtgattgatt ggttagatat tgatcacttg
     5041 gtgtttgtat tttgaatttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     5101 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     5161 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     5221 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     5281 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     5341 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     5401 gtgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     5461 tccacatcaa atgcagtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     5521 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacgggag
     5581 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     5641 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     5701 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     5761 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     5821 tcagtgatca gtgccgtgcg ggcacaggcc tgtgatgtca ggcggctttt gtccacctcc
     5881 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     5941 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     6001 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     6061 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
     6121 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     6181 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     6241 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     6301 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     6361 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     6421 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     6481 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     6541 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     6601 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     6661 aatatcccac ataaatccag tcaactcacg agccaccgcc gtaatggcta cttgtgattt
     6721 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     6781 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     6841 ttttgcaggt gtacgataag accaggcggc ttcgtaaagc agacgtctca gttcgctgtt
     6901 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     6961 cag
//
LOCUS       DAJTYM010000157         6935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTYM010000157 DAJTYM010000000
VERSION     DAJTYM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..712
                     /gene="ydfJ"
                     /locus_tag="OO575_003705"
     CDS             <1..712
                     /gene="ydfJ"
                     /locus_tag="OO575_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6666503.1"
                     /translation="KVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTF
                     LAGYLVQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLA
                     WPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKE
                     IGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALED
                     AAEDQPRVVRAAQPSRSL"
     gene            801..2261
                     /locus_tag="OO575_003706"
     CDS             801..2261
                     /locus_tag="OO575_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6666504.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2297..2500)
                     /gene="ydfZ"
                     /locus_tag="OO575_003707"
     CDS             complement(2297..2500)
                     /gene="ydfZ"
                     /locus_tag="OO575_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6666505.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2678..3364)
                     /gene="ydfH"
                     /locus_tag="OO575_003708"
     CDS             complement(2678..3364)
                     /gene="ydfH"
                     /locus_tag="OO575_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6666506.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3453..4199)
                     /gene="ydfG"
                     /locus_tag="OO575_003709"
     CDS             complement(3453..4199)
                     /gene="ydfG"
                     /locus_tag="OO575_003709"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6666507.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4336..6381
                     /gene="dcp"
                     /locus_tag="OO575_003710"
     CDS             4336..6381
                     /gene="dcp"
                     /locus_tag="OO575_003710"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6666508.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6426..6923)
                     /locus_tag="OO575_003711"
     CDS             complement(6426..6923)
                     /locus_tag="OO575_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6666509.1"
                     /translation="MVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGGKLASALC
                     AAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAIERADADV
                     SIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAVRFALAQL
                     LQLLL"
BASE COUNT         1722 a         1656 c         1770 g         1787 t
ORIGIN      
        1 gaaggttaac gacagcaacc aaccgacagc aaaacctgca cctgctggta gcatgttcca
       61 gagcaaatcc ttctggctgg caacagggct gcgttttggt caggcgggta actccggttt
      121 aattcagact ttccttgcag gctatttagt acagacgtta ttgtttaaca aagcaattcc
      181 aacagatgca ttgatgatca gttcgattct cggctttatg accattccgt tccttggttg
      241 gttatccgat aaaattggtc gccggatccc gtatattatt atgaatacct ctgcgattgt
      301 gctggcgtgg ccaatgcttt ctatcatcgt agataaaagc tatgccccga gcaccattat
      361 ggttgcactg attgtgattc ataactgtgc ggtgctggga ttatttgctc tggaaaacat
      421 taccatggca gaaatgttcg gctgtaaaaa ccgcttcacc cggatggcta tttcaaaaga
      481 aattggtggt cttatcgctt ccggttttgg tcctatcctg gcgggtattt tctgcaccat
      541 gacggaatcc tggtatccga tcgccattat gatcatggca tattcagtga ttggtttaat
      601 ctctgcgctg aaaatgccag aggtgaaaga ccgtgattta agtgcgctgg aagacgctgc
      661 ggaagatcaa ccgcgtgttg taagagctgc gcaaccttcc agaagtctgt aaaccctcaa
      721 tcccttctct taccggagag gggattttta ttcatataac aaaacatata gcttgccata
      781 tttaaattta aggaattgtt atgggaaata atttgttatc agcaaaagcg acactccctg
      841 tttatgatcg taataacctg gccccaagaa ttgttcattt aggctttggt gcatttcacc
      901 gtgcgcatca gggcgtgtat gccgatattc ttgctacaga acatttcagt gactggggat
      961 attatgaggt caacttaatc ggcggcgaac agcaaattgc cgatttacaa cagcaagata
     1021 atctttatac cgttgcggaa atgtcggccg atgcgtggac ggctcgcgtc gttggcgtcg
     1081 ttaaaaaagc cttgcacgta cagatggatg gcttagaaac cgtgttggct gcgatgtgtg
     1141 aaccgcaaat cgcgattgtc tctctgacaa tcaccgaaaa agggtatttc cactctccgg
     1201 cgaccggaca gttaatgctc gagcacccga tggtcgctgc cgacgtgcaa aatccccacc
     1261 agccgaaaac agcaacaggg gtgattgttg aggcgctggc tcgccgtaaa gcggcaggac
     1321 ttcccgcatt taccgtcatg tcatgtgaca acatgccaga aaacggtcat gttatgcgtg
     1381 acgttgtcac ttcctacgcg caagccgttg atgtaaaact ggcacaatgg atcgaagata
     1441 acgtgacttt cccatcaaca atggtggacc gtattgtgcc cgcagtgaca gaggatacgc
     1501 tggcgaaaat cgaacaactt accggtgtgc gcgatcctgc tggcgttgcc tgtgaacctt
     1561 tccgccagtg ggttatcgaa gataactttg tagccggacg tccggaatgg gaaaaagcgg
     1621 gagccgaact ggttagtgat gtgctgcctt atgaagagat gaagttgcgc atgctcaacg
     1681 gcagtcattc attcctggca tatctgggtt atcttgctgg atatcagcac attaatgact
     1741 gtatggaaga tgaacattat cgtcatgcgg cgtatggcat gatgttgcag gaacaagcgc
     1801 cgacgctgaa agtgcagggc gttgatttgc aagattacgc taaccgatta attgcacgct
     1861 atagcaaccc ggcgttacgt catcgaacct ggcagattgc gatggatggc agccagaaat
     1921 tgccacagcg gatgttggat tctgttcgct ggcatctggc gcatgacagc aagttcgatc
     1981 tgctggcgct gggcgtcgcg ggttggatgc gttatgtcgg tggtgttgat gaacagggaa
     2041 atccgataga aatcagtgac ccactgttac ctgttattca gaaggctgta caaagtagtg
     2101 ccgaagggaa agcgcgcgtc cagtcattgc tggcgattaa ggcaatcttt ggtgatgatt
     2161 tgccagacaa tagcttgttt actgcaaaag tgacggaagc gtacttgtct ttattagcgc
     2221 atggcgcgaa agcgaccgtg gcgaaatatt ccgtgaagta aaaaaagcaa tgccgccatc
     2281 ggcggcattc acacacttag ttcatgccga gacgaatcag gtccagaggt gccagtttct
     2341 cttcacaacc ttcaacaact acggtttttc cgcggcggat ttgctcagcg gtcagacctt
     2401 cagtatcaat cgacaggatc ttgccagtgt gaccggtgcc gctaaccata acacggctgc
     2461 cagtggtgat tgcgttacgg ttacgatcgt aggtcgtcat aggtgttttc tcctttctga
     2521 tttacagtcg aagcccacgt aacgggcgca atataaatac tcctgtcgag tgaaatattt
     2581 tttgtttttg atcaagctca catctttttt gatgcatgca tgagccgtaa ggcgggggga
     2641 aaagcctctc atcccggatg ggatgagagt aaatgaatta ctcttcgcta aaccagtcgc
     2701 tgttttcctg gcggatctgg cgcacggatt cgctgatttc ctgcaaatgt tgcgtcattg
     2761 cacgttctac cgcatcgcca tcacgttttt gcagggcaga gaaaatatca agatgctggc
     2821 gtaacagcat ttctggtgga gaaacatggt cgaaactcat atagcgcacg cgatcaacgg
     2881 tcgctttcag gttctcaatg gtatcccacg ccagttgaca gtcggcaatc tgcgtcagga
     2941 gttgatggaa gttgtcatca agttcaaaaa aatcatccag ttgcttgcgc tcaatggcaa
     3001 tgcgttgctg gtgaagattt tgttccagtt ggtagcactg gctttcggta atcatgctcg
     3061 ccgcccgacg cgccaccgcg cactcaatgg cctgacggat aaaactgccg ttgcgcacct
     3121 gggccatgga aattttgttg acgtagctgc cacgctgcgg acgaatttga atcaggccgt
     3181 tttccgccag tttaataaag gcttcacgaa ccggctggcg tgacacattg aaacgaacag
     3241 aaacttcttt ttccgacaac ggtgtgcctg gtgcaatcag gcaatggaca atgtcgcgac
     3301 gaagaatacg ataaatctgc tgattgacag gctgtgtggg attaagttgc gtttcgacgg
     3361 tcattcgttc ttgcttgtga gtgagttaac tgcatgagtc taccactttt ttgtgacaag
     3421 caataacccg gcagttacgc cgggtataaa aattactgac ggtggacatt cagtccggca
     3481 tagctttggg taaccggcat catttccagg gtattgatat tgacgtgagc aggcagcgtt
     3541 gacacccacc agacggcttc gctgacatct tctggcgtca atgcaacggt attttgatag
     3601 gttttttccg ctttaccgtc atcgccttta aagcggacat tggaaaactc ggtgccaccc
     3661 accagacccg gttcgatgtc ggtgacgcgc accgccgtac catgcagatc cgtacgcaga
     3721 ttcaggctaa actgacgaac aaacgctttc gtcgcaccgt aaacgttacc accggcatac
     3781 ggccagctgc ctgccgttga gccaatgtta ataatatgac catgattacg ttcaaccata
     3841 cccggtaaga cggcgcgcgt catatatact aggcctttgt tgttggtatc aatcatcgtt
     3901 tcccagtctt caacgctggc tttatgcgca ggctccatgc ccaacgccag gccggcatta
     3961 tttaccagga tatcaatatt gctccactcg gcagggagcg atgccagcat ctcttcaata
     4021 gcggcgcggt tgcgaacgtc cagttgggcg atatacagat tatctcccag ttcgtctgtt
     4081 aactcctgca accgctcctg gcgacggcca gtggcgataa ctttatgccc ttgttgaata
     4141 aaacgacgag taatgcattc accaaaacct gccgttgctc cagttactaa aacgatcatc
     4201 tcactgttcc tcaacgcttt tgtgtgtcaa taacatagca cgtgatgctt aagagcgggt
     4261 aatactggtt tgcaagattg cactccgtta tcagtaagcc tacgctatgg aacacctcgc
     4321 gttcaggaga agaaaatgac aacaatgaat cctttccttg tgcaaagcac actgccgtat
     4381 ctggctcctc attttgatca aattgccaat catcactatc gcccggcatt cgatgaggga
     4441 atgcaacaaa agcgggcaga aattgctgcc atcgcgctta acccgcaaac gcctgatttc
     4501 aacaatacta ttctggcact ggaacaaagc ggagaattaa ttacccgcgt taccagcgtc
     4561 ttttttgcga tgactgcggc gcataccaat gatgaattac agcgtcttga tgaacagttt
     4621 tccgctgaac tggcggaact ggctaatgat atctatctga acggtgaatt attcgcgcgg
     4681 gtagatgctg tctggcagcg ccgtgaatcc ctggggcttg atagtgaatc catccgcctg
     4741 gtggaggtta ttcatcaacg ttttgtcctt gccggagcca aacttgcaca agctgataaa
     4801 gcaaaattaa aagtactgaa tacagaagct gcgaccctga ccagccagtt taaccagcgg
     4861 ttacttgcag caaataaatc cggcggtctg gttgtgaacg atatcgcgca gctggcagga
     4921 atgagtgagc aagagattgc gctggcggca gaggcggctc gcgagaaagg tctggataac
     4981 aaatggctga ttccgctgct gaataccacc caacaaccgg cgcttgccga aatgcgcgat
     5041 cgtgcgacgc gtgaaaaact gtttattgcg ggctggacgc gagcggaaaa aaatgatgcc
     5101 aatgataccc gcgctatcat tcaacgtctg gtagagattc gcgcacagca ggcgaaactg
     5161 cttggttttc ctcattatgc cgcatggaaa atcgccgatc agatggcaaa aacgccagaa
     5221 gcagcactca actttatgcg ggaaattgtt ccagcggcgc gtcaacgtgc gagcgatgaa
     5281 ttagcctcca tacaggcggt tatcgataag cagcagggcg ggtttagcgc gcagccgtgg
     5341 gactgggcat tttatgcgga acaggtacgg cgggagaaat ttgatcttga tgaggcgcag
     5401 ctcaagccat attttgaatt aaacacggtg ttaaatgaag gtgtattctg gaccgcgaat
     5461 cagctcttcg gtattaagtt tgtcgaacgt tttgatattc cggtctacca tcctgacgtt
     5521 cgtgtgtggg aaatttttga tcataatggc gtgggactgg cgttatttta cggtgatttc
     5581 ttcgcccgtg attcaaaaag cggcggtgca tggatgggca attttgttga gcaatcaacg
     5641 cttaatgaaa cgcatccggt aatttataac gtctgcaatt atcagaaacc cgctgccggt
     5701 gagcctgcgt tgttactctg ggatgatgta ataaccttat tccatgaatt tggtcatacg
     5761 ctgcacggcc tttttgcccg ccagcgttat gccacgcttt ccggcaccaa cacgccgcgt
     5821 gattttgtcg aatttccgtc gcaaatcaac gaacactggg caacgcatcc gcaggtattc
     5881 gctcgctacg cccggcatta tcagagcggg gcagcaatgc ctgacgaact gcaacagaaa
     5941 atgcgtaatg ccagcctgtt caacaaaggg tatgagatga gcgaactgct tagcgccgca
     6001 cttctcgata tgcgctggca ttgcctggaa gaaaacgaag caatgcagga tgtcgatgat
     6061 tttgaattgc gggcgctggt ggcggaaaat atggatcttc ctgctatacc gccacgctat
     6121 cgcagcagtt atttcgccca tatttttggt ggcggatatg ctgcaggtta ttacgcttat
     6181 ctgtggacgc aaatgttggc cgatgatggt taccagtggt ttgttgagca gggcggatta
     6241 acgcgtgaaa atgggcagcg ttttcgcgag gcgatccttt ccagaggtaa cagcgaagat
     6301 ctggaacgcc tgcatcgaca atggcgtggt aaggctcctc agattatgcc gatgctgcaa
     6361 catcgtggct tgaacatata aacaccacca atggctggcc tggagatatc gccaggccag
     6421 cctggttata gcagtaattg cagcagctgg gcgagggcaa acctcaccgc taaagctaat
     6481 accgtttcgc agtcgccagc aaaatgcata accgcagtgt agttctggcc tttaatatgc
     6541 cacgcgaacc agacggtacc cgctggcgta ccatcttcac cgccctccgg tccgccgtag
     6601 ccggtaatgg caatactgac atcagcatcc gcacgctcta tggcacctgt tgccatttct
     6661 gctgccactt tctcactcac cgcagaatat cgttcaagag actgctggct tacgctgagg
     6721 attttcatct ttgcctgatc ggtgaaagta acaaagcctg caccgtaaaa tttgggtgta
     6781 tcttcagctg cacacagggc gctagccaac ttaccgccgg tgcatgattc ggcggtagtt
     6841 aagcgtaatt gatccgcgat aagtctttga ctcaacgcgg atgtcaggtt ttcaatagtg
     6901 tcggtatctg cgagttgtac aattttatct ttatt
//
LOCUS       DAJTYM010000158         6849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTYM010000158 DAJTYM010000000
VERSION     DAJTYM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            294..446
                     /gene="hokE"
                     /locus_tag="OO575_003712"
     CDS             294..446
                     /gene="hokE"
                     /locus_tag="OO575_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6666510.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(568..1188)
                     /gene="entD"
                     /locus_tag="OO575_003713"
     CDS             complement(568..1188)
                     /gene="entD"
                     /locus_tag="OO575_003713"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6666511.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1363..3603)
                     /gene="fepA"
                     /locus_tag="OO575_003714"
     CDS             complement(1363..3603)
                     /gene="fepA"
                     /locus_tag="OO575_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6666512.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3846..5048
                     /gene="fes"
                     /locus_tag="OO575_003715"
     CDS             3846..5048
                     /gene="fes"
                     /locus_tag="OO575_003715"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6666513.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5051..5269
                     /gene="ybdZ"
                     /locus_tag="OO575_003716"
     CDS             5051..5269
                     /gene="ybdZ"
                     /locus_tag="OO575_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6666514.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5266..>6849
                     /locus_tag="OO575_003717"
     CDS             5266..>6849
                     /locus_tag="OO575_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCR6666515.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHA"
BASE COUNT         1519 a         1808 c         1832 g         1690 t
ORIGIN      
        1 cctgctttac actttcgccc gcggtcagtg atggctgcgg gcgatttcgt tttaagagca
       61 atgatgagtt ttgccgacag aagtccgaaa caggactcac tgtataaata aacagctatt
      121 ttgctaagga agggtaagat aacggcgggt gcctgaagct ttccggtttc aggttaactc
      181 tgaggtttgg aaagatgaag ccccaagaga tatttctatc aatcctgggg ctgccactcc
      241 gaacctcaac aactcggatg gtagtccctt cttcgcatgg aggcaatata aacatgctga
      301 cgaaatatgc ccttgtggca gtcatagtgc tgtgtttgac ggtgctggga tttacgcttc
      361 tggtcggaga ctcgctgtgt gagttcaccg taaaggaacg taatattgag tttaaggctg
      421 ttcttgctta cgaaccgaag aagtagccgt tgtgcgggga gtaatccccg catatccggt
      481 tgtcaggtca gagtggtaag gcacctgctt tacactttcg cccgcagtca gtgatggctg
      541 cgggcgaatc gtaccagatg ttgtcaatta atcgtgttgg cacagagtta tgactatttt
      601 ttcttttatc tgccagtgca cagcaaacat ctcattctca cgatgaatga tgacctgctg
      661 tttattccag ctaattatct gatagtccag aaaacctgca tcagtttgga tctcacttgc
      721 cttaaatgcg ctctctttgg cggaaaatgc aagtgtcagc gccagagaaa aggctaaacc
      781 gcagtctgcg agtcgctcgt gttctgctgg tgtaataatg ttgtctgtca attcggtcgc
      841 tgtttgcgca gaaaaaattt cttcgatatc aacgccaatc ggttgacggg aaaccacggc
      901 caatgccgta gccccgcagt ggctaatact gccgtatacc tccgcaggcc acaccggttg
      961 gcgtagctcg ccgattgcgg gcacacattt atagccatat tcccgcaagg cataaacagc
     1021 agcgatccgt ccggctaaat gctctgtttt acgtttacgt ccagcgtgtt gcagttgtgc
     1081 gtagtgcggc agccagagta aatcctgctc acaaaaattc gccggatcga actcaacaaa
     1141 atgcagcgta tgtccggcaa aggggaggga ggtatgcgta gttttcatat cgaccatatt
     1201 cgagactgat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtgatc
     1261 ctgcaatata ttgaattggc aagatctttg taggccggat aaggcgttca cgccgcatcc
     1321 ggcgtgaacg acgcgcactt tgtcaacaat ctgacgttag catcagaagt gagtattaat
     1381 gctcatatac cacgtacgtc ccggctcgtt ataggtatac gcgccggcac cggcgatata
     1441 gttggctcct gccagatcgc ccgtggtctg ggcattacct gcacgccaca aacgtttgtc
     1501 gaacaggttg tccacgccgc cggtcagact gacattcttc gtcacatccc aggtcgcgct
     1561 cagacccaca atgctgtaag gactgatttc tttggtttcc gggccaaccg ctggctgacc
     1621 tttatagttg tacttcttcg gctgctgctt gccgtaccag gtgaaggtcg tttgcatcga
     1681 caaatcttcc cgtgcctgcc agctcagcgt tgagttcaac gtgtactccg ggatgatcga
     1741 caaacggtcg cccgtggttt tgttttcact cttcagcata taagtgatgt tattggtcca
     1801 catcaccgtt tcgctaactg gtacgtttaa cgatccttcc agaccttcaa ccactgcttt
     1861 cggcacgtta tcccactgat agagatcagt gccgactgcg ttttgcccta cagccacata
     1921 gcccgcttca atcttattgc gataatcgtt acggaaccag gtgacgcccg ccagccagcc
     1981 gtcgcgtttg aactccagac caatctcttt gttgatgctg gtttctactt tcaggtcatc
     2041 gttaccttgc agatagcagc cgcccgcgct ggcatagcag ccctgacctt tactgtagag
     2101 aatgtagttc gggttagtct ggtataggct cggcgcttta taggcgcggg caatgcctat
     2161 tttcagcgtg aagtcatcgc ctaaaccttg cgatatgttc agcgccgggc tccagttatt
     2221 gccgacaata ctgtgatgat cgaaacgcag ccccggcgtt acgatggtgc tgtcagtcag
     2281 ctccatgttg ttttcggcaa acagcgagaa aatttctgct tttgaatacg ggctacggtc
     2341 ggtggcactc acgccatcaa tagcgccacc ggtattcgtt ccggtcagtg cttgggtgtt
     2401 ggaacttaag tccttcatcc gttgctgatt ccactccgta cccagcgtca gcgtctggtt
     2461 aacgaggaaa tcaatcggca ggttaacttc gctgtgcagc atcacgtcat cgagatcgat
     2521 atcgacgaaa tcctgtgtcg ctttttcgtt aaatttccct tcggtaccgc ccgccagacc
     2581 ttccggaata cgcgagttac gggtgtgttc atactgcacc cagttgctgg tggtcacgcc
     2641 gttatcccag ccaccgttcc aggtcagcgc gtagttctgg cgatacaggc ggttggtttc
     2701 atcgccatat ttcgagcgag tgtaagcgtc agagttggtg ttctgggtat cgcccgcata
     2761 caggttaccc tggcggctgt aacccgcttc cagttccagc gattgcaatg gtgcgaagtc
     2821 ccagcgcacc acgccgttaa tatctttatt gatcacccct tcgcgcccgg ctggtaacgt
     2881 cgtggcatac gttccggcac gcgcggactg atggccctgg ttgatatccc acgcgtcagc
     2941 ctgagttttg tcgaggttgc catacaagcg gaagctgaat tcgtcgccca gcggaccggt
     3001 caggctaaag ttagtgcgtt tggtggcacc ttcctcttta tgttctggcg cattgaaata
     3061 tgcgtcccag gagccgtgcc actcgccgct gccttttttg gtaatgatgt taaccacgcc
     3121 gcccgccgcg ccgttgccat aacgcgcagc tgccggacca cgcagaactt caatacgttc
     3181 aatcatttca ggtggcaccc aggaggtatc accacgggta tcgcgctcgc cacgccagcc
     3241 ctgacgcacc gagttacggc tgcttaccgg cttgccgtca atcaaaatca gcgtgttttc
     3301 cggacccata ccgcgaatat caatctgtcg gttattccca cgctgaccac tggtggagtt
     3361 accggtcagg ttaacgcctg gcatggtacg gatgatctcc gacacatcgc gggcaaccgg
     3421 gtttttgcgg atttcatctg cggtgatggt cgaaacgcca ggagcctgca agttctgctc
     3481 ggcggcggta acgacaatgg tatcgtcatg tgaaacaggt gtatcggtcg gctcttgtgc
     3541 ctgcgctacc ccataaatcc ccagattgac caacaaggcc agggaatgaa tcttcttgtt
     3601 cattgtttta ttcctgcatt tttgccacga attgcaactg tcgggcatgg tcgtcatcaa
     3661 cacgacgcat cccgttaccg cgaaaacctt tgatcctgaa agacacgcag tgcagttggt
     3721 taattaatgt ccgcgcttcc cacggcgcgc cattacgcta ttgcaaatgc aaatagttat
     3781 taataacatt atcaatatat ttctgcaatc aatgaaaaat tgcacagtaa acatggggtt
     3841 atggtgtgac ggcgttaaaa gtaggaagtg agagctggtg gcagtcgaaa catggcccgg
     3901 aatggcagcg tctgaatgac gaaatgtttg aggtcacttt ctggtggcgt gatccccaag
     3961 gttctgaaga atactcgacg ataaagcgcg tatgggtcta catcactggt gtgaccgatc
     4021 atcatcagaa cagccagccc cagtcgatgc agcgaattgc aggtactgat gtctggcagt
     4081 ggacgacaca actcaatgcc aactggcgcg gcagctactg ctttattccc accgaacgcg
     4141 atgacatttt ttctgcacca tcccccgatc gcctcgaatt acgtgaaggc tggcgaaaac
     4201 tattacccca ggcaatagcc gatccgctga atccacaaag ctggaaaggc gggctagggc
     4261 acgctgtttc tgcactcgaa atgccgcaag cgcctctgca accgggatgg gattgtccgc
     4321 aagcgccaga aataccagcc aaagaaatta tctggaaaag tgaacggttg aaaaattcac
     4381 gtcgtgtatg gatttttacc accggcgatg taacagcaga agaacgcccg ctggcagttt
     4441 tgctcgatgg cgaattttgg gcgcaaagta tgcccgtctg gcctgtgctg acttcgctga
     4501 cccatcgtca gcaacttcct cccgccgtgt atgtgttgat cgacgctatc gacaccacgc
     4561 accgcgccca cgaactgccg tgtaatgcgg atttctggct ggcagtacag caagagttat
     4621 tacccctggt gaaagttatt gcccctttta gcgatcgcgc cgatcgcacc gtggtcgccg
     4681 ggcagagttt tggtgggctg tccgcgctgt atgccggact gcactggcct gaacgctttg
     4741 gctgtgtatt aagccagtca ggatcgtact ggtggccgca tcggggcggg cagcaagagg
     4801 gcgtgttact tgaaaagcta aaagctggtg aagttagcgc cgaaggtctg cgcattgtgc
     4861 tggaagcggg tattcgcgag ccgatgatca tgcgggccaa tcaggcgctg tatgcgcaat
     4921 tacaccctat aaaagaatcc attttctggc gtcaggttga cggcggacat gatgcgcttt
     4981 gttggcgcgg tggcttgatg caggggttaa tcgacctctg gcaaccactt ttccatgaca
     5041 ggagttgaat atggcattca gtaatccctt cgatgatcca cagggagcgt tttacatttt
     5101 gcgcaatgcg caggggcaat tcagtctgtg gccgcaacaa tgcgccttac cggcaggctg
     5161 ggacattgtg tgtcagccgc agtcacaaga gtcctgccag cagtggcttg aagcccactg
     5221 gcgtactctg acaccggcga atttcaccca gttgcaggag gcacaatgag ccagcattta
     5281 cctttggtcg ccgcacagcc cggcatctgg atggcagaaa aactgtcgga tttaccctcc
     5341 gcctggagcg tggcgcatta cgttgagtta accggagagg ttgatgcgcc attactggcc
     5401 cgcgcggtgg ttgccggatt agcgcaagca gatactttgc ggatgcgttt tacggaagat
     5461 aacggcgaag tctggcaatg ggtcgatgat gcgctgatat tcgaactgcc agaaattatc
     5521 gacctgcgaa ccaatattga tccgcacggt actgcgcagg cattaatgct ggcggatttg
     5581 caacaagatt tgcgcgtcga tagcggtaaa ccactggtct ttcatcaact gatacaggtg
     5641 gcagataacc gctggtactg gtatcagcgt tatcaccatt tactggtcga tggcttcagt
     5701 tttccggcca ttacccgcca gatcgccaac atttactgcg cattgctgcg tggcgaacca
     5761 acgcctgctt cgccgtttac gcctttcgct gatgtagtgg aagagtacca gcaataccgc
     5821 gaaagcgaag cctggcagcg tgatgcggca ttctgggtgg aacagcgtcg ccaactgcca
     5881 ccgcccgcgt cactttctcc ggcaccttta gcggggcgca gcgcctcggc agatattttg
     5941 cgtctgaaaa tggaatttac cgacggggaa ttccgccagc tggctacgca actttcaggt
     6001 gtgcagcgta ccgatttagc ccttgcgctg acggctttgt ggctggggcg attgtgcaat
     6061 cgcatggact acgccgccgg atttatcttt atgcgtcgac tgggctcggc ggcgctgacg
     6121 gctaccggac ccgtgctcaa cgttttgccg ctgggtattc atattgcggc gcaagaaacg
     6181 ctgccggaac tggcaacccg actggcaacc cgactggcag cacaactgaa aaaaatgcgt
     6241 cgtcatcaac gttacgatgc cgaacaaatt gtccgtgaca gcgggcgagc ggcaggtgat
     6301 gaaccgctgt ttggtccggt actcaatatt aaggtatttg attaccaact ggatattcct
     6361 ggtgttcagg cgcaaaccca taccctggca accggtccgg ttaatgacct tgaactggcc
     6421 ctgttcccgg atgaacacgg tgatttgagt attgagatcc tcgccaataa acagcgttac
     6481 gatgagccaa cgttaatcca gcatgctgaa cgcctgaaaa tgctgattgc ccagttcgcc
     6541 gcggatccgg cgctgttgtg cggcgatgtc gatattatgc tgccaggtga gtatgcgcag
     6601 ctggcgcaga tcaacgccac tcaggttgag attccagaaa ccacgctgag cgcgctggtg
     6661 gcagaacaag cggcaaaaac accggatgct ccggcgctgg cagatgcgcg ttacctgttc
     6721 agctatcggg aaatgcacga gcaggtggtg gcgctggcga atctgctgcg tgagcgcggc
     6781 gttaaaccag gggacagcgt ggcggtggca ctaccgcgct cggtcttttt gaccctggca
     6841 ctccatgca
//
LOCUS       DAJTYM010000159         6726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTYM010000159 DAJTYM010000000
VERSION     DAJTYM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..413)
                     /locus_tag="OO575_003718"
     CDS             complement(<1..413)
                     /locus_tag="OO575_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HCR6666516.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQ"
     gene            complement(547..1368)
                     /gene="bglG"
                     /locus_tag="OO575_003719"
     CDS             complement(547..1368)
                     /gene="bglG"
                     /locus_tag="OO575_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6666517.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(1655..2380)
                     /gene="phoU"
                     /locus_tag="OO575_003720"
     CDS             complement(1655..2380)
                     /gene="phoU"
                     /locus_tag="OO575_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6666518.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(2395..3168)
                     /gene="pstB"
                     /locus_tag="OO575_003721"
     CDS             complement(2395..3168)
                     /gene="pstB"
                     /locus_tag="OO575_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6666519.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(3260..4150)
                     /gene="pstA"
                     /locus_tag="OO575_003722"
     CDS             complement(3260..4150)
                     /gene="pstA"
                     /locus_tag="OO575_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6666520.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(4150..5109)
                     /gene="pstC"
                     /locus_tag="OO575_003723"
     CDS             complement(4150..5109)
                     /gene="pstC"
                     /locus_tag="OO575_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6666521.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(5196..6236)
                     /gene="pstS"
                     /locus_tag="OO575_003724"
     CDS             complement(5196..6236)
                     /gene="pstS"
                     /locus_tag="OO575_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6666522.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(6484..>6726)
                     /locus_tag="OO575_003725"
     CDS             complement(6484..>6726)
                     /locus_tag="OO575_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6666523.1"
                     /translation="TDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGE
                     KSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         1688 a         1769 c         1569 g         1700 t
ORIGIN      
        1 ctttgttcgg tcgtccactg aaaagtgagc gccagagcca gcataccttt caggatcccg
       61 gttgccgcca tcagaccgat cagaggcgta aaaatacctg aaataacata aacaaagcgg
      121 tttagcagat tacctttctc atcattttcc ggtgcctgtt gcgctttttc gccaaggcct
      181 gccacgctgt taaccgccag gaagacatcg gccacatggt tacctatgac cacctgaaac
      241 tgaccaccgc tttccaccac cataataata ccgggggtct ttttcagtac ctcagcttgc
      301 gctttgcttt catcctttaa cttaaaacgt aatcgcgttg cgcaatgcat cagactcaca
      361 atgttatctg cgcccccgac tcctgcgact atttttctgg ctaactccgt cataacttgc
      421 cctctaccgc tttgcggcaa aactccaaaa aaaaacctga aaaaaacggc ctgacgtgaa
      481 tcaagcaatt tttttcaggt tttgcccgct tagtgcggta acaatccttt actcagtaat
      541 aatatttcag tgttctttgc gcacgcgctc tatatttatg gctaaaaaca taatctctgc
      601 gggtgaaatt ttacgttgat actgcaaacc aataaaaatg gcgatccgtt ccgcacattg
      661 ccatgcttgc gggtaatttt gttttactgc ttgttgtaat gattcatcac tatcgttaat
      721 tgaagcatgt tcaagaatac gccaggataa aaacttcaga tgtgtaacca gtcgctgata
      781 actcaagctt tcttcctggt aattaaggct gaactgaaat tttattaatt gcagcatttc
      841 gcgcattaac tgcgtgacac ctgcaacatc ctccatattt ccgctcattt gggcactgac
      901 cagatgcatg gcaataaagc ccacttcatc tttcggtaac tgcacgccca accgtttatc
      961 aataatggtt aacgcttctt cccctagctg gaactctttc gggtaaagcc gctggatatc
     1021 ccacagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt cagttagcga
     1081 gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga taagacgatc
     1141 acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc gcccgttcag
     1201 ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc tttcgccagc
     1261 gcgtttttga aagccaattc cgcgccccat gacgactttt tcccgctgtt gatcatcaat
     1321 aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag caaggacctt
     1381 ttttataaac aaaaaaaaac cgacttcacc agtattctct ggttatgtca ggttttgcct
     1441 gcgaatgcag taacaatcca gtcatttatt aatggttttt ataacgaaca tccaggttcg
     1501 gaaattaatt taattgcgtg cagagaaaat agcaatgcgc tattgataaa aatatgacca
     1561 tgctcgcagt tattaacttt gtgtaatttt aggaatttat aaagttatat ataacaaatc
     1621 ccaataatta agttattggg atttgtctgg tgaattattt gtcgctatct tttcccgcca
     1681 gcagtttatc cagctcatca ccgcctacat gacggaaatc ctgacccttc acgtagtaga
     1741 agataaactc acaaatattc tggcagcggt cgccgatacg ttcgattgaa cgcgcacaga
     1801 acagcgcggt aagtacgctc ggaatagtgc gcgaatcttc catcatgtag gtcatcagtt
     1861 gacgaacaat accttcgtat tcctgatcga cttttttatc ttcacgataa atacgtaccg
     1921 cttcgtcaat gtccatccgc gcgaacgcgt ccagcacgtc gtgcagcatc tggatggtat
     1981 gacggcccag cgactccaga cttaccagca acggctgatg ctgctgggag aatttctcca
     2041 gcgcagtacg gcagattttg tccgccacgt cgccaatacg ctccagctcg gcaatggttt
     2101 tactgatcac cataaccagt cgcaggtcgc tcgccgtcgg ctgacgtttg gcgataatgc
     2161 gcacgcaggc ttcatcgatc gccacttcca tcatgttgac gttcttgtcg ccttcgatca
     2221 cgcgcttcgc cagatcgctg tcctggttat gcatcgcggt gattgcatca gaaagctgct
     2281 gctccaccat gccgcccatg gtcatcacct gcgtgcggat actttccagt tcggcgttga
     2341 actggccgga aatatgttta ttaagattga gactgtccat aacgcactcc tgaatcaacc
     2401 gtaacgaccg gtgatgtagt cttctgtttg tttcttcgct ggcttggtga acagatcgtc
     2461 cgtgttgctg aactcaatca attcgcccag gtacataaat gccgtgtggt cggaacaacg
     2521 cgcagcctgc tgcatgttgt gggtgacgat caccacggtg taatcctgct tcagttcggt
     2581 gatcagctct tcaatacgcc cggtagagat ggggtcgagc gccgaacacg gttcgtcgag
     2641 cagcagcact tccgggcgaa tggcgatacc acgcgcaata cacagacgct gttgctgacc
     2701 accagagaga gagtaaccgc tctggtgcaa tttatctttg gtttcgttcc acaatgcggc
     2761 tttggtcaat gcccactgca cgcgctcgtc catgtcggca cgggagagct tctcaaacag
     2821 acgaacgcca aaggcgatgt tgtcgtagat ggacatcggg aacggcgtcg gtttctggaa
     2881 caccatgccc actttcgcac gcagcagggc gatatcctga gagttggtca ggatgttgtc
     2941 gccatcaagc agaatttcac cttccgcacg ctgctccggg tacagttcaa acattttgtt
     3001 gaaggtacgc agcagcgtcg atttaccgca gccggacggc ccgataaacg ccgtaacctg
     3061 gtttttggcg atatccaggt tgatgttttt cagggcatgg aatttgccgt agtagaagtt
     3121 caaattacga acctgaattt tactcggggc agtttcaacc atactcattg caatcttttc
     3181 ctcatcgtac ctgatgcttt tcgctaatca ggcttaccgt agggccgggt aaggcgttca
     3241 cgccgcatcc ggaaaagtgt taaccgtgtt tattcttcgc aaaaacaacg cgcgccagaa
     3301 tgttcagcag caggacgcac agggtaatga tcaatacccc ggcccaggcc agttgttgcc
     3361 attccgcaaa cgggctcatc gcaaacttaa agatcgtcac cggcaggttg gcgatcggct
     3421 gcatcatgtc cgtgctccag aactggttgg agagcgcggt aaacagcagc ggcgcggttt
     3481 caccggcaat acgggcaatc gccagcagga taccggtcat aatcccggac accgacgctt
     3541 tcagcgtaat cgcggagatc atcttccact tcggtgtacc cagcgcataa gccgcttcac
     3601 gcaggctgtc cggcaccagt ttcagcatgt tctcagtggt gcggataacg atcggcacct
     3661 gcaacagcgc cagcgcaatt acgcccgccc agccggagaa gtgctccatc tgtgccacca
     3721 cgatggtgta gacaaacaga ccaaccacaa tcgacggcgc ggagagcaga atgtcgttaa
     3781 tgaagcgaat cacttctgcc agccaggatt tacgaccata ttccgccaga taaatccccg
     3841 ccataatgcc cagcggcgta ccgaataccg tggcccacaa aattaacagc ccgctacccg
     3901 ccagggcgtt cgccagacca ccaccttccg tattgggcgg cggcgtcatt tcagtgaaca
     3961 gcgccagcga cataccgtcg ataccgcgag tgatggtgga cattaaaatc cagatcagcc
     4021 agaacaggcc gaaggccatc gtcgccatcg agagcgtcag cgcaatacgg tttttgaggc
     4081 ggcgacgcgc ctgcattttg cggcgagatt cagccagcgc cgcagtggtt tgcatttcaa
     4141 ccatagccat tagcgtgccc cctcattctt agccaggcgc ataatcataa acttcgatgc
     4201 ggcgaggacg atgaaggtaa tcacaaacag gatcaggccc agttccatca gtgcggcaac
     4261 gtgcagaccg gattccgctt ccgcaaattc gttcgccagc gcagaggtga tactattgcc
     4321 cggcatgtac agcgaggcgc tgtcgagctg gtaggtgtta ccgatgataa aggtcaccgc
     4381 catggtttca ccgagcgcgc ggcccagccc cagcatgatg ccgccgataa caccattttt
     4441 ggtgaacgga agaacgatac gccagataac ttcccaggtg gtgcagccaa taccgtaggc
     4501 cgactctttc atcatcaccg gggtttgttc gaacacatca cgcattaccg ccgcaatgta
     4561 cggaataatc atgatggcga ggatcacgcc tgccgcgagg ataccgatac caaatgcggg
     4621 gccagagaac agcgcgccaa caatcgggat attcgacatg atattgccga ccggctcctg
     4681 aaagtaaacg gcgaacagcg gcgcaaagat aaacaggccc cacatgccgt aaacgatact
     4741 tggaatggct gccagcagct caatggcgat acccagcggg cgtttcagcc agccaggcgc
     4801 aagctcagtc aggaacaggg cgataccgaa actcaccggg acggcgatca gcagcgcgat
     4861 aaacgaagtc accaacgtac cgtagatcgg caccagcgcc ccgtagatat cgttcggtgc
     4921 atcccactct ttggtccata ggaaagccag accaaatttc tgaatgctcg gccaggagga
     4981 gatgatcaga gagacaataa tgccacccaa catcaatagc acaatcagcg ccgccagttt
     5041 taccagcacg ctgaaaatta tgtcgccctt tttacccggt gggttaaaag cgggcttggt
     5101 tgcagccata agttactctt cagttaaacg cgtttacaaa gtcattttgt aatgccggat
     5161 gcggcgtaaa acaccgtacc cggcctggag ttttattagt acagcggctt accgctactg
     5221 tctttaatat tggtcttcca cgcagcgcga acctgttcaa ctacactatc cggcaggctg
     5281 gcgtaatcca ggtcgttcgc ctgtttagcc ccggttttgt acgcccagtc gaagaatttc
     5341 agcacctctg tgccttgttc tggtttcttc tgatctttgt ggatcagaat gaacgtggta
     5401 gaggtaatag gccatgcatc ttcgcctttc tggttggtca gatcctgagc gaaggttttg
     5461 ctccagtctg caccttttgc tgcattagcg aagttttctt cggtcggact aaccggttta
     5521 ccatcagcgg agatcagttt ggtgtacgcc aggttgttct gcttcgcgta agcatattca
     5581 acataaccaa ttgcacccgg cagacgctga acgaacgcgg cgataccgtc gttaccttta
     5641 ccgcccagac cgatcggcca ttttacggta gagccagtac caacgttgtt tttccactct
     5701 tcgttcactt tcgccaggta gctggtgaag acgaaggaag tcccggagcc atctgcgcgg
     5761 cgtactacag caatgttttg tgaaggcagt ttcagacccg gattcagttt ggcgatggct
     5821 tcatcatccc acttcttgat tttgcccagg tagatgtcgc cgagggtttt accatccagc
     5881 accagttcgc cagacttcag ccctggaatg ttaaccgcca gcaccacgcc gccaatcacg
     5941 gtcgggaact ggaacagacc ttcctgagcc agtttttcgt cagacagcgg cgcgtcagag
     6001 gcaccaaaat caacggtatt agcgataatc tgttttacgc caccggaaga accgataccc
     6061 tggtagttaa ctttattacc ggtttctttc tggtaagtgt cagcccattt ggcatacacc
     6121 ggcgcaggga aggttgcacc tgcacctgtc aggcttgctt ctgcaaacac agagaaagca
     6181 ctcatcgata aggtcgcggc gacaacagtt gcgacggtgg tacgcataac tttcataatg
     6241 tctcctggga ggattcataa agcattgttt gttggctacg agaagcaaaa taggacaaac
     6301 aggtgacagt tatatataag gaatatgaca tttttatgac agaaaggaat taccaaataa
     6361 cttactgatt ttaaatgtga agatgaaaat tccacatgcc atctttttgt catgatgcat
     6421 ttaaaagtga taaagaaatg ggtgagatga caacgatgtg atgaacaatc gttgtcagac
     6481 ctattagcgc acaatctcaa cagttatcgt cgctgttgca gtaaattgcc ccggggctgg
     6541 gcgagctcca gtaaggctcg caggagcagc tgaaaaagtt acactacctg atttttcacc
     6601 atacactgtt gatttatcca tgtctgttgg caactcacca ccgttcactt tgatttcctt
     6661 attatttttg tcgtaaattt ttatccccac gttagagttt gaagttacga ccattgaatt
     6721 gtccgt
//
LOCUS       DAJTYM010000160         6579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTYM010000160 DAJTYM010000000
VERSION     DAJTYM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..109
                     /locus_tag="OO575_003726"
                     /pseudo
     CDS             1..109
                     /locus_tag="OO575_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            634..1812
                     /gene="ltrA"
                     /locus_tag="OO575_003727"
     CDS             634..1812
                     /gene="ltrA"
                     /locus_tag="OO575_003727"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6666524.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1895..2074
                     /locus_tag="OO575_003728"
                     /pseudo
     CDS             1895..2074
                     /locus_tag="OO575_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2104..2586)
                     /locus_tag="OO575_003729"
                     /pseudo
     CDS             complement(2104..2586)
                     /locus_tag="OO575_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2842..3822)
                     /locus_tag="OO575_003730"
     CDS             complement(2842..3822)
                     /locus_tag="OO575_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6666525.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3822..5021)
                     /locus_tag="OO575_003731"
     CDS             complement(3822..5021)
                     /locus_tag="OO575_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6666526.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5343..5681)
                     /locus_tag="OO575_003732"
                     /pseudo
     CDS             complement(5343..5681)
                     /locus_tag="OO575_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5748..6530)
                     /gene="istB"
                     /locus_tag="OO575_003733"
     CDS             complement(5748..6530)
                     /gene="istB"
                     /locus_tag="OO575_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR6666527.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1757 a         1526 c         1576 g         1720 t
ORIGIN      
        1 ccatgccgat gatatccccg ttccggtcca ggagccgggc agcggtaaaa caaggactgc
       61 ccggctgtgg gtctacgttc gtgatgaccg taacgccggt tcggaaattg cagcataagt
      121 tatccatgct ctgaggtggg tagaggtggg ccctccggtc agggtttaca ggagcacaga
      181 ccaaacgacc cggtgctgct tcattctaag gaatgaggtg gtgagcgact cgggaaaagg
      241 cagagatcta ctgtcacgtg ggttcgggaa gctgaacgaa agtaaactgt ccgatgaagc
      301 atcgaaatct tagatgatgc caaaatgggg ccgtacagcc gtcccctgat aagcgcagaa
      361 gacacctgct tactgactgc gcggcatccg gtgtgaaggc agcaggatcc cgggccagac
      421 catctcaggg aacgtgagaa cctgtttccg gcacgtatcg ggaaacaggg gcggacccat
      481 tcgtagtagt gatgaaagat ctgtaatggg tctggagcga agggatgggg atcacattca
      541 ttgcagaaga cacaacccaa aaggggatga cgtaactgat gcaacgaaaa tcatttgaaa
      601 taccaaaagc actggtctgg gcatcatatc tggatgtgcg tcgcaacaag ggagcccccc
      661 ggatgcgacg ggcagacttt gaaaatgttc gaccagcaac gtgatggcaa tctatacaag
      721 atctggaacc ggctatgttc gggaacatgg tttccgccac cagtgctgga aaaacggatc
      781 ccgaagtcga atggcaagga acgcattctg gggatcccaa cagtatccga tcgaattgct
      841 cagggagcga taaaactttt catggaagaa aagcttgacc cgattttcca tgctgattca
      901 tatggttacc gaccaggcaa atcagctcat gacgctctga aacaatgtgc catccggtgc
      961 tggcgttata gctggatact ggaagtggac atcagtgcgt ttttcgacca tgtgagacat
     1021 gatctggtgc tcaaagctct ggaacaccac gggatgccta aatgggtcat cctgtactgt
     1081 cgacgctgga tggaagcgcc aatgcagagt tgtgaaaatg gagaattaat aacccgaaca
     1141 cgaggcactc cgcagggcgg ggtcattagt ccgctacttg ctaacttgtt ccttcactat
     1201 gcatttgatt tgtggatgga aagagaatat cggggggtac cgtttgagag gtacgctgac
     1261 gatattgtag tacattgttc acgaatgagt gatgcaacaa ggctaaagaa caggttatcg
     1321 gagcgcttct cggaagtcgg gctggtcctg aatgcaggga aaacgaatat cgcctacatt
     1381 gacacgttta aaaggagaaa cgtcgcaacg agtttcacct ttctcggata tgacttcaaa
     1441 gtgcgtacgc tgaagaattt caacggcgaa cggtaccgaa aatgcatgcc gggtgcgtca
     1501 aatgcagcaa tgcgcaaaat aacagaaaca atcaagaagt ggcgtataca tcgctcaaca
     1561 gctgagagtt tgctggattt tgcgaggcgc tacaatgcga tagtgagagg ctggatcgag
     1621 tactacggaa agttctggtc cagaaatttc aactatcgac tgtggagtgc aatgcagtca
     1681 cgtctgctca agtggatgca gtctaaatac agactttcga accggaaggc tcagcgaaag
     1741 ctgacgctgg taaggaagga gtatccgaag ctatttgttc actggtattt actacgtgca
     1801 tcaaatgagt gatcaagagc cgtatgacgc gagagtgtca cgtacggttc cgtgagcagc
     1861 cggggagtga aattctctcc ggctgactcg actcgcgggt tccgacagcg atgacgaaca
     1921 tgcggcggtg ttgtactcgc tgatcggcac atgccgtctg aacaatgtgg aaccagaaaa
     1981 atggctgcgt tacgtcattg agcatatcca ggactggccg gcaaaccggg tacgtgatct
     2041 gttgccctgg aaagttgatc tgagctctca gtaaatatca atacggttct gacgagccgc
     2101 ttacttttgg cgtaattctg ctgcgccttt actgaacaca atgccgtatt cagcaagaag
     2161 tccacgaatg tgatttatca gttgtgtcct ttccattgaa accaaccgac gcatattcaa
     2221 aaaagtctgg acgtcctgtt gctccagagt ttttattggt acgggtcgta tagttccatg
     2281 ttggcaggcc tcagctattg cctgtgcgtc gttataatca tttttctggc accgctgata
     2341 agctttaaca tgttgtgccg gaagcaatat tacatcatgg tcgagtttcc tgatttcccg
     2401 ggcccagtaa tgagatgccc cgccagcctc catcgcaaca gtcattttag gctgttgcac
     2461 cagccagtta agcagttgat tgcgagaaag tttggtcttt cctgcgggtt tgccatgagc
     2521 attgatactg aaaagataaa agttcgtttt agccagatca atgccggtga ttttctgttc
     2581 agacatggta tcccgcttac ttttgttgtt aaagtttatc catcatggcg caattgaagg
     2641 ctgccgtgaa agagggggaa gactatctca tcggttactg ttaaacggct aagagaaact
     2701 atctttttca agcaaagaag atacggtgaa acaggcgatt tcaccgtgaa atactgttca
     2761 aaaaaaacgc taataaaagc aaaatttctt ttaaaacagc caattaaaaa ctggcgagtt
     2821 gctggcgaaa aacttgaatt ttcacagcga attcagatta tcttttagta caagagcaat
     2881 catacgatct aacttatcct gaacttccaa cggtaatctg ccgaattcat atgagaacgt
     2941 acgtcccttt actcgttttc gagcaaattt atctttacta tcaaatttcc ataacaaagt
     3001 tacaaccgct ttatccttta ctcctgaatc aagcaatgaa gacatttcct tggttataag
     3061 ccgcataata ctgtttttaa cctcgtcttc agaaagattg tcatcagcag taataagctc
     3121 aatactcggc gagatattct tcacaacttc atcaatagat atatcaccca gcctcagtcg
     3181 ttcacttaat tcagccagtt gcctgtaatc ggcgtatgtc agctcagact gaacaggaaa
     3241 aagagacaca aaatctttag gtacggacgc tgcttggagg gcacgagtca ccttagctgc
     3301 cgacaatccc tctctttccg caatttgctt ctggttcata ccggcctctt tgagacgtac
     3361 taaacgcaac ccaatttcac gaatattatg ttcaagtgac gtctgcaaat ctttcgccag
     3421 atgctgagct tcagaaacag taagctcatc atccgttaca agtacacgta atccaacctt
     3481 gcatagcaaa gctgctgccc gacgacggga accatccaaa atctcaataa gatcgccagt
     3541 ccgaatccca atacatggat agaattgttg taaacgtata gttctggtga tgtcttttag
     3601 tgagtccggc gtcagggctg tctgttcgcg gccattcacc tcttcaacca caaaagtttg
     3661 agtatccact ttgtcatgat caaccttgat ctcagtaaat tttgcctttc ggccagtatt
     3721 gagggtaaat acatgaactg gagccgatat ttcttcagta ttattcagga tatttgtatt
     3781 cagcgtacgg ccaatagtcg gtcggtgctt tctgttttcc atcagtagtt ggcccttata
     3841 aattcaatac gatcaaaaac tgctttagcg aaatcttctg ctgccatacg tgcatttttc
     3901 aaagcttcac cactgccaac ataagtaaca ggattcgcag atatcacggt atcaaacgac
     3961 tcgccgcttc tctcaaaacc atcaagacgt ggcattgaaa catcaagcat gtcaccaccg
     4021 aaaatctctt ttgtcaggct atggcagtat ttatgatctg acttattcgc cagtttcgac
     4081 atgaagccaa tatttgcctg caaacggcat gaacatccat cctgttcaat aatctgtaca
     4141 agttcaggta aacgagccaa atatttaagc gtggaatgga aatcaacttg tgctggcggc
     4201 accggtgtaa acatgatatc tgcggcagca atggcatttt tcaaaaatgc gtccagatgc
     4261 gggcctgtat caataaggat gaagtcgaaa tcatgcttca gcttatcaat aatattctca
     4321 cgcaatatcg cgtgtttgtt ttgtcctaat agatgctcct cgcacagagt atcccagttt
     4381 gatgcaataa aggcgtcgtc aatagaagcc ggcataacat aaacgccagg gataactgac
     4441 ggaacgataa aatcttccag taattcctca cgactcacgt tctgcaacat agcctgcggt
     4501 gcggttgtat caaccagccc caccgcatgc aagtagttca gaaacatagt ggctgatgac
     4561 tggggatcta aatcaaggag cagaatacgt aaatcttctg acagcaggtg aggatgagca
     4621 cgcagggcgt gagctacgga aacactactc acggttttag acacaccgcc ttttagacta
     4681 ccgatgaata tggaatatgc ctcactataa cgatcgcgat atttaggaat gccacgatgt
     4741 gcatagatat cgataatgtt ctggatggtt aatgcatatt ttgtcgcggt tcctgctggg
     4801 cgtttttcaa actgatagcc ctgagcctcc atctcaccga ctgcaccatc cacgcttgcg
     4861 cgcgttaatt ttggaagctt agctacagca gactttgaat agacttgata aaaaacatca
     4921 gcctgaaact cctgcttttg ctcctgaatc tgttttgtta gggcgagcaa cattttatct
     4981 gcacgctgcg caaccttact taactgctca aaactagtca ttccccctcc gtgctatgta
     5041 ggattttaac ttattacaaa aaaaactgca ttagcagggt aatgcatgca agtacatatt
     5101 gtacatgctt atattaaaaa tgcgcgcatt gctgatttat taatcatcgg aatgaatatt
     5161 actgtaagat agtatgcctt tccgggagaa agacgctgat ttcccccgaa taatccagtt
     5221 caccaccaca ctccggacaa cagctttctt caggctccag atggtgtatt tcgcgggaaa
     5281 catgttccgg taacggacgg cgatgacgtg actgtcgcaa caggcggggg tagtgcttcc
     5341 aatcagtaga acatgtgctt ttcaatgaag gagccgatga ctttttcgtg gatttccacg
     5401 gaacgcgaga agcagattgt tttacgtgcc agccgcttga tgcgggtgcg gagggtccgg
     5461 ttgttacgtg caatacgttg cgtgaagatt ttgccggtca ggtgtttctc cttcggcact
     5521 tctctggcgt agctgcccca gtcatcgctg gtcaccatgc cgatacagaa cggagtcaac
     5581 agggctaaca gttcccgaca ggtctcatca ttccgtggcc cgaaagtgta agccaacaca
     5641 ccgccagttt tagtgttgta cgcataccag agccagtgtt gtgtcaacga cggatgaaaa
     5701 gtgatccact tatatctcca ccaacggccc aatattgatc caccgtttta ctcaggatta
     5761 gcttctgcta taaccccggc ctttcgtttc tgtctgagtc gatagctttc tcctttgatt
     5821 tgaacgacat gtgagtggtg taagatacgg cccagcatcg ctgaggtcag tgctgcatca
     5881 ccggcgaacg tttgatccca ctgcccgaac ggtagattgg atgtcaggat cattgcgctc
     5941 ttttcgtaac gtttagcgat gacctggaag aacagttttg cttcttcctg actgaacggc
     6001 agatagccta tttcatcaat gatgagcagg cggggggcca ttactccacg ctgaagcgtc
     6061 gttttataac ggccctgacg ttgtgccgta gataactgaa gtaacagatc tgctgctgtt
     6121 gtgaagcgaa ctttgatacc tacacggact gcttcatagc ccatcgctat tgccagatgg
     6181 gttttcccca cacctgatgg ccccagtaat acgatatttt cattacgttc tatgaagctg
     6241 agtgagcgta acgactggag ttgcttctgc ggtgctccgg tggcgaatgt gaagtcatac
     6301 tcttcgaacg ttttcaccgc cgggaaggct gccattcggg tatacatcgc ctgtttacgt
     6361 tgatgacgtg ccagtttttc ttcatgaagc agatgctcca ggaagtccat ataactccat
     6421 tcctggtcta ctgcctgttg tgacagcgca ggcgctgcgc ttataaggct ttccagttgc
     6481 aactgcccgg cgagcgccat cagtcgttga tgttgcagtt ccatcatcac gccactcctc
     6541 tgcagaatga gtcgtagatg gagagtggat gatgctggg
//
LOCUS       DAJTYM010000161         6564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTYM010000161 DAJTYM010000000
VERSION     DAJTYM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            102..1526
                     /gene="sbcB"
                     /locus_tag="OO575_003734"
     CDS             102..1526
                     /gene="sbcB"
                     /locus_tag="OO575_003734"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6666528.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1569..1796)
                     /locus_tag="OO575_003735"
     CDS             complement(1569..1796)
                     /locus_tag="OO575_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6666529.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1810..2868)
                     /gene="yeeE"
                     /locus_tag="OO575_003736"
     CDS             complement(1810..2868)
                     /gene="yeeE"
                     /locus_tag="OO575_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6666530.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3047..4405)
                     /gene="plaP"
                     /locus_tag="OO575_003737"
     CDS             complement(3047..4405)
                     /gene="plaP"
                     /locus_tag="OO575_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6666531.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4395..4457)
                     /gene="yoeI"
                     /locus_tag="OO575_003738"
     CDS             complement(4395..4457)
                     /gene="yoeI"
                     /locus_tag="OO575_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6666532.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4618..5601)
                     /locus_tag="OO575_003739"
     CDS             complement(4618..5601)
                     /locus_tag="OO575_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6666533.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5647..6471)
                     /locus_tag="OO575_003740"
     CDS             complement(5647..6471)
                     /locus_tag="OO575_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6666534.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
BASE COUNT         1782 a         1725 c         1546 g         1511 t
ORIGIN      
        1 aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc ttttcgagtt
       61 ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac acagataaac
      121 aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg ttagatcgcc
      181 ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc gaacccgaag
      241 tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta ttaattaccg
      301 gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt gccgcccgta
      361 ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat gtgcgtttcg
      421 acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac gcctggagct
      481 ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt tatgccctgc
      541 gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt cgccttgagc
      601 atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg gctgatgtgt
      661 acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg tttgattatc
      721 tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg cagatgaaac
      781 ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc tgggtggcac
      841 cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg gcaggagaca
      901 tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat accgcaaaag
      961 ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat aaatgtccgg
     1021 tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga attaatcgtc
     1081 agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg
     1141 tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat gcacagcttt
     1201 ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg gaaaccgagc
     1261 cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa aagttgttgt
     1321 tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag caacgctggc
     1381 tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct gatgaattgc
     1441 agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta aaagcacttt
     1501 ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg gcggaggaca
     1561 atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat ctgctgataa
     1621 tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg
     1681 caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc tgcttttgct
     1741 tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat cgccatcttt
     1801 acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata
     1861 cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa tccagccctg
     1921 ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac ctgcaatact
     1981 ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt gtgcatcagc
     2041 tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca cgaagatccc
     2101 taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca gaaattgaag
     2161 aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg cttcactcag
     2221 gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg gatgccagcg
     2281 tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag
     2341 ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca aaacggtaac
     2401 caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag tagtgtagtg
     2461 ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac tcatcaccat
     2521 ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat accaggtccc
     2581 ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac ctataacagt
     2641 accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa
     2701 aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc gattattttt
     2761 cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct gcatgacaaa
     2821 tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa attaattaac
     2881 cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca ataaccaaat
     2941 tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa taaaaaaacc
     3001 ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat gctacgtctt
     3061 cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa atcagaccga
     3121 tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac
     3181 caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct
     3241 tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca aaggtgaacg
     3301 ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag ttgattgcca
     3361 gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat ttcgggtgta
     3421 cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac atcagacgcg
     3481 ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg
     3541 cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg
     3601 gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga
     3661 agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg cgctctgcat
     3721 ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga
     3781 ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac ggacgcgtgc
     3841 tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca
     3901 ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct acggatttca
     3961 gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg
     4021 gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg
     4081 cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg
     4141 atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc agcttcccgt
     4201 agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac
     4261 catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc tgcatatagg
     4321 ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg
     4381 tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac cgtcgcgtaa
     4441 gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga tttttagtaa
     4501 atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga agcaaaaaat
     4561 aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc attgtgccta
     4621 aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc tttgaatggc
     4681 tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg ctaaaatttc
     4741 atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa tccagacagg
     4801 ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat cgagaaaacg
     4861 ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca gctccagttt
     4921 ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg aggccgtaga
     4981 agcgcctgcc tgcgcagtat cccccaccac aatcgcccga tatcgtttaa tgatacgacg
     5041 gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa agacttgttc
     5101 aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat gcatcgcgcc
     5161 aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga gtacgccatt
     5221 gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga gaggcgcaag
     5281 cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc agccttcatg
     5341 cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc gccctttctc
     5401 taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat caagcaattg
     5461 aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta
     5521 gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga ggatcatcaa
     5581 cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc ctgctggcgt
     5641 gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa atcccagttc
     5701 attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac tatttctgaa
     5761 ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat tacgcgcagg
     5821 gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta acaacagagt
     5881 gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac catcaggggc
     5941 ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc ccgcaagacg
     6001 cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt cttctaatac
     6061 tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc cttgcgcgtc
     6121 gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg ccagcgcgct
     6181 atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc tacgacgtgc
     6241 cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt cagaatcgca
     6301 aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc gggccgcttc
     6361 gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg aaagtgacat
     6421 cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca tccgttatct
     6481 cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca attcattcta
     6541 tgaatgaatc tgttcaataa tgat
//
LOCUS       DAJTYM010000162         6540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTYM010000162 DAJTYM010000000
VERSION     DAJTYM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            7..1065
                     /locus_tag="OO575_003741"
     CDS             7..1065
                     /locus_tag="OO575_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6666535.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1108..1611
                     /gene="yeaK"
                     /locus_tag="OO575_003742"
     CDS             1108..1611
                     /gene="yeaK"
                     /locus_tag="OO575_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6666536.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1612..1716)
                     /locus_tag="OO575_003743"
     CDS             complement(1612..1716)
                     /locus_tag="OO575_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666537.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1886..2332
                     /locus_tag="OO575_003744"
     CDS             1886..2332
                     /locus_tag="OO575_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6666538.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2289..3107)
                     /gene="nimR"
                     /locus_tag="OO575_003745"
                     /pseudo
     CDS             complement(2289..3107)
                     /gene="nimR"
                     /locus_tag="OO575_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3204..4384
                     /gene="nimT"
                     /locus_tag="OO575_003746"
                     /pseudo
     CDS             3204..4384
                     /gene="nimT"
                     /locus_tag="OO575_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4439..4786
                     /locus_tag="OO575_003747"
     CDS             4439..4786
                     /locus_tag="OO575_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6666539.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4808..5062)
                     /gene="yoaF"
                     /locus_tag="OO575_003748"
     CDS             complement(4808..5062)
                     /gene="yoaF"
                     /locus_tag="OO575_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6666540.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5245..6270
                     /gene="dgcP"
                     /locus_tag="OO575_003749"
     CDS             5245..6270
                     /gene="dgcP"
                     /locus_tag="OO575_003749"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6666541.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6303..6401
                     /locus_tag="OO575_003750"
     CDS             6303..6401
                     /locus_tag="OO575_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6666542.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6404..6478
                     /gene="yoaJ"
                     /locus_tag="OO575_003751"
     CDS             6404..6478
                     /gene="yoaJ"
                     /locus_tag="OO575_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6666543.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1580 a         1585 c         1606 g         1769 t
ORIGIN      
        1 tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc tgccaataat
       61 acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac actacaaacc
      121 gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc ttttgatcag
      181 gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat ggccgaagag
      241 aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga gccggttaat
      301 gttgaatact ttgcgatgaa taactggtcc ttcctgcagt caggaagtat tggcatcgat
      361 cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat ttaccaggat
      421 cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc agggcagcta
      481 aaagggattg tgctgctgga tattaacaaa aacaatttgc ggaatatctt ttacactcat
      541 gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgataccga ttcggggcgc
      601 gacattatca tcaaccagag cgaagataat ctgttccagt atgtcagtta cgtccatgac
      661 ttacctggcg gcattcgtgt ctcgttatcc attgatattc tttactttat cacgtcttcg
      721 aggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat ggtccggatg
      781 cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat gactggactg
      841 tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact ggtgcaatcc
      901 ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagcaaat aaatgacacc
      961 ctcggtcatc aggaggggga tttagcgatt acgttacatg atgcgtataa agcttccgat
     1021 gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc ttctgtggtt
     1081 gtttgcttgt aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag cgtgacgcat
     1141 cagcagttaa tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt tacccatgaa
     1201 gcggtaggta aatgtgaagc ggtgtcagaa attcgtggta ccgctctagg ccagggtgct
     1261 aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct ggcgatcctc
     1321 gcagccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg attacgcgct
     1381 tcgttggcca gcccggcgga agttgatgaa ctcacaggct gtgtcttcgg cgcgatcccc
     1441 cctttcagct tccatccaaa actcaaactg gttgccgacc ctctactctt cgaacgattt
     1501 gatgaaatcg ccttcaatgc agggatgctg gataaatccg ttattttgaa aaccgcggat
     1561 tatttgcgca ttgcgcaacc agaactggtc aatttccgcc gcactgcgta gctagcctat
     1621 ccgttcaata agcagaacgg acaaaaccag cacaaccgca atagcaaaaa acgatgatgt
     1681 gataatcagt gtctcgacga acatttgatc gttcatagca tgcccccggg agatagctgt
     1741 tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc tgatttttta
     1801 tgcaaaaaac gtaaagattt tctacttcct tcctgcagca agcataaagt aagcaggctt
     1861 attatttttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt
     1921 agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc tggtgttgat
     1981 cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac aggggctaag
     2041 tatcgggata atcattctga cgattggcgt tatggccccc atcgccagcg ggacgctacc
     2101 gccctcaacc ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtagg
     2161 cgttattgtc tcctggctgg gtgggcgcgg cgtgacgtta atgggcagcc agccgcaact
     2221 ggttgctggg ctgctggtgg gcactgtttt aggcgtagcg ctgtttcgcg gcgtaccggt
     2281 tggcccgctt attgccgcgg gtctggtttc gctgattgtg gggaaacagt agttaatccc
     2341 gcaatatatc ggcctggcgt ttgccccaag cctttcttaa acatagtaat aaaggccgtt
     2401 gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt cactaatccc
     2461 tgaagcgcca taatcagctg taactgctga cgccactggc gaaaactcaa ccccgtctct
     2521 ttcactatca ggcgggcaag attgcgttca ctcatcgcaa aaaagccagc ccattgcccc
     2581 aatgcccccc actcgacggg ccctttcgcc atcatctcca ccatcgtgcg gattttagga
     2641 tgagaagaaa cgggcaggtg cagctgttgt tgtggctgct gagggagttc atcaaacagg
     2701 acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct gtttgccagg
     2761 gttaaaatca attcccggca taagggcgat attttcaatg tgcaacatgg tcggcattgt
     2821 cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca cttgattgct
     2881 atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc acaaagcatt
     2941 ttccaccgta caggtaattg caccatgtaa tgccagtatc agctgtcctt tgcggtgctg
     3001 atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga ccgcggcttc
     3061 atggtgtcgg tgggttcata gccattgaga ttcaacctgt gcatcatttt gtccgaactt
     3121 agcgataatt tgtcatttta gcttgattca acataacaat aaaaacggta aggtacagcc
     3181 tcgtttgtaa caatgagaag catatgacct gttcaacttc attaagcggc aaaaacagga
     3241 ttgtccttat cgctggcatt ctgatgattg ccacaacatt acgcgtcacc tttaccggcg
     3301 cagcgccgtt actggatacg attcgttccg cttactcgct gacgacagcg caaaccggct
     3361 tattaaccac cctgccatta ttggcctttg cgctaatctc acctctggct gccccggtgg
     3421 cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt acttttgatc tgtgcaggta
     3481 tcgcaattcg ttctctccct tcgccttact tattatttgg cggtacagcg gtcattggcg
     3541 gtgggattgc attaggcaat gtcttactgc caggattaat taaacgcgat ttccctcatt
     3601 ccgtcgccag acttaccggc gcatattccc tgacaatggg agctgcagcg gcactgggat
     3661 cggctatggt cgtgccgctg gctttgaacg gttttggctg gcaaggcgcg ttgttcatgc
     3721 tgatgtgttt tcctctgctg gctctttttt tatggctgcc acagtggcga agtcagcaac
     3781 atgcaaattt gagtacctca cgcgccttac atactcgggg tatctggcgt tcgccacttg
     3841 cctggcaggt aacattgttt cttgggatca actcactggt ctattacgtg attattggct
     3901 ggcttccggc gatcctcatc agtcacggct atagcgaagc acaggcgggt tcactgcatg
     3961 gtttgctgca actagccaca gcagctcccg gtttgctgat cgcacttttc ttacatcatg
     4021 tgaaagacca gcgtggtatt gcagcgttcg ttgccttgat gtgcgcagtg ggcgcggttg
     4081 ggctctgctt tatgccagcg cacgcgatca cctggactct gcttttcggt tttggttccg
     4141 gcgcaacaat gatactgggg ttgacgttca ttggtcttcg gcaagttctg cgcatcaggc
     4201 ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct gtgggccgcc
     4261 gctaatgggt aaaatacacg atgctaacgg taactggtcc gtaccactga tgggtgttgc
     4321 aatactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca aagaaattcg
     4381 ctaatatccg gtgctatagt gacgtgacaa atcttgcgtg aaagggagaa caaacacgat
     4441 gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt atcgcgtact
     4501 ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg atgagtggga
     4561 taaagaaatc acgccgtcaa cggaactgcg caaagccttt cacggcgaag tcgtcgattt
     4621 tgcaaccttt cgcgagcaat atcgcgcaga actggcgcaa cacgagcaag aaggaaagcg
     4681 gctggcggac atcgccaaaa aacagccgct gaccctgctc tacgcagcaa aaaacaccac
     4741 gcagaaccat gcgctggtgc tggccgactg gctacgtagc ttgtgatttt agtacggcat
     4801 ccggcggtta tttttcacca gccggatggt cccgtcgcca caatgcccat tcatcaatcg
     4861 tttcaccgcc cggtaatttg caattgttgc tgaccccttg cgctgtctgc actggaatga
     4921 gcgtcccgcc cttctgctgg caatagaccg acgccggatt tgccatacca atctgcggcg
     4981 gtttaggtgc ttctggctga gaaggggttg aacaaccagc caggaccagc aagcaaggca
     5041 gaacaaaact gataattttc atttattgat ctcacatatt tatccaagct tagagtatcg
     5101 cggtatcgtt ttgttttgca gcactatttt tattacattc actcaaaaca tattacgtct
     5161 tgtttcatct ttgttgatga tgttttatca tgcctgcaaa gattaaataa tcagcactta
     5221 cccgccgtat cctggagttg ttccgtgtca gatcagatta tcgcccgcgt ctcgcaatcc
     5281 cttgccaaag aacagtcact ggaaagtctg gtccgacagc ttctggagat gctggaaatg
     5341 gtcactgata tggaatcaac ctacctgacc aaagtggatg tcgaagcgcg cctgcagcat
     5401 attatgtttg cccgtaacag ccagaaaatg cacatcccgg agaattttac cgtcttgtgg
     5461 gattactcgt tatgcaaacg cgccattgat gaaaactgct ttttcagcga tgaagtccct
     5521 gaccgttggg gcgactgtat tgcggcacgc aatcttggca tcaccacatt tctgagcacg
     5581 ccaattcact taccggatgg atcattctat ggcacgcttt gcgccgccag cagtgagaag
     5641 cgccagtgga gtgaacgcgc ggaacaggtt ttacagttat tcgccggact gattgcacaa
     5701 tatattcaaa aagaggcact ggttgaacag ctgcacgaag ccaatgctgc gctgattgcg
     5761 caatcgtata ccgactcgtt aaccgggcta ccgaatcggc gggcgatttt tgaaaatctg
     5821 acgacgctgt tttccctcgc ccggcatctt aaccataaga taatgatcgc gtttatcgat
     5881 ctggataact tcaaattaat caatgatcgt tttggtcata atagtggcga tctgtttctc
     5941 attcaggttg gcgagcgcct taatacgctc cagcaaaatg gcgaagttat tggtcgtctc
     6001 ggtggtgatg agtttttggt tgtttcacta aacaacgaga atgcggatat ttcgtcgctg
     6061 cgagaacgta ttcaacagca aatacgtgga gaatatcact taggtgatgt tgatttgtat
     6121 tatcccggtg ccagtcttgg catagtagaa gtcgatcctg aaacaaccga tgcagacagt
     6181 gccctgcatg ctgccgatat tgcgatgtat caggagaaaa aacacaaaca gaaaacacct
     6241 tttgtcgcgc atccagcgct acattcctga ggcgtattca catccttttg attggtgata
     6301 acatgcgaat cggtattatt tttccggttg taatcttcat tacagcggtc gtatttttag
     6361 catggttttt tattggctgc tatgctgccc cgggagcata aagatgaaaa aaacaacgat
     6421 tattatgatg ggtgtggcga ttattgtcgt actcggcact gagctgggat ggtggtaacg
     6481 tcacctctaa aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt taagcgttaa
//
LOCUS       DAJTYM010000163         6381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTYM010000163 DAJTYM010000000
VERSION     DAJTYM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..813
                     /locus_tag="OO575_003752"
     CDS             <1..813
                     /locus_tag="OO575_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6666544.1"
                     /translation="NPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            865..2022
                     /locus_tag="OO575_003753"
     CDS             865..2022
                     /locus_tag="OO575_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6666545.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(1988..2110)
                     /locus_tag="OO575_003754"
     CDS             complement(1988..2110)
                     /locus_tag="OO575_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666546.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2313..3998
                     /locus_tag="OO575_003755"
                     /pseudo
     CDS             2313..3998
                     /locus_tag="OO575_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4036..>6381
                     /locus_tag="OO575_003756"
     CDS             4036..>6381
                     /locus_tag="OO575_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6666547.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDV"
BASE COUNT         1881 a         1383 c         1358 g         1759 t
ORIGIN      
        1 aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac agcagataac
       61 tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga acaactgaag
      121 aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg tgcggttatc
      181 gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac ctatcctggc
      241 ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg taattatgac
      301 aagctgattt ccgcaatgga tttctaccca acagctcttg atgcagccga tatcagcatt
      361 ccaaaagacc ttaagctgga tggtgttccc ttgctgccct ggttgcaaga taagaaacaa
      421 ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt tgatgaggaa
      481 aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga cgattacccg
      541 cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa taacgattat
      601 tcgcttgtct atacagtaga aaacaatcag ttaggtctct acaaactgac ggatctacag
      661 caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca aggcgtggta
      721 agagagttta tcgacagcag ccagccaccg cttagcgagg taaatcagga gaagtttaac
      781 aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc ggatttttcc
      841 gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag ttttcaatgt
      901 aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac tcatgaaaaa
      961 tggatggatg acagcactct gaaagagttc atcaaacaat atatcgaagc gtctggcaat
     1021 caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga ttttttccgt
     1081 aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa tacattacaa
     1141 acgaatggca ttttattgaa taatgaatgg tgtgcctttc tcaaagaaca tgaatttctg
     1201 gttggtatct cgatcgatgg cccccaggag ttacatgacc gttacagacg cagtaattca
     1261 ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc atatcaagta
     1321 gagtttaata cgttaaccgt cattaataac gttaatgtcc attatcctct tgaggtttat
     1381 cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct agaaaccggg
     1441 acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat tgatttttct
     1501 gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg ggttaaaaac
     1561 gatgtgggtg aaattttcat tcgtcagttt gaaagctttg tcagccgttt tttggggaat
     1621 gggcatacaa gttgtatttt ccaggagtcc tgcaaggata atctggttgt tgaaagtaat
     1681 ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg aaacattaat
     1741 aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa acggatttca
     1801 gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc taagcatcgt
     1861 attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa aatccttttt
     1921 tcaaccatgg taccttatat gaacgccatg gttgagttag ctaagaacag agtaccgctt
     1981 taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc tcttttaact
     2041 ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt cctgtcgggt
     2101 gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg cgtcagggca
     2161 ttagcaatac ccttgattat caatagcata accataacca ctgaaaatta aaaatttcta
     2221 tatatttcat attgttaaat gatgcttaaa taaatttcat cagtaaatca ttgccataga
     2281 taatcattgg atggcacaat caattttcgc taatgataac cattcccatt acgttttgta
     2341 tgttgatagc aaagtatctc tgtctgctga agcccttttg gctgcgtaag aataataaaa
     2401 cttcagtgtt gttaatcatt attatcctcg caatgatcct tggcgtggtt aaaattcagg
     2461 tctggttaaa cgactggaat aatgattttt tcaatgcgct aagccagaaa gaaaccgata
     2521 agctttggca gctcgttctt tggttccctg ccctgctggg gatctttgta ttaatatccg
     2581 caaacaaaac atggttaatt aaactactga ctattcgctg gcgcgaatgg ttaacagatt
     2641 actaccttaa tcgctggttt gcagataaaa actactactt cacgcagatc tacggcgagc
     2701 ataaaaatac agataaccct gaccaacgta tcgctgaaga cattctctta ctgatcaaca
     2761 aaacgctcag tctttcattt ggcttcatcc agtcacttag catgctgatc acctttaccg
     2821 ttattctttg gcaaagcgcg ggtacgctct cattcactgt tggtggaaca gaatggaata
     2881 tccaggggta tatggtctat accgtcgtgc tcattgtgat cggtggaact ttatttaccc
     2941 ataaggtggg taaacgtatt cgtccgctta atgtggaaaa acaacgtagc gaagcgacat
     3001 ttcgaactaa tcttgtgcag cataataagc aggcagaact gattgcgcta agcaatgctg
     3061 agtctctaca acgccaggag ttgcgcgaca attttcatac cattaaagag aactggcatc
     3121 gtttaatgaa tcgtcagcgg tggcttgatt actggcaaaa tatttattcg cgctcgctta
     3181 gcgttcttcc ctactttctg ttattgccgc agtttattag cgggcagatc aatctgggcg
     3241 gactgatgaa atcgcgccag gcatttatgc tagtatcgaa caatttaagc tggtttattt
     3301 ataaatatga cgaacttgct gaactggctg cggttatcga tcgcttgtat gagttccatc
     3361 aactcactga atagcgccct acgaataagc ctaaaaattg ccaacatgcg gtacaagtgg
     3421 ctaatgcgag tattcgtacg cctgataata agatcatatt agagaacttg aactttcatg
     3481 tttcgccagg caaatggcta ttactgaaag gctactctgg tgcgggaaaa accacactgc
     3541 ttaaaacatt atcccactgc tggccgtggt ttaaaggtga tatttcttct cctgctgaca
     3601 gttggtatgt gtcacaaaca ccgttaatca aaagcggctt actgaaagag attatttgta
     3661 aagcacttcc cctgcccgta gacgataaat cgttgagcga agtactgcat caggttggtc
     3721 tgggaaaatt ggctgcgcgt attcatgacc acgatcgctg gggagatatt ctttccagcg
     3781 gcgaaaaaca acgtatcgcc ctggcacgat taattttacg acgtccgaaa tggatatttc
     3841 ttgacgaaac tacctctcat cttgaggaac aagaggctat acgcttactg cgtttagtgc
     3901 gtgaaaaact acccacaagc ggcgtcatta tggttacaca tcaacccggt gtctggaacc
     3961 tggctgatga tatttgtgac attagcacgg ttttataaca acaataaaag ccctgctgta
     4021 cggagatata aataaatgaa gcgagttctt attcctggcg tcattttatg tggcgctgat
     4081 gtggcgcagg ccgtcgatga caaaaacatg tacatgtatt tttttgaaga gatgacggtc
     4141 tatgctcctg tccctgtacc cgtaaacggc aacacgcatt acaccagtga aagcatcgag
     4201 cgtttaccga ccgggaatgg caatatcagc gatctgctga gaaccaaccc tgcggtacgc
     4261 atggattcaa cgcaaagtac ctcgttgaac cagggagata ttcgccctga gaaaatctct
     4321 attcacggtg cgtcgcccta ccagaatgcc tatttgattg acggtattag tgcaactaat
     4381 aacctgaacc cagcgaatga gtccgatgcc agtagtgcaa ccaatattag cgggatgtca
     4441 caggggtatt atcttgatgt cagcttactg gacaatgtga cgctttatga cagttttgtg
     4501 ccggttgaat ttggtcgctt caatggcggg gtaattgatg caaagatcaa acgcttcaac
     4561 gctgatgata gcaaggtgaa attgggttat cgcactacgc gtttggactg gttaacatcg
     4621 catatcgatg agaataacaa gagcgcattt aatcaaggtt cttcaggaag tacctatttc
     4681 tctccagatt ttaaaaagaa cttttatacc ttgtcgttta atcaggagct ggctgataac
     4741 tttggcgtta ccgccggttt atcgcgccgc cagtctgata tcacccgcgc ggattatgtt
     4801 tcgaatgacg gcattgtcgc cggtcgggca cagtataaaa acgttatcga tactgcattg
     4861 agcaaattta cctggtttgc cagcgaccgc tttacccacg atttaacctt aaaatatacc
     4921 ggctccagcc gtgattataa taccagcacc ttcccgcagt ctgatcgcga aatgggtaat
     4981 aaatcctatg gtctggcatg ggatatggat acgcaactcg catgggccaa actacgtacc
     5041 accgttggtt gggatcatat tagtgattat acccgtcacg atcatgacat ctggtacacc
     5101 gaactttcat gtacatatgg tgatattaca gggcgttgta cccgtggcgg attaggacac
     5161 atttcccagg ctgtagataa ttacaccttc aaaacacgcc tggactggca aaaattcgcc
     5221 gtgggtgatg tttcgcatca accctacttc ggcgcggaat acatctattc cgatgcatgg
     5281 actgaacgcc ataaccagtc tgaatcctat gtgattaatg ctgcaggaaa gaaaactaac
     5341 cataccattt accataaagg taaaggcagc ctgggaattg acaactacac gctgtatatg
     5401 gcggatcaca ttagctggcg gaatgtgtcg ttaatgcccg gtgtgcgtta tgactatgac
     5461 aactatctgt caaaccacaa tatctccccg cgctttatga cggaatggga tatttttgct
     5521 gatcaaacct caatgattac cgccggttat aaccgttact atggcgggaa tattcttgat
     5581 atgggattac gtgatatccg caatagctgg acggaatcgg tatcaggtaa taaaaccctg
     5641 acgcgttatc agaatttgaa aacgccttat aacgatgaac tggcaatggg attgcagcaa
     5701 aaaatcgata agaacgttat tgcacgcgca agtgaagcgc atgatcaaat cagcaaaagc
     5761 agtcgtaccg acagcgcgac taaaaccacc attactgaat ataacaacga tggcaaaacc
     5821 aaaacgcatt cgtttaacct cagttttgaa ctggccgaac ccctgcatat ccgccaggta
     5881 gatattaacc cgcaaattgt ctttagctat atcaagagca agggcaactt gtcgttaaac
     5941 aatggttatg aggagagcaa taccggtgat aaccaggtgg tttataacgg taatctggtc
     6001 tcttacgata gcgttccagt ggcagatttt aataacccat taaagatctc cttaaacatg
     6061 gatttcacgc atcaaccgag cgggttagtg tgggcgaata cgctggcctg gcaagaagcg
     6121 cgtaaagctc gcattatcct tggtaagaca aatgcgcaat acatcagcga atattcagat
     6181 tacaagcagt atgttgacga aaaactggat agcagcctga cctgggacac ccgcttgtcc
     6241 tggacgccac aatttctgaa acaacaaaac ctgacgatca gtgccgatat tctcaatgta
     6301 ctggatagca aaaccgctgt tgatacaacg aacaccggtg tggcgaccta cgccagtggc
     6361 cgtactttct ggcttgatgt c
//
LOCUS       DAJTYM010000164         5983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTYM010000164 DAJTYM010000000
VERSION     DAJTYM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..393)
                     /locus_tag="OO575_003757"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO575_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO575_003758"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO575_003758"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6666548.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO575_003759"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO575_003759"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6666549.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="OO575_003760"
     CDS             5797..>5983
                     /locus_tag="OO575_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6666550.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1770 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAJTYM010000165         5754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTYM010000165 DAJTYM010000000
VERSION     DAJTYM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            97..1449
                     /gene="celF"
                     /locus_tag="OO575_003761"
                     /gene_synonym="chbF"
     CDS             97..1449
                     /gene="celF"
                     /locus_tag="OO575_003761"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6666551.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1462..2220
                     /gene="chbG"
                     /locus_tag="OO575_003762"
     CDS             1462..2220
                     /gene="chbG"
                     /locus_tag="OO575_003762"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6666552.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2267..4528)
                     /gene="katE"
                     /locus_tag="OO575_003763"
     CDS             complement(2267..4528)
                     /gene="katE"
                     /locus_tag="OO575_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6666553.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4711..4974
                     /gene="cedA"
                     /locus_tag="OO575_003764"
     CDS             4711..4974
                     /gene="cedA"
                     /locus_tag="OO575_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6666554.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5251..5748
                     /locus_tag="OO575_003765"
     CDS             5251..5748
                     /locus_tag="OO575_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666555.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGS
                     EFRIRDINENFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEKNYIHPAGSIFA
                     AIQKK"
BASE COUNT         1455 a         1244 c         1480 g         1575 t
ORIGIN      
        1 acctgacaat tcacatatct gtcctgttgc tggattattt atgtccgggg gggcagatat
       61 gccagatatc agtattctgt actgaaggga gaaattatga gccagaaatt aaaagtcgtc
      121 actattggtg gcgggagcag ctataccccg gagttactgg aaggatttat taagcgttat
      181 cacgaattgc cggtcagcga attatggctg gtggatgtcg agggtggcaa agctaaactg
      241 gatattattt tcgatctctg ccaacggatg attgataacg ctggcgtccc gatgaagctt
      301 tataaaacgc tggattgccg cgaagcattg aaagatgctg atttcgttac tacccaactg
      361 cgcgttggcc aattaccggc gcgtgaactg gatgaacgta ttccattaag tcatggttat
      421 cttggtcagg aaaccaacgg cgcgggcggt ttgtttaaag gtctgcgtac cattccggtg
      481 atttttgaca tcgtaaaaga tgtcgaagaa ctttgtccga atgcatgggt gattaacttc
      541 actaacccgg cgggaatggt tactgaagcc gtttatcgtc ataccggatt taaacgcttt
      601 atcggcgtgt gtaatattcc gatcggcatg aagatgttta ttcacgatgt tctgatgctg
      661 aaagacagcg atgatttatc tatcgatttg tttggcctca accatatggt gttcattaag
      721 gatgtgctgg taaatggcaa gtcgcgcttt gccgaattgc ttgatggtgt ggcatccggg
      781 cagttaaaag tatctggcgt taaaaatatt ttcgatctgc catttagcga aggcttaatt
      841 cgttctctga atctgttgcc gtgttcttat ttgctttatt acttcaagca aaaagagatg
      901 ctggctattg aaatgggcga atactacaaa ggcggcgcac gagcgcaggt agtacagaaa
      961 gtcgagaaac aactttttga gctgtataaa aacccggagt tgaaagttaa gccgaaagaa
     1021 ctggaacagc gcggtggggc ttattactct gatgcagcgt gcgaagtgat caacgctatc
     1081 tacaacgaca agcaagctga acattacgtt aatatcccgc atcatgggca tattgataat
     1141 attccggcag actgggcggt agaaatgacc tgtaagctgg ggcgcgatgg cgcgacgcca
     1201 catccgcgca ttacgcattt cgatgataaa gtgatggggc tgattcacac cattaaaggc
     1261 ttcgagattg ctgccagcaa cgccgcactt agcggagaat ttaacgatgt gttactggcg
     1321 ctaaacctta gtccgttggt gcattccgat cgcgatgctg agctgctggc acgcgagatg
     1381 attctggcgc acgagaaatg gctgccaaat tttgccgact gcatcgcaga gcttaaaaaa
     1441 gcacattaac cgaggctgat tatggaacgc ttactgattg ttaatgccga tgattttggc
     1501 ttaagcaaag gtcagaacta cggcattatc gaggcctgtc gcaatgggat tgtcacatcg
     1561 acgacggcgc tggtgaatgg gcaggctatt gaccatgcgg tgcagttgag tcgtgatgaa
     1621 ccgagtctgg ccatagggat gcactttgtc cttactatgg gcaagccact gacagctatg
     1681 ccggggttaa cccgcgatgg tgtgctggga aaatggatct ggcagttggc agaagaagat
     1741 gctttaccgc tggaagaaat tactcaggag cttgccagtc agtatttgcg tttcattgag
     1801 ctatttggac gcaaacctac gcatcttgat agccatcatc atgtgcatat gttcccgcag
     1861 attttcccga ttgttgccag gtttgcggct gaagagggga ttgcgttgcg catcgaccgt
     1921 cagccgctat ctaacgctgg tgatttaccg gctaatctgc gcagttcgca gggattcagt
     1981 agcgcgtttt atggtgaaga gattagcgag gcactgttcc tgcaagtgct ggatgacgca
     2041 agccatcggg gtgatccttc gctggaggtg atgtgtcatc cggcgtttat cgataatact
     2101 atccgtcaga gtgcttactg tttcccacgg ttaacggagc tggaagtgct gacatcagcg
     2161 tcgttgaaat atgcaattgc tgagcgtggt tatcggttgg gtagttatct tgatgtgtaa
     2221 gtaaggtggt aaggtcactc cccaggggac gtgaccttat ccatatttac gccggaattt
     2281 tgtcaatctt aggaatgcgt gaccacacgc ggtgttctgc catcagcgtt aacagttcat
     2341 ccataaaact accgtcggtg ctgtcagctt ccacaatccc ttcttcaccc tggtcagcta
     2401 ccttgattgt tgctttaaac ttgcgcgcgt ctcccgccag cgcaatcggt ttaaggtgtt
     2461 tgtaggcttc catcagatag tagttggcat cgccgttgtc agcgatatcc gcgatattgc
     2521 cgcaagggac aatgaccgca tcgaccgtca gcgaaggtgc accggcaaag gtagcggcta
     2581 taggcaacac cgtaccgtca tccgcagtca cttcacccat tcgggagtag agcagtttgg
     2641 catgaacgcc tttggccttc atggccttga gaatggccag aaggtctgcc gatctcactt
     2701 catcattaag taaaatcgct accacgcgac ctttcacatc accgtcagga atggcgtaca
     2761 aacttaagga tggatccttt ttcagaccgt tgacgtccgg aggtggggtg atattcagct
     2821 ggtcgtcagt cagttcgata ccgagatttt tcgccaccgc ctgggccaga gtgagatcaa
     2881 tatgcgccag ctggtcaaca acgcgctcac gaatatacgg acgaacgact ttgcttaact
     2941 caaaactgaa accatcgaca atatggcgct gctcgaatgg cgtctgactt agccagaaca
     3001 gacgcggatg ggaataatat tcgccaaacg atgggctgcg ctcgcgaact ttattgcctt
     3061 ccacgcgctc ttggtatgat tcaaaaccgc cgcgtttcgg ccccggtggt gtttcgcgcg
     3121 gccagttatc gttgatcgag ttcggttcat aattcgccgg gttagtgtcg atccccatac
     3181 gatgcatgcc gtcacgctgg aaattatggt aagggcaggt cgggcggtta atcggaatct
     3241 catggaaatt cggcccacca agacgactga tttgtgtatc ggtataggag aacaaacgtc
     3301 cctgcaacag gggatcgttg gtgaagtcca gaccaggaac aatatgacct ggatggaatg
     3361 ctgcttgttc gttttcggca aagaagttat ccggattgcg attgagcacc attttgccga
     3421 cacgctgaac gggcaccagt tcttccggga tcagtttggt tggatcgaga agatcgaagt
     3481 cgaacttgaa ttcatcttct tcaggaatca actggaagcc cagttcgtat tccggaaaat
     3541 cgcctgcttc aatggcttcc cacaactcgc ggcggtggaa gtccgggtca cgtccggtga
     3601 gtttttgtgc ttcatcccaa acgagtgagg ctttacctgc cagtggtttc cagtggaaac
     3661 gtacaaacgt tgccttccct tcggcattaa tcaggcggaa ggtgtgaata ccgaagcctt
     3721 ccatggtgcg gtaactgcgg gggatgccgc gatccgacat cgcccacatc acgttgtgca
     3781 gagtttcagg ttgcagagaa acataatccc agaaagtatc gtgggcactt tgcccttgtg
     3841 gaattgccca gtgcggttct ggttttaccg catgaacaaa atcggggaat ttatgcgcat
     3901 cctggataaa gaagattggc gtgttattgc caacgaggtc aaaaataccc tcttcagtat
     3961 agaacttggt ggcaaaacca cggatatcac gcacggtatc ggcagagcca gcgccaccct
     4021 gaacggtaga gaacctaaca aatactgggg tgattttgtt cggatctgag aggaaatccg
     4081 ctttggtgat atcgcttaag cttttatatg gctggaaata accgtgagct gctgagccgc
     4141 gtgcatgaac aatacgttcc gggatgcgct catggtcaaa gtgggtgatt ttctcgcgca
     4201 gaataaaatc ttccagcagc gttggaccac ggttaccggc acgcagtgag ttttgatcat
     4261 cggcgatgcg cacgccctga ttagtggtca gcgcataatt ttcactgcct ttgcgtacgt
     4321 cttccagaga attaagtttt tcgttacgtg tatcaggggc tttcaggctc ccaggggcgg
     4381 taggttgtgc acctggcggt gttggttcag ccgctggacg atgagatccg tcctcaggtg
     4441 ccagtgagtc catccccggt ttcgcttcgc tggaatcgtg tagtggtgac tggtgctgat
     4501 gtgggttctt ttcgttatgt tgcgacattg aactcgtctc cttaatttat tactgaaagg
     4561 gccgctgttt tggtcaaaaa actctctaac tatagaccac cagccagatc ccgcttcgga
     4621 gactattctg gaaacggtgt tatgaaatac acgcaaagcg ggacgtacag gggctaaatc
     4681 ggctaaacta cagtttttct aatttttaat atgcgtttag tgaagccagt aatgaagaaa
     4741 ccgctccgcc agcaaaaccg ccagattatt agctatgtcc cacgcacgga acccgcgccg
     4801 ccagaacatg cgataaagat ggattcgttt cgtgatgtct ggatgctgcg tggcaaatat
     4861 gttgcgtttg tactgatggg agagtcattt ctgcgctcac cggcgtttac tgtgcctgaa
     4921 tctgcccaac gttgggcaaa tcagatccgc caggaagggg aagtgactga gtaaggatta
     4981 catacctccc ggttttgccg ggagttacag gacctgaaat tctgcatttc gttgctacat
     5041 tcattcaaac cacctgtaac aaaaagctgc ctttgacgag ctattgctat aaataagaga
     5101 tcttttttct ttaattattt atttatttta aaatggttaa gttttgctaa tcatgatgag
     5161 ttatttttga tagttcatcc tgtggcgtcg ctcttaaact tatattgtaa atgacttaga
     5221 tttaaataag ataataattt tgttaacaat atgaggcggg agatgttttc tacaaactta
     5281 atacagagta attatggtga tttaaatatt aaaagcctgg ctttcgactc ttttaaagaa
     5341 aggcttgagt ctaccatggc ggcactcact ttctttatat caacgggtca atgtgactgc
     5401 gatgaaatag cagagtcgaa cttcaatcgt atgattgcct atatgtctaa cattaactat
     5461 gatgcctcaa agctaggagc gccagcactt agttttgata cgtatctgca ggacaatgtt
     5521 aaatatagag tgataattaa taatctttat ggcagtgagt ttcggataag agatattaat
     5581 gagaattttg ttgggatgga ttttacctgt gaattccgac ccgaaaaaat gacgaatttg
     5641 atcaatataa aaaataggtt ggttattcat tatttaagga ctatatacta tgaaaaaaat
     5701 tatatacatc ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtct
//
LOCUS       DAJTYM010000166         5653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTYM010000166 DAJTYM010000000
VERSION     DAJTYM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            188..460
                     /locus_tag="OO575_003766"
     CDS             188..460
                     /locus_tag="OO575_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6666556.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(426..833)
                     /gene="nudG"
                     /locus_tag="OO575_003767"
     CDS             complement(426..833)
                     /gene="nudG"
                     /locus_tag="OO575_003767"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6666557.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            920..1540
                     /locus_tag="OO575_003768"
     CDS             920..1540
                     /locus_tag="OO575_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6666558.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1549..2856)
                     /gene="ynjE"
                     /locus_tag="OO575_003769"
                     /pseudo
     CDS             complement(1549..2856)
                     /gene="ynjE"
                     /locus_tag="OO575_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(2923..3576)
                     /locus_tag="OO575_003770"
     CDS             complement(2923..3576)
                     /locus_tag="OO575_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6666559.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3576..5047)
                     /locus_tag="OO575_003771"
                     /pseudo
     CDS             complement(3576..5047)
                     /locus_tag="OO575_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5020..5653)
                     /locus_tag="OO575_003772"
                     /pseudo
     CDS             complement(5020..5653)
                     /locus_tag="OO575_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1338 a         1530 c         1490 g         1295 t
ORIGIN      
        1 gtcatgtgct tttgcagttt tcgactatac catcaagaat gtgacggtaa gcaagaaaaa
       61 aatgacccag gaaagcaaat taataacgag agtaatctca taaccaaatg cgataactca
      121 gacgctaaag ttttgccacc cggctaccgt tatcagtagc agagacaata acgttaagga
      181 gttccctgtg agtcgagcat tgttcgccgt agtattagca tttccattaa ttgcactggc
      241 taacccgcat tatcgtccgg acgtggaggt aaacgtaccg ccagaagtgt tcagttcagg
      301 cgggcagtca gcgcagccct gtactcagtg ctgcgtttat caggatcaaa attattctga
      361 aggggcggta attaaagccg aaggaatact gctgcaatgc cagcgtgatg acaaaaccct
      421 tagcactaat ccgctgctct ggcggcgcgt aaagccataa acgcctctaa taatggaatg
      481 tcagcagggg ccagcggata ttgcagcgcc tcttcaggtg agcaccagac cagcgcctga
      541 tgttcatgtg cctgtaacgt cccgtggaag tcgggtacgt gccaggcatg aagatggata
      601 atccggcccg aaacttctcg ctgatggctg gcaacatact cacccacagt tgcttcgatg
      661 tccaattctt cgtttaactc acgcaccagc gcctgctgct ggctttcatc cagctcgact
      721 ttacctccgg caaactccca taatcccgcc tgatcgctct gggcggggcg ttgcgcgagt
      781 aaaattttgc catcacgttc aatgatggcg gcaacaactt caatcatttt cataaattac
      841 acacgggcag ataagcgaaa aatgccataa tatccgcact gttgctcaat cagaaaggct
      901 ttatatttaa ggagtgcctg tgctagaccg ccatcttcat ccccggatta aaccgttgtt
      961 gcatcagtgc gtgcgggttc ttgataaacc gggcattacg ccagatggtt taacattagt
     1021 gggatttgcc atcggcgtgc tggctctgcc gtttctggcg ctgggctggt atctggcggc
     1081 gctagtcgtc attttgttga acaggctgct tgatggtctg gacggtgcgc tggcgcggcg
     1141 cagagggctt accgacgcgg gcggctttct tgatatttct ctcgattttc tcttttacgc
     1201 gctggtgccg tttggcttta ttcttgctgc accggagcaa aatgcgctgg caggcggctg
     1261 gttgttgttt gcgtttatcg gcacgggcag cagttttctc gcctttgctg cactggcagc
     1321 aaaacatcag attgataacc ccggttatgc acataagtcg ttttactatc tggggggatt
     1381 aaccgaaggc acagaaacga tcttactgtt tgtgctggga tgtttattcc ctgcatggtt
     1441 tgcgtggttt gcgtggatct tcggtgcgtt gtgctggatg acgaccttta cgcgggtgtg
     1501 gagtgggtat ctgacgctga agtcgctcca gcgtcagtaa ttgcaatgtt atttgctact
     1561 gcccgggccg cgttcaccgg ttgcaaccgg attttttgga tcgctgctcc attcgtacca
     1621 gccgccgtca tacacggaaa cattcttcca acccatggcg cgtgcgtaca taaaggtttc
     1681 ggacgcgcgc cagccggtgc cgcagtagaa tgaaacttgc tgatctggtt tgatattcca
     1741 tgctttccac atagcggtaa tatcatcggc gctacgcatg gtgccatccg ggttatagaa
     1801 atcttccata tgcgtcgagt cgctaccagc gtgtccccaa cgtgctccgg ctatttcacc
     1861 ttttggttta atatagctgt aaccgctggt cgtaccgata aattctggcc acgaacgaat
     1921 gctcaccagc gatgcatcct ggcgatgcag cagtccacgg gcctgttcca tatcgagcat
     1981 caactgcggt tgtgccggga tcttcacgcc gaaatccggt tccgctttca ctttcggtgg
     2041 cgttcctcgc tcaacaggca gtcccgcgtc ggaccaggtt tgccagccgc catccagcag
     2101 acgcacatct ttcacgcctg cgtagagcat aatttgcgcc acacgcgctc cagcgtatac
     2161 gtcacgcccg tacagaatga ccgtggtgtc atggcgaatg ccgtgttttg ccagcatcgc
     2221 ttttagttgt tcatcagaaa ctttgttcca cagcggttcg ctttccactt cgttggtatc
     2281 gatgtagtca gcgccaggaa tatggctgat aaggtaaagc ttaggagcgc cctaggccgc
     2341 ttcaatgact ttccagtcac cgacaggttt cgccgtaacc tctttacctt gttgcaggtc
     2401 atgcagccat tgcggataaa ccagctgctc aaagtgcggc agtttttgta gacgggaagg
     2461 ttcgcttagc gcgtcactca gaatggagat atgcgtaaaa cctgcttttt gcagtcgcgt
     2521 tttgacggcg tcaacatctt tgccattacc gtacagcgcc accggggcat cggctttcag
     2581 gttatgttgc ttgatccacg cgttgagctg ttcggtgctc attttgtcca gccagctagc
     2641 agagaggttt aaggcggcag gttcatgacc agaagggcca tttaaggttt gtggccagcc
     2701 gttataaaac gcgctggggc gagtatctat cgctttgcca ttttgttgct gaagttggtc
     2761 aagtgtaaga ggcttcgcca gttcagcggc ccacgaagaa gcgcaagcca gccctaaagc
     2821 cattgccagc gcggtcattt gagaaacacg tttcatcgaa aatcccgaca ttgtagtttt
     2881 gagtgcgcca ttctgaactg acgcattgag aaaaactttg cgttatcgca gtttgttgta
     2941 attttctgac cactgcgcca tatccagaac agaactatca gcaggaacat cctggagatc
     3001 gtgcgttacc tgaacgacgg ggatcgccag ggcgcgaact tcgctgaaca cccactggcg
     3061 aaaattatcg cgcagagcca catcaagacg gctgaatggc tcatccagga gtaacgcttt
     3121 tggctgggcg agaagggcgc gtagtagagc aacgcgcgct cgctgaccgc cagacaaagt
     3181 ggcaggatcc tgatggaaag ctccgtcaag gcctgaacgc tcaagtgcat cattcacggc
     3241 attacgtcgg gcattcccct taagtgtcgc cggtagcgcc agcagtaaat tttgcccgac
     3301 gctgaactgg tcaaataaca gtgcatcctg aaaaagaatg ccaatctgac gctgtgcggt
     3361 gggtagcatg tcaatccgtt gctcattgag ccatagctca cctgtacaag aaaactgtcc
     3421 ggccagtgca ccaatcatcc atgaaaacag agtggatttt ccacagccag acggccccat
     3481 taacgtgacg atgtcacctt tatcaaccgt aaagttaacg tttgtcagca agcggctttc
     3541 tggtaaacgt agcgaaacat ttttcacgca gagcattagc ggattccttg tctgacataa
     3601 cctacccatt ttgcgactaa cgcggtcagg gcaaaaataa taagcggtaa caacagttgc
     3661 cataacgcct gggcggcgag aataccgttg ctgccgccgc tgcttaatgc caccgcctca
     3721 gtggtgagcg tcggaaaacg ccccgcgccc agccacagcg tcggcatata ctgcgcaata
     3781 ctgactgaaa atcccaccgc gaaggcaatc agcacagggc gcaacatgag tgggcatttc
     3841 acgtaaaaga agattttggc ccgcgaccag cccagtgttt gcgcaatcaa aattaaccgt
     3901 gaatcaatgc gctgccaggc gggttgcagg ataaacagca tccacggcat tacccacagc
     3961 agatgccccc agaccaccgc cgtccagctt ccatccaggt tcagccatag cgccagcgtg
     4021 tactggcccg ccaccagtgg cagagcgggc aatagaatgg gcaaccatag ccacaactgg
     4081 cgacgctgtg ccccccattc cagccacagc agtaacagga gcaaagcgat gaatgttgct
     4141 accagcccca ttgtcagact gttgatgagt gcttcactat tgatcgtcga ctgatccgcg
     4201 aggatcgcca gcagaaccac acacagcaca ccggttaagg gtaaaaaact cgccagcgta
     4261 gtgcctggta ataaaggtgt ggcaggcttg cgaatgccat ctacgcgggg aatagtgcgc
     4321 cgccagctgc gccaaaacgt aggcggcgag taacagcatc agcagcagac tggcgagtgc
     4381 gcctttcgtt tgttgatccg cgtcgccctg agttaaccac tgccagctaa ttactgccag
     4441 cgtcggtgga ttacctggcc caagaataat cgccacatcc acgaccgaaa gcgaccaggc
     4501 aacaatcgcc agcatcgcca ttgccagcgg ctgtagccca gcgaatccag cacaatgacc
     4561 tgctgcaaca gccgtttttc gctcaatact gccactaaga tccacagcag aaatgcgctt
     4621 tctttcactg caagggtgag gcccagaccg atccaaatcg gtccattggc ggagtgaaat
     4681 acgggaaata gtcatatagc agccctccgt cagcaaagag cagcagggcg ctggtggcaa
     4741 aagccacatg gggaatggcg agcagccacg gcagacgggc gcacatacgc tgccatttcg
     4801 gcccaggcca tagcgccaca atcaccagca gggcaatcaa caatgccccg accgccgcga
     4861 tggttgtcga caccagcgtt gccagtaatg cctgcggtaa ctgtggatcg gcaaataacg
     4921 cctgccagcg tgtcaacgac aacgcaggcg atatcagcgt gagagctgcc gggatcagcg
     4981 gtgcataaat caccgccacc atcgcccaca gcaaaaagat taatgcgtac cgtaacggcg
     5041 taaccattct tgttccagtg cattcaccca tcctgcgtgc ggttcagcca gtaccggcgg
     5101 cagatcctgc ggcattcttg attgcaatat ttcgcgctgc ccgtcaggca gtttttgcgg
     5161 atcgagaaca gaaggatcgc cccagacaac gggatctgct ttacgcagtt gcgcatcggg
     5221 tgagagcagg aaattggcaa ctaccttcgc cgcagcactg gcattcgcgt tggctggaat
     5281 ggtgacgaaa tgcacgttgc caatcatccc ctcgcgaaag ccaaaactgt aactgcttgc
     5341 cggcaaatcg ccgctggcga ttttttgctg cgcatgtgcg gggttaaagg tcagcgacag
     5401 gcgcaatgtg ccggctttca gcagagcatc catccgcgcg ggcgaaggcg ggaaatcctt
     5461 tccttcgcgc cacaaatacg gatgcagtgc atcaagatat tgctacaagg gcgcagtgac
     5521 acgggcgaaa gtcgcatcgt ccggcgcttc ttttaatgcg gcgggatctg gcgtcagcat
     5581 aatcagcaac tgttcaagaa acgccgtgcc ggtaaagtcc ggtgggcgcg gataagtaac
     5641 cgtgccggga ttg
//
LOCUS       DAJTYM010000167         5600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTYM010000167 DAJTYM010000000
VERSION     DAJTYM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..411
                     /locus_tag="OO575_003773"
     CDS             <1..411
                     /locus_tag="OO575_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6666560.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OO575_003774"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OO575_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO575_003775"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO575_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6666561.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1332 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc
//
LOCUS       DAJTYM010000168         5491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTYM010000168 DAJTYM010000000
VERSION     DAJTYM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(106..1815)
                     /locus_tag="OO575_003776"
     CDS             complement(106..1815)
                     /locus_tag="OO575_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6666562.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="OO575_003777"
     CDS             complement(2247..2966)
                     /locus_tag="OO575_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6666563.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3091..3647)
                     /locus_tag="OO575_003778"
                     /pseudo
     CDS             complement(3091..3647)
                     /locus_tag="OO575_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3631..3889
                     /locus_tag="OO575_003779"
                     /pseudo
     CDS             3631..3889
                     /locus_tag="OO575_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3998..4576)
                     /locus_tag="OO575_003780"
     CDS             complement(3998..4576)
                     /locus_tag="OO575_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666564.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(4997..5234)
                     /locus_tag="OO575_003781"
                     /pseudo
     CDS             complement(4997..5234)
                     /locus_tag="OO575_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1555 a         1187 c          951 g         1798 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgcc atattcagtt tcccatagtg
     3121 gaacatcact cccagcttaa agcgctcccg gttgcggtag cccttggctt ttattctcag
     3181 cagcctgatc ttgctgttca gtgcttccgc atttccgttt gatacgccat ggcgcatggc
     3241 gttcaggata ccgtacaacc ttttccctat cgttttagcc acattcttca tcatggggac
     3301 gtcactgttg gccgcagcca tctctgccag tcgttgcgtc tttcctcgct ccatggtctg
     3361 ctccagatat cctttgccag ttctttcagc gcccagcaca ggctcgtcag cttcatctgc
     3421 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
     3481 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
     3541 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
     3601 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
     3661 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
     3721 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
     3781 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
     3841 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
     3901 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
     3961 gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct
     4021 ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata
     4081 aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa
     4141 taaggccgca catatccgta atatatttgt caatgctgct accatttgca tggtaatatg
     4201 tcatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa
     4261 caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct
     4321 cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta
     4381 tcttggcttt gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt
     4441 tatgacatgc attcagctcg ttaattttac ctgataaaac tgcttttctt accacatcgt
     4501 tgaactcatc gatagtgtgt tttttattat atatatcatg aaaaatatag cctaaagact
     4561 taaacgagac tttcatttta tattctcctt aaatattaac aggatatata tatcacattt
     4621 tcctctatat tttcagaaac cagctatcat tttccccatt actggtacgt cgcttcagat
     4681 atttccgcag caagcgataa ccctgcgcac tatgaggatg ccattcatta taatgtttga
     4741 acaccttcgc gagactactt tactgctgtt aacacgtcag gttcgaacat aacactgatt
     4801 taatcatatt tcatcgtttt gacgaagttt tctgccattc cattttgcac cgccgtgtgt
     4861 ttgggggcaa ggccgaccat ctgtgtttca ttcgccagat ataagtgatg agatggcatt
     4921 ctccttcctt cacggcagaa gcattttgac cgtttagggg atgcggacga ttttcatgcc
     4981 tgctaagata ctttcgttac tgatgctgtc cgttttccac catttcatca tcactgattg
     5041 gcgttgtgtt tttggtcagc actccagctt tacgtttctc ttttaaccgg taactctctc
     5101 ctttgatatt cagcgtgtag cagccgatct aggatcgctg ttgccagcac gtgatctccg
     5161 aacatttctc cccagtctgc gacccctttg tttgacgtca gtatgatgct cgctttttca
     5221 tatcgacagt tcatcgatag gaatttaaat ccccaaaaga ttaaaaaagc accgaagaat
     5281 gcgcgtttat tcaacacaac ctgatcatca agtaatcggt atagacactt aatattgctt
     5341 acattgattg attcaatgta tctctgatgg ttttttgctc cgttcccctc aacatggacg
     5401 caacttcccc tctgcgactc tcagccgcac caccgtattc gagccagcgg ctcatgcatc
     5461 ataacctgct ctgccagacg gtaacccctc t
//
LOCUS       DAJTYM010000169         5391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTYM010000169 DAJTYM010000000
VERSION     DAJTYM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..279)
                     /locus_tag="OO575_003782"
     CDS             complement(1..279)
                     /locus_tag="OO575_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666565.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO575_003783"
     CDS             220..405
                     /locus_tag="OO575_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666566.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO575_003784"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO575_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6666567.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO575_003785"
     CDS             complement(1254..1670)
                     /locus_tag="OO575_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666568.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO575_003786"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO575_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OO575_003787"
     CDS             1837..2862
                     /locus_tag="OO575_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6666569.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OO575_003788"
     tRNA            complement(2915..3004)
                     /locus_tag="OO575_003788"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OO575_003789"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OO575_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6666570.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OO575_003790"
     tRNA            3996..4071
                     /locus_tag="OO575_003790"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5391
                     /locus_tag="OO575_003791"
                     /pseudo
     CDS             4233..5391
                     /locus_tag="OO575_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1442 a         1280 c         1257 g         1412 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacaggtag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg g
//
LOCUS       DAJTYM010000170         5316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTYM010000170 DAJTYM010000000
VERSION     DAJTYM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..415
                     /locus_tag="OO575_003792"
     CDS             <1..415
                     /locus_tag="OO575_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6666571.1"
                     /translation="VEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKDYLL
                     YKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEALHRQ
                     VVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            481..729
                     /locus_tag="OO575_003793"
     CDS             481..729
                     /locus_tag="OO575_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6666572.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            794..1162
                     /locus_tag="OO575_003794"
     CDS             794..1162
                     /locus_tag="OO575_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6666573.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            1256..1909
                     /gene="nfsB"
                     /locus_tag="OO575_003795"
     CDS             1256..1909
                     /gene="nfsB"
                     /locus_tag="OO575_003795"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6666574.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            2017..3264
                     /gene="ybdG"
                     /locus_tag="OO575_003796"
     CDS             2017..3264
                     /gene="ybdG"
                     /locus_tag="OO575_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6666575.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(3332..4708)
                     /gene="pheP"
                     /locus_tag="OO575_003797"
     CDS             complement(3332..4708)
                     /gene="pheP"
                     /locus_tag="OO575_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6666576.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4809..>5316)
                     /locus_tag="OO575_003798"
     CDS             complement(4809..>5316)
                     /locus_tag="OO575_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCR6666577.1"
                     /translation="LMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLWWMGFHLS
                     VATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALYHGAVLRV
                     RPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIPAAYKLMW
                     LHRHRVRN"
BASE COUNT         1352 a         1395 c         1300 g         1269 t
ORIGIN      
        1 ggtggaggtt cgggtgatgg ataccccgtt aacccttagc cacgcggtaa atatggcggg
       61 attaattcag gccaccgccc actggttact gacggaacgc ccgttcaaac atcaggagaa
      121 agattacctg ctgtataaat tcaaccgttt ccaggcctgt cgctatggtc tggaaggcgt
      181 cattaccgat ccgcacactg gcgatcgtcg accgctaacg gaagatacct tgcgattgct
      241 ggaaaaaatc gccccttcag cacataaaat tggcgcatcg agcgcaattg aagcactgca
      301 tcgccaggtc gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg
      361 cggctcgctg attgggctgg tgaaaaagca ttgtgagatc tgggccggtg actaagccgg
      421 gaattgcctt acagaagaat atccctgaca atggtgtttt aacttacatt taacaatgtt
      481 atgaaacacc ctttagaaac cttgaccacc gcagcaggca ttttgctgat ggctttcctc
      541 tcttgcctgc tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa actggtgacc
      601 atgttccatc tgatggatct tagccagctt tacactttat tgttttgtct gtggttttta
      661 gtgctgggcg ctattgagta ttttgttctg cgctttatct ggcgacgctg gttctcgctg
      721 gcggattaaa cgtcgattat cgccgaactt cgcggcatac tttgcttatt ctccttcgcc
      781 taaaggaacg tttatggata agcaatcact gcacgaaacg gcgaaacgcc tagctcttga
      841 gttacccttt gtcgagcttt gctggccttt tggcccggag ttcgatgttt ttaaaattgg
      901 cggcaagatt tttatgctgt cgtcggagct acgcggcgtc ccctttatca atctgaagtc
      961 cgatccacaa aaatccctgt taaatcagca aatataccca agcattaagc cagggtatca
     1021 catgaataaa aagcactgga tttcggtgta ttccggcgag gaaatctccg aagcgttact
     1081 tcgcgatctg atcaacgatt cgtggaatct ggtggttgat ggtctggcta aacgcgatca
     1141 aaaaagagtg cgtccaggct aaagcgggaa tctatagcgc atttttctcg cttaccattt
     1201 ctcgttgaac cttgtaatct gctggcacgc aaaattactt acacatggag tctttatgga
     1261 tatcatttct gtcgccttaa agcgtcattc cactaaggca tttgatgcca gcaaaaaact
     1321 taccccggaa caggccgagc agatcaaaac gctactgcaa tacagcccat ccagcaccaa
     1381 ctcccagccg tggcatttta ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa
     1441 atccgctgcc ggtaattatg tgttcaacaa acgtaaaata cttgatgcct cgcacgtcgt
     1501 ggtgttctgt gcaaaaaccg cgatggacga tgcctggctg aagctggttg ttgaccagga
     1561 agatgccgat ggccgctttg ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa
     1621 gttcttcgcc tatatgcacc gtaaagatct gcatgatgat gcagagtgga tggcaaaaca
     1681 ggtttatctc aacgtcggta acttcctgct cggcgtggcg gcgctgggtc tggacgcggt
     1741 gcccatcgaa ggttttgacg ccgcaatcct cgatgcagaa tttggtctga aagagaaagg
     1801 ctacaccagt ctggtggttg ttccggtagg tcatcacagc gttgaagatt ttaacgctac
     1861 gctgccgaaa tctcgtctgc cgcaaaacac caccttaacc gaagtgtaat tctctcttgc
     1921 cgggcatctg cccggctatt tcccctcaga ttctcctgat ttgcataacc ctgtttcagc
     1981 cgtcatcata ggctgctgtt gtataaagga gacgttatgc aggatttaat atcccaggtt
     2041 gaagatttag cgggtattga gatcgatcac accacctcga tggtgatgat tttcggtatt
     2101 atttttctga ccgccgtcgt ggtgcatatt attttgcatt gggtggtact gcggaccttc
     2161 gaaaaacgtg ccatcgccag ttcacggctt tggttgcaaa tcattaccca gaataaactc
     2221 ttccaccgtt tagcttttac cctgcagggg attatcgtca atattcaggc ggtattctgg
     2281 ctgcaaaaag gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt gtggatcatg
     2341 atgtatgcgc tgctttcagt cttctcgttg ctggatgtta ttttgaatct ggcgcagaaa
     2401 ttcccggcgg catctcagtt accgctgaaa gggatatttc aggggattaa actgatcggc
     2461 gcaatcctgg tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc agcgattcta
     2521 atcagcggtc ttggtgcaat ggctgccgtg ctgatgttgg tatttaaaga tccgattctt
     2581 ggtctggtgg caggtattca gctttccgcg aacgatatgc tgaaactggg cgactggctg
     2641 gaaatgccga aatacggcgc ggatggcgcg gtgatcgata ttgggttaac caccgtcaaa
     2701 gtgcgtaact gggacaatac cattaccact attcccacct ggtctctggt ttctgactcc
     2761 tttaaaaact ggagcgggat gtcagcatct ggcgggcgac gtattaagcg cagtatcagt
     2821 attgatgtca ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg
     2881 catttgttaa agccttattt aaccagccgc catcaggaaa ttaatgagtg gaatcgccag
     2941 caaggttcta cggagtcggt attaaacctg cgccgaatga ccaatattgg aacctttcgt
     3001 gcctatctga acgaatatct gcgtaaccat ccgcggattc gtaaagatat gaccttaatg
     3061 gtacgccaac tggctccagg tgataacggt ttaccgctcg agatctacgc gtttactaac
     3121 accgtggtgt ggctggaata tgaaagcatt caggctgata tattcgatca catatttgcg
     3181 attgtcgaag agtttggtct gcgacttcat cagtcgccaa ccggcaatga tattcgctct
     3241 ctggcgggtg catttaagca gtaattaaaa aaaccgctct catcgaatgg atgagagcgg
     3301 acatcgtttt gtcggatgta gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca
     3361 caaacaggaa tacaatccac accggcagca ggatcgctga caagcgcata tcatccatcg
     3421 tgcacatcag cagcaaaatc atgccgagga aggcgatgca aagatagttg ccgaacggat
     3481 agagcagcgc cttaaactgt gtttcacgcc cctgacgtcg catcgccgcg cgaaaacgca
     3541 gatgcgccag gcagatcata atccagttca acagcaacgt tgctaccacc agcgccatca
     3601 gcagaccaaa cgctttttgc ggcagcagat agttgattaa caccaccagc gaagtgatcg
     3661 ctccggaaag catcagcgag ttaatcggca caccgcgacg gctgacgcga gtcaaaaact
     3721 tcggcgcatt accctgcaca gaaaggccaa acagcatgcg gctgttagag taaaccccgc
     3781 tgttatacac cgacagcgat gccactagaa tgacgaagtt cagcgcagaa gctaccacat
     3841 tgctgtcgag attatggaaa atcatcacaa acgggctact gttggatttc acttccaccc
     3901 acggatagag tgccagtaaa accaccagtg aaccgatata aaacagcagg atgcgataca
     3961 ccacctgatt taccgctttt ggaatgcttt tttccggatc gcgcgcttca gcggctgtaa
     4021 tcccaatcag ctccagaccg ccgaaggaga acataattac cgccagcgac aaaatcagcc
     4081 cattccagcc ggtggcgaag aaaccaccgt agcgccagag gttgtcgata ctggctttct
     4141 cgccgccgta accagaaaac agcagccaca ggccaaagcc gatcataccg atgatcgcca
     4201 gcactttaat cagcgcaaac cagaactcgg tttcgccata taagcgcacg ttcaccaggt
     4261 taacggcgtt gatgataata aagaaggcgg cagcccaaat ccacgttgga acatccggga
     4321 accagtactg catatagatg cccgcagcgg tcagctctgc cattcccacc agcacgaaca
     4381 ttacccagta gttccagcca gagaggaagc ccgcaaacgg tccccagtat ttataggcaa
     4441 agtgggcaaa tgaaccggat accggctctt cgaccaccat ctcgccaagc tggcgcataa
     4501 tcaggaaagc gatgatcccg gcgacgccgt agcccagcaa tacagccgga cccgccatct
     4561 gaatcgccgg gccaatgcca agaaacagac cagtaccaat tgcgccaccc agcgcaatca
     4621 gttgaatatg acggttatgt aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat
     4681 cttccgatac ggttgacgcg tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg
     4741 gcacctttta acatttagtg cccatcgtcg caagacacaa tccacacggt taaacgaggt
     4801 atcctgcttt aattccgtac ccgatgtcgg tgcagccaca tcagcttata cgccgccggg
     4861 ataataaaca gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg tgcggcaatc
     4921 cggctcatca cctctgaacc agcccccgtt ccccacagaa tcggcagcag accggcgata
     4981 atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagga ccgcgccgtg atacaacgcc
     5041 tcatccagct tctgctcgct gaatgtttgt ggattattca acgacggctc ggcctctatg
     5101 gcgtgacgta aatacatcag catcaccacg ccaaattcgg cggcgacccc ggcaagggca
     5161 ataaagccgg tacccgtcgc cacagaaaga tgaaagccca tccaccacag taaccagatg
     5221 ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc cacccgacgg
     5281 aatgccagat acaacagcac gaagatgatc atcagt
//
LOCUS       DAJTYM010000171         5168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTYM010000171 DAJTYM010000000
VERSION     DAJTYM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1584)
                     /locus_tag="OO575_003799"
     CDS             complement(<1..1584)
                     /locus_tag="OO575_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6666578.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLE"
     gene            complement(1600..2484)
                     /locus_tag="OO575_003800"
     CDS             complement(1600..2484)
                     /locus_tag="OO575_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6666579.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2495..3100)
                     /locus_tag="OO575_003801"
     CDS             complement(2495..3100)
                     /locus_tag="OO575_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6666580.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3271..>5168)
                     /locus_tag="OO575_003802"
     CDS             complement(3271..>5168)
                     /locus_tag="OO575_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCR6666581.1"
                     /translation="LVFIDSYYDNPSTIKGSINARGVFINDIIAPVVASSTNSEFMVR
                     ASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIFKRSNIRMAGNVCNQMKKEALF
                     RPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMRTLPRLKGISANLRKQLINSND
                     EQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLN
                     LALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQ
                     LTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRMTPQESDKVAAYLYQKFENDDD
                     LIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSN
                     LAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETSADMILSELISHGEVDDQMLLN
                     AAALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPRNDIWAKFFLRQENSSRAQVDE
                     ALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLTFFKFGALSNRHTPSAALAAEI
                     DPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPELDLVRQLALNGKTRAIREQAM
                     RKLDELY"
BASE COUNT         1487 a         1291 c         1084 g         1306 t
ORIGIN      
        1 ctccagttgt gggtgccacg gctgcccggt gtagatgaga atgccacccg gttcgatggc
       61 attggctaga cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatatag
      121 gccagagaca atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa
      181 cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatctcttg
      241 cccctgtgca acatttaact cactgtaatc acgtaacaaa atatcgctta cggcaggctc
      301 gtttgccagc gcatccagta catagcgtcc atgccctgcg gcaatgtcaa ccacgcggac
      361 ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcaatattt gcaaatgggt
      421 tttgcgctgg cgaataccgc gccatccaac actgttcagg tagattttgt cgattaaacg
      481 cccgaatgcg ttactgcctt gcggttgatt ttgatagaca taatccaggg aactgcctga
      541 atcaaagccg gtactcattc cgagatgcag gcccgcagag tgcgcaccga acaatttcat
      601 cgcctttcgc ataaagcgat acgctaaatc aaccggcgat aatggcacgg gtccaccaga
      661 aaggagccgc catcgatccg ctgatggtcc tgtgcggtct tcatgctgat aatcaaattt
      721 ttgcgatttg ttagcatata agcggcagat aaagctttgc attttttcaa atgcctgcgc
      781 cctgttctct tcacccaatg tgtcgtgata aaagcctggc agcagatgca gctctttcag
      841 agggctacgt aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc
      901 acctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc
      961 agacgtttta tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc
     1021 attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgccctt tcacatagga
     1081 attaataaaa aatagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg
     1141 cacatacaat ttaaccttaa aggccggaga agccagcacc agcccacgaa ttgcaggtgc
     1201 ataatcatga acccatgtgg caacaagcac tgcgccgacg ctttgcgcga tcactaccac
     1261 ctcttccagt ccgacctggc tgtcgctggc agcaaaacgg acaaattcat cgacatcctg
     1321 cactgagcgc ataagagacg ggctataacc acgcggcccc gaattttttc catgcccccg
     1381 ggcatcccag gcataaaaag cagtatcagg catcgccagt tcatcaacga gatgttgtag
     1441 acgaccagaa tgttcatgcc cgcgatgaaa taagacgatg acctttttcg cccctggctg
     1501 taaagtgggc cagtgccgat aatacaatgc tgtattatca ctggtggtaa aaaaatgttc
     1561 cccagggatg cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat
     1621 aaaataaaaa aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc
     1681 atgtcctagt aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat
     1741 cactacatcg ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa
     1801 tgtatttagc ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgattac
     1861 gccccccagc agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct
     1921 gccgcaggat ttcccccata aatactgtgc aatatcgttt gactcggtta acgccaaaag
     1981 aaataacacc agtaacgccc cgccctggat atttatggtt ggcaacatta atagccaggc
     2041 ggcatgactc aaggcaaaaa cggtcattat ccatccccaa aaaatggcgc tcacggtatg
     2101 ctgaaaaccg gaggggtcac ccacaaaaac tcgccaggtg gctaatatca gaaacccagt
     2161 caaaggtata aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag
     2221 aggaataacc caatataacc aacgcgaaaa atgtacggat ataagcgtac aatattcttt
     2281 caatgccaga aaacttatca gcgtaaaaaa cgtcaacgtc atccagcgtg ggcctgaaat
     2341 caccagtgaa aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacttcatc
     2401 cccttttctc tcaggtaacc gccatagcag aaaacggttt atcagcttgc taaaattaac
     2461 aacgcgaaaa gtgttgccag agatttttcc agcattatct ttcagcgctc ataagaagca
     2521 cactccgaca acgattaatc gcagtacaga taagcagaat tgaagcaatg ctccataaaa
     2581 gattattcca ttgcatccac tgtggataaa tggcaacggc cagaccccac agaccaaata
     2641 ttaacgcgcg atcacttttg ccaaatggac cggcataact gcgaacaccg ttaatcgtct
     2701 gcgcgagtaa accgcaaaac tcggtcaata tggtgcaaaa taacatgagt atcacgagtg
     2761 atgcgttact ttccggtaaa aataaaaacg gtaaatagag cgcaatatcg gaaataacat
     2821 cgccagtctc attcaaaatc gcccccagac gtgtttgctg attgcactca cgcgccaaca
     2881 tgccatccag cgcattgagc gccatacgga taaaaagcac gatgggcaat agcaaaaaga
     2941 ggatgggttg tgccaccaac accaacagca atccggtaaa aagagaaagc gccagtgccg
     3001 taagagtgat gtgattcgct gtaacgtggt gcttataaag ccaaaacatc gtcggcctta
     3061 acagcgactg aaagagcggt tttatctggt agagcgtcat tatcaatcct tgataattcg
     3121 gtttgcgcta tggtgcatca attcccctga cggagatatc gcaacacgcc gcttcattaa
     3181 aaattcagaa tatatcaagg tattgggggc tcactgtcag gaatgattat ccccaataaa
     3241 tcaagtagtt aaaaattatt acgctgttat tcaatacaac tcatcaagtt tcctcatcgc
     3301 ttgttcccgt attgcgcgcg tcttaccgtt aagcgccagc tggcggacta aatccagttc
     3361 agggttaaca agctctaacg ccagcaaagg atcacgtttc agcctcgcct tcaatacatc
     3421 aactggaaag cggggattat tcatcgccac aatccaccac tcgggatcaa tttctgccgc
     3481 cagtgccgca gagggggtgt ggcggttgct aagtgcgccg aacttgaaaa atgtgagatt
     3541 gcttttcgcc agtgtactcc accatatacg atccggttgt tttgccagta catccagttg
     3601 tgctaacgca tcggggtcga gtgcataata aacacgtaat gcttcatcaa cctgcgcacg
     3661 ggagctattt tcctgcctca gaaaaaactt cgcccaaata tcattgcgtg gcgtgttttg
     3721 ctgtaattcc ttgagaacaa cagcgggtaa attatcccaa ctaactaatg cactctctaa
     3781 aaagtcccag tcttccaaac gtatcagggc ggcggcattt aatagcatct ggtcatcaac
     3841 ttcgccgtgg ctaatcagct cagacaaaat catatcagcg ctggtttcat atgcatctga
     3901 atctgctttc ggattatttt tccgatgcga taaagcacgt tgccaaattt tttgtttcat
     3961 cgacgtgctc agatactgat ttttcgccag attactccac ccctcaggat cgttgaaacg
     4021 tgtcaataac cgttgtaaag cagcgtcaga gtgaattacc tgcatatccc gacagcaaaa
     4081 gtaggcaggg gattttttct ccatcctttt aacaaaatta aactgtaagt tatctggcaa
     4141 tgctaaaaat aacacacgta ttagatcatc gtcattctcg aatttttggt ataaataggc
     4201 tgcaactttg tcactctctt gtggcgtcat ccgcaataat tgaccatggt gtttcatctc
     4261 tgccagcttg aacgctaagc ttgtgacaac attgtttgat gaatcgttcg ccaactgcat
     4321 tgcctgattg taagtgagtt gtcctccagc accgacctga cgaatagagg aatcatcact
     4381 gcggatatag ccatcaataa tggattcggg caataaccac gcatctttat aaagctcctg
     4441 aatgatcata tcccaatctc ttttcgccag agtcatgacg atatccagag ggagattttc
     4501 acgtagcgca agattcaacg gaccgacacc atcaggtaga cgactcattc gcgtcattat
     4561 ttcgacagag agcggttcac ggtcgagcaa ccccagaaca acaggctggt aacccaattg
     4621 ctgatcggtc aactccagaa tctcattgtc aggcatatat ctcgccatac tttcaatcgt
     4681 ttgttcatca ttgctattaa taagttgttt gcgtaggtta gctgaaattc ctttaagcct
     4741 tgggagagtt cgcattatgg caagatcaaa tgtcgcaata tctcgtcctt caaatgcagc
     4801 aacgttgcct tcatccagca tttgcaattc ctgcactaac tcaggagaag gcttagggcg
     4861 aaacagcgct tcttttttca tctgattaca gacattccct gccatgcgaa tattgcttcg
     4921 cttaaaaatt tctttcagag catcttcatc atttattaga ccccaataat atgcatcagg
     4981 atttattatc atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac
     5041 catgaattca ctatttgtcg aagacgcaac aacaggtgca attatgtcat tgataaatac
     5101 cccacgcgca ttaatactcc ccttaatcgt agacggatta tcgtaatatg agtcgataaa
     5161 taccagtc
//
LOCUS       DAJTYM010000172         5063 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTYM010000172 DAJTYM010000000
VERSION     DAJTYM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..153)
                     /locus_tag="OO575_003803"
     CDS             complement(<1..153)
                     /locus_tag="OO575_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416029.1"
                     /note="regulates the transcription of lsr genes; involved
                     in autoinducer 2 uptake, biofilm formation and stress
                     responses; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6666582.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLL"
     gene            402..1937
                     /gene="lsrA"
                     /locus_tag="OO575_003804"
     CDS             402..1937
                     /gene="lsrA"
                     /locus_tag="OO575_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR6666583.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDHATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            1931..2959
                     /gene="lsrC"
                     /locus_tag="OO575_003805"
     CDS             1931..2959
                     /gene="lsrC"
                     /locus_tag="OO575_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR6666584.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            2959..3951
                     /gene="lsrD"
                     /locus_tag="OO575_003806"
     CDS             2959..3951
                     /gene="lsrD"
                     /locus_tag="OO575_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR6666585.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            3963..4986
                     /gene="lsrB"
                     /locus_tag="OO575_003807"
                     /pseudo
     CDS             3963..4986
                     /gene="lsrB"
                     /locus_tag="OO575_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1151 a         1229 c         1378 g         1305 t
ORIGIN      
        1 cagcaagcgc gacactttca aacgggtcag gccgagacga tcgctgatct cgctctgggt
       61 cagcccgtcg tgatagtaaa accacgcgat ccgcgcgacc tgttcttctt cacacattcc
      121 ctgttctgaa attgccgaat cgttgattgt cataattcat tcttcacttt gaacatattt
      181 aaatctttaa tgcaattgtt cagttcttgc gcatttatat ctgtgatggc aaccacagtt
      241 tgactctacg agcatgaaca aacgcaaccg tgaaaatcaa aatagcataa attgtgatct
      301 attcgtcgga aatatgtgca atgtccacct aaggttatga acaaattaaa agtagaaata
      361 catttgttca aaactcacct gcaaaactga acgggggaaa tatgcaaacg agtgataccc
      421 gcgcgttacc gctactttgc gcccgctcgg tttataaaca gtattcaggg gtcaatgtcc
      481 tgaaaggcat cgattttacg ttgcatcagg gggaggtcca cgccctgctc ggcggcaatg
      541 gtgccggtaa atcgacgtta atgaagatta ttgccggtat tacccctgct gatagcggta
      601 cgctggagat tgggggcaac aactacgcca gattaacgcc agttcatgct catcagctgg
      661 gcatttatct cgttccccag gaaccgctgc ttttcccaag cctgtcgata aaagaaaaca
      721 tcctgtttgg gctggcaaaa aaacagctct ccatgcagaa aatgaagaac ttgctggcgg
      781 cgctgggctg ccagtttgat ctgcatagtc tggcaggatc gctggatgtc gccgatcgcc
      841 aaatggtgga aatcctccgc gggctgatgc gcgactcgcg gattctgatc ctcgatgaac
      901 ctaccgcctc gcttacccct gcggaaaccg aacgcttgtt tagtcgcttg caagagctgc
      961 ttgctactgg cgtgggtatt gtttttatct cgcataagct gccggaaatt cgccagattg
     1021 ccgatcgaat tagcgtgatg cgcgacggaa ccatcgcctt aagcggcaaa accagcgaac
     1081 tgtctaccga cgacattatt caggccatca ccccagcggt acgggaaaaa tcgctctctg
     1141 ccagccaaaa attatggctg gagttacctg gtaaccgccc acaacatgcc gtcggaacgc
     1201 cggtgctgac actggaaaat ctgactggcg aaggcttcag gaatgtcagc ctgacgctca
     1261 atgccggaga aattctgggc ctggctgggc tggtgggagc cggacgcaca gaactggccg
     1321 agacgctcta tggtctgcgt actttgcgtg gcggacgcat tatgctgaat ggtaaagaga
     1381 tcaataaatt atccactgga gaacgtttac tgcgcggtct ggtttatctg ccggaagatc
     1441 gccagtcatc cggactgaat ctcgatgctt cgctggcctg gaacgtctgc gcccttactc
     1501 ataaccttcg tggattctgg gcgaaaaccg cgaaagatca tgccaccctg gaacgttatc
     1561 gtcgggcgct gaatattaaa tttaaccaac cggaacaagc tgcacggaca ttatccggta
     1621 gcaaccagca aaaaatcctc attgccaaat gcctggaagc ttcgccgcaa gcattgattg
     1681 tcgatgagcc gacgcgcggc gtggatgtct cggcccgtaa tgatatctac cagctgttgc
     1741 gcagcatcgc cgcacagaat gtggctgtgc tgtttatctc ctctgatatg gaagagatcg
     1801 aactgatggc agatcgtgtg tatgtgatgc atcagggcga aattacccac tctgcactga
     1861 ccgggcgcga tattaatgtc gagactatta tgcgcgttgc cttcggcgat agtcagcgtc
     1921 aggaggcatc atgctgaagt ttattcagaa caaccgtgaa atcacggcat tgctggcggt
     1981 ggtgctgctg tttgtattac ccggttttct cgaccgccag tatttaagtg tgcaaacgct
     2041 gaccatggtt tatagcagcg cgcaaatcct gatcctgctg gcaatgggcg cgacgctggt
     2101 aatgcttacg cgcaatattg atgtttcagt gggttcgatt accggaatgt gcgcggtgct
     2161 gttggggatg ttactgaacg caggatattc actacctgtt gcctgtgtcg cgactttact
     2221 gcttggtttg ctcgcgggat ttttcaacgg tgccctggtc gcgtggctaa agatccctgc
     2281 cattgttgcc acccttggca cgttagggtt gtacagaggc atcatgttgc tgtggactgg
     2341 cggtaaatgg attgaagggt tacccgccga actgaaacag ctctccgccc cgctgctgct
     2401 tggcatttca gcaattggtt ggttgacgat aattctggtg gcatttatgg cctggctgct
     2461 ggcgaagacg gcgtttggac gcagttttta tgccacgggc gataatttac agggcgctcg
     2521 tcaactgggc gttcgtacgg aagccattcg cattgtggca ttttcgttga acggctgcat
     2581 ggcggcactg gcgggaattg tgtttgcttc gcagattggt tttatcctca accagaccgg
     2641 taccgggctg gagatgaaag caattgcagc ctgcgtgctg ggcggcatta gtttgctcgg
     2701 tggttccggt gcgatcatcg gtgcggtact cggcgcatgg ttcctgacgc agatcgatag
     2761 cgtactggtg ctgttgcgca ttccggcatg gtggaatgat tttatcgcgg gtctggttct
     2821 gctggcggtg ctggtgtttg atggacgcct gcgttgtgcg ctggaacgta atctacggcg
     2881 gcaaaaatat gcccgcttta tgacgccacc gccatccgtt aaacccgctt cgtcaggtaa
     2941 aaaacgggag gccgcataat gcgtattcgc tacggttggg aactggctct tgccgcactg
     3001 ctcgttattg agattgtcgc atttggtgca attaacccgc gaatgttaga tctcaatatg
     3061 ttgctgttca gcaccagtga ctttatctgc attggcattg tcgctctacc gctgacgatg
     3121 gtgattgtca gtggcgggat cgatatttcg tttggttcga ccatcggcct ctgcgccatt
     3181 gcattgggcg tactgtttca aagtggtgtg ccgatgccgc tggcgatact cctgacctta
     3241 ctgctcggcg cattgtgcgg gctgatcaac gccggattaa ttatctatac caaagttaac
     3301 ccgctggtga ttacgcttgg cacgctgtat ctgtttgccg gaagcgctct gctgctttcc
     3361 ggtatggccg gagcgacggg gtacgaaggt attggtggat tcccgatggc gtttacagat
     3421 ttcgctaacc tggatgtgct gggactcccc gttccgctga ttatcttcct gatatgtctc
     3481 ctcgttttct ggctctggct gcataaaacc catgccggac gtaatgtgtt tttgattggg
     3541 caaagcccgc gcgtggcgct ttatagcgcg attccagtta accgcacctt atgtgcgctc
     3601 tatgccatga cggggctggc gtctgcggtc gccgctgtgc tgctggtatc gtattttggt
     3661 tcagcacgtt ccgatctcgg tgcgtcgttt ctgatgcccg ccatcaccgc cgtggtgctt
     3721 ggcggggcca atatttatgg tggttccggt tccattatcg gcaccgccat tgcggtttta
     3781 ttagtgggat atttgcagca aggtttgcaa atggcaggag tgccaaatca ggtgtccagc
     3841 gccctttccg gtgcgctgct tatcgtcgtt gtcgtaggtc gttccgttag cctgcatcgc
     3901 cagcaaatta aagagtggct ggcgcgtcgg gccaataacc cattgccata aaggatatct
     3961 tcatgacact tcatcgcttt aagaaaatcg ccttacttag cgctcttggc attgccgcaa
     4021 tctctatgaa tgtgcaggcc gcagagcgta ttgcatttat tcccaaactg gttggcgtgg
     4081 gattttttac cagcggtggc aacggcgcac aacaagcggg taaagagctg agcgttgatg
     4141 tgacctacga cgggccgaca gaacccagtg tttctggtca ggtacagttg attaataact
     4201 tcgtcaatca aggttataac gccattattc gtttctgcgg tttcgcctga tggcttgtgt
     4261 ccggcactga aacgcgccat gcaacgtggt gtgagagtgc tgacctggga ctctgatact
     4321 aaaccggagt gccgctctta ctacattaat cagggaacgc ccgcccagtt gggaggtatg
     4381 ttggtggata tggcggcgcg tcaggtgaat aaagacaaag ccaaagtcgc gtttttctac
     4441 tcaagcccca ccgttacgga ccaaaaccag tgggtgaaag aagcgaaagc gaaaatcgcc
     4501 aaagagcatc caggctggga aattgtcact acgcagtttg gctataacga tgccactaaa
     4561 tcgttacaaa ccgcagaagg aatattaaaa gcgtatagcg atctcgacgc cattatcgcc
     4621 cccgatgcca acgccctgcc cgctgccgca caagccgcag aaaacctgaa aaatgacaaa
     4681 gtagcgattg tcggattcag tacgccaaat gtgatgcgcc cgtatgtaga gcgcggcacg
     4741 gtgaaagaat ttggactgtg ggatgtggtt cagcaaggca aaatttcagt gtatgtcgcg
     4801 gatgtattat tgaaaaaagg atcaatgaaa acgggcgaca agctggatat ccagggcgta
     4861 ggtcaggttg aagtctcgcc aaacagcgtt cagggctatg actacgaagc ggatggtaat
     4921 ggcatcgtac tgttaccgga gcgcgtgata ttcaacaaag agaatatcgg caaatacgat
     4981 ttctgatgtg cattacttaa ccggagtaag ttatggcaga tttagacgat attaaagatg
     5041 gtaaagattt tcgtaccgat caa
//
LOCUS       DAJTYM010000173         5003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTYM010000173 DAJTYM010000000
VERSION     DAJTYM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            38..112
                     /gene="tnaC"
                     /locus_tag="OO575_003808"
     CDS             38..112
                     /gene="tnaC"
                     /locus_tag="OO575_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6666586.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            333..1748
                     /gene="tnaA"
                     /locus_tag="OO575_003809"
     CDS             333..1748
                     /gene="tnaA"
                     /locus_tag="OO575_003809"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6666587.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1839..3086
                     /gene="tnaB"
                     /locus_tag="OO575_003810"
     CDS             1839..3086
                     /gene="tnaB"
                     /locus_tag="OO575_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6666588.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3218..4393
                     /gene="mdtL"
                     /locus_tag="OO575_003811"
     CDS             3218..4393
                     /gene="mdtL"
                     /locus_tag="OO575_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6666589.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4368..>5003
                     /gene="yidZ"
                     /locus_tag="OO575_003812"
     CDS             4368..>5003
                     /gene="yidZ"
                     /locus_tag="OO575_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6666590.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQ"
BASE COUNT         1139 a         1203 c         1250 g         1411 t
ORIGIN      
        1 ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac atgtatgtgt
       61 gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt aatttaccct
      121 tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta gtttgctata
      181 tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt agtaaatgat
      241 ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat tcacagggat
      301 cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat ctccctgaac
      361 cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat cgtgaagaag
      421 caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg tttatcgatt
      481 tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg atgatgcgcg
      541 gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca gtgaaaaata
      601 tctttggtta tcaatatact attccgactc accagggccg tggcgcagag caaatctata
      661 ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc aaaatggtgg
      721 cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac ggctgtaccg
      781 tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac tttaaaggca
      841 actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat aacgtgccgt
      901 atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca ctggcaaact
      961 taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg gactccgcac
     1021 gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa gactggacca
     1081 tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg tccgccaaga
     1141 aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc ttctttgatg
     1201 tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca tatggcggct
     1261 tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg aatctcgact
     1321 ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa gagattggcg
     1381 ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa ctgctgccgc
     1441 atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg
     1501 gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa accggtaaac
     1561 aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa
     1621 cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg gcgaatatta
     1681 aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca aaactgaaag
     1741 aagtttaatt aatactacag agtggctata aggatgttag ccactctctt accctacatc
     1801 ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa
     1861 agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt ggaggtatgt
     1921 ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt atccttatca
     1981 ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat ttaaattatc
     2041 ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc tggaacatta
     2101 tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat atctctgcta
     2161 atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct aatccacgta
     2221 ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt tcgttagccg
     2281 ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg
     2341 gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc accactgcgg
     2401 gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg tcatttggtt
     2461 tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat aagttaatca
     2521 aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg ctctattgca
     2581 cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc ggcaacattg
     2641 attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag ttttgcctgc
     2701 tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg gggttgttcg
     2761 attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc aaaaccgtgc
     2821 tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc tttatttacg
     2881 ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc gcagtgcttg
     2941 caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc ggcaatctta
     3001 ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc ggcaacgtct
     3061 ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg ctggcttctt
     3121 gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct tcaccacgta
     3181 tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt tgatttgtag
     3241 ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg gtttaccgcg
     3301 catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct ccgtatatct
     3361 ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt cagggagaaa
     3421 gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct gttcactggc
     3481 tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg caggctgttg
     3541 ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc gggctaaagt
     3601 gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag tgctcggaca
     3661 tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa tgatgggcat
     3721 cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc
     3781 ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc tcagccgtgt
     3841 tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat cgccggtatt
     3901 gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg cgctgaccgc
     3961 tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt ttaagccacg
     4021 tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc ttgccgtttc
     4081 accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt tctcggtagg
     4141 ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg gcgtagccag
     4201 ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc tggccgcggt
     4261 ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta gcatagtgag
     4321 cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg aagaaatcca
     4381 tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag gagcgcagcg
     4441 tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag tcgctggcaa
     4501 agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg ctgtcgccca
     4561 cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg agcaatctgc
     4621 tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg gcggcggaat
     4681 cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc
     4741 aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt actcgtggtg
     4801 aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag ctgttaagct
     4861 cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc gtctggttac
     4921 gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta cgttatccgc
     4981 atatcagcat ttgctgggaa cag
//
LOCUS       DAJTYM010000174         4967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTYM010000174 DAJTYM010000000
VERSION     DAJTYM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..530
                     /locus_tag="OO575_003813"
     CDS             <1..530
                     /locus_tag="OO575_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6666591.1"
                     /translation="LYITPVGAAGGVVIKAGEVIARIHMYKIATLGSGNPRNFTWNII
                     SNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQ
                     VFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVS
                     AGTVQSVIGVTFIYE"
     gene            864..3143
                     /gene="ydeP"
                     /locus_tag="OO575_003814"
     CDS             864..3143
                     /gene="ydeP"
                     /locus_tag="OO575_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6666592.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3391..3594
                     /gene="safA"
                     /locus_tag="OO575_003815"
     CDS             3391..3594
                     /gene="safA"
                     /locus_tag="OO575_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6666593.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3662..4423
                     /gene="ydeO"
                     /locus_tag="OO575_003816"
     CDS             3662..4423
                     /gene="ydeO"
                     /locus_tag="OO575_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6666594.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4825..>4967
                     /locus_tag="OO575_003817"
     CDS             4825..>4967
                     /locus_tag="OO575_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6666595.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1420 a         1054 c         1109 g         1384 t
ORIGIN      
        1 aactctatat taccccagtt ggggcggccg gtggagttgt cataaaagcg ggggaagtca
       61 ttgcccgtat tcatatgtat aaaattgcaa cattaggaag cggaaatccg cgtaatttta
      121 cgtggaatat tatttcaaat aacagtgttg ttatgccaac aggcggctgc accgttgata
      181 gtcgtaatgt caccgtggat ctcccggact ttcctggaag cgcagaaata ccccttggcg
      241 tatattgctc aagcgaacaa aagctctcat tttatctttc gggtgccacc actgacagta
      301 gccgacaagt gtttgctaac acagcacctg atgcaacaaa agcatcggga gtgggtgtca
      361 ctctaatgcg taatggaaaa atactggcta cgggagaaaa tgtctccctg gggacagtaa
      421 ataaaagcaa agtacctctg ggtttaagcg caacctatgg acaaacagga aacaaggttt
      481 cagccggaac cgtccagtca gtcattggtg tcacttttat ctatgaataa ttgtgatttt
      541 gaccttagtc tatgcgctcc attcacgaaa attgcgcgga taattatttt gtgaaggcta
      601 ttatcctaca cctgtaagaa aatccgcgca caattgaagc cagagtctta ttttttatat
      661 tggaaaatat gttaatttaa acactatgag agatgatgct caacttatcg tatttaatct
      721 attgtttaac gataatagat taaaaataga aagagagtag tcccttactc ttatatatcc
      781 atgttggcga taatcccttt gtatgtactg tgcatcatcg ctattacaaa tcctaataat
      841 tcatttccac acaggataag tagatgaaga aaaaaattga atcctaccag ggtgctgcag
      901 gtggttgggg tgctgttaaa tccgtagcga atgcagtacg taagcagatg gatatacgcc
      961 atgatgttat tgccatgttt gacatgaata agccagaggg ctttgactgt ccgggttgtg
     1021 catggccaga tcctaagaac agtgcgtcat tcgacatttg tgaaaacggc gcaaaagcaa
     1081 tcgcttggga agtcacggat aagcaggtaa atgcctcttt ctttgctgag aatacggttc
     1141 aatcattact tacctgggga gatcacgagc ttgaggctgc ggggcgtctc actcagcctt
     1201 tgaaatatga tgccgtcagc gactgttaca agccattaag ctggcaacaa gctttcgacg
     1261 aaattggcgc acgccttcaa agctatagtg atcccaatca ggttgaattc tatacttcgg
     1321 gccgcacttc caatgaagct gcctttcttt atcagctttt tgcccgtgaa tacgggagca
     1381 ataactttcc cgactgctcc aacatgtgcc atgaaccgac aagcgtgggt ttggcagcga
     1441 gtatcggtgt aggtaaaggg accgtgttgc tggaagactt tgagaagtgc gatttagtca
     1501 tttgcattgg gcataaccct ggtacaaacc accctcgcat gctgacttcg ttgcgcgctt
     1561 tagtgaaacg gggagcgaaa atgatcgcca tcaatcctct acaggaacgt ggcctggagc
     1621 gatttaccgc accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact cagttggcca
     1681 gtgcctacta caacgtgcgc attggtggcg atatggcgtt gctcaagggg atgatgcgcc
     1741 tgttaattga gcgcgatgat gctgcaagtg ccgcaggtcg gccctcattg cttgatgacg
     1801 aatttattca aacgcatacc gtcggctttg acgagctacg ccgtgacgtt ctcaattccg
     1861 agtggaaaga tatcgaacgt atttctggac taagtcagac acaaatcgcc gaactggctg
     1921 acgcatatgc cgctgccgaa cgcaccatta tctgttacgg aatggggatc actcagcacg
     1981 aacatggtac ccagaacgtg cagcaactgg tcaatttgct gttgatgaaa ggtaacattg
     2041 gcaagcctgg tgcgggtatc tgcccactac gtggtcactc taatgtacag ggcgaccgaa
     2101 ccgtcggtat caccgagaaa ccgtctgcag agtttctggc tcgcctgggt gagcgctatg
     2161 gcttcccccc acctcatgca cctggacatg ctgcaattgc cagcatgcaa gcaatatgta
     2221 cggggcaggc tcgagcattg atctgcatgg ggggcaactt tgcactggca atgccagatc
     2281 gggaagcgag cgctgtaccg ttaacgcaat tagatttggc ggtacacgta gccactaagc
     2341 ttaaccgctc tcatctgctg accgcacggc atagctatat tctgccggtt ctggggcgta
     2401 gcgagattga tatgcaaaaa agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga
     2461 tgattcatgc ctcgcgtggc gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg
     2521 cagtggtcgc gggaatcgcg caggcagcac taccccagag cgtggtagcc tgggagtatc
     2581 tggtggaaga ttatgatcgc attcgcaatg acattgaagc tgtgctgcca gagttcgccg
     2641 actataacca gcgcatccgt catcccggtg gttttcacct gataaatgca gctgctgaaa
     2701 ggcgctggat gacgccgtca ggtaaggcta atttcattac cagcaaaggg ctgttagaag
     2761 atccctcttc agcgtttaac agtaagctgg tcatggcgac agtacgcagc cacgatcagt
     2821 acaacacgac gatttatggt atggatgatc gctatcgagg ggtattcggt caacgagatg
     2881 tggtctttat gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc gaaagagtta
     2941 atcttattgc gcttacgcca gacggtaagc gcagctcacg ccgcatggat agattaaaag
     3001 tggtcattta ccctatggct gaccgctcac tggtgaccta ttttccagaa tcgaatcaca
     3061 tgctaacact tgataaccac gatccattaa gtggtattcc tggctataaa agtattccgg
     3121 ttgaattaga accatcaaat taatgcctct tctcatttct tctgctgtca tccgcacagc
     3181 agaagaattc ctcattgact attattgcgc aatttgctca catggattaa attaaactac
     3241 atactataag atataaactt ctgcctacag ctgtaagaaa ctccgctcag tactgaagca
     3301 ccagtcctat ttcttctttt ctccagcctg ttatattaag catactgatt aacgactttt
     3361 aacgttatcc gctaaataaa catatttgaa atgcatgcga ccacagtgaa aaacaaaatc
     3421 acgcaaagag ataactataa agaaatcatg tctgtaattg tggttgtctt attactgaca
     3481 cttacgttga tagccatttt ttcggcaatt gatcagctga gtatttcaga aatgggtcgc
     3541 attgcaagag atcttacaca tttcattatc aatagtttgc aagactggaa ataaacagca
     3601 aattataaat gtgaaatgtt taaaaggtat cgataaaact ttattgtttt aaggagataa
     3661 aatgtcgctc gtttgttctg ttatatttat tcatcatgct ttcaacgcta acattttaga
     3721 taaagattac gccttctccg acggcgagat cctgatggta gataacgctg ttcgtacaca
     3781 ttttgaacct tatgagcggc attttaaaga aatcggattt aatgaaaata ccattaaaaa
     3841 atatctacaa tgcactaaca tccagacagt gacggtgcct gttcctgcga agtttttacg
     3901 tgcttcaaat gtaccgactg gattgcttaa tgaaatgatt gcttatctca actcggaaga
     3961 acgcaatcat cataattttt cagaactttt gcttttttct tgcctgtcta tttttgccgc
     4021 atgcaaaggt ttcattacac tattaactaa cggtgtgcta tccgtttccg ggaaagtgag
     4081 aaatattgtc aacatgaagc tggcgcaccc atggaagctg aaagatattt gtgactgcct
     4141 gtacatcagt gaaagcctgt tgaagaaaaa actaaagcaa gagcaaacga cattctcaca
     4201 gattctatta gatgccagaa tgcagcacgc aaaaaatttg atacgcgtag aaggttcagt
     4261 caataaaatt gccgaacaat gtggttatgc cagtacatct tattttattt atgcgttccg
     4321 caaacatttc ggcaacagtc cgaagagagt ttctaaggag taccgttgtc aaaggcacac
     4381 gggtatgaat acgggcaaca cgatgagtgc tttagctatt tgattatttg ctaacgagta
     4441 gtcaaccacg cacgctacgt aagaattata tggggcagcc attccctgcc ccgcgttgtt
     4501 tttaggcgat atatttattg aaataaataa gtgacatcca tcacatattt atgcacttgc
     4561 ataacctgtt gcatgattat ttatgatctc aattcggcat tttgtcagta aaatgcaata
     4621 atttattaaa tatcaataaa ttagttgttt atcggcgaga aattacttaa tagaacagaa
     4681 agtaatgtca acgctttatg gactgttatt tccctttttt tagctaaatc tgctatctct
     4741 ttatgtgact aacttcactt acatccactt atttctcttc gtaaaattac ttcggaatta
     4801 agtacaataa gaagaggaac atttatgaag tctgcattaa agaaaagtgt cgtaagtacc
     4861 tcgatatctt tgatactggc atctggtatg gctgcatttg ctgctcatgc ggcagatgat
     4921 gtaaagctga aagcaaccaa aacaaacgtt gctttctcag actttac
//
LOCUS       DAJTYM010000175         4947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTYM010000175 DAJTYM010000000
VERSION     DAJTYM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..800)
                     /locus_tag="OO575_003818"
     CDS             complement(<1..800)
                     /locus_tag="OO575_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR6666596.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDK"
     gene            complement(812..1301)
                     /locus_tag="OO575_003819"
                     /pseudo
     CDS             complement(812..1301)
                     /locus_tag="OO575_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1305..2525)
                     /locus_tag="OO575_003820"
     CDS             complement(1305..2525)
                     /locus_tag="OO575_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6666597.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2529..3272)
                     /locus_tag="OO575_003821"
     CDS             complement(2529..3272)
                     /locus_tag="OO575_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6666598.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3163..4629)
                     /locus_tag="OO575_003822"
     CDS             complement(3163..4629)
                     /locus_tag="OO575_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR6666599.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4629..4862)
                     /locus_tag="OO575_003823"
     CDS             complement(4629..4862)
                     /locus_tag="OO575_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR6666600.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
BASE COUNT         1051 a         1395 c         1245 g         1256 t
ORIGIN      
        1 tttttatcgt tggtgtacgc aactatgcga tccttattcc ccacaccacg gcctttcagc
       61 catttaaccg cgcggatatt cagcggaaca ccgttctggt gatagctgat ggtgttggtc
      121 tgaaggttcg tcaacaggga ctggttacct gcagatgaaa cgatgatgct ggacaacaga
      181 gcaaactgct caggcgggat cagcaaatca cgcggaacca cagagtaacc agaagcggcc
      241 cacgcatcag acagcacctg gttaatgctt gcgcggattt cgtccggtgt tgaggttgcc
      301 cacgttttgg cagcgttgtt gacaggcacg ccgtccaggg taacaaggcc tttcaggttt
      361 aatgcggaat cgccaacata tacctgttca tcgttatcca tctgccattt cagttgcata
      421 ccgtcatact tctgcgtatc aatcgggcgg ccgacctgct gagcagcctg caattctatg
      481 accgtccagc caagttccat cccccacagg ttcagcgggt taccggattt gccgatatcc
      541 acgttcacgc cagcaatagc ggttgagtct ttgcctaccc agttttttcc attcggattt
      601 gcgccagtac ccgcagcggc gaagctggta ttcgtccagc tggaaatgtc atctgcgatg
      661 gaggcgtctt cacgcagttg aatatcgcgg ctccaggtgt accccaccag tggcaggttc
      721 agcgtctggt cgagtctctc cagctccccg atgagaaagg caccagagct gtcaacggtt
      781 gcctgatcaa aagtaatcat tcgtctgttc cttaaatctt ccaggaaatt tctgcattgc
      841 cgtcagcatc accggcacct gtgaattcag cgttggtcag caccacgttt ttgccactga
      901 ctgacgtgga catgaatcca cccagaggca ctttgatgga ttcatcagtg gagatcgcct
      961 tttttgatag tgctggcatc aaaatcagaa ccgagattaa cggtcacgta gccacgcttc
     1021 atggcgtcgc ccgggaagtt cttgcctgtt cccacctggc gaaccatgtc cggctgcgac
     1081 gtggtcggat aagggcgcac gtagatcccc ttcaccttgt ctgcggtatc accatctgcc
     1141 agcggcacga aaaaaccgtc atcatcgtat ttaccagcca ggccataggc agcgaaggcg
     1201 ttatcggatt taaggaccac cggttcgacg gttaagtcct gcgggcgaga gacagccccg
     1261 gcaatgccaa caggcatccg gtacagaaat acattattca ttttttaccc tttacggttt
     1321 gcccagaatt cagcgttttg tttgttcagg gaagcgatac tggtcatgcc catgtttagg
     1381 cgctgtgcat cgccggtggt ggcgcgggtg tttcgccctt tggcaacctc agacacggca
     1441 ttaaacgcca tgtcgaccga ttgtttcggt aatttgcgga tatccgcatc accgactatc
     1501 tggcgaacca gcgttttgtc agcagaagcc agaacctcgc gtttgaacgc ggtcggtttc
     1561 atcttacggc tcagatcgat acccggaacg ataacttcgg cacgccaggc tgagtcacca
     1621 gtaatcgtgg tttcctcttc atcgtcctcg ccgtcaccgg tcggattatc gtcaggctta
     1681 ttatcgttat cgcccgtggc atttctttcc agcttagcca gcagggcttt cagtaatgtt
     1741 ttgaggtcat catcactgtc gccggttgga tctccgccca tctctggtgc tttgtccggt
     1801 agtggttgct gcggggacag gttgatgttg agattaacgc cctgcggcaa atccccctca
     1861 tctcctgtaa ccgatgcggg agccgactcc accagttcgt tcatggtgtc ggcatctcct
     1921 gtcttgatgg ccgcacgcat gcggttccac cagtttttct tttgatttgc cattgtgtct
     1981 ctgtctccaa ttgcacaacg atttccggct ctgcctttag ggacaagagc cacatggttt
     2041 ccggtaatat cgacctgctc ggctttacct ggctcggtct gctcgtactc cgcgtcatag
     2101 ccgcacgaca cttcgcgcag gccatcttcg ataagctgaa tggcgctttc gtctttgacg
     2161 ataaggtcag ccagcatcaa atcagactgc tcacccgtcc cgcgccggac attctggagg
     2221 tgcccgacag caagcacttt ccagttctcg ggatttacca gccgcacatt cccgttttca
     2281 tcttcaggat gcaggatcgt gatgctcatc ccttcgaatg aggcaagcgt ggccggatgg
     2341 aatacctgct caggagaacg cgtgacgact atttcaacga acttatcggg tttcagtttt
     2401 ggcaggtcat cagcaccata gagctgctta cctgttcgtc ctatcggcac gtctttgcac
     2461 agcaacgagc cgtcagccag gcggtatcgg gtttccccga gcctggtctc aaaaaagtat
     2521 ttcatgggtt atgctgccct tttcattgcg aatggaacgc gaacaaacac gatctcttta
     2581 tagcagcggc agtttggcag ctcgcccgcg tggccggtca tgccgtccaa agttggaggt
     2641 gaatcccagc ggacgaattt acctttcatg tgatcgtggg aatctcggac atcgccatca
     2701 tcggctgtac gccaaatata accatccgag ccaatagcta tcgctcgggc catatccagc
     2761 gcgcccgttg ctcttcccag ttccgttcgg gcaatcagat cggccctaga cttagcaaca
     2821 tcgccggtag atgcaatctc tttagcaaac tgctccgccc gaccaccggt gacaacagcc
     2881 tctatcgctt tgttctggat gtcgtacacc ctgtctgcgg cctcgagagg cagagatttg
     2941 atgtacttga cctgttcggc gatgatggat tgcatcacct ggcccacagg agcgctttcc
     3001 acaagattgc ggagctcgcg actgatgttc ttgctgtgtt gccgccaaac tttctcgttc
     3061 tgccgggtta ggtccgcagt aaagttttcc gcgacctttg tcgcccaggg ggtgatgatt
     3121 tcactgtaac gctccagcgc cgcaataatt tccgtgatac tgtcatttga accatcgtag
     3181 cgaccattta cgatgtctcc gaccgccctc gctatcctgc gtaggctggt tcgataccgg
     3241 atttccgcct gacggctcct gcggttcgtc atcaggttcg ccgatgccgg gcggcgcttc
     3301 atcttcggca ttctcgatgt cctcgttggt aatggatgcc cctatgccgg ttacgtcaga
     3361 attttctcgc aaatcagtca tagcggcttt cagtgtcatc agaccatcac ccagcgccgt
     3421 actgattgcg ttggtggtgt ttaacgccac cgttgagcga tcgacatcag acatttgcca
     3481 gagcgggtta aactcaaacg tgaaatcgtc cggcagcggc ttgccaagtt ccgaacgatg
     3541 catgatgtcc agtatccgcc gcaccggaag acgtaaacgc ctctcctgca acgaactgat
     3601 gcgatcgtaa tagttggcaa ggtctgcatc accggtagaa aatcctttcg gggactgtcc
     3661 gaacaaccgc accagtggga taccaacagc gccactaatc tgttctgcaa actgcgaaag
     3721 gatgtcatcc agaccactga agctgtactg atgggtttca aacttatccc gcgagtccat
     3781 gagcgtcatg ccttcattac tctggaactg tcgaatcagg tcgatattct tcagcaacgc
     3841 ttcatacgca ggaccaccaa gtgcgataag ctcgcgtagc ttctccacgc tgtaggtacg
     3901 caaatgcgcc ttgtagacca gctgcgccgc gccgacagta gcgctgtcga acgcggtaag
     3961 acgatcccag atacgctcta caaccgacat tccccattcg ttctcggtca tcttctgctg
     4021 aaatggcaac gtgacgccat caaagcgaat caggcgactg tgatgaatgc gccaggcagg
     4081 aattcccgtt gctgtggtca ccacatcgta aaactcaggt ttacccaggt ccggccccat
     4141 atctttaatg cggcgggtca gtgccgggtc gaccatccag cggtcgagcg ggagaatccc
     4201 cttaaacttg cccttaccga tggtttcggg tcgcagcggg gtcattggtg cctgcccctc
     4261 aatcatgatg aaacccaccg cgccgccgta gaggcgcgac catttcagca cgtcattcag
     4321 cgcatcccag atttgcaact catccagttg tgattcgaga atgccacgat cttttgcatc
     4381 aatttccgaa gtgatgcgaa tgcctttgcg ggtcatatca tccgggatag catcgactgc
     4441 ttcgccgatg atccaggatg aacgatagga ccattccacc agcatgcggt tacgactggt
     4501 gaaattagcc cggtaggtgg atgctgagtg ctggttaggt gtctgcatcc ctactcgggc
     4561 aataaaattc tcataaccat cagctgtggc ctgcacagtt cgccgcaggg cttgtttgtt
     4621 tcgtgccatc aggcctgtct ccccagctgt tcccagatat ccagcgatgt atcgattggc
     4681 gcgaaggcca taatgaatgc atcagcaacg tttggtgacg gtatctcgcg ttttgcgagg
     4741 tcttttttac tttcgaccat cacacgtccg ttacggtcga aatcacgatg aggtgttgtc
     4801 agttccagtt tcagcttttc aagcaacgga caacgagaat ctatgctgat cagctcatcc
     4861 acaggatact gttctccgtt gttaatggcg ttaaacgtat ttctgaaacg gtcagccacc
     4921 atgcctgagc cttaagattt gcgaaaa
//
LOCUS       DAJTYM010000176         4941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTYM010000176 DAJTYM010000000
VERSION     DAJTYM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            180..437
                     /locus_tag="OO575_003824"
                     /pseudo
     CDS             180..437
                     /locus_tag="OO575_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO575_003825"
     CDS             complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO575_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6666601.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO575_003826"
     CDS             complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO575_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6666602.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO575_003827"
     CDS             complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO575_003827"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6666603.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3062..3205)
                     /locus_tag="OO575_003828"
                     /pseudo
     CDS             complement(3062..3205)
                     /locus_tag="OO575_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3595..4410)
                     /locus_tag="OO575_003829"
     CDS             complement(3595..4410)
                     /locus_tag="OO575_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6666604.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4507..>4941
                     /locus_tag="OO575_003830"
     CDS             4507..>4941
                     /locus_tag="OO575_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6666605.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSA"
BASE COUNT         1313 a         1127 c         1172 g         1329 t
ORIGIN      
        1 cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat tagctaacta
       61 atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag acaacggtga
      121 aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag gattacatca
      181 tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg
      241 ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt atagaacagt
      301 acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca tcgatgattt
      361 ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg
      421 gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa
      481 gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt
      541 aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa
      601 tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg cctgtaaatg
      661 ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc
      721 attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc cacgaatggc
      781 atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac
      841 cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt
      901 ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac
      961 cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct
     1021 tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc
     1081 atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat
     1141 ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag
     1201 ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat
     1261 agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg
     1321 caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc
     1381 tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca
     1441 gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt
     1501 gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga
     1561 cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg ccagcaagct
     1621 gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc agcataccgc
     1681 ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac
     1741 caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa
     1801 aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca
     1861 acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct
     1921 gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga atttcacata
     1981 ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa
     2041 atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga
     2101 ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag
     2161 ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct
     2221 gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct
     2281 ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca
     2341 taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt
     2401 aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc
     2461 tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa caagctggca
     2521 caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg
     2581 ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg
     2641 tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg
     2701 gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg
     2761 tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc
     2821 aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac cacctgggtt
     2881 ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat
     2941 gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac
     3001 ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct
     3061 cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga taccacgcca
     3121 gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact aacgaatagt
     3181 aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga ttttttaatt
     3241 tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga taattccagt
     3301 cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata gccgccagac
     3361 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
     3421 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
     3481 gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt gttataacgg
     3541 ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata taagttaaat
     3601 catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt taccgggcga
     3661 gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat agcccagacg
     3721 atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg
     3781 catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag
     3841 tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg
     3901 gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc
     3961 aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa
     4021 acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag
     4081 taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact
     4141 ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg
     4201 cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc
     4261 agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg
     4321 ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg
     4381 tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca
     4441 aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa
     4501 agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg
     4561 actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat
     4621 gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct
     4681 gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat
     4741 gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc
     4801 aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt
     4861 gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat
     4921 gaaaaactcg cacaatcagc g
//
LOCUS       DAJTYM010000177         4917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTYM010000177 DAJTYM010000000
VERSION     DAJTYM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..943
                     /gene="tauA"
                     /locus_tag="OO575_003831"
     CDS             <1..943
                     /gene="tauA"
                     /locus_tag="OO575_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6666606.1"
                     /translation="TLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGA
                     SVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALV
                     VKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQR
                     GDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFA
                     KSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELT
                     GPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            956..1723
                     /gene="tauB"
                     /locus_tag="OO575_003832"
     CDS             956..1723
                     /gene="tauB"
                     /locus_tag="OO575_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6666607.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1720..2547
                     /gene="tauC"
                     /locus_tag="OO575_003833"
     CDS             1720..2547
                     /gene="tauC"
                     /locus_tag="OO575_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6666608.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2544..3395
                     /gene="tauD"
                     /locus_tag="OO575_003834"
     CDS             2544..3395
                     /gene="tauD"
                     /locus_tag="OO575_003834"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6666609.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3501..4475)
                     /gene="hemB"
                     /locus_tag="OO575_003835"
     CDS             complement(3501..4475)
                     /gene="hemB"
                     /locus_tag="OO575_003835"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6666610.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1112 a         1244 c         1401 g         1160 t
ORIGIN      
        1 cacacttctt gccgcactgg cattcatcgc ttttcaggca caggcggtga acgtcaccgt
       61 ggcgtatcaa acctcagccg aaccggcgaa agtggctcag gccgacaaca cctttgctaa
      121 agagagcggg gcgagcgtgg actggcgtaa gtttgacagc ggagccagca tcgtgcgggc
      181 gctggcttct ggcgacgtgc aaatcggcaa cctcggttcc agcccattag cggttgcagc
      241 cagccaacag gtgccgattg aagtcttctt gctggcgtca aaactgggta actccgaagc
      301 gctggtggta aagaaaacta tcagcaaacc cgaagatttg attggtaaac gcattgccgt
      361 accgtttatc tccaccaccc actacagcct gctggcggcg ctgaaacact gggggattaa
      421 accagggcta gtggagattg tgaacctgca gccgcccgcg attatcgccg catggcagcg
      481 gggtgatatt gatggtgctt atgtctgggc accggcggtt aacgccctgg aaaaagacgg
      541 caaggtgctg accgattctg aacaggtcgg gcagtggggt gcgccaacgc tggacgtctg
      601 ggtggtgcgc aaagattttg ccgagaaaca tcctgaggtc gtgaaagcgt tcgccaaaag
      661 cgccatcgat gcacagcaac cgtacattgc taaccctgac gcgtggctga aacagccgga
      721 aaacatcagc aaactggcgc gtttaagcgg cgtgcctgaa ggtgacgttc cggggctggt
      781 gaagggaaat acctatctga cgccgcagca acaaacggca gaactgaccg gaccggtgaa
      841 caaagcaatc atcgacaccg cgcagttttt gaaagagcag ggcaaggtcc cggctgtagc
      901 gaatgattac agccagtacg ttacctcgcg cttcgtgcaa taaaaggagg cgcagatgct
      961 gcaaatctct catctctatg ccgattatgg cggcaaaccg gcactggaag atatcaacct
     1021 gacgctggaa agcggcgagc tactggtggt gctggggccg tccggctgcg gtaaaaccac
     1081 cctgctgaat ctgattgccg gttttgtgcc ttatcagcat ggcagcattc aactggcggg
     1141 taagcgtatt gagggaccgg gagcagagcg tggcgtagtt tttcagaatg aagggctgct
     1201 accgtggcgc aatgtacagg acaacgtggc gttcggcctg caattggcag gtatagagaa
     1261 aatgcagcga ctggaaatcg cgcaccagat gctgaaaaaa gtggggctgg aaggcgcaga
     1321 aaaacgctat atctggcagc tttccgatgg tcaacgtcag cgggtgggga ttgcccgcgc
     1381 gctggcggcg aatccccagc tgttattact cgacgaaccg tttggtgcgc tggacgcctt
     1441 cacccgcgac cagatgcaaa ctctgctgct gaaactctgg caggagacgg gcaagcaggt
     1501 gctgttgatt acccacgata tagaagaagc ggtgtttatg gcgactgaac tggttctgct
     1561 ttcatccggc cctggccgtg tgctggagcg gctgcggctc aactttgctc gccgctttgt
     1621 tgcgggagag tcgagccgca gcatcaagtc cgatccacaa ttcatcgcca tgcgcgaata
     1681 tgttttaagc cgcgtatttg agcaacggga ggcgttctca tgagtgtgct cattaatgaa
     1741 aaactgcatt cgcggcggct gaaatggcgc tggccgctct cgcgtcaggt gaccttaagc
     1801 attggcacgt tagcggtttt actcactgta tggtggacgg tggcggcgct gcaactgatt
     1861 agcccgctgt ttttgccgcc gccgcaacaa gtactggcaa aactgctcac cattgccgga
     1921 ccgcagggat ttatggacgc cacgctgtgg cagcatctgg cagccagcct gacgcgcatt
     1981 gtgctggcgc tactcgcggc ggtggtgatc ggtattccgg tcgggatcgc catggggcta
     2041 agcccgacgg tgcgcggcat tctggatccg gtaatcgaac tttatcgtcc ggtgccaccg
     2101 ctggcttatt tgccgctgat ggtgatctgg tttggtattg gtgaaacctc gaagatctta
     2161 ctgatctatt tagcgatttt tgcgccggtg gcgatgtcgg cgctggcagg ggtgaaaagc
     2221 gcgcagcagg ttcgcattcg tgccgcccag tcgctgggtg ccagccgtgc gcaggtgctg
     2281 tggtttgtca ttttgcccgg cgcgctgccg gaaatcctca ccggattacg cattggtctg
     2341 ggtgtgggct ggtctacgct ggtagcggcg gagctgattg ccgcgacacg cggtttagga
     2401 tttatggttc agtcagcggg tgaatttctc gcgactgacg tggtactggc ggggatcgcg
     2461 gtgattgcga ttatcgcctt tcttttagaa ctgggtctgc gcgcgttaca gcgccgtctg
     2521 acgccctggc atggagaagt acaatgagtg aacgtctgag cattaccccg ctggggccgt
     2581 atatcggcgc acaaatttcg ggtgccgacc tgacgcgccc gttaagcgat aatcagtttg
     2641 aacagcttta ccatgcggtg ctgcgccatc aggtggtatt tctgcgcgat caagctatta
     2701 cgccgcagca gcaacgcgcg ctggcccagc gttttggcga actgcatatt caccctgttt
     2761 accctcatgc cgaaggggtt gacgagatca tcgtgctgga tacccataac gataatccgc
     2821 cggataacga caactggcat accgatgtga catttattga aacgccaccc gcaggggcga
     2881 ttctggcagc taaagagtta ccttcgaccg gcggtgatac gctctggacc agcggtattg
     2941 cggcctatga ggcgctctct gttcccttcc gccagctgct gagctggctg cgtgcggagc
     3001 atgatttccg taaatcattc ccggaataca aataccgcaa aaccgaggag gaacatcaac
     3061 gctggcgcga ggcggtcgcg aaaaatcctc cgttgctaca tccggtggtg cgaacgcatc
     3121 cggtgagcgg taaacaggcg ctgtttgtga atgaaggctt tactacgcga attgtcgatg
     3181 tcagcgagaa agagagcgaa gccttgttag gctttttatt cgcccatatc actaaaccgg
     3241 agtttcaggt gcgttggcgc tggcaaccga atgatattgc gatttgggat aaccgcgtga
     3301 ctcagcacta tgccaatgcc gattacctgc cacagcggcg gattatgcat cgggcgacga
     3361 tccttgggga taaaccgttt tatcgggcgg ggtgatacga gtgcggacgg ttccctcgcc
     3421 cccttgggga gagggttagg gtgagggggc gttcactgta ctttcaacag gttaactccc
     3481 cctttctgag aggaaaaaaa ttaacgcaga atcttcttct cagccaaatc cagcgcaaag
     3541 tagctgaaaa tcagatccgc acccgcacgc ttaattgaac ctaagctttc gagcacgact
     3601 ttctcttcat ctatagcacc cgccagcgcg gcgaacttaa tcatcgcgta ctcaccgctc
     3661 acctgatacg cgccaatcgg caattcagta cgttcacgca gctcacgcac gatgtcgagg
     3721 tacgctccag caggtttaac catcaggcag tctgcgccct gggcttcatc cagcagtgac
     3781 tcacgaatcg cctcgcgacg gttcattggg ttcatctgat agcttttgcg gtcgcctttt
     3841 aatgcgcttc ctgcagcttc acggaacggg ccataaaagg aagaggcgaa cttggtcgaa
     3901 tacgacataa tcgccgtatc tttaaagcct gcagcgtcca gcgcctgacg aatcgcctgt
     3961 acctggccgt ccatcgcggc ggaaggggcg atgaagtctg cgcctgcagc agctgcaacc
     4021 acggcttgct tgcctaaatt ttccagagtc gcgtcgttgt cgacgccatg ctcgcacagc
     4081 acaccgcagt gaccgtgaga agtgtattca cagaaacaag tgtctgacat gacgatcatt
     4141 tctggcacag tctgcttgca gatgcgcgac atacgcgcca ccagtccatc ttcccgccag
     4201 gcgtcgctgc cggtttcatc ggtatggtga gagatgccaa aagtcattac ggaacggata
     4261 ccggcgttgg cgatgcgttc aatttcgcgt gccagatgtt tctctggaat gcgcatcacg
     4321 cctggcatgg cttcaacggc tttgtagtcg tcaatttctt cttcaacaaa gatcggcaac
     4381 accaggtcgt taaggctaag tgttgtctct tcaaacatag cgcgcagcgc aggagatttg
     4441 cgcaggcgac gagggcgttg gattaagtct gtcatggtct gcctgatgtt tgtggaatta
     4501 aggtgcacag tatacctgaa gcggggcagg agtgttttac gaaagttgtc gcgatgttga
     4561 caagaagaga atgaaagaga gaccaggaca tctttgtaat agaaatagca ataatttcat
     4621 attcactgaa aatattttta atctttattt atagctcttg gttattattt tttggcgttt
     4681 ggttgtgctg ctataaattg aataattcaa actttgttgt atattgataa gatatttatc
     4741 tggtttgtat ttttgatatt tataacgcgt atgcatcaaa aataacatta tctgcaacat
     4801 actgaaatat cgtcctttta tcaatttgat gcgtgttttt ctccataaat tatatatgtc
     4861 cacattcgga cttaggggaa agaataattg aaccattcgt ctgtaacgca gcataat
//
LOCUS       DAJTYM010000178         4865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTYM010000178 DAJTYM010000000
VERSION     DAJTYM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            145..2706
                     /gene="mutS"
                     /locus_tag="OO575_003836"
     CDS             145..2706
                     /gene="mutS"
                     /locus_tag="OO575_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6666611.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2812..3464
                     /gene="pphB"
                     /locus_tag="OO575_003837"
                     /pseudo
     CDS             2812..3464
                     /gene="pphB"
                     /locus_tag="OO575_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3515..4282)
                     /locus_tag="OO575_003838"
     CDS             complement(3515..4282)
                     /locus_tag="OO575_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6666612.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4478..4865
                     /locus_tag="OO575_003839"
                     /pseudo
     CDS             4478..4865
                     /locus_tag="OO575_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT         1196 a         1265 c         1256 g         1148 t
ORIGIN      
        1 ctttcccgga accagcatca agaactcgcc gttcgcttct tcccctgaaa tgattaactc
       61 cggtatcatg tgcgccttat gtgattacaa cgaaaataaa aaccatcaca ccccatttaa
      121 tatcagggaa ccggacataa ccccatgagt gcaatagaaa atttcgacgc ccatacgccc
      181 atgatgcagc agtatctcaa gctgaaagcc cagcatcccg agatcctgct gttttaccgg
      241 atgggtgatt tttatgaact gttttatgac gacgcaaaac gcgcgtcgca actgctggat
      301 atttcactga ccaaacgcgg tgcttcggcg ggagagccga tcccgatggc ggggattccc
      361 taccatgcgg tggaaaacta tctcgccaaa ctggtgaatc agggagagtc cgttgccatc
      421 tgcgaacaaa ttggcgatcc ggcgaccagc aaaggtccgg ttgagcgcaa agttgtgcgt
      481 atcgttacgc caggcaccat cagcgatgaa gccctgttac aggagcgtca ggacaacctg
      541 ctggcggcta tctggcagga cagcaaaggt ttcggctacg cgacgctgga tatcagctcc
      601 gggcgttttc gcctgagcga accggccgac cgcgaaacga tggcggcaga gctgcaacgc
      661 tctaatccgg cggaactgct gtatgcagaa gatttcgccg agatgtcgct gattgaaggc
      721 cgtcgcggcc tgcgccgtcg cccgctgtgg gagtttgaaa tcgacaccgc gcgccagcag
      781 ttgaatctgc aatttggcac ccgcgatctg gtcggttttg gcgtcgagaa cgcgccgcgc
      841 ggactttgtg ctgccggttg tctgttgcag tatgcgaaag atacccaacg cacgaccctg
      901 ccgcatattc gttctatcac catggaacgt gagcaggaca gcatcattat ggatgccgcg
      961 acacgtcgta acctggaaat tactcagaac ctggcgggcg gtgcggaaaa tacgctggct
     1021 tctgtgctcg actgcaccgt cacgccgatg ggtagtcgta tgctgaaacg ctggctgcat
     1081 atgccagtgc gcgatacccg cgtgttgctt gagcgccagc aaactattgg cgcattgcag
     1141 gatttcaccg ccgagttgca gccggtactg cgtcaggtcg gcgacctgga acgtattctg
     1201 gcacgtctgg ctttacgaac tgctcgccca cgcgatctgg cccgtatgcg ccacgctttc
     1261 cagcaactgc cggagctgcg tgcgcagtta gaaactgtcg atagtgcacc ggtacaggcg
     1321 ctacgtgaga agatgggcga gtttgccgag ctgcgcgatc tgctggagcg agcaatcatc
     1381 gacacaccgc cggtgctggt acgcgacggt ggtgttatcg catcgggcta taacgaagag
     1441 ctggatgagt ggcgcgcgct ggctgacggc gcgaccgatt atctggagcg tctggaagtc
     1501 cgcgagcgtg aacgtaccgg cctggacacg ctaaaagttg gctttaatgc ggtgcacggc
     1561 tactacattc aaatcagccg tgggcaaagc catctggcac ccatcaacta catgcgtcgc
     1621 cagacgctga aaaacgccga gcgctacatc attccagagc taaaagagta cgaagataaa
     1681 gttctcacct caaaaggcaa agcactggca ctggaaaaac agctttatga agagctgttc
     1741 gacctgctgt tgccgcatct ggaagcgttg caacagagcg cgagcgcgct ggcggaactc
     1801 gacgtgctgg ttaacctggc ggaacgggcc tataccctga actacacctg cccgaccttc
     1861 attgataaac cgggcattcg cattaccgaa ggtcgccatc cggtagttga acaagtactg
     1921 aatgagccat ttatcgctaa cccgctgaat ctgtcgccgc agcgccgtat gttgattatt
     1981 accggcccga acatgggcgg taaaagtacc tatatgcgcc agaccgcact gattgcgctg
     2041 atggcctaca tcggcagcta tgtaccggca caaaaagtcg agattggacc tatcgatcgc
     2101 atctttaccc gcgtaggcgc ggcagatgac ctggcttccg ggcgctcaac ctttatggtg
     2161 gagatgactg aaaccgccaa tattttacat aacgccaccg aatacagtct ggtgttaatg
     2221 gatgagatcg ggcgtggaac gtccacctac gatggtctgt cgctggcgtg ggcgtgcgca
     2281 gaaaatctgg cgaataagat taaggcattg acgctgtttg ccacccacta tttcgagctg
     2341 acccagctac cggagaaaat ggaaggcgtc gccaacgtac atctcgatgc gctggagcac
     2401 ggcgacacta tcgcctttat gcacagcgtg caggacggcg cggcgagcaa aagctacggt
     2461 ctggcggttg ctgctctggc gggcgtaccg aaagaggtta ttaagcgcgc acggcaaaaa
     2521 ctgcgtgagc tggaaagcat ttcgccgaac gccgccgcga cgcaagtgga tggtacgcag
     2581 atgtctttgc tgtcagtgcc agaagaaact tcgcctgcgg tcgaagcact ggaaaatctc
     2641 gacccggatt cactgactcc gcgtcaggcg ctggagtgga tttatcgctt gaagagtctg
     2701 gtgtaataac aattcccgat agtcttttgc tatcgggaat attaacgaca actgacgaat
     2761 caaataaaaa taccctgtat aataggaaag cttattttac agggtaaaac catgccatct
     2821 acacgctacc aaaaaatcaa tgcccatcac tatcgccata tatgggtcgt tggtgatatt
     2881 catggtgaat atcagttatt acaatcccgc ttacatcaac tctctttttt ccccgaaacc
     2941 gacttactta tttctgtcgg cgataatatt gatcgtgggc cggagagtct tgacgtcctg
     3001 cgcctgctaa accagccctg gtttatctcc gttaaaggca accacgaagc aatggcgctg
     3061 gatgcgttcg cgaccggcga tggcaatatg tggcttgcca gcggtggtga ctggtttttc
     3121 gatttaaatg attgcaacaa gaggcaatag ttctgttgct gaaattccat caccttccac
     3181 atattattga aatcactaac gacataataa aatatgtcat cgcacatgca gattatccgg
     3241 gtgatgaata tctctttggc aaagaaatag cggagagcga attactctgg cctgtttatc
     3301 gtgtgcagaa atcgcttaat ggcgagttac aacaaataaa cggcgctgat tattttatat
     3361 ttggacatat gatgtttgat aacattcaga cgttcgctaa ccagatttat attgataccg
     3421 gatcgccgaa aagtgggcgg ctgtcatttt ataaaataag gtagtctcat gcttcttctg
     3481 tgaagcatga gtaaccagat gttatttcag gccattattc atttttcgct accagcaaag
     3541 agagatcctg cttcgccagc gcgcgactgg cactctccgg caaaccgtcg tctgtaataa
     3601 tctgatcaaa ctcgcttaat ggtaacgcca gccatgtcgc cacctgacca tatttcgtcg
     3661 catcacagac caaaactcgc tggcggctgg cactggcaat cgcccgtttc accgtgactt
     3721 tatcttccgc tggcgtagaa atcccccgca cactccatga cgatgcagaa ataaaagcct
     3781 gatcaatcat caggctgcac agcatggtcg cagcggcttc cccgacacag gaacggtttt
     3841 cccgacacac tgcaccgcca gtgtgaataa ttgtgcaatt actgttgtcg agcaagtagt
     3901 tcgcaataac gaaatcgttt gtgaccacag tcagtgactc catgtgaatc agatgctgtg
     3961 ctatcgctaa cgtggtcgtt cccgcatcca gatagataca acttcccggc agaacaagac
     4021 ttgccgccag cttgccaata gccgcttttt gcgtcattgc cagcgcagtt tttacctgat
     4081 gagaaggttc atgcgccacg cgtcccggag actggacgcc tccggacacc agcacaacgg
     4141 ctccctgctg ctccagtttt tgtaaatccc gacgaatggt catatgtgac acattaattc
     4201 tgtccgttag ttcagcaata ctgacaatgc ctttttcagc taccatctca aggatgattt
     4261 ggcgacgctc tacgggtatc aacttttgct ccttcctttg tcctgctgac attctacgct
     4321 atttgcctgc gaaacgtgcg cggcgcaact aacgcttagt tcacataaaa taacacaaaa
     4381 tgttaattta tgtgaatcat ctcaccatat cgttatctcc cagcgcttat gttcacaata
     4441 tcaaacaaaa tatcacttaa attaacaagg agagcagatg aaaacgggat ctgagtttca
     4501 tgtcggtatc gttggcttag ggtcaatggg aatgggagca gcactgtcat gtgtccgcgc
     4561 aggtttttct acctggggcg cagacctgaa cagcaatgcc tgcgctacgt tgaaagaggc
     4621 aggtgcttgc ggggtttctg ataacgccgc gacgtttgcc gaaaaactgg acgctctgct
     4681 ggtgctggtg gtcaatgcgg cctaggttaa acaggtgctg tttggtgaaa caggcgttgc
     4741 acaacatctg aaacccggta cggcagtaat ggtttcttcc actatcgcta gtgctgatgc
     4801 gcaagaaatt gctaccgctc tggctggatt cgatctggaa atgctggatg cgccagtttc
     4861 tggtg
//
LOCUS       DAJTYM010000179         4856 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTYM010000179 DAJTYM010000000
VERSION     DAJTYM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..525)
                     /gene="bamD"
                     /locus_tag="OO575_003840"
     CDS             complement(<1..525)
                     /gene="bamD"
                     /locus_tag="OO575_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6666613.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKGRLA"
     gene            660..1640
                     /gene="rluD"
                     /locus_tag="OO575_003841"
     CDS             660..1640
                     /gene="rluD"
                     /locus_tag="OO575_003841"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6666614.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1637..2368
                     /gene="pgeF"
                     /locus_tag="OO575_003842"
     CDS             1637..2368
                     /gene="pgeF"
                     /locus_tag="OO575_003842"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6666615.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2498..>4856
                     /gene="clpB"
                     /locus_tag="OO575_003843"
     CDS             2498..>4856
                     /gene="clpB"
                     /locus_tag="OO575_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6666616.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRL"
BASE COUNT         1203 a         1176 c         1331 g         1146 t
ORIGIN      
        1 cgccagacga cctttcagga ataccagacg tttggtggca tcggtagtgt actgactgtt
       61 cggatagccg cgcaccagtt tggaaaagtc actaaacgca gctcgtgcat gttgaggatc
      121 gcgatcgcta cggtcaacgc caaagaaccc ttgcagcgca ctgtcatcca gcgccatatt
      181 ggtcaggcca cgcatgtaca tgacataatc gatattcgga tgggtcgggt taaggcgaat
      241 aaaacgatcg atggcagcct gcgctaacgg caaatcggcg tttttatagt aggcgtagat
      301 gagatccagc tgcacctgct gcgaatacgg accaaacgga tagcgattat ctaacgcttc
      361 cagttgcgtt attgcctgtc tccagttacc gtcctgcagc ttttgttgtg cagtcgcgta
      421 aatttcattt ggcggattat caggtacttc ttcctttgac cccgagcaac ccgccaaaaa
      481 caggcttagt gtggcggctg ccaccagata tttcatgcgc gtcatgacgt tttgactttc
      541 ctcaaaatgt aatacgggag attctctgtt cctgcacccg gttaagacca gctacaatag
      601 cacactatat taaacggcaa agccgtaaaa ccccaacgat aaacgaagaa gcagtatata
      661 tggcacaacg agtacagctc actgcaacgg tgtccgaaaa ccaactcggt caacgcttag
      721 atcaggcttt ggccgaaatg ttcccggatt attcacgttc gcgaataaaa gaatggatcc
      781 tcgaccagcg cgtgctggta aacggcaaag tttgtgataa gccgaaagaa aaagtattgg
      841 gtggcgagca ggttgccatc aacgctgaga ttgaagaaga agcgcgtttt gaaccgcagg
      901 atatccctct ggatatcgtc tatgaagatg aagatatcat cgtcattaat aaaccgcgcg
      961 acctagtggt acatcctggc gcgggtaacc cggatggcac ggtactgaat gcgttgcttc
     1021 attactatcc gcccattgcc gatgtaccac gtgcgggcat cgtccatcgt ctggataaag
     1081 acaccactgg cctgatggtt gtggcaaaaa ccgttccggc tcagacgcgt ttagtcgaat
     1141 ctttgcaacg gcgtgaaatt actcgtgagt atgaagcggt ggcgattggt catatgaccg
     1201 ctggcggtac agtagatgaa cccatcagcc gccacccgac caaacgtact cacatggcgg
     1261 tgcatccgat gggcaaaccg gcggtgactc actatcgcat catggaacac ttccgtgtgc
     1321 acacgcgtct gcggttgcgt ctggaaactg gacgtacgca ccagatccgc gtgcatatgg
     1381 cccatatcac tcatccgctg gtgggcgatc cggtttatgg tggccgtccg cgtccgccaa
     1441 aaggtgcttc ggaagcattt atctccacgc tgcgtaagtt tgaccgccag gcgcttcatg
     1501 caaccatgct gcgtctttat cacccgatct ccggcatcga aatggaatgg catgcgccta
     1561 ttccacaaga tatggtggag ctgattgagg tgatgcgcgc cgatttcgaa gagcataagg
     1621 atgaagtgga ctggttatga gtaagctgat tgtcccgcag tggccgctgc caaaaggtgt
     1681 tgcggcctgt agctccactc gtatcggcgg cgtgagcttg cccccgtatg actcactcaa
     1741 cctcggtgcc cattgtggcg ataacccgga tcacgttgag gagaatcgca agcgactttt
     1801 tgctgcgggc aatttgcctt ctaaaccggt ctggcttgag caggtacacg gcaaagatgt
     1861 gcttaagctt actggcgaac cttatgcctc aaaacgagca gatgcctctt atagcaatac
     1921 gcctggcacg gtttgcgcag tgatgactgc cgactgcctc cctgtgctgt tttgcaatcg
     1981 agcgggaacg gaagtcgccg ccgttcatgc tggctggcgt ggactgtgcg caggcgtgct
     2041 ggaagagacg gtttcctgtt ttgctgataa acctgaaaac attctcgcct ggttagggcc
     2101 ggcaattggt ccacgcgcgt tcgaagtggg ggcggaggtt cgcgaggcgt ttatggcagt
     2161 agacgctaaa gcaagtgcag ctttcattca gcatggtgat aagtatctgg cggatattta
     2221 tcagcttgcc cggcagcgtc tggcgaacgt gggtgttgag caaattttcg gcggcgaccg
     2281 ttgtacatat acggaaaatg agactttctt ctcttatcgt cgcgacaaga ccaccggtcg
     2341 tatggcaagt ttcatttggc tgatataacc taaagaatca agacgatccg gtacgcgtga
     2401 ttttcttttc acattaatct ggtcaataac cttgaataat tgagggatga cctcatttaa
     2461 tctccagtag caactttgat ccgttatggg aggagttatg cgtctggatc gtcttactaa
     2521 taaattccag cttgctcttg ccgatgccca atcacttgca ctcgggcacg acaaccaatt
     2581 catcgaacca cttcatttaa tgagcgccct gctgaatcag gaagggggtt cggttagtcc
     2641 tttattaaca tccgctggca taaatgctgg ccagttgcgc acagatatca atcaggcatt
     2701 aaatcgttta ccgcaggttg aaggtactgg tggtgatgtc cagccatcac aggatctggt
     2761 gcgcgttctt aatctttgcg acaagctggc gcaaaaacgt ggtgataact ttatctcgtc
     2821 agaactgttc gttctggcgg cacttgagtc tcgcggcacg ctggccgaca tcctgaaagc
     2881 aacaggggcg accaccgcca acattactca agcgattgaa caaatgcgtg gaggtgaaag
     2941 cgtgaacgat caaggtgctg aagaccaacg tcaggctttg aaaaaatata ccatcgacct
     3001 taccgaacga gccgaacagg gcaaactcga tccggtgatt ggtcgtgatg aagaaattcg
     3061 ccgtaccatt caggtgctgc aacgtcgtac taaaaataac ccggtactga ttggtgaacc
     3121 cggcgtcggt aaaactgcca tcgttgaagg tctggcgcag cgtattatca acggcgaagt
     3181 gccggaagga ttgaaaggcc gccgcgtact ggcgctggat atgggcgcgc tggtggctgg
     3241 ggcgaaatat cgcggtgagt ttgaagaacg tttaaaaggc gtgcttaacg atcttgccaa
     3301 acaggaaggc aacgtcatcc tgtttatcga cgaattacat accatggtcg gcgcgggtaa
     3361 agccgatggc gcaatggacg ccggaaacat gctgaaaccg gcgctggcgc gtggtgaatt
     3421 gcactgcgta ggtgccacga cgcttgacga atatcgccag tacatcgaaa aagatgctgc
     3481 gctggaacgt cgtttccaga aagtgtttgt tgccgagcct tctgttgaag ataccattgc
     3541 gattctgcgt ggcctgaaag aacgttacga attgcaccac catgtgcaaa ttactgaccc
     3601 ggcaattgtt gcagcagcga cgttgtctca tcgctacatt gctgaccgtc agctgccgga
     3661 taaagccatc gacctgatcg atgaagcagc atccagcatt cgtatgcaga ttgactcaaa
     3721 accagaagaa ctcgaccgac tcgatcgtcg tatcatccag ctcaaactgg aacaacaggc
     3781 gttaatgaaa gagtctgatg aagccagtaa aaaacgcctg gatatgctca acgaagaact
     3841 gagcgacaaa gaacgtcagt actccgagtt agaagaagag tggaaagcag agaaggcatc
     3901 gctttctggt acgcagacca ttaaagcgga actggaacag gcgaaaatcg ctattgaaca
     3961 ggctcgccgt gtgggggacc tggcgcggat gtctgaactg caatacggca aaatcccgga
     4021 actggaaaag caactggaag ccgcaacgca gctcgaaggc aaaactatgc gtctgttgcg
     4081 taataaagtg accgatgccg aaattgctga agtgctggcg cgttggacgg ggattccggt
     4141 ttctcgcatg atggaaagcg agcgcgaaaa actgctgcgt atggagcaag aactgcacca
     4201 tcgcgtcatt ggtcagaacg aagcggttga tgcggtatct aacgctattc gccgtagccg
     4261 tgcggggttg gcggatccaa atcgcccgat tggttcattc ctgttcctcg gcccaacggg
     4321 tgtggggaaa actgagcttt gtaaggcgct ggcgaacttt atgtttgata gcgacgaggc
     4381 gatggtccgt atcgatatgt ccgaatttat ggagaaacac tcggtgtctc gtctggttgg
     4441 tgcgcctccg ggatatgtcg gttatgaaga aggtggctac ctgaccgaag ctgtgcgtcg
     4501 tcgtccgtat tccgtcatcc tgctggatga agtggaaaaa gcgcatccgg atgtcttcaa
     4561 tattctgttg caggtactgg atgatgggcg tctgactgac gggcagggga gaacggtcga
     4621 cttccgtaat acggttgtca ttatgacctc taacctcggt tccgatctga ttcaggaacg
     4681 cttcggtgaa ctggattatg cgcacatgaa agagctggtg ctcggtgtgg ttagccataa
     4741 cttccgtccg gaattcatta accgtattga tgaagtggtg gtcttccatc cgctgggtga
     4801 acagcacatt gcctcgattg cgcagattca gttgaaacgt ctgtacaaac gtctgg
//
LOCUS       DAJTYM010000180         4674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTYM010000180 DAJTYM010000000
VERSION     DAJTYM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..212
                     /locus_tag="OO575_003844"
                     /pseudo
     CDS             1..212
                     /locus_tag="OO575_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            355..576
                     /locus_tag="OO575_003845"
                     /pseudo
     CDS             355..576
                     /locus_tag="OO575_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(597..659)
                     /locus_tag="OO575_003846"
                     /pseudo
     CDS             complement(597..659)
                     /locus_tag="OO575_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1109..2521
                     /gene="ospC1"
                     /locus_tag="OO575_003847"
     CDS             1109..2521
                     /gene="ospC1"
                     /locus_tag="OO575_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6666617.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2625..2774)
                     /locus_tag="OO575_003848"
                     /pseudo
     CDS             complement(2625..2774)
                     /locus_tag="OO575_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2850..4547
                     /gene="ospD3"
                     /locus_tag="OO575_003849"
     CDS             2850..4547
                     /gene="ospD3"
                     /locus_tag="OO575_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6666618.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1629 a          840 c          868 g         1337 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatgggggcg
      121 gtgaagaatt atctaacctt gtcttgttac atccaaattg ccatcgacag ttacacagtt
      181 gtgaagccgg ctctctcaaa aagggcttat aaaggcttga gcggtgtacc gggaaaccgg
      241 tatgcaccgt tcttagggga gggcagtcca gcaatgaact gtccttaccc gacaaacatc
      301 caaaacgaac ggaacgtgct ggaatccgca tataacccgt tgtaaagtga ggaaatggac
      361 acctcacttg ctcatgagaa cgcccgcctg cgggcactgt tgcagacgca acaggacact
      421 atccaccaga tggctgaata caaccgcctg ctctcacagc ggatggcggc ttatgcttcc
      481 gaaatcaacc gactgaaggc gctggttgcg aaactgcagc gtatgcagtt cggtaaaagc
      541 tcagaaaaac ttcgcgcaaa aaccgaacgg cagattccat tttcacgggc gatctacgcc
      601 acacaggctc tgggttgcag tgactgctcc attaaggcga ttttgaactc ataagggaag
      661 taacaaaaaa actgcaaaag tacaataatt atatatggcc aaactaacct ctattaaaaa
      721 acatcagttt ttcacactta gttgagcttc atgaaaaaat catcgaggat ttcattgcag
      781 aatacacatt ccactaactg gaagcactcc ataagaggaa tcagagaaag tatcgttttt
      841 ttatagtaaa attcattgct gttcaataaa attgatatag ttattaaatt atgaaaataa
      901 tttcacaaaa aagattaact tgagttctta agcatcggat tttagatatt ttgaaatatc
      961 tggcatcaca taacatcaaa agtatataat actcttatct aattacatgc agtatattgt
     1021 atatatactc aaaaattgta attcataccc tgctatatct tgtagggaaa gatattaatt
     1081 aaaactgttt tcatataagg ttcattttat gaatatatca gaaacactga actcagcaaa
     1141 tacccaatgc aatatagatt ctatggataa cagattacat acattgtttc caaaagtgac
     1201 atcagtgcga aacgctgcac aacaaactat gccagatgaa aaaaatttaa aagatagtgc
     1261 aaatattatt aaaagtttct ttaggaaaac tatagcagca cagagttata gtagaatgtt
     1321 ctctcaaggc tctaacttta aatctttaaa tatagcaatt gatgcaccat cagacgctaa
     1381 agcctcattt aaggctattg agcaccttga cagattatcg aagcattata tatctgaaat
     1441 aagggaaaaa cttcatcctc tttctgcaga ggaactcaat ttgctttcgc taattattaa
     1501 ttctgattta atcttcagac atcaaagtaa ttctgatttg tctgataaaa ttttaaacat
     1561 taagtcattc aataaaattc agtctgaagg aatatgcaca aaacgaaaca catacgctga
     1621 tgatataaaa aaaatagcta atcatgactt tgtgtttttt ggcgttgaaa tctctaacca
     1681 tcagaaaaaa caccccctga atacaaaaca tcacactgtt gattttggtg caaatgcgta
     1741 tatcattgat catgactctc catatggata tatgacatta accgatcact ttgataatgc
     1801 tattccacct gttttttacc atgagcacca atcattttta gataaatttt cagaggttaa
     1861 taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac caatattcaa
     1921 tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta gaaaaagtga
     1981 agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg atctggatag
     2041 aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa gtacagaaaa
     2101 cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag catctaatta
     2161 cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc tttttctttc
     2221 gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg agataactag
     2281 acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac ttagcgctca
     2341 taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg atgtaaacac
     2401 aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa aatatgagaa
     2461 tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata aatatattta
     2521 atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg atatattgtt
     2581 tattgtcagt atggctcaat gtgataatag ttggaaagtt tgatgggttt cgccccgttg
     2641 tagcggtagt cgaactggaa ggtcttcagg cactgcttac agcgatagag cagcccccct
     2701 gaactggaat ggccgttccg gatacccccc tgagtttcag agtaacggtg acaaaccaca
     2761 tcaatctttg ccatcaatca tccaaagggc aaagagtaca acaacactaa gtctgcgtca
     2821 caacccatca atgaaaggaa tatatacata tgccatcagt aaatttaatc ccatcaagga
     2881 aaatatgttt gcaaaatatg ataaataaag acaacgtctc tgttgagaca atccagtctc
     2941 tattgcactc aaaacaattg ccatattttt ctgacaagag gagtttttta ttaaatctaa
     3001 attgccaagt taccgatcac tctggaagac ttattgtctg tcgacattta gcttcctact
     3061 ggatagcaca gtttaacaaa agtagtggtc acgtggatta tcatcacttt gcttttccgg
     3121 atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa ggctattaat gtgcctgcta
     3181 ttatttattt tgttgaaaac ggttcatggg gagatattat tttttatatt ttcaatgaaa
     3241 tgatttttca ttccgaaaaa agcagagcac tagaaataag tacatcaaat cacaatatgg
     3301 cattaggctt gaagattaaa gaaactaaaa atggggggga ttttgtcatt cagctttatg
     3361 atcccaacca tacagcaact catttacgag cagagtttaa caaatttaac ttagctaaaa
     3421 taaaaaaact gactgtagat aattttcttg atgaaaaaca tcagaaatgt tatggtctta
     3481 tatccgacgg tatgtctata tttgtggaca gacatactcc aacaagcatg tcctccataa
     3541 tcagatggcc tgataattta cttcacccca aagttattta tcacgcgatg cgtatgggat
     3601 tgactgagct aatccaaaaa gtaacaagag tcgtacaact atctgacctt tcagacaata
     3661 cgttagaatt acttttggca gccaaaaatg acgatggttt gtcaggattg cttttagctt
     3721 tacaaaatgg gcattcagat acaatcttag catacggaga actcttggaa acttctggac
     3781 ttaaccttga taaaacggta gaactactaa ctgcggaagg aatgggagga cgaatatcgg
     3841 gtttatccca agcacttcaa aatgggcatg cagaaactat caaaacatac ggaaggcttc
     3901 tcaagaagag agcaataaat atcgaataca ataagctgaa aaatttgctg accgcttatt
     3961 attatgatga agtacacaga cagatacccg gactaatgtt tgctcttcaa aatggacatg
     4021 cagatgctat acgcgcatac ggtgagctca ttcttagccc ccctctcctc aactcagagg
     4081 atattgtaaa tttgctggcc tcaaggagat atgacaatgt tcccggactt ctgttagcat
     4141 tgaataatgg acaggctgat gcaatcttag cttatggtga tatcttgaat gaggcaaaac
     4201 ttaacttgga taaaaaagca gagctgttag aagcgaaaga ttctaatggt ttatctggat
     4261 tgtttgtagc cttgcataat ggatgtgtag aaacaattat tgcttatggg aaaatacttc
     4321 acactgcaga ccttactcca catcaggcat caaaattact ggcagcagaa ggcccaaatg
     4381 gggtatctgg attaattata gcttttcaaa ataggaattt tgaggcaata aaaacttata
     4441 tggaaataat aaaaaatgaa aatattacac ctgaagaaat agcagaacac ttggacaaaa
     4501 aaaatggaag tgattttcta gaaattatga agaatataaa aagctgaata ttatctcgtg
     4561 gtgatacact gaatttgagg taaccggctc atttaaaccg tctggtctgt tacctccggt
     4621 tccacaaaaa taatgtccat cattttgaag tggacactat cgtatggaat accg
//
LOCUS       DAJTYM010000181         4611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTYM010000181 DAJTYM010000000
VERSION     DAJTYM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            213..1121
                     /locus_tag="OO575_003850"
     CDS             213..1121
                     /locus_tag="OO575_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6666619.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1167..3158)
                     /gene="tkt"
                     /locus_tag="OO575_003851"
     CDS             complement(1167..3158)
                     /gene="tkt"
                     /locus_tag="OO575_003851"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6666620.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3436..4194
                     /gene="loiP"
                     /locus_tag="OO575_003852"
                     /gene_synonym="yggG"
     CDS             3436..4194
                     /gene="loiP"
                     /locus_tag="OO575_003852"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6666621.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4400..>4611)
                     /locus_tag="OO575_003853"
     CDS             complement(4400..>4611)
                     /locus_tag="OO575_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HCR6666622.1"
                     /translation="FAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAYDQ
                     SEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1097 a         1213 c         1214 g         1087 t
ORIGIN      
        1 gtatttgtta tgttcatcac aaaatcaaca ttttgtttgt tattgtattt gtgatgcata
       61 tctctattta tttattgaat gttcgaaaac atacttttct aatgcaatgt tttttcgcta
      121 taccccttta tagattctga attctcacct gtcggctgtt ttctcctcat ctcatgtgac
      181 atattcccat actttactca aggaagattg tcatgaaagc acctgtttca ttaacctcat
      241 ttcgcccaca aaaatctctt gctatcgcca ttggcgtgct tgccgtggtt gtgctgccat
      301 ttctctcgta ttacaccgtt aatgaaggtg aacgcggcat tttgctgcgc tacggaaaaa
      361 tcgtaaaagt tgccgaaccg gggctgggtt ttaagatccc ttttatggaa agcgtggaga
      421 aaatttccac gcgtaatcag gctgtggtct atcagggatt acaggcctac agccgcgacc
      481 agcaaccggc acaaatgaca gtatcagtca gcttccacat taaaccttca gaagcaggcg
      541 cggtttatac cacctacaac actattgaag cattaaaaga tcgtctaatt gtccgtcagt
      601 tgccaactca gctggagaac attttcggcc aatataccgc catcagcgcc gtacaggatc
      661 gtacaaaatt ggttcaggat ctgcaaaatg ccatgcgcaa agccgttgtt gggccagtgg
      721 taattgacgg ggtccagatt gaaaatattg atttttctga cgcttatgaa aagtcgattg
      781 aaaaccgaat gaaagcagaa gtcgccattg ccacgcgtaa gcaaaatctg gaaacagaga
      841 aaatccaggc gcaaatagcg gtaactcagg cccaggcaga agcagacagt aaactggcgg
      901 cagccaaagc agaagcggaa actattcgcg taagaggcgc ggcagaggct gaaactattc
      961 gtttaaaaag tgcggctgaa gcagaagcaa tccgcctgcg cggtgaagct ctgcgggata
     1021 atcccggact tgtagccctc accactgccg aacgttggga cggtaaactg cccgatacca
     1081 tgatccccgg cagcacagtt ccgtttatct ctactaagta aaaaaagggc cgctttcgcg
     1141 gtccttctca caaaacgtct tactgattac agcagcgctt ttgctttcgc aacaacgtta
     1201 tcaacagtga agccgaactc ttcaaacagc agctctgccg gagcagattc accgaaggtg
     1261 gtcatgccga cgatagcgcc gttcaggcca acatacttgt accagtagtc agcaataccc
     1321 gcttctaccg caacgcgtgc agtaaccgct ttcggcagta cggattcacg gtaagccgca
     1381 tcctgcttgt caaatgcgtc ggtagacggc atggacacca cgcgcgcttt cacgccttcg
     1441 gcagtcagtt tttcgtaggc agcaaccgcc agttcaactt cagaaccggt ggcgatgaag
     1501 atcagttccg gctgaccagc acagtctttc agcacataac caccgcgcgc gatgtttgcc
     1561 agctgctctt cagtacgttc ctgctgcgcc aggttctgac gggagaggat aagcgcagtc
     1621 gggccgtcct gacgctcaac gccgtatttc cacgcgaccg cggattcaac ctggtcacac
     1681 ggacgccatg tagacatgtt cggggtcacg cgcagagaag cgacctgctc taccggctgg
     1741 tgagtcgggc catcttcgcc cagaccgatg gagtcgtggg tgtaaaccat cacctggcgc
     1801 tgtttcatca gcgcagccat acgtacggcg ttacgtgcgt attccacgaa catcaggaag
     1861 gtggaggtgt acggcaggaa gccaccgtgc agggagatac cgttagcaat cgcggtcata
     1921 ccgaactcgc gaacaccgta gtggatgtag ttacctgcag catcttcgtt gattgcttta
     1981 gaaccagacc acagggtcag gttagacggt gccaggtcag cagagccgcc gaggaattca
     2041 ggcaacagcg ggccgaacgc ttcgatagca ttctgcgacg ctttacggct ggcgattttc
     2101 gccggattag cctgcagttt agcgataaac tctttcgctt tggcgtcgaa gtcagacggc
     2161 atttcgcctt tcatacggcg ggtaaattca gccgcttcct gcggataagc tttcgcgtaa
     2221 gccgcaaact tctcattcca tgcagattct ttcgcctggc ctgcttcttt cgcatcccac
     2281 tgagcataga tttcagacgg gatttcgaac ggtgcatatt tccagcccag ctgttcgcgg
     2341 gtcagggcaa tttcagcgtc gcccagcggc gcaccgtggg agtcgtgagt accggctttg
     2401 ttcggggaac cgaaaccgat gatggttttg cacatcagca gggacggttt gtcagtcact
     2461 gcgcgcgctt cttcaactgc gcgtttgatg gatgccgcgt catgaccgtc gatgtcgcga
     2521 ataacgtgcc agccgtaagc ttcgaaacgc attgctgtgt cgtcggtgaa ccagccttca
     2581 acgtgaccat cgatagaaat accgttgtca tcgtagaacg caatcagttt acccagcttc
     2641 agcgtacccg ccagagagca aacttcgtgg gagatgcctt ccatcatgca gccgtcgccc
     2701 atgaaggcgt aggtgtagtg gtcgacaatg tcgtggcccg gacggttaaa ctgcgccgcc
     2761 agcgtttttt ctgcaatcgc cataccgact gcgttggcaa taccctgacc cagcggaccg
     2821 gtggtggttt ccacaccagc ggtataacct acttccggat ggcccggagt tttagagtgc
     2881 agctgacgga agtttttcag ttcttccatc ggcagatcgt aaccggtgag gtgcagcagg
     2941 ctgtagatca gcatggagcc gtggccgttg gacagcacga agcggtcacg gtcagcccag
     3001 gacggattct gcgggttgtg tttcaggaaa tcacgccaca ggacttcggc aatgtcagcc
     3061 atacccatag gggcacccgg gtgaccggat ttggctttct gtactgcgtc catgctcagc
     3121 gcacgaatag cattggcaag ctctttacgt gaggacattt tgactccaga tcggatgatg
     3181 aagggcacgc ccttaacgac ttgacgacag cgcgttttgg gctacgccgg aaaatttgcc
     3241 aacaatttac cgcaagccgc gcgtcatgta catggaacat ccttttgccg cttcagaaat
     3301 ctctggatca tgctcgcatg ttgcgcaatc tgctcgcccg tccgctgcgc ttttccttat
     3361 actgagactg agcgtcgatt cacctgcaaa cggcgcattt ttagaataat cctgaccttg
     3421 tgcggaagag aaaacatgaa aattcgcgcc ttattggtag caatgagcgt ggcaacggta
     3481 ctgaccggtt gccagaatat ggactccaac ggactgctct catcaggagc ggaagctttt
     3541 caggcttaca gtttgagtga tgcgcaggtg aaaaccctga gcgatcaggc atgtcaggag
     3601 atggacagca aggcgacgat tgcgccagcc aatagcgaat acgctaaacg tctgacaact
     3661 attgccaatg cgctaggcaa caatatcaac ggtcagccgg tgaattacaa agtgtatatg
     3721 gcgaaggatg tgaacgcctt tgcaatggct aacggctgta tccgcgtcta tagcgggctg
     3781 atggatatga tgacagataa cgaagtcgaa gcggtgatcg gtcacgagat ggggcacgtg
     3841 gcgttaggcc atgtgaagaa agggatgcag gtggcgcttg gtacaaatgc cgtgcgagta
     3901 gctgcggcct ctgcgggcgg gattgtcggc agtttatctc aatcacaact tggtgatctg
     3961 ggcgagaaat tagtcaattc gcaattctcc cagcgccagg aagcagaagc cgatgattat
     4021 tcttacgatc ttctgcacca acgcggcatc agcccggcag gtcttgccac cagctttgaa
     4081 aaactggcga agctggaaga aggtcgtcaa agctcaatgt ttgacgacca tcctgcatcc
     4141 gccgaacgtg cccagcatat tcgcgatcgc atcagcgcgg atgggattaa gtaaagccta
     4201 gtggtgtcga acgtaggtca gataaggcgt tcacgccgca tctgacatta atggcacatt
     4261 tgttaccttg tgcgcattgc cggatgcgat gctggcgcat cttatccggc ctacgggtgc
     4321 cgaacgtagg tcggataagg cgttcacgcc gcatccgaca ttaatggcac gttttacccg
     4381 tgcgcatcgc atctggtgct tactcgccct ttttcgccgc ctgaatatac agcatctcca
     4441 gcgccagcgt cgccgcagcc agagcggtga tttccgactg atcgtatgcc ggagccactt
     4501 ccactacgtc catcccaacg atgttgagat ctttcaggcc gcgtaccagt ttaatggcgc
     4561 gatcggaggt caggccgcca atcactggcg taccggttcc cggcgcgaaa g
//
LOCUS       DAJTYM010000182         4550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTYM010000182 DAJTYM010000000
VERSION     DAJTYM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            39..4157
                     /gene="pic"
                     /locus_tag="OO575_003854"
     CDS             39..4157
                     /gene="pic"
                     /locus_tag="OO575_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR6666623.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4308..>4550)
                     /locus_tag="OO575_003855"
     CDS             complement(4308..>4550)
                     /locus_tag="OO575_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR6666624.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1217 a         1035 c         1215 g         1083 t
ORIGIN      
        1 acttttatat cccttgtaaa catcatggag aatccatagt gaataaagtt tattctctta
       61 aatattgccc cgtcaccggg gggcttattg ctgtctctga acttgcccgc agggtaataa
      121 aaaagacatg ccgaagatta acgcatattc ttctggctgg cattccagca atctgtctgt
      181 gttactctca gatatcccag gcgggtattg tccgttccga tattgcctat cagatttatc
      241 gtgatttcgc cgaaaacaaa gggctttttg tacctggtgc caatgatatt ccggtatatg
      301 ataaggacgg aaaacttgtg ggaagactgg gtaaagcccc aatggccgat ttcagcagtg
      361 tgagctcaaa tggcgttgct acgcttgtat cgcctcagta tatcgtcagc gtaaagcata
      421 acggaggata tcggagtgtg agctttggta atgggaaaaa tacatattcc cttgttgacc
      481 gtaataacca cccttctatt gacttccatg ctccacgtct gaataaactg gttacagaag
      541 ttattccctc agcggtaaca tcagaaggaa ccaaagccaa tgcttataaa tacactgaac
      601 gttacaccgc tttttatcgg gtgggtagtg gtacgcagta cactaaggac aaggacggaa
      661 atttagttaa ggttgccggt ggatatgctt ttaaaacagg aggaaccaca ggagttcctc
      721 tgatatctga tgcaacaata gtctctaatc ccgggcaaac ttataatcct gtaaacggcc
      781 ctttacctga ctatggagcc cctggggata gtggttctcc tttgtttgct tatgataaac
      841 aacaaaaaaa atgggttatt gttgctgtat taagagcata tgcaggtatt aatggtgcta
      901 cgaactggtg gaatgtcata ccaacagatt atctgaacca ggttatgcag gacgatttcg
      961 atgcccccgt agactttgtt tccggactgg gccccctgaa ctggacatac gacaaaacat
     1021 caggcacagg taccctgagc cagggcagta aaaactggac catgcacggg cagaaagaca
     1081 atgacctcaa tgccggtaaa aatctggtat tcagcgggca gaatggtgca attatcctga
     1141 aagacagtgt gactcagggt gccggttatc tcgaatttaa agacagttac accgtatctg
     1201 ctgaatccgg aaaaacatgg acgggtgccg gcattattac tgacaagggg acgaatgtaa
     1261 cctggaaggt caacggcgtt gccggtgaca acttgcataa gctgggggaa ggaaccctga
     1321 ccataaacgg aacaggtgta aacccgggag gactgaaaac gggagacggt atcgttgtac
     1381 ttaaccagca ggcagacact gcaggtaata tccaggcctt cagttcagtg aacctcgcca
     1441 gcggacgtcc gaccgtggtg ctcggggatg cccgtcaggt caatccggat aacatttcat
     1501 ggggataccg gggaggtaag cttgacctta atggtaatgc cgttaccttc acccgactgc
     1561 aggctgctga ttacggggcg gtgattacaa ataatgcaca gcaaaaatcc cagcttttac
     1621 tggatcttaa ggctcaggat acaaatgtca gtgaaccgac gattggaaat atatccccct
     1681 ttggtggtac cggcactcca ggaaacctgt acagcatgat actcaacagc cagacccgct
     1741 tctatattct gaaatctgcc agctatggta acactctgtg ggggaacagc ctgaatgatc
     1801 cggctcagtg ggagtttgtt ggcatgaaca aaaacaaagc agttcagaca gtaaaagata
     1861 ggatcctggc cgggcgggca aaacaacccg ttatctttca tggtcagctg accgggaata
     1921 tggatgtcgc cattccacag gtgccggggg gaagaaaggt aatctttgat ggtagcgtga
     1981 acctgccgga aggtaccctg agtcaggaca gtggcaccct gatattccag ggacatccgg
     2041 ttatccatgc ctccatcagt ggcagtgcac cggtcagcct gaaccagaaa gactgggaaa
     2101 accgtcagtt tacaatgaaa acactgtcgc tgaaagacgc tgacttccat ctttcacgta
     2161 acgcctcgct gaacagtgac attaagtcgg ataacagcca tatcacactg ggaagtgaca
     2221 gggcatttgt ggataaaaat gacggaacag gaaattatgt cattccggag gaaggtacct
     2281 ctgtcccgga caccgtgaat gacaggagcc agtatgaagg gaatattacg ctgaaccata
     2341 actcagccct ggatatcggc agcaggttca ccggggggat tgacgcttat gacagtgccg
     2401 tcagcatcac ctctccggac gtcctgttga cagccccggg tgcttttgcc ggcagttcac
     2461 tgacagtgca tgatggcggt catcttacag cactgaacgg tcttttcagc gacgggcata
     2521 ttcaggccgg taagaacggc aaaatcaccc tgagcggtac accggttaaa gatacggcta
     2581 atcagtatgc ccctgctgta tatctgacgg acggatatga cctgaccggc gataacgcag
     2641 cactggaaat tacccgtgga gcacatgctt ccggtgatat tcatgcctct gcggcatcaa
     2701 cagttaccat cgggtctgac acgccggcag aactggcttc tgcggaaacg gctgcatcgg
     2761 cgtttgccgg cagtcttctt gagggctata acgcagcatt caatggtgcc ataaccggtg
     2821 gcagggctga tgtcagtatg cataatgcac tgtggactct gggtggggac tctgccatcc
     2881 acagtcttac cgtcagaaac agccgtatta gttctgaagg agaccgtaca ttccgtaccc
     2941 tgacggtgaa taaactggat gcaacaggca gtgattttgt tttgcgtacg gacctgaaaa
     3001 atgccgataa aattaatgtg actgaaaaag ccactggttc agataacagc ctgaacgtca
     3061 gctttatgaa taatcctgct cagggacagg ccctgaatat tcctctggtc acggcaccgg
     3121 cgggaacttc agcagagatg tttaaggccg gcacccgggt gacaggtttc agtcgggtga
     3181 ccccaaccct gcatgttgat accagtggtg gcaatacgaa gtggatactg gatggtttta
     3241 aagcggaggc tgataaagcc gctgccgcga aggctgacag tttcatgaat gccgggtata
     3301 aaaacttcat gacggaagtt aacaatctga acaaacgtat gggtgacctg cgtgacacaa
     3361 acggtgatgc cggtgcctgg gcgcgcatca tgagtggtgc cggttctgca gacggtggtt
     3421 acagtgataa ttacacccat gttcaggtcg gctttgacaa aaaacatgaa ctggacggtg
     3481 tggacctgtt taccggtgtc acgatgacct ataccgacag cagtgcagac agccatgcat
     3541 tcagcggaaa gacgaaatcg gtggggggcg gtctgtatgc ttcagcattg tttgagtccg
     3601 gtgcctatat cgatttgatt ggtaaatata ttcaccatga caatgattac acaggtaact
     3661 ttgctagcct gggaacgaaa cactacaaca cccattcctg gtatgccggt gctgaaacgg
     3721 gttaccgcta tcacctgaca gaggacacgt tcattgagcc gcaggctgaa ctggtttacg
     3781 gcgccgtgtc cgggaaaaca ttccgctgga aagacggtga tatggacctg agcatgaaga
     3841 acagggactt cagtccgctg gttggaagaa caggggttga actgggcaag accttcagtg
     3901 gtaaggactg gagtgtgacg gcccgtgccg gaaccagctg gcagtttgac ctgctgaata
     3961 atggagagac cgtactgcgt gatgcgtccg gggagaaacg gataaaagga gagaaggaca
     4021 gccggatgct gtttaatgtt ggtatgaatg cgcagataaa ggacaatatg cgctttggtc
     4081 tggagtttga gaagtcagcc tttggtaaat ataacgtgga taatgcggta aacgcgaatt
     4141 tccggtatat gttctgatgt gggggtaaag ggctgccgga aggcagcctt tctcagagac
     4201 tcttaaaata taaaactgaa ggtcttcggt aagggggagt gtattagacg tcaactagtt
     4261 ttgccgactg gcgcgagcct gtttaagatt ctgtgtaaat gcctttctca gaagtgaccg
     4321 tccaggcggt caccgaactc gataataaag cggctcattg ccattcgcca gtccttcaac
     4381 ggcatcgtcc atttctggga cgcagactgg attgccagcc acaccacttt tttcaccgag
     4441 tcgtctgtcg ggaacacttt acgctttttg atcgcatggc ggatcacgct gtttagcgac
     4501 tcgatggcat tcgtcgtata gatcactttg cggatgtccg ttggataagc
//
LOCUS       DAJTYM010000183         4436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTYM010000183 DAJTYM010000000
VERSION     DAJTYM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..3346)
                     /gene="narZ"
                     /locus_tag="OO575_003856"
                     /pseudo
     CDS             complement(1..3346)
                     /gene="narZ"
                     /locus_tag="OO575_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(3428..>4436)
                     /gene="narU"
                     /locus_tag="OO575_003857"
     CDS             complement(3428..>4436)
                     /gene="narU"
                     /locus_tag="OO575_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6666625.1"
                     /translation="FGIFIVIALLCGFAGANFASSMGNISFFFPKAKQGSALGINGGL
                     GNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAW
                     SGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVN
                     ILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNF
                     IAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQREAVTETAAT
                     LGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQ
                     K"
BASE COUNT         1029 a         1193 c         1206 g         1008 t
ORIGIN      
        1 tcggtccacc gcgagagagc gtcagcatta gcaggttttc actgtaggtt gagtgaatgc
       61 cccatttctg gtgcggcgtc aggaagttaa gtgctttttc ctggaagccg tttggcggta
      121 tctggcgcat ctcactgacg ctacgggtgt cgataggcgg gcgataagcc accaggcttt
      181 caccaaaagc acgcatccac ggatgatcct gatagagctg ctggcgtccc gacagcgtgc
      241 gccacggaat taactcatga acgttggtgt atcccgcgtt ataggatacg tgatcgcttt
      301 ccagaccgga ccatgtgggg ctggagataa ttttacgcgg ctgcgcctga atatcgcgaa
      361 agcgaatctt ctcgtcctct ttgtgcagcg ccagatgggt atgttcgcgc ccggtgatct
      421 cgcccagcgc ctgccacact ttgactgcaa catgaccgtt ggtttccggt gccagtgcca
      481 gaatcacttc tgaagcgtca atggcggtgt caatcagcgg acgcccctgt gccgggccat
      541 cacgcttggt gtaattgagt ttgccgagga aatcgatttc atcctgggta ttccacgaaa
      601 tccctttacc gccattgcca agtttgtcca tcaatggccc gagcgaggta aagcgttcat
      661 acgtagcagg gtagtcgcgc tccaccgcca caatattcgg cgcggtttta cccgggatca
      721 gatcgcattc gcctttgcgc cagtcgagca cttcacacgg ctgtgagagc tctgccggag
      781 agtcatgcag cagtggttgt aataccacgt cggtttcttt gccaagatgg cccacgcaca
      841 cttgcgaaaa tgctttggca atacctttgt agatttccca gtcgctgcgt gattcccagg
      901 ccggatcgac cgccgcagaa agcggatgaa taaatggatg catatccgag gtgttcatat
      961 cgtctttttc ataccaggtg gcggtgggca gaacgatatc ggagaacagg caggtactgg
     1021 acatgcggaa gtcgagcgtc accagcaggt cgagcttgcc ttcaatcgcc gcagtttgcc
     1081 actcgacttc ttccggtttg atcccgtcgc tggcaccgag ttcctcgccc tgaatcccgc
     1141 tttcggtccc cagcagatac ttctgtatat actcgtggcc tttgccggag gagtcaagca
     1201 ggttagagcg ccagacaaac aaattacgcg gatgattgct gccgctatct ggttgttcgc
     1261 aggccatacg taaatcgccc gatttcagcg cctgggcggt aaattctgtg ggggataatc
     1321 cggccttgtc ggcttcagct ttaatcccga gcgggttacg ccccagctgc ggcgcagaag
     1381 gtagccagcc catacgttcg gcgcgaacgt tgaaatcaat cagatgaccg ctgtacttac
     1441 tggcatcggc gagcggtgaa agtaactcct gagcagagac tttttcatag cgccattggc
     1501 tggaatgatt gtagaaaaac gaggtgctgt tcatctggcg cggtggtcgg ttccagtcga
     1561 gcgcaaaggc cagtggcaac cagccggttt gtgggcgcag tttttcctgg ccgacatagt
     1621 gtgcccagcc gccaccgctt tgcccgacac aaccgcagaa gatcagcata ttgatcatcc
     1681 cgcggtagtt catgtccatg tgataccagt ggttaacacc tgcgccgagg ataatcattg
     1741 agcgcccatg cgttttatgg gcggtatcgg caaattcgcg ggcgatggtt tcaatatact
     1801 ggcgcggtac gccggtaatt tgctcacccc aggctggggt gtacggtttg atttcagcgt
     1861 aatctttcgc gctgttttca tcttccagcc cgcgatcgag gccgtaattc gccagtacca
     1921 aatcataaac gctgaccacc ggacaggtgt tgccatcgac taacgtcagg tttttaacgg
     1981 gcaattggcg caccagcacc ggattgtgtt ttacgctgcg aaaatgcgga ttttcaatgc
     2041 cgccaaagta ggggaaggcc acgcctgcaa cagcgtcatg ttgaccgagc agggttaacg
     2101 acaattcggt ttccgtaccg gcggcaatgg attccagatt ccatttgcct ttttctcccc
     2161 agcggaaacc aatcgaaccg ttcggcacta ccaattcacc tgcggtatta actgctacgg
     2221 ttttccactg cggattgttg ctttcgccca gtccatccac caggtcagat gcgcggatca
     2281 tgcgcccggg aacgtagcta ccatcgtcgc gaggctccag cattaccagc atcggcatgt
     2341 cgctgtagcg gcggcagtag ttgataaagt agtcgctggg attatcgaga tgaaactctt
     2401 ttaaaatcac atgccccatt gccatcgcca gggcgctatc agtgccttgt ttcggtgcca
     2461 gccactggtc gcacaatttg gccacttcag agtagtcagg ggtaatggcg atggttttag
     2521 tgcctttgta gcgtacttcg gtaaagaagt gggcgtccgg cgtacgtgtc tgcggtacgt
     2581 tagaccccca agcgatgatg tagctggagt tataccagtc ggcagattcc ggtacgtcgg
     2641 tttgctcgcc ccaggtcatc ggcgaggcgg gcggcaggtc gcaataccag tcgtagaaac
     2701 tttaacaggt gccgccaagc agcgacagat aacgcgttcc ggcggcgtaa gaaaccatcg
     2761 acatcgccgg gatcggcgag aaaccggcaa cgcgatccgg gccgtaggtt ttgatggtcc
     2821 agacgttagc ggcggcaatc agctggttta gttcctgcca gttggagcgg ataaacccgc
     2881 cgcgcccacg cacttgtttg tagctcaggc acttttgcgg atcattcata atcgatgccc
     2941 acgccagtac cggatcgctg tgttgtttga gggcttcgcg ccacagttca atcagtcgtt
     3001 tacgaatgag cgggtatttc aggcggttag cgctgtaaag ataccaggag taacttgcgc
     3061 cacgcgggca gccgcgaggt tcatgattgg gcaggtcagg gcgagtgcgc gggtagtcgg
     3121 tctgttggat ttcccaggtc accagaccat ttttaacgta gattttccag ctacaggagc
     3181 ctgtacagtt aacgccgtgg gtggaacgca cgattttgtc gaactgccaa cgctggcgat
     3241 agctgtcctc ccagtcgcgg ttgctatgca tcacctgtcc gtgaccatcg gcaaaggttt
     3301 cgcccttttg tttgaagtag cgaaagcgat ccaaaagttt actcatgaca tgactcctgc
     3361 tccaggaatg aattgggatc tgatgccttg tcggatgcga catggttgta catcaggcat
     3421 cgatctctta tttttggctg aacttccgcc gaccataaac cagccaggtc agcagcacac
     3481 aaacgatgta gaagattaaa aacactttca tcgcgccgac cggagagccg gtcatattga
     3541 gcgacatgcc aaatgcctgc ggaataaaaa agccgcccac tgcgccaatg gctgagataa
     3601 agcccagcgt tgccgccgtt tcggtgacgg cttctctctg agcttgctca tcactaccgc
     3661 ctttcatctt cacccgataa atggttatct ggcgaaagat gacggcgatc atctggaaag
     3721 tagaaccact tcccagaccc gcggtcagaa acagccccat aaatacggcg taaaaggcga
     3781 tgaaattacc ggagcctgtg cccggtaagg taaggaacag cagggcactg aaaatcgcca
     3841 taaaaataaa gttgatcaac gtcacccgca cgccgccgaa tttatcggaa atagcaccac
     3901 caaccgaccg cgcgatggca ccaataaatg ggccaaagaa cgccaggcgc agaatattca
     3961 catccgggaa ctgggttttt gccagcatgg caaaacccgc agaaaaaccg ataaacgaac
     4021 cgaaggtggc aaggtaaagc aggctcagca gccagagatg caggcgttgt aagacaggga
     4081 gctggtcggc aattgaggcg cgtgaactgg cgatatcatt catccctgac caggcggcga
     4141 tcgtggcaat cgccagtagc ggtacccaaa tccatgcggc attcgccagc gacatcaccg
     4201 aaccgtcggc ctgcggtacg ccattgacgc cgagaaaggc aaatacaggt acaaaaatga
     4261 ccagcggtgc aaccagctgc attacactta cacctaagtt tcctaatccg ccattaatcc
     4321 caagagcgct cccttgtttg gcttttggaa agaagaaact gatattgccc atgctcgaag
     4381 caaagtttgc acctgcaaaa ccgcatagca aagcgataac gataaatatc ccaaaa
//
LOCUS       DAJTYM010000184         4228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTYM010000184 DAJTYM010000000
VERSION     DAJTYM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO575_003858"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO575_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6666626.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO575_003859"
     tRNA            complement(324..399)
                     /locus_tag="OO575_003859"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO575_003860"
     tRNA            complement(406..480)
                     /locus_tag="OO575_003860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO575_003861"
     tRNA            complement(597..681)
                     /locus_tag="OO575_003861"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO575_003862"
     tRNA            complement(690..765)
                     /locus_tag="OO575_003862"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO575_003863"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO575_003863"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6666627.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO575_003864"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO575_003864"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6666628.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO575_003865"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO575_003865"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6666629.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1092 a         1050 c          940 g         1146 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggcttt ctgcttaatt tgatgcct
//
LOCUS       DAJTYM010000185         4184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTYM010000185 DAJTYM010000000
VERSION     DAJTYM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            170..1078
                     /locus_tag="OO575_003866"
     CDS             170..1078
                     /locus_tag="OO575_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6666630.1"
                     /translation="MPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEA
                     TFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKM
                     TNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVR
                     IPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLT
                     QAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGD
                     YLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1078..1812
                     /locus_tag="OO575_003867"
     CDS             1078..1812
                     /locus_tag="OO575_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6666631.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2279..3040
                     /locus_tag="OO575_003868"
     CDS             2279..3040
                     /locus_tag="OO575_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6666632.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3084..4163
                     /locus_tag="OO575_003869"
     CDS             3084..4163
                     /locus_tag="OO575_003869"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6666633.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
BASE COUNT         1167 a          794 c         1028 g         1195 t
ORIGIN      
        1 tttagtcctt acacaaagcc gtgtcggcct tcttggttta ttttgcgtca tcgtgctggc
       61 cgctctcgcg gacagacccc gctggcgcgg acgtcccctt agcggtctgt tgcgcttgat
      121 cttcgtcgcc tccttgctgt gctggtgtgt cggtatctca aactatgcca tgccagggga
      181 gacaacggct gactttacgc attcatcatc gaatgaccag cgtatacgta ttctgaaagg
      241 tgccataagt cttattcaac agcgcccctt aaccggttgg gggttaggca gctttgaagc
      301 gaccttcccg caagccttag aggatgtggg cgtgacaaac atagagagtg atacggctac
      361 gcatccccat aacgaactgc tgttcgtaac ggctgaaggt ggcatcgtcg ccttgtttgg
      421 ttttttgctg ctatcaggcg tttggctgtg gccgtgtata tgccgcctga aaatgacaaa
      481 caataacgcg caccatcaat gtgaaaagtg gtttattgcc ttaatggggt tgcctttagt
      541 cattcatatg atgaccgaat atcctctcta tacgtctgtt ccccatttga tgctgttgct
      601 gctccttttc cgcatcggaa tgccggaaaa cgtgatgaat caggtacgta tacctaaaac
      661 agtccggttg ggtattctgc ctgtcattgt gctcattatg accgccgcgc tggtcatcct
      721 ggtagcggga tttgatgcac agcgggaact gaccagagct gaagctgata tgaacgaagg
      781 atttatccct cgccttccgg aacatagctg gaaatacctg acgcaggcag aacgtcttga
      841 gcgcgatcag catctgctta ttgcgaataa accggggttc gttcgcgata cggaaagcat
      901 gtctcttttc actgtgtggg gaaataaatg gttggcaatc cacaatgatg cagacgtaag
      961 cgctgcaatg atattcattg cgcacaggcg cggtgattat ctgacagctg aaaaaataag
     1021 aaaacgcgca gctcgggtat ttgtaaatga cgaacgtttt actcgcgggg gaaactaatg
     1081 gttaacgatc tgacgtataa gaaattagaa agcacaaaca tcaactgtaa tattctctcg
     1141 ccagaaaata ccacatggac aaatgtcacg gtgattggcc tggatctttt tgccatttgc
     1201 ggcgtgtgca gtttgctcaa gcgtgttggg ggagtgaaac ttcaggtttt cacttcctta
     1261 cccactgata tgaatgttct gactgacagt agcatcgtga tatgggtaaa aatgcgtcac
     1321 gatggtttgc ctgaattagc cggacatatt gtgaaggttt gtcgtcgata ccgtctgatg
     1381 cggcaactcg ttattagcga tgctattcct gaaggaatga cgaatgatat cgggcctctt
     1441 tcccgagtat ggcttactca tgggagtgca aaagtggata ttttattatc ttcattacgg
     1501 aaggtgttaa atgctcattg tttgcctggt cctttgttta taaaacgcct gggaagagtg
     1561 caatggaggg ttcttttact ccgcgcgaag gggattagca cacgtaaaat tgcagaaatc
     1621 tgtggtattg gcctcaagac cgttagcgcc catgagtccg caattcgaga acgattaggc
     1681 accaggaata aaagtgagta cgtctggtta gtacgtaatg ctcaacttat acaacaggcg
     1741 atacccgcgt tgggaagaga tgcgtcaagg tatgaaagat gtaacaatat gcgaacaaag
     1801 caaaaagtat aattgatgcg tcatagataa ctctcttttt cgatgacgaa atcgctgagc
     1861 atgcgtggtg aggttagtaa tagatgggtg ctatgttttg ccacgggttt gtagctgtga
     1921 aacctggaaa aaaataagaa ttaaaaatat ttaaagagag ttaataaggt tctggttgta
     1981 attgtcttgg tcaaaaaaaa gatattgtca atgttgtttt ttaaattttc aggttttggt
     2041 taaatatttg cttgcaaata taggtgattt tttattgtag gcttttgaag gctattataa
     2101 ctgtgcgcta aattaatgcg ttgaataaaa gaaataacaa aattaagaaa agccatattt
     2161 acgatatggg caacataaat ataatctaaa aaagagtaag gcgttgtagg ttggttccgc
     2221 gttcattacc ttgttagtga acgcggattt atcagttgtc gaaatggttt ttataataat
     2281 gaaaaatttt aatgtactaa ccaaagtcgt attttgcttg tcgtttttat caagcgcatt
     2341 tcccacctat gctgcggatg atgtcaaagt gactcaaaac acttcagttt ttaaactgaa
     2401 gcttagtgca acgcgattaa tctatgaaca agggtcggat ggcgcagcga taactgtgac
     2461 aaatccgcag gattggccga tgcttgtgca gagtaaagtc tttgaagaag ataaagtaac
     2521 tgcagcgccc tttattgtca ctccgccact gttcaggctt aatccaggcc agcaaaataa
     2581 aatgtgggtt gtgcagactg cgccagcttt acaagacagg gaaaaattac actggctatg
     2641 cgccaccggc attacgccta aaaagagtga tgtttgggcc gagaaagata aagaaaaaat
     2701 tgctgaaaat gtgaacctga acatccaggt taacgtacat acctgcataa aacttattac
     2761 caggccatct ggtattgaag gcaagatggt taatgcgagt gagaagttat cgtggaaaaa
     2821 gcagggtgat aaaattgtcg cggtcaatca gtcgccgttc tacatcaacc tttcttcttt
     2881 acaggttggc ggtagcaaga ttgagaaact agattatatt gcgccgtttt cgcaacatga
     2941 gtttgttatg ccaaaaacta gcggccagga tattaaatgg tctgtgatta ctgatgaagg
     3001 tggtgagagt cgtttgttta tatctcattt gcaatcataa tgtattacat ttttattgaa
     3061 tatttttatg ccatgcatat tccatgtttt atcgactgtc attaattttg ataatggcct
     3121 tacttgcggg tcagttaagt gcccaggagt ggtcatttga ttcttcacag ttggaaggaa
     3181 acgttagtgc ggacactgtg gcaatgttta atcagggaga gcaactcccc ggaaattatc
     3241 gtgtggaaat ttatcttaac ggtgaaaaag ttgatgttgg ggagtttccc tttcatcgtc
     3301 cggaatcccc tgaagaaaaa gagcttgttc catgtttaac cgttgatgac cttatccact
     3361 atggcattaa aattgataaa agcagcagtg atactgacaa taaaaagaat cagtgtttta
     3421 aatggaactc tattgaaggt ttgaaagtaa attatgattt tgatagccag cgcgtacaga
     3481 taaccgttcc acaactgtat ctgcaagata aaaaaagtag tttagcacca gtgtcgttat
     3541 ggaatgaggg tgtagctgca ttccgcatgg tgtatcagac aaatattgat atttccaaac
     3601 aaaatgataa tcaatctacc acgcgtaatt ctcgttatgg caggttcact ccagggttta
     3661 atcttggtgc ctggaggttt cgtagttcag tgacatggag caaggaactt ggccagtcag
     3721 aaagatggca aaggggttac atgtggtttg agcgaggcat aaatgcgatt aaaagccgat
     3781 taacgcttgg agaatcttat acgtcgtctg aggtgtttga cagcatacct tttcgcggcg
     3841 ggatgttagc gaccgacgat gcgatgactc cgccggagga cagctactac acacctgtgg
     3901 tgcatggaat agcacaaagt gaggcgcagg ttattattaa acaaaatggg caaattattt
     3961 ttacccgttc tgtgccgcca gggccttttg cgctggataa tttaccaacg ctggccgttg
     4021 gcggggagct tgatgttaca gtgagagaga gtaatggcga ggagcagtat ttttccgtgc
     4081 catttcaaac gccggccatc gctctgcatg aaggatattt caaatatagt gtgatggggg
     4141 ggaatataaa gaagaaggta taaaaagctc ccctgtctcc gtgg
//
LOCUS       DAJTYM010000186         4175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTYM010000186 DAJTYM010000000
VERSION     DAJTYM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(176..760)
                     /gene="kptA"
                     /locus_tag="OO575_003870"
     CDS             complement(176..760)
                     /gene="kptA"
                     /locus_tag="OO575_003870"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6666634.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1010..1693
                     /locus_tag="OO575_003871"
     CDS             1010..1693
                     /locus_tag="OO575_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6666635.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1690..2151
                     /locus_tag="OO575_003872"
     CDS             1690..2151
                     /locus_tag="OO575_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6666636.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2164..3336
                     /gene="iadA"
                     /locus_tag="OO575_003873"
     CDS             2164..3336
                     /gene="iadA"
                     /locus_tag="OO575_003873"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6666637.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3401..>4175
                     /gene="hypT"
                     /locus_tag="OO575_003874"
                     /gene_synonym="qseD"
     CDS             3401..>4175
                     /gene="hypT"
                     /locus_tag="OO575_003874"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6666638.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRS"
BASE COUNT          955 a         1112 c         1057 g         1051 t
ORIGIN      
        1 aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt aatttcagca
       61 gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa caaacgctac
      121 attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat tgaatttatt
      181 gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca accgttgatg
      241 tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc atttcctgtg
      301 ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt gccgttgctt
      361 catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt ttttttatct
      421 cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt ttttcggcaa
      481 aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac ctaccatcac
      541 tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc aatgcacggg
      601 ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc gcccatcctt
      661 cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa cttaaaaact
      721 tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg tccctggatt
      781 ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg caactcaatc
      841 ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac gcatagccag
      901 aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag ttccaggaat
      961 tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta tgacacaaca
     1021 aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag ggtatctggc
     1081 tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca gctggtggcg
     1141 cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg cgaatggagc
     1201 caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc tctttgcgct
     1261 ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg atggtcttgg
     1321 cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt tgggcattcc
     1381 agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg ccgccggtat
     1441 gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga ttttcgccgc
     1501 atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg tcgcggtgtt
     1561 tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt ttaaatttgt
     1621 cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc caacgcaagg
     1681 agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat cgatggcgcg
     1741 cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat ggcgtttgtc
     1801 atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat ctgtgaaccc
     1861 gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc cgcattaatg
     1921 agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc attaaccggc
     1981 cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt gcaaaatgtc
     2041 ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat cattacggtc
     2101 tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta acaaggagtt
     2161 accatgattg attataccgc agccggtttt accctgctgc agggagcgca tttgtatgcg
     2221 ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat cgccgttgcc
     2281 agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag cgggcagatc
     2341 ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg cgaagcaggt
     2401 cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg cgtcacgtca
     2461 gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct gctcgccaag
     2521 acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc ttatcatgtc
     2581 ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga tcgtgtgatt
     2641 ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta tcacctggct
     2701 aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg cgtcaccgtg
     2761 ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct ggaaaactgc
     2821 gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc gttgtttgag
     2881 caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag cattgacgaa
     2941 ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc gctggcacgc
     3001 gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga agggaattta
     3061 acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt gctggtcaaa
     3121 gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt agccggtttc
     3181 cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt actggtcatg
     3241 acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt caaagacggc
     3301 aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg ccctaatgga
     3361 cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact gtggtgcggt
     3421 tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa aatgccgcaa
     3481 tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc gccgcatccg
     3541 tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc cactgcaact
     3601 ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac agttagaaag
     3661 caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca agatagccgc
     3721 tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc cgccgctctt
     3781 tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc gtgaagggca
     3841 aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt ttgaccacat
     3901 tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg gagaagcact
     3961 ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact cctacatggt
     4021 gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca ccttttttgt
     4081 ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga ttgcctggct
     4141 gccggagtat gccatccaac aagaaattcg cagcg
//
LOCUS       DAJTYM010000187         4147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTYM010000187 DAJTYM010000000
VERSION     DAJTYM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(165..2114)
                     /locus_tag="OO575_003875"
     CDS             complement(165..2114)
                     /locus_tag="OO575_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6666639.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2199..2534)
                     /locus_tag="OO575_003876"
     CDS             complement(2199..2534)
                     /locus_tag="OO575_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6666640.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2534..2890)
                     /locus_tag="OO575_003877"
     CDS             complement(2534..2890)
                     /locus_tag="OO575_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6666641.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2890..>4147)
                     /locus_tag="OO575_003878"
     CDS             complement(2890..>4147)
                     /locus_tag="OO575_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6666642.1"
                     /translation="TDPFGELYVIAVPEARGAAATVRVTVAGEAEESGTLSLYVGRSR
                     VQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDAGVVTLTARHKGLYGNELPVCLN
                     YYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAAMGDEAFDFIGLPFNDAASINMM
                     MTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAGDMHNQQHITLAGYEKETQSPVD
                     ELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKGKRFIMTEQQTLLSHGVATAYVE
                     GGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAYVLRKLKSVITSKYGRHKLANDG
                     TRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYDLFKQYLIVERDADNPNRLNTLF
                     PPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT          959 a         1236 c          908 g         1044 t
ORIGIN      
        1 taataaatcg tgaaacaatc tcctcatttg ttagtatttt cgcctgagca cagcaagagg
       61 ttaatattgc aaccgtcatt aaaaatggct ttatcacaaa acaaactcca ttgttagcat
      121 gttacgcgat aattttgcct catctttcac cacaaaaaaa gaactcacct tttaaatgga
      181 gaatacccaa catcatgggt gattttcata aagggatcgc ctgttttcgg caaatcgata
      241 acgcgcattc caggcggcgc gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt
      301 gaagatgcaa ggcttaacag ctgattcctt tccagtgata cattcggggt gtagcgttta
      361 tcgggagata aatacccatc accacgtggc atctgccatc cggtcatgtc atagacaaaa
      421 tcatggaact gtttgcccca ctcgtcgaac ttatcattca gaccaaatcc actgttcagt
      481 gcatcagcca caaaattttt aacaagccag ggatgttctt tttcaaactc ctgcgccatc
      541 atgcccagtt caagcaatcc accaatcaga ctgatctttc cgaggccctt taatttcagc
      601 cctgtttttc ctgccgccgt tgtccattga ttctgggcga ctgttgccgc acgaatactt
      661 gccaccgcct tcacaccaat atacagggca gaaaccgttg ctattgtttt tactgcctgt
      721 tcccatcccc ccattgctct gacaacctga tcaacctcct gccagacttt tttcacaacc
      781 ggaccgaccg tttcccagtt atcaattatg agatatgcac caccgacgag aagcgcaatt
      841 aatccctttg ccggcgtcat attcatcacg ctacccatga tttttgttat gcgtgtcaat
      901 gtgccaatcg ccacgcccat cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg
      961 acctcaggat tttctctgac aaacgtgcgc acttcttcca gaaaaggctt catctctttt
     1021 atgccttcat tgagtgaagg caggaaggtt tcccccagcg tggaagaaat cgcattgatc
     1081 tggttctgca gcagcaacaa ctgattttct gtcgtcgcgg cacgggcggc atattctttc
     1141 tgcattgagc cgccatactg ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa
     1201 tccagattcg tgagcagcgg cgcaatggcc cccagggatt ccttcccgaa cagcgctttc
     1261 agcacagcag cctgtttttc tttcggcact tttgccagag aatccagtac gtgcagcatg
     1321 gccccgcggg cgtctttctg catatccgcc gccagttttt tcgggctaat gcgcaacgag
     1381 cggagcactt ttttctgcga tttggtggca gaatccctcg ctgtcagcga caacataaaa
     1441 ttctttatcc ccgtcgcagc aatttctgac tccaccccca ttccggcaat ggttgccccc
     1501 attgcggcaa tctctccgga ggccacaccc gcaacactgc ctaaagggcc aatacgggtc
     1561 acaacatcag aaatcttttt cgcacttgca ggaccggtat tgccaagata gttaatctta
     1621 tccgcaagcc ctgccacctc tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc
     1681 atctgaccgg attcttctgc cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca
     1741 gtaaactgca tcagctcgtc acgggcgata ccagcctgac cacccgccgc cacaatttcg
     1801 gcgatacctt ccgccgacat gggcagttct gttgacaggt cgcgcacctg ctccgtcatt
     1861 gccttaaacg cttccggcgt atccagacca tccaccactt tccggacatc cgccattttt
     1921 gattcaaggg caatggcaga tttgaccggg agcgccagcg cccccagtac tgcggttccg
     1981 gcacccgctg cgcccagtga caggctggca aactcctttt taaaaccttt cagctggcgc
     2041 tgcattccct tcagcggtgc cgatacctta tccacggcag tgatgatcgc cttcagctga
     2101 aaactgtcag ccatgcttca tctcctcgtt aatgcggacg gcctcggctt ccagttctgt
     2161 aaactgcgaa acagccaccc gtcgcagctc cagtggattc agtttccaga accaggcaac
     2221 attgtagagt cgtttccgga ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc
     2281 tgcatgctgg tcttaaaaat atccagtttt gccatctgcg ccgccgatga acacgggatc
     2341 cccgccagca acgggatata tttcagtgcc acctggctgt ccagtttaat actgccttca
     2401 ccggaaataa tgaaaggaaa acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac
     2461 tccagcacat gcagcatttc gttatgcgct gtcaccggtt tttttaaaac tatttctgta
     2521 acgttcattg ccattactga taccctccct cttcgccgtg gaattcaaga tctgccgtgc
     2581 cttcttcggc attatgatta gcctctccgt gcagccatgc cgccgaaagc acatacacca
     2641 tgccgtttgc cagctcagcg gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg
     2701 gaaagttttt gggcacttta aacgtgccct taacgtaagg tgaccgccag gtctccttgt
     2761 aatccacatc ccctgccatg ccgacgatgt catcccggac attggtgttc atcggcactt
     2821 caatgccacc tgtcagcgat aactgctgac catccacttt aaaaaaacag gttccggcga
     2881 tctttgccat tatgctgact cctctgaata ctgaagacgg aactggttaa ccaccgcaaa
     2941 gacacgtaac tgattaacat aatccggcgg gaacagcgta ttcagtcggt tcgggttatc
     3001 tgcatcacgc tcaactatca gatactgttt aaacagatcg tatttttcca caataccggc
     3061 acgctccatc tgacgatatg tcgccagaag ttcccctttg ataacggcag gagtgacaat
     3121 cgcctgcccc ggaccaaaac gggtaccatc atttgccagc ttgtgacgtc cgtacttgct
     3181 tgtgatgacc gatttcagtt tgcgaagaac atatgcactg gtatgcagag tttcactgtc
     3241 cagatagctg ttgtctgcca cgccatacgc attctttttg taggtggtta cggaacgctg
     3301 gatctgcaac gtgccgcctt ccacataagc cgtcgccacg ccgtgagata aaagggtctg
     3361 ctgctctgtc atgatgaatc gcttaccttt cggtgccgga agcatcccca ccagctcccc
     3421 cgtctgtgtc ggacgggcag gatcattccg gataaatacc gcttcacggg caaggcgact
     3481 ggcaaccagt tcatcgacag gcgactgggt ttctttttcg taaccggcaa gcgtgatatg
     3541 ttgctgatta tgcatatctc cggcaccaac cagctctgac agcgttccca gttttgcggt
     3601 atagacatgc ccgtataact ggcgcgcata actccagcga ccgctgctgt cattcatttc
     3661 ggtcatcatc atattgatgg aggcggcatc gttgaacggc agaccgataa agtcgaatgc
     3721 ctcatcgccc atagcagcaa cagcggcggt aagatcaggc gctccgctac cggcagcacc
     3781 gacttccgtc accacccgaa gccctgctgg caaaatctca ccaccaccag aaccataata
     3841 attcagacag accggtaact cattaccata cagcccctta tgacgggcag tcagcgtcac
     3901 cacgcctgca tcagatgacg ccgtaaacgg cagggtgata accccgttta ccgcttcctt
     3961 aatcgcggtg gcaaccgcag tggcatcatc gccattcacc acaggcacct gtacacggga
     4021 gcgcccgaca tacagactca gggtgccgct ttcctctgct tctccggcaa ccgtcaccct
     4081 gaccgtcgcc gccgcccctc tggcttccgg tactgcaata acatacagtt caccgaaagg
     4141 atctgtc
//
LOCUS       DAJTYM010000188         4147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTYM010000188 DAJTYM010000000
VERSION     DAJTYM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..120)
                     /locus_tag="OO575_003879"
     CDS             complement(<1..120)
                     /locus_tag="OO575_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666643.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGK"
     gene            complement(365..895)
                     /gene="cybB"
                     /locus_tag="OO575_003880"
     CDS             complement(365..895)
                     /gene="cybB"
                     /locus_tag="OO575_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6666644.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1084..2084
                     /gene="gap"
                     /locus_tag="OO575_003881"
                     /pseudo
     CDS             1084..2084
                     /gene="gap"
                     /locus_tag="OO575_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            complement(2126..3565)
                     /gene="aldA"
                     /locus_tag="OO575_003882"
     CDS             complement(2126..3565)
                     /gene="aldA"
                     /locus_tag="OO575_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6666645.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNVTKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3762..4100)
                     /locus_tag="OO575_003883"
     CDS             complement(3762..4100)
                     /locus_tag="OO575_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6666646.1"
                     /translation="MQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQ
                     LQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVL
                     IEAMESRSLR"
BASE COUNT         1100 a         1044 c          953 g         1050 t
ORIGIN      
        1 tttgccgcct gccgtacagt gtgagatccc ccctttcgaa ccagaacagg gtttgcgccc
       61 ggcatcggca taaaaggaaa caagcgttcc cataaacaaa ataagtgcca gttttttcat
      121 ataacctcca tggtttatcg aatcttatgt gcgatttgaa cgttatacgc tggtttattt
      181 tgataccgtt gatgagatca aagatcgggt gaattgatta aaaataagcc agatggacgt
      241 atgggcaagg attatggttt tatttgtcat acaaataaat ataataggcg cttccgatgt
      301 agacccgtat tcttcgcctg taccacgggt cggttttagt acaggcgttt tctttaaata
      361 tccttcagga acgtttacgc ggcatcatgc gtagaagtgt gttgtccttc cagaaatagt
      421 ggtgtgccag tgcagccgca gcgtgcaacc cgatgacaaa atatcccaga ttcgccagcg
      481 tctcatgcca cgactttaag ctatcaaccc gttcgaaatt ggcctctgaa gcgtaaggca
      541 tcgtcaaacc aaacgcaaac cacgggttgc cccggttata catcatcacc aaaccaatca
      601 ctggcagcgc aataaacagg agataaatca ccaaatgtcc caaatgcgcc agtcccgtca
      661 tcatcggttt cggcttaggt ataatcggcg gggttgggta tttcagtctt aacaaaagac
      721 gaaccaccat cagcacgaga attgagatgc cacaggaaac atgaatcatg ttgatgagcg
      781 gccgatcgct acgtgggaag aaaccacgaa actccattgc gcaatacgct gcgataacca
      841 gcaaaaagac cagccagtga atgctgattt gtaaccttga gtatttattt tccataacat
      901 ttcctgcttt aacataattt tccgttaaca taacgggctt ttctcaaaat ttcattaaat
      961 attgttcacc cgttttcagt acgttacgtt tcactgtaag aatggagatt gcccatccca
     1021 caatcctggt ctaagcctgg aaaggatcaa ttttcatccg aacgttcctg acaggagaaa
     1081 accatgagta aagttggtat taacggtttt ggtcgtatcg gtcgactggt gttgcgtcgt
     1141 ttacttgaag tcaaaagcaa catagacgtt gtcgctatta atgatcttac ttccccaaaa
     1201 attctcgcct atctgctgaa acatgattca aactatggtc cgttcccctg gagcgttgat
     1261 tttacggaag attcacttat cgttgatggg aaaagtatcg cggtttacgc cgaaaaagag
     1321 gctaaaaata ttccgtggaa agcgaaaggg gcagaaatca ttgtcgaatg tactggcttt
     1381 tatacctccg ccgataaatc gcaggcgcat cttgatgctg gtgcgaagaa ggtgttgatt
     1441 tccgctcctg ccggtgaaat gaaaaccatc gtttataacg ttaatgacga cactctggat
     1501 ggcaacgaca ccattgtttc cgtggcgtca tgcaccacta actgccttgc gccgatggcc
     1561 aaagccttgc atgacagttt cgggatagaa gtcggcacga tgacgaccat tcatgcctat
     1621 actggcaccc agtcactggt ggatggcccg cgtggtaaag atttacgtgc ttcacgcgca
     1681 gcggcagaaa atatcattcc ccacactacg ggggcggcaa aagccattgg tctggtgatc
     1741 ccggaactga gcggcaaact gaaaggtcat gcgcaacgcg tgccggtgaa aacaggttcg
     1801 gtcactgagc tggtgtccat tctcggaaaa aagtgactgc cgaagaggtg aataacgcac
     1861 ttaaacaagc aaccaccaat aacgagtcat ttggttatac cgatgaagaa atagtctctt
     1921 ccgatatcat tggcagccat ttcggttcag tgtttgatgc cacgcaaacg gaaattaccg
     1981 ccgtgggcga tttacaactg gtgaaaacgg tcgcctggta cgataacgaa tatggcttcg
     2041 tcacgcagct tattcgcacc ctcgaaaaat tcgctaaact ctgacgcgca caggcggagg
     2101 aaaaaacctc cgcctctttc actcattaag actgtaaata aaccacctgg gtctgcagat
     2161 attcatgcag gccatgttta ccatctgcgc cgccaatacc ggatttacgc catccggcgt
     2221 ggaaaccttg catagcttcg aagttttcac ggttgatgta agtttcacca aacttcagcc
     2281 ctttaatggc tttcatcgcg acgttcagat tttgggtata gattgatgag gtcaggccgt
     2341 aatcactgtc attagccatt gagatagcat cttccagcgt gtcaaatgcg acaactggca
     2401 gcaccgggcc aaaggtttcc tcatgcataa tcgacatttc ctggcgaaca tccagcagca
     2461 atgtcggcgg ataataatat cctttcccct ctaccgcttt gccacccaac gccactctcg
     2521 ccccttcttc tactgcgcgc gccacttttt gctcaaccct ttccagcgcc gcggcgttaa
     2581 tcaacggccc catcgcaatg tcgttgcgtt cagcggggtt accaaattga accgcctgca
     2641 acgcttcacc cagcagattg acgaactgat cataaatgcc tttctgtaca taaacacgtt
     2701 ctgcacagtt acacacttgc ccactattaa tgacgcgtga atcaacgatg gctttgactg
     2761 ccagttcaag atcggcatcg tccattacga tagccggtgc tttaccaccc aattccagac
     2821 acactttggt gacgtttttc gccgcagtcg ccatgatctt ctcacctgca gagacgctgc
     2881 ctgtcatact gaccattgcg acctttgggt tacccgccag ttcttgccca acggtttcac
     2941 cacgccccag tacaaggtta aacacgccgc gcggaaggcc tatttcatcg acgattttgg
     3001 cgaatgcaat cgcattgttt ggcgtaaatt cactgggttt aatgacgatg gtattaccgg
     3061 tcaaaagagc gggagccatt ttgcgggcaa tgaggaagaa cggaaagttc cacggcagaa
     3121 tgccggtagt cacaccaagc gcacgtttaa acagaagaat attttctccc ggacgatcgc
     3181 tttgaataat ctcgccctcg taacgccgtg cccactccgc catgtaatcg atatagtcgg
     3241 cagtaaaagc cacttcgact tcagctagct gctggatctt gcccccttct tcaacaatca
     3301 gcgcactgat ttcactggcg cgttcgcgga tcccggcgga gattttgcac aaccaactgg
     3361 cgcgttcaat agcaggcaac gcttcccatt ctggttgtgc acgttctgct gcatcgattg
     3421 ccttacgggc atcctcggcc tgaccatcgg gtatgcggga aatgacagcc tctgtagcag
     3481 ggtttaccac atcaatccat gcgtctccac gccaggtaac aaactgtcca tcgatataca
     3541 taggatgttg aacgggtact gacatgggcg actcttgtga tttatatgtt tgttttcggt
     3601 gaatatattg ttaattaaca tcaatcatca gagagacggc atcgggaaag gacaagtctg
     3661 tggagggctt cataaaaaca agtgtaaaag gaataaaagg cagaaataaa agtcaggagt
     3721 catgcccgtt tgcaaacaag tggttcacaa acgggcaatt tttaacgtaa cgagcgactt
     3781 tccatcgcct cgatgagcac cgcatcatgt ttcagcgttt gccatgcatg gatgacttct
     3841 gccggaaaat caacgtgaac gataaaatct cgcccgcggg gaccgtagcc atcgctatca
     3901 tcgtgtaaac gcggcagctc gccagtgagt agtgagagat aacgctcaac cggccataat
     3961 ccttgtaact gattaataaa gattacactg tctgcgttat ttcctaactg aggaacgaat
     4021 ccgggataac ttaaccagcg tggtgcatcg ggattgtccc cggtcatacg gcggaacgtt
     4081 gccatcgtgc gccgctgcat ggtggggtcc tgtacaacga tagccgtatg aactcgttct
     4141 acggcct
//
LOCUS       DAJTYM010000189         4114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTYM010000189 DAJTYM010000000
VERSION     DAJTYM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..158)
                     /locus_tag="OO575_003884"
     CDS             complement(<1..158)
                     /locus_tag="OO575_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6666647.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="OO575_003885"
     CDS             complement(308..469)
                     /locus_tag="OO575_003885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666648.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="OO575_003886"
     CDS             complement(466..654)
                     /locus_tag="OO575_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6666649.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="OO575_003887"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="OO575_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6666650.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="OO575_003888"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="OO575_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="OO575_003889"
     tRNA            complement(2161..2237)
                     /locus_tag="OO575_003889"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="OO575_003890"
     tRNA            complement(2339..2414)
                     /locus_tag="OO575_003890"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="OO575_003891"
     CDS             complement(2559..3380)
                     /locus_tag="OO575_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6666651.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="OO575_003892"
     CDS             complement(3395..3751)
                     /locus_tag="OO575_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6666652.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="OO575_003893"
     CDS             complement(3764..>4114)
                     /locus_tag="OO575_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6666653.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1117 c          794 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tcgggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAJTYM010000190         4052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTYM010000190 DAJTYM010000000
VERSION     DAJTYM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(896..1087)
                     /locus_tag="OO575_003894"
     CDS             complement(896..1087)
                     /locus_tag="OO575_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6666654.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1152..1475
                     /locus_tag="OO575_003895"
     CDS             1152..1475
                     /locus_tag="OO575_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6666655.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1417..2175
                     /locus_tag="OO575_003896"
                     /pseudo
     CDS             1417..2175
                     /locus_tag="OO575_003896"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2153..2683
                     /locus_tag="OO575_003897"
     CDS             2153..2683
                     /locus_tag="OO575_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6666656.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2690..2923
                     /locus_tag="OO575_003898"
     CDS             2690..2923
                     /locus_tag="OO575_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6666657.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3469..3861)
                     /locus_tag="OO575_003899"
     CDS             complement(3469..3861)
                     /locus_tag="OO575_003899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666658.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          968 a          922 c         1209 g          953 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacac ccgctgacaa gcccgtcaga
      901 ggttttcacc gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt
      961 cgtaaagcga ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca
     1021 gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1081 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1141 aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1201 gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg
     1261 cccgttccgg gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg
     1321 ggaataacct gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc
     1381 gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg
     1441 ccgtcaggga acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt
     1501 ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg
     1561 cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa
     1621 tacacgtccg gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa
     1681 gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt
     1741 ctgtgggtgg agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg
     1801 gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt
     1861 gatgcctggc agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag
     1921 aaccgccgga cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag
     1981 agcattacac ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat
     2041 gtgattcagg cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc
     2101 agcattgcag acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa
     2161 tcttttacag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga
     2221 gaaaatgctg agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc
     2281 aaacgagacg aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact
     2341 gaaaaaacat cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa
     2401 gctggcaggg aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag
     2461 tggcgggacg ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa
     2521 gcagaacgag agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg
     2581 gagcaacgga cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat
     2641 ggaaaacggg aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct
     2701 ggaaagccat ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa
     2761 cgaatgggag agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa
     2821 cgcgatgctg aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca
     2881 gacagaacgc ttgagccagt tatacagcga gaactggaga taagagagga acggttgata
     2941 caggagcgcg aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg
     3001 acactggatg gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg
     3061 tttccggtgc attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc
     3121 agaccttgct gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc
     3181 ttttgtcgct gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt
     3241 tcccgctcag gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg
     3301 tctctggctg gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa
     3361 tttaccgggt gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc
     3421 tgccgtgatc gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt
     3481 gtgagggaat ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga
     3541 ttgggtttcg gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa
     3601 ggcgtgagat tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct
     3661 tccgtccgat ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac
     3721 ttcttgaatt tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt
     3781 aggggagtgt attattctct cttttaataa gctgtttacc acagggttta gttgttgtct
     3841 aaagcacgca gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg
     3901 ggtatgccgt gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc
     3961 aacaaatacg gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct
     4021 tttttgcggc gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTYM010000191         4026 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTYM010000191 DAJTYM010000000
VERSION     DAJTYM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..335
                     /locus_tag="OO575_003900"
     CDS             <1..335
                     /locus_tag="OO575_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6666659.1"
                     /translation="QAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAAGSIF
                     TFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYLAFVK
                     RLGAFDAL"
     gene            325..1119
                     /locus_tag="OO575_003901"
     CDS             325..1119
                     /locus_tag="OO575_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6666660.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            1141..2565
                     /gene="patD"
                     /locus_tag="OO575_003902"
     CDS             1141..2565
                     /gene="patD"
                     /locus_tag="OO575_003902"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6666661.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            2775..3584
                     /locus_tag="OO575_003903"
     CDS             2775..3584
                     /locus_tag="OO575_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6666662.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
BASE COUNT         1034 a          822 c         1068 g         1102 t
ORIGIN      
        1 ttcaggcggc gcttgagcgt ttgccgccgt cattgttgca ggcgtcggct gatctcggcg
       61 cacgtccacg acaaaccttt cgctatgtgg tgctgccgct ggcaatcccg ggtattgccg
      121 ctggctctat ctttaccttc tcactcacac tgggcgattt tatcgtcccg cagctggttg
      181 gtcctccagg atattttatc ggcaatatgg tttattccca gcagggggcg attggcaata
      241 tgccgatggc ggcggcattc accctggtgc cgattattct catcgcactg tacctggcgt
      301 tcgtgaaacg tctgggagcg ttcgatgcac tctgaacgcg caccgttttt cctcaaactg
      361 gcggcctggg gcggcgttgt tttcctacat tttcccatcc tgataatcgc cgcctatgcg
      421 tttaacactg aagatgcggc gtttagtttt ccaccgcagg gcctgacgct gcgctggttt
      481 agcgtggcag cacagcgtag tgatattctt gatgccgtga cgctgtcact taaagtggcg
      541 gcgctggcga cattaattgc gctggtgtta gggacgctgg cagctgccgc gctgtggaga
      601 cgagactttt tcggcaaaaa cgccatttcg ctgttattgc tgctgcccat tgcgctgccg
      661 ggcattgtca ctggtctggc gctattaacc gcctttaaaa ccatcaatct ggagccggga
      721 tttttcacca tcgtggtcgg tcatgcgact ttttgcgtag tggtggtgtt taacaatgtc
      781 atcgcccgtt ttcgccgcac ctcgtggagt ctggttgagg cgtcaatgga tcttggggcc
      841 aatggctggc aaaccttccg ctacgtagtg ttgccgaatc tcagttcggc gttactggca
      901 ggaggaatgc tggcgtttgc cttgtcgttc gatgaaatca tcgttacgac ctttacggca
      961 ggtcatgaac gaacgttacc gttgtggttg ctcaatcagc ttgggcgacc gcgtgatgta
     1021 ccggtaacca acgtggtggc actgctggtt atgttggtaa caaccttgcc gatcctgggg
     1081 gcctggtggc taacccgcga aggcgacaat ggtcaataac cactgataca ggaataagct
     1141 atgcaacata agttactgat taacggagaa cgggttagcg gcgaagggga aaaacagcct
     1201 gtctataatc cggcaacggg ggacgtttta ctggagattg ccgaggtatc cgcagagcag
     1261 gtcgatgctg ctgtgcgtgc ggcagatgca gcatttgctg aatgggggca aaccacaccg
     1321 aaagcgcgtg cggaatgcct gctgaaactg gcggatgtta tcgaagaaaa tggtcaggtt
     1381 tttgccgaac tggaatcccg taattgtggc aaaccgctgc atagtgcgtt caatgatgaa
     1441 atcccggcga ttgtcgatgt ttttcgcttt ttcgcgggtg cggcgcgctg tctgaatggt
     1501 ctggcggcag gtgaatatct tgaaggtcat acttcgatga tccgtcgcga tccgttgggg
     1561 gtcgtggctt ctatcgcacc gtggaattat ccgctgatga tggccgcgtg gaaacttgct
     1621 ccggcgctgg cggcagggaa ctgcgtagtg cttaaaccat cagaaattac cccgctgacc
     1681 gcgttgaagt tggcagagct ggcgaaagat atcttcccgg caggcgtgat taacatactg
     1741 tttggcagag gcaaaacggt gggtgatccg ctgaccggtc atcccaaagt gcggatggtg
     1801 tcgctgacgg gctctatcgc caccggcgag cacatcatca gccataccgc gtcgtccatt
     1861 aagcgtactc atatggaact tggtggcaaa gcgccagtga ttgtttttga tgatgcggat
     1921 attgaagcag tggtcgaagg tgtacgtaca tttggctatt acaatgctgg acaggattgt
     1981 actgcggctt gtcggatcta cgcgcaaaaa ggcatttacg atacgctggt ggaaaaactg
     2041 ggtgctgcgg tggcaacgtt aaaatctggt gcgccggatg acgagtctac ggagcttgga
     2101 cctttaagct cgctggcgca tctcgaacgc gtcagcaagg cagtagaaga ggcgaaagcg
     2161 acagggcaca tcaaagtgat cactggcggt gaaaagcgca agggtaatgg ctattactat
     2221 gcgccgacgc tgctggctgg cgcattacag gacgatgcca tcgtgcaaaa agaggtattt
     2281 ggtccagtag tgagtgttac gcccttcgac aacgaagaac aggtggtgaa ctgggcgaat
     2341 gacagccagt acggacttgc atcttcggta tggacgaaag atgtgggcag ggcgcatcgc
     2401 gtcagcgcac ggctgcaata tggttgtacc tgggtcaata cccatttcat gctggtaagt
     2461 gaaatgccgc acggtgggca gaaactttct ggttacggca aggatatgtc actttatggg
     2521 ctggaggatt acaccgtcgt ccgccacgtc atggttaaac attaagttag atgttagtgg
     2581 ctgttatagg acggccacta tcgttttagg ttctgagttt aatggcggtt aagtattaga
     2641 atgattacat taataactgg cgtaaaaatt aggctaaatg tttgatggcg atttttcatc
     2701 ctgtcacagt tgattagcat aaggctttag agtatcgtta ttaaactaat actttataag
     2761 tagaagtatt aacaatgaaa gttcaattgt acaaattcac ggaagataaa aataaaaccc
     2821 ttaccttcag atggacgaaa aaacactttg agttttgtat ggataataaa atctttttaa
     2881 atcataaggg gaagaagtcg tataaagaaa ggaatttatt tcttttctca aaaggtgaca
     2941 agataacaat tgaagataat gttattgctg aagaatattc aacaatgcct gtgaaaaatt
     3001 tctccagtgt cggcgctttt tcatttccga cctgccattt ttcagggaat atacgtatag
     3061 gtcgattttg tagtattgca tcaaatgtaa aaataatggg tggaaatcat cctttgaatc
     3121 gttttactac tcacatgatg acttataacg gtgaatttga taagtttgct atgagtgaat
     3181 ttgaacgtag ctggacattg aaaccattca ttaccaaacc tgaaaatccg attataggaa
     3241 atgatgtctg gataggaaat gatgttgtat tgaaaggtgg catcgcaata ggtgatggtg
     3301 ctgttattgc tgccaattcc gttgttacca aagatgtgcc cccatatgcc atagttgcag
     3361 gagttcctgc aaaaataatc agatttaggt ttgattcaaa tgttattgat gaactgttga
     3421 gaataaaatg gtggaattat aactactcag atcttccgga taataataag tgtgatgata
     3481 taaattattt tgttgaagag atgaatcgat tgatatcaaa tggaaatatt caagagaggg
     3541 actataaaaa attcaatctt tctgaggtat tcagaggcct ataattatct gttttgcatg
     3601 ataaatgggc aagataacca gggcggggtt tgcaatttca atggggcagc gtcatcaagg
     3661 aggttgcaaa catcctgcca tgcatatact ttacgccatc tttttatatt tacgttattc
     3721 cttgaaagta taaagcattt atagactccc gcgcgtataa agtattcgtg atagaaaaat
     3781 gtgaaaacaa gattccgttt gaatcagtaa gctgtttgta tagacgacag tagggggatg
     3841 ttttctactc atttcaatgt agatgccagc aaaaagaagt aaatattaag gataatgttg
     3901 caaacttgga gagtatgaga agatcttgcg tactgtcaga gagttgattt ctctgacagt
     3961 acgtgctgcg tgcaaaatca ctacatcaaa taaaccagcc aaatctcata gagataagag
     4021 taaaca
//
LOCUS       DAJTYM010000192         3987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTYM010000192 DAJTYM010000000
VERSION     DAJTYM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..252
                     /locus_tag="OO575_003904"
     CDS             <1..252
                     /locus_tag="OO575_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR6666663.1"
                     /translation="QWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            274..2265
                     /gene="cyoB"
                     /locus_tag="OO575_003905"
                     /pseudo
     CDS             274..2265
                     /gene="cyoB"
                     /locus_tag="OO575_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2255..2869
                     /locus_tag="OO575_003906"
     CDS             2255..2869
                     /locus_tag="OO575_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6666664.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            2869..3198
                     /locus_tag="OO575_003907"
     CDS             2869..3198
                     /locus_tag="OO575_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6666665.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3210..>3987
                     /gene="cyoE"
                     /locus_tag="OO575_003908"
     CDS             3210..>3987
                     /gene="cyoE"
                     /locus_tag="OO575_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6666666.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRI"
BASE COUNT          814 a         1002 c         1095 g         1076 t
ORIGIN      
        1 caatgggtcg caaaagcgaa acagtcgccg aacaccatgt ctgacatggc tgcgttcgaa
       61 aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac
      121 ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag
      181 ccagaaggtg agcacagtgc acacgaaggt atggaaggca tggacatgag ccacgcggaa
      241 tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca
      301 gtcccgttcc ataaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg
      361 gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg
      421 acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg
      481 ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggt
      541 gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt
      601 atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg
      661 cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttact
      721 gttgttggcg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc
      781 tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg tgttgattac
      841 tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc
      901 gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatacc agtatttacc
      961 taggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc
     1021 gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt
     1081 ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc
     1141 ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt
     1201 ctgtttggtt atacctcgct ggtatgggca accgtctgca tcaccgtgct gtcgttcatc
     1261 gtttggctgc accacttctt tacgatgggt gcgggcgcga acgtaaacgc cttctttggt
     1321 atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc
     1381 atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggactatcgg ttttatcgtc
     1441 accttctcgg tgggcggtat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt
     1501 ctgcataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc
     1561 ttcggctgct tcgcagggat gacctactgg tggcctaaag cgttcggttt caaactgaac
     1621 gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg
     1681 ccgctgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg
     1741 cagttccaca ccatgctgat gattgcagcc agcggtgcag tactgattgc gctgggtatt
     1801 ctctgcctcg ttattcagat gtacgtttct attcgcgacc gcgaccagaa ccgtgacctg
     1861 actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc
     1921 tataactttg ccgtagtgcc gcacgttcac gaacgtgatg cattctggga aatgaaagag
     1981 aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc
     2041 ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat gatctggcat
     2101 atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc
     2161 ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag
     2221 catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc
     2281 gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt
     2341 ttggatctac ctgatgagcg actgcattct gttctcaatc ttgtttgcta cctatgccgt
     2401 tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt
     2461 tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc
     2521 catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg
     2581 tgccggattt atcgggatgg aaatctatga attccatcac ctgattgtta acggcatggg
     2641 gccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca
     2701 cgtcacttct ggtcttatct ggatggcggt gctgatggtg caaatcgccc gtcgcggcct
     2761 gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt
     2821 ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct
     2881 actgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg
     2941 tcgatcattc tgacggtgat tccgttctgg atggtgatga caggggctgc ctctccggcc
     3001 gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc
     3061 ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc
     3121 gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac
     3181 aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga
     3241 aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa
     3301 agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg
     3361 cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga
     3421 cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca
     3481 ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct
     3541 ggctgggggt gatgggcttt gtggtttatg tcggcgtcta tagcctgtac atgaaacgcc
     3601 actcagtcta cggcacattg attggttcgc tctccggcgc tgcgccgtcg gtgattggct
     3661 actgtgcagt aaccggcgag tttgatagcg gcgcagcgat cctgctggct atcttcagcc
     3721 tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg
     3781 caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc
     3841 tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctgggt
     3901 ataaatatct ggtggtcgcc gcggcggtta gcgtctggtg gttaggtatg gctctgcgcg
     3961 gttataaagt tgctgatgac agaatct
//
LOCUS       DAJTYM010000193         3845 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTYM010000193 DAJTYM010000000
VERSION     DAJTYM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..375)
                     /gene="azoR"
                     /locus_tag="OO575_003909"
     CDS             complement(<1..375)
                     /gene="azoR"
                     /locus_tag="OO575_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6666667.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENG"
     gene            576..>3845
                     /gene="hrpA"
                     /locus_tag="OO575_003910"
     CDS             576..>3845
                     /gene="hrpA"
                     /locus_tag="OO575_003910"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6666668.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LP"
BASE COUNT          954 a          991 c         1050 g          850 t
ORIGIN      
        1 accgttctcg gtatagcgga aagtaacgcc tgcgcgtgca accaggtcaa aatagttttt
       61 caactgagtt gagatgttga agttatacat cggtgccgca ataacgataa cgtcgtgggc
      121 tttcagctcg gcaatcagct catcggaaag cgccagagct tcctgctgac gcggagtcag
      181 cggcgcatcg ctcggacgca gagcgccaac cagttcgcca tccagtaccg gaatcggatt
      241 tgcagctagg tcgcgaacgg tgatttcatc agcggagtgc ttttcgctcc attgttcaac
      301 aaaataatcg gacaactgat tagactgaga gtaccctgcc aggatgctgg atttaagaac
      361 taataccttg ctcatggtgt ttccttatag atgtttgaat ggacgatgcc ccgttgcttg
      421 ctgacacttt attcacaatc ctgccacaga gatcgcgcaa taaatcgaag tctatgttcg
      481 aatttattga acaacgcata gaaagccgcg atgtggtact ctatatctat catttaaaag
      541 aaaattaatc aggcagacta ctgcccacta acgttatgac agaacaacaa aaattgacct
      601 ttactgcctt gcaacagcgg ctggattcgc tgatgctgcg cgacagactg cgtttttctc
      661 gccgtctgca ttgtgttaag aaggttaaaa atcctgatgc acaacaggcc attttccagg
      721 agatggcgaa agagattgac caggcggcag ggaaagtcct gctgcgtgaa gcggcacgac
      781 cggaaatcac ttatcctgac aatttaccgg ttagtcagaa aaaacaggac atactcgaag
      841 cgattcgtga tcaccaggtg gtgatcgtcg ccggggaaac cggctccggt aaaacgactc
      901 agctaccgaa aatctgtatg gagctggggc gcggtattaa agggctgatc ggtcataccc
      961 agccgcgtcg cctggcggca cgaacggtgg ccaaccgtat tgcggaagag ctgaaaacgg
     1021 agccgggcgg ttgcatcggt tacaaagtgc gtttcagcga tcacgtaagt gataacacga
     1081 tggtcaagct gatgaccgac gggatcctgc tggcggagat ccagcaagac cgcctgctga
     1141 tgcagtacga caccatcatt attgacgaag cgcacgaacg cagcctgaat atcgattttc
     1201 tgctcggcta tttgaaagag ttgctgccgc ggcgtcctga cctaaaaatc attatcactt
     1261 ccgcgactat cgacccggaa cgcttttcgc gccactttaa taatgcgccg attattgaag
     1321 tctccggtcg gacctatccg gtggaagtgc gctatcgccc gattgttgaa gaagccgatg
     1381 acaccgagcg cgatcagttg caggcgattt ttgacgccgt agacgaacta agccaggaaa
     1441 gccccggcga cattctgatc tttatgagcg gcgagcggga aattcgcgat accgccgatg
     1501 cgctgaacaa gctgaactta cgccataccg aaatcttgcc gctttatgcg cggctttcga
     1561 acagcgaaca gaaccgcgtg ttccagtcgc acagcggacg gcgcattgtg ctggcgacca
     1621 acgtcgcgga aacctcgctg accgtaccgg ggattaaata cgtcatcgac cccggtacag
     1681 cgcgtatcag ccgctacagc tatcgcacca aagtgcagcg tttgccgatt gaacccattt
     1741 cgcaggcgtc tgccaatcag cgtaaaggcc gctgtggtcg tgtgtccgaa gggatctgta
     1801 ttcgtctcta ttccgaagac gatttcctct cgcgcccgga gtttaccgat ccggagattc
     1861 tgcgtaccaa cctggcctcg gttattttgc agatgaccgc gctggggctg ggcgatatcg
     1921 ctgcgttccc gtttgtcgaa gcaccggata aacgcaatat ccaggatggc gtgcgtctgc
     1981 tcgaagaact gggcgcgatc accacggatg aacaggccag cgcctataaa ctgacgccgc
     2041 tcggtcgcca gctctcgcag ttgcctgtcg acccacgtct ggcgcgtatg gtgctggaag
     2101 cgcaaaaaca tggctgcgtg cgtgaggcga tgattatcac gtccgcgctc tccatccagg
     2161 atccgcgcga acgtccgatg gacaaacagc aggcatcgga cgaaaaacat cgtcgcttcc
     2221 acgacaaaga atccgacttc ctcgcgtttg taaatctgtg gaattatctt ggcgagcagc
     2281 aaaaggcgct ttcttccaac gccttccgtc gcctgtgtcg taccgattac ctcaactatc
     2341 tgcgcgtgcg cgaatggcag gatatctaca cccagttgcg ccaggtggtg aaagaacttg
     2401 gcattccggt caacagcgaa ccggcggagt atcgtgaaat tcacatcgcc ttactgaccg
     2461 gtttgctttc ccatatcggc atgaaagatg ccgataaaca ggaatatacc ggtgcacgta
     2521 acgcgcgttt ctccatcttc cccggttctg gtttattcaa aaagccgccg aaatgggtaa
     2581 tggtggcgga actggtagaa accagccgcc tgtgggggcg cattgctgcg cgtatcgacc
     2641 cggaatgggt agaacccgtt gctcagcatt tgattaaacg cacctacagc gaaccgcact
     2701 gggaacgggc gcagggcgcg gtgatggcaa cggaaaaagt cactgtttat ggtttgccga
     2761 ttgttgctgc gcgcaaggtc aactacagcc agatcgattc ggcgttatgt cgtgaactct
     2821 ttattcgcca cgctctggtg gaaggtgact ggcagacgcg tcacgcattc ttccgtgaaa
     2881 acctgaaact gcgggccgaa gtggaagagc tggaacacaa atcacgtcgc cgcgatattc
     2941 tggttgatga cgaaacgctg tttgagttct acgaccagcg catcagccac gatgtaatct
     3001 ccgctcgtca cttcgatagc tggtggaaaa aagtcagccg cgaaacgcct gatttgctca
     3061 actttgaaaa gagcatgttg atcaaagaag gggcggaaaa aatcagcaag ctggattacc
     3121 cgaacttctg gcatcagggc aatctcaagc tgcgtttgag atatcagttt gagccaggcg
     3181 cggacgcgga cggtgtgacc gtacatattc cgctgccgtt acttaaccag gttgaggaaa
     3241 gcgggtttga atggcagatc cccggtctgc gccgcgaatt ggtgattgct ctgattaaat
     3301 cgttgccgaa accggtacgc cgtaattttg tgcctgcacc aaactacgcc gaagcgtttt
     3361 taggccgcgt cacaccgctg gagttaccgt tgctcgacag ccttgagcgc gagttacggc
     3421 ggatgaccgg cgttaccgtt gaccgcgaag actggcactg ggatcaggtg cccgatcacc
     3481 tgaaaatcac cttccgcgtg gtggatgaca aaaacaagaa gctaaaagaa gggcgctcgc
     3541 tacaagatct gaaagatgcg ctgaaaggca aagtgcagga aacgctgtcc gcggtggcgg
     3601 atgacggtat cgagcagagc ggcttacata tctggagttt tggtcagctg ccggaaagtt
     3661 acgaacagaa gcgtggcaac tataaagtga aggcgtggcc ggcgctggtg gatgaacgcg
     3721 acagcgtgac gatcaaactg tttgataacc cgctggagca aaaacaggca atgtggaatg
     3781 gtcttcgccg tctactgctg ctaaatattt cctcgccgat caagtattta catgagaagt
     3841 tacca
//
LOCUS       DAJTYM010000194         3826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTYM010000194 DAJTYM010000000
VERSION     DAJTYM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            240..2672
                     /locus_tag="OO575_003911"
     CDS             240..2672
                     /locus_tag="OO575_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6666669.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..>3826
                     /locus_tag="OO575_003912"
     CDS             2739..>3826
                     /locus_tag="OO575_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HCR6666670.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKT"
BASE COUNT         1104 a          972 c          897 g          853 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaa
//
LOCUS       DAJTYM010000195         3823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTYM010000195 DAJTYM010000000
VERSION     DAJTYM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..224
                     /locus_tag="OO575_003913"
     CDS             <1..224
                     /locus_tag="OO575_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6666671.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OO575_003914"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OO575_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6666672.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OO575_003915"
     CDS             518..1108
                     /locus_tag="OO575_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6666673.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OO575_003916"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OO575_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6666674.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OO575_003917"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OO575_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6666675.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OO575_003918"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OO575_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6666676.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="OO575_003919"
     CDS             3457..3648
                     /locus_tag="OO575_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666677.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1048 a          928 c          954 g          893 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctccatat gaacggaacc
     3781 gacgattaaa ctggatggct ctgattgatt cagggtatga atg
//
LOCUS       DAJTYM010000196         3790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTYM010000196 DAJTYM010000000
VERSION     DAJTYM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..2576)
                     /locus_tag="OO575_003920"
     CDS             complement(<1..2576)
                     /locus_tag="OO575_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6666678.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WL"
     gene            2784..3773
                     /gene="ldhA"
                     /locus_tag="OO575_003921"
     CDS             2784..3773
                     /gene="ldhA"
                     /locus_tag="OO575_003921"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6666679.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
BASE COUNT          948 a         1004 c          910 g          928 t
ORIGIN      
        1 tccaaccatg cctggagatt atcgccaaag cgtaaactgc gccacagcgt aaaaatattc
       61 tcttcatgat ggtaattaag gtttaccgta ccgcttttac catcaacgcg actggtacct
      121 gtaatgttgg cctgcatggt taataaaccg agattatcta agttaatttt tgtcgatgac
      181 cggctaattt ccatatagcg caaccagtta attgctgaac ccgcagtcat attgtctttc
      241 accaccgcat ctgcggtatc tttatccagc cgcaaggtca ttagcccgct atttgccagc
      301 caaccatctt tcactatcca tttttcgttg tttagccata acggcaatgc accactaaat
      361 gccccggaca tggcgaattg tttcggattg acggcgctaa ccagttcgct ggatgagaga
      421 ttattcagcc gcaacagcgc cgggtcatgt tgcggcatac gtaattgttt catcagcacg
      481 ttaccgccta acacatcgac gctaacatca gtcaacagca agggttcttc ttcagtccac
      541 ggataacgcc cttgcaaatc agccgtaata tttttcgctg tcaccagatt aatcacttcg
      601 gcaattcgca acgtaacggg gccgcgagtc cccagatgcc aggctccatc ggcaaaacgg
      661 aaaggcagga caaaatcgac accgttaacc tgattatctg gcatccaggc actaccgcct
      721 ttcaacacgc cgtgccctcc cgcgcggaat ccttgttcag gcgcagcaga aaatgcaacc
      781 tgagcataca gttcaccatc gcgtaagttc atcttccagt cgggtggcac cagcggctgg
      841 aatacagtca gtgattgttt tggccaccag gcgttaccgc gcagacgaat accgtcccag
      901 cgaccattta cccgaaccgg gccaatctca ccagcatgta aatcgccttt aaagaggaaa
      961 taggtcggat tgcgcccatc gacgctaaat tttaaggttg atggcggtaa cacactgccg
     1021 ccagtgaaca gcgtttgccc ggcgtccagt gacagcgcgc cgctgaagga gggatgctgt
     1081 gcgtcacgta cccagacgac gggcttgtcg agaattaatc gcggcttttc taccgtcatc
     1141 gtaccgtatt gtaactggtc gaaaccggtg gagagatcgg tcagcgtaat cgtgctgtca
     1201 tgccactcac cttttcctgc gacatcccag gtggcattca tcggtgtaaa actccctttt
     1261 ccccagtagc gccactgcca gcggccagcg tcagggagaa aatcattcgc cagcccatcc
     1321 atatgcagca cgaaatcgcc cagttcattt tcatgcgcct gcaaaatggc ctgcaaacgt
     1381 ccgtcaacac cacgttgggt gacttttaca cccgctaaag gccagcggat ttcatcgatg
     1441 tccagcgaat cgatgactct tccctttgaa cgaagtaacg cgcctggctc aaaggtcagc
     1501 gttgggtcag acagacttcc acttagctgc gcaggtaaac gggcatataa aatgaggtcc
     1561 gcctgtttcg cttcaccggt cagctgcaga ggcagttgac tgttatccat gcttaatttt
     1621 cctgggccaa aattaagcac cgcgttgccc ttacccgctt gcccctgggt cagcacattc
     1681 agtcgtccgc tgaccagagc gttctcaagc cctgcctgcc agttgtcgac tttgacaccc
     1741 agtcggccac tcaaaggaaa accagcatac agccagctcc agcgaccatc gcttacggtc
     1801 aattgttgac gagtaatttg ccacggcaaa tcgagcaacg gatcgccgtt atcccgtgcc
     1861 agcacgatca attgcccgct attttcctgc cagtccagct cggcatccac cagtgacggt
     1921 tcctgcggca agtttaacgt cgcagtagca tgcccactta caggaagtcc atccggcacg
     1981 agcggcatag taaattcccc caccagttta accggcggct gattttcaaa cgcgacgaca
     2041 tccagttcgc tgactgtaag ttgttgccct ttcagctggc cttgaaattt aactttttcg
     2101 ccctgataac gcagttgctg gatatcagag gttaatgcga gagagagttt tccctgccat
     2161 tcctgccagg gagaaaaaat cagtttatcg atattgatcc aggtgttagg cagcatggac
     2221 tgccactgcg cgagggtttt tggagcagct ggcgattgct ccgtctgcgg caatttcgcc
     2281 agacaagcag aatcaagttc taccgtgccg acgttcaata accagcggct gggatgtgaa
     2341 aggctggcgt tggtgatatg cgcaagctga caatctccca ccagataacg gagatcggga
     2401 atgattaaac ctttacgcgt aatgcgtggg ctttcatcta atgcaatacg tgtcccgagc
     2461 ggtagccaga tgtccgccag cgtgggaacc cacagcccga gcgtcatcag cagcgtcaac
     2521 ggcacaagaa taatcagtaa taacagcgcg agaacggctt tatatttacc cagcatgggt
     2581 agttaatatc ctgatttagc gaaaaattaa gcattcaata cgggtattgt ggcatgttta
     2641 accgttcagt tgaaggttgc gcctacacta agcatagttg ttgatgaatt tttcaatatc
     2701 gccatggctt tcaattatat ttgaaatttt gtaaaatatt tttagtagct taaatgtgat
     2761 tcaacatcac tggagaaagt cttatgaaac tcgccgttta tagcacaaaa cagtacgaca
     2821 agaagtacct gcaacaggtg aacgagtcct ttggctttga gctggaattt tttgactttc
     2881 tgctgacgga aaaaaccgct aaaactgcca atggctgcga agcggtatgt attttcgtaa
     2941 acgatgacgg cagccacccg gtgctggaag agctgaaaaa gcacggcgtt aaatatatcg
     3001 ccctgcgctg tgccggtttc aataacgtcg accttgatgc ggcaaaagaa ctggggctga
     3061 aagtagtccg tgttccagcc tatgatccag aggccgttgc tgaacacgcc atcggtatga
     3121 tgatgacgct gaaccgccgt attcaccgcg cgtatcagcg tacccgtgac gctaacttct
     3181 ctctggaagg tctgaccggc tttactatgt atggcaaaac ggcaggcgtt atcggtaccg
     3241 gtaaaatcgg tgtggcgatg ctgcgcattc tgaaaggttt tggtatgcgt ctgctggcgt
     3301 tcgatccgta tccaagtgca gcggcgctgg aactcggtgt ggagtatgtc gatctgccaa
     3361 ccctgttctc tgaatcagac gttatctctc tgcactgccc gctgacaccg gaaaactacc
     3421 atctgttgaa cgaagccgcc ttcgatcaga tgaaaaatgg cgtgatgatc gtcaatacca
     3481 gtcgcggtgc attgattgat tctcaggcgg caattgaagc gctgaaaaat cagaaaattg
     3541 gttcgttggg tatggacgtc tatgagaacg aacgcgatct gttctttgaa gataaatcca
     3601 acgacgtgat ccaggatgac gtattccgtc gcctgtctgc ctgccacaac gtgctgttta
     3661 ccgggcacca ggcattcctg acagcagaag ctctgaccag tatttctcag actacgctgc
     3721 aaaacttaag caatctggaa aaaggtgaaa cctgcccgaa cgaactggtt taatcttgca
     3781 gctcccctgc
//
LOCUS       DAJTYM010000197         3708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTYM010000197 DAJTYM010000000
VERSION     DAJTYM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            339..1991
                     /locus_tag="OO575_003922"
     CDS             339..1991
                     /locus_tag="OO575_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6666680.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2002..2172)
                     /locus_tag="OO575_003923"
                     /pseudo
     CDS             complement(2002..2172)
                     /locus_tag="OO575_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2305..2424)
                     /locus_tag="OO575_003924"
                     /pseudo
     CDS             complement(2305..2424)
                     /locus_tag="OO575_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2498..3428)
                     /locus_tag="OO575_003925"
                     /pseudo
     CDS             complement(2498..3428)
                     /locus_tag="OO575_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1023 a          856 c          848 g          981 t
ORIGIN      
        1 tcacagataa aacattctcc aggaaacttg gggcggttca cgtcagtatc agaccatatt
       61 aattgctgaa actgataaac atcaaacctt atagaagggt gaatgctgaa agggggctat
      121 ggtgatcgac gtgatgacaa cgacaggtaa gaccgtgacc tgataaacct gaattgtgtc
      181 ctagacataa agtccgtgaa gggaataagg ctcaggtcag cgaatcttat agtggcccgc
      241 agctctgact gcaataaggc cggatacaga gcagcaacta ctcgtcgatg tcagaacaac
      301 ggaagccttg caaacgggga tgcgttcata caggaacaat gataccgtta aaaaaaaaca
      361 ttacgctaat aatgttcacc ctgtcattac tgacaggaaa tcctgctata gcgtatgaaa
      421 cagataaagt atataaaata accgttctgc atacaaatga ccaccatgga cacttttggc
      481 gtaacaatca tggtgagtac gggttatcct cccaaaaaac tctggtggac aatatccggc
      541 agaaagtaat aaataatggc ggaagtgttc tattattatc tggtggtgat attaacaccg
      601 gtgtacctga atcagacctt cagaaggctg aacccgatat tcgtggaatg aatctaattg
      661 gttatgatgc aatggccgtc ggtaatcatg aatttgataa tcctctaaat atattacgtc
      721 agcaggaaaa atgggccaca tttcctttcc tttctgctaa catatatcaa aaaagtacag
      781 ggaggcgttt attcagtcca tggaaaattt tcatccgtca gaatttgaaa attgcagtca
      841 ttggcttgac gacagatgat actgcgaaaa caggaaattc tgaatatttt actgatattg
      901 agttccgtca acctgctgct gaggcgagga gtgtcattga tgaacttaat cagcaggaaa
      961 agccagatat tattattgcg gcaacacata tgggtcacta tgacaatggc gagtcaggtt
     1021 cgaatgctcc tggtgatgtt gaaatggctc gcagtttgcc gactggctca ttagcaatga
     1081 ttgtgggagg acattcacaa gctcctgtct gcatggccag cgataataaa aaacagtgga
     1141 attatattcc ggggacaacc tgtgtaccag ataaacaaaa tggcatctgg attgtacaag
     1201 cgcatgagtg gggaaaatat gtcggtcagg ctgattttga attctgtaac ggtacaatga
     1261 aactggttaa ttatcagtta cacccagtta atctcaaaat gagaattacc agagaagatg
     1321 gaaaaacgga attttctttt tacacccccg aaattactga agatccacag atgttatcct
     1381 tactgacacc attccagaat aaaggcaaag cacaactgga tgtgaaggtt ggggttgtga
     1441 acggacgtct ggaaggggac agaagtaaag ttcgttttgt tcagaccagt atgggccatc
     1501 tgattctgag cgctctgaca gaacgtattg acgcagattt tgctgttgtc agtggtgggg
     1561 aaattcgaga ttccatagag agtggtaaca tcacatacaa agatatactg aaagttcagc
     1621 catttggtaa tacggtagta agtattgatt tgacggggaa agaagtagct gactacctag
     1681 ctacagtggc acagatgaaa ccggattccg gagcctaccc acaatttttg aataccagct
     1741 ttgtagtcaa aaaaggaaaa atcgaaatgc taaaaataaa aggtaaatcg gttgacctta
     1801 acaaaaaata tcgtatgaca acatttagct ttaatgctac tggtggggat ggctatccac
     1861 gtattgataa tagacccgga tacataaata ccggatttat tgatgctgaa gtacttatcg
     1921 aatatatccg gaaacattca ccgctggatg ctgcttcata tgaaccaaaa ggtgaagtca
     1981 gttggcagtg ataacgtaat tgacgatcat cacgtgtttt ttgctgacag tcgctgtgac
     2041 tttgtgaaag tagtctctgc gtatgtttgc gatatgggaa tgcagttgct gtattttgtg
     2101 tttctgcttc tgccagttgt tgctgaattt aaccttgcgg cttaactgat gttggagcgc
     2161 tgccagcttc ttcgtcaatt tcgctgatta aagtttattc attatggcat gatctgcgta
     2221 agcggctcgt cagaaccata ttgatattta ctaagagctc agatcaactt tccagggcaa
     2281 cagatcgcgt acccggtttg ccggtcagtc ctggatatgc tcaatgacgt aacgcagcca
     2341 tttttctggc tccacattgt tcagacggca tgtgccgatc agcgagtaca acaccgccgc
     2401 atgttcgcca ccgctatcgg aaccccgccg gagagagttt cactctccgg ctgctcacgg
     2461 aaccgtacgt gacactctcg cgtcatacgg ctcttgatca ctcattcgat gcacgtagta
     2521 aataccagtg ggcaaatagc ttcggatact gtttccttac cagcgccagc tttcgctgag
     2581 ccttccggtt cgaaagtctg tatttagact gcatccactt aagcaaacgt gactgcattg
     2641 cactccacag tcgatagctg aagtttctgg accagaactt tccatagtac tcaatccagc
     2701 ctctcactat cgcattgtag cgcctcgcaa aatccagcaa actctcagct gttgagcgat
     2761 gtatacgcca cttcttgatt gtttctgtta ttttgcacat tgctgcattt gatgcacccg
     2821 gcatacattt tctgtacaat tcgcctttga aattcttcag agtacgcact ttgaagtcat
     2881 atccgagagt ggcatacaac atcatcggcg tacctttcga agggaacgcc gggaaactga
     2941 cgttgcatcc acatatcaaa ggcatagtgc aggaacagat ttgagagcag gggggagatt
     3001 acgccgccct gcggcgttcc tcgaccccgt gccagcattg ctccatccgg catttgcacc
     3061 ggtgccttca gcctgcgctc aatgtacatc accacccagc gtgcctgagt atgatgctgc
     3121 actgtcttca gtagcaggtc atgatcgata tcatcaacga agcctttaat atcgacttcc
     3181 accccccagt caaacttcca gcagcgtttc ctcgcctgcg caatcgcctg gtgtgcggat
     3241 ttacccggtc gataaccata cgagtctgca tggaacaggg gttccagtac cggctcgagt
     3301 atctgcttaa cgaccatctg ggcgatccgg tccgacacgg ccggaatacc gagcggacgt
     3361 acaccaccgt cagctttggg aatgtccaca cgccttaccg cctgcggcat ataactgccg
     3421 gaagccattc tgttccacag tttgtagaga ttgtctgtaa cattctcgtc aaacccggcc
     3481 agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt ttactcgttt ataagcttcc
     3541 catacctgtc gtttacttac aggaaatggc tttgccgatt ttacagcagt cctcccatcg
     3601 gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat ggggcaagcc ctttgctccg
     3661 gacccattac ggtccttcac cgctactacg gtttactccg tccctgta
//
LOCUS       DAJTYM010000198         3648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTYM010000198 DAJTYM010000000
VERSION     DAJTYM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(53..256)
                     /locus_tag="OO575_003926"
     CDS             complement(53..256)
                     /locus_tag="OO575_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666681.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(258..413)
                     /locus_tag="OO575_003927"
     CDS             complement(258..413)
                     /locus_tag="OO575_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6666682.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(616..1023)
                     /locus_tag="OO575_003928"
     CDS             complement(616..1023)
                     /locus_tag="OO575_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6666683.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1100..1327
                     /locus_tag="OO575_003929"
     CDS             1100..1327
                     /locus_tag="OO575_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6666684.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1311..1733
                     /locus_tag="OO575_003930"
     CDS             1311..1733
                     /locus_tag="OO575_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666685.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1811..2588
                     /locus_tag="OO575_003931"
                     /pseudo
     CDS             1811..2588
                     /locus_tag="OO575_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2595..3341
                     /locus_tag="OO575_003932"
                     /pseudo
     CDS             2595..3341
                     /locus_tag="OO575_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3356..>3648
                     /locus_tag="OO575_003933"
     CDS             3356..>3648
                     /locus_tag="OO575_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6666686.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWS"
BASE COUNT          922 a          800 c         1007 g          919 t
ORIGIN      
        1 cgggtatcgc gcgtctgttt ttgtttgcga attcagtcaa tgcgcatggt atttataccc
       61 cgccaagacc cggtgaatat cctgcacggc cttctgcttt gtagaaggct tcttcgctat
      121 cataatcgcc agattccagc gcttctttag ccagccagat gcgtgcccca gtgcctgcat
      181 ttggctccag ttgctggagg cgttttgcat cttctaggag tagagcgata acgtgtttta
      241 attctgtctc gttcatttta ctcacctgaa tgtcttccca accaacgacg tgcgccagct
      301 tcggttttaa acgttttgct tttggtatac gttatggcgg tgaatgtgcc gtcctggttg
      361 gggaacacgc cgtacaccgg agattcgttg ttgccaagat cgatagtatt catgttgacc
      421 ccatttcccc ttaacgccgg ggtagcggaa ctgtttgctg agaacaccgt gcggtgtctt
      481 gatgaataaa atttagaata acctaatgag ggtgatcaag atttttgtgt agaaaaacct
      541 aagttttttg atgtaaaaaa cacaagtgtt tgaaagtttg tgctttttat tactgggtgt
      601 ggagaaaaaa ggggattatt tgtttgcgct tcttttgcga gctttgagta gttcttcaaa
      661 aagtttgttg aaattttcaa ctcgagcacg catctctgac aacagggcct tttgctctga
      721 ctcaggcagt gcgtcgaaca gttgaagtaa ctctttttga tcttctgtca gaatggctgg
      781 ctgattatcc gggatcggtt cgcctggttg tttatcttca tccccaaaaa gaagccaagt
      841 cggtgagcac tgaagcgctt ggctcagtgc gaataatctt ttccccgccg gctgtgtttc
      901 atctctttcc cattgagaaa ttgttacgtg agccactttg accagcttac ctaatgcggc
      961 ctgagacagt tttaattttt tacgcctatg taagaggcga gcaccgaagg tttcgttttt
     1021 catattaggg aattctaatt tttcttgact taggtttctc tacggcctgg tttccttagg
     1081 aaaatctaag gagctcgata tgttgaaaat tgatgctata gcgttttttg gcagcaaaac
     1141 aaagcttgcc aatgccgcag gagttaggct ggcaagcatt gctgcatggg gggaactggt
     1201 tcctgaaggt cgcgcgatgc gcctgcaaga ggcatctggc ggggaacttc agtacgaccc
     1261 caaagtttat gacgaatatc gtaaggcaaa acgacctggg aaggtgattc atgaaaatca
     1321 ggcatgagca catcgaatca gtgctgttag ccctggcagc cgaaaaaggg caggcgtggg
     1381 tcgctaacgc aattactgaa gaatatctgc ggcagggggg cggcgaattg tctctggtac
     1441 caggcaagga ctggaacaat cagcagaata tctatcaccg ttggttgaaa ggtgaaacga
     1501 aagcgcaaag ggaaaaaatt cagaaactga tccctgcggt tctggcaatt cttccgcgtg
     1561 agctgcgtca ccgactctgt atcttcgata ccctggaacg ccgtgcatta ctggcggcgc
     1621 aggaagcgtt gagtacggca attgatgcgc atgatgatgc tgtccaggcc gtttaccgga
     1681 aagcacattt cagcggtggt gggtcgcccg gcgattctgt cgtagtgcat tgattgaaat
     1741 taatcgtgcc ggattgtttt gttcggtatc agttaaatgt aacgctgcga gcgttacaag
     1801 gtgaaaacaa atggcttcaa actggataaa gctcgaggtt attacgccgg ataagccgga
     1861 aatattcagg cttgctgaga ttctgaatat tgatccagat gccgcattag ggaaggttat
     1921 tcgcttctgg gcatgggcgg atcaacaaat gatagacggt aatgcagatt gtaacgctcg
     1981 cggcgttaca aaaagtgcaa tagatcgcat cacttttatg tctggttttg ctgatgcgtt
     2041 aattcaggtt ggatggctgg tcgaaaatga cggtgggctt tctctaccta actttgaacg
     2101 tcataacgga aaaagctcta aaaaacgggc ggttacaaac gagcgagtaa caaaaatacg
     2161 cgaactgaaa cgaaaaggta acgctgccag cgttacacaa acggatcaaa aagcgttacc
     2221 agtggaagag gaagaggaag atctaaatac tgatctcccc ctaaatcccc ctcgccaaaa
     2281 acgagcgtct aaaaaattcg agccggaggc tattgagctg cccgattggt tgccggaaac
     2341 actctggcat gagtgggtcc ggttcagaca ggcattgcga aaaccgattc gaacggagca
     2401 gggcgatacg ggaactggaa aaattccgtc agcagggttt tacacctgag caggtgattc
     2461 gacacagcat cgccaatgaa taccagggcc tgttcgcgcc gaaaggtgtt cggcctgaga
     2521 cgttgctccg acaggttaac accgtctcgt tgccggacag tgcgatcccg ccaggcttca
     2581 gggggtaacg gaccatgaaa aatattgcga caggcggcgt tctggaacgc atccgccgac
     2641 tgaccccgca acatgtaacc gccccattca gaacggtagc ggagtggcgc gagtggcaac
     2701 ttgctgaagg ccagaaacgt agcgaggaga tcaaccgcct gaatcgccag ttgcgggtgg
     2761 aaaaaattct gaatcgctca ggcatccagc cgttgcaccg taaatgctcg tttgcgaatt
     2821 accaggtgca gaacgacggc cagcgatacg cgttaagcca ggcgaaatcc atcgccgatg
     2881 aactgatgac cgggtgtaca aattttgcgt tcagcggaaa acctgatacc gggtagaatc
     2941 acttagcggc agctatcggg aatcgcctgc tgaaagacgg tcagacagtg attgtggtta
     3001 ccgtggctga tgttatgagc gccctgcacg ccagctatga cgacgggcag tcaggcgaaa
     3061 aatttttgcg ggagctgtgc gaagtggatc tgctggttct tgatgaaatt ggcattcagc
     3121 gcgagacgaa aaacgagcag gtggtgctgc accagattgt tgatcgccgg acagcgtcga
     3181 tgcgcagcgt ggggatgctg acaaacctga actatgaggt gatgaaaaca ttgctcggcg
     3241 agcgggtgat ggatcgcatg gtcatgaacg gcgggcgctg ggttaatttt aactgggaga
     3301 gctggcgtcc gaatgttagc cattcgaggg ttgttaagta gtttcaggag gatttatggc
     3361 gaaacctttt actcccgaac agcgggaaga actgaagacg cgaattgtgg aactcgtgca
     3421 tcaggacggt cgggtcacga ttcggcagtt gtccgatgaa acaggtatca gtcgtgcgtc
     3481 tgtcggtcgc ttatgcatag aactggtcgc aagtggtgat gtatataatt ctggctacgg
     3541 cttattcccg tctgaacagg ctcgcaagga ctggcaaagc gcccgcaaaa aactctcgag
     3601 agtaaaggtg aggaaaccgg ttgttgttga tccggacctt atctggtc
//
LOCUS       DAJTYM010000199         3505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTYM010000199 DAJTYM010000000
VERSION     DAJTYM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(385..1080)
                     /locus_tag="OO575_003934"
     CDS             complement(385..1080)
                     /locus_tag="OO575_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6666687.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1073..2500)
                     /locus_tag="OO575_003935"
     CDS             complement(1073..2500)
                     /locus_tag="OO575_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6666688.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2511..3230)
                     /locus_tag="OO575_003936"
     CDS             complement(2511..3230)
                     /locus_tag="OO575_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6666689.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          881 a          967 c          734 g          923 t
ORIGIN      
        1 tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg ctctgggatt
       61 ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg tagatgaata
      121 ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg tataaagctg
      181 aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg
      241 taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata accatgacgg
      301 aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc ttcttcaggt
      361 ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca ccgctttcac
      421 cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc tgggaccact
      481 gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat gcaatttttc
      541 tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa gagaagattc
      601 cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca cgcctcccga
      661 ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg cctgcgagat
      721 attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct gcaaacgttc
      781 actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact gatctcccag
      841 ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca gctcacagcg
      901 cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct gttggttaag
      961 ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt gcggttcaag
     1021 tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc gattatccat
     1081 tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct ccgtcagctt
     1141 caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt ggtgttttgc
     1201 cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact ttccacaatc
     1261 ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct tttgcggtga
     1321 tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc ggaatacgca
     1381 ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa tctttaaaat
     1441 ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga taaatagagc
     1501 catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa gccccgcagc
     1561 gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc ccgttatcga
     1621 caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc ggccctgtca
     1681 gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc atggtgatca
     1741 atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact gcaatatgcg
     1801 ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg cataccgaac
     1861 cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg aggaaatctt
     1921 cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc ccctcatagc
     1981 ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt gccgggacca
     2041 caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga tcggtttcaa
     2101 tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg gtcaccatcg
     2161 atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg
     2221 tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc actttttctg
     2281 agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc tgggcggccc
     2341 gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga tttgccccaa
     2401 tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt tgctgacgga
     2461 tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc tcagcggctc
     2521 atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg taatcgccca
     2581 ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg gacttccatc
     2641 aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt gaccgagaac
     2701 gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc
     2761 acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc caggctacaa
     2821 gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac ccctaattta
     2881 ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc
     2941 cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt gcaagagcca
     3001 gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt tttcatccct
     3061 ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca tccctgtttc
     3121 tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc tcgtgccatt
     3181 cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac aactaatact
     3241 ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat caatcatcaa
     3301 taaatatggc gttctttttt aattggctag caacatacca cccctcattt gtatgacaaa
     3361 tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt cggcatcgct
     3421 cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt aaaaatggct
     3481 acatccctta tacctattca gagct
//
LOCUS       DAJTYM010000200         3489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTYM010000200 DAJTYM010000000
VERSION     DAJTYM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..389
                     /locus_tag="OO575_003937"
     CDS             <1..389
                     /locus_tag="OO575_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6666690.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO575_003938"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO575_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6666691.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO575_003939"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO575_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6666692.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO575_003940"
     CDS             complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO575_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6666693.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3116..3487
                     /locus_tag="OO575_003941"
     CDS             3116..3487
                     /locus_tag="OO575_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6666694.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          949 a          641 c          716 g         1183 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa tcaagataat
     2701 gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt
     2761 aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt
     2821 taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg
     2881 aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat
     2941 aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac
     3001 ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt
     3061 aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca
     3121 attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat
     3181 tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac
     3241 caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat
     3301 tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct
     3361 tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta
     3421 catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggcaat
     3481 gcactgaga
//
LOCUS       DAJTYM010000201         3471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTYM010000201 DAJTYM010000000
VERSION     DAJTYM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..601)
                     /gene="ychE"
                     /locus_tag="OO575_003942"
     CDS             complement(<1..601)
                     /gene="ychE"
                     /locus_tag="OO575_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6666695.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIE"
     gene            1078..>3471
                     /gene="adhE"
                     /locus_tag="OO575_003943"
                     /gene_synonym="adhC"
     CDS             1078..>3471
                     /gene="adhE"
                     /locus_tag="OO575_003943"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6666696.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     "
BASE COUNT          936 a          880 c          805 g          850 t
ORIGIN      
        1 attcaatccc caatgccatc agcaatagcc ccataatacg cgtaatcacg ttgatgccgg
       61 tctggcgtaa aacccgtacc agccacggtg ccatgcggaa caatccccag caacataaag
      121 cgaacaatgc aatagccaca aagaaaccaa acagatagct aatgctgtga taacgcgtac
      181 cccagacgat ggtagaactg atcgcccctg gccccgccat caacggcaac gccaatggca
      241 ccacaccaat actttcacgt accgcggttt ctgatttttc ttgcttgttc tgtttatcct
      301 cgccaagctt gccgctgatc atcgacatcg ctatcgtcac caccaggatg cccccggcga
      361 tacggaacga atcaattgat ataccaaaaa gttgtagaat cgtgtcgccg agaaaaagcg
      421 agatccacaa gataatggcc acagacaggt tggctgtaag gttagttttg tttcgcgctg
      481 ccgctgtctg ataactggtc atgctgataa agacgggaat aatccctacc gggttgacca
      541 gcgcaaataa cccgatgaaa aatttgaagt aaacgggaaa atcaaaaagg gtctgaatca
      601 cggttcgctc cgaagcaaaa gccggataat gttagccata aataaggttg aaaagacgcg
      661 ctgacaatac gccttttgac agcatttttc acctcctaac tacttaaaat tgctatcatt
      721 cgttattgtt atctagttgt gcaaaacatg ctaatgtagc caccaaatca tactacaatt
      781 tattaactgt tagctataat ggcgaaaagc gatgctgaaa ggtgtcagct ttgcaaaaat
      841 ttgatttgga tcacgtaatc agtacccaga agtgagtaat cttgcttacg ccacctggaa
      901 gtgacgcatt agagataata actctaatgt ttaaactctt ttagtaaatc acagtgagtg
      961 tgagcgcgag taagcttttg attttcatcg gttaagcaaa tcatcaccgc actgactata
     1021 ctctcgtatt cgagcagatg atttactaaa aaagtttaac attatcagga gagcattatg
     1081 gctgttacta atgtcgctga acttaacgca ctcgtagagc gtgtaaaaaa agcccagcgt
     1141 gaatatgcca gtttcactca agagcaagta gacaaaatct tccgcgccgc cgctctggct
     1201 gctgcagatg ctcgaatccc actcgcgaaa atggccgttg ccgaatccgg catgggtatc
     1261 gtcgaagata aagtgatcaa aaaccacttt gcttctgaat atatctacaa cgcctataaa
     1321 gatgaaaaaa cctgtggtgt tctgtctgaa gacgacactt ttggtaccat cactatcgct
     1381 gaaccaatcg gtattatttg cggtatcgtt ccgaccacta acccgacttc aactgctatc
     1441 ttcaaatcgc tgatcagcct gaagacccgt aacgccatta tcttctcccc gcacccgcgt
     1501 gcaaaagatg caaccaacaa agcggctgat atcgttctgc aggctgctat cgctgccggt
     1561 gctccgaaag atctgatcgg ctggatcgat caaccttctg ttgaactgtc taacgcactg
     1621 atgcaccacc cagacatcaa cctgatcctc gcgactggtg gtccgggcat ggttaaagcc
     1681 gcatacagct ccggtaaacc agctatcggt gtaggcgcgg gtaacactcc agttgttatt
     1741 gatgaaactg ctgatatcaa acgtgcagtt gcatctgtac tgatgtccaa aaccttcgac
     1801 aacggcgtaa tctgtgcttc tgaacagtct gttgttgttg ttgactctgt ttatgacgca
     1861 gtacgtgaac gtttcgcaac ccacggcggc tatctgttgc agggtaaaga gctgaaagct
     1921 gttcaggatg ttatcctgaa aaatggtgcg ctgaacgcgg ctatcgttgg tcagccagcc
     1981 tataaaattg ctgaactggc aggcttctct gtaccagaaa acaccaagat tctgatcggt
     2041 gaagtgaccg ttgttgatga aagcgaaccg ttcgcacatg aaaaactgtc cccgactctg
     2101 gcaatgtacc gcgctaaaga tttcgaagac gcggtagaaa aagcagagaa actggttgct
     2161 atgggcggta tcggtcatac ctcttgcctg tacactgacc aggataacca accggctcgc
     2221 gtttcttact tcggtcagaa aatgaaaaca gctcgtatcc tgattaacac cccggcttct
     2281 cagggtggta tcggtgacct gtataacttc aaactcgcac cttccctgac tctgggttgt
     2341 ggttcctggg gtggtaactc catctctgaa aacgttggtc cgaaacacct gatcaacaag
     2401 aaaaccgttg ctaagcgagc tgaaaacatg ttgtggcaca aacttccgaa atctatctac
     2461 ttccgccgtg gctccctgcc aatcgcgctg gatgaagtga ttactgatgg ccacaaacgt
     2521 gcgctcatcg tgactgaccg cttcctgttc aacaatggtt atgctgatca gatcacttcc
     2581 gtactgaaag cagcaggcgt tgaaactgaa gtcttcttcg aagtagaagc ggacccgacc
     2641 ctgagcatcg ttcgtaaagg tgcagaactg gcaaactcct tcaaaccaga cgtgattatc
     2701 gcgctgggtg gtggttcccc gatggacgcc gcgaagatca tgtgggttat gtacgaacat
     2761 ccggaaactc acttcgaaga gctggcgctg cgctttatgg atatccgtaa acgtatctac
     2821 aagttcccga aaatgggcgt gaaagcgaaa atgatcgctg tcaccaccac ttctggtaca
     2881 ggttctgaag tcactccgtt tgcggttgta actgacgacg ctactggtca gaaatacccg
     2941 ctggcggact atgcgctgac cccggatatg gcgattgtcg acgccaacct ggttatggac
     3001 atgccgaagt ccctgtgtgc tttcggtggt ctggacgcag taactcacgc catggaagct
     3061 tatgtttctg tactggcatc tgagttctct gatggtcagg ctctgcaggc actgaaactg
     3121 ctgaaagaat atctgccagc gtcctaccac gaagggtcta aaaatccggt agcgcgtgaa
     3181 cgtgttcaca gtgcagcaac tatcgcgggt atcgcatttg cgaacgcctt cctgggtgta
     3241 tgtcactcaa tggcgcacaa actgggttcc cagttccata ttccgcacgg tctggcaaac
     3301 gccctgctga tttgtaacgt tattcgctac aatgcgaacg acaacccgac caagcagact
     3361 gcattcagcc agtatgaccg tccgcaggct cgccgtcgtt atgctgaaat tgccgaccac
     3421 ctgggtctga gcgcaccggg cgaccgtact gctgctaaga tcgagaaact g
//
LOCUS       DAJTYM010000202         3450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTYM010000202 DAJTYM010000000
VERSION     DAJTYM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..708
                     /locus_tag="OO575_003944"
     CDS             <1..708
                     /locus_tag="OO575_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6666697.1"
                     /translation="ILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDN
                     AGIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNH
                     LAVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDE
                     VGVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNW
                     LSFNWPSYRSRIRGVAA"
     gene            705..2096
                     /locus_tag="OO575_003945"
     CDS             705..2096
                     /locus_tag="OO575_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6666698.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2093..2350
                     /locus_tag="OO575_003946"
     CDS             2093..2350
                     /locus_tag="OO575_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666699.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2445..2645
                     /locus_tag="OO575_003947"
     CDS             2445..2645
                     /locus_tag="OO575_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6666700.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2638..3018
                     /locus_tag="OO575_003948"
     CDS             2638..3018
                     /locus_tag="OO575_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6666701.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            3218..3294
                     /locus_tag="OO575_003949"
     tRNA            3218..3294
                     /locus_tag="OO575_003949"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3252..3254,aa:Ile,seq:cat)
     gene            3296..3372
                     /locus_tag="OO575_003950"
     tRNA            3296..3372
                     /locus_tag="OO575_003950"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3330..3332,aa:Arg,seq:tct)
     gene            3375..3450
                     /locus_tag="OO575_003951"
     tRNA            3375..3450
                     /locus_tag="OO575_003951"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3408..3410,aa:Thr,seq:tgt)
BASE COUNT          893 a          764 c          997 g          796 t
ORIGIN      
        1 atactggtgt ggagcgatcg taatggcgag gacagagaag aaatttcatg ctgccctgac
       61 tgcttaatcg ctgaggccaa cgatttgacc atggagctgt cgtccatcaa ggcggaagag
      121 ctgactgata acgccggaat tgccctgcgt tttcgggact gcgagtttga taattatctg
      181 gaggttaatc ctggcgcagc cagaaatctt gcggcctgtc gccgctatgc ggagaactgg
      241 ccagatatgc tggagaacgg taccagtctt gttatgaccg gcagttgtgg taccggaaaa
      301 aatcatctgg ctgtggctat ggcaaaacac atcatccgta actatctggc cagtgtggag
      361 atcaccgacg tgatgcgcct tacccgtgct gtgaaaaatt gctggcggaa tgacagcgaa
      421 aaaacagcgg atgaagttat tgagcgttat gcgtcaatgg atttgctgat catcgacgaa
      481 gttggcgttc agtttggcag cgcggctgaa atggctattt tgcaggaaat tatcaacgcc
      541 aggtacgaaa gtatcttgcc caccattctg atcagtaacc tctcaccgga agagttgtgg
      601 gcgttcatca gtccccgaat tgccgacagg atcacagacg ggggacgcaa ctggttgtcg
      661 tttaactggc ccagttaccg ttctcgtatc agaggtgtgg ctgcatgaca actccagtat
      721 ggcgtaacga tgaccttgaa ggcgctgtca tcggcgcgtt ctttctgcgc ggagcagatc
      781 ctgaagtgat ggatattctg gccacactgc cagcggacgt tttttctgta cgagcgtatc
      841 aggatatcta tacaggcatc tgcagacagg cccgtgtttc aggagtgatt gaccccgtgc
      901 tgttgtgtaa tgagatgccg gaacttgccc cggtgattac tgataccggg cgtaaaacct
      961 gggtgaagtc ttcactggag cactatgttg cagcgttgcg gcgcaatgcc gcactgcgcg
     1021 atgcagaaaa aacactgaat gaggcgctgc agaaattacg tgatgcgcat acctgtgaag
     1081 cagctgaaga tgccctgaag gatgcgcaga acatgatggt cacactgtcg acaggaaagg
     1141 gcgtcattca gccggtacat attgatgatg tgcttccgga agtggttgag cgtgttgaat
     1201 gccggaatca ggggctggag aaatccagga cgttgatgac cggtattgat gaactggacg
     1261 caaaaacagg cggtatggag cccggagacc tggtattcat tgccgcccgt ccttcgatgg
     1321 ggaaaaccga acttgcgctg gacatcatcg acaaggtgac tgagcagggg catggtgtgc
     1381 ttctgttcac catggagatg gcgaacatcc agattggtga acgtatggtg tctgcggctg
     1441 gagggatgcc agtatcacgc ctgaaatctg tggctcactt tgaagatgaa gactggacgc
     1501 gtttctcaca aggggtgggg cggatgaccg ggcgcaatat ctggatggtg gaccaggcga
     1561 acctggccat tgatgagata tgcgcaacaa cgaaacacca cctgattaaa tatccggaaa
     1621 cggcgctggt ggtggttgat tatctcgggc tgataaaaac ccgaaccacg gggcgtcatg
     1681 accttgccgt gggtgaaatc tcaaaggggc ttaaaggcct ggcaaaatcc ggtggttttc
     1741 cgttgattgc gctgagccag ctctcccgcg gtgtggagtc cagacccaat aaacgtccca
     1801 tgaactcaga cctgaaaaat tccggagaaa tagaggcgga tgcagacatc attctgatgc
     1861 tttacaggga tgaagtgtac aacccggata cgcaggccag gggcatcgca gaaatcaata
     1921 tcacgaaaca acgtaacggt tctctgggaa cgatttaccg gcgtttttat aacggacatt
     1981 ttctgcctgt ggaccaggaa agtgcacggg tgctttccac tcccatgaag cccggcaatc
     2041 cgcgcagata cagtaataaa cgaactgaca gcagtaagat ggagcgtttc ttttgaacaa
     2101 ccagacaatg acttttaccc ctgaacaatt gcgtaaacac gcgcaggaaa tgctgcgtca
     2161 tgctgaacaa ctcgaaaaaa cgggcataac aaaagatgct atccgtaaag atatggtacc
     2221 cgcgcttcgg gaactgatgc aggcgaagca tcgcgcacag aaagcagtag atgagctggt
     2281 ggactgtgtg gcagagctgg aaaccagagt cggaaagttt gaaaaactgg tgcaggaggc
     2341 gctgcgctga tgcgccatga gtttgttttg ccctggcttg tgcgcgatca ttatgtccct
     2401 ggtggaaagc ccgggataaa aatcaccgaa ccggagagta tttgatgagc gtcaaaattc
     2461 aaacgatacc ggaactgctt atccagacac gaggtaatat gaccgaagtt tcacgaatgc
     2521 tgaactgtaa tcgcgccacg gtaagaaaat atgctgaaga taaagagggc aaagggcacg
     2581 ccattgttga tggtgttctg attgttcatc gtggatggga caggggtaaa gacagtgatg
     2641 cgtgatattc agcaggttct tgagcgctgg ggcgcgtggg tggctaataa tcatgaggat
     2701 gtgacctggt cgtccattgc tgccggtttt aagggattaa tcccttcaaa agtaaaatcc
     2761 cgcccgcaat gctgtgacga tgacgcgatg gttatatgtg ggtgtatggc ccgccttaac
     2821 cggaacaaca acgatctaca tgacttgctg gttgactact acgtgtttgg gatgacattc
     2881 atgacgctgg cgcggaagca tggatgctca gatggttata taggaaagaa gctacaaaaa
     2941 gccgaggggg tggtggaagg aatgttgatg atgttgggtg tccggttaga aatggacaaa
     3001 tatattgaac atccctgatg gtaaggggcg actttctaga aaatatttta cgtacgtaaa
     3061 aaacgggata ttctgttaag agtggttgct tcgccacaca gcttaaaccc gcctccgggc
     3121 gggtttttta tgcctgaaaa acggtacagg acgttaaaca cgctggtggt cagatgagtt
     3181 tgcagatgtg atgacatatg gttattattc tgcctccggc cctttagctc agttggtcag
     3241 agcgagcgac tcataatcgc caggtcgctg gttcaagtcc agcaagggcc accaaccacc
     3301 actagctcat ccggatagag catcaacctt ctaagttgac ggtgcgaggt tcgagtcctc
     3361 ggtggtgggc cagcgccgac ttagctcagc aggcagagca actgacttgt aatcagtagg
     3421 tcaccagttc gattccggta gtcggcacca
//
LOCUS       DAJTYM010000203         3443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTYM010000203 DAJTYM010000000
VERSION     DAJTYM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..1955)
                     /gene="metG"
                     /locus_tag="OO575_003952"
     CDS             complement(<1..1955)
                     /gene="metG"
                     /locus_tag="OO575_003952"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6666702.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMV"
     gene            2087..3196
                     /gene="apbC"
                     /locus_tag="OO575_003953"
     CDS             2087..3196
                     /gene="apbC"
                     /locus_tag="OO575_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6666703.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          822 a          871 c          907 g          843 t
ORIGIN      
        1 atcaccatgc cttcagagat accgaagcgc attttacgtg gtgccaggtt agccaccata
       61 atggtgtgac gaccaatcag tgcctgcgga tccgggtaag cagaacgaat accggagaag
      121 acattgcgtt tttcaccgcc gagatccagc gtcaggcgca gcagtttgtc agaaccttca
      181 acaaactctg cgttttcgat cagcgccacg cgcaggtcaa ctttggcgaa gtcgtcaaag
      241 gtgatggttt cctgaatcgg atcatctgcc agtgggccag taactggcgc ggtagtggct
      301 ttcacttctt ctttagaggc ttccaccaga gcttcaacct gtttcatatc gatgcggtta
      361 tacagcgcct tgaacggatt cactttgtgg cccagcagcg gttgctggat accatcccag
      421 gtcagttccg tattgaggaa ggcttctgca cgctcggtca gtttcggcag tacaggcttc
      481 aggtaagtca tcagtacgcg gaacaggttg atgcccattg agcaaatcgc ctgcaggtca
      541 gcatcgcgac cttcctgttt tgcgactacc cacggagcct gctcgtcgac atagcggtta
      601 gccaggtcag ccagtgccat gatttcgcgc actgctttac caaattcacg gctttcccac
      661 gcttcaccaa tcacttcagc ggcatcggta aaggttttgt acagctcagg gtcagccagc
      721 tcgcttgcca gcacaccgtc aaaacgcttg ttgataaagc ccgcattacg ggaggccagg
      781 ttaaccactt tgttcacgat atcggcattc acacgctgaa cgaaatcttc caggttgaga
      841 tcgatatcat caatgcgcga agagagtttc gcagtgtagt agtaacgcag gctgtcagcg
      901 tcaaaatgat tcagccaggt gctggcttta ataaaggtgc cgcgagactt ggacatcttc
      961 gcgccgttca ccgtcacata gccatgaaca aacaggttgg tcggcttgcg gaagttgctg
     1021 ccttccagca tggcaggcca gaacaggctg tggaagtaaa cgatatcttt accgatgaag
     1081 tggtacagct cggcggtgga gtctttcttc cagtattcat cgaagcttac gctgtcgccg
     1141 cgcttgtcgc acagattctt gaaagaaccc atgtagccaa tcggtgcgtc cagccagacg
     1201 tagaaatatt tgcccggtgc gttcggaatt tcaaaaccaa agtatggcgc gtcgcgggag
     1261 atatcccact gttgcaggcc ggattcaaac cactcctgca ttttgtttgc cacctgctcc
     1321 tgcaacgcac cgctgcgggt ccatgcctgc agcatttcgc tgaaagaggg cagatcaaag
     1381 aagaagtgtt cagaatcacg catcactggt gtagcaccag aaaccaccga tttcggctcg
     1441 atcaattcag tcgggctgta ggtcgcgccg cagacttcgc agttatcgcc gtattgatcc
     1501 ggggatttac atttcgggca ggtgcctttc acaaaacggt ccggcaggaa catgcctttt
     1561 tccggatcgt acagctgaga gatggtgcgg tttttaataa aaccgttttc tttcaggcga
     1621 gagtagataa gttctgacaa ctggcggttc tcttcgctgt gcgtcgagtg atagttgtca
     1681 tagctgatgt taaagcctgc gaaatcagtc tgatgctcct gactcatttc gccaatcatc
     1741 tgctccgggg tgataccgag ctgctgagct ttcagcatga tcggtgtacc gtgggcatcg
     1801 tcggcgcaga tgaagttgac ctcgtggccg cgcattcgct ggtaacggac ccagacatca
     1861 gcctggatgt gctccagcat atggccgagg tggattgagc cgttagcgta cggcagtgcg
     1921 cacgtcacca gaattttctt cgcgacttga gtcatagtag gcattacttc ttaatagtga
     1981 aaaggggtta gatagtacca aatgggaaaa tgttaagtaa gtacttctct atattcttca
     2041 ggtaaactat cagctgatgg tttatctata caaaaaagga gtcgggatga acgaacaatc
     2101 ccaggccaaa tcaccagaag ccttgcgcgc aatggtcgcc gggacactgg ccaattttca
     2161 gcacccaacc ctgaagcata acctcaccac gctgaaagcg ttgcaccatg tcgcctggat
     2221 ggacgacacg ttgcatgtgg aactggttat gccgtttgtc tggcatagcg cgttcgaaga
     2281 gttaaaagag caatgtagcg ccgaactgct gcgcatcacc ggggcgaaag ctatcgactg
     2341 gaaactgtcg cataacatag ccactctgaa acgcgtgaag aatcagccgg gaattaacgg
     2401 tgtgaagaac attatcgcgg tgagttccgg taaaggcggc gtgggtaaat cgtccacggc
     2461 ggtaaacctg gcgctggcac tggcggcgga aggggcgaaa gtcggcattc tggacgccga
     2521 tatctacggc ccatcaattc ccaccatgct gggcgcagaa aatcagcgtc caacttcgcc
     2581 ggacggtacc cacatggcac cgattatgtc tcatggcctg gcaaccaatt caattggtta
     2641 tctggtcact gacgacaatg cgatggtgtg gcgtggaccg atggcgggca aggcgttaat
     2701 gcagatgttg caggaaacct tgtggccgga tctcgactat ctggtgcttg atatgccgcc
     2761 aggcaccggt gacattcaat tgacgctggc gcaaaacatt ccagtaactg gtgcagtcgt
     2821 ggtcaccacc ccgcaggaca tcgcgttgat cgatgcgaaa aaaggcattg tgatgttcga
     2881 gaaagtcgag gttccggtgc tcggtattgt cgagaacatg agtgtgcata tttgcagtaa
     2941 ctgtggtcac catgaaccga ttttcggtac cggtggtgca gagaaactgg ctgaaaaata
     3001 ccatacacag ctgctggggc agatgccgtt acacatctcc ttacgtgagg atctcgataa
     3061 aggcacgcca acggttatca gccgtccgga gagcgaattt accgctatct accgccagct
     3121 ggctgaccgc gttgcagctc agctctactg gcagggtgaa gtcattcctg gcgagatttc
     3181 cttccgcgcg gtctaacgcc tattaaccat tctggttatt cttcatacac cgcagagatg
     3241 ttatattgat gcggtgtatt tgtttttgcc gcattaatga acacttattt aatactgcga
     3301 atattctgcg gaccttcgtc cgaaactcta caaagtcgag tgatcgtaca ggcaatgctt
     3361 cattatcatc cttcgtgctg tgtagtggat gtacagctat agtttgttat gcactcattc
     3421 tatccaggca taacctttac tac
//
LOCUS       DAJTYM010000204         3417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTYM010000204 DAJTYM010000000
VERSION     DAJTYM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO575_003954"
     CDS             complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO575_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6666704.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(687..1160)
                     /locus_tag="OO575_003955"
     CDS             complement(687..1160)
                     /locus_tag="OO575_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6666705.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1492..2028
                     /locus_tag="OO575_003956"
     CDS             1492..2028
                     /locus_tag="OO575_003956"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6666706.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2143..2469
                     /locus_tag="OO575_003957"
     CDS             2143..2469
                     /locus_tag="OO575_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6666707.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(2657..2896)
                     /locus_tag="OO575_003958"
     CDS             complement(2657..2896)
                     /locus_tag="OO575_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6666708.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          944 a          758 c          722 g          993 t
ORIGIN      
        1 cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg tcggcttgaa
       61 tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
      121 tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga gtaaagccac
      181 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
      241 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
      301 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
      361 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
      421 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
      481 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
      541 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc atatgacgcc
      601 taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggtacaaaa ggcgtgacag
      661 gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta actataattt
      721 atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat ttcaggaaag
      781 taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc tacttgatcg
      841 atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt tattttcttt
      901 aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt gtcagatgta
      961 aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat tgattcaaaa
     1021 gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag gagctgttca
     1081 cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt cgatatagct
     1141 accattagtg atagtttcac aattcttcct cagaggttaa cattttatta gtaagcatgc
     1201 tcgttttgtc acccgctgat gcttaccgtt aattaatata atcacttgta ttaaatagac
     1261 tttctttaaa aatacaagac actctgttat tacaaatcgt gcatgccgtc tatcctgctt
     1321 gcacgatgca cattatctca cccaaagaac tcataacatt ttaatttatt tagcattaaa
     1381 ttaaaactct accggtaaac aagcatctct aggcgtaaaa aacaggcact tttttaaatc
     1441 ccagctagaa taggctgtat aggcagacag ttgcaagaca aggagcgttt tatgtctaac
     1501 agtccatttt taaattctat acgcacggat atgcgacaaa aaggttatgc gctgaaaact
     1561 gaaaaaactt acctgcactg gattaagcgt tttattctgt ttcacaaaaa acgtcatcct
     1621 cagaccatgg gcagtgaaga ggtcaggctg tttttatcca gcttagcaaa cagcagacat
     1681 gtagccataa acacgcagaa aatcgcttta aatgccctag cttttttgta caacaggttt
     1741 ttacaacagc cgttgggcga tattgattat atccctgcaa gcaagcctag acggctaccc
     1801 tctgttatct ctgcaaatga agtgcaacgc attttgcagg ttatggatac tcgcaaccaa
     1861 gttattttta cgctgctgta tggtgcaggt ttgcgcatta atgaatgctt gcgtttgcgg
     1921 gttaaagatt ttgattttga taatggctgc atcactgtgc atgacggtaa gggtgggaaa
     1981 agcagaaaca gcctactgcc cacgcgccta atcccagcaa taaaataact cattgagcaa
     2041 gcgcggctta ttcagcaaga cgacaactta caaggcgtag ggccatcgct gccttttgct
     2101 ttagatcaca aatacccttc tgcttatcga caagcggcgt ggatgtttgt ctttccctcc
     2161 agcacgctct gcaaccaccc gtataacggc aaattatgcc gccatcatct gcatgactcc
     2221 gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag gcatcgttag caagcgtgtc
     2281 acttgtcata catttcgtca ctcgtttgct acgcatctat tacaagcggg gcgtgatatt
     2341 cgcactgtgc aagaactctt agggcataac gatgttaaga ccacgcaaat ctatacgcat
     2401 gtgttgggtc agcattttgc cggcaccacc agtcctgcgg atggactgat gctacttatc
     2461 aatcagtaaa agtagataaa tatctttatg tagccctgag tagcccctga tactttttat
     2521 ggtgaaaaag ataaacaatc atagagggga taaatatgag ccagttctac cattgttcag
     2581 ataagtttaa gcaagatact gtcaatcaca gtgaccattc atggtcatta cacttcctcc
     2641 agttgccaga caatgcctaa cataaaaacg caaaagccca aaagtcagag cttaggtgtt
     2701 ctaggcgttt tctaagcgct gaagaaatac gtcttaccgt agattgcgtg cgtttctgaa
     2761 gcacattttc cttatacatg gctttatccc aggcagcttc atcaagctct tggagtaaca
     2821 agccagtaat aatgcgacac tctcgggttt gtagcgagcc gctggtgaga tcggagttat
     2881 agcggaaagg ggtcatttgt aattatttgt gggtggagga tagttaactt attattgtag
     2941 ttggaagagg ctgctttcca aacattagca cctcgtaatg cttgttactg tatattaatt
     3001 tatgctctta gcgcatgtta atgggctatt acaatgcata atgtccacat caagataact
     3061 gtggcttaac acctgtacaa gcgaaaaaaa tcaatcacct gtccggaaat acgggatcac
     3121 tgtaaagtgt tggtgaggca gtctaggagg gggatttaat ttgggatact gatgaaagga
     3181 ttatgatcag ttggatatag gatcgtacta agctgagcgg caggttagct agattagtta
     3241 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     3301 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     3361 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagctgc cgaccgg
//
LOCUS       DAJTYM010000205         3357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTYM010000205 DAJTYM010000000
VERSION     DAJTYM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..3219
                     /locus_tag="OO575_003959"
     CDS             <1..3219
                     /locus_tag="OO575_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6666709.1"
                     /translation="LSREGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHT
                     TFSINFDDSVDKTAETDEEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYEL
                     AEHGYIVKTAQSGGASSHILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFS
                     DRLGHSGDKFPLAKAQRDALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAA
                     LEKSEPPVIIATSTNNQAVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRK
                     AEAAKKYQTEDFFNQVESKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAA
                     KSEQLIRLNATWQTLSQIRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWK
                     KYRAGESLIYSLFSWLPAVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLL
                     NSAEREQTTYRQQIDSAHEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQ
                     IRFPAFLLTTHYWEGRWLMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCY
                     MLPGNMQISEHKGQRKFEKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDT
                     EQIPPIWSIAPAIDVGNMLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFA
                     SRYQYDPELARGMYLYEHRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHI
                     DGKGELASSGSRYNLLEAETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIK
                     QVLGKQDISTGTNEKSLTVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVA
                     VSRAKDSFLVFGDMDLFEVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKI
                     YTLHGVEQHDNFLNQTFENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIV
                     TDRSYNTEHNDFEKRKEKQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGS
                     FNWFSATREARYERYDTSMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT          945 a          702 c          867 g          843 t
ORIGIN      
        1 ctaagccgtg agggtttttt atatccgacg ccagcgacct ccattcccag agacctgctt
       61 gaacctttgc caaaaggagc attttcgatt ggtgagattg ggcagtatga caaatacaag
      121 acgacccata ccacgttctc tatcaacttt gatgacagcg ttgataagac tgccgaaacg
      181 gatgaagaac gggaagcacg atatgccgcc ttgcagcagg agtggcgtca atatctgtat
      241 gactcagaga ggctactgaa gagcgttgcc ggcgactgga ttgaaaaacc tgagcaatat
      301 gaactcgctg agcacggtta tattgttaaa acggctcaat ctggcggtgc cagttcccat
      361 atcctttctc tttatgatca cctgcttgtt tgcaataagg atgtgccgct cttcaatcgc
      421 ttcgcctcgc gagaggttca tgctgcagag tctttgctgg ccccaggagc aaaattcagc
      481 gacaggcttg gacactccgg agataagttt ccgctggcaa aggctcagcg cgatgcctta
      541 agccattttc tggatgcaag acatggcgat atccttgctg ttaatggccc tccgggaacc
      601 ggaaaaacca cgctggtgct ttctatcatc gccacgcagt gggccagagc ggctctcgaa
      661 aaatctgagc ctccggttat tatcgcgact tcaacgaata accaggctgt aacgaacatt
      721 attgaggcat tcgggaaaga cttttcgcaa ggttcaggtg cgatggccgg gcgatggttg
      781 ccagagctga aaagcttcgg tgcttatttt ccctcaagca gtcgtaaagc tgaggcagcc
      841 aaaaaatatc aaactgaaga tttcttcaac caggttgagt caaaagagta tgtagaggat
      901 gcactgctgt tttatctgga aaaggctaag gcagcctttc ctggaaaaga gtgttcatcc
      961 cctgaaaagg tcattgaact cttgcatggt cagttggcag caaaatctga gcaactgata
     1021 agactgaacg caacatggca aacgttaagc cagattcggg ctgcgcgtga gcttattgct
     1081 aatgatattg agcaatatct cgataattta aataaattac tttccggaca agaacaaaaa
     1141 gtcactctac tgaagagtgc taaaacggaa tggaaaaaat atcgcgccgg tgaatcactg
     1201 atctattcat tattttcctg gctcccggcg gttcgcaata agcgacagta ccaaatacag
     1261 ctgtttctcg aagataaatt aggcgcgctg attgcaggaa atcagtggtc tgatcctgaa
     1321 actatcgaac gtaatattga tgggctgctc aattccgctg agcgcgagca aacaacatac
     1381 cggcagcaga ttgactccgc ccatgaaatc gttcttaaag aacagcaggc ggttcaggag
     1441 tggcagaggc tggcatttga tttagggtat gagggcgacg aggaactgag cttctcacag
     1501 gccgatgaac tggctgatac gcagattcgc ttccctgcat ttttactgac gactcactac
     1561 tgggaaggtc gttggctgat ggatatggcc agcattgatg atctgcagga tgagaagaag
     1621 aaaaaaggtg ctaaaggggt aaccgcccgt tggcaacgtc gaatgaaact cacgccatgt
     1681 gtggtgatga catgctatat gctgcccggt aatatgcaga taagtgagca caaaggacaa
     1741 cgtaaattcg agaaaagtta tttgtatgat tttgccgatt tactcattgt cgatgaagcc
     1801 gggcaggtgc ttcctgaagt ggctgctgcc tcgtttgcat tagctaagaa ggcattagtg
     1861 attggcgata cggagcagat cccgccaata tggagtattg ctcctgcgat tgatgtcggt
     1921 aacatgctgg cggaaaaaat tctgtctggc agtacgcaag aagagattac cgagaaatat
     1981 acggcaatcg cagaccttgg taaaagtgcc gcatctggca gcgttatgaa aatagcgcag
     2041 tttgcttcgc gctatcaata tgatcccgaa ctggctcgtg gtatgtacct atatgaacac
     2101 cgccggtgct acgacaatat tattggatac tgtaatacgc tctgctatca cggtaagttg
     2161 ttgcctaaaa gagggcgtga agagagcaat ttaatgcccg caatggggta tctccatatt
     2221 gatggtaaag gagagctggc aagtagtgga agtcgatata atttgcttga ggctgaaacg
     2281 atagcggtct ggttggcaga gaaccagcaa aatattgaag cgcattacgg taaatcgctt
     2341 catgaagttg tcggtattgt gacgcctttt agcgctcagg tatccactat caaacaggtg
     2401 ctgggcaaac aagatatcag tacaggcacg aatgaaaagt cgctcacagt gggcaccgtg
     2461 cactctcttc agggagcgga aagagcgatt gtgatattct cgccagtcta ttcaaaacat
     2521 gaagacggcg ggtttattga tagcgataac agcatgctga atgttgcagt ctcccgtgcg
     2581 aaggacagtt ttctggtctt cggcgatatg gacctgtttg aggtccagcc agcctcatcg
     2641 ccacggggat tactggcaaa atacctcttt gagtcagaga agaatgcgct ctcttttgat
     2701 tataaagagc gtaaggattt aaaaaccgcc gggaccaaaa tctacacact tcatggtgtg
     2761 gagcaacatg ataatttcct gaatcagaca tttgaaaata ccagtaaaca catcacgata
     2821 atttctccat ggctgacctg gcaaaggctg gagcaaaccg gttttcttga ttccatgatt
     2881 gcggcgtgtt cacgtggaat taacgtcacg atagtcactg acagaagcta caacactgaa
     2941 cataatgatt ttgagaagcg aaaagagaag cagcagaact ttaaagcggc gctggagaaa
     3001 ctgaatgcgc tgggtattgc tacaaagctg gtaaaccgtg ttcatagcaa aattgttatt
     3061 ggtgatgatg gtttgctgtg tgtgggatcg ttcaactggt ttagtgcgac acgggaagcg
     3121 cgatatgaac gatacgatac atcaatggtt tattgcggtg ataacctgaa gggtgagatt
     3181 gaggctattt ataatagtct tgagaggcgt caggtttaga gcaggaatgg tgagtagcca
     3241 gcttaccgat cgttttcgag cgtaagatgg ctgaatggaa tggctattat tgcacagtcc
     3301 ttaattataa cattcatacc gacatgatta tcttctgtcc ggaagaatca gaggctc
//
LOCUS       DAJTYM010000206         3181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTYM010000206 DAJTYM010000000
VERSION     DAJTYM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            419..1528
                     /gene="fepE"
                     /locus_tag="OO575_003960"
     CDS             419..1528
                     /gene="fepE"
                     /locus_tag="OO575_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6666710.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2553..2747)
                     /locus_tag="OO575_003961"
                     /pseudo
     CDS             complement(2553..2747)
                     /locus_tag="OO575_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          802 a          659 c          775 g          945 t
ORIGIN      
        1 aaaaaaaaac attatcttat attaggggtg ctgctagcgg cgcggtgtgt ttttttacag
       61 gacgcccctg ggggcgctgc taggggtgtc tgttcagata tgtagcaatg agtcagggat
      121 tcacgaactg aaaatgtccg aaaatgtgtc tcatgggcac tgtatgcgcc caaaccgatc
      181 gtcattcact ttcatgcata gctatgcagt gagctgaaag cgatcctgac gcatttttcc
      241 ggtttacccc ggggaaaaca tctctttttg cggtgtttgc ggcagaatcg cgttcagcgc
      301 gttttagcgg tgcgcgcaat aaaatgttat gttaaatgtc tgctgaatgg tttttaggtt
      361 ttttcctaac atatgataga ataaaaaaac catatgttaa tatattgtgt ttttttcaat
      421 gaaaatctca aaggtctctg acgaacattt ttctaattac aacatgttgc cgaatggtaa
      481 tgaagaaatt gacctttttg gcttgattag cgttttatgg gcagcaaaaa taaaaattgt
      541 tggggtgatt ctattttttg cttgtattgg ctttcttgtt tcttttgttt tgccacaaaa
      601 atggactagt tctgctgtgg ttactcctgc tgagtctgtt caatggagtg aattaaataa
      661 ggagctttcc aagcttcatg ttctgggagt ggattttgat atcaatcgtg atagcgcatt
      721 tgctctgttt attaagaaat ttcagtccgt taattcgttg aatgagtata tgagatcatc
      781 tccttatgtt atggagatta ttaaaaagaa taacattagc gctctcgaac tgcatcgtgc
      841 aattgttgag ttgagtaaaa atatgaagtc gattgatgat aatgccagta agaaaaacga
      901 caagtcatca ttgtatgtat catggactct gagttttact gctccaacaa gtaaagaagc
      961 tcacgatgtg ttgtctgggt atattaatta tgtttcttcc cttgttgtaa gggatttgat
     1021 ggaagatata agaaataaac tagaagttaa aactaatgtt gaaaaagaaa ttcttgcact
     1081 ggatgagata aaaattagaa accagctgaa tgcagatatt cgacgcctca attattcact
     1141 ggaggttgct aatgcggctg gaataaaaaa acctgtatac agcaatggtc agattatgaa
     1201 ggatgaccca gattttcctg tggctctcgg ttctgatggt atagcaacta aattgaacat
     1261 caaaaaatca atcaaggatg tttcggaatt gagtggggag ttgcgaaatc gtcaatatgt
     1321 tgtgaatcaa ttggttgtgg cgaaagtggg ggatgttgat tttatgccat ttcagtatca
     1381 attgagccca acgttaccgg tcagaaagga tgggccaagt aatgccataa ttgtaattct
     1441 ttcttctgtc attggaggga tgcttgcatg tggctatata cttttacgtc atgccatgga
     1501 ggccagaaag atgagtgtga ttgagtaaaa gttgtgctga tgcatcattt tataagcaac
     1561 ttttataaaa agttcaaagg atcttcttga gatcattccc ttacagccgt agtcttctac
     1621 tctagaaaac gaaaaaacca cctggggagg tggtttttcg aaggttcagt aagttgggga
     1681 acttctgaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatctgt ccttctagtg
     1741 tagcctcagt aaggctgcca cttcaagaac tctcaataca tctctcgcac atcctgttta
     1801 ccagtggccg ctgccaatgg cgttaagtcg tgtcttaccg ggttggactc aagacgatag
     1861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1921 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacgaac gcggccataa
     1981 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc
     2041 agggggaaac gcctggtatc tttaagtcct gtcgggtttc gccacctctg acttgagcgt
     2101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccac cggcgcggcc
     2161 ttttttcggg gcctcttttt tgctcacatg ttttttctgg tgttatcccc tgattctgtg
     2221 gataaccgta tatccgtctt tgcgtgagca gacaccgctc gccgcagccg aacgaccgag
     2281 cgcagcgagt cagtgagcga ggaagcggaa gagcgccgga agctgcattt tctccttacg
     2341 tatctgtgca gcatttcaca cctcatatgt cgctgttctc tcaccatctg ctctgccgcc
     2401 tcataattaa gccagtatac actccgttat cgctacgtga ctgggtcatg gctgcgcccc
     2461 gacacccgct aaaaccccct gacgcgccct gacgggcttg tctgctgacg aaaacgccca
     2521 cgggaaagcc gtgaccgcct ccgtcagctt cgttatgttc ctgtttcact ttcggcctca
     2581 aagcgaatct ggatgctgtt ctggagttct tctgcgagtt cgtgcagtcg tttgcacatg
     2641 gccgcctgtt cgtcggcatc cagagcatcc agtttttcaa gcagtgtcag gctctggctt
     2701 ttgatacatc gcgccatatt cagcgcagtt tgttgttatt tgttcacctt ccgttgttct
     2761 ccgtcagttc cgggtgtcgg ggggggggac cctgagcagg tggtaattgt aagacgttgc
     2821 gggcgacggt attacaattg cacatcctgt cctgtttctt taggacgtat catggcacag
     2881 catatcaact gataaataat ccctgtgcag tgcagccgct gttgcataaa atttcgctct
     2941 gcgtcgagtg tttcttaatc aataattccg ctgatactcc ggaaaaacgc gagtctgttc
     3001 tcatgcctga aatgcccaca ccccacgcaa aaacaagttt ttgctgattt ttctttataa
     3061 atagagagtt atgacaaatt agttcttctt gctctctttg tgatatttaa aaaagcggtg
     3121 tcggcgcggt gttgtagctg cgccaacacc gctttttagg ggtggtactg actattttca
     3181 t
//
LOCUS       DAJTYM010000207         3119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTYM010000207 DAJTYM010000000
VERSION     DAJTYM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..1941)
                     /gene="flhA"
                     /locus_tag="OO575_003962"
                     /pseudo
     CDS             complement(1..1941)
                     /gene="flhA"
                     /locus_tag="OO575_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(1934..3081)
                     /gene="flhB"
                     /locus_tag="OO575_003963"
                     /pseudo
     CDS             complement(1934..3081)
                     /gene="flhB"
                     /locus_tag="OO575_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          747 a          936 c          772 g          664 t
ORIGIN      
        1 atactggcgg cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta
       61 aacgatccgc cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac
      121 gttccagcgg tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct
      181 gggtaatcgc ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt
      241 ggatgggcgc atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt
      301 tttcatcgag gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa
      361 cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct
      421 cctgacgacc aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta
      481 ccaccgtgct ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac
      541 tttcaatcca gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg
      601 tcccggcagc ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc
      661 caatctccac gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc
      721 gaatgtgcac cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc
      781 gtatacggcc caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac
      841 ccacttccat tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg
      901 tattattctc tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc
      961 gtatccacca ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca
     1021 ttccaggcac caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt
     1081 tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa
     1141 cgatgccccc cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc
     1201 caatggtcaa tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac
     1261 gccgaccagc aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc
     1321 gccgcgaaca aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac
     1381 ttcggagcgg cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc
     1441 aatcgccatc tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat
     1501 acgcccggca cctttggtaa tgaccataaa gttgatgatc acgagaatga cagacaccac
     1561 gataccgata gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc
     1621 agccgccgcc gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag
     1681 tgccagacgc aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt
     1741 gcgctgggta aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa
     1801 caacaggtcg agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat
     1861 caaaatcggt ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag
     1921 catcgcggcc agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca
     1981 ccggaagatg agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg
     2041 ccagacccag gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc
     2101 aatctccgca tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt
     2161 attttcagcg ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac
     2221 tttcggtgcg ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt
     2281 attgacaatg acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg
     2341 ctgcatctga cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc
     2401 acgaatatcc tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac
     2461 gtcaaatccc accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa
     2521 atccatcgca ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg
     2581 ccagtgatgc cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc
     2641 cttaagcaac tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt
     2701 gagtttggaa aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac
     2761 cggagaaatg agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc
     2821 ttctctgatc agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg
     2881 atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc
     2941 aaaccagata acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg
     3001 cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt
     3061 gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatg
//
LOCUS       DAJTYM010000208         3113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTYM010000208 DAJTYM010000000
VERSION     DAJTYM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO575_003964"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO575_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6666711.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO575_003965"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO575_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO575_003966"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO575_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6666712.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO575_003967"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO575_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6666713.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3113)
                     /locus_tag="OO575_003968"
                     /pseudo
     CDS             complement(2163..3113)
                     /locus_tag="OO575_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          758 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag ggg
//
LOCUS       DAJTYM010000209         3101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTYM010000209 DAJTYM010000000
VERSION     DAJTYM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..398
                     /locus_tag="OO575_003969"
                     /pseudo
     CDS             1..398
                     /locus_tag="OO575_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            982..1698
                     /locus_tag="OO575_003970"
     CDS             982..1698
                     /locus_tag="OO575_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666714.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2250..2654
                     /locus_tag="OO575_003971"
     CDS             2250..2654
                     /locus_tag="OO575_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666715.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2717..3101
                     /locus_tag="OO575_003972"
                     /pseudo
     CDS             2717..3101
                     /locus_tag="OO575_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          875 a          628 c          701 g          897 t
ORIGIN      
        1 aacaggcaca ttcagagtta ctttaaagag cttactgaac gtctgaggtg ttagagccat
       61 gactgccgca gcattgctgg cggaaatgcc tgaatcgagc agcctctcga gacgtgaaat
      121 cagcgcactt gtcggtgttg ctcaggtaaa ccgggactcg ggaactctgc gcggtcgacg
      181 aaccattttc ggtgattgtg ctggggaaga acagctctgt acatggcggc gcttgaggcc
      241 ccccccggtt taatctggtg ataaaggcgt tttacatgcg cctacttgca gccggaaacg
      301 ccaaaaaagt ggcactggtt gcctgtatgc gtaagttgct gaccattatg aacgcaatgc
      361 tcagaaaaaa cgaagaatgg aatgaatcgt atttatgaaa actggattgt gggccataaa
      421 tattccaaaa agatagcccg ctccgtttat taccggaacg ggcgagagca tgacattgtc
      481 tgttattata atgagactgc tgattctact tatttttaat ttcaagaaaa gcgttttcta
      541 caaagaatta tgagatttaa tgttttgttt agacaatatt atcattctgt tcaataatga
      601 aagtcttcat caacaacaag taaaaacaac gtccctctca tcgaaccgga cttgcagatt
      661 tcccgcatcc ggctctcttg caacacatca gaccttcacg ttcgggcggt tatggggaag
      721 gtccagcaag ctgtatgagc cctaactgtt tgaataccag tagattacca gaaacttcta
      781 tcgattgaag acattaccaa ctagcgttac taaatgttga ccaagttttg ttcacttgtc
      841 tggttttgat agtagcattc tgcagagtat tttgatatga taaatatgca ttaacaatga
      901 tattattgtt aatttcctag catgaaaatt aaaagatcaa cttttatctc gaatattttt
      961 tacattatca gttagtttct tatgaatgac aattcattgc ttagaaattc ttcattattc
     1021 atcgcctata tgggatgtgt agggtgggtc agtgcttatt cttatggctg gggaacctct
     1081 ttttactatg gatttccatg gtgggttgtt ggtgcaggtt tggatgatgt tgccagaagt
     1141 ttactctatg ctatcatagt aatgggtata ctttttaccg gttgggggat tggtatttta
     1201 ttttttcttc ttataaagaa aagaagtaaa atacaagact tgagtttttt tagactattt
     1261 tttgctatta ccctactgtt tttccctgta atattcgaac ttttaattct caaacaatat
     1321 ttcatacttc cattatcact ttcattcatt attagttcat tggttatttc aataataatt
     1381 cgtatatatg gacggatctt ttctgtatca tgtttttcag atatcccttt tgttagagaa
     1441 caccgcatta agttaattat ggctggtttt ttggtttatt tttggttttt ttcctttctt
     1501 gttggctggt ataaacctca attaaaaaaa gaatatcaga tgctttgtta taataatagt
     1561 tggtattatg ttcttgctcg ttatgattca cggctcgttt tatctagttc atttaaagat
     1621 gacagtaaca gattcttaat atttaataca gaacagtcgg gattttatga aatcaatgat
     1681 gtttatgtca gaaagtaata atcctctccc acaaagtgaa ttaaatgaat atataatgcg
     1741 ttcgaagtat atgtattgag ctgtcatact gagaaacaaa ccttagggca gcacaattaa
     1801 agccagaaac aactatgcaa gaaagttact aaataagtgc tcggaacagt aaaacactca
     1861 ggggggactc agtccggatt atgtaatcag ataaattgcc aaacatcaca aatcaccagc
     1921 cggactgagt gatgccgagc attgcaccaa taccccgtga tgaacgatgc ctgatgcaga
     1981 aagctcccta taaaacaaag aacaaaaact acgaccggcg attcaccgtc atactgatgc
     2041 tgcatcgtgg tgacactgtc tgtcactttc atgttgactg aacaactctg ctgcgaagaa
     2101 ggtgtaccgc cggcgggatt tgattgtccg gcgtctagct tccacttccc tgatggaact
     2161 gaaagttgtt atggccggtc agcttgccct gtccgcagga catgaggtgc tgtgacggac
     2221 agcaccagta acggaaagtg acaatgaaaa tgaatactgc gctgatagtt gcactaatgt
     2281 gtatgtggta tgcggtaccc gctgctgcaa aagagacttt actggctatg ccacgtaatt
     2341 caacagaaca ttgctatgcg gagattaatg tgcacggacc atatggggtg tatttccgag
     2401 tggtcccgca tccccccggg ggaaagagct gggtcgaatg taacagtgac tactattact
     2461 ctgataaacc accgggagta cagatactcg gaacccgtgc agggtgtcgg gtatacggaa
     2521 tctgcgggac cacctcgaca ctgcatgtgg ctggaagagg ggttgtgtgt attaaaaata
     2581 tatgcagtcc acgtgggatg ataattcaca ggatacggaa acgtcctgta gtggctgtaa
     2641 gcgatgagat gtgatcttac ccagcaatag tagacacgcg gctaagtgag taaactctca
     2701 gtcagaggtg actcacatga caaaaacagt atcaaccagc aaaaaaacac gtaaacagca
     2761 ttcgcctgaa ttttgcagtg aagccctgaa gcttgctgaa cgcatcggtg ttgctgccgc
     2821 agcccgtgaa ctcagcctgt atgaatctca gctctatgcg tggcgcagca aactgcagca
     2881 acagatgacc tcgtctgagc gtgaaagtga actgcccgcc tgaagcggca actggcggag
     2941 caggcagagg aactggctat cctccaaaag gccgcgacat actttgcgca gcggctggta
     3001 tacgtggtgt cagcgtcgga caggaataag ccctcgtcag cagttccgtc agcactgcga
     3061 cagcgttgtc ctcgcggcgg cctttacccg gtcaaaacag c
//
LOCUS       DAJTYM010000210         2924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTYM010000210 DAJTYM010000000
VERSION     DAJTYM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..2739
                     /locus_tag="OO575_003973"
     rRNA            <1..2739
                     /locus_tag="OO575_003973"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2835..>2924
                     /gene="rrf"
                     /locus_tag="OO575_003974"
     rRNA            2835..>2924
                     /gene="rrf"
                     /locus_tag="OO575_003974"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          767 a          647 c          919 g          591 t
ORIGIN      
        1 taggttaatg aggcgaaccg ggggaactga aacatctaag taccccgagg aaaagaaatc
       61 aaccgagatt cccccagtag cggcgagcga acggggagca gcccagagcc tgaatcagtg
      121 tgtgtgttag tggaagcgtc tggaaaggcg tgcgatacag ggtgacagcc ccgtacacaa
      181 aaatgcacat actgtgagct cgatgagtag ggcgggacac gtggtatcct gtctgaatat
      241 ggggggacca tcctccaagg ctaaatactc ctgactgacc gatagtgaac cagtaccgtg
      301 agggaaaggc gaaaagaacc ccggcgaggg gagtgaaaaa gaacctgaaa ccgtgtacgt
      361 acaagcagtg ggagcacgct taggcgtgtg actgcgtacc ttttgtataa tgggtcagcg
      421 acttatattc tgtagcaagg ttaaccgaat aggggagccg aagggaaacc gagtcttaac
      481 tgggcgttaa gttgcagggt atagacccga aacccggtga tctagccatg ggcaggttga
      541 aggttgggta acactaactg gaggaccgaa ccgactaatg ttgaaaaatt agcggatgac
      601 ttgtggctgg gggtgaaagg ccaatcaaac cgggagatag ctggttctcc ccgaaagcta
      661 tttaggtagc gcctcgtgaa ttcatctccg ggggtagagc actgtttcgg caagggggtc
      721 atcccgactt accaacccga tgcaaactgc gaataccgga gaatgttatc acgggagaca
      781 cacggcgggt gctaacgtcc gtcgtgaaga gggaaacaac ccagaccgcc agctaaggtc
      841 ccaaagtcat ggttaagtgg gaaacgatgt gggaaggccc agacagccag gatgttggct
      901 tagaagcagc catcatttaa agaaagcgta atagctcact ggtcgagtcg gcctgcgcgg
      961 aagatgtaac ggggctaaac catgcaccga agctgcggca gcgacgctta tgcgttgttg
     1021 ggtaggggag cgttctgtaa gcctgtgaag gtgtgctgtg aggtatgctg gaggtatcag
     1081 aagtgcgaat gctgacataa gtaacgataa agcgggtgaa aagcccgctc gccggaagac
     1141 caagggttcc tgtccaacgt taatcggggc agggtgagtc gacccctaag gcgaggccga
     1201 aaggcgtagt cgatgggaaa caggttaata ttcctgtact tggtgttact gcgaaggggg
     1261 gacggagaag gctatgttgg ccgggcgacg gttgtcccgg tttaagcgtg taggctggtt
     1321 ttccaggcaa atccggaaaa tcaaggccga ggcgtgatga cgaggcacta cggtgctgaa
     1381 gcaacaaatg ccctgcttcc aggaaaagcc tctaagcatc aggtaacatc aaatcgtacc
     1441 ccaaaccgac acaggtggtc aggtagagaa taccaaggcg cttgagagaa ctcgggtgaa
     1501 ggaactaggc aaaatggtgc cgtaacttcg ggagaaggca cgctgatacg taggtgaagc
     1561 gacttgctcg tggagctgaa atcagtcgaa gataccagct ggctgcaact gtttattaaa
     1621 aacacagcac tgtgcaaaca cgaaagtgga cgtatacggt gtgacgcctg cccggtgccg
     1681 gaaggttaat tgatggggtt agcgcaagcg aagctcttga tcgaagcccc ggtaaacggc
     1741 ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacctgc
     1801 acgaatggcg taatgatggc caggctgtct ccacccgaga ctcagtgaaa ttgaactcgc
     1861 tgtgaagatg cagtgtaccc gcggcaagac ggaaagaccc cgtgaacctt tactatagct
     1921 tgacactgaa cattgagcct tgatgtgtag gataggtggg aggctttgaa gtgtggacgc
     1981 cagtctgcat ggagccgacc ttgaaatacc accctttaat gtttgatgtt ctaacgtgga
     2041 cccgtgatcc gggttgcgga cagtgtctgg tgggtagttt gactggggcg gtctcctcct
     2101 aaagagtaac ggaggagcac gaaggttggc taatcctggt cggacatcag gaggttagtg
     2161 caatggcata agccagcttg actgcgagcg tgacggcgcg agcaggtgcg aaagcaggtc
     2221 atagtgatcc ggtggttctg aatggaaggg ccatcgctca acggataaaa ggtactccgg
     2281 ggataacagg ctgataccgc ccaagagttc atatcgacgg cggtgtttgg cacctcgatg
     2341 tcggctcatc acatcctggg gctgaagtag gtcccaaggg tatggctgtt cgccatttaa
     2401 agtggtacgc gagctgggtt tagaacgtcg tgagacagtt cggtccctat ctgccgtggg
     2461 cgctggagaa ctgagggggg ctgctcctag tacgagagga ccggagtgga cgcatcactg
     2521 gtgttcgggt tgtcatgcca atggcactgc ccggtagcta aatgcggaag agataagtgc
     2581 tgaaagcatc taagcacgaa acttgccccg agatgagttc tccctgaccc tttaagggtc
     2641 ctgaaggaac gttgaagacg acgacgttga taggccgggt gtgtaagcgc agcgatgcgt
     2701 tgagctaacc ggtactaatg aaccgtgagg cttaacctta caacgccgaa gatgttttgg
     2761 cggattgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
     2821 taaaacagaa ttagcctggc ggccgtagcg cggtggtccc acctgacccc atgccgaact
     2881 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccc
//
LOCUS       DAJTYM010000211         2744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTYM010000211 DAJTYM010000000
VERSION     DAJTYM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..819
                     /gene="nanT"
                     /locus_tag="OO575_003975"
     CDS             <1..819
                     /gene="nanT"
                     /locus_tag="OO575_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6666716.1"
                     /translation="ANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQ
                     STGKRWPTGVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAV
                     GCCVGGFLGDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIA
                     GILPKLIGGYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSL
                     TFVVILLIGLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            867..1556
                     /locus_tag="OO575_003976"
     CDS             867..1556
                     /locus_tag="OO575_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6666717.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            1553..2428
                     /gene="nanK"
                     /locus_tag="OO575_003977"
     CDS             1553..2428
                     /gene="nanK"
                     /locus_tag="OO575_003977"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6666718.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            2425..>2744
                     /locus_tag="OO575_003978"
     CDS             2425..>2744
                     /locus_tag="OO575_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6666719.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSA"
BASE COUNT          565 a          716 c          819 g          644 t
ORIGIN      
        1 gccaatatcg taatgacact ggcggcggct actgcgctgt ggttctgctt cgccggtaac
       61 ctgcaaaatg ctgcaatcgt cgctgttctc gggctgttat gcgccgcaat ctttatcagc
      121 tttatggtgc aaagtacggg taaacgctgg ccaacgggcg taatgctgat ggtggtcgtg
      181 ttgtttgctt tcctctactc atggccgatt caggcgctgc tgccaacgta tctgaaaacc
      241 gatctggctt ataacccgca tactgtcgcc aatgtgctgt tctttagtgg ctttggcgcg
      301 gccgtgggat gctgcgtagg tggcttcctc ggtgactggc tgggaacccg caaagcgtac
      361 gtttgtagcc tgctggcctc gcagctgctg attattccgg tatttgcgat tggcggcgca
      421 aacgtctggg tgctcggtct gttactgttc ttccagcaaa tgcttggaca agggatcgcc
      481 gggatcttac cgaaactgat tggcggttat ttcgataccg accagcgtgc agcgggcctg
      541 ggctttacct acaacgttgg cgcattgggt ggcgcactgg ccccaatcat cggcgcgttg
      601 atcgctcaac gtctggatct gggtactgcg ctggcatcgc tctcgttcag cctgacattt
      661 gtggtgatcc tgctgattgg tctggatatg ccttcccgcg ttcagcgttg gctgcgcccg
      721 gaagcattgc gtactcatga cgctatcgac ggcaaaccat ttagcggtgc cgtgccattt
      781 ggcagcgcca aaaacgattt agtcaaaacc aaaagttaat cctgttgccc ggtctatgta
      841 ccgggccttt cgctaaggga agatgtatgt cgttacttgc acaactggat caaaaaatcg
      901 ctgctaacgg tggcctgatt gtctcctgcc agccggttcc tgacagcccg ctcgataaac
      961 ccgaaatcgt cgcagccatg gcattagcgg cagagcaggc gggcgcggtt gccattcgca
     1021 tcgaaggtgt ggcaaatctg caagccacgc gtgcggtggt gagcgtgccg attattggaa
     1081 ttgtgaaacg cgatctggag gattctccgg tacgcatcac ggcctatatt gaagatgtcg
     1141 atgcgctggc gcaggcgggc gcggacatta tcgccattga cggcaccgac cgcccgcgtc
     1201 cggtgcctgt tgaaacgctg ctggcacgta tccatcatca cggtttactg gcgatgaccg
     1261 actgctcaac gccggaagac ggcctggcat gccaaaagct gggagccgaa attattggca
     1321 ctacgctttc tggctatacc acgcctgaaa cgccagaaga gccggatctg gcgctggtga
     1381 aaacgttgag cgaagccgga tgtcgggtga ttgccgaagg gcgttacaac acgcctgctc
     1441 aggcggcaga tgcgatgcgc cacggcgcgt gggcggtgac ggtcggttct gccattacgc
     1501 gtcttgaaca catttgtcag tggtacaaca cagcgatgaa aaaggcggtg ctatgaccat
     1561 actggcgatt gatatcggcg gtactaaact tgccgccgcg ctgattggcg ctgacgggca
     1621 gatccgcgat cgtcgtgaac ttcctacgcc agccagccag acaccagaag ccttgcgtga
     1681 tgccttatcc gcattagtct ctccgttgca agctcatgcg cagcgggttg ccatcgcttc
     1741 aaccggaatt attcgcgacg gcagtttgct ggcgctgaat ccgcataatc tcggtggatt
     1801 gctacacttt ccgttagtca aaacgctgga acaacttacc aatttgccga ccattgccat
     1861 taacgacgcg caggccgcag catgggcaga gtatcaggcg ctggaaggcg atataaccga
     1921 tatggtcttt atcaccgttt ccactggcgt cggcggcggt gtagtgagcg gcggcaaact
     1981 gcttaccggc cctggcggtc tggcggggca catcgggcat acgcttgccg atccacacgg
     2041 cccagtctgc ggctgtggac gcacaggttg cgtggaagca attgcttctg gtcgcggcat
     2101 tgcggcggca gcgcagggcg agctggctgg cgcggatgcg agaactattt tcacacgcgc
     2161 cgggcagggc gatgagcagg cgcagcaact gattcatcgc tccgcacgta cgcttgcaag
     2221 gctgatcgct gatattaaag ccacaactga ttgccagtgc gtggtggttg gtggcagcgt
     2281 tggtctggca gaagggtatc tggcgttggt ggaaatgtat ctggcgcagg agccagcggc
     2341 atttcatgtt gatttactgg cggcgcatta ccgccatgat gcaggtttac ttggggctgc
     2401 gctgttggcc cagggagaaa aattatgatg atgggtgaag tacagtcatt accgtctgct
     2461 gggttacatc ctgcgttaca ggacgcgtta acgctggcct tagctgccag accgcaagaa
     2521 aaagcgccgg gtcgttacga attacagggc gacaatattt ttatgaatgt catgacgttt
     2581 aacactcaat cgcccgtcga gaaaaaagcg gaattgcacg agcaatacat tgatatccag
     2641 ctgttattaa acggtgagga acggattctg tttggcatgg caggcactgc gcgccaatgt
     2701 gaagagttcc accatgaaga tgattaccag ctttgtagcg ccat
//
LOCUS       DAJTYM010000212         2713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTYM010000212 DAJTYM010000000
VERSION     DAJTYM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            543..1457
                     /gene="sitA"
                     /locus_tag="OO575_003979"
     CDS             543..1457
                     /gene="sitA"
                     /locus_tag="OO575_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6666720.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            1457..2284
                     /gene="sitB"
                     /locus_tag="OO575_003980"
     CDS             1457..2284
                     /gene="sitB"
                     /locus_tag="OO575_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6666721.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            2281..>2713
                     /gene="sitC"
                     /locus_tag="OO575_003981"
     CDS             2281..>2713
                     /gene="sitC"
                     /locus_tag="OO575_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6666722.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILS"
BASE COUNT          722 a          633 c          654 g          704 t
ORIGIN      
        1 gccagccaga tggggcatga aactgcgcaa atggtgtatg aaatttacgg tatgtggatt
       61 gatgacatga acgacgaaca gatagccatg ttgaatgcgc ggttatcgta gttgcaaagt
      121 ttgcccccaa tttgccccat ttagtaccag agaactgaaa taatgcaaga aaatcaacaa
      181 attacaaaga aagaacaata caacctgaac aagtaagggc aaaaatcaca actatctgac
      241 tcataagtat tttatttatt tttcaatatg ttaaacatta tcagcgacaa caataagcta
      301 cgataaccca ctcttttttg ccccattttt gccatcaaca attttcctca taaagtcatt
      361 tcaaagccta atagctgctc tacttttttc ctcaatatca tgtcgccagc aggaaacaac
      421 ctaatccctg ttttaggcct caaagctgtt aatgataatt attttcattt tgtataggtt
      481 atgctataaa acatagccgt tgctatgttt gttctaattt tgctcactat aggtactaaa
      541 ttatgcactc aataaaaaaa gtaaccatgc tcttggggtg cctcgccctc acctgctcga
      601 tcgcatttca ggcaagtgca actgaaaaat tcaaggtcat tacaacattt accatcatcg
      661 cggatatggc caaaaacgtg gctggagatg ctgcagaagt ctcatccata accaagcctg
      721 gtgcagaaat tcatgagtat cagcctaccc ctggcgatat taaacgtgcg cagggggcac
      781 aactgattct cgccaatggt atgaatctgg aattgtggtt ccaacgcttt taccagcatc
      841 tcaatggggt tccagaagta attgtctctt cgggtgtgac gccagtaggg atcaccgaag
      901 gaccctatga gggcaaacct aatccccatg catggatgtc gccagataat gctctgattt
      961 acgtcgataa tattcgtgat gcgttgataa aatacgatcc ggcaaatgca caaacctacc
     1021 aacgtaatgc cgatacttat aaagccaaga ttacccaaac ccttgccccc ctgcgtaagc
     1081 agattacgga actccctgag aatcagcgat ggatggtcac cagtgaaggg gctttttctt
     1141 atctcgcacg cgatttgggg ctaaaagagc tttatctgtg gccgattaat gccgatcaac
     1201 aaggaacacc gcagcaggta cgtaaggttg ttgatatagt taagaaaaat catatcccgg
     1261 cagtctttag cgagagtacg atttccgata aaccagcgcg tcaggttgcg cgtgaaaccg
     1321 gcgcacacta cggcggtgtg ctctatgtcg attccctgag cacagaaaac ggcccggtac
     1381 ctacgtatat cgaccttctt aaggttacta ccagtacgct ggtacaggga attaaagccg
     1441 ggaaaaggga gaaataatga tgcaatctgc aggcattgtc gttaatgatg ttactgtcac
     1501 ctggcgtaac gggcacacag cactgcgcga tgcatccttc acagtaccaa gtggatctat
     1561 tgccgctctg gttggggtaa acggttccgg aaaatcgacg ttgtttaaag cgattatggg
     1621 atttgtgcgt ctgaccagcg gaaagatatc tgttctgggt attcccacgc gacaggcgct
     1681 acagaaaaac ctggttgcct acgtccctca gtcagaagag gttgactggt catttcctgt
     1741 actggtggaa gatgtggtga tgatgggccg ctatgggcat atgggtatgt tgcgtatagc
     1801 caaaaagagg gatcgtcaga ttgtcaccga tgcgctggaa cgtgtcgata tggtggattt
     1861 tcgccatcgt caaatcggcg agctttccgg tggacaaaag aaacgcgtct ttctggcgag
     1921 agcaattgca cagcagggtg atgtgatcct gctggatgaa ccttttaccg gcgttgatgt
     1981 gaaaaccgaa gcgaaaatca tcagcctgtt gagggagtta cgcgcggaag gcaaaacgat
     2041 gcttgtctca acgcacaatc tcggctcagt cacgacattt tgcgattata cggtcatggt
     2101 caaaggcacc gtcctggcaa gcgggccgac agacaccact tttacagccg agaacctgga
     2161 gctggctttt agcggcgtac tgcgccatgt cacacttaac ggctcggaag aaagcattat
     2221 tactgacgat gaacgccctt ttgtggcaca tcgaccgtca gcggtgcaga gggaagcaag
     2281 atgaacgtgc tactggaacc cttcagctat gaatatatgc tcaatgcgat gtgggtctcg
     2341 gcaatggtcg gcggtctctg cgctttcctg tcatgctatc tgatgctaaa aggctggtcg
     2401 ctgattggcg atgcgctgtc gcactctatt gtcccgggtg ttgcgggggc atatatgctg
     2461 gggcttccgt tttctctggg agccttcttt tccggcggac tggcagcggg cagcatgctg
     2521 tttcttaacc aacgcacccg cttaaaagaa gatgccatca tcggcctgat cttctcgtcc
     2581 ttttttggcc tggggctgtt tatggtgtca ttgaacccga catccgtgaa cattcagacc
     2641 atcgtcctgg gcaatattct ggcaatcgat ccggcggata tcctgcaact cactatcatt
     2701 ggcattctct cga
//
LOCUS       DAJTYM010000213         2646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTYM010000213 DAJTYM010000000
VERSION     DAJTYM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..85
                     /locus_tag="OO575_003982"
     CDS             <1..85
                     /locus_tag="OO575_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6666723.1"
                     /translation="YYVSTVEASEITPGEADAVVNVTLDYR"
     gene            165..860
                     /locus_tag="OO575_003983"
     CDS             165..860
                     /locus_tag="OO575_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6666724.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            889..>2646
                     /locus_tag="OO575_003984"
     CDS             889..>2646
                     /locus_tag="OO575_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6666725.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLL"
BASE COUNT          776 a          623 c          583 g          664 t
ORIGIN      
        1 ttactacgta tcaaccgttg aagcatcaga aatcacccca ggggaagcag acgctgtcgt
       61 taacgtaacg ctggattacc gttaatacgt tacggcgtta tctgaccttt cagataacgc
      121 ccttttcctt cctctttctc gttgtatcag gttgaaaaat aactatgtca aaacgaacat
      181 tcgcggtgat attaaccttg ttgtgtagct tctgtatcgg ccaggcgctt gcagggggta
      241 tcgttttaca gcgaacgcga gtgatctatg atgccagccg caaagaggct gcgttacctg
      301 tcgcaaacaa aggcgcagaa acgccttatt tactgcaatc atgggtagat aatatagatg
      361 gtaaaagccg tgccccattt attataaccc caccgctatt tcgtcttgag gctagcgatg
      421 actcatcact gcgaattatt aaaacagctg ataacctgcc tgaaaataaa gagtcgctgt
      481 tctacattaa tgttcgtgcc attccagcaa agaaaaaatc agatgatgtt aatgctaacg
      541 agttgacgct ggtatttaaa acgcgggtca aaatgtttta tcgccccgca cacctgaagg
      601 gacgggtaaa cgatgcgtgg aaatcactgg aatttaaacg tagtgaccat tcactcaata
      661 tatataaccc aactgaatat tacgtcgtat ttgccggact ggcagtcgat aaaaccgatc
      721 tcacaagcaa aattgaatat atcgcgcccg gagaacataa acagttacca cttccagcat
      781 ctggcggaaa gaacgtgaaa tgggctgcga tcaacgatta tggcggcagt tccgggacag
      841 aaactcgtcc actgcaataa aaaatataaa aacacaggcc atcagggaat gccacaacga
      901 caccaccagg gacataaacg cacaccgaaa cagttggcgc tcattatcaa acgttgtttg
      961 ccgatggtgc tcactggcag cggcatgctt tgcactaccg ctaacgccga agagtattat
     1021 ttcgacccca ttatgctgga aactacaaaa agtggtatgc aaacaaccga tctgtcacgt
     1081 ttttcaaaaa aatacgcaca actaccagga acttatcagg ttgatatctg gctgaataaa
     1141 aagaaggttt cacagaaaaa aattacattt accgccaatg cagagcaact tctgcagcca
     1201 cagtttacgg tagaacaact acgtgagctg ggtattaagg tggatgaaat cccggcgctg
     1261 gctgaaaaag atgacgatag cgtgatcaac tcgcttgaac aaatcattcc cggtacagct
     1321 gctgaatttg atttcaatca tcagcgactt aatttgagca ttccccaaat tgcactgtac
     1381 cgtgatgcaa gaggttacgt ctccccttct cgttgggacg atggtatacc aacgctgttt
     1441 accaactact cgtttacagg ttctgataac cgttaccgcc agggcaatcg tagccaacga
     1501 cagtacctga atatgcaaaa tggtgctaat tttggcccct ggcgattacg taactattct
     1561 acgtggacac gcaacgatca ggcgtcaagc tggaacacta tcagtagtta tttacaacgt
     1621 gatatcaagg cgttgaagtc tcagttgctt ctgggagaaa gcgccaccag cggcagtatt
     1681 ttttccagct acgcctttac tggcgtgcaa ctcgcttccg acgataatat gttgccaaac
     1741 agccagcgcg gatttgcccc aacggtacgc ggtatcgcaa acagtagtgc aatcgtgact
     1801 atcaggcaaa atggttatgt gatctatcaa agcaacgtgc cagcgggtgc ctttgaaatt
     1861 aacgatctct acccctcttc caacagcggc gatttagaag tcacgattga agaaagtgac
     1921 ggtacacaac gtcgctttat ccagccttat tcttcattac ccatgatgca gcgacctggg
     1981 catctaaaat atagcgcgac cgctggacgc tatcgcgctg atgcaaacag tgatagcaag
     2041 gaacccgaat ttgctgaagc cacggcaata tatggtttga ataatacttt tacgctgtat
     2101 ggcggcctgc tcggttctga agattattat gcgctgggga tcggtatcgg cggcacactt
     2161 ggcgcactgg gcgcgttgtc gatggatatc aacagagctg acacccaatt cgataaccag
     2221 cactcttttc atggctatca atggcgtact cagtacatca aagatatccc ggaaaccaac
     2281 accaatatcg ctgttagcta ctatcgctat accaacgatg gctattttag ttttgatgaa
     2341 gccaataccc gcaattgggg ctataacagt cgccaaaaaa gtgaaattca attcaacatc
     2401 agccagacaa tatttgatgg ggtaagtctg tatgcctccg gttcacagca agactattgg
     2461 ggcaataacg agaaaaacag taatatctct gttggggttt ccggccagca atggggaatt
     2521 ggttacagcc tgaattatca atacagccgc tacactgatg aaaataatga ccgtgcactc
     2581 tctttgaatc tcagtattcc gttagaacgc tggtggattt gcccctatat ttccagacat
     2641 ctgtta
//
LOCUS       DAJTYM010000214         2618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTYM010000214 DAJTYM010000000
VERSION     DAJTYM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..545)
                     /locus_tag="OO575_003985"
     CDS             complement(<1..545)
                     /locus_tag="OO575_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666726.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="OO575_003986"
     CDS             complement(1207..2196)
                     /locus_tag="OO575_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666727.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2618)
                     /locus_tag="OO575_003987"
     CDS             complement(2280..>2618)
                     /locus_tag="OO575_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666728.1"
                     /translation="EAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAK
                     ELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADL
                     ESGGNWMDSL"
BASE COUNT          681 a          612 c          626 g          699 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa ccgtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc ggcgctgtag ctctttttgc cattcgtctt cggtcatgaa catttcgcgg
     2461 gtacgctctt tctgagacgc tagctcttta gcgatacgct cagcttcagc tcgtttgttc
     2521 atctggcggc gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg
     2581 ccagcttcag ccagagcttc cagtaactcc atcgcctc
//
LOCUS       DAJTYM010000215         2571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTYM010000215 DAJTYM010000000
VERSION     DAJTYM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>2571
                     /gene="cusA"
                     /locus_tag="OO575_003988"
     CDS             <1..>2571
                     /gene="cusA"
                     /locus_tag="OO575_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6666729.1"
                     /translation="RSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSDVQVIIKT
                     SYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTDPYWARSR
                     VLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQDWFLKYE
                     LKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAGGSSIELA
                     EAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIAELNGEGE
                     VAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAIDNLSGK
                     LLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMSLGGIAIA
                     VGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPALFISLLII
                     TLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIPPESSN
                     PLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEGDLLYM
                     PSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVETTIQLK
                     PQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIKVSGTV
                     LADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVADVQLFV
                     TSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLADVADVK
                     VSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAFSGQFE
                     "
BASE COUNT          564 a          623 c          798 g          586 t
ORIGIN      
        1 gtcgctcggt ggcgaaccgt tttttggtgc tgatgggcgc gttgtttctg agcatctggg
       61 gcacctggac catcattaat acgccagtgg atgcgttgcc ggatctctcc gatgtgcagg
      121 tgattattaa aaccagctat cccggccagg caccgcaaat cgttgaaaat caggtgactt
      181 atccgctaac caccaccatg ttgtcggtgc ctggcgcgaa gactgtgcgt ggtttctcgc
      241 agtttggcga ctcttatgtg tatgtcattt tcgaagatgg caccgatccg tactgggcgc
      301 gttcgcgggt gctggagtac ctcaaccagg tacagggtaa gctgcctgcg ggagtcagcg
      361 ccgagctggg accagatgcc acgggtgttg gctggatcta tgaatatgca ctggtggatc
      421 gcagcggtaa gcacgatctt gccgatttac gctcattgca ggactggttt ctcaaatatg
      481 agctgaaaac cattcctgac gttgcggaag tggcgtcggt aggcggtgtg gtgaaagagt
      541 atcaggtggt tatcgatcct cagcgcctgg cgcagtatgg catcagtctc gccgaagtaa
      601 aaagcgcgct ggatgcttca aaccaggaag cgggcggttc gtcaatcgaa ctggcggaag
      661 cagaatatat ggtgcgcgcc agcggctatc tacaaacgct cgacgacttt aatcacatcg
      721 ttttaaaagc cagtgaaaat ggcgtgcccg tttatctgcg cgatgttgcg aaggtccaga
      781 ttggcccgaa gatgcgccgg ggcattgccg aactaaacgg cgaaggcgaa gtggcgggcg
      841 gggtggtgat cctgcgatcc ggcaaaaacg cccgagaagt gatcgccgcc gtgaaggaca
      901 aactggaaac gctgaaaagt agtctgccgg aaggcgtgga gatagttaca acatacgacc
      961 gcagccagct cattgaccgg gccatcgaca acctcagcgg caagttgctg gaagagttta
     1021 ttgtggtggc ggtagtctgc gcgccgtttc tctggcatgt gcgctcggcg ctggtggcga
     1081 ttatttcgtt gccgctgggg ttgtgtattg cttttattgt catgcccttc caggggctga
     1141 atgccaatat tatgtcgctg ggtggcattg cgattgccgt cggggcgatg gtcgatgccg
     1201 ctatcgtcat gatcgagaat gcgcataaac agctggaaga gtggcagcac cagcatcctg
     1261 acgccacgct ggataataaa acgcgctggc aggtgatcac cgatgcgtct gttgaagtgg
     1321 ggccggcgct gtttatcagt ctgctgatta tcacgttgtc gtttatcccg atcttcaccc
     1381 tggaagggca ggaagggcgt ctgtttggcc cgttggcgtt caccaaaacg tatgcgatgg
     1441 cgggtgcggc gctgctggcg atcgtagtga tccctatcct gatgggttac tggatccgtg
     1501 gcaaaattcc gccggaaagc agtaatccgc tcaatcgctt tttgattcgt gtttatcatc
     1561 cgctgttgct gaaagtactg cactggccga aaaccacgct gctggtggcg gcgctttcgg
     1621 tgctgacggt tctctggccg ctcaataaag ttggcgggga atttttaccg cagatcaatg
     1681 aaggcgactt gttgtatatg ccatcgacgc tgccggggat ttccgcagca gaggcggcga
     1741 gtatgctgca aaaaaccgac aagctaatta tgagcgtacc tgaagtggcg cgggtatttg
     1801 gcaaaaccgg gaaagcggaa accgccaccg attctgctcc gctggagatg gtagaaacga
     1861 ccatccagct taagccacag gatcagtggc ggccaggcat gacaatggac aaaatcattg
     1921 aggaactgga taacaccgtg cgtctgccgg ggctggcgaa tctgtgggtg ccgccaattc
     1981 gtaaccgtat cgatatgctc tcaaccggca ttaaaagccc catcggcatt aaagtttccg
     2041 gcactgtgct ggcggatatc gacgcgatgg ctgagcaaat tgaagaagtg gcgcgaacgg
     2101 tgccaggcgt agcttctgcg cttgctgagc ggctggaagg tgggcgctat atcaacgttg
     2161 agattaaccg tgaaaaagcc gcacgttacg gtatgacggt ggcggatgtg cagttgtttg
     2221 tgacttctgc ggtgggcggg gcgatggttg gcgaaacggt ggaagggatt gcccgttatc
     2281 caattaatct gcgttatccg caaagctggc gcgatagtcc gcaggcactg cgccagttgc
     2341 cgatcctgac gccgatgaag cagcaaatca ccctggcaga cgtggccgac gttaaagtct
     2401 ctaccggacc gtcgatgctg aaaaccgaga atgcgcgccc gacgagctgg atttatatcg
     2461 atgcccgcga tcgtgacatg gtgtcggtgg ttcacgattt gcaaaaagcg atagctgaaa
     2521 aagtgcagtt aaaaccgggc accagcgtgg cattctccgg gcagttcgag c
//
LOCUS       DAJTYM010000216         2535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTYM010000216 DAJTYM010000000
VERSION     DAJTYM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..350
                     /locus_tag="OO575_003989"
     CDS             <1..350
                     /locus_tag="OO575_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6666730.1"
                     /translation="EIWFYEHPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASR
                     VENEQAWKVSIDDVIARNFNLDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLR
                     DILGAALSGKEVN"
     gene            350..2011
                     /locus_tag="OO575_003990"
     CDS             350..2011
                     /locus_tag="OO575_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6666731.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            2232..>2535
                     /gene="symE"
                     /locus_tag="OO575_003991"
     CDS             2232..>2535
                     /gene="symE"
                     /locus_tag="OO575_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6666732.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQE"
BASE COUNT          781 a          526 c          574 g          654 t
ORIGIN      
        1 aagagatttg gttctatgaa catccgtatc cggcgggcgt gaaaaactac agcaaaacca
       61 agccgatgaa gtttgaagag tttcaggcgg agatcgactg gtggggtaac gaggccgatg
      121 gttttgccag ccgcgtcgag aatgagcaag cgtggaaagt cagcattgat gacgtgattg
      181 cgcgcaactt caatctggat attaaaaacc cacatcaggc ggaaaccgtc agccatgatc
      241 cggacgaact gttagcgcag tatgcaaaac agcaggcgga gatccagacg ctgcgtaatc
      301 aactgcgcga tattcttggc gctgcgctgt ctggcaagga ggttaactaa tgagtgtgga
      361 aaagctgatc gttgatcata tggaaacctg gacctcggcg ttgcaaaccc gttccaccgc
      421 cgggcgcggc agctccggta agattgattt gtatggtatt aagaaattgc gtgagctgat
      481 tctggaactg gcggtgcgcg gtaaactggt gccgcaggat ccgaatgatg aaccggcgtc
      541 ggacctgctg aagcgtattg cggcggaaaa agcagagctg gtgaagcaag gcaaaattaa
      601 aaagcaaaaa ccactgccag aaattagtga ggaagagaag ccgtttgaat tgccggaggg
      661 atgggagtgg gtgcacctcc ctgatattta ttgctcaatt tctgagtcat ctagaaaaat
      721 aaaatcctcc gaaatactac ctgagggaaa atatcctgtt atcgagcaaa gccaagaatt
      781 tatttctggt tattgcaaca atgaatgtct gttaataaag ttaaataatc cagttatagt
      841 ttttggtgat cacacaagga atattaaatt tattgatttt gattttgttg tcggcgctga
      901 cggtgtgaag attttatcac ctatattaat ttgtgagcgt ttcttttttt ggcagttacg
      961 atcatttaaa ttagatgtaa gaggatacgc gagacatttt aaagtattaa atagctgctt
     1021 atttgcatta ccccccattg cagaacaaga gcgaatagtt gaaaaagtta gctctttgat
     1081 gtccctctgc gaccaactag aacagcaatc cctgaccagt ctggacgcac atcagcaact
     1141 ggttgaaacc ctgttgggaa cgcttacaga cagccaaaac accgcggaac tggctgaaaa
     1201 ctgggcgcgt attagcgagc atttcgacac cctatttacc accgaagcca gcgtggatgc
     1261 gttaaaacag accattctgc aactggccgt aatgggcaaa cttgtgccgc aggatccgaa
     1321 tgacgaaccc gcctctgaac tgctcaaacg tattgcgcag gaaaaagctc aattggtgaa
     1381 agaagggaaa ataaaaaaac aaaaaccact gccgccaatt agcgatgagg aaaaaccgtt
     1441 tgaacttccg gaagggtggg aatggtgtcg tattggtaat atagttaata ttaaatctga
     1501 attagtatca ccaaaagatt atttaaattt atatcaagta gcacctgata ttattgaaaa
     1561 gggcactggg agagtgatat caaagagaac agtgaaagaa tcaggagtca aagggccaaa
     1621 tagtcgcttt tataaaggac aaattgttta ttctaaaatc cgcccgtcat tatccaaagt
     1681 ttttttagca gagtataatg gtctttgcag tgctgatatg tatccattag actgttacat
     1741 taatccaaat tacttattga aatatatcct ttccattcca tttcttatgc aagtcaaaaa
     1801 agcagagaac agaataaaaa tgccaaaact taattctgac agtttttaca atattattgt
     1861 tgcgattccc ccctataatg aacaacaagc aatatttgat aagataaatt ctattgaagc
     1921 agtatgcaat ggtcttattt cttatattgg gatttatcat aaaacccaac tgcatcttgc
     1981 agacgcactc actgacgcag cgataaacta attccctcgc cctcacaaaa ccaataaagc
     2041 ggctaaatag ccgctttatt cacatcacca aaatttatat ttcacgctaa ttttcatctt
     2101 attttcatta atttttagct ggactttccc tgtatttact gatgatgtat ccaggtattt
     2161 agcacggtgc ggatgtgcgc caacacactc gcaccgctaa tcacaatcac tatttataga
     2221 gaatagtaat catgactgac acgcattcta ttgcacaacc gttagaagca gaagtctccc
     2281 cggcaaataa tcgtcaatta accgtgagtt atgcgagtcg ttacccggat tacagccgta
     2341 ttcccgccat caccctgaaa ggtcagtggc tggaagccgc cggttttgcc accggcacgg
     2401 cggtagatgt caaagtgatg gaaggctgta ttgtcctcac cgcccaaccc gccgccgccg
     2461 aagagagcga actgatgcag tcactacgca aggtgtgtaa gctgtcggcg cgtaaacaaa
     2521 ggcaggtaca ggaat
//
LOCUS       DAJTYM010000217         2491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTYM010000217 DAJTYM010000000
VERSION     DAJTYM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            32..670
                     /locus_tag="OO575_003992"
     CDS             32..670
                     /locus_tag="OO575_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6666733.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            673..1836
                     /locus_tag="OO575_003993"
     CDS             673..1836
                     /locus_tag="OO575_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6666734.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1920..>2491
                     /locus_tag="OO575_003994"
     CDS             1920..>2491
                     /locus_tag="OO575_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6666735.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGS"
BASE COUNT          573 a          597 c          703 g          618 t
ORIGIN      
        1 cgcagcttgt atgacatggt aatggacgat catggccaga aaacgcaaat caagaaataa
       61 cagtaaaatt ggtcacggag cgatcagtcg cattggtaga ccgaataatc cttttgaacc
      121 gcgtcgcaat cgctacgcac aaaaatactt aacgttggcg ctaatgggcg gtgccgcttt
      181 tttcgtattg aaaggttgta gcgatagtag cgatgtcgat aacgacggcg acggaacatt
      241 ttacgcgaca gtgcaggatt gtattgatga cggtaataat gccgacattt gcgcgcgtgg
      301 ctggaacaac gccaaagcgg cattttatgc cgatgttcca aagaatatga ctcagcagaa
      361 ctgtcagtct aagtacgaaa attgctacta cgacaatgtt gaacagagtt ggatcccggt
      421 cgtttctgga tttttgttaa gccgggttat tcgtaaagat cgcgatgagc cgttcgtcta
      481 taacagcggc ggatcctcat ttgcttcccg ccccgtctgg cgcaacactt ccggtgatta
      541 ctcctggcgc ttcggttctg gcaaaaaaga gtcttactct tcaggcggct ttaccaccag
      601 aaaagcgtcg accgtttcgc gcggcggcta tggtcgttct tccagcgccc gtgggcattg
      661 gggaggctaa tcatgctgag acacaacgtt cctgtgcggc gggatctgga ccagatcgcc
      721 gctaacaacg gtttcgactt tcatatcatc gacaatgaaa tctattggga cgagagtcgg
      781 gcttaccgtt ttactctgcg ccagattgaa gagcagatcg aaaaaccgac tgcggaactg
      841 catcagatgt gccttgaggt ggtggatcgc gcggttaaag acgaagagat cctgacgcaa
      901 ctggcgatcc cgccgttgta ctgggatgtg atcgccgaaa gctggcgcgc acgcgatcct
      961 tcgctgtatg gccggatgga ttttgcctgg tgtggcaatg cgccggtgaa gctgctggag
     1021 tacaacgccg atacgccgac ctcgttgtat gagtcggctt atttccagtg gttgtggctg
     1081 gaggatgccc ggcgcagcgg cattattccg cgtgatgccg atcagtacaa cgccattcag
     1141 gaacgcctga tttcgcgctt tagtgagctt tacagtcggg aactgtttta tttttgctgc
     1201 tgtcaggaca ccgatgaaga caggagtacc gtgctgtact tgcaggactg cgcccagcag
     1261 gcagggcagg agtcgcggtt tatctacatt gaagatctcg gtttgggcgt cggcggcgta
     1321 ctgaccgatc ttgatgataa tgtcatccag cgtgcattta agctgtatcc gctggagtgg
     1381 atgatgcgtg acgataacgg tccgctgctg cgcaagcgtc gcgagcaatg ggtggagccg
     1441 ttatggaaaa gtatcttgag taataaaggg ctaatgccgc tgctttggcg cttcttccct
     1501 ggtcatccta accttcttgc atcctggttt gagggtgaaa aatcgcagat tgccgctggc
     1561 gaaagctatg tgcgtaaacc gatctactcg cgcgaaggcg gaaacgtcac cattttcgac
     1621 ggtcagaata acgtcgttga ccacgctgat ggtgattacg ccgatgaacc gatgatctac
     1681 caggcgtttc aacctctgcc gcgatttggc gatagctaca cactcatcgg tagctggatt
     1741 gtcgatgatg aagcgtgcgg aatggggatc cgtgaagata acacactgat caccaaagac
     1801 acctcacgtt tcgttccgca ttacattgct ggataagaat gttttagcaa tctctttctg
     1861 tcatgaatcc atggcagtga ccatactaat ggtgactgcc attgatggag ggagacacag
     1921 tgcactggca aactcacacc atttttaatc aacctatacc attaaataac agcaacttat
     1981 acctgtctga tggcgcgctt tgcgaagcgg taacgcgtga aggtgctggc tgggatagcg
     2041 attttctagc cagtattggt cagcagttag gaacggctga atcccttgaa ctggggcggc
     2101 tggcgaatgt aaacccgcct gaactgttgc gctacgatgc ccaggggcgg cgtctggacg
     2161 atgttcgttt ccatcccgca tggcacctgc tgatgcaggc gttatgtacc aatcgggtgc
     2221 acaatcttgc ctgggaagaa gacgctcgct ccggcgcatt tgtggcgcgc gcggcgcgtt
     2281 ttatgttaca tgcgcaggtt gaggctgggt cgttatgtcc agtaacgatg acctttgccg
     2341 ccacgccatt gttgttacag atgttaccca cgccgtttca ggactggacc acgccgctgc
     2401 tgagcgatcg ctacgattct cacttattgc caggtgggca aaaacgcggt ttgttgattg
     2461 gcatgggaat gacggaaaag cagggcggtt c
//
LOCUS       DAJTYM010000218         2358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTYM010000218 DAJTYM010000000
VERSION     DAJTYM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..320
                     /locus_tag="OO575_003995"
     CDS             <1..320
                     /locus_tag="OO575_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6666736.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYA
                     SMA"
     gene            477..1226
                     /locus_tag="OO575_003996"
     CDS             477..1226
                     /locus_tag="OO575_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6666737.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1248..1814
                     /gene="yieF"
                     /locus_tag="OO575_003997"
                     /gene_synonym="chrR"
     CDS             1248..1814
                     /gene="yieF"
                     /locus_tag="OO575_003997"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6666738.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1868..>2358)
                     /gene="adeP"
                     /locus_tag="OO575_003998"
     CDS             complement(1868..>2358)
                     /gene="adeP"
                     /locus_tag="OO575_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6666739.1"
                     /translation="EKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGG
                     RTGLTAVVVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESV
                     PAFITAVMMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFID
                     AH"
BASE COUNT          628 a          610 c          591 g          529 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactcca tcaactgccg ttggttgctc
      181 ttcctctccc gtttgacgaa agccagcaaa aaaagctgga agttcctttt accctgctgt
      241 ggcataaacg gaacagccat aatccgaaga tcgtctggtt acgggaaacc attaaaaacc
      301 tttacgcgtc gatggcataa ccgaatcgta tgaaacgcga cccaatttca caataaaatg
      361 taaaaaagtt gtaataagct tgtctgaatc gaacttttag ccgctttagt ctgtccatca
      421 ttccagtaaa tgattactct tgtattcata atggaccatt aagcatggag cgaaaaatgg
      481 cgactcactt tgcccgaggg attttaacgg aaggacatct gatttctgtt cgtctcccct
      541 cccagtgtca tcaagaagcc cgaaacattc cccctcatcg tcaaagccgt tttctggcgt
      601 ccagaggttt actcgcagaa ctgatgttca tgctgtatgg cattggcgaa ttgccggaaa
      661 tcgtcaccct gccgaaaggt aaaccggttt tcagtgataa aaatttgcct tcgttttcta
      721 tttcctatgc cgggaatatg gttggcgtgg cgttaacaac cgaaggtgaa tgtggcctcg
      781 atatggaact acagcgtgcg acgcgcgggt ttcatagccc acacgcgccc gataaccaca
      841 ccttttccag caatgaatcg ctatggatca gtaaacaaaa cgatcctaac gaagcgcggg
      901 cgcagctcat tacgctgcgc cgaagcgtgc taaaactaac cggtgatgtt ttgaatgacg
      961 atccgcgcga tctgcagctg ctgcccattg ccggacgcct gaaatgtgct catgtaaatc
     1021 atgtagaagc gttatgcgac gcggaggacg tgctggtgtg gtccgtggcg gtcacgcccg
     1081 cgattgaaaa gctcagtgtc tgggagttag atggcaaaca cagctggaaa agcctgccgg
     1141 atattcacag ccgcgccaac aatcctacca gccggatgat gcgttttgcc cagctctcta
     1201 ccgtgaagtc tttttcgcca aattgataga caaccacagg agtcattatg tctgaaaaat
     1261 tgcaggtggt tacgttactg gggagcctgc gcaaaggctc atttaatggc atggttgcac
     1321 gtaccctgcc gaaaattgct ccggcgagca tggaagtcaa tgcgttacct tccattgccg
     1381 acattcccct gtatgacgct gacgtacagc aggaagaagg ttttccagca acggttgaag
     1441 ctctggcgga acagatccgt caggctgacg gtgtggtgat cgtcacgccg gaatataact
     1501 actcggtacc gggtgggctg aaaaatgcca tcgactggct ttcccgcctg ccggatcaac
     1561 cgctggccgg taaaccggtg ttgattcaga ccagctcaat gggcgtgatt ggcggcgcgc
     1621 gctgtcagta tcacctgcgc cagattctgg ttttcctcga tgcaatggtg atgaacaagc
     1681 cggaatttat gggcggcgtg attcagaaca aagttgatcc gcaaaccgga gaagtgattg
     1741 atcagggtac gctggaccac ctgaccgggc aattgaccgc atttggtgag tttattcagc
     1801 gagttaagat ctaaataaaa aacccgccag taatcatgca tggcgggttt ttaatgcgct
     1861 atcgatttta atgagcgtcg ataaatacaa tcttcaggat aaacagcagc gcaacgatga
     1921 ttacgcacgg gctaaggtca cgcagacgcc cggtaccaat cttcatcacg cagtaggaga
     1981 taaagcccag cgcaatacct tcggtaatcg aaaagctgaa cggcatcatc acggcggtaa
     2041 taaacgccgg aacagattca gtaagatcct gccagttcac gcgagcaaga cttgaggtca
     2101 tcaacacgcc aacgtagatc aacgcgccag ctgcagcgta gtctggcacc attcccgcca
     2161 gcggcgacag aaagataacc agcaggaaca gcagaccaac aaccactgcc gtcagaccgg
     2221 tacgaccgcc aaccgatacg ccggaagagg actcaatata agccgtaacg gaagaagtac
     2281 cgataaacga accggtcacg gaagagatac tgtcgacata cagcgcctgc ttcatgcgcg
     2341 ggaatttccc cttctcat
//
LOCUS       DAJTYM010000219         2260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTYM010000219 DAJTYM010000000
VERSION     DAJTYM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.223"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..798
                     /locus_tag="OO575_003999"
                     /pseudo
     CDS             1..798
                     /locus_tag="OO575_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            913..1254
                     /locus_tag="OO575_004000"
                     /pseudo
     CDS             913..1254
                     /locus_tag="OO575_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1237..2074
                     /locus_tag="OO575_004001"
                     /pseudo
     CDS             1237..2074
                     /locus_tag="OO575_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2124..>2260)
                     /locus_tag="OO575_004002"
     CDS             complement(2124..>2260)
                     /locus_tag="OO575_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6666740.1"
                     /translation="RAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS
                     "
BASE COUNT          549 a          549 c          683 g          479 t
ORIGIN      
        1 ctgggccttg cccgtagcag ttatttttat caccgggctg cactgaaagc cggtgataaa
       61 tacgccacga tacgtaagat gctgacagat atctttaaca gtaattacca gtgttatggc
      121 tatcgtcgcc tgcatgcgat gctcaggcat gagggggggc ggctatcaga aaaggttgta
      181 cgcagactta tggtggagga acagcttgtc gtcagtcgta accgtcgtcg tcgctacagc
      241 tcatattgcg gagaaatcgg accggctccg gataacctta tcgccagaga ttttaaggcg
      301 gagcaaccta atcagaaata gctgacagat atcacggagt tccagctccc tgcaggtaaa
      361 gtctggctgt caccagtggt ggactgcttc gatggaaaag ttgtgagctg gtctctcagt
      421 acacgccctg atgctgaact ggtcaacact atgctggata gcgctgtcga aacgttaaat
      481 gctggcgaac gaccggtgat acacagtgac agaggtgggc attatcgctg gccaggctgg
      541 ctggaaagag tgaatgcagc aggtcttatt cgctcaatgt cccgtaaagg atgttcacct
      601 gataatgctg catgcgaagg ctttttcggc agactgaaaa cggaaatgta ttatgggcgt
      661 aaatggtcgg gcatcacgcc agaaaagttc atgcagcaag tggatgctta catcagatgg
      721 tataacgagc ggcgtataaa attatcgctg ggtgccgtca gccccaaaat gtaccgccaa
      781 caatgcgggc tggaatgata aagcagtcca ggaaatcgtc cgcatccccc cttcaccatt
      841 tggattgtcc gctccaccca acatgttgtt tccttaaggt tcctacacca caaaggaaac
      901 tctcatgctg cgcagtggat ggctgacgtt gccgacaggc gggaacttcg ccagttcagg
      961 cagacaccgg aacagcgctt cacgcaggaa caggagcatc tgcagccgct gctgggtacg
     1021 gacttcgata tccgtcatgt ctcctgggat ggctatatcg aggttggcgg gaaccgttac
     1081 agtgtgccgg aaagcctgtg tggtcagctg gtatcaatac ggatctcgct ggatgatgaa
     1141 ctgcggatct acagtaatga gcaacaggta gcatcgcacc gtctctgttc ggctgcatac
     1201 ggctggcaga ccgtgccgga gcatcacgct ccactctggc agcaggcttc ggagcgcatg
     1261 gccgaatggc tgggggagat acagaaacgg gtaatcactg tctgtgaccg ggaagcggat
     1321 atctggcatt acctgtatta taaagtcagc cacggacagc gtggtgcgtg ctgcacagaa
     1381 tcgccggctg gaagaggcac ccggcaagct cttcgaactg ccggaagtcc tggcaaccgc
     1441 aggaagccac acgctgaatg tgatgcaaaa aggcgggcgg gcagcccgtc aggcccggat
     1501 gttcatcagt tacagcgaag tcagcataaa aaatcccgac aacagcggcc aggcgctccc
     1561 gctcacgtat gtctgttgcc gggagcaggc agaggacggt gcctgctggc atctgctgac
     1621 gtcaggaaaa gcggcgagtg ccgccgatgc acgacgtatt gtcagccatt acgagcgacg
     1681 ctggctgaca gaggaatacc acaaggcgtg gaaaagtggt ggtacatgga atcgctgcga
     1741 atgcagaccc gggataacct tgagcgcatg gtggttatcc tggcgtttat agcggtacgg
     1801 gtgctgggcc tgcggcaggg aggtgtcagt gaagaaacac aaaatgacag ctgtgagaaa
     1861 atactgacgc cgacagagtg gaaactactg tgggttaagc tggaaggaaa gccgcttccg
     1921 gtgcaggcac cgaccctgaa atgggcctgg ggcgatggca tgacagcaaa cgcacaggtc
     1981 gtcccggttg gggcgtcatg tgggatggct ggtcagactt caggatatgg ttgagggtta
     2041 cctggtgatg aagtctcttg atcaggagct ctgatcaaga gacagcagct taggggggtg
     2101 cccctattaa gttgtcaagc atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc
     2161 atcatggcga acagaacgtc gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt
     2221 cgttttccct gactcatttt gcgggtgtag taagccctgg
//
LOCUS       DAJTYM010000220         2248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTYM010000220 DAJTYM010000000
VERSION     DAJTYM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            661..2169
                     /gene="ltrA"
                     /locus_tag="OO575_004003"
     CDS             661..2169
                     /gene="ltrA"
                     /locus_tag="OO575_004003"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6666741.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          573 a          555 c          664 g          456 t
ORIGIN      
        1 gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt
       61 aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca
      121 gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga
      181 tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt
      241 taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca
      301 ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga
      361 aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa
      421 gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac
      481 cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg
      541 agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga
      601 atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa
      661 atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg
      721 ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca
      781 aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg
      841 gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc
      901 gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg
      961 ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt
     1021 gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc
     1081 acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc
     1141 gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc
     1201 aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc
     1261 gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg
     1321 ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc
     1381 gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg
     1441 gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat
     1501 tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg
     1561 ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat
     1621 gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc
     1681 gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg
     1741 acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac
     1801 cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc
     1861 agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt
     1921 acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa
     1981 acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg
     2041 ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg
     2101 aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc
     2161 agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg
     2221 cagggaaata gtccgactac gccctgcc
//
LOCUS       DAJTYM010000221         2161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTYM010000221 DAJTYM010000000
VERSION     DAJTYM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..730
                     /locus_tag="OO575_004004"
     CDS             <1..730
                     /locus_tag="OO575_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6666742.1"
                     /translation="KLGDDKGVGEVVGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQ
                     MSDGTTIGYHIGVAYKVDPSKVTTVFQTYRKGVDDITDTDLRQKIADALNRLASKMTT
                     DKFIDGGKSELLDAALKDIQEEMTPIGIQVMSLSYVGKPEYPPTVIDSINAKVTANQK
                     TLQCEQEVKQREAEANMLRAEAAGQADAIRTKAQAEADAIRLRGEALRQNPNVMELEA
                     INKWNGTLPQYMTSGANTPFIQVK"
     gene            774..1523
                     /locus_tag="OO575_004005"
     CDS             774..1523
                     /locus_tag="OO575_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6666743.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            1520..2089
                     /locus_tag="OO575_004006"
     CDS             1520..2089
                     /locus_tag="OO575_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666744.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
BASE COUNT          584 a          549 c          569 g          459 t
ORIGIN      
        1 caaactgggc gacgataaag gcgtcggaga agtggtcggt gttgggcgct actggacggg
       61 atggaacact gaggtttaca tcttcccgac cttcaaacaa atgaagacct acgatgagcc
      121 attcagcttc cagatgagtg acggtacaac catcggctat cacatcggcg tggcctacaa
      181 agttgatcca tccaaagtta ccacagtctt tcagacctac cgcaaaggcg tggacgacat
      241 taccgacacc gacctgcgcc agaagatagc cgacgcactc aaccgactgg ccagcaaaat
      301 gaccaccgac aaatttatcg acggcggcaa gtctgagctg ctggatgcag ctcttaaaga
      361 cattcaggaa gaaatgacgc ccatcggtat tcaggtaatg agcctctcat atgtgggtaa
      421 gccggagtac ccgcctactg ttatcgacag cattaatgcc aaagtcacgg cgaaccagaa
      481 aaccctgcaa tgcgagcagg aagtaaaaca gcgcgaagcg gaagccaaca tgttgcgcgc
      541 ggaagctgcc ggacaggcag atgcgattcg cacaaaagcc caggccgaag ccgatgctat
      601 tcgtttacgc ggtgaagctc tgcgccagaa cccaaacgtt atggagctgg aagccatcaa
      661 caagtggaac ggtacactgc cgcaatacat gaccagcggt gccaacacac catttattca
      721 ggttaagtaa tacatatgcc cggtattaca cgccgggctg tctggagata aaaatgaata
      781 ctgtaaccat caacaacaaa cagtttccgg taatcgaata tcgcggtcag cgtgttgtga
      841 cgctggcgat gattgatgaa gtccaccagc gaccggaagg aaccgcgcgt gcagcattca
      901 accgaaaccg tgagcatttc atcagcggtg tggattatgc cgaattaggt gcggacgtaa
      961 tacgtacgga cctcccggaa ggaacattct ctaaatttgc accatcgggg attgtacttt
     1021 ttgaatcagg ttacctgatg ttgacgaagc cattcaatga cgcccttgcc tggcaggttc
     1081 agcgcgaact ggttaacagc tacttccgca ctcgcacacc gctaacggaa atcgagatga
     1141 tcgctgcgat agccgccgac gctgttcgtc aacagaagcg cctgaatcat gtggaagaac
     1201 agctcgaaac agtcacagaa gcggtggaaa ccatcaaacg cggaaatatg cgcgccggat
     1261 atgtcggtta ccgccaggtg gtagccaaaa gtggaatgag tgacaccaag tgtcggaatc
     1321 tggtgaacgc atatcaaatt cctacagaca cccacgaatt catgactccg gacggtctgt
     1381 tgtctcgcag ggctatcgtc gagcttgagc catttatggc ggcgttccac caaatgatgt
     1441 cagaggctga cccacgcggc acacgctggt atcacccgaa gatggggctt ttccaggtga
     1501 tcggatggga ggataaagca tgatcatcca gtcaaaactt attcgcgccg ctctggtgtg
     1561 cgctgctaaa aacgacgttc gttattacct gaacggtctt cacatcacgc cgaaacatat
     1621 tgaggcaacc aatggttccg tggcactgcg catggctcac ggcatccgga cgaagaaaaa
     1681 catcattgtc cagttcgaag ggggcgtccc ggccaaagcc gaaacgacag agctgatttt
     1741 tagtaaagag ccgatcgctg ttcatcgcga ccagtttcag cgacgactgt ccattaccgg
     1801 cattaaactg gtggacggtt gttttccgga tttggatcgc atcattccga aaaagtttga
     1861 ccgctgtact cacccggtgc tccaggcggg ttacctgagt tatccagaga agatgtttgg
     1921 tcgtgagcgt aaatttattc ccgtccagtt acgtccctcc ggtgacgggc aagcggtcag
     1981 aattcagttt gattccatca tcaactcaat gtatggcaat cctgaatttg ttgtgatgcc
     2041 ttgtcgtgat catggcgatt tcaacgtggc tcaggagcat ccggaatgaa aatcgaatac
     2101 caggacgccg caggaggtga atcaatgagc tggcctgatg caatcgtaac tctggggatg
     2161 g
//
LOCUS       DAJTYM010000222         2076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTYM010000222 DAJTYM010000000
VERSION     DAJTYM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>2076)
                     /locus_tag="OO575_004007"
     CDS             complement(<1..>2076)
                     /locus_tag="OO575_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6666745.1"
                     /translation="SWQFDSDKLQLNISIHQIYMDAMAYDYISPTRWDEGINALTINY
                     DFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGRAEYNSISTWIQ
                     RDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFAPVVRGIAKSNA
                     TVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPYASLAI
                     LKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGV
                     GKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYRYSTEG
                     YYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVE
                     RLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLSGWWGN
                     SYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSY
                     GTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAARLEIDNMR
                     GAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVT
                     HVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSW
                     GENASTKCI"
BASE COUNT          535 a          528 c          458 g          555 t
ORIGIN      
        1 aagatgcatt ttgttgaggc gttttctccc caggataacg taacctgtcc tttttctggt
       61 agaccagaaa gataaagttc gccacggtca ccgacaattc ctgaatctgc caaacttgca
      121 tcttgtatcg cagcaatggc tccaaaaggt accggtttac catttgcatt taacactctg
      181 aagagaacgc gataacccac atgagtcaca aactccgctt taaccactgc accacgcgtg
      241 ggaaccatcg taacgacggt attatcaagt tctatattcg acttcgagaa atagttatcg
      301 ctgattgcta cccgattttc atcataaggg tttagctgtg tcttgaccgt atagccgcgc
      361 cagtccgtcg cagcaccgcg catattatct atttctaacc tggcggcccc aggtgcttta
      421 acaagagcaa tagtttcacc taactcctga cttaacgtta ccccttcgct gtgtgccaga
      481 attccccctc tgataccata attcagcctc tgggaatttt ggctgtagtt atagctgccg
      541 ttcaccgtgc cataagagcc atcataggtc agggacatat taccgccaac ctcatcatca
      601 tgactgttat aggactgcat taaattccat gaaaggttat ttctttccag cgccgtaccg
      661 ctgagtccga gttgatgtga gctaccggaa ttatcgtttt gcgaaacaga ataggtggcg
      721 taactattac cccaccagcc cgataaagga acagataaag agagcatgta gatctgctca
      781 ctggtgttat cactggcatg gttgttcccc gtcccctgtc tggcagtatt ggaataactc
      841 caggagacgt tccacgagag acgcttccag ctactgctgt agccaaattg cattaatcgt
      901 tcgacatcat cggtatgcca gtattgttgc cggcttaatg tcaggtataa attgccataa
      961 tctctcccca acgactgcgt tagcgttccc tccacgcgac tacgtcggtt acctgtttcc
     1021 caaaagtctt cagggctgtt gcgccgcgat gcccactcat tgagggtata gtagccctcg
     1081 gtggagtaac gatagccaac caggcgcaag ctagtgtctg tgtcgtcaaa tagttttgaa
     1141 tagagaaagc gatatgactg acccgtttct gtatcatcat ggctaaaatt cgcacgagca
     1201 tgtgtcacat cgaaagaaat tgcgcccaaa gcgccaagat ctttaccgac acccagagct
     1261 gcagaaccat aattttcagc agcctgcata cctccatata aagtgatccc gtgggaaaaa
     1321 ccatgaagta tttgcgcctg aaggacgtcc ggtgtaaatc cgtcttcatc gcgcaattca
     1381 cccacgctga catcaacatc tgtcagacct tcacgtttaa gaatcgccaa tgaagcataa
     1441 ggttgggtga atcgttgttc gctaccgtct tcttctttga tggttacgtc caaatcgcca
     1501 cctgtacttg cggtgttgag atcggtgatc tcaaaagcac cttgtggaac ggctgactga
     1561 taaatcacgt agccattctg ccgaatgatg acggtggcgt tggactttgc aatcccacga
     1621 accacaggag caaagccatt ctggctggcg ggtagcatat cgttatcagt atacaaacgc
     1681 gcgccgcgaa tttgcgtact gtcgaaaata tcgctcgccg tccacgtatc accaatcata
     1741 atctggcttc ttaacgcggc aatatcgcgc tgtatccagg tactaatgga gttgtattcc
     1801 gcacggccat cgctggtgtt taaagtactg taattacgta gccgccacgg tccaatattc
     1861 agtccattgc gcagattgag ataactggta tctgtctctt gtgaaccata atctgaacgt
     1921 agtgtatgtg aaccagaaaa atcgtagttg atggtgagcg cattaatccc ctcatcccaa
     1981 cgcgtggggc tgatgtaatc ataagccatc gcgtccatgt agatttggtg aatggatata
     2041 ttcagctgca atttatcact gtcaaactgc caggat
//
LOCUS       DAJTYM010000223         2022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTYM010000223 DAJTYM010000000
VERSION     DAJTYM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(210..374)
                     /locus_tag="OO575_004008"
     CDS             complement(210..374)
                     /locus_tag="OO575_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666746.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1276..1560
                     /locus_tag="OO575_004009"
     CDS             1276..1560
                     /locus_tag="OO575_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6666747.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1548..>2022
                     /locus_tag="OO575_004010"
     CDS             1548..>2022
                     /locus_tag="OO575_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6666748.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLK"
BASE COUNT          520 a          423 c          582 g          497 t
ORIGIN      
        1 ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga gccggttact
       61 ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt ctgcaatacc
      121 ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata gctgatggag
      181 ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc gtttccggcg
      241 taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt atgagagatg
      301 aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt aagagcggtg
      361 gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt gcggagatga
      421 acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc gaaaatgtgc
      481 tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc ggcatccggc
      541 atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc tgtcgtctcg
      601 atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg atgcgagaca
      661 caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg agcgaagggg
      721 gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc catgagtcag
      781 ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg ggtcaaagct
      841 aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt taccaatcga
      901 agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt gcccgcaaac
      961 acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga attcgtcacg
     1021 actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg gttcaacgac
     1081 aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg ggggcggtgc
     1141 ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta aatgccgctg
     1201 acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg tcaatatgct
     1261 attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca gctcaatctc
     1321 agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct tcataagtcg
     1381 cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat tctcgaccgg
     1441 cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct tgatgctccg
     1501 gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg ggatgtgtaa
     1561 ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc agtgaagctg
     1621 tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga gccacccgga
     1681 cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca ctggccactg
     1741 gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca gaccccattc
     1801 ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa gggcttggcg
     1861 cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat atcggtgtgc
     1921 gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt catcatggtt
     1981 ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa ac
//
LOCUS       DAJTYM010000224         2015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTYM010000224 DAJTYM010000000
VERSION     DAJTYM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..628)
                     /gene="nanT"
                     /locus_tag="OO575_004011"
     CDS             complement(<1..628)
                     /gene="nanT"
                     /locus_tag="OO575_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6666749.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAP"
     gene            complement(738..1631)
                     /gene="nanA"
                     /locus_tag="OO575_004012"
     CDS             complement(738..1631)
                     /gene="nanA"
                     /locus_tag="OO575_004012"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6666750.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(1753..>2015)
                     /locus_tag="OO575_004013"
     CDS             complement(1753..>2015)
                     /locus_tag="OO575_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCR6666751.1"
                     /translation="VLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVSYQQ
                     HIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT          498 a          538 c          523 g          456 t
ORIGIN      
        1 ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc ttccgggatg tttttacgca
       61 gccagagagc aaagatgatt ggcaaaatgc cgataaagaa cagcgcacgc caaccccaga
      121 ccggaaccac caggctatag acctgagcgg caacgacggc ccccacagag aagcctgaaa
      181 tcaaaaaacc actggctttg ttacgcagat gttttggcca gctttcaatg acataggtgg
      241 cgctggaacc gtattcaccc gccatcccca tgccgatgac cagacgagcg ataaacatgg
      301 tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc cgagaagaga acgatgctgg
      361 tgaccattgc cagacgacgc ccgtagcggt cacccatagc gccgagcatc aggccgccga
      421 accagcgaga gataaaggct gcggagatca gacttgccgc ctgcaccgtc gtcagcccga
      481 attcaccctg tacttcggtg agtaccaggg cgattaaaac gaaatcaaaa ccgtcaagca
      541 gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc acggttgaga tggcgatacc
      601 acgggatatt ctgggttgta gtactcattg tgagtctccc gcggtgggcg atgcccacac
      661 gctttggtat gaaaattgta gggtacagat gcgtttattt cccctcaccc ggtaggggcg
      721 agcgagggga aaacaactca cccgcgctct tgcatcaact gctgggccag cgccttcagt
      781 tctggcagat atttttcatc taccggtcca aacggtttgc ggcacagcgg cacagaaacg
      841 acatccatat aatgcaggac agttttcagg ccgcggaata cgcccgtttt gatcagtaaa
      901 tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg tctggatatc gccttctttc
      961 agcgccttca cgatcccctg atatcgccag cccatgatgt tgtaagtact gccgatacca
     1021 ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt cgtaaccgtt atagagcacc
     1081 agatcaggat gttcacgacg gatctgttcc atctgataga gatcgccaga agtctgtttc
     1141 agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct gatccagggt cagttttacc
     1201 ccactcaggg ctggaatgtt gtacaccacc atcggcaaac catccgctga atcaataatt
     1261 gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat agtagaacgg cgtgacggcg
     1321 gagacggcat cgaagccata acgtttagcc gatgccgcaa gttgttggct ttcggcggtg
     1381 ctgacgcaac cgacgtgggc gatgagttta atcttacctt tcgcctcttc ggcgacgatt
     1441 tccagtacct gttcacgctc ggaaaggctt tgtacaaagg cttcgccggt cgaaccaccc
     1501 acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa ccaggcggcg cagactcgct
     1561 ttatccagtg cttgttgttg atcaaaagga gtcaggagtg cagccattac gccacgtaaa
     1621 ttcgttgcca taaatacctc tgaagtgatg cttgtctgat aaacgatata cctttatacc
     1681 tgttatacca gatcaattaa gcaacacccc atacagaaag cttataatgc gatctgcttc
     1741 actaaagtgg cattatttct ttttgttggt ggtctgaccg aaagcgtgcc aggtagcaga
     1801 gacgctgttg agatgcgatt gcaacgcacg atcggcttcg tcaggatcat gacggcggat
     1861 cgcatcaacg atcgcaatat gctgttgata actaacgttg ttatgttcgt gcagtgcctg
     1921 atcggtaacc gttgggcgtg cggcaataag ccagtcgagc agggcaacgt ggatcgccat
     1981 gaagattggg ttaccgggga tctccgccag cacgc
//
LOCUS       DAJTYM010000225         2007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTYM010000225 DAJTYM010000000
VERSION     DAJTYM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            166..470
                     /locus_tag="OO575_004014"
                     /pseudo
     CDS             166..470
                     /locus_tag="OO575_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            639..2003
                     /locus_tag="OO575_004015"
     CDS             639..2003
                     /locus_tag="OO575_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6666752.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
BASE COUNT          511 a          400 c          465 g          631 t
ORIGIN      
        1 acgatagcca aattacctat acccatcccg gccctgccaa gccatacccg ctatcggcga
       61 tgaaaatgct tttgtgcgcg gtcggagtgg tcccgacgag ggttttaccc gaagtcgggg
      121 cgtatctccg cgttagcggg ccgtgagggc cgcttacgag cgtgtatgag agacgtacag
      181 tggttttgtc cgctcacacc agtacataca ggtacggaaa taaaaccagt cgaaatgccg
      241 gtaacgacga ttactcctgt ctctgatgcc ggtggactac aagattttat ttactggcgt
      301 cctgatgcgg acagggctgg tatggaacct gtttatgtga tacttaatga tcctctggat
      361 tccgggcgat ttacgcgtaa acaactaaat aaaaatatct taaacatgca agtaattttg
      421 gtattctcga tacaagaata aatagtagta actactatag ctgcaaataa tgttaaaaac
      481 atttctatga tcatctttac atttttcatg attgacaatt gtttttggtt aaaaaaagac
      541 aactaatgag tgtgataatg caatactttg agtgattata taatgataag taaacaggtt
      601 tgcgacaatt ggttatcagt attacagaag gattaaatat gtcattattc acagaaccta
      661 aggaaattga aagattaccc tctgaagaga ttgaacgatt atatcccgtc ctgcgataca
      721 gggtctttat cagcattttc ctcggatata tggggtatta ttttgtccga aacaccacca
      781 gtgtgttatc cggtgtgctg cacatgtctg caaccgagat cggcattatc tcctgtgccg
      841 gttttttatc ctatggtata tcaaagtttg tttccggact tatttctgac agaagtaatt
      901 caaaagtatt tttgtctctt ggtttattcc tgtccggact ggtaaatttt ctgataggat
      961 atattcccgg gatcatcaca tccgttacgt tattttcaac aatgtattta cttaacggct
     1021 ggatccaagg tatgggatat cctcctggtg ccaaaacact ggtgttctgg tatgaacatc
     1081 gggaaaggat cacctgggcc acactgtgga atttatcgca caatgtcggg ggagctctgg
     1141 cccccgttct gattggtttc agttttggtt ttttcggcga cagtgctctg gatcatgccc
     1201 gggccgcatt tatcttccca ggagtactgt gtatggcaat gagtgtgctg atttacttta
     1261 ttcaggtgga cagacctgta tcagtggggc ttcctcccat tgaagaatgg aaaggtaacg
     1321 tcgtttcaca cccggctaaa ggccgtgaac aaggtccgag actctccatt cctgatatca
     1381 tcagaaaaca tatcatccga aataataaac tcatttactg ctgtatctat ggttcgtttg
     1441 tttatattct gagatacggt attgtttcct gggctcctaa gtttttaagt gacagtctgg
     1501 atgtcggtgg taaggatatg ggaaaactgg catcaatggg ggggggttct gtttttgaaa
     1561 tcggtggtgt ggctggtatg cttcttgccg gatacctgtc tgtcagactg ttcagaaact
     1621 caaaaccatt aacaaatact ttatttctgg cacttaccat tattttatta atcgcgtact
     1681 ggtacgtgcc atcaggcaat gaatatctct ggctaaacta tacaatcctt attctgttgg
     1741 ggctggcagt gtatggtccg gtgatgttta ttggtcttta ttcaatggag cttgttccta
     1801 aagaagctgc cggagccgca tcagggctgt caggcacatt ttcctatatc ttcggctcaa
     1861 ttgttgcgac actgggaatg ggacttgttg tcgattatct gggatggggg gccacattta
     1921 tcgtactgat actgtctgct gtttttgcca tcattttcac actgatgagt cgtgaacgct
     1981 cactggagtt cgaaaaagag taatttt
//
LOCUS       DAJTYM010000226         1998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTYM010000226 DAJTYM010000000
VERSION     DAJTYM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..932
                     /gene="efeO"
                     /locus_tag="OO575_004016"
     CDS             <1..932
                     /gene="efeO"
                     /locus_tag="OO575_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6666753.1"
                     /translation="WEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMTCGLLTNPK
                     GKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDAIKAGDIEK
                     AKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHRLEKALFGD
                     NTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKISGEEDRYS
                     HTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKYRTKDGFET
                     YDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            938..>1998
                     /gene="efeB"
                     /locus_tag="OO575_004017"
     CDS             938..>1998
                     /gene="efeB"
                     /locus_tag="OO575_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6666754.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGV"
BASE COUNT          517 a          481 c          581 g          419 t
ORIGIN      
        1 agtgggagat cctcaaaggc gtgatggtgg tggaagagcg ggaaaatatc gcccctggtt
       61 ttagccagaa aatgacggcg aatttacagc ctggcgaata cgatatgacc tgcggtctgc
      121 tgactaaccc gaaagggaag ttgatcgtca aaggtgaggc aacggcggat gcggcgcaaa
      181 gtgatgcgct gttaagtctt ggtggtgcaa ttactgcata taaagcgtat gtcatggcgg
      241 aaaccacgca gctggtgacc gacaccaaag cctttaccga cgcgattaaa gcaggcgata
      301 tcgaaaaagc gaaagcactg tatgcgccga cgcgccagca ctatgagcgt attgaaccga
      361 ttgctgaact gttctccgat ctggatggca gcattgacgc ccgtgaagat gattacgagc
      421 aaaaagccgc cgacccaaaa ttcactggtt tccaccgtct ggaaaaagca ttgtttggcg
      481 acaacaccac caaagggatg gatcagtacg ctgagcagct ttataccgat gtggtcgatt
      541 tgcaaaaacg catcagtgaa ctggctttcc caccttcaaa agtggtcggc ggcgcagccg
      601 gactgattga ggaagtggca gccagcaaaa tcagcggtga agaagatcgc tacagccaca
      661 ccgatctgtg ggatttccag gctaacgttg aaggctcgca gaaaattgtc gatctgctgc
      721 gtccacaact gcaaaaagcc aacccggaac tgttggcaaa agtcgatgcc aactttaaaa
      781 aggtcgatac cattctggcg aaataccgta ctaaagacgg ttttgaaacc tacgacaaat
      841 tgaccgatgc cgaccggaat gcgctgaaag ggccgattac tgcgctggcg gaagatctgg
      901 cgcaacttcg cggtgtgctg ggactggatt aagcgttatg cagtataaag atgaaaacgg
      961 cgtgaatgaa ccgtcacgcc gacgtttact gaaagggatt ggtgcgctgg cgggaagttg
     1021 tccggtcgct catgcacaaa aaacgcaaag tgcaccgggc acgctttcac cggatgcacg
     1081 caatgagaaa cagccgtttt atggtgagca tcaggcaggg atcctgacgc cacaacaggc
     1141 cgcaatgatg ctggtggcgt ttgatgtgct tgccagcgat aaagccgatc ttgagcggtt
     1201 gtttcgcttg ttgactcagc gttttgcttt tctgagtcag ggcggagcag caccagaaac
     1261 gccaaatccg cgcctgccac cactcgattc cggcattctt ggcggctaca ttgcgcccga
     1321 taatctcacc atcacgttat cggtgggtca ctcattgttt gatgagcgct ttggccttgc
     1381 gccacagatg ccaaaaaagc tgcagaagat gacgcgtttc cccaacgact cgctggatgc
     1441 ggcgttatgt catggtgatg tgttgctaca gatttgcgcc aacacccagg acacggtgaa
     1501 ccatgcgctg cgcgatatca tcaaacacac gccggatttg ctcagtgtgc gctggaagcg
     1561 ggaagggttt atttccgatc acgcggcgcg tagtaaaggc aaagagacgc cgattaattt
     1621 gctgggtttc aaagacggca ctgccaatcc cgatagccag aatgataagt tgatgcaaaa
     1681 agtggtgtgg gtaacggcag atcagcagga gcctgcgtgg acaatcggtg gcagctatca
     1741 ggcagtacgc ttgattcagt ttcgagtgga attttgggac agaacgccgc tgaaagaaca
     1801 gcagacgatt tttggccgtg ataagcaaac cggtgcgccg ctgggaatgc agcatgagca
     1861 tgatgtgcct gattacgcca gcgacccgga agggaaggtg atcgcgctgg acagccatat
     1921 ccggctggcg aatccccgca cggcggagag tgagtccagc ctgatgctgc gtcgcggcta
     1981 cagttattcg ttgggggt
//
LOCUS       DAJTYM010000227         1985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTYM010000227 DAJTYM010000000
VERSION     DAJTYM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(64..822)
                     /locus_tag="OO575_004018"
     CDS             complement(64..822)
                     /locus_tag="OO575_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6666755.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO575_004019"
     CDS             complement(830..1933)
                     /locus_tag="OO575_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6666756.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
BASE COUNT          546 a          545 c          436 g          458 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatc
//
LOCUS       DAJTYM010000228         1971 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTYM010000228 DAJTYM010000000
VERSION     DAJTYM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..769
                     /gene="lsrF"
                     /locus_tag="OO575_004020"
     CDS             <1..769
                     /gene="lsrF"
                     /locus_tag="OO575_004020"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR6666757.1"
                     /translation="QSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAPLFE
                     HADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCAV
                     AAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAE
                     MGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMGR
                     NIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            793..1083
                     /gene="lsrG"
                     /locus_tag="OO575_004021"
     CDS             793..1083
                     /gene="lsrG"
                     /locus_tag="OO575_004021"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6666758.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1123..1881
                     /gene="tam"
                     /locus_tag="OO575_004022"
     CDS             1123..1881
                     /gene="tam"
                     /locus_tag="OO575_004022"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6666759.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
BASE COUNT          481 a          457 c          542 g          491 t
ORIGIN      
        1 gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga tgctggcctt
       61 tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata taaatatcgc
      121 cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc gcagcgtagt
      181 tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact ctattctggc
      241 ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc tgaacagttg
      301 cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga tcaaaaatat
      361 tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg tgactggcgt
      421 gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa tcgccgctga
      481 aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac ggattgttgc
      541 cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc gcgagacgct
      601 ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg ggcgtaatat
      661 tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg ttcaccataa
      721 cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac tgcggattta
      781 aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga caaggttgac
      841 gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga aggtaatttg
      901 cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta cgaagcctat
      961 aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac ctgtgtcgcg
     1021 aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg gttgatgccg
     1081 tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga ctggaacccc
     1141 tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct gcttgccaga
     1201 gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg taacagcacc
     1261 gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc gtctccggcg
     1321 atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc agacatccgc
     1381 aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact gcaatggctg
     1441 cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca gggcgtgctg
     1501 gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg cgaagttgct
     1561 tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca tgcttactac
     1621 gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta ctatcaccag
     1681 atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg tccgtggtta
     1741 caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca gatgctggaa
     1801 gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg tctgtttatt
     1861 gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg gaagaatttt
     1921 tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag c
//
LOCUS       DAJTYM010000229         1947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTYM010000229 DAJTYM010000000
VERSION     DAJTYM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..576
                     /gene="proB"
                     /locus_tag="OO575_004023"
     CDS             <1..576
                     /gene="proB"
                     /locus_tag="OO575_004023"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6666760.1"
                     /translation="ADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQA
                     ADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEI
                     TVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDALR
                     RIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            588..1841
                     /gene="proA"
                     /locus_tag="OO575_004024"
     CDS             588..1841
                     /gene="proA"
                     /locus_tag="OO575_004024"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6666761.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          465 a          493 c          566 g          423 t
ORIGIN      
        1 gctgatccgc gcagaaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
       61 gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtact
      121 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
      181 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
      241 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt
      301 gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg
      361 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
      421 aacctcgaag gtcgcgatat cgcccacggt gtcagtcgtt acaacagcga tgcattacgc
      481 cgtattgccg gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg
      541 gttgccgttc accgtgatga catgattacc cgttaaggag ctggccgatg ctggaacaaa
      601 tgggcattgc cgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa
      661 atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg
      721 ctaacgccca ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc
      781 gtctggcact gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca
      841 acctcgccga tccggtgggg caggtaatcg atggcggcgt actggacagc ggcctgcgtc
      901 ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg
      961 tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgtggtg
     1021 gtaaagaaac ctgtcgcact aacgcggcaa cggtggcggt gattcaggac gccctgaaat
     1081 cctgtggctt accggcgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca
     1141 gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt
     1201 tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt ataggcgtat
     1261 gccatattta cgttgatgaa agtgcagaga tcgctgaagc attaaaagtg atcgtcaacg
     1321 cgaaaactca gcgtccgagc acatgtaata cggttgaaac gttgctggtg aataaaaaca
     1381 tcgccgatag cttcctgccc gcattaagca aacaaatggc ggaaagcggc gtgacattac
     1441 acgcagatgc agctgcgctg acacagttgc aggcaggccc tgcgaaggtg gtggcggtta
     1501 aagccgaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagcg
     1561 atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc
     1621 tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt
     1681 acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggtctgggt gcggaagtgg
     1741 cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt
     1801 acaagtggat cggcattggt gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa
     1861 aagtagccat ttgattcaca aggccattga cgcatcgccc ggttagtttt aaccttgtcc
     1921 accgtgattc acgttcgtga acatgtc
//
LOCUS       DAJTYM010000230         1863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTYM010000230 DAJTYM010000000
VERSION     DAJTYM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            146..1759
                     /gene="mppA"
                     /locus_tag="OO575_004025"
     CDS             146..1759
                     /gene="mppA"
                     /locus_tag="OO575_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6666762.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          524 a          411 c          458 g          470 t
ORIGIN      
        1 tatttacact aaatctgatt tgatatattg atacttaaaa catttgatgc ttcctttgtc
       61 acttttttga tggaagttgt ttgcatttct ttaaggcgaa acaaataatt acgcatcaat
      121 tttaatgtcg gttagaggga aacttatgaa gcactctgtt tcagttacgt gttgtgcgct
      181 gttggtcagc agcatttctc tttcgtatgc tgcagaagtt ccgagcggca cagtactggc
      241 agagaagcag gagctggtgc gccacattaa agatgagcct gcgtcgctgg atcccgctaa
      301 agccgtgggc ctgccagaga ttcaggtcat tcgcgatctg tttgaaggtc tggtgaatca
      361 gaacgaaaaa ggggagattg tccccggcgt cgcgactcaa tggaaaagta atgacaaccg
      421 tatctggact tttaccctgc gcgataacgc aaaatgggcg gatggcacac cggtaacggc
      481 gcaagatttt gtctacagct ggcaacgtct ggtggaccca aaaacattgt cgccatttgc
      541 atggtttgcc gcgctggcgg gaatcaacaa cgcacaggcg attattgatg gtaaagctac
      601 gcctgatcag cttggcgtca ccgcagttga tgcccatact ttgaaaattc agcttgataa
      661 accgttgccg tggtttgtga atttaaccgc taactttgcc ttcttcccgg tgcaaaaagc
      721 caacgtagaa agcggtaaag agtggacgaa acccggaaat ctgatcggca atggcgctta
      781 tgttcttaaa gagcgcgtag tcaatgaaaa actggtcgtg gtaccgaata cccattattg
      841 ggataacgcc aaaacggtgc tgcaaaaagt gaccttcctg ccaattaatc aggaatccgc
      901 agccactaag cgttacctcg cgggggatat tgatatcacc gaatccttcc cgaaaaatat
      961 gtatcagaag ctgttgaagg atattccggg gcaggtttat acgccaccgc agctcgggac
     1021 ctattattat gcctttaaca cgcaaaaagg gccgacggca gatcagcgcg ttcgcctggc
     1081 attaagtatg acgatagatc gccgcctgat gaccgaaaaa gtgttgggga cgggcgaaaa
     1141 gccagcgtgg cattttatac cagatgttac cgccggattt acgccggaac cttcgccgtt
     1201 tgaacaaatg agtcaggaag aactgaatgc gcaggcaaaa actttgttga gcgcagctgg
     1261 ttatggtccg caaaaaccgc tgaagctgac gcttttgtat aacacttcag aaaaccatca
     1321 aaaaattgcg attgctgtag catcgatgtg gaaaaagaac cttggcgtag atgttaaatt
     1381 gcaaaatcag gaatggaaaa cctatatcga tagccgtaac accggcaatt ttgatgttat
     1441 ccgcgcctca tgggtggggg attataatga accctccact ttcctgacat tattaacgtc
     1501 aatgcattca ggaaatattt cacgctttaa caatccggca tacgacaaag ttctggccca
     1561 ggcatcgacg gaaaataccg ttaaagcgcg taatgccgat tacaacgcgg cagaaaaaat
     1621 cctcatggag caagcaccga ttgcaccaat ttatcaatat accaatggac gattaatcaa
     1681 accgtggctg aaaggttatc ccattaataa ccctgaagat gtagcgtaca gtcggactat
     1741 gtatattgtg aagcattgat gtgatgggaa ctggcgttac ccttgtggat aacgccagtg
     1801 atgtctgatt tagcgcccct gttccggcgg tgtaatgtta tccatataca gcgtctggct
     1861 ggg
//
LOCUS       DAJTYM010000231         1859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTYM010000231 DAJTYM010000000
VERSION     DAJTYM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..420)
                     /gene="bglB"
                     /locus_tag="OO575_004026"
     CDS             complement(<1..420)
                     /gene="bglB"
                     /locus_tag="OO575_004026"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6666763.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGW"
     gene            complement(439..>1859)
                     /gene="bglF"
                     /locus_tag="OO575_004027"
     CDS             complement(439..>1859)
                     /gene="bglF"
                     /locus_tag="OO575_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6666764.1"
                     /translation="FFPIILGYTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKAD
                     APGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVI
                     TPVTFLLVGQLSTWISEMIAAGYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLC
                     INNFTVLGYDTMIPLLMPAIMAQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPA
                     VYGVNLPRKYPFVIACISGALGATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVW
                     ASVIGGVIAIGCAFVGTLMLHFITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEI
                     VPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEIL
                     IHVGIDTVKLDGKFFSAHVNVGDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDF
                     TDVLPHGTAQISAGEPLLSIIR"
BASE COUNT          491 a          513 c          451 g          404 t
ORIGIN      
        1 ccaaccgccg tagtttttca ccagcccata tagcatttcg taatgggata acgttaccag
       61 cggcttgatc cccgcctgcg ccatttcatc aaacagccga tcgtaaaacg ctaaccccgc
      121 ttcattcggt tcggcttcgt cgccctgagg gaaaattcgc gcccaggcaa tggaaatacg
      181 cagacaggtg aagcccatct cggcaaataa cgcgatatct tccgggtaac ggtaataaaa
      241 atcgatggcg acatctttga tattctcttt ccccaggatg cgcggttcca tttttcccat
      301 tacgccatga ggctgtaaat ctgaggtcgt gatccctttg ccatcttcca gccaggcacc
      361 ttccacctga ttggcagctg ttgcgccacc ccaaagaaat gtttctggaa atgctttcat
      421 aattaactcc ttttatcgtt agcgaatgat ggataacagc ggttcacctg cgcttatctg
      481 cgccgtgccg tggggtaata cgtctgtaaa atcatcgcta ttactgatta ataccggcgt
      541 cgtcagatca aatccggcct cgcgaatagc agggatatca aaagaaatca gccgatcgcc
      601 tgtattgacc ttgtcaccca cgttgacgtg agcggaaaag aatttgccgt ccagttttac
      661 ggtgtcgata ccgacatgaa tcaggatctc cacaccatca tctgactcaa tgccaatggc
      721 gtgtaatgtg gcgaacaacg aagcaattcg acccgcaacc ggagaacgca cttcaccaac
      781 cgagggcaga atggcgatac ctttacccaa caggccactg gcaaacgtgg tatcagcgac
      841 gtgaatgagc ggcacaatct ctcccgtcat cggtgaacag ataccgccct gctcaggtgg
      901 tgtaataacc tctggtgttt tctcttgcgg ggcaccctgc gctggctgac gtttactggt
      961 gatgaaatga agcatcaacg taccaacaaa tgcgcaacct atggcaatga caccgccaat
     1021 aacgctggcc cagacggtga aatcaatccc cgttgacggg atggtctgca tgaaggtgaa
     1081 aatacttggc aaaccaaagg agtaaacttt cgtttgcgcg tagccaatga tggtggcccc
     1141 caatgctcca ctgatacagg cgataacaaa ggggtactta cgcggcaggt tgacgccata
     1201 taccgctggt tcggtgatac caaacagacc cgtcaacgcc gctgatcccg ccaccacttt
     1261 tttctgcgca tcgcgttcgc agaggaagac gccgagcgcc gccccgacct gcgccataat
     1321 ggcgggcatt aacagcggga tcatggtgtc gtagcccagc acggtgaagt tattgataca
     1381 cagcggcacc aggcccctgt gcagtccgaa catgacgaag atttgccaga agccgcccat
     1441 taccgcgccc gcaaatgcag gaaccgcctg ataaagccag agataaccgg cggcaatcat
     1501 ttcgcttatc caggttgata gctgccccac cagcagaaag gtgacgggtg tgataaccat
     1561 cagacatagc aatggtgtga agaaattttt gattgccgac ggtaaccacg cattaagtcg
     1621 gcgttccaga atgctgcaca accaggcaga aaaaataatg ggaataaccg atgacgagta
     1681 attcaacaat gtgaccggaa tacccaggaa atccagcccc ggcgcatccg ctttttgccc
     1741 gttctcgaag gcagtcagaa ttaatggatg cactaacgct ccaccaatca ccatggcagt
     1801 aaatggatta ccgccgaagc gtttgcccgc ggtgtatccc aggattatcg ggaagaacc
//
LOCUS       DAJTYM010000232         1731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTYM010000232 DAJTYM010000000
VERSION     DAJTYM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..184)
                     /locus_tag="OO575_004028"
     CDS             complement(<1..184)
                     /locus_tag="OO575_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6666765.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFL"
     gene            complement(231..950)
                     /gene="yehT"
                     /locus_tag="OO575_004029"
     CDS             complement(231..950)
                     /gene="yehT"
                     /locus_tag="OO575_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6666766.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(947..>1731)
                     /gene="btsS"
                     /locus_tag="OO575_004030"
     CDS             complement(947..>1731)
                     /gene="btsS"
                     /locus_tag="OO575_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6666767.1"
                     /translation="HPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNRLFSSINRTLGE
                     GIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQAS
                     QLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELSQQ
                     QLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGLYQPVTNASG
                     LGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          422 a          464 c          395 g          450 t
ORIGIN      
        1 tgaggaatga cgacaaaaca atgtccggac aacgctgaaa accctcttcg tcttctttac
       61 gtagccagac ggcgatctgt tccgcggtgg cttcgacatt atccagcgcc agaatacgca
      121 ccaggtcatt attattggct ttcaaaatgt agcgcacgct gcgcagaata tcgttactta
      181 tcatgaggcc ttcaggtgtt gatgaggcaa aaagccattt tagcagtctt ttacaggcca
      241 atcgcctctt ttaagctttt cagataacgg cggctgaccg gcacggttaa gccattacgc
      301 aaaatcaact cggcctggcc gttatcttcc agacgaatct cctgtaaatg cgcgaggtta
      361 accagatact gacgatggca gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc
      421 aattcggtaa agccctcttt cccttcgtgg ctggtaacgt agacaccgct catccgactg
      481 ctgacaaatg ccacatcttt catttgcagc aaataaatcc gactatgccc cgtacaaggg
      541 ataaatttca gcgcctgttg attttccggt aacagcgaaa catcctgctt gctgcgctcc
      601 tgacgcaatc gcgccagcgt tttctccagt cgcgcttcat caattggctt cagcagataa
      661 tcaaaggcat gttcttcaaa ggctttaata gcgtattcgt caaacgcagt gagaaaaaca
      721 atatacgggc gatgttccgg gtcaagcatc cccaccattt ccagaccact gatgcgcggc
      781 atctggatat cgagaaacag cacatccggg cgcagtttat gcaccgcgcc gatcccttcc
      841 acggcgtttg aacactctcc aacgatttca atatcgctct gctcctgcaa aaatatacgc
      901 aggttctccc gtgctaacgg ttcatcatcg acaattaaga ctttaatcat gcctcgtccc
      961 tccatggtag tcgtaacgtt attcgggtgt aactatcagg ctcacaggcg acgcttattc
     1021 catagtcatc gccaaaccgt tcacgtaaac gcttatccac cagattcatc cccagcccac
     1081 tggcattggt taccggttga tacaaaccgg cattgtcttc gatctccagc atcaaatgtt
     1141 gcccctcacg tcgggcgctg attgccactc gccctgtatc cagcagttgt gatgtcccat
     1201 gtttaatggc gttttccact atcggttgca gggtaaacgc gggcaattgc tgctgggata
     1261 attcttgcgg aatagcaatg ttgacctgca accgcgactg gaagcgcgcc ttttcaattt
     1321 gcagataagc gttcacatgt tcaatttcgt cggcgagagt aacaaactcc gaaggccgct
     1381 ttaagttttt gcggaaaaaa gtggaaagat actgcaccag ctggctggcc tgttcgctgt
     1441 cgcggcggat caccgcttta atggtgttaa gcgcattaaa caaaaaatga ggattcacct
     1501 gggcgtgaag cagtttgatt tctgactggg tgagcatcgc tttttgccgc tcatattgcc
     1561 ctgcaagaat ctgcgccgaa agcagttgcg caatcccctc gcccagcgtg cggtttattg
     1621 aactgaataa ccggtttttg gcttcataca atttgatggt gcccatcacg cgctgatttt
     1681 cgccacgcaa cggaattacc agcgtcgacc ccagtttgca ttgcggatgc a
//
LOCUS       DAJTYM010000233         1726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTYM010000233 DAJTYM010000000
VERSION     DAJTYM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(74..583)
                     /locus_tag="OO575_004031"
     CDS             complement(74..583)
                     /locus_tag="OO575_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6666768.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            746..991
                     /locus_tag="OO575_004032"
     CDS             746..991
                     /locus_tag="OO575_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6666769.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            966..>1726
                     /locus_tag="OO575_004033"
     CDS             966..>1726
                     /locus_tag="OO575_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6666770.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQR"
BASE COUNT          454 a          372 c          416 g          484 t
ORIGIN      
        1 cagctcatgc cgtttaggta tgttacatgt gtgattatgt gaggtgaagt atgttttagc
       61 tggctcatgg ttgttatacg gcttttttcg catcttgtgg ttccaacaaa ggagctacag
      121 gctcttcctg tttatgaaca ccatacattg acaaaatctc ttggcgagtt tctggaggta
      181 atttatgcag cgccaaagct gtttgtagtg tttggcgatc aatacccagc gttgccaata
      241 gggtgtcaac ggagatggag gggcgttctt gcattaatcc ggaaagccca tatttagcca
      301 tgtttagctc tatctctcgg acttcatttt ctcctagttt attgattaac agggagaaca
      361 ttttcaatgt atcagcattc atttcttcga tagcaatttt ttgcatagcc ggtattgccg
      421 ggatatattc aaccccaggc ccgcgacgcc cttcttgtga acgcttcggc catccttctt
      481 tctttgctcg tttatgcacc ccctgtattg tcccaggcat tccatccagt tcagtaattt
      541 gtttgggtgt catccactgg tttatctcat ttgcgctttt cataaactaa gtttaccaga
      601 tttatctata taaaaatcaa tggattacaa aataaaccac attaactgat ataaaccggt
      661 ttggtttatt tggtaatgta taaaacagat aatcaagacg aattatttgt tttggtaacc
      721 ctttatcata cgcaatatgg ttggtgtgcg aaagatgagt aacgattggc atcgggccga
      781 cgtgattgcg gccctcaaaa aaacaggaac ccctctggca aaattgtcca gacgttcagg
      841 ttattcaacc gggacgcttg caaatgcatt agtcaggcca tggcctaaag gtgaacgcat
      901 tattgctgac gccttaaaca tgaaacctga ggaaatttgg cctagtagat atctaaggga
      961 tcacaatgag agagtggata tcagcaattg aatgcatggg tttatctggt ttacctggtt
     1021 cattaatggg ggttcacaaa agagctcaga gagaaggatg gctcaagaga agaagagaag
     1081 gaattagagg ggttgcttac gaataccata tatcttctct tcctaacatt gctcgagctg
     1141 aagttttgct ccaacaagga aaatttaaaa ccagtcaggg atgttttgaa atcgcccgcc
     1201 ccacgctgga agctcatgat tatgatcgtg aggcactatg gagcaaatgg gataaagcca
     1261 gcgattccca gcgcagcctg gctgaaaaat ggttgccctc aattcaggcc acagacgaaa
     1321 tgctgaacca ggggatttca acgaaaacgg cttttgcgac cgttgcaggg cattaccagg
     1381 tcagcgcatc cactttgcgg gacaagtatt accaggtaca gaagtttgcg aagcctgact
     1441 gggcggctgc acttgttgat ggacgtggag catcccgtcg caatgttcac aaaagtgaat
     1501 ttgacgagga tgcctggcag tttctgattg cagattatct gcgaccggaa aaacccgcct
     1561 tccgcaaatg ttatgagcgt ctggaactgg cagcccgcga gcatggctgg agtattccct
     1621 cccgtgccac ggcctttcgc cggattcagc aactggacga ggcaatggtt gttgcctgtc
     1681 gtgaaggtga acatgcactg atgcatctga taccggcaca gcagcg
//
LOCUS       DAJTYM010000234         1704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTYM010000234 DAJTYM010000000
VERSION     DAJTYM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..782
                     /locus_tag="OO575_004034"
     CDS             <1..782
                     /locus_tag="OO575_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR6666771.1"
                     /translation="AAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALILIAFLCV
                     DIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEAMIASL
                     EKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPYVHNVRR
                     VQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESLILGKR
                     PWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            949..1368
                     /gene="rbsD"
                     /locus_tag="OO575_004035"
     CDS             949..1368
                     /gene="rbsD"
                     /locus_tag="OO575_004035"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6666772.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            1376..>1704
                     /locus_tag="OO575_004036"
     CDS             1376..>1704
                     /locus_tag="OO575_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6666773.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNR"
BASE COUNT          410 a          419 c          439 g          436 t
ORIGIN      
        1 tggcggcggc gtacgggatt gcggtgaccg gaaccatggt actgacgtct attctctcga
       61 ctaccgtggc acgtcagaac tggcactgga ataagtattt tgttgcgctg atcctgattg
      121 ctttcctttg tgtcgatatt ccattgttca ccgctaacct cgataaactg ctctccggcg
      181 gctggttgcc attgagcctc ggtactgtga tgtttatcgt gatgaccacc tggaagagcg
      241 agcgtttccg cttgctgcgg cggatgcatg aacatggtaa ctctctggaa gcgatgattg
      301 cttcgctgga gaaatcaccg cctgttcgcg tgcccgggac cgcggtgtat atgtcgcgtg
      361 caatcaacgt cattcccttt gcgctgatgc ataaccttaa acataacaag gtattgcatg
      421 agcgggtgat tctgttaact ctgcgcaccg aagacgcgcc atatgtccat aacgtccgtc
      481 gggtacagat tgaacaactg tcgcccactt tctggcgcgt ggtggcaagt tatggttggc
      541 gagaaacgcc aaacgtggaa gaagttttcc accgctgcgg tctggaagga ttaagttgcc
      601 ggatgatgga aacctccttc tttatgtcgc atgagtcgtt gatcctcggc aaacgcccgt
      661 ggtatttgcg tctgcgcggc aagctgtact tgctgctgca acgtaatgcg ctgcgtgcac
      721 cagatcaatt tgaaatcccg ccaaacaggg ttatcgaact gggtactcag gtcgaaatct
      781 aacgccagac gcctcctttc ttcataaggg ggcgtttttg ttttcatggt taatcaccat
      841 gtaaaacgtt tcgaggttga tcacatttcc gtaacgtcac gatggttttc ccaactcagt
      901 caggattaag ctgcggatca gcgaaacgtt tcgctgatgg agaaaaaaat gaaaaaaggc
      961 accgttctta attctgatat ttcatcggtg atctcccgtc tgggacatac cgatacgctg
     1021 gtggtgtgtg atgctggttt acccatcccc aaaagtacaa cgcgtatcga tatggcatta
     1081 acccaaggtg taccttcttt tatgcaggtg ctgggcgtcg tcacaaatga aatgcaggtc
     1141 gaggctgcca ttatcgcgga agagatcaaa caacataatc cgcaactcca cgaaacgttg
     1201 ctcactcacc ttgagcagct gcaaaaacac cagggaaata ccattgaaat tcgttacacc
     1261 acgcatgaac aattcaaaca acaaaccgca gaaagtcagg cggtaattcg cagcggagaa
     1321 tgttctccgt atgcgaatat cattctctgt gctggcgtga cgttctgagg ccgtcatgga
     1381 agcattactt cagcttaaag gcatcgataa agccttcccg ggcgtaaaag ccctctcggg
     1441 cgcagcgtta aatgtctatc cgggccgtgt gatggcgctg gtgggcgaaa acggcgcggg
     1501 taaatccacc atgatgaaag tgcttactgg tatctatact cgcgatgccg gcacgctttt
     1561 atggctgggg aaagaaacga catttaccgg gccgaaatct tcccaggaag ccgggattgg
     1621 gattatccat caggaactga acctgatccc gcagttgacc attgccgaaa acattttcct
     1681 cggtcgtgag tttgttaatc gctt
//
LOCUS       DAJTYM010000235         1693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTYM010000235 DAJTYM010000000
VERSION     DAJTYM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(26..214)
                     /locus_tag="OO575_004037"
     CDS             complement(26..214)
                     /locus_tag="OO575_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6666774.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO575_004038"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO575_004038"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6666775.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
BASE COUNT          484 a          420 c          372 g          417 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctg
//
LOCUS       DAJTYM010000236         1630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTYM010000236 DAJTYM010000000
VERSION     DAJTYM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..391
                     /gene="bglJ"
                     /locus_tag="OO575_004039"
     CDS             <1..391
                     /gene="bglJ"
                     /locus_tag="OO575_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6666776.1"
                     /translation="IEARLIGSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDR
                     LNNQWYINQSRTLSPTEREILRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVS
                     SDAGLLEAEDILLCMRHCEASNVLHPY"
     gene            complement(429..1217)
                     /gene="fhuF"
                     /locus_tag="OO575_004040"
     CDS             complement(429..1217)
                     /gene="fhuF"
                     /locus_tag="OO575_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6666777.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            1358..1594
                     /locus_tag="OO575_004041"
     CDS             1358..1594
                     /locus_tag="OO575_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6666778.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          413 a          360 c          430 g          427 t
ORIGIN      
        1 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
       61 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
      121 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgt taagcccgac
      181 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
      241 gcttaaacgt aatatcaaaa cgatccgagc gcataaattt aatgtgatgt cgaaactggg
      301 cgtcagttct gatgcagggt tgttggaggc cgaagatatt ctgttatgta tgcggcattg
      361 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
      421 cagtgctttc atttcagcgt acaatagcca cattgctgca catccggtaa gcgataacgc
      481 tggcagcaag tgcggcgcac cagcaggccg tcgcgcagta ccacggtacg ccagagtgga
      541 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaaca
      601 gtagcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
      661 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcttg cacaaccgga
      721 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
      781 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
      841 tggaaatgtt ccggcgacac atctaatgct ttttcctgcg tcagtagcgc cagcattaat
      901 ggtggcacca tcaggccgat ataccattgt gcccatagtg agatcagcgg tttgttctcg
      961 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgccag tagagaactt
     1021 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1081 aggcggataa actccagcaa atgttcacga tgtttggcga tcatcgcccg tacggcttgt
     1141 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgacatcttc atagagcggt
     1201 gcggaacgat aggccataat cgtgatagta atctaaatga taatgattgc taatcctagc
     1261 gataggttta ctcgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     1321 gatattggtt ttcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     1381 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     1441 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     1501 tcacaaacag ttgcggcatt acattttgct accatcgtgc ctggcgctta ttgctggcat
     1561 catgctgatg ataatgaatt tgaatcaggg atgacaaaac agggaagaaa caagaaagga
     1621 agtaaagata
//
LOCUS       DAJTYM010000237         1616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTYM010000237 DAJTYM010000000
VERSION     DAJTYM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            6..224
                     /locus_tag="OO575_004042"
     CDS             6..224
                     /locus_tag="OO575_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6666779.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO575_004043"
     CDS             226..591
                     /locus_tag="OO575_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6666780.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO575_004044"
     CDS             588..1253
                     /locus_tag="OO575_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666781.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..>1616
                     /locus_tag="OO575_004045"
     CDS             1253..>1616
                     /locus_tag="OO575_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6666782.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          445 a          329 c          426 g          416 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaat
//
LOCUS       DAJTYM010000238         1594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTYM010000238 DAJTYM010000000
VERSION     DAJTYM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            240..509
                     /locus_tag="OO575_004046"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO575_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO575_004047"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO575_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO575_004048"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO575_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6666783.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          472 a          327 c          376 g          419 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agag
//
LOCUS       DAJTYM010000239         1593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTYM010000239 DAJTYM010000000
VERSION     DAJTYM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..159
                     /locus_tag="OO575_004049"
     CDS             <1..159
                     /locus_tag="OO575_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane;protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IIC forms the translocation channel for the sugar
                     uptake; involved in N-acetylgalactosamine transport;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR6666784.1"
                     /translation="FVCYIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNE
                     EEDYSNGI"
     gene            149..940
                     /gene="agaD"
                     /locus_tag="OO575_004050"
     CDS             149..940
                     /gene="agaD"
                     /locus_tag="OO575_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR6666785.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            941..>1593
                     /locus_tag="OO575_004051"
     CDS             941..>1593
                     /locus_tag="OO575_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR6666786.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEG"
BASE COUNT          391 a          383 c          421 g          398 t
ORIGIN      
        1 tttgtttgct acatccgggt cagcaacctg ttgccggttg ccgtactggg cgcaggcttt
       61 gcggtgtatg agtttttcaa tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc
      121 agtaaaaatg aagaagagga ctacagcaat gggatctgaa atcagtaaaa aagatatcac
      181 ccgtctgggc tttcgttcgt cgctgctgca agcgagcttt aactacgaaa ggatgcaggc
      241 gggcggtttt acctgggcga tgttgccgat cctgaaaaag atttataagg acgacaaacc
      301 gggcttaagc gcggcgatga aagataacct cgaatttatt aatacccacc cgaatctggt
      361 cggattcctg atggggttat taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat
      421 taaaggcctc aaagtggcac tgtttggccc aatcgccggg attggcgatg cgattttctg
      481 gtttactttg ttgccgatta tggcgggaat ttgctcatca tttgccagcc agggaaacct
      541 gctggggccg attctatttt tcgccgttta cctgcttatc tttttcctgc gcgtcggctg
      601 gacccacgtc ggttattcag tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca
      661 gatgattgct cgctcggcaa ccatcctcgg gatcacggta atcggcgggc tgatcgcttc
      721 gtatgtgcat attaacgtgg tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct
      781 gcaacaggat ttcttcgata aagtcttccc gaacatttta ccgatggcct acaccctgct
      841 gatgtattac ttcctgcggg tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt
      901 tgtgctctct attgtttgtt ccgcattcgg cattttgtaa atggaacgag gcactgcttc
      961 tggcggtgcc tctttactta aggaatttca tcctgtgcaa acccttcagc aagttgaaaa
     1021 ctatacggcg ttaagtgaac gagccagcga atatttattg gctgtgatcc gtagcaaacc
     1081 ggatgccgtg atttgcctgg cgaccggagc cacgccatta ctgacgtatc attatctggt
     1141 agaaaaaatc caccagcagc aggttgatat cagccagctc accttcgtga agctcgacga
     1201 atgggtggat ctgccattaa cgatgcctgg cacctgcgaa actttcctac aacagcatat
     1261 cgtgcagccg ctggggctac gtgaagacca gctcatcagc tttcgctccg aagagataaa
     1321 tgagacagag tgcgaacggg taacgaacct gattgcgcgc aaaggcggtc tggatttatg
     1381 cgttctcgga ttggggaaaa acggtcatct tgggctgaac gaaccgggag aaagcctaca
     1441 accggcctgc catatcagtc aacttgatgc cagaacacag caacatgaga tgttaaaaac
     1501 cacgggtcgc cccgtgactc gtgggatcac cttaggcctg aaggatattc tcaatgcccg
     1561 cgaggttttg ttattggtga ctggcgaagg aaa
//
LOCUS       DAJTYM010000240         1565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTYM010000240 DAJTYM010000000
VERSION     DAJTYM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="OO575_004052"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="OO575_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6666787.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
BASE COUNT          397 a          422 c          370 g          376 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgt
//
LOCUS       DAJTYM010000241         1532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTYM010000241 DAJTYM010000000
VERSION     DAJTYM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..291
                     /locus_tag="OO575_004053"
                     /pseudo
     CDS             1..291
                     /locus_tag="OO575_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            293..659
                     /locus_tag="OO575_004054"
                     /pseudo
     CDS             293..659
                     /locus_tag="OO575_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            587..1060
                     /locus_tag="OO575_004055"
                     /pseudo
     CDS             587..1060
                     /locus_tag="OO575_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          379 a          380 c          461 g          312 t
ORIGIN      
        1 tcaacgtatt accactgtca gcaacagcga catcatcatg ataaacgcag tgcccgtgcg
       61 cagcgcgatg actggctgaa gaaagagata ctgcgcgtat acgatgagaa tcatcaggta
      121 tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag gtatcagagt ggccagatgc
      181 actgtggcac gtctcatggc ggttatgggg cttgccggtg ttctccgggg taaaaaggtc
      241 cacactaccg tcagccggaa agccgttgcc gcaggcgacc gcgtaaaccg tcatcaaggc
      301 aatgtgccgc gtactccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac
      361 aaggataaac acacgtccgc agttccgtca gcactgcgac agcgttgtcc gcaaggcttt
      421 tacccagtcc agcgttacgg tgccccacgc ctgacggatg aactgtgtgc tctggttacc
      481 actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca ggaactgagc gcaaaagcct
      541 cccagaagtt cagcccgatc agttaccgtg cacacggcct gcctgtatca gaaaatctgc
      601 tgacgcagga tttttacgcc agtggcccga accagaagtg ggcgggagac atcacgtact
      661 attacagctc accgactgcg gggaaacacg gggcgccggg ttattgaagg tgacctgtcc
      721 agctacttcg acacagtaca tcaccggctg ctgatgaaag ccgtctgccg caggatcagt
      781 gatacacgtt ttatgagact gctgtggaaa acgccgtgtt gatgttggtc tcttccgggc
      841 ggtcagtgaa agtatgccac agggcggtgt gatctcgccg ttgctgtcga acatcatact
      901 gaatgagttc gaccagtatc tgaataaacg ctacctgagt ggaaaagcca gaaaggatcg
      961 ctggtactgg aatcacagta tccagcgagg ccgaagtact gctgtcaaag aaaactggca
     1021 atggaaacct gccgttgctt actgttgcta tgccgatgac tgtgtgccac gaaggcgcgt
     1081 cctggggacg tgagtccctg tgcagccatg cagcacaaga gatgagggaa ggccccttgg
     1141 tgtcggcgtg caggcgcagg caccaaacca tccactgctg ctgcatttaa gagatgtggt
     1201 ggtgagcgag atgggaaaag gtcggacgtg agccgatcag gtatggttcg aagagacgaa
     1261 cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat gatgtcaaaa caatggtgga
     1321 gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc ggcataaaag cggcgtgaag
     1381 tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc gatgtgaagg gagaagcgca
     1441 agtggaagcc ccatgagcgc cagagtacca atgcggggta cagggacgga gcaccaagcg
     1501 attgcgcagt aaacatgctt gacaacttaa tt
//
LOCUS       DAJTYM010000242         1489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTYM010000242 DAJTYM010000000
VERSION     DAJTYM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..754
                     /locus_tag="OO575_004056"
     CDS             <1..754
                     /locus_tag="OO575_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="regulates the transcription of lsr genes; involved
                     in autoinducer 2 uptake, biofilm formation and stress
                     responses; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6666788.1"
                     /translation="FEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRLGIGAAHMLM
                     SLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGIGQLNAACSV
                     NIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDDATIIRSGYI
                     SQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTIPVRVGVAGG
                     ENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            833..>1489
                     /locus_tag="OO575_004057"
     CDS             833..>1489
                     /locus_tag="OO575_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6666789.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     S"
BASE COUNT          371 a          352 c          410 g          356 t
ORIGIN      
        1 ctttgaaggc tgtctggaat atgaaactca attacgtcgt cagttttcgc tgcaacatgt
       61 ccgggtgatc cctgggcttg cggatgctga tgtcggcggg cgactaggga taggcgcggc
      121 gcatatgttg atgagtttac ttcaaccaca acagatgctg gcgattggtt ttggcgaggc
      181 aaccatgaat acgctgcaac gcttaagtgg ttttatttcg tcacagcaaa ttcgcctggt
      241 cacgctctcc ggtggcgtcg gttcttatat gacgggtatc gggcagctta acgcggcgtg
      301 cagtgtgaat attattccgg ctccgttgcg ggcatcctcc gctgacattg cccgtacgct
      361 aaaaaatgaa aattgcgtca aagatgttct gctagccgcg caagcagcgg atgtggcgat
      421 tgtcggcatt ggagccgtga gtcaacaaga cgacgcgaca atcattcgct ccggttatat
      481 cagccagggc gaacagttaa tgattggccg aaaaggggcg gttggcgaca ttttaggcta
      541 cttttttgat gcaaaaggtg acgttgtcac ggatatcaaa atacataacg aactgattgg
      601 cttaccttta agcgcgctga agaccatacc cgtccgggtt ggcgtggcag ggggagaaaa
      661 taaagctgaa gcaattgccg ctgcaatgaa aggcggttat atcaacgcac tggttaccga
      721 tcaggacaca gcagcggcga ttttacgtag ttaaatttgc atgaccttat taccgggaat
      781 gatggagaac gtatagagga acagacagaa cgcataagcc gaggataatc taatggctcg
      841 actctttacc ccttcagaat caaagtacta cctgatggcg ctggatgcag gcaccggaag
      901 tattcgggct gtgatattcg acctggaagg caatcaaata gcagtgggac aggcggagtg
      961 gcggcatctg gcagtaccgg acgttcctgg ttctatggaa tttgatctca acaaaaactg
     1021 gcaactggcg tgtgagtgta tgcgccaggc gctgcacaac gccggcatag acccggagta
     1081 tatcgctgcc gtttcggcat gttctatgcg tgaaggcatt gttttatata acaatgaagg
     1141 aacaccgatc tgggcctgcg ccaatgtgga tgccagagcg gcacgcgaag ttagcgaact
     1201 taaagaactg cacaacaata cctttgaaaa cgaagtttat cgagcgaccg gacaaacact
     1261 ggctttaagt gccatcccca gattactttg gctggcgcac catcgttccg atatttaccg
     1321 tcaggcatca accatcacca tgatcagcga ctggctggcc tatatgctca gcggcgaact
     1381 ggcggtggat ccctctaacg ctggcaccac gggacttctt gatctaacca cccgtgcctg
     1441 gaaacctgca ttgctggata tggctggcct gcgtgccgat attctttct
//
LOCUS       DAJTYM010000243         1486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTYM010000243 DAJTYM010000000
VERSION     DAJTYM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO575_004058"
     CDS             complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO575_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6666790.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1117..>1486)
                     /gene="sitC"
                     /locus_tag="OO575_004059"
     CDS             complement(1117..>1486)
                     /gene="sitC"
                     /locus_tag="OO575_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6666791.1"
                     /translation="HARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPG
                     ATAWLLTDRFPRLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFA
                     PTHGLLANRRRAHKALEDRS"
BASE COUNT          422 a          368 c          374 g          322 t
ORIGIN      
        1 tgaaggaaga gctgaatagt ctgaaggatt tgaattcggt taggaatttt atcgtggaat
       61 aacagaagct gcggcacgtc gtatgcagga acgtgccgcg gttggctggt aaactttcga
      121 tagtgcgagt attgaatgat ttctaggcgt tatcgatttt acgtattttt tgcatgatag
      181 aattcgtacc tcctcccaac gaccttccat ggctttccgg acttctgtag gctggcggac
      241 gatgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggcgtttc
      301 cctttgtgcg tgcgttgatg ctggtaacgg taaacgtcat gatgaacacg accgcgaata
      361 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacagccag acaccagtca
      421 ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag cgttgtgtca
      481 ggagtactgc aatggcgcca ggcgcaatga gcagagaaat agagagaatg atacctactg
      541 ctttcagcgt cgccacaatg gtcagggaaa ccatgcacag cagcccataa tgcagccatc
      601 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
      661 gccattttac gctaataaca agtgtaacca gcccggcaat aatcaccgtc tggattatgt
      721 cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt
      781 taattgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccataa
      841 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
      901 ctccggcgac gaatgcccct gtagccagag gtaaccccag aatccaggta agcacgatgc
      961 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
     1021 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
     1081 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt
     1141 atgcgcacgc cgacgatttg ccaaaagtcc atgggtgggc gcaaatacaa atgctaaaag
     1201 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
     1261 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatta tgagcaggcg
     1321 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
     1381 cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
     1441 gaggattttt aatctgcccg gatgtaatcc aatagcacgg gcgtga
//
LOCUS       DAJTYM010000244         1475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTYM010000244 DAJTYM010000000
VERSION     DAJTYM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            201..452
                     /locus_tag="OO575_004060"
     CDS             201..452
                     /locus_tag="OO575_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666792.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            519..797
                     /locus_tag="OO575_004061"
                     /pseudo
     CDS             519..797
                     /locus_tag="OO575_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            799..>1475
                     /locus_tag="OO575_004062"
     CDS             799..>1475
                     /locus_tag="OO575_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6666793.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          361 a          375 c          385 g          354 t
ORIGIN      
        1 aaatccctcg ccacctctga tgtggcaggc atcctcaacg cacccgcact taacccgctt
       61 cggcggtttt ttgtttttat tttcaacgca tttgaagttc tggatggtgc cggaatagaa
      121 tcaaaaatac ttaagtagcg cgcagggata aaagggatgg accccgaaca ggggagtgct
      181 acttatctgg aaggattctg atgatgaaca tcgaagaact gcgtaaaatt ttttgtgaag
      241 atggcctcta tgctgtgtgc gttgaaaatg gaaatattgt tagtcattac cgcattgtgt
      301 gtttgcaaaa aaatggggct gcgttaatta attttgtgga tgcccgagtg acggacggat
      361 ttatcttgcg cgacggtgag tttgtcactt cattacaggc attgaaagag atcggaataa
      421 aagctggctt ttctgctttt tcagaagaat aaactcatct acaatcttgc gcggggctga
      481 actcccgctg agtaacaccg tgccaccgga gaaaaccgat ggcacgcaac gcaaaatatt
      541 acaatcatga taattcgacc gttcttgccc acacgcacga gcggtattct cacgcattta
      601 agtcagactg gtaccagcat ccctcatgca ctgaagaaca ggccgaatgg cttattcagt
      661 gttaccgcag gcgcggatgc gaggttaaaa aagcccttag cctcgactac cgtcactaga
      721 taatctccgt caggctccct tactccgaac agccaccgcg tccgtcccgc acattccagc
      781 aacggatctg gaggtaacgt gcgggtatta cttcgacctg ttctggttcc ggaactcggg
      841 ctggtggtcc ttaagcccgg tcgtgaatcc atgccggtat tccacaatac ccgggtactg
      901 gtggagccgg aaccgaaaag catgcgtaat ctgccgtccg gggtcgttcc tgccgttcgc
      961 cagccgctag tggaagacaa aacattgctg ccgtttttca gtaacgcacg ggtgattcgt
     1021 gctgctggtg gtgctggcgc attgtctgac tggctgttgc gccatattaa atcctgccag
     1081 tggccacacg gcgattatca tcacagcgaa accgtcattc accgttatgg taccggcgca
     1141 atggtattgt gctggcactg cgacaaccag ctgcgtgacc agacatccga atcactcgag
     1201 caacttgctc atcaaaacct gtcagcatgg atgattgacg tcatcggtca cgcaataagc
     1261 ggtacgcagg agcgtgaatt atctctggct gaattatcct ggtgggcggt ccgcaatcag
     1321 gtggcggacg cgctaccgga agcggtatta cgtcgttcgc tggggttgcg tgcggaaaaa
     1381 atccgctcaa tgtaccgtga aagcgacatc gtgccgggag agcagaccgc caccagcata
     1441 ctgaagcagc gcacaaaaaa tcttgcgccg ctgcc
//
LOCUS       DAJTYM010000245         1474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTYM010000245 DAJTYM010000000
VERSION     DAJTYM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(5..184)
                     /locus_tag="OO575_004063"
                     /pseudo
     CDS             complement(5..184)
                     /locus_tag="OO575_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            708..992
                     /locus_tag="OO575_004064"
     CDS             708..992
                     /locus_tag="OO575_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6666794.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            992..1267
                     /locus_tag="OO575_004065"
     CDS             992..1267
                     /locus_tag="OO575_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6666795.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          402 a          369 c          361 g          342 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga catt
//
LOCUS       DAJTYM010000246         1433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTYM010000246 DAJTYM010000000
VERSION     DAJTYM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            82..1275
                     /locus_tag="OO575_004066"
     CDS             82..1275
                     /locus_tag="OO575_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6666796.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1433)
                     /locus_tag="OO575_004067"
                     /pseudo
     CDS             complement(1295..1433)
                     /locus_tag="OO575_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          421 a          309 c          336 g          367 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aac
//
LOCUS       DAJTYM010000247         1427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTYM010000247 DAJTYM010000000
VERSION     DAJTYM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..784
                     /locus_tag="OO575_004068"
     CDS             <1..784
                     /locus_tag="OO575_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6666797.1"
                     /translation="IFIIVGFKSGWRMQVSTEELFVSIMKWLPFTALGMPNINDVKDS
                     FTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISLFVFILFFSKIHIAS
                     FLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTYAPLPLILCGITFII
                     IASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPNNYTENTFIILVSTT
                     AFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(838..>1427)
                     /locus_tag="OO575_004069"
     CDS             complement(838..>1427)
                     /locus_tag="OO575_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6666798.1"
                     /translation="GGADLAIDNTGTRPVTVVINGGNFHRVSSARYTHTNIQVTSSGG
                     GKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETTSKVTSASALSVT
                     RSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSC
                     DVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          444 a          302 c          250 g          431 t
ORIGIN      
        1 catttttatc attgtaggtt ttaagtctgg atggagaatg caggtatcca cagaagagct
       61 ttttgtgtca ataatgaagt ggttgccatt cactgcgcta ggtatgccga acattaatga
      121 cgtaaaagat tcatttacta tcaatgccgc tgtaacatgg acacttgtat atgaatggtt
      181 cttttatttt tctcttccgg taatttccgc gctcataaaa agaaaagtca gtatttacat
      241 ggttatgatt agcgcaatat cgctatttgt tttcatttta tttttcagta aaatacacat
      301 agcatcattt ctttttgggc tcctggcatt tttgctaaat aaatcaaaga tagtcaatgg
      361 aattgccaaa gcaaaagtaa caccaattat aataactgcg ataatggttt ttgaaatgac
      421 gtatttcaaa acaacttacg caccgctacc actgattctt tgtggaataa catttatcat
      481 tatcgcatca ggttgtgatt tgtatggaat attaagatta aatataacca gaaagctagg
      541 agaaactacc tacagcgttt accttctgca tgggatattc ctttattgcc taatgacgtg
      601 gattattcct aataattaca ctgaaaatac atttatcata ctggtaagca caactgcatt
      661 tcttattacg ttcacatcat gcctaacttt taaattaata gagacgccat tcatcaaatt
      721 aacaaaacaa accacaactt tagtaaaaga attaataccc acattaacaa acaacaatca
      781 ataaacaata aacataagcc gccctacata tatgtccaaa tggggcggcg aatgtattta
      841 tattagtctg gtaatgctaa atgcaaacga actggagatt cctgccccgc taagcgtatt
      901 gcgcagggtc accctaaatg tatctacgcc aatataagta acatcacaac taactgaatc
      961 aggcgcagat ataggtgtta tctgcacaat ataaccgcta gcaactgctg cgagcggatg
     1021 agaaaagcta acatcataca cacctgtaga aaccacgtta accgatgaaa caccagtagc
     1081 aacgtcgata ctaccattgg cgttaatttt tcccgagcgt gtgactgaca atgcagaagc
     1141 cgatgttacc ttactggttg tttctgtaaa aacacaccca atgtcaacta cctcgcaatt
     1201 agctcctgtt atccagtatg gtcgttctgc acttggctga taatcccctg cgctctggaa
     1261 cgttgtgccg ttgagtatga ctgtaacttt tcccccacct gagcttgtta cttgaatatt
     1321 cgtgtgcgtg tatcttgcac tgctcacgcg atgaaaattt ccaccgttta taaccacagt
     1381 tacagggcgc gtcccagtat tatctatagc aaggtctgcc cccccgg
//
LOCUS       DAJTYM010000248         1412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTYM010000248 DAJTYM010000000
VERSION     DAJTYM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            367..>1412
                     /locus_tag="OO575_004070"
     CDS             367..>1412
                     /locus_tag="OO575_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6666799.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          459 a          306 c          226 g          421 t
ORIGIN      
        1 gtacattgag atatgatttt gatcattact aaatcctttg ctgaagttac tatgtatata
       61 agcttatttt atacatgatt attagcaaca aatattacca agtcaagtag tcaggctggc
      121 taaaatagtt ggagatgttg tagtatctta ctcaagacta atgtcctctg gtataaatcc
      181 tctgcattgt gaatatgtaa aacatcaaaa aaagatgaac caaatatacg aaaaaagcaa
      241 atatacttac tagggtagaa agcatgatat aacactcatt cccttatggc gaatattctg
      301 aaacaccctc ctcattagga gagatacatt aaatacaatt tattcacaat ctaattccat
      361 atccatatgc gagaaattaa tatgctcaga aacatttcat cctgtttatt tccacatatc
      421 agcacaatta catcccccaa ccattatttg tccgaatggg atgattggga gaaacagggg
      481 ttaccggaag aacagcgtac tgaggcggta agaagacttc gtgcatgtct tacctctaag
      541 gggcataaac tggacctgcg agccttggcg ctttcctcgc tacctgtact ccctgcttgc
      601 attaaaaagc ttgatgtgag ctgtaataaa ttaaccatcc ttactgattt acctgaaaat
      661 attaaagaac ttattgcaag agataatttc ttaacacata tatctgcatt accacattat
      721 ctaataactt tggatgtgtc cgaaaatcaa ttagagaatc tgccgttatt accagacacc
      781 atcaaatcac taagcgcaga gtataatagg ttatccacac tgccttcatt acccttgaat
      841 ttaaaaaaac ttgaggttag gaacaacgaa ctgcaaactc ttccatctct gccttctaat
      901 cttaagatac ttaaggttgc gcacaaccat cttactgaac tgcccccttt acctaggaga
      961 ctgcaacttc tttttgcata tagcaataga ttaagcaact taccaaacat ccaagaaaat
     1021 attatcatga gaagattttt ttattttgaa aacaaccaaa taactacaat cccgacaaat
     1081 ctttttcgtt tagatcctca tataactatt gagattgcaa ataacccctt atcagatcaa
     1141 actctgctat tcttaataca gcaaacttcg gttccaaatt ttaacgggcc tcagtttcgt
     1201 atttccctgt cagaccaaaa cagactgttt ttacgccaga tgttgccgca aaatttacat
     1261 tcgcgccata tcagagtcat cactgaaggg gggcagaact ttcagatccc ccctcttccc
     1321 gaaactgtgg cagcctggtt tcctgaagca gatcgtcggg aggtttctac acaatggact
     1381 tctttttcca ccgaggagaa ttcccgggca tt
//
LOCUS       DAJTYM010000249         1406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTYM010000249 DAJTYM010000000
VERSION     DAJTYM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..169
                     /locus_tag="OO575_004071"
     CDS             <1..169
                     /locus_tag="OO575_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6666800.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            154..792
                     /locus_tag="OO575_004072"
     CDS             154..792
                     /locus_tag="OO575_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6666801.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..>1406
                     /locus_tag="OO575_004073"
     CDS             1020..>1406
                     /locus_tag="OO575_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666802.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          348 g          394 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttc tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg actctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tgcaggaaaa cgcctgaggg gctggttgcc tgtagcctgc
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata agatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 cgaaaatatt taaatacatt gaaatccctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagt
//
LOCUS       DAJTYM010000250         1402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTYM010000250 DAJTYM010000000
VERSION     DAJTYM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..729
                     /gene="ccp"
                     /locus_tag="OO575_004074"
     CDS             <1..729
                     /gene="ccp"
                     /locus_tag="OO575_004074"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6666803.1"
                     /translation="SIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLN
                     PIEMASKSWDEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFD
                     KWLRGDENALTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAAD
                     IGRMNVTKEERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQE
                     DVDDIVAFLHSLNGVYTPYMQDKQ"
     gene            940..>1402
                     /locus_tag="OO575_004075"
     CDS             940..>1402
                     /locus_tag="OO575_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6666804.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDK"
BASE COUNT          387 a          278 c          357 g          380 t
ORIGIN      
        1 tcgattggtg ttggtggcgc agttgggccg attaatgcgc cgacggtatt taactcagta
       61 tttaacgttg agcagttctg ggatggtcgt gcggcaacat tgcaggatca ggcaggtgga
      121 ccgccgttga acccgattga aatggcgtcg aaatcctggg acgaaattat tgctaagctg
      181 gaaaaagatc cgcagcttaa agcgcagttc ctcgaagttt atccgcaagg tttcagtggc
      241 gaaaatatta ctgatgccat tgctgaattt gagaaaacat taattacgcc ggattcccca
      301 tttgataaat ggttgcgtgg agatgaaaat gcgctgacgg cgcaacagaa aaaaggctat
      361 caattattta aagataataa atgtgcaact tgtcatggtg gtattattct cggcggacgt
      421 tcctttgaac cgttggggct gaaaaaagac tttaactttg gggaaattac ggcggcggat
      481 attggtcgta tgaatgtgac taaagaagag cgtgataaat tgcgtcagaa agtacccggt
      541 ttacgtaacg ttgctttaac ggcaccgtac ttccatcgcg gtgacgtgcc gacgctggac
      601 ggggcggtga aactgatgct gcgctatcag gtaggcaaag agctgccgca ggaggatgtg
      661 gatgatatcg tagctttcct gcacagtctg aacggggtgt acacgccgta tatgcaggat
      721 aaacaataat taatttgatc gcccgaacag gaatgtttgg gcgattttta ttacgataat
      781 aaagtctgtt tttaatatta tcatgttaaa tgtttatatt ataaaaagtc gtttttctgc
      841 ttaggatttt gttatttaaa ttaagcctgt aatgccttgc ttccattgcg gataaatcct
      901 acttttttat tgccttcaaa taaatttaag gagttcgaaa tggaccagaa gctgttaacg
      961 gatttccgct cagaactact cgattcacgt tttggcgcaa aggccatttc tactatcgcg
     1021 gagtcaaaac gatttccgct gcacgaaatg cgcgatgatg tcgcattcca gattatcaat
     1081 gatgaattat atcttgatgg caacgctcgt cagaacctag ccactttctg ccagacctgg
     1141 gacgacgaaa acgtccataa attgatggat ttgtcgatca ataaaaactg gatcgacaaa
     1201 gaagaatatc cgcaatccgc agccatcgac ctgcgttgcg taaatatggt tgccgatctg
     1261 tggcatgcgc ctgcgccgaa aaatggtcag gccgttggca ccaacaccat tggttcttcc
     1321 gaggcctgta tgctcggcgg gatggcgatg aaatggcgtt ggcgcaagcg tatggaagct
     1381 gcaggcaaac caacggataa ac
//
LOCUS       DAJTYM010000251         1383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTYM010000251 DAJTYM010000000
VERSION     DAJTYM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..1017)
                     /locus_tag="OO575_004076"
                     /pseudo
     CDS             complement(1..1017)
                     /locus_tag="OO575_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          321 a          362 c          327 g          373 t
ORIGIN      
        1 cccgtggata cattccacct tcagcgaatg aaagaagctt tccacacagg cattgtcata
       61 acagtagcct ttcgcactca tactgccacg caggttgtgt cgcttcagca ttgcctgata
      121 atcccctgaa cagtattgac caccacggtc cgtatgaaca atgacgcttt ccgggcgtct
      181 tctccgccac aacgccattt gcagtgcatc acaggccagt tgtgctgtca ttcgcggtga
      241 catcgaccag ccaatgacgg cgcgtgacca caggtcgatg actactgcga gatacaacca
      301 gccctcatcg gtacgcaagt acgtgatgtc acccgcccac ttctggtttc gggccactgg
      361 cgctgaagtc ctgctccagc agattctcca atacgggcag gccatgtgca cggtagctga
      421 ccgggctgaa cttccggctg gctttcgccc gcagcccctg acgacgcagg ctggcggcaa
      481 tggttttaat attgaactcc ggcagttcgt cagcaaggcg gggagcaccg tatcgctgct
      541 ttgcctcaaa gaatgcctta tggacagcgg catcgcaggt gagccgaaac tgttggcgca
      601 ggctcatctg gtgacgacgc ctgagccaga cataccagcc gctgcgggca acccgaagta
      661 cacgacacat cgctttgatg ctgaactctg cccgatgatt ttcgataaag acatacttca
      721 tttcaggcgc ttcgcgaagt atgtcgcggc cttttggagg atggacagtt cctcagcctg
      781 ctccgccagt tgtcgtttaa ggcggacatt ttcagcggcc agttcgcttt cgcgctctga
      841 cgaactcatt tgttgctgct gtttactgcg ccaggcataa agctgagatt catacaggct
      901 gagttcacgg gctgcggcgg ccacaccgat gcgttcagcg agtttcaggg cttcgttacg
      961 aaattcaggc gtatgttgtt tacggggctt cttgctgatt gatactggtt ttgtcatgag
     1021 tcacctctgg ttgagagttt actcacttag tcctgtgtcc actattggtg ggtaagatca
     1081 ccctccatta aaactaaaac gggcgctttc tgatatatca tctgtaagcg cagtaatacc
     1141 attagtagca cctcgcgcat tgattattga atattataac tggaatgtta tatttcttgt
     1201 tatgatgctg ttcagtatta taataatgtt attatcacca aagataactc atgcaaatga
     1261 gaaagtaata tgtcaaaaca gtgcatcctt tgtgaccaaa gagtttatct gcggcactat
     1321 acttgtctca ttaatactca gttctctatt ttgctacata tcaatatcac catcaatttt
     1381 cat
//
LOCUS       DAJTYM010000252         1370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTYM010000252 DAJTYM010000000
VERSION     DAJTYM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO575_004077"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO575_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6666805.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO575_004078"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO575_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..>1370)
                     /locus_tag="OO575_004079"
     CDS             complement(1292..>1370)
                     /locus_tag="OO575_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6666806.1"
                     /translation="KTICFSRSVEIHEKVIGSFIEKHIF"
BASE COUNT          439 a          205 c          286 g          440 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta
//
LOCUS       DAJTYM010000253         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTYM010000253 DAJTYM010000000
VERSION     DAJTYM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            400..1149
                     /locus_tag="OO575_004080"
     CDS             400..1149
                     /locus_tag="OO575_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6666807.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          387 a          278 c          275 g          426 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcac
//
LOCUS       DAJTYM010000254         1361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTYM010000254 DAJTYM010000000
VERSION     DAJTYM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..519)
                     /locus_tag="OO575_004081"
     CDS             complement(<1..519)
                     /locus_tag="OO575_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR6666808.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPE"
     gene            complement(470..1234)
                     /locus_tag="OO575_004082"
     CDS             complement(470..1234)
                     /locus_tag="OO575_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666809.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
BASE COUNT          312 a          365 c          305 g          379 t
ORIGIN      
        1 ttcgggcgga tcaacaacga agttgcgcca gacgaaatca gtaacaagtc cggggttgaa
       61 tatgaaccag cattccgaac cctctttacg gatcgttggc tccagaattt tccactggta
      121 ttccgtcagt gcgtgggctt cttccaacca caacatatcg atcccctcca gtgacttaat
      181 ttcttcgatg ttgcgccata atccataaaa aacaaattcc gagccagtaa cccggttaat
      241 gattttgttg ttcagaatac ggaaacggtg ccgcagacca aacctgtcta tctgaatttt
      301 gagcagggta tacaccgact cttcgatttt attctggatc tgacgggcac aacaaaaacg
      361 cagggtgtat ttattcgaca gaaatatggc aatgccagcg gcatcccatg attttgacga
      421 tgaccgacca ccataaagca ctttgttacg tgcccgcata gtccagaaac tacgtaaagc
      481 cggattcagc gtcggtttgg atgtcagagt agaagtcatt gaggtcacgc tctccattgc
      541 catcatcaat acctgcatca cggcgaagac gatcagcctc cagagacacc ttatcagtgg
      601 cggccttgcg gtaggctgta tcagcaaata tttttcctac cgtcgcaagc gtgccaacga
      661 tggactcaat acgaactgta ttgcgcatca ttgctttctc ggcggcgctg atattttcaa
      721 tcagcatctt cctttcctgg tccccattag catcttccag cgacaccaac caccgaccaa
      781 tattctctgc agcgacaagg ttgttagcac gaaggcgaaa taattcgtcc tcgagcgtca
      841 acgctttagc gtcctctatc acctcatctt tgagcagaag gcgacgggca taaccaccgt
      901 gttttaatgc ctgctggtta ccgggttgga atgggttagt cgggggatcg gtacgcattc
      961 cgcgtatcgg tttcgtatcc ggtgtagatt ctgtttttag ttgcgtactt ttttgtgtaa
     1021 ggccagtaat ggcaggcttt ctgctggtac gcactttcct tttttgcgta ccatttttgc
     1081 aaacctgcgt accgccactg cgtgcccaat caagcttttt ggccctcttc ctgatagccc
     1141 cttctgtaac gccgtatttc tcgcctatat cacggaggct aaggactccg gcccggtatg
     1201 ccgattcgat ggcctcccag tccggttttg ccatgattat gcttcctgtg atttactgtt
     1261 atcgctgcct gtcacaatag gttgctttaa ttcttcctca tcactcactc ctgcgacgct
     1321 ccgtggcagg gccccccaca ggagtacctt gtaaatgtaa g
//
LOCUS       DAJTYM010000255         1296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTYM010000255 DAJTYM010000000
VERSION     DAJTYM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            448..>1296
                     /gene="ipaH1.4"
                     /locus_tag="OO575_004083"
     CDS             448..>1296
                     /gene="ipaH1.4"
                     /locus_tag="OO575_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HCR6666810.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSA
                     TISAPEH"
BASE COUNT          450 a          261 c          216 g          369 t
ORIGIN      
        1 aaagataaat gaagccggaa ttgatgaaaa acaaagccaa cggtattctg atttctgaag
       61 ttaatttcag actcgtttaa gctccatttt caaaaaaaat atatcagggt ttgttactaa
      121 taacttctga aaacagaaaa aaatagagca gtaattctat ttcagagagg agtaactcca
      181 tctatcctcg atacgtactg aacgttatcg tgatatattc atcatgcttt cttcaccatt
      241 attcgagtat agggagagac gcaatgacct taaatacctt tttatggctg aattaccatt
      301 ctcaggaata acagcagact ccttcccata gacggcttag ggggaaagca atacccggag
      361 aaagggtaca aagcaaactg ttttaaaatt aataagccat attctattta actagaattc
      421 ttatcgttga taagaattct agttataatg attaaatcaa ccaatataca ggcaatcggt
      481 tctggcatta tgcatcaaat aaacaatgta tactcgttaa ctccattatc tttacctatg
      541 gaactgactc catcttgtaa tgaattttat ttaaaaacct ggagcgaatg ggaaaagaac
      601 ggtaccccag gcgagcaacg caatatcgcc ttcaataggc tgaaaatatg tttacaaaat
      661 caagaggcag aattaaattt atctgagtta gatttaaaaa cattaccaga tttaccgcct
      721 cagataacaa cactggaaat aagaaaaaac ctattaacac atctccctga tttaccacca
      781 atgcttaagg taatacatgc tcaatttaat caactggaaa gcttacctgc cttacccgag
      841 acgttagaag agcttaatgc gggtgataac aagataaaag aattaccatt tcttcctgaa
      901 aatctaactc atttacgggt tcataataac cgattgcata ttctgccact attgccaccg
      961 gaactaaaat tactggtagt ttctggaaac agattagaca gcattccccc ctttccagat
     1021 aagcttgaag ggctggctct ggctaataat tttatagaac aactaccgga attacctttt
     1081 agtatgaaca gggctgtgct aatgaataat aatctgacaa cacttccgga aagtgtcctg
     1141 agattagctc agaatgcctt cgtaaatgtt gcaggtaatc cattgtctgg ccataccatg
     1201 cgtacactac aacaaataac caccggacca gattattctg gtcctcaaat atttttctct
     1261 atgggaaatt ctgccacaat ttccgctcca gaacac
//
LOCUS       DAJTYM010000256         1291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTYM010000256 DAJTYM010000000
VERSION     DAJTYM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..412)
                     /locus_tag="OO575_004084"
                     /pseudo
     CDS             complement(1..412)
                     /locus_tag="OO575_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(463..1038)
                     /locus_tag="OO575_004085"
                     /pseudo
     CDS             complement(463..1038)
                     /locus_tag="OO575_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1042..1291
                     /locus_tag="OO575_004086"
                     /pseudo
     CDS             1042..1291
                     /locus_tag="OO575_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          288 a          370 c          323 g          310 t
ORIGIN      
        1 catgggccat acacgcagct tccgttattc tgccggattc gtataacgcc cggtaaccac
       61 cgtaagcatc tgcctgaagc acaccgctgt aaccggctag gtgattctgt ggatggatac
      121 ctttccggtc cgggctgtac gcgaaccaga ccgccggggg catctgtgaa ccggcgttac
      181 ggtcatcacg gacatagacc cacagccggg cagttcttgt tttaccactg cccggctcct
      241 ggaccggaac ggggatatca tccgcatgaa ctttaccggg catcagtaca tactggcgca
      301 ggacgtcata cagcggctcc agcagttcag caacggcacc ggtccagcga cccagtgtgg
      361 cacggctcag ctccacgccc tgacgacggt atatttctga ctggcggtat aactgtcaac
      421 gacggatgaa aagtggctct tccgcaacct gggtggagat tttcatgcca gtggactcat
      481 taccctctgc ctgagtagtt caaatcctgc tcgaccatac atctggcgtt tcagcatctt
      541 cttcaggcga tttacatgac cttcgacaac accattactc cagcgactac tgattgcctc
      601 acatatagct gcagcatcag cttccatccc cgccgccacg cgccgaaatt ctgctgaacc
      661 gctttcgtgg actcgagtga accaactgct tagctgtgat ttattttggg ttttcagaat
      721 acggtagaac tcgagtgcca gttgctgcgc tattttcagc tcaggttctt tttcacacat
      781 cagactaata aatcgggaag cataattttc ttcatccctg gttattctcc agggcatcaa
      841 ccagcggctt acccgggaga ctgaaggaag tctgacggca gtagtaaccg gtgggttcca
      901 gcctttacgc catttggcaa ccgcatccct gacgatggtt tcactgcctg taaatccccg
      961 ggccaccatt tcccgccata tccggctggc gttataatta ccactttctc gttgctcttt
     1021 taaccactcc ctccatggtg tcagcgaaaa acgaaaccgc tgttgaaatc cctggaaagc
     1081 tggttgcgga aaagatgaag acccagtcgc gacactcaga gttggcgaag gcgttcgcgt
     1141 acgcacttaa ccagtggccg gcactgacgt actatgcgaa cgatggctgg gtggaaatcg
     1201 acaacaacat cgctgaaaat gccttgcggg cggtcagtct gggtcgtaaa aacttcctgt
     1261 tcttcggctc tgaccatggt ggtgagcggg g
//
LOCUS       DAJTYM010000257         1283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTYM010000257 DAJTYM010000000
VERSION     DAJTYM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..153
                     /locus_tag="OO575_004087"
     CDS             <1..153
                     /locus_tag="OO575_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6666811.1"
                     /translation="QNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQLQLDERT
                     EPDAIG"
     gene            150..599
                     /locus_tag="OO575_004088"
     CDS             150..599
                     /locus_tag="OO575_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6666812.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(600..833)
                     /locus_tag="OO575_004089"
     CDS             complement(600..833)
                     /locus_tag="OO575_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6666813.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(919..1092)
                     /gene="ortT"
                     /locus_tag="OO575_004090"
     CDS             complement(919..1092)
                     /gene="ortT"
                     /locus_tag="OO575_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6666814.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
BASE COUNT          306 a          308 c          323 g          346 t
ORIGIN      
        1 caaaatcagg cttcgctgca tctgcaccag tcgctgggat ttgtcgtcac cgcacaaatg
       61 ccgcaggtag gcactaaatt tggtcattgg ctggatctga catttatgca gttgcaactc
      121 gacgagcgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
      181 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggcgcgcatc acccagacct
      241 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
      301 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
      361 ggtggacact tatacctggc ttgctgggtt cgttttttgt cttcgccagt atcagcggat
      421 atcagaatgt gggagcggcg acaactattg cggtactggt cgccagccag ctgattggcg
      481 gtctggtgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
      541 tatgtggcgc aattttactt gtggttggcg cctggttggt ggccagacgc tcattttaat
      601 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
      661 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
      721 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
      781 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
      841 ccttagccac ttgtgaaatt tatcgattaa tccaaatgat ataagtctgc gtggtgccgt
      901 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
      961 ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     1021 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     1081 atagagaaac atgtacgggc gtccagccat atgaataaaa cgcgcgcaaa tatgcgccat
     1141 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     1201 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     1261 aggggcaatg ataaaaggtg gca
//
LOCUS       DAJTYM010000258         1283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTYM010000258 DAJTYM010000000
VERSION     DAJTYM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            129..1127
                     /gene="yiaK"
                     /locus_tag="OO575_004091"
     CDS             129..1127
                     /gene="yiaK"
                     /locus_tag="OO575_004091"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6666815.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1139..>1283
                     /locus_tag="OO575_004092"
     CDS             1139..>1283
                     /locus_tag="OO575_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6666816.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT          322 a          333 c          324 g          304 t
ORIGIN      
        1 gatttttatg tcttttgaaa ttcatcaatc agattgcttt gttaaaaagt gatcgatata
       61 tttgaaatct agttccgcat attgaaattt taagccaaaa aagcgaccaa aaaacaagga
      121 agcctaggat gaaagtgaca tttgagcagt taaaagcagc ctttaatcgg gtcttaattt
      181 cacgcggcgt tgacagcgaa acggctgacg cctgtgcaga gatgttcgcc cgcaccaccg
      241 aatccggcgt ttattctcac ggcgttaatc gtttccctcg tttcattcaa caactggaaa
      301 acggcgatat cattcctgat gcccaaccca aacgtataac cagcctcggc gcaattgaac
      361 agtgggatgc ccagcgttcg atcggcaacc tgacggcgaa aaagatgatg gatcgcgcca
      421 ttgaactggc tgccgatcac ggtattggtc tggtggcact tcgtaatgcc aaccactgga
      481 tgcgcggcgg cagctacggc tggcaggcgg cggaaaaagg ctatattggc atttgctgga
      541 ccaactccat cgccgtaatg ccgccgtggg gcgcaaaaga gtgtcgcatc ggcactaacc
      601 cgctgatcgt cgccattcct tccacgccga tcaccatggt cgatatgtcg atgtcgatgt
      661 tctcttacgg catgttagaa gttaaccgcc tggcaggccg tcagctcccg gtcgatggtg
      721 gctttgatga tgagggcaat ttgaccaaag aacctggcgt tatcgagaag aatcgccgca
      781 ttttgccgat gggctactgg aaaggttctg gcatgtcgat tgtgctggat atgatcgcta
      841 ctctcctttc cgacggcgca tcggttgccg aagtcaccca ggacaacagc gacgaatacg
      901 gcatttcaca aatttttatt gccattgaag tggacaagct tatcgacggt cccacccgcg
      961 atgccaagct gcaacgtatc atggattacg ttactaccgc tgaacgcgct gacgaaaacc
     1021 aggccatccg cttacccggc cacgaattta ctaccctgct ggccgaaaac cgccgtaacg
     1081 gcatcactgt tgatgacagc gtgtgggcca aaatccaggc gttatgagga gataagtcat
     1141 gatttttgga catatcgcgc agcccaatcc gtgccgtttg cccgccgcca ttgaaaaggc
     1201 gctcgatttt ctgcgcgcta ccaacttcaa cgtcctggag ccgggcgttg tcgaaatcga
     1261 cggcaagaat atttatgcgc aaa
//
LOCUS       DAJTYM010000259         1278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTYM010000259 DAJTYM010000000
VERSION     DAJTYM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(208..609)
                     /locus_tag="OO575_004093"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="OO575_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1038..>1278
                     /gene="virF"
                     /locus_tag="OO575_004094"
     CDS             1038..>1278
                     /gene="virF"
                     /locus_tag="OO575_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6666817.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSI"
BASE COUNT          375 a          251 c          251 g          401 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaaataaa gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt
      961 gtatacaatc actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg
     1021 tatattctgt tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc
     1081 gcttgcataa ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca
     1141 atgaaacttt gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa
     1201 acgtctccat aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa
     1261 atttattatt aagcatta
//
LOCUS       DAJTYM010000260         1265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTYM010000260 DAJTYM010000000
VERSION     DAJTYM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            24..425
                     /locus_tag="OO575_004095"
     CDS             24..425
                     /locus_tag="OO575_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6666818.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            437..808
                     /locus_tag="OO575_004096"
     CDS             437..808
                     /locus_tag="OO575_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6666819.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            795..>1265
                     /locus_tag="OO575_004097"
     CDS             795..>1265
                     /locus_tag="OO575_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6666820.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          282 c          392 g          288 t
ORIGIN      
        1 tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa cgtctgagag
       61 aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca gatattgcgc
      121 agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac atccgggata
      181 agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata tcagcgcagc
      241 cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg gtgacgctgc
      301 ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg ccgggaatgg
      361 cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg gccagaatgc
      421 aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac ttgatctcgc
      481 agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag gccggctaaa
      541 agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg tggccggcgg
      601 tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata ttttacggct
      661 gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc gtatcacccc
      721 tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg gaaacgtaag
      781 ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac agccttgata
      841 cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag aaagcggtct
      901 cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag gtgaaaggga
      961 tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg tcaggaaaaa
     1021 tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg aacacaacac
     1081 ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg gcatttgttc
     1141 agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg cgttttgcaa
     1201 cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag gtagtgaaaa
     1261 tcccg
//
LOCUS       DAJTYM010000261         1199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTYM010000261 DAJTYM010000000
VERSION     DAJTYM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..299
                     /locus_tag="OO575_004098"
     CDS             <1..299
                     /locus_tag="OO575_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666821.1"
                     /translation="VVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCP
                     KPTHMIGGYAQLAWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            296..>1199
                     /gene="narH"
                     /locus_tag="OO575_004099"
     CDS             296..>1199
                     /gene="narH"
                     /locus_tag="OO575_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6666822.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDLYRVGSLTAA"
BASE COUNT          308 a          309 c          340 g          242 t
ORIGIN      
        1 cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc caggaacgca
       61 ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat aactcggtca
      121 cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg gcctggggct
      181 ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc cgcaagatga
      241 agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg aaaaaatgaa
      301 aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct gccatacctg
      361 ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg catggtttaa
      421 caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc aggaagagtg
      481 gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg gcagcaagat
      541 gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg atgattacta
      601 cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca aacatatccc
      661 tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga tctggggccc
      721 aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc gcaacttcga
      781 ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg
      841 cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg gcgctatcta
      901 caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg gctggcgttt
      961 gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg gcaagtcaga
     1021 aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt gctcagaaac
     1081 ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc gcatcgagga
     1141 agcgtcgagc accgagcgcg aagttgacct ctatcgagta ggcagcctga cggctgcgg
//
LOCUS       DAJTYM010000262         1198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTYM010000262 DAJTYM010000000
VERSION     DAJTYM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1143
                     /locus_tag="OO575_004100"
     CDS             <1..1143
                     /locus_tag="OO575_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6666823.1"
                     /translation="ALAGQVGRRVLLIDNGKILGRKILMSGGGRCNFTNLYVEPGAYL
                     SQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLFCDDSAQQIVDMLVDECEKG
                     NVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATGGLSMPGLGASPFGYKIAEQ
                     FGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITAENGTVFRENLLFTHRGLSG
                     PAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPNQSLKNTLAVHLPKRLVERL
                     QQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGYRTAEVTLGGVDTNELSSRT
                     MEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQDLASIVK"
BASE COUNT          270 a          296 c          345 g          287 t
ORIGIN      
        1 gcgctggcag gtcaggtagg acgccgggtt ctgctgatcg ataatggtaa aatactaggg
       61 cgcaaaatcc ttatgtctgg cggtgggcgc tgcaacttta ccaaccttta tgtcgaacca
      121 ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg cactcgcacg ttttacccag
      181 tgggatttca ttgatctggt caataaacac ggtatcgcct ggcacgagaa aacgttaggg
      241 caactcttct gcgatgactc cgcgcagcag attgtcgaca tgctggtgga tgagtgcgag
      301 aagggcaatg taaccttcag attgcgtagc gaagtgctga gtgtggcgaa ggatgaaaca
      361 ggcttcacgc ttgatctgaa cggcatgact gtcggttgcg aaaagctggt catcgcgacc
      421 ggtgggctgt caatgccggg gctgggcgca tcgccgtttg gttataagat tgccgaacaa
      481 tttggcctca acgtgctgcc gacccgcgcg ggtctggtgc cattcactct gcataaaccg
      541 ttgctcgaag agttacaggt gctggcgggc gtggcggtgc cttccgtgat taccgctgaa
      601 aacggcaccg ttttccgtga gaacttactc ttcacccacc gcggcttatc tggaccggcg
      661 gtgttgcaga tttcaagcta ctggcaaccg ggtgaatttg tcagtatcaa tctgctaccg
      721 gatgtggacc tcgaaacctt cctgaatgag cagcgtaacg cacatccgaa tcaaagcctg
      781 aaaaacacac tggcggttca tctaccgaag cggttggttg aacgcttaca gcaactcggg
      841 caaatcccgg atgtttcgct aaaacagctc aacgtgtgtg accaacaggc actgattagc
      901 acattgaccg actggcgcgt acaacccaac ggcactgaag gctatcgcac tgccgaagtg
      961 acgctcggcg gcgtggacac caacgaactt tcttcacgga cgatggaagc gcgcaaagtg
     1021 cctgggctgt acttcatcgg tgaagtgatg gacgttaccg gctggctggg gggctataac
     1081 ttccagtggg cgtggtcgag tgcatgggct tgtgcgcagg atttggcgtc aattgtgaaa
     1141 taaccatgac gttttcccgt cagccgtttg tatatgtcgt tattaagtca ttttggta
//
LOCUS       DAJTYM010000263         1184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTYM010000263 DAJTYM010000000
VERSION     DAJTYM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>1184
                     /locus_tag="OO575_004101"
     CDS             <1..>1184
                     /locus_tag="OO575_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCR6666824.1"
                     /translation="LKMMLEDARPSLLITTDDQLPRFSDVPNLTNLCYNAPLTPQGSA
                     PLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRLLWIQNHYPLTGEDVVAQKTPC
                     SFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFFAEYGVTTTHFVPSMLAAFVAS
                     LTPQTARQSCVTLKQVFCSGEALPADLCREWQQLTGAPLHNLYGPTEAAVDVSWYPAF
                     GEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGVAGDLYLTGIQLAQGYLGRPDL
                     TASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRSDDQLKIRGQRIELGEIDRVMQ
                     ALPDVKQAVTHACVINQAAATGGDARQLVGYLVSQSGLPLDTSALQAQLRETLPPHMV
                     PV"
BASE COUNT          232 a          325 c          351 g          276 t
ORIGIN      
        1 gcctgaaaat gatgctggaa gatgcgcgtc cgtcgctgtt aattaccacc gacgatcaac
       61 tgccgcgctt tagcgatgtt cccaatttaa ccaacctttg ctataacgcc ccgcttaccc
      121 cccagggcag tgcgccgctg caactttcac aaccgcacca cacggcttat atcatcttta
      181 cctctggctc caccggcagg ccgaaagggg taatggtcgg gcagacggct atcgtcaacc
      241 gcctgttgtg gattcaaaat cattatccgc tcacaggcga agatgtcgtt gcccaaaaaa
      301 cgccgtgcag ttttgatgtc tcggtgtggg agtttttctg gccgtttatt gccggggcta
      361 aactggtgat ggctgaaccg gaagcgcacc gcgacccgct cgctatgcag caattctttg
      421 ccgaatatgg cgtaacgacc acgcactttg tgccgtcgat gctggcagca tttgttgcct
      481 cgctgactcc gcaaaccgct cgccagagtt gcgtgacgtt gaaacaggtt ttctgtagtg
      541 gtgaggcctt accggctgat ttatgccgcg aatggcaaca gttaaccggc gcgccgttgc
      601 ataacctgta tggcccgacg gaagcggcgg tagatgtcag ctggtatccg gcttttggcg
      661 aggaactggc acaggtgcgc ggcagcagtg tgccgattgg ttatccggta tggaatacgg
      721 gtctgcgtat tcttgatgcg atgatgcatc cggtgccgcc gggtgtggcg ggtgatctct
      781 atctcaccgg tattcaactg gcgcaggggt atcttggacg acccgatctg accgccagcc
      841 gctttattgc cgatcctttt gctcctggtg aacggatgta tcgtaccgga gacgttgccc
      901 gctggctgga taacggcgcg gtggagtacc tcgggcgcag tgacgatcag ctaaaaattc
      961 gcgggcagcg tatcgaactg ggcgaaatcg atcgcgtgat gcaggcgctg ccggatgtca
     1021 aacaagccgt tacccacgcc tgtgtgatta accaggcggc agccactggt ggtgatgcgc
     1081 gtcagttggt gggctatctg gtatcgcaat cgggcctgcc gttggatacc agcgcattgc
     1141 aggcgcagct tcgcgaaaca ttgccgccgc atatggtgcc agtc
//
LOCUS       DAJTYM010000264         1166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTYM010000264 DAJTYM010000000
VERSION     DAJTYM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(97..360)
                     /gene="rpsT"
                     /locus_tag="OO575_004102"
     CDS             complement(97..360)
                     /gene="rpsT"
                     /locus_tag="OO575_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6666825.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            463..681
                     /locus_tag="OO575_004103"
     CDS             463..681
                     /locus_tag="OO575_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6666826.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            689..>1166
                     /locus_tag="OO575_004104"
     CDS             689..>1166
                     /locus_tag="OO575_004104"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="catalyzes the formation of FMN from riboflavin and
                     the formation of FAD from FMN; in Bacillus the ribC gene
                     has both flavokinase and FAD synthetase activities;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FMN
                     adenylyltransferase"
                     /protein_id="HCR6666827.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCE"
BASE COUNT          264 a          270 c          301 g          331 t
ORIGIN      
        1 caaccggcag tgtgttctct cacgcatcac aaaagcagca ggcataaaaa aacccgcttg
       61 cgcgggcttt ttcacaaagc ttcagcaaat tggcgattaa gccagtttgt tgatctgtgc
      121 agtcaggtta gccttatgac gtgcagcttt gtttttgtgg atcagacctt tagcagcctg
      181 acggtccacg atcggttgca tttcgttaaa tgctttctgt gcagcagctt tgtcgccagc
      241 ttcgatagct gcgtatactt tcttgatgaa agtacgcatc atagagcgac ggcttgcgtt
      301 gtgcttacga gccttttcag actgaatggc gcgcttctta gctgatttga tattagccaa
      361 ggtccaactc ccaaatgtgt tctatatgga caattcaaag gccgaggaat atgccctttt
      421 agccttcttt tgtcaatgga tttgtgcaaa taagcgccgt taatgtgccg gcactcgtta
      481 cgtagtgatg gcgcaggatt ctaccagctt gcggggtgtg aatacagctt ttccgcgata
      541 aaaattgcag caggcggtca gtttcttccc gtgatttgct ccatggcaat gaaaagccac
      601 ttctttctga tttcggtact caatcgccgg ttaaccttga ccgctgtaca aggtatactc
      661 ggacgatttt cactgttttg agccagacat gaagctgata cgcggcatac ataatctcag
      721 ccaggccccg caagaagggt gtgtgctgac tattggtaat ttcgacggcg tgcatcgcgg
      781 tcatcgcgcg ctgttacagg gcttgcagga agaagggcgc aagcgcaact taccggtgat
      841 ggtgatgctt tttgaacctc aaccactgga actgtttgct accgataaag ccccggcaag
      901 actgacccgg ctgcgggaaa aactgcgtta ccttgcagag tgtggcgttg attacgtgct
      961 gtgcgtgcgt ttcgacaggc gttttgcggc gttaaccgcg caaaatttca tcagcgatct
     1021 tttggtgaag catttgcgcg taaaatttct tgccgtaggt gatgatttcc gctttggcgc
     1081 tggtcgtgaa ggcgatttct tgttattaca gaaagctggc atggaatacg gcttcgatat
     1141 caccagtacg caaacttttt gcgaag
//
LOCUS       DAJTYM010000265         1165 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTYM010000265 DAJTYM010000000
VERSION     DAJTYM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..360
                     /locus_tag="OO575_004105"
     CDS             <1..360
                     /locus_tag="OO575_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6666828.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO575_004106"
     CDS             361..726
                     /locus_tag="OO575_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6666829.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..>1165
                     /locus_tag="OO575_004107"
     CDS             723..>1165
                     /locus_tag="OO575_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6666830.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLK"
BASE COUNT          320 a          249 c          330 g          266 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaa
//
LOCUS       DAJTYM010000266         1147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTYM010000266 DAJTYM010000000
VERSION     DAJTYM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..632)
                     /gene="speB"
                     /locus_tag="OO575_004108"
     CDS             complement(<1..632)
                     /gene="speB"
                     /locus_tag="OO575_004108"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6666831.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVD"
     gene            complement(768..>1147)
                     /locus_tag="OO575_004109"
     CDS             complement(768..>1147)
                     /locus_tag="OO575_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666832.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT          283 a          308 c          296 g          260 t
ORIGIN      
        1 tcatccacgc tgcgatcgtt cacctggcag gcgtccagca cggtaaagcc gttgtcttta
       61 tcaaactcgg tacgaatacc aatctgcacg gaatgattcg ggtcgatcag accttctttc
      121 ggcgcggtat agaacatagt gccgtggtca aattcacaac cgttcgcata ggtatcggtg
      181 tgggcgtcaa agtgtaccag cgccattttg ccgaaatgct tcgcatgagc acgcagcagc
      241 ggcagcgtaa caaagtggtc accaccgaag gagagcatac gcttaccggc agccagcagc
      301 ttctcggcgt gcgcctgcag cttttcgctc atctcacggg catcgccaaa ggcatatacc
      361 agatcgccgc agtccacgac gttcagacgc tcacgcatgt cgaaattcca cgggaagcgg
      421 ttgtgttccc aggccagatt cgtcgaaacc tgacggatcg ctgccggacc gtggcgacca
      481 cccgcacgac cagaagtggc catatcgaac ggcacgccag taatcaccca gtctgcatcg
      541 ctgtcatacg gctggaagtt catcggcagg cgtaaaaaac caaaggcatt ggaaaccagt
      601 gagttatcgt attgatgacc taaggtgctc atggatggac tcctattaca aagtcgatat
      661 aaaaaaaccc ttccgcgtcg ttaggcccga cgaggaaggg ttggatttgc gaaacacaag
      721 tttgtgctaa ttatcgcctt aaatgggcga ggcatcaagc gaggttgcta tttccgacac
      781 ggtggcggcg catagccatt gttatctgcc ggaaaggctt gtccagagca cggcggcagc
      841 gcgttttgcg ccgggccttg taattcaaag gcggcaaagc cgattacccc ggtattacgc
      901 acatcgcctt gcgccgaatt ggctgcgtag gaatcagcaa cgttggcgaa ctgaaaagcc
      961 gcttcagtat gtttatcttt gcggaatcct ttaatcacta acgaatctcc ggcattcaca
     1021 atatagccgt tgttattcag cgaaccttgc ttgccgttca gcacatccag gccatcagcc
     1081 gtagcaacga tctcgtagtt ggtgttacga ctgtaattac ggacatataa ttgataattc
     1141 attccga
//
LOCUS       DAJTYM010000267         1145 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTYM010000267 DAJTYM010000000
VERSION     DAJTYM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>1145)
                     /locus_tag="OO575_004110"
     CDS             complement(<1..>1145)
                     /locus_tag="OO575_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666833.1"
                     /translation="LSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAR
                     EIVSRLGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQD
                     TINAFYGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWT
                     THGNVKIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGF
                     TKGEKRRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSN
                     GSVKFSNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTA
                     KNKFIPRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
BASE COUNT          285 a          324 c          239 g          297 t
ORIGIN      
        1 tgcgcagttc ttcacttgct gaggaaaaaa caagcgttcc atccttctcg acatacccat
       61 ccgtttcaag cagaccacac agcatgccta tacgcgttga tttatttgcg gaaaaaaaca
      121 cacgagggat gaatttgttt tttgctgtgc accccattag tccataacct cgcagggtct
      181 ccatgagtgg atttacctga ccacgcgccg ttgatatcag ccagtcctta ccatctccag
      241 agaagttgta atcaggcagt tcagccttca tacgctcgat catgtatggc tcgacgtttg
      301 aaaacttcac gctcccattg ctaagactac catccccgag aagggagccg ataacccatg
      361 ctaatggctc tgagtggtcg ccaaagtcac cagttatttc aggaatccta acgccatttt
      421 tgtgcctctt cgtttcactc aaccgcttca acccgaaggt atctatcacg cggcgtttct
      481 cgcctttcgt gaatccacga ctagtgacct cccataggtg atcgcctgcg caatcaacat
      541 aacgaccatc ttcaaatgtg actcggtaaa catctctaac accctgcggg aatatcccag
      601 tgacagttga cggattgccg tctactgacg ccacctgatc gccaattttt acattcccgt
      661 gagtagtcca tgtcccatca gctaaaagaa taggctcatt cagtgggcat gcctttccgt
      721 gaccgtgcac accattgacc agcgtcaact ctgcctcacg gaactggaat ttatctgcca
      781 gagattccca cggtggatta aacagatact gctgcttgcc gtagaaagcg ttgatagtgt
      841 cctggtaaaa ctctcgcgca ctgtagagtt cttcaggatc gaagtaggat gccgtgccga
      901 tgtactgcca gatttcatcc tcggtaacac cgttcatcag gcattcgttg atgtctttgt
      961 acggcagagt aacaagacgg caacgatgtt caccgagtcg gcttacgatt tcccttgcgg
     1021 cttcacgacc aacatcatca acgtccatcg agatgaatat ttcctcaaac ctgtcgaggt
     1081 tgtgatactc aaactcaatc cactgttgct tagcgccttt cccgccacca aacggcacgg
     1141 ataac
//
LOCUS       DAJTYM010000268         1138 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTYM010000268 DAJTYM010000000
VERSION     DAJTYM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(216..854)
                     /gene="ospI"
                     /locus_tag="OO575_004111"
     CDS             complement(216..854)
                     /gene="ospI"
                     /locus_tag="OO575_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6666834.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1007..1138)
                     /locus_tag="OO575_004112"
                     /pseudo
     CDS             complement(1007..1138)
                     /locus_tag="OO575_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          338 a          279 c          172 g          349 t
ORIGIN      
        1 gacaccgtca tgccaggctc cattgtctgc tgaataatgg caattttttc ctgaggagtt
       61 ttacgtttac gaagctccag ccccagcaaa gttccagtca tctcaaaatt agaactagtg
      121 ttagacatat atccaagcct atctcttatt ttaagataca tccactgtct ggcgtttcaa
      181 ggggccggct caaatctatg tacaggctct cagcatcagc aaagcctctt acttttccaa
      241 ctactactgt tagcatagtg atcaaattcc tgatcagata catgaattac ctgaaccgaa
      301 ctattttcat ctacaaatgc atctggcgat ttttctacag gatcataaag attaaatccc
      361 tttcctgttt gaacatccaa aaacataatt ttttcaccat cgttaaatcc agctatccaa
      421 tgagtggcct gttcaataga aatcattact acacttcctt ttgaagtatt ttgtaaaata
      481 ttttctgcaa tacgctcacg aaatttacta taagtttcat taggatgtga cactatgcct
      541 tcactatcaa tttgctctaa atgcctatga aaaaatccgg tcatatactc tgatatttca
      601 ttaggtacgg gagcctctct tactccatac cctaacatag ccatacaagc atgaagcgca
      661 caccctgaac agttaccatc cccaccagac ggatttatca ttttctgtaa agtcgctgcc
      721 aattcagaat ctttatccat atcaccaaga cttttagagt ttaaagattc cagcaatttt
      781 ttaacattta catttccctc ctctgtatgt gcctcatatc cagcagttcc ctgtaacgac
      841 accccattaa tcatattgta tcctgacagt atatatactc actatatttt cttcataaca
      901 aatatccctc tgatatgaca acaaaaaacg gagccggatt cctgtttttg tgaactgcag
      961 ctattcgtgt ctaaaaagtg atccacttac caccaacggc ctaatatata cagaacggag
     1021 taaacaggac taacagttcc cgacaggtct catcattccg tggcccgaaa gtgtaagcca
     1081 acacaccgcc agttttagtg ttgtacgaat accagagcca gtgttgttgc agccttgc
//
LOCUS       DAJTYM010000269         1128 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTYM010000269 DAJTYM010000000
VERSION     DAJTYM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>1128)
                     /gene="treF"
                     /locus_tag="OO575_004113"
     CDS             complement(<1..>1128)
                     /gene="treF"
                     /locus_tag="OO575_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCR6666835.1"
                     /translation="PVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFTMLG
                     LAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVRG
                     ARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLED
                     VETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFK
                     LESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFS
                     AAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMA
                     IQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIA"
BASE COUNT          249 a          318 c          304 g          257 t
ORIGIN      
        1 tcggcaatat ggtatttctc gatcattttg tgctgttcca gatagaactg attcaccgtt
       61 ttcagccagc tgcgcgcgat ttcatcaccc agaaggtcat cgccatacat tttaaatccc
      121 tgaattgcca tccattgtaa cggtgcccag ccgttgggtt tatcccactg ttcaccggtt
      181 tcgtactcgc ttgccaaaat cccgccaggt gtcagtaacc ggctgcgtac ggcgtttgcc
      241 agacgatcgg cctgttcatg gttcgccatg ccgacataga gcggcacaat ggcggcagcg
      301 gaaaacagcg ccagttgttc gcgtcgccag tcgtaatcgc ggtagatgcc gttttcatca
      361 tcccagaggt aacggtttac cgcatcgcga cgggcactgg ccttctggcg gaacagcgct
      421 tctgtctctt tctcgccttt cagcgccgag atgttggcga tggcgctctc cagtttaaac
      481 aggaaggcat tcagatcgat ggggatgaac tgggtggtac gaatgctcgc cagacgacca
      541 gtatcacgca gccaacggga agagtaatcc caaccggagg ccgctcccgc gcgtaaatcg
      601 cggtacacct cgttgggcgg gcgaccagaa tgtttcgcgg tttcaacgtc ctcaagccag
      661 gattcgtcac gcggcgtgtc gcgatcgtcc cagtaacggt tgagcagcga tccgtccggc
      721 atccgcacaa catggcgata ggcctgatta gggatcaacg attctgcacc gtccatccag
      781 aaggcatatt ccattttaag gtggtcgaga tagcggcgcg caccgcgtac accatcttct
      841 tcaaacaact ccaccatcag cgcaaaaacc ggtggttgcg agcggctcaa ataataggtg
      901 cggttgccgt tggggatgtg accgtagttt tcgatcatcc aggcgaagtt atcggccatg
      961 catttcagca aatcttcccg accactttcc gccagtccca gcatggtgaa ataggaatcc
     1021 caatagtagg tttcgctaaa acggccgccc gggacaatat atgactgcgg tagcgccagc
     1081 agagaagacc acggaatgtg atcctgtggt tcgcgggtta gcaccggc
//
LOCUS       DAJTYM010000270         1118 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTYM010000270 DAJTYM010000000
VERSION     DAJTYM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>1118
                     /locus_tag="OO575_004114"
     CDS             <1..>1118
                     /locus_tag="OO575_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6666836.1"
                     /translation="FRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAEAWR
                     VSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYS
                     AMQKMTNRRHEENWRLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAERE
                     WKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIIN
                     LEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTK
                     PDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEM
                     GGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSS"
BASE COUNT          366 a          244 c          285 g          223 t
ORIGIN      
        1 ttccgcagag gtgaaatatg gtacgccagt ttcacattgc cgaacggtaa aagatttaaa
       61 cagtctcttg gaacaaagga caaaaggcag gcgacagaac tccatgacaa gctaaaggct
      121 gaagcatggc gggtcagcaa acttggtgaa atacctgata taacgttcga ggaagcgtgt
      181 gtcaggtggc ttgaagagaa agcacataaa aaatcactgg acgatgacaa aagccggatc
      241 ggattctggc ttcaacattt cgcaggaatg caactaagag acattactga atcaaaaatt
      301 tattcagcaa tgcagaaaat gacgaaccgg cgtcatgagg aaaactggag actcagggca
      361 gaagcatgca gaaaaaaagg gaaacctgtt ccagaataca cgccaaaacc agcgtccgtt
      421 gcaacgaagg ctacgcatct ttcatttata aaggccctac taagagccgc agagcgtgaa
      481 tggaaaatgc tggataaggc accaattatt aaagtgcctc aaccaaagaa taaacggatc
      541 cgctggctgg agccccatga agcacaaagg ctgattgatg aatgtccgga gccattaaag
      601 tctgttgttg aatttgcact ggcaacaggc ttaagacgct cgaacatcat caaccttgaa
      661 tggcaacaaa tagatatgca gcgccgggtg gcatggataa acccggaaga gagtaaatca
      721 aaccgcgcaa ttggcgttgc gctgaatgat actgcatgtc gcgtattgaa aaaacaaatc
      781 gggaatcatc accgttgggt atttgtgtac aaggaaagct gtaccaaacc agacggaacg
      841 aaagcgccaa cggtcaggaa gatgcggtat gacgcaaaca cagcctggaa agcggcgctg
      901 agacgagcag gtattgatga tttcagattt cacgacttga gacacacctg ggcaagttgg
      961 ctggttcaag ccggagtccc gttgtcagtg ttacaggaaa tgggaggctg ggagtctatc
     1021 gaaatggttc gtcgatatgc tcaccttgca cctaatcacc ttaccgaaca cgcacggcaa
     1081 atagactcga tcctgaaccc atcggtccca aattcgtc
//
LOCUS       DAJTYM010000271         1117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTYM010000271 DAJTYM010000000
VERSION     DAJTYM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..911)
                     /locus_tag="OO575_004115"
     CDS             complement(<1..911)
                     /locus_tag="OO575_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6666837.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFA"
BASE COUNT          324 a          294 c          225 g          274 t
ORIGIN      
        1 gcaaaatcgg caatccgccg catcccggta atgccgcctg catgggtgat cgtcgtgcga
       61 atatagccaa tcagctgctc ttcgattaac tgcttgcagt cccagatact gttaaacact
      121 tcaccgaccg caatcggcgt cacggtatgc tggcgaatca gacgaaaaca ttcctggttc
      181 tcggcaggcg tcggatcttc catccagaac aggcggtaat cctcaatgct cttcccaaag
      241 cgcgccgctt cgatgggggt caggcgatgg tgcatgtcat gcaacagatg ttcgttaaaa
      301 ccgaacttat tacgcaccgc ttcgaataac ttcggcgtaa aatcgagata cttttctgaa
      361 gaccacaact gctcttctgg ccacaggcct ttggttgccg gttcatacgc cagtcctttg
      421 cccttcgcca ggccataagt ggtttgcata cccggtacac cgcactgcac acgaatcgcc
      481 ttaaagccca tctgctggtg ctttgcatag tcttcaagga cttcatcaat cgtacgcccg
      541 gtggtgtggc aataaaccat cacgccttca cgagaagcac caccaagcag ttgatacaat
      601 ggcatatttg ccgctttagc tttgatatcc cacaacgcca tatcaatggc agaaatggct
      661 gacatcgtga cggggccacg tcgccagtaa gcacctttat aaaagaattg ccagatatct
      721 tcgatacggc tggcatcgcg gccaattagt tgtggacaaa gatgatcttt cagataagaa
      781 gcgaccgaca attcgcgacc atttaacgtg gcatctccta atccacaaag tccgctttca
      841 gtggttattt taagcgtaac gaaattacgc cctgggcaag taacaaaaac atctgcagca
      901 ataattttca ttattgcaat ctcctgtgtt gtaaatagac gtttcaaacc taattttacg
      961 cctactacca tacaagagag aaaaagcgat tattcaaccg tgatcacatt aaaaacaaaa
     1021 atttctaccg aggagaaaaa ataccctcaa aaaccacctg aataattaac cattattgtt
     1081 aaaacaacac gttgaataat cacataacaa actccat
//
LOCUS       DAJTYM010000272         1088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTYM010000272 DAJTYM010000000
VERSION     DAJTYM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..987)
                     /locus_tag="OO575_004116"
     CDS             complement(<1..987)
                     /locus_tag="OO575_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR6666838.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHT"
BASE COUNT          331 a          294 c          252 g          211 t
ORIGIN      
        1 ggtgtgaata atgcgcatcg gcgacaaata tcccagacgt accgcctgac gcgtcaatga
       61 gaagacgcca gagataaccg cctgcgaggc aattaccgtc gccagtgcgg cgatgatcag
      121 cagtgggatc agcgcccagt ccggtgccaa caggaagaac gggttcttaa tcgcttccgg
      181 gttctttaac aacaacgctc cctgaccgaa gtaattaagg gtcaaggaag gtaatacgac
      241 ggtgaaccac gccaggcgaa tagagaactt accaaagtgc cccatatcag catacagcac
      301 ctcgcccccc gtaatcgaca gcaccactgc ccctaatgca ataaaagaaa ccgttttgta
      361 ttcaaggaag aaatgcaccg cccacatcgg attcagcgca tgcagcactt ccgggttagc
      421 aataatgcta cgtaacccca gtcctgccag aatcaaaaac caggtcagca tgatcggcgc
      481 aaacagctta ccgaccatag ccgtgccatg cttttgaatc ataaataata aagtgagaac
      541 gataattgag aggggaacta tccaggtatc cagctgcggg gcgacgattt ccagaccttc
      601 aatggctgac atcaccgaaa tagcgggtgt tatgacgact tcaccataga aaaagctgcc
      661 gccgattagc cccataatca ccagcattga tgtggttcgc gccgacgtat tacgcccggc
      721 aagcgacatc aacgtcagga tccccccctc accggcgtta tctgcccgca tcacgaaggt
      781 gagatattta atggaaacca caaagattag cagccagaag atcagcgata aaaagccaaa
      841 cacggcatcg cgttcaacgc caaaaccaaa ctggccggac aaacattcac gaagtgtata
      901 taacgggctg gtaccaatat cgccgtagac aactccaatc gccgcgaggg taatcgcggg
      961 caatgattgc ttattatcag tgctcataga ctagtctttc gttgaaatat gaaatgtgtg
     1021 cttagtccct tggcccacaa aaaagcgcac agtatgcacg attaacggca aaatcgtact
     1081 cctaaatg
//
LOCUS       DAJTYM010000273         1047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTYM010000273 DAJTYM010000000
VERSION     DAJTYM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..869
                     /locus_tag="OO575_004117"
     CDS             <1..869
                     /locus_tag="OO575_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="catalyzes the reduction of trimethylamine-N-oxide
                     to form trimethylamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCR6666839.1"
                     /translation="RNDFDIFRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGR
                     GVHLPAFDDFWNNKEYVEFDHPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMN
                     YDDCQGHPMWFEKIERSHGGPGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGK
                     EPVFINPQDASARGIRNGDVVRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKG
                     GEPGALCKYGNPNVLTIDIGTSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVA
                     QCEYVPASQVKS"
     gene            866..>1047
                     /gene="torD"
                     /locus_tag="OO575_004118"
     CDS             866..>1047
                     /gene="torD"
                     /locus_tag="OO575_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6666840.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENS"
BASE COUNT          246 a          264 c          316 g          221 t
ORIGIN      
        1 cgcgcaacga ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct
       61 ttaccgaagg gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac
      121 aaggcaaagg acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt
      181 acgtcgagtt tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg
      241 atctcgaacc gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg
      301 atatgaacta cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc
      361 acggtgggcc cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc
      421 gacttcactc gcagctatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta
      481 aagagccagt gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg
      541 tggtacgcgt ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct
      601 atgcacccgg cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg
      661 agcctggtgc gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcgggactt
      721 cgcagctggc gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg
      781 gaacagtgga gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat
      841 atgttcccgc gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt
      901 ttacgcctgg ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat
      961 cgccagtgcg cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc
     1021 ggcggtgaat gatctggaaa acagtat
//
LOCUS       DAJTYM010000274         1028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTYM010000274 DAJTYM010000000
VERSION     DAJTYM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO575_004119"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO575_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6666841.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          334 a          151 c          176 g          367 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcaaaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaa
//
LOCUS       DAJTYM010000275         1024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTYM010000275 DAJTYM010000000
VERSION     DAJTYM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>1024)
                     /gene="setB"
                     /locus_tag="OO575_004120"
     CDS             complement(<1..>1024)
                     /gene="setB"
                     /locus_tag="OO575_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6666842.1"
                     /translation="IFLTDEVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKS
                     LIVFCCLLGVLACTLFAWNRNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREA
                     VMFSSFLRAQVSLAWVIGPPLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQK
                     ELPLATGTVEAPRRNRRDTLLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVM
                     MGTAAGLEIPTMLIAGYFAKRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLN
                     AIFIGILGGIGMLYFQDLMPGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVF
                     WFAMVMI"
BASE COUNT          303 a          292 c          252 g          177 t
ORIGIN      
        1 aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg cgacgatgcc
       61 cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga gcgtggtggc
      121 tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc cgcccagaat
      181 gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg aatgcgccat
      241 cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta agaaacgctt
      301 acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca gcccggcggc
      361 ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga taataaatag
      421 cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa tgacaaacag
      481 cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg ccagcggaag
      541 ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa taaacgctac
      601 cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat aagccagcgg
      661 tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga acatcaccgc
      721 ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg ggttagcggt
      781 cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt tgcgattcca
      841 agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca gcgatttgcg
      901 atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc caatgacagc
      961 gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat cggtaaggaa
     1021 aata
//
LOCUS       DAJTYM010000276         1005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTYM010000276 DAJTYM010000000
VERSION     DAJTYM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..1004
                     /locus_tag="OO575_004121"
     CDS             <1..1004
                     /locus_tag="OO575_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6666843.1"
                     /translation="AENYQNWKPATLRENKASHAALIEIFDHLGLNADANRADMLRVR
                     DVLQQLPRNRKQRFKDVPLADLLSREDKTDCLDVVTINNKYLIKMAAVFRWAVRNDLI
                     KKNMTEGLELKVPQRKASGARNAFSTEQVGQLLVAAKAYSQKTSGKPYHYYVTALAAI
                     TGARLNEIAQLQVKDVRTTEAGTVYIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGF
                     ILADFMALVETRRGADGDDAMVFDGLRLMKNGYGEQVSKWFNRTLLPKVLVDRSGLAF
                     HSFRHTVAAQLKQHGVELAYAQAIMGHSSGSITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          259 a          216 c          281 g          249 t
ORIGIN      
        1 aagcggagaa ctatcagaac tggaagcctg caaccttaag ggaaaacaag gcgtcacacg
       61 ctgcgttaat cgaaatattc gaccatctgg gtctcaatgc agacgctaac agggctgaca
      121 tgcttagggt ccgtgatgtg ctccagcagt tacctaggaa ccgtaagcag cgcttcaagg
      181 atgtcccact tgctgacctg ttgagccgtg aagataagac agattgtctg gatgtcgtta
      241 ccatcaacaa caagtatctt attaagatgg ctgctgtctt taggtgggct gtgcgcaatg
      301 acttgattaa aaagaacatg actgagggtc tggagcttaa agtccctcag cgtaaagcct
      361 caggggcccg taacgccttc tctacggagc aggtggggca actactggtc gcagctaagg
      421 catactctca gaagacctct ggtaagccgt atcattatta tgttaccgcc ttggctgcta
      481 tcactggggc gagactcaat gaaatagccc aactacaggt caaagacgtc aggaccactg
      541 aggcgggaac cgtttatatt catatcaatg aagacgatag tagtctaccc ggtaagagca
      601 tcaagaacgc tcatagtgac cgctgtgtgc ctctggtcga tggggcctat ggttttatat
      661 tggctgactt tatggcgctt gtagagaccc gtagaggcgc tgacggtgat gacgctatgg
      721 tgttcgatgg tctgcgtctc atgaagaacg gttacggtga gcaggtgagt aaatggttta
      781 atcggacgtt gttgcctaaa gtcctcgtag accgtagcgg cttagcgttt cactcgttta
      841 gacatacagt tgcggcccag ttaaaacaac atggggtcga gttggcgtat gcacaggcga
      901 tcatggggca tagttcaggg tctattacgt acgaccgtta tgctaaagag gtcgaggttg
      961 ataggctggt caatgtgatg gctgatgttt ataaggagac ctaga
//
LOCUS       DAJTYM010000277         1002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTYM010000277 DAJTYM010000000
VERSION     DAJTYM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..143)
                     /locus_tag="OO575_004122"
     CDS             complement(<1..143)
                     /locus_tag="OO575_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666844.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRD"
     gene            complement(743..991)
                     /locus_tag="OO575_004123"
     CDS             complement(743..991)
                     /locus_tag="OO575_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6666845.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          242 a          185 c          233 g          342 t
ORIGIN      
        1 atgtctcgtt gttcttcttg tatgcggtta tttttccatt cagtccaatg ggttagataa
       61 ttttcatatg cgcggctagt gtcgattgaa taagtggaga acgaatttga aattaatctg
      121 tgattgttat ttgtcgggag cataaatatc agggtgtatt ctttatgttg agaggtgttg
      181 gattcttttt tatctgcttt gcaatgttat tggttccctt cagcagtaga caggaacttc
      241 tggaggaggc ggatatatag tttgtgtaag catattaact ccatatgtta tatattgaag
      301 aacttctgct ttacactatc ctaaactgga tcggtttaat gtaaaaaaac gatacttttt
      361 gggggggggg caatttttcc ttaaggtatg aaaaaaggag ctgaagctat ataatagcta
      421 ttaatgccac tggtatcagt gaatcatgta tataaaaaac tgtgttaagg aggctgtcca
      481 ttagcggttt tttattcttc tggaaccgcg gcatctttgc gtatcttttt gtcttctcac
      541 cgtctggtcg gtgttttgct gagactgcta atttcctgtc tttgctagtg ctgtactggt
      601 actgcccaat catgattggc gggggcgggc ggagttgaga ccgcagacac gtcgtatgca
      661 agaacgtgct gcggctggct ggtgaacttc cgatagtgcg agtattgaat gatttccagc
      721 cgttactgat tttacgtgct aattagtgaa caaaccactc gtcagcagac tcccaggtat
      781 ctttcagagt ttcctgaaca aaagttttag ctgaatcttt atcggcggtg cgcgtaacag
      841 aaaggccatc gttgctggtg gcttttacga tcacctctac atcgtcataa cgtttactga
      901 tgcgtcgggt taattcttcc tttaacgcat ccacagcacc ggttggcatt ttagtcattt
      961 tctctttggc tatgcagatt tcaaaacgca taaaaatccc tc
//
LOCUS       DAJTYM010000278          984 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTYM010000278 DAJTYM010000000
VERSION     DAJTYM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(106..>984)
                     /locus_tag="OO575_004124"
     CDS             complement(106..>984)
                     /locus_tag="OO575_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="HCR6666846.1"
                     /translation="LTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAE
                     ARQGSLLREGMKVVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGM
                     PLHIIDTAGLREASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIA
                     RLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDT
                     NMEGGFLARRRHLQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFT
                     SDDLLGRIFSSFCIGK"
BASE COUNT          217 a          286 c          249 g          232 t
ORIGIN      
        1 ttacgtaaga attttaatgt ttaaaaaagg gttgaggcat aacaggagtg ctaacaatga
       61 agcgaagccg catctgacag tcagaatacg gcttcgtaag cgcggttact taccaataca
      121 gaagctggag aaaatccgcc ccagcaggtc gtctgaagta aattccccgg tgatttcgct
      181 taagttctgc tgtgccaggc gcaactcttc cgccagcagt tcacctgccc aggctcccaa
      241 cagttgcgct ttaccttgct gcaaatgctc cgctgcctgt tccagcgcct gtaagtggcg
      301 acggcgcgcc aggaagccgc cttccatatt ggtgtcaaag cccatgctct gtttgagatg
      361 gttacgcagc acgtccacgc cttcaccagt ccttgccgag agacgaatta acgcgtgacc
      421 gttcacttca ctcattccca gcgtttcgcc ggtgatatcg gctttattgc gtaccacggt
      481 gatcggcagt ttcgcgggca gacgggcgat aaattccggc cagatctctg ccggatcaac
      541 ggcgtctgtc gtggtgccat cgaccataaa cagcacgcgg tcggcctgtt caatttcctg
      601 ccacgcgcgc tcgataccaa tacgttccac ttcgtcactg gcctcacgta gcccggcagt
      661 gtcgatgata tgcagcggca ttccgtcaat gtggatatgc tcacgcagca cgtcacgcgt
      721 ggttccggcg atatcggtta cgattgccgc ttcacggccc gccagcgcgt ttaacaggct
      781 cgatttaccg gcattaggac gtccggcaat cactactttc atcccttcgc gcaacaaact
      841 accctgacgt gcttcagcac gcactgcatc gagatcggcc atcacgtcat taagctgggc
      901 ttcaattttg ccgtcggaga ggaaatcgat ctcttcatca gggaaatcaa ttgccgcttc
      961 gacgtaaatg cgcaagtggg tgag
//
LOCUS       DAJTYM010000279          981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTYM010000279 DAJTYM010000000
VERSION     DAJTYM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..403)
                     /locus_tag="OO575_004125"
     CDS             complement(<1..403)
                     /locus_tag="OO575_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6666847.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKG"
     gene            complement(469..>981)
                     /locus_tag="OO575_004126"
     CDS             complement(469..>981)
                     /locus_tag="OO575_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666848.1"
                     /translation="HYAAIYDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVI
                     TVNSAGEEEHKSDFIASAPDKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKII
                     NLAIRCLKDENTRVMKRIGRCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLR
                     KSLMTGSSRG"
BASE COUNT          244 a          267 c          216 g          254 t
ORIGIN      
        1 cgcccttacg atcaccggca ttcagccgct tataaaacgt cgacgggaaa cacttaccgg
       61 ggccaatgtt ataggggcaa aatgacgcta tacccgcttt ctggggttcg gtcagaggta
      121 ctttaatatt gcgctccacc catgccagcg ccttatcacg ttcaatagcg ttaacctggt
      181 cacatttttc cttcgacagc ttcattcccg gtatgacagg cttaccatcc accattgtgg
      241 caccacgaca gatggtccat atgccagaac catcacggta tgccgttgtg tgattaccct
      301 ctttttcgtt cagaaactgg tcaagtatct ggggagcaga cgcgcctgcg gcaatcagcg
      361 ccagaacagc agccgacagg ccgtatttga tttttgcgct catggatatt tatcaggatt
      421 tatcggcaac aaataacgag ccaacttata tacgcctttt aagataagtc agccccggct
      481 ggatccagtc atcaggctct ttcttaaagg gggagtattg aaatcacgaa gaagagcctc
      541 ccgcaccgca gcatccatgt ctgcaccact ggcaagtgct tcaatctctg ccgccacctg
      601 caaatacccc atgcaacggc caatgcgttt cataactctg gtgttttcgt ctttcaggca
      661 tctgatagcg agattaataa ttttcaggca gtgatgatgc gatgcccgga caggttttcc
      721 gttctcataa acgacgaact caccctcttc acaggaaacc ctgaaaggcc gatggggttc
      781 ctggtcttta tccggtgcag acgcaataaa atcactttta tgctcttcct ctccggcact
      841 gttaaccgta atgacgtact caggccgcag gtaagccgcc tctttttctg acaatgcggc
      901 cggtaaaaac ttttcctgca aaaacttcat aaaagtctcg tgagcgactt tgtggcagta
      961 atcgtaaatc gccgcataat g
//
LOCUS       DAJTYM010000280          963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTYM010000280 DAJTYM010000000
VERSION     DAJTYM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(267..>963)
                     /locus_tag="OO575_004127"
     CDS             complement(267..>963)
                     /locus_tag="OO575_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666849.1"
                     /translation="QQVTEIANQTKSIAIAAKSEQQSQAEARANLALAEAVSAQQNVE
                     TTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAAIVELAEATHKK
                     GLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQVD
                     GLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTS
                     PLISTTPVAENVK"
BASE COUNT          187 a          257 c          242 g          277 t
ORIGIN      
        1 caatcagtat gatgacgagc ttcatcataa ccctttcctt ctgtaaggcc ccttcctcgg
       61 gaggggcttt cccgtttcag ccctttgctg actccccgat tgttgatgag cgccggacct
      121 cgcgcaacgc tttcccagag gcagttttgc cttccccgag cggtcacgca aaacgttgca
      181 acgacctgat catttgcagt ttttagcgga acgcagatat aaaaaatccc cggctttttg
      241 tgccagggac gttcatagct gaaaatttat tttacgtttt cggcgacggg agttgttgag
      301 attaagggtg aagtcaatgc cgccagtgaa ccgcctgaaa cgccaatttc attgagcaag
      361 gagtcaatca gcggagcctg tgtgcggtaa gagagagcgg ctgataatgc ttgttccgct
      421 aggtttccgc cccccacatt acccgtgttt gcatcacccg cagcgctgcc actattcaga
      481 ccatcgacct gaataatctt gataccgtca atagatttca tcggctcaac ggatttttct
      541 attaccgcag gtagcgcctg caacaaggcc agtttgaatt taaggctggt ttgttcatca
      601 gaaagtacgt tgatagcatc gttcagcgca cgttgtgctt ctgcttccgc cagacccttt
      661 ttatgtgtag cttcggctaa ctcaacgata gccgccgcct gcatctctgc ggcttctttt
      721 tctgctttcg cccgcacggt cagttcaacc gctttggttt ctgcatcctg cgcggcagcg
      781 attagggcaa cttgtttagc acgatcggct tctgcggtct ggcgagtggt ttctacgttt
      841 tgttgcgcac ttactgcttc tgcgagtgca aggttagcac gcgcttctgc ctgggactgt
      901 tgttccgatt tggcggcaat agcgatcgat ttcgtctggt tggcgatttc ggtgacctgc
      961 tgc
//
LOCUS       DAJTYM010000281          958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTYM010000281 DAJTYM010000000
VERSION     DAJTYM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..196
                     /locus_tag="OO575_004128"
     CDS             <1..196
                     /locus_tag="OO575_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6666850.1"
                     /translation="MLRFMFGKKRRAYVLNNGGLRMSYVFESALSLAELAIIQSSGAL
                     PSPPGVYVSVVLKESRNEGQ"
     gene            183..>958
                     /locus_tag="OO575_004129"
     CDS             183..>958
                     /locus_tag="OO575_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6666851.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGAL"
BASE COUNT          234 a          219 c          278 g          227 t
ORIGIN      
        1 gatgctgaga tttatgttcg gaaaaaaacg ccgggcttat gttctgaata atggcgggct
       61 gaggatgagt tacgtctttg agtccgcgct ctcgttggca gaactggcga ttatccagtc
      121 gtcgggtgca ctgccatccc cgccgggtgt ttatgtttca gtagttttaa aggagtcccg
      181 taatgaaggc cagtgataaa ccccgccagc tggcggtccc ctttgcgagt accggagata
      241 aaaaccgtat cccggacaag gcgacacagc agaccagaga gagcggtaat gctgcgtatg
      301 attcaggttt tcctccggtg accatgacag cggtctcagc gggaggtata ccgccacacg
      361 gcaaggattt taacggtctg atgtacgata ttaccgcagc aatacggttc gcccaggctg
      421 gcggtttgta cacgtataat gccggttttg cgggggccat tggtggatat gcaaaaggag
      481 ccattctcgc cggagtcgca acaacagcgg tctggctgaa taccacggac gataacctga
      541 ccgatcctga aggctccgac agtgcgggct gggtaaatct tcttgaggat ccgaaaagga
      601 tattcctgcg gcagaagaac aatctgtcag accttcagaa taaagggacg gcacgggata
      661 atcttcaggt ttacagtaaa gagcagtcag atcaacgcta tgttcatcgg gaaggcgata
      721 aaataaccgg agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt
      781 ttggcctgat ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc
      841 agggggaaaa tggcgatatt ggtcccctgc gcccgttcac cattaatttg cggacgggtg
      901 aaatatccat gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgct
//
LOCUS       DAJTYM010000282          952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTYM010000282 DAJTYM010000000
VERSION     DAJTYM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO575_004130"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO575_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6666852.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          284 a          157 c          221 g          290 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 ccccttacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cc
//
LOCUS       DAJTYM010000283          941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTYM010000283 DAJTYM010000000
VERSION     DAJTYM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..105)
                     /locus_tag="OO575_004131"
     CDS             complement(<1..105)
                     /locus_tag="OO575_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6666853.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIG"
     gene            complement(240..845)
                     /locus_tag="OO575_004132"
     CDS             complement(240..845)
                     /locus_tag="OO575_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6666854.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          273 a          214 c          151 g          303 t
ORIGIN      
        1 accaatcaat gcatattcga ttgcagtaac accacgttca tccttcgcga aacgcgcaaa
       61 gcactgttta gttttaccat agtacttagt aataaaagta ttcatgtttt cctcgtatat
      121 tattatttaa attcaaacca ccagcttcat ctgcgtgtca ccggtatccc atatttttgg
      181 gatgtggcta taattatcta ttcccgctat ttagtcttca aaaaaaatta catttaatat
      241 catttatctt gattaagaaa tagtaagact aaaaaaacaa gcgtcgtcag ccagcacact
      301 gctaacacgg taagaacgct aataaaaaca ctccactgct tcgttctata ccattcaata
      361 tttttgtcag tatgttttat tgggagcagc tcttcagtag agaattcctg gctgttatca
      421 ttctcaggaa tgtttgcatc agtgtttact tccctggcct cgctatcgtt ggtatagcta
      481 ctactgtctt catcactgtc acgctcattc tcacagacat gtttctcgac ctttcccaga
      541 tatctgacgc cttttcgcgg aatggtttta attagagaac tcgacaaacc tgcgacgtct
      601 aatgcctttc taagcatgta aatctgccca taataactgc tgtcgcttac cgaacctttc
      661 tgctcccgcc aaacctcatt gataatatgt tgcttatcgg attctccctg tagtaaaagg
      721 agtaaaaatc gatagccgtt ttcagacaga ttgattacat tgcctgtact ggtggtgata
      781 cgtcgagacg ctgggtaaaa cttaactccc tgccctaaag aaaaataaaa acggtcatcg
      841 ctcataagta ccgacccctg ttttcttctt cacatgacta ctcattacaa ctttgtatga
      901 agtaagatgc aaaatcatat tgtttacatt aagttaagca a
//
LOCUS       DAJTYM010000284          901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTYM010000284 DAJTYM010000000
VERSION     DAJTYM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(520..>901)
                     /gene="fabY"
                     /locus_tag="OO575_004133"
                     /gene_synonym="yiiD"
     CDS             complement(520..>901)
                     /gene="fabY"
                     /locus_tag="OO575_004133"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6666855.1"
                     /translation="ITTMPETGNQNPHHTLFAGSLFSLATLTGWGLIWLMLRERHLGG
                     TIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQMQVEIFGDETPGAV
                     FEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT          261 a          208 c          192 g          240 t
ORIGIN      
        1 tgatttaata gcgtttgctt cggtgattta ttactcatta gttgtttttc taaattaaca
       61 acagaataac tttcttgttt aatgagatta atattggcaa ccattatagc ctccttccca
      121 cggtttaaga gcaaacatac aacataatga ttcataacat acagagcagt aaatgaatga
      181 tactgacaat gtatgccagc ccattatctt tgcataatat ttgtgagcta tagattttaa
      241 aaatatttat gaaaaaagta tggcaccaga gggggttttg actatcaaaa atcgctcaaa
      301 cctgaactaa tatgatgagt taagcattat ctgcaataat tgccactcag ctctacaaat
      361 aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggtgcca acgaagaata
      421 aaaatttata aagttcttat tcaatataac cggaatagtc agggactgat gtaaatgacc
      481 ggaatgaatg agagaggcag caccgcccct ctccataagc tactcttctt cgttcccgcc
      541 ctcttcatac gggccaaatg gcttcgcggg cagaacgata tacgtgcctt caaacactgc
      601 acccggcgtc tcgtcgccaa agatttcgac ctgcatctgc acccgtgctt ttcgtccgcg
      661 cgccagacgg tcgagatcgc cgcttaaggc accaaggtcg gctaccgcat ggggtttacc
      721 gctaatcggt ttgctgtagc ggatatgcgc atccgccaga ataatcgttc cgccgaggtg
      781 gcgttcacgc agcatcagcc agataagccc ccaaccggtg agcgtcgcca gcgagaataa
      841 actcccggca aacagcgtat ggtgcggatt ctgattgccg gtttccggca tggtggtgat
      901 a
//
LOCUS       DAJTYM010000285          870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTYM010000285 DAJTYM010000000
VERSION     DAJTYM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>870
                     /locus_tag="OO575_004134"
     CDS             <1..>870
                     /locus_tag="OO575_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6666856.1"
                     /translation="LRFNIINDKENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQ
                     LFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAANALMAELA
                     TIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEET
                     YQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYA
                     NKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGAKVILAKSAGEEQK
                     LQIIAVSNKGDLS"
BASE COUNT          263 a          192 c          210 g          205 t
ORIGIN      
        1 acctgcgctt taatatcatt aatgataaag aaaaccgggt gaacggcatc gcactctatt
       61 atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt tgtcgaacaa gctgaatgga
      121 gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg catacagtcg ggcgagttga
      181 agactatttc tggcggcact gcgcgcagcg ttaaaattgc acccgattat cagtcgctgt
      241 ttttccgtgt aaatgcacga gacgataata tgcaggatgc tgcgaatgca ttaatggcag
      301 agttggcaac cattgatcag catggttttt ctgctgaaga actcgatgat gtcaaatcta
      361 cccgcctcac ctggctgaaa aatgcggttg atcagcaagc tgaacgtgat ttacgtatgc
      421 tgaccagtcg cctggcatcc agctcattaa ataatacgcc gttcttgtcg ccggaagaga
      481 catatcaact ttcgaaacgt ctgtggcagc aaattaccgt gcaaagtctg gcggaaaaat
      541 ggcagcagtt aagaaagaac caggacgcat tttgggagca aatggtaaac aatgagcttg
      601 ccgccaaaaa agcattgtct cctgcggcta tcctggcgct ggaaaaagag tacgccaaca
      661 aaaagctggc ggcttacatc ttcccaggca gaaatttatc gttaacagta gacgctgacc
      721 cacaggcgga aattagcagc aaagaaacgc tggctgagaa tctgacatca ttaacacttt
      781 ccaatggtgc caaggttatt ctggcaaaat ccgcgggtga agagcaaaag ctacaaatta
      841 ttgccgtatc gaataaaggc gatttaagtt
//
LOCUS       DAJTYM010000286          863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTYM010000286 DAJTYM010000000
VERSION     DAJTYM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..86)
                     /locus_tag="OO575_004135"
     CDS             complement(<1..86)
                     /locus_tag="OO575_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6666857.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVT"
     gene            251..>863
                     /gene="adeP"
                     /locus_tag="OO575_004136"
     CDS             251..>863
                     /gene="adeP"
                     /locus_tag="OO575_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6666858.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIH"
BASE COUNT          183 a          210 c          216 g          254 t
ORIGIN      
        1 atcgttacat atgcgcggga gcaaatgact tcactgtcga ccagcgtacc gtcgcagtcg
       61 aaaaataccg cttctatccg ggacatgcct ttccttttta atcagtttaa cgttttcgta
      121 gagccgattt gcgcaacgca atcgttgccg tataagtaaa tccaataaaa aagtctctca
      181 caaacgcgcc tattgtcgca ttttggtata cgatagcgac ggattttccc tccttgttcg
      241 gaaatagata atgagtcatc aacataccac ccagacttct ggtcagagga tgctggaacg
      301 tgtttttaaa ctgcgcgaac atggtactac ggcacgaact gaagtgattg ccgggttcac
      361 caccttcctg acgatggtct acatcgtatt tgttaaccca caaattctgg gcgttgctgg
      421 catggacacc agtgccgtct tcgtaacaac ctgtctgatt gctgctttcg gtagcattat
      481 gatgggattg tttgcaaacc ttccggtggc gctggctcct gcgatggggc ttaacgcctt
      541 tttcgctttt gtggttgtcc aggcaatggg gctgccgtgg cagatcggta tgggcgcaat
      601 cttctggggc gctgtcggac tgcttctttt gaccattttc cgcgttcgtt actggatgat
      661 tgctaacatt ccggtaagtc tgcgtgtggg tattaccagc ggtatcggcc tgtttatcgg
      721 catgatgggg ctgaaaaacg caggtgtgat tgtcgctaac tcggaaacgc tggtgagcat
      781 cggtaatctg acttctcaca gcgtacttct gggtatcctc ggctttttca tcattgctat
      841 tctggcctcg cgcaacattc acg
//
LOCUS       DAJTYM010000287          860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTYM010000287 DAJTYM010000000
VERSION     DAJTYM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..139)
                     /locus_tag="OO575_004137"
     CDS             complement(<1..139)
                     /locus_tag="OO575_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="with EfeUB forms an iron transport system which is
                     silent in E. coli K-12 due to a mutation in EfeU; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter"
                     /protein_id="HCR6666859.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TI"
     gene            complement(197..>860)
                     /locus_tag="OO575_004138"
     CDS             complement(197..>860)
                     /locus_tag="OO575_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6666860.1"
                     /translation="NETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQ
                     LEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGL
                     ATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIA
                     FDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASR
                     SA"
BASE COUNT          228 a          246 c          216 g          170 t
ORIGIN      
        1 taatggtcat cggttcgcac tgcttatccg tcacggtcac tttgacctgc ggcacatcag
       61 cggcgttagc cataaaagca gaagaaaaca gcgcagccac gctcaactgc aatgcgttac
      121 ggcggaagtt aatggtcatg acgagactat ccctttaaag tatgttgtaa ctaagcaaga
      181 gtttgcgtcg tatttgttac gcggagcgag acgctgtcgc ccctgcgcgt ggtggcagag
      241 caaatgccac cagcgccggg atgagataaa taaaccagac ggcgacttcg ctgacgctcg
      301 gtgcttcctg atagccaaaa atcccttcca tcagcgtgcc aaacagcgag tgagttgaga
      361 ggaccgcgct catatcgaag gcgatttcct gaaagtggtt ccacaatccg gcttcatgaa
      421 atgcgcgaat ggcaccagcc gccagccctg cggcgacgaa gagaataaac aggctggtcc
      481 atttaaaaaa tgcaccaaga ttgaggcgaa taccgcccca gtagagcagg aagccgagca
      541 ccacggcagt agcaagaccg agcattgcac ccagcggcgg ccagatcccg acatcttgtt
      601 gaaatgccgc cagcaggaaa aagaccgact ccagcccttc ccttgcaacg gcaaaaaaga
      661 ccatcatcac cagcgcccag ccatgatgat ttccacgctg caatgcgcta tcgactgcct
      721 gttccagttg cactttgacg ttgcgcgaca ctttgcgcat ccagaaaacc atccaggtaa
      781 ggatcaccac ggcgatcacc gccacgatac cttcaaacag ttcctgctct ttttgcggaa
      841 attcgccggt ggtttcgtta
//
LOCUS       DAJTYM010000288          859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTYM010000288 DAJTYM010000000
VERSION     DAJTYM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..601)
                     /gene="dkgB"
                     /locus_tag="OO575_004139"
     CDS             complement(<1..601)
                     /gene="dkgB"
                     /locus_tag="OO575_004139"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6666861.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHN"
     gene            complement(764..840)
                     /locus_tag="OO575_004140"
     tRNA            complement(764..840)
                     /locus_tag="OO575_004140"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Asp,seq:gtc)
BASE COUNT          230 a          198 c          193 g          238 t
ORIGIN      
        1 cattgtgttt agctgcaata cgagcaataa cctcatcttt cagtgcctta ccataagcca
       61 gcgtcatata ggaagtgata tgaataccgt gctgtttagc ccaggcaacc actttacggt
      121 tttgcagata aggagagagt tcaatctggt tggtagcgat gttttcagcg ccaacagcag
      181 caatcacttt ttccatcaat gggatcgtga agttggaaat accgatctca cgcgtcagcc
      241 cttgtttttt ggcttccagc agcgcctgca taaactcttc aacagagact tcatcgtttg
      301 gtgacggcca gtggattagc gtcagatcaa cataatcggt acgcaatttt tgcaggctct
      361 ctttcagact cgggatcaat ttgtctttgc tgagattttc aatccagatt ttagtggtga
      421 tgtagagttc atgacgtggc acgccacttt ctgcaatcgc ctgacctact gcggcttcgt
      481 tatcatagat ttgtgcggta tcaattgcgc gataaccaag ttcaagcgcc gttttcacag
      541 atgaaataac aacgtcgtct ttcagacgga aagtacctaa accaaatgca gggatagcca
      601 taatatgcct cttttaatgc tattttcgtt aactgaggtg attatgggct tcagactgtt
      661 gcgggaaaag agcgtaaaac gcagaggatt tttgcgattc tagcaataat agatatccag
      721 acgcaaaaaa acatcgagcg ttaactcgag gcttcttaat aagtggcgga acggacggga
      781 ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc
      841 accgaattct tttacaacc
//
LOCUS       DAJTYM010000289          832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTYM010000289 DAJTYM010000000
VERSION     DAJTYM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(46..121)
                     /locus_tag="OO575_004141"
     tRNA            complement(46..121)
                     /locus_tag="OO575_004141"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85..87),aa:Glu,seq:ttc)
     gene            complement(207..>832)
                     /locus_tag="OO575_004142"
     rRNA            complement(207..>832)
                     /locus_tag="OO575_004142"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          183 a          241 c          201 g          207 t
ORIGIN      
        1 ttttgagata tcaaggcagg tgactttcac tcacaaacca gcaagtggcg tcccctaggg
       61 gattcgaacc cctgttaccg ccgtgaaagg gcggtgtcct gggcctctag acgaagggga
      121 cgtatcagtc tgcttcgcaa gacgccttgc ttttcacttt ctatcagaca atctgtgtga
      181 gcactacaaa gaacgcttct ttaaggtaag gaggtgatcc aaccgcaggt tcccctacgg
      241 ttaccttgtt acgacttcac cccagtcatg aatcacaaag tggtaagcgc cctcccgaag
      301 gttaagctac ctacttcttt tgcaacccac tcccatggtg tgacgggcgg tgtgtacaag
      361 gcccgggaac gtattcaccg tggcattctg atccacgatt actagcgatt ccgacttcat
      421 ggagtcgagt tgcagactcc aatccggact acgacgcact ttatgaggtc cgcttgctct
      481 cgcgaggtcg cttctctttg tatgcgccat tgtagcacgt gtgtagccct ggtcgtaagg
      541 gccatgatga cttgacgtca tccccacctt cctccagttt atcactggca gtctcctttg
      601 agttcccggc cggaccgctg gcaacaaagg ataagggttg cgctcgttgc gggacttaac
      661 ccaacatttc acaacacgag ctgacgacag ccatgcagca cctgtctcac ggttcccgaa
      721 ggcacattct catctctgaa aacttccgtg gatgtcaaga ccaggtaagg ttcttcgcgt
      781 tgcatcgaat taaaccacat gctccaccgc ttgtgcgggc ccccgtcaat tc
//
LOCUS       DAJTYM010000290          825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTYM010000290 DAJTYM010000000
VERSION     DAJTYM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            404..825
                     /gene="ospC4"
                     /locus_tag="OO575_004143"
                     /pseudo
     CDS             404..825
                     /gene="ospC4"
                     /locus_tag="OO575_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
BASE COUNT          288 a          145 c          119 g          273 t
ORIGIN      
        1 tgcagaccat ctgccattat accgccaaag tgatctacta ttccacacag ctatttagct
       61 ctctttttgt tgagctccca tgattctttc atttattgtt actaattatt ttatgttatc
      121 atgatgttag aacacgctct gctctgtgtc tctattttat ctatcatatt ctgaggatta
      181 actacataac aaggcgcaag atatatatac aaaaacaaat aataccgttg caataattcc
      241 ttttagtaaa ggaggtgtga ttaatttcag gggatttcag agtaaaaatt gtacaacata
      301 ttaaaatatg ttcatttcgt tataatacac acaaggaaat attaagttag ataatatatt
      361 ctaaataacc atagataaaa acgcacataa tggggaatat tttatgaaaa tacctgaagc
      421 agtaaatcat attaatgttc agaataatat tgatcttgtt gatggaaaaa cgaatccgaa
      481 taaagcaaca aaagcattac aaaaaatata ttacgcgtaa caaactcatc ttcttctggc
      541 ataagtgaaa aacatttaga tcactgtgct aatactgtga aaaacttttt aaggaagtct
      601 atagcagcgc aaagttatag caaaatgttt tctcaaggga ccagtttcaa atcactaaat
      661 ctttctcttg aagcgccatc tggagcacgg tcatcatttc ggtcacttga gcatctcgat
      721 aaggtgtcca ggcactatat ctctgagata atacaaaaag ttcatcctct ttcttcagac
      781 gaacgtcact tactttctat tattattaat tctaatttta acttc
//
LOCUS       DAJTYM010000291          823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTYM010000291 DAJTYM010000000
VERSION     DAJTYM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            33..823
                     /locus_tag="OO575_004144"
                     /pseudo
     CDS             33..823
                     /locus_tag="OO575_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          212 a          206 c          219 g          186 t
ORIGIN      
        1 agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat cccgaagagt
       61 ttaaaactga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc agcgttgcaa
      121 cacgtctcga tatcaccacc cacagccttt atgcctggat aaagaagtac ggtccggatt
      181 cttccactaa taaagaacag tcagatgctc aggccgagat ccgccgtctc cagaaagagc
      241 tgaaacgggt taccgacgaa cgggacatat taaaaaaagc cgcggcgtac ttcgcaaagc
      301 tgtccgactg aggtacgcct ttatccgtga caactcctgt tgctggcctg ttcgcctgct
      361 ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc tggcttcagc agccgcattc
      421 acaacgccat caggcagacc tgagactgac aggacagatt aaacagttct ggctggaatc
      481 gggatgcgtc tatggttatc gcaaaatcca tctggatctg cgtgacagcg ggcaacagtg
      541 cggagtaaac agagtctgga gactgatgaa acgtgtcgga ataaaggctc aggtcggata
      601 ccgaagcccg cgggcacgta aaggcgaggc cagtatcgtg tcacccaaca ggctccagcg
      661 acagttcaat ccggatgctc ctgatgagcg ttgggtaacg gacataacct acatcaggac
      721 ccacgaaggc tggctgtatc ttgccgttgt tgttgatctg ttctcacgca aaattatcgg
      781 ctggtccatg caatcccgga tgacaaagga cattgtcctg aac
//
LOCUS       DAJTYM010000292          814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTYM010000292 DAJTYM010000000
VERSION     DAJTYM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            369..572
                     /locus_tag="OO575_004145"
                     /pseudo
     CDS             369..572
                     /locus_tag="OO575_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            591..>814
                     /locus_tag="OO575_004146"
     CDS             591..>814
                     /locus_tag="OO575_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666862.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTG"
BASE COUNT          216 a          145 c          223 g          230 t
ORIGIN      
        1 aataaaaagc ttatagattt atagttgata attaaaacgc atccgatatt tcagcttttt
       61 ctatgcaaga tacaatttta taaggtgtcc atttttatat ggaggcggta gcgtgtctgt
      121 tactagtggt tttatttgca tagtgaatat attgggattg ttaaatacat aactatgtat
      181 ccgctctggt cgttgtaaat attgctaatt gcttaatatt attagtgatt atgactggaa
      241 agataaagtg tgctgtaaag cggtgatggg tattacattc atgattaaaa aaacagaacc
      301 tgtcaataaa taattccttt attaaaacgg actatttaca ttatgacgga atattatctc
      361 aacgaggcgg tttggaaata cagagccagt ttctccgggg taacgagcag gtgtaccagt
      421 gtcaggtggc atttgtattg ccgggtgagc gggtcatgat ggccttcacc tacgccagaa
      481 ccacaccgct gaccccggca gatatgacgc gctgggctga aattaaaaag aatctgcgct
      541 tcaggatgcg gcaggaagtc aggacgaatt aatcatacag ggaacgggac atggaagagg
      601 cattctgggc ggcaagacag ggtgatgccc tgctgcatac gtcgtttatg gcagatgtgc
      661 tgggggcggt gctggaggtg gccgcaaatg tcgttattga tgccctcatt gtcggcacaa
      721 cctcgttact ggcgacgtca gtggggatca cgatggggtg cacggccgtc gtgctgaccg
      781 gttttgtggc gggtgtggcg atggtgtaca ccgg
//
LOCUS       DAJTYM010000293          814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTYM010000293 DAJTYM010000000
VERSION     DAJTYM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(68..733)
                     /locus_tag="OO575_004147"
     CDS             complement(68..733)
                     /locus_tag="OO575_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6666863.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          213 a          204 c          182 g          215 t
ORIGIN      
        1 tcaagcaccc gcagtcattc tacttacctc gccaatatga aatcaatcag aaaggtgccc
       61 cataaaatca ctccttctct tctttaccgt agtggagttg accaattttg ataagagggc
      121 gtccctgaga tttgcggtgt agattggtat cgcgcagaga atacacacag ccacaatatt
      181 cctgctgata gaatttttcg cgcttgctga tttcaatcat acgggacgag ccgccctgct
      241 tgcgccagtt ataatcccag tacaccatgc ccggataatg cgcgacggct cgccgcccac
      301 agtcgttaac ctgctgcata tttttccagc gtgaaatgcc cagtgaactg ctgatcacac
      361 tgaaaccatt ttcagcggcg tacagcgctg tccgctcaaa acgcatgtca aaacacatgg
      421 tgcaacggat ccctcgttcg ggctcccatt ccattccttt ggctcgttca aaccagttgt
      481 ctgtgtcgta atcagcatca ataaacggca cgccgtgttg ttcagcaaag cgaatattct
      541 catccttacg aattaaatac tctttctgag gatgaatgtt cgggttgtag aaaaagatgg
      601 tgtagtcgat ttccgaggcc tgaagcgcct ccatcacctc accggaacat ggagcacagc
      661 aagagtgcag tagtagtttg tttgccccgt ttgggagctc caatttgggc cgtttgaaat
      721 cagcaactgt cataaatatg tttattgagg tcataaaaat atcaaagagt gtagcattag
      781 aacggggcta tcggaaacag atgtgtgact gctc
//
LOCUS       DAJTYM010000294          800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTYM010000294 DAJTYM010000000
VERSION     DAJTYM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            56..724
                     /gene="mcbR"
                     /locus_tag="OO575_004148"
     CDS             56..724
                     /gene="mcbR"
                     /locus_tag="OO575_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6666864.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVV
                     ENLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLW
                     IRMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQ
                     QYNR"
BASE COUNT          256 a          168 c          180 g          196 t
ORIGIN      
        1 gaactccttc tgccatgcag gcagggttgg acagaaaaca cgcgaaggaa ttaaaatgcc
       61 tggaacggga aaaatgaaac atgtcagttt gactctgcag gttgagaacg acctgaaaca
      121 tcagcttagt attggcgcat taaaacctgg cgcacgcctg attactaaaa atctggcgga
      181 gcaattaggt atgagtatta cacctgtgcg tgaagcatta ttacgtctgg tttcggtgaa
      241 tgcgctttct gtcgcacctg cacaagcatt tacagtaccg gaagtgaggc aaagtcagtt
      301 agacgaaatc aatcggatcc gctacgaact ggaattaatg gcagttgccc tggctgttgt
      361 tgaaaacctc tcctcgcaag acatagcaga acttcaggaa ttgctcggga aattacagca
      421 ggcgcaagaa aagggcgaca tggaacaaat cattaatgtg aacaggctat ttcgcttagc
      481 gatttatcat cgctcaaata tgcccatcct gtgtgagatg attgagcaac tgtggatcag
      541 gatgggacct gctttacatt atctttatga agcgattaac ccagcggaat tacgggagcg
      601 catagaaaac tatcatctat tactcgctgc gttaaaagca aaagacaaag agggatgcaa
      661 acattgtctt gctgaaatta tgcaacaaaa tattgctatt ttataccagc aatacaatcg
      721 ttaaattaaa tgcccggcaa ataccgggca tcaagtaatt actcaaatcg ccacgcaata
      781 ttcatgccga caccataatt
//
LOCUS       DAJTYM010000295          798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTYM010000295 DAJTYM010000000
VERSION     DAJTYM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>798
                     /locus_tag="OO575_004149"
     CDS             <1..>798
                     /locus_tag="OO575_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR6666865.1"
                     /translation="LAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIM
                     LPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFW
                     RTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQ
                     LIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLV
                     KLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT          196 a          229 c          226 g          147 t
ORIGIN      
        1 cttgccgaat caggtactgt aacgctacgc aagcgccgtc ttcccctcga aatgatggtc
       61 tggtgtattg ttggcatggc gcttgagcgt aaagaacctc ttcaccagat tgtgaatcgc
      121 ctggacatca tgctgccggg caatcgcccc ttcgttgccc ccagtgccgt tattcaggcc
      181 cgccagcgcc tgggaagtga ggctgtccgc cgcgtgttca cgaaaacagc gcagctctgg
      241 cataacgcca cgccgcatcc gcactggtgc ggcctgaccc tgctggccat cgatggtgtg
      301 ttctggcgca caccggatac accagagaac gatgcagcct tcccccgcca gacacatgcc
      361 gggaacccgg cgctctaccc gcaggtcaaa atggtctgcc agatggaact gaccagccat
      421 ctgctgacgg ctgcagcctt cggcacgatg aagaacagcg aaaatgagct tgctgagcaa
      481 cttatagaac aaaccggcga taacactctg acgttaatgg ataaaggtta ttactcactg
      541 ggactgttaa atggctggag cctggcggga gaacaccgcc actggatgat acctctcaga
      601 aagggagcgc aatatgaaga gctcagaaaa ctgggtaaag gcgatcatct ggtgaagctg
      661 aaaaccagcc cgcaggcacg aaaaaagtgg ccgggactgg gaaatgaagt gacagcccgc
      721 ctgctgaccg tgacgcgcaa aggaaaagtc tgccatctgc tgacgtcgat gacggacgcc
      781 atgcgcttcc ccggagga
//
LOCUS       DAJTYM010000296          795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTYM010000296 DAJTYM010000000
VERSION     DAJTYM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            167..>795
                     /locus_tag="OO575_004150"
     CDS             167..>795
                     /locus_tag="OO575_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HCR6666866.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFR"
BASE COUNT          199 a          212 c          190 g          194 t
ORIGIN      
        1 accagccagc tctgccataa cttgccacat tgaaaaagta gccaaagtgc ttcaggcacc
       61 atcagttcac catcaccaaa aaaaagaaac attccagaac ttccgcatct cgcccagccg
      121 ctccgacttc tatactgaat agaaaacgtc aacttaagag aaacctatgc cattacccga
      181 ttttcatgtt tctgaacctt ttaccctcgg tattgaactg gaaatgcagg tggttaatcc
      241 gccgggctat gacttaagcc aggactcttc aatgctgatt gacgcggtta aaaataagat
      301 cacggccgga gaggtaaagc acgatatcac cgaaagtatg ctggagctgg cgacggatgt
      361 ttgccgtgat atcaaccagg ctgccgggca gttttcagcg atgcagaaag tcgtattgca
      421 ggcagccgca gaccatcatc tggaaatttg cggcggtggc acgcccccgt ttcagaaatg
      481 gcagcgtcag gaggtatgcg acaacgaacg ctatcaacga acgctggaaa actttggcta
      541 tctcatccag caggcgaccg tttttggtca gcatgtccat gttggctgtg ccagtggcga
      601 tgacgccatt tatttgctgc acggcttgtc acggtttgtg ccgcacttta ttgccctttc
      661 cgccgcgtcg ccatatatgc agggaacgga tacgcgtttt gccccctcac gaccgaatat
      721 tttttccgcc tttcctgata atggcccgat gccgtgggtc agtaactggc aacaatttga
      781 agccctgttt cgctg
//
LOCUS       DAJTYM010000297          788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTYM010000297 DAJTYM010000000
VERSION     DAJTYM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..305
                     /gene="ssuA"
                     /locus_tag="OO575_004151"
     CDS             <1..305
                     /gene="ssuA"
                     /locus_tag="OO575_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6666867.1"
                     /translation="EKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIA
                     SYLDHRPPTTIKPVNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            302..>788
                     /locus_tag="OO575_004152"
     CDS             302..>788
                     /locus_tag="OO575_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6666868.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KL"
BASE COUNT          182 a          216 c          216 g          174 t
ORIGIN      
        1 cagaaaaaaa cggcgctttt attcagggcg tactggcaac ctttagtgag gccgatgcgt
       61 taacccgcag ccagcgcgaa caaagcatcg ctttactggc aaaaacgatg ggcttaccgg
      121 caccggtgat tgcctcttac ttagatcatc gccctcctac caccatcaaa ccggttaacg
      181 ccgaggttgc cgccttacag cagcaaacgg cagatctgtt ttatgaaaac cgtctggtgc
      241 cgaaaaaagt cgatattcgc cagcgcatct ggcagcccac tcaactggaa ggaaaacaat
      301 tatgagtctg aatatgttct ggtttttacc gacccacggt gacgggcact atctgggaac
      361 ggaagaaggt tcacgcccgg ttgatcacgg ttatctgcaa caaattgcgc aagcggcgga
      421 tcgtctgggc tataccggtg tgctgatccc aacggggcga tcgtgtgaag atgcgtggct
      481 ggttgcagcg tcgatgatcc cggtgacgca gcggctgaag tttcttgtcg ccctgcgtcc
      541 cagcgtaacc tcacctactg ttgccgcccg ccaggccgcc acgcttgacc gtctctcaaa
      601 tggtcgtgcg ttgtttaatc tggtcacagg cagcgatcca caagagctgg caggcgacgg
      661 cgtgttcctt gatcatagcg agcgctacga agcctcggcg gaatttaccc aggtctggcg
      721 gcgcttattg ctgggggaaa ccgtcaattt caacggtaaa catattcatg tgcgcggagc
      781 aaaactgc
//
LOCUS       DAJTYM010000298          788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTYM010000298 DAJTYM010000000
VERSION     DAJTYM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..598)
                     /locus_tag="OO575_004153"
     CDS             complement(<1..598)
                     /locus_tag="OO575_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR6666869.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRAL"
BASE COUNT          199 a          207 c          202 g          180 t
ORIGIN      
        1 tgagagcgcg ctgggtatcc atcagacaca ggcgaacttt ccagccgata tcttccagtt
       61 ccgccagcgt ggagagcgcc tcgtcgtact catcgccctg atgcccttcc ataatcaccc
      121 ggctcaactg ctccaggtcg ctatagatat tttcaatttc atctgccaac tgttcgattt
      181 tggtttcgaa cagatccagc agcaactcgt aggcgttacc gtctaccatc gactggctac
      241 gggcacgcat acgatacaga cgaaaagcgg gcagttcacg ctcacgcaga gtaaacagac
      301 gaccatcacg gatggtaaat gccacagtgg agttaccggc gtgatcttcc gcatcttcaa
      361 agaagaagaa ggagtgaata tgcaggccgt cgtcgtcttc aaagaaacgt gccgatgctt
      421 cgatgtcttc cagttccggg cgggttgcca ggctctggcc aagttcagat tgtacgcgca
      481 gtcgctcgtc gtcgtccggt tcgacaagat caatccatac tgcatttaca aggggttgtg
      541 actcttcgac ttccagccgg gtcagtcggt tattttccag ttgaaatgcg ctcagcatga
      601 ccgggactcc caatgcttaa aatatcggac agttcggtgg gcacacagaa acaaattggg
      661 tttcagacca ttaaacagcc tgactcagcg cgacgggaaa aattgaggtc gctgacaacc
      721 gctaaggcta tcagcaaaaa gggatagcct taggagttga tcctggatga caggatagtg
      781 agccagta
//
LOCUS       DAJTYM010000299          785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTYM010000299 DAJTYM010000000
VERSION     DAJTYM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..180)
                     /locus_tag="OO575_004154"
     CDS             complement(<1..180)
                     /locus_tag="OO575_004154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR6666870.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
     gene            complement(189..>785)
                     /locus_tag="OO575_004155"
     CDS             complement(189..>785)
                     /locus_tag="OO575_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6666871.1"
                     /translation="QELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAYGRKIRFGIR
                     LHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAALHNGKRDNLE
                     ISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPHLEEAYRVGE
                     LLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
BASE COUNT          163 a          249 c          208 g          165 t
ORIGIN      
        1 ccacagttcg ccgctggcgg agagcgtcca gaacgccgtc accactcctt ccggtgaagg
       61 caaaatacgc gtcgacagcc agccaaccga ggaggccagt tgccacaccg ccacgatgcc
      121 caccggtaaa aaccaggggg caacgcgcaa taaccacttc ttcactggcg ttgccatcat
      181 cgtgttcctt agctttgcgc gactttacgg gggataaaat catttgccac cgcttcgcct
      241 tgcggattca gcggctgcgg ctggggaatt tccggaatgg cgacatccag atgtgggaac
      301 agcaactcgc caacccgata cgcttcttcc agatgcgggt agcccgaaag cacaaagctg
      361 tcgatgccaa gcgcggcgta ttcgttgatt cgcgcggcga ccgtaggacc atcgcccacc
      421 agcgccgttc cggcaccgcc gcgcactaag ccaacgcccg cccataaatt ggggctgatc
      481 tccagattgt cgcgcttgcc gttatgtagc gccgccattc gctgttgtcc tacggaatcc
      541 gtccgggcga atgcggcttg tgctttggcg atagtctcat catcaagatg cgagattaac
      601 cgctcggcgg cctgccacgc ttcgtcgtta gtttcacgaa caatcacatg cagacgaata
      661 ccgaaacgaa ttttgcgccc atacgcggca gctttcgccc gcacttgttc gattttctct
      721 ttaacaagtt ccggcggttc gccccaggtg aggtagagat caacttgttc tgccgccagc
      781 tcctg
//
LOCUS       DAJTYM010000300          779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTYM010000300 DAJTYM010000000
VERSION     DAJTYM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            126..614
                     /locus_tag="OO575_004156"
     CDS             126..614
                     /locus_tag="OO575_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6666872.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT          251 a          154 c          147 g          227 t
ORIGIN      
        1 ttattgccat ctaaatgtat tctgaaaacg gacatgccat tgttttctca ctgttgagta
       61 agaggccaga agcgtaatat ccaggcccag gaaaacgata acggttcaat ttaaggaata
      121 ccgaagtgtt taaatttctt gtattaacat taggcattat ctcttgccag gcttacgcag
      181 aagatacggt tatagtaaac gaccatgaca cttcagccat caaagattgt tggcaaaaaa
      241 attcagatga tgatactgac attaacgtga tcaaatcatg cctgcgacaa gaatacaatc
      301 tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta tcgttatata gaacaagtgc
      361 cacgcacagg tgcaaaaaaa cttgatactg aacaacttaa cttgcttaaa aaatcacagc
      421 gagcctggct ggattttagg gacaaagaat gtgaattaat cctttcaaat gaggatgttc
      481 aggatttaag tgacccctat tctgaatcag aatggctatc atgtatgatc atacagacca
      541 atacgcgaac tcgccagttg cagctatacc gtaactctga agatttttat ccaagccctt
      601 tgacaagagg ataattcaca tctttttggc atgttttgtt gcaagctatt cctgataaat
      661 gattgcaaca aaacatccag ccttttgact gagttattaa acatactcgc gagcgcgtaa
      721 ttttttgtct ttcagcgata attcacaatc acttcattcc gttgtacctt tagcggtgg
//
LOCUS       DAJTYM010000301          777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTYM010000301 DAJTYM010000000
VERSION     DAJTYM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..398
                     /locus_tag="OO575_004157"
     CDS             <1..398
                     /locus_tag="OO575_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6666873.1"
                     /translation="IAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYW
                     FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAK
                     SLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            422..>777
                     /locus_tag="OO575_004158"
     CDS             422..>777
                     /locus_tag="OO575_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6666874.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          203 a          174 c          223 g          177 t
ORIGIN      
        1 aaatagctta caccggagat ggtcgcgtgg tgtgggtcgg taaggatgtg acaggcatcg
       61 agccacgcaa tgccagcgtt atcgaagttc cggatattac cgctaaccga cggattaccg
      121 cgccgggtta ctggttttac cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg
      181 aagatgagcg tgatgccctt ctggctcagg tcagtgcccg gacaggggaa tgggaagaag
      241 acctgctgct ggggttaatc agcgacgaag ataaagaaaa gctgaaagcc tgtcgtattt
      301 acgcgaaatc gctgcaggcg atggatttca gcaccatcac tgataaatcc tcatacaacg
      361 ccattgaatg gcccgcctct ccggaaggtt cttcctgatt taatttatcg cgagaaaaac
      421 aatgtctgta gtgatatcag gtgcgctgac tgatggcgca ggtatcccca tgtccggata
      481 ccatattatt ctgaaatccc gggtaaacac cccggaagtg gtgatgaaca ctgttgctga
      541 tgtgatgaca ggaaacgatg gtgaatactg tttccatgcg cggactggaa aatatggtgt
      601 gtatctgaaa caggactggc gcaacgagta caacgttggc gacattgctg tatatgagga
      661 ctcaaagccc ggcacgctga atgactttct gattgctcct gatgagggcg acctgaaacc
      721 ggatgtcgtc aaacgctttg aggaaatggt ggcgcaggcg cagcagagcg ccggggc
//
LOCUS       DAJTYM010000302          757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTYM010000302 DAJTYM010000000
VERSION     DAJTYM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(58..>757)
                     /locus_tag="OO575_004159"
     CDS             complement(58..>757)
                     /locus_tag="OO575_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HCR6666875.1"
                     /translation="QPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLHAPGDI
                     QGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVCHHQPT
                     CGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQLPAQC
                     RSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLLAHGRP
                     ASDIMQAHLMVVNK"
BASE COUNT          196 a          220 c          170 g          171 t
ORIGIN      
        1 aggcgtttac gccgcatccg gcaatcaacc gcaggcggct tccaaatggc cgctttttta
       61 cttattcacc accatcaaat gtgcctgcat aatgtcactg gccgggcgac cgtgcgccag
      121 caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct gatacccggc
      181 acacaaataa ttcagccccg gtttgccgct ggcatcgagc atgaagcggt gtttcgggca
      241 gcctccccaa catgctttta acacgttaca actgcgacac tgcgccggta attgcttaaa
      301 tttatcttca ccaaacacct gctgttgcgg ggaatcgatc atttctgcaa ttgtttgctg
      361 atgcatattc cccagccgat attgcggata aacatagtga tcgcaggcgt aaacgtcgcc
      421 gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa ccgcacccgg
      481 cgcaccgaca aaattggcaa acgcccattc gatattcatc acgaaaatct tgccgacgtc
      541 gcgtttgatc cagtggtcaa aaatcgccac cagaaattca ccaaattcat cggggcgcac
      601 agaccattcc gtcagttccc cctgaatatc accaggcgca tgtagtttca gtccatcgcg
      661 gacagctgtt tcatcagcca ggcgctcgac caccggaata aactggatga attcgactcc
      721 cgcatcgcac aaaaaatcat aaacctgtaa cggttgc
//
LOCUS       DAJTYM010000303          747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTYM010000303 DAJTYM010000000
VERSION     DAJTYM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..736
                     /gene="uxaB"
                     /locus_tag="OO575_004160"
     CDS             <1..736
                     /gene="uxaB"
                     /locus_tag="OO575_004160"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6666876.1"
                     /translation="TAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKPYKERKVA
                     ILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRDELESFAS
                     AVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFALAALIAF
                     YRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWEQDLTQVP
                     GLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT          181 a          187 c          207 g          172 t
ORIGIN      
        1 caccgctgaa cacttttacc tgtttgtgat tcaggggccg aaatccttag cgactgagct
       61 gcgcctggac aaatatccgc tcaacgtgct gattgttgac gatattaaac cgtataaaga
      121 acgcaaagtg gcaatcctca acggtgcgca caccgcgctg gtgccggtgg cgttccaggc
      181 agggctggat accgtaggtg aagcgatgaa cgatgctgaa atttgcgcat tcgttgaaaa
      241 agccatctac gaagaaatta ttccggtact ggatttgcct cgtgatgaac tggaatcttt
      301 cgccagtgca gtcaccgggc gtttccgtaa cccgtacatt aagcatcagc tgctgtctat
      361 cgcgctcaac ggtatgacca agttccgcac ccgcatcctg ccgcagctgc tggcagggca
      421 gaaggcaaat ggcacacttc cggcgcgcct tactttcgca ttagcggcat tgattgcgtt
      481 ctatcgtggt gagcgtaacg gagaaacata tccggtacag gacgatgcac actggctgga
      541 acgttatcag caactctgga gccagcatcg tgaccgtgtt atcggtaccc aggaactggt
      601 agcgattgtg ttggcagaaa aagaccactg ggagcaggac ctgacgcaag tgcctggtct
      661 ggtcgagcag gttgctaatg acctggatgc gattcttgaa aaagggatgc gcgaagctgt
      721 acgtccgttg tgctaataac accgtta
//
LOCUS       DAJTYM010000304          746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTYM010000304 DAJTYM010000000
VERSION     DAJTYM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..482)
                     /locus_tag="OO575_004161"
     CDS             complement(<1..482)
                     /locus_tag="OO575_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6666877.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     "
     gene            complement(629..705)
                     /locus_tag="OO575_004162"
     tRNA            complement(629..705)
                     /locus_tag="OO575_004162"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(669..671),aa:Pro,seq:tgg)
BASE COUNT          185 a          191 c          186 g          184 t
ORIGIN      
        1 tgcaggagcg tcagggcacg catcaccagt ttatgcgtgg gtctgccgcc tttggtcacg
       61 cgatattgat tgtggatctc agccggacca tccagcgata acccaacaag aaaattattt
      121 tctgccagaa atgcacacca ttcgtcatcg agcagcacgc cgttagtctg gaaactgtta
      181 cttatcttcc tgccagcacc atatttcgcc tgtagcgcca cggcacggcg gtaaaactcc
      241 agcccgagta gcgttggttc gccgccctgc caggtaaaag ccacttcgtt ttgcagttcg
      301 ctggcggcga tatagtgacg gatatacgct tccagcgtgt catcgtccat atgcgtgact
      361 ggcttttcgc ggtaaaggga ttgtttttcg agataaaaac agtagtcaca gttcagatta
      421 caatcggaac cgctcggttt cgctatcaca tgaaaagcac gcgttggaac ctgttgcagc
      481 atggtcgctc cttgcctgaa gtcatccttc aatacgttaa gggcgaaatc ataccgtggg
      541 gcaatgagtt attacgggcg tttattgcga agggagggtg acgaaatgca cagaaaacaa
      601 aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga gaggattcga acctccgacc
      661 cactggtccc aaaccagttg cgctaccaag ctgcgctact cgccgaaata ctgctttttg
      721 aatttttagt tcaattcttt aaagtc
//
LOCUS       DAJTYM010000305          741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTYM010000305 DAJTYM010000000
VERSION     DAJTYM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>741)
                     /gene="yjeM"
                     /locus_tag="OO575_004163"
     CDS             complement(<1..>741)
                     /gene="yjeM"
                     /locus_tag="OO575_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6666878.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVMLGNALHLS"
BASE COUNT          240 a          196 c          136 g          169 t
ORIGIN      
        1 gacaaatgca gtgcgttacc cagcatcacc ccgaggctct tcatcagcac ataggtaata
       61 ttgccaaggt taacggaacc attacttaat acctgctgcc agtttgtgct gacgccccat
      121 aaaaatattg ccagcgaata accgattgaa ataacaatag cggcaaaaac aatacctttg
      181 gcaaagttct tttctggatt ttccgtttta tcgaccagac caccaaccgc ttcaattccg
      241 ccataggcaa aaatagcaaa taccacaaac gatagcattg ccagaccgga ctgataaccc
      301 gggttcggtg atgcaaggaa attaatatcc tgcgcgaaat gcccaccatt taataacaaa
      361 atagtaatgc ttactaacag caatactaaa ttcagacaca ttactgcaat accgcccacc
      421 gcagtaatgc gggcaatttt attaatcccc tttgaagcaa cgactgtgac cagaatcatc
      481 catgctactg ccagcagacc aaccacctgc gtaggctcca gtccggcaat acgccagtgc
      541 tgggtcatgt cactaccata gaggaatgtt gagaacggta cccaaacttt cgcggaggta
      601 ctcaccatcc agatgatata agaggaaaac cacataaacg taccaatgaa ggcaaaacgt
      661 ggtccgacac tattattcat ccaggaatag ataccacctt cttctttgcg ataagcagct
      721 cccatttcag ccatcattaa g
//
LOCUS       DAJTYM010000306          721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTYM010000306 DAJTYM010000000
VERSION     DAJTYM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            392..>721
                     /locus_tag="OO575_004164"
     CDS             392..>721
                     /locus_tag="OO575_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415759.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6666879.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSD"
BASE COUNT          230 a          141 c          180 g          170 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 t
//
LOCUS       DAJTYM010000307          708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTYM010000307 DAJTYM010000000
VERSION     DAJTYM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>708)
                     /locus_tag="OO575_004165"
     CDS             complement(<1..>708)
                     /locus_tag="OO575_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6666880.1"
                     /translation="GVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPG
                     FQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQ
                     ENQIAQFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLA
                     AGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLR
                     VNIREQASGAYSVSSRLS"
BASE COUNT          169 a          170 c          182 g          187 t
ORIGIN      
        1 cgagaggcga gaagaaacgc tgtatgcccc agatgcctgt tcacgaatat taacgcgtag
       61 atcttttgcc agtgcgacgt taaaagcatc gagcgccata cgcgtcgcca gattaacagg
      121 tgtccgggaa tcataacgct tccactgtga aacctgtgcc acaggttcat tttgttcttt
      181 tacagtaacc gatgcgttgt ccgtcgcgcg agttaatggt ttacctgcgg ctaatggcga
      241 atcagagtgt ttgattgagc ctaagtaacg cgtaattaac gccacgagtt tgtcttctgc
      301 gacattaccg acaatgacaa acgtgatatc cgctggagat gaaaacaact ggcgatcggc
      361 agccagcgca tctgcggcag taaactgtgc aatctgattt tcttgcagta atttcgtgcg
      421 gtcatcagca tagcgcgtct catacatcag ctgggcgaat ttctccgctg gacgctggtc
      481 gagcgttttc aacgcctgaa tttgagcatt ttgtagcgat gcccaaatat tgtcgttaat
      541 cgtgctgtgg gtgattcgct ggttaatcaa ctggaaacca ggttcagggt tattagtccg
      601 cgcgctaacg gagagcaacg tattcatgcc actgacttta ctgctcatgg ttaccgagtt
      661 ttccgcactc cagcgtttca ggctggaaga ggagagttcg ccaacgcc
//
LOCUS       DAJTYM010000308          697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTYM010000308 DAJTYM010000000
VERSION     DAJTYM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            21..281
                     /locus_tag="OO575_004166"
     CDS             21..281
                     /locus_tag="OO575_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR6666881.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            287..>697
                     /locus_tag="OO575_004167"
     CDS             287..>697
                     /locus_tag="OO575_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6666882.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQ"
BASE COUNT          200 a          159 c          178 g          160 t
ORIGIN      
        1 tttttgatcg tattgaagaa atggcatggg ctcgtcacta ccagcagatc gttcgtgaag
       61 aaaaagaaac ggaactggcg gacgacctgg aaaaaggtct gcctcagcac ctgtttgaat
      121 cgctctgcat cgaccatttg caacgccacg gtgccaataa acaggcaatc agccacgcat
      181 ttgatgatga tgttgagttt caggagcgca tggcagaaca catccggtac atggctgaaa
      241 ccattgccca ccaccaggtt gatattgatt tggaggacta aaatctatgg cgctttttca
      301 acgagcaata aacacacagg cctatctaaa agcaggaatt atggggtttg ctggcgacgg
      361 caaaacctac acagcaagcg aactggctat tggtctggta atgcttatgc ggcaacgggg
      421 aattgaagct ggtaaccgcc cggtaatgtt tctggacaca gaaacaggct ctgactgggt
      481 taaaccccgc tttgatgcgg aaaatatcga attatttacg gcaaaaaccc gctcgtttgt
      541 tgatctgctt gaagccatta atgaagcgga aagcagcggt tccgtaatga tcatcgactc
      601 catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa accgtaaacg
      661 aggactggaa ttttcagact gggcgtggct caaacag
//
LOCUS       DAJTYM010000309          692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTYM010000309 DAJTYM010000000
VERSION     DAJTYM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..499
                     /locus_tag="OO575_004168"
     CDS             <1..499
                     /locus_tag="OO575_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6666883.1"
                     /translation="QFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFI
                     LKRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRS
                     LADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKV
                     KMKKS"
     gene            complement(540..>692)
                     /locus_tag="OO575_004169"
     CDS             complement(540..>692)
                     /locus_tag="OO575_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666884.1"
                     /translation="HASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGK
                     VECPDM"
BASE COUNT          137 a          153 c          184 g          218 t
ORIGIN      
        1 gcagtttgct ttcttcacgt atattcgccc ggtgtatatg aacctggcgg gattcggcgt
       61 ggatggctta acgctggtgc tgttgagttt tggtatcgcc agctttattg gtacgtcgct
      121 ttcgtcgttc attcttaaac gttcggtaaa actggcctta gcaggcgcgc cgttaatact
      181 ggctgtgagt gcgttggtac tgacgttgtg gggaagcgat aaaatcgttg ctaccggcgt
      241 ggcgattatc tgggggctaa cttttgcatt ggttcccgtc ggctggtcaa cgtggatcac
      301 ccgctcgctg gccgatcagg cagaaaaagc cgggtctatt caggtggcgg ttattcagct
      361 tgctaatacc tgtggcgcgg caatcggcgg ttatgcgctg gataatattg gtctgacttc
      421 gccgttgatg ttgtccggca cattgatgtt gctgactgca ttgttggtta ctgcaaaggt
      481 gaaaatgaag aaatcctgat atcaggtaaa gaagcgttgg tcagtagttg ttgctgttct
      541 tacatatccg ggcattctac cttacccaac gcttcccaat aattgctctg attattacgt
      601 tccatcgcca cgatggcctc gcgtatatgt tgtttattgt tttcatctga caacattaac
      661 ttttcccacg cttcatctga taatgacgca tg
//
LOCUS       DAJTYM010000310          680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTYM010000310 DAJTYM010000000
VERSION     DAJTYM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            53..>680
                     /locus_tag="OO575_004170"
     CDS             53..>680
                     /locus_tag="OO575_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6666885.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLD"
BASE COUNT          202 a          161 c          143 g          174 t
ORIGIN      
        1 tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa ccatggatga
       61 tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa
      121 tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcacaaaa
      181 atcaacgccg acgctccttt cattaatgga tgaaggcgta cgttttacta acggctatgt
      241 ggcacacggt gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg
      301 ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt
      361 gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc
      421 aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt
      481 caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg
      541 attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg
      601 tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga
      661 tcgtgccaaa acacttgacc
//
LOCUS       DAJTYM010000311          672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTYM010000311 DAJTYM010000000
VERSION     DAJTYM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>672
                     /locus_tag="OO575_004171"
     CDS             <1..>672
                     /locus_tag="OO575_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6666886.1"
                     /translation="GVGAFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRD
                     TFNTEQVEVIKGPSGTDYGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDV
                     NQVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQH
                     NTPDGGIPTIGLPGYSAPSAGAAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHD
                     INDNT"
BASE COUNT          153 a          194 c          177 g          148 t
ORIGIN      
        1 ccggcgtggg tgcgtttttt gcgggtgaga acggtaactc caccactggc gacgccattt
       61 atatgcgtgg tgccgatacc tctaacagta tttatattga tggtattcgc gatatcggca
      121 gcgtctcgcg cgacaccttc aataccgagc aggtcgaagt gattaaaggg ccgtccggca
      181 ccgactacgg gcgcagcgca ccgacaggct cgatcaatat gatcagcaag cagccgcgca
      241 atgattccgg cattgacgcc tccgccagta ttggcagcgc ctggttccgc cgcggcacgc
      301 tggacgtcaa tcaggtcatt ggtgatacta ccgcggtgcg cctgaatgtg atgggcgaaa
      361 aaacccacga tgccggacgc gacaaagtca aaaatgagcg ttacggcgtc gccccttctg
      421 tcgcttttgg ccttggtaca gcaaatcgtt tgtatcttaa ttatctgcat gtcactcagc
      481 acaacacgcc agacggcggc attccgacca tcggtttgcc gggctattct gccccatctg
      541 cgggagcggc ggccctgaat cattccggaa aagttgatac tcataacttt tacggcacgg
      601 attccgatta cgacgattcg accaccgaca ccgccaccat gcgttttgag cacgacatca
      661 acgataatac ca
//
LOCUS       DAJTYM010000312          672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTYM010000312 DAJTYM010000000
VERSION     DAJTYM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..117)
                     /locus_tag="OO575_004172"
     CDS             complement(<1..117)
                     /locus_tag="OO575_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6666887.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGG"
     gene            complement(114..404)
                     /locus_tag="OO575_004173"
     CDS             complement(114..404)
                     /locus_tag="OO575_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6666888.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(404..>672)
                     /locus_tag="OO575_004174"
     CDS             complement(404..>672)
                     /locus_tag="OO575_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6666889.1"
                     /translation="TRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFA
                     NMDETEFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          155 a          190 c          149 g          178 t
ORIGIN      
        1 gccgccaata tacgaccagc gcccccacat gcgcagtttt ccctgaatcc agacactttc
       61 cagcgtggtg agacgaaggt gttccccgct tttgcctgta tttgttgggt aaatcataaa
      121 taaccttcct ttctccagat ttcttgtgtg cgaaaaacac cttctgcatg catcaggcgt
      181 aattcttctt tggtgtaatc gctggttttt acccgcccgt cgattaaatc gtggcatgag
      241 ctacaggcaa tcgctgcctg catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc
      301 agcctgtaat gcgccagcac agaagtttcg ggattgtgat tgcagtagcc aggaattctg
      361 acggtgcaca tctgcccccg cgccgcttta cgtaaatcca ccattacgca aactccagta
      421 actgcgcggc cacattttcg acttcctccg gagaggaaaa tttacggaac aggatccagt
      481 tccacagcac attcagtaca gatttataaa cctgctgaaa ctcggtttcg tccatgttcg
      541 caaatgcgat agattttgcc ctgcgcccac gactaccatc aggataaata tgctcggtgt
      601 aaaatccggc ctgaatggtt acccactcgc ggaaagcgtc aaacgacttt agcaatgccg
      661 tatcccgggt tc
//
LOCUS       DAJTYM010000313          669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTYM010000313 DAJTYM010000000
VERSION     DAJTYM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..282)
                     /locus_tag="OO575_004175"
     CDS             complement(<1..282)
                     /locus_tag="OO575_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical efflux pump membrane protein ArsB"
                     /protein_id="HCR6666890.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLL"
     gene            complement(336..>669)
                     /gene="arsR"
                     /locus_tag="OO575_004176"
     CDS             complement(336..>669)
                     /gene="arsR"
                     /locus_tag="OO575_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6666891.1"
                     /translation="QLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRHL
                     ALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNCS
                     GDSKNICS"
BASE COUNT          178 a          192 c          154 g          145 t
ORIGIN      
        1 cagcaagcga ccgcgtccat tgccccagcg tgaaacgtgc agcgctgccc attcaaaaaa
       61 gccggactca tccagcagca ggctgatgat aatgacggca ataaacgccg ccgtcgcgtt
      121 ccagacgata ttccacacca ccggaatatc acccggatga accacgcccg taaccaacgc
      181 cagtaccgcg ccgagcgttg cactccagcc gatgcctaaa cctttcggct gccagataac
      241 caatacgatg gtcaggacaa agatagcgcc tgccagtaac ataatgcctc ccggataaaa
      301 cacatgtgca aatccatatg tgtttagcta aatttttaac tgcaaatgtt cttactgtct
      361 ccggaacagt tttgtctggc caggttgcgg acaattgcct gaatcttttc ctgttcacat
      421 cgccaggctt catcaataat tttcgccgcc catgctggaa tatgcggcga taagcggtaa
      481 tgaacccact taccttgctt gcggtccagc aatagcccgc tttcacgcag caatgccaga
      541 tggcgggata tcttgggctg cgactgatcg agggcagtgc agagatcgca gacgcataac
      601 tctcccagtt cgctaagcag taaaacgatg cccagacggg tttcatcagc aagaattttg
      661 aacaattgg
//
LOCUS       DAJTYM010000314          654 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTYM010000314 DAJTYM010000000
VERSION     DAJTYM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..336
                     /locus_tag="OO575_004177"
     CDS             <1..336
                     /locus_tag="OO575_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666892.1"
                     /translation="ISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFNGTDG
                     GLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVS
                     DISVKLQEA"
     gene            338..>654
                     /locus_tag="OO575_004178"
     CDS             338..>654
                     /locus_tag="OO575_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6666893.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTR"
BASE COUNT          154 a          155 c          173 g          172 t
ORIGIN      
        1 atcagctggc agacactcaa acccgtcagt ctgcatgtca gtgtgacgct gaaaaaaagt
       61 gactacctgc cttcagatat tacccgacag gtacagcaat ccgtgctgga cgcatttaat
      121 ggtacagatg gtggtctgcg ggcaagagtt gcctctgttg tctctgccgg gcgctactat
      181 gccggcattt acaaaaccga tccggaacat attgatattc tgggccttac tgtgagccgt
      241 gatggttcgt catggacaac ggctgtcact ttcgggatag atgagattcc ggttctggat
      301 gtgtcggata tcagtgtgaa actccaggag gcataacgtg cagaatgtgg ctgccactgt
      361 gcttgcgcag tatgctgcca gcccccgact caatgccctc attaacagct ttaacgcagc
      421 gctttccccc gacagtttta tcagtgattt ttatggtctt atctggaaca tcgataccgc
      481 agaaaagtat ggtcttgatg tctggggaaa gattgtgggt gtcagtcgct ggctgacggt
      541 aaaggatgat tttaattacc tgggcttcag cgagtccagg atggacaccc cggtaatgga
      601 tgatccctgt ccgtttaatc aggcaccgtt ttacaacgga aaatcggata cccg
//
LOCUS       DAJTYM010000315          652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTYM010000315 DAJTYM010000000
VERSION     DAJTYM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>652
                     /gene="pdeF"
                     /locus_tag="OO575_004179"
     CDS             <1..>652
                     /gene="pdeF"
                     /locus_tag="OO575_004179"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6666894.1"
                     /translation="HINTFAHISSGEVFLFYLPLALMISMMMFFSWAALPGITLGIFV
                     RKYAELGFYETLSLTANFIIIIILCWGGYRVFTPRRNNVSHGDTRLISQRIFWQIVFP
                     ATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVV
                     RNPFYLRSYYSQLKQQVDAKVTKKEFELWLLALGALLLLLCMPLNEKSTIFSTNYTL"
BASE COUNT          150 a          136 c          141 g          225 t
ORIGIN      
        1 cacattaata ctttcgcgca tatttcttcc ggtgaggttt ttctctttta tctgcctttg
       61 gcactgatga tcagcatgat gatgtttttc agctgggcgg cattgccagg gatcacctta
      121 gggatttttg tccgcaaata tgcagagctg ggtttttacg aaacgctctc attaacggct
      181 aattttatta tcattatcat tctctgttgg ggcggttaca gggtctttac tccccggcgt
      241 aacaacgttt cacatggtga tacccgttta atttcccagc gtatattctg gcagatagtg
      301 tttcctgcaa cgctgtttct gatacttttc cagtttgctg catttgtagg attactggcg
      361 agcagagaaa atctggtcgg tgtcatgcct tttaacctcg ggactttaat caattatcag
      421 gccttactgg tgggtaatct gatcggtgtc ccgctgtgct acttcatcat tcgggtagtg
      481 cgaaatccat tttatttacg tagctattat tcgcaattaa aacagcaggt tgatgccaaa
      541 gtcaccaaaa aagagttcga gctctggcta ctggcattag gtgctttatt gttgctgtta
      601 tgcatgccgt taaatgaaaa aagcacaatt tttagcacca attatacctt gt
//
LOCUS       DAJTYM010000316          649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTYM010000316 DAJTYM010000000
VERSION     DAJTYM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..649
                     /locus_tag="OO575_004180"
                     /pseudo
     CDS             1..649
                     /locus_tag="OO575_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          245 a          118 c          115 g          171 t
ORIGIN      
        1 ctggcaatca ataacgaaga ctcctacata tatcatggga acataaatgg caatataaaa
       61 ctaacgcaca atattaattc tcaggataag aaaactaatg caaaattaat tctggatggt
      121 agtgtcaaca caaaaaatga tgttgaagtc agtaatgcca gtcttaccat gtaaggccat
      181 gcaacagagc atgcaatatt cagaagcaca gcgagtcatt gctccctggt atttctttgt
      241 ggaacggact gggtcaccgt tttgaaagaa acagagagtt catataataa aaagttcaat
      301 tctgattaca aaagtaataa tcagcagacc tcatttgatc agcctgactg gaaaaccggg
      361 gtgtttaaat ttgatacatt acacctgaac aatgctgact tttcaatatc acgcaatgcc
      421 aatgttgaag gaaatatatc agcaaataaa tcagctatca caatcggcga taaaaatgct
      481 tacattgata atcttgcagg gaaaaatatt actaataatg gttttgactt caaacaaact
      541 atcagtacta atctatccat aggagaaact aaatttacag gtggcatcac tgcacataac
      601 agccaaatag ccataggtga tcaagctgta gttacactta atggtgcaa
//
LOCUS       DAJTYM010000317          647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTYM010000317 DAJTYM010000000
VERSION     DAJTYM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            189..>647
                     /locus_tag="OO575_004181"
     CDS             189..>647
                     /locus_tag="OO575_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR6666895.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAL"
BASE COUNT          177 a          129 c          157 g          184 t
ORIGIN      
        1 aaaaaatgcg attgctaaaa caaaagtgca atttgcggca atatagttat tccatgaccg
       61 tacaatcttg cattcagtgt aaataatctt gtttagctct cgctaaacgc ggttgtataa
      121 ccgcaataca cgccgtattt tatccggcat atattgtttt tgcttgctca tggaataggg
      181 aggttgtcat gtatcaacgc tgttttgata atgccagtga aacgctgttt gtcgccggta
      241 aaacgccacg gctttcacgt tttgcattta gcgatgatcc aaaatgggag tctggacatc
      301 acgttcatga caatgaaacc gagctgattt acgtcaagaa aggggttgca aggtttacca
      361 tcgattcttc gttatatgtc gcacatgcgg atgacattgt ggtgatagaa cgcggcaggc
      421 tgcatgcggt ggcctctgac gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg
      481 gctttcagtt tcagggggct gaggaaaatc agttactgca accgcattct tgtccggtaa
      541 ttgccgcggg gcagggaaaa gaagtcataa aaaccttatt taatgagcta agtgtgattt
      601 tgccgcaaag taaaaatagc caaacatctt cgttatggga tgcatta
//
LOCUS       DAJTYM010000318          647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTYM010000318 DAJTYM010000000
VERSION     DAJTYM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            107..301
                     /locus_tag="OO575_004182"
     CDS             107..301
                     /locus_tag="OO575_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666896.1"
                     /translation="MNEIKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          200 a          150 c          135 g          162 t
ORIGIN      
        1 atctccttta gttgacgaca attccaatgc ggataaaaca cgtccaccac actgattaca
       61 tttttcatta gcacagtggt gtgatgcagc atacgaggaa aaccggatga atgagatcaa
      121 aataaaaagg ctggagcaac tgttacaggc acgccaacgg gagtttgcca ctaaatgggg
      181 acgtgccgcg ctgccacatg aacgcctaac ctattttcca cttgaagacc tcattaaaaa
      241 taatggcaaa aaaatcaggt ctgataagaa acacttctta gaatgcaatg aagacaaata
      301 acaacaatct gcatattgcc accaaccaca gatgtggcat cgttatcagc gcacttgatt
      361 ttctttatat tcactgacga gcttgccggg gcacctttgt ttgccggtca ttctggtatc
      421 atgcagatat ttacggcgta caagcccact agcatgacat tgtaataagg cctgagccta
      481 acgctttaat gtgtgtcata cactcagtgg cgtaatcgtt gacaatatgg tgcatgtaat
      541 cgtaagcgtc cagcgaaatc catctggtca taatctgaag catccgacaa agtggtgtcc
      601 accaaataag tgatctgcgc ccaatatcct gagccggaac tttgaca
//
LOCUS       DAJTYM010000319          647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTYM010000319 DAJTYM010000000
VERSION     DAJTYM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>647
                     /locus_tag="OO575_004183"
     CDS             <1..>647
                     /locus_tag="OO575_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HCR6666897.1"
                     /translation="RKALPLPELKAQAPGRAPKAGSETIIAAAFASLLGCDVQDADAD
                     FFALGGHSLLAMKLAAQLSRQFARQVTPGQVMVASTVAKLATIIDGEEDSSRRMGFET
                     ILPLREGNGPTLFCFHPASGFAWQFSVLSRYLDPQWSIIGIQSPRPHGPMQTATNLDE
                     VCEAHLATLLEQQPHGIAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEV"
BASE COUNT          133 a          171 c          202 g          141 t
ORIGIN      
        1 cgcaaagcct taccgttgcc tgaactgaag gcacaagcgc cagggcgtgc gccgaaagcg
       61 ggcagtgaaa cgattatcgc cgcggcattc gcgtcgttgc tgggttgtga cgtgcaggat
      121 gccgatgctg atttctttgc gctgggcggt cattcgctac tggcaatgaa actggcagcg
      181 cagttaagtc gacagtttgc tcgtcaggtg acgccggggc aggtgatggt cgcgtcaacc
      241 gtcgccaaac tggcaacgat tattgatggt gaagaggaca gctcccggcg catgggattc
      301 gaaaccattc tgccgttgcg tgaaggtaat ggcccgacgc tgttttgttt ccatccggcg
      361 tccggttttg cctggcagtt tagcgtgctc tcgcgttatc tcgatccaca atggtcgatt
      421 attggcattc agtcgccgcg ccctcatgga cccatgcaga cagcgacaaa cctggatgaa
      481 gtctgcgaag cgcatctggc aacgctactt gagcaacaac cgcacggcat tgcggcgcgg
      541 ctgcgtgccc gtggcgaaca ggtggcattt cttggtttgc tggatacctg gccgccagag
      601 acgcaaaact ggcaggaaaa agaggctaat ggtctggacc cggaagt
//
LOCUS       DAJTYM010000320          644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTYM010000320 DAJTYM010000000
VERSION     DAJTYM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>644
                     /gene="adrA"
                     /locus_tag="OO575_004184"
                     /gene_synonym="dgcC"
     CDS             <1..>644
                     /gene="adrA"
                     /locus_tag="OO575_004184"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6666898.1"
                     /translation="GLRFARRVRLPRAVGLAGMFLPIASTLVSHPLPGWWWLVLVGWA
                     FVWPHLAWQIASRAVDPLSREIYNLKTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLM
                     GAGGPRLFVAGLVLMVVSCLVTLELTGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSY
                     QTATKLAEHKRRLQVMSTRDGMTGVYNRRHWETMLRNEFDNCRRHNRDATLLII"
BASE COUNT          113 a          162 c          196 g          173 t
ORIGIN      
        1 ccgggctgcg cttcgcccgt cgcgtcagac taccccgtgc ggttggcctg gctggcatgt
       61 tcttaccgat tgcttcaacg ctggtttcgc acccgctgcc gggctggtgg tggctggtgt
      121 tggtcggctg ggcgttcgtc tggccgcatt tagcctggca gatagcgagc agggccgtcg
      181 atccgcttag ccgggaaatt tacaacttaa aaaccgatgc agtattagcg ggaatgtggg
      241 taggcgtaat gggcgtaaac gtgctgcctt ccaccgctat gttgatgatt ctgtgtctga
      301 atttgatggg ggcaggcggc ccccgtctgt ttgtcgcggg tctggtgttg atggtggttt
      361 cctgccttgt caccctcgag cttacgggca ttaccgtgtc gttcaatagt gctccgctgg
      421 aatggtggct ctcccttccc attattgtcg tttatcctct gatgtttggc tgggtcagct
      481 accagacggc aaccaaactg gcggaacata aacgcaggtt gcaggtcatg agtactcgcg
      541 acggcatgac gggcgtgtat aaccgacgtc attgggaaac tatgttacgc aatgaatttg
      601 ataactgtcg gcggcataat cgcgatgcaa cgttactgat tatc
//
LOCUS       DAJTYM010000321          642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTYM010000321 DAJTYM010000000
VERSION     DAJTYM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..498
                     /locus_tag="OO575_004185"
     CDS             1..498
                     /locus_tag="OO575_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6666899.1"
                     /translation="MLKKRTNGSVRYCQYTSHQCTDNKKTETDAIYDKVRSSYLLSCI
                     LKKNKNVGLILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKE
                     VGSISTFVHKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIR
                     KKNGR"
BASE COUNT          217 a          116 c          126 g          183 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc acatcagtgc
       61 actgataata aaaagacgga gacagatgcc atttacgata aagttcggag ttcttatttg
      121 ctttcatgta ttctgaaaaa aaacaaaaat gttgggctta ttcttcatgc accatcattt
      181 gtttctgtgt ctgaaaaaat cgccagaatt gtgatggcaa attactcccg taactggagt
      241 aattccgagt tggccagtgc ggttttaatg agtgaatctt ctttaaaaag aagaatgtac
      301 aaagaggtcg gttctataag tacatttgta cataaaataa agcttacaga agctatcagg
      361 aaattacgaa ggacaaatac tcccatttct gtcatatcat ctgagttagg ttacagttca
      421 ccatcgtatt tttctaaagt cttctttaaa tatttaaaga cataccctca aaacataagg
      481 aagaaaaatg gtcggtaatg tcaccaatta ggaggacttg cagagccgct tttttacgat
      541 actgagatca aacagacata atctatgcgg agatttacag cagggcatgg ttcacaaatc
      601 tgacagcgat gaattatcgg cactgcgggc agaaaatgct cg
//
LOCUS       DAJTYM010000322          642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTYM010000322 DAJTYM010000000
VERSION     DAJTYM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..333
                     /locus_tag="OO575_004186"
     CDS             <1..333
                     /locus_tag="OO575_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6666900.1"
                     /translation="GLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWS
                     TLGQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLML
                     TLQNNPPQ"
BASE COUNT          174 a          165 c          169 g          134 t
ORIGIN      
        1 ggcctgaaac gccagtatga cgatcgccta ctgctgccga tcccgcgtct gaaaaccaat
       61 aacccggaac agctggaaaa agtgctacgc cagcaaatca aaaacgtcgg tgatcgcccg
      121 ctgttgtgga gcacattggg tcagtcactg atgaagcacg gtgaatggca ggaagcatcg
      181 ctcgccttcc gcgcggcgct gaaacaacgt ccggacgcct acgattacgc atggcttgcc
      241 gacgcgctgg acagactgca caagccggaa gaagctgcag cgatgcgtcg cgacggtttg
      301 atgttaacgt tgcagaataa cccgccacag tagttccttc tcacccggag gaaagcacct
      361 ccgggacctt tctgatgcat aaaaaaacgc ctgctcttat tacggagcag gcgttaaaac
      421 aggtctgtat gacaacaagt gggtgcttca ctcaacgttg tgtccatggt gtctgatgag
      481 gcataagcga catctgtcag tggacgataa gcaccgtaaa cggctctgcg tcattccgga
      541 gtttatgagg cactaaggcg aacataagag atggaatgag catctactcg tttattatgc
      601 cacagagaat cgggaaataa catcccttaa cacttgttat ga
//
LOCUS       DAJTYM010000323          642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTYM010000323 DAJTYM010000000
VERSION     DAJTYM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            58..>642
                     /locus_tag="OO575_004187"
     CDS             58..>642
                     /locus_tag="OO575_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6666901.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          136 a          173 c          190 g          143 t
ORIGIN      
        1 gtactgcccg gagtgtggca cggttcactg gccggacgga gtcgccccgc ccttctgatg
       61 ctcccccgtt ttgccgatat ttttcagcag ggaaaccgct ggcttaactg gctggagaaa
      121 caaccggaag gggcagtccg tccggtggta attgaatcag tgacaaaaat catggcctgc
      181 gggaccacac tgatgggcta cacgcagtgg tgttgttcat caccggactg ttgccataca
      241 aaaaaggtct gcttccggtg taaaagccgc tcctgcccgc actgcggggt gaaggctggc
      301 gcacagtgga tacagtatct gctgagcctg gtcccctact gcccgtggca gcatattgtg
      361 ttcacacttc cctgccagta ctggtccctg gtgttccaca accggtggtt actggcagag
      421 atgagccgca ttgcagcgga tgtgatactg gaaatctgcc atcaggcaga tgtggagccg
      481 gggatattca cggtgatcca cacatggggg cgtgaccagc agtggcatcc gcatatccat
      541 ttatcgacaa ctgccggtgg tgtgacgtcg ggccacacct ggaaaaatct tcatttttac
      601 gcccgtaagg tgatgagcat gtggcgctac cggataacac gg
//
LOCUS       DAJTYM010000324          638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTYM010000324 DAJTYM010000000
VERSION     DAJTYM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            109..294
                     /locus_tag="OO575_004188"
     CDS             109..294
                     /locus_tag="OO575_004188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666902.1"
                     /translation="MDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
     gene            342..638
                     /locus_tag="OO575_004189"
                     /pseudo
     CDS             342..638
                     /locus_tag="OO575_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          156 a          132 c          199 g          151 t
ORIGIN      
        1 cagaccagac ggtttaaatg agccggttac gaagaagctg aggttctgcg ctttgataat
       61 tttgacgatg ccgttgagtg ttgcgcagac ctgaatattc cgttctttgt ggatgccgga
      121 aacaaaaaac tggtcttctg gtttgtacgt gttgatgacg aagggtatcc ggaaattgcc
      181 cgctgcatgg agcgggaatt tgcgacaatt cctgccggta tcaacgccga cggcatgtat
      241 tgccctgaat gcggtacggt tcactggccg gatggcgtta ttccgccttt ctgatgcttc
      301 cccgttttgc cgatattttt cagcagggaa accgatggct taactggctg gagaagcagc
      361 cggtgcagat gagccgcctg gaacattatg ccggtcagga tgaaattggc ctgcgttaca
      421 acagccaccg gacaaaacga gaagaaaacc ttgtgatgag tggcgacgag tttatggaaa
      481 ggttctcctg gcatgtggcg gataaggggt tccggatggt gatacggggt cctgagtccg
      541 gtgaagcggc gattactgga agatgtggtg tacgccataa cggagacagt gagaaaaacg
      601 gcgaggcaaa tcacaaggag agggatgtat cagcagtg
//
LOCUS       DAJTYM010000325          630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTYM010000325 DAJTYM010000000
VERSION     DAJTYM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..337
                     /locus_tag="OO575_004190"
     CDS             <1..337
                     /locus_tag="OO575_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6666903.1"
                     /translation="VLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRGI
                     DTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARVR
                     SVEEILHAL"
BASE COUNT          172 a          157 c          144 g          157 t
ORIGIN      
        1 agtgttgccc gaaaactggt ggcaacatcc agctgcatta ggtgcaactg acagtgatat
       61 cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt tgcaactgcg
      121 ccgccgaggt atcgatacaa tagtgttatg tgggatctcg accaatatcg gtgttgaatc
      181 caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag acgcctgtag
      241 tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc gcatcgcccg
      301 tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct accgcaatgg
      361 tcacatccta aatgggtgcg gttggggatc accagccttg aagagtatgc ccgccacttt
      421 aactgcgtga tacgggattt ttaaaaatca ctaaagaacg cccaagagca tgtgttttct
      481 ttagaataat caaagcgttg gattgccatg tcatatgaaa atcggcaaag ttcatatgta
      541 acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa atatttttgc cccatgcatg
      601 ccccaaaaaa tcactttccc tcactcttga
//
LOCUS       DAJTYM010000326          623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTYM010000326 DAJTYM010000000
VERSION     DAJTYM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            20..562
                     /locus_tag="OO575_004191"
     CDS             20..562
                     /locus_tag="OO575_004191"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666904.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
BASE COUNT          180 a          122 c          150 g          171 t
ORIGIN      
        1 taacaccaaa ggcgatgcca tgattggcgt caagcagcct ttaccaaaag cagttttaag
       61 aactcagcat gacaaaaata aagaagcaat aagtatcctg gattttggtg ttattgatga
      121 tggtgtgaca gataattacc aggcaataca aaatgctata gatgccgttg cttcactacc
      181 ctccggcggg gagctgttta tccctgcgag caaccaagcg gtggggtata ttgttggatc
      241 cactttgctt attcctggcg gtgttaacat cagaggagtt ggtaaggcat cgcaactccg
      301 agcaaaaagc ggacttacag gatctgtgtt aaggctgtct tatgattcag acactatcgg
      361 ccgttatctg agaaatatac gagtaactgg taataacacc tgcaatggta ttgacacaaa
      421 cattacagca gaagactctg tcatcagaca ggtttatggc tgggtatttg ataatgtaat
      481 ggtgaatgaa gttgaaaccg cattatttaa tgcaagggct ctggcactca aaatttatag
      541 catgtcaggc tggaacctgt agagtcggtc ttcacttttt aggccagtgc gtaagtgtta
      601 gtgtcagctc ctgccatttc agc
//
LOCUS       DAJTYM010000327          618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTYM010000327 DAJTYM010000000
VERSION     DAJTYM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..618)
                     /locus_tag="OO575_004192"
                     /pseudo
     CDS             complement(1..618)
                     /locus_tag="OO575_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT          187 a          116 c          124 g          191 t
ORIGIN      
        1 ttatcggaga acaggaataa atctaaaaga tggattgctg aaatacagac tccactttta
       61 catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa catcactgca
      121 aatacccaaa aaatagcaaa gtaataatga tcagtggaag acgttgattc actttctgaa
      181 ggattagccg gatattggtg ttgtgaagtg agttggttat tcaaagcgat tatagctaac
      241 tctaatccct tagctaattt ttgttgttta aatgcgggta tcatgttgct acggatgata
      301 tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg gatgcggaca
      361 gtgcgatccg actaggcaac aacgatcagt atcccatcat tacgtttggc atctcctaaa
      421 cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcatcttt ggttgtggaa
      481 acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa cttgtgcgtc
      541 agttctttct tttcttgtga gtttaagcta ctcgtaagac ccgtaacttg ttgctgtaat
      601 tgtgggacaa tagttggt
//
LOCUS       DAJTYM010000328          618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTYM010000328 DAJTYM010000000
VERSION     DAJTYM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..381)
                     /locus_tag="OO575_004193"
                     /pseudo
     CDS             complement(1..381)
                     /locus_tag="OO575_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(429..>618)
                     /locus_tag="OO575_004194"
     CDS             complement(429..>618)
                     /locus_tag="OO575_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6666905.1"
                     /translation="GIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIARYVATNAT
                     VTAGTANADSQFTVEYKK"
BASE COUNT          191 a          126 c          115 g          186 t
ORIGIN      
        1 tttaatattc agccagaaga ctgactcgcg atcatcaggc aatgacacac ctttggtgtg
       61 aataatgcgt aatatttttt cttgcgaagg ttctaatcgt gatactggcg gaataacggt
      121 aaacggcgct ttgcttttat cctctttatt aaatggatct acccaagcct ggatgagata
      181 aggtattttt tcttctttgc taaatacaga aatagattta tcagcattat tacttaagta
      241 aaccacccgc gtactaccaa tgacaacgcc tgcataagtt aatgctggtt ggcctaaaaa
      301 aacgctgagt ataatcgcgg taaataactt acattaaact gttgttttaa agagcctgat
      361 taaacaactt tttctggaca taacgactcc ctggccaaag ccaactgaat aaaaaggccc
      421 ttgataaatt atttcttata ttcgacagta aattgtgaat cagcgttcgc cgtaccagcc
      481 gttacggtcg cattggttgc cacatagcgt gcaataaact tcatttcagc cttgccttca
      541 gcatcgatag cggtataagc agagtcgtta taaagtttaa cctgcgaatt atcggactgg
      601 ttaaaaatac gaatacca
//
LOCUS       DAJTYM010000329          607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTYM010000329 DAJTYM010000000
VERSION     DAJTYM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>607
                     /gene="btsS"
                     /locus_tag="OO575_004195"
     CDS             <1..>607
                     /gene="btsS"
                     /locus_tag="OO575_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6666906.1"
                     /translation="ANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSMGGMTALSCMI
                     STIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILAIARPYEDAVR
                     LVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAASTEGILRQGFN
                     EVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPG"
BASE COUNT          127 a          151 c          180 g          149 t
ORIGIN      
        1 tgccaatacc cgtgcgatag gcgcggtaat gggcggctta ctcggcggtc cggtcgtcgg
       61 tgggctggtt ggtctgaccg gcggcttaca tcgatattcg atggggggca tgaccgcgct
      121 aagttgcatg atctcaacca tcgttgaagg attgctcggc ggcctggtac acagcatcct
      181 gatccgtcgc gggcgcactg ataaagtctt taaccccatt accgccggtg ccgtcacgtt
      241 cgtcgctgaa atggtgcaaa tgctgatcat ccttgcgatc gcccgacctt atgaagatgc
      301 ggtgcgtctg gtgagtaata ttgctgcgcc aatgatggtc accaataccg tcggcgcggc
      361 gctgtttatg cgtatattgc tcgataaacg cgcgatgttt gaaaaataca cttcggcttt
      421 ttctgccact gcgctgaaag tggcagcctc gacggaaggc attttgcgac aggggtttaa
      481 cgaagtgaac agcatgaaag tggctcaggt actgtatcag gagctggata ttggtgcagt
      541 cgcgattacc gatcgagaga aattgctggc ctttaccgga attggtgacg accaccattt
      601 acccggc
//
LOCUS       DAJTYM010000330          599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTYM010000330 DAJTYM010000000
VERSION     DAJTYM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..64)
                     /locus_tag="OO575_004196"
     CDS             complement(<1..64)
                     /locus_tag="OO575_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6666907.1"
                     /translation="MEINVTAPALLTDEHILQPFD"
     gene            complement(55..357)
                     /locus_tag="OO575_004197"
     CDS             complement(55..357)
                     /locus_tag="OO575_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6666908.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          166 a          136 c          133 g          164 t
ORIGIN      
        1 agtcaaatgg ctgaagtata tgctcatccg tcaacaatgc tggcgcggtg acatttattt
       61 ccattcaggc tttaccgcca tcagacgttc acgcccttct gcattctgca caggagcttc
      121 cagttgcgta ataaacgaat cataacgttc gttatccagc acaaataaac gttgatccag
      181 gataatgttt ttagcttcct ggtacgccag ttccatgatg aaatcggtac ggttcttatt
      241 gaccaagctc gcagcataat caataacggc tcgttcctca tcagttgccc ggatgttaat
      301 ctgatgttct cgcggtgttt tacggacgct tgcagctgta gacataaaaa cctccattgg
      361 taacaatttg ctatgcaatt aatgttatcg caaatggtaa cagaacgcta tacagaatca
      421 cttacgggaa aagcgatccc ctgataaaaa caatggcaga gacctgaaca ttgtgacgat
      481 gatctgtgtc ggcgcacaaa tcatgtctga ttacggccgt ttaatgtgat tttgataatg
      541 aaatgcctga accacggcag cctggcggct gcaaatcggg gtgtggcaga atatgagct
//
LOCUS       DAJTYM010000331          598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTYM010000331 DAJTYM010000000
VERSION     DAJTYM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..119
                     /locus_tag="OO575_004198"
     CDS             <1..119
                     /locus_tag="OO575_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6666909.1"
                     /translation="EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            177..533
                     /locus_tag="OO575_004199"
     CDS             177..533
                     /locus_tag="OO575_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666910.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          191 a          116 c          154 g          137 t
ORIGIN      
        1 gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc cggaagagcg
       61 aagctatgca gcgtgtactg gctttctatc agggtaattt tcaggaggcg atactgtgaa
      121 tgaaattagc tatcaggctt caattaccgc tggcattcgc atcaaaggag aggagcatgg
      181 aaataaaacc agaagatgag ttaagcaata tcgttttatt tccgataaaa gaggatgacc
      241 ctcgtaatca ggttaatttt ctttataagc catcggaaag accatattgc catcacgcct
      301 ctgtacgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt gcggttgtgg
      361 agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca gatgatgtaa
      421 ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg atacagattg
      481 agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa taattaaatt
      541 tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca aatcatca
//
LOCUS       DAJTYM010000332          598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTYM010000332 DAJTYM010000000
VERSION     DAJTYM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>598
                     /gene="allE"
                     /locus_tag="OO575_004200"
     CDS             <1..>598
                     /gene="allE"
                     /locus_tag="OO575_004200"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR6666911.1"
                     /translation="ANRAIVKHGNFALLTPDGLIKNIIPDFENCDATILSTPKLGASF
                     VDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFALSEGGYLYCPPGSLM
                     TFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYEGMDDVILLDFLPKEL
                     GFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT          153 a          140 c          140 g          165 t
ORIGIN      
        1 ggctaaccgt gcgattgtta aacacggtaa tttcgcactg ttaaccccgg atggtctgat
       61 aaaaaatatt attccggact ttgaaaattg tgacgcgaca atcctctcca cgccaaagct
      121 gggtgcctct tttgttgatt atctggtcac actgcatcaa aacggtggca accaacaggg
      181 cttcggtggc gaaggcattg aaacgttcct gtatgtgatc tctggaaata tcactgccaa
      241 agccgaaggc aaaacatttg ccttaagcga aggtggctat ctttattgcc caccaggctc
      301 cttaatgacg tttgttaacg cccaggccga agacagccaa atctttttat ataagcgccg
      361 ctatgttccg gtagaaggcc atgcaccgtg gctgttttct ggcaatgcca gcgaactgga
      421 acgcattcac tatgaaggta tggatgatgt gatcctgctg gattttctgc ccaaagagtt
      481 aggttttgat atgaacatgc atatcctctc ttttgcacca ggtgccagcc acggttatat
      541 cgaaacacac gttcaggaac atggtgccta tattctttcc ggtcaggggg tttataac
//
LOCUS       DAJTYM010000333          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTYM010000333 DAJTYM010000000
VERSION     DAJTYM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            3..>594
                     /gene="ssuA"
                     /locus_tag="OO575_004201"
     CDS             3..>594
                     /gene="ssuA"
                     /locus_tag="OO575_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6666912.1"
                     /translation="MKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAKSHQL
                     LEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVGVEPP
                     KPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQPTYLT
                     PADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKD"
BASE COUNT          137 a          158 c          161 g          138 t
ORIGIN      
        1 tcattaaact ggcgctggcg ggattgctta gcgtctctac ttttgcggtt gctgcagaat
       61 cctcgcctga agcgttacgt ataggctatc agaaaggcag tattggtatg gtgctggcaa
      121 aaagccacca gttactggaa aaacgctatc cgcaatcaaa aatctcctgg gtggagttcc
      181 ccgcgggtcc gcaaatgttg gaagcgttaa acgttggcag tattgatctc ggcagtaccg
      241 gggatattcc gccaatcttt gcccaggctg ccggggctga tttggtatat gtgggcgtcg
      301 agccaccgaa gcccaaagcc gaagtgattc tggtggcaga aaacagcccg atcaaaaccg
      361 tagccgatct taaaggtcac aaagttgcct ttcagaaagg ttccagttca cacaaccttt
      421 tactgcgtgc actgcgtcag gccggactta aattcaccga tatccagccc acttacctga
      481 cgcctgccga tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg
      541 atccctacta ctccgctgcg ttattacagg gcggcgtgcg ggtgctgaaa gacg
//
LOCUS       DAJTYM010000334          587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTYM010000334 DAJTYM010000000
VERSION     DAJTYM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            57..554
                     /locus_tag="OO575_004202"
                     /pseudo
     CDS             57..554
                     /locus_tag="OO575_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          142 a          144 c          181 g          120 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcgtca atacggtgtt cggtgaacgt ttacttg
//
LOCUS       DAJTYM010000335          579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTYM010000335 DAJTYM010000000
VERSION     DAJTYM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(74..289)
                     /locus_tag="OO575_004203"
     CDS             complement(74..289)
                     /locus_tag="OO575_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6666913.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          175 a          141 c          114 g          149 t
ORIGIN      
        1 attttttatt aagctcagtg atttcatttt ccaggtgatt gaatcgctga ttcatttctt
       61 catgcttcat tatttactct ccccgtgcag ccttacgctt gtcttctctt attttgaaat
      121 acagattcgt cagataagtc agaagcccca gaagcagact tcccagcaca ccaatcgcag
      181 cccactgtga tggcctgacc tgatccaacc actgcaaaaa ccagtagccg gcactgccgg
      241 cggaggtgcc gtaggcaatg cccgttgaaa ttttgtccat ggatttcata gcctcacctc
      301 cgcaaataac ggatggcgta gttttacact gagaaatgaa agggatttga aaagaaaaaa
      361 acgcaaaagc gggcgaaacg atatatacag taaggaaagc actctatcca acaaaccacc
      421 cacagttaat cggaataaaa gcagagtgct tatgaatgat cgcacgcccg aaggttagta
      481 tttctgcaca gcaattttgc aaaaaaaggc gatcattcat aacttaaacg tctttcagtc
      541 actccgggat ttcccatcat cgcagactga aagactctg
//
LOCUS       DAJTYM010000336          575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTYM010000336 DAJTYM010000000
VERSION     DAJTYM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT          204 a          115 c           88 g          168 t
ORIGIN      
        1 tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt catatcagca
       61 tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca aatgataacc
      121 tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa atacttaatg
      181 aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc cgcagaatgc
      241 gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt gttaacacta
      301 aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata aattcattat
      361 agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt tttatcaccg
      421 agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta ccgcaaaaca
      481 accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc acgtctatga
      541 caaagggcga gttatgcaaa ctgagcaaca gcgag
//
LOCUS       DAJTYM010000337          573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTYM010000337 DAJTYM010000000
VERSION     DAJTYM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            9..173
                     /locus_tag="OO575_004204"
     CDS             9..173
                     /locus_tag="OO575_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6666914.1"
                     /translation="MTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIIIKADKDVE
                     ELLETRSDLF"
     gene            178..507
                     /locus_tag="OO575_004205"
     CDS             178..507
                     /locus_tag="OO575_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6666915.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
BASE COUNT          179 a          105 c          150 g          139 t
ORIGIN      
        1 tggctgcgtt gacgtcatct tttatgctgg ttggctgtac gcctcgcatt gaagtcgctg
       61 cacctaagga accgatcact atcaatatga acgttaaaat tgagcatgag atcatcatca
      121 aggcagacaa agatgtcgaa gagctgcttg aaactcgtag cgatcttttc tgaggcgatg
      181 atgaagaaaa cattacttct ttgtgcgttt cttgttgggc tggtaagcag caatgtaatg
      241 gcattgactc tggatgaagc cagaactcag gggcgggtag gtgaaacatt ttatggttat
      301 ctggttgcgc tgaaaacgga tgctgaaaca gagaaattag taaccgaaat taatgccgaa
      361 cgtaaagcga gttaccaaca acgggcaaag caaaataatg tgtctgtaga tgatatcgcg
      421 aaactcgccg ggcaaaagct cgtagctcgg gccaaaccgg gggaatatgt acaagggata
      481 aacggtaaat gggtgcgaaa gttttaatag caaaaccgca atatcgggat aacgctacag
      541 gagaaagcga ttgatctatt ttcctgaaac aag
//
LOCUS       DAJTYM010000338          572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTYM010000338 DAJTYM010000000
VERSION     DAJTYM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..302
                     /locus_tag="OO575_004206"
     CDS             <1..302
                     /locus_tag="OO575_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR6666916.1"
                     /translation="DNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            305..>572
                     /locus_tag="OO575_004207"
     CDS             305..>572
                     /locus_tag="OO575_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6666917.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHK"
BASE COUNT          109 a          146 c          151 g          166 t
ORIGIN      
        1 tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc gcatggcaac
       61 gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca ctggcctggg
      121 gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc ggtttgccca
      181 tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc tttgaactgc
      241 gtcatttata tcaactcatc acgcgtagca attctctaac gtcgccaccg caaggagctt
      301 aatcatggct gtgctgattt ttctgggctg tctgttgggt ggtattgcta tcggtttgcc
      361 tattgcctgg gcactgttgt tgtgcggggc ggcgttaatg ttctggctgg acatgtttga
      421 tgtccagatc atggcacaaa cgctggtgaa cggagccgat agcttctccc tcctggcgat
      481 tccgttcttt gtgctggcgg gtgaaatcat gaatgcgggc ggcttgttaa aacgcattgt
      541 tgacctgccg atgaaactgg tggggcataa ac
//
LOCUS       DAJTYM010000339          566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTYM010000339 DAJTYM010000000
VERSION     DAJTYM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(83..475)
                     /gene="iraD"
                     /locus_tag="OO575_004208"
     CDS             complement(83..475)
                     /gene="iraD"
                     /locus_tag="OO575_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6666918.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(472..555)
                     /gene="idlP"
                     /locus_tag="OO575_004209"
     CDS             complement(472..555)
                     /gene="idlP"
                     /locus_tag="OO575_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6666919.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT          145 a          147 c          137 g          137 t
ORIGIN      
        1 gcctacgcat accggatgaa tacccgcatg aatccagttg ccgaacgctt ctggcgtgaa
       61 ctgcgcgagc tggagattgt gcttagctga cattctcaag cgtcgcactg cgccaaccgc
      121 cattatccca gaccagatca aacgttagcg gttcatcgtc gtggtacagc attatctcaa
      181 ggctgatcgc cagagtgtgg cagtacgctt ctttcaaatt aacctgcaac gccgcaatcc
      241 gtggctcatg aaataacagt gcagaacgca gctgacggca ataccagctc tggagatgtt
      301 tgtcgctcgg gttatgcact tgccactcgg gcatgcctga aacaagtaat gacggtaatg
      361 ccgaatgagg agaattaaaa agagtgagga gatcgctgca taccgattta cgcagcaatg
      421 acgttttatt gcctgaaatt tcaggtaaaa cagcctgaag tgattggcgc atcattttgc
      481 gcactcctga cgtttagcaa catatgcggc ttgccctgaa caccgggcac cactgtattg
      541 atgctcattt tccatagcca ttccct
//
LOCUS       DAJTYM010000340          563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTYM010000340 DAJTYM010000000
VERSION     DAJTYM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..220
                     /locus_tag="OO575_004210"
     CDS             <1..220
                     /locus_tag="OO575_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6666920.1"
                     /translation="HMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEG
                     ANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            220..563
                     /locus_tag="OO575_004211"
                     /pseudo
     CDS             220..563
                     /locus_tag="OO575_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          148 a          116 c          152 g          147 t
ORIGIN      
        1 ccatatggcg gaagtgtacc tgactcagcc gatgcagatt gtggcgggca gccaggcagg
       61 gagcaaatgg atgagtgacg acctgtacga tcgggcatca agccaggata aacgctatca
      121 cattgttgaa ggcgcaaacc atatggatct ttatgacggc aaagcctatg tggctgaagc
      181 aatttcagta ttagctccgt tctttgagga aactctgtaa tgaaacatat tcaaatccgc
      241 aatagtgata tggcctggca tattgctgcc aatattcagt ttccaccaaa ttttgatgag
      301 tcaaaacagt atccggcgat tatcagcgtc catccttttg gcagttgtaa ggagcagacg
      361 tctggcaata tctatggcaa ggcattggct gaaaaaggct atgtcgttct cgcttatgac
      421 gccagtttcc agggagaatc tggtgggtag cctcgttgga tagaagatcc gacacagcgc
      481 gtagaggata ttagccgtgt tatcgattat gcagtaactc tgccatatgt tgatgctgaa
      541 cgcattggcg tgctgggtgt gtg
//
LOCUS       DAJTYM010000341          557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTYM010000341 DAJTYM010000000
VERSION     DAJTYM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>557)
                     /locus_tag="OO575_004212"
     CDS             complement(<1..>557)
                     /locus_tag="OO575_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6666921.1"
                     /translation="LLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKG
                     GMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLM
                     TELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPE
                     ATGYGLVYFTEAMLKRHGMGFEGMS"
BASE COUNT          137 a          160 c          135 g          125 t
ORIGIN      
        1 gctcatccct tcaaaaccca taccgtggcg ttttaacatt gcttctgtga aataaaccag
       61 accgtagccg gtagcttccg ggcgaataag actaccgcca aatgaaaggc ccttaccggt
      121 gaagacgcag gcggtattgt tggagagctt tttcatcatc cccgccataa agccgacttc
      181 acgaccacca acgccgatat cacctgccgg aacgtcggta tccgcgccca ggtggcgata
      241 cagttcagtc atcagcgtct ggcaaaaacg catcacttta ccttcgcttt ttcctttcgg
      301 atcgaaatcg ctgccgcctt taccaccgcc catcggcagg gtagtcaggg catttttgaa
      361 ggtttgttca aagccgagga atttgagaat ggaaaggtta actgacggat ggaagcgcat
      421 accgcctttg tacgggccga tggcagagct gaactgcaca cgccatgcac ggttgacctg
      481 tatctggttg cgatcatcaa cccataccac gcgaaactgg atcacgcgct ccggttcaac
      541 cagacgctcc agtaatg
//
LOCUS       DAJTYM010000342          556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTYM010000342 DAJTYM010000000
VERSION     DAJTYM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            37..>556
                     /locus_tag="OO575_004213"
     CDS             37..>556
                     /locus_tag="OO575_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR6666922.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAIS"
BASE COUNT          156 a          141 c          125 g          134 t
ORIGIN      
        1 ccaaactatt atttctgagc gtaccaaata ttatttatgg ctataagcga cacaaaactg
       61 cgtactattt atggtaaacc atattcgggc ccacaagaag tggctgatgc cgatggcctc
      121 agcgtacgaa tttcaccgaa gggggtcatc cagttccagt accgctatcg ctggcatggc
      181 aagcctaatc gacttgggct tggtcgatac ccatccctgt ctttgaagga tgccagacag
      241 atcactgctg acttgcgaaa gctctatttc tcaggaacgg atccacgcac ctattttgaa
      301 gagaaggtgg agaactccat gacggtcgcc cagtgtctcg actactggtt cgacaactac
      361 gtctctacaa ctctcagaga aaagacccag gcactttatc gatcaacggt tatgaagcgc
      421 atgcatgacg ccttccctaa tcgtccggca tcttctatca cggttaagca atgggttgac
      481 ctgcttaccg aagaagaaaa agataatcca cgccgagcaa ggcaggtgct aagtcaacta
      541 agatcagcaa ttagtt
//
LOCUS       DAJTYM010000343          552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTYM010000343 DAJTYM010000000
VERSION     DAJTYM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>552
                     /locus_tag="OO575_004214"
     CDS             <1..>552
                     /locus_tag="OO575_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6666923.1"
                     /translation="NRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQA
                     KSIADELMTGCTNFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESY
                     DNGKSGEKFLQELCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNIN
                     HAAMNTLLGERVMDRMVMNGGRWV"
BASE COUNT          135 a          126 c          173 g          118 t
ORIGIN      
        1 caaccgcctg aatcgtcagt tgcgggtgga aaaaatcctg aaccgctccg gcatccagcc
       61 gttgcaccgg aagtgttcgt ttgcgaatta ccgggcgcag aacgacggtc agcgacatgc
      121 actgagtcag gcgaaatcca tagctgacga actgatgaca ggctgtacga attttgtgtt
      181 cagcggtaag cccggcaccg gaaaaaatca tcttgcagcc gcgattggca accggctgat
      241 ggcgaagggg cgtagcgtga ttatcgtcac cgtgtccgat gtcatgagcg tgttgcatga
      301 gagctacgac aacggcaaat ccggtgaaaa atttttacag gagctttgtg gtgttgacct
      361 gctggtcctg gatgaaattg gcatgcagcg ggatacgaaa aacgagcagg tggtactgaa
      421 ccagattgtt gatcgccgga cggcatcgtt acgcggtgtg gggatgctga caaatattaa
      481 ccatgcagcg atgaatacac ttctcggcga gcgggtgatg gatcgcatgg tcatgaacgg
      541 cgggcgctgg gt
//
LOCUS       DAJTYM010000344          547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTYM010000344 DAJTYM010000000
VERSION     DAJTYM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..286)
                     /locus_tag="OO575_004215"
     CDS             complement(<1..286)
                     /locus_tag="OO575_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR6666924.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINE"
BASE COUNT          171 a          115 c          111 g          150 t
ORIGIN      
        1 attcattaat ataaagatag ccacgaataa tattcaacat tcgcttttga tgggctggag
       61 tgcaaacttg cccctgatag cgttcagctg catcgctcac taaatctgcc tggcgaatga
      121 ccagctcgga tttatccagt gccagttgag caaaggttcg gagctgatta ttcacttctg
      181 attttgccca aaggaaggca atccatagcg aaagaatgat gggaaagaga actaaaaaga
      241 taatgcccgg caacgccagt aattggtgtc gtgcacgatg actcattgct atcaatttcc
      301 tgcctgttta tacccggcca ttgcaaggtg aaaccctggc taccgacgtc tttagataaa
      361 tcttacggta taaatagcac ttttgcctga tttgaaattg ataccttttt aatcgtcacc
      421 tggatgaaaa taattcattg atattcaaaa ttaattttct acaggaatac agaggcggcg
      481 ggaagatatg cccttaatgc cactgaaaaa ccacataatt tgtacagcaa taaaagtcac
      541 ggcctaa
//
LOCUS       DAJTYM010000345          543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTYM010000345 DAJTYM010000000
VERSION     DAJTYM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..543
                     /locus_tag="OO575_004216"
                     /pseudo
     CDS             1..543
                     /locus_tag="OO575_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          127 a          134 c          165 g          117 t
ORIGIN      
        1 gaactccagc gcctgctcgg caagaaaacg atggaaaatg aactcctcaa agaagccgtt
       61 gaatatggac gggcaaaaaa gtggatagcg cacgcgccct tattgcccgg ggatggggag
      121 taagcttagt cagccgttgt ctccgggtgt cgcgtgcgca gttgcacgtc attctcagac
      181 gaaccgatga ctggatggat ggccgccgca gtcgtcacac tgatgatacg gatgtgcttc
      241 tccgtataca ccatgttatc ggagagctgc ccacgtatgg ttatcgtcgg gtatgggcgc
      301 tgcttcgcag acaggcagaa cttgatggta tgcctgcgat caatgccaaa cgtgtttacc
      361 ggctcatgcg ccagaatgcg ctgttgcttg agcgaaaacc tgctgtaccg ccatcgaaac
      421 gggcacatac aggcagagtg gccgtgaaag aaagcaatca gcgatggtgc tctgacgggt
      481 tcgagttctg ctgtgataac ggagagagac tgcgtgtcac gttcgcgctg gactgctgtg
      541 atc
//
LOCUS       DAJTYM010000346          541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTYM010000346 DAJTYM010000000
VERSION     DAJTYM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..468)
                     /locus_tag="OO575_004217"
     CDS             complement(<1..468)
                     /locus_tag="OO575_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666925.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYT"
BASE COUNT          160 a          102 c           93 g          186 t
ORIGIN      
        1 tgtataaagc tcactgttaa ttgctgaagg cgtttgtatc tcatcgagaa cttgcctgcc
       61 attatggtat tctgacgcat accatttgat aatagacgcc tgcacgcgag atatttcctc
      121 ttcagcagtt ggtaaatctc tcttatctcc tgaccattcc agccttattt ctctgttctt
      181 gatatctggg ctggtctgaa aaaatgtagt aattcgctga ataacatctt cagtcattgt
      241 ggtctgacag gcaaaattat tattgctatc acagctaaca agtatacctg gcgttgtctg
      301 gtttatttta aggctgtaaa tcacttttgt attgtcttta ttgatataca gaaacaacag
      361 caccattgcc atacagatag ccataataaa aaggataaca cgaatctttt tcttcagatg
      421 aaataaaatc tcaatcagat cgatatcctg tttgatttgt tcactcataa tatatccaga
      481 aaataaaatc tgtcgcagat aatgttgtat taatagtctg tatcaggaat gttcgggtta
      541 a
//
LOCUS       DAJTYM010000347          538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTYM010000347 DAJTYM010000000
VERSION     DAJTYM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(14..268)
                     /locus_tag="OO575_004218"
     CDS             complement(14..268)
                     /locus_tag="OO575_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6666926.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            423..>538
                     /locus_tag="OO575_004219"
     CDS             423..>538
                     /locus_tag="OO575_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Stimulates the peptidyltransferase activity of the
                     70S ribosome and enhances dipeptide synthesis with
                     N-formylmethionyl-tRNA and puromycin in vitro, suggesting
                     its involvement in the formation of the first peptide bond
                     of a protein; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6666927.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATL"
BASE COUNT          134 a          131 c          143 g          130 t
ORIGIN      
        1 ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag gtcatggcct
       61 gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag actttcggtc
      121 gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg cagggcgtat
      181 tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg gcagtgcaac
      241 acgccccgca acccggacgg cattccatac gttgttctct taaggttaac caccggattg
      301 atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc acttgcccga
      361 aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac aggaattttt
      421 cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac ggcaaactgc
      481 tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct acgctgta
//
LOCUS       DAJTYM010000348          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTYM010000348 DAJTYM010000000
VERSION     DAJTYM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..139
                     /locus_tag="OO575_004220"
     CDS             <1..139
                     /locus_tag="OO575_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6666928.1"
                     /translation="AIIAALTMGFFYTLNEKRFALIIEEINQRKNKEMATEKKTASVT
                     L"
     gene            207..>533
                     /gene="ompL"
                     /locus_tag="OO575_004221"
     CDS             207..>533
                     /gene="ompL"
                     /locus_tag="OO575_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6666929.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT          165 a          101 c          117 g          150 t
ORIGIN      
        1 ggcaattatt gctgcattga cgatgggatt cttttacaca ctcaatgaaa aacggtttgc
       61 attgattatt gaggaaatca accaacgcaa aaataaagag atggcgacag aaaaaaaaac
      121 ggcttccgta acgttataaa tgtcaatagc cgctatttcc atcctggtgg gtggcggcct
      181 ccctacgttt aaaaaatgga cttattatga aaaagattaa tgcaataatt ttattatctt
      241 ctctgacctc tgcctcggta tttgctggcg catacgtcga aaacagggag gcatataatc
      301 ttgcctccga tcagggcgag gttatgctac gtgtgggtta taacttcgat atgggcgcgg
      361 gtattatgtt aaccaatacc tacacctttc agcgagaaga tgaactaaaa catggctata
      421 atgaaattga aggttggtat cctttattca aaccaaccga taaattaacc atccagcccg
      481 gtggcttaat taatgataag agtatcagtt cgggtggtgc agtgtatctg gac
//
LOCUS       DAJTYM010000349          531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTYM010000349 DAJTYM010000000
VERSION     DAJTYM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..531
                     /locus_tag="OO575_004222"
                     /pseudo
     CDS             1..531
                     /locus_tag="OO575_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          136 a          130 c          142 g          123 t
ORIGIN      
        1 ggtggtcaac aggagtgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat
       61 cgtgctctgg cacgtcagaa tgagaatcat aaggccggta ataaatacat cccttacggg
      121 aaaatggctt cctggctggt tgagtggaaa aatgccactg aaacgcaatg gcttaaagat
      181 tctccctcac agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc
      241 ttccggaagc gggctgcttt tccccgattc aaaaagcggt gacagaatga tgcattccgc
      301 tacccgcagg gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaactgggc
      361 tggatgcgct accggaatag ccgtcaggtc acgggtgttg tgaaaaatgt cactgtcagc
      421 cagtcctgcg gtacgtggta catcagtatt cagacagaaa gtgaagtatc cactccggct
      481 cacccttcag catcaatggt cgggctggat gctggcgtgg ctaaactcgc c
//
LOCUS       DAJTYM010000350          529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTYM010000350 DAJTYM010000000
VERSION     DAJTYM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..283
                     /locus_tag="OO575_004223"
     CDS             <1..283
                     /locus_tag="OO575_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6666930.1"
                     /translation="LFRRKNKLQREIQDVEKKIRDNQKRVLLLDNLSDYIKPGMSVEA
                     IQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGEMKNGEAK"
     gene            complement(384..>529)
                     /locus_tag="OO575_004224"
     CDS             complement(384..>529)
                     /locus_tag="OO575_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR6666931.1"
                     /translation="LASMEQISLVKSWLREAGAHNVLVTSAVNNNGVTELFALLHTEE
                     GCC"
BASE COUNT          160 a          128 c          110 g          131 t
ORIGIN      
        1 tctgttccgt cgtaagaaca aactgcaacg tgaaattcag gacgttgaga aaaagatccg
       61 tgacaaccag aagcgcgtcc tgttgctgga caacctgagc gattacatca agccagggat
      121 gagcgttgaa gcaatccagg gcatcatcgc cagcatgaaa ggtgactatg aagatcgcgt
      181 tgacgattac atcatcaaaa atgccgagct ctccaaagaa cgccgcgata tctccaaaaa
      241 gctgaaagct atgggcgaaa tgaaaaacgg cgaagcgaag taattcccgt tttattcaat
      301 gagggttgcc cggcaaccct cattgctcat tgattcttat ctgtgtatca ccgtcatcat
      361 tctcatctga gaaccaatcg aaattaacaa cagccttctt ctgtatgcag caaggcaaaa
      421 agttctgtaa ctccattgtt attaactgca ctggttacta acacgttgtg cgctccagct
      481 tcccgtaacc aacttttcac caaagatatt tgttccatgc tggctaaat
//
LOCUS       DAJTYM010000351          526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTYM010000351 DAJTYM010000000
VERSION     DAJTYM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>526)
                     /locus_tag="OO575_004225"
     CDS             complement(<1..>526)
                     /locus_tag="OO575_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR6666932.1"
                     /translation="AGFFVRLVVACAFAAQAPLSSADLYFNLRFLADDPQAVADLSRF
                     ENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVA
                     GMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGIN
                     AGLLNYNFSGNSVQ"
BASE COUNT          152 a          138 c          124 g          112 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagcca
//
LOCUS       DAJTYM010000352          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTYM010000352 DAJTYM010000000
VERSION     DAJTYM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>518
                     /gene="agaC"
                     /locus_tag="OO575_004226"
     CDS             <1..>518
                     /gene="agaC"
                     /locus_tag="OO575_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR6666933.1"
                     /translation="FLFRPIIVCTLTGAILGDIQTGLITGGLTELAFAGLTPAGGVQP
                     PNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILFFYSAFSLFMTKADKCAK
                     EADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALVKAMPAWLTHGFEVAGGI
                     LPAVGFGLLLRV"
BASE COUNT           94 a          131 c          139 g          154 t
ORIGIN      
        1 tatttttatt ccgcccgata atcgtttgta ccctaactgg cgctattctc ggtgatattc
       61 agactggctt aattaccggt ggtctgacag agttggcttt cgccggatta acccctgcag
      121 gtggtgttca gccgcccaac ccgattatgg cgggtctgat gaccaccgtc attgcatggt
      181 ctacgggcgt tgatgccaaa acagcaattg gtcttggcct gccgtttagt ttgttaatgc
      241 agtacgtcat tctgttcttc tattccgctt tctcattatt tatgaccaaa gccgataaat
      301 gcgcgaaaga ggcggatacg gcagcgtttt cccggcttaa ctggacaacg atgctcatcg
      361 tcgcttcagc gtatgcggtg attgctttcc tctgtactta cctggcacaa ggggcgatgc
      421 aggcgctggt gaaagcgatg cccgcctggt tgacccacgg ctttgaagtg gctggcggta
      481 ttctgcctgc cgttggtttt ggcttgctgc tgcgcgta
//
LOCUS       DAJTYM010000353          513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTYM010000353 DAJTYM010000000
VERSION     DAJTYM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..504
                     /locus_tag="OO575_004227"
     CDS             <1..504
                     /locus_tag="OO575_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6666934.1"
                     /translation="RAFAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFI
                     QSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALM
                     PEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEP
                     QDVKKAV"
BASE COUNT          173 a          102 c          106 g          132 t
ORIGIN      
        1 cgggcgtttg cagctaagtg cggactttct gatggggtta tcagaaacta cctgtctggc
       61 aagacatacc cgtctttaga tcgattagca caaatagcct acgcaacagg gcgtccaata
      121 gagtggttta tccaaagtca gagcatgcca atggttgagc ataaaaataa tcaagagaat
      181 aaaaattctc aacctgaccg cagcacattg aaaactaaac tgatcgcaat tgtcgaattg
      241 ttagaggaca aggaactgca ttctgcgatt gagttgttca gaaagaaagg atttgaagct
      301 cttatgcctg agattttttc ttccgatgat tccatccagg ctgatttgca aggtatttcg
      361 cagcaaacct tacaaacagc caaaatgctt gaatcattac caacagatac tcgtaaaaag
      421 attttgtcaa agtatggtat ttatgaacag gaaggtcttg tagctccttc acaagaacca
      481 caagatgtaa aaaaagccgt ataacaacca tga
//
LOCUS       DAJTYM010000354          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTYM010000354 DAJTYM010000000
VERSION     DAJTYM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(83..>511)
                     /locus_tag="OO575_004228"
     CDS             complement(83..>511)
                     /locus_tag="OO575_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666935.1"
                     /translation="QQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAETAPV
                     VVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTAVGF
                     YRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT          136 a          131 c          125 g          119 t
ORIGIN      
        1 ccaggggcta tataggatat gcctaatacc gtggcgtgag cagtccggaa ctggagtaga
       61 actcttagta aaaagcacta tttcatcctt gttgctgaag catggggaat aattgttcgc
      121 aaagcaaaac accgttattc attgcttcta cccgtgcctc gctttctgta ttacgaaatt
      181 gtgccaacac atgtgccagc cgataaaaac ccaccgcggt gaggtcattc gccagcaact
      241 ctgcctgatt aatagcgctc tgttcctgat agcgccagcc gttatggagc agttgaataa
      301 gtaacgcctg gcagcgcatc agcaactgat gagcagtaga cggcacaggc agaacgctgg
      361 cagaaggtag tgataccacc acaggcgcag tttctgcgtc cagcgcccag gcacgagtct
      421 ttgtcatcat tacctgtggt tccagtgtca attgcccatc aacaaaactg acaaagccag
      481 aaaccagaca cacggggtcg tctgtttgtt g
//
LOCUS       DAJTYM010000355          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTYM010000355 DAJTYM010000000
VERSION     DAJTYM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..261
                     /locus_tag="OO575_004229"
     CDS             <1..261
                     /locus_tag="OO575_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="starvation-sensing protein; maybe involved in
                     homoserine lactone degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional D-altronate/D-mannonate
                     dehydratase"
                     /protein_id="HCR6666936.1"
                     /translation="LSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPYSWRFEEG
                     YMHPGDEPGLGISFDEKLAAKYPYDPEYLPVARLEDGTLLNW"
     gene            328..>511
                     /locus_tag="OO575_004230"
     CDS             328..>511
                     /locus_tag="OO575_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6666937.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFG"
BASE COUNT          119 a          104 c          141 g          147 t
ORIGIN      
        1 ttatcgcctg tttgtcatgc ggcggcgctg cattttgacc tatgggtacc aaattttggc
       61 gttcaggagt acatgggcta ttccgaacaa atgcttgaag ttttcccgta cagctggcgc
      121 ttcgaggaag gttatatgca cccaggcgat gaaccggggc tgggtatttc tttcgatgag
      181 aaactggcgg cgaaatatcc gtatgacccg gagtatctcc ccgtcgcacg actggaagat
      241 ggcacattgt tgaactggta ataacggttc gcttaattat tttctgaata gtcatcaaat
      301 cgattaattc cgctcaagga gctaaaaatg gacgttgatg tgtcaaccag tgttgcgggt
      361 aataaaccgc aacggattcg tcgtattcag acagtgaccc tggttttatt atttatggcg
      421 gggatcgtta attttctcga ccgttcgtca ttgagcgtgg caggcgaagc gattcgtggc
      481 gagttaggat tatcggcgac agagtttggt g
//
LOCUS       DAJTYM010000356          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTYM010000356 DAJTYM010000000
VERSION     DAJTYM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.374"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT          176 a           95 c          110 g          127 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacgc acataattgg
      481 ggggggggga tgaaaatacc tgaagcag
//
LOCUS       DAJTYM010000357          504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTYM010000357 DAJTYM010000000
VERSION     DAJTYM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(164..>504)
                     /locus_tag="OO575_004231"
     CDS             complement(164..>504)
                     /locus_tag="OO575_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6666938.1"
                     /translation="IMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQ
                     LRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQNDL
                     LLRATGGVCV"
BASE COUNT          131 a          157 c          104 g          112 t
ORIGIN      
        1 accagcagcc tgataactgg cgcgtacagg cgatactgta tgcatgagtg tcatctacag
       61 caaatgaaca atgccggatg cggcgcgagc gccttgtccg gcctacaatt ttcacatatt
      121 tcatttagtt aatcgaaacc agcgtcgcat cagtcgtata cgcttacaca cacactcccc
      181 ccgtcgcccg cagcaataaa tcattctgga tctgctctga caaccgtacg ccgccgcgcg
      241 tatctaacat cacctgacac cacgcctgcg ccattggcgg agaagcatat ttcaacattt
      301 gcgcaccgca gcccagcagg aatagctgat gagtaatctc tcgccccagt tcttcagctg
      361 gcttacgcag ctgctgctgt aaacgacgaa ccgcgcgatc aaaatagcga tcctgccctt
      421 tcacttccac aaatgcttcc gacaataagt cgtatacgcc cgcttgctta ttgagaacgc
      481 gcaacacatc caggcacata atat
//
LOCUS       DAJTYM010000358          504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTYM010000358 DAJTYM010000000
VERSION     DAJTYM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>504)
                     /locus_tag="OO575_004232"
     CDS             complement(<1..>504)
                     /locus_tag="OO575_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6666939.1"
                     /translation="WKFCAPTLCNGRNVAASLPDDYVSSRVQKPTPTRLFTDIESIAR
                     LWLNVAACTSVHQKNAVRFMIITGVRPINVHNLRWDYVYEEAGEIVYPEGVIGMRGAM
                     KTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRDYVFLQPRDPMQPFSKRSLDKLVKT
                     YSPDGAV"
BASE COUNT          127 a          125 c          131 g          121 t
ORIGIN      
        1 ttacagcccc gtccgggctg tatgttttca ccagcttatc cagtgatcgt tttgaaaatg
       61 gctgcattgg atcacgtggc tgcaaaaata cataatccct gttgcactca ggaactgaat
      121 cacgccaggc tttctgctcg tcgataatcc gccggatctc aggcgttatc ggcaggcgga
      181 aagccttttg tgttttcata gcccctcgca tgccgataac cccttccgga taaacaattt
      241 caccagcctc ctcgtaaacg tagtcccagc gcaggttatg gacattaatc ggacgaacac
      301 cagtgatgat catgaagcga acagcattct tctggtgtac agaggtgcag gcagcaacat
      361 tgagccagag tcgggcgatt gattcaatat cggtaaaaag ccgtgtgggg gtaggtttct
      421 gtacgcggga ggaaacataa tcatctggca gactggcggc aacattgcgg ccgttgcaaa
      481 gagtaggtgc gcagaacttc caga
//
LOCUS       DAJTYM010000359          500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTYM010000359 DAJTYM010000000
VERSION     DAJTYM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>500
                     /locus_tag="OO575_004233"
     CDS             <1..>500
                     /locus_tag="OO575_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6666940.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAG"
BASE COUNT           99 a          139 c          117 g          145 t
ORIGIN      
        1 gtttatcctg gctgttgtta tggctatagt cctttctgtt gggctattcc gctttaataa
       61 aacataccag ataaagaccg gacaactgcg ccgctcctgg ccgatcgccg cagtgttgat
      121 cattggtttg cctttactgg cgcaatggct ttttggcgca gcactacact gggatgtccc
      181 agccctacaa ggctttaact tccgcggcgg aatggtttta attcctgaac tggcagcctt
      241 aacgctggca ctttcggttt atacctctgc atttatcgcc gagattatcc gcgctgggat
      301 ccaggcagtg ccttatggtc aacatgaagc ggctcggtca ctggggttac ccaacccggt
      361 tacgctacgc caggtcatta ttccccaggc actgcgagta attattccgc cgttaaccag
      421 ccagtatctc aacatcgtca aaaactcctc tcttgccgcc gttattggct atcccgatat
      481 ggtttcgctg tttgctggca
//
LOCUS       DAJTYM010000360          497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTYM010000360 DAJTYM010000000
VERSION     DAJTYM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT          158 a          112 c          102 g          125 t
ORIGIN      
        1 cgccatcctg acaagcgttt tatcttcaac gggaatgaga tatggaaacg tggagaatca
       61 accacagagt atacccgcgc acaccagcga acaatcagac gactgcgcag actcatcgcc
      121 aggaaacagt atacagaaaa atggttcaga aaatatgaca catggagcgc cggatattac
      181 gaactgatgg caacaaaacc attctgacgt aaacgaaatt aaccatgacg caattaaata
      241 aggcaagccg aatacatcag gaggaccatg aacatttatt tcagaatagt tatatcactg
      301 gcaattatcg catgtattta cggattaatc gttccggccc tcatatcaat gaaggatacg
      361 gtagcagtga tttctggctt tgctctggcg tgtctgaccc cgccctgcat ttatgccatt
      421 tataagggtc tttctttttc taaggataaa agatgaaaaa agttattcct gctttagccc
      481 ttgttctgct gaccacc
//
LOCUS       DAJTYM010000361          487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTYM010000361 DAJTYM010000000
VERSION     DAJTYM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>487
                     /locus_tag="OO575_004234"
     CDS             <1..>487
                     /locus_tag="OO575_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6666941.1"
                     /translation="LEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAA
                     DIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLT
                     SMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKAL
                     KR"
BASE COUNT           97 a          163 c          131 g           96 t
ORIGIN      
        1 acttgagcac ccggcggtac tcgatcttct ccagcgatat ccctcaccag aaaaactcgc
       61 ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc gtctgggtaa
      121 acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg ttcccggcac
      181 gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc tgcgtaagca
      241 aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc tttacccggt
      301 cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca ccgaggtcgc
      361 ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg cgccggtaac
      421 tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta ataaagctct
      481 caaacgg
//
LOCUS       DAJTYM010000362          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTYM010000362 DAJTYM010000000
VERSION     DAJTYM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.380"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..346
                     /locus_tag="OO575_004235"
     CDS             <1..346
                     /locus_tag="OO575_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="necessary for biosynthesis of osmoregulated
                     periplasmic glucans possibly involved in the transfer to
                     the periplasmic space; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HCR6666942.1"
                     /translation="FLEMNRQRSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIA
                     RDRHVEQALNETPEKLNRDRRLVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLN
                     PLALRKPDAASQ"
BASE COUNT          120 a          128 c          123 g          115 t
ORIGIN      
        1 gttccttgag atgaatcgcc aacgctccct tgacgatggc tttatgcacg cagtgtttaa
       61 cccgtcattt aacgctctgg caaccgcaat ggcgacagcg cgtcaccgcg ccagtaaggt
      121 gctggaaatc gcccgtgacc gccacgttga acaggcgctt aacgagacgc cagagaagct
      181 gaatcgcgat cgtcgcctgg tgctgctaag cgatccggtg acgatggccc gtctgcattt
      241 ccgcgtctgg aattcccctg aaagatattc ttcatgggtg agttattacg aagggataaa
      301 gctcaatcca ctggcattgc gtaaaccgga tgcggcttcg caataaaaac gtagttgcct
      361 gatgcgctac gcttatcagg cctacatcgt tcctgcaatt tattgatttt gcaagatttt
      421 gtaggtcgga taaggcgttc acgccacatc cggcaaaaac aacgagccaa taaaaatacc
      481 ggcgtt
//
LOCUS       DAJTYM010000363          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTYM010000363 DAJTYM010000000
VERSION     DAJTYM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.381"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>484
                     /gene="wcaD"
                     /locus_tag="OO575_004236"
     CDS             <1..>484
                     /gene="wcaD"
                     /locus_tag="OO575_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6666943.1"
                     /translation="PHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKY
                     LIYSNSYVLNFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGI
                     ILSGSFSGVMTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTR
                     L"
BASE COUNT          117 a          100 c          114 g          153 t
ORIGIN      
        1 ccacacaacc atcgtaagat attacgtttc ttttatctgg tggtcggact ggtggtggcg
       61 ctggcggcgg tggagatggc gcaaattatc ctcaccggtg gcagcagtat tatggagtcg
      121 atttcgaaat atctgattta cagcaacagc tatgtgctga atttcattaa attcggcggc
      181 aagcgcacga cagcacttta tttcgaaccg gcatttttcg ctctggcatt aatctcaatt
      241 tggctcagca tcaaacagtt tggtatcaaa acgcctaaaa cagatgccct gattctcgca
      301 gggataatat tatccggatc gttttcaggg gttatgacct ttatcctgtt ttatttgctg
      361 gagtgggcat ttcaatatct gaataaagag gcgattaaga aaaaattacc gttagcattg
      421 atttctctgg ctgtattccg ggttggtgtg gtaatcgcgt ttccttatat ttccacccgt
      481 ctgg
//
LOCUS       DAJTYM010000364          483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTYM010000364 DAJTYM010000000
VERSION     DAJTYM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.382"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..483
                     /gene="virF"
                     /locus_tag="OO575_004237"
     CDS             <1..483
                     /gene="virF"
                     /locus_tag="OO575_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6666944.1"
                     /translation="KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEA
                     LYTSISIASSLSFSDQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQ
                     ILLDIRMHHAAKLLLNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF
                     "
BASE COUNT          171 a           69 c           77 g          166 t
ORIGIN      
        1 aaaaaaatat tcctcctctc tgaggaggag gtttctatcg atttgttcaa atctataaaa
       61 gagatgcctt tcggcaaaag aaagatctat agtttagctt gccttttatc agctgtttct
      121 gatgaggaag ctttatatac ttcgatatcg atagcttctt ctcttagttt ttctgatcag
      181 ataaggaaga ttgttgaaaa aaacatcgag aagagatggc gtctttctga tatttcaaat
      241 aacttgaatt tatcagaaat agctgttaga aaacgattgg agagtgaaaa attaacattt
      301 caacaaatcc ttcttgatat tcgcatgcat catgcagcaa agcttttatt gaatagtcaa
      361 agctatatta atgatgtatc aagacttatc ggaatatcaa gcccatctta ttttataagg
      421 aaatttaatg aatattatgg tataactcca aagaaatttt acttatatca taaaaaattt
      481 taa
//
LOCUS       DAJTYM010000365          481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTYM010000365 DAJTYM010000000
VERSION     DAJTYM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.383"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..319)
                     /locus_tag="OO575_004238"
     CDS             complement(<1..319)
                     /locus_tag="OO575_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6666945.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGD"
BASE COUNT          165 a           93 c           79 g          144 t
ORIGIN      
        1 gatctccaga gataatcttt gaaaagaaca gataaccagt aatcataaaa aagaatgaca
       61 caccaacttg gcctaaattt gctaacagat ttgatgaagg tgcttcccat actccagatg
      121 acaagtatcc attccaaatt gctgcgtgat gaataagcac gaaaattgct aagaaccccc
      181 gcattccatc tatttgatta ttcctgccgt gctcaacaag atttattggg gaaattttat
      241 caaataaccc cacagctaat aacgacatca atatcacgga tgttattaat aataaactgt
      301 taatttcaat catatgcatg ctctattacg caattaaaat ttctccaagt atactgactt
      361 ttagttaatg atcatatagt tatgtgtaac tgatgaaaca aaacggagac acaccaagct
      421 ttgcactgga ttgcaaggct ttgtgctctt ctgtgaatgt gtggctatat gtttgaagat
      481 c
//
LOCUS       DAJTYM010000366          479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTYM010000366 DAJTYM010000000
VERSION     DAJTYM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.384"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..312
                     /locus_tag="OO575_004239"
     CDS             <1..312
                     /locus_tag="OO575_004239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6666946.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(388..>479)
                     /locus_tag="OO575_004240"
     CDS             complement(388..>479)
                     /locus_tag="OO575_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6666947.1"
                     /translation="GEPHKPGCVVGEPGEIKKVVVKVKADLMA"
BASE COUNT          149 a          110 c           97 g          123 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctg
//
LOCUS       DAJTYM010000367          478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTYM010000367 DAJTYM010000000
VERSION     DAJTYM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.385"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..247
                     /locus_tag="OO575_004241"
     CDS             <1..247
                     /locus_tag="OO575_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6666948.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..>478)
                     /locus_tag="OO575_004242"
     CDS             complement(294..>478)
                     /locus_tag="OO575_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6666949.1"
                     /translation="KHWAMSMAQKHVLVVDHSKFGKVRPARMGDLKRFDIVVSDCCPE
                     DEYVKYAQTHRIKLMY"
BASE COUNT          121 a          121 c          118 g          118 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgtttta
//
LOCUS       DAJTYM010000368          477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTYM010000368 DAJTYM010000000
VERSION     DAJTYM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.386"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..194
                     /gene="yehW"
                     /locus_tag="OO575_004243"
     CDS             <1..194
                     /gene="yehW"
                     /locus_tag="OO575_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6666950.1"
                     /translation="INIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGALLVALAA
                     IIADRLFERLVQALSQHAK"
     gene            complement(175..282)
                     /locus_tag="OO575_004244"
     CDS             complement(175..282)
                     /locus_tag="OO575_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6666951.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(342..>477)
                     /locus_tag="OO575_004245"
     CDS             complement(342..>477)
                     /locus_tag="OO575_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6666952.1"
                     /translation="NQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQEQGHDIFPLGI
                     "
BASE COUNT          121 a          115 c          128 g          113 t
ORIGIN      
        1 ttatcaacat tggtacggcg acgatcgcct caacggtagg ggccagtacg ctgggtacgc
       61 ccatcatcat cgggcttagc ggatttaata ccgcgtatgt gatccagggg gcgttactgg
      121 tggcactggc ggcgatcatc gcagaccgcc tgtttgaaag gctggtgcag gcgcttagcc
      181 agcacgcaaa ataaaggtat aacctgcgag catgacgcca ccaattccgc ctaacgccat
      241 aaacaggaac agggcgatga ccccaatttt agctatgcgc ataatgcact ccttatgtta
      301 acgaaaggat tgtacagtaa agcgcatttg ttaacgaatc attaaatacc gagtgggaaa
      361 atatcatggc cttgttcttg ccaactggtg agttgctgct gttgggcgga ggttcgattt
      421 tcaccgcacc acaccagcaa tgtacggcct tcgaatagtt cagggcgtag ttgattg
//
LOCUS       DAJTYM010000369          474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTYM010000369 DAJTYM010000000
VERSION     DAJTYM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.387"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..78
                     /locus_tag="OO575_004246"
     CDS             <1..78
                     /locus_tag="OO575_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6666953.1"
                     /translation="RSNNCYGQVIRRDQESALACWGIDQ"
     gene            295..>474
                     /locus_tag="OO575_004247"
     CDS             295..>474
                     /locus_tag="OO575_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666954.1"
                     /translation="MRKLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPP
                     SNSLQLLDETFSVSGT"
BASE COUNT          122 a          123 c          125 g          104 t
ORIGIN      
        1 cgttcaaata actgctatgg acaggttatt cgtcgtgacc aggaaagcgc attagcctgt
       61 tgggggatag atcaatgagc agagtcaccg cgattatctc cgctctggtt atctgcatca
      121 tcgtctgcct gtcatgggct gttaatcatt accgtgataa cgccattacc tacaaagagc
      181 agcgcgacaa aaacgccgga gaactgaagc tggcgaacgc aaccattact gacatgcagc
      241 agcgccagcg tgatgctgat gcactcgatg ctaaatacac gaaggagtta gctgatgcga
      301 aagctgaaaa tgatgctctt cggcgcaagc ttgataatgg tggtcgggtg ctcgtcaaag
      361 gaaaatgccc tgtgccatcc tcagccgaaa cctccagcgc ctccggcatg ggcaatgatg
      421 ccaccgtcga actctctcca gttgctggac gaaacgttct cggtatccgg gacg
//
LOCUS       DAJTYM010000370          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTYM010000370 DAJTYM010000000
VERSION     DAJTYM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.388"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>472
                     /locus_tag="OO575_004248"
     CDS             <1..>472
                     /locus_tag="OO575_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6666955.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAJTYM010000371          471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTYM010000371 DAJTYM010000000
VERSION     DAJTYM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.389"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            106..>471
                     /gene="treF"
                     /locus_tag="OO575_004249"
     CDS             106..>471
                     /gene="treF"
                     /locus_tag="OO575_004249"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="cytoplasmic; catalyzes the hydrolysis of trehalose
                     to glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCR6666956.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSE"
BASE COUNT          126 a          124 c          112 g          109 t
ORIGIN      
        1 ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga ctacccttaa
       61 agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct caatcagaaa
      121 attcaaaacc ctaatccaga cgaactgatg atcgaggtcg atctctgcta tgaactggac
      181 ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga gatgattgaa
      241 gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact gttcgagcat
      301 gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc acccaaaatg
      361 gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga ttttgacttg
      421 cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga g
//
LOCUS       DAJTYM010000372          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTYM010000372 DAJTYM010000000
VERSION     DAJTYM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.390"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>470
                     /locus_tag="OO575_004250"
     CDS             <1..>470
                     /locus_tag="OO575_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6666957.1"
                     /translation="AGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSG
                     IWALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKT
                     TSVVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGM"
BASE COUNT           80 a          127 c          123 g          140 t
ORIGIN      
        1 tggcgggcat tgcccctggc ataatgatgg gcgcaacgct gatgcttacc tggtggcggc
       61 aggccagtcg ccttaattta ccccgccagc aaaaagcaac gatgcaggaa atctggcact
      121 cttttgtctc cggtatctgg gcgctgtttc ttccagtcat cattattggc gggtttcgct
      181 ccggtctgtt tacccctacc gaagccgggg ccgttgctgc tttctatgcg ctgtttgtcg
      241 ccacagttat ttaccgtgaa atgaccttcg ccacactttg gcatgtactc attggcgcgg
      301 cgaaaaccac ctcagtggtt atgtttctgg tggcctcggc acaagtttcc gcctggctga
      361 ttaccattgc tgaactgccg atgatggttt ctgatttact gcaaccgttg gtcgattcac
      421 cgcgtctgct gtttatcgtc attatggtgg ctatcctgat tgtcggcatg
//
LOCUS       DAJTYM010000373          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTYM010000373 DAJTYM010000000
VERSION     DAJTYM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.391"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..467
                     /locus_tag="OO575_004251"
                     /pseudo
     CDS             1..467
                     /locus_tag="OO575_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          112 a          133 c          136 g           86 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaaat
//
LOCUS       DAJTYM010000374          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTYM010000374 DAJTYM010000000
VERSION     DAJTYM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.392"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..425
                     /gene="rlmB"
                     /locus_tag="OO575_004252"
     CDS             <1..425
                     /gene="rlmB"
                     /locus_tag="OO575_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6666958.1"
                     /translation="TDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAE
                     SVPLIRVTNLARTMRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRL
                     TREHCDELISIPMAGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT          105 a          117 c          141 g          104 t
ORIGIN      
        1 taaccgatcc gcacaacctc ggcgcgtgcc tgcgtagtgc ggacgccgca ggcgttcatg
       61 cggtgattgt gccgaaagat cgctccgcac agctcaacgc tacggcgaaa aaagtagcct
      121 gcggcgcggc agaaagcgtt ccgctgattc gggtgactaa ccttgcgcgc accatgcgta
      181 tgttgcagga agagaatatc tggatcgtcg gtacggcagg cgaggcggat catacactct
      241 atcagagcaa aatgaccgga cgcctggcgc tggtgatggg cgcggaaggt gaaggtatgc
      301 gtcgcctgac ccgtgaacat tgcgatgagt tgatcagcat cccaatggca ggaagtgttt
      361 cttccctgaa cgtttcggtt gcaaccggaa tttgcttatt tgaagcggtt cgtcagcgca
      421 gctagttcct ctgaattatg aaaagccatc cagatttgga tggtttt
//
LOCUS       DAJTYM010000375          466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTYM010000375 DAJTYM010000000
VERSION     DAJTYM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.393"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            128..>466
                     /locus_tag="OO575_004253"
     CDS             128..>466
                     /locus_tag="OO575_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR6666959.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACE"
BASE COUNT          142 a          112 c          108 g          104 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgag
//
LOCUS       DAJTYM010000376          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTYM010000376 DAJTYM010000000
VERSION     DAJTYM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.394"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>463)
                     /locus_tag="OO575_004254"
     CDS             complement(<1..>463)
                     /locus_tag="OO575_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666960.1"
                     /translation="RIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSFQAGALAG
                     GQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALREYLRQRP
                     PACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSLP"
BASE COUNT          109 a          134 c          128 g           92 t
ORIGIN      
        1 tggcagtgat aacgtcagca gattatcttc accttcgccg ctgattacct gcgcatccag
       61 cgtctggcgg ctgctatccc aaccgagcga aatacactca gcgaccggca gaataaataa
      121 gttatcgacc tgattaagag gccgtatgca ggcgggggga cgctggcgta aatattcccg
      181 caaagccaca atgcccggct ggcgtaacgg cgcgctcaac atttgccagg catcaggcga
      241 cagcggtaca acgctgctta agcggttgcg ggtagctaac agcagctcgc catcggcact
      301 gcgttttgct gcttgtgaaa caatttgccc acccgccagt gcgccagcct gaaaactaaa
      361 cagccgacgc gtagctgccg gtgagtcttc ctgttcactt cgcggccaac tgcgcgaaag
      421 gtgcaaaata ctgccggtgt cgggatcggt aaaccagatg cgt
//
LOCUS       DAJTYM010000377          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTYM010000377 DAJTYM010000000
VERSION     DAJTYM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.395"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..262)
                     /locus_tag="OO575_004255"
     CDS             complement(<1..262)
                     /locus_tag="OO575_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR6666961.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALA"
BASE COUNT          121 a           89 c          112 g          139 t
ORIGIN      
        1 gcgctagcgc aataggctca tgctgccagt ggatcatatc gtcactggtg gcatgtcccc
       61 agtgcattgg tccccagtgt tcgctcatcg ggtgatgttg ataaaacgcg tgataacgat
      121 cgttaaacca gatcaggccg tttggatcgt tcatccatcc ggcaggaggc gcgaggtgaa
      181 aatggggata gaaagtgtta ccccggtgct catgaagttt tgctagtgcg ttttgcgccg
      241 catgcaatcg agattgcgtc attttaatca tcctggttaa gcaaatttgg tgaattatta
      301 acgttaactt ttataaaaat atagtccctt actttcataa atgcgatgaa tatcgcaaat
      361 gttaacgtta actatggcgt tttgtgatcg aatatgcatg ttttagtaaa tccatgacga
      421 ttttgcgaga aagaggttta tcactatgcg taactcagat g
//
LOCUS       DAJTYM010000378          458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTYM010000378 DAJTYM010000000
VERSION     DAJTYM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.396"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>458
                     /locus_tag="OO575_004256"
     CDS             <1..>458
                     /locus_tag="OO575_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6666962.1"
                     /translation="SELGLTTLPEIPPGIKSINISKNNLSLISPLPASLTQLNVSYNR
                     LIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPELLETLDVSC
                     NGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP"
BASE COUNT          140 a          118 c           62 g          138 t
ORIGIN      
        1 tctgaattag gcctgacaac attacctgaa atccccccgg gaattaaatc aattaatata
       61 agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca acttaatgtc
      121 agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg
      181 tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc
      241 tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta
      301 tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca
      361 atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc catatgggca
      421 atcgacaata tgttaaccga tattccatac ctgccgga
//
LOCUS       DAJTYM010000379          457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTYM010000379 DAJTYM010000000
VERSION     DAJTYM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.397"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            47..358
                     /locus_tag="OO575_004257"
     CDS             47..358
                     /locus_tag="OO575_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6666963.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
BASE COUNT          139 a          109 c          101 g          108 t
ORIGIN      
        1 gagatcgact ccctacgatt tcattcagtt aatcaaagaa gtcataatgc ctggaaaaac
       61 agccgctcta tacgatgtcg ataaaacgct gaaaaatgcg cgggtcgaac tcaaaactag
      121 tcccgacgcc aaaaacaaac ttcgtgaagc tgcgcaagcc gttggggtag atttaagcgc
      181 ttttattttg agcgctgcta tggaacgcgc cgaaagtgtg cttgataacc aacgtcgccg
      241 tgagctttcg aatcaaagct gggaactgat gaaccaactc atcgctgaac ctgctcaacc
      301 gacgctcgcc ctcaaggcgt taatgaaaag gaaaaacagc gatggtcgac aagcatgaag
      361 agattactct gcccatagtc ctctcctgta attatcagtc tgatattact tatcctgggc
      421 aaaaacagtt tgattgcggt aaccctgtta tcgataa
//
LOCUS       DAJTYM010000380          456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTYM010000380 DAJTYM010000000
VERSION     DAJTYM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.398"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(133..363)
                     /locus_tag="OO575_004258"
                     /pseudo
     CDS             complement(133..363)
                     /locus_tag="OO575_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT          145 a          120 c           85 g          106 t
ORIGIN      
        1 ataagacgtt cacgtcgcat ccggcatgaa caaagcgcac tttgtcaaaa atctaaccta
       61 ctttttaatt caggaaatta ccgcaaagcc cacgccatca tacccaacgt aacaaaactc
      121 aggcacgttc cccactcgcc ccgagaaaaa tagcattaat gcgcccagcg tcagcataaa
      181 aatattgagc agaaggtaaa ttgttggcgt aaagagtaaa ttgcaaacct caggaatagc
      241 ggcaagccca gcccccagca tggcaataac cgctgcaaaa aattgcgtca gtattgggat
      301 gccgaaaaaa gtcattacca gcgaggtcaa aatccatttt ctattttgca ttattctttc
      361 cattctttct gaatggcgaa attatactcc ccgagtcccc ttgccccttc tggacacttt
      421 tccgaaatga tggcgaaaaa aacgggaccc tttggc
//
LOCUS       DAJTYM010000381          451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTYM010000381 DAJTYM010000000
VERSION     DAJTYM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.399"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(29..>451)
                     /locus_tag="OO575_004259"
     CDS             complement(29..>451)
                     /locus_tag="OO575_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6666964.1"
                     /translation="DPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAEL
                     TAVTISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEA
                     LAFAWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
BASE COUNT          114 a          125 c          118 g           94 t
ORIGIN      
        1 ataagaaaaa attcagttaa ttctgatatc acgagttggc ggggaaaata gcccccagta
       61 ccgtctcctg gcttgcgcca gtgacggaag gcagatttcc tggtaatccc gccagcgtcc
      121 gccaggcaag ccaggcgaaa gccaatgctt ccatgtcatc gccactaatg ccaacggcat
      181 cggtggtggt gacttctgtg cctagcagta ataccgccag acgcgccatg agtagcggat
      241 tacgactacc tccaccacat accatcaatc gttcgcagcc accgctcaac aaaacttgtt
      301 cagaaatggt cacggcggtg agttctgcca gtgtggcctg aacatcacgg ggatcaacac
      361 ccggaaaatg gcgtaaatgg cgctccagcc agccgtagtt aaagtattcg cgtccggtgc
      421 ttttcggtgc aggttgcgag aaatacgggt c
//
LOCUS       DAJTYM010000382          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTYM010000382 DAJTYM010000000
VERSION     DAJTYM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.400"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            101..>450
                     /locus_tag="OO575_004260"
     CDS             101..>450
                     /locus_tag="OO575_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6666965.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLA"
BASE COUNT          111 a          125 c          116 g           98 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat
//
LOCUS       DAJTYM010000383          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTYM010000383 DAJTYM010000000
VERSION     DAJTYM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.401"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..439
                     /locus_tag="OO575_004261"
                     /pseudo
     CDS             1..439
                     /locus_tag="OO575_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          110 a          121 c          105 g          114 t
ORIGIN      
        1 caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg aagagtttta
       61 ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac ccgaatggca
      121 gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga taaccgctcc
      181 tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac gtgagtttgc
      241 cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg cggatgagtc
      301 ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg acgataaacc
      361 atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt acgactggaa
      421 agtggctgat atctggacct gatatgcaaa
//
LOCUS       DAJTYM010000384          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTYM010000384 DAJTYM010000000
VERSION     DAJTYM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.402"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>442
                     /gene="ynaI"
                     /locus_tag="OO575_004262"
     CDS             <1..>442
                     /gene="ynaI"
                     /locus_tag="OO575_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6666966.1"
                     /translation="KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRIT
                     KITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREML
                     KNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQ"
BASE COUNT          118 a          102 c          101 g          121 t
ORIGIN      
        1 taaagatatt ctgagtaatt tcttttccgg gatcatgctc tatttcgacc gtcctttcag
       61 tattggcgac tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg
      121 ctggcgaatt accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct
      181 gttttcgtcg atcagcgtag aaaacccagg acgaatgacc aaccgccgca ttaccacgac
      241 cattggttta cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga
      301 gatgctgaaa aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca
      361 gtttgctgac tcgtcattga atattatggt ttattgcttt accaaaacca cggtatgggc
      421 tgagtggctt gctgcacagc aa
//
LOCUS       DAJTYM010000385          441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTYM010000385 DAJTYM010000000
VERSION     DAJTYM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.403"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..269
                     /locus_tag="OO575_004263"
     CDS             <1..269
                     /locus_tag="OO575_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6666967.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>441
                     /gene="tnpB"
                     /locus_tag="OO575_004264"
     CDS             266..>441
                     /gene="tnpB"
                     /locus_tag="OO575_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6666968.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWA"
BASE COUNT          109 a          115 c          126 g           91 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg c
//
LOCUS       DAJTYM010000386          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTYM010000386 DAJTYM010000000
VERSION     DAJTYM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.404"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..367
                     /locus_tag="OO575_004265"
     CDS             <1..367
                     /locus_tag="OO575_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6666969.1"
                     /translation="AILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGVHNHFG
                     VSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRPLPADY
                     VTHCQNGSVKIITPDSEDE"
     gene            complement(369..>440)
                     /locus_tag="OO575_004266"
     CDS             complement(369..>440)
                     /locus_tag="OO575_004266"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6666970.1"
                     /translation="YVVKVQRNQSNPTMFNKIVVFFQ"
BASE COUNT          112 a          108 c           95 g          125 t
ORIGIN      
        1 agcaatactt tgtggtggta cgtatcgtgc cagaagtgat gctttttacg atgccagtaa
       61 ctcttcgcca ttagactctc tcaatccgcg aaaaatattt atttccgcca gcggtgtgca
      121 taatcacttt ggcgtcagct ggtttaaccc tgaagatctt gccactaagc gtaaagtgat
      181 gaaccgtgga ctacggaaaa ttttgctcgc ccgccacgcg ttgttcgatg aagtggcctc
      241 tgccagcctc gcaccgatct ctgcatttga cgttctgatt agcgatcgtc cgttaccggc
      301 agattatgtt acgcactgcc agaatggttc tgtaaagatc attacacctg attcagaaga
      361 cgaatgactt actgaaaaaa caccacaatc ttgttaaaca tcgtcgggtt ggactgatta
      421 cgttgcactt tcaccacata
//
LOCUS       DAJTYM010000387          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTYM010000387 DAJTYM010000000
VERSION     DAJTYM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.405"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..440
                     /locus_tag="OO575_004267"
                     /pseudo
     CDS             1..440
                     /locus_tag="OO575_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           94 a          121 c          127 g           98 t
ORIGIN      
        1 gccggacatc agcgctatct ctgctctcac tgccgtaaaa catggcaact gcagttcact
       61 tacaccgctt ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt
      121 ggatgccggg caaccgcccg cattatgggc gttggcctca acacgatttt ccgccattta
      181 aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct
      241 gcgcggaaat ggacgaacag tggggatacg tcggggctaa atcgcgccag cgctggctgt
      301 tttacgcgta tgacaggctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta
      361 tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga
      421 cggatggctg gccgctgtat
//
LOCUS       DAJTYM010000388          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTYM010000388 DAJTYM010000000
VERSION     DAJTYM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.406"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>439)
                     /locus_tag="OO575_004268"
     CDS             complement(<1..>439)
                     /locus_tag="OO575_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6666971.1"
                     /translation="DVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYS
                     IPEDFHITIDNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKG
                     WGQAKPVERAFGVGGLEEYVDKHPALAGAYTGPNPQAKPDNYGD"
BASE COUNT          101 a          128 c           98 g          112 t
ORIGIN      
        1 ggtcgccata gttatcaggt tttgcctgcg gatttggccc cgtatatgcg ccagccagtg
       61 ccggatgctt atcaacgtat tcctcaagcc caccaacacc gaaagcacgt tcaacaggtt
      121 ttgcctggcc ccagccttta ccggcaacaa cgcttgtcca gtgcattttc gcacccatca
      181 gtaaaaacag tccttttgga tcgtcctctt ttaccttaaa gcggtagcga ttgggcgcgc
      241 ctcccgtcag ccatttattc gcagcaccac gggtgttatc aatggtgatg tgaaaatcct
      301 ccgggatact gtagcgagtc acaacatcca tgaacgagag gcgaattgaa tcaatgttct
      361 cgctcacatc gcagcgccag cccagaattt ttcgggtttt cacatcctgc cagaaccatg
      421 ttttcggacg gatcacatc
//
LOCUS       DAJTYM010000389          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTYM010000389 DAJTYM010000000
VERSION     DAJTYM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.407"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>438
                     /locus_tag="OO575_004269"
     CDS             <1..>438
                     /locus_tag="OO575_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6666972.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYET"
BASE COUNT          113 a          103 c          129 g           93 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacg
//
LOCUS       DAJTYM010000390          435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTYM010000390 DAJTYM010000000
VERSION     DAJTYM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.408"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..261
                     /locus_tag="OO575_004270"
     CDS             <1..261
                     /locus_tag="OO575_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666973.1"
                     /translation="EATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDIDGPSG
                     NVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          165 a           77 c           91 g          102 t
ORIGIN      
        1 gaagccactg ttgtaactac tgatgacatt gttcacgagg ttaatctgga aggaaagcgt
       61 attggctacg tgattaagac agaaaataaa gaaaccccat tcactgtggt tgatatcgac
      121 ggtccatcag gcaacgttaa aacacttaac gatggtgtaa aaaaaatgtg tctggtgcac
      181 ataggaaaga atctgcccgc agaaaaaaaa gccgaatttc tggcaactct gattgcaatg
      241 aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca ctgtgcaggc ctgaatgaag
      301 aacctgggac atttattcat cactcgcagt aattttaatc tgagttgagg ttaaaaaaca
      361 atgaacaccg ataaacaagt ttacccactg tattacgaag caaaaaatga caaagtaaga
      421 aaacgtctcg gtatt
//
LOCUS       DAJTYM010000391          435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTYM010000391 DAJTYM010000000
VERSION     DAJTYM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.409"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>435
                     /locus_tag="OO575_004271"
     CDS             <1..>435
                     /locus_tag="OO575_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6666974.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELL
                     NDAHPEWCFRTCCSRVDVWLNLAEYMLYLPMCDAFSSGDLKTV"
BASE COUNT          118 a           77 c          129 g          111 t
ORIGIN      
        1 ggcggtaaga cggggaatat gtttaacctg atgttgacct ctaaaaagct gacaaaaacg
       61 gcaattaacg aggcgctccg gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag
      121 gcttttttgc gggatatgat taacggtaag caaaagagct ggctggcgca ttgtactgat
      181 gcagaggcgt tatgtattga tagagtcata agtgaggtgc tggcagagca tcctggattg
      241 attagtgtcc tccggcaacg gtatgaaggg cgggggatga ctaagcgtaa aatggctgaa
      301 ttgctgaatg acgcacaccc tgagtggtgt ttccggacgt gctgcagtcg ggtagatgta
      361 tggctaaatc ttgctgaata tatgctctat ctaccgatgt gtgatgcatt ctcttccggg
      421 gatctaaaaa ccgtt
//
LOCUS       DAJTYM010000392          435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTYM010000392 DAJTYM010000000
VERSION     DAJTYM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.410"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            129..>435
                     /locus_tag="OO575_004272"
     CDS             129..>435
                     /locus_tag="OO575_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6666975.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     "
BASE COUNT          121 a           82 c          100 g          132 t
ORIGIN      
        1 tttgcgtaat taatattatt tttagataaa gccgtctctt tggtgtacta ttcctttgaa
       61 tcaaaaacac acttattaat ataagtgatg attcatggta aaggatatat tatatcaatg
      121 gagtttttat gaagcgtaat attataggcg gtgcattcac tctggcatct ctaatgctgg
      181 ccgggcatgc actggcagaa gatggtgttg ttaacttcgt cggtgaaatt gtcgacacta
      241 cttgtgaagt tacctccgat acagccgatc aaattgtccc actgggtaaa gtcagtaaaa
      301 atgcattttc aggtgtaggt agtctggcgt cgccacagaa gttcagtatt aaacttgaaa
      361 attgcccggc aacgtacact caagcagccg ttcgttttga tggtacagaa gcgcctggcg
      421 gcgacggcga cctga
//
LOCUS       DAJTYM010000393          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTYM010000393 DAJTYM010000000
VERSION     DAJTYM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.411"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            35..434
                     /locus_tag="OO575_004273"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO575_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTYM010000394          421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTYM010000394 DAJTYM010000000
VERSION     DAJTYM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.412"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..290
                     /locus_tag="OO575_004274"
                     /pseudo
     CDS             1..290
                     /locus_tag="OO575_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          138 a           79 c           92 g          112 t
ORIGIN      
        1 cagtgcttct attattattc aaaaagaact tcagccggac aatgctataa tccagggaaa
       61 ggctataagc tctgttggac ggaatgtgga aatcagcact ggtaaaaagg ggaactgaca
      121 ttaacacaag aaggattcct gcaagcaact gcttatctgc ccggaacgca gaattaaagc
      181 ataaccacac aggagccaga atactgtggt cgtacgcatt cagtattgtg acaactaata
      241 gaaataaaaa tctggccggt gcgaatatta aacgatgcga taccgtatag tgtaagtcaa
      301 aagtgacggg gtatttaaac catcttttca tatttattta acataatgca gattctgatg
      361 tattagttca gttgttcgcc agaatcagcg ctcgatcact atgcgtttta gcagtgtgct
      421 g
//
LOCUS       DAJTYM010000395          421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTYM010000395 DAJTYM010000000
VERSION     DAJTYM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.413"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            39..>421
                     /locus_tag="OO575_004275"
     CDS             39..>421
                     /locus_tag="OO575_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6666976.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPEFPGRKSSRKPLPAEL"
BASE COUNT          131 a          106 c          106 g           78 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacccga
      361 tcgccagtaa gcctgaattc ccgggacgaa aatcttcacg aaaaccgctg ccggcagaac
      421 t
//
LOCUS       DAJTYM010000396          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTYM010000396 DAJTYM010000000
VERSION     DAJTYM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.414"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            80..>418
                     /locus_tag="OO575_004276"
     CDS             80..>418
                     /locus_tag="OO575_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666977.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREF"
BASE COUNT           92 a           99 c           97 g          130 t
ORIGIN      
        1 ggtaatttgt aagtagcgtc ttgagaatgt gatcgtaatc acgtttaata gggcgacttt
       61 tccacgcctg aagataacca tgattgttcg tccacaacaa cactggctgc gccgtatttt
      121 tgtctggcac ggctcagtat tatccaagat atcctcgcgc ttactactca attttctctt
      181 ttctattgct gttatattca tgctgccctg gtacacgcat ctgggcatca aattcaccct
      241 cgcaccgttc agcattctcg gtgtcgccat cgccattttt cttggttttc gtaataatgc
      301 cgggtacgcc cgttacgttg aagcgcgaaa actttggggg cagttgatga ttgcctcacg
      361 gtcgttactg cgagaggtaa aaacgacatt gccggattcg gcaagtgtaa gggagttt
//
LOCUS       DAJTYM010000397          406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTYM010000397 DAJTYM010000000
VERSION     DAJTYM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.415"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            72..341
                     /locus_tag="OO575_004277"
     CDS             72..341
                     /locus_tag="OO575_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6666978.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            335..>406
                     /locus_tag="OO575_004278"
     CDS             335..>406
                     /locus_tag="OO575_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6666979.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHE"
BASE COUNT          118 a           90 c          110 g           88 t
ORIGIN      
        1 tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa agtagcactt
       61 agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg atcgttacta
      121 tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt accgtggaga
      181 aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg cgtcttaccg
      241 tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat cagtccacag
      301 aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga cgccacatcg
      361 tggtgagatc tggtatttta accctgatcc ggttgccggg catgaa
//
LOCUS       DAJTYM010000398          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTYM010000398 DAJTYM010000000
VERSION     DAJTYM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.416"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..249)
                     /locus_tag="OO575_004279"
     CDS             complement(<1..249)
                     /locus_tag="OO575_004279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666980.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI"
BASE COUNT          113 a           91 c           88 g          113 t
ORIGIN      
        1 aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt ttttgtttcc
       61 ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat cgtcaaaatt
      121 atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt gaagttttac
      181 gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg gtttagctga
      241 acgggccata taactacctg aaagacaatg atattgtctg tttttataac ggtaatcaca
      301 ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa cgcttattta
      361 gtaccagatc ttacttataa taaaggggct tggcagtttg gataa
//
LOCUS       DAJTYM010000399          403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTYM010000399 DAJTYM010000000
VERSION     DAJTYM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.417"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>403
                     /locus_tag="OO575_004280"
     CDS             <1..>403
                     /locus_tag="OO575_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6666981.1"
                     /translation="RGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFIS
                     LLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERL
                     WQALHDTITRNHQCRSMWQLLKKVRHFMETVS"
BASE COUNT          118 a           99 c          110 g           76 t
ORIGIN      
        1 cgcggacagc aaaaacgggt ggtcacgccg ggacagaatg aaaaatatta tctggccgga
       61 gcgctgcaca gcgggacagg taaagtcagc tatgtgggcg gcaacagcaa aagttcggcg
      121 ctgttcatca gcctgctgaa gcggcttaaa gcgacatacc gtcgtgcgaa aaccatcacg
      181 ctgatcgtgg acaactacat tatccacaaa agccgggaaa cacagagctg gctgaaggag
      241 aacccgaagt tcaggggcat ttatcagccg gtttactcgc catgggtgaa tcatgttgaa
      301 cggctatggc aggcacttca cgacacaata acgcgtaatc atcagtgccg ctcaatgtgg
      361 caactgttga aaaaagttcg ccattttatg gaaaccgtca gcc
//
LOCUS       DAJTYM010000400          402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTYM010000400 DAJTYM010000000
VERSION     DAJTYM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.418"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>402)
                     /locus_tag="OO575_004281"
     CDS             complement(<1..>402)
                     /locus_tag="OO575_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR6666982.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLP"
BASE COUNT           95 a           84 c          110 g          113 t
ORIGIN      
        1 ggtagctgtg ttgttttcag gccgtcgatc tgactgatcg cttgggcgac ggtgttttgc
       61 cattctaaag aacaggtgct gaagtcaaat gtggctgttt tattatcccg ttgcgacacg
      121 ctacaggcta agcgacgtaa ttgggtcaat ttgtctgctt gctgaacagg aactccggga
      181 aagcggcgat gacttaacgt ggtgtgtaca acttcgggcg gaaaaacgac agcaatatct
      241 ttcgccagcc aaggtaaaac agcatctgta tttttgtcag ccgcacgggt ttctgctacg
      301 aaagagggtt gttcctgaat aatggtggat aagtccgcta ccttgagaga gtccgttacc
      361 gtttgggaag gttgcaaaga attatctttc gcttgctcca ca
//
LOCUS       DAJTYM010000401          400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTYM010000401 DAJTYM010000000
VERSION     DAJTYM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.419"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            148..>400
                     /gene="nanR"
                     /locus_tag="OO575_004282"
     CDS             148..>400
                     /gene="nanR"
                     /locus_tag="OO575_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6666983.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGL"
BASE COUNT          108 a           89 c           96 g          107 t
ORIGIN      
        1 ctgcgttaca ttaatttttc gcaatagtta attattccgt taattatggt aatgatgagg
       61 cacaaagaga aaaacctgcc attttcccct actttcaatc ctgtgatagg atgtcactga
      121 tgatgttaat cacactgacc ttacagaatg ggccttatga acgcatttga ttcgcaaacc
      181 gaagattctt cacctgcaat tggtcgcaac ttgcgtagcc gcccgctggc gcgtaaaaaa
      241 ctctccgaaa tggtggaaga agagctggaa cagatgatcc gccgtcgtga atttggcgaa
      301 ggtgaacaat taccgtctga acgcgaactg atggcgttct ttaacgtcgg gcgtccttct
      361 gtgcgtgaag cgctggcagc gttaaaacgc aaaggtctgg
//
LOCUS       DAJTYM010000402          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTYM010000402 DAJTYM010000000
VERSION     DAJTYM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.420"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..325)
                     /gene="flhe"
                     /locus_tag="OO575_004283"
     CDS             complement(<1..325)
                     /gene="flhe"
                     /locus_tag="OO575_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR6666984.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEV"
BASE COUNT          103 a          123 c           92 g           80 t
ORIGIN      
        1 gcacttccca gataaatcgc aacggttctg ctgccgctat accggaaaag gccacggtgg
       61 taccgctctg cccctctaat tcgacacaac gagattgcga acacaagcga acccgcagtc
      121 ctgcaggtgt cgggccgata agctgataac gccacgccac cagcgtcatc aatcctgaag
      181 caggttgtcg cgcagaaaga ggtgcagacg acatcgactc accgcgatga tttagcgtaa
      241 ttccgacact acttgcctgc cacatcccct ccccggcggc ttgcaccagc agcggaaaca
      301 ataatatcgc taataaggtt ctcatcattt accgccaatt gtcgccgtca tgcggatatg
      361 tcggttatca gacagttcca gattcgacag gaccacta
//
LOCUS       DAJTYM010000403          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTYM010000403 DAJTYM010000000
VERSION     DAJTYM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.421"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>397)
                     /locus_tag="OO575_004284"
     CDS             complement(<1..>397)
                     /locus_tag="OO575_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6666985.1"
                     /translation="LIGFSIGGSLGLILGLISGLSRWGERLLDTSIQMLRNVPHLALI
                     PLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYGLSGIPLFIH
                     VILPDALPSIMVGVRFALGLMWLTLIVAET"
BASE COUNT          114 a          120 c           97 g           66 t
ORIGIN      
        1 ggtttcggca acaatcagcg tcagccacat caggcccaac gcaaaacgca cgccgaccat
       61 aattgagggc agggcatcgg gcaggatcac atggataaac agcggtatgc cggataatcc
      121 atagctacgc gccatctcca ccagcccgcg atcgatatta cggatgccat gccaggtgtt
      181 gatataaatg gggaacagcg taccgagcgc caccagaaag attttcgcgg attcatcaat
      241 gccaaaccac aaaatcacca gcgggatcag cgccagatgc ggcacgttgc gcaacatctg
      301 aattgaggta tccagcagcc tttctcccca gcgcgacagt ccgctaatca gtcccagaat
      361 caggcccagc gatccgccaa ttgaaaagcc aatcagc
//
LOCUS       DAJTYM010000404          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTYM010000404 DAJTYM010000000
VERSION     DAJTYM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.422"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>395)
                     /locus_tag="OO575_004285"
     CDS             complement(<1..>395)
                     /locus_tag="OO575_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6666986.1"
                     /translation="ASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQL
                     HEVKYGLSSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFS
                     AVYYSDRAVSTRIRAFIDFLSEHVKTAPGG"
BASE COUNT           96 a          104 c           92 g          103 t
ORIGIN      
        1 cctccgggag ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta
       61 cttaccgcac ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta
      121 tctgccatta actccaccaa ttctccgcga gcgatttctt tgtcgatcat gtagtcggac
      181 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
      241 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      301 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      361 ggcttcccgt agcgggaaat ataatcgggg gaggc
//
LOCUS       DAJTYM010000405          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTYM010000405 DAJTYM010000000
VERSION     DAJTYM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.423"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            1..394
                     /locus_tag="OO575_004286"
                     /pseudo
     CDS             1..394
                     /locus_tag="OO575_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          103 a           92 c          116 g           83 t
ORIGIN      
        1 tgtggcgttc atcattgatg tgtttgccgg atacatcgtg tggtgacgag tctcatcgtc
       61 tatggaaacg acattcgtgc tggatgcact ggagcaggcg ttgtgggccc gtcgtccgtc
      121 tggcaccatc catcacagcg ataaaggctc tcagtatgtg tcactggcct ataaggcgcg
      181 actaaaagaa gcaaaactgc tggcatcaac agggagtact ggtgactcgt atgacaacgc
      241 gatggcagag atcatcaagg gcctttacaa agcggaggta atacaccgta agagctggaa
      301 aaaccggaca gaagtggagc tggccacact cacgtgggtg gactggtata acaatcgacg
      361 attgctggaa aggctgggcc atatcccacc ggca
//
LOCUS       DAJTYM010000406          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTYM010000406 DAJTYM010000000
VERSION     DAJTYM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.424"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..357
                     /locus_tag="OO575_004287"
     CDS             <1..357
                     /locus_tag="OO575_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6666987.1"
                     /translation="QSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGA
                     RKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNA
                     FGAVPMFLAIQHILAA"
BASE COUNT           89 a           83 c           93 g          120 t
ORIGIN      
        1 caaagtggtg aactgattgg catctgtact tttacggcat attcgctgga aagacaacgc
       61 gtttctggcg tccttcaggg ttcacaacct tcagaaattg gtgttgtcag attagtcatg
      121 ttggggggag cgcggaagta tcaaaagcgg ggctttggtc aggacctact atgtgatttt
      181 tttgaacatg taaaaaaaat tcacccggca ttaccaatta aaggggttta tcttgatgct
      241 gaccctgccg ccattaactt ttatgctcgt ctcggctttg ttcagctttc agcgacacca
      301 aatgcttttg gtgctgtacc tatgtttttg gcgattcagc atattctcgc ggcttagatt
      361 attatcgcca cccgccaggg caagt
//
LOCUS       DAJTYM010000407          381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTYM010000407 DAJTYM010000000
VERSION     DAJTYM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.425"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..295)
                     /locus_tag="OO575_004288"
     CDS             complement(<1..295)
                     /locus_tag="OO575_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6666988.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDL"
BASE COUNT          106 a           73 c           85 g          117 t
ORIGIN      
        1 gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt ttgttaatgg
       61 ataaatccat caacttgtgg acgttttcgt cgtcccaggt ctggcagaaa gtggccaggt
      121 tctgacgagc gttgccatca agatataatt catcattgat aatctgaaat gcgacatcgt
      181 cgcgcatttc gtgcagcgga aaacgttttg attctgcgat agtggaaata gacttcgcac
      241 caaaacgtga atcgagtagt tccgacctta aatccgttac ttgcttctta tccattttaa
      301 actccttaaa atgattggat cgcattaaaa aagtaggatt tatcgataaa gtaagcaagt
      361 gcaaaggact cgtgtttaaa t
//
LOCUS       DAJTYM010000408          381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTYM010000408 DAJTYM010000000
VERSION     DAJTYM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.426"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(273..>381)
                     /locus_tag="OO575_004289"
     CDS             complement(273..>381)
                     /locus_tag="OO575_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6666989.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          106 a           87 c           77 g          111 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt t
//
LOCUS       DAJTYM010000409          380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTYM010000409 DAJTYM010000000
VERSION     DAJTYM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.427"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            40..>380
                     /locus_tag="OO575_004290"
     CDS             40..>380
                     /locus_tag="OO575_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HCR6666990.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGS"
BASE COUNT          125 a           70 c           76 g          109 t
ORIGIN      
        1 taatactttg ttcattcaat aaatgagttg agagaatata tgaataaaat atactccctt
       61 aaatatagtg ctgccactgg cggactcatt gctgtttctg aattagcgaa aagagtttct
      121 ggtaaaacaa accgaaaact tgtagcaaca atgttgtctc tggctgttgc aggtacagta
      181 aatgcagcaa atattgatat atcaaatgta tgggcgagag actatcttga tcttgcacaa
      241 aataaaggta ttttccagcc cggagcaaca gacgtaacaa tcactttaaa aaacggagat
      301 aaattctctt tccataatct ctcaattccg gatttttctg gtgcagcagc gagtggcgca
      361 gctaccgcaa taggaggttc
//
LOCUS       DAJTYM010000410          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTYM010000410 DAJTYM010000000
VERSION     DAJTYM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.428"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>378)
                     /locus_tag="OO575_004291"
     CDS             complement(<1..>378)
                     /locus_tag="OO575_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR6666991.1"
                     /translation="QLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
                     VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLT"
BASE COUNT           93 a          102 c           95 g           88 t
ORIGIN      
        1 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
       61 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
      121 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
      181 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
      241 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
      301 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
      361 actacctctg atagttga
//
LOCUS       DAJTYM010000411          376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTYM010000411 DAJTYM010000000
VERSION     DAJTYM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.429"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT          126 a           93 c           75 g           82 t
ORIGIN      
        1 ggcggaagaa aaagccagtt cttcgcccct aaataacagc atgattccag tccatgcaaa
       61 atctgatttc cagctcacac ataccgagcg ctgtacaaaa aaccgccagc caaggcagtt
      121 aagagtgtgt tcccggggtt tgcttagaat attttgtaag ttgtccgaag agatatttac
      181 aacaccagaa tgatgaatca tcagtcccct gccagaggtt cactacgtaa cctgaccgac
      241 aatgcaccat tctggtgctg aaaaaagagc actggaactg caacaaaaca acatcacaga
      301 acaggagatg gtaaggagtc agacattccc acacaatatt gtgtcagtgc aaataacaac
      361 cttcgtctca aatctc
//
LOCUS       DAJTYM010000412          376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTYM010000412 DAJTYM010000000
VERSION     DAJTYM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.430"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..137
                     /locus_tag="OO575_004292"
     CDS             <1..137
                     /locus_tag="OO575_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6666992.1"
                     /translation="VAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR
                     "
     gene            complement(191..>376)
                     /gene="azoR"
                     /locus_tag="OO575_004293"
     CDS             complement(191..>376)
                     /gene="azoR"
                     /locus_tag="OO575_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6666993.1"
                     /translation="RGGIHKDGPTDLVTPYLSTFLGFIGITDVKFVFAEGIAYGPEMA
                     AKAQSDAKAAIDSIVAA"
BASE COUNT           96 a           83 c          101 g           96 t
ORIGIN      
        1 tagtggcggc atggctgtta tgttacggac actgtaaaac agttgatgaa gcgattatct
       61 ttattcgagc cagacgctcg catattgtgc ttaaggaaga gcacaaagcg atgttgaaat
      121 tatgggaaaa caggtaagtg gattgagatg tggactgaat atctacagtc cacatcaaga
      181 ccgtgtccgg ttatgcagca acaatgctgt cgatggctgc ttttgcgtca gactgtgctt
      241 tcgctgccat ttccggaccg tatgcgatcc cttcggcgaa gacaaatttc acatcggtaa
      301 tgccgataaa gccgaggaac gtggacagat acggcgtcac caggtccgtt ggtccatctt
      361 tgtggatccc accgcg
//
LOCUS       DAJTYM010000413          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTYM010000413 DAJTYM010000000
VERSION     DAJTYM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.431"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..311)
                     /locus_tag="OO575_004294"
     CDS             complement(<1..311)
                     /locus_tag="OO575_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6666994.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTYM010000414          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTYM010000414 DAJTYM010000000
VERSION     DAJTYM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.432"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..247)
                     /gene="psiF"
                     /locus_tag="OO575_004295"
     CDS             complement(<1..247)
                     /gene="psiF"
                     /locus_tag="OO575_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6666995.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNN"
BASE COUNT           88 a           96 c           82 g          105 t
ORIGIN      
        1 gattattaca ttcacgcatc ttttgctgct gtggcgtcaa acttttttca ccaggcgcag
       61 acttgctgtt cttcaggcaa tcactcatgt aggtcttacg agcatcccct ttcaacgcct
      121 gcgccgtcgc ctgctgatta caggaggtca tacgctgttg ttgtggggtt aaggttctct
      181 cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag caaggtaacc agtaatgtta
      241 ttttcatagc accatccctc ttcatgtttt aaccatgagc gtttgcgccc gtgatctgcc
      301 attaagtctg gttgctaaca gcaaaaaaac cacccggcag cgaaaattca ctgccgggcg
      361 cagttttatt t
//
LOCUS       DAJTYM010000415          366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTYM010000415 DAJTYM010000000
VERSION     DAJTYM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.433"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..182
                     /locus_tag="OO575_004296"
     CDS             <1..182
                     /locus_tag="OO575_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6666996.1"
                     /translation="YQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRT
                     RSKKRRHFQSKVLQK"
BASE COUNT          115 a           64 c           86 g          101 t
ORIGIN      
        1 aataccagga gctggcatta ctggcgggag tgacaactaa aaactggtcc aaaacattta
       61 ctcgtcactg ggttgcaatg aaacacattt ttcaccgact ggatagtgag gctttattgt
      121 ttgtaatgag aacacgttca aaaaaaagac ggcattttca aagcaaagtg ttgcaaaagt
      181 agattgaaag gcatatattc atgcaaatct gatactttgc cgattttgta cgtgatggca
      241 aaagcaaaca aaacccgccc acaagcgggt tttttgtgcc acttatctcg gatagacatg
      301 gtgaatgcgc tggtggagga gataagggtg atttttgaat gcttgcaaca ttgatttcgt
      361 aacgtt
//
LOCUS       DAJTYM010000416          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTYM010000416 DAJTYM010000000
VERSION     DAJTYM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.434"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..213)
                     /locus_tag="OO575_004297"
     CDS             complement(<1..213)
                     /locus_tag="OO575_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6666997.1"
                     /translation="MLNVAIENQNGWNYSASAPHKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGI"
BASE COUNT           87 a          105 c           75 g           97 t
ORIGIN      
        1 aataccggtc actcgagaac ccggccagtc ttgcgactgc cctgcacatc ccaccataat
       61 ttgaatggag ctgtgcatta cgcataaaaa aaccgcttca gcgcggctat gcgctcgagt
      121 aacgttcggg tttccacgcc cggcccccgt tttatgaggt gcagatgcac tataattcca
      181 cccgttctgg ttttcaatag ctacattcaa cattttctct tacctttcat caccgaagtg
      241 aactttgttg atgcggtgcc tggtgcctcc aggtgacgtt aaccagttaa caattacagt
      301 tggctttccc acccaaacca ataaggacta acatgacttt taactgtgcc gcgtgcgcat
      361 agcc
//
LOCUS       DAJTYM010000417          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTYM010000417 DAJTYM010000000
VERSION     DAJTYM010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.435"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT           85 a           92 c           84 g          103 t
ORIGIN      
        1 tggagcatta acagataggg acacagagtc tgcttggtta tcttgcctat caatatcatt
       61 aaaaagatcg acgtaatcta tcagaggctg actgtctccc gcttcgatac gcctcataca
      121 ggcttttagc accttcaggg cctcattgat gtaacgatgc tggtcaatgc ttagcggctc
      181 actagccaga gcatcggtaa ggctctcacc aagttctccg tattggtccc ggtagatatc
      241 cctcatgtca ggctcaaaga gatctgaacg gcccatagac agttcacact cagcgatgtc
      301 cttaaggtgt tccctcattt cctcataggt agcgttgggt ctgtctgcgt gtattgcctt
      361 gagt
//
LOCUS       DAJTYM010000418          362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTYM010000418 DAJTYM010000000
VERSION     DAJTYM010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.436"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(1..362)
                     /locus_tag="OO575_004298"
                     /pseudo
     CDS             complement(1..362)
                     /locus_tag="OO575_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          127 a           84 c           82 g           69 t
ORIGIN      
        1 cttggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag atttgcaata
       61 ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt atccgaaagt
      121 ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa tgcagaaacc
      181 agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc ctgctggtaa
      241 tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc aaaacctgaa
      301 gtaatgccac gattattgag attcgttaca gtgttttgaa ataaccaacc aacaatgccg
      361 ac
//
LOCUS       DAJTYM010000419          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTYM010000419 DAJTYM010000000
VERSION     DAJTYM010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.437"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            74..>361
                     /locus_tag="OO575_004299"
     CDS             74..>361
                     /locus_tag="OO575_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6666998.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKK"
BASE COUNT           89 a           87 c           87 g           98 t
ORIGIN      
        1 catctttccg ttgcagatct tcgccctttc aaataacgta ctttacaact ttcccgaaca
       61 aggagttgtg cccgtgttgt atgttattta cgctcaagac aaagctgata gcctcgaaaa
      121 acgcctttcc gttcgtccgg cacatttagc acgtttacag ttactgcatg atgaaggtcg
      181 tttgttgaca gctggtccaa tgccagcggt agacagtaac gatccagggg ctgcgggatt
      241 tactggctca accgttattg ctgaatttga atcgctggaa gcagctcagg cctgggccga
      301 tgctgacccg tacgtagcgg caggagtcta tgaacacgtt tcagtgaaac catttaagaa
      361 a
//
LOCUS       DAJTYM010000420          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTYM010000420 DAJTYM010000000
VERSION     DAJTYM010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.438"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT          108 a           78 c           71 g          102 t
ORIGIN      
        1 gggtaaaaaa agacgggatt tgatgggatt caataagtta tgcgccgatt gcattcaaga
       61 taaaacgagt taaaaatgct caatgcttcg atatcgataa aacattcaag ataggcgcgc
      121 cgtgtatttt aattctttta agactcttca aaaatgtcat cgtagccttt aaaaacgaat
      181 tttaaaggca ccctaaaggc aggtcaatga agttaacccg ccttcgcgtt tacactctct
      241 gagagaatac tagcttcaat ctcttccaaa atctctctga cttgctcgtc gggcatatca
      301 acgagcatca acgctgccag gcgtttacgg ccctctagtt gcagtagctc ttgagtttc
//
LOCUS       DAJTYM010000421          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTYM010000421 DAJTYM010000000
VERSION     DAJTYM010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.439"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..179)
                     /locus_tag="OO575_004300"
     CDS             complement(<1..179)
                     /locus_tag="OO575_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6666999.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLG"
BASE COUNT          106 a           90 c           69 g           92 t
ORIGIN      
        1 ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca aatatacggg
       61 tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc agacgccatt
      121 gatgagaaca tcatccccac acctgacaat ccgatataaa taatacgcat catattcata
      181 gcacatcctg ataatccaac cgtacaacac ttatgctcat ttcgtcatca atccgtaatc
      241 aaaaccgctg acctggacga gttacgatga cctgacagaa gtatcagacg tcttctgcgg
      301 tttctatgat agagcaattc tgtggcggat attaagataa cgggtatctg ttcttta
//
LOCUS       DAJTYM010000422          354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTYM010000422 DAJTYM010000000
VERSION     DAJTYM010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.440"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>354)
                     /locus_tag="OO575_004301"
     CDS             complement(<1..>354)
                     /locus_tag="OO575_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6667000.1"
                     /translation="TMYVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGHCIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSL"
BASE COUNT           90 a           86 c           83 g           95 t
ORIGIN      
        1 atcagcgaat cttcggtcag tgatgccact tcgcgctcag caataaattg cccatcacga
       61 aaaacggtaa cgtcatcgca aatctcgaag atttctttca tgcggtggga gatatagaca
      121 atacagtggc cttgcgattt cagctcgcgg atgacgcgga acagggattc ggtttcggta
      181 tcggtcagcg catcggtcgg ttcatccata atgatgactt tcgactcaaa gctcagcact
      241 ttggcgattt caaccatttg ctggtcaccg atggaaagat cgcccactaa cttgtcgctt
      301 ttaaagcgca ggttaagttt agccagcaat ttatccgctt cgacatacat ggtt
//
LOCUS       DAJTYM010000423          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTYM010000423 DAJTYM010000000
VERSION     DAJTYM010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.441"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT          106 a           77 c           54 g          116 t
ORIGIN      
        1 ttcattcaat taataacaca tatattaatt gccgttaaaa ctaaaaacag catcaataat
       61 caacgcgata taataaacct gccttacata tcaactgcgc cagaggtagg attgaaaacg
      121 ctctcctgat tttccaattc attttctgga taaataaata atttattttt gtcactatta
      181 tttatgtaat catcctgtca gggagaggga tctcaattat caatgcttaa ttacgtcatc
      241 attttgatga catgaaaccg gagcattttg catggaattt tcgttttcac ccaaacgtct
      301 tgttgttgct gtcgccgccg ctcttcctct catggccagc gcagcagata ccc
//
LOCUS       DAJTYM010000424          352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTYM010000424 DAJTYM010000000
VERSION     DAJTYM010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.442"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(10..291)
                     /locus_tag="OO575_004302"
     CDS             complement(10..291)
                     /locus_tag="OO575_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR6667001.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT           95 a           95 c           92 g           70 t
ORIGIN      
        1 ttctacgtat caggtcaaaa tcattggcca ttgtggcggt gtctgattca tctcttctat
       61 catcaccgac tcgatgttgt gccagatagc ggtgatgtcc gccattgtga tgccaagcaa
      121 ccccattgcg aaccaacagg cggaaacaac gcccagaccg ctgctgatca ccgcaagccc
      181 gacataatca gaccgacgaa aacggatgtt gagtgtgctg gccagaaaca tcattacggc
      241 actaagaagt tgccagcgaa gaagaaccac gccagtggtg agggtagcca tcaaacaatt
      301 cctctgaaaa gagccgatgc cctggacagc gcggcgttgt tcacgggagg cg
//
LOCUS       DAJTYM010000425          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTYM010000425 DAJTYM010000000
VERSION     DAJTYM010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.443"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..308)
                     /gene="ynaI"
                     /locus_tag="OO575_004303"
     CDS             complement(<1..308)
                     /gene="ynaI"
                     /locus_tag="OO575_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6667002.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     "
BASE COUNT          119 a           68 c           62 g          100 t
ORIGIN      
        1 ttttcaaact gattaatcag cagaaatagc ttaatcgtca gaataaccgc aatcagggac
       61 gtacagataa aatcgataac gcctggggtg ataaatttga gtttatagtt ttctataaca
      121 taattactga tgctaccaac agcactgata attatggtgt agattaaaaa ttgcaccgca
      181 tggaataaaa atccttttct tttacgattt ccacggcgaa accaaaagct catcagaatc
      241 aatgctgcgc agctaccgaa aataatgacc agattaagcg cattatttgt aaacagttca
      301 gcgatcattg ttttatcagg ctcctccaga taattgtcgt catgccgga
//
LOCUS       DAJTYM010000426          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTYM010000426 DAJTYM010000000
VERSION     DAJTYM010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.444"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>347)
                     /locus_tag="OO575_004304"
     CDS             complement(<1..>347)
                     /locus_tag="OO575_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6667003.1"
                     /translation="GFGASARESLGIQGSEELFRNMIGYSRLMGRSEEEIKRALTALS
                     QMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAMKKGALKSADTLQKVT
                     QELNKQITAKGGW"
BASE COUNT           89 a           96 c           58 g          104 t
ORIGIN      
        1 tccagccacc tttagcggtg atttgtttat tcagttcctg agtgaccttc tgaagagtat
       61 cggcagattt taaagccccc ttcttcatag catcaaataa ttcctgttca gtctttccgg
      121 ttgctttagc gaatacctga accataccag gaacagcttc agctagttgc cccttaagct
      181 cttcagccat aatctgaccc ttaccagcca tctgtgataa tgctgtcagt gctcgcttaa
      241 tctcttcttc acttcgcccc atcagacgag aatagccaat catatttcta aaaagttctt
      301 cagaaccttg aatgcctaga gactctctag ctgaagcacc gaaacca
//
LOCUS       DAJTYM010000427          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJTYM010000427 DAJTYM010000000
VERSION     DAJTYM010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.445"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>345
                     /locus_tag="OO575_004305"
     CDS             <1..>345
                     /locus_tag="OO575_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667004.1"
                     /translation="VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFR
                     LSGGNANFIARNMASVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTA
                     YRGAITAPKATV"
BASE COUNT          104 a           77 c           77 g           87 t
ORIGIN      
        1 agttatagca caaaattcca tgttcacaac aaaaggtatt gatatctccg gtgaactgac
       61 tatgatggga atccctgaac agaatagtaa aactgtaacg ccgggtctcc actacgctgc
      121 tgatggattc aggctgagtg gtggaaatgc aaatttcatt gccagaaata tggcatctgt
      181 caccggaaat atttatgctg atgatgcagc aaccattact ctgggacagc ctgaaactga
      241 aacaccgact atatcgtctg cttatcaggc atgggcagag actcttttgt atggctttga
      301 taccgcctat cgaggcgcaa taacagcccc caaagctaca gttag
//
LOCUS       DAJTYM010000428          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJTYM010000428 DAJTYM010000000
VERSION     DAJTYM010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.446"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>343
                     /gene="arsB"
                     /locus_tag="OO575_004306"
     CDS             <1..>343
                     /gene="arsB"
                     /locus_tag="OO575_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6667005.1"
                     /translation="MYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLS
                     SIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVL
                     SQKNMTISWGYY"
BASE COUNT           70 a           86 c           96 g           91 t
ORIGIN      
        1 catgtatctg gtggtttatg gcctgcgcaa tgccggatta acggaatatc tttctggcgt
       61 actcaacgtg ctggcggata acggcctgtg ggccgcgacg ctcggcaccg gattcctcac
      121 cgccttcctc tcttctatta tgaacaatat gccgacggta ctggttggcg cgttgtccat
      181 tgatggcagc acggcatctg gcgttatcaa agaagcgatg gtttatgcca acgtgattgg
      241 ctgcgatttg gggccgaaaa ttaccccgat tggtagcctg gcaacgctgc tctggctgca
      301 cgtactttcg cagaagaata tgactatcag ctggggatat tac
//
LOCUS       DAJTYM010000429          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJTYM010000429 DAJTYM010000000
VERSION     DAJTYM010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.447"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            31..>339
                     /gene="hslJ"
                     /locus_tag="OO575_004307"
     CDS             31..>339
                     /gene="hslJ"
                     /locus_tag="OO575_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6667006.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT          104 a           72 c           88 g           75 t
ORIGIN      
        1 ctgagtcaga aactgtaatt gagaaccaca atgaagaaag tagccgcgtt tgttgcgcta
       61 agcctgctga tggcgggatg tgtaagtaat gacaaaattg ctgttacgcc agaacagcta
      121 cagcatcatc gctttgtgct ggaaagcgta aacggtaagc ccgtgaccag cgataaaaat
      181 ccgccagaaa tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg taaccgcttt
      241 agcggtgaag gcaaactgtc taatggtgaa ctgacagcca aagggctggc aatgacccgt
      301 atgatgtgcg ctaacccgca gcttaatgaa ctcgataac
//
LOCUS       DAJTYM010000430          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJTYM010000430 DAJTYM010000000
VERSION     DAJTYM010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.448"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>338)
                     /locus_tag="OO575_004308"
     CDS             complement(<1..>338)
                     /locus_tag="OO575_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6667007.1"
                     /translation="RLVGHKWFFSVPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAI
                     RLERLKDKLGNRSNASCEVEFQDAIGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMM
                     RRAFSLAIYH"
BASE COUNT           88 a          115 c           76 g           59 t
ORIGIN      
        1 catgataaat cgccagcgaa aatgcacggc gcatcatggc atggctaccc agggcgcaat
       61 caaaacgcgt catccccccc attttcagga tcagacgaat tccttcccct tcctgcccca
      121 ataaccagcc aatggcatcc tgaaactcca cttcgcaact ggcgttagag cgattaccca
      181 gcttatcttt cagccgctcg aggcgaatcg cgttgcgttg cccgtcaggc aaaaagcgcg
      241 gcacaaaaaa gcaggacaga ccgcccgcgg tctgcgccag caccagatgc gcatcgcttt
      301 gcggcaccga gaaaaaccat ttatgcccca ccagccga
//
LOCUS       DAJTYM010000431          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJTYM010000431 DAJTYM010000000
VERSION     DAJTYM010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.449"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>338)
                     /locus_tag="OO575_004309"
     CDS             complement(<1..>338)
                     /locus_tag="OO575_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667008.1"
                     /translation="LWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNY
                     GNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSAD
                     NVDGVYYRPL"
BASE COUNT           85 a           95 c           74 g           84 t
ORIGIN      
        1 gcagtggtcg gtaatacaca ccgtcaacgt tatccgctga gttacggccg gtatccgata
       61 tcgccatccc tgacagcccg tgtcccgaag gtgcgcaaaa tctccacgaa acggtgttac
      121 tgccggggct gtaatacatt tcatggccat accgtacatc ctgtaccccc tctgttcgat
      181 atttatagcg ggcatcgaag tttccgtaat ttaacggaat tacctgtccg ttaacagtga
      241 acactatgct gttatccggg tttctctggc tgaaaaaatg ccagcctgaa tcatcaccaa
      301 gctctgcaac aacaggcctg gatggattac cccacaaa
//
LOCUS       DAJTYM010000432          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJTYM010000432 DAJTYM010000000
VERSION     DAJTYM010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.450"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>335
                     /locus_tag="OO575_004310"
     CDS             <1..>335
                     /locus_tag="OO575_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6667009.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLA"
BASE COUNT           92 a           79 c           78 g           86 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcgg
//
LOCUS       DAJTYM010000433          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJTYM010000433 DAJTYM010000000
VERSION     DAJTYM010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.451"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..106
                     /locus_tag="OO575_004311"
     CDS             <1..106
                     /locus_tag="OO575_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6667010.1"
                     /translation="KMAYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            96..269
                     /locus_tag="OO575_004312"
     CDS             96..269
                     /locus_tag="OO575_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6667011.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
BASE COUNT          111 a           67 c           75 g           81 t
ORIGIN      
        1 gaagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
       61 gtgttgctgt ctttcaagac cagacgaggt taacagtgaa tattgaagag ttaaaaaaac
      121 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      181 cagggaaaga agtctctgaa attcgcttca tcgcacgaga aaaaatgacc gggcttgaaa
      241 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
      301 ttccgcttgt tgctaattta gtgaccaatc attg
//
LOCUS       DAJTYM010000434          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJTYM010000434 DAJTYM010000000
VERSION     DAJTYM010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.452"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            235..>334
                     /gene="raiA"
                     /locus_tag="OO575_004313"
     CDS             235..>334
                     /gene="raiA"
                     /locus_tag="OO575_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6667012.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTH"
BASE COUNT          117 a           73 c           58 g           86 t
ORIGIN      
        1 gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat cgatctcatc
       61 aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct gcgatttctc
      121 acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca cacattttga
      181 catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa atttatgaca
      241 atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca tgtcgcagac
      301 cgtctcgcca aactggaaaa atggcaaaca catc
//
LOCUS       DAJTYM010000435          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJTYM010000435 DAJTYM010000000
VERSION     DAJTYM010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.453"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..236
                     /locus_tag="OO575_004314"
     CDS             <1..236
                     /locus_tag="OO575_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667013.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT           74 a           72 c          100 g           88 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtt ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gagactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcat
      301 agttcgagaa ctggttgaaa aaccatcatc aaca
//
LOCUS       DAJTYM010000436          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJTYM010000436 DAJTYM010000000
VERSION     DAJTYM010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.454"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            65..>330
                     /locus_tag="OO575_004315"
     CDS             65..>330
                     /locus_tag="OO575_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6667014.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGVDSFTMAGWIERYKTILI"
BASE COUNT          126 a           64 c           68 g           72 t
ORIGIN      
        1 ataacacacc atcagcctaa tgggcgacaa gggagagaca gtccgttaat ggagaagtta
       61 aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat atcgaaggag
      121 agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac taggtaaaat
      181 ttcaagaaga gatgcagtag cgcaggcaat tgaagcaaat cattatatat acaaaaacta
      241 ctctcctgct gccctaattg aaaagcttaa aggggtcgac tcatttacta tggcaggctg
      301 gattgaacgt tacaaaacga ttcttataag
//
LOCUS       DAJTYM010000437          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJTYM010000437 DAJTYM010000000
VERSION     DAJTYM010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.455"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            144..330
                     /locus_tag="OO575_004316"
                     /pseudo
     CDS             144..330
                     /locus_tag="OO575_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           90 a           92 c           66 g           82 t
ORIGIN      
        1 catcccgtat tgggcagtat gatgccggaa agctgcgagg ctccacacct gccagtttca
       61 cccttttaac catcatacta ctccgtgttt aattgtgtgg gatgcgttca ttacatttta
      121 acagtgtcac cccgtcggcc acgaaagata aacaggtgac cagaaaaggg atcttcatcc
      181 agcacatgct atatcttgaa cgtaacaacc tgaccctccg cacccgcatc aagcggctgg
      241 cacgtaaaac aatctgcttc tcgcgttccg tggaaatcca cgaaaagtca tcggctcctt
      301 cattgaaaaa cacatattct actgattgga
//
LOCUS       DAJTYM010000438          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJTYM010000438 DAJTYM010000000
VERSION     DAJTYM010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.456"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>325
                     /locus_tag="OO575_004317"
     CDS             <1..>325
                     /locus_tag="OO575_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="catalyzes the transfer of
                     2-keto-3-deoxy-D-manno-octulosonic acid to lipid A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HCR6667015.1"
                     /translation="AAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQHVYL
                     PYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSAAGYA
                     KLGKFV"
BASE COUNT           70 a           92 c           83 g           80 t
ORIGIN      
        1 tggcggcgat cccgttggtg cgcgcgctgc gtcatcgtta tcctgattta ccgattaccg
       61 ttacaaccat gacgccaacc ggttcggagc gcgtacaatc ggctttcggg aaggatgttc
      121 agcacgttta tctgccgtat gacctgcccg atgcgctcaa ccgtttcctg aataaagtcg
      181 accccaaact ggtgttgatt atggaaaccg aactatggcc taacctgatt gcggcgctac
      241 ataaacgtaa aattccgctg gtgatcgcta acgcgcgact ctctgcccgc tcggccgcag
      301 gttatgccaa actgggtaaa ttcgt
//
LOCUS       DAJTYM010000439          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJTYM010000439 DAJTYM010000000
VERSION     DAJTYM010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.457"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT          108 a           85 c           44 g           84 t
ORIGIN      
        1 aagcatatcg atacggcaca ttcccctgcc cctgctggca caataacatc taccagctac
       61 tcttcaaccc aacaccccaa atgcatttct ttataaattt aatctgtaac aaaaccactt
      121 cccatataga aatatttgca aaaatagtgc atgaaaaaag cttttcccta ccaatccggg
      181 atataaataa tcatcagtac cacaattatt ccacagcaat aaatctcttc ccgccgctcc
      241 ggacctctga taatggtcta aaatcattga ggccacttgc gacgacattg ctcaatgagg
      301 aaagtttggc gtgaataata t
//
LOCUS       DAJTYM010000440          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJTYM010000440 DAJTYM010000000
VERSION     DAJTYM010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.458"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            76..>321
                     /locus_tag="OO575_004318"
     CDS             76..>321
                     /locus_tag="OO575_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR6667016.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYS"
BASE COUNT           82 a           81 c           74 g           84 t
ORIGIN      
        1 ttgtctctcc gacagactga ccgaattgtt atcaatgtta acaaaaaaaa gaacaattgg
       61 ttataaggag agagtatgcg tgtcatcacc ctggcgggta gtcctcgctt tccttctcgc
      121 tccagctcct tgctggaata tgcgcgggaa aaactaaatg gcctagatgt agaggtttat
      181 cactggaatc tgcaaaactt cgccccggaa gatctgcttt atgctcgttt cgatagtccg
      241 gcactcaaga ccttcaccga acagctgcaa caggctgatg ggctgattgt cgccacgcct
      301 gtgtataaag ccgcctattc c
//
LOCUS       DAJTYM010000441          318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJTYM010000441 DAJTYM010000000
VERSION     DAJTYM010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.459"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..312
                     /locus_tag="OO575_004319"
     CDS             <1..312
                     /locus_tag="OO575_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCR6667017.1"
                     /translation="LGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVA
                     TPVLIAEMVPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTV
                     K"
BASE COUNT           69 a           74 c           93 g           82 t
ORIGIN      
        1 ctggggatcc tgttgggtgt ggcagtaatt atcgtgaccg gtattccgtt gattatcgcc
       61 gataaattga ttggcggtgg cgatggcact gccggaattg ccgcttccag ttccgcaggg
      121 gccgcggtag cgacacctgt gctgattgca gaaatggtgc ctgcgtttaa accgatggct
      181 ccggcagcaa cttcgctggt agcgacggcg gtcattgtga cttcgattct ggtgccaatt
      241 cttacctcta tctggtcacg taaagtcaaa gccagagcag cgaaaatcga aattttaggt
      301 acggtgaaat aaacctgc
//
LOCUS       DAJTYM010000442          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJTYM010000442 DAJTYM010000000
VERSION     DAJTYM010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.460"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..60)
                     /locus_tag="OO575_004320"
     CDS             complement(<1..60)
                     /locus_tag="OO575_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6667018.1"
                     /translation="MRRWISQNNIRLPRGAFFIS"
     gene            complement(35..223)
                     /locus_tag="OO575_004321"
     CDS             complement(35..223)
                     /locus_tag="OO575_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6667019.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(234..>317)
                     /locus_tag="OO575_004322"
     CDS             complement(234..>317)
                     /locus_tag="OO575_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR6667020.1"
                     /translation="TLNENQKVEFSIEQGQRGPAAANVVTL"
BASE COUNT           91 a           71 c           78 g           77 t
ORIGIN      
        1 agatataaaa aaggctccac gagggagcct gatattattt tgtgaaatcc agcggcgcat
       61 ggtcagtgcg tatttgcagc gcgacgttat caaaggtgat catagtcgat ttacaaaaaa
      121 tacacttagc gcccaaaggg tttcgctccg tcacatcgaa tgccgatgta cggtactgtg
      181 aaccatgaca acatggacag cggaaatgga gatgttgagt aataacggca accttagagc
      241 gtaacaacgt tcgctgccgc ggggccacgt tgcccctgct caatagaaaa ttcaactttc
      301 tgattttcgt tcagcgt
//
LOCUS       DAJTYM010000443          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJTYM010000443 DAJTYM010000000
VERSION     DAJTYM010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.461"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>316
                     /locus_tag="OO575_004323"
     CDS             <1..>316
                     /locus_tag="OO575_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6667021.1"
                     /translation="GSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGV
                     ATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSFAGAYANS
                     DRT"
BASE COUNT           79 a           63 c           79 g           95 t
ORIGIN      
        1 ggttcattcg actacagtcg taatgtcggt gtcgcgtatg atgcagaagc atttactgat
       61 atgtttgttg agtggggcgg tgactcatgg gctggtactg acctgttcat gaccaaccgt
      121 accaacggtg ttgcaaccta tcgtaacaca gatttctttg gcatggttga aggtctaaat
      181 tttgctctgc aatatcaggg taaaaatgaa ggtacaggca actacaaagc aaatggcgat
      241 ggccatggtt tatctgcaac ttacactatt gacggtttct catttgctgg tgcttatgca
      301 aactctgacc gtactg
//
LOCUS       DAJTYM010000444          315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJTYM010000444 DAJTYM010000000
VERSION     DAJTYM010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.462"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..124)
                     /locus_tag="OO575_004324"
     CDS             complement(<1..124)
                     /locus_tag="OO575_004324"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6667022.1"
                     /translation="MTTVTVHCPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLT"
BASE COUNT          104 a           63 c           75 g           73 t
ORIGIN      
        1 aagtgagctg gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac
       61 tacggccatg tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag
      121 tcatggaaaa cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac
      181 cttttgccgc aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg
      241 aaaaaaatta tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taatctaaat
      301 agctgcgctt aatac
//
LOCUS       DAJTYM010000445          315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJTYM010000445 DAJTYM010000000
VERSION     DAJTYM010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.463"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..303
                     /locus_tag="OO575_004325"
     CDS             <1..303
                     /locus_tag="OO575_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="starvation-sensing protein; maybe involved in
                     homoserine lactone degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional D-altronate/D-mannonate
                     dehydratase"
                     /protein_id="HCR6667023.1"
                     /translation="SLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGVQEYMGYSEQ
                     MLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGTLWNR"
BASE COUNT           76 a           78 c           82 g           79 t
ORIGIN      
        1 tcgctgtatc aggtacgtac tggctcacac ggtccttccg atttgtcacc agtctgcatg
       61 gctgcggcgc tgcactttga tctgtgggtc cccaatttcg gtgtccagga atacatgggt
      121 tattccgaac aaatgctcga agtcttcccg cacaactgga ctttcgataa cggctatatg
      181 catccgggag acaaaccggg tcttggtatc gaattcgatg aaaagctggc ggcgaaatat
      241 ccctatgaac ctgcttatct accagtcgca cgtctggaag atggcacgct gtggaacagg
      301 taaggagtaa gataa
//
LOCUS       DAJTYM010000446          314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJTYM010000446 DAJTYM010000000
VERSION     DAJTYM010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.464"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..142
                     /gene="kil"
                     /locus_tag="OO575_004326"
     CDS             <1..142
                     /gene="kil"
                     /locus_tag="OO575_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6667024.1"
                     /translation="TYIASANNIKKRKLYIRSLTTKTCISDCMIKVFLGRDGLPVKAE
                     SW"
     gene            136..312
                     /locus_tag="OO575_004327"
     CDS             136..312
                     /locus_tag="OO575_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667025.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
BASE COUNT          118 a           56 c           58 g           82 t
ORIGIN      
        1 gacatatatc gcctcggcaa acaatattaa aaagcgaaaa ctttatattc gtagcctgac
       61 tacaaaaaca tgcatttctg actgcatgat taaagtcttc ctcggtcgtg atggtttacc
      121 tgtaaaggcg gagtcatggt aatgactaag aaaataaaat gtgcttatca cctttgcaat
      181 aaagaaattg aagaaagcaa aatcattaca agaccacttc atttcatgcg tggagttata
      241 ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg ccgaaaaaga ccagatggca
      301 cacgaacttt aatt
//
LOCUS       DAJTYM010000447          314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJTYM010000447 DAJTYM010000000
VERSION     DAJTYM010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.465"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>314)
                     /locus_tag="OO575_004328"
     CDS             complement(<1..>314)
                     /locus_tag="OO575_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HCR6667026.1"
                     /translation="SRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLLLAFCVWML
                     FSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWPVFSTAIL
                     IIP"
BASE COUNT          108 a           59 c           73 g           74 t
ORIGIN      
        1 ggaataatca ggattgcagt actaaaaacc ggccatcggc gtccaccgaa tataggcacc
       61 ataaaggagt agggaacacg caataatgcg ccagaaacgg agggtaatgc ggttaataaa
      121 aagagttgat cggtagtaaa attaaaaccg attttattga gattaacggt aactgcgcta
      181 aatagcatcc agacacagaa ggcaagaagt agacaactga ctgatatcca gaggtttctt
      241 cgagcaatat gttttccttt attttcccag aaggccggat tttctggttt ccagtcgcgt
      301 aaaagataac gact
//
LOCUS       DAJTYM010000448          313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJTYM010000448 DAJTYM010000000
VERSION     DAJTYM010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.466"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT           83 a           69 c           89 g           72 t
ORIGIN      
        1 tcacccttgt gcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac
       61 acatatccct ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca
      121 aatgctcagg acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg
      181 gaaagcgttg cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa
      241 cgggaaagcc cctcccgagg aaggggcctt atagaaggaa agggttatga tgaagttcgt
      301 catcatactg gtt
//
LOCUS       DAJTYM010000449          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.467, whole genome shotgun sequence.
ACCESSION   DAJTYM010000449 DAJTYM010000000
VERSION     DAJTYM010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.467"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>312)
                     /locus_tag="OO575_004329"
     CDS             complement(<1..>312)
                     /locus_tag="OO575_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6667027.1"
                     /translation="LVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFF
                     QLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGS
                     V"
BASE COUNT           75 a           84 c           61 g           92 t
ORIGIN      
        1 agacacttcc gagccgttga taatactggt tttcatattc tgtcggtgac atctgttcgc
       61 tagaaccatg ccgacgctta ctgttataaa acatttcgat gtaatcaaaa atatcactgc
      121 gggcttcttc ccgcgttccg tagatctttt tctttatccg ttcacgtttc aacaactgga
      181 aaaaactttc tgcaaccgca ttatcatggc agttaccgcg acggctcatg ctaccctcca
      241 ggccgtgtga tttcaggaac gactgccact catggcttgt gtactgactg ccctgatccg
      301 aatgaaccag ca
//
LOCUS       DAJTYM010000450          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJTYM010000450 DAJTYM010000000
VERSION     DAJTYM010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.468"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT           79 a           73 c           83 g           77 t
ORIGIN      
        1 gcgcatgaag acggaactag tccagatgtg atatttgtgg tgatcacata catcatcaac
       61 gatgctcgtt atggtgagtt tgatgactac ccgctgaagt gaaaatagtg ttgtgtacca
      121 aattgcgtac caaactaaaa tcacaaatca tgaaaccctt gttcatggcg gttctcaggg
      181 ggattgcgcg taatcgcgaa ttcagggtat ggacccggtg tgggtcactg ctgcgatccc
      241 ggagtccatc gcctggctgg tgaaagatgc ctcgcagttt accctcaccg ttccggcgcg
      301 accggataaa ac
//
LOCUS       DAJTYM010000451          306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJTYM010000451 DAJTYM010000000
VERSION     DAJTYM010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.470"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>306)
                     /locus_tag="OO575_004330"
     CDS             complement(<1..>306)
                     /locus_tag="OO575_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6667028.1"
                     /translation="VYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYI
                     RGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGIL"
BASE COUNT           92 a           63 c           48 g          103 t
ORIGIN      
        1 tcagtatccc caggttttca cactcaatag tgactgtttt agtaatttta tgtgttgtta
       61 ccgcacttcc aacaacagat gaaaaatctg cagctggtat ttctttaaaa tcaaaacaaa
      121 ctggctttaa attatttatt tcgcaacttt gcggagctgt aatatcgccc cgaatataaa
      181 catcagccat cggctgtgag taatccaccg gggtgtcacg gctaattgtt ccatacaatg
      241 tcactatctt tgtttcagga ataatcaatt ttcctgcaaa tgtcttcttg atataaaaat
      301 atacta
//
LOCUS       DAJTYM010000452          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.471, whole genome shotgun sequence.
ACCESSION   DAJTYM010000452 DAJTYM010000000
VERSION     DAJTYM010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.471"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            44..305
                     /locus_tag="OO575_004331"
                     /pseudo
     CDS             44..305
                     /locus_tag="OO575_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          116 a           46 c           62 g           81 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tactcatacg gcatgatgat
       61 ttataaaaat gataaaacat ttcgtaatct tgaaatattc ggagacagcg gctctggagc
      121 atacttatat gataacaaac tagaaaaatg ggtattagtc ggaacaaccc atggtattgc
      181 cagcgttaat ggtgaccaac tgacatggat aacaaaatac aatgataaac tggttagtga
      241 gttaaaagat acctatagtc ataaaataaa tctgaatggc aataatgtaa ccattaaaaa
      301 cacag
//
LOCUS       DAJTYM010000453          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.472, whole genome shotgun sequence.
ACCESSION   DAJTYM010000453 DAJTYM010000000
VERSION     DAJTYM010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.472"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>303)
                     /locus_tag="OO575_004332"
     CDS             complement(<1..>303)
                     /locus_tag="OO575_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667029.1"
                     /translation="KQPQAEVLAHYLKTGDLQRVLASNSPANRILLIMGEWLAVQRRN
                     GQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTVYLFCIMLPFALV"
BASE COUNT           91 a           72 c           77 g           63 t
ORIGIN      
        1 accagcgcga acggcagcat gatacaaaac agataaacgg tacgatgcaa aatcagggtg
       61 taggcaaagg gaattggcgt gtaggcaatg cgctcacagc ctgccagaac cgctgaaata
      121 tcattaagcc gatcgttgag gctaataaac aggatatctg aaagctgtcc attgcggcgc
      181 tgaaccgcca accattctcc cattattaac aagatacggt tagctggaga gttcgaagcc
      241 agtacacgct gaagatctcc agtcttgaga taatgagcca gcacttccgc ctgtggctgt
      301 ttg
//
LOCUS       DAJTYM010000454          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.473, whole genome shotgun sequence.
ACCESSION   DAJTYM010000454 DAJTYM010000000
VERSION     DAJTYM010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.473"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(<1..>303)
                     /locus_tag="OO575_004333"
     CDS             complement(<1..>303)
                     /locus_tag="OO575_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667030.1"
                     /translation="IIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYR
                     HGSVVFWADEWKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINA"
BASE COUNT           76 a           85 c           61 g           81 t
ORIGIN      
        1 tcagcgttaa tttcagagaa tttagcaccg gcagacgcac caacaccgat agagtcgtaa
       61 acaatatctg cttcacgctc cagcgccgcc tgataagtac gctggcagct cttcagtaat
      121 tcatcttctt tggccttcca ttcgtcggcc cagaaaacaa cggatccgtg acggtaaacg
      181 ttagcgcact tatctgtacc actgtcagcc acgtcaaagc caatacgctt tcttccactg
      241 ggttcgaaat ttaacgtttt gtgcgcatcc actgcggctt ctatccaaga cagtttgatg
      301 att
//
LOCUS       DAJTYM010000455          302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.474, whole genome shotgun sequence.
ACCESSION   DAJTYM010000455 DAJTYM010000000
VERSION     DAJTYM010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.474"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>302
                     /gene="curA"
                     /locus_tag="OO575_004334"
     CDS             <1..>302
                     /gene="curA"
                     /locus_tag="OO575_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6667031.1"
                     /translation="GRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGY
                     SGWQDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVV"
BASE COUNT           59 a           63 c          105 g           75 t
ORIGIN      
        1 gtggacgtat gagcgatgag ccatcttatt caccgccggt tgatattggc ggcgtgatgg
       61 tcggcggtac ggtgagccgt gtcgtggagt cgaatcatcc tgattatcag cctggcgact
      121 gggtgctggg ctacagtgga tggcaggact atgacatatc cagtggtgat gacctggtga
      181 aacttggcga tcatccgcaa aatccatcgt ggtcgctggg tgtgctgggg atgccaggct
      241 ttaccgctta tatggggctg ctggatatcg gtcagcctaa agagggcgaa acgttggtgg
      301 ta
//
LOCUS       DAJTYM010000456          300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.475, whole genome shotgun sequence.
ACCESSION   DAJTYM010000456 DAJTYM010000000
VERSION     DAJTYM010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.475"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
BASE COUNT           59 a           82 c           84 g           75 t
ORIGIN      
        1 tgattattgg ctctggcgac ggctttcaga acctctttca ccattctggt gtggactttc
       61 tggtgctcca ggttgtgtga catgggaact cattctggat ggttactctg aaagctcata
      121 ttctgccaca ccccgatttg cagccgccag gctgccgtgg ttcacagata aaacactctc
      181 caggaaaccc ggggcggttc actgcgtgtc tgggttcgcc agcatgagcg ggataccggg
      241 ggcggttcac acgaacatac cacagacagt tgattttggt acgcccaggc gacgtccggc
//
LOCUS       DAJTYM010000457          295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.denovo.476, whole genome shotgun sequence.
ACCESSION   DAJTYM010000457 DAJTYM010000000
VERSION     DAJTYM010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.denovo.476"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..>295
                     /locus_tag="OO575_004335"
     CDS             <1..>295
                     /locus_tag="OO575_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6667032.1"
                     /translation="PMVERPVRRCEIQWLNNIYYAPELRDEHGRKVLISYDIHDAERI
                     TVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRKRAEEKIRDAEDEG"
BASE COUNT           90 a           62 c           82 g           61 t
ORIGIN      
        1 ccaatggtgg aacgaccggt aagacgctgc gaaattcagt ggctgaacaa tatttactac
       61 gcgccggagt tgcgtgacga acacggtcgc aaagtgctta tcagttatga cattcatgac
      121 gccgagcgaa ttacggtacg tcgcaaggac ggcagcttta tctgtgaggc gatatggaac
      181 ggcaataaac gcgcagcctt tgctgtcagt gctgaatatc acaaacaaca gcagcgtatt
      241 aaaggaatgc gcaaacgcgc tgaggaaaaa attcgtgatg ctgaagatga aggta
//
LOCUS       DAJTYM010000458         6007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.164, whole genome shotgun sequence.
ACCESSION   DAJTYM010000458 DAJTYM010000000
VERSION     DAJTYM010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.164"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            20..1828
                     /gene="frdA"
                     /locus_tag="OO575_004336"
     CDS             20..1828
                     /gene="frdA"
                     /locus_tag="OO575_004336"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6667033.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            1821..2555
                     /gene="frdB"
                     /locus_tag="OO575_004337"
     CDS             1821..2555
                     /gene="frdB"
                     /locus_tag="OO575_004337"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6667034.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2566..2961
                     /gene="frdC"
                     /locus_tag="OO575_004338"
     CDS             2566..2961
                     /gene="frdC"
                     /locus_tag="OO575_004338"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6667035.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2972..3331
                     /gene="frdD"
                     /locus_tag="OO575_004339"
     CDS             2972..3331
                     /gene="frdD"
                     /locus_tag="OO575_004339"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6667036.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            3394..4527
                     /locus_tag="OO575_004340"
     CDS             3394..4527
                     /locus_tag="OO575_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6667037.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4616..5149
                     /gene="blc"
                     /locus_tag="OO575_004341"
     CDS             4616..5149
                     /gene="blc"
                     /locus_tag="OO575_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6667038.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5146..5463)
                     /gene="sugE"
                     /locus_tag="OO575_004342"
     CDS             complement(5146..5463)
                     /gene="sugE"
                     /locus_tag="OO575_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6667039.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5639..5785)
                     /gene="ecnB"
                     /locus_tag="OO575_004343"
     CDS             complement(5639..5785)
                     /gene="ecnB"
                     /locus_tag="OO575_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6667040.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(5896..>6007)
                     /gene="ecnA"
                     /locus_tag="OO575_004344"
     CDS             complement(5896..>6007)
                     /gene="ecnA"
                     /locus_tag="OO575_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6667041.1"
                     /translation="IVLVLLASTLLTGRNTACGFGEDIKHLGNSISRAAS"
BASE COUNT         1480 a         1563 c         1600 g         1364 t
ORIGIN      
        1 aacaatctgg aggaatgtcg tgcaaacctt tcaagccgat cttgccattg taggcgccgg
       61 tggcgcggga ttacgtgctg caattgctgc cgcgcaggca aatccaaatg caaaaatcgc
      121 actaatctca aaagtatacc cgatgcgtag ccataccgtt gctgcagaag ggggctccgc
      181 cgctgtcgcg caggatcatg acagcttcga atatcacttt cacgatacag tagcgggtgg
      241 cgactggttg tgtgagcagg atgtcgtgga ttatttcgtc caccactgcc caaccgaaat
      301 gacccaactg gaactgtggg gatgcccatg gagccgtcgc ccggatggta gcgtcaacgt
      361 acgtcgcttc ggcggcatga aaatcgagcg cacctggttc gccgccgata agaccggctt
      421 ccatatgctg cacacgctgt tccagacctc tctgcaattc ccgcagatcc agcgttttga
      481 cgaacatttc gtgctggata ttctggttga tgatggtcat gttcgcggcc tggtagcaat
      541 aaacatgatg gaaggcacgt tggtgcagat ccgtgctaac gcggtcgtta tggctaccgg
      601 cggtgcgggt cgcgtttatc gttacaacac caacggcggc atcgttaccg gtgacggtat
      661 gggtatggcg ctaagccacg gcgttccgct gcgtgacatg gaattcgttc agtatcaccc
      721 aaccggtctg ccaggttccg gtatcctgat gaccgaaggc tgccgtggtg aaggcggtat
      781 tctggtcaac aaaaatggct accgttatct gcaagattac ggcatgggcc cggaaactcc
      841 gctgggcgag ccgaaaaaca aatatatgga actgggtcca cgcgacaaag tttctcaggc
      901 cttctggcac gaatggcgta aaggcaacac catctccacg ccgcgtggtg atgtggttta
      961 tctcgacctg cgtcacctcg gcgagaaaaa actgcatgaa cgtctgccgt tcatctgcga
     1021 actggcgaaa gcgtacgttg gcgtcgatcc ggttaaagaa ccgattccgg tacgtccgac
     1081 tgcacactac accatgggcg gtatcgaaac cgatcagaac tgtgaaaccc gcattaaagg
     1141 tctgttcgcc gtgggtgaat gttcctctgt tggtctgcac ggtgcaaacc gtctgggctc
     1201 caactccctg gcggaactgg tggtcttcgg ccgtctggcc ggtgaacaag cgacagagcg
     1261 tgcagcaact gccggtaatg gcaacgaagc ggcaattgaa gcgcaggcag ctggcgttga
     1321 acaacgtctg aaagatctag ttaaccagga tggcggcgaa aactgggcga agatccgcga
     1381 cgaaatgggc ctggcaatgg aagaaggttg cggtatctac cgtacgccgg aactgatgca
     1441 gaaaaccatc gacaagctgg cagagctgca ggaacgcttc aagcgcgtgc gcatcaccga
     1501 cacctccagc gtgttcaaca ccgacctgct ctacaccatt gaactgagcc acggtctgaa
     1561 cgttgctgaa tgtatggcgc actccgcaat ggcacgtaaa gagtcccgcg gcgcacacca
     1621 gcgtctggac gaaggttgca ccgagcgtga cgacgtcaac tttctcaaac acaccctcgc
     1681 cttccgcgat gctgatggca cgactcgcct ggagtacagc gacgtgaaga ttactacgct
     1741 gccgccagct aaacgcgttt acggtggcga agcggatgca gccgataagg cggaagcagc
     1801 caataataag gagaaggcga atggctgaga tgaaaaacct gaaaattgag gtggtgcgct
     1861 ataacccgga agtcgatacc gcaccgcata gcgcattcta tgaagtgcct tatgacgcaa
     1921 ctacctcatt actggatgcg ctgggctaca tcaaagacaa cctggcaccg gacctgagtt
     1981 accgctggtc ctgccgtatg gcgatttgtg gttcctgcgg catgatggtt aacaacgtgc
     2041 caaaactggc atgtaaaacc ttcctgcgtg attacaccga cggtatgaag gttgaagcgt
     2101 tagctaactt cccgattgaa cgcgatctgg tggtcgatat gacccacttc atcgaaagtc
     2161 tggaagcgat caaaccgtac atcatcggca actcccgcac cgcggatcag ggtactaaca
     2221 tccagacccc ggcgcagatg gcaaagtatc accagttctc cggttgcatc aactgtggtc
     2281 tgtgctacgc cgcgtgcccg cagtttggcc tgaacccaga gttcatcggt ccggctgcca
     2341 ttacgctggc gcatcgttat aacgaagata gccgcgacca cggtaagaag gagcgtatgg
     2401 cgcagttgaa cagccagaac ggtgtatgga gctgtacttt cgtgggctat tgctccgaag
     2461 tctgcccgaa acacgtcgat ccggctgcgg ccattcagca gggcaaagta gaaagttcga
     2521 aagactttct tatcgcgacc ctgaaaccac gctaaggagt gcaacatgac gactaaacgt
     2581 aaaccgtatg tacggccaat gacgtccacc tggtggaaaa aattgccgtt ttatcgcttt
     2641 tacatgctgc gcgaaggcac ggcggttccg gctgtgtggt tcagcattga actgattttc
     2701 gcgctgtttg ccctgaaaaa tggcccggaa gcctgggcgg gattcgtcga ctttttacaa
     2761 aacccggtta tcgtgatcat taacctgatc actctgacgg cagctctgct gcacaccaaa
     2821 acctggtttg aactggcacc gaaagcggcc aatatcattg taaaagacga aaaaatggga
     2881 ccagagccaa ttatcaaaag tctctgggcg gtaactgtgg ttgccaccat cgtaatcctg
     2941 tttgttgccc tgtactggta aggagcctga gatgattaat ccaaatccaa agcgttctga
     3001 cgaaccggta ttctggggcc tcttcggggc cggtggtatg tggagcgcaa ttattgcgcc
     3061 ggtgatgatc ctgctggtgg gtattctgct gccactgggg ctgtttccgg gtgatgcgct
     3121 gagctacgag cgcgttctgg cgttcgcgca gagcttcatt ggtcgcgtat tcctgttcct
     3181 gatgatcgtt ctgccgctgt ggtgtggttt acaccgtatg caccacgcga tgcacgatct
     3241 gaaaatccac gtgcctgcgg gcaaatgggt tttctacggt ctggctgcta tcctgacagt
     3301 tgtcacgctg attggtgtcg ttacaatcta acgcatcgcc aatgtaaatc cggcccgcct
     3361 atggcgggcc gttttgtatg gaaaccagac cttatgttca aaacgacgct ctgcgcctta
     3421 ttaattaccg cctcttactc cacatttgct gcccctcaac aaatcaacga tattgtgcat
     3481 cgcacaatta ccccgcttat agagcaacaa aagatccccg gtatggcggt ggcggtaatt
     3541 tatcagggta aaccttatta ctttacctgg ggctatgcgg acatcgccaa aaagcagccc
     3601 gtcacacagc aaacgttgtt tgagttaggt tcggtcagca aaacatttac gggcgtgctt
     3661 ggtggcgacg ctattgctcg aggggaaatc aagttaagcg atcccgcaac aaaatactgg
     3721 cctgaactta ccgctaaaca gtggaatggg atcacactat tacatcttgc gacctacacc
     3781 gctggcggcc tgccattgca ggtgccggat gaagtgaaat cctcaagcga cttgctgcgc
     3841 ttctatcaaa actggcagcc tgcatgggct ccaggaacac aacgtctgta tgccaactcc
     3901 agtatcggtt tgttcggcgc actggctgtg aagccgtctg gtttgagttt tgagcaggcg
     3961 atgcaaactc gtgtcttcca gccactcaaa ctcaaccata cgtggattaa tgtacctccc
     4021 ccagaagaaa agaattacgc ctggggatat cgcgaaggta aggcagtgca tgtttcgcca
     4081 ggggcgttag atgctgaagc ttatggtgtg aagtcgacca ttgaagatat ggcccgctgg
     4141 gtgcaaagca atttaccccc ccttgatatc aatgagaaaa cgcttcaaca agggatacaa
     4201 ctggcacaat ctcgctactg gcaaaccggc gatatgtatc agggcctggg ctgggaaatg
     4261 ctggactggc cggtcaatcc tgacagcatc attaacggca gtgacaataa aattgcactg
     4321 gcagcacgcc cggtaaaagc gattaagccc ccaactcctg cagtacgcgc atcatgggta
     4381 cataaaacgg gggcgaccgg cggatttggt agctatgtcg cgtttattcc agaaaaagag
     4441 ctgggtatcg tgatgctggc taacaaaaac tatcccaatc cagcgagagt cgccgccgcc
     4501 tgtcagattc tcaacgctct acagtaaaat ttcatcgggt ccgaattttc ggaccttttc
     4561 tgcgcttttc cttgctgtca tctacactta gaaaaaaacc agtaaggaaa catttatgcg
     4621 cctgctccct ctcgttgccg cagcgacagc tgcatttctg gtcgttgcct gtagttttcc
     4681 tacgccgccg cgtggcgtga ccgtagtgaa taatttcgac gccaaacgtt atctcggtac
     4741 ctggtatgag attgcccgtt ttgatcaccg ttttgaacgt ggactggaaa aagtcaccgc
     4801 aacatacagc ctgcgtgatg acggcggcct gaatgtcatt aataaaggct ataaccctga
     4861 cagaggaatg tggcagcaga gtgaagggaa agcgtacttt accggcgcac caactcgcgc
     4921 tgcgctgaaa gtgtcattct ttggtccttt ctatggcggt tataacgtta ttgcactcga
     4981 tcgggaatac cgccatgcgc tggtttgcgg cccggaccgc gactacctgt ggatactctc
     5041 ccgcacgcca accatttctg acgaagtgaa acaggagatg ctggcagtcg cgacccggga
     5101 agggtttgat gtcagtaagt ttatttgggt acagcagccc ggtagttagt gagtgctgag
     5161 tttcagacca ataatcccca atacgattaa cgccagactc gccagacgca tcgggttagc
     5221 ggactcaccg agcagcacaa tgccggtgat ggccgcgccg actgcgccaa tacccgtcca
     5281 cacggcataa gccgtcccta ccggtaacga tttcatcgcc caggcaagta gcgccatact
     5341 gactatcatc gccgtcacgg taataacact cggcgtcaaa cggctaaagc cgtgggtata
     5401 tttcaggcca acggcccata ccacttccag cagaccagca ataactaaga taatccagga
     5461 catatcaggc tccggaaatt tggggccgtc cccggtgaaa agaggcgttt gcaggtcgtc
     5521 ctgcaaagct gatggttgaa cggatatttt gccaggtggc aggggaattt caactattac
     5581 ctttatgacg atcgcagaaa aaacaaacgg cacgacactg ctgtcgtacc gttattgctt
     5641 attgctgcgc tttcgttgct gcgccagaaa tcgcgttacc gccatcagaa atgtcttcac
     5701 caacgccacg cgtggtgttg caggcagtta atactgttga aagcaccaga acagaaaaga
     5761 tcgctgcaat tgtcttcttc accataacgt cttcctttta ggtaatgtta tttatgtttg
     5821 cctatagcaa attaataata gacaacatcg ccaaaaatga tggaaatctg atgatttttc
     5881 ggaagagaag aaaaattagc tggcagcgcg agagatggag ttaccgagat gtttgatgtc
     5941 ttcgccgaaa ccgcaagcgg tgttacggcc cgtgagcagc gtgctggcaa gcaaaacaag
     6001 aacgata
//
LOCUS       DAJTYM010000459         4862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.180, whole genome shotgun sequence.
ACCESSION   DAJTYM010000459 DAJTYM010000000
VERSION     DAJTYM010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.180"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(2..1459)
                     /gene="pepD"
                     /locus_tag="OO575_004345"
     CDS             complement(2..1459)
                     /gene="pepD"
                     /locus_tag="OO575_004345"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6667042.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            1721..2179
                     /gene="gpt"
                     /locus_tag="OO575_004346"
     CDS             1721..2179
                     /gene="gpt"
                     /locus_tag="OO575_004346"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6667043.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2271..3515
                     /gene="frsA"
                     /locus_tag="OO575_004347"
     CDS             2271..3515
                     /gene="frsA"
                     /locus_tag="OO575_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6667044.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3573..3974
                     /gene="crl"
                     /locus_tag="OO575_004348"
     CDS             3573..3974
                     /gene="crl"
                     /locus_tag="OO575_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6667045.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(4013..>4862)
                     /gene="phoE"
                     /locus_tag="OO575_004349"
     CDS             complement(4013..>4862)
                     /gene="phoE"
                     /locus_tag="OO575_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6667046.1"
                     /translation="QLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKYKDLGSFDYG
                     RNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQ
                     YQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAE
                     AWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYV
                     LSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDTVAV
                     GMTYQF"
BASE COUNT         1165 a         1220 c         1229 g         1248 t
ORIGIN      
        1 attacttcgc cggaatttct ttcagcagtt cagtcagcag tgtccagtaa tgacctacgc
       61 tttcgatgtg aacttgctca tccggagagt gtggaccggt gatagttggc ccgatagaaa
      121 ccatgtccat ttccggatac ggttttttga acagaccaca ttccaggccc gcgtggataa
      181 tctggatgtt cggcgtcttg ttgaacaggc gctgataggt ttcacgtacc agatgcatca
      241 ccggagaatt agcgtccggc tgccagccag gatatgcgcc tttcgcttcg gttttcgcgc
      301 cagccagttt acccagcgaa tccagcatgc tcaccacgta gtctttaccg ctgtcgatta
      361 gtgaacggat caggcagtga atttctacgt tattgtcagt catggtcacc acaccgacgt
      421 tcagggaggt ttcgaccaca ccttttgcca cgtcggaatt gcggatcaca ccgttcgggg
      481 tggcgttcag cagacgaata aaggtatcgc gagatttcgc aatcagggca gctttatcgt
      541 tcgctacaga gtccagcaac aaggccagat ttttctcttt ctctgccagc tcgtttttca
      601 ggatctcctg ataggtattc accagagatt tcagtgcgtc gactttatca gctgcgacag
      661 caatggtcgc aaaggcttca cgcgggatgg cgttacgcag tgtgccgccg ttgaaatcga
      721 taaggcgcag gtccagttct tctgcatgac ccgccaggaa gcgcaccagc agtttgttgg
      781 cattacccag gccaacgtgg atttcaccgc cggagtgacc gcctttcaga ccttttaagg
      841 ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg atctaaatgc aggttggagg
      901 tgaagtcgat accccccgca caacccatgt agatttcacc ttcttcttcg gagtcggtgt
      961 taatcagaat atcagcctgc aaccagttgc tctgtaagcc gaacgcaccg tccataccgg
     1021 cttcttcggt catggtcagc agcacttcca gcgggccgtg aaccacgttt tcgtcagcca
     1081 gaaccgccag tgcagaggcc ataccaatgc cgttatccgc acccagcgtg gtgccgcgcg
     1141 ctttaaccca ttcgccatca atataaggct ggataggatc tttcgtgaag tcatgcacgg
     1201 tgtcgttatt tttctgcggc accatatcga ggtgggcctg taagacgacc ggtttacgat
     1261 tttccatacc tgcggtagca ggttgacgaa tcaggatatt acctacctga tcgcgttcga
     1321 catggaaacc tttctctttt gcccaaccaa caatgtattc agcgagttgc tcttcatgat
     1381 aggacgggtg aggaatagaa cagattttgg caaaaatatc ccacagcggc tgtggagata
     1441 attgagacag ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac agtattgcag
     1501 gtcacagggt tagcagaaaa tgttgtcaac acaagacagg cttgcgagat atgtattgag
     1561 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     1621 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     1681 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     1741 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     1801 acaatggaaa ggcattattg ccgtaagccg tggcggtctg gtaccggggg cgttactggc
     1861 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     1921 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     1981 tgatgacctg gtggataccg gtggtactgc tgttgcgatt cgtgaaatgt atccaaaagc
     2041 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
     2101 tgatatcccg caagatacct ggatcgaaca gccgtgggat atgggcgtcg tattcgtccc
     2161 gccaatctcc ggtcgctaat cttttcaacg cctggcattg ccgggcgttg ttctttttaa
     2221 cttcaggcag gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     2281 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
     2341 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggctcttgat ggtaaaacca
     2401 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggt attgacccac
     2461 gtgaaatcct cgacgtccag gcacgtattg tgatgagcga tgctgaacgt accgacgatg
     2521 atttatacga taccgtgatt ggctaccgtg gtggcaactg gatttatgag tgggccaccc
     2581 aggcgatggt gtggcaacaa aaagcctgta cggaagaaga tccgcaactc agtggtcgac
     2641 actggctgca tgcggctacg ttgtacaaca ttgccgccta tcctcatctg aaaggagatg
     2701 acctggccga gcaagcgcag gctttatcaa accgcgccta tgaagaggcc gcccagcgtc
     2761 tgccgggcac gatgcggcag atggagttta ccgtacccgg cggtgcgccc atcaccggct
     2821 ttttgcatat gccgaaaggc gatggcccgt tcccgacagt attaatgtgt ggtggtctgg
     2881 atgcgatgca gacggactat tacagcctgt atgaacgtta ttttgcgccg cgcggcattg
     2941 cgatgctgac tattgatatg ccgtcggtgg gcttttcttc aaaatggaag ctcacccagg
     3001 actccagcct gttgcatcag cacgtcttaa aggcgctgcc taacgtaccg tgggtggatc
     3061 acactcgcgt cgcggccttt ggtttccgtt tcggcgctaa cgttgccgtg cgtctggcat
     3121 accttgaatc gccgcgtctg aaagcggttg cctgtcttgg tccggtagtt cataccctgt
     3181 tgagtgattt taagtgccag caacaggtgc cggaaatgta tcttgacgtt ctggcgagtc
     3241 gtttggggat gcatgatgct tccgacgaag cgttacgcgt ggagctgaat cgctattcat
     3301 taaaagtgca aggattgctg gggcgtcgct gcccaacgcc aatgttatca ggctactgga
     3361 agaacgatcc gttcagcccg gaagaggact cacgcttaat cacctcatca tctgctgacg
     3421 gtaaattatt agagatccca tttaacccgg tgtatcggaa ttttgacaaa ggtcttcagg
     3481 aaatcaccgg ctggatcgaa aaacgcttgt gttaaaaatt tgctaaattt tgccaatttg
     3541 gtaaaacagt tgcatcacaa caggagatag caatgacgtt accgagtgga cacccgaaga
     3601 gcagattgat caaaaaattt accgcactag gcccgtatat tcgtgaaggt aagtgcgaag
     3661 ataatcgatt ctttttcgat tgtctggctg tatgcgtcaa cgtgaaaccg gcaccggaag
     3721 tgcgtgaatt ctggggctgg tggatggagc tggaagcgca ggaatcccgt tttacctaca
     3781 gctaccagtt tggtctgttc gataaagcag gcgactggaa gagtgttccg gtaaaagata
     3841 ctgaagtggt tgaacgactg gagcacaccc tgcgtgagtt tcacgagaag ctgcgtgaac
     3901 tgctgacgac gctgaatctg aagctggaac cggcgaatga ttttcgtgat gaaccggtga
     3961 agttaacggc gtgagtgaaa tgtgccggat gcatcacatc cggcaatatt cattaaaact
     4021 gatacgtcat cccaaccgcg acagtatcat cattattaat attcagctta ttatcgctat
     4081 ccagttggtt gattttataa tcaacaaacg ctgacatatt tttgttgaaa taatacgtag
     4141 caccgacgtc gatataattg accagatctt catcaccgat gccctcaata tctttccctt
     4201 tcgataagac ataacccagc gatggacgca gaccaaagtc aaactggtat tgagcgaccg
     4261 cttcaaagtt ctgtgtctta ttggcaaagc cgccagttat tggcgtcatt ttgcgtgttt
     4321 cagaatagaa agttgccaga taaatattat tggcatcgta tttcagacct gttgcccatg
     4381 cttctgcacg cttgcctgtg ccacggcttt gcaggttctg ctcgttggtg cgatctgagt
     4441 tggtataggc cccactaatg gcgaaatcgc tgccgccaaa gtcatatgtc aatgacgtgc
     4501 cgaagccatc gccgttttgc tttttaacgt cgcggttttc gtttttccct tgatattgca
     4561 gggttaagtt caggccatcg ataacgccga agaagtcggt gttccgatac gtcgccagac
     4621 cgctggcgcg tttggtcata aagttgtcgg tctgcgccga ggaatcgcca ccaaattccg
     4681 ggaacatatc ggtccaggct tccacgtcat acaacgcgcc caggttacga ccataatcga
     4741 aagaacccaa atctttatat ttcaacccgg caaaagcgag acgcgttttt tgctgtgcag
     4801 tatcactctc cgctttattt ccggcaaact ccgcttccca ccgaccataa ccagtcagtt
     4861 ga
//
LOCUS       DAJTYM010000460         3296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.208, whole genome shotgun sequence.
ACCESSION   DAJTYM010000460 DAJTYM010000000
VERSION     DAJTYM010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.208"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..155
                     /locus_tag="OO575_004350"
     CDS             <1..155
                     /locus_tag="OO575_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6667047.1"
                     /translation="SERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAF
                     KTHFHI"
     gene            complement(133..756)
                     /gene="tetR(B)"
                     /locus_tag="OO575_004351"
     CDS             complement(133..756)
                     /gene="tetR(B)"
                     /locus_tag="OO575_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6667048.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            838..2043
                     /gene="tet(B)"
                     /locus_tag="OO575_004352"
     CDS             838..2043
                     /gene="tet(B)"
                     /locus_tag="OO575_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6667049.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2156..2749)
                     /gene="tetC"
                     /locus_tag="OO575_004353"
     CDS             complement(2156..2749)
                     /gene="tetC"
                     /locus_tag="OO575_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6667050.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2882..3253
                     /locus_tag="OO575_004354"
     CDS             2882..3253
                     /locus_tag="OO575_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6667051.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          889 a          600 c          683 g         1124 t
ORIGIN      
        1 ggagtgaacg ccgtttccat ttaggtgggt acgttggagc cgcattattt tcgctttatg
       61 aatctaaagg gtggttaact cgacatcttg gttaccgtga agttaccatc acggaaaaag
      121 gttatgctgc ttttaagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
      181 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
      241 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
      301 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
      361 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
      421 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
      481 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
      541 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
      601 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
      661 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
      721 gctgttaatc actttacttt tatctaatct agacatcatt aattcctaat ttttgttgac
      781 actctatcat tgatagagtt attttaccac tccctatcag tgatagagaa aagtgaaatg
      841 aatagttcga caaagatcgc attggtaatt acgttactcg atgccatggg gattggcctt
      901 atcatgccag tcttgccaac gttattacgt gaatttattg cttcggaaga tatcgctaac
      961 cactttggcg tattgcttgc actttatgcg ttaatgcagg ttatctttgc tccttggctt
     1021 ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt aataggcgca
     1081 tcgctggatt acttattgct ggctttttca agtgcgcttt ggatgctgta tttaggccgt
     1141 ttgctttcag ggatcacagg agctactggg gctgtcgcgg catcggtcat tgccgatacc
     1201 acctcagctt ctcaacgcgt gaagtggttc ggttggttag gggcaagttt tgggcttggt
     1261 ttaatagcgg ggcctattat tggtggtttt gcaggagaga tttcaccgca tagtcccttt
     1321 tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg ttatgttttg gttccgtgaa
     1381 accaaaaata cacgtgataa tacagatacc gaagtagggg ttgagacgca atcgaattcg
     1441 gtatacatca ctttatttaa aacgatgccc attttgttga ttatttattt ttcagcgcaa
     1501 ttgataggcc aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg ttttggatgg
     1561 aatagcatga tggttggctt ttcattagcg ggtcttggtc ttttacactc agtattccaa
     1621 gcctttgtgg caggaagaat agccactaaa tggggcgaaa aaacggcagt actgctcgga
     1681 tttattgcag atagtagtgc atttgccttt ttagcgttta tatctgaagg ttggttagtt
     1741 ttccctgttt taattttatt ggctggtggt gggatcgctt tacctgcatt acagggagtg
     1801 atgtctatcc aaacaaagag tcatcagcaa ggtgctttac agggattatt ggtgagcctt
     1861 accaatgcaa ccggtgttat tggcccatta ctgtttgctg ttatttataa tcattcacta
     1921 ccaatttggg atggctggat ttggattatt ggtttagcgt tttactgtat tattatcctg
     1981 ctatcgatga ccttcatgtt aacccctcaa gctcagggga gtaaacagga gacaagtgct
     2041 tagttatttc gtcaccaaat gatgttattc cgcgaaatat aatgaccctc ttgataaccc
     2101 aagagggcat tttttacgat aaagaagatt tagcttcaaa taaaacctat ctattttatt
     2161 tatctttcaa gctcaataaa aagccgcggt aaatagcaat aaattggcct tttttatcgg
     2221 caagctcttt taggtttttc gcatgtattg cgatatgcat aaaccagcca ttgagtaagt
     2281 ttttaagcac atcatcatca taagctttaa gttggttctc ttggatcaat ttgctgacaa
     2341 tggcgtttac cttaccagta atgtattcaa ggctaatttt ttcaagttca ttccaaccaa
     2401 tgataggcat cacttcttgg atagggataa ggtttttatt attatcaata atataatcaa
     2461 gataatgttc aaatatactt tctaaggcag accaaccatt tgttaaatca gtttttgttg
     2521 tgatgtaggc atcaatcata attaattgct gcttataaca ggcactgagt aattgttttt
     2581 tatttttaaa gtgatgataa aaggcacctt tggtcaccaa cgcttttccc gagatctcat
     2641 ctattgaaac agcttgatag cctttttcaa caaacaatat tcgtgctgag ttaaccagtg
     2701 attgataggt actcttaaaa ttttcttgtt gatgattttt attttccatg atagatttaa
     2761 aataacatac cgtcagtatg tttatggtat catgatgatg tggtcgtgac aatcttaaga
     2821 acatttaggt tattttatgt atattgaaca gcattctcgc tatcaaaata aagctaataa
     2881 catccaatta agatatgatg ataagcagtt tcatacaacg gttatcaaag atgttctatt
     2941 atggattgaa cataatttag atcagtcttt actgcttgat gatgtggcga ataaagcggg
     3001 ttataccaag tggtattttc agcggctgtt caaaaaagta acaggggtca cactggctag
     3061 ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag ttgaggttga cgaaaaaaac
     3121 tatccttgag atcgcattaa aatatcaatt tgattcccaa caatctttta cacgtcgatt
     3181 taagtacatt tttaaggtta caccaagtta ttatcggcgt aataaattat gggaattgga
     3241 ggcaatgcac tgagagggtg atgctgccaa cttactgatt tagtgtatga tggtgt
//
LOCUS       DAJTYM010000461         2817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.214, whole genome shotgun sequence.
ACCESSION   DAJTYM010000461 DAJTYM010000000
VERSION     DAJTYM010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.214"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            complement(48..398)
                     /locus_tag="OO575_004355"
     CDS             complement(48..398)
                     /locus_tag="OO575_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6667052.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(601..1614)
                     /gene="intI1"
                     /locus_tag="OO575_004356"
     CDS             complement(601..1614)
                     /gene="intI1"
                     /locus_tag="OO575_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6667053.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1824..2654
                     /locus_tag="OO575_004357"
     CDS             1824..2654
                     /locus_tag="OO575_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6667054.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          755 a          744 c          717 g          601 t
ORIGIN      
        1 cctgacgcaa tttacacatg gcatctgaca tcaggttagg gtatgcctca acctgacggc
       61 ggcgaatcac aagcgtccgg tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg
      121 aagcgctgat agcactccag cccgcagaaa tgctccacgt actcggcccc ttccggcgtg
      181 aaggcggcat cgagcgggat ttccttgcag cacacgcagc aactggtgct cggttcattg
      241 gcgttcatgg tggtgttcct ccattggttg acgaagccga cgaaggccgc cgccggcatc
      301 ggcctggcga acaggaaacc ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg
      361 cagtcgggtg tttccacacc ctcggctacc acctccattg tcgttttcag aagacggctg
      421 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac
      481 gacgggctgc tgccggccat cagcggacgc agggaggact ttccgcaacc ggccgttcga
      541 tgcggcaccg atggccttcg cgcaggggta gtgaatccgc caggattgac ttgcgctgcc
      601 ctacctctca ctagtgaggg gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc
      661 aactttcagc acatgcgtgt aaatcatcgt cgtagagacg tcggaatggc cgagcagatc
      721 ctgcacggtt cgaatgtcgt aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag
      781 ggtgtgcggt gtggcgggct tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg
      841 ctgaaaggtc tggtcataca tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga
      901 atgcgtgtgc tgcgcaaaaa cccagaacca cggccaggaa tgcccggcgc gcggatactt
      961 ccgctcaagg gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca
     1021 ccatgcccgt gcacgcgaca gctgctcgcg caggctgggt gccaagctct cgggtaacat
     1081 caaggcccga tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc
     1141 cagatccttg acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag
     1201 ctgggcgaac aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg
     1261 ggtcagcacc accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg
     1321 cagatccgtg cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg
     1381 cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct
     1441 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     1501 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     1561 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     1621 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     1681 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     1741 ccctaaaaca aagttgggcg aacccggagc ctcattaatt gttagccgtt aaaattaagc
     1801 cctttaccaa accaatactt attatgaaaa acacaataca tatcaacttc gctatttttt
     1861 taataattgc aaatattatc tacagcagcg ccagtgcatc aacagatatc tctactgttg
     1921 catctccatt atttgaagga actgaaggtt gttttttact ttacgatgca tccacaaacg
     1981 ctgaaattgc tcaattcaat aaagcaaagt gtgcaacgca aatggcacca gattcaactt
     2041 tcaagatcgc attatcactt atggcatttg atgcggaaat aatagatcag aaaaccatat
     2101 tcaaatggga taaaaccccc aaaggaatgg agatctggaa cagcaatcat acaccaaaga
     2161 cgtggatgca attttctgtt gtttgggttt cgcaagaaat aacccaaaaa attggattaa
     2221 ataaaatcaa gaattatctc aaagattttg attatggaaa tcaagacttc tctggagata
     2281 aagaaagaaa caacggatta acagaagcat ggctcgaaag tagcttaaaa atttcaccag
     2341 aagaacaaat tcaattcctg cgtaaaatta ttaatcacaa tctcccagtt aaaaactcag
     2401 ccatagaaaa caccatagag aacatgtatc tacaagatct ggataatagt acaaaactgt
     2461 atgggaaaac tggtgcagga ttcacagcaa atagaacctt acaaaacgga tggtttgaag
     2521 ggtttattat aagcaaatca ggacataaat atgtttttgt gtccgcactt acaggaaact
     2581 tggggtcgaa tttaacatca agcataaaag ccaagaaaaa tgcgatcacc attctaaaca
     2641 cactaaattt ataaaaaatc taatggcaaa atcgcccaac ccttcaatca agtcgggacg
     2701 gccaaaagca agcttttggc tcccctcgct ggcgctcggc gccccttatt tcaaacgtta
     2761 aacatcatga gggaagtggt gatcgccgaa gtatcgactc aactatcaga ggtagtt
//
LOCUS       DAJTYM010000462         1255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.265, whole genome shotgun sequence.
ACCESSION   DAJTYM010000462 DAJTYM010000000
VERSION     DAJTYM010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.265"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            55..528
                     /locus_tag="OO575_004358"
     CDS             55..528
                     /locus_tag="OO575_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6667055.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            623..1147
                     /gene="sat2"
                     /locus_tag="OO575_004359"
     CDS             623..1147
                     /gene="sat2"
                     /locus_tag="OO575_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6667056.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT          373 a          279 c          291 g          312 t
ORIGIN      
        1 gggtgacaaa acgagcatgc ttactaataa aatgttaacc tctgaggaag aattgtgaaa
       61 ctatcactaa tggtagctat atcgaagaat ggagttatcg ggaatggccc tgatattcca
      121 tggagtgcca aaggtgaaca gctcctgttt aaagctatta cctataacca atggctgttg
      181 gttggacgca agacttttga atcaatggga gcattaccca accgaaagta tgcggtcgta
      241 acacgttcaa gttttacatc tgacaatgag aacgtattga tctttccatc aattaaagat
      301 gctttaacca acctaaagaa aataacggat catgtcattg tttcaggtgg tggggagata
      361 tacaaaagcc tgatcgatca agtagataca ctacatatat ctacaataga catcgagccg
      421 gaaggtgatg tttactttcc tgaaatcccc agcaatttta ggccagtttt tacccaagac
      481 ttcgcctcta acataaatta tagttaccaa atctggcaaa agggttaaca agtggcagca
      541 acggattcgc aaacctgtca cgccttttgt accaaaagcc gcgccaggtt tgcgatccgc
      601 tgtgccaggc gttaggcgtc atatgaagat ttcggtgatc cctgagcagg tggcggaaac
      661 attggatgct gagaaccatt tcattgttcg tgaagtgttc gatgtgcacc tatccgacca
      721 aggctttgaa ctatctacca gaagtgtgag cccctaccgg aaggattaca tctcggatga
      781 tgactctgat gaagactctg cttgctatgg cgcattcatc gaccaagagc ttgtcgggaa
      841 gattgaactc aactcaacat ggaacgatct agcctctatc gaacacattg ttgtgtcgca
      901 cacgcaccga ggcaaaggag tcgcgcacag tctcatcgaa tttgcgaaaa agtgggcact
      961 aagcagacag ctccttggca tacgattaga gacacaaacg aacaatgtac ctgcctgcaa
     1021 tttgtacgca aaatgtggct ttactctcgg cggcattgac ctgttcacgt ataaaactag
     1081 acctcaagtc tcgaacgaaa cagcgatgta ctggtactgg ttctcgggag cacaggatga
     1141 cgcctaacaa ttcattcaag ccgacaccgc ttcgcggcgc ggcttaattc aggagttaaa
     1201 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagtt
//
LOCUS       DAJTYM010000463         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.268, whole genome shotgun sequence.
ACCESSION   DAJTYM010000463 DAJTYM010000000
VERSION     DAJTYM010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.268"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..281
                     /locus_tag="OO575_004360"
     CDS             <1..281
                     /locus_tag="OO575_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6667057.1"
                     /translation="LASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQT
                     NNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQDDA"
     gene            339..1130
                     /gene="aadA1"
                     /locus_tag="OO575_004361"
     CDS             339..1130
                     /gene="aadA1"
                     /locus_tag="OO575_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6667058.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          305 a          300 c          318 g          267 t
ORIGIN      
        1 atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc
       61 acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat
      121 tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc
      181 tcggcggcat tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga
      241 tgtactggta ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca
      301 ccgcttcgcg gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgccg
      361 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
      421 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
      481 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
      541 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
      601 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
      661 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
      721 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
      781 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
      841 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
      901 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
      961 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     1021 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     1081 aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa
     1141 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
//
LOCUS       DAJTYM010000464         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.269, whole genome shotgun sequence.
ACCESSION   DAJTYM010000464 DAJTYM010000000
VERSION     DAJTYM010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.269"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..281
                     /locus_tag="OO575_004362"
     CDS             <1..281
                     /locus_tag="OO575_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6667059.1"
                     /translation="LASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQT
                     NNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQDDA"
     gene            339..1130
                     /gene="aadA1"
                     /locus_tag="OO575_004363"
     CDS             339..1130
                     /gene="aadA1"
                     /locus_tag="OO575_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6667060.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREYSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          305 a          299 c          318 g          268 t
ORIGIN      
        1 atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc
       61 acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat
      121 tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc
      181 tcggcggcat tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga
      241 tgtactggta ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca
      301 ccgcttcgcg gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgccg
      361 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
      421 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
      481 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
      541 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
      601 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
      661 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
      721 acattgatct ggctatcttg ctgacaaaag caagagaata tagcgttgcc ttggtaggtc
      781 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
      841 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
      901 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
      961 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     1021 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     1081 aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa
     1141 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
//
LOCUS       DAJTYM010000465         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.270, whole genome shotgun sequence.
ACCESSION   DAJTYM010000465 DAJTYM010000000
VERSION     DAJTYM010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.270"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..281
                     /locus_tag="OO575_004364"
     CDS             <1..281
                     /locus_tag="OO575_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6667061.1"
                     /translation="LASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQT
                     NNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQDDA"
     gene            339..1130
                     /gene="aadA1"
                     /locus_tag="OO575_004365"
     CDS             339..1130
                     /gene="aadA1"
                     /locus_tag="OO575_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6667062.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTEAREYSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          304 a          299 c          319 g          268 t
ORIGIN      
        1 atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc
       61 acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat
      121 tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc
      181 tcggcggcat tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga
      241 tgtactggta ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca
      301 ccgcttcgcg gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgccg
      361 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
      421 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
      481 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
      541 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
      601 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
      661 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
      721 acattgatct ggctatcttg ctgacagaag caagagaata tagcgttgcc ttggtaggtc
      781 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
      841 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
      901 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
      961 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     1021 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     1081 aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa
     1141 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
//
LOCUS       DAJTYM010000466         1187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.271, whole genome shotgun sequence.
ACCESSION   DAJTYM010000466 DAJTYM010000000
VERSION     DAJTYM010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.271"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..281
                     /locus_tag="OO575_004366"
     CDS             <1..281
                     /locus_tag="OO575_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6667063.1"
                     /translation="LASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQT
                     NNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQDDA"
     gene            339..1127
                     /gene="aadA1"
                     /locus_tag="OO575_004367"
     CDS             339..1127
                     /gene="aadA1"
                     /locus_tag="OO575_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6667064.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          302 a          300 c          318 g          267 t
ORIGIN      
        1 atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc
       61 acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat
      121 tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc
      181 tcggcggcat tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga
      241 tgtactggta ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca
      301 ccgcttcgcg gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgccg
      361 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
      421 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
      481 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
      541 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
      601 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
      661 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
      721 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
      781 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
      841 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
      901 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
      961 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     1021 ggcaggctta tcttggacaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     1081 ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc
     1141 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttaga
//
LOCUS       DAJTYM010000467         1187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.272, whole genome shotgun sequence.
ACCESSION   DAJTYM010000467 DAJTYM010000000
VERSION     DAJTYM010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.272"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..281
                     /locus_tag="OO575_004368"
     CDS             <1..281
                     /locus_tag="OO575_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6667065.1"
                     /translation="LASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQT
                     NNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQDDA"
     gene            339..1127
                     /gene="aadA1"
                     /locus_tag="OO575_004369"
     CDS             339..1127
                     /gene="aadA1"
                     /locus_tag="OO575_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6667066.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREYSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          302 a          299 c          318 g          268 t
ORIGIN      
        1 atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc
       61 acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat
      121 tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc
      181 tcggcggcat tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga
      241 tgtactggta ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca
      301 ccgcttcgcg gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgccg
      361 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
      421 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
      481 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
      541 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
      601 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
      661 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
      721 acattgatct ggctatcttg ctgacaaaag caagagaata tagcgttgcc ttggtaggtc
      781 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
      841 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
      901 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
      961 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     1021 ggcaggctta tcttggacaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     1081 ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc
     1141 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttaga
//
LOCUS       DAJTYM010000468         1187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.273, whole genome shotgun sequence.
ACCESSION   DAJTYM010000468 DAJTYM010000000
VERSION     DAJTYM010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.273"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..281
                     /locus_tag="OO575_004370"
     CDS             <1..281
                     /locus_tag="OO575_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6667067.1"
                     /translation="LASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQT
                     NNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQDDA"
     gene            339..1127
                     /locus_tag="OO575_004371"
     CDS             339..1127
                     /locus_tag="OO575_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCR6667068.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTEAREYSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          301 a          299 c          319 g          268 t
ORIGIN      
        1 atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc
       61 acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat
      121 tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc
      181 tcggcggcat tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga
      241 tgtactggta ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca
      301 ccgcttcgcg gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgccg
      361 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
      421 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
      481 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
      541 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
      601 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
      661 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
      721 acattgatct ggctatcttg ctgacagaag caagagaata tagcgttgcc ttggtaggtc
      781 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
      841 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
      901 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
      961 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     1021 ggcaggctta tcttggacaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     1081 ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc
     1141 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttaga
//
LOCUS       DAJTYM010000469         1151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.277, whole genome shotgun sequence.
ACCESSION   DAJTYM010000469 DAJTYM010000000
VERSION     DAJTYM010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.277"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..187
                     /locus_tag="OO575_004372"
     CDS             <1..187
                     /locus_tag="OO575_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667069.1"
                     /translation="TGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTS
                     SIKAKKNAITILNTLNL"
     gene            300..1091
                     /gene="aadA1"
                     /locus_tag="OO575_004373"
     CDS             300..1091
                     /gene="aadA1"
                     /locus_tag="OO575_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6667070.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          312 a          279 c          295 g          265 t
ORIGIN      
        1 aactggtgca ggattcacag caaatagaac cttacaaaac ggatggtttg aagggtttat
       61 tataagcaaa tcaggacata aatatgtttt tgtgtccgca cttacaggaa acttggggtc
      121 gaatttaaca tcaagcataa aagccaagaa aaatgcgatc accattctaa acacactaaa
      181 tttataaaaa atctaatggc aaaatcgccc aacccttcaa tcaagtcggg acggccaaaa
      241 gcaagctttt ggctcccctc gctggcgctc ggcgcccctt atttcaaacg ttaaacatca
      301 tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      361 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      421 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      481 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      541 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      601 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
      661 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
      721 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
      781 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
      841 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
      901 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
      961 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     1021 cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag
     1081 tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     1141 caagcgttag a
//
LOCUS       DAJTYM010000470         1151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.278, whole genome shotgun sequence.
ACCESSION   DAJTYM010000470 DAJTYM010000000
VERSION     DAJTYM010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.278"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..187
                     /locus_tag="OO575_004374"
     CDS             <1..187
                     /locus_tag="OO575_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667071.1"
                     /translation="TGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTS
                     SIKAKKNAITILNTLNL"
     gene            300..1091
                     /gene="aadA1"
                     /locus_tag="OO575_004375"
     CDS             300..1091
                     /gene="aadA1"
                     /locus_tag="OO575_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6667072.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREYSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          312 a          278 c          295 g          266 t
ORIGIN      
        1 aactggtgca ggattcacag caaatagaac cttacaaaac ggatggtttg aagggtttat
       61 tataagcaaa tcaggacata aatatgtttt tgtgtccgca cttacaggaa acttggggtc
      121 gaatttaaca tcaagcataa aagccaagaa aaatgcgatc accattctaa acacactaaa
      181 tttataaaaa atctaatggc aaaatcgccc aacccttcaa tcaagtcggg acggccaaaa
      241 gcaagctttt ggctcccctc gctggcgctc ggcgcccctt atttcaaacg ttaaacatca
      301 tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      361 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      421 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      481 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      541 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      601 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
      661 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaat
      721 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
      781 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
      841 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
      901 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
      961 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     1021 cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag
     1081 tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     1141 caagcgttag a
//
LOCUS       DAJTYM010000471         1151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.279, whole genome shotgun sequence.
ACCESSION   DAJTYM010000471 DAJTYM010000000
VERSION     DAJTYM010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.279"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..187
                     /locus_tag="OO575_004376"
     CDS             <1..187
                     /locus_tag="OO575_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667073.1"
                     /translation="TGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTS
                     SIKAKKNAITILNTLNL"
     gene            300..1091
                     /gene="aadA1"
                     /locus_tag="OO575_004377"
     CDS             300..1091
                     /gene="aadA1"
                     /locus_tag="OO575_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6667074.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTEAREYSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          311 a          278 c          296 g          266 t
ORIGIN      
        1 aactggtgca ggattcacag caaatagaac cttacaaaac ggatggtttg aagggtttat
       61 tataagcaaa tcaggacata aatatgtttt tgtgtccgca cttacaggaa acttggggtc
      121 gaatttaaca tcaagcataa aagccaagaa aaatgcgatc accattctaa acacactaaa
      181 tttataaaaa atctaatggc aaaatcgccc aacccttcaa tcaagtcggg acggccaaaa
      241 gcaagctttt ggctcccctc gctggcgctc ggcgcccctt atttcaaacg ttaaacatca
      301 tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      361 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      421 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      481 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      541 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      601 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
      661 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacagaa gcaagagaat
      721 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
      781 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
      841 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
      901 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
      961 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     1021 cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag
     1081 tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     1141 caagcgttag a
//
LOCUS       DAJTYM010000472         1148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.280, whole genome shotgun sequence.
ACCESSION   DAJTYM010000472 DAJTYM010000000
VERSION     DAJTYM010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.280"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..187
                     /locus_tag="OO575_004378"
     CDS             <1..187
                     /locus_tag="OO575_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667075.1"
                     /translation="TGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTS
                     SIKAKKNAITILNTLNL"
     gene            300..1088
                     /gene="aadA1"
                     /locus_tag="OO575_004379"
     CDS             300..1088
                     /gene="aadA1"
                     /locus_tag="OO575_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6667076.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          309 a          279 c          295 g          265 t
ORIGIN      
        1 aactggtgca ggattcacag caaatagaac cttacaaaac ggatggtttg aagggtttat
       61 tataagcaaa tcaggacata aatatgtttt tgtgtccgca cttacaggaa acttggggtc
      121 gaatttaaca tcaagcataa aagccaagaa aaatgcgatc accattctaa acacactaaa
      181 tttataaaaa atctaatggc aaaatcgccc aacccttcaa tcaagtcggg acggccaaaa
      241 gcaagctttt ggctcccctc gctggcgctc ggcgcccctt atttcaaacg ttaaacatca
      301 tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      361 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      421 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      481 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      541 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      601 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
      661 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
      721 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
      781 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
      841 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
      901 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
      961 agcccgtcat acttgaagct aggcaggctt atcttggaca agaagatcgc ttggcctcgc
     1021 gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg
     1081 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     1141 gcgttaga
//
LOCUS       DAJTYM010000473         1148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.281, whole genome shotgun sequence.
ACCESSION   DAJTYM010000473 DAJTYM010000000
VERSION     DAJTYM010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.281"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..187
                     /locus_tag="OO575_004380"
     CDS             <1..187
                     /locus_tag="OO575_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667077.1"
                     /translation="TGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTS
                     SIKAKKNAITILNTLNL"
     gene            300..1088
                     /gene="aadA1"
                     /locus_tag="OO575_004381"
     CDS             300..1088
                     /gene="aadA1"
                     /locus_tag="OO575_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6667078.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREYSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          309 a          278 c          295 g          266 t
ORIGIN      
        1 aactggtgca ggattcacag caaatagaac cttacaaaac ggatggtttg aagggtttat
       61 tataagcaaa tcaggacata aatatgtttt tgtgtccgca cttacaggaa acttggggtc
      121 gaatttaaca tcaagcataa aagccaagaa aaatgcgatc accattctaa acacactaaa
      181 tttataaaaa atctaatggc aaaatcgccc aacccttcaa tcaagtcggg acggccaaaa
      241 gcaagctttt ggctcccctc gctggcgctc ggcgcccctt atttcaaacg ttaaacatca
      301 tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      361 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      421 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      481 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      541 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      601 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
      661 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaat
      721 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
      781 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
      841 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
      901 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
      961 agcccgtcat acttgaagct aggcaggctt atcttggaca agaagatcgc ttggcctcgc
     1021 gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg
     1081 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     1141 gcgttaga
//
LOCUS       DAJTYM010000474         1148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.282, whole genome shotgun sequence.
ACCESSION   DAJTYM010000474 DAJTYM010000000
VERSION     DAJTYM010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.282"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            <1..187
                     /locus_tag="OO575_004382"
     CDS             <1..187
                     /locus_tag="OO575_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6667079.1"
                     /translation="TGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTS
                     SIKAKKNAITILNTLNL"
     gene            300..1088
                     /locus_tag="OO575_004383"
     CDS             300..1088
                     /locus_tag="OO575_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCR6667080.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTEAREYSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          308 a          278 c          296 g          266 t
ORIGIN      
        1 aactggtgca ggattcacag caaatagaac cttacaaaac ggatggtttg aagggtttat
       61 tataagcaaa tcaggacata aatatgtttt tgtgtccgca cttacaggaa acttggggtc
      121 gaatttaaca tcaagcataa aagccaagaa aaatgcgatc accattctaa acacactaaa
      181 tttataaaaa atctaatggc aaaatcgccc aacccttcaa tcaagtcggg acggccaaaa
      241 gcaagctttt ggctcccctc gctggcgctc ggcgcccctt atttcaaacg ttaaacatca
      301 tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      361 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      421 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      481 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      541 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      601 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
      661 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacagaa gcaagagaat
      721 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
      781 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
      841 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
      901 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
      961 agcccgtcat acttgaagct aggcaggctt atcttggaca agaagatcgc ttggcctcgc
     1021 gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg
     1081 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     1141 gcgttaga
//
LOCUS       DAJTYM010000475         1086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600794
            SAMEA8900434-rid15816053.guided.290, whole genome shotgun sequence.
ACCESSION   DAJTYM010000475 DAJTYM010000000
VERSION     DAJTYM010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900434
            Sequence Read Archive: ERR6004816
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477730.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477730.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:50:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,384
            CDSs (total)                      :: 4,320
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 170 of 357
            Pseudo Genes (incomplete)         :: 84 of 357
            Pseudo Genes (internal stop)      :: 137 of 357
            Pseudo Genes (multiple problems)  :: 33 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900434-rid15816053.guided.290"
                     /serovar="S. flexneri 2a"
                     /isolate="600794"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-15"
     gene            245..904
                     /locus_tag="OO575_004384"
     CDS             245..904
                     /locus_tag="OO575_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6667081.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          294 a          232 c          257 g          303 t
ORIGIN      
        1 cagtaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg tgacggaaga
       61 tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa
      121 cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga
      181 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
      241 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
      301 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
      361 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
      421 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
      481 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
      541 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
      601 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
      661 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
      721 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
      781 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
      841 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
      901 gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggttgctacg cctgaataag
      961 tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca
     1021 gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc
     1081 ggaagc
//
